Cut site frequencies

Cut site frequencies

Enzyme		Specificity

AarI		CACCTGC
AatII		GACGTC
AccI		GTMKAC
AceIII		CAGCTC
AciI		CCGC
AclI		AACGTT
AcyI		GRCGYC
AflII		CTTAAG
AflIII		ACRYGT
AgeI		ACCGGT
AhaIII		TTTAAA
AloI		GAACNNNNNNTCC
AluI		AGCT
AlwNI		CAGNNNCTG
ApaBI		GCANNNNNTGC
ApaI		GGGCCC
ApaLI		GTGCAC
ApoI		RAATTY
AscI		GGCGCGCC
AsuI		GGNCC
AsuII		TTCGAA
AvaI		CYCGRG
AvaII		GGWCC
AvaIII		ATGCAT
AvrII		CCTAGG
BaeI		ACNNNNGTAYC
BalI		TGGCCA
BamHI		GGATCC
BbvCI		CCTCAGC
BbvI		GCAGC
BbvII		GAAGAC
BccI		CCATC
Bce83I		CTTGAG
BcefI		ACGGC
BcgI		CGANNNNNNTGC
BciVI		GTATCC
BclI		TGATCA
BetI		WCCGGW
BfiI		ACTGGG
BglI		GCCNNNNNGGC
BglII		AGATCT
BinI		GGATC
BmgI		GKGCCC
BplI		GAGNNNNNCTC
Bpu10I		CCTNAGC
BsaAI		YACGTR
BsaBI		GATNNNNATC
BsaXI		ACNNNNNCTCC
BsbI		CAACAC
BscGI		CCCGT
BseMII		CTCAG
BsePI		GCGCGC
BseRI		GAGGAG
BseSI		GKGCMC
BsgI		GTGCAG
BsiI		CACGAG
BsiYI		CCNNNNNNNGG
BsmAI		GTCTC
BsmI		GAATGC
Bsp1407I		TGTACA
Bsp24I		GACNNNNNNTGG
BspGI		CTGGAC
BspHI		TCATGA
BspLU11I		ACATGT
BspMI		ACCTGC
BspMII		TCCGGA
BspNCI		CCAGA
BsrBI		CCGCTC
BsrDI		GCAATG
BsrI		ACTGG
BstEII		GGTNACC
BstXI		CCANNNNNNTGG
BtrI		CACGTC
BtsI		GCAGTG
Cac8I		GCNNGC
CauII		CCSGG
Cfr10I		RCCGGY
CfrAI		GCANNNNNNNNGTGG
CfrI		YGGCCR
CjeI		CCANNNNNNGT
CjePI		CCANNNNNNNTC
ClaI		ATCGAT
CviJI		RGCY
CviRI		TGCA
DdeI		CTNAG
DpnI		GATC

