Fragment size distributions after double digests of the human genome

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# MnlI.MseI (CCTC+TTAA) double digest, 46953 thousand fragments (27739501+19214674 sites), 63 bp average fragment length
   5% of sequence in fragments shorter than      21 bp
  10% of sequence in fragments shorter than      32 bp
  15% of sequence in fragments shorter than      43 bp
  20% of sequence in fragments shorter than      52 bp
  25% of sequence in fragments shorter than      61 bp
  30% of sequence in fragments shorter than      70 bp
  35% of sequence in fragments shorter than      80 bp
  40% of sequence in fragments shorter than      89 bp
  45% of sequence in fragments shorter than      99 bp
  50% of sequence in fragments shorter than     110 bp
  55% of sequence in fragments shorter than     118 bp
  60% of sequence in fragments shorter than     130 bp
  65% of sequence in fragments shorter than     142 bp
  70% of sequence in fragments shorter than     156 bp
  75% of sequence in fragments shorter than     173 bp
  80% of sequence in fragments shorter than     193 bp
  85% of sequence in fragments shorter than     221 bp
  90% of sequence in fragments shorter than     259 bp
  95% of sequence in fragments shorter than     324 bp

# DdeI.MnlI (CTNAG+CCTC) double digest, 42051 thousand fragments (14312045+27739501 sites), 70 bp average fragment length
   5% of sequence in fragments shorter than      24 bp
  10% of sequence in fragments shorter than      38 bp
  15% of sequence in fragments shorter than      52 bp
  20% of sequence in fragments shorter than      65 bp
  25% of sequence in fragments shorter than      78 bp
  30% of sequence in fragments shorter than      92 bp
  35% of sequence in fragments shorter than     106 bp
  40% of sequence in fragments shorter than     116 bp
  45% of sequence in fragments shorter than     128 bp
  50% of sequence in fragments shorter than     143 bp
  55% of sequence in fragments shorter than     160 bp
  60% of sequence in fragments shorter than     179 bp
  65% of sequence in fragments shorter than     201 bp
  70% of sequence in fragments shorter than     226 bp
  75% of sequence in fragments shorter than     256 bp
  80% of sequence in fragments shorter than     291 bp
  85% of sequence in fragments shorter than     339 bp
  90% of sequence in fragments shorter than     404 bp
  95% of sequence in fragments shorter than     526 bp

# CviAII.MnlI (CATG+CCTC) double digest, 41554 thousand fragments (13815697+27739501 sites), 71 bp average fragment length
   5% of sequence in fragments shorter than      23 bp
  10% of sequence in fragments shorter than      35 bp
  15% of sequence in fragments shorter than      47 bp
  20% of sequence in fragments shorter than      58 bp
  25% of sequence in fragments shorter than      71 bp
  30% of sequence in fragments shorter than      82 bp
  35% of sequence in fragments shorter than      94 bp
  40% of sequence in fragments shorter than     107 bp
  45% of sequence in fragments shorter than     120 bp
  50% of sequence in fragments shorter than     134 bp
  55% of sequence in fragments shorter than     150 bp
  60% of sequence in fragments shorter than     167 bp
  65% of sequence in fragments shorter than     185 bp
  70% of sequence in fragments shorter than     208 bp
  75% of sequence in fragments shorter than     235 bp
  80% of sequence in fragments shorter than     266 bp
  85% of sequence in fragments shorter than     307 bp
  90% of sequence in fragments shorter than     363 bp
  95% of sequence in fragments shorter than     462 bp

# AluI.MnlI (AGCT+CCTC) double digest, 40766 thousand fragments (13027778+27739501 sites), 73 bp average fragment length
   5% of sequence in fragments shorter than      23 bp
  10% of sequence in fragments shorter than      35 bp
  15% of sequence in fragments shorter than      48 bp
  20% of sequence in fragments shorter than      61 bp
  25% of sequence in fragments shorter than      74 bp
  30% of sequence in fragments shorter than      87 bp
  35% of sequence in fragments shorter than      99 bp
  40% of sequence in fragments shorter than     111 bp
  45% of sequence in fragments shorter than     124 bp
  50% of sequence in fragments shorter than     139 bp
  55% of sequence in fragments shorter than     156 bp
  60% of sequence in fragments shorter than     174 bp
  65% of sequence in fragments shorter than     195 bp
  70% of sequence in fragments shorter than     219 bp
  75% of sequence in fragments shorter than     248 bp
  80% of sequence in fragments shorter than     281 bp
  85% of sequence in fragments shorter than     327 bp
  90% of sequence in fragments shorter than     385 bp
  95% of sequence in fragments shorter than     492 bp

# MnlI.ScrFI (CCTC+CCNGG) double digest, 39544 thousand fragments (27739501+11805099 sites), 75 bp average fragment length
   5% of sequence in fragments shorter than      22 bp
  10% of sequence in fragments shorter than      37 bp
  15% of sequence in fragments shorter than      47 bp
  20% of sequence in fragments shorter than      61 bp
  25% of sequence in fragments shorter than      76 bp
  30% of sequence in fragments shorter than      90 bp
  35% of sequence in fragments shorter than     106 bp
  40% of sequence in fragments shorter than     123 bp
  45% of sequence in fragments shorter than     143 bp
  50% of sequence in fragments shorter than     165 bp
  55% of sequence in fragments shorter than     187 bp
  60% of sequence in fragments shorter than     215 bp
  65% of sequence in fragments shorter than     246 bp
  70% of sequence in fragments shorter than     279 bp
  75% of sequence in fragments shorter than     321 bp
  80% of sequence in fragments shorter than     367 bp
  85% of sequence in fragments shorter than     431 bp
  90% of sequence in fragments shorter than     525 bp
  95% of sequence in fragments shorter than     690 bp

# BstNI.MnlI (CCWGG+CCTC) double digest, 37594 thousand fragments (9855646+27739501 sites), 79 bp average fragment length
   5% of sequence in fragments shorter than      23 bp
  10% of sequence in fragments shorter than      39 bp
  15% of sequence in fragments shorter than      49 bp
  20% of sequence in fragments shorter than      65 bp
  25% of sequence in fragments shorter than      80 bp
  30% of sequence in fragments shorter than      94 bp
  35% of sequence in fragments shorter than     110 bp
  40% of sequence in fragments shorter than     127 bp
  45% of sequence in fragments shorter than     147 bp
  50% of sequence in fragments shorter than     168 bp
  55% of sequence in fragments shorter than     191 bp
  60% of sequence in fragments shorter than     219 bp
  65% of sequence in fragments shorter than     250 bp
  70% of sequence in fragments shorter than     283 bp
  75% of sequence in fragments shorter than     324 bp
  80% of sequence in fragments shorter than     371 bp
  85% of sequence in fragments shorter than     435 bp
  90% of sequence in fragments shorter than     530 bp
  95% of sequence in fragments shorter than     693 bp

# MboII.MnlI (GAAGA+CCTC) double digest, 36938 thousand fragments (9199496+27739501 sites), 80 bp average fragment length
   5% of sequence in fragments shorter than      26 bp
  10% of sequence in fragments shorter than      42 bp
  15% of sequence in fragments shorter than      57 bp
  20% of sequence in fragments shorter than      72 bp
  25% of sequence in fragments shorter than      86 bp
  30% of sequence in fragments shorter than     100 bp
  35% of sequence in fragments shorter than     113 bp
  40% of sequence in fragments shorter than     125 bp
  45% of sequence in fragments shorter than     138 bp
  50% of sequence in fragments shorter than     152 bp
  55% of sequence in fragments shorter than     170 bp
  60% of sequence in fragments shorter than     190 bp
  65% of sequence in fragments shorter than     212 bp
  70% of sequence in fragments shorter than     238 bp
  75% of sequence in fragments shorter than     267 bp
  80% of sequence in fragments shorter than     304 bp
  85% of sequence in fragments shorter than     350 bp
  90% of sequence in fragments shorter than     415 bp
  95% of sequence in fragments shorter than     525 bp

# Hpy188I.MnlI (TCNGA+CCTC) double digest, 36681 thousand fragments (8942153+27739501 sites), 81 bp average fragment length
   5% of sequence in fragments shorter than      26 bp
  10% of sequence in fragments shorter than      42 bp
  15% of sequence in fragments shorter than      57 bp
  20% of sequence in fragments shorter than      71 bp
  25% of sequence in fragments shorter than      86 bp
  30% of sequence in fragments shorter than     100 bp
  35% of sequence in fragments shorter than     113 bp
  40% of sequence in fragments shorter than     124 bp
  45% of sequence in fragments shorter than     138 bp
  50% of sequence in fragments shorter than     154 bp
  55% of sequence in fragments shorter than     172 bp
  60% of sequence in fragments shorter than     192 bp
  65% of sequence in fragments shorter than     215 bp
  70% of sequence in fragments shorter than     240 bp
  75% of sequence in fragments shorter than     271 bp
  80% of sequence in fragments shorter than     309 bp
  85% of sequence in fragments shorter than     356 bp
  90% of sequence in fragments shorter than     420 bp
  95% of sequence in fragments shorter than     533 bp

# HinfI.MnlI (GANTC+CCTC) double digest, 36655 thousand fragments (8916054+27739501 sites), 81 bp average fragment length
   5% of sequence in fragments shorter than      25 bp
  10% of sequence in fragments shorter than      42 bp
  15% of sequence in fragments shorter than      56 bp
  20% of sequence in fragments shorter than      69 bp
  25% of sequence in fragments shorter than      83 bp
  30% of sequence in fragments shorter than      97 bp
  35% of sequence in fragments shorter than     112 bp
  40% of sequence in fragments shorter than     123 bp
  45% of sequence in fragments shorter than     136 bp
  50% of sequence in fragments shorter than     152 bp
  55% of sequence in fragments shorter than     172 bp
  60% of sequence in fragments shorter than     193 bp
  65% of sequence in fragments shorter than     217 bp
  70% of sequence in fragments shorter than     243 bp
  75% of sequence in fragments shorter than     275 bp
  80% of sequence in fragments shorter than     315 bp
  85% of sequence in fragments shorter than     364 bp
  90% of sequence in fragments shorter than     431 bp
  95% of sequence in fragments shorter than     555 bp

# MnlI.TspRI (CCTC+CASTG) double digest, 36504 thousand fragments (27739501+8765240 sites), 81 bp average fragment length
   5% of sequence in fragments shorter than      25 bp
  10% of sequence in fragments shorter than      39 bp
  15% of sequence in fragments shorter than      55 bp
  20% of sequence in fragments shorter than      70 bp
  25% of sequence in fragments shorter than      85 bp
  30% of sequence in fragments shorter than     100 bp
  35% of sequence in fragments shorter than     113 bp
  40% of sequence in fragments shorter than     127 bp
  45% of sequence in fragments shorter than     142 bp
  50% of sequence in fragments shorter than     161 bp
  55% of sequence in fragments shorter than     180 bp
  60% of sequence in fragments shorter than     203 bp
  65% of sequence in fragments shorter than     230 bp
  70% of sequence in fragments shorter than     260 bp
  75% of sequence in fragments shorter than     294 bp
  80% of sequence in fragments shorter than     338 bp
  85% of sequence in fragments shorter than     389 bp
  90% of sequence in fragments shorter than     467 bp
  95% of sequence in fragments shorter than     597 bp

# HaeIII.MnlI (GGCC+CCTC) double digest, 36301 thousand fragments (8562230+27739501 sites), 82 bp average fragment length
   5% of sequence in fragments shorter than      26 bp
  10% of sequence in fragments shorter than      42 bp
  15% of sequence in fragments shorter than      57 bp
  20% of sequence in fragments shorter than      74 bp
  25% of sequence in fragments shorter than      89 bp
  30% of sequence in fragments shorter than     106 bp
  35% of sequence in fragments shorter than     121 bp
  40% of sequence in fragments shorter than     138 bp
  45% of sequence in fragments shorter than     158 bp
  50% of sequence in fragments shorter than     177 bp
  55% of sequence in fragments shorter than     201 bp
  60% of sequence in fragments shorter than     229 bp
  65% of sequence in fragments shorter than     260 bp
  70% of sequence in fragments shorter than     295 bp
  75% of sequence in fragments shorter than     337 bp
  80% of sequence in fragments shorter than     384 bp
  85% of sequence in fragments shorter than     448 bp
  90% of sequence in fragments shorter than     542 bp
  95% of sequence in fragments shorter than     700 bp

# BspNCI.MnlI (CCAGA+CCTC) double digest, 35021 thousand fragments (7282436+27739501 sites), 85 bp average fragment length
   5% of sequence in fragments shorter than      26 bp
  10% of sequence in fragments shorter than      43 bp
  15% of sequence in fragments shorter than      59 bp
  20% of sequence in fragments shorter than      74 bp
  25% of sequence in fragments shorter than      90 bp
  30% of sequence in fragments shorter than     105 bp
  35% of sequence in fragments shorter than     118 bp
  40% of sequence in fragments shorter than     132 bp
  45% of sequence in fragments shorter than     148 bp
  50% of sequence in fragments shorter than     167 bp
  55% of sequence in fragments shorter than     188 bp
  60% of sequence in fragments shorter than     211 bp
  65% of sequence in fragments shorter than     238 bp
  70% of sequence in fragments shorter than     267 bp
  75% of sequence in fragments shorter than     304 bp
  80% of sequence in fragments shorter than     347 bp
  85% of sequence in fragments shorter than     400 bp
  90% of sequence in fragments shorter than     475 bp
  95% of sequence in fragments shorter than     615 bp

# BspKT6I.MnlI (GATC+CCTC) double digest, 34938 thousand fragments (7199434+27739501 sites), 85 bp average fragment length
   5% of sequence in fragments shorter than      26 bp
  10% of sequence in fragments shorter than      44 bp
  15% of sequence in fragments shorter than      60 bp
  20% of sequence in fragments shorter than      76 bp
  25% of sequence in fragments shorter than      92 bp
  30% of sequence in fragments shorter than     106 bp
  35% of sequence in fragments shorter than     118 bp
  40% of sequence in fragments shorter than     133 bp
  45% of sequence in fragments shorter than     150 bp
  50% of sequence in fragments shorter than     168 bp
  55% of sequence in fragments shorter than     188 bp
  60% of sequence in fragments shorter than     211 bp
  65% of sequence in fragments shorter than     238 bp
  70% of sequence in fragments shorter than     266 bp
  75% of sequence in fragments shorter than     301 bp
  80% of sequence in fragments shorter than     342 bp
  85% of sequence in fragments shorter than     393 bp
  90% of sequence in fragments shorter than     468 bp
  95% of sequence in fragments shorter than     602 bp

# Hin4II.MnlI (CCTTC+CCTC) double digest, 34798 thousand fragments (7059915+27739501 sites), 85 bp average fragment length
   5% of sequence in fragments shorter than      26 bp
  10% of sequence in fragments shorter than      43 bp
  15% of sequence in fragments shorter than      60 bp
  20% of sequence in fragments shorter than      76 bp
  25% of sequence in fragments shorter than      92 bp
  30% of sequence in fragments shorter than     108 bp
  35% of sequence in fragments shorter than     120 bp
  40% of sequence in fragments shorter than     135 bp
  45% of sequence in fragments shorter than     151 bp
  50% of sequence in fragments shorter than     170 bp
  55% of sequence in fragments shorter than     191 bp
  60% of sequence in fragments shorter than     216 bp
  65% of sequence in fragments shorter than     244 bp
  70% of sequence in fragments shorter than     273 bp
  75% of sequence in fragments shorter than     311 bp
  80% of sequence in fragments shorter than     356 bp
  85% of sequence in fragments shorter than     410 bp
  90% of sequence in fragments shorter than     493 bp
  95% of sequence in fragments shorter than     636 bp

# BsmAI.MnlI (GTCTC+CCTC) double digest, 34370 thousand fragments (6631585+27739501 sites), 86 bp average fragment length
   5% of sequence in fragments shorter than      27 bp
  10% of sequence in fragments shorter than      43 bp
  15% of sequence in fragments shorter than      58 bp
  20% of sequence in fragments shorter than      70 bp
  25% of sequence in fragments shorter than      86 bp
  30% of sequence in fragments shorter than     101 bp
  35% of sequence in fragments shorter than     117 bp
  40% of sequence in fragments shorter than     135 bp
  45% of sequence in fragments shorter than     155 bp
  50% of sequence in fragments shorter than     174 bp
  55% of sequence in fragments shorter than     198 bp
  60% of sequence in fragments shorter than     224 bp
  65% of sequence in fragments shorter than     253 bp
  70% of sequence in fragments shorter than     287 bp
  75% of sequence in fragments shorter than     328 bp
  80% of sequence in fragments shorter than     374 bp
  85% of sequence in fragments shorter than     435 bp
  90% of sequence in fragments shorter than     526 bp
  95% of sequence in fragments shorter than     686 bp

# ApoI.MnlI (RAATTY+CCTC) double digest, 34121 thousand fragments (6382433+27739501 sites), 87 bp average fragment length
   5% of sequence in fragments shorter than      27 bp
  10% of sequence in fragments shorter than      45 bp
  15% of sequence in fragments shorter than      61 bp
  20% of sequence in fragments shorter than      76 bp
  25% of sequence in fragments shorter than      90 bp
  30% of sequence in fragments shorter than     104 bp
  35% of sequence in fragments shorter than     116 bp
  40% of sequence in fragments shorter than     129 bp
  45% of sequence in fragments shorter than     143 bp
  50% of sequence in fragments shorter than     159 bp
  55% of sequence in fragments shorter than     175 bp
  60% of sequence in fragments shorter than     196 bp
  65% of sequence in fragments shorter than     218 bp
  70% of sequence in fragments shorter than     242 bp
  75% of sequence in fragments shorter than     270 bp
  80% of sequence in fragments shorter than     305 bp
  85% of sequence in fragments shorter than     350 bp
  90% of sequence in fragments shorter than     410 bp
  95% of sequence in fragments shorter than     509 bp

# BthCI.MnlI (GCNGC+CCTC) double digest, 33948 thousand fragments (6209929+27739501 sites), 87 bp average fragment length
   5% of sequence in fragments shorter than      26 bp
  10% of sequence in fragments shorter than      44 bp
  15% of sequence in fragments shorter than      61 bp
  20% of sequence in fragments shorter than      78 bp
  25% of sequence in fragments shorter than      95 bp
  30% of sequence in fragments shorter than     112 bp
  35% of sequence in fragments shorter than     125 bp
  40% of sequence in fragments shorter than     140 bp
  45% of sequence in fragments shorter than     160 bp
  50% of sequence in fragments shorter than     180 bp
  55% of sequence in fragments shorter than     204 bp
  60% of sequence in fragments shorter than     231 bp
  65% of sequence in fragments shorter than     262 bp
  70% of sequence in fragments shorter than     296 bp
  75% of sequence in fragments shorter than     337 bp
  80% of sequence in fragments shorter than     385 bp
  85% of sequence in fragments shorter than     453 bp
  90% of sequence in fragments shorter than     545 bp
  95% of sequence in fragments shorter than     704 bp

# BccI.MnlI (CCATC+CCTC) double digest, 33909 thousand fragments (6170927+27739501 sites), 87 bp average fragment length
   5% of sequence in fragments shorter than      27 bp
  10% of sequence in fragments shorter than      44 bp
  15% of sequence in fragments shorter than      61 bp
  20% of sequence in fragments shorter than      74 bp
  25% of sequence in fragments shorter than      90 bp
  30% of sequence in fragments shorter than     105 bp
  35% of sequence in fragments shorter than     119 bp
  40% of sequence in fragments shorter than     135 bp
  45% of sequence in fragments shorter than     153 bp
  50% of sequence in fragments shorter than     173 bp
  55% of sequence in fragments shorter than     195 bp
  60% of sequence in fragments shorter than     220 bp
  65% of sequence in fragments shorter than     248 bp
  70% of sequence in fragments shorter than     279 bp
  75% of sequence in fragments shorter than     318 bp
  80% of sequence in fragments shorter than     361 bp
  85% of sequence in fragments shorter than     421 bp
  90% of sequence in fragments shorter than     506 bp
  95% of sequence in fragments shorter than     656 bp

# HphI.MnlI (GGTGA+CCTC) double digest, 33746 thousand fragments (6007336+27739501 sites), 88 bp average fragment length
   5% of sequence in fragments shorter than      28 bp
  10% of sequence in fragments shorter than      45 bp
  15% of sequence in fragments shorter than      62 bp
  20% of sequence in fragments shorter than      77 bp
  25% of sequence in fragments shorter than      92 bp
  30% of sequence in fragments shorter than     109 bp
  35% of sequence in fragments shorter than     124 bp
  40% of sequence in fragments shorter than     141 bp
  45% of sequence in fragments shorter than     161 bp
  50% of sequence in fragments shorter than     180 bp
  55% of sequence in fragments shorter than     204 bp
  60% of sequence in fragments shorter than     231 bp
  65% of sequence in fragments shorter than     260 bp
  70% of sequence in fragments shorter than     293 bp
  75% of sequence in fragments shorter than     333 bp
  80% of sequence in fragments shorter than     378 bp
  85% of sequence in fragments shorter than     438 bp
  90% of sequence in fragments shorter than     525 bp
  95% of sequence in fragments shorter than     682 bp

# DdeI.MseI (CTNAG+TTAA) double digest, 33526 thousand fragments (14312045+19214674 sites), 88 bp average fragment length
   5% of sequence in fragments shorter than      29 bp
  10% of sequence in fragments shorter than      45 bp
  15% of sequence in fragments shorter than      58 bp
  20% of sequence in fragments shorter than      71 bp
  25% of sequence in fragments shorter than      83 bp
  30% of sequence in fragments shorter than      96 bp
  35% of sequence in fragments shorter than     109 bp
  40% of sequence in fragments shorter than     121 bp
  45% of sequence in fragments shorter than     133 bp
  50% of sequence in fragments shorter than     147 bp
  55% of sequence in fragments shorter than     163 bp
  60% of sequence in fragments shorter than     177 bp
  65% of sequence in fragments shorter than     195 bp
  70% of sequence in fragments shorter than     214 bp
  75% of sequence in fragments shorter than     239 bp
  80% of sequence in fragments shorter than     269 bp
  85% of sequence in fragments shorter than     307 bp
  90% of sequence in fragments shorter than     357 bp
  95% of sequence in fragments shorter than     454 bp

# BsrI.MnlI (ACTGG+CCTC) double digest, 33480 thousand fragments (5741310+27739501 sites), 89 bp average fragment length
   5% of sequence in fragments shorter than      27 bp
  10% of sequence in fragments shorter than      45 bp
  15% of sequence in fragments shorter than      61 bp
  20% of sequence in fragments shorter than      77 bp
  25% of sequence in fragments shorter than      93 bp
  30% of sequence in fragments shorter than     109 bp
  35% of sequence in fragments shorter than     121 bp
  40% of sequence in fragments shorter than     136 bp
  45% of sequence in fragments shorter than     153 bp
  50% of sequence in fragments shorter than     172 bp
  55% of sequence in fragments shorter than     193 bp
  60% of sequence in fragments shorter than     218 bp
  65% of sequence in fragments shorter than     245 bp
  70% of sequence in fragments shorter than     276 bp
  75% of sequence in fragments shorter than     313 bp
  80% of sequence in fragments shorter than     356 bp
  85% of sequence in fragments shorter than     413 bp
  90% of sequence in fragments shorter than     490 bp
  95% of sequence in fragments shorter than     630 bp

# CviAII.MseI (CATG+TTAA) double digest, 33029 thousand fragments (13815697+19214674 sites), 90 bp average fragment length
   5% of sequence in fragments shorter than      29 bp
  10% of sequence in fragments shorter than      45 bp
  15% of sequence in fragments shorter than      58 bp
  20% of sequence in fragments shorter than      71 bp
  25% of sequence in fragments shorter than      84 bp
  30% of sequence in fragments shorter than      96 bp
  35% of sequence in fragments shorter than     110 bp
  40% of sequence in fragments shorter than     123 bp
  45% of sequence in fragments shorter than     138 bp
  50% of sequence in fragments shorter than     154 bp
  55% of sequence in fragments shorter than     171 bp
  60% of sequence in fragments shorter than     190 bp
  65% of sequence in fragments shorter than     212 bp
  70% of sequence in fragments shorter than     236 bp
  75% of sequence in fragments shorter than     265 bp
  80% of sequence in fragments shorter than     301 bp
  85% of sequence in fragments shorter than     347 bp
  90% of sequence in fragments shorter than     419 bp
  95% of sequence in fragments shorter than     565 bp

# BbvI.MnlI (GCAGC+CCTC) double digest, 33029 thousand fragments (5290051+27739501 sites), 90 bp average fragment length
   5% of sequence in fragments shorter than      27 bp
  10% of sequence in fragments shorter than      45 bp
  15% of sequence in fragments shorter than      63 bp
  20% of sequence in fragments shorter than      80 bp
  25% of sequence in fragments shorter than      97 bp
  30% of sequence in fragments shorter than     113 bp
  35% of sequence in fragments shorter than     127 bp
  40% of sequence in fragments shorter than     142 bp
  45% of sequence in fragments shorter than     162 bp
  50% of sequence in fragments shorter than     183 bp
  55% of sequence in fragments shorter than     207 bp
  60% of sequence in fragments shorter than     234 bp
  65% of sequence in fragments shorter than     264 bp
  70% of sequence in fragments shorter than     299 bp
  75% of sequence in fragments shorter than     340 bp
  80% of sequence in fragments shorter than     389 bp
  85% of sequence in fragments shorter than     457 bp
  90% of sequence in fragments shorter than     550 bp
  95% of sequence in fragments shorter than     709 bp

# FokI.MnlI (GGATG+CCTC) double digest, 32940 thousand fragments (5201116+27739501 sites), 90 bp average fragment length
   5% of sequence in fragments shorter than      27 bp
  10% of sequence in fragments shorter than      46 bp
  15% of sequence in fragments shorter than      65 bp
  20% of sequence in fragments shorter than      81 bp
  25% of sequence in fragments shorter than      97 bp
  30% of sequence in fragments shorter than     112 bp
  35% of sequence in fragments shorter than     126 bp
  40% of sequence in fragments shorter than     143 bp
  45% of sequence in fragments shorter than     161 bp
  50% of sequence in fragments shorter than     181 bp
  55% of sequence in fragments shorter than     204 bp
  60% of sequence in fragments shorter than     230 bp
  65% of sequence in fragments shorter than     259 bp
  70% of sequence in fragments shorter than     291 bp
  75% of sequence in fragments shorter than     331 bp
  80% of sequence in fragments shorter than     377 bp
  85% of sequence in fragments shorter than     441 bp
  90% of sequence in fragments shorter than     531 bp
  95% of sequence in fragments shorter than     688 bp

# CviQI.MnlI (GTAC+CCTC) double digest, 32854 thousand fragments (5115078+27739501 sites), 90 bp average fragment length
   5% of sequence in fragments shorter than      28 bp
  10% of sequence in fragments shorter than      46 bp
  15% of sequence in fragments shorter than      64 bp
  20% of sequence in fragments shorter than      79 bp
  25% of sequence in fragments shorter than      95 bp
  30% of sequence in fragments shorter than     110 bp
  35% of sequence in fragments shorter than     122 bp
  40% of sequence in fragments shorter than     136 bp
  45% of sequence in fragments shorter than     153 bp
  50% of sequence in fragments shorter than     171 bp
  55% of sequence in fragments shorter than     191 bp
  60% of sequence in fragments shorter than     215 bp
  65% of sequence in fragments shorter than     241 bp
  70% of sequence in fragments shorter than     269 bp
  75% of sequence in fragments shorter than     304 bp
  80% of sequence in fragments shorter than     345 bp
  85% of sequence in fragments shorter than     397 bp
  90% of sequence in fragments shorter than     469 bp
  95% of sequence in fragments shorter than     603 bp

# MnlI.TfiI (CCTC+GAWTC) double digest, 32723 thousand fragments (27739501+4984362 sites), 91 bp average fragment length
   5% of sequence in fragments shorter than      28 bp
  10% of sequence in fragments shorter than      47 bp
  15% of sequence in fragments shorter than      65 bp
  20% of sequence in fragments shorter than      81 bp
  25% of sequence in fragments shorter than      97 bp
  30% of sequence in fragments shorter than     112 bp
  35% of sequence in fragments shorter than     125 bp
  40% of sequence in fragments shorter than     139 bp
  45% of sequence in fragments shorter than     156 bp
  50% of sequence in fragments shorter than     175 bp
  55% of sequence in fragments shorter than     197 bp
  60% of sequence in fragments shorter than     221 bp
  65% of sequence in fragments shorter than     247 bp
  70% of sequence in fragments shorter than     277 bp
  75% of sequence in fragments shorter than     314 bp
  80% of sequence in fragments shorter than     357 bp
  85% of sequence in fragments shorter than     412 bp
  90% of sequence in fragments shorter than     490 bp
  95% of sequence in fragments shorter than     628 bp

# AluI.MseI (AGCT+TTAA) double digest, 32241 thousand fragments (13027778+19214674 sites), 92 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      47 bp
  15% of sequence in fragments shorter than      61 bp
  20% of sequence in fragments shorter than      74 bp
  25% of sequence in fragments shorter than      87 bp
  30% of sequence in fragments shorter than     101 bp
  35% of sequence in fragments shorter than     115 bp
  40% of sequence in fragments shorter than     129 bp
  45% of sequence in fragments shorter than     144 bp
  50% of sequence in fragments shorter than     159 bp
  55% of sequence in fragments shorter than     176 bp
  60% of sequence in fragments shorter than     193 bp
  65% of sequence in fragments shorter than     212 bp
  70% of sequence in fragments shorter than     235 bp
  75% of sequence in fragments shorter than     262 bp
  80% of sequence in fragments shorter than     293 bp
  85% of sequence in fragments shorter than     332 bp
  90% of sequence in fragments shorter than     385 bp
  95% of sequence in fragments shorter than     490 bp

# AciI.MnlI (CCGC+CCTC) double digest, 31945 thousand fragments (4206130+27739501 sites), 93 bp average fragment length
   5% of sequence in fragments shorter than      28 bp
  10% of sequence in fragments shorter than      47 bp
  15% of sequence in fragments shorter than      67 bp
  20% of sequence in fragments shorter than      86 bp
  25% of sequence in fragments shorter than     104 bp
  30% of sequence in fragments shorter than     119 bp
  35% of sequence in fragments shorter than     136 bp
  40% of sequence in fragments shorter than     156 bp
  45% of sequence in fragments shorter than     177 bp
  50% of sequence in fragments shorter than     202 bp
  55% of sequence in fragments shorter than     230 bp
  60% of sequence in fragments shorter than     260 bp
  65% of sequence in fragments shorter than     293 bp
  70% of sequence in fragments shorter than     332 bp
  75% of sequence in fragments shorter than     374 bp
  80% of sequence in fragments shorter than     431 bp
  85% of sequence in fragments shorter than     504 bp
  90% of sequence in fragments shorter than     610 bp
  95% of sequence in fragments shorter than     783 bp

# MnlI.Tsp45I (CCTC+GTSAC) double digest, 31760 thousand fragments (27739501+4021761 sites), 93 bp average fragment length
   5% of sequence in fragments shorter than      29 bp
  10% of sequence in fragments shorter than      47 bp
  15% of sequence in fragments shorter than      65 bp
  20% of sequence in fragments shorter than      82 bp
  25% of sequence in fragments shorter than      98 bp
  30% of sequence in fragments shorter than     113 bp
  35% of sequence in fragments shorter than     128 bp
  40% of sequence in fragments shorter than     144 bp
  45% of sequence in fragments shorter than     164 bp
  50% of sequence in fragments shorter than     184 bp
  55% of sequence in fragments shorter than     208 bp
  60% of sequence in fragments shorter than     235 bp
  65% of sequence in fragments shorter than     264 bp
  70% of sequence in fragments shorter than     298 bp
  75% of sequence in fragments shorter than     339 bp
  80% of sequence in fragments shorter than     384 bp
  85% of sequence in fragments shorter than     446 bp
  90% of sequence in fragments shorter than     538 bp
  95% of sequence in fragments shorter than     694 bp

# MlyI.MnlI (GAGTC+CCTC) double digest, 31670 thousand fragments (3931692+27739501 sites), 94 bp average fragment length
   5% of sequence in fragments shorter than      29 bp
  10% of sequence in fragments shorter than      48 bp
  15% of sequence in fragments shorter than      64 bp
  20% of sequence in fragments shorter than      81 bp
  25% of sequence in fragments shorter than      98 bp
  30% of sequence in fragments shorter than     113 bp
  35% of sequence in fragments shorter than     128 bp
  40% of sequence in fragments shorter than     144 bp
  45% of sequence in fragments shorter than     165 bp
  50% of sequence in fragments shorter than     185 bp
  55% of sequence in fragments shorter than     211 bp
  60% of sequence in fragments shorter than     238 bp
  65% of sequence in fragments shorter than     267 bp
  70% of sequence in fragments shorter than     303 bp
  75% of sequence in fragments shorter than     345 bp
  80% of sequence in fragments shorter than     392 bp
  85% of sequence in fragments shorter than     456 bp
  90% of sequence in fragments shorter than     550 bp
  95% of sequence in fragments shorter than     703 bp

# BscAI.MnlI (GCATC+CCTC) double digest, 31391 thousand fragments (3652926+27739501 sites), 94 bp average fragment length
   5% of sequence in fragments shorter than      29 bp
  10% of sequence in fragments shorter than      48 bp
  15% of sequence in fragments shorter than      67 bp
  20% of sequence in fragments shorter than      85 bp
  25% of sequence in fragments shorter than     101 bp
  30% of sequence in fragments shorter than     115 bp
  35% of sequence in fragments shorter than     130 bp
  40% of sequence in fragments shorter than     147 bp
  45% of sequence in fragments shorter than     166 bp
  50% of sequence in fragments shorter than     186 bp
  55% of sequence in fragments shorter than     210 bp
  60% of sequence in fragments shorter than     237 bp
  65% of sequence in fragments shorter than     265 bp
  70% of sequence in fragments shorter than     299 bp
  75% of sequence in fragments shorter than     340 bp
  80% of sequence in fragments shorter than     386 bp
  85% of sequence in fragments shorter than     448 bp
  90% of sequence in fragments shorter than     537 bp
  95% of sequence in fragments shorter than     690 bp

# MnlI.SfeI (CCTC+CTRYAG) double digest, 31270 thousand fragments (27739501+3531009 sites), 95 bp average fragment length
   5% of sequence in fragments shorter than      28 bp
  10% of sequence in fragments shorter than      48 bp
  15% of sequence in fragments shorter than      67 bp
  20% of sequence in fragments shorter than      85 bp
  25% of sequence in fragments shorter than     101 bp
  30% of sequence in fragments shorter than     115 bp
  35% of sequence in fragments shorter than     131 bp
  40% of sequence in fragments shorter than     149 bp
  45% of sequence in fragments shorter than     169 bp
  50% of sequence in fragments shorter than     191 bp
  55% of sequence in fragments shorter than     216 bp
  60% of sequence in fragments shorter than     243 bp
  65% of sequence in fragments shorter than     272 bp
  70% of sequence in fragments shorter than     307 bp
  75% of sequence in fragments shorter than     346 bp
  80% of sequence in fragments shorter than     392 bp
  85% of sequence in fragments shorter than     453 bp
  90% of sequence in fragments shorter than     543 bp
  95% of sequence in fragments shorter than     695 bp

# MseI.ScrFI (TTAA+CCNGG) double digest, 31019 thousand fragments (19214674+11805099 sites), 96 bp average fragment length
   5% of sequence in fragments shorter than      33 bp
  10% of sequence in fragments shorter than      50 bp
  15% of sequence in fragments shorter than      62 bp
  20% of sequence in fragments shorter than      75 bp
  25% of sequence in fragments shorter than      87 bp
  30% of sequence in fragments shorter than     101 bp
  35% of sequence in fragments shorter than     115 bp
  40% of sequence in fragments shorter than     127 bp
  45% of sequence in fragments shorter than     142 bp
  50% of sequence in fragments shorter than     158 bp
  55% of sequence in fragments shorter than     175 bp
  60% of sequence in fragments shorter than     194 bp
  65% of sequence in fragments shorter than     215 bp
  70% of sequence in fragments shorter than     239 bp
  75% of sequence in fragments shorter than     268 bp
  80% of sequence in fragments shorter than     301 bp
  85% of sequence in fragments shorter than     344 bp
  90% of sequence in fragments shorter than     406 bp
  95% of sequence in fragments shorter than     532 bp

# MnlI.StyI (CCTC+CCWWGG) double digest, 30853 thousand fragments (27739501+3114107 sites), 96 bp average fragment length
   5% of sequence in fragments shorter than      29 bp
  10% of sequence in fragments shorter than      49 bp
  15% of sequence in fragments shorter than      68 bp
  20% of sequence in fragments shorter than      87 bp
  25% of sequence in fragments shorter than     104 bp
  30% of sequence in fragments shorter than     118 bp
  35% of sequence in fragments shorter than     133 bp
  40% of sequence in fragments shorter than     152 bp
  45% of sequence in fragments shorter than     172 bp
  50% of sequence in fragments shorter than     194 bp
  55% of sequence in fragments shorter than     219 bp
  60% of sequence in fragments shorter than     247 bp
  65% of sequence in fragments shorter than     278 bp
  70% of sequence in fragments shorter than     315 bp
  75% of sequence in fragments shorter than     356 bp
  80% of sequence in fragments shorter than     408 bp
  85% of sequence in fragments shorter than     472 bp
  90% of sequence in fragments shorter than     569 bp
  95% of sequence in fragments shorter than     736 bp

# MnlI.NspI (CCTC+RCATGY) double digest, 30843 thousand fragments (27739501+3104359 sites), 96 bp average fragment length
   5% of sequence in fragments shorter than      29 bp
  10% of sequence in fragments shorter than      49 bp
  15% of sequence in fragments shorter than      68 bp
  20% of sequence in fragments shorter than      86 bp
  25% of sequence in fragments shorter than     101 bp
  30% of sequence in fragments shorter than     115 bp
  35% of sequence in fragments shorter than     131 bp
  40% of sequence in fragments shorter than     148 bp
  45% of sequence in fragments shorter than     168 bp
  50% of sequence in fragments shorter than     188 bp
  55% of sequence in fragments shorter than     212 bp
  60% of sequence in fragments shorter than     238 bp
  65% of sequence in fragments shorter than     266 bp
  70% of sequence in fragments shorter than     300 bp
  75% of sequence in fragments shorter than     339 bp
  80% of sequence in fragments shorter than     383 bp
  85% of sequence in fragments shorter than     447 bp
  90% of sequence in fragments shorter than     534 bp
  95% of sequence in fragments shorter than     688 bp

# AvaII.MnlI (GGWCC+CCTC) double digest, 30486 thousand fragments (2747690+27739501 sites), 97 bp average fragment length
   5% of sequence in fragments shorter than      30 bp
  10% of sequence in fragments shorter than      50 bp
  15% of sequence in fragments shorter than      70 bp
  20% of sequence in fragments shorter than      89 bp
  25% of sequence in fragments shorter than     107 bp
  30% of sequence in fragments shorter than     120 bp
  35% of sequence in fragments shorter than     137 bp
  40% of sequence in fragments shorter than     157 bp
  45% of sequence in fragments shorter than     177 bp
  50% of sequence in fragments shorter than     201 bp
  55% of sequence in fragments shorter than     228 bp
  60% of sequence in fragments shorter than     257 bp
  65% of sequence in fragments shorter than     290 bp
  70% of sequence in fragments shorter than     330 bp
  75% of sequence in fragments shorter than     370 bp
  80% of sequence in fragments shorter than     424 bp
  85% of sequence in fragments shorter than     495 bp
  90% of sequence in fragments shorter than     598 bp
  95% of sequence in fragments shorter than     765 bp

# MnlI.SmlI (CCTC+CTYRAG) double digest, 30466 thousand fragments (27739501+2727867 sites), 97 bp average fragment length
   5% of sequence in fragments shorter than      30 bp
  10% of sequence in fragments shorter than      52 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      90 bp
  25% of sequence in fragments shorter than     108 bp
  30% of sequence in fragments shorter than     120 bp
  35% of sequence in fragments shorter than     137 bp
  40% of sequence in fragments shorter than     156 bp
  45% of sequence in fragments shorter than     176 bp
  50% of sequence in fragments shorter than     199 bp
  55% of sequence in fragments shorter than     226 bp
  60% of sequence in fragments shorter than     253 bp
  65% of sequence in fragments shorter than     285 bp
  70% of sequence in fragments shorter than     323 bp
  75% of sequence in fragments shorter than     363 bp
  80% of sequence in fragments shorter than     414 bp
  85% of sequence in fragments shorter than     479 bp
  90% of sequence in fragments shorter than     575 bp
  95% of sequence in fragments shorter than     732 bp

# MnlI.SspI (CCTC+AATATT) double digest, 30116 thousand fragments (27739501+2377267 sites), 98 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      52 bp
  15% of sequence in fragments shorter than      71 bp
  20% of sequence in fragments shorter than      89 bp
  25% of sequence in fragments shorter than     106 bp
  30% of sequence in fragments shorter than     119 bp
  35% of sequence in fragments shorter than     134 bp
  40% of sequence in fragments shorter than     150 bp
  45% of sequence in fragments shorter than     169 bp
  50% of sequence in fragments shorter than     188 bp
  55% of sequence in fragments shorter than     211 bp
  60% of sequence in fragments shorter than     236 bp
  65% of sequence in fragments shorter than     263 bp
  70% of sequence in fragments shorter than     295 bp
  75% of sequence in fragments shorter than     332 bp
  80% of sequence in fragments shorter than     372 bp
  85% of sequence in fragments shorter than     427 bp
  90% of sequence in fragments shorter than     506 bp
  95% of sequence in fragments shorter than     646 bp

# HpaII.MnlI (CCGG+CCTC) double digest, 30056 thousand fragments (2317722+27739501 sites), 99 bp average fragment length
   5% of sequence in fragments shorter than      30 bp
  10% of sequence in fragments shorter than      49 bp
  15% of sequence in fragments shorter than      70 bp
  20% of sequence in fragments shorter than      89 bp
  25% of sequence in fragments shorter than     108 bp
  30% of sequence in fragments shorter than     123 bp
  35% of sequence in fragments shorter than     141 bp
  40% of sequence in fragments shorter than     162 bp
  45% of sequence in fragments shorter than     184 bp
  50% of sequence in fragments shorter than     210 bp
  55% of sequence in fragments shorter than     238 bp
  60% of sequence in fragments shorter than     268 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     344 bp
  75% of sequence in fragments shorter than     387 bp
  80% of sequence in fragments shorter than     443 bp
  85% of sequence in fragments shorter than     517 bp
  90% of sequence in fragments shorter than     627 bp
  95% of sequence in fragments shorter than     801 bp

# HhaI.MnlI (GCGC+CCTC) double digest, 29495 thousand fragments (1756502+27739501 sites), 101 bp average fragment length
   5% of sequence in fragments shorter than      30 bp
  10% of sequence in fragments shorter than      51 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      90 bp
  25% of sequence in fragments shorter than     109 bp
  30% of sequence in fragments shorter than     124 bp
  35% of sequence in fragments shorter than     142 bp
  40% of sequence in fragments shorter than     164 bp
  45% of sequence in fragments shorter than     184 bp
  50% of sequence in fragments shorter than     210 bp
  55% of sequence in fragments shorter than     239 bp
  60% of sequence in fragments shorter than     269 bp
  65% of sequence in fragments shorter than     304 bp
  70% of sequence in fragments shorter than     345 bp
  75% of sequence in fragments shorter than     389 bp
  80% of sequence in fragments shorter than     445 bp
  85% of sequence in fragments shorter than     520 bp
  90% of sequence in fragments shorter than     630 bp
  95% of sequence in fragments shorter than     806 bp

# BfiI.MnlI (ACTGGG+CCTC) double digest, 29445 thousand fragments (1706594+27739501 sites), 101 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      52 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      91 bp
  25% of sequence in fragments shorter than     109 bp
  30% of sequence in fragments shorter than     123 bp
  35% of sequence in fragments shorter than     139 bp
  40% of sequence in fragments shorter than     159 bp
  45% of sequence in fragments shorter than     180 bp
  50% of sequence in fragments shorter than     205 bp
  55% of sequence in fragments shorter than     232 bp
  60% of sequence in fragments shorter than     262 bp
  65% of sequence in fragments shorter than     295 bp
  70% of sequence in fragments shorter than     335 bp
  75% of sequence in fragments shorter than     374 bp
  80% of sequence in fragments shorter than     430 bp
  85% of sequence in fragments shorter than     502 bp
  90% of sequence in fragments shorter than     608 bp
  95% of sequence in fragments shorter than     777 bp

# MnlI.PsiI (CCTC+TTATAA) double digest, 29386 thousand fragments (27739501+1647912 sites), 101 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      91 bp
  25% of sequence in fragments shorter than     109 bp
  30% of sequence in fragments shorter than     122 bp
  35% of sequence in fragments shorter than     138 bp
  40% of sequence in fragments shorter than     156 bp
  45% of sequence in fragments shorter than     175 bp
  50% of sequence in fragments shorter than     198 bp
  55% of sequence in fragments shorter than     223 bp
  60% of sequence in fragments shorter than     250 bp
  65% of sequence in fragments shorter than     279 bp
  70% of sequence in fragments shorter than     313 bp
  75% of sequence in fragments shorter than     351 bp
  80% of sequence in fragments shorter than     398 bp
  85% of sequence in fragments shorter than     459 bp
  90% of sequence in fragments shorter than     546 bp
  95% of sequence in fragments shorter than     694 bp

# EsaBC3I.MnlI (TCGA+CCTC) double digest, 29342 thousand fragments (1603346+27739501 sites), 101 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      73 bp
  20% of sequence in fragments shorter than      93 bp
  25% of sequence in fragments shorter than     109 bp
  30% of sequence in fragments shorter than     124 bp
  35% of sequence in fragments shorter than     141 bp
  40% of sequence in fragments shorter than     162 bp
  45% of sequence in fragments shorter than     183 bp
  50% of sequence in fragments shorter than     208 bp
  55% of sequence in fragments shorter than     235 bp
  60% of sequence in fragments shorter than     264 bp
  65% of sequence in fragments shorter than     297 bp
  70% of sequence in fragments shorter than     336 bp
  75% of sequence in fragments shorter than     378 bp
  80% of sequence in fragments shorter than     431 bp
  85% of sequence in fragments shorter than     503 bp
  90% of sequence in fragments shorter than     606 bp
  95% of sequence in fragments shorter than     772 bp

# HgiCI.MnlI (GGYRCC+CCTC) double digest, 29289 thousand fragments (1550877+27739501 sites), 101 bp average fragment length
   5% of sequence in fragments shorter than      30 bp
  10% of sequence in fragments shorter than      52 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      91 bp
  25% of sequence in fragments shorter than     109 bp
  30% of sequence in fragments shorter than     125 bp
  35% of sequence in fragments shorter than     143 bp
  40% of sequence in fragments shorter than     164 bp
  45% of sequence in fragments shorter than     185 bp
  50% of sequence in fragments shorter than     211 bp
  55% of sequence in fragments shorter than     240 bp
  60% of sequence in fragments shorter than     269 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     344 bp
  75% of sequence in fragments shorter than     386 bp
  80% of sequence in fragments shorter than     442 bp
  85% of sequence in fragments shorter than     516 bp
  90% of sequence in fragments shorter than     625 bp
  95% of sequence in fragments shorter than     796 bp

# BsmI.MnlI (GAATGC+CCTC) double digest, 29288 thousand fragments (1549350+27739501 sites), 101 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      52 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      91 bp
  25% of sequence in fragments shorter than     109 bp
  30% of sequence in fragments shorter than     122 bp
  35% of sequence in fragments shorter than     138 bp
  40% of sequence in fragments shorter than     157 bp
  45% of sequence in fragments shorter than     178 bp
  50% of sequence in fragments shorter than     202 bp
  55% of sequence in fragments shorter than     228 bp
  60% of sequence in fragments shorter than     258 bp
  65% of sequence in fragments shorter than     290 bp
  70% of sequence in fragments shorter than     328 bp
  75% of sequence in fragments shorter than     371 bp
  80% of sequence in fragments shorter than     424 bp
  85% of sequence in fragments shorter than     494 bp
  90% of sequence in fragments shorter than     596 bp
  95% of sequence in fragments shorter than     761 bp

