Expected fragment lengths in PROTOZOA
For prototype restriction enzymes, recognition sites are counted, and the count is divided by genome length.
Expected fragment lengths are listed in base pair units, while genome sizes in million base pair units.
More on restriction enzymes in REBASE.
For more detail contact me at New England Biolabs Posfai@neb.com.
|
Enzyme |
Specificity |
|
|
|
Cryptosporidium parvum |
Dictyostelium discoideum |
Leishmania major |
Plasmodium falciparum |
Theileria parva |
Trypanosoma brucei |
|
|
9.1 M bp |
34.0 M bp |
32.8 M bp |
23.3 M bp |
4.6 M bp |
26.1 M bp |
|
AclI | AACGTT |
9250 |
8090 |
9828 |
6265 |
3957 |
4033 |
HindIII | AAGCTT |
1823 |
7831 |
9093 |
7092 |
3743 |
4667 |
SspI | AATATT |
387 |
377 |
34471 |
273 |
641 |
2117 |
MluCI | AATT |
59 |
45 |
1735 |
65 |
72 |
237 |
PciI | ACATGT |
5105 |
6090 |
4559 |
2525 |
3458 |
3329 |
AgeI | ACCGGT |
15720 |
39096 |
6395 |
96959 |
15587 |
6478 |
BspMI | ACCTGC |
7746 |
7132 |
1290 |
14635 |
4796 |
2196 |
SexAI | ACCWGGT |
21569 |
9654 |
14042 |
43906 |
9194 |
15098 |
MluI | ACGCGT |
75225 |
354308 |
2585 |
136083 |
32744 |
5758 |
BceAI | ACGGC |
6154 |
12869 |
267 |
14672 |
2065 |
566 |
MaeII | ACGT |
1119 |
1392 |
272 |
883 |
503 |
323 |
HpyCH4III | ACNGT |
682 |
960 |
350 |
1146 |
306 |
268 |
AflIII | ACRYGT |
2952 |
4227 |
784 |
1878 |
1830 |
1031 |
SpeI | ACTAGT |
5550 |
9575 |
30842 |
11491 |
3667 |
11119 |
BsrI | ACTGG |
963 |
1322 |
731 |
3931 |
560 |
637 |
BfiI | ACTGGG |
6307 |
22072 |
3534 |
28979 |
2992 |
3029 |
BglII | AGATCT |
2742 |
6426 |
11438 |
9026 |
5431 |
10864 |
AfeI | AGCGCT |
26537 |
125976 |
2326 |
144536 |
17573 |
4601 |
AluI | AGCT |
288 |
971 |
245 |
914 |
391 |
333 |
StuI | AGGCCT |
14379 |
109368 |
6087 |
56895 |
7888 |
10430 |
ScaI | AGTACT |
4139 |
8094 |
8963 |
7463 |
3165 |
6603 |
ClaI | ATCGAT |
6916 |
3943 |
6952 |
8761 |
6330 |
7028 |
TspDTI | ATGAA |
179 |
172 |
729 |
134 |
241 |
322 |
AvaIII | ATGCAT |
2409 |
5279 |
4727 |
2821 |
7002 |
3330 |
AseI | ATTAAT |
477 |
353 |
80040 |
455 |
761 |
4112 |
SwaI | ATTTAAAT |
4194 |
1774 |
1312671 |
2720 |
5007 |
40616 |
MfeI | CAATTG |
2214 |
1187 |
31524 |
7560 |
2934 |
4457 |
BssSI | CACGAG |
15170 |
22001 |
1196 |
36531 |
6980 |
2426 |
BmgBIA | CACGTC |
16732 |
28800 |
1196 |
25404 |
6234 |
2649 |
PmlI | CACGTG |
35417 |
25009 |
2041 |
36190 |
16857 |
3539 |
PvuII | CAGCTG |
9111 |
15252 |
1382 |
26264 |
6012 |
2927 |
BtsIMutIA | CAGTG |
1621 |
1968 |
376 |
3717 |
600 |
397 |
TspRI | CASTG |
1621 |
1968 |
376 |
3717 |
600 |
397 |
NdeI | CATATG |
4620 |
4512 |
10023 |
1762 |
4878 |
3655 |
CviAII FatI NlaIII | CATG |
413 |
696 |
228 |
448 |
417 |
260 |
BspNCI | CCAGA |
619 |
1192 |
711 |
2482 |
543 |
846 |
BccI | CCATC |
757 |
643 |
363 |
1523 |
763 |
469 |
NcoI | CCATGG |