Enzyme		Specificity

DraII		RGGNCCY
DraIII		CACNNNGTG
DrdI		GACNNNNNNGTC
DrdII		GAACCA
DsaI		CCRYGG
Eam1105I		GACNNNNNGTC
EciI		GGCGGA
Eco31I		GGTCTC
Eco47III		AGCGCT
Eco57I		CTGAAG
Eco57MI		CTGRAG
EcoAI		GAGNNNNNNNGTCA
EcoBI		TGANNNNNNNNTGCT
EcoDI		TTANNNNNNNGTCY
EcoDR2		TCANNNNNNGTCG
EcoDR3		TCANNNNNNNATCG
EcoDXXI		TCANNNNNNNRTTC
EcoEI		GAGNNNNNNNATGC
EcoKI		AACNNNNNNGTGC
EcoNI		CCTNNNNNAGG
EcoP15I		CAGCAG
EcoPI		AGACC
EcoprrI		CCANNNNNNNRTGC
EcoR124I		GAANNNNNNRTCG
EcoR124II		GAANNNNNNNRTCG
EcoRD2		GAANNNNNNRTTC
EcoRD3		GAANNNNNNNRTTC
EcoRI		GAATTC
EcoRII		CCWGG
EcoRV		GATATC
Esp3I		CGTCTC
EspI		GCTNAGC
FauI		CCCGC
FinI		GGGAC
Fnu4HI		GCNGC
FnuDII		CGCG
FokI		GGATG
FseI		GGCCGGCC
FspAI		RTGCGCAY
GdiII		CGGCCR
GsuI		CTGGAG
HaeI		WGGCCW
HaeII		RGCGCY
HaeIII		GGCC
HaeIV		GAYNNNNNRTC
HgaI		GACGC
HgiAI		GWGCWC
HgiCI		GGYRCC
HgiEII		ACCNNNNNNGGT
HgiJII		GRGCYC
HhaI		GCGC
Hin4I		GAYNNNNNVTC
Hin4II		CCTTC
HindI		CAC
HindII		GTYRAC
HindIII		AAGCTT
HinfI		GANTC
HinfIII		CGAAT
HpaI		GTTAAC
HpaII		CCGG
HphI		GGTGA
Hpy178III		TCNNGA
Hpy188I		TCNGA
Hpy99I		CGWCG
KpnI		GGTACC
Ksp632I		CTCTTC
MaeI		CTAG
MaeII		ACGT
MaeIII		GTNAC
MboI		GATC
MboII		GAAGA
McrI		CGRYCG
MfeI		CAATTG
MjaIV		GTNNAC
MluI		ACGCGT
MmeI		TCCRAC
MnlI		CCTC
MseI		TTAA
MslI		CAYNNNNRTG
MstI		TGCGCA
MwoI		GCNNNNNNNGC
NaeI		GCCGGC
NarI		GGCGCC
NcoI		CCATGG
NdeI		CATATG
NheI		GCTAGC

Enzyme		Specificity

NlaIII		CATG
NlaIV		GGNNCC
NotI		GCGGCCGC
NruI		TCGCGA
NspBII		CMGCKG
NspI		RCATGY
OliI		CACNNNNGTG
PacI		TTAATTAA
Pfl1108I		TCGTAG
PflMI		CCANNNNNTGG
PleI		GAGTC
PmaCI		CACGTG
PmeI		GTTTAAAC
PpiI		GAACNNNNNCTC
PpuMI		RGGWCCY
PshAI		GACNNNNGTC
PsiI		TTATAA
PsrI		GAACNNNNNNTAC
PstI		CTGCAG
PvuI		CGATCG
PvuII		CAGCTG
RleAI		CCCACA
RsaI		GTAC
RsrII		CGGWCCG
SacI		GAGCTC
SacII		CCGCGG
SalI		GTCGAC
SanDI		GGGWCCC
SapI		GCTCTTC
SauI		CCTNAGG
ScaI		AGTACT
ScrFI		CCNGG
SduI		GDGCHC
SecI		CCNNGG
SexAI		ACCWGGT
SfaNI		GCATC
SfeI		CTRYAG
SfiI		GGCCNNNNNGGCC
SgfI		GCGATCGC
SgrAI		CRCCGGYG
SimI		GGGTC
SmaI		CCCGGG
SmlI		CTYRAG
SnaBI		TACGTA
SnaI		GTATAC
SpeI		ACTAGT
SphI		GCATGC
SplI		CGTACG
SrfI		GCCCGGGC
Sse232I		CGCCGGCG
Sse8387I		CCTGCAGG
Sse8647I		AGGWCCT
SspI		AATATT
Sth132I		CCCG
StuI		AGGCCT
StyI		CCWWGG
StyLTI		CAGAG
StyLTIII		GAGNNNNNNRTAYG
StySJI		GAGNNNNNNGTRC
StySKI		CGATNNNNNNNGTTA
StySPI		AACNNNNNNGTRC
StySQI		AACNNNNNNRTAYG
SwaI		ATTTAAAT
TaqI		TCGA
TaqII		CACCCA
TaqII		GACCGA
TatI		WGTACW
TauI		GCSGC
TfiI		GAWTC
TseI		GCWGC
Tsp45I		GTSAC
Tsp4CI		ACNGT
TspEI		AATT
TspGWI		ACGGA
TspRI		CASTGNN
Tth111I		GACNNNGTC
Tth111II		CAARCA
UbaPI		CGAACG
VspI		ATTAAT
XbaI		TCTAGA
XcmI		CCANNNNNNNNNTGG
XhoI		CTCGAG
XhoII		RGATCY
XmaIII		CGGCCG
XmnI		GAANNNNTTC