# AflIII.MnlI (ACRYGT+CCTC) double digest, 29224 thousand fragments (1485394+27739501 sites), 101 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      73 bp
  20% of sequence in fragments shorter than      92 bp
  25% of sequence in fragments shorter than     109 bp
  30% of sequence in fragments shorter than     122 bp
  35% of sequence in fragments shorter than     139 bp
  40% of sequence in fragments shorter than     158 bp
  45% of sequence in fragments shorter than     178 bp
  50% of sequence in fragments shorter than     202 bp
  55% of sequence in fragments shorter than     228 bp
  60% of sequence in fragments shorter than     256 bp
  65% of sequence in fragments shorter than     287 bp
  70% of sequence in fragments shorter than     323 bp
  75% of sequence in fragments shorter than     363 bp
  80% of sequence in fragments shorter than     415 bp
  85% of sequence in fragments shorter than     483 bp
  90% of sequence in fragments shorter than     580 bp
  95% of sequence in fragments shorter than     738 bp

# FauI.MnlI (CCCGC+CCTC) double digest, 29178 thousand fragments (1439772+27739501 sites), 102 bp average fragment length
   5% of sequence in fragments shorter than      30 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      73 bp
  20% of sequence in fragments shorter than      93 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     145 bp
  40% of sequence in fragments shorter than     166 bp
  45% of sequence in fragments shorter than     188 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     244 bp
  60% of sequence in fragments shorter than     274 bp
  65% of sequence in fragments shorter than     310 bp
  70% of sequence in fragments shorter than     348 bp
  75% of sequence in fragments shorter than     394 bp
  80% of sequence in fragments shorter than     451 bp
  85% of sequence in fragments shorter than     526 bp
  90% of sequence in fragments shorter than     637 bp
  95% of sequence in fragments shorter than     813 bp

# BspMI.MnlI (ACCTGC+CCTC) double digest, 29177 thousand fragments (1438129+27739501 sites), 102 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      73 bp
  20% of sequence in fragments shorter than      92 bp
  25% of sequence in fragments shorter than     111 bp
  30% of sequence in fragments shorter than     124 bp
  35% of sequence in fragments shorter than     140 bp
  40% of sequence in fragments shorter than     160 bp
  45% of sequence in fragments shorter than     181 bp
  50% of sequence in fragments shorter than     206 bp
  55% of sequence in fragments shorter than     234 bp
  60% of sequence in fragments shorter than     264 bp
  65% of sequence in fragments shorter than     298 bp
  70% of sequence in fragments shorter than     338 bp
  75% of sequence in fragments shorter than     380 bp
  80% of sequence in fragments shorter than     435 bp
  85% of sequence in fragments shorter than     508 bp
  90% of sequence in fragments shorter than     613 bp
  95% of sequence in fragments shorter than     785 bp

# BstNI.MseI (CCWGG+TTAA) double digest, 29069 thousand fragments (9855646+19214674 sites), 102 bp average fragment length
   5% of sequence in fragments shorter than      35 bp
  10% of sequence in fragments shorter than      51 bp
  15% of sequence in fragments shorter than      67 bp
  20% of sequence in fragments shorter than      81 bp
  25% of sequence in fragments shorter than      94 bp
  30% of sequence in fragments shorter than     109 bp
  35% of sequence in fragments shorter than     121 bp
  40% of sequence in fragments shorter than     136 bp
  45% of sequence in fragments shorter than     151 bp
  50% of sequence in fragments shorter than     168 bp
  55% of sequence in fragments shorter than     185 bp
  60% of sequence in fragments shorter than     205 bp
  65% of sequence in fragments shorter than     227 bp
  70% of sequence in fragments shorter than     253 bp
  75% of sequence in fragments shorter than     281 bp
  80% of sequence in fragments shorter than     316 bp
  85% of sequence in fragments shorter than     359 bp
  90% of sequence in fragments shorter than     425 bp
  95% of sequence in fragments shorter than     557 bp

# MnlI.PstI (CCTC+CTGCAG) double digest, 29060 thousand fragments (27739501+1321469 sites), 102 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      73 bp
  20% of sequence in fragments shorter than      93 bp
  25% of sequence in fragments shorter than     111 bp
  30% of sequence in fragments shorter than     125 bp
  35% of sequence in fragments shorter than     142 bp
  40% of sequence in fragments shorter than     163 bp
  45% of sequence in fragments shorter than     184 bp
  50% of sequence in fragments shorter than     210 bp
  55% of sequence in fragments shorter than     238 bp
  60% of sequence in fragments shorter than     267 bp
  65% of sequence in fragments shorter than     302 bp
  70% of sequence in fragments shorter than     341 bp
  75% of sequence in fragments shorter than     384 bp
  80% of sequence in fragments shorter than     439 bp
  85% of sequence in fragments shorter than     513 bp
  90% of sequence in fragments shorter than     620 bp
  95% of sequence in fragments shorter than     792 bp

# BscGI.MnlI (CCCGT+CCTC) double digest, 28970 thousand fragments (1231070+27739501 sites), 102 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      52 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      91 bp
  25% of sequence in fragments shorter than     110 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     144 bp
  40% of sequence in fragments shorter than     164 bp
  45% of sequence in fragments shorter than     186 bp
  50% of sequence in fragments shorter than     212 bp
  55% of sequence in fragments shorter than     240 bp
  60% of sequence in fragments shorter than     270 bp
  65% of sequence in fragments shorter than     306 bp
  70% of sequence in fragments shorter than     344 bp
  75% of sequence in fragments shorter than     389 bp
  80% of sequence in fragments shorter than     445 bp
  85% of sequence in fragments shorter than     520 bp
  90% of sequence in fragments shorter than     630 bp
  95% of sequence in fragments shorter than     804 bp

# AccI.MnlI (GTMKAC+CCTC) double digest, 28840 thousand fragments (1101063+27739501 sites), 103 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      74 bp
  20% of sequence in fragments shorter than      93 bp
  25% of sequence in fragments shorter than     111 bp
  30% of sequence in fragments shorter than     125 bp
  35% of sequence in fragments shorter than     141 bp
  40% of sequence in fragments shorter than     161 bp
  45% of sequence in fragments shorter than     182 bp
  50% of sequence in fragments shorter than     206 bp
  55% of sequence in fragments shorter than     233 bp
  60% of sequence in fragments shorter than     261 bp
  65% of sequence in fragments shorter than     293 bp
  70% of sequence in fragments shorter than     332 bp
  75% of sequence in fragments shorter than     371 bp
  80% of sequence in fragments shorter than     424 bp
  85% of sequence in fragments shorter than     492 bp
  90% of sequence in fragments shorter than     592 bp
  95% of sequence in fragments shorter than     752 bp

# MnlI.PpuMI (CCTC+RGGWCCY) double digest, 28824 thousand fragments (27739501+1085138 sites), 103 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     145 bp
  40% of sequence in fragments shorter than     166 bp
  45% of sequence in fragments shorter than     188 bp
  50% of sequence in fragments shorter than     214 bp
  55% of sequence in fragments shorter than     242 bp
  60% of sequence in fragments shorter than     272 bp
  65% of sequence in fragments shorter than     307 bp
  70% of sequence in fragments shorter than     346 bp
  75% of sequence in fragments shorter than     391 bp
  80% of sequence in fragments shorter than     447 bp
  85% of sequence in fragments shorter than     521 bp
  90% of sequence in fragments shorter than     631 bp
  95% of sequence in fragments shorter than     804 bp

# MnlI.PvuII (CCTC+CAGCTG) double digest, 28823 thousand fragments (27739501+1084260 sites), 103 bp average fragment length
   5% of sequence in fragments shorter than      31 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      74 bp
  20% of sequence in fragments shorter than      93 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     143 bp
  40% of sequence in fragments shorter than     164 bp
  45% of sequence in fragments shorter than     185 bp
  50% of sequence in fragments shorter than     211 bp
  55% of sequence in fragments shorter than     239 bp
  60% of sequence in fragments shorter than     269 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     343 bp
  75% of sequence in fragments shorter than     386 bp
  80% of sequence in fragments shorter than     441 bp
  85% of sequence in fragments shorter than     515 bp
  90% of sequence in fragments shorter than     623 bp
  95% of sequence in fragments shorter than     796 bp

# AvaI.MnlI (CYCGRG+CCTC) double digest, 28744 thousand fragments (1005714+27739501 sites), 103 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     217 bp
  55% of sequence in fragments shorter than     247 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     312 bp
  70% of sequence in fragments shorter than     351 bp
  75% of sequence in fragments shorter than     398 bp
  80% of sequence in fragments shorter than     455 bp
  85% of sequence in fragments shorter than     530 bp
  90% of sequence in fragments shorter than     641 bp
  95% of sequence in fragments shorter than     817 bp

# BspHI.MnlI (TCATGA+CCTC) double digest, 28717 thousand fragments (978290+27739501 sites), 103 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      94 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     143 bp
  40% of sequence in fragments shorter than     164 bp
  45% of sequence in fragments shorter than     184 bp
  50% of sequence in fragments shorter than     209 bp
  55% of sequence in fragments shorter than     237 bp
  60% of sequence in fragments shorter than     266 bp
  65% of sequence in fragments shorter than     299 bp
  70% of sequence in fragments shorter than     338 bp
  75% of sequence in fragments shorter than     379 bp
  80% of sequence in fragments shorter than     432 bp
  85% of sequence in fragments shorter than     502 bp
  90% of sequence in fragments shorter than     604 bp
  95% of sequence in fragments shorter than     770 bp

# AvaIII.MnlI (ATGCAT+CCTC) double digest, 28672 thousand fragments (933357+27739501 sites), 103 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      94 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     142 bp
  40% of sequence in fragments shorter than     162 bp
  45% of sequence in fragments shorter than     183 bp
  50% of sequence in fragments shorter than     207 bp
  55% of sequence in fragments shorter than     234 bp
  60% of sequence in fragments shorter than     263 bp
  65% of sequence in fragments shorter than     295 bp
  70% of sequence in fragments shorter than     333 bp
  75% of sequence in fragments shorter than     373 bp
  80% of sequence in fragments shorter than     425 bp
  85% of sequence in fragments shorter than     494 bp
  90% of sequence in fragments shorter than     593 bp
  95% of sequence in fragments shorter than     752 bp

# MnlI.StuI (CCTC+AGGCCT) double digest, 28664 thousand fragments (27739501+925729 sites), 103 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     188 bp
  50% of sequence in fragments shorter than     214 bp
  55% of sequence in fragments shorter than     243 bp
  60% of sequence in fragments shorter than     273 bp
  65% of sequence in fragments shorter than     308 bp
  70% of sequence in fragments shorter than     346 bp
  75% of sequence in fragments shorter than     392 bp
  80% of sequence in fragments shorter than     448 bp
  85% of sequence in fragments shorter than     522 bp
  90% of sequence in fragments shorter than     632 bp
  95% of sequence in fragments shorter than     807 bp

# MnlI.NdeI (CCTC+CATATG) double digest, 28642 thousand fragments (27739501+903360 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      94 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     142 bp
  40% of sequence in fragments shorter than     162 bp
  45% of sequence in fragments shorter than     182 bp
  50% of sequence in fragments shorter than     207 bp
  55% of sequence in fragments shorter than     234 bp
  60% of sequence in fragments shorter than     262 bp
  65% of sequence in fragments shorter than     295 bp
  70% of sequence in fragments shorter than     333 bp
  75% of sequence in fragments shorter than     372 bp
  80% of sequence in fragments shorter than     425 bp
  85% of sequence in fragments shorter than     493 bp
  90% of sequence in fragments shorter than     587 bp
  95% of sequence in fragments shorter than     750 bp

# HindIII.MnlI (AAGCTT+CCTC) double digest, 28599 thousand fragments (860365+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      94 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     143 bp
  40% of sequence in fragments shorter than     163 bp
  45% of sequence in fragments shorter than     184 bp
  50% of sequence in fragments shorter than     209 bp
  55% of sequence in fragments shorter than     236 bp
  60% of sequence in fragments shorter than     265 bp
  65% of sequence in fragments shorter than     298 bp
  70% of sequence in fragments shorter than     336 bp
  75% of sequence in fragments shorter than     378 bp
  80% of sequence in fragments shorter than     430 bp
  85% of sequence in fragments shorter than     501 bp
  90% of sequence in fragments shorter than     604 bp
  95% of sequence in fragments shorter than     770 bp

# MnlI.XbaI (CCTC+TCTAGA) double digest, 28589 thousand fragments (27739501+850998 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     143 bp
  40% of sequence in fragments shorter than     164 bp
  45% of sequence in fragments shorter than     185 bp
  50% of sequence in fragments shorter than     211 bp
  55% of sequence in fragments shorter than     237 bp
  60% of sequence in fragments shorter than     266 bp
  65% of sequence in fragments shorter than     300 bp
  70% of sequence in fragments shorter than     339 bp
  75% of sequence in fragments shorter than     381 bp
  80% of sequence in fragments shorter than     434 bp
  85% of sequence in fragments shorter than     506 bp
  90% of sequence in fragments shorter than     610 bp
  95% of sequence in fragments shorter than     777 bp

# EcoRI.MnlI (GAATTC+CCTC) double digest, 28586 thousand fragments (847401+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      94 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     143 bp
  40% of sequence in fragments shorter than     164 bp
  45% of sequence in fragments shorter than     185 bp
  50% of sequence in fragments shorter than     210 bp
  55% of sequence in fragments shorter than     237 bp
  60% of sequence in fragments shorter than     266 bp
  65% of sequence in fragments shorter than     299 bp
  70% of sequence in fragments shorter than     339 bp
  75% of sequence in fragments shorter than     380 bp
  80% of sequence in fragments shorter than     434 bp
  85% of sequence in fragments shorter than     506 bp
  90% of sequence in fragments shorter than     611 bp
  95% of sequence in fragments shorter than     778 bp

# BglII.MnlI (AGATCT+CCTC) double digest, 28513 thousand fragments (774732+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      94 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     144 bp
  40% of sequence in fragments shorter than     165 bp
  45% of sequence in fragments shorter than     186 bp
  50% of sequence in fragments shorter than     211 bp
  55% of sequence in fragments shorter than     239 bp
  60% of sequence in fragments shorter than     268 bp
  65% of sequence in fragments shorter than     302 bp
  70% of sequence in fragments shorter than     340 bp
  75% of sequence in fragments shorter than     382 bp
  80% of sequence in fragments shorter than     436 bp
  85% of sequence in fragments shorter than     508 bp
  90% of sequence in fragments shorter than     613 bp
  95% of sequence in fragments shorter than     780 bp

# MnlI.NcoI (CCTC+CCATGG) double digest, 28498 thousand fragments (27739501+759594 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     144 bp
  40% of sequence in fragments shorter than     165 bp
  45% of sequence in fragments shorter than     187 bp
  50% of sequence in fragments shorter than     212 bp
  55% of sequence in fragments shorter than     239 bp
  60% of sequence in fragments shorter than     268 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     343 bp
  75% of sequence in fragments shorter than     386 bp
  80% of sequence in fragments shorter than     444 bp
  85% of sequence in fragments shorter than     518 bp
  90% of sequence in fragments shorter than     627 bp
  95% of sequence in fragments shorter than     799 bp

# BclI.MnlI (TGATCA+CCTC) double digest, 28477 thousand fragments (738785+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     144 bp
  40% of sequence in fragments shorter than     165 bp
  45% of sequence in fragments shorter than     186 bp
  50% of sequence in fragments shorter than     211 bp
  55% of sequence in fragments shorter than     239 bp
  60% of sequence in fragments shorter than     267 bp
  65% of sequence in fragments shorter than     301 bp
  70% of sequence in fragments shorter than     340 bp
  75% of sequence in fragments shorter than     382 bp
  80% of sequence in fragments shorter than     436 bp
  85% of sequence in fragments shorter than     509 bp
  90% of sequence in fragments shorter than     613 bp
  95% of sequence in fragments shorter than     780 bp

# FnuDII.MnlI (CGCG+CCTC) double digest, 28472 thousand fragments (733940+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     217 bp
  55% of sequence in fragments shorter than     247 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     313 bp
  70% of sequence in fragments shorter than     352 bp
  75% of sequence in fragments shorter than     398 bp
  80% of sequence in fragments shorter than     455 bp
  85% of sequence in fragments shorter than     531 bp
  90% of sequence in fragments shorter than     642 bp
  95% of sequence in fragments shorter than     819 bp

# BsaAI.MnlI (YACGTR+CCTC) double digest, 28452 thousand fragments (713801+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     144 bp
  40% of sequence in fragments shorter than     165 bp
  45% of sequence in fragments shorter than     186 bp
  50% of sequence in fragments shorter than     212 bp
  55% of sequence in fragments shorter than     240 bp
  60% of sequence in fragments shorter than     269 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     341 bp
  75% of sequence in fragments shorter than     384 bp
  80% of sequence in fragments shorter than     440 bp
  85% of sequence in fragments shorter than     513 bp
  90% of sequence in fragments shorter than     619 bp
  95% of sequence in fragments shorter than     789 bp

# BceAI.MnlI (ACGGC+CCTC) double digest, 28444 thousand fragments (705178+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     145 bp
  40% of sequence in fragments shorter than     166 bp
  45% of sequence in fragments shorter than     188 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     243 bp
  60% of sequence in fragments shorter than     273 bp
  65% of sequence in fragments shorter than     308 bp
  70% of sequence in fragments shorter than     347 bp
  75% of sequence in fragments shorter than     392 bp
  80% of sequence in fragments shorter than     448 bp
  85% of sequence in fragments shorter than     523 bp
  90% of sequence in fragments shorter than     633 bp
  95% of sequence in fragments shorter than     807 bp

# BciVI.MnlI (GTATCC+CCTC) double digest, 28435 thousand fragments (696093+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     144 bp
  40% of sequence in fragments shorter than     165 bp
  45% of sequence in fragments shorter than     186 bp
  50% of sequence in fragments shorter than     211 bp
  55% of sequence in fragments shorter than     239 bp
  60% of sequence in fragments shorter than     268 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     342 bp
  75% of sequence in fragments shorter than     384 bp
  80% of sequence in fragments shorter than     438 bp
  85% of sequence in fragments shorter than     512 bp
  90% of sequence in fragments shorter than     618 bp
  95% of sequence in fragments shorter than     786 bp

# MboII.MseI (GAAGA+TTAA) double digest, 28413 thousand fragments (9199496+19214674 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      33 bp
  10% of sequence in fragments shorter than      51 bp
  15% of sequence in fragments shorter than      68 bp
  20% of sequence in fragments shorter than      83 bp
  25% of sequence in fragments shorter than      98 bp
  30% of sequence in fragments shorter than     114 bp
  35% of sequence in fragments shorter than     131 bp
  40% of sequence in fragments shorter than     149 bp
  45% of sequence in fragments shorter than     169 bp
  50% of sequence in fragments shorter than     189 bp
  55% of sequence in fragments shorter than     212 bp
  60% of sequence in fragments shorter than     240 bp
  65% of sequence in fragments shorter than     271 bp
  70% of sequence in fragments shorter than     307 bp
  75% of sequence in fragments shorter than     346 bp
  80% of sequence in fragments shorter than     394 bp
  85% of sequence in fragments shorter than     458 bp
  90% of sequence in fragments shorter than     551 bp
  95% of sequence in fragments shorter than     729 bp

# AflII.MnlI (CTTAAG+CCTC) double digest, 28382 thousand fragments (643284+27739501 sites), 104 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     144 bp
  40% of sequence in fragments shorter than     165 bp
  45% of sequence in fragments shorter than     186 bp
  50% of sequence in fragments shorter than     212 bp
  55% of sequence in fragments shorter than     239 bp
  60% of sequence in fragments shorter than     268 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     342 bp
  75% of sequence in fragments shorter than     384 bp
  80% of sequence in fragments shorter than     438 bp
  85% of sequence in fragments shorter than     511 bp
  90% of sequence in fragments shorter than     615 bp
  95% of sequence in fragments shorter than     782 bp

# HaeII.MnlI (RGCGCY+CCTC) double digest, 28369 thousand fragments (630101+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      54 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     189 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     244 bp
  60% of sequence in fragments shorter than     274 bp
  65% of sequence in fragments shorter than     310 bp
  70% of sequence in fragments shorter than     349 bp
  75% of sequence in fragments shorter than     395 bp
  80% of sequence in fragments shorter than     453 bp
  85% of sequence in fragments shorter than     528 bp
  90% of sequence in fragments shorter than     639 bp
  95% of sequence in fragments shorter than     817 bp

# MnlI.SacI (CCTC+GAGCTC) double digest, 28342 thousand fragments (27739501+603323 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     145 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     189 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     243 bp
  60% of sequence in fragments shorter than     273 bp
  65% of sequence in fragments shorter than     308 bp
  70% of sequence in fragments shorter than     346 bp
  75% of sequence in fragments shorter than     391 bp
  80% of sequence in fragments shorter than     447 bp
  85% of sequence in fragments shorter than     521 bp
  90% of sequence in fragments shorter than     631 bp
  95% of sequence in fragments shorter than     804 bp

# AvrII.MnlI (CCTAGG+CCTC) double digest, 28333 thousand fragments (594956+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     145 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     188 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     242 bp
  60% of sequence in fragments shorter than     272 bp
  65% of sequence in fragments shorter than     307 bp
  70% of sequence in fragments shorter than     346 bp
  75% of sequence in fragments shorter than     389 bp
  80% of sequence in fragments shorter than     445 bp
  85% of sequence in fragments shorter than     515 bp
  90% of sequence in fragments shorter than     620 bp
  95% of sequence in fragments shorter than     800 bp

# HgaI.MnlI (GACGC+CCTC) double digest, 28310 thousand fragments (571889+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      75 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     189 bp
  50% of sequence in fragments shorter than     216 bp
  55% of sequence in fragments shorter than     245 bp
  60% of sequence in fragments shorter than     274 bp
  65% of sequence in fragments shorter than     310 bp
  70% of sequence in fragments shorter than     349 bp
  75% of sequence in fragments shorter than     395 bp
  80% of sequence in fragments shorter than     452 bp
  85% of sequence in fragments shorter than     526 bp
  90% of sequence in fragments shorter than     637 bp
  95% of sequence in fragments shorter than     812 bp

# EciI.MnlI (GGCGGA+CCTC) double digest, 28310 thousand fragments (571273+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     192 bp
  50% of sequence in fragments shorter than     219 bp
  55% of sequence in fragments shorter than     248 bp
  60% of sequence in fragments shorter than     278 bp
  65% of sequence in fragments shorter than     314 bp
  70% of sequence in fragments shorter than     353 bp
  75% of sequence in fragments shorter than     400 bp
  80% of sequence in fragments shorter than     457 bp
  85% of sequence in fragments shorter than     533 bp
  90% of sequence in fragments shorter than     644 bp
  95% of sequence in fragments shorter than     821 bp

# MfeI.MnlI (CAATTG+CCTC) double digest, 28303 thousand fragments (564303+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     145 bp
  40% of sequence in fragments shorter than     166 bp
  45% of sequence in fragments shorter than     187 bp
  50% of sequence in fragments shorter than     212 bp
  55% of sequence in fragments shorter than     240 bp
  60% of sequence in fragments shorter than     269 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     342 bp
  75% of sequence in fragments shorter than     384 bp
  80% of sequence in fragments shorter than     438 bp
  85% of sequence in fragments shorter than     509 bp
  90% of sequence in fragments shorter than     614 bp
  95% of sequence in fragments shorter than     779 bp

# MnlI.SphI (CCTC+GCATGC) double digest, 28289 thousand fragments (27739501+550952 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     189 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     244 bp
  60% of sequence in fragments shorter than     273 bp
  65% of sequence in fragments shorter than     308 bp
  70% of sequence in fragments shorter than     347 bp
  75% of sequence in fragments shorter than     392 bp
  80% of sequence in fragments shorter than     448 bp
  85% of sequence in fragments shorter than     522 bp
  90% of sequence in fragments shorter than     631 bp
  95% of sequence in fragments shorter than     804 bp

# MnlI.ScaI (CCTC+AGTACT) double digest, 28282 thousand fragments (27739501+543793 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     145 bp
  40% of sequence in fragments shorter than     166 bp
  45% of sequence in fragments shorter than     187 bp
  50% of sequence in fragments shorter than     213 bp
  55% of sequence in fragments shorter than     241 bp
  60% of sequence in fragments shorter than     270 bp
  65% of sequence in fragments shorter than     304 bp
  70% of sequence in fragments shorter than     343 bp
  75% of sequence in fragments shorter than     385 bp
  80% of sequence in fragments shorter than     439 bp
  85% of sequence in fragments shorter than     512 bp
  90% of sequence in fragments shorter than     618 bp
  95% of sequence in fragments shorter than     785 bp

# ApaLI.MnlI (GTGCAC+CCTC) double digest, 28235 thousand fragments (496255+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     189 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     244 bp
  60% of sequence in fragments shorter than     273 bp
  65% of sequence in fragments shorter than     309 bp
  70% of sequence in fragments shorter than     347 bp
  75% of sequence in fragments shorter than     392 bp
  80% of sequence in fragments shorter than     449 bp
  85% of sequence in fragments shorter than     523 bp
  90% of sequence in fragments shorter than     632 bp
  95% of sequence in fragments shorter than     805 bp

# ApaI.MnlI (GGGCCC+CCTC) double digest, 28199 thousand fragments (460339+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     218 bp
  55% of sequence in fragments shorter than     247 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     313 bp
  70% of sequence in fragments shorter than     351 bp
  75% of sequence in fragments shorter than     398 bp
  80% of sequence in fragments shorter than     455 bp
  85% of sequence in fragments shorter than     530 bp
  90% of sequence in fragments shorter than     642 bp
  95% of sequence in fragments shorter than     819 bp

# EcoRV.MnlI (GATATC+CCTC) double digest, 28185 thousand fragments (446473+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     166 bp
  45% of sequence in fragments shorter than     188 bp
  50% of sequence in fragments shorter than     214 bp
  55% of sequence in fragments shorter than     242 bp
  60% of sequence in fragments shorter than     271 bp
  65% of sequence in fragments shorter than     305 bp
  70% of sequence in fragments shorter than     344 bp
  75% of sequence in fragments shorter than     387 bp
  80% of sequence in fragments shorter than     442 bp
  85% of sequence in fragments shorter than     515 bp
  90% of sequence in fragments shorter than     621 bp
  95% of sequence in fragments shorter than     791 bp

# Hpy188I.MseI (TCNGA+TTAA) double digest, 28156 thousand fragments (8942153+19214674 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      33 bp
  10% of sequence in fragments shorter than      51 bp
  15% of sequence in fragments shorter than      67 bp
  20% of sequence in fragments shorter than      83 bp
  25% of sequence in fragments shorter than      99 bp
  30% of sequence in fragments shorter than     115 bp
  35% of sequence in fragments shorter than     132 bp
  40% of sequence in fragments shorter than     150 bp
  45% of sequence in fragments shorter than     169 bp
  50% of sequence in fragments shorter than     187 bp
  55% of sequence in fragments shorter than     210 bp
  60% of sequence in fragments shorter than     236 bp
  65% of sequence in fragments shorter than     266 bp
  70% of sequence in fragments shorter than     300 bp
  75% of sequence in fragments shorter than     338 bp
  80% of sequence in fragments shorter than     382 bp
  85% of sequence in fragments shorter than     442 bp
  90% of sequence in fragments shorter than     530 bp
  95% of sequence in fragments shorter than     687 bp

# MnlI.SpeI (CCTC+ACTAGT) double digest, 28139 thousand fragments (27739501+400286 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     189 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     243 bp
  60% of sequence in fragments shorter than     272 bp
  65% of sequence in fragments shorter than     307 bp
  70% of sequence in fragments shorter than     346 bp
  75% of sequence in fragments shorter than     389 bp
  80% of sequence in fragments shorter than     444 bp
  85% of sequence in fragments shorter than     517 bp
  90% of sequence in fragments shorter than     624 bp
  95% of sequence in fragments shorter than     794 bp

# HinfI.MseI (GANTC+TTAA) double digest, 28130 thousand fragments (8916054+19214674 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      34 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      69 bp
  20% of sequence in fragments shorter than      83 bp
  25% of sequence in fragments shorter than      99 bp
  30% of sequence in fragments shorter than     115 bp
  35% of sequence in fragments shorter than     131 bp
  40% of sequence in fragments shorter than     148 bp
  45% of sequence in fragments shorter than     166 bp
  50% of sequence in fragments shorter than     185 bp
  55% of sequence in fragments shorter than     206 bp
  60% of sequence in fragments shorter than     227 bp
  65% of sequence in fragments shorter than     253 bp
  70% of sequence in fragments shorter than     282 bp
  75% of sequence in fragments shorter than     310 bp
  80% of sequence in fragments shorter than     347 bp
  85% of sequence in fragments shorter than     399 bp
  90% of sequence in fragments shorter than     476 bp
  95% of sequence in fragments shorter than     617 bp

# HpaI.MnlI (GTTAAC+CCTC) double digest, 28128 thousand fragments (389418+27739501 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     189 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     243 bp
  60% of sequence in fragments shorter than     272 bp
  65% of sequence in fragments shorter than     307 bp
  70% of sequence in fragments shorter than     346 bp
  75% of sequence in fragments shorter than     389 bp
  80% of sequence in fragments shorter than     444 bp
  85% of sequence in fragments shorter than     517 bp
  90% of sequence in fragments shorter than     625 bp
  95% of sequence in fragments shorter than     796 bp

# CviAII.DdeI (CATG+CTNAG) double digest, 28127 thousand fragments (13815697+14312045 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      35 bp
  10% of sequence in fragments shorter than      52 bp
  15% of sequence in fragments shorter than      68 bp
  20% of sequence in fragments shorter than      84 bp
  25% of sequence in fragments shorter than      99 bp
  30% of sequence in fragments shorter than     114 bp
  35% of sequence in fragments shorter than     130 bp
  40% of sequence in fragments shorter than     145 bp
  45% of sequence in fragments shorter than     162 bp
  50% of sequence in fragments shorter than     176 bp
  55% of sequence in fragments shorter than     195 bp
  60% of sequence in fragments shorter than     216 bp
  65% of sequence in fragments shorter than     239 bp
  70% of sequence in fragments shorter than     264 bp
  75% of sequence in fragments shorter than     293 bp
  80% of sequence in fragments shorter than     329 bp
  85% of sequence in fragments shorter than     374 bp
  90% of sequence in fragments shorter than     440 bp
  95% of sequence in fragments shorter than     552 bp

# MnlI.SapI (CCTC+GCTCTTC) double digest, 28116 thousand fragments (27739501+377161 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     190 bp
  50% of sequence in fragments shorter than     216 bp
  55% of sequence in fragments shorter than     245 bp
  60% of sequence in fragments shorter than     274 bp
  65% of sequence in fragments shorter than     310 bp
  70% of sequence in fragments shorter than     348 bp
  75% of sequence in fragments shorter than     393 bp
  80% of sequence in fragments shorter than     449 bp
  85% of sequence in fragments shorter than     524 bp
  90% of sequence in fragments shorter than     634 bp
  95% of sequence in fragments shorter than     807 bp

# MnlI.SmaI (CCTC+CCCGGG) double digest, 28115 thousand fragments (27739501+376940 sites), 105 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     192 bp
  50% of sequence in fragments shorter than     219 bp
  55% of sequence in fragments shorter than     248 bp
  60% of sequence in fragments shorter than     278 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     353 bp
  75% of sequence in fragments shorter than     400 bp
  80% of sequence in fragments shorter than     457 bp
  85% of sequence in fragments shorter than     533 bp
  90% of sequence in fragments shorter than     644 bp
  95% of sequence in fragments shorter than     822 bp

# BamHI.MnlI (GGATCC+CCTC) double digest, 28104 thousand fragments (365999+27739501 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     190 bp
  50% of sequence in fragments shorter than     217 bp
  55% of sequence in fragments shorter than     246 bp
  60% of sequence in fragments shorter than     275 bp
  65% of sequence in fragments shorter than     311 bp
  70% of sequence in fragments shorter than     349 bp
  75% of sequence in fragments shorter than     395 bp
  80% of sequence in fragments shorter than     452 bp
  85% of sequence in fragments shorter than     526 bp
  90% of sequence in fragments shorter than     632 bp
  95% of sequence in fragments shorter than     813 bp

# BstEII.MnlI (GGTNACC+CCTC) double digest, 28074 thousand fragments (335865+27739501 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     217 bp
  55% of sequence in fragments shorter than     246 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     311 bp
  70% of sequence in fragments shorter than     350 bp
  75% of sequence in fragments shorter than     396 bp
  80% of sequence in fragments shorter than     453 bp
  85% of sequence in fragments shorter than     527 bp
  90% of sequence in fragments shorter than     637 bp
  95% of sequence in fragments shorter than     812 bp

# MnlI.SexAI (CCTC+ACCWGGT) double digest, 28036 thousand fragments (27739501+297900 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     217 bp
  55% of sequence in fragments shorter than     246 bp
  60% of sequence in fragments shorter than     275 bp
  65% of sequence in fragments shorter than     311 bp
  70% of sequence in fragments shorter than     350 bp
  75% of sequence in fragments shorter than     396 bp
  80% of sequence in fragments shorter than     453 bp
  85% of sequence in fragments shorter than     527 bp
  90% of sequence in fragments shorter than     638 bp
  95% of sequence in fragments shorter than     813 bp

# KpnI.MnlI (GGTACC+CCTC) double digest, 28026 thousand fragments (287974+27739501 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      76 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     169 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     218 bp
  55% of sequence in fragments shorter than     247 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     312 bp
  70% of sequence in fragments shorter than     350 bp
  75% of sequence in fragments shorter than     396 bp
  80% of sequence in fragments shorter than     453 bp
  85% of sequence in fragments shorter than     527 bp
  90% of sequence in fragments shorter than     638 bp
  95% of sequence in fragments shorter than     813 bp

# MnlI.NheI (CCTC+GCTAGC) double digest, 28026 thousand fragments (27739501+287384 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     190 bp
  50% of sequence in fragments shorter than     217 bp
  55% of sequence in fragments shorter than     245 bp
  60% of sequence in fragments shorter than     274 bp
  65% of sequence in fragments shorter than     310 bp
  70% of sequence in fragments shorter than     348 bp
  75% of sequence in fragments shorter than     394 bp
  80% of sequence in fragments shorter than     450 bp
  85% of sequence in fragments shorter than     523 bp
  90% of sequence in fragments shorter than     627 bp
  95% of sequence in fragments shorter than     808 bp

# MseI.TspRI (TTAA+CASTG) double digest, 27979 thousand fragments (19214674+8765240 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      34 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      70 bp
  20% of sequence in fragments shorter than      85 bp
  25% of sequence in fragments shorter than     101 bp
  30% of sequence in fragments shorter than     116 bp
  35% of sequence in fragments shorter than     132 bp
  40% of sequence in fragments shorter than     148 bp
  45% of sequence in fragments shorter than     166 bp
  50% of sequence in fragments shorter than     185 bp
  55% of sequence in fragments shorter than     206 bp
  60% of sequence in fragments shorter than     226 bp
  65% of sequence in fragments shorter than     247 bp
  70% of sequence in fragments shorter than     272 bp
  75% of sequence in fragments shorter than     304 bp
  80% of sequence in fragments shorter than     344 bp
  85% of sequence in fragments shorter than     394 bp
  90% of sequence in fragments shorter than     469 bp
  95% of sequence in fragments shorter than     623 bp

# BsrBI.MnlI (CCGCTC+CCTC) double digest, 27974 thousand fragments (235523+27739501 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     169 bp
  45% of sequence in fragments shorter than     192 bp
  50% of sequence in fragments shorter than     219 bp
  55% of sequence in fragments shorter than     248 bp
  60% of sequence in fragments shorter than     278 bp
  65% of sequence in fragments shorter than     314 bp
  70% of sequence in fragments shorter than     353 bp
  75% of sequence in fragments shorter than     399 bp
  80% of sequence in fragments shorter than     457 bp
  85% of sequence in fragments shorter than     532 bp
  90% of sequence in fragments shorter than     643 bp
  95% of sequence in fragments shorter than     821 bp

# KasI.MnlI (GGCGCC+CCTC) double digest, 27969 thousand fragments (230381+27739501 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     823 bp

# MnlI.SwaI (CCTC+ATTTAAAT) double digest, 27965 thousand fragments (27739501+226602 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     168 bp
  45% of sequence in fragments shorter than     190 bp
  50% of sequence in fragments shorter than     217 bp
  55% of sequence in fragments shorter than     245 bp
  60% of sequence in fragments shorter than     274 bp
  65% of sequence in fragments shorter than     309 bp
  70% of sequence in fragments shorter than     347 bp
  75% of sequence in fragments shorter than     392 bp
  80% of sequence in fragments shorter than     447 bp
  85% of sequence in fragments shorter than     520 bp
  90% of sequence in fragments shorter than     627 bp
  95% of sequence in fragments shorter than     797 bp

# AclI.MnlI (AACGTT+CCTC) double digest, 27911 thousand fragments (172706+27739501 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     169 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     218 bp
  55% of sequence in fragments shorter than     246 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     311 bp
  70% of sequence in fragments shorter than     350 bp
  75% of sequence in fragments shorter than     396 bp
  80% of sequence in fragments shorter than     452 bp
  85% of sequence in fragments shorter than     525 bp
  90% of sequence in fragments shorter than     635 bp
  95% of sequence in fragments shorter than     811 bp

# MnlI.PacI (CCTC+TTAATTAA) double digest, 27898 thousand fragments (27739501+159455 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     169 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     218 bp
  55% of sequence in fragments shorter than     247 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     312 bp
  70% of sequence in fragments shorter than     350 bp
  75% of sequence in fragments shorter than     395 bp
  80% of sequence in fragments shorter than     452 bp
  85% of sequence in fragments shorter than     525 bp
  90% of sequence in fragments shorter than     635 bp
  95% of sequence in fragments shorter than     808 bp

# Hpy99I.MnlI (CGWCG+CCTC) double digest, 27896 thousand fragments (157917+27739501 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     192 bp
  50% of sequence in fragments shorter than     219 bp
  55% of sequence in fragments shorter than     248 bp
  60% of sequence in fragments shorter than     278 bp
  65% of sequence in fragments shorter than     314 bp
  70% of sequence in fragments shorter than     353 bp
  75% of sequence in fragments shorter than     400 bp
  80% of sequence in fragments shorter than     457 bp
  85% of sequence in fragments shorter than     532 bp
  90% of sequence in fragments shorter than     644 bp
  95% of sequence in fragments shorter than     821 bp

# MnlI.SnaBI (CCTC+TACGTA) double digest, 27871 thousand fragments (27739501+132299 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     169 bp
  45% of sequence in fragments shorter than     192 bp
  50% of sequence in fragments shorter than     218 bp
  55% of sequence in fragments shorter than     247 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     312 bp
  70% of sequence in fragments shorter than     351 bp
  75% of sequence in fragments shorter than     397 bp
  80% of sequence in fragments shorter than     453 bp
  85% of sequence in fragments shorter than     527 bp
  90% of sequence in fragments shorter than     638 bp
  95% of sequence in fragments shorter than     812 bp

# MnlI.NaeI (CCTC+GCCGGC) double digest, 27864 thousand fragments (27739501+125692 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     533 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     823 bp

# MnlI.XhoI (CCTC+CTCGAG) double digest, 27860 thousand fragments (27739501+121308 sites), 106 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     400 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     533 bp
  90% of sequence in fragments shorter than     644 bp
  95% of sequence in fragments shorter than     822 bp

# ClaI.MnlI (ATCGAT+CCTC) double digest, 27825 thousand fragments (86477+27739501 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     192 bp
  50% of sequence in fragments shorter than     219 bp
  55% of sequence in fragments shorter than     248 bp
  60% of sequence in fragments shorter than     277 bp
  65% of sequence in fragments shorter than     314 bp
  70% of sequence in fragments shorter than     352 bp
  75% of sequence in fragments shorter than     399 bp
  80% of sequence in fragments shorter than     455 bp
  85% of sequence in fragments shorter than     530 bp
  90% of sequence in fragments shorter than     642 bp
  95% of sequence in fragments shorter than     817 bp

# AatII.MnlI (GACGTC+CCTC) double digest, 27810 thousand fragments (71089+27739501 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      97 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     148 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     278 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     353 bp
  75% of sequence in fragments shorter than     400 bp
  80% of sequence in fragments shorter than     457 bp
  85% of sequence in fragments shorter than     533 bp
  90% of sequence in fragments shorter than     644 bp
  95% of sequence in fragments shorter than     821 bp

# MnlI.SacII (CCTC+CCGCGG) double digest, 27805 thousand fragments (27739501+66901 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     823 bp

# MnlI.PspXI (CCTC+VCTCGAGB) double digest, 27798 thousand fragments (27739501+59193 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     823 bp

# AgeI.MnlI (ACCGGT+CCTC) double digest, 27792 thousand fragments (53386+27739501 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     278 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     353 bp
  75% of sequence in fragments shorter than     400 bp
  80% of sequence in fragments shorter than     457 bp
  85% of sequence in fragments shorter than     532 bp
  90% of sequence in fragments shorter than     644 bp
  95% of sequence in fragments shorter than     821 bp

# MnlI.PmeI (CCTC+GTTTAAAC) double digest, 27781 thousand fragments (27739501+42094 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     278 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     353 bp
  75% of sequence in fragments shorter than     400 bp
  80% of sequence in fragments shorter than     457 bp
  85% of sequence in fragments shorter than     532 bp
  90% of sequence in fragments shorter than     643 bp
  95% of sequence in fragments shorter than     820 bp

# HaeIII.MseI (GGCC+TTAA) double digest, 27776 thousand fragments (8562230+19214674 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      36 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      69 bp
  20% of sequence in fragments shorter than      85 bp
  25% of sequence in fragments shorter than     100 bp
  30% of sequence in fragments shorter than     116 bp
  35% of sequence in fragments shorter than     132 bp
  40% of sequence in fragments shorter than     149 bp
  45% of sequence in fragments shorter than     168 bp
  50% of sequence in fragments shorter than     187 bp
  55% of sequence in fragments shorter than     208 bp
  60% of sequence in fragments shorter than     230 bp
  65% of sequence in fragments shorter than     255 bp
  70% of sequence in fragments shorter than     280 bp
  75% of sequence in fragments shorter than     309 bp
  80% of sequence in fragments shorter than     345 bp
  85% of sequence in fragments shorter than     390 bp
  90% of sequence in fragments shorter than     457 bp
  95% of sequence in fragments shorter than     568 bp

# MnlI.SalI (CCTC+GTCGAC) double digest, 27770 thousand fragments (27739501+31396 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     533 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     822 bp

# MluI.MnlI (ACGCGT+CCTC) double digest, 27760 thousand fragments (21415+27739501 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     823 bp

# MnlI.SgrAI (CCTC+CRCCGGYG) double digest, 27754 thousand fragments (27739501+15270 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     316 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     823 bp

# MnlI.NruI (CCTC+TCGCGA) double digest, 27753 thousand fragments (27739501+14872 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     316 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     823 bp

# MnlI.PvuI (CCTC+CGATCG) double digest, 27753 thousand fragments (27739501+14186 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     315 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     823 bp

# FseI.MnlI (GGCCGGCC+CCTC) double digest, 27752 thousand fragments (13662+27739501 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     316 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     646 bp
  95% of sequence in fragments shorter than     823 bp

# MnlI.NotI (CCTC+GCGGCCGC) double digest, 27748 thousand fragments (27739501+9795 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     316 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     646 bp
  95% of sequence in fragments shorter than     824 bp

# MnlI.RsrII (CCTC+CGGWCCG) double digest, 27748 thousand fragments (27739501+9764 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     316 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     646 bp
  95% of sequence in fragments shorter than     823 bp

# AscI.MnlI (GGCGCGCC+CCTC) double digest, 27743 thousand fragments (4751+27739501 sites), 107 bp average fragment length
   5% of sequence in fragments shorter than      32 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     131 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     193 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     279 bp
  65% of sequence in fragments shorter than     316 bp
  70% of sequence in fragments shorter than     354 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     458 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     646 bp
  95% of sequence in fragments shorter than     824 bp

# AluI.DdeI (AGCT+CTNAG) double digest, 27339 thousand fragments (13027778+14312045 sites), 109 bp average fragment length
   5% of sequence in fragments shorter than      37 bp
  10% of sequence in fragments shorter than      59 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     128 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     165 bp
  45% of sequence in fragments shorter than     181 bp
  50% of sequence in fragments shorter than     202 bp
  55% of sequence in fragments shorter than     223 bp
  60% of sequence in fragments shorter than     247 bp
  65% of sequence in fragments shorter than     273 bp
  70% of sequence in fragments shorter than     302 bp
  75% of sequence in fragments shorter than     335 bp
  80% of sequence in fragments shorter than     375 bp
  85% of sequence in fragments shorter than     429 bp
  90% of sequence in fragments shorter than     501 bp
  95% of sequence in fragments shorter than     630 bp

# AluI.CviAII (AGCT+CATG) double digest, 26842 thousand fragments (13027778+13815697 sites), 111 bp average fragment length
   5% of sequence in fragments shorter than      37 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      88 bp
  25% of sequence in fragments shorter than     104 bp
  30% of sequence in fragments shorter than     120 bp
  35% of sequence in fragments shorter than     136 bp
  40% of sequence in fragments shorter than     153 bp
  45% of sequence in fragments shorter than     170 bp
  50% of sequence in fragments shorter than     188 bp
  55% of sequence in fragments shorter than     208 bp
  60% of sequence in fragments shorter than     229 bp
  65% of sequence in fragments shorter than     253 bp
  70% of sequence in fragments shorter than     279 bp
  75% of sequence in fragments shorter than     310 bp
  80% of sequence in fragments shorter than     346 bp
  85% of sequence in fragments shorter than     394 bp
  90% of sequence in fragments shorter than     463 bp
  95% of sequence in fragments shorter than     583 bp

# BspNCI.MseI (CCAGA+TTAA) double digest, 26496 thousand fragments (7282436+19214674 sites), 112 bp average fragment length
   5% of sequence in fragments shorter than      36 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      73 bp
  20% of sequence in fragments shorter than      89 bp
  25% of sequence in fragments shorter than     106 bp
  30% of sequence in fragments shorter than     123 bp
  35% of sequence in fragments shorter than     140 bp
  40% of sequence in fragments shorter than     158 bp
  45% of sequence in fragments shorter than     177 bp
  50% of sequence in fragments shorter than     198 bp
  55% of sequence in fragments shorter than     221 bp
  60% of sequence in fragments shorter than     248 bp
  65% of sequence in fragments shorter than     277 bp
  70% of sequence in fragments shorter than     309 bp
  75% of sequence in fragments shorter than     345 bp
  80% of sequence in fragments shorter than     388 bp
  85% of sequence in fragments shorter than     446 bp
  90% of sequence in fragments shorter than     528 bp
  95% of sequence in fragments shorter than     675 bp

# BspKT6I.MseI (GATC+TTAA) double digest, 26413 thousand fragments (7199434+19214674 sites), 112 bp average fragment length
   5% of sequence in fragments shorter than      37 bp
  10% of sequence in fragments shorter than      57 bp
  15% of sequence in fragments shorter than      74 bp
  20% of sequence in fragments shorter than      90 bp
  25% of sequence in fragments shorter than     106 bp
  30% of sequence in fragments shorter than     124 bp
  35% of sequence in fragments shorter than     140 bp
  40% of sequence in fragments shorter than     158 bp
  45% of sequence in fragments shorter than     176 bp
  50% of sequence in fragments shorter than     193 bp
  55% of sequence in fragments shorter than     216 bp
  60% of sequence in fragments shorter than     242 bp
  65% of sequence in fragments shorter than     270 bp
  70% of sequence in fragments shorter than     302 bp
  75% of sequence in fragments shorter than     339 bp
  80% of sequence in fragments shorter than     384 bp
  85% of sequence in fragments shorter than     450 bp
  90% of sequence in fragments shorter than     545 bp
  95% of sequence in fragments shorter than     749 bp