11033 |
14648 |
4333 |
24572 |
8397 |
5241 |
BseYIA | CCCAGC |
10771 |
37667 |
1581 |
56481 |
6634 |
3014 |
FauI | CCCGC |
4653 |
33087 |
312 |
34992 |
4602 |
673 |
SmaI XmaI | CCCGGG |
128201 |
336768 |
8554 |
684420 |
32052 |
12399 |
BscGI | CCCGT |
6268 |
21021 |
581 |
12404 |
1961 |
755 |
AciI | CCGC |
1151 |
3369 |
58 |
4920 |
787 |
131 |
SacII | CCGCGG |
325083 |
1259762 |
1859 |
802424 |
82754 |
5321 |
BsrBI | CCGCTC |
20593 |
61065 |
942 |
111341 |
11238 |
2166 |
HpaII | CCGG |
2646 |
9180 |
277 |
10808 |
1318 |
493 |
ScrFI SsoII | CCNGG |
1382 |
2981 |
361 |
5483 |
708 |
589 |
BtgI | CCRYGG |
8274 |
11909 |
667 |
19311 |
4269 |
1246 |
|
|
|
Cryptosporidium parvum |
Dictyostelium discoideum |
Leishmania major |
Plasmodium falciparum |
Theileria parva |
Trypanosoma brucei |
|
BcnI | CCSGG |
10215 |
36811 |
766 |
39109 |
3288 |
1286 |
AvrII | CCTAGG |
39065 |
163526 |
29942 |
46540 |
14922 |
32553 |
MnlI | CCTC |
244 |
517 |
65 |
599 |
181 |
104 |
BbvCIA | CCTCAGC |
25354 |
101837 |
3693 |
116351 |
12893 |
5802 |
SbfI | CCTGCAGG |
337123 |
971816 |
29779 |
2327030 |
227574 |
65352 |
Bpu10IA | CCTNAGC |
6843 |
41429 |
2181 |
23017 |
4020 |
2824 |
Bsu36I | CCTNAGG |
18963 |
88118 |
10938 |
27280 |
5918 |
7183 |
Hin4II | CCTTC |
665 |
1348 |
276 |
1162 |
574 |
299 |
BstNI EcoRII | CCWGG |
1598 |
3243 |
684 |
6377 |
903 |
1089 |
StyI | CCWWGG |
2940 |
5968 |
1384 |
7406 |
1947 |
1816 |
PvuI | CGATCG |
57976 |
64419 |
4197 |
108233 |
42938 |
10253 |
FnuDII | CGCG |
5434 |
27320 |
114 |
18294 |
3603 |
364 |
EagI | CGGCCG |
379263 |
409802 |
1992 |
775676 |
61506 |
6881 |
RsrII | CGGWCCG |
535430 |
872143 |
12501 |
2908788 |
119776 |
22556 |
BsiEI | CGRYCG |
31495 |
36184 |
748 |
61399 |
13876 |
1993 |
BsiWI | CGTACG |
130033 |
216647 |
3596 |
73873 |
26931 |
6112 |
Esp3I | CGTCTC |
10192 |
37709 |
1013 |
31110 |
7294 |
2543 |
Hpy99I | CGWCG |
9481 |
11111 |
221 |
10758 |
2099 |
675 |
MspA1I | CMGCKG |
6752 |
9893 |
346 |
18751 |
3485 |
806 |
SgrAI | CRCCGGYG |
159689 |
548606 |
3242 |
802424 |
79850 |
16185 |
BfaIA | CTAG |
540 |
1695 |
1061 |
1331 |
516 |
875 |
XhoI | CTCGAG |
17437 |
74591 |
2843 |
109765 |
10025 |
7022 |
CatHI | CTCTTC |
1224 |
2389 |
716 |
2641 |
1550 |
960 |
PstI | CTGCAG |
8677 |
28703 |
1252 |
38654 |
7860 |
2203 |
DdeI | CTNAG |
429 |
2020 |
475 |
1141 |
315 |
426 |
SfeI | CTRYAG |
1873 |
4331 |
893 |
3713 |
1395 |
1135 |
AflII | CTTAAG |
4403 |
25383 |
31985 |
6710 |
5108 |
8328 |
SmlI | CTYRAG |
1236 |
3562 |
1248 |
3833 |
1176 |
1442 |
AvaI | CYCGRG |
10024 |
40015 |
1205 |
55142 |
4013 |
2229 |
MboII | GAAGA |
217 |
370 |
273 |
299 |
310 |
272 |
BbsIA | GAAGAC |
2832 |
9060 |
1294 |
5052 |
2562 |
1967 |
BsmI | GAATGC |
2600 |
6191 |
2379 |
7508 |
4368 |
1901 |
EcoRI | GAATTC |
1897 |
3573 |
13307 |
