# ENZYME SPECIFICITY NUMBER_OF_SITES AVE_DISTANCE SITES_PER_MEGABASE AatII GACGTC 712 6519 153.4 AccI GTMKAC 1626 2855 350.3 AciI CCGC 57096 81 12300.8 AclI AACGTT 1705 2722 367.3 AflII CTTAAG 557 8333 120.0 AflIII ACRYGT 2595 1789 559.1 AgeI ACCGGT 1816 2556 391.2 AluI AGCT 13340 348 2874.0 ApaI GGGCCC 67 69278 14.4 ApaLI GTGCAC 575 8072 123.9 ApoI RAATTY 5718 812 1231.9 AscI GGCGCGCC 166 27962 35.8 AvaI CYCGRG 1248 3719 268.9 AvaII GGWCC 2875 1614 619.4 AvaIII ATGCAT 840 5526 181.0 AvrII CCTAGG 16 290103 3.4 BamHI GGATCC 494 9396 106.4 BbvI GCAGC 17989 258 3875.6 BccI CCATC 12952 358 2790.4 BceAI ACGGC 12498 371 2692.6 BciVI GTATCC 1455 3190 313.5 BclI TGATCA 1607 2888 346.2 BfiI ACTGGG 2014 2305 433.9 BglII AGATCT 701 6621 151.0 BsaAI YACGTR 1930 2405 415.8 BscAI GCATC 14307 324 3082.3 BscGI CCCGT 6397 726 1378.2 BsmAI GTCTC 2986 1554 643.3 BsmI GAATGC 2656 1748 572.2 BspHI TCATGA 1147 4047 247.1 BspKT6I GATC 19124 243 4120.1 BspMI ACCTGC 3859 1203 831.4 BspNCI CCAGA 15029 309 3237.9 BsrBI CCGCTC 1957 2372 421.6 BsrI ACTGG 14618 318 3149.3 BstEII GGTNACC 1377 3371 296.7 BstNI CCWGG 12050 385 2596.1 BthCI GCNGC 37400 124 8057.5 Cac8I GCNNGC 29937 155 6449.6 ClaI ATCGAT 1421 3266 306.1 CviAII CATG 15264 304 3288.5 CviQI GTAC 12039 386 2593.7 DdeI CTNAG 6791 684 1463.1 EciI GGCGGA 3298 1407 710.5 EcoRI GAATTC 646 7185 139.2 EcoRV GATATC 2041 2274 439.7 EsaBC3I TCGA 15462 300 3331.1 FatI CATG 15264 304 3288.5 FauI CCCGC 11168 416 2406.0 Fnu4HI GCNGC 37400 124 8057.5 FnuDII CGCG 28226 164 6081.0 FokI GGATG 10464 444 2254.4 FseI GGCCGGCC 4 1160413 0.9 HaeII RGCGCY 6520 712 1404.7 HaeIII GGCC 12585 369 2711.3 HgaI GACGC 11769 394 2535.5 HgiCI GGYRCC 3710 1251 799.3 HhaI GCGC 35090 132 7559.8 Hin4II CCTTC 8760 530 1887.3 HindIII AAGCTT 556 8348 119.8 HinfI GANTC 10750 432 2316.0 HinP1I GCGC 35090 132 7559.8 HpaI GTTAAC 1597 2906 344.1 HpaII CCGG 24311 191 5237.6 HphI GGTGA 14735 315 3174.5 Hpy188I TCNGA 12175 381 2623.0 Hpy99I CGWCG 10668 435 2298.3 KasI GGCGCC 93 49910 20.0 KpnI GGTACC 517 8978 111.4 MboI GATC 19124 243 4120.1 MboII GAAGA 12868 361 2772.3 MfeI CAATTG 987 4703 212.6 MjaIV GTNNAC 13011 357 2803.1 MluI ACGCGT 1328 3495 286.1 MlyI GAGTC 3569 1301 768.9 MnlI CCTC 16177 287 3485.2 MseI TTAA 21209 219 4569.3 NaeI GCCGGC 291 15951 62.7 NarI GGCGCC 93 49910 20.0 NcoI CCATGG 613 7572 132.1 NdeI CATATG 683 6796 147.1 NheI GCTAGC 157 29565 33.8 NlaIII CATG 15264 304 3288.5 NlaIV GGNNCC 8471 548 1825.0 NotI GCGGCCGC 23 201811 5.0 NruI TCGCGA 1411 3290 304.0 NspI RCATGY 2787 1665 600.4 PabI GTAC 12039 386 2593.7 PacI TTAATTAA 143 32459 30.8 PleI GAGTC 3569 1301 768.9 PmeI GTTTAAAC 87 53352 18.7 PpuMI RGGWCCY 258 17991 55.6 PsiI TTATAA 868 5348 187.0 PspXI VCTCGAGB 110 42197 23.7 PstI CTGCAG 958 4845 206.4 PvuI CGATCG 1349 3441 290.6 PvuII CAGCTG 1776 2614 382.6 R1.BceSIV GCAGC 17989 258 3875.6 RsaI GTAC 12039 386 2593.7 RsrII CGGWCCG 383 12119 82.5 SacI GAGCTC 152 30537 32.7 SacII CCGCGG 658 7054 141.8 SalI GTCGAC 544 8532 117.2 SapI GCTCTTC 683 6796 147.1 ScaI AGTACT 400 11604 86.2 ScrFI CCNGG 21455 216 4622.3 SexAI ACCWGGT 809 5738 174.3 SfaNI GCATC 14307 324 3082.3 SfeI CTRYAG 2301 2017 495.7 SgrAI CRCCGGYG 548 8470 118.1 SmaI CCCGGG 426 10896 91.8 SmlI CTYRAG 1760 2637 379.2 SnaBI TACGTA 546 8501 117.6 SpeI ACTAGT 78 59508 16.8 SphI GCATGC 588 7894 126.7 SsoII CCNGG 21455 216 4622.3 SspI AATATT 2052 2262 442.1 StuI AGGCCT 606 7659 130.6 StyI CCWWGG 1058 4387 227.9 SwaI ATTTAAAT 117 39672 25.2 TaqI TCGA 15462 300 3331.1 TfiI GAWTC 7181 646 1547.1 TseI GCWGC 17989 258 3875.6 Tsp45I GTSAC 7774 597 1674.8 TspRI CASTG 10352 448 2230.2 XbaI TCTAGA 39 119017 8.4 XhoI CTCGAG 178 26077 38.3 XmaI CCCGGG 426 10896 91.8 ZraI GACGTC 712 6519 153.4