# Hin4II.MseI (CCTTC+TTAA) double digest, 26274 thousand fragments (7059915+19214674 sites), 113 bp average fragment length
   5% of sequence in fragments shorter than      36 bp
  10% of sequence in fragments shorter than      56 bp
  15% of sequence in fragments shorter than      74 bp
  20% of sequence in fragments shorter than      90 bp
  25% of sequence in fragments shorter than     107 bp
  30% of sequence in fragments shorter than     125 bp
  35% of sequence in fragments shorter than     143 bp
  40% of sequence in fragments shorter than     162 bp
  45% of sequence in fragments shorter than     181 bp
  50% of sequence in fragments shorter than     203 bp
  55% of sequence in fragments shorter than     227 bp
  60% of sequence in fragments shorter than     255 bp
  65% of sequence in fragments shorter than     285 bp
  70% of sequence in fragments shorter than     319 bp
  75% of sequence in fragments shorter than     357 bp
  80% of sequence in fragments shorter than     405 bp
  85% of sequence in fragments shorter than     466 bp
  90% of sequence in fragments shorter than     556 bp
  95% of sequence in fragments shorter than     720 bp

# DdeI.ScrFI (CTNAG+CCNGG) double digest, 26116 thousand fragments (14312045+11805099 sites), 114 bp average fragment length
   5% of sequence in fragments shorter than      35 bp
  10% of sequence in fragments shorter than      51 bp
  15% of sequence in fragments shorter than      71 bp
  20% of sequence in fragments shorter than      88 bp
  25% of sequence in fragments shorter than     106 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     146 bp
  40% of sequence in fragments shorter than     169 bp
  45% of sequence in fragments shorter than     188 bp
  50% of sequence in fragments shorter than     214 bp
  55% of sequence in fragments shorter than     243 bp
  60% of sequence in fragments shorter than     275 bp
  65% of sequence in fragments shorter than     309 bp
  70% of sequence in fragments shorter than     347 bp
  75% of sequence in fragments shorter than     394 bp
  80% of sequence in fragments shorter than     451 bp
  85% of sequence in fragments shorter than     527 bp
  90% of sequence in fragments shorter than     632 bp
  95% of sequence in fragments shorter than     812 bp

# BsmAI.MseI (GTCTC+TTAA) double digest, 25845 thousand fragments (6631585+19214674 sites), 115 bp average fragment length
   5% of sequence in fragments shorter than      37 bp
  10% of sequence in fragments shorter than      58 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      94 bp
  25% of sequence in fragments shorter than     110 bp
  30% of sequence in fragments shorter than     127 bp
  35% of sequence in fragments shorter than     144 bp
  40% of sequence in fragments shorter than     161 bp
  45% of sequence in fragments shorter than     178 bp
  50% of sequence in fragments shorter than     198 bp
  55% of sequence in fragments shorter than     220 bp
  60% of sequence in fragments shorter than     247 bp
  65% of sequence in fragments shorter than     275 bp
  70% of sequence in fragments shorter than     305 bp
  75% of sequence in fragments shorter than     343 bp
  80% of sequence in fragments shorter than     388 bp
  85% of sequence in fragments shorter than     453 bp
  90% of sequence in fragments shorter than     551 bp
  95% of sequence in fragments shorter than     729 bp

# CviAII.ScrFI (CATG+CCNGG) double digest, 25620 thousand fragments (13815697+11805099 sites), 116 bp average fragment length
   5% of sequence in fragments shorter than      40 bp
  10% of sequence in fragments shorter than      55 bp
  15% of sequence in fragments shorter than      72 bp
  20% of sequence in fragments shorter than      89 bp
  25% of sequence in fragments shorter than     109 bp
  30% of sequence in fragments shorter than     126 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     167 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     242 bp
  60% of sequence in fragments shorter than     270 bp
  65% of sequence in fragments shorter than     302 bp
  70% of sequence in fragments shorter than     338 bp
  75% of sequence in fragments shorter than     382 bp
  80% of sequence in fragments shorter than     436 bp
  85% of sequence in fragments shorter than     509 bp
  90% of sequence in fragments shorter than     612 bp
  95% of sequence in fragments shorter than     810 bp

# ApoI.MseI (RAATTY+TTAA) double digest, 25596 thousand fragments (6382433+19214674 sites), 116 bp average fragment length
   5% of sequence in fragments shorter than      36 bp
  10% of sequence in fragments shorter than      57 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     116 bp
  30% of sequence in fragments shorter than     136 bp
  35% of sequence in fragments shorter than     157 bp
  40% of sequence in fragments shorter than     181 bp
  45% of sequence in fragments shorter than     208 bp
  50% of sequence in fragments shorter than     237 bp
  55% of sequence in fragments shorter than     270 bp
  60% of sequence in fragments shorter than     306 bp
  65% of sequence in fragments shorter than     344 bp
  70% of sequence in fragments shorter than     388 bp
  75% of sequence in fragments shorter than     445 bp
  80% of sequence in fragments shorter than     519 bp
  85% of sequence in fragments shorter than     624 bp
  90% of sequence in fragments shorter than     787 bp
  95% of sequence in fragments shorter than    1169 bp

# BthCI.MseI (GCNGC+TTAA) double digest, 25424 thousand fragments (6209929+19214674 sites), 117 bp average fragment length
   5% of sequence in fragments shorter than      38 bp
  10% of sequence in fragments shorter than      59 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     131 bp
  35% of sequence in fragments shorter than     149 bp
  40% of sequence in fragments shorter than     169 bp
  45% of sequence in fragments shorter than     190 bp
  50% of sequence in fragments shorter than     213 bp
  55% of sequence in fragments shorter than     237 bp
  60% of sequence in fragments shorter than     263 bp
  65% of sequence in fragments shorter than     294 bp
  70% of sequence in fragments shorter than     328 bp
  75% of sequence in fragments shorter than     364 bp
  80% of sequence in fragments shorter than     411 bp
  85% of sequence in fragments shorter than     470 bp
  90% of sequence in fragments shorter than     554 bp
  95% of sequence in fragments shorter than     707 bp

# BccI.MseI (CCATC+TTAA) double digest, 25385 thousand fragments (6170927+19214674 sites), 117 bp average fragment length
   5% of sequence in fragments shorter than      38 bp
  10% of sequence in fragments shorter than      59 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     130 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     166 bp
  45% of sequence in fragments shorter than     186 bp
  50% of sequence in fragments shorter than     207 bp
  55% of sequence in fragments shorter than     231 bp
  60% of sequence in fragments shorter than     257 bp
  65% of sequence in fragments shorter than     285 bp
  70% of sequence in fragments shorter than     316 bp
  75% of sequence in fragments shorter than     351 bp
  80% of sequence in fragments shorter than     397 bp
  85% of sequence in fragments shorter than     458 bp
  90% of sequence in fragments shorter than     551 bp
  95% of sequence in fragments shorter than     725 bp

# HphI.MseI (GGTGA+TTAA) double digest, 25221 thousand fragments (6007336+19214674 sites), 118 bp average fragment length
   5% of sequence in fragments shorter than      39 bp
  10% of sequence in fragments shorter than      59 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     113 bp
  30% of sequence in fragments shorter than     129 bp
  35% of sequence in fragments shorter than     147 bp
  40% of sequence in fragments shorter than     164 bp
  45% of sequence in fragments shorter than     184 bp
  50% of sequence in fragments shorter than     206 bp
  55% of sequence in fragments shorter than     229 bp
  60% of sequence in fragments shorter than     254 bp
  65% of sequence in fragments shorter than     282 bp
  70% of sequence in fragments shorter than     312 bp
  75% of sequence in fragments shorter than     346 bp
  80% of sequence in fragments shorter than     390 bp
  85% of sequence in fragments shorter than     449 bp
  90% of sequence in fragments shorter than     537 bp
  95% of sequence in fragments shorter than     693 bp

# BsrI.MseI (ACTGG+TTAA) double digest, 24955 thousand fragments (5741310+19214674 sites), 119 bp average fragment length
   5% of sequence in fragments shorter than      38 bp
  10% of sequence in fragments shorter than      59 bp
  15% of sequence in fragments shorter than      78 bp
  20% of sequence in fragments shorter than      96 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     132 bp
  35% of sequence in fragments shorter than     150 bp
  40% of sequence in fragments shorter than     170 bp
  45% of sequence in fragments shorter than     191 bp
  50% of sequence in fragments shorter than     215 bp
  55% of sequence in fragments shorter than     241 bp
  60% of sequence in fragments shorter than     270 bp
  65% of sequence in fragments shorter than     303 bp
  70% of sequence in fragments shorter than     339 bp
  75% of sequence in fragments shorter than     378 bp
  80% of sequence in fragments shorter than     429 bp
  85% of sequence in fragments shorter than     496 bp
  90% of sequence in fragments shorter than     598 bp
  95% of sequence in fragments shorter than     795 bp

# AluI.ScrFI (AGCT+CCNGG) double digest, 24832 thousand fragments (13027778+11805099 sites), 120 bp average fragment length
   5% of sequence in fragments shorter than      37 bp
  10% of sequence in fragments shorter than      53 bp
  15% of sequence in fragments shorter than      74 bp
  20% of sequence in fragments shorter than      90 bp
  25% of sequence in fragments shorter than     112 bp
  30% of sequence in fragments shorter than     133 bp
  35% of sequence in fragments shorter than     155 bp
  40% of sequence in fragments shorter than     178 bp
  45% of sequence in fragments shorter than     204 bp
  50% of sequence in fragments shorter than     230 bp
  55% of sequence in fragments shorter than     259 bp
  60% of sequence in fragments shorter than     293 bp
  65% of sequence in fragments shorter than     329 bp
  70% of sequence in fragments shorter than     369 bp
  75% of sequence in fragments shorter than     419 bp
  80% of sequence in fragments shorter than     480 bp
  85% of sequence in fragments shorter than     560 bp
  90% of sequence in fragments shorter than     679 bp
  95% of sequence in fragments shorter than     878 bp

# BbvI.MseI (GCAGC+TTAA) double digest, 24504 thousand fragments (5290051+19214674 sites), 121 bp average fragment length
   5% of sequence in fragments shorter than      40 bp
  10% of sequence in fragments shorter than      61 bp
  15% of sequence in fragments shorter than      80 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     116 bp
  30% of sequence in fragments shorter than     135 bp
  35% of sequence in fragments shorter than     154 bp
  40% of sequence in fragments shorter than     175 bp
  45% of sequence in fragments shorter than     197 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     245 bp
  60% of sequence in fragments shorter than     272 bp
  65% of sequence in fragments shorter than     305 bp
  70% of sequence in fragments shorter than     340 bp
  75% of sequence in fragments shorter than     377 bp
  80% of sequence in fragments shorter than     425 bp
  85% of sequence in fragments shorter than     486 bp
  90% of sequence in fragments shorter than     576 bp
  95% of sequence in fragments shorter than     736 bp

# FokI.MseI (GGATG+TTAA) double digest, 24415 thousand fragments (5201116+19214674 sites), 122 bp average fragment length
   5% of sequence in fragments shorter than      39 bp
  10% of sequence in fragments shorter than      61 bp
  15% of sequence in fragments shorter than      80 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     117 bp
  30% of sequence in fragments shorter than     135 bp
  35% of sequence in fragments shorter than     154 bp
  40% of sequence in fragments shorter than     174 bp
  45% of sequence in fragments shorter than     196 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     247 bp
  60% of sequence in fragments shorter than     275 bp
  65% of sequence in fragments shorter than     307 bp
  70% of sequence in fragments shorter than     341 bp
  75% of sequence in fragments shorter than     379 bp
  80% of sequence in fragments shorter than     430 bp
  85% of sequence in fragments shorter than     496 bp
  90% of sequence in fragments shorter than     596 bp
  95% of sequence in fragments shorter than     773 bp

# CviQI.MseI (GTAC+TTAA) double digest, 24329 thousand fragments (5115078+19214674 sites), 122 bp average fragment length
   5% of sequence in fragments shorter than      38 bp
  10% of sequence in fragments shorter than      59 bp
  15% of sequence in fragments shorter than      79 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     118 bp
  30% of sequence in fragments shorter than     137 bp
  35% of sequence in fragments shorter than     157 bp
  40% of sequence in fragments shorter than     180 bp
  45% of sequence in fragments shorter than     204 bp
  50% of sequence in fragments shorter than     232 bp
  55% of sequence in fragments shorter than     262 bp
  60% of sequence in fragments shorter than     297 bp
  65% of sequence in fragments shorter than     332 bp
  70% of sequence in fragments shorter than     370 bp
  75% of sequence in fragments shorter than     419 bp
  80% of sequence in fragments shorter than     482 bp
  85% of sequence in fragments shorter than     569 bp
  90% of sequence in fragments shorter than     698 bp
  95% of sequence in fragments shorter than     958 bp

# MseI.TfiI (TTAA+GAWTC) double digest, 24198 thousand fragments (19214674+4984362 sites), 123 bp average fragment length
   5% of sequence in fragments shorter than      39 bp
  10% of sequence in fragments shorter than      61 bp
  15% of sequence in fragments shorter than      80 bp
  20% of sequence in fragments shorter than      99 bp
  25% of sequence in fragments shorter than     118 bp
  30% of sequence in fragments shorter than     137 bp
  35% of sequence in fragments shorter than     156 bp
  40% of sequence in fragments shorter than     177 bp
  45% of sequence in fragments shorter than     200 bp
  50% of sequence in fragments shorter than     222 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     280 bp
  65% of sequence in fragments shorter than     311 bp
  70% of sequence in fragments shorter than     347 bp
  75% of sequence in fragments shorter than     392 bp
  80% of sequence in fragments shorter than     451 bp
  85% of sequence in fragments shorter than     528 bp
  90% of sequence in fragments shorter than     646 bp
  95% of sequence in fragments shorter than     881 bp

# BstNI.DdeI (CCWGG+CTNAG) double digest, 24167 thousand fragments (9855646+14312045 sites), 123 bp average fragment length
   5% of sequence in fragments shorter than      39 bp
  10% of sequence in fragments shorter than      57 bp
  15% of sequence in fragments shorter than      77 bp
  20% of sequence in fragments shorter than      95 bp
  25% of sequence in fragments shorter than     114 bp
  30% of sequence in fragments shorter than     134 bp
  35% of sequence in fragments shorter than     155 bp
  40% of sequence in fragments shorter than     174 bp
  45% of sequence in fragments shorter than     197 bp
  50% of sequence in fragments shorter than     224 bp
  55% of sequence in fragments shorter than     253 bp
  60% of sequence in fragments shorter than     285 bp
  65% of sequence in fragments shorter than     319 bp
  70% of sequence in fragments shorter than     357 bp
  75% of sequence in fragments shorter than     403 bp
  80% of sequence in fragments shorter than     462 bp
  85% of sequence in fragments shorter than     538 bp
  90% of sequence in fragments shorter than     644 bp
  95% of sequence in fragments shorter than     825 bp

# BstNI.CviAII (CCWGG+CATG) double digest, 23670 thousand fragments (9855646+13815697 sites), 125 bp average fragment length
   5% of sequence in fragments shorter than      42 bp
  10% of sequence in fragments shorter than      61 bp
  15% of sequence in fragments shorter than      80 bp
  20% of sequence in fragments shorter than      99 bp
  25% of sequence in fragments shorter than     117 bp
  30% of sequence in fragments shorter than     136 bp
  35% of sequence in fragments shorter than     156 bp
  40% of sequence in fragments shorter than     177 bp
  45% of sequence in fragments shorter than     200 bp
  50% of sequence in fragments shorter than     226 bp
  55% of sequence in fragments shorter than     252 bp
  60% of sequence in fragments shorter than     281 bp
  65% of sequence in fragments shorter than     312 bp
  70% of sequence in fragments shorter than     349 bp
  75% of sequence in fragments shorter than     393 bp
  80% of sequence in fragments shorter than     448 bp
  85% of sequence in fragments shorter than     522 bp
  90% of sequence in fragments shorter than     625 bp
  95% of sequence in fragments shorter than     826 bp

# DdeI.MboII (CTNAG+GAAGA) double digest, 23511 thousand fragments (14312045+9199496 sites), 126 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      67 bp
  15% of sequence in fragments shorter than      87 bp
  20% of sequence in fragments shorter than     107 bp
  25% of sequence in fragments shorter than     123 bp
  30% of sequence in fragments shorter than     139 bp
  35% of sequence in fragments shorter than     155 bp
  40% of sequence in fragments shorter than     173 bp
  45% of sequence in fragments shorter than     192 bp
  50% of sequence in fragments shorter than     214 bp
  55% of sequence in fragments shorter than     236 bp
  60% of sequence in fragments shorter than     260 bp
  65% of sequence in fragments shorter than     287 bp
  70% of sequence in fragments shorter than     316 bp
  75% of sequence in fragments shorter than     350 bp
  80% of sequence in fragments shorter than     393 bp
  85% of sequence in fragments shorter than     447 bp
  90% of sequence in fragments shorter than     522 bp
  95% of sequence in fragments shorter than     654 bp

# AciI.MseI (CCGC+TTAA) double digest, 23420 thousand fragments (4206130+19214674 sites), 127 bp average fragment length
   5% of sequence in fragments shorter than      41 bp
  10% of sequence in fragments shorter than      64 bp
  15% of sequence in fragments shorter than      84 bp
  20% of sequence in fragments shorter than     103 bp
  25% of sequence in fragments shorter than     124 bp
  30% of sequence in fragments shorter than     144 bp
  35% of sequence in fragments shorter than     163 bp
  40% of sequence in fragments shorter than     185 bp
  45% of sequence in fragments shorter than     208 bp
  50% of sequence in fragments shorter than     233 bp
  55% of sequence in fragments shorter than     260 bp
  60% of sequence in fragments shorter than     289 bp
  65% of sequence in fragments shorter than     322 bp
  70% of sequence in fragments shorter than     357 bp
  75% of sequence in fragments shorter than     400 bp
  80% of sequence in fragments shorter than     453 bp
  85% of sequence in fragments shorter than     524 bp
  90% of sequence in fragments shorter than     627 bp
  95% of sequence in fragments shorter than     815 bp

# DdeI.Hpy188I (CTNAG+TCNGA) double digest, 23253 thousand fragments (14312045+8942153 sites), 128 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     116 bp
  25% of sequence in fragments shorter than     134 bp
  30% of sequence in fragments shorter than     153 bp
  35% of sequence in fragments shorter than     172 bp
  40% of sequence in fragments shorter than     189 bp
  45% of sequence in fragments shorter than     211 bp
  50% of sequence in fragments shorter than     235 bp
  55% of sequence in fragments shorter than     260 bp
  60% of sequence in fragments shorter than     286 bp
  65% of sequence in fragments shorter than     316 bp
  70% of sequence in fragments shorter than     347 bp
  75% of sequence in fragments shorter than     387 bp
  80% of sequence in fragments shorter than     431 bp
  85% of sequence in fragments shorter than     491 bp
  90% of sequence in fragments shorter than     574 bp
  95% of sequence in fragments shorter than     712 bp

# MseI.Tsp45I (TTAA+GTSAC) double digest, 23235 thousand fragments (19214674+4021761 sites), 128 bp average fragment length
   5% of sequence in fragments shorter than      41 bp
  10% of sequence in fragments shorter than      63 bp
  15% of sequence in fragments shorter than      83 bp
  20% of sequence in fragments shorter than     103 bp
  25% of sequence in fragments shorter than     123 bp
  30% of sequence in fragments shorter than     143 bp
  35% of sequence in fragments shorter than     164 bp
  40% of sequence in fragments shorter than     187 bp
  45% of sequence in fragments shorter than     211 bp
  50% of sequence in fragments shorter than     238 bp
  55% of sequence in fragments shorter than     267 bp
  60% of sequence in fragments shorter than     296 bp
  65% of sequence in fragments shorter than     329 bp
  70% of sequence in fragments shorter than     364 bp
  75% of sequence in fragments shorter than     409 bp
  80% of sequence in fragments shorter than     465 bp
  85% of sequence in fragments shorter than     543 bp
  90% of sequence in fragments shorter than     657 bp
  95% of sequence in fragments shorter than     863 bp

# DdeI.HinfI (CTNAG+GANTC) double digest, 23227 thousand fragments (14312045+8916054 sites), 128 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than      88 bp
  20% of sequence in fragments shorter than     106 bp
  25% of sequence in fragments shorter than     123 bp
  30% of sequence in fragments shorter than     141 bp
  35% of sequence in fragments shorter than     161 bp
  40% of sequence in fragments shorter than     179 bp
  45% of sequence in fragments shorter than     201 bp
  50% of sequence in fragments shorter than     224 bp
  55% of sequence in fragments shorter than     248 bp
  60% of sequence in fragments shorter than     274 bp
  65% of sequence in fragments shorter than     302 bp
  70% of sequence in fragments shorter than     335 bp
  75% of sequence in fragments shorter than     373 bp
  80% of sequence in fragments shorter than     419 bp
  85% of sequence in fragments shorter than     479 bp
  90% of sequence in fragments shorter than     562 bp
  95% of sequence in fragments shorter than     703 bp

# MlyI.MseI (GAGTC+TTAA) double digest, 23145 thousand fragments (3931692+19214674 sites), 128 bp average fragment length
   5% of sequence in fragments shorter than      41 bp
  10% of sequence in fragments shorter than      64 bp
  15% of sequence in fragments shorter than      84 bp
  20% of sequence in fragments shorter than     104 bp
  25% of sequence in fragments shorter than     124 bp
  30% of sequence in fragments shorter than     144 bp
  35% of sequence in fragments shorter than     164 bp
  40% of sequence in fragments shorter than     187 bp
  45% of sequence in fragments shorter than     211 bp
  50% of sequence in fragments shorter than     238 bp
  55% of sequence in fragments shorter than     267 bp
  60% of sequence in fragments shorter than     293 bp
  65% of sequence in fragments shorter than     323 bp
  70% of sequence in fragments shorter than     358 bp
  75% of sequence in fragments shorter than     401 bp
  80% of sequence in fragments shorter than     456 bp
  85% of sequence in fragments shorter than     532 bp
  90% of sequence in fragments shorter than     645 bp
  95% of sequence in fragments shorter than     857 bp

# DdeI.TspRI (CTNAG+CASTG) double digest, 23076 thousand fragments (14312045+8765240 sites), 129 bp average fragment length
   5% of sequence in fragments shorter than      42 bp
  10% of sequence in fragments shorter than      66 bp
  15% of sequence in fragments shorter than      88 bp
  20% of sequence in fragments shorter than     109 bp
  25% of sequence in fragments shorter than     126 bp
  30% of sequence in fragments shorter than     144 bp
  35% of sequence in fragments shorter than     165 bp
  40% of sequence in fragments shorter than     181 bp
  45% of sequence in fragments shorter than     203 bp
  50% of sequence in fragments shorter than     227 bp
  55% of sequence in fragments shorter than     252 bp
  60% of sequence in fragments shorter than     280 bp
  65% of sequence in fragments shorter than     310 bp
  70% of sequence in fragments shorter than     345 bp
  75% of sequence in fragments shorter than     386 bp
  80% of sequence in fragments shorter than     437 bp
  85% of sequence in fragments shorter than     502 bp
  90% of sequence in fragments shorter than     590 bp
  95% of sequence in fragments shorter than     749 bp

# CviAII.MboII (CATG+GAAGA) double digest, 23014 thousand fragments (13815697+9199496 sites), 129 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      67 bp
  15% of sequence in fragments shorter than      87 bp
  20% of sequence in fragments shorter than     106 bp
  25% of sequence in fragments shorter than     125 bp
  30% of sequence in fragments shorter than     144 bp
  35% of sequence in fragments shorter than     161 bp
  40% of sequence in fragments shorter than     179 bp
  45% of sequence in fragments shorter than     199 bp
  50% of sequence in fragments shorter than     221 bp
  55% of sequence in fragments shorter than     244 bp
  60% of sequence in fragments shorter than     268 bp
  65% of sequence in fragments shorter than     293 bp
  70% of sequence in fragments shorter than     323 bp
  75% of sequence in fragments shorter than     356 bp
  80% of sequence in fragments shorter than     400 bp
  85% of sequence in fragments shorter than     452 bp
  90% of sequence in fragments shorter than     524 bp
  95% of sequence in fragments shorter than     650 bp

# AluI.BstNI (AGCT+CCWGG) double digest, 22882 thousand fragments (13027778+9855646 sites), 130 bp average fragment length
   5% of sequence in fragments shorter than      39 bp
  10% of sequence in fragments shorter than      60 bp
  15% of sequence in fragments shorter than      80 bp
  20% of sequence in fragments shorter than      98 bp
  25% of sequence in fragments shorter than     120 bp
  30% of sequence in fragments shorter than     141 bp
  35% of sequence in fragments shorter than     165 bp
  40% of sequence in fragments shorter than     189 bp
  45% of sequence in fragments shorter than     214 bp
  50% of sequence in fragments shorter than     240 bp
  55% of sequence in fragments shorter than     270 bp
  60% of sequence in fragments shorter than     304 bp
  65% of sequence in fragments shorter than     339 bp
  70% of sequence in fragments shorter than     380 bp
  75% of sequence in fragments shorter than     431 bp
  80% of sequence in fragments shorter than     492 bp
  85% of sequence in fragments shorter than     572 bp
  90% of sequence in fragments shorter than     690 bp
  95% of sequence in fragments shorter than     895 bp

# DdeI.HaeIII (CTNAG+GGCC) double digest, 22873 thousand fragments (14312045+8562230 sites), 130 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      64 bp
  15% of sequence in fragments shorter than      86 bp
  20% of sequence in fragments shorter than     106 bp
  25% of sequence in fragments shorter than     123 bp
  30% of sequence in fragments shorter than     142 bp
  35% of sequence in fragments shorter than     164 bp
  40% of sequence in fragments shorter than     184 bp
  45% of sequence in fragments shorter than     210 bp
  50% of sequence in fragments shorter than     236 bp
  55% of sequence in fragments shorter than     265 bp
  60% of sequence in fragments shorter than     295 bp
  65% of sequence in fragments shorter than     331 bp
  70% of sequence in fragments shorter than     372 bp
  75% of sequence in fragments shorter than     421 bp
  80% of sequence in fragments shorter than     479 bp
  85% of sequence in fragments shorter than     552 bp
  90% of sequence in fragments shorter than     658 bp
  95% of sequence in fragments shorter than     840 bp

# BscAI.MseI (GCATC+TTAA) double digest, 22867 thousand fragments (3652926+19214674 sites), 130 bp average fragment length
   5% of sequence in fragments shorter than      41 bp
  10% of sequence in fragments shorter than      64 bp
  15% of sequence in fragments shorter than      84 bp
  20% of sequence in fragments shorter than     104 bp
  25% of sequence in fragments shorter than     125 bp
  30% of sequence in fragments shorter than     145 bp
  35% of sequence in fragments shorter than     167 bp
  40% of sequence in fragments shorter than     189 bp
  45% of sequence in fragments shorter than     214 bp
  50% of sequence in fragments shorter than     242 bp
  55% of sequence in fragments shorter than     273 bp
  60% of sequence in fragments shorter than     307 bp
  65% of sequence in fragments shorter than     342 bp
  70% of sequence in fragments shorter than     381 bp
  75% of sequence in fragments shorter than     429 bp
  80% of sequence in fragments shorter than     489 bp
  85% of sequence in fragments shorter than     571 bp
  90% of sequence in fragments shorter than     690 bp
  95% of sequence in fragments shorter than     929 bp

# CviAII.Hpy188I (CATG+TCNGA) double digest, 22757 thousand fragments (13815697+8942153 sites), 130 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      66 bp
  15% of sequence in fragments shorter than      86 bp
  20% of sequence in fragments shorter than     105 bp
  25% of sequence in fragments shorter than     123 bp
  30% of sequence in fragments shorter than     141 bp
  35% of sequence in fragments shorter than     159 bp
  40% of sequence in fragments shorter than     176 bp
  45% of sequence in fragments shorter than     196 bp
  50% of sequence in fragments shorter than     218 bp
  55% of sequence in fragments shorter than     239 bp
  60% of sequence in fragments shorter than     262 bp
  65% of sequence in fragments shorter than     288 bp
  70% of sequence in fragments shorter than     317 bp
  75% of sequence in fragments shorter than     351 bp
  80% of sequence in fragments shorter than     392 bp
  85% of sequence in fragments shorter than     445 bp
  90% of sequence in fragments shorter than     517 bp
  95% of sequence in fragments shorter than     640 bp

# MseI.SfeI (TTAA+CTRYAG) double digest, 22745 thousand fragments (19214674+3531009 sites), 131 bp average fragment length
   5% of sequence in fragments shorter than      42 bp
  10% of sequence in fragments shorter than      64 bp
  15% of sequence in fragments shorter than      85 bp
  20% of sequence in fragments shorter than     105 bp
  25% of sequence in fragments shorter than     126 bp
  30% of sequence in fragments shorter than     146 bp
  35% of sequence in fragments shorter than     167 bp
  40% of sequence in fragments shorter than     188 bp
  45% of sequence in fragments shorter than     211 bp
  50% of sequence in fragments shorter than     238 bp
  55% of sequence in fragments shorter than     267 bp
  60% of sequence in fragments shorter than     298 bp
  65% of sequence in fragments shorter than     332 bp
  70% of sequence in fragments shorter than     370 bp
  75% of sequence in fragments shorter than     418 bp
  80% of sequence in fragments shorter than     479 bp
  85% of sequence in fragments shorter than     559 bp
  90% of sequence in fragments shorter than     679 bp
  95% of sequence in fragments shorter than     908 bp

# CviAII.HinfI (CATG+GANTC) double digest, 22731 thousand fragments (13815697+8916054 sites), 131 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      68 bp
  15% of sequence in fragments shorter than      86 bp
  20% of sequence in fragments shorter than     105 bp
  25% of sequence in fragments shorter than     124 bp
  30% of sequence in fragments shorter than     141 bp
  35% of sequence in fragments shorter than     161 bp
  40% of sequence in fragments shorter than     178 bp
  45% of sequence in fragments shorter than     198 bp
  50% of sequence in fragments shorter than     220 bp
  55% of sequence in fragments shorter than     244 bp
  60% of sequence in fragments shorter than     269 bp
  65% of sequence in fragments shorter than     297 bp
  70% of sequence in fragments shorter than     328 bp
  75% of sequence in fragments shorter than     360 bp
  80% of sequence in fragments shorter than     404 bp
  85% of sequence in fragments shorter than     460 bp
  90% of sequence in fragments shorter than     538 bp
  95% of sequence in fragments shorter than     673 bp

# CviAII.TspRI (CATG+CASTG) double digest, 22580 thousand fragments (13815697+8765240 sites), 131 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than      86 bp
  20% of sequence in fragments shorter than     105 bp
  25% of sequence in fragments shorter than     125 bp
  30% of sequence in fragments shorter than     141 bp
  35% of sequence in fragments shorter than     160 bp
  40% of sequence in fragments shorter than     181 bp
  45% of sequence in fragments shorter than     203 bp
  50% of sequence in fragments shorter than     225 bp
  55% of sequence in fragments shorter than     249 bp
  60% of sequence in fragments shorter than     276 bp
  65% of sequence in fragments shorter than     306 bp
  70% of sequence in fragments shorter than     339 bp
  75% of sequence in fragments shorter than     379 bp
  80% of sequence in fragments shorter than     429 bp
  85% of sequence in fragments shorter than     493 bp
  90% of sequence in fragments shorter than     585 bp
  95% of sequence in fragments shorter than     753 bp

# CviAII.HaeIII (CATG+GGCC) double digest, 22377 thousand fragments (13815697+8562230 sites), 133 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      65 bp
  15% of sequence in fragments shorter than      88 bp
  20% of sequence in fragments shorter than     109 bp
  25% of sequence in fragments shorter than     132 bp
  30% of sequence in fragments shorter than     153 bp
  35% of sequence in fragments shorter than     175 bp
  40% of sequence in fragments shorter than     199 bp
  45% of sequence in fragments shorter than     223 bp
  50% of sequence in fragments shorter than     249 bp
  55% of sequence in fragments shorter than     277 bp
  60% of sequence in fragments shorter than     306 bp
  65% of sequence in fragments shorter than     338 bp
  70% of sequence in fragments shorter than     372 bp
  75% of sequence in fragments shorter than     417 bp
  80% of sequence in fragments shorter than     470 bp
  85% of sequence in fragments shorter than     538 bp
  90% of sequence in fragments shorter than     635 bp
  95% of sequence in fragments shorter than     797 bp

# MseI.StyI (TTAA+CCWWGG) double digest, 22328 thousand fragments (19214674+3114107 sites), 133 bp average fragment length
   5% of sequence in fragments shorter than      42 bp
  10% of sequence in fragments shorter than      66 bp
  15% of sequence in fragments shorter than      86 bp
  20% of sequence in fragments shorter than     106 bp
  25% of sequence in fragments shorter than     128 bp
  30% of sequence in fragments shorter than     149 bp
  35% of sequence in fragments shorter than     170 bp
  40% of sequence in fragments shorter than     194 bp
  45% of sequence in fragments shorter than     220 bp
  50% of sequence in fragments shorter than     248 bp
  55% of sequence in fragments shorter than     277 bp
  60% of sequence in fragments shorter than     308 bp
  65% of sequence in fragments shorter than     342 bp
  70% of sequence in fragments shorter than     379 bp
  75% of sequence in fragments shorter than     427 bp
  80% of sequence in fragments shorter than     485 bp
  85% of sequence in fragments shorter than     567 bp
  90% of sequence in fragments shorter than     685 bp
  95% of sequence in fragments shorter than     917 bp

# MseI.NspI (TTAA+RCATGY) double digest, 22318 thousand fragments (19214674+3104359 sites), 133 bp average fragment length
   5% of sequence in fragments shorter than      41 bp
  10% of sequence in fragments shorter than      65 bp
  15% of sequence in fragments shorter than      87 bp
  20% of sequence in fragments shorter than     108 bp
  25% of sequence in fragments shorter than     130 bp
  30% of sequence in fragments shorter than     151 bp
  35% of sequence in fragments shorter than     173 bp
  40% of sequence in fragments shorter than     197 bp
  45% of sequence in fragments shorter than     223 bp
  50% of sequence in fragments shorter than     252 bp
  55% of sequence in fragments shorter than     286 bp
  60% of sequence in fragments shorter than     321 bp
  65% of sequence in fragments shorter than     358 bp
  70% of sequence in fragments shorter than     403 bp
  75% of sequence in fragments shorter than     458 bp
  80% of sequence in fragments shorter than     529 bp
  85% of sequence in fragments shorter than     628 bp
  90% of sequence in fragments shorter than     777 bp
  95% of sequence in fragments shorter than    1099 bp

# AluI.MboII (AGCT+GAAGA) double digest, 22226 thousand fragments (13027778+9199496 sites), 134 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than      90 bp
  20% of sequence in fragments shorter than     110 bp
  25% of sequence in fragments shorter than     130 bp
  30% of sequence in fragments shorter than     149 bp
  35% of sequence in fragments shorter than     168 bp
  40% of sequence in fragments shorter than     186 bp
  45% of sequence in fragments shorter than     205 bp
  50% of sequence in fragments shorter than     227 bp
  55% of sequence in fragments shorter than     250 bp
  60% of sequence in fragments shorter than     274 bp
  65% of sequence in fragments shorter than     302 bp
  70% of sequence in fragments shorter than     333 bp
  75% of sequence in fragments shorter than     368 bp
  80% of sequence in fragments shorter than     412 bp
  85% of sequence in fragments shorter than     467 bp
  90% of sequence in fragments shorter than     545 bp
  95% of sequence in fragments shorter than     682 bp

# AluI.Hpy188I (AGCT+TCNGA) double digest, 21969 thousand fragments (13027778+8942153 sites), 135 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than      91 bp
  20% of sequence in fragments shorter than     110 bp
  25% of sequence in fragments shorter than     130 bp
  30% of sequence in fragments shorter than     150 bp
  35% of sequence in fragments shorter than     169 bp
  40% of sequence in fragments shorter than     187 bp
  45% of sequence in fragments shorter than     208 bp
  50% of sequence in fragments shorter than     229 bp
  55% of sequence in fragments shorter than     252 bp
  60% of sequence in fragments shorter than     278 bp
  65% of sequence in fragments shorter than     307 bp
  70% of sequence in fragments shorter than     337 bp
  75% of sequence in fragments shorter than     373 bp
  80% of sequence in fragments shorter than     418 bp
  85% of sequence in fragments shorter than     474 bp
  90% of sequence in fragments shorter than     555 bp
  95% of sequence in fragments shorter than     696 bp

# AvaII.MseI (GGWCC+TTAA) double digest, 21961 thousand fragments (2747690+19214674 sites), 135 bp average fragment length
   5% of sequence in fragments shorter than      43 bp
  10% of sequence in fragments shorter than      67 bp
  15% of sequence in fragments shorter than      89 bp
  20% of sequence in fragments shorter than     110 bp
  25% of sequence in fragments shorter than     131 bp
  30% of sequence in fragments shorter than     152 bp
  35% of sequence in fragments shorter than     174 bp
  40% of sequence in fragments shorter than     198 bp
  45% of sequence in fragments shorter than     224 bp
  50% of sequence in fragments shorter than     252 bp
  55% of sequence in fragments shorter than     282 bp
  60% of sequence in fragments shorter than     315 bp
  65% of sequence in fragments shorter than     348 bp
  70% of sequence in fragments shorter than     388 bp
  75% of sequence in fragments shorter than     436 bp
  80% of sequence in fragments shorter than     494 bp
  85% of sequence in fragments shorter than     572 bp
  90% of sequence in fragments shorter than     685 bp
  95% of sequence in fragments shorter than     887 bp

# AluI.HinfI (AGCT+GANTC) double digest, 21943 thousand fragments (13027778+8916054 sites), 135 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      67 bp
  15% of sequence in fragments shorter than      88 bp
  20% of sequence in fragments shorter than     108 bp
  25% of sequence in fragments shorter than     128 bp
  30% of sequence in fragments shorter than     148 bp
  35% of sequence in fragments shorter than     168 bp
  40% of sequence in fragments shorter than     189 bp
  45% of sequence in fragments shorter than     209 bp
  50% of sequence in fragments shorter than     231 bp
  55% of sequence in fragments shorter than     256 bp
  60% of sequence in fragments shorter than     281 bp
  65% of sequence in fragments shorter than     311 bp
  70% of sequence in fragments shorter than     342 bp
  75% of sequence in fragments shorter than     377 bp
  80% of sequence in fragments shorter than     424 bp
  85% of sequence in fragments shorter than     483 bp
  90% of sequence in fragments shorter than     567 bp
  95% of sequence in fragments shorter than     707 bp

# MseI.SmlI (TTAA+CTYRAG) double digest, 21942 thousand fragments (19214674+2727867 sites), 135 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than      89 bp
  20% of sequence in fragments shorter than     111 bp
  25% of sequence in fragments shorter than     133 bp
  30% of sequence in fragments shorter than     155 bp
  35% of sequence in fragments shorter than     180 bp
  40% of sequence in fragments shorter than     206 bp
  45% of sequence in fragments shorter than     234 bp
  50% of sequence in fragments shorter than     265 bp
  55% of sequence in fragments shorter than     297 bp
  60% of sequence in fragments shorter than     332 bp
  65% of sequence in fragments shorter than     369 bp
  70% of sequence in fragments shorter than     416 bp
  75% of sequence in fragments shorter than     472 bp
  80% of sequence in fragments shorter than     545 bp
  85% of sequence in fragments shorter than     645 bp
  90% of sequence in fragments shorter than     797 bp
  95% of sequence in fragments shorter than    1134 bp

# AluI.TspRI (AGCT+CASTG) double digest, 21792 thousand fragments (13027778+8765240 sites), 136 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      67 bp
  15% of sequence in fragments shorter than      90 bp
  20% of sequence in fragments shorter than     111 bp
  25% of sequence in fragments shorter than     131 bp
  30% of sequence in fragments shorter than     152 bp
  35% of sequence in fragments shorter than     172 bp
  40% of sequence in fragments shorter than     194 bp
  45% of sequence in fragments shorter than     216 bp
  50% of sequence in fragments shorter than     239 bp
  55% of sequence in fragments shorter than     264 bp
  60% of sequence in fragments shorter than     291 bp
  65% of sequence in fragments shorter than     323 bp
  70% of sequence in fragments shorter than     357 bp
  75% of sequence in fragments shorter than     399 bp
  80% of sequence in fragments shorter than     449 bp
  85% of sequence in fragments shorter than     514 bp
  90% of sequence in fragments shorter than     606 bp
  95% of sequence in fragments shorter than     768 bp

# BspNCI.DdeI (CCAGA+CTNAG) double digest, 21593 thousand fragments (7282436+14312045 sites), 138 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      92 bp
  20% of sequence in fragments shorter than     112 bp
  25% of sequence in fragments shorter than     130 bp
  30% of sequence in fragments shorter than     148 bp
  35% of sequence in fragments shorter than     169 bp
  40% of sequence in fragments shorter than     186 bp
  45% of sequence in fragments shorter than     209 bp
  50% of sequence in fragments shorter than     233 bp
  55% of sequence in fragments shorter than     259 bp
  60% of sequence in fragments shorter than     287 bp
  65% of sequence in fragments shorter than     318 bp
  70% of sequence in fragments shorter than     352 bp
  75% of sequence in fragments shorter than     394 bp
  80% of sequence in fragments shorter than     441 bp
  85% of sequence in fragments shorter than     506 bp
  90% of sequence in fragments shorter than     589 bp
  95% of sequence in fragments shorter than     740 bp

# MseI.SspI (TTAA+AATATT) double digest, 21591 thousand fragments (19214674+2377267 sites), 138 bp average fragment length
   5% of sequence in fragments shorter than      42 bp
  10% of sequence in fragments shorter than      68 bp
  15% of sequence in fragments shorter than      90 bp
  20% of sequence in fragments shorter than     114 bp
  25% of sequence in fragments shorter than     138 bp
  30% of sequence in fragments shorter than     162 bp
  35% of sequence in fragments shorter than     189 bp
  40% of sequence in fragments shorter than     219 bp
  45% of sequence in fragments shorter than     251 bp
  50% of sequence in fragments shorter than     287 bp
  55% of sequence in fragments shorter than     323 bp
  60% of sequence in fragments shorter than     361 bp
  65% of sequence in fragments shorter than     407 bp
  70% of sequence in fragments shorter than     462 bp
  75% of sequence in fragments shorter than     532 bp
  80% of sequence in fragments shorter than     624 bp
  85% of sequence in fragments shorter than     747 bp
  90% of sequence in fragments shorter than     956 bp
  95% of sequence in fragments shorter than    1410 bp

# AluI.HaeIII (AGCT+GGCC) double digest, 21589 thousand fragments (13027778+8562230 sites), 138 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      66 bp
  15% of sequence in fragments shorter than      87 bp
  20% of sequence in fragments shorter than     109 bp
  25% of sequence in fragments shorter than     129 bp
  30% of sequence in fragments shorter than     151 bp
  35% of sequence in fragments shorter than     173 bp
  40% of sequence in fragments shorter than     196 bp
  45% of sequence in fragments shorter than     221 bp
  50% of sequence in fragments shorter than     248 bp
  55% of sequence in fragments shorter than     278 bp
  60% of sequence in fragments shorter than     310 bp
  65% of sequence in fragments shorter than     345 bp
  70% of sequence in fragments shorter than     384 bp
  75% of sequence in fragments shorter than     433 bp
  80% of sequence in fragments shorter than     490 bp
  85% of sequence in fragments shorter than     564 bp
  90% of sequence in fragments shorter than     672 bp
  95% of sequence in fragments shorter than     857 bp

# HpaII.MseI (CCGG+TTAA) double digest, 21531 thousand fragments (2317722+19214674 sites), 138 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than      91 bp
  20% of sequence in fragments shorter than     113 bp
  25% of sequence in fragments shorter than     135 bp
  30% of sequence in fragments shorter than     155 bp
  35% of sequence in fragments shorter than     178 bp
  40% of sequence in fragments shorter than     202 bp
  45% of sequence in fragments shorter than     227 bp
  50% of sequence in fragments shorter than     255 bp
  55% of sequence in fragments shorter than     286 bp
  60% of sequence in fragments shorter than     318 bp
  65% of sequence in fragments shorter than     351 bp
  70% of sequence in fragments shorter than     392 bp
  75% of sequence in fragments shorter than     441 bp
  80% of sequence in fragments shorter than     502 bp
  85% of sequence in fragments shorter than     585 bp
  90% of sequence in fragments shorter than     699 bp
  95% of sequence in fragments shorter than     930 bp

# BspKT6I.DdeI (GATC+CTNAG) double digest, 21510 thousand fragments (7199434+14312045 sites), 138 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     115 bp
  25% of sequence in fragments shorter than     131 bp
  30% of sequence in fragments shorter than     151 bp
  35% of sequence in fragments shorter than     172 bp
  40% of sequence in fragments shorter than     189 bp
  45% of sequence in fragments shorter than     213 bp
  50% of sequence in fragments shorter than     237 bp
  55% of sequence in fragments shorter than     264 bp
  60% of sequence in fragments shorter than     291 bp
  65% of sequence in fragments shorter than     323 bp
  70% of sequence in fragments shorter than     356 bp
  75% of sequence in fragments shorter than     399 bp
  80% of sequence in fragments shorter than     447 bp
  85% of sequence in fragments shorter than     506 bp
  90% of sequence in fragments shorter than     594 bp
  95% of sequence in fragments shorter than     746 bp

# DdeI.Hin4II (CTNAG+CCTTC) double digest, 21371 thousand fragments (14312045+7059915 sites), 139 bp average fragment length
   5% of sequence in fragments shorter than      48 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     116 bp
  25% of sequence in fragments shorter than     134 bp
  30% of sequence in fragments shorter than     153 bp
  35% of sequence in fragments shorter than     173 bp
  40% of sequence in fragments shorter than     193 bp
  45% of sequence in fragments shorter than     217 bp
  50% of sequence in fragments shorter than     241 bp
  55% of sequence in fragments shorter than     267 bp
  60% of sequence in fragments shorter than     295 bp
  65% of sequence in fragments shorter than     326 bp
  70% of sequence in fragments shorter than     361 bp
  75% of sequence in fragments shorter than     403 bp
  80% of sequence in fragments shorter than     454 bp
  85% of sequence in fragments shorter than     521 bp
  90% of sequence in fragments shorter than     614 bp
  95% of sequence in fragments shorter than     777 bp

# BspNCI.CviAII (CCAGA+CATG) double digest, 21097 thousand fragments (7282436+13815697 sites), 141 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      92 bp
  20% of sequence in fragments shorter than     113 bp
  25% of sequence in fragments shorter than     133 bp
  30% of sequence in fragments shorter than     153 bp
  35% of sequence in fragments shorter than     173 bp
  40% of sequence in fragments shorter than     195 bp
  45% of sequence in fragments shorter than     218 bp
  50% of sequence in fragments shorter than     241 bp
  55% of sequence in fragments shorter than     266 bp
  60% of sequence in fragments shorter than     293 bp
  65% of sequence in fragments shorter than     323 bp
  70% of sequence in fragments shorter than     356 bp
  75% of sequence in fragments shorter than     396 bp
  80% of sequence in fragments shorter than     445 bp
  85% of sequence in fragments shorter than     508 bp
  90% of sequence in fragments shorter than     594 bp
  95% of sequence in fragments shorter than     753 bp