5840 |
3094 |
5288 |
HgaI | GACGC |
5735 |
19260 |
253 |
15003 |
2629 |
676 |
AatII ZraI | GACGTC |
46204 |
111886 |
2959 |
72044 |
13709 |
7247 |
SacI | GAGCTC |
7942 |
55216 |
2825 |
52767 |
10609 |
5943 |
MlyI PleI | GAGTC |
1124 |
3586 |
668 |
6594 |
778 |
873 |
HinfI | GANTC |
281 |
453 |
425 |
900 |
267 |
404 |
BstSEI | GASTC |
1124 |
3586 |
668 |
6594 |
778 |
873 |
EcoRV | GATATC |
4181 |
4274 |
9953 |
6255 |
4003 |
5345 |
BspKT6I MboI | GATC |
361 |
494 |
426 |
808 |
433 |
489 |
TfiI | GAWTC |
375 |
519 |
1171 |
1043 |
407 |
755 |
BsrDIB | GCAATG |
2700 |
4191 |
2007 |
10914 |
4115 |
1586 |
BbvI R1.BceSIV | GCAGC |
1536 |
2578 |
121 |
5064 |
1847 |
302 |
PenI | GCAGT |
1208 |
2156 |
373 |
4445 |
920 |
404 |
BtsIA | GCAGTG |
8178 |
12606 |
872 |
31617 |
4440 |
1252 |
BscAI SfaNI | GCATC |
1036 |
1544 |
307 |
2356 |
1329 |
459 |
SphI | GCATGC |
4672 |
62755 |
1895 |
26323 |
28270 |
3935 |
|
|
|
Cryptosporidium parvum |
Dictyostelium discoideum |
Leishmania major |
Plasmodium falciparum |
Theileria parva |
Trypanosoma brucei |
|
NaeI | GCCGGC |
76490 |
523286 |
1503 |
861863 |
51721 |
6561 |
AsiSI | GCGATCGC |
1300332 |
3092144 |
33114 |
11635152 |
1137873 |
120719 |
HhaI HinP1I | GCGC |
1856 |
10288 |
91 |
10477 |
2225 |
291 |
McaTI NmeM395I | GCGCGC |
29457 |
680271 |
892 |
528870 |
182059 |
5426 |
NotI | GCGGCCGC |
3034108 |
11337863 |
9362 |
3878384 |
4551493 |
48467 |
BthCI Fnu4HI | GCNGC |
1261 |
2189 |
65 |
4307 |
1309 |
179 |
AspCNI | GCSGC |
7050 |
14523 |
142 |
28835 |
4493 |
443 |
NheI | GCTAGC |
22927 |
146610 |
7387 |
26117 |
42537 |
10290 |
SapI | GCTCTTC |
7585 |
42200 |
2884 |
28907 |
12469 |
5248 |
BlpI | GCTNAGC |
15045 |
122792 |
2906 |
96557 |
16733 |
6022 |
TseI | GCWGC |
1536 |
2578 |
121 |
5064 |
1847 |
302 |
Bsp1286I | GDGCHC |
2491 |
5869 |
293 |
5681 |
1780 |
583 |
AlwI | GGATC |
884 |
1396 |
1007 |
2429 |
882 |
909 |
BamHI | GGATCC |
10733 |
23265 |
10997 |
28764 |
7675 |
7083 |
FokI StsI | GGATG |
751 |
1285 |
412 |
1307 |
754 |
465 |
HaeIII | GGCC |
1523 |
4514 |
229 |
7101 |
912 |
479 |
| |
0 |
0 |
0 |
0 |
0 |
0 |
KasI NarI | GGCGCC |
7720 |
185866 |
1483 |
261464 |
30753 |
5368 |
AscI | GGCGCGCC |
700178 |
11337863 |
16760 |
23270305 |
1137873 |
99524 |
EciI | GGCGGA |
12929 |
62068 |
955 |
58615 |
9012 |
2169 |
ApaI | GGGCCC |
53859 |
263671 |
5695 |
177635 |
42537 |
9319 |
Eco47II | GGNCC |
1749 |
3013 |
349 |
3231 |
902 |
526 |
KpnI | GGTACC |
10308 |
13860 |
7892 |
23600 |
8702 |
6551 |
Eco31I | GGTCTC |
8828 |
15747 |
2726 |
25942 |
6044 |
3958 |
HphI | GGTGA |
1136 |
518 |
368 |
1665 |
642 |
410 |
BstEII | GGTNACC |
28896 |
17587 |
6446 |
36190 |
12040 |
6643 |
AvaII | GGWCC |
2786 |
3615 |
925 |
3850 |
1355 |
1022 |
HgiCI | GGYRCC |
3602 |
6827 |
544 |
13818 |
3847 |
1155 |
BaeGI | GKGCMC |
