Distribution of restriction sites in the human genome

Enzyme:  FspI               Longest uncut segments
Specificity:  TGCGCA               Repeats in uncut segments
Number of sites:  87588               Genes in uncut segments
Mean distance between sites:  32668 base pairs
Standard deviation:  40144 base pairs
Site density 30.6 per megabase               Help


Distribution of closely spaced sites

Distribution of sites within 7 STD distance


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Longest uncut segments
# Length  Chr  Scaffold  Coordinates  Repeat content Gene content
1   745754  chr4  NT_016354.19  29518364-30264118    56.36 % in   1105 repeats    2.57 % in 1 genes
2   649184  chr3  NT_005612.16  41598983-42248167    58.93 % in   1100 repeats    10.55 % in 1 genes
3   549436  chr2  NT_005403.17  33286709-33836145    49.15 % in   916 repeats    64.78 % in 2 genes
4   539256  chr15  NT_037852.6  1388646-1927902    4.12 % in   87 repeats    1.85 % in 1 genes
5   488569  chr1  NT_032977.9  44439688-44928257    50.37 % in   774 repeats    96.97 % in 3 genes
6   463660  chr6  NT_167244.1  2324312-2787972    6.89 % in   129 repeats    2.82 % in 2 genes
7   460997  chr8  NT_167187.1  31165902-31626899    91.94 % in   237 repeats    0.00 % in 0 genes
8   455302  chr6  NT_025741.15  6172139-6627441    37.94 % in   729 repeats    100.00 % in 1 genes
9   442565  chrX  NT_011786.16  4271809-4714374    51.87 % in   608 repeats    0.00 % in 0 genes
10   439528  chr7  NT_007933.15  20914768-21354296    40.09 % in   675 repeats    0.00 % in 0 genes
11   430362  chrY  NT_011875.12  4350122-4780484    64.67 % in   791 repeats    0.00 % in 0 genes
12   429474  chr21  NT_011512.11  15390326-15819800    51.47 % in   724 repeats    0.00 % in 0 genes
13   428940  chr8  NT_008046.16  36101428-36530368    50.45 % in   738 repeats    0.00 % in 0 genes
14   412278  chr13  NT_009952.14  1006311-1418589    50.31 % in   703 repeats    0.00 % in 0 genes
15   410452  chr2  NT_005403.17  23830494-24240946    38.62 % in   662 repeats    0.00 % in 0 genes
16   407599  chr14  NT_026437.12  40930959-41338558    51.33 % in   643 repeats    0.00 % in 0 genes


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Repeats in longest uncut segments
# Length  Chr  Scaffold  Coordinates  Repeats
Total  Distinct    Most  Second  Third 
745754  chr4  NT_016354.19  29518364-30264118    1105  265       AT_rich (114)  L2a (52)  MIRb (46) 
649184  chr3  NT_005612.16  41598983-42248167    1100  269       L2c (54)  MIRb (47)  L2a (45) 
549436  chr2  NT_005403.17  33286709-33836145    916  236       AT_rich (87)  L2a (44)  MIRb (41) 
539256  chr15  NT_037852.6  1388646-1927902    87  46       AT_rich (10)  L1MDa (6)  L2a (5) 
488569  chr1  NT_032977.9  44439688-44928257    774  210       AT_rich (85)  MIRb (45)  MIR (38) 
463660  chr6  NT_167244.1  2324312-2787972    129  59       AluSx (8)  AluJo (8)  L1MC4a (7) 
460997  chr8  NT_167187.1  31165902-31626899    237  75       ALR/Alpha (51)  MSTA (21)  MSTA-int (15) 
455302  chr6  NT_025741.15  6172139-6627441    729  179       AT_rich (104)  L2a (37)  MIRb (34) 
442565  chrX  NT_011786.16  4271809-4714374    608  187       AluSx (31)  AluY (29)  MIR (22) 
10  439528  chr7  NT_007933.15  20914768-21354296    675  198       AT_rich (89)  L2a (32)  MIR (26) 
11  430362  chrY  NT_011875.12  4350122-4780484    791  190       AT_rich (62)  (TA)n (32)  AluJb (31) 
12  429474  chr21  NT_011512.11  15390326-15819800    724  218       AT_rich (43)  AluSx (25)  MIR (24) 
13  428940  chr8  NT_008046.16  36101428-36530368    738  192       AT_rich (50)  AluSx (40)  MIRb (39) 
14  412278  chr13  NT_009952.14  1006311-1418589    703  213       AT_rich (83)  (TA)n (28)  L2a (26) 
15  410452  chr2  NT_005403.17  23830494-24240946    662  193       MIRb (36)  AT_rich (33)  MIR (29) 
16  407599  chr14  NT_026437.12  40930959-41338558    643  189       AT_rich (42)  MIRb (35)  MIR (32) 


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Genes in longest uncut segments
Sgmnt   Length (bp)  Chr  Scaffold  Coordinates  Gene symbol  Gene function 
1   745754       chr4  NT_016354.19  29518364-30264118    CXXC4  CXXC-type_zinc_finger_protein_4
2   649184       chr3  NT_005612.16  41598983-42248167    LOC100420050  serine/threonine-protein_phosphatase_2A_regulatory_subunit_B''_subunit_alpha_isoform_3
3   549436       chr2  NT_005403.17  33286709-33836145    PDE1A  calcium/calmodulin-dependent_3',5'-cyclic_nucleotide_phosphodiesterase_1A_isoform_2
DNAJC10  dnaJ_homolog_subfamily_C_member_10_precursor
4   539256       chr15  NT_037852.6  1388646-1927902    LOC100418897 
5   488569       chr1  NT_032977.9  44439688-44928257    LRRIQ3  leucine-rich_repeat_and_IQ_domain-containing_protein_3
FPGT  fucose-1-phosphate_guanylyltransferase
TNNI3K  serine/threonine-protein_kinase_TNNI3K_isoform_b
6   463660       chr6  NT_167244.1  2324312-2787972    HCG22  HLA_complex_group_22
MICB  MHC_class_I_polypeptide-related_sequence_B_precursor
8   455302       chr6  NT_025741.15  6172139-6627441    LOC100418924  glutamate_receptor,_ionotropic_kainate_2_isoform_2_precursor



Posfai@neb.com
May 11, 2011