# BspKT6I.CviAII (GATC+CATG) double digest, 21014 thousand fragments (7199434+13815697 sites), 141 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      94 bp
  20% of sequence in fragments shorter than     116 bp
  25% of sequence in fragments shorter than     137 bp
  30% of sequence in fragments shorter than     156 bp
  35% of sequence in fragments shorter than     177 bp
  40% of sequence in fragments shorter than     199 bp
  45% of sequence in fragments shorter than     223 bp
  50% of sequence in fragments shorter than     247 bp
  55% of sequence in fragments shorter than     274 bp
  60% of sequence in fragments shorter than     301 bp
  65% of sequence in fragments shorter than     333 bp
  70% of sequence in fragments shorter than     369 bp
  75% of sequence in fragments shorter than     412 bp
  80% of sequence in fragments shorter than     462 bp
  85% of sequence in fragments shorter than     528 bp
  90% of sequence in fragments shorter than     620 bp
  95% of sequence in fragments shorter than     790 bp

# MboII.ScrFI (GAAGA+CCNGG) double digest, 21004 thousand fragments (9199496+11805099 sites), 141 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than      90 bp
  20% of sequence in fragments shorter than     115 bp
  25% of sequence in fragments shorter than     134 bp
  30% of sequence in fragments shorter than     155 bp
  35% of sequence in fragments shorter than     176 bp
  40% of sequence in fragments shorter than     202 bp
  45% of sequence in fragments shorter than     228 bp
  50% of sequence in fragments shorter than     257 bp
  55% of sequence in fragments shorter than     289 bp
  60% of sequence in fragments shorter than     322 bp
  65% of sequence in fragments shorter than     357 bp
  70% of sequence in fragments shorter than     402 bp
  75% of sequence in fragments shorter than     451 bp
  80% of sequence in fragments shorter than     514 bp
  85% of sequence in fragments shorter than     593 bp
  90% of sequence in fragments shorter than     702 bp
  95% of sequence in fragments shorter than     897 bp

# HhaI.MseI (GCGC+TTAA) double digest, 20970 thousand fragments (1756502+19214674 sites), 142 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      94 bp
  20% of sequence in fragments shorter than     117 bp
  25% of sequence in fragments shorter than     139 bp
  30% of sequence in fragments shorter than     161 bp
  35% of sequence in fragments shorter than     185 bp
  40% of sequence in fragments shorter than     211 bp
  45% of sequence in fragments shorter than     239 bp
  50% of sequence in fragments shorter than     267 bp
  55% of sequence in fragments shorter than     299 bp
  60% of sequence in fragments shorter than     333 bp
  65% of sequence in fragments shorter than     368 bp
  70% of sequence in fragments shorter than     413 bp
  75% of sequence in fragments shorter than     466 bp
  80% of sequence in fragments shorter than     534 bp
  85% of sequence in fragments shorter than     624 bp
  90% of sequence in fragments shorter than     745 bp
  95% of sequence in fragments shorter than     989 bp

# BsmAI.DdeI (GTCTC+CTNAG) double digest, 20943 thousand fragments (6631585+14312045 sites), 142 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      94 bp
  20% of sequence in fragments shorter than     114 bp
  25% of sequence in fragments shorter than     137 bp
  30% of sequence in fragments shorter than     161 bp
  35% of sequence in fragments shorter than     180 bp
  40% of sequence in fragments shorter than     205 bp
  45% of sequence in fragments shorter than     231 bp
  50% of sequence in fragments shorter than     260 bp
  55% of sequence in fragments shorter than     289 bp
  60% of sequence in fragments shorter than     322 bp
  65% of sequence in fragments shorter than     357 bp
  70% of sequence in fragments shorter than     399 bp
  75% of sequence in fragments shorter than     447 bp
  80% of sequence in fragments shorter than     507 bp
  85% of sequence in fragments shorter than     579 bp
  90% of sequence in fragments shorter than     685 bp
  95% of sequence in fragments shorter than     866 bp

# BfiI.MseI (ACTGGG+TTAA) double digest, 20920 thousand fragments (1706594+19214674 sites), 142 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      70 bp
  15% of sequence in fragments shorter than      93 bp
  20% of sequence in fragments shorter than     115 bp
  25% of sequence in fragments shorter than     137 bp
  30% of sequence in fragments shorter than     160 bp
  35% of sequence in fragments shorter than     185 bp
  40% of sequence in fragments shorter than     211 bp
  45% of sequence in fragments shorter than     240 bp
  50% of sequence in fragments shorter than     272 bp
  55% of sequence in fragments shorter than     306 bp
  60% of sequence in fragments shorter than     342 bp
  65% of sequence in fragments shorter than     379 bp
  70% of sequence in fragments shorter than     426 bp
  75% of sequence in fragments shorter than     481 bp
  80% of sequence in fragments shorter than     553 bp
  85% of sequence in fragments shorter than     651 bp
  90% of sequence in fragments shorter than     797 bp
  95% of sequence in fragments shorter than    1101 bp

# CviAII.Hin4II (CATG+CCTTC) double digest, 20875 thousand fragments (13815697+7059915 sites), 142 bp average fragment length
   5% of sequence in fragments shorter than      48 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      95 bp
  20% of sequence in fragments shorter than     117 bp
  25% of sequence in fragments shorter than     138 bp
  30% of sequence in fragments shorter than     159 bp
  35% of sequence in fragments shorter than     181 bp
  40% of sequence in fragments shorter than     203 bp
  45% of sequence in fragments shorter than     226 bp
  50% of sequence in fragments shorter than     250 bp
  55% of sequence in fragments shorter than     275 bp
  60% of sequence in fragments shorter than     303 bp
  65% of sequence in fragments shorter than     334 bp
  70% of sequence in fragments shorter than     368 bp
  75% of sequence in fragments shorter than     410 bp
  80% of sequence in fragments shorter than     460 bp
  85% of sequence in fragments shorter than     524 bp
  90% of sequence in fragments shorter than     615 bp
  95% of sequence in fragments shorter than     769 bp

# MseI.PsiI (TTAA+TTATAA) double digest, 20862 thousand fragments (19214674+1647912 sites), 142 bp average fragment length
   5% of sequence in fragments shorter than      43 bp
  10% of sequence in fragments shorter than      68 bp
  15% of sequence in fragments shorter than      92 bp
  20% of sequence in fragments shorter than     115 bp
  25% of sequence in fragments shorter than     139 bp
  30% of sequence in fragments shorter than     163 bp
  35% of sequence in fragments shorter than     189 bp
  40% of sequence in fragments shorter than     219 bp
  45% of sequence in fragments shorter than     251 bp
  50% of sequence in fragments shorter than     287 bp
  55% of sequence in fragments shorter than     325 bp
  60% of sequence in fragments shorter than     362 bp
  65% of sequence in fragments shorter than     408 bp
  70% of sequence in fragments shorter than     463 bp
  75% of sequence in fragments shorter than     533 bp
  80% of sequence in fragments shorter than     627 bp
  85% of sequence in fragments shorter than     753 bp
  90% of sequence in fragments shorter than     971 bp
  95% of sequence in fragments shorter than    1459 bp

# EsaBC3I.MseI (TCGA+TTAA) double digest, 20817 thousand fragments (1603346+19214674 sites), 143 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      93 bp
  20% of sequence in fragments shorter than     115 bp
  25% of sequence in fragments shorter than     138 bp
  30% of sequence in fragments shorter than     161 bp
  35% of sequence in fragments shorter than     187 bp
  40% of sequence in fragments shorter than     214 bp
  45% of sequence in fragments shorter than     242 bp
  50% of sequence in fragments shorter than     272 bp
  55% of sequence in fragments shorter than     304 bp
  60% of sequence in fragments shorter than     340 bp
  65% of sequence in fragments shorter than     378 bp
  70% of sequence in fragments shorter than     426 bp
  75% of sequence in fragments shorter than     482 bp
  80% of sequence in fragments shorter than     556 bp
  85% of sequence in fragments shorter than     654 bp
  90% of sequence in fragments shorter than     799 bp
  95% of sequence in fragments shorter than    1109 bp

# HgiCI.MseI (GGYRCC+TTAA) double digest, 20765 thousand fragments (1550877+19214674 sites), 143 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      94 bp
  20% of sequence in fragments shorter than     118 bp
  25% of sequence in fragments shorter than     140 bp
  30% of sequence in fragments shorter than     162 bp
  35% of sequence in fragments shorter than     184 bp
  40% of sequence in fragments shorter than     210 bp
  45% of sequence in fragments shorter than     238 bp
  50% of sequence in fragments shorter than     268 bp
  55% of sequence in fragments shorter than     300 bp
  60% of sequence in fragments shorter than     334 bp
  65% of sequence in fragments shorter than     370 bp
  70% of sequence in fragments shorter than     415 bp
  75% of sequence in fragments shorter than     468 bp
  80% of sequence in fragments shorter than     535 bp
  85% of sequence in fragments shorter than     625 bp
  90% of sequence in fragments shorter than     750 bp
  95% of sequence in fragments shorter than    1019 bp

# BsmI.MseI (GAATGC+TTAA) double digest, 20763 thousand fragments (1549350+19214674 sites), 143 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      70 bp
  15% of sequence in fragments shorter than      93 bp
  20% of sequence in fragments shorter than     117 bp
  25% of sequence in fragments shorter than     140 bp
  30% of sequence in fragments shorter than     162 bp
  35% of sequence in fragments shorter than     186 bp
  40% of sequence in fragments shorter than     213 bp
  45% of sequence in fragments shorter than     244 bp
  50% of sequence in fragments shorter than     277 bp
  55% of sequence in fragments shorter than     311 bp
  60% of sequence in fragments shorter than     345 bp
  65% of sequence in fragments shorter than     386 bp
  70% of sequence in fragments shorter than     436 bp
  75% of sequence in fragments shorter than     496 bp
  80% of sequence in fragments shorter than     574 bp
  85% of sequence in fragments shorter than     678 bp
  90% of sequence in fragments shorter than     839 bp
  95% of sequence in fragments shorter than    1170 bp

# Hpy188I.ScrFI (TCNGA+CCNGG) double digest, 20746 thousand fragments (8942153+11805099 sites), 143 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      68 bp
  15% of sequence in fragments shorter than      88 bp
  20% of sequence in fragments shorter than     113 bp
  25% of sequence in fragments shorter than     133 bp
  30% of sequence in fragments shorter than     156 bp
  35% of sequence in fragments shorter than     176 bp
  40% of sequence in fragments shorter than     202 bp
  45% of sequence in fragments shorter than     229 bp
  50% of sequence in fragments shorter than     258 bp
  55% of sequence in fragments shorter than     290 bp
  60% of sequence in fragments shorter than     326 bp
  65% of sequence in fragments shorter than     361 bp
  70% of sequence in fragments shorter than     406 bp
  75% of sequence in fragments shorter than     460 bp
  80% of sequence in fragments shorter than     526 bp
  85% of sequence in fragments shorter than     608 bp
  90% of sequence in fragments shorter than     723 bp
  95% of sequence in fragments shorter than     922 bp

# HinfI.ScrFI (GANTC+CCNGG) double digest, 20720 thousand fragments (8916054+11805099 sites), 143 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      68 bp
  15% of sequence in fragments shorter than      91 bp
  20% of sequence in fragments shorter than     116 bp
  25% of sequence in fragments shorter than     138 bp
  30% of sequence in fragments shorter than     165 bp
  35% of sequence in fragments shorter than     191 bp
  40% of sequence in fragments shorter than     220 bp
  45% of sequence in fragments shorter than     251 bp
  50% of sequence in fragments shorter than     283 bp
  55% of sequence in fragments shorter than     319 bp
  60% of sequence in fragments shorter than     349 bp
  65% of sequence in fragments shorter than     392 bp
  70% of sequence in fragments shorter than     443 bp
  75% of sequence in fragments shorter than     503 bp
  80% of sequence in fragments shorter than     576 bp
  85% of sequence in fragments shorter than     672 bp
  90% of sequence in fragments shorter than     797 bp
  95% of sequence in fragments shorter than    1020 bp

# AflIII.MseI (ACRYGT+TTAA) double digest, 20699 thousand fragments (1485394+19214674 sites), 143 bp average fragment length
   5% of sequence in fragments shorter than      44 bp
  10% of sequence in fragments shorter than      70 bp
  15% of sequence in fragments shorter than      93 bp
  20% of sequence in fragments shorter than     117 bp
  25% of sequence in fragments shorter than     140 bp
  30% of sequence in fragments shorter than     164 bp
  35% of sequence in fragments shorter than     191 bp
  40% of sequence in fragments shorter than     219 bp
  45% of sequence in fragments shorter than     250 bp
  50% of sequence in fragments shorter than     285 bp
  55% of sequence in fragments shorter than     321 bp
  60% of sequence in fragments shorter than     357 bp
  65% of sequence in fragments shorter than     401 bp
  70% of sequence in fragments shorter than     454 bp
  75% of sequence in fragments shorter than     519 bp
  80% of sequence in fragments shorter than     605 bp
  85% of sequence in fragments shorter than     718 bp
  90% of sequence in fragments shorter than     904 bp
  95% of sequence in fragments shorter than    1294 bp

# ApoI.DdeI (RAATTY+CTNAG) double digest, 20693 thousand fragments (6382433+14312045 sites), 144 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      76 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     118 bp
  25% of sequence in fragments shorter than     136 bp
  30% of sequence in fragments shorter than     154 bp
  35% of sequence in fragments shorter than     173 bp
  40% of sequence in fragments shorter than     192 bp
  45% of sequence in fragments shorter than     213 bp
  50% of sequence in fragments shorter than     236 bp
  55% of sequence in fragments shorter than     260 bp
  60% of sequence in fragments shorter than     286 bp
  65% of sequence in fragments shorter than     314 bp
  70% of sequence in fragments shorter than     346 bp
  75% of sequence in fragments shorter than     381 bp
  80% of sequence in fragments shorter than     424 bp
  85% of sequence in fragments shorter than     482 bp
  90% of sequence in fragments shorter than     561 bp
  95% of sequence in fragments shorter than     694 bp

# FauI.MseI (CCCGC+TTAA) double digest, 20653 thousand fragments (1439772+19214674 sites), 144 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      94 bp
  20% of sequence in fragments shorter than     117 bp
  25% of sequence in fragments shorter than     140 bp
  30% of sequence in fragments shorter than     164 bp
  35% of sequence in fragments shorter than     188 bp
  40% of sequence in fragments shorter than     215 bp
  45% of sequence in fragments shorter than     244 bp
  50% of sequence in fragments shorter than     275 bp
  55% of sequence in fragments shorter than     306 bp
  60% of sequence in fragments shorter than     341 bp
  65% of sequence in fragments shorter than     378 bp
  70% of sequence in fragments shorter than     423 bp
  75% of sequence in fragments shorter than     477 bp
  80% of sequence in fragments shorter than     547 bp
  85% of sequence in fragments shorter than     638 bp
  90% of sequence in fragments shorter than     771 bp
  95% of sequence in fragments shorter than    1034 bp

# BspMI.MseI (ACCTGC+TTAA) double digest, 20652 thousand fragments (1438129+19214674 sites), 144 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      94 bp
  20% of sequence in fragments shorter than     117 bp
  25% of sequence in fragments shorter than     141 bp
  30% of sequence in fragments shorter than     164 bp
  35% of sequence in fragments shorter than     189 bp
  40% of sequence in fragments shorter than     217 bp
  45% of sequence in fragments shorter than     247 bp
  50% of sequence in fragments shorter than     279 bp
  55% of sequence in fragments shorter than     311 bp
  60% of sequence in fragments shorter than     345 bp
  65% of sequence in fragments shorter than     382 bp
  70% of sequence in fragments shorter than     430 bp
  75% of sequence in fragments shorter than     485 bp
  80% of sequence in fragments shorter than     557 bp
  85% of sequence in fragments shorter than     650 bp
  90% of sequence in fragments shorter than     785 bp
  95% of sequence in fragments shorter than    1046 bp

# ScrFI.TspRI (CCNGG+CASTG) double digest, 20569 thousand fragments (11805099+8765240 sites), 144 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than      92 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     142 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     200 bp
  40% of sequence in fragments shorter than     231 bp
  45% of sequence in fragments shorter than     266 bp
  50% of sequence in fragments shorter than     303 bp
  55% of sequence in fragments shorter than     342 bp
  60% of sequence in fragments shorter than     387 bp
  65% of sequence in fragments shorter than     438 bp
  70% of sequence in fragments shorter than     498 bp
  75% of sequence in fragments shorter than     568 bp
  80% of sequence in fragments shorter than     656 bp
  85% of sequence in fragments shorter than     769 bp
  90% of sequence in fragments shorter than     939 bp
  95% of sequence in fragments shorter than    1233 bp

# MseI.PstI (TTAA+CTGCAG) double digest, 20535 thousand fragments (19214674+1321469 sites), 145 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      94 bp
  20% of sequence in fragments shorter than     117 bp
  25% of sequence in fragments shorter than     141 bp
  30% of sequence in fragments shorter than     164 bp
  35% of sequence in fragments shorter than     189 bp
  40% of sequence in fragments shorter than     217 bp
  45% of sequence in fragments shorter than     246 bp
  50% of sequence in fragments shorter than     278 bp
  55% of sequence in fragments shorter than     311 bp
  60% of sequence in fragments shorter than     345 bp
  65% of sequence in fragments shorter than     384 bp
  70% of sequence in fragments shorter than     432 bp
  75% of sequence in fragments shorter than     489 bp
  80% of sequence in fragments shorter than     562 bp
  85% of sequence in fragments shorter than     659 bp
  90% of sequence in fragments shorter than     800 bp
  95% of sequence in fragments shorter than    1093 bp

# BthCI.DdeI (GCNGC+CTNAG) double digest, 20521 thousand fragments (6209929+14312045 sites), 145 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      76 bp
  15% of sequence in fragments shorter than     101 bp
  20% of sequence in fragments shorter than     121 bp
  25% of sequence in fragments shorter than     142 bp
  30% of sequence in fragments shorter than     165 bp
  35% of sequence in fragments shorter than     185 bp
  40% of sequence in fragments shorter than     209 bp
  45% of sequence in fragments shorter than     236 bp
  50% of sequence in fragments shorter than     264 bp
  55% of sequence in fragments shorter than     294 bp
  60% of sequence in fragments shorter than     327 bp
  65% of sequence in fragments shorter than     362 bp
  70% of sequence in fragments shorter than     404 bp
  75% of sequence in fragments shorter than     453 bp
  80% of sequence in fragments shorter than     512 bp
  85% of sequence in fragments shorter than     586 bp
  90% of sequence in fragments shorter than     692 bp
  95% of sequence in fragments shorter than     882 bp

# BccI.DdeI (CCATC+CTNAG) double digest, 20482 thousand fragments (6170927+14312045 sites), 145 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     117 bp
  25% of sequence in fragments shorter than     136 bp
  30% of sequence in fragments shorter than     158 bp
  35% of sequence in fragments shorter than     175 bp
  40% of sequence in fragments shorter than     198 bp
  45% of sequence in fragments shorter than     222 bp
  50% of sequence in fragments shorter than     247 bp
  55% of sequence in fragments shorter than     274 bp
  60% of sequence in fragments shorter than     303 bp
  65% of sequence in fragments shorter than     335 bp
  70% of sequence in fragments shorter than     371 bp
  75% of sequence in fragments shorter than     415 bp
  80% of sequence in fragments shorter than     468 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     630 bp
  95% of sequence in fragments shorter than     794 bp

# BsmAI.CviAII (GTCTC+CATG) double digest, 20446 thousand fragments (6631585+13815697 sites), 145 bp average fragment length
   5% of sequence in fragments shorter than      48 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     121 bp
  25% of sequence in fragments shorter than     140 bp
  30% of sequence in fragments shorter than     162 bp
  35% of sequence in fragments shorter than     183 bp
  40% of sequence in fragments shorter than     207 bp
  45% of sequence in fragments shorter than     232 bp
  50% of sequence in fragments shorter than     258 bp
  55% of sequence in fragments shorter than     286 bp
  60% of sequence in fragments shorter than     315 bp
  65% of sequence in fragments shorter than     350 bp
  70% of sequence in fragments shorter than     390 bp
  75% of sequence in fragments shorter than     437 bp
  80% of sequence in fragments shorter than     493 bp
  85% of sequence in fragments shorter than     565 bp
  90% of sequence in fragments shorter than     669 bp
  95% of sequence in fragments shorter than     863 bp

# BscGI.MseI (CCCGT+TTAA) double digest, 20445 thousand fragments (1231070+19214674 sites), 145 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     142 bp
  30% of sequence in fragments shorter than     165 bp
  35% of sequence in fragments shorter than     190 bp
  40% of sequence in fragments shorter than     217 bp
  45% of sequence in fragments shorter than     246 bp
  50% of sequence in fragments shorter than     276 bp
  55% of sequence in fragments shorter than     308 bp
  60% of sequence in fragments shorter than     343 bp
  65% of sequence in fragments shorter than     381 bp
  70% of sequence in fragments shorter than     428 bp
  75% of sequence in fragments shorter than     484 bp
  80% of sequence in fragments shorter than     553 bp
  85% of sequence in fragments shorter than     645 bp
  90% of sequence in fragments shorter than     776 bp
  95% of sequence in fragments shorter than    1032 bp

# HaeIII.ScrFI (GGCC+CCNGG) double digest, 20366 thousand fragments (8562230+11805099 sites), 146 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      69 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     128 bp
  25% of sequence in fragments shorter than     162 bp
  30% of sequence in fragments shorter than     195 bp
  35% of sequence in fragments shorter than     234 bp
  40% of sequence in fragments shorter than     277 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     366 bp
  55% of sequence in fragments shorter than     422 bp
  60% of sequence in fragments shorter than     484 bp
  65% of sequence in fragments shorter than     553 bp
  70% of sequence in fragments shorter than     632 bp
  75% of sequence in fragments shorter than     725 bp
  80% of sequence in fragments shorter than     845 bp
  85% of sequence in fragments shorter than     991 bp
  90% of sequence in fragments shorter than    1204 bp
  95% of sequence in fragments shorter than    1573 bp

# DdeI.HphI (CTNAG+GGTGA) double digest, 20318 thousand fragments (14312045+6007336 sites), 146 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      76 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     118 bp
  25% of sequence in fragments shorter than     138 bp
  30% of sequence in fragments shorter than     160 bp
  35% of sequence in fragments shorter than     178 bp
  40% of sequence in fragments shorter than     202 bp
  45% of sequence in fragments shorter than     227 bp
  50% of sequence in fragments shorter than     253 bp
  55% of sequence in fragments shorter than     281 bp
  60% of sequence in fragments shorter than     311 bp
  65% of sequence in fragments shorter than     345 bp
  70% of sequence in fragments shorter than     383 bp
  75% of sequence in fragments shorter than     428 bp
  80% of sequence in fragments shorter than     484 bp
  85% of sequence in fragments shorter than     553 bp
  90% of sequence in fragments shorter than     654 bp
  95% of sequence in fragments shorter than     816 bp

# AccI.MseI (GTMKAC+TTAA) double digest, 20315 thousand fragments (1101063+19214674 sites), 146 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      95 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     143 bp
  30% of sequence in fragments shorter than     167 bp
  35% of sequence in fragments shorter than     194 bp
  40% of sequence in fragments shorter than     224 bp
  45% of sequence in fragments shorter than     256 bp
  50% of sequence in fragments shorter than     291 bp
  55% of sequence in fragments shorter than     326 bp
  60% of sequence in fragments shorter than     363 bp
  65% of sequence in fragments shorter than     409 bp
  70% of sequence in fragments shorter than     462 bp
  75% of sequence in fragments shorter than     530 bp
  80% of sequence in fragments shorter than     617 bp
  85% of sequence in fragments shorter than     732 bp
  90% of sequence in fragments shorter than     925 bp
  95% of sequence in fragments shorter than    1327 bp

# AluI.BspNCI (AGCT+CCAGA) double digest, 20309 thousand fragments (13027778+7282436 sites), 146 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     140 bp
  30% of sequence in fragments shorter than     162 bp
  35% of sequence in fragments shorter than     184 bp
  40% of sequence in fragments shorter than     206 bp
  45% of sequence in fragments shorter than     228 bp
  50% of sequence in fragments shorter than     253 bp
  55% of sequence in fragments shorter than     280 bp
  60% of sequence in fragments shorter than     309 bp
  65% of sequence in fragments shorter than     340 bp
  70% of sequence in fragments shorter than     374 bp
  75% of sequence in fragments shorter than     418 bp
  80% of sequence in fragments shorter than     469 bp
  85% of sequence in fragments shorter than     537 bp
  90% of sequence in fragments shorter than     633 bp
  95% of sequence in fragments shorter than     797 bp

# MseI.PpuMI (TTAA+RGGWCCY) double digest, 20299 thousand fragments (19214674+1085138 sites), 146 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     143 bp
  30% of sequence in fragments shorter than     167 bp
  35% of sequence in fragments shorter than     193 bp
  40% of sequence in fragments shorter than     220 bp
  45% of sequence in fragments shorter than     251 bp
  50% of sequence in fragments shorter than     284 bp
  55% of sequence in fragments shorter than     318 bp
  60% of sequence in fragments shorter than     353 bp
  65% of sequence in fragments shorter than     394 bp
  70% of sequence in fragments shorter than     443 bp
  75% of sequence in fragments shorter than     501 bp
  80% of sequence in fragments shorter than     577 bp
  85% of sequence in fragments shorter than     676 bp
  90% of sequence in fragments shorter than     821 bp
  95% of sequence in fragments shorter than    1116 bp

# MseI.PvuII (TTAA+CAGCTG) double digest, 20298 thousand fragments (19214674+1084260 sites), 146 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     143 bp
  30% of sequence in fragments shorter than     167 bp
  35% of sequence in fragments shorter than     192 bp
  40% of sequence in fragments shorter than     221 bp
  45% of sequence in fragments shorter than     252 bp
  50% of sequence in fragments shorter than     285 bp
  55% of sequence in fragments shorter than     319 bp
  60% of sequence in fragments shorter than     353 bp
  65% of sequence in fragments shorter than     394 bp
  70% of sequence in fragments shorter than     443 bp
  75% of sequence in fragments shorter than     502 bp
  80% of sequence in fragments shorter than     579 bp
  85% of sequence in fragments shorter than     681 bp
  90% of sequence in fragments shorter than     833 bp
  95% of sequence in fragments shorter than    1149 bp

# AluI.BspKT6I (AGCT+GATC) double digest, 20226 thousand fragments (13027778+7199434 sites), 147 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     114 bp
  25% of sequence in fragments shorter than     137 bp
  30% of sequence in fragments shorter than     159 bp
  35% of sequence in fragments shorter than     180 bp
  40% of sequence in fragments shorter than     203 bp
  45% of sequence in fragments shorter than     226 bp
  50% of sequence in fragments shorter than     252 bp
  55% of sequence in fragments shorter than     278 bp
  60% of sequence in fragments shorter than     307 bp
  65% of sequence in fragments shorter than     339 bp
  70% of sequence in fragments shorter than     374 bp
  75% of sequence in fragments shorter than     418 bp
  80% of sequence in fragments shorter than     467 bp
  85% of sequence in fragments shorter than     534 bp
  90% of sequence in fragments shorter than     630 bp
  95% of sequence in fragments shorter than     792 bp

# AvaI.MseI (CYCGRG+TTAA) double digest, 20219 thousand fragments (1005714+19214674 sites), 147 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     121 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     168 bp
  35% of sequence in fragments shorter than     193 bp
  40% of sequence in fragments shorter than     219 bp
  45% of sequence in fragments shorter than     247 bp
  50% of sequence in fragments shorter than     278 bp
  55% of sequence in fragments shorter than     311 bp
  60% of sequence in fragments shorter than     345 bp
  65% of sequence in fragments shorter than     385 bp
  70% of sequence in fragments shorter than     433 bp
  75% of sequence in fragments shorter than     491 bp
  80% of sequence in fragments shorter than     566 bp
  85% of sequence in fragments shorter than     664 bp
  90% of sequence in fragments shorter than     809 bp
  95% of sequence in fragments shorter than    1110 bp

# ApoI.CviAII (RAATTY+CATG) double digest, 20197 thousand fragments (6382433+13815697 sites), 147 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      76 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     139 bp
  30% of sequence in fragments shorter than     159 bp
  35% of sequence in fragments shorter than     180 bp
  40% of sequence in fragments shorter than     203 bp
  45% of sequence in fragments shorter than     226 bp
  50% of sequence in fragments shorter than     249 bp
  55% of sequence in fragments shorter than     275 bp
  60% of sequence in fragments shorter than     302 bp
  65% of sequence in fragments shorter than     332 bp
  70% of sequence in fragments shorter than     367 bp
  75% of sequence in fragments shorter than     408 bp
  80% of sequence in fragments shorter than     460 bp
  85% of sequence in fragments shorter than     526 bp
  90% of sequence in fragments shorter than     621 bp
  95% of sequence in fragments shorter than     793 bp

# BspHI.MseI (TCATGA+TTAA) double digest, 20192 thousand fragments (978290+19214674 sites), 147 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      95 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     143 bp
  30% of sequence in fragments shorter than     168 bp
  35% of sequence in fragments shorter than     195 bp
  40% of sequence in fragments shorter than     224 bp
  45% of sequence in fragments shorter than     257 bp
  50% of sequence in fragments shorter than     290 bp
  55% of sequence in fragments shorter than     326 bp
  60% of sequence in fragments shorter than     363 bp
  65% of sequence in fragments shorter than     410 bp
  70% of sequence in fragments shorter than     464 bp
  75% of sequence in fragments shorter than     532 bp
  80% of sequence in fragments shorter than     621 bp
  85% of sequence in fragments shorter than     739 bp
  90% of sequence in fragments shorter than     942 bp
  95% of sequence in fragments shorter than    1385 bp

# AvaIII.MseI (ATGCAT+TTAA) double digest, 20147 thousand fragments (933357+19214674 sites), 147 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     120 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     169 bp
  35% of sequence in fragments shorter than     197 bp
  40% of sequence in fragments shorter than     227 bp
  45% of sequence in fragments shorter than     261 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     369 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     471 bp
  75% of sequence in fragments shorter than     541 bp
  80% of sequence in fragments shorter than     631 bp
  85% of sequence in fragments shorter than     753 bp
  90% of sequence in fragments shorter than     960 bp
  95% of sequence in fragments shorter than    1407 bp

# MseI.StuI (TTAA+AGGCCT) double digest, 20139 thousand fragments (19214674+925729 sites), 147 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     120 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     169 bp
  35% of sequence in fragments shorter than     195 bp
  40% of sequence in fragments shorter than     224 bp
  45% of sequence in fragments shorter than     256 bp
  50% of sequence in fragments shorter than     288 bp
  55% of sequence in fragments shorter than     324 bp
  60% of sequence in fragments shorter than     359 bp
  65% of sequence in fragments shorter than     402 bp
  70% of sequence in fragments shorter than     451 bp
  75% of sequence in fragments shorter than     513 bp
  80% of sequence in fragments shorter than     590 bp
  85% of sequence in fragments shorter than     689 bp
  90% of sequence in fragments shorter than     841 bp
  95% of sequence in fragments shorter than    1142 bp

# MseI.NdeI (TTAA+CATATG) double digest, 20117 thousand fragments (19214674+903360 sites), 148 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than      95 bp
  20% of sequence in fragments shorter than     120 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     169 bp
  35% of sequence in fragments shorter than     197 bp
  40% of sequence in fragments shorter than     227 bp
  45% of sequence in fragments shorter than     260 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     369 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     471 bp
  75% of sequence in fragments shorter than     542 bp
  80% of sequence in fragments shorter than     633 bp
  85% of sequence in fragments shorter than     757 bp
  90% of sequence in fragments shorter than     970 bp
  95% of sequence in fragments shorter than    1439 bp

# AluI.Hin4II (AGCT+CCTTC) double digest, 20087 thousand fragments (13027778+7059915 sites), 148 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     167 bp
  35% of sequence in fragments shorter than     188 bp
  40% of sequence in fragments shorter than     210 bp
  45% of sequence in fragments shorter than     233 bp
  50% of sequence in fragments shorter than     258 bp
  55% of sequence in fragments shorter than     284 bp
  60% of sequence in fragments shorter than     313 bp
  65% of sequence in fragments shorter than     345 bp
  70% of sequence in fragments shorter than     380 bp
  75% of sequence in fragments shorter than     424 bp
  80% of sequence in fragments shorter than     476 bp
  85% of sequence in fragments shorter than     543 bp
  90% of sequence in fragments shorter than     641 bp
  95% of sequence in fragments shorter than     799 bp

# HindIII.MseI (AAGCTT+TTAA) double digest, 20074 thousand fragments (860365+19214674 sites), 148 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     121 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     229 bp
  45% of sequence in fragments shorter than     262 bp
  50% of sequence in fragments shorter than     297 bp
  55% of sequence in fragments shorter than     332 bp
  60% of sequence in fragments shorter than     370 bp
  65% of sequence in fragments shorter than     417 bp
  70% of sequence in fragments shorter than     471 bp
  75% of sequence in fragments shorter than     538 bp
  80% of sequence in fragments shorter than     626 bp
  85% of sequence in fragments shorter than     742 bp
  90% of sequence in fragments shorter than     943 bp
  95% of sequence in fragments shorter than    1388 bp

# MseI.XbaI (TTAA+TCTAGA) double digest, 20065 thousand fragments (19214674+850998 sites), 148 bp average fragment length
   5% of sequence in fragments shorter than      45 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     120 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     169 bp
  35% of sequence in fragments shorter than     196 bp
  40% of sequence in fragments shorter than     226 bp
  45% of sequence in fragments shorter than     259 bp
  50% of sequence in fragments shorter than     295 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     414 bp
  70% of sequence in fragments shorter than     467 bp
  75% of sequence in fragments shorter than     536 bp
  80% of sequence in fragments shorter than     624 bp
  85% of sequence in fragments shorter than     738 bp
  90% of sequence in fragments shorter than     922 bp
  95% of sequence in fragments shorter than    1317 bp

# EcoRI.MseI (GAATTC+TTAA) double digest, 20061 thousand fragments (847401+19214674 sites), 148 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     120 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     169 bp
  35% of sequence in fragments shorter than     196 bp
  40% of sequence in fragments shorter than     225 bp
  45% of sequence in fragments shorter than     258 bp
  50% of sequence in fragments shorter than     293 bp
  55% of sequence in fragments shorter than     329 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     414 bp
  70% of sequence in fragments shorter than     468 bp
  75% of sequence in fragments shorter than     536 bp
  80% of sequence in fragments shorter than     625 bp
  85% of sequence in fragments shorter than     738 bp
  90% of sequence in fragments shorter than     939 bp
  95% of sequence in fragments shorter than    1379 bp

# BsrI.DdeI (ACTGG+CTNAG) double digest, 20052 thousand fragments (5741310+14312045 sites), 148 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      77 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     120 bp
  25% of sequence in fragments shorter than     139 bp
  30% of sequence in fragments shorter than     161 bp
  35% of sequence in fragments shorter than     178 bp
  40% of sequence in fragments shorter than     200 bp
  45% of sequence in fragments shorter than     224 bp
  50% of sequence in fragments shorter than     249 bp
  55% of sequence in fragments shorter than     276 bp
  60% of sequence in fragments shorter than     306 bp
  65% of sequence in fragments shorter than     338 bp
  70% of sequence in fragments shorter than     373 bp
  75% of sequence in fragments shorter than     416 bp
  80% of sequence in fragments shorter than     466 bp
  85% of sequence in fragments shorter than     531 bp
  90% of sequence in fragments shorter than     624 bp
  95% of sequence in fragments shorter than     780 bp

# BthCI.CviAII (GCNGC+CATG) double digest, 20025 thousand fragments (6209929+13815697 sites), 148 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      78 bp
  15% of sequence in fragments shorter than     101 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     172 bp
  35% of sequence in fragments shorter than     197 bp
  40% of sequence in fragments shorter than     222 bp
  45% of sequence in fragments shorter than     247 bp
  50% of sequence in fragments shorter than     275 bp
  55% of sequence in fragments shorter than     303 bp
  60% of sequence in fragments shorter than     335 bp
  65% of sequence in fragments shorter than     370 bp
  70% of sequence in fragments shorter than     412 bp
  75% of sequence in fragments shorter than     460 bp
  80% of sequence in fragments shorter than     519 bp
  85% of sequence in fragments shorter than     595 bp
  90% of sequence in fragments shorter than     701 bp
  95% of sequence in fragments shorter than     880 bp

# BglII.MseI (AGATCT+TTAA) double digest, 19988 thousand fragments (774732+19214674 sites), 149 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     121 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     170 bp
  35% of sequence in fragments shorter than     197 bp
  40% of sequence in fragments shorter than     228 bp
  45% of sequence in fragments shorter than     261 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     369 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     470 bp
  75% of sequence in fragments shorter than     540 bp
  80% of sequence in fragments shorter than     630 bp
  85% of sequence in fragments shorter than     751 bp
  90% of sequence in fragments shorter than     956 bp
  95% of sequence in fragments shorter than    1405 bp

# BccI.CviAII (CCATC+CATG) double digest, 19986 thousand fragments (6170927+13815697 sites), 149 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      77 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     166 bp
  35% of sequence in fragments shorter than     188 bp
  40% of sequence in fragments shorter than     212 bp
  45% of sequence in fragments shorter than     236 bp
  50% of sequence in fragments shorter than     261 bp
  55% of sequence in fragments shorter than     289 bp
  60% of sequence in fragments shorter than     320 bp
  65% of sequence in fragments shorter than     353 bp
  70% of sequence in fragments shorter than     392 bp
  75% of sequence in fragments shorter than     438 bp
  80% of sequence in fragments shorter than     492 bp
  85% of sequence in fragments shorter than     563 bp
  90% of sequence in fragments shorter than     664 bp
  95% of sequence in fragments shorter than     849 bp

# MseI.NcoI (TTAA+CCATGG) double digest, 19973 thousand fragments (19214674+759594 sites), 149 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     121 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     170 bp
  35% of sequence in fragments shorter than     197 bp
  40% of sequence in fragments shorter than     227 bp
  45% of sequence in fragments shorter than     259 bp
  50% of sequence in fragments shorter than     293 bp
  55% of sequence in fragments shorter than     328 bp
  60% of sequence in fragments shorter than     363 bp
  65% of sequence in fragments shorter than     408 bp
  70% of sequence in fragments shorter than     459 bp
  75% of sequence in fragments shorter than     524 bp
  80% of sequence in fragments shorter than     607 bp
  85% of sequence in fragments shorter than     717 bp
  90% of sequence in fragments shorter than     894 bp
  95% of sequence in fragments shorter than    1262 bp

# BclI.MseI (TGATCA+TTAA) double digest, 19952 thousand fragments (738785+19214674 sites), 149 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      96 bp
  20% of sequence in fragments shorter than     120 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     170 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     229 bp
  45% of sequence in fragments shorter than     262 bp
  50% of sequence in fragments shorter than     297 bp
  55% of sequence in fragments shorter than     332 bp
  60% of sequence in fragments shorter than     370 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     471 bp
  75% of sequence in fragments shorter than     540 bp
  80% of sequence in fragments shorter than     628 bp
  85% of sequence in fragments shorter than     746 bp
  90% of sequence in fragments shorter than     948 bp
  95% of sequence in fragments shorter than    1405 bp

# FnuDII.MseI (CGCG+TTAA) double digest, 19948 thousand fragments (733940+19214674 sites), 149 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     228 bp
  45% of sequence in fragments shorter than     259 bp
  50% of sequence in fragments shorter than     292 bp
  55% of sequence in fragments shorter than     327 bp
  60% of sequence in fragments shorter than     363 bp
  65% of sequence in fragments shorter than     407 bp
  70% of sequence in fragments shorter than     458 bp
  75% of sequence in fragments shorter than     523 bp
  80% of sequence in fragments shorter than     605 bp
  85% of sequence in fragments shorter than     711 bp
  90% of sequence in fragments shorter than     883 bp
  95% of sequence in fragments shorter than    1233 bp

# BsaAI.MseI (YACGTR+TTAA) double digest, 19927 thousand fragments (713801+19214674 sites), 149 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     121 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     170 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     227 bp
  45% of sequence in fragments shorter than     260 bp
  50% of sequence in fragments shorter than     295 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     369 bp
  65% of sequence in fragments shorter than     414 bp
  70% of sequence in fragments shorter than     468 bp
  75% of sequence in fragments shorter than     535 bp
  80% of sequence in fragments shorter than     623 bp
  85% of sequence in fragments shorter than     737 bp
  90% of sequence in fragments shorter than     928 bp
  95% of sequence in fragments shorter than    1322 bp

# BceAI.MseI (ACGGC+TTAA) double digest, 19919 thousand fragments (705178+19214674 sites), 149 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     228 bp
  45% of sequence in fragments shorter than     260 bp
  50% of sequence in fragments shorter than     293 bp
  55% of sequence in fragments shorter than     328 bp
  60% of sequence in fragments shorter than     364 bp
  65% of sequence in fragments shorter than     408 bp
  70% of sequence in fragments shorter than     459 bp
  75% of sequence in fragments shorter than     522 bp
  80% of sequence in fragments shorter than     602 bp
  85% of sequence in fragments shorter than     705 bp
  90% of sequence in fragments shorter than     867 bp
  95% of sequence in fragments shorter than    1197 bp

# BciVI.MseI (GTATCC+TTAA) double digest, 19910 thousand fragments (696093+19214674 sites), 149 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      72 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     121 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     229 bp
  45% of sequence in fragments shorter than     262 bp
  50% of sequence in fragments shorter than     297 bp
  55% of sequence in fragments shorter than     333 bp
  60% of sequence in fragments shorter than     370 bp
  65% of sequence in fragments shorter than     418 bp
  70% of sequence in fragments shorter than     473 bp
  75% of sequence in fragments shorter than     543 bp
  80% of sequence in fragments shorter than     632 bp
  85% of sequence in fragments shorter than     752 bp
  90% of sequence in fragments shorter than     955 bp
  95% of sequence in fragments shorter than    1395 bp

# AflII.MseI (CTTAAG+TTAA) double digest, 19857 thousand fragments (643284+19214674 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     310 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     387 bp
  65% of sequence in fragments shorter than     437 bp
  70% of sequence in fragments shorter than     495 bp
  75% of sequence in fragments shorter than     571 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     794 bp
  90% of sequence in fragments shorter than    1022 bp
  95% of sequence in fragments shorter than    1518 bp

# HaeII.MseI (RGCGCY+TTAA) double digest, 19844 thousand fragments (630101+19214674 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     227 bp
  45% of sequence in fragments shorter than     258 bp
  50% of sequence in fragments shorter than     291 bp
  55% of sequence in fragments shorter than     326 bp
  60% of sequence in fragments shorter than     361 bp
  65% of sequence in fragments shorter than     404 bp
  70% of sequence in fragments shorter than     455 bp
  75% of sequence in fragments shorter than     517 bp
  80% of sequence in fragments shorter than     596 bp
  85% of sequence in fragments shorter than     694 bp
  90% of sequence in fragments shorter than     847 bp
  95% of sequence in fragments shorter than    1140 bp

# CviAII.HphI (CATG+GGTGA) double digest, 19822 thousand fragments (13815697+6007336 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      78 bp
  15% of sequence in fragments shorter than     101 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     166 bp
  35% of sequence in fragments shorter than     189 bp
  40% of sequence in fragments shorter than     214 bp
  45% of sequence in fragments shorter than     238 bp
  50% of sequence in fragments shorter than     264 bp
  55% of sequence in fragments shorter than     291 bp
  60% of sequence in fragments shorter than     321 bp
  65% of sequence in fragments shorter than     354 bp
  70% of sequence in fragments shorter than     392 bp
  75% of sequence in fragments shorter than     438 bp
  80% of sequence in fragments shorter than     491 bp
  85% of sequence in fragments shorter than     561 bp
  90% of sequence in fragments shorter than     658 bp
  95% of sequence in fragments shorter than     827 bp

# MseI.SacI (TTAA+GAGCTC) double digest, 19817 thousand fragments (19214674+603323 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     229 bp
  45% of sequence in fragments shorter than     261 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     413 bp
  70% of sequence in fragments shorter than     466 bp
  75% of sequence in fragments shorter than     532 bp
  80% of sequence in fragments shorter than     618 bp
  85% of sequence in fragments shorter than     727 bp
  90% of sequence in fragments shorter than     912 bp
  95% of sequence in fragments shorter than    1293 bp

# AvrII.MseI (CCTAGG+TTAA) double digest, 19809 thousand fragments (594956+19214674 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     199 bp
  40% of sequence in fragments shorter than     229 bp
  45% of sequence in fragments shorter than     262 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     369 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     470 bp
  75% of sequence in fragments shorter than     538 bp
  80% of sequence in fragments shorter than     625 bp
  85% of sequence in fragments shorter than     741 bp
  90% of sequence in fragments shorter than     936 bp
  95% of sequence in fragments shorter than    1356 bp

# HgaI.MseI (GACGC+TTAA) double digest, 19786 thousand fragments (571889+19214674 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     172 bp
  35% of sequence in fragments shorter than     199 bp
  40% of sequence in fragments shorter than     229 bp
  45% of sequence in fragments shorter than     262 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     330 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     412 bp
  70% of sequence in fragments shorter than     464 bp
  75% of sequence in fragments shorter than     528 bp
  80% of sequence in fragments shorter than     610 bp
  85% of sequence in fragments shorter than     714 bp
  90% of sequence in fragments shorter than     882 bp
  95% of sequence in fragments shorter than    1212 bp

# EciI.MseI (GGCGGA+TTAA) double digest, 19785 thousand fragments (571273+19214674 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     170 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     227 bp
  45% of sequence in fragments shorter than     257 bp
  50% of sequence in fragments shorter than     289 bp
  55% of sequence in fragments shorter than     324 bp
  60% of sequence in fragments shorter than     360 bp
  65% of sequence in fragments shorter than     404 bp
  70% of sequence in fragments shorter than     456 bp
  75% of sequence in fragments shorter than     522 bp
  80% of sequence in fragments shorter than     605 bp
  85% of sequence in fragments shorter than     715 bp
  90% of sequence in fragments shorter than     896 bp
  95% of sequence in fragments shorter than    1280 bp

# MfeI.MseI (CAATTG+TTAA) double digest, 19778 thousand fragments (564303+19214674 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     172 bp
  35% of sequence in fragments shorter than     200 bp
  40% of sequence in fragments shorter than     231 bp
  45% of sequence in fragments shorter than     264 bp
  50% of sequence in fragments shorter than     299 bp
  55% of sequence in fragments shorter than     336 bp
  60% of sequence in fragments shorter than     375 bp
  65% of sequence in fragments shorter than     424 bp
  70% of sequence in fragments shorter than     480 bp
  75% of sequence in fragments shorter than     552 bp
  80% of sequence in fragments shorter than     644 bp
  85% of sequence in fragments shorter than     768 bp
  90% of sequence in fragments shorter than     981 bp
  95% of sequence in fragments shorter than    1445 bp

# MseI.SphI (TTAA+GCATGC) double digest, 19765 thousand fragments (19214674+550952 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     172 bp
  35% of sequence in fragments shorter than     199 bp
  40% of sequence in fragments shorter than     229 bp
  45% of sequence in fragments shorter than     261 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     415 bp
  70% of sequence in fragments shorter than     469 bp
  75% of sequence in fragments shorter than     537 bp
  80% of sequence in fragments shorter than     625 bp
  85% of sequence in fragments shorter than     740 bp
  90% of sequence in fragments shorter than     936 bp
  95% of sequence in fragments shorter than    1358 bp