10426 |
14345 |
623 |
12260 |
5591 |
1272 |
BsaHI | GRCGYC |
5405 |
41837 |
477 |
34628 |
4630 |
1473 |
BanII | GRGCYC |
4585 |
27743 |
1030 |
25487 |
4183 |
1859 |
CviQI PabI RsaI | GTAC |
598 |
699 |
333 |
571 |
368 |
332 |
BstZ17I | GTATAC |
7793 |
9864 |
11312 |
4415 |
4137 |
5899 |
BciVI | GTATCC |
4399 |
7804 |
4542 |
6042 |
3054 |
3430 |
SalI | GTCGAC |
55842 |
81960 |
2587 |
75308 |
12401 |
7118 |
BsmAI | GTCTC |
1200 |
3406 |
441 |
4146 |
1157 |
734 |
ApaLI | GTGCAC |
34091 |
23249 |
1478 |
19424 |
18061 |
3413 |
AccI | GTMKAC |
3589 |
5565 |
1325 |
3045 |
1858 |
1905 |
Tsp45I | GTSAC |
2406 |
2918 |
492 |
3323 |
960 |
538 |
HpaI | GTTAAC |
6958 |
9971 |
30988 |
8099 |
2682 |
5531 |
PmeI | GTTTAAAC |
50012 |
49223 |
800409 |
92710 |
27254 |
73245 |
HindII | GTYRAC |
2836 |
3238 |
1309 |
4218 |
1084 |
1307 |
BsiHKAI | GWGCWC |
3829 |
7719 |
490 |
7617 |
3154 |
1041 |
ApoI | RAATTY |
210 |
176 |
4799 |
271 |
263 |
823 |
NspI | RCATGY |
1380 |
3265 |
686 |
1576 |
1978 |
898 |
Cfr10I NmeDI | RCCGGY |
7867 |
20090 |
655 |
47201 |
6330 |
1598 |
XhoII | RGATCY |
1113 |
2553 |
2544 |
3551 |
1569 |
2073 |
HaeII | RGCGCY |
4539 |
35541 |
428 |
49093 |
5598 |
1179 |
|
|
|
Cryptosporidium parvum |
Dictyostelium discoideum |
Leishmania major |
Plasmodium falciparum |
Theileria parva |
Trypanosoma brucei |
|
CviJI | RGCY |
145 |
472 |
61 |
518 |
143 |
99 |
EcoO109I | RGGNCCY |
8506 |
29732 |
1683 |
7034 |
3927 |
2241 |
PpuMI | RGGWCCY |
13326 |
38004 |
4345 |
7845 |
5503 |
4406 |
SnaBI | TACGTA |
10874 |
22782 |
13022 |
6446 |
6823 |
6758 |
BspHI | TCATGA |
3886 |
5417 |
5391 |
3909 |
4042 |
4570 |
AccIII | TCCGGA |
16950 |
132865 |
11434 |
61237 |
11852 |
7877 |
EsaBC3I TaqI | TCGA |
626 |
846 |
247 |
1043 |
483 |
401 |
NruI | TCGCGA |
55842 |
197753 |
3620 |
165037 |
42143 |
7209 |
Hpy188I | TCNGA |
290 |
664 |
464 |
844 |
272 |
402 |
XbaI | TCTAGA |
3103 |
11251 |
24041 |
11075 |
4471 |
14994 |
BclI | TGATCA |
2520 |
2528 |
6816 |
3166 |
3626 |
5746 |
HpyCH4V | TGCA |
271 |
465 |
162 |
545 |
459 |
180 |
FspI | TGCGCA |
17640 |
88346 |
1388 |
55804 |
30546 |
3273 |
BalI | TGGCCA |
7247 |
12139 |
3722 |
45807 |
7145 |
5578 |
BsrGI | TGTACA |
4938 |
5117 |
4251 |
2152 |
2640 |
3326 |
MseI | TTAA |
76 |
57 |
2255 |
70 |
82 |
277 |
PacI | TTAATTAA |
4338 |
2286 |
2983343 |
4655 |
6347 |
51943 |
PsiI | TTATAA |
1008 |
559 |
58289 |
335 |
924 |
3779 |
BstBI | TTCGAA |
3772 |
6354 |
7922 |
6102 |
4952 |
5266 |
DraI | TTTAAA |
565 |
241 |
36625 |
376 |
568 |
2030 |
PspXI | VCTCGAGB |
42336 |
172657 |
4318 |
280365 |
18653 |
12060 |
BsaWI | WCCGGW |
5282 |
18208 |
1978 |
20095 |
3437 |
1696 |
BsaAI | YACGTR |
3997 |
6104 |
931 |
3419 |
2712 |
1181 |
EaeI | YGGCCR |
5615 |
9260 |
659 |
31193 |
3896 |
1516 |
|
Posfai@neb.com
September 11, 2013