# SEQUENCE TOTAL # Megabase 143.73 # # ENZYME SPEC SITE COUNT AVERAGE SITES PRE # DISTANCE MEGABASE # AatII GACGTC 28240 5089 196 Acc65I GGTACC 19631 7321 136 AccI GTMKAC 129495 1109 900 AciI CCGC 1405437 102 9778 AclI AACGTT 52471 2739 365 AcuI CTGAAG 123354 1165 858 AfeI AGCGCT 42092 3414 292 AflII CTTAAG 61959 2319 431 AflIII ACRYGT 154720 928 1076 AgeI ACCGGT 18868 7617 131 AluI AGCT 1047665 137 7289 AlwI GGATC 354521 405 2466 ApaI GGGCCC 20674 6952 143 ApaLI GTGCAC 56098 2562 390 ApeKI GCWGC 800760 179 5571 ApoI RAATTY 704034 204 4898 AscI GGCGCGCC 733 196079 5 AseI ATTAAT 189580 758 1319 AsiSI GCGATCGC 1625 88446 11 AvaI CYCGRG 111933 1284 778 AvaII GGWCC 283575 506 1973 AvrII CCTAGG 13487 10656 93 BaeGI GKGCMC 161334 890 1122 BamHI GGATCC 42683 3367 296 BanI GGYRCC 143540 1001 998 BanII GRGCYC 135846 1058 945 BbvI GCAGC 800760 179 5571 BccI CCATC 498859 288 3470 BceAI ACGGC 301390 476 2096 BciVI GTATCC 74735 1923 519 BclI TGATCA 59144 2430 411 BcoDI GTCTC 266663 538 1855 BfuAI ACCTGC 88493 1624 615 BfuCI GATC 741325 193 5157 BglII AGATCT 49740 2889 346 BlpI GCTNAGC 58161 2471 404 BmrI ACTGGG 98112 1464 682 BmtI GCTAGC 31415 4575 218 BpmI CTGGAG 133360 1077 927 BpuEI CTTGAG 101606 1414 706 BsaAI YACGTR 130719 1099 909 BsaHI GRCGYC 137850 1042 959 BsaI GGTCTC 59168 2429 411 BsaWI WCCGGW 121885 1179 848 BseRI GAGGAG 161065 892 1120 BsgI GTGCAG 138520 1037 963 BsiEI CGRYCG 119679 1200 832 BsiHKAI GWGCWC 219068 656 1524 BsiWI CGTACG 17316 8300 120 BsmAI GTCTC 266663 538 1855 BsmBI CGTCTC 63636 2258 442 BsmFI GGGAC 276784 519 1925 BsmI GAATGC 154966 927 1078 BsoBI CYCGRG 111933 1284 778 Bsp1286I GDGCHC 387027 371 2692 BspCNI CTCAG 330771 434 2301 BspDI ATCGAT 86639 1658 602 BspEI TCCGGA 41205 3488 286 BspHI TCATGA 41046 3501 285 BspMI ACCTGC 88493 1624 615 BspQI GCTCTTC 37936 3788 263 BsrBI CCGCTC 95647 1502 665 BsrFI RCCGGY 131068 1096 911 BsrGI TGTACA 79979 1797 556 BsrI ACTGG 486562 295 3385 BssHII GCGCGC 25336 5672 176 BssSI CACGAG 60713 2367 422 BstBI TTCGAA 93733 1533 652 BstEII GGTNACC 33099 4342 230 BstNI CCWGG 380908 377 2650 BstUI CGCG 415157 346 2888 BstYI RGATCY 185047 776 1287 BstZ17I GTATAC 44526 3227 309 Bsu36I CCTNAGG 20074 7159 139 BtgI CCRYGG 144419 995 1004 BtgZI GCGATG 113135 1270 787 BtsCI GGATG 451969 317 3144 ClaI ATCGAT 86639 1658 602 CviAII CATG 808424 177 5624 CviKI-1 RGCY 3412254 42 23741 CviQI GTAC 620719 231 4318 DdeI CTNAG 626140 229 4356 DpnII GATC 741325 193 5157 DraI TTTAAA 296420 484 2062 EaeI YGGCCR 325328 441 2263 EagI CGGCCG 28470 5048 198 EarI CTCTTC 136204 1055 947 EciI GGCGGA 116727 1231 812 Eco53kI GAGCTC 52449 2740 364 EcoO109I RGGNCCY 92023 1561 640 EcoRI GAATTC 76998 1866 535 EcoRV GATATC 52034 2762 362 FatI CATG 808424 177 5624 FauI CCCGC 267556 537 1861 Fnu4HI GCNGC 1217865 