# MseI.ScaI (TTAA+AGTACT) double digest, 19757 thousand fragments (19214674+543793 sites), 150 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      97 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     173 bp
  35% of sequence in fragments shorter than     201 bp
  40% of sequence in fragments shorter than     232 bp
  45% of sequence in fragments shorter than     266 bp
  50% of sequence in fragments shorter than     300 bp
  55% of sequence in fragments shorter than     337 bp
  60% of sequence in fragments shorter than     376 bp
  65% of sequence in fragments shorter than     424 bp
  70% of sequence in fragments shorter than     480 bp
  75% of sequence in fragments shorter than     552 bp
  80% of sequence in fragments shorter than     644 bp
  85% of sequence in fragments shorter than     770 bp
  90% of sequence in fragments shorter than     983 bp
  95% of sequence in fragments shorter than    1454 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# ApaLI.MseI (GTGCAC+TTAA) double digest, 19710 thousand fragments (496255+19214674 sites), 151 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     173 bp
  35% of sequence in fragments shorter than     200 bp
  40% of sequence in fragments shorter than     231 bp
  45% of sequence in fragments shorter than     264 bp
  50% of sequence in fragments shorter than     298 bp
  55% of sequence in fragments shorter than     333 bp
  60% of sequence in fragments shorter than     371 bp
  65% of sequence in fragments shorter than     418 bp
  70% of sequence in fragments shorter than     471 bp
  75% of sequence in fragments shorter than     540 bp
  80% of sequence in fragments shorter than     627 bp
  85% of sequence in fragments shorter than     743 bp
  90% of sequence in fragments shorter than     938 bp
  95% of sequence in fragments shorter than    1345 bp

# ApaI.MseI (GGGCCC+TTAA) double digest, 19674 thousand fragments (460339+19214674 sites), 151 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     173 bp
  35% of sequence in fragments shorter than     201 bp
  40% of sequence in fragments shorter than     232 bp
  45% of sequence in fragments shorter than     265 bp
  50% of sequence in fragments shorter than     298 bp
  55% of sequence in fragments shorter than     334 bp
  60% of sequence in fragments shorter than     370 bp
  65% of sequence in fragments shorter than     415 bp
  70% of sequence in fragments shorter than     467 bp
  75% of sequence in fragments shorter than     532 bp
  80% of sequence in fragments shorter than     613 bp
  85% of sequence in fragments shorter than     717 bp
  90% of sequence in fragments shorter than     883 bp
  95% of sequence in fragments shorter than    1211 bp

# EcoRV.MseI (GATATC+TTAA) double digest, 19660 thousand fragments (446473+19214674 sites), 151 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     173 bp
  35% of sequence in fragments shorter than     201 bp
  40% of sequence in fragments shorter than     233 bp
  45% of sequence in fragments shorter than     266 bp
  50% of sequence in fragments shorter than     301 bp
  55% of sequence in fragments shorter than     338 bp
  60% of sequence in fragments shorter than     377 bp
  65% of sequence in fragments shorter than     425 bp
  70% of sequence in fragments shorter than     482 bp
  75% of sequence in fragments shorter than     555 bp
  80% of sequence in fragments shorter than     647 bp
  85% of sequence in fragments shorter than     773 bp
  90% of sequence in fragments shorter than     981 bp
  95% of sequence in fragments shorter than    1450 bp

# AluI.BsmAI (AGCT+GTCTC) double digest, 19658 thousand fragments (13027778+6631585 sites), 151 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     118 bp
  25% of sequence in fragments shorter than     142 bp
  30% of sequence in fragments shorter than     167 bp
  35% of sequence in fragments shorter than     191 bp
  40% of sequence in fragments shorter than     216 bp
  45% of sequence in fragments shorter than     241 bp
  50% of sequence in fragments shorter than     269 bp
  55% of sequence in fragments shorter than     299 bp
  60% of sequence in fragments shorter than     331 bp
  65% of sequence in fragments shorter than     366 bp
  70% of sequence in fragments shorter than     408 bp
  75% of sequence in fragments shorter than     457 bp
  80% of sequence in fragments shorter than     516 bp
  85% of sequence in fragments shorter than     593 bp
  90% of sequence in fragments shorter than     702 bp
  95% of sequence in fragments shorter than     900 bp

# MseI.SpeI (TTAA+ACTAGT) double digest, 19614 thousand fragments (19214674+400286 sites), 151 bp average fragment length
   5% of sequence in fragments shorter than      46 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     203 bp
  40% of sequence in fragments shorter than     233 bp
  45% of sequence in fragments shorter than     267 bp
  50% of sequence in fragments shorter than     303 bp
  55% of sequence in fragments shorter than     341 bp
  60% of sequence in fragments shorter than     380 bp
  65% of sequence in fragments shorter than     428 bp
  70% of sequence in fragments shorter than     485 bp
  75% of sequence in fragments shorter than     558 bp
  80% of sequence in fragments shorter than     651 bp
  85% of sequence in fragments shorter than     779 bp
  90% of sequence in fragments shorter than     999 bp
  95% of sequence in fragments shorter than    1476 bp

# HpaI.MseI (GTTAAC+TTAA) double digest, 19603 thousand fragments (389418+19214674 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     310 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     387 bp
  65% of sequence in fragments shorter than     437 bp
  70% of sequence in fragments shorter than     495 bp
  75% of sequence in fragments shorter than     571 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     794 bp
  90% of sequence in fragments shorter than    1022 bp
  95% of sequence in fragments shorter than    1518 bp

# BbvI.DdeI (GCAGC+CTNAG) double digest, 19601 thousand fragments (5290051+14312045 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      80 bp
  15% of sequence in fragments shorter than     105 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     190 bp
  40% of sequence in fragments shorter than     215 bp
  45% of sequence in fragments shorter than     242 bp
  50% of sequence in fragments shorter than     270 bp
  55% of sequence in fragments shorter than     301 bp
  60% of sequence in fragments shorter than     334 bp
  65% of sequence in fragments shorter than     369 bp
  70% of sequence in fragments shorter than     411 bp
  75% of sequence in fragments shorter than     461 bp
  80% of sequence in fragments shorter than     520 bp
  85% of sequence in fragments shorter than     594 bp
  90% of sequence in fragments shorter than     702 bp
  95% of sequence in fragments shorter than     892 bp

# MseI.SapI (TTAA+GCTCTTC) double digest, 19591 thousand fragments (19214674+377161 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     202 bp
  40% of sequence in fragments shorter than     233 bp
  45% of sequence in fragments shorter than     266 bp
  50% of sequence in fragments shorter than     301 bp
  55% of sequence in fragments shorter than     338 bp
  60% of sequence in fragments shorter than     376 bp
  65% of sequence in fragments shorter than     423 bp
  70% of sequence in fragments shorter than     479 bp
  75% of sequence in fragments shorter than     549 bp
  80% of sequence in fragments shorter than     639 bp
  85% of sequence in fragments shorter than     759 bp
  90% of sequence in fragments shorter than     961 bp
  95% of sequence in fragments shorter than    1393 bp

# MseI.SmaI (TTAA+CCCGGG) double digest, 19591 thousand fragments (19214674+376940 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     202 bp
  40% of sequence in fragments shorter than     230 bp
  45% of sequence in fragments shorter than     262 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     413 bp
  70% of sequence in fragments shorter than     467 bp
  75% of sequence in fragments shorter than     533 bp
  80% of sequence in fragments shorter than     618 bp
  85% of sequence in fragments shorter than     727 bp
  90% of sequence in fragments shorter than     908 bp
  95% of sequence in fragments shorter than    1277 bp

# BamHI.MseI (GGATCC+TTAA) double digest, 19580 thousand fragments (365999+19214674 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     202 bp
  40% of sequence in fragments shorter than     234 bp
  45% of sequence in fragments shorter than     266 bp
  50% of sequence in fragments shorter than     302 bp
  55% of sequence in fragments shorter than     338 bp
  60% of sequence in fragments shorter than     376 bp
  65% of sequence in fragments shorter than     423 bp
  70% of sequence in fragments shorter than     478 bp
  75% of sequence in fragments shorter than     548 bp
  80% of sequence in fragments shorter than     636 bp
  85% of sequence in fragments shorter than     754 bp
  90% of sequence in fragments shorter than     951 bp
  95% of sequence in fragments shorter than    1372 bp

# BsrI.CviAII (ACTGG+CATG) double digest, 19556 thousand fragments (5741310+13815697 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      77 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     123 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     166 bp
  35% of sequence in fragments shorter than     188 bp
  40% of sequence in fragments shorter than     212 bp
  45% of sequence in fragments shorter than     236 bp
  50% of sequence in fragments shorter than     262 bp
  55% of sequence in fragments shorter than     290 bp
  60% of sequence in fragments shorter than     319 bp
  65% of sequence in fragments shorter than     353 bp
  70% of sequence in fragments shorter than     392 bp
  75% of sequence in fragments shorter than     438 bp
  80% of sequence in fragments shorter than     494 bp
  85% of sequence in fragments shorter than     566 bp
  90% of sequence in fragments shorter than     667 bp
  95% of sequence in fragments shorter than     847 bp

# BstEII.MseI (GGTNACC+TTAA) double digest, 19550 thousand fragments (335865+19214674 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      98 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     203 bp
  40% of sequence in fragments shorter than     234 bp
  45% of sequence in fragments shorter than     267 bp
  50% of sequence in fragments shorter than     302 bp
  55% of sequence in fragments shorter than     339 bp
  60% of sequence in fragments shorter than     377 bp
  65% of sequence in fragments shorter than     425 bp
  70% of sequence in fragments shorter than     480 bp
  75% of sequence in fragments shorter than     550 bp
  80% of sequence in fragments shorter than     639 bp
  85% of sequence in fragments shorter than     759 bp
  90% of sequence in fragments shorter than     960 bp
  95% of sequence in fragments shorter than    1385 bp

# DdeI.FokI (CTNAG+GGATG) double digest, 19512 thousand fragments (14312045+5201116 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      81 bp
  15% of sequence in fragments shorter than     104 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     144 bp
  30% of sequence in fragments shorter than     166 bp
  35% of sequence in fragments shorter than     184 bp
  40% of sequence in fragments shorter than     208 bp
  45% of sequence in fragments shorter than     232 bp
  50% of sequence in fragments shorter than     259 bp
  55% of sequence in fragments shorter than     287 bp
  60% of sequence in fragments shorter than     318 bp
  65% of sequence in fragments shorter than     350 bp
  70% of sequence in fragments shorter than     389 bp
  75% of sequence in fragments shorter than     434 bp
  80% of sequence in fragments shorter than     490 bp
  85% of sequence in fragments shorter than     559 bp
  90% of sequence in fragments shorter than     658 bp
  95% of sequence in fragments shorter than     822 bp

# MseI.SexAI (TTAA+ACCWGGT) double digest, 19512 thousand fragments (19214674+297900 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     175 bp
  35% of sequence in fragments shorter than     203 bp
  40% of sequence in fragments shorter than     234 bp
  45% of sequence in fragments shorter than     268 bp
  50% of sequence in fragments shorter than     303 bp
  55% of sequence in fragments shorter than     340 bp
  60% of sequence in fragments shorter than     378 bp
  65% of sequence in fragments shorter than     426 bp
  70% of sequence in fragments shorter than     482 bp
  75% of sequence in fragments shorter than     553 bp
  80% of sequence in fragments shorter than     643 bp
  85% of sequence in fragments shorter than     765 bp
  90% of sequence in fragments shorter than     971 bp
  95% of sequence in fragments shorter than    1412 bp

# KpnI.MseI (GGTACC+TTAA) double digest, 19502 thousand fragments (287974+19214674 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     202 bp
  40% of sequence in fragments shorter than     234 bp
  45% of sequence in fragments shorter than     268 bp
  50% of sequence in fragments shorter than     303 bp
  55% of sequence in fragments shorter than     340 bp
  60% of sequence in fragments shorter than     379 bp
  65% of sequence in fragments shorter than     427 bp
  70% of sequence in fragments shorter than     482 bp
  75% of sequence in fragments shorter than     553 bp
  80% of sequence in fragments shorter than     644 bp
  85% of sequence in fragments shorter than     763 bp
  90% of sequence in fragments shorter than     972 bp
  95% of sequence in fragments shorter than    1432 bp

# MseI.NheI (TTAA+GCTAGC) double digest, 19501 thousand fragments (19214674+287384 sites), 152 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     149 bp
  30% of sequence in fragments shorter than     175 bp
  35% of sequence in fragments shorter than     204 bp
  40% of sequence in fragments shorter than     235 bp
  45% of sequence in fragments shorter than     268 bp
  50% of sequence in fragments shorter than     304 bp
  55% of sequence in fragments shorter than     341 bp
  60% of sequence in fragments shorter than     380 bp
  65% of sequence in fragments shorter than     428 bp
  70% of sequence in fragments shorter than     485 bp
  75% of sequence in fragments shorter than     557 bp
  80% of sequence in fragments shorter than     649 bp
  85% of sequence in fragments shorter than     774 bp
  90% of sequence in fragments shorter than     987 bp
  95% of sequence in fragments shorter than    1444 bp

# BsrBI.MseI (CCGCTC+TTAA) double digest, 19449 thousand fragments (235523+19214674 sites), 153 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     149 bp
  30% of sequence in fragments shorter than     175 bp
  35% of sequence in fragments shorter than     204 bp
  40% of sequence in fragments shorter than     235 bp
  45% of sequence in fragments shorter than     269 bp
  50% of sequence in fragments shorter than     303 bp
  55% of sequence in fragments shorter than     340 bp
  60% of sequence in fragments shorter than     378 bp
  65% of sequence in fragments shorter than     426 bp
  70% of sequence in fragments shorter than     480 bp
  75% of sequence in fragments shorter than     550 bp
  80% of sequence in fragments shorter than     637 bp
  85% of sequence in fragments shorter than     752 bp
  90% of sequence in fragments shorter than     941 bp
  95% of sequence in fragments shorter than    1328 bp

# KasI.MseI (GGCGCC+TTAA) double digest, 19444 thousand fragments (230381+19214674 sites), 153 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     175 bp
  35% of sequence in fragments shorter than     203 bp
  40% of sequence in fragments shorter than     234 bp
  45% of sequence in fragments shorter than     266 bp
  50% of sequence in fragments shorter than     301 bp
  55% of sequence in fragments shorter than     337 bp
  60% of sequence in fragments shorter than     375 bp
  65% of sequence in fragments shorter than     422 bp
  70% of sequence in fragments shorter than     477 bp
  75% of sequence in fragments shorter than     547 bp
  80% of sequence in fragments shorter than     635 bp
  85% of sequence in fragments shorter than     752 bp
  90% of sequence in fragments shorter than     946 bp
  95% of sequence in fragments shorter than    1355 bp

# MseI.SwaI (TTAA+ATTTAAAT) double digest, 19440 thousand fragments (19214674+226602 sites), 153 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     310 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     387 bp
  65% of sequence in fragments shorter than     437 bp
  70% of sequence in fragments shorter than     495 bp
  75% of sequence in fragments shorter than     571 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     794 bp
  90% of sequence in fragments shorter than    1022 bp
  95% of sequence in fragments shorter than    1518 bp

# CviQI.DdeI (GTAC+CTNAG) double digest, 19426 thousand fragments (5115078+14312045 sites), 153 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      81 bp
  15% of sequence in fragments shorter than     105 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     168 bp
  35% of sequence in fragments shorter than     185 bp
  40% of sequence in fragments shorter than     208 bp
  45% of sequence in fragments shorter than     232 bp
  50% of sequence in fragments shorter than     257 bp
  55% of sequence in fragments shorter than     284 bp
  60% of sequence in fragments shorter than     312 bp
  65% of sequence in fragments shorter than     344 bp
  70% of sequence in fragments shorter than     380 bp
  75% of sequence in fragments shorter than     422 bp
  80% of sequence in fragments shorter than     473 bp
  85% of sequence in fragments shorter than     538 bp
  90% of sequence in fragments shorter than     631 bp
  95% of sequence in fragments shorter than     777 bp

# AluI.ApoI (AGCT+RAATTY) double digest, 19409 thousand fragments (13027778+6382433 sites), 153 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      78 bp
  15% of sequence in fragments shorter than     102 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     167 bp
  35% of sequence in fragments shorter than     188 bp
  40% of sequence in fragments shorter than     209 bp
  45% of sequence in fragments shorter than     231 bp
  50% of sequence in fragments shorter than     253 bp
  55% of sequence in fragments shorter than     278 bp
  60% of sequence in fragments shorter than     306 bp
  65% of sequence in fragments shorter than     335 bp
  70% of sequence in fragments shorter than     367 bp
  75% of sequence in fragments shorter than     407 bp
  80% of sequence in fragments shorter than     455 bp
  85% of sequence in fragments shorter than     516 bp
  90% of sequence in fragments shorter than     600 bp
  95% of sequence in fragments shorter than     752 bp

# AclI.MseI (AACGTT+TTAA) double digest, 19386 thousand fragments (172706+19214674 sites), 153 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     176 bp
  35% of sequence in fragments shorter than     205 bp
  40% of sequence in fragments shorter than     237 bp
  45% of sequence in fragments shorter than     272 bp
  50% of sequence in fragments shorter than     307 bp
  55% of sequence in fragments shorter than     344 bp
  60% of sequence in fragments shorter than     384 bp
  65% of sequence in fragments shorter than     433 bp
  70% of sequence in fragments shorter than     491 bp
  75% of sequence in fragments shorter than     566 bp
  80% of sequence in fragments shorter than     659 bp
  85% of sequence in fragments shorter than     786 bp
  90% of sequence in fragments shorter than    1010 bp
  95% of sequence in fragments shorter than    1494 bp

# MseI.PacI (TTAA+TTAATTAA) double digest, 19373 thousand fragments (19214674+159455 sites), 153 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     310 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     387 bp
  65% of sequence in fragments shorter than     437 bp
  70% of sequence in fragments shorter than     495 bp
  75% of sequence in fragments shorter than     571 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     794 bp
  90% of sequence in fragments shorter than    1022 bp
  95% of sequence in fragments shorter than    1518 bp

# Hpy99I.MseI (CGWCG+TTAA) double digest, 19372 thousand fragments (157917+19214674 sites), 153 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     176 bp
  35% of sequence in fragments shorter than     205 bp
  40% of sequence in fragments shorter than     237 bp
  45% of sequence in fragments shorter than     271 bp
  50% of sequence in fragments shorter than     305 bp
  55% of sequence in fragments shorter than     343 bp
  60% of sequence in fragments shorter than     382 bp
  65% of sequence in fragments shorter than     430 bp
  70% of sequence in fragments shorter than     485 bp
  75% of sequence in fragments shorter than     557 bp
  80% of sequence in fragments shorter than     646 bp
  85% of sequence in fragments shorter than     767 bp
  90% of sequence in fragments shorter than     964 bp
  95% of sequence in fragments shorter than    1384 bp

# MseI.SnaBI (TTAA+TACGTA) double digest, 19346 thousand fragments (19214674+132299 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     238 bp
  45% of sequence in fragments shorter than     272 bp
  50% of sequence in fragments shorter than     308 bp
  55% of sequence in fragments shorter than     344 bp
  60% of sequence in fragments shorter than     384 bp
  65% of sequence in fragments shorter than     434 bp
  70% of sequence in fragments shorter than     492 bp
  75% of sequence in fragments shorter than     567 bp
  80% of sequence in fragments shorter than     660 bp
  85% of sequence in fragments shorter than     788 bp
  90% of sequence in fragments shorter than    1013 bp
  95% of sequence in fragments shorter than    1483 bp

# MseI.NaeI (TTAA+GCCGGC) double digest, 19339 thousand fragments (19214674+125692 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     237 bp
  45% of sequence in fragments shorter than     271 bp
  50% of sequence in fragments shorter than     306 bp
  55% of sequence in fragments shorter than     343 bp
  60% of sequence in fragments shorter than     382 bp
  65% of sequence in fragments shorter than     430 bp
  70% of sequence in fragments shorter than     486 bp
  75% of sequence in fragments shorter than     558 bp
  80% of sequence in fragments shorter than     647 bp
  85% of sequence in fragments shorter than     767 bp
  90% of sequence in fragments shorter than     965 bp
  95% of sequence in fragments shorter than    1378 bp

# MseI.XhoI (TTAA+CTCGAG) double digest, 19335 thousand fragments (19214674+121308 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     205 bp
  40% of sequence in fragments shorter than     237 bp
  45% of sequence in fragments shorter than     271 bp
  50% of sequence in fragments shorter than     306 bp
  55% of sequence in fragments shorter than     343 bp
  60% of sequence in fragments shorter than     382 bp
  65% of sequence in fragments shorter than     431 bp
  70% of sequence in fragments shorter than     489 bp
  75% of sequence in fragments shorter than     562 bp
  80% of sequence in fragments shorter than     654 bp
  85% of sequence in fragments shorter than     779 bp
  90% of sequence in fragments shorter than     993 bp
  95% of sequence in fragments shorter than    1451 bp

# ClaI.MseI (ATCGAT+TTAA) double digest, 19300 thousand fragments (86477+19214674 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     238 bp
  45% of sequence in fragments shorter than     272 bp
  50% of sequence in fragments shorter than     308 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     384 bp
  65% of sequence in fragments shorter than     434 bp
  70% of sequence in fragments shorter than     492 bp
  75% of sequence in fragments shorter than     566 bp
  80% of sequence in fragments shorter than     660 bp
  85% of sequence in fragments shorter than     788 bp
  90% of sequence in fragments shorter than    1012 bp
  95% of sequence in fragments shorter than    1502 bp

# DdeI.TfiI (CTNAG+GAWTC) double digest, 19295 thousand fragments (14312045+4984362 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      83 bp
  15% of sequence in fragments shorter than     106 bp
  20% of sequence in fragments shorter than     127 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     169 bp
  35% of sequence in fragments shorter than     190 bp
  40% of sequence in fragments shorter than     214 bp
  45% of sequence in fragments shorter than     239 bp
  50% of sequence in fragments shorter than     265 bp
  55% of sequence in fragments shorter than     292 bp
  60% of sequence in fragments shorter than     323 bp
  65% of sequence in fragments shorter than     355 bp
  70% of sequence in fragments shorter than     393 bp
  75% of sequence in fragments shorter than     438 bp
  80% of sequence in fragments shorter than     492 bp
  85% of sequence in fragments shorter than     558 bp
  90% of sequence in fragments shorter than     652 bp
  95% of sequence in fragments shorter than     814 bp

# AatII.MseI (GACGTC+TTAA) double digest, 19285 thousand fragments (71089+19214674 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     238 bp
  45% of sequence in fragments shorter than     273 bp
  50% of sequence in fragments shorter than     308 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     384 bp
  65% of sequence in fragments shorter than     434 bp
  70% of sequence in fragments shorter than     491 bp
  75% of sequence in fragments shorter than     566 bp
  80% of sequence in fragments shorter than     658 bp
  85% of sequence in fragments shorter than     784 bp
  90% of sequence in fragments shorter than    1003 bp
  95% of sequence in fragments shorter than    1473 bp

# MseI.SacII (TTAA+CCGCGG) double digest, 19281 thousand fragments (19214674+66901 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     238 bp
  45% of sequence in fragments shorter than     273 bp
  50% of sequence in fragments shorter than     308 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     384 bp
  65% of sequence in fragments shorter than     433 bp
  70% of sequence in fragments shorter than     490 bp
  75% of sequence in fragments shorter than     563 bp
  80% of sequence in fragments shorter than     654 bp
  85% of sequence in fragments shorter than     778 bp
  90% of sequence in fragments shorter than     986 bp
  95% of sequence in fragments shorter than    1429 bp

# MseI.PspXI (TTAA+VCTCGAGB) double digest, 19273 thousand fragments (19214674+59193 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     238 bp
  45% of sequence in fragments shorter than     273 bp
  50% of sequence in fragments shorter than     308 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     384 bp
  65% of sequence in fragments shorter than     434 bp
  70% of sequence in fragments shorter than     492 bp
  75% of sequence in fragments shorter than     566 bp
  80% of sequence in fragments shorter than     659 bp
  85% of sequence in fragments shorter than     784 bp
  90% of sequence in fragments shorter than    1004 bp
  95% of sequence in fragments shorter than    1473 bp

# AgeI.MseI (ACCGGT+TTAA) double digest, 19267 thousand fragments (53386+19214674 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     150 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     238 bp
  45% of sequence in fragments shorter than     273 bp
  50% of sequence in fragments shorter than     308 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     385 bp
  65% of sequence in fragments shorter than     435 bp
  70% of sequence in fragments shorter than     493 bp
  75% of sequence in fragments shorter than     567 bp
  80% of sequence in fragments shorter than     661 bp
  85% of sequence in fragments shorter than     788 bp
  90% of sequence in fragments shorter than    1010 bp
  95% of sequence in fragments shorter than    1491 bp

# MseI.PmeI (TTAA+GTTTAAAC) double digest, 19256 thousand fragments (19214674+42094 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     310 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     387 bp
  65% of sequence in fragments shorter than     437 bp
  70% of sequence in fragments shorter than     495 bp
  75% of sequence in fragments shorter than     571 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     794 bp
  90% of sequence in fragments shorter than    1022 bp
  95% of sequence in fragments shorter than    1518 bp

# MseI.SalI (TTAA+GTCGAC) double digest, 19245 thousand fragments (19214674+31396 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     273 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     569 bp
  80% of sequence in fragments shorter than     662 bp
  85% of sequence in fragments shorter than     790 bp
  90% of sequence in fragments shorter than    1015 bp
  95% of sequence in fragments shorter than    1501 bp

# AluI.BthCI (AGCT+GCNGC) double digest, 19237 thousand fragments (13027778+6209929 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     114 bp
  20% of sequence in fragments shorter than     141 bp
  25% of sequence in fragments shorter than     168 bp
  30% of sequence in fragments shorter than     194 bp
  35% of sequence in fragments shorter than     219 bp
  40% of sequence in fragments shorter than     246 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     304 bp
  55% of sequence in fragments shorter than     336 bp
  60% of sequence in fragments shorter than     369 bp
  65% of sequence in fragments shorter than     408 bp
  70% of sequence in fragments shorter than     453 bp
  75% of sequence in fragments shorter than     506 bp
  80% of sequence in fragments shorter than     570 bp
  85% of sequence in fragments shorter than     654 bp
  90% of sequence in fragments shorter than     769 bp
  95% of sequence in fragments shorter than     975 bp

# MluI.MseI (ACGCGT+TTAA) double digest, 19235 thousand fragments (21415+19214674 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     569 bp
  80% of sequence in fragments shorter than     663 bp
  85% of sequence in fragments shorter than     791 bp
  90% of sequence in fragments shorter than    1015 bp
  95% of sequence in fragments shorter than    1499 bp

# MseI.SgrAI (TTAA+CRCCGGYG) double digest, 19229 thousand fragments (19214674+15270 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     569 bp
  80% of sequence in fragments shorter than     663 bp
  85% of sequence in fragments shorter than     790 bp
  90% of sequence in fragments shorter than    1012 bp
  95% of sequence in fragments shorter than    1490 bp

# MseI.NruI (TTAA+TCGCGA) double digest, 19229 thousand fragments (19214674+14872 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     569 bp
  80% of sequence in fragments shorter than     663 bp
  85% of sequence in fragments shorter than     791 bp
  90% of sequence in fragments shorter than    1017 bp
  95% of sequence in fragments shorter than    1505 bp

# MseI.PvuI (TTAA+CGATCG) double digest, 19228 thousand fragments (19214674+14186 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     570 bp
  80% of sequence in fragments shorter than     664 bp
  85% of sequence in fragments shorter than     791 bp
  90% of sequence in fragments shorter than    1016 bp
  95% of sequence in fragments shorter than    1498 bp

# FseI.MseI (GGCCGGCC+TTAA) double digest, 19227 thousand fragments (13662+19214674 sites), 154 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     569 bp
  80% of sequence in fragments shorter than     663 bp
  85% of sequence in fragments shorter than     790 bp
  90% of sequence in fragments shorter than    1013 bp
  95% of sequence in fragments shorter than    1491 bp

# MseI.NotI (TTAA+GCGGCCGC) double digest, 19223 thousand fragments (19214674+9795 sites), 155 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     570 bp
  80% of sequence in fragments shorter than     663 bp
  85% of sequence in fragments shorter than     791 bp
  90% of sequence in fragments shorter than    1014 bp
  95% of sequence in fragments shorter than    1495 bp

# MseI.RsrII (TTAA+CGGWCCG) double digest, 19223 thousand fragments (19214674+9764 sites), 155 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     570 bp
  80% of sequence in fragments shorter than     664 bp
  85% of sequence in fragments shorter than     791 bp
  90% of sequence in fragments shorter than    1016 bp
  95% of sequence in fragments shorter than    1500 bp

# AscI.MseI (GGCGCGCC+TTAA) double digest, 19218 thousand fragments (4751+19214674 sites), 155 bp average fragment length
   5% of sequence in fragments shorter than      47 bp
  10% of sequence in fragments shorter than      75 bp
  15% of sequence in fragments shorter than     100 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     274 bp
  50% of sequence in fragments shorter than     310 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     387 bp
  65% of sequence in fragments shorter than     437 bp
  70% of sequence in fragments shorter than     495 bp
  75% of sequence in fragments shorter than     570 bp
  80% of sequence in fragments shorter than     664 bp
  85% of sequence in fragments shorter than     793 bp
  90% of sequence in fragments shorter than    1018 bp
  95% of sequence in fragments shorter than    1506 bp

# AluI.BccI (AGCT+CCATC) double digest, 19198 thousand fragments (13027778+6170927 sites), 155 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      78 bp
  15% of sequence in fragments shorter than     102 bp
  20% of sequence in fragments shorter than     125 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     170 bp
  35% of sequence in fragments shorter than     193 bp
  40% of sequence in fragments shorter than     216 bp
  45% of sequence in fragments shorter than     241 bp
  50% of sequence in fragments shorter than     267 bp
  55% of sequence in fragments shorter than     296 bp
  60% of sequence in fragments shorter than     326 bp
  65% of sequence in fragments shorter than     359 bp
  70% of sequence in fragments shorter than     398 bp
  75% of sequence in fragments shorter than     444 bp
  80% of sequence in fragments shorter than     499 bp
  85% of sequence in fragments shorter than     572 bp
  90% of sequence in fragments shorter than     676 bp
  95% of sequence in fragments shorter than     856 bp

# BbvI.CviAII (GCAGC+CATG) double digest, 19105 thousand fragments (5290051+13815697 sites), 155 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      81 bp
  15% of sequence in fragments shorter than     106 bp
  20% of sequence in fragments shorter than     130 bp
  25% of sequence in fragments shorter than     153 bp
  30% of sequence in fragments shorter than     178 bp
  35% of sequence in fragments shorter than     203 bp
  40% of sequence in fragments shorter than     228 bp
  45% of sequence in fragments shorter than     254 bp
  50% of sequence in fragments shorter than     282 bp
  55% of sequence in fragments shorter than     311 bp
  60% of sequence in fragments shorter than     343 bp
  65% of sequence in fragments shorter than     380 bp
  70% of sequence in fragments shorter than     421 bp
  75% of sequence in fragments shorter than     471 bp
  80% of sequence in fragments shorter than     532 bp
  85% of sequence in fragments shorter than     609 bp
  90% of sequence in fragments shorter than     718 bp
  95% of sequence in fragments shorter than     903 bp

# BspNCI.ScrFI (CCAGA+CCNGG) double digest, 19087 thousand fragments (7282436+11805099 sites), 156 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      70 bp
  15% of sequence in fragments shorter than      94 bp
  20% of sequence in fragments shorter than     119 bp
  25% of sequence in fragments shorter than     145 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     205 bp
  40% of sequence in fragments shorter than     237 bp
  45% of sequence in fragments shorter than     273 bp
  50% of sequence in fragments shorter than     313 bp
  55% of sequence in fragments shorter than     352 bp
  60% of sequence in fragments shorter than     399 bp
  65% of sequence in fragments shorter than     454 bp
  70% of sequence in fragments shorter than     517 bp
  75% of sequence in fragments shorter than     590 bp
  80% of sequence in fragments shorter than     683 bp
  85% of sequence in fragments shorter than     796 bp
  90% of sequence in fragments shorter than     967 bp
  95% of sequence in fragments shorter than    1249 bp

# BstNI.MboII (CCWGG+GAAGA) double digest, 19054 thousand fragments (9855646+9199496 sites), 156 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      79 bp
  15% of sequence in fragments shorter than     103 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     147 bp
  30% of sequence in fragments shorter than     169 bp
  35% of sequence in fragments shorter than     190 bp
  40% of sequence in fragments shorter than     216 bp
  45% of sequence in fragments shorter than     244 bp
  50% of sequence in fragments shorter than     273 bp
  55% of sequence in fragments shorter than     305 bp
  60% of sequence in fragments shorter than     338 bp
  65% of sequence in fragments shorter than     375 bp
  70% of sequence in fragments shorter than     419 bp
  75% of sequence in fragments shorter than     470 bp
  80% of sequence in fragments shorter than     532 bp
  85% of sequence in fragments shorter than     613 bp
  90% of sequence in fragments shorter than     726 bp
  95% of sequence in fragments shorter than     924 bp

# AluI.HphI (AGCT+GGTGA) double digest, 19034 thousand fragments (13027778+6007336 sites), 156 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      76 bp
  15% of sequence in fragments shorter than     101 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     148 bp
  30% of sequence in fragments shorter than     171 bp
  35% of sequence in fragments shorter than     194 bp
  40% of sequence in fragments shorter than     218 bp
  45% of sequence in fragments shorter than     243 bp
  50% of sequence in fragments shorter than     270 bp
  55% of sequence in fragments shorter than     299 bp
  60% of sequence in fragments shorter than     331 bp
  65% of sequence in fragments shorter than     364 bp
  70% of sequence in fragments shorter than     404 bp
  75% of sequence in fragments shorter than     451 bp
  80% of sequence in fragments shorter than     506 bp
  85% of sequence in fragments shorter than     580 bp
  90% of sequence in fragments shorter than     686 bp
  95% of sequence in fragments shorter than     871 bp

# CviAII.FokI (CATG+GGATG) double digest, 19016 thousand fragments (13815697+5201116 sites), 156 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      81 bp
  15% of sequence in fragments shorter than     105 bp
  20% of sequence in fragments shorter than     128 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     198 bp
  40% of sequence in fragments shorter than     223 bp
  45% of sequence in fragments shorter than     248 bp
  50% of sequence in fragments shorter than     275 bp
  55% of sequence in fragments shorter than     304 bp
  60% of sequence in fragments shorter than     334 bp
  65% of sequence in fragments shorter than     369 bp
  70% of sequence in fragments shorter than     409 bp
  75% of sequence in fragments shorter than     456 bp
  80% of sequence in fragments shorter than     514 bp
  85% of sequence in fragments shorter than     586 bp
  90% of sequence in fragments shorter than     691 bp
  95% of sequence in fragments shorter than     872 bp

# BspKT6I.ScrFI (GATC+CCNGG) double digest, 19004 thousand fragments (7199434+11805099 sites), 156 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      71 bp
  15% of sequence in fragments shorter than     101 bp
  20% of sequence in fragments shorter than     127 bp
  25% of sequence in fragments shorter than     155 bp
  30% of sequence in fragments shorter than     185 bp
  35% of sequence in fragments shorter than     216 bp
  40% of sequence in fragments shorter than     251 bp
  45% of sequence in fragments shorter than     287 bp
  50% of sequence in fragments shorter than     326 bp
  55% of sequence in fragments shorter than     367 bp
  60% of sequence in fragments shorter than     414 bp
  65% of sequence in fragments shorter than     465 bp
  70% of sequence in fragments shorter than     527 bp
  75% of sequence in fragments shorter than     601 bp
  80% of sequence in fragments shorter than     689 bp
  85% of sequence in fragments shorter than     805 bp
  90% of sequence in fragments shorter than     973 bp
  95% of sequence in fragments shorter than    1282 bp

# CviAII.CviQI (CATG+GTAC) double digest, 18930 thousand fragments (13815697+5115078 sites), 157 bp average fragment length
   5% of sequence in fragments shorter than      54 bp
  10% of sequence in fragments shorter than      82 bp
  15% of sequence in fragments shorter than     106 bp
  20% of sequence in fragments shorter than     130 bp
  25% of sequence in fragments shorter than     153 bp
  30% of sequence in fragments shorter than     176 bp
  35% of sequence in fragments shorter than     200 bp
  40% of sequence in fragments shorter than     224 bp
  45% of sequence in fragments shorter than     249 bp
  50% of sequence in fragments shorter than     276 bp
  55% of sequence in fragments shorter than     303 bp
  60% of sequence in fragments shorter than     334 bp
  65% of sequence in fragments shorter than     369 bp
  70% of sequence in fragments shorter than     409 bp
  75% of sequence in fragments shorter than     457 bp
  80% of sequence in fragments shorter than     514 bp
  85% of sequence in fragments shorter than     586 bp
  90% of sequence in fragments shorter than     688 bp
  95% of sequence in fragments shorter than     872 bp

# Hin4II.ScrFI (CCTTC+CCNGG) double digest, 18864 thousand fragments (7059915+11805099 sites), 157 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      73 bp
  15% of sequence in fragments shorter than      99 bp
  20% of sequence in fragments shorter than     124 bp
  25% of sequence in fragments shorter than     152 bp
  30% of sequence in fragments shorter than     181 bp
  35% of sequence in fragments shorter than     212 bp
  40% of sequence in fragments shorter than     246 bp
  45% of sequence in fragments shorter than     282 bp
  50% of sequence in fragments shorter than     321 bp
  55% of sequence in fragments shorter than     362 bp
  60% of sequence in fragments shorter than     411 bp
  65% of sequence in fragments shorter than     466 bp
  70% of sequence in fragments shorter than     529 bp
  75% of sequence in fragments shorter than     606 bp
  80% of sequence in fragments shorter than     698 bp
  85% of sequence in fragments shorter than     824 bp
  90% of sequence in fragments shorter than    1000 bp
  95% of sequence in fragments shorter than    1293 bp

# CviAII.TfiI (CATG+GAWTC) double digest, 18799 thousand fragments (13815697+4984362 sites), 158 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      82 bp
  15% of sequence in fragments shorter than     105 bp
  20% of sequence in fragments shorter than     129 bp
  25% of sequence in fragments shorter than     151 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     195 bp
  40% of sequence in fragments shorter than     219 bp
  45% of sequence in fragments shorter than     245 bp
  50% of sequence in fragments shorter than     271 bp
  55% of sequence in fragments shorter than     299 bp
  60% of sequence in fragments shorter than     330 bp
  65% of sequence in fragments shorter than     360 bp
  70% of sequence in fragments shorter than     398 bp
  75% of sequence in fragments shorter than     442 bp
  80% of sequence in fragments shorter than     496 bp
  85% of sequence in fragments shorter than     564 bp
  90% of sequence in fragments shorter than     660 bp
  95% of sequence in fragments shorter than     820 bp

# BstNI.Hpy188I (CCWGG+TCNGA) double digest, 18797 thousand fragments (9855646+8942153 sites), 158 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      77 bp
  15% of sequence in fragments shorter than     102 bp
  20% of sequence in fragments shorter than     122 bp
  25% of sequence in fragments shorter than     146 bp
  30% of sequence in fragments shorter than     169 bp
  35% of sequence in fragments shorter than     189 bp
  40% of sequence in fragments shorter than     215 bp
  45% of sequence in fragments shorter than     243 bp
  50% of sequence in fragments shorter than     273 bp
  55% of sequence in fragments shorter than     306 bp
  60% of sequence in fragments shorter than     341 bp
  65% of sequence in fragments shorter than     378 bp
  70% of sequence in fragments shorter than     423 bp
  75% of sequence in fragments shorter than     477 bp
  80% of sequence in fragments shorter than     544 bp
  85% of sequence in fragments shorter than     629 bp
  90% of sequence in fragments shorter than     746 bp
  95% of sequence in fragments shorter than     948 bp

# BstNI.HinfI (CCWGG+GANTC) double digest, 18771 thousand fragments (9855646+8916054 sites), 158 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      77 bp
  15% of sequence in fragments shorter than     104 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     152 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     204 bp
  40% of sequence in fragments shorter than     234 bp
  45% of sequence in fragments shorter than     265 bp
  50% of sequence in fragments shorter than     298 bp
  55% of sequence in fragments shorter than     332 bp
  60% of sequence in fragments shorter than     365 bp
  65% of sequence in fragments shorter than     408 bp
  70% of sequence in fragments shorter than     460 bp
  75% of sequence in fragments shorter than     521 bp
  80% of sequence in fragments shorter than     596 bp
  85% of sequence in fragments shorter than     690 bp
  90% of sequence in fragments shorter than     819 bp
  95% of sequence in fragments shorter than    1043 bp

# AluI.BsrI (AGCT+ACTGG) double digest, 18768 thousand fragments (13027778+5741310 sites), 158 bp average fragment length
   5% of sequence in fragments shorter than      52 bp
  10% of sequence in fragments shorter than      80 bp
  15% of sequence in fragments shorter than     105 bp
  20% of sequence in fragments shorter than     129 bp
  25% of sequence in fragments shorter than     152 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     196 bp
  40% of sequence in fragments shorter than     219 bp
  45% of sequence in fragments shorter than     242 bp
  50% of sequence in fragments shorter than     268 bp
  55% of sequence in fragments shorter than     296 bp
  60% of sequence in fragments shorter than     326 bp
  65% of sequence in fragments shorter than     357 bp
  70% of sequence in fragments shorter than     396 bp
  75% of sequence in fragments shorter than     441 bp
  80% of sequence in fragments shorter than     495 bp
  85% of sequence in fragments shorter than     564 bp
  90% of sequence in fragments shorter than     663 bp
  95% of sequence in fragments shorter than     839 bp

# BstNI.TspRI (CCWGG+CASTG) double digest, 18620 thousand fragments (9855646+8765240 sites), 160 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      79 bp
  15% of sequence in fragments shorter than     106 bp
  20% of sequence in fragments shorter than     130 bp
  25% of sequence in fragments shorter than     155 bp
  30% of sequence in fragments shorter than     184 bp
  35% of sequence in fragments shorter than     213 bp
  40% of sequence in fragments shorter than     246 bp
  45% of sequence in fragments shorter than     280 bp
  50% of sequence in fragments shorter than     319 bp
  55% of sequence in fragments shorter than     357 bp
  60% of sequence in fragments shorter than     403 bp
  65% of sequence in fragments shorter than     455 bp
  70% of sequence in fragments shorter than     516 bp
  75% of sequence in fragments shorter than     587 bp
  80% of sequence in fragments shorter than     675 bp
  85% of sequence in fragments shorter than     790 bp
  90% of sequence in fragments shorter than     951 bp
  95% of sequence in fragments shorter than    1258 bp

# AciI.DdeI (CCGC+CTNAG) double digest, 18517 thousand fragments (4206130+14312045 sites), 160 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     111 bp
  20% of sequence in fragments shorter than     133 bp
  25% of sequence in fragments shorter than     158 bp
  30% of sequence in fragments shorter than     179 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     237 bp
  45% of sequence in fragments shorter than     267 bp
  50% of sequence in fragments shorter than     299 bp
  55% of sequence in fragments shorter than     334 bp
  60% of sequence in fragments shorter than     371 bp
  65% of sequence in fragments shorter than     413 bp
  70% of sequence in fragments shorter than     463 bp
  75% of sequence in fragments shorter than     520 bp
  80% of sequence in fragments shorter than     589 bp
  85% of sequence in fragments shorter than     680 bp
  90% of sequence in fragments shorter than     804 bp
  95% of sequence in fragments shorter than    1025 bp

# BsmAI.ScrFI (GTCTC+CCNGG) double digest, 18436 thousand fragments (6631585+11805099 sites), 161 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      74 bp
  15% of sequence in fragments shorter than     102 bp
  20% of sequence in fragments shorter than     132 bp
  25% of sequence in fragments shorter than     165 bp
  30% of sequence in fragments shorter than     201 bp
  35% of sequence in fragments shorter than     240 bp
  40% of sequence in fragments shorter than     281 bp
  45% of sequence in fragments shorter than     325 bp
  50% of sequence in fragments shorter than     374 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     488 bp
  65% of sequence in fragments shorter than     559 bp
  70% of sequence in fragments shorter than     640 bp
  75% of sequence in fragments shorter than     737 bp
  80% of sequence in fragments shorter than     861 bp
  85% of sequence in fragments shorter than    1026 bp
  90% of sequence in fragments shorter than    1272 bp
  95% of sequence in fragments shorter than    1745 bp

# BstNI.HaeIII (CCWGG+GGCC) double digest, 18417 thousand fragments (9855646+8562230 sites), 161 bp average fragment length
   5% of sequence in fragments shorter than      49 bp
  10% of sequence in fragments shorter than      80 bp
  15% of sequence in fragments shorter than     113 bp
  20% of sequence in fragments shorter than     140 bp
  25% of sequence in fragments shorter than     171 bp
  30% of sequence in fragments shorter than     207 bp
  35% of sequence in fragments shorter than     245 bp
  40% of sequence in fragments shorter than     289 bp
  45% of sequence in fragments shorter than     336 bp
  50% of sequence in fragments shorter than     379 bp
  55% of sequence in fragments shorter than     435 bp
  60% of sequence in fragments shorter than     499 bp
  65% of sequence in fragments shorter than     570 bp
  70% of sequence in fragments shorter than     651 bp
  75% of sequence in fragments shorter than     745 bp
  80% of sequence in fragments shorter than     863 bp
  85% of sequence in fragments shorter than    1015 bp
  90% of sequence in fragments shorter than    1230 bp
  95% of sequence in fragments shorter than    1606 bp

# DdeI.Tsp45I (CTNAG+GTSAC) double digest, 18333 thousand fragments (14312045+4021761 sites), 162 bp average fragment length
   5% of sequence in fragments shorter than      55 bp
  10% of sequence in fragments shorter than      84 bp
  15% of sequence in fragments shorter than     110 bp
  20% of sequence in fragments shorter than     132 bp
  25% of sequence in fragments shorter than     154 bp
  30% of sequence in fragments shorter than     174 bp
  35% of sequence in fragments shorter than     199 bp
  40% of sequence in fragments shorter than     224 bp
  45% of sequence in fragments shorter than     251 bp
  50% of sequence in fragments shorter than     280 bp
  55% of sequence in fragments shorter than     310 bp
  60% of sequence in fragments shorter than     343 bp
  65% of sequence in fragments shorter than     379 bp
  70% of sequence in fragments shorter than     420 bp
  75% of sequence in fragments shorter than     471 bp
  80% of sequence in fragments shorter than     533 bp
  85% of sequence in fragments shorter than     610 bp
  90% of sequence in fragments shorter than     724 bp
  95% of sequence in fragments shorter than     919 bp

# AluI.BbvI (AGCT+GCAGC) double digest, 18317 thousand fragments (13027778+5290051 sites), 162 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      90 bp
  15% of sequence in fragments shorter than     120 bp
  20% of sequence in fragments shorter than     148 bp
  25% of sequence in fragments shorter than     175 bp
  30% of sequence in fragments shorter than     201 bp
  35% of sequence in fragments shorter than     226 bp
  40% of sequence in fragments shorter than     254 bp
  45% of sequence in fragments shorter than     282 bp
  50% of sequence in fragments shorter than     312 bp
  55% of sequence in fragments shorter than     343 bp
  60% of sequence in fragments shorter than     378 bp
  65% of sequence in fragments shorter than     419 bp
  70% of sequence in fragments shorter than     464 bp
  75% of sequence in fragments shorter than     518 bp
  80% of sequence in fragments shorter than     582 bp
  85% of sequence in fragments shorter than     668 bp
  90% of sequence in fragments shorter than     787 bp
  95% of sequence in fragments shorter than    1001 bp

# DdeI.MlyI (CTNAG+GAGTC) double digest, 18243 thousand fragments (14312045+3931692 sites), 163 bp average fragment length
   5% of sequence in fragments shorter than      56 bp
  10% of sequence in fragments shorter than      88 bp
  15% of sequence in fragments shorter than     111 bp
  20% of sequence in fragments shorter than     133 bp
  25% of sequence in fragments shorter than     157 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     204 bp
  40% of sequence in fragments shorter than     230 bp
  45% of sequence in fragments shorter than     258 bp
  50% of sequence in fragments shorter than     287 bp
  55% of sequence in fragments shorter than     318 bp
  60% of sequence in fragments shorter than     351 bp
  65% of sequence in fragments shorter than     391 bp
  70% of sequence in fragments shorter than     434 bp
  75% of sequence in fragments shorter than     486 bp
  80% of sequence in fragments shorter than     549 bp
  85% of sequence in fragments shorter than     631 bp
  90% of sequence in fragments shorter than     741 bp
  95% of sequence in fragments shorter than     935 bp

# AluI.FokI (AGCT+GGATG) double digest, 18228 thousand fragments (13027778+5201116 sites), 163 bp average fragment length
   5% of sequence in fragments shorter than      54 bp
  10% of sequence in fragments shorter than      83 bp
  15% of sequence in fragments shorter than     108 bp
  20% of sequence in fragments shorter than     133 bp
  25% of sequence in fragments shorter than     157 bp
  30% of sequence in fragments shorter than     180 bp
  35% of sequence in fragments shorter than     203 bp
  40% of sequence in fragments shorter than     227 bp
  45% of sequence in fragments shorter than     252 bp
  50% of sequence in fragments shorter than     280 bp
  55% of sequence in fragments shorter than     310 bp
  60% of sequence in fragments shorter than     341 bp
  65% of sequence in fragments shorter than     374 bp
  70% of sequence in fragments shorter than     415 bp
  75% of sequence in fragments shorter than     461 bp
  80% of sequence in fragments shorter than     518 bp
  85% of sequence in fragments shorter than     591 bp
  90% of sequence in fragments shorter than     694 bp
  95% of sequence in fragments shorter than     878 bp

# ApoI.ScrFI (RAATTY+CCNGG) double digest, 18187 thousand fragments (6382433+11805099 sites), 163 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      79 bp
  15% of sequence in fragments shorter than     104 bp
  20% of sequence in fragments shorter than     126 bp
  25% of sequence in fragments shorter than     153 bp
  30% of sequence in fragments shorter than     179 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     234 bp
  45% of sequence in fragments shorter than     263 bp
  50% of sequence in fragments shorter than     295 bp
  55% of sequence in fragments shorter than     331 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     411 bp
  70% of sequence in fragments shorter than     460 bp
  75% of sequence in fragments shorter than     517 bp
  80% of sequence in fragments shorter than     588 bp
  85% of sequence in fragments shorter than     681 bp
  90% of sequence in fragments shorter than     810 bp
  95% of sequence in fragments shorter than    1050 bp

# AluI.CviQI (AGCT+GTAC) double digest, 18142 thousand fragments (13027778+5115078 sites), 164 bp average fragment length
   5% of sequence in fragments shorter than      55 bp
  10% of sequence in fragments shorter than      84 bp
  15% of sequence in fragments shorter than     109 bp
  20% of sequence in fragments shorter than     135 bp
  25% of sequence in fragments shorter than     158 bp
  30% of sequence in fragments shorter than     180 bp
  35% of sequence in fragments shorter than     203 bp
  40% of sequence in fragments shorter than     225 bp
  45% of sequence in fragments shorter than     250 bp
  50% of sequence in fragments shorter than     275 bp
  55% of sequence in fragments shorter than     302 bp
  60% of sequence in fragments shorter than     332 bp
  65% of sequence in fragments shorter than     363 bp
  70% of sequence in fragments shorter than     400 bp
  75% of sequence in fragments shorter than     444 bp
  80% of sequence in fragments shorter than     497 bp
  85% of sequence in fragments shorter than     565 bp
  90% of sequence in fragments shorter than     664 bp
  95% of sequence in fragments shorter than     832 bp

# Hpy188I.MboII (TCNGA+GAAGA) double digest, 18141 thousand fragments (8942153+9199496 sites), 164 bp average fragment length
   5% of sequence in fragments shorter than      55 bp
  10% of sequence in fragments shorter than      86 bp
  15% of sequence in fragments shorter than     112 bp
  20% of sequence in fragments shorter than     137 bp
  25% of sequence in fragments shorter than     159 bp
  30% of sequence in fragments shorter than     183 bp
  35% of sequence in fragments shorter than     210 bp
  40% of sequence in fragments shorter than     238 bp
  45% of sequence in fragments shorter than     268 bp
  50% of sequence in fragments shorter than     300 bp
  55% of sequence in fragments shorter than     334 bp
  60% of sequence in fragments shorter than     372 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     464 bp
  75% of sequence in fragments shorter than     520 bp
  80% of sequence in fragments shorter than     589 bp
  85% of sequence in fragments shorter than     677 bp
  90% of sequence in fragments shorter than     800 bp
  95% of sequence in fragments shorter than     999 bp

# HinfI.MboII (GANTC+GAAGA) double digest, 18115 thousand fragments (8916054+9199496 sites), 164 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     113 bp
  20% of sequence in fragments shorter than     138 bp
  25% of sequence in fragments shorter than     160 bp
  30% of sequence in fragments shorter than     184 bp
  35% of sequence in fragments shorter than     210 bp
  40% of sequence in fragments shorter than     234 bp
  45% of sequence in fragments shorter than     261 bp
  50% of sequence in fragments shorter than     289 bp
  55% of sequence in fragments shorter than     318 bp
  60% of sequence in fragments shorter than     350 bp
  65% of sequence in fragments shorter than     387 bp
  70% of sequence in fragments shorter than     428 bp
  75% of sequence in fragments shorter than     476 bp
  80% of sequence in fragments shorter than     534 bp
  85% of sequence in fragments shorter than     606 bp
  90% of sequence in fragments shorter than     702 bp
  95% of sequence in fragments shorter than     868 bp

# AciI.CviAII (CCGC+CATG) double digest, 18021 thousand fragments (4206130+13815697 sites), 165 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      83 bp
  15% of sequence in fragments shorter than     111 bp
  20% of sequence in fragments shorter than     138 bp
  25% of sequence in fragments shorter than     164 bp
  30% of sequence in fragments shorter than     191 bp
  35% of sequence in fragments shorter than     219 bp
  40% of sequence in fragments shorter than     247 bp
  45% of sequence in fragments shorter than     276 bp
  50% of sequence in fragments shorter than     306 bp
  55% of sequence in fragments shorter than     339 bp
  60% of sequence in fragments shorter than     375 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     463 bp
  75% of sequence in fragments shorter than     517 bp
  80% of sequence in fragments shorter than     583 bp
  85% of sequence in fragments shorter than     666 bp
  90% of sequence in fragments shorter than     784 bp
  95% of sequence in fragments shorter than     982 bp

# BthCI.ScrFI (GCNGC+CCNGG) double digest, 18014 thousand fragments (6209929+11805099 sites), 165 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      77 bp
  15% of sequence in fragments shorter than     107 bp
  20% of sequence in fragments shorter than     136 bp
  25% of sequence in fragments shorter than     171 bp
  30% of sequence in fragments shorter than     208 bp
  35% of sequence in fragments shorter than     249 bp
  40% of sequence in fragments shorter than     291 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     389 bp
  55% of sequence in fragments shorter than     446 bp
  60% of sequence in fragments shorter than     512 bp
  65% of sequence in fragments shorter than     586 bp
  70% of sequence in fragments shorter than     668 bp
  75% of sequence in fragments shorter than     765 bp
  80% of sequence in fragments shorter than     887 bp
  85% of sequence in fragments shorter than    1049 bp
  90% of sequence in fragments shorter than    1271 bp
  95% of sequence in fragments shorter than    1666 bp

# AluI.TfiI (AGCT+GAWTC) double digest, 18011 thousand fragments (13027778+4984362 sites), 165 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     112 bp
  20% of sequence in fragments shorter than     137 bp
  25% of sequence in fragments shorter than     160 bp
  30% of sequence in fragments shorter than     183 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     230 bp
  45% of sequence in fragments shorter than     256 bp
  50% of sequence in fragments shorter than     281 bp
  55% of sequence in fragments shorter than     310 bp
  60% of sequence in fragments shorter than     339 bp
  65% of sequence in fragments shorter than     370 bp
  70% of sequence in fragments shorter than     410 bp
  75% of sequence in fragments shorter than     456 bp
  80% of sequence in fragments shorter than     510 bp
  85% of sequence in fragments shorter than     580 bp
  90% of sequence in fragments shorter than     678 bp
  95% of sequence in fragments shorter than     843 bp

# BccI.ScrFI (CCATC+CCNGG) double digest, 17975 thousand fragments (6170927+11805099 sites), 165 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      77 bp
  15% of sequence in fragments shorter than     104 bp
  20% of sequence in fragments shorter than     131 bp
  25% of sequence in fragments shorter than     162 bp
  30% of sequence in fragments shorter than     193 bp
  35% of sequence in fragments shorter than     227 bp
  40% of sequence in fragments shorter than     265 bp
  45% of sequence in fragments shorter than     305 bp
  50% of sequence in fragments shorter than     346 bp
  55% of sequence in fragments shorter than     394 bp
  60% of sequence in fragments shorter than     447 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     581 bp
  75% of sequence in fragments shorter than     665 bp
  80% of sequence in fragments shorter than     771 bp
  85% of sequence in fragments shorter than     912 bp
  90% of sequence in fragments shorter than    1120 bp
  95% of sequence in fragments shorter than    1519 bp

# BscAI.DdeI (GCATC+CTNAG) double digest, 17964 thousand fragments (3652926+14312045 sites), 165 bp average fragment length
   5% of sequence in fragments shorter than      56 bp
  10% of sequence in fragments shorter than      88 bp
  15% of sequence in fragments shorter than     114 bp
  20% of sequence in fragments shorter than     135 bp
  25% of sequence in fragments shorter than     158 bp
  30% of sequence in fragments shorter than     177 bp
  35% of sequence in fragments shorter than     202 bp
  40% of sequence in fragments shorter than     228 bp
  45% of sequence in fragments shorter than     254 bp
  50% of sequence in fragments shorter than     283 bp
  55% of sequence in fragments shorter than     312 bp
  60% of sequence in fragments shorter than     344 bp
  65% of sequence in fragments shorter than     380 bp
  70% of sequence in fragments shorter than     419 bp
  75% of sequence in fragments shorter than     467 bp
  80% of sequence in fragments shorter than     525 bp
  85% of sequence in fragments shorter than     600 bp
  90% of sequence in fragments shorter than     705 bp
  95% of sequence in fragments shorter than     877 bp

# MboII.TspRI (GAAGA+CASTG) double digest, 17964 thousand fragments (9199496+8765240 sites), 165 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      89 bp
  15% of sequence in fragments shorter than     116 bp
  20% of sequence in fragments shorter than     140 bp
  25% of sequence in fragments shorter than     164 bp
  30% of sequence in fragments shorter than     186 bp
  35% of sequence in fragments shorter than     211 bp
  40% of sequence in fragments shorter than     233 bp
  45% of sequence in fragments shorter than     258 bp
  50% of sequence in fragments shorter than     285 bp
  55% of sequence in fragments shorter than     313 bp
  60% of sequence in fragments shorter than     343 bp
  65% of sequence in fragments shorter than     377 bp
  70% of sequence in fragments shorter than     417 bp
  75% of sequence in fragments shorter than     462 bp
  80% of sequence in fragments shorter than     516 bp
  85% of sequence in fragments shorter than     586 bp
  90% of sequence in fragments shorter than     685 bp
  95% of sequence in fragments shorter than     850 bp

# HinfI.Hpy188I (GANTC+TCNGA) double digest, 17857 thousand fragments (8916054+8942153 sites), 166 bp average fragment length
   5% of sequence in fragments shorter than      60 bp
  10% of sequence in fragments shorter than      87 bp
  15% of sequence in fragments shorter than     115 bp
  20% of sequence in fragments shorter than     141 bp
  25% of sequence in fragments shorter than     167 bp
  30% of sequence in fragments shorter than     191 bp
  35% of sequence in fragments shorter than     218 bp
  40% of sequence in fragments shorter than     244 bp
  45% of sequence in fragments shorter than     272 bp
  50% of sequence in fragments shorter than     300 bp
  55% of sequence in fragments shorter than     330 bp
  60% of sequence in fragments shorter than     363 bp
  65% of sequence in fragments shorter than     401 bp
  70% of sequence in fragments shorter than     443 bp
  75% of sequence in fragments shorter than     493 bp
  80% of sequence in fragments shorter than     553 bp
  85% of sequence in fragments shorter than     629 bp
  90% of sequence in fragments shorter than     730 bp
  95% of sequence in fragments shorter than     903 bp

# DdeI.SfeI (CTNAG+CTRYAG) double digest, 17842 thousand fragments (14312045+3531009 sites), 167 bp average fragment length
   5% of sequence in fragments shorter than      55 bp
  10% of sequence in fragments shorter than      87 bp
  15% of sequence in fragments shorter than     113 bp
  20% of sequence in fragments shorter than     135 bp
  25% of sequence in fragments shorter than     160 bp
  30% of sequence in fragments shorter than     180 bp
  35% of sequence in fragments shorter than     206 bp
  40% of sequence in fragments shorter than     232 bp
  45% of sequence in fragments shorter than     260 bp
  50% of sequence in fragments shorter than     288 bp
  55% of sequence in fragments shorter than     318 bp
  60% of sequence in fragments shorter than     350 bp
  65% of sequence in fragments shorter than     388 bp
  70% of sequence in fragments shorter than     429 bp
  75% of sequence in fragments shorter than     478 bp
  80% of sequence in fragments shorter than     539 bp
  85% of sequence in fragments shorter than     615 bp
  90% of sequence in fragments shorter than     723 bp
  95% of sequence in fragments shorter than     913 bp

# CviAII.Tsp45I (CATG+GTSAC) double digest, 17836 thousand fragments (13815697+4021761 sites), 167 bp average fragment length
   5% of sequence in fragments shorter than      56 bp
  10% of sequence in fragments shorter than      86 bp
  15% of sequence in fragments shorter than     111 bp
  20% of sequence in fragments shorter than     136 bp
  25% of sequence in fragments shorter than     160 bp
  30% of sequence in fragments shorter than     184 bp
  35% of sequence in fragments shorter than     210 bp
  40% of sequence in fragments shorter than     236 bp
  45% of sequence in fragments shorter than     262 bp
  50% of sequence in fragments shorter than     290 bp
  55% of sequence in fragments shorter than     319 bp
  60% of sequence in fragments shorter than     351 bp
  65% of sequence in fragments shorter than     388 bp
  70% of sequence in fragments shorter than     431 bp
  75% of sequence in fragments shorter than     481 bp
  80% of sequence in fragments shorter than     543 bp
  85% of sequence in fragments shorter than     621 bp
  90% of sequence in fragments shorter than     731 bp
  95% of sequence in fragments shorter than     935 bp

# HphI.ScrFI (GGTGA+CCNGG) double digest, 17811 thousand fragments (6007336+11805099 sites), 167 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      78 bp
  15% of sequence in fragments shorter than     107 bp
  20% of sequence in fragments shorter than     135 bp
  25% of sequence in fragments shorter than     168 bp
  30% of sequence in fragments shorter than     203 bp
  35% of sequence in fragments shorter than     241 bp
  40% of sequence in fragments shorter than     281 bp
  45% of sequence in fragments shorter than     323 bp
  50% of sequence in fragments shorter than     368 bp
  55% of sequence in fragments shorter than     419 bp
  60% of sequence in fragments shorter than     477 bp
  65% of sequence in fragments shorter than     541 bp
  70% of sequence in fragments shorter than     615 bp
  75% of sequence in fragments shorter than     702 bp
  80% of sequence in fragments shorter than     815 bp
  85% of sequence in fragments shorter than     961 bp
  90% of sequence in fragments shorter than    1176 bp
  95% of sequence in fragments shorter than    1539 bp

# HaeIII.MboII (GGCC+GAAGA) double digest, 17761 thousand fragments (8562230+9199496 sites), 167 bp average fragment length
   5% of sequence in fragments shorter than      52 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     113 bp
  20% of sequence in fragments shorter than     141 bp
  25% of sequence in fragments shorter than     165 bp
  30% of sequence in fragments shorter than     191 bp
  35% of sequence in fragments shorter than     219 bp
  40% of sequence in fragments shorter than     248 bp
  45% of sequence in fragments shorter than     278 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     339 bp
  60% of sequence in fragments shorter than     375 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     461 bp
  75% of sequence in fragments shorter than     514 bp
  80% of sequence in fragments shorter than     579 bp
  85% of sequence in fragments shorter than     663 bp
  90% of sequence in fragments shorter than     782 bp
  95% of sequence in fragments shorter than     976 bp

# CviAII.MlyI (CATG+GAGTC) double digest, 17746 thousand fragments (13815697+3931692 sites), 167 bp average fragment length
   5% of sequence in fragments shorter than      56 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     112 bp
  20% of sequence in fragments shorter than     135 bp
  25% of sequence in fragments shorter than     159 bp
  30% of sequence in fragments shorter than     182 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     233 bp
  45% of sequence in fragments shorter than     260 bp
  50% of sequence in fragments shorter than     288 bp
  55% of sequence in fragments shorter than     316 bp
  60% of sequence in fragments shorter than     349 bp
  65% of sequence in fragments shorter than     387 bp
  70% of sequence in fragments shorter than     430 bp
  75% of sequence in fragments shorter than     480 bp
  80% of sequence in fragments shorter than     543 bp
  85% of sequence in fragments shorter than     622 bp
  90% of sequence in fragments shorter than     736 bp
  95% of sequence in fragments shorter than     949 bp

# Hpy188I.TspRI (TCNGA+CASTG) double digest, 17706 thousand fragments (8942153+8765240 sites), 168 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      88 bp
  15% of sequence in fragments shorter than     115 bp
  20% of sequence in fragments shorter than     139 bp
  25% of sequence in fragments shorter than     162 bp
  30% of sequence in fragments shorter than     183 bp
  35% of sequence in fragments shorter than     208 bp
  40% of sequence in fragments shorter than     229 bp
  45% of sequence in fragments shorter than     254 bp
  50% of sequence in fragments shorter than     281 bp
  55% of sequence in fragments shorter than     309 bp
  60% of sequence in fragments shorter than     341 bp
  65% of sequence in fragments shorter than     372 bp
  70% of sequence in fragments shorter than     411 bp
  75% of sequence in fragments shorter than     457 bp
  80% of sequence in fragments shorter than     513 bp
  85% of sequence in fragments shorter than     585 bp
  90% of sequence in fragments shorter than     685 bp
  95% of sequence in fragments shorter than     852 bp

# HinfI.TspRI (GANTC+CASTG) double digest, 17680 thousand fragments (8916054+8765240 sites), 168 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      88 bp
  15% of sequence in fragments shorter than     118 bp
  20% of sequence in fragments shorter than     144 bp
  25% of sequence in fragments shorter than     170 bp
  30% of sequence in fragments shorter than     192 bp
  35% of sequence in fragments shorter than     217 bp
  40% of sequence in fragments shorter than     243 bp
  45% of sequence in fragments shorter than     269 bp
  50% of sequence in fragments shorter than     299 bp
  55% of sequence in fragments shorter than     329 bp
  60% of sequence in fragments shorter than     359 bp
  65% of sequence in fragments shorter than     396 bp
  70% of sequence in fragments shorter than     439 bp
  75% of sequence in fragments shorter than     490 bp
  80% of sequence in fragments shorter than     551 bp
  85% of sequence in fragments shorter than     629 bp
  90% of sequence in fragments shorter than     732 bp
  95% of sequence in fragments shorter than     910 bp

# BsrI.ScrFI (ACTGG+CCNGG) double digest, 17545 thousand fragments (5741310+11805099 sites), 169 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      77 bp
  15% of sequence in fragments shorter than     105 bp
  20% of sequence in fragments shorter than     131 bp
  25% of sequence in fragments shorter than     160 bp
  30% of sequence in fragments shorter than     191 bp
  35% of sequence in fragments shorter than     224 bp
  40% of sequence in fragments shorter than     258 bp
  45% of sequence in fragments shorter than     296 bp
  50% of sequence in fragments shorter than     337 bp
  55% of sequence in fragments shorter than     383 bp
  60% of sequence in fragments shorter than     434 bp
  65% of sequence in fragments shorter than     490 bp
  70% of sequence in fragments shorter than     557 bp
  75% of sequence in fragments shorter than     637 bp
  80% of sequence in fragments shorter than     728 bp
  85% of sequence in fragments shorter than     857 bp
  90% of sequence in fragments shorter than    1044 bp
  95% of sequence in fragments shorter than    1401 bp

# HaeIII.Hpy188I (GGCC+TCNGA) double digest, 17503 thousand fragments (8562230+8942153 sites), 170 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      84 bp
  15% of sequence in fragments shorter than     112 bp
  20% of sequence in fragments shorter than     137 bp
  25% of sequence in fragments shorter than     165 bp
  30% of sequence in fragments shorter than     190 bp
  35% of sequence in fragments shorter than     219 bp
  40% of sequence in fragments shorter than     247 bp
  45% of sequence in fragments shorter than     278 bp
  50% of sequence in fragments shorter than     310 bp
  55% of sequence in fragments shorter than     343 bp
  60% of sequence in fragments shorter than     380 bp
  65% of sequence in fragments shorter than     420 bp
  70% of sequence in fragments shorter than     466 bp
  75% of sequence in fragments shorter than     522 bp
  80% of sequence in fragments shorter than     588 bp
  85% of sequence in fragments shorter than     675 bp
  90% of sequence in fragments shorter than     796 bp
  95% of sequence in fragments shorter than    1001 bp

# HaeIII.HinfI (GGCC+GANTC) double digest, 17477 thousand fragments (8562230+8916054 sites), 170 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      84 bp
  15% of sequence in fragments shorter than     109 bp
  20% of sequence in fragments shorter than     136 bp
  25% of sequence in fragments shorter than     161 bp
  30% of sequence in fragments shorter than     185 bp
  35% of sequence in fragments shorter than     212 bp
  40% of sequence in fragments shorter than     238 bp
  45% of sequence in fragments shorter than     270 bp
  50% of sequence in fragments shorter than     303 bp
  55% of sequence in fragments shorter than     339 bp
  60% of sequence in fragments shorter than     377 bp
  65% of sequence in fragments shorter than     421 bp
  70% of sequence in fragments shorter than     473 bp
  75% of sequence in fragments shorter than     533 bp
  80% of sequence in fragments shorter than     608 bp
  85% of sequence in fragments shorter than     702 bp
  90% of sequence in fragments shorter than     833 bp
  95% of sequence in fragments shorter than    1057 bp

# BscAI.CviAII (GCATC+CATG) double digest, 17468 thousand fragments (3652926+13815697 sites), 170 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      87 bp
  15% of sequence in fragments shorter than     114 bp
  20% of sequence in fragments shorter than     140 bp
  25% of sequence in fragments shorter than     164 bp
  30% of sequence in fragments shorter than     189 bp
  35% of sequence in fragments shorter than     215 bp
  40% of sequence in fragments shorter than     241 bp
  45% of sequence in fragments shorter than     269 bp
  50% of sequence in fragments shorter than     297 bp
  55% of sequence in fragments shorter than     327 bp
  60% of sequence in fragments shorter than     362 bp
  65% of sequence in fragments shorter than     400 bp
  70% of sequence in fragments shorter than     443 bp
  75% of sequence in fragments shorter than     494 bp
  80% of sequence in fragments shorter than     557 bp
  85% of sequence in fragments shorter than     636 bp
  90% of sequence in fragments shorter than     749 bp
  95% of sequence in fragments shorter than     952 bp

# DdeI.StyI (CTNAG+CCWWGG) double digest, 17425 thousand fragments (14312045+3114107 sites), 171 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      90 bp
  15% of sequence in fragments shorter than     116 bp
  20% of sequence in fragments shorter than     136 bp
  25% of sequence in fragments shorter than     159 bp
  30% of sequence in fragments shorter than     179 bp
  35% of sequence in fragments shorter than     205 bp
  40% of sequence in fragments shorter than     232 bp
  45% of sequence in fragments shorter than     260 bp
  50% of sequence in fragments shorter than     290 bp
  55% of sequence in fragments shorter than     322 bp
  60% of sequence in fragments shorter than     356 bp
  65% of sequence in fragments shorter than     396 bp
  70% of sequence in fragments shorter than     442 bp
  75% of sequence in fragments shorter than     496 bp
  80% of sequence in fragments shorter than     558 bp
  85% of sequence in fragments shorter than     639 bp
  90% of sequence in fragments shorter than     755 bp
  95% of sequence in fragments shorter than     961 bp

# DdeI.NspI (CTNAG+RCATGY) double digest, 17415 thousand fragments (14312045+3104359 sites), 171 bp average fragment length
   5% of sequence in fragments shorter than      58 bp
  10% of sequence in fragments shorter than      90 bp
  15% of sequence in fragments shorter than     116 bp
  20% of sequence in fragments shorter than     139 bp
  25% of sequence in fragments shorter than     164 bp
  30% of sequence in fragments shorter than     183 bp
  35% of sequence in fragments shorter than     207 bp
  40% of sequence in fragments shorter than     233 bp
  45% of sequence in fragments shorter than     260 bp
  50% of sequence in fragments shorter than     289 bp
  55% of sequence in fragments shorter than     319 bp
  60% of sequence in fragments shorter than     350 bp
  65% of sequence in fragments shorter than     388 bp
  70% of sequence in fragments shorter than     430 bp
  75% of sequence in fragments shorter than     479 bp
  80% of sequence in fragments shorter than     539 bp
  85% of sequence in fragments shorter than     614 bp
  90% of sequence in fragments shorter than     723 bp
  95% of sequence in fragments shorter than     903 bp

# CviAII.SfeI (CATG+CTRYAG) double digest, 17346 thousand fragments (13815697+3531009 sites), 171 bp average fragment length
   5% of sequence in fragments shorter than      58 bp
  10% of sequence in fragments shorter than      87 bp
  15% of sequence in fragments shorter than     115 bp
  20% of sequence in fragments shorter than     140 bp
  25% of sequence in fragments shorter than     164 bp
  30% of sequence in fragments shorter than     189 bp
  35% of sequence in fragments shorter than     215 bp
  40% of sequence in fragments shorter than     241 bp
  45% of sequence in fragments shorter than     268 bp
  50% of sequence in fragments shorter than     296 bp
  55% of sequence in fragments shorter than     326 bp
  60% of sequence in fragments shorter than     360 bp
  65% of sequence in fragments shorter than     398 bp
  70% of sequence in fragments shorter than     440 bp
  75% of sequence in fragments shorter than     490 bp
  80% of sequence in fragments shorter than     551 bp
  85% of sequence in fragments shorter than     629 bp
  90% of sequence in fragments shorter than     735 bp
  95% of sequence in fragments shorter than     939 bp

# HaeIII.TspRI (GGCC+CASTG) double digest, 17326 thousand fragments (8562230+8765240 sites), 171 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      87 bp
  15% of sequence in fragments shorter than     118 bp
  20% of sequence in fragments shorter than     142 bp
  25% of sequence in fragments shorter than     170 bp
  30% of sequence in fragments shorter than     194 bp
  35% of sequence in fragments shorter than     223 bp
  40% of sequence in fragments shorter than     255 bp
  45% of sequence in fragments shorter than     291 bp
  50% of sequence in fragments shorter than     330 bp
  55% of sequence in fragments shorter than     364 bp
  60% of sequence in fragments shorter than     411 bp
  65% of sequence in fragments shorter than     462 bp
  70% of sequence in fragments shorter than     521 bp
  75% of sequence in fragments shorter than     590 bp
  80% of sequence in fragments shorter than     673 bp
  85% of sequence in fragments shorter than     779 bp
  90% of sequence in fragments shorter than     922 bp
  95% of sequence in fragments shorter than    1177 bp

# AciI.AluI (CCGC+AGCT) double digest, 17233 thousand fragments (4206130+13027778 sites), 172 bp average fragment length
   5% of sequence in fragments shorter than      55 bp
  10% of sequence in fragments shorter than      88 bp
  15% of sequence in fragments shorter than     117 bp
  20% of sequence in fragments shorter than     145 bp
  25% of sequence in fragments shorter than     173 bp
  30% of sequence in fragments shorter than     202 bp
  35% of sequence in fragments shorter than     229 bp
  40% of sequence in fragments shorter than     259 bp
  45% of sequence in fragments shorter than     291 bp
  50% of sequence in fragments shorter than     324 bp
  55% of sequence in fragments shorter than     357 bp
  60% of sequence in fragments shorter than     397 bp
  65% of sequence in fragments shorter than     441 bp
  70% of sequence in fragments shorter than     490 bp
  75% of sequence in fragments shorter than     547 bp
  80% of sequence in fragments shorter than     617 bp
  85% of sequence in fragments shorter than     705 bp
  90% of sequence in fragments shorter than     831 bp
  95% of sequence in fragments shorter than    1044 bp

# BspNCI.BstNI (CCAGA+CCWGG) double digest, 17137 thousand fragments (7282436+9855646 sites), 173 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      82 bp
  15% of sequence in fragments shorter than     110 bp
  20% of sequence in fragments shorter than     133 bp
  25% of sequence in fragments shorter than     162 bp
  30% of sequence in fragments shorter than     189 bp
  35% of sequence in fragments shorter than     221 bp
  40% of sequence in fragments shorter than     254 bp
  45% of sequence in fragments shorter than     290 bp
  50% of sequence in fragments shorter than     329 bp
  55% of sequence in fragments shorter than     369 bp
  60% of sequence in fragments shorter than     418 bp
  65% of sequence in fragments shorter than     473 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     610 bp
  80% of sequence in fragments shorter than     702 bp
  85% of sequence in fragments shorter than     822 bp
  90% of sequence in fragments shorter than     996 bp
  95% of sequence in fragments shorter than    1280 bp

# BbvI.ScrFI (GCAGC+CCNGG) double digest, 17094 thousand fragments (5290051+11805099 sites), 174 bp average fragment length
   5% of sequence in fragments shorter than      51 bp
  10% of sequence in fragments shorter than      81 bp
  15% of sequence in fragments shorter than     112 bp
  20% of sequence in fragments shorter than     140 bp
  25% of sequence in fragments shorter than     176 bp
  30% of sequence in fragments shorter than     214 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     299 bp
  45% of sequence in fragments shorter than     345 bp
  50% of sequence in fragments shorter than     399 bp
  55% of sequence in fragments shorter than     458 bp
  60% of sequence in fragments shorter than     524 bp
  65% of sequence in fragments shorter than     599 bp
  70% of sequence in fragments shorter than     683 bp
  75% of sequence in fragments shorter than     787 bp
  80% of sequence in fragments shorter than     912 bp
  85% of sequence in fragments shorter than    1083 bp
  90% of sequence in fragments shorter than    1308 bp
  95% of sequence in fragments shorter than    1725 bp

# AvaII.DdeI (GGWCC+CTNAG) double digest, 17059 thousand fragments (2747690+14312045 sites), 174 bp average fragment length
   5% of sequence in fragments shorter than      59 bp
  10% of sequence in fragments shorter than      93 bp
  15% of sequence in fragments shorter than     118 bp
  20% of sequence in fragments shorter than     141 bp
  25% of sequence in fragments shorter than     167 bp
  30% of sequence in fragments shorter than     188 bp
  35% of sequence in fragments shorter than     215 bp
  40% of sequence in fragments shorter than     244 bp
  45% of sequence in fragments shorter than     272 bp
  50% of sequence in fragments shorter than     302 bp
  55% of sequence in fragments shorter than     335 bp
  60% of sequence in fragments shorter than     371 bp
  65% of sequence in fragments shorter than     412 bp
  70% of sequence in fragments shorter than     459 bp
  75% of sequence in fragments shorter than     515 bp
  80% of sequence in fragments shorter than     580 bp
  85% of sequence in fragments shorter than     667 bp
  90% of sequence in fragments shorter than     778 bp
  95% of sequence in fragments shorter than     982 bp

# BspKT6I.BstNI (GATC+CCWGG) double digest, 17054 thousand fragments (7199434+9855646 sites), 174 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     116 bp
  20% of sequence in fragments shorter than     143 bp
  25% of sequence in fragments shorter than     169 bp
  30% of sequence in fragments shorter than     198 bp
  35% of sequence in fragments shorter than     231 bp
  40% of sequence in fragments shorter than     266 bp
  45% of sequence in fragments shorter than     303 bp
  50% of sequence in fragments shorter than     341 bp
  55% of sequence in fragments shorter than     384 bp
  60% of sequence in fragments shorter than     431 bp
  65% of sequence in fragments shorter than     483 bp
  70% of sequence in fragments shorter than     547 bp
  75% of sequence in fragments shorter than     621 bp
  80% of sequence in fragments shorter than     709 bp
  85% of sequence in fragments shorter than     828 bp
  90% of sequence in fragments shorter than     998 bp
  95% of sequence in fragments shorter than    1311 bp

# AluI.Tsp45I (AGCT+GTSAC) double digest, 17049 thousand fragments (13027778+4021761 sites), 174 bp average fragment length
   5% of sequence in fragments shorter than      58 bp
  10% of sequence in fragments shorter than      89 bp
  15% of sequence in fragments shorter than     115 bp
  20% of sequence in fragments shorter than     142 bp
  25% of sequence in fragments shorter than     168 bp
  30% of sequence in fragments shorter than     193 bp
  35% of sequence in fragments shorter than     218 bp
  40% of sequence in fragments shorter than     243 bp
  45% of sequence in fragments shorter than     270 bp
  50% of sequence in fragments shorter than     299 bp
  55% of sequence in fragments shorter than     330 bp
  60% of sequence in fragments shorter than     362 bp
  65% of sequence in fragments shorter than     400 bp
  70% of sequence in fragments shorter than     443 bp
  75% of sequence in fragments shorter than     493 bp
  80% of sequence in fragments shorter than     555 bp
  85% of sequence in fragments shorter than     634 bp
  90% of sequence in fragments shorter than     746 bp
  95% of sequence in fragments shorter than     950 bp

# DdeI.SmlI (CTNAG+CTYRAG) double digest, 17039 thousand fragments (14312045+2727867 sites), 174 bp average fragment length
   5% of sequence in fragments shorter than      60 bp
  10% of sequence in fragments shorter than      94 bp
  15% of sequence in fragments shorter than     118 bp
  20% of sequence in fragments shorter than     140 bp
  25% of sequence in fragments shorter than     166 bp
  30% of sequence in fragments shorter than     186 bp
  35% of sequence in fragments shorter than     212 bp
  40% of sequence in fragments shorter than     240 bp
  45% of sequence in fragments shorter than     268 bp
  50% of sequence in fragments shorter than     297 bp
  55% of sequence in fragments shorter than     329 bp
  60% of sequence in fragments shorter than     362 bp
  65% of sequence in fragments shorter than     402 bp
  70% of sequence in fragments shorter than     447 bp
  75% of sequence in fragments shorter than     500 bp
  80% of sequence in fragments shorter than     564 bp
  85% of sequence in fragments shorter than     643 bp
  90% of sequence in fragments shorter than     757 bp
  95% of sequence in fragments shorter than     955 bp

# FokI.ScrFI (GGATG+CCNGG) double digest, 17005 thousand fragments (5201116+11805099 sites), 175 bp average fragment length
   5% of sequence in fragments shorter than      52 bp
  10% of sequence in fragments shorter than      82 bp
  15% of sequence in fragments shorter than     115 bp
  20% of sequence in fragments shorter than     140 bp
  25% of sequence in fragments shorter than     173 bp
  30% of sequence in fragments shorter than     208 bp
  35% of sequence in fragments shorter than     245 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     371 bp
  55% of sequence in fragments shorter than     422 bp
  60% of sequence in fragments shorter than     479 bp
  65% of sequence in fragments shorter than     545 bp
  70% of sequence in fragments shorter than     619 bp
  75% of sequence in fragments shorter than     709 bp
  80% of sequence in fragments shorter than     820 bp
  85% of sequence in fragments shorter than     960 bp
  90% of sequence in fragments shorter than    1167 bp
  95% of sequence in fragments shorter than    1543 bp

# AluI.MlyI (AGCT+GAGTC) double digest, 16958 thousand fragments (13027778+3931692 sites), 175 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      88 bp
  15% of sequence in fragments shorter than     113 bp
  20% of sequence in fragments shorter than     141 bp
  25% of sequence in fragments shorter than     168 bp
  30% of sequence in fragments shorter than     193 bp
  35% of sequence in fragments shorter than     219 bp
  40% of sequence in fragments shorter than     244 bp
  45% of sequence in fragments shorter than     272 bp
  50% of sequence in fragments shorter than     303 bp
  55% of sequence in fragments shorter than     335 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     408 bp
  70% of sequence in fragments shorter than     452 bp
  75% of sequence in fragments shorter than     504 bp
  80% of sequence in fragments shorter than     568 bp
  85% of sequence in fragments shorter than     651 bp
  90% of sequence in fragments shorter than     765 bp
  95% of sequence in fragments shorter than     976 bp

# CviAII.StyI (CATG+CCWWGG) double digest, 16929 thousand fragments (13815697+3114107 sites), 176 bp average fragment length
   5% of sequence in fragments shorter than      59 bp
  10% of sequence in fragments shorter than      92 bp
  15% of sequence in fragments shorter than     122 bp
  20% of sequence in fragments shorter than     149 bp
  25% of sequence in fragments shorter than     177 bp
  30% of sequence in fragments shorter than     205 bp
  35% of sequence in fragments shorter than     233 bp
  40% of sequence in fragments shorter than     262 bp
  45% of sequence in fragments shorter than     290 bp
  50% of sequence in fragments shorter than     320 bp
  55% of sequence in fragments shorter than     354 bp
  60% of sequence in fragments shorter than     392 bp
  65% of sequence in fragments shorter than     434 bp
  70% of sequence in fragments shorter than     482 bp
  75% of sequence in fragments shorter than     539 bp
  80% of sequence in fragments shorter than     607 bp
  85% of sequence in fragments shorter than     693 bp
  90% of sequence in fragments shorter than     823 bp
  95% of sequence in fragments shorter than    1049 bp

# CviQI.ScrFI (GTAC+CCNGG) double digest, 16919 thousand fragments (5115078+11805099 sites), 176 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      84 bp
  15% of sequence in fragments shorter than     113 bp
  20% of sequence in fragments shorter than     139 bp
  25% of sequence in fragments shorter than     169 bp
  30% of sequence in fragments shorter than     200 bp
  35% of sequence in fragments shorter than     233 bp
  40% of sequence in fragments shorter than     267 bp
  45% of sequence in fragments shorter than     304 bp
  50% of sequence in fragments shorter than     342 bp
  55% of sequence in fragments shorter than     385 bp
  60% of sequence in fragments shorter than     434 bp
  65% of sequence in fragments shorter than     486 bp
  70% of sequence in fragments shorter than     548 bp
  75% of sequence in fragments shorter than     619 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     825 bp
  90% of sequence in fragments shorter than     998 bp
  95% of sequence in fragments shorter than    1300 bp

# CviAII.NspI (CATG+RCATGY) double digest, 16919 thousand fragments (13815697+3104359 sites), 176 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     629 bp
  80% of sequence in fragments shorter than     707 bp
  85% of sequence in fragments shorter than     812 bp
  90% of sequence in fragments shorter than     957 bp
  95% of sequence in fragments shorter than    1231 bp

# BstNI.Hin4II (CCWGG+CCTTC) double digest, 16915 thousand fragments (9855646+7059915 sites), 176 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     115 bp
  20% of sequence in fragments shorter than     139 bp
  25% of sequence in fragments shorter than     168 bp
  30% of sequence in fragments shorter than     198 bp
  35% of sequence in fragments shorter than     230 bp
  40% of sequence in fragments shorter than     263 bp
  45% of sequence in fragments shorter than     300 bp
  50% of sequence in fragments shorter than     340 bp
  55% of sequence in fragments shorter than     381 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     549 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     722 bp
  85% of sequence in fragments shorter than     849 bp
  90% of sequence in fragments shorter than    1021 bp
  95% of sequence in fragments shorter than    1325 bp

# ScrFI.TfiI (CCNGG+GAWTC) double digest, 16788 thousand fragments (11805099+4984362 sites), 177 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      85 bp
  15% of sequence in fragments shorter than     114 bp
  20% of sequence in fragments shorter than     142 bp
  25% of sequence in fragments shorter than     174 bp
  30% of sequence in fragments shorter than     206 bp
  35% of sequence in fragments shorter than     242 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     350 bp
  55% of sequence in fragments shorter than     394 bp
  60% of sequence in fragments shorter than     444 bp
  65% of sequence in fragments shorter than     501 bp
  70% of sequence in fragments shorter than     564 bp
  75% of sequence in fragments shorter than     641 bp
  80% of sequence in fragments shorter than     729 bp
  85% of sequence in fragments shorter than     846 bp
  90% of sequence in fragments shorter than    1011 bp
  95% of sequence in fragments shorter than    1292 bp

# DdeI.SspI (CTNAG+AATATT) double digest, 16688 thousand fragments (14312045+2377267 sites), 178 bp average fragment length
   5% of sequence in fragments shorter than      62 bp
  10% of sequence in fragments shorter than      95 bp
  15% of sequence in fragments shorter than     120 bp
  20% of sequence in fragments shorter than     143 bp
  25% of sequence in fragments shorter than     168 bp
  30% of sequence in fragments shorter than     188 bp
  35% of sequence in fragments shorter than     213 bp
  40% of sequence in fragments shorter than     239 bp
  45% of sequence in fragments shorter than     267 bp
  50% of sequence in fragments shorter than     295 bp
  55% of sequence in fragments shorter than     325 bp
  60% of sequence in fragments shorter than     356 bp
  65% of sequence in fragments shorter than     393 bp
  70% of sequence in fragments shorter than     434 bp
  75% of sequence in fragments shorter than     483 bp
  80% of sequence in fragments shorter than     540 bp
  85% of sequence in fragments shorter than     615 bp
  90% of sequence in fragments shorter than     721 bp
  95% of sequence in fragments shorter than     903 bp

# AluI.BscAI (AGCT+GCATC) double digest, 16680 thousand fragments (13027778+3652926 sites), 178 bp average fragment length
   5% of sequence in fragments shorter than      60 bp
  10% of sequence in fragments shorter than      91 bp
  15% of sequence in fragments shorter than     119 bp
  20% of sequence in fragments shorter than     147 bp
  25% of sequence in fragments shorter than     173 bp
  30% of sequence in fragments shorter than     197 bp
  35% of sequence in fragments shorter than     222 bp
  40% of sequence in fragments shorter than     249 bp
  45% of sequence in fragments shorter than     276 bp
  50% of sequence in fragments shorter than     306 bp
  55% of sequence in fragments shorter than     337 bp
  60% of sequence in fragments shorter than     368 bp
  65% of sequence in fragments shorter than     407 bp
  70% of sequence in fragments shorter than     450 bp
  75% of sequence in fragments shorter than     501 bp
  80% of sequence in fragments shorter than     562 bp
  85% of sequence in fragments shorter than     643 bp
  90% of sequence in fragments shorter than     753 bp
  95% of sequence in fragments shorter than     957 bp

# DdeI.HpaII (CTNAG+CCGG) double digest, 16629 thousand fragments (14312045+2317722 sites), 179 bp average fragment length
   5% of sequence in fragments shorter than      58 bp
  10% of sequence in fragments shorter than      93 bp
  15% of sequence in fragments shorter than     120 bp
  20% of sequence in fragments shorter than     145 bp
  25% of sequence in fragments shorter than     172 bp
  30% of sequence in fragments shorter than     194 bp
  35% of sequence in fragments shorter than     223 bp
  40% of sequence in fragments shorter than     254 bp
  45% of sequence in fragments shorter than     285 bp
  50% of sequence in fragments shorter than     318 bp
  55% of sequence in fragments shorter than     352 bp
  60% of sequence in fragments shorter than     393 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     488 bp
  75% of sequence in fragments shorter than     546 bp
  80% of sequence in fragments shorter than     617 bp
  85% of sequence in fragments shorter than     711 bp
  90% of sequence in fragments shorter than     838 bp
  95% of sequence in fragments shorter than    1063 bp

# AvaII.CviAII (GGWCC+CATG) double digest, 16562 thousand fragments (2747690+13815697 sites), 179 bp average fragment length
   5% of sequence in fragments shorter than      60 bp
  10% of sequence in fragments shorter than      91 bp
  15% of sequence in fragments shorter than     121 bp
  20% of sequence in fragments shorter than     148 bp
  25% of sequence in fragments shorter than     174 bp
  30% of sequence in fragments shorter than     202 bp
  35% of sequence in fragments shorter than     229 bp
  40% of sequence in fragments shorter than     256 bp
  45% of sequence in fragments shorter than     285 bp
  50% of sequence in fragments shorter than     313 bp
  55% of sequence in fragments shorter than     345 bp
  60% of sequence in fragments shorter than     381 bp
  65% of sequence in fragments shorter than     421 bp
  70% of sequence in fragments shorter than     467 bp
  75% of sequence in fragments shorter than     521 bp
  80% of sequence in fragments shorter than     586 bp
  85% of sequence in fragments shorter than     669 bp
  90% of sequence in fragments shorter than     781 bp
  95% of sequence in fragments shorter than     994 bp

# AluI.SfeI (AGCT+CTRYAG) double digest, 16558 thousand fragments (13027778+3531009 sites), 179 bp average fragment length
   5% of sequence in fragments shorter than      60 bp
  10% of sequence in fragments shorter than      95 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     156 bp
  25% of sequence in fragments shorter than     182 bp
  30% of sequence in fragments shorter than     209 bp
  35% of sequence in fragments shorter than     235 bp
  40% of sequence in fragments shorter than     263 bp
  45% of sequence in fragments shorter than     292 bp
  50% of sequence in fragments shorter than     322 bp
  55% of sequence in fragments shorter than     353 bp
  60% of sequence in fragments shorter than     389 bp
  65% of sequence in fragments shorter than     430 bp
  70% of sequence in fragments shorter than     474 bp
  75% of sequence in fragments shorter than     528 bp
  80% of sequence in fragments shorter than     593 bp
  85% of sequence in fragments shorter than     676 bp
  90% of sequence in fragments shorter than     793 bp
  95% of sequence in fragments shorter than    1012 bp

# CviAII.SmlI (CATG+CTYRAG) double digest, 16543 thousand fragments (13815697+2727867 sites), 180 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      90 bp
  15% of sequence in fragments shorter than     119 bp
  20% of sequence in fragments shorter than     146 bp
  25% of sequence in fragments shorter than     173 bp
  30% of sequence in fragments shorter than     200 bp
  35% of sequence in fragments shorter than     227 bp
  40% of sequence in fragments shorter than     254 bp
  45% of sequence in fragments shorter than     283 bp
  50% of sequence in fragments shorter than     312 bp
  55% of sequence in fragments shorter than     344 bp
  60% of sequence in fragments shorter than     380 bp
  65% of sequence in fragments shorter than     420 bp
  70% of sequence in fragments shorter than     466 bp
  75% of sequence in fragments shorter than     520 bp
  80% of sequence in fragments shorter than     585 bp
  85% of sequence in fragments shorter than     669 bp
  90% of sequence in fragments shorter than     791 bp
  95% of sequence in fragments shorter than    1012 bp

# BsmAI.BstNI (GTCTC+CCWGG) double digest, 16486 thousand fragments (6631585+9855646 sites), 180 bp average fragment length
   5% of sequence in fragments shorter than      52 bp
  10% of sequence in fragments shorter than      86 bp
  15% of sequence in fragments shorter than     118 bp
  20% of sequence in fragments shorter than     147 bp
  25% of sequence in fragments shorter than     180 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     256 bp
  40% of sequence in fragments shorter than     299 bp
  45% of sequence in fragments shorter than     343 bp
  50% of sequence in fragments shorter than     392 bp
  55% of sequence in fragments shorter than     448 bp
  60% of sequence in fragments shorter than     510 bp
  65% of sequence in fragments shorter than     581 bp
  70% of sequence in fragments shorter than     664 bp
  75% of sequence in fragments shorter than     762 bp
  80% of sequence in fragments shorter than     889 bp
  85% of sequence in fragments shorter than    1057 bp
  90% of sequence in fragments shorter than    1312 bp
  95% of sequence in fragments shorter than    1807 bp

# BspNCI.MboII (CCAGA+GAAGA) double digest, 16481 thousand fragments (7282436+9199496 sites), 180 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      94 bp
  15% of sequence in fragments shorter than     123 bp
  20% of sequence in fragments shorter than     151 bp
  25% of sequence in fragments shorter than     175 bp
  30% of sequence in fragments shorter than     203 bp
  35% of sequence in fragments shorter than     231 bp
  40% of sequence in fragments shorter than     261 bp
  45% of sequence in fragments shorter than     292 bp
  50% of sequence in fragments shorter than     325 bp
  55% of sequence in fragments shorter than     361 bp
  60% of sequence in fragments shorter than     401 bp
  65% of sequence in fragments shorter than     444 bp
  70% of sequence in fragments shorter than     494 bp
  75% of sequence in fragments shorter than     552 bp
  80% of sequence in fragments shorter than     619 bp
  85% of sequence in fragments shorter than     706 bp
  90% of sequence in fragments shorter than     831 bp
  95% of sequence in fragments shorter than    1039 bp

# BspKT6I.MboII (GATC+GAAGA) double digest, 16398 thousand fragments (7199434+9199496 sites), 181 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     128 bp
  20% of sequence in fragments shorter than     153 bp
  25% of sequence in fragments shorter than     175 bp
  30% of sequence in fragments shorter than     195 bp
  35% of sequence in fragments shorter than     223 bp
  40% of sequence in fragments shorter than     250 bp
  45% of sequence in fragments shorter than     279 bp
  50% of sequence in fragments shorter than     308 bp
  55% of sequence in fragments shorter than     340 bp
  60% of sequence in fragments shorter than     372 bp
  65% of sequence in fragments shorter than     411 bp
  70% of sequence in fragments shorter than     456 bp
  75% of sequence in fragments shorter than     506 bp
  80% of sequence in fragments shorter than     568 bp
  85% of sequence in fragments shorter than     646 bp
  90% of sequence in fragments shorter than     749 bp
  95% of sequence in fragments shorter than     931 bp

# Hin4II.MboII (CCTTC+GAAGA) double digest, 16258 thousand fragments (7059915+9199496 sites), 183 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      97 bp
  15% of sequence in fragments shorter than     129 bp
  20% of sequence in fragments shorter than     157 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     312 bp
  50% of sequence in fragments shorter than     347 bp
  55% of sequence in fragments shorter than     385 bp
  60% of sequence in fragments shorter than     429 bp
  65% of sequence in fragments shorter than     477 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     593 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     763 bp
  90% of sequence in fragments shorter than     898 bp
  95% of sequence in fragments shorter than    1130 bp

# ApoI.BstNI (RAATTY+CCWGG) double digest, 16237 thousand fragments (6382433+9855646 sites), 183 bp average fragment length
   5% of sequence in fragments shorter than      59 bp
  10% of sequence in fragments shorter than      90 bp
  15% of sequence in fragments shorter than     118 bp
  20% of sequence in fragments shorter than     142 bp
  25% of sequence in fragments shorter than     169 bp
  30% of sequence in fragments shorter than     195 bp
  35% of sequence in fragments shorter than     222 bp
  40% of sequence in fragments shorter than     252 bp
  45% of sequence in fragments shorter than     281 bp
  50% of sequence in fragments shorter than     315 bp
  55% of sequence in fragments shorter than     350 bp
  60% of sequence in fragments shorter than     390 bp
  65% of sequence in fragments shorter than     433 bp
  70% of sequence in fragments shorter than     483 bp
  75% of sequence in fragments shorter than     541 bp
  80% of sequence in fragments shorter than     614 bp
  85% of sequence in fragments shorter than     705 bp
  90% of sequence in fragments shorter than     843 bp
  95% of sequence in fragments shorter than    1087 bp

# BspNCI.Hpy188I (CCAGA+TCNGA) double digest, 16224 thousand fragments (7282436+8942153 sites), 183 bp average fragment length
   5% of sequence in fragments shorter than      60 bp
  10% of sequence in fragments shorter than      92 bp
  15% of sequence in fragments shorter than     120 bp
  20% of sequence in fragments shorter than     148 bp
  25% of sequence in fragments shorter than     173 bp
  30% of sequence in fragments shorter than     198 bp
  35% of sequence in fragments shorter than     227 bp
  40% of sequence in fragments shorter than     257 bp
  45% of sequence in fragments shorter than     289 bp
  50% of sequence in fragments shorter than     323 bp
  55% of sequence in fragments shorter than     356 bp
  60% of sequence in fragments shorter than     396 bp
  65% of sequence in fragments shorter than     440 bp
  70% of sequence in fragments shorter than     489 bp
  75% of sequence in fragments shorter than     547 bp
  80% of sequence in fragments shorter than     613 bp
  85% of sequence in fragments shorter than     700 bp
  90% of sequence in fragments shorter than     820 bp
  95% of sequence in fragments shorter than    1028 bp

# BspNCI.HinfI (CCAGA+GANTC) double digest, 16197 thousand fragments (7282436+8916054 sites), 183 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     127 bp
  20% of sequence in fragments shorter than     155 bp
  25% of sequence in fragments shorter than     181 bp
  30% of sequence in fragments shorter than     209 bp
  35% of sequence in fragments shorter than     238 bp
  40% of sequence in fragments shorter than     267 bp
  45% of sequence in fragments shorter than     297 bp
  50% of sequence in fragments shorter than     327 bp
  55% of sequence in fragments shorter than     355 bp
  60% of sequence in fragments shorter than     391 bp
  65% of sequence in fragments shorter than     432 bp
  70% of sequence in fragments shorter than     478 bp
  75% of sequence in fragments shorter than     531 bp
  80% of sequence in fragments shorter than     597 bp
  85% of sequence in fragments shorter than     680 bp
  90% of sequence in fragments shorter than     792 bp
  95% of sequence in fragments shorter than     987 bp

# CviAII.SspI (CATG+AATATT) double digest, 16192 thousand fragments (13815697+2377267 sites), 184 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      93 bp
  15% of sequence in fragments shorter than     123 bp
  20% of sequence in fragments shorter than     149 bp
  25% of sequence in fragments shorter than     175 bp
  30% of sequence in fragments shorter than     203 bp
  35% of sequence in fragments shorter than     230 bp
  40% of sequence in fragments shorter than     257 bp
  45% of sequence in fragments shorter than     285 bp
  50% of sequence in fragments shorter than     314 bp
  55% of sequence in fragments shorter than     346 bp
  60% of sequence in fragments shorter than     382 bp
  65% of sequence in fragments shorter than     423 bp
  70% of sequence in fragments shorter than     469 bp
  75% of sequence in fragments shorter than     523 bp
  80% of sequence in fragments shorter than     589 bp
  85% of sequence in fragments shorter than     675 bp
  90% of sequence in fragments shorter than     799 bp
  95% of sequence in fragments shorter than    1034 bp

# AluI.StyI (AGCT+CCWWGG) double digest, 16141 thousand fragments (13027778+3114107 sites), 184 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      93 bp
  15% of sequence in fragments shorter than     122 bp
  20% of sequence in fragments shorter than     149 bp
  25% of sequence in fragments shorter than     176 bp
  30% of sequence in fragments shorter than     202 bp
  35% of sequence in fragments shorter than     229 bp
  40% of sequence in fragments shorter than     257 bp
  45% of sequence in fragments shorter than     286 bp
  50% of sequence in fragments shorter than     317 bp
  55% of sequence in fragments shorter than     349 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     428 bp
  70% of sequence in fragments shorter than     474 bp
  75% of sequence in fragments shorter than     529 bp
  80% of sequence in fragments shorter than     596 bp
  85% of sequence in fragments shorter than     679 bp
  90% of sequence in fragments shorter than     797 bp
  95% of sequence in fragments shorter than    1016 bp

# BspKT6I.Hpy188I (GATC+TCNGA) double digest, 16141 thousand fragments (7199434+8942153 sites), 184 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     177 bp
  30% of sequence in fragments shorter than     199 bp
  35% of sequence in fragments shorter than     226 bp
  40% of sequence in fragments shorter than     254 bp
  45% of sequence in fragments shorter than     284 bp
  50% of sequence in fragments shorter than     314 bp
  55% of sequence in fragments shorter than     346 bp
  60% of sequence in fragments shorter than     380 bp
  65% of sequence in fragments shorter than     419 bp
  70% of sequence in fragments shorter than     464 bp
  75% of sequence in fragments shorter than     517 bp
  80% of sequence in fragments shorter than     579 bp
  85% of sequence in fragments shorter than     658 bp
  90% of sequence in fragments shorter than     767 bp
  95% of sequence in fragments shorter than     956 bp

# CviAII.HpaII (CATG+CCGG) double digest, 16132 thousand fragments (13815697+2317722 sites), 184 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      93 bp
  15% of sequence in fragments shorter than     123 bp
  20% of sequence in fragments shorter than     150 bp
  25% of sequence in fragments shorter than     178 bp
  30% of sequence in fragments shorter than     207 bp
  35% of sequence in fragments shorter than     236 bp
  40% of sequence in fragments shorter than     265 bp
  45% of sequence in fragments shorter than     295 bp
  50% of sequence in fragments shorter than     327 bp
  55% of sequence in fragments shorter than     362 bp
  60% of sequence in fragments shorter than     401 bp
  65% of sequence in fragments shorter than     445 bp
  70% of sequence in fragments shorter than     495 bp
  75% of sequence in fragments shorter than     554 bp
  80% of sequence in fragments shorter than     623 bp
  85% of sequence in fragments shorter than     712 bp
  90% of sequence in fragments shorter than     842 bp
  95% of sequence in fragments shorter than    1074 bp

# AluI.NspI (AGCT+RCATGY) double digest, 16131 thousand fragments (13027778+3104359 sites), 184 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     155 bp
  25% of sequence in fragments shorter than     180 bp
  30% of sequence in fragments shorter than     205 bp
  35% of sequence in fragments shorter than     230 bp
  40% of sequence in fragments shorter than     256 bp
  45% of sequence in fragments shorter than     284 bp
  50% of sequence in fragments shorter than     313 bp
  55% of sequence in fragments shorter than     343 bp
  60% of sequence in fragments shorter than     377 bp
  65% of sequence in fragments shorter than     415 bp
  70% of sequence in fragments shorter than     459 bp
  75% of sequence in fragments shorter than     509 bp
  80% of sequence in fragments shorter than     567 bp
  85% of sequence in fragments shorter than     645 bp
  90% of sequence in fragments shorter than     754 bp
  95% of sequence in fragments shorter than     950 bp

# BspKT6I.HinfI (GATC+GANTC) double digest, 16114 thousand fragments (7199434+8916054 sites), 184 bp average fragment length
   5% of sequence in fragments shorter than      62 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     125 bp
  20% of sequence in fragments shorter than     146 bp
  25% of sequence in fragments shorter than     172 bp
  30% of sequence in fragments shorter than     195 bp
  35% of sequence in fragments shorter than     220 bp
  40% of sequence in fragments shorter than     249 bp
  45% of sequence in fragments shorter than     278 bp
  50% of sequence in fragments shorter than     309 bp
  55% of sequence in fragments shorter than     341 bp
  60% of sequence in fragments shorter than     373 bp
  65% of sequence in fragments shorter than     414 bp
  70% of sequence in fragments shorter than     459 bp
  75% of sequence in fragments shorter than     513 bp
  80% of sequence in fragments shorter than     578 bp
  85% of sequence in fragments shorter than     656 bp
  90% of sequence in fragments shorter than     763 bp
  95% of sequence in fragments shorter than     949 bp

# DdeI.HhaI (CTNAG+GCGC) double digest, 16068 thousand fragments (14312045+1756502 sites), 185 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      97 bp
  15% of sequence in fragments shorter than     123 bp
  20% of sequence in fragments shorter than     150 bp
  25% of sequence in fragments shorter than     174 bp
  30% of sequence in fragments shorter than     202 bp
  35% of sequence in fragments shorter than     232 bp
  40% of sequence in fragments shorter than     263 bp
  45% of sequence in fragments shorter than     294 bp
  50% of sequence in fragments shorter than     328 bp
  55% of sequence in fragments shorter than     364 bp
  60% of sequence in fragments shorter than     404 bp
  65% of sequence in fragments shorter than     449 bp
  70% of sequence in fragments shorter than     502 bp
  75% of sequence in fragments shorter than     561 bp
  80% of sequence in fragments shorter than     634 bp
  85% of sequence in fragments shorter than     728 bp
  90% of sequence in fragments shorter than     859 bp
  95% of sequence in fragments shorter than    1088 bp

# BstNI.BthCI (CCWGG+GCNGC) double digest, 16065 thousand fragments (9855646+6209929 sites), 185 bp average fragment length
   5% of sequence in fragments shorter than      54 bp
  10% of sequence in fragments shorter than      87 bp
  15% of sequence in fragments shorter than     119 bp
  20% of sequence in fragments shorter than     153 bp
  25% of sequence in fragments shorter than     187 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     266 bp
  40% of sequence in fragments shorter than     310 bp
  45% of sequence in fragments shorter than     356 bp
  50% of sequence in fragments shorter than     410 bp
  55% of sequence in fragments shorter than     468 bp
  60% of sequence in fragments shorter than     535 bp
  65% of sequence in fragments shorter than     607 bp
  70% of sequence in fragments shorter than     690 bp
  75% of sequence in fragments shorter than     792 bp
  80% of sequence in fragments shorter than     915 bp
  85% of sequence in fragments shorter than    1081 bp
  90% of sequence in fragments shorter than    1305 bp
  95% of sequence in fragments shorter than    1706 bp

# BspNCI.TspRI (CCAGA+CASTG) double digest, 16047 thousand fragments (7282436+8765240 sites), 185 bp average fragment length
   5% of sequence in fragments shorter than      62 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     127 bp
  20% of sequence in fragments shorter than     154 bp
  25% of sequence in fragments shorter than     181 bp
  30% of sequence in fragments shorter than     209 bp
  35% of sequence in fragments shorter than     233 bp
  40% of sequence in fragments shorter than     261 bp
  45% of sequence in fragments shorter than     292 bp
  50% of sequence in fragments shorter than     325 bp
  55% of sequence in fragments shorter than     357 bp
  60% of sequence in fragments shorter than     396 bp
  65% of sequence in fragments shorter than     438 bp
  70% of sequence in fragments shorter than     487 bp
  75% of sequence in fragments shorter than     545 bp
  80% of sequence in fragments shorter than     613 bp
  85% of sequence in fragments shorter than     701 bp
  90% of sequence in fragments shorter than     824 bp
  95% of sequence in fragments shorter than    1040 bp

# BccI.BstNI (CCATC+CCWGG) double digest, 16026 thousand fragments (6170927+9855646 sites), 185 bp average fragment length
   5% of sequence in fragments shorter than      56 bp
  10% of sequence in fragments shorter than      89 bp
  15% of sequence in fragments shorter than     119 bp
  20% of sequence in fragments shorter than     147 bp
  25% of sequence in fragments shorter than     177 bp
  30% of sequence in fragments shorter than     209 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     283 bp
  45% of sequence in fragments shorter than     323 bp
  50% of sequence in fragments shorter than     366 bp
  55% of sequence in fragments shorter than     414 bp
  60% of sequence in fragments shorter than     468 bp
  65% of sequence in fragments shorter than     531 bp
  70% of sequence in fragments shorter than     602 bp
  75% of sequence in fragments shorter than     688 bp
  80% of sequence in fragments shorter than     796 bp
  85% of sequence in fragments shorter than     938 bp
  90% of sequence in fragments shorter than    1150 bp
  95% of sequence in fragments shorter than    1551 bp

# BfiI.DdeI (ACTGGG+CTNAG) double digest, 16018 thousand fragments (1706594+14312045 sites), 186 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     124 bp
  20% of sequence in fragments shorter than     150 bp
  25% of sequence in fragments shorter than     173 bp
  30% of sequence in fragments shorter than     199 bp
  35% of sequence in fragments shorter than     227 bp
  40% of sequence in fragments shorter than     255 bp
  45% of sequence in fragments shorter than     285 bp
  50% of sequence in fragments shorter than     317 bp
  55% of sequence in fragments shorter than     350 bp
  60% of sequence in fragments shorter than     389 bp
  65% of sequence in fragments shorter than     430 bp
  70% of sequence in fragments shorter than     479 bp
  75% of sequence in fragments shorter than     536 bp
  80% of sequence in fragments shorter than     603 bp
  85% of sequence in fragments shorter than     692 bp
  90% of sequence in fragments shorter than     813 bp
  95% of sequence in fragments shorter than    1027 bp

# AciI.ScrFI (CCGC+CCNGG) double digest, 16010 thousand fragments (4206130+11805099 sites), 186 bp average fragment length
   5% of sequence in fragments shorter than      50 bp
  10% of sequence in fragments shorter than      83 bp
  15% of sequence in fragments shorter than     120 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     207 bp
  30% of sequence in fragments shorter than     258 bp
  35% of sequence in fragments shorter than     312 bp
  40% of sequence in fragments shorter than     368 bp
  45% of sequence in fragments shorter than     432 bp
  50% of sequence in fragments shorter than     503 bp
  55% of sequence in fragments shorter than     580 bp
  60% of sequence in fragments shorter than     666 bp
  65% of sequence in fragments shorter than     763 bp
  70% of sequence in fragments shorter than     878 bp
  75% of sequence in fragments shorter than    1016 bp
  80% of sequence in fragments shorter than    1185 bp
  85% of sequence in fragments shorter than    1399 bp
  90% of sequence in fragments shorter than    1718 bp
  95% of sequence in fragments shorter than    2262 bp

# Hin4II.Hpy188I (CCTTC+TCNGA) double digest, 16001 thousand fragments (7059915+8942153 sites), 186 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      97 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     155 bp
  25% of sequence in fragments shorter than     181 bp
  30% of sequence in fragments shorter than     211 bp
  35% of sequence in fragments shorter than     239 bp
  40% of sequence in fragments shorter than     272 bp
  45% of sequence in fragments shorter than     306 bp
  50% of sequence in fragments shorter than     342 bp
  55% of sequence in fragments shorter than     379 bp
  60% of sequence in fragments shorter than     420 bp
  65% of sequence in fragments shorter than     468 bp
  70% of sequence in fragments shorter than     522 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     658 bp
  85% of sequence in fragments shorter than     755 bp
  90% of sequence in fragments shorter than     889 bp
  95% of sequence in fragments shorter than    1119 bp

# Hin4II.HinfI (CCTTC+GANTC) double digest, 15975 thousand fragments (7059915+8916054 sites), 186 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than      95 bp
  15% of sequence in fragments shorter than     127 bp
  20% of sequence in fragments shorter than     154 bp
  25% of sequence in fragments shorter than     182 bp
  30% of sequence in fragments shorter than     210 bp
  35% of sequence in fragments shorter than     237 bp
  40% of sequence in fragments shorter than     266 bp
  45% of sequence in fragments shorter than     297 bp
  50% of sequence in fragments shorter than     326 bp
  55% of sequence in fragments shorter than     358 bp
  60% of sequence in fragments shorter than     394 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     483 bp
  75% of sequence in fragments shorter than     538 bp
  80% of sequence in fragments shorter than     605 bp
  85% of sequence in fragments shorter than     689 bp
  90% of sequence in fragments shorter than     807 bp
  95% of sequence in fragments shorter than    1009 bp

# BspKT6I.TspRI (GATC+CASTG) double digest, 15964 thousand fragments (7199434+8765240 sites), 186 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     178 bp
  30% of sequence in fragments shorter than     205 bp
  35% of sequence in fragments shorter than     234 bp
  40% of sequence in fragments shorter than     264 bp
  45% of sequence in fragments shorter than     296 bp
  50% of sequence in fragments shorter than     330 bp
  55% of sequence in fragments shorter than     365 bp
  60% of sequence in fragments shorter than     404 bp
  65% of sequence in fragments shorter than     449 bp
  70% of sequence in fragments shorter than     499 bp
  75% of sequence in fragments shorter than     558 bp
  80% of sequence in fragments shorter than     630 bp
  85% of sequence in fragments shorter than     717 bp
  90% of sequence in fragments shorter than     851 bp
  95% of sequence in fragments shorter than    1073 bp

# DdeI.PsiI (CTNAG+TTATAA) double digest, 15959 thousand fragments (14312045+1647912 sites), 186 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     150 bp
  25% of sequence in fragments shorter than     173 bp
  30% of sequence in fragments shorter than     198 bp
  35% of sequence in fragments shorter than     224 bp
  40% of sequence in fragments shorter than     252 bp
  45% of sequence in fragments shorter than     281 bp
  50% of sequence in fragments shorter than     311 bp
  55% of sequence in fragments shorter than     343 bp
  60% of sequence in fragments shorter than     377 bp
  65% of sequence in fragments shorter than     416 bp
  70% of sequence in fragments shorter than     461 bp
  75% of sequence in fragments shorter than     514 bp
  80% of sequence in fragments shorter than     574 bp
  85% of sequence in fragments shorter than     654 bp
  90% of sequence in fragments shorter than     766 bp
  95% of sequence in fragments shorter than     958 bp

# DdeI.EsaBC3I (CTNAG+TCGA) double digest, 15914 thousand fragments (14312045+1603346 sites), 187 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     123 bp
  20% of sequence in fragments shorter than     150 bp
  25% of sequence in fragments shorter than     174 bp
  30% of sequence in fragments shorter than     200 bp
  35% of sequence in fragments shorter than     229 bp
  40% of sequence in fragments shorter than     259 bp
  45% of sequence in fragments shorter than     289 bp
  50% of sequence in fragments shorter than     320 bp
  55% of sequence in fragments shorter than     354 bp
  60% of sequence in fragments shorter than     393 bp
  65% of sequence in fragments shorter than     434 bp
  70% of sequence in fragments shorter than     482 bp
  75% of sequence in fragments shorter than     539 bp
  80% of sequence in fragments shorter than     606 bp
  85% of sequence in fragments shorter than     693 bp
  90% of sequence in fragments shorter than     813 bp
  95% of sequence in fragments shorter than    1022 bp

# BstNI.HphI (CCWGG+GGTGA) double digest, 15862 thousand fragments (9855646+6007336 sites), 187 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      91 bp
  15% of sequence in fragments shorter than     120 bp
  20% of sequence in fragments shorter than     153 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     220 bp
  35% of sequence in fragments shorter than     259 bp
  40% of sequence in fragments shorter than     298 bp
  45% of sequence in fragments shorter than     342 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     439 bp
  60% of sequence in fragments shorter than     497 bp
  65% of sequence in fragments shorter than     562 bp
  70% of sequence in fragments shorter than     638 bp
  75% of sequence in fragments shorter than     726 bp
  80% of sequence in fragments shorter than     841 bp
  85% of sequence in fragments shorter than     989 bp
  90% of sequence in fragments shorter than    1206 bp
  95% of sequence in fragments shorter than    1577 bp

# DdeI.HgiCI (CTNAG+GGYRCC) double digest, 15862 thousand fragments (14312045+1550877 sites), 187 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      98 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     153 bp
  25% of sequence in fragments shorter than     175 bp
  30% of sequence in fragments shorter than     204 bp
  35% of sequence in fragments shorter than     233 bp
  40% of sequence in fragments shorter than     263 bp
  45% of sequence in fragments shorter than     293 bp
  50% of sequence in fragments shorter than     327 bp
  55% of sequence in fragments shorter than     361 bp
  60% of sequence in fragments shorter than     400 bp
  65% of sequence in fragments shorter than     443 bp
  70% of sequence in fragments shorter than     492 bp
  75% of sequence in fragments shorter than     550 bp
  80% of sequence in fragments shorter than     621 bp
  85% of sequence in fragments shorter than     709 bp
  90% of sequence in fragments shorter than     829 bp
  95% of sequence in fragments shorter than    1051 bp

# BsmI.DdeI (GAATGC+CTNAG) double digest, 15860 thousand fragments (1549350+14312045 sites), 187 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     128 bp
  20% of sequence in fragments shorter than     151 bp
  25% of sequence in fragments shorter than     175 bp
  30% of sequence in fragments shorter than     203 bp
  35% of sequence in fragments shorter than     232 bp
  40% of sequence in fragments shorter than     262 bp
  45% of sequence in fragments shorter than     292 bp
  50% of sequence in fragments shorter than     324 bp
  55% of sequence in fragments shorter than     357 bp
  60% of sequence in fragments shorter than     395 bp
  65% of sequence in fragments shorter than     438 bp
  70% of sequence in fragments shorter than     487 bp
  75% of sequence in fragments shorter than     544 bp
  80% of sequence in fragments shorter than     611 bp
  85% of sequence in fragments shorter than     699 bp
  90% of sequence in fragments shorter than     817 bp
  95% of sequence in fragments shorter than    1026 bp

# BspNCI.HaeIII (CCAGA+GGCC) double digest, 15844 thousand fragments (7282436+8562230 sites), 188 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      94 bp
  15% of sequence in fragments shorter than     129 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     195 bp
  30% of sequence in fragments shorter than     229 bp
  35% of sequence in fragments shorter than     265 bp
  40% of sequence in fragments shorter than     302 bp
  45% of sequence in fragments shorter than     339 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     420 bp
  60% of sequence in fragments shorter than     467 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     579 bp
  75% of sequence in fragments shorter than     651 bp
  80% of sequence in fragments shorter than     734 bp
  85% of sequence in fragments shorter than     848 bp
  90% of sequence in fragments shorter than    1009 bp
  95% of sequence in fragments shorter than    1289 bp

# BsmAI.MboII (GTCTC+GAAGA) double digest, 15830 thousand fragments (6631585+9199496 sites), 188 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     179 bp
  30% of sequence in fragments shorter than     204 bp
  35% of sequence in fragments shorter than     233 bp
  40% of sequence in fragments shorter than     263 bp
  45% of sequence in fragments shorter than     295 bp
  50% of sequence in fragments shorter than     326 bp
  55% of sequence in fragments shorter than     359 bp
  60% of sequence in fragments shorter than     397 bp
  65% of sequence in fragments shorter than     439 bp
  70% of sequence in fragments shorter than     487 bp
  75% of sequence in fragments shorter than     542 bp
  80% of sequence in fragments shorter than     608 bp
  85% of sequence in fragments shorter than     692 bp
  90% of sequence in fragments shorter than     812 bp
  95% of sequence in fragments shorter than    1012 bp

# ScrFI.Tsp45I (CCNGG+GTSAC) double digest, 15826 thousand fragments (11805099+4021761 sites), 188 bp average fragment length
   5% of sequence in fragments shorter than      54 bp
  10% of sequence in fragments shorter than      86 bp
  15% of sequence in fragments shorter than     118 bp
  20% of sequence in fragments shorter than     149 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     264 bp
  40% of sequence in fragments shorter than     308 bp
  45% of sequence in fragments shorter than     351 bp
  50% of sequence in fragments shorter than     402 bp
  55% of sequence in fragments shorter than     460 bp
  60% of sequence in fragments shorter than     525 bp
  65% of sequence in fragments shorter than     597 bp
  70% of sequence in fragments shorter than     679 bp
  75% of sequence in fragments shorter than     779 bp
  80% of sequence in fragments shorter than     905 bp
  85% of sequence in fragments shorter than    1071 bp
  90% of sequence in fragments shorter than    1321 bp
  95% of sequence in fragments shorter than    1800 bp

# Hin4II.TspRI (CCTTC+CASTG) double digest, 15824 thousand fragments (7059915+8765240 sites), 188 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     242 bp
  40% of sequence in fragments shorter than     272 bp
  45% of sequence in fragments shorter than     302 bp
  50% of sequence in fragments shorter than     335 bp
  55% of sequence in fragments shorter than     369 bp
  60% of sequence in fragments shorter than     407 bp
  65% of sequence in fragments shorter than     450 bp
  70% of sequence in fragments shorter than     499 bp
  75% of sequence in fragments shorter than     555 bp
  80% of sequence in fragments shorter than     627 bp
  85% of sequence in fragments shorter than     718 bp
  90% of sequence in fragments shorter than     850 bp
  95% of sequence in fragments shorter than    1065 bp

# AflIII.DdeI (ACRYGT+CTNAG) double digest, 15796 thousand fragments (1485394+14312045 sites), 188 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     127 bp
  20% of sequence in fragments shorter than     152 bp
  25% of sequence in fragments shorter than     174 bp
  30% of sequence in fragments shorter than     200 bp
  35% of sequence in fragments shorter than     227 bp
  40% of sequence in fragments shorter than     256 bp
  45% of sequence in fragments shorter than     285 bp
  50% of sequence in fragments shorter than     316 bp
  55% of sequence in fragments shorter than     348 bp
  60% of sequence in fragments shorter than     385 bp
  65% of sequence in fragments shorter than     426 bp
  70% of sequence in fragments shorter than     473 bp
  75% of sequence in fragments shorter than     528 bp
  80% of sequence in fragments shorter than     591 bp
  85% of sequence in fragments shorter than     676 bp
  90% of sequence in fragments shorter than     792 bp
  95% of sequence in fragments shorter than     999 bp

# AluI.AvaII (AGCT+GGWCC) double digest, 15774 thousand fragments (13027778+2747690 sites), 188 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     127 bp
  20% of sequence in fragments shorter than     157 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     210 bp
  35% of sequence in fragments shorter than     237 bp
  40% of sequence in fragments shorter than     265 bp
  45% of sequence in fragments shorter than     295 bp
  50% of sequence in fragments shorter than     326 bp
  55% of sequence in fragments shorter than     358 bp
  60% of sequence in fragments shorter than     396 bp
  65% of sequence in fragments shorter than     439 bp
  70% of sequence in fragments shorter than     486 bp
  75% of sequence in fragments shorter than     543 bp
  80% of sequence in fragments shorter than     612 bp
  85% of sequence in fragments shorter than     700 bp
  90% of sequence in fragments shorter than     824 bp
  95% of sequence in fragments shorter than    1049 bp

# BspKT6I.HaeIII (GATC+GGCC) double digest, 15761 thousand fragments (7199434+8562230 sites), 189 bp average fragment length
   5% of sequence in fragments shorter than      59 bp
  10% of sequence in fragments shorter than      97 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     182 bp
  30% of sequence in fragments shorter than     214 bp
  35% of sequence in fragments shorter than     248 bp
  40% of sequence in fragments shorter than     282 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     351 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     436 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     545 bp
  75% of sequence in fragments shorter than     615 bp
  80% of sequence in fragments shorter than     697 bp
  85% of sequence in fragments shorter than     804 bp
  90% of sequence in fragments shorter than     955 bp
  95% of sequence in fragments shorter than    1215 bp

# AluI.SmlI (AGCT+CTYRAG) double digest, 15755 thousand fragments (13027778+2727867 sites), 189 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     129 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     213 bp
  35% of sequence in fragments shorter than     239 bp
  40% of sequence in fragments shorter than     267 bp
  45% of sequence in fragments shorter than     297 bp
  50% of sequence in fragments shorter than     328 bp
  55% of sequence in fragments shorter than     360 bp
  60% of sequence in fragments shorter than     397 bp
  65% of sequence in fragments shorter than     439 bp
  70% of sequence in fragments shorter than     486 bp
  75% of sequence in fragments shorter than     542 bp
  80% of sequence in fragments shorter than     610 bp
  85% of sequence in fragments shorter than     696 bp
  90% of sequence in fragments shorter than     818 bp
  95% of sequence in fragments shorter than    1037 bp

# DdeI.FauI (CTNAG+CCCGC) double digest, 15751 thousand fragments (14312045+1439772 sites), 189 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     153 bp
  25% of sequence in fragments shorter than     175 bp
  30% of sequence in fragments shorter than     205 bp
  35% of sequence in fragments shorter than     235 bp
  40% of sequence in fragments shorter than     266 bp
  45% of sequence in fragments shorter than     297 bp
  50% of sequence in fragments shorter than     332 bp
  55% of sequence in fragments shorter than     367 bp
  60% of sequence in fragments shorter than     407 bp
  65% of sequence in fragments shorter than     452 bp
  70% of sequence in fragments shorter than     505 bp
  75% of sequence in fragments shorter than     565 bp
  80% of sequence in fragments shorter than     637 bp
  85% of sequence in fragments shorter than     732 bp
  90% of sequence in fragments shorter than     864 bp
  95% of sequence in fragments shorter than    1093 bp

# BspMI.DdeI (ACCTGC+CTNAG) double digest, 15749 thousand fragments (1438129+14312045 sites), 189 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     152 bp
  25% of sequence in fragments shorter than     175 bp
  30% of sequence in fragments shorter than     204 bp
  35% of sequence in fragments shorter than     232 bp
  40% of sequence in fragments shorter than     261 bp
  45% of sequence in fragments shorter than     291 bp
  50% of sequence in fragments shorter than     324 bp
  55% of sequence in fragments shorter than     358 bp
  60% of sequence in fragments shorter than     398 bp
  65% of sequence in fragments shorter than     440 bp
  70% of sequence in fragments shorter than     491 bp
  75% of sequence in fragments shorter than     549 bp
  80% of sequence in fragments shorter than     618 bp
  85% of sequence in fragments shorter than     710 bp
  90% of sequence in fragments shorter than     836 bp
  95% of sequence in fragments shorter than    1062 bp

# MlyI.ScrFI (GAGTC+CCNGG) double digest, 15736 thousand fragments (3931692+11805099 sites), 189 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      86 bp
  15% of sequence in fragments shorter than     118 bp
  20% of sequence in fragments shorter than     150 bp
  25% of sequence in fragments shorter than     188 bp
  30% of sequence in fragments shorter than     227 bp
  35% of sequence in fragments shorter than     270 bp
  40% of sequence in fragments shorter than     316 bp
  45% of sequence in fragments shorter than     364 bp
  50% of sequence in fragments shorter than     418 bp
  55% of sequence in fragments shorter than     479 bp
  60% of sequence in fragments shorter than     549 bp
  65% of sequence in fragments shorter than     625 bp
  70% of sequence in fragments shorter than     716 bp
  75% of sequence in fragments shorter than     824 bp
  80% of sequence in fragments shorter than     961 bp
  85% of sequence in fragments shorter than    1146 bp
  90% of sequence in fragments shorter than    1428 bp
  95% of sequence in fragments shorter than    1975 bp

# DdeI.PstI (CTNAG+CTGCAG) double digest, 15633 thousand fragments (14312045+1321469 sites), 190 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     127 bp
  20% of sequence in fragments shorter than     154 bp
  25% of sequence in fragments shorter than     176 bp
  30% of sequence in fragments shorter than     205 bp
  35% of sequence in fragments shorter than     234 bp
  40% of sequence in fragments shorter than     264 bp
  45% of sequence in fragments shorter than     295 bp
  50% of sequence in fragments shorter than     328 bp
  55% of sequence in fragments shorter than     361 bp
  60% of sequence in fragments shorter than     401 bp
  65% of sequence in fragments shorter than     444 bp
  70% of sequence in fragments shorter than     495 bp
  75% of sequence in fragments shorter than     554 bp
  80% of sequence in fragments shorter than     623 bp
  85% of sequence in fragments shorter than     716 bp
  90% of sequence in fragments shorter than     843 bp
  95% of sequence in fragments shorter than    1067 bp

# HaeIII.Hin4II (GGCC+CCTTC) double digest, 15621 thousand fragments (8562230+7059915 sites), 190 bp average fragment length
   5% of sequence in fragments shorter than      58 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     237 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     310 bp
  45% of sequence in fragments shorter than     346 bp
  50% of sequence in fragments shorter than     388 bp
  55% of sequence in fragments shorter than     432 bp
  60% of sequence in fragments shorter than     483 bp
  65% of sequence in fragments shorter than     537 bp
  70% of sequence in fragments shorter than     601 bp
  75% of sequence in fragments shorter than     679 bp
  80% of sequence in fragments shorter than     772 bp
  85% of sequence in fragments shorter than     895 bp
  90% of sequence in fragments shorter than    1063 bp
  95% of sequence in fragments shorter than    1361 bp

# BsrI.BstNI (ACTGG+CCWGG) double digest, 15596 thousand fragments (5741310+9855646 sites), 191 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      89 bp
  15% of sequence in fragments shorter than     119 bp
  20% of sequence in fragments shorter than     148 bp
  25% of sequence in fragments shorter than     176 bp
  30% of sequence in fragments shorter than     208 bp
  35% of sequence in fragments shorter than     242 bp
  40% of sequence in fragments shorter than     277 bp
  45% of sequence in fragments shorter than     315 bp
  50% of sequence in fragments shorter than     357 bp
  55% of sequence in fragments shorter than     404 bp
  60% of sequence in fragments shorter than     455 bp
  65% of sequence in fragments shorter than     512 bp
  70% of sequence in fragments shorter than     580 bp
  75% of sequence in fragments shorter than     657 bp
  80% of sequence in fragments shorter than     752 bp
  85% of sequence in fragments shorter than     882 bp
  90% of sequence in fragments shorter than    1073 bp
  95% of sequence in fragments shorter than    1428 bp

# ApoI.MboII (RAATTY+GAAGA) double digest, 15581 thousand fragments (6382433+9199496 sites), 191 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     184 bp
  30% of sequence in fragments shorter than     213 bp
  35% of sequence in fragments shorter than     244 bp
  40% of sequence in fragments shorter than     277 bp
  45% of sequence in fragments shorter than     312 bp
  50% of sequence in fragments shorter than     347 bp
  55% of sequence in fragments shorter than     387 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     478 bp
  70% of sequence in fragments shorter than     531 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     672 bp
  85% of sequence in fragments shorter than     774 bp
  90% of sequence in fragments shorter than     914 bp
  95% of sequence in fragments shorter than    1165 bp

# BsmAI.Hpy188I (GTCTC+TCNGA) double digest, 15573 thousand fragments (6631585+8942153 sites), 191 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     180 bp
  30% of sequence in fragments shorter than     205 bp
  35% of sequence in fragments shorter than     234 bp
  40% of sequence in fragments shorter than     264 bp
  45% of sequence in fragments shorter than     296 bp
  50% of sequence in fragments shorter than     329 bp
  55% of sequence in fragments shorter than     361 bp
  60% of sequence in fragments shorter than     400 bp
  65% of sequence in fragments shorter than     443 bp
  70% of sequence in fragments shorter than     492 bp
  75% of sequence in fragments shorter than     546 bp
  80% of sequence in fragments shorter than     613 bp
  85% of sequence in fragments shorter than     701 bp
  90% of sequence in fragments shorter than     823 bp
  95% of sequence in fragments shorter than    1028 bp

# CviAII.HhaI (CATG+GCGC) double digest, 15571 thousand fragments (13815697+1756502 sites), 191 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     129 bp
  20% of sequence in fragments shorter than     156 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     276 bp
  45% of sequence in fragments shorter than     306 bp
  50% of sequence in fragments shorter than     338 bp
  55% of sequence in fragments shorter than     372 bp
  60% of sequence in fragments shorter than     412 bp
  65% of sequence in fragments shorter than     455 bp
  70% of sequence in fragments shorter than     506 bp
  75% of sequence in fragments shorter than     564 bp
  80% of sequence in fragments shorter than     632 bp
  85% of sequence in fragments shorter than     714 bp
  90% of sequence in fragments shorter than     836 bp
  95% of sequence in fragments shorter than    1031 bp

# BsmAI.HinfI (GTCTC+GANTC) double digest, 15547 thousand fragments (6631585+8916054 sites), 191 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     187 bp
  30% of sequence in fragments shorter than     215 bp
  35% of sequence in fragments shorter than     247 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     314 bp
  50% of sequence in fragments shorter than     345 bp
  55% of sequence in fragments shorter than     379 bp
  60% of sequence in fragments shorter than     422 bp
  65% of sequence in fragments shorter than     469 bp
  70% of sequence in fragments shorter than     521 bp
  75% of sequence in fragments shorter than     582 bp
  80% of sequence in fragments shorter than     656 bp
  85% of sequence in fragments shorter than     747 bp
  90% of sequence in fragments shorter than     878 bp
  95% of sequence in fragments shorter than    1098 bp

# BscGI.DdeI (CCCGT+CTNAG) double digest, 15542 thousand fragments (1231070+14312045 sites), 191 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     127 bp
  20% of sequence in fragments shorter than     153 bp
  25% of sequence in fragments shorter than     178 bp
  30% of sequence in fragments shorter than     207 bp
  35% of sequence in fragments shorter than     236 bp
  40% of sequence in fragments shorter than     267 bp
  45% of sequence in fragments shorter than     298 bp
  50% of sequence in fragments shorter than     331 bp
  55% of sequence in fragments shorter than     367 bp
  60% of sequence in fragments shorter than     406 bp
  65% of sequence in fragments shorter than     451 bp
  70% of sequence in fragments shorter than     503 bp
  75% of sequence in fragments shorter than     563 bp
  80% of sequence in fragments shorter than     634 bp
  85% of sequence in fragments shorter than     728 bp
  90% of sequence in fragments shorter than     857 bp
  95% of sequence in fragments shorter than    1083 bp

# BfiI.CviAII (ACTGGG+CATG) double digest, 15521 thousand fragments (1706594+13815697 sites), 191 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than      97 bp
  15% of sequence in fragments shorter than     128 bp
  20% of sequence in fragments shorter than     156 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     214 bp
  35% of sequence in fragments shorter than     243 bp
  40% of sequence in fragments shorter than     272 bp
  45% of sequence in fragments shorter than     302 bp
  50% of sequence in fragments shorter than     334 bp
  55% of sequence in fragments shorter than     369 bp
  60% of sequence in fragments shorter than     408 bp
  65% of sequence in fragments shorter than     452 bp
  70% of sequence in fragments shorter than     502 bp
  75% of sequence in fragments shorter than     560 bp
  80% of sequence in fragments shorter than     629 bp
  85% of sequence in fragments shorter than     718 bp
  90% of sequence in fragments shorter than     850 bp
  95% of sequence in fragments shorter than    1079 bp

# CviAII.PsiI (CATG+TTATAA) double digest, 15463 thousand fragments (13815697+1647912 sites), 192 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than      98 bp
  15% of sequence in fragments shorter than     129 bp
  20% of sequence in fragments shorter than     157 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     214 bp
  35% of sequence in fragments shorter than     243 bp
  40% of sequence in fragments shorter than     272 bp
  45% of sequence in fragments shorter than     301 bp
  50% of sequence in fragments shorter than     332 bp
  55% of sequence in fragments shorter than     367 bp
  60% of sequence in fragments shorter than     405 bp
  65% of sequence in fragments shorter than     449 bp
  70% of sequence in fragments shorter than     498 bp
  75% of sequence in fragments shorter than     556 bp
  80% of sequence in fragments shorter than     625 bp
  85% of sequence in fragments shorter than     716 bp
  90% of sequence in fragments shorter than     851 bp
  95% of sequence in fragments shorter than    1090 bp

# BscAI.ScrFI (GCATC+CCNGG) double digest, 15457 thousand fragments (3652926+11805099 sites), 192 bp average fragment length
   5% of sequence in fragments shorter than      56 bp
  10% of sequence in fragments shorter than      87 bp
  15% of sequence in fragments shorter than     119 bp
  20% of sequence in fragments shorter than     152 bp
  25% of sequence in fragments shorter than     188 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     267 bp
  40% of sequence in fragments shorter than     311 bp
  45% of sequence in fragments shorter than     358 bp
  50% of sequence in fragments shorter than     409 bp
  55% of sequence in fragments shorter than     466 bp
  60% of sequence in fragments shorter than     529 bp
  65% of sequence in fragments shorter than     595 bp
  70% of sequence in fragments shorter than     677 bp
  75% of sequence in fragments shorter than     773 bp
  80% of sequence in fragments shorter than     895 bp
  85% of sequence in fragments shorter than    1061 bp
  90% of sequence in fragments shorter than    1298 bp
  95% of sequence in fragments shorter than    1732 bp

# CviAII.EsaBC3I (CATG+TCGA) double digest, 15418 thousand fragments (13815697+1603346 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than      98 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     188 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     247 bp
  40% of sequence in fragments shorter than     276 bp
  45% of sequence in fragments shorter than     305 bp
  50% of sequence in fragments shorter than     337 bp
  55% of sequence in fragments shorter than     372 bp
  60% of sequence in fragments shorter than     410 bp
  65% of sequence in fragments shorter than     452 bp
  70% of sequence in fragments shorter than     501 bp
  75% of sequence in fragments shorter than     558 bp
  80% of sequence in fragments shorter than     624 bp
  85% of sequence in fragments shorter than     710 bp
  90% of sequence in fragments shorter than     831 bp
  95% of sequence in fragments shorter than    1041 bp

# AccI.DdeI (GTMKAC+CTNAG) double digest, 15412 thousand fragments (1101063+14312045 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     156 bp
  25% of sequence in fragments shorter than     178 bp
  30% of sequence in fragments shorter than     207 bp
  35% of sequence in fragments shorter than     234 bp
  40% of sequence in fragments shorter than     264 bp
  45% of sequence in fragments shorter than     294 bp
  50% of sequence in fragments shorter than     326 bp
  55% of sequence in fragments shorter than     359 bp
  60% of sequence in fragments shorter than     397 bp
  65% of sequence in fragments shorter than     439 bp
  70% of sequence in fragments shorter than     488 bp
  75% of sequence in fragments shorter than     544 bp
  80% of sequence in fragments shorter than     610 bp
  85% of sequence in fragments shorter than     698 bp
  90% of sequence in fragments shorter than     817 bp
  95% of sequence in fragments shorter than    1033 bp

# BthCI.MboII (GCNGC+GAAGA) double digest, 15408 thousand fragments (6209929+9199496 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     193 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     257 bp
  40% of sequence in fragments shorter than     291 bp
  45% of sequence in fragments shorter than     325 bp
  50% of sequence in fragments shorter than     361 bp
  55% of sequence in fragments shorter than     404 bp
  60% of sequence in fragments shorter than     449 bp
  65% of sequence in fragments shorter than     500 bp
  70% of sequence in fragments shorter than     557 bp
  75% of sequence in fragments shorter than     623 bp
  80% of sequence in fragments shorter than     701 bp
  85% of sequence in fragments shorter than     802 bp
  90% of sequence in fragments shorter than     942 bp
  95% of sequence in fragments shorter than    1180 bp

# AluI.SspI (AGCT+AATATT) double digest, 15404 thousand fragments (13027778+2377267 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     211 bp
  35% of sequence in fragments shorter than     237 bp
  40% of sequence in fragments shorter than     264 bp
  45% of sequence in fragments shorter than     291 bp
  50% of sequence in fragments shorter than     321 bp
  55% of sequence in fragments shorter than     351 bp
  60% of sequence in fragments shorter than     386 bp
  65% of sequence in fragments shorter than     426 bp
  70% of sequence in fragments shorter than     469 bp
  75% of sequence in fragments shorter than     519 bp
  80% of sequence in fragments shorter than     581 bp
  85% of sequence in fragments shorter than     661 bp
  90% of sequence in fragments shorter than     771 bp
  95% of sequence in fragments shorter than     976 bp

# DdeI.PpuMI (CTNAG+RGGWCCY) double digest, 15396 thousand fragments (14312045+1085138 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     157 bp
  25% of sequence in fragments shorter than     179 bp
  30% of sequence in fragments shorter than     208 bp
  35% of sequence in fragments shorter than     238 bp
  40% of sequence in fragments shorter than     268 bp
  45% of sequence in fragments shorter than     299 bp
  50% of sequence in fragments shorter than     332 bp
  55% of sequence in fragments shorter than     366 bp
  60% of sequence in fragments shorter than     407 bp
  65% of sequence in fragments shorter than     451 bp
  70% of sequence in fragments shorter than     503 bp
  75% of sequence in fragments shorter than     562 bp
  80% of sequence in fragments shorter than     633 bp
  85% of sequence in fragments shorter than     727 bp
  90% of sequence in fragments shorter than     856 bp
  95% of sequence in fragments shorter than    1082 bp

# BsmAI.TspRI (GTCTC+CASTG) double digest, 15396 thousand fragments (6631585+8765240 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      62 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     221 bp
  35% of sequence in fragments shorter than     254 bp
  40% of sequence in fragments shorter than     289 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     365 bp
  55% of sequence in fragments shorter than     407 bp
  60% of sequence in fragments shorter than     454 bp
  65% of sequence in fragments shorter than     507 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     634 bp
  80% of sequence in fragments shorter than     717 bp
  85% of sequence in fragments shorter than     829 bp
  90% of sequence in fragments shorter than     980 bp
  95% of sequence in fragments shorter than    1251 bp

# DdeI.PvuII (CTNAG+CAGCTG) double digest, 15395 thousand fragments (14312045+1084260 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     180 bp
  30% of sequence in fragments shorter than     210 bp
  35% of sequence in fragments shorter than     239 bp
  40% of sequence in fragments shorter than     270 bp
  45% of sequence in fragments shorter than     301 bp
  50% of sequence in fragments shorter than     334 bp
  55% of sequence in fragments shorter than     369 bp
  60% of sequence in fragments shorter than     408 bp
  65% of sequence in fragments shorter than     453 bp
  70% of sequence in fragments shorter than     504 bp
  75% of sequence in fragments shorter than     563 bp
  80% of sequence in fragments shorter than     634 bp
  85% of sequence in fragments shorter than     727 bp
  90% of sequence in fragments shorter than     854 bp
  95% of sequence in fragments shorter than    1079 bp

# BccI.MboII (CCATC+GAAGA) double digest, 15369 thousand fragments (6170927+9199496 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     248 bp
  40% of sequence in fragments shorter than     278 bp
  45% of sequence in fragments shorter than     310 bp
  50% of sequence in fragments shorter than     343 bp
  55% of sequence in fragments shorter than     378 bp
  60% of sequence in fragments shorter than     417 bp
  65% of sequence in fragments shorter than     460 bp
  70% of sequence in fragments shorter than     509 bp
  75% of sequence in fragments shorter than     565 bp
  80% of sequence in fragments shorter than     633 bp
  85% of sequence in fragments shorter than     719 bp
  90% of sequence in fragments shorter than     839 bp
  95% of sequence in fragments shorter than    1036 bp

# CviAII.HgiCI (CATG+GGYRCC) double digest, 15366 thousand fragments (13815697+1550877 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than      98 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     188 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     276 bp
  45% of sequence in fragments shorter than     304 bp
  50% of sequence in fragments shorter than     337 bp
  55% of sequence in fragments shorter than     373 bp
  60% of sequence in fragments shorter than     412 bp
  65% of sequence in fragments shorter than     456 bp
  70% of sequence in fragments shorter than     506 bp
  75% of sequence in fragments shorter than     565 bp
  80% of sequence in fragments shorter than     634 bp
  85% of sequence in fragments shorter than     724 bp
  90% of sequence in fragments shorter than     851 bp
  95% of sequence in fragments shorter than    1092 bp

# BsmI.CviAII (GAATGC+CATG) double digest, 15364 thousand fragments (1549350+13815697 sites), 193 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     184 bp
  30% of sequence in fragments shorter than     214 bp
  35% of sequence in fragments shorter than     243 bp
  40% of sequence in fragments shorter than     273 bp
  45% of sequence in fragments shorter than     303 bp
  50% of sequence in fragments shorter than     334 bp
  55% of sequence in fragments shorter than     368 bp
  60% of sequence in fragments shorter than     407 bp
  65% of sequence in fragments shorter than     450 bp
  70% of sequence in fragments shorter than     499 bp
  75% of sequence in fragments shorter than     556 bp
  80% of sequence in fragments shorter than     623 bp
  85% of sequence in fragments shorter than     709 bp
  90% of sequence in fragments shorter than     831 bp
  95% of sequence in fragments shorter than    1030 bp

# AluI.HpaII (AGCT+CCGG) double digest, 15345 thousand fragments (13027778+2317722 sites), 194 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     248 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     312 bp
  50% of sequence in fragments shorter than     345 bp
  55% of sequence in fragments shorter than     382 bp
  60% of sequence in fragments shorter than     425 bp
  65% of sequence in fragments shorter than     470 bp
  70% of sequence in fragments shorter than     523 bp
  75% of sequence in fragments shorter than     585 bp
  80% of sequence in fragments shorter than     661 bp
  85% of sequence in fragments shorter than     754 bp
  90% of sequence in fragments shorter than     890 bp
  95% of sequence in fragments shorter than    1135 bp

# ScrFI.SfeI (CCNGG+CTRYAG) double digest, 15335 thousand fragments (11805099+3531009 sites), 194 bp average fragment length
   5% of sequence in fragments shorter than      52 bp
  10% of sequence in fragments shorter than      86 bp
  15% of sequence in fragments shorter than     120 bp
  20% of sequence in fragments shorter than     156 bp
  25% of sequence in fragments shorter than     195 bp
  30% of sequence in fragments shorter than     236 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     325 bp
  45% of sequence in fragments shorter than     374 bp
  50% of sequence in fragments shorter than     428 bp
  55% of sequence in fragments shorter than     487 bp
  60% of sequence in fragments shorter than     553 bp
  65% of sequence in fragments shorter than     625 bp
  70% of sequence in fragments shorter than     705 bp
  75% of sequence in fragments shorter than     804 bp
  80% of sequence in fragments shorter than     931 bp
  85% of sequence in fragments shorter than    1094 bp
  90% of sequence in fragments shorter than    1336 bp
  95% of sequence in fragments shorter than    1754 bp

# ApoI.Hpy188I (RAATTY+TCNGA) double digest, 15324 thousand fragments (6382433+8942153 sites), 194 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     214 bp
  35% of sequence in fragments shorter than     245 bp
  40% of sequence in fragments shorter than     277 bp
  45% of sequence in fragments shorter than     311 bp
  50% of sequence in fragments shorter than     345 bp
  55% of sequence in fragments shorter than     383 bp
  60% of sequence in fragments shorter than     426 bp
  65% of sequence in fragments shorter than     473 bp
  70% of sequence in fragments shorter than     527 bp
  75% of sequence in fragments shorter than     590 bp
  80% of sequence in fragments shorter than     667 bp
  85% of sequence in fragments shorter than     764 bp
  90% of sequence in fragments shorter than     897 bp
  95% of sequence in fragments shorter than    1117 bp

# AvaI.DdeI (CYCGRG+CTNAG) double digest, 15317 thousand fragments (1005714+14312045 sites), 194 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     212 bp
  35% of sequence in fragments shorter than     243 bp
  40% of sequence in fragments shorter than     274 bp
  45% of sequence in fragments shorter than     306 bp
  50% of sequence in fragments shorter than     340 bp
  55% of sequence in fragments shorter than     375 bp
  60% of sequence in fragments shorter than     415 bp
  65% of sequence in fragments shorter than     461 bp
  70% of sequence in fragments shorter than     514 bp
  75% of sequence in fragments shorter than     573 bp
  80% of sequence in fragments shorter than     647 bp
  85% of sequence in fragments shorter than     740 bp
  90% of sequence in fragments shorter than     874 bp
  95% of sequence in fragments shorter than    1105 bp

# AflIII.CviAII (ACRYGT+CATG) double digest, 15300 thousand fragments (1485394+13815697 sites), 194 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     204 bp
  30% of sequence in fragments shorter than     235 bp
  35% of sequence in fragments shorter than     266 bp
  40% of sequence in fragments shorter than     297 bp
  45% of sequence in fragments shorter than     329 bp
  50% of sequence in fragments shorter than     365 bp
  55% of sequence in fragments shorter than     405 bp
  60% of sequence in fragments shorter than     447 bp
  65% of sequence in fragments shorter than     496 bp
  70% of sequence in fragments shorter than     550 bp
  75% of sequence in fragments shorter than     612 bp
  80% of sequence in fragments shorter than     688 bp
  85% of sequence in fragments shorter than     788 bp
  90% of sequence in fragments shorter than     933 bp
  95% of sequence in fragments shorter than    1181 bp

# ApoI.HinfI (RAATTY+GANTC) double digest, 15297 thousand fragments (6382433+8916054 sites), 194 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than      97 bp
  15% of sequence in fragments shorter than     129 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     211 bp
  35% of sequence in fragments shorter than     240 bp
  40% of sequence in fragments shorter than     268 bp
  45% of sequence in fragments shorter than     297 bp
  50% of sequence in fragments shorter than     326 bp
  55% of sequence in fragments shorter than     356 bp
  60% of sequence in fragments shorter than     392 bp
  65% of sequence in fragments shorter than     433 bp
  70% of sequence in fragments shorter than     479 bp
  75% of sequence in fragments shorter than     530 bp
  80% of sequence in fragments shorter than     595 bp
  85% of sequence in fragments shorter than     675 bp
  90% of sequence in fragments shorter than     788 bp
  95% of sequence in fragments shorter than     983 bp

# BspHI.DdeI (TCATGA+CTNAG) double digest, 15289 thousand fragments (978290+14312045 sites), 194 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     157 bp
  25% of sequence in fragments shorter than     178 bp
  30% of sequence in fragments shorter than     207 bp
  35% of sequence in fragments shorter than     236 bp
  40% of sequence in fragments shorter than     266 bp
  45% of sequence in fragments shorter than     296 bp
  50% of sequence in fragments shorter than     328 bp
  55% of sequence in fragments shorter than     361 bp
  60% of sequence in fragments shorter than     400 bp
  65% of sequence in fragments shorter than     443 bp
  70% of sequence in fragments shorter than     493 bp
  75% of sequence in fragments shorter than     550 bp
  80% of sequence in fragments shorter than     617 bp
  85% of sequence in fragments shorter than     707 bp
  90% of sequence in fragments shorter than     825 bp
  95% of sequence in fragments shorter than    1045 bp

# CviAII.FauI (CATG+CCCGC) double digest, 15254 thousand fragments (13815697+1439772 sites), 195 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     191 bp
  30% of sequence in fragments shorter than     221 bp
  35% of sequence in fragments shorter than     250 bp
  40% of sequence in fragments shorter than     281 bp
  45% of sequence in fragments shorter than     311 bp
  50% of sequence in fragments shorter than     344 bp
  55% of sequence in fragments shorter than     381 bp
  60% of sequence in fragments shorter than     421 bp
  65% of sequence in fragments shorter than     466 bp
  70% of sequence in fragments shorter than     517 bp
  75% of sequence in fragments shorter than     577 bp
  80% of sequence in fragments shorter than     648 bp
  85% of sequence in fragments shorter than     740 bp
  90% of sequence in fragments shorter than     870 bp
  95% of sequence in fragments shorter than    1091 bp

# BspMI.CviAII (ACCTGC+CATG) double digest, 15253 thousand fragments (1438129+13815697 sites), 195 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     220 bp
  35% of sequence in fragments shorter than     249 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     309 bp
  50% of sequence in fragments shorter than     342 bp
  55% of sequence in fragments shorter than     376 bp
  60% of sequence in fragments shorter than     416 bp
  65% of sequence in fragments shorter than     460 bp
  70% of sequence in fragments shorter than     511 bp
  75% of sequence in fragments shorter than     569 bp
  80% of sequence in fragments shorter than     638 bp
  85% of sequence in fragments shorter than     726 bp
  90% of sequence in fragments shorter than     854 bp
  95% of sequence in fragments shorter than    1061 bp

# AvaIII.DdeI (ATGCAT+CTNAG) double digest, 15244 thousand fragments (933357+14312045 sites), 195 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     180 bp
  30% of sequence in fragments shorter than     208 bp
  35% of sequence in fragments shorter than     237 bp
  40% of sequence in fragments shorter than     267 bp
  45% of sequence in fragments shorter than     297 bp
  50% of sequence in fragments shorter than     329 bp
  55% of sequence in fragments shorter than     362 bp
  60% of sequence in fragments shorter than     401 bp
  65% of sequence in fragments shorter than     443 bp
  70% of sequence in fragments shorter than     492 bp
  75% of sequence in fragments shorter than     548 bp
  80% of sequence in fragments shorter than     614 bp
  85% of sequence in fragments shorter than     702 bp
  90% of sequence in fragments shorter than     822 bp
  95% of sequence in fragments shorter than    1040 bp

# DdeI.StuI (CTNAG+AGGCCT) double digest, 15237 thousand fragments (14312045+925729 sites), 195 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     213 bp
  35% of sequence in fragments shorter than     243 bp
  40% of sequence in fragments shorter than     274 bp
  45% of sequence in fragments shorter than     305 bp
  50% of sequence in fragments shorter than     338 bp
  55% of sequence in fragments shorter than     374 bp
  60% of sequence in fragments shorter than     414 bp
  65% of sequence in fragments shorter than     459 bp
  70% of sequence in fragments shorter than     512 bp
  75% of sequence in fragments shorter than     570 bp
  80% of sequence in fragments shorter than     643 bp
  85% of sequence in fragments shorter than     735 bp
  90% of sequence in fragments shorter than     863 bp
  95% of sequence in fragments shorter than    1087 bp

# DdeI.NdeI (CTNAG+CATATG) double digest, 15214 thousand fragments (14312045+903360 sites), 195 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     180 bp
  30% of sequence in fragments shorter than     209 bp
  35% of sequence in fragments shorter than     237 bp
  40% of sequence in fragments shorter than     266 bp
  45% of sequence in fragments shorter than     296 bp
  50% of sequence in fragments shorter than     328 bp
  55% of sequence in fragments shorter than     361 bp
  60% of sequence in fragments shorter than     399 bp
  65% of sequence in fragments shorter than     441 bp
  70% of sequence in fragments shorter than     489 bp
  75% of sequence in fragments shorter than     545 bp
  80% of sequence in fragments shorter than     610 bp
  85% of sequence in fragments shorter than     693 bp
  90% of sequence in fragments shorter than     812 bp
  95% of sequence in fragments shorter than    1017 bp

# HphI.MboII (GGTGA+GAAGA) double digest, 15206 thousand fragments (6007336+9199496 sites), 195 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     187 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     276 bp
  45% of sequence in fragments shorter than     306 bp
  50% of sequence in fragments shorter than     337 bp
  55% of sequence in fragments shorter than     371 bp
  60% of sequence in fragments shorter than     409 bp
  65% of sequence in fragments shorter than     450 bp
  70% of sequence in fragments shorter than     498 bp
  75% of sequence in fragments shorter than     552 bp
  80% of sequence in fragments shorter than     619 bp
  85% of sequence in fragments shorter than     700 bp
  90% of sequence in fragments shorter than     815 bp
  95% of sequence in fragments shorter than    1004 bp

# BsmAI.HaeIII (GTCTC+GGCC) double digest, 15193 thousand fragments (6631585+8562230 sites), 196 bp average fragment length
   5% of sequence in fragments shorter than      58 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     227 bp
  35% of sequence in fragments shorter than     266 bp
  40% of sequence in fragments shorter than     308 bp
  45% of sequence in fragments shorter than     347 bp
  50% of sequence in fragments shorter than     396 bp
  55% of sequence in fragments shorter than     451 bp
  60% of sequence in fragments shorter than     509 bp
  65% of sequence in fragments shorter than     575 bp
  70% of sequence in fragments shorter than     651 bp
  75% of sequence in fragments shorter than     740 bp
  80% of sequence in fragments shorter than     852 bp
  85% of sequence in fragments shorter than     988 bp
  90% of sequence in fragments shorter than    1182 bp
  95% of sequence in fragments shorter than    1525 bp

# DdeI.HindIII (CTNAG+AAGCTT) double digest, 15171 thousand fragments (14312045+860365 sites), 196 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     182 bp
  30% of sequence in fragments shorter than     211 bp
  35% of sequence in fragments shorter than     241 bp
  40% of sequence in fragments shorter than     271 bp
  45% of sequence in fragments shorter than     302 bp
  50% of sequence in fragments shorter than     335 bp
  55% of sequence in fragments shorter than     369 bp
  60% of sequence in fragments shorter than     407 bp
  65% of sequence in fragments shorter than     451 bp
  70% of sequence in fragments shorter than     500 bp
  75% of sequence in fragments shorter than     557 bp
  80% of sequence in fragments shorter than     625 bp
  85% of sequence in fragments shorter than     713 bp
  90% of sequence in fragments shorter than     835 bp
  95% of sequence in fragments shorter than    1047 bp

# DdeI.XbaI (CTNAG+TCTAGA) double digest, 15162 thousand fragments (14312045+850998 sites), 196 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     158 bp
  25% of sequence in fragments shorter than     182 bp
  30% of sequence in fragments shorter than     211 bp
  35% of sequence in fragments shorter than     240 bp
  40% of sequence in fragments shorter than     270 bp
  45% of sequence in fragments shorter than     300 bp
  50% of sequence in fragments shorter than     333 bp
  55% of sequence in fragments shorter than     367 bp
  60% of sequence in fragments shorter than     405 bp
  65% of sequence in fragments shorter than     449 bp
  70% of sequence in fragments shorter than     499 bp
  75% of sequence in fragments shorter than     556 bp
  80% of sequence in fragments shorter than     623 bp
  85% of sequence in fragments shorter than     712 bp
  90% of sequence in fragments shorter than     831 bp
  95% of sequence in fragments shorter than    1035 bp

# DdeI.EcoRI (CTNAG+GAATTC) double digest, 15158 thousand fragments (14312045+847401 sites), 196 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     182 bp
  30% of sequence in fragments shorter than     211 bp
  35% of sequence in fragments shorter than     241 bp
  40% of sequence in fragments shorter than     271 bp
  45% of sequence in fragments shorter than     302 bp
  50% of sequence in fragments shorter than     334 bp
  55% of sequence in fragments shorter than     369 bp
  60% of sequence in fragments shorter than     407 bp
  65% of sequence in fragments shorter than     451 bp
  70% of sequence in fragments shorter than     501 bp
  75% of sequence in fragments shorter than     559 bp
  80% of sequence in fragments shorter than     627 bp
  85% of sequence in fragments shorter than     715 bp
  90% of sequence in fragments shorter than     835 bp
  95% of sequence in fragments shorter than    1053 bp

# BthCI.Hpy188I (GCNGC+TCNGA) double digest, 15151 thousand fragments (6209929+8942153 sites), 196 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     193 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     258 bp
  40% of sequence in fragments shorter than     292 bp
  45% of sequence in fragments shorter than     329 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     407 bp
  60% of sequence in fragments shorter than     453 bp
  65% of sequence in fragments shorter than     505 bp
  70% of sequence in fragments shorter than     564 bp
  75% of sequence in fragments shorter than     632 bp
  80% of sequence in fragments shorter than     715 bp
  85% of sequence in fragments shorter than     821 bp
  90% of sequence in fragments shorter than     969 bp
  95% of sequence in fragments shorter than    1204 bp

# ApoI.TspRI (RAATTY+CASTG) double digest, 15147 thousand fragments (6382433+8765240 sites), 196 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     240 bp
  40% of sequence in fragments shorter than     267 bp
  45% of sequence in fragments shorter than     296 bp
  50% of sequence in fragments shorter than     327 bp
  55% of sequence in fragments shorter than     359 bp
  60% of sequence in fragments shorter than     396 bp
  65% of sequence in fragments shorter than     436 bp
  70% of sequence in fragments shorter than     481 bp
  75% of sequence in fragments shorter than     534 bp
  80% of sequence in fragments shorter than     595 bp
  85% of sequence in fragments shorter than     675 bp
  90% of sequence in fragments shorter than     783 bp
  95% of sequence in fragments shorter than     980 bp

# BbvI.BstNI (GCAGC+CCWGG) double digest, 15145 thousand fragments (5290051+9855646 sites), 196 bp average fragment length
   5% of sequence in fragments shorter than      58 bp
  10% of sequence in fragments shorter than      92 bp
  15% of sequence in fragments shorter than     125 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     194 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     275 bp
  40% of sequence in fragments shorter than     320 bp
  45% of sequence in fragments shorter than     367 bp
  50% of sequence in fragments shorter than     422 bp
  55% of sequence in fragments shorter than     481 bp
  60% of sequence in fragments shorter than     549 bp
  65% of sequence in fragments shorter than     624 bp
  70% of sequence in fragments shorter than     707 bp
  75% of sequence in fragments shorter than     815 bp
  80% of sequence in fragments shorter than     942 bp
  85% of sequence in fragments shorter than    1116 bp
  90% of sequence in fragments shorter than    1344 bp
  95% of sequence in fragments shorter than    1770 bp

# CviAII.PstI (CATG+CTGCAG) double digest, 15136 thousand fragments (13815697+1321469 sites), 196 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     131 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     220 bp
  35% of sequence in fragments shorter than     249 bp
  40% of sequence in fragments shorter than     278 bp
  45% of sequence in fragments shorter than     309 bp
  50% of sequence in fragments shorter than     341 bp
  55% of sequence in fragments shorter than     378 bp
  60% of sequence in fragments shorter than     418 bp
  65% of sequence in fragments shorter than     462 bp
  70% of sequence in fragments shorter than     512 bp
  75% of sequence in fragments shorter than     572 bp
  80% of sequence in fragments shorter than     642 bp
  85% of sequence in fragments shorter than     733 bp
  90% of sequence in fragments shorter than     866 bp
  95% of sequence in fragments shorter than    1091 bp

# BthCI.HinfI (GCNGC+GANTC) double digest, 15125 thousand fragments (6209929+8916054 sites), 197 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     197 bp
  30% of sequence in fragments shorter than     228 bp
  35% of sequence in fragments shorter than     259 bp
  40% of sequence in fragments shorter than     290 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     357 bp
  55% of sequence in fragments shorter than     395 bp
  60% of sequence in fragments shorter than     439 bp
  65% of sequence in fragments shorter than     486 bp
  70% of sequence in fragments shorter than     541 bp
  75% of sequence in fragments shorter than     606 bp
  80% of sequence in fragments shorter than     681 bp
  85% of sequence in fragments shorter than     775 bp
  90% of sequence in fragments shorter than     910 bp
  95% of sequence in fragments shorter than    1143 bp

# BccI.Hpy188I (CCATC+TCNGA) double digest, 15112 thousand fragments (6170927+8942153 sites), 197 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     245 bp
  40% of sequence in fragments shorter than     276 bp
  45% of sequence in fragments shorter than     307 bp
  50% of sequence in fragments shorter than     341 bp
  55% of sequence in fragments shorter than     374 bp
  60% of sequence in fragments shorter than     412 bp
  65% of sequence in fragments shorter than     454 bp
  70% of sequence in fragments shorter than     504 bp
  75% of sequence in fragments shorter than     560 bp
  80% of sequence in fragments shorter than     626 bp
  85% of sequence in fragments shorter than     712 bp
  90% of sequence in fragments shorter than     830 bp
  95% of sequence in fragments shorter than    1030 bp

# BccI.HinfI (CCATC+GANTC) double digest, 15086 thousand fragments (6170927+8916054 sites), 197 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     276 bp
  45% of sequence in fragments shorter than     307 bp
  50% of sequence in fragments shorter than     340 bp
  55% of sequence in fragments shorter than     371 bp
  60% of sequence in fragments shorter than     411 bp
  65% of sequence in fragments shorter than     453 bp
  70% of sequence in fragments shorter than     503 bp
  75% of sequence in fragments shorter than     561 bp
  80% of sequence in fragments shorter than     631 bp
  85% of sequence in fragments shorter than     717 bp
  90% of sequence in fragments shorter than     840 bp
  95% of sequence in fragments shorter than    1048 bp

# BglII.DdeI (AGATCT+CTNAG) double digest, 15086 thousand fragments (774732+14312045 sites), 197 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     212 bp
  35% of sequence in fragments shorter than     242 bp
  40% of sequence in fragments shorter than     272 bp
  45% of sequence in fragments shorter than     303 bp
  50% of sequence in fragments shorter than     336 bp
  55% of sequence in fragments shorter than     370 bp
  60% of sequence in fragments shorter than     409 bp
  65% of sequence in fragments shorter than     453 bp
  70% of sequence in fragments shorter than     504 bp
  75% of sequence in fragments shorter than     562 bp
  80% of sequence in fragments shorter than     633 bp
  85% of sequence in fragments shorter than     725 bp
  90% of sequence in fragments shorter than     851 bp
  95% of sequence in fragments shorter than    1072 bp

# DdeI.NcoI (CTNAG+CCATGG) double digest, 15071 thousand fragments (14312045+759594 sites), 197 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     182 bp
  30% of sequence in fragments shorter than     211 bp
  35% of sequence in fragments shorter than     240 bp
  40% of sequence in fragments shorter than     271 bp
  45% of sequence in fragments shorter than     303 bp
  50% of sequence in fragments shorter than     337 bp
  55% of sequence in fragments shorter than     371 bp
  60% of sequence in fragments shorter than     411 bp
  65% of sequence in fragments shorter than     455 bp
  70% of sequence in fragments shorter than     507 bp
  75% of sequence in fragments shorter than     565 bp
  80% of sequence in fragments shorter than     637 bp
  85% of sequence in fragments shorter than     729 bp
  90% of sequence in fragments shorter than     858 bp
  95% of sequence in fragments shorter than    1082 bp

# BstNI.FokI (CCWGG+GGATG) double digest, 15056 thousand fragments (9855646+5201116 sites), 197 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     227 bp
  35% of sequence in fragments shorter than     265 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     347 bp
  50% of sequence in fragments shorter than     393 bp
  55% of sequence in fragments shorter than     445 bp
  60% of sequence in fragments shorter than     503 bp
  65% of sequence in fragments shorter than     570 bp
  70% of sequence in fragments shorter than     644 bp
  75% of sequence in fragments shorter than     735 bp
  80% of sequence in fragments shorter than     847 bp
  85% of sequence in fragments shorter than     990 bp
  90% of sequence in fragments shorter than    1199 bp
  95% of sequence in fragments shorter than    1581 bp

# BclI.DdeI (TGATCA+CTNAG) double digest, 15050 thousand fragments (738785+14312045 sites), 198 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     212 bp
  35% of sequence in fragments shorter than     241 bp
  40% of sequence in fragments shorter than     272 bp
  45% of sequence in fragments shorter than     303 bp
  50% of sequence in fragments shorter than     336 bp
  55% of sequence in fragments shorter than     370 bp
  60% of sequence in fragments shorter than     408 bp
  65% of sequence in fragments shorter than     452 bp
  70% of sequence in fragments shorter than     500 bp
  75% of sequence in fragments shorter than     557 bp
  80% of sequence in fragments shorter than     625 bp
  85% of sequence in fragments shorter than     713 bp
  90% of sequence in fragments shorter than     835 bp
  95% of sequence in fragments shorter than    1048 bp

# BscGI.CviAII (CCCGT+CATG) double digest, 15046 thousand fragments (1231070+13815697 sites), 198 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     194 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     252 bp
  40% of sequence in fragments shorter than     283 bp
  45% of sequence in fragments shorter than     313 bp
  50% of sequence in fragments shorter than     344 bp
  55% of sequence in fragments shorter than     377 bp
  60% of sequence in fragments shorter than     416 bp
  65% of sequence in fragments shorter than     460 bp
  70% of sequence in fragments shorter than     509 bp
  75% of sequence in fragments shorter than     565 bp
  80% of sequence in fragments shorter than     632 bp
  85% of sequence in fragments shorter than     717 bp
  90% of sequence in fragments shorter than     837 bp
  95% of sequence in fragments shorter than    1034 bp

# DdeI.FnuDII (CTNAG+CGCG) double digest, 15045 thousand fragments (14312045+733940 sites), 198 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     213 bp
  35% of sequence in fragments shorter than     244 bp
  40% of sequence in fragments shorter than     276 bp
  45% of sequence in fragments shorter than     309 bp
  50% of sequence in fragments shorter than     343 bp
  55% of sequence in fragments shorter than     379 bp
  60% of sequence in fragments shorter than     420 bp
  65% of sequence in fragments shorter than     467 bp
  70% of sequence in fragments shorter than     520 bp
  75% of sequence in fragments shorter than     580 bp
  80% of sequence in fragments shorter than     654 bp
  85% of sequence in fragments shorter than     749 bp
  90% of sequence in fragments shorter than     884 bp
  95% of sequence in fragments shorter than    1116 bp

# BsaAI.DdeI (YACGTR+CTNAG) double digest, 15025 thousand fragments (713801+14312045 sites), 198 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     212 bp
  35% of sequence in fragments shorter than     241 bp
  40% of sequence in fragments shorter than     272 bp
  45% of sequence in fragments shorter than     303 bp
  50% of sequence in fragments shorter than     336 bp
  55% of sequence in fragments shorter than     371 bp
  60% of sequence in fragments shorter than     410 bp
  65% of sequence in fragments shorter than     454 bp
  70% of sequence in fragments shorter than     505 bp
  75% of sequence in fragments shorter than     563 bp
  80% of sequence in fragments shorter than     634 bp
  85% of sequence in fragments shorter than     726 bp
  90% of sequence in fragments shorter than     852 bp
  95% of sequence in fragments shorter than    1073 bp

# BceAI.DdeI (ACGGC+CTNAG) double digest, 15016 thousand fragments (705178+14312045 sites), 198 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     160 bp
  25% of sequence in fragments shorter than     183 bp
  30% of sequence in fragments shorter than     213 bp
  35% of sequence in fragments shorter than     243 bp
  40% of sequence in fragments shorter than     274 bp
  45% of sequence in fragments shorter than     306 bp
  50% of sequence in fragments shorter than     339 bp
  55% of sequence in fragments shorter than     375 bp
  60% of sequence in fragments shorter than     414 bp
  65% of sequence in fragments shorter than     460 bp
  70% of sequence in fragments shorter than     512 bp
  75% of sequence in fragments shorter than     570 bp
  80% of sequence in fragments shorter than     644 bp
  85% of sequence in fragments shorter than     736 bp
  90% of sequence in fragments shorter than     869 bp
  95% of sequence in fragments shorter than    1097 bp

# BciVI.DdeI (GTATCC+CTNAG) double digest, 15007 thousand fragments (696093+14312045 sites), 198 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     184 bp
  30% of sequence in fragments shorter than     212 bp
  35% of sequence in fragments shorter than     242 bp
  40% of sequence in fragments shorter than     273 bp
  45% of sequence in fragments shorter than     303 bp
  50% of sequence in fragments shorter than     337 bp
  55% of sequence in fragments shorter than     371 bp
  60% of sequence in fragments shorter than     410 bp
  65% of sequence in fragments shorter than     455 bp
  70% of sequence in fragments shorter than     506 bp
  75% of sequence in fragments shorter than     563 bp
  80% of sequence in fragments shorter than     633 bp
  85% of sequence in fragments shorter than     724 bp
  90% of sequence in fragments shorter than     849 bp
  95% of sequence in fragments shorter than    1066 bp

# BthCI.TspRI (GCNGC+CASTG) double digest, 14974 thousand fragments (6209929+8765240 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     211 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     309 bp
  45% of sequence in fragments shorter than     346 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     432 bp
  60% of sequence in fragments shorter than     482 bp
  65% of sequence in fragments shorter than     538 bp
  70% of sequence in fragments shorter than     601 bp
  75% of sequence in fragments shorter than     676 bp
  80% of sequence in fragments shorter than     762 bp
  85% of sequence in fragments shorter than     870 bp
  90% of sequence in fragments shorter than    1030 bp
  95% of sequence in fragments shorter than    1297 bp

# BstNI.CviQI (CCWGG+GTAC) double digest, 14970 thousand fragments (9855646+5115078 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      97 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     157 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     252 bp
  40% of sequence in fragments shorter than     288 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     407 bp
  60% of sequence in fragments shorter than     456 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     571 bp
  75% of sequence in fragments shorter than     644 bp
  80% of sequence in fragments shorter than     732 bp
  85% of sequence in fragments shorter than     852 bp
  90% of sequence in fragments shorter than    1027 bp
  95% of sequence in fragments shorter than    1336 bp

# AflII.DdeI (CTTAAG+CTNAG) double digest, 14954 thousand fragments (643284+14312045 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     184 bp
  30% of sequence in fragments shorter than     213 bp
  35% of sequence in fragments shorter than     243 bp
  40% of sequence in fragments shorter than     274 bp
  45% of sequence in fragments shorter than     305 bp
  50% of sequence in fragments shorter than     338 bp
  55% of sequence in fragments shorter than     372 bp
  60% of sequence in fragments shorter than     412 bp
  65% of sequence in fragments shorter than     456 bp
  70% of sequence in fragments shorter than     507 bp
  75% of sequence in fragments shorter than     565 bp
  80% of sequence in fragments shorter than     636 bp
  85% of sequence in fragments shorter than     728 bp
  90% of sequence in fragments shorter than     854 bp
  95% of sequence in fragments shorter than    1077 bp

# HphI.Hpy188I (GGTGA+TCNGA) double digest, 14948 thousand fragments (6007336+8942153 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     184 bp
  30% of sequence in fragments shorter than     213 bp
  35% of sequence in fragments shorter than     241 bp
  40% of sequence in fragments shorter than     271 bp
  45% of sequence in fragments shorter than     302 bp
  50% of sequence in fragments shorter than     334 bp
  55% of sequence in fragments shorter than     365 bp
  60% of sequence in fragments shorter than     403 bp
  65% of sequence in fragments shorter than     445 bp
  70% of sequence in fragments shorter than     492 bp
  75% of sequence in fragments shorter than     545 bp
  80% of sequence in fragments shorter than     611 bp
  85% of sequence in fragments shorter than     695 bp
  90% of sequence in fragments shorter than     807 bp
  95% of sequence in fragments shorter than     999 bp

# ApoI.HaeIII (RAATTY+GGCC) double digest, 14944 thousand fragments (6382433+8562230 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     195 bp
  30% of sequence in fragments shorter than     225 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     350 bp
  55% of sequence in fragments shorter than     387 bp
  60% of sequence in fragments shorter than     426 bp
  65% of sequence in fragments shorter than     471 bp
  70% of sequence in fragments shorter than     520 bp
  75% of sequence in fragments shorter than     578 bp
  80% of sequence in fragments shorter than     644 bp
  85% of sequence in fragments shorter than     728 bp
  90% of sequence in fragments shorter than     850 bp
  95% of sequence in fragments shorter than    1049 bp

# DdeI.HaeII (CTNAG+RGCGCY) double digest, 14941 thousand fragments (14312045+630101 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     132 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     278 bp
  45% of sequence in fragments shorter than     310 bp
  50% of sequence in fragments shorter than     344 bp
  55% of sequence in fragments shorter than     381 bp
  60% of sequence in fragments shorter than     421 bp
  65% of sequence in fragments shorter than     468 bp
  70% of sequence in fragments shorter than     521 bp
  75% of sequence in fragments shorter than     580 bp
  80% of sequence in fragments shorter than     655 bp
  85% of sequence in fragments shorter than     749 bp
  90% of sequence in fragments shorter than     883 bp
  95% of sequence in fragments shorter than    1116 bp

# BsrI.MboII (ACTGG+GAAGA) double digest, 14940 thousand fragments (5741310+9199496 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     221 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     352 bp
  55% of sequence in fragments shorter than     390 bp
  60% of sequence in fragments shorter than     432 bp
  65% of sequence in fragments shorter than     479 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     592 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     758 bp
  90% of sequence in fragments shorter than     889 bp
  95% of sequence in fragments shorter than    1111 bp

# BccI.TspRI (CCATC+CASTG) double digest, 14935 thousand fragments (6170927+8765240 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     194 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     354 bp
  55% of sequence in fragments shorter than     394 bp
  60% of sequence in fragments shorter than     437 bp
  65% of sequence in fragments shorter than     485 bp
  70% of sequence in fragments shorter than     539 bp
  75% of sequence in fragments shorter than     605 bp
  80% of sequence in fragments shorter than     684 bp
  85% of sequence in fragments shorter than     787 bp
  90% of sequence in fragments shorter than     937 bp
  95% of sequence in fragments shorter than    1200 bp

# HinfI.HphI (GANTC+GGTGA) double digest, 14922 thousand fragments (8916054+6007336 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     188 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     247 bp
  40% of sequence in fragments shorter than     278 bp
  45% of sequence in fragments shorter than     310 bp
  50% of sequence in fragments shorter than     342 bp
  55% of sequence in fragments shorter than     375 bp
  60% of sequence in fragments shorter than     415 bp
  65% of sequence in fragments shorter than     459 bp
  70% of sequence in fragments shorter than     509 bp
  75% of sequence in fragments shorter than     567 bp
  80% of sequence in fragments shorter than     637 bp
  85% of sequence in fragments shorter than     724 bp
  90% of sequence in fragments shorter than     849 bp
  95% of sequence in fragments shorter than    1058 bp

# ScrFI.StyI (CCNGG+CCWWGG) double digest, 14918 thousand fragments (11805099+3114107 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      53 bp
  10% of sequence in fragments shorter than      87 bp
  15% of sequence in fragments shorter than     120 bp
  20% of sequence in fragments shorter than     157 bp
  25% of sequence in fragments shorter than     197 bp
  30% of sequence in fragments shorter than     240 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     333 bp
  45% of sequence in fragments shorter than     387 bp
  50% of sequence in fragments shorter than     447 bp
  55% of sequence in fragments shorter than     511 bp
  60% of sequence in fragments shorter than     586 bp
  65% of sequence in fragments shorter than     665 bp
  70% of sequence in fragments shorter than     755 bp
  75% of sequence in fragments shorter than     871 bp
  80% of sequence in fragments shorter than    1018 bp
  85% of sequence in fragments shorter than    1215 bp
  90% of sequence in fragments shorter than    1496 bp
  95% of sequence in fragments shorter than    2063 bp

# AccI.CviAII (GTMKAC+CATG) double digest, 14916 thousand fragments (1101063+13815697 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     195 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     314 bp
  50% of sequence in fragments shorter than     347 bp
  55% of sequence in fragments shorter than     384 bp
  60% of sequence in fragments shorter than     425 bp
  65% of sequence in fragments shorter than     470 bp
  70% of sequence in fragments shorter than     521 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     653 bp
  85% of sequence in fragments shorter than     746 bp
  90% of sequence in fragments shorter than     880 bp
  95% of sequence in fragments shorter than    1116 bp

# DdeI.SacI (CTNAG+GAGCTC) double digest, 14914 thousand fragments (14312045+603323 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     187 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     248 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     311 bp
  50% of sequence in fragments shorter than     345 bp
  55% of sequence in fragments shorter than     381 bp
  60% of sequence in fragments shorter than     421 bp
  65% of sequence in fragments shorter than     467 bp
  70% of sequence in fragments shorter than     520 bp
  75% of sequence in fragments shorter than     578 bp
  80% of sequence in fragments shorter than     653 bp
  85% of sequence in fragments shorter than     746 bp
  90% of sequence in fragments shorter than     879 bp
  95% of sequence in fragments shorter than    1110 bp

# NspI.ScrFI (RCATGY+CCNGG) double digest, 14908 thousand fragments (3104359+11805099 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      56 bp
  10% of sequence in fragments shorter than      88 bp
  15% of sequence in fragments shorter than     123 bp
  20% of sequence in fragments shorter than     159 bp
  25% of sequence in fragments shorter than     197 bp
  30% of sequence in fragments shorter than     235 bp
  35% of sequence in fragments shorter than     277 bp
  40% of sequence in fragments shorter than     322 bp
  45% of sequence in fragments shorter than     368 bp
  50% of sequence in fragments shorter than     418 bp
  55% of sequence in fragments shorter than     475 bp
  60% of sequence in fragments shorter than     539 bp
  65% of sequence in fragments shorter than     610 bp
  70% of sequence in fragments shorter than     691 bp
  75% of sequence in fragments shorter than     790 bp
  80% of sequence in fragments shorter than     914 bp
  85% of sequence in fragments shorter than    1074 bp
  90% of sequence in fragments shorter than    1314 bp
  95% of sequence in fragments shorter than    1747 bp

# AvrII.DdeI (CCTAGG+CTNAG) double digest, 14906 thousand fragments (594956+14312045 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     184 bp
  30% of sequence in fragments shorter than     214 bp
  35% of sequence in fragments shorter than     244 bp
  40% of sequence in fragments shorter than     275 bp
  45% of sequence in fragments shorter than     306 bp
  50% of sequence in fragments shorter than     340 bp
  55% of sequence in fragments shorter than     374 bp
  60% of sequence in fragments shorter than     415 bp
  65% of sequence in fragments shorter than     461 bp
  70% of sequence in fragments shorter than     513 bp
  75% of sequence in fragments shorter than     569 bp
  80% of sequence in fragments shorter than     641 bp
  85% of sequence in fragments shorter than     734 bp
  90% of sequence in fragments shorter than     862 bp
  95% of sequence in fragments shorter than    1093 bp

# CviAII.PpuMI (CATG+RGGWCCY) double digest, 14900 thousand fragments (13815697+1085138 sites), 199 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     194 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     314 bp
  50% of sequence in fragments shorter than     347 bp
  55% of sequence in fragments shorter than     384 bp
  60% of sequence in fragments shorter than     425 bp
  65% of sequence in fragments shorter than     470 bp
  70% of sequence in fragments shorter than     522 bp
  75% of sequence in fragments shorter than     582 bp
  80% of sequence in fragments shorter than     654 bp
  85% of sequence in fragments shorter than     747 bp
  90% of sequence in fragments shorter than     885 bp
  95% of sequence in fragments shorter than    1121 bp

# CviAII.PvuII (CATG+CAGCTG) double digest, 14899 thousand fragments (13815697+1084260 sites), 200 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     193 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     252 bp
  40% of sequence in fragments shorter than     283 bp
  45% of sequence in fragments shorter than     313 bp
  50% of sequence in fragments shorter than     346 bp
  55% of sequence in fragments shorter than     383 bp
  60% of sequence in fragments shorter than     423 bp
  65% of sequence in fragments shorter than     469 bp
  70% of sequence in fragments shorter than     521 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     653 bp
  85% of sequence in fragments shorter than     746 bp
  90% of sequence in fragments shorter than     882 bp
  95% of sequence in fragments shorter than    1118 bp

# DdeI.HgaI (CTNAG+GACGC) double digest, 14883 thousand fragments (14312045+571889 sites), 200 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     215 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     277 bp
  45% of sequence in fragments shorter than     309 bp
  50% of sequence in fragments shorter than     343 bp
  55% of sequence in fragments shorter than     378 bp
  60% of sequence in fragments shorter than     418 bp
  65% of sequence in fragments shorter than     464 bp
  70% of sequence in fragments shorter than     517 bp
  75% of sequence in fragments shorter than     575 bp
  80% of sequence in fragments shorter than     649 bp
  85% of sequence in fragments shorter than     741 bp
  90% of sequence in fragments shorter than     875 bp
  95% of sequence in fragments shorter than    1099 bp

# DdeI.EciI (CTNAG+GGCGGA) double digest, 14882 thousand fragments (14312045+571273 sites), 200 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     247 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     311 bp
  50% of sequence in fragments shorter than     345 bp
  55% of sequence in fragments shorter than     382 bp
  60% of sequence in fragments shorter than     423 bp
  65% of sequence in fragments shorter than     470 bp
  70% of sequence in fragments shorter than     523 bp
  75% of sequence in fragments shorter than     583 bp
  80% of sequence in fragments shorter than     658 bp
  85% of sequence in fragments shorter than     752 bp
  90% of sequence in fragments shorter than     887 bp
  95% of sequence in fragments shorter than    1120 bp

# DdeI.MfeI (CTNAG+CAATTG) double digest, 14875 thousand fragments (14312045+564303 sites), 200 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     162 bp
  25% of sequence in fragments shorter than     185 bp
  30% of sequence in fragments shorter than     214 bp
  35% of sequence in fragments shorter than     244 bp
  40% of sequence in fragments shorter than     275 bp
  45% of sequence in fragments shorter than     306 bp
  50% of sequence in fragments shorter than     338 bp
  55% of sequence in fragments shorter than     373 bp
  60% of sequence in fragments shorter than     413 bp
  65% of sequence in fragments shorter than     457 bp
  70% of sequence in fragments shorter than     508 bp
  75% of sequence in fragments shorter than     566 bp
  80% of sequence in fragments shorter than     636 bp
  85% of sequence in fragments shorter than     728 bp
  90% of sequence in fragments shorter than     852 bp
  95% of sequence in fragments shorter than    1071 bp

# DdeI.SphI (CTNAG+GCATGC) double digest, 14862 thousand fragments (14312045+550952 sites), 200 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     247 bp
  40% of sequence in fragments shorter than     278 bp
  45% of sequence in fragments shorter than     310 bp
  50% of sequence in fragments shorter than     343 bp
  55% of sequence in fragments shorter than     379 bp
  60% of sequence in fragments shorter than     419 bp
  65% of sequence in fragments shorter than     464 bp
  70% of sequence in fragments shorter than     517 bp
  75% of sequence in fragments shorter than     575 bp
  80% of sequence in fragments shorter than     648 bp
  85% of sequence in fragments shorter than     741 bp
  90% of sequence in fragments shorter than     873 bp
  95% of sequence in fragments shorter than    1101 bp

# DdeI.ScaI (CTNAG+AGTACT) double digest, 14855 thousand fragments (14312045+543793 sites), 200 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     215 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     277 bp
  45% of sequence in fragments shorter than     308 bp
  50% of sequence in fragments shorter than     342 bp
  55% of sequence in fragments shorter than     377 bp
  60% of sequence in fragments shorter than     416 bp
  65% of sequence in fragments shorter than     462 bp
  70% of sequence in fragments shorter than     513 bp
  75% of sequence in fragments shorter than     571 bp
  80% of sequence in fragments shorter than     643 bp
  85% of sequence in fragments shorter than     733 bp
  90% of sequence in fragments shorter than     863 bp
  95% of sequence in fragments shorter than    1086 bp

# BstNI.TfiI (CCWGG+GAWTC) double digest, 14839 thousand fragments (9855646+4984362 sites), 200 bp average fragment length
   5% of sequence in fragments shorter than      62 bp
  10% of sequence in fragments shorter than      98 bp
  15% of sequence in fragments shorter than     127 bp
  20% of sequence in fragments shorter than     161 bp
  25% of sequence in fragments shorter than     191 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     299 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     372 bp
  55% of sequence in fragments shorter than     417 bp
  60% of sequence in fragments shorter than     469 bp
  65% of sequence in fragments shorter than     525 bp
  70% of sequence in fragments shorter than     591 bp
  75% of sequence in fragments shorter than     669 bp
  80% of sequence in fragments shorter than     757 bp
  85% of sequence in fragments shorter than     876 bp
  90% of sequence in fragments shorter than    1043 bp
  95% of sequence in fragments shorter than    1331 bp

# AvaI.CviAII (CYCGRG+CATG) double digest, 14820 thousand fragments (1005714+13815697 sites), 201 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     195 bp
  30% of sequence in fragments shorter than     225 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     316 bp
  50% of sequence in fragments shorter than     350 bp
  55% of sequence in fragments shorter than     387 bp
  60% of sequence in fragments shorter than     428 bp
  65% of sequence in fragments shorter than     474 bp
  70% of sequence in fragments shorter than     526 bp
  75% of sequence in fragments shorter than     586 bp
  80% of sequence in fragments shorter than     659 bp
  85% of sequence in fragments shorter than     753 bp
  90% of sequence in fragments shorter than     890 bp
  95% of sequence in fragments shorter than    1129 bp

# ApaLI.DdeI (GTGCAC+CTNAG) double digest, 14807 thousand fragments (496255+14312045 sites), 201 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     246 bp
  40% of sequence in fragments shorter than     277 bp
  45% of sequence in fragments shorter than     309 bp
  50% of sequence in fragments shorter than     343 bp
  55% of sequence in fragments shorter than     378 bp
  60% of sequence in fragments shorter than     418 bp
  65% of sequence in fragments shorter than     464 bp
  70% of sequence in fragments shorter than     517 bp
  75% of sequence in fragments shorter than     575 bp
  80% of sequence in fragments shorter than     649 bp
  85% of sequence in fragments shorter than     741 bp
  90% of sequence in fragments shorter than     874 bp
  95% of sequence in fragments shorter than    1102 bp

# BspHI.CviAII (TCATGA+CATG) double digest, 14793 thousand fragments (978290+13815697 sites), 201 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     416