118 8473 FokI GGATG 451969 317 3144 FseI GGCCGGCC 856 167904 5 FspI TGCGCA 59008 2435 410 HaeII RGCGCY 168813 851 1174 HaeIII GGCC 768948 186 5350 HgaI GACGC 252145 570 1754 HhaI GCGC 621169 231 4321 HincII GTYRAC 183275 784 1275 HindIII AAGCTT 76812 1871 534 HinfI GANTC 759271 189 5282 HinP1I GCGC 621169 231 4321 HpaI GTTAAC 47141 3048 327 HpaII CCGG 499201 287 3473 HphI GGTGA 358689 400 2495 Hpy188I TCNGA 779292 184 5422 Hpy99I CGWCG 279926 513 1947 HpyAV CCTTC 420933 341 2928 HpyCH4III ACNGT 635805 226 4423 HpyCH4IV ACGT 537433 267 3739 HpyCH4V TGCA 1423568 100 9904 KasI GGCGCC 42026 3419 292 KpnI GGTACC 19631 7321 136 MboI GATC 741325 193 5157 MboII GAAGA 576412 249 4010 MfeI CAATTG 116258 1236 808 MluCI AATT 2533383 56 17626 MluI ACGCGT 22579 6365 157 MlyI GAGTC 296747 484 2064 MmeI TCCRAC 189538 758 1318 MnlI CCTC 1393483 103 9695 MscI TGGCCA 163362 879 1136 MseI TTAA 2026173 70 14097 MspA1I CMGCKG 267871 536 1863 MspI CCGG 499201 287 3473 NaeI GCCGGC 53941 2664 375 NarI GGCGCC 42026 3419 292 NciI CCSGG 191934 748 1335 NcoI CCATGG 50326 2855 350 NdeI CATATG 78527 1830 546 NgoMIV GCCGGC 53941 2664 375 NheI GCTAGC 31415 4575 218 NlaIII CATG 808424 177 5624 NmeAIII GCCGAG 99683 1441 693 NotI GCGGCCGC 1444 99533 10 NruI TCGCGA 33388 4304 232 NsiI ATGCAT 93381 1539 649 NspI RCATGY 231000 622 1607 PacI TTAATTAA 16222 8859 112 PaeR7I CTCGAG 39120 3673 272 PciI ACATGT 62668 2293 436 PleI GAGTC 296747 484 2064 PluTI GGCGCC 42026 3419 292 PmeI GTTTAAAC 2640 54441 18 PmlI CACGTG 31876 4508 221 PpuMI RGGWCCY 54611 2631 379 PsiI TTATAA 156765 916 1090 PspGI CCWGG 380908 377 2650 PspOMI GGGCCC 20674 6952 143 PspXI VCTCGAGB 11428 12576 79 PstI CTGCAG 98457 1459 685 PvuI CGATCG 41374 3473 287 PvuII CAGCTG 117954 1218 820 RsaI GTAC 620719 231 4318 RsrII CGGWCCG 9801 14664 68 SacI GAGCTC 52449 2740 364 SacII CCGCGG 21311 6744 148 SalI GTCGAC 33446 4297 232 SapI GCTCTTC 37936 3788 263 Sau3AI GATC 741325 193 5157 Sau96I GGNCC 540139 266 3758 SbfI CCTGCAGG 1975 72772 13 ScaI AGTACT 42274 3399 294 ScrFI CCNGG 572842 250 3985 SexAI ACCWGGT 18648 7707 129 SfaNI GCATC 449733 319 3129 SfcI CTRYAG 197494 727 1374 SfoI GGCGCC 42026 3419 292 SgrAI CRCCGGYG 5718 25135 39 SmaI CCCGGG 19140 7509 133 SmlI CTYRAG 202685 709 1410 SnaBI TACGTA 36540 3933 254 SpeI ACTAGT 28591 5026 198 SphI GCATGC 54656 2629 380 SrfI GCCCGGGC 814 176567 5 SspI AATATT 258956 555 1801 StuI AGGCCT 28242 5089 196 StyD4I CCNGG 572842 250 3985 StyI CCWWGG 176877 812 1230 SwaI ATTTAAAT 19185 7491 133 TaqI TCGA 954768 150 6642 TfiI GAWTC 462524 310 3218 TseI GCWGC 800760 179 5571 Tsp45I GTSAC 294206 488 2046 TspMI CCCGGG 19140 7509 133 TspRI CASTG 487907 294 3394 XbaI TCTAGA 38108 3771 265 XhoI CTCGAG 39120 3673 272 XmaI CCCGGG 19140 7509 133 ZraI GACGTC 28240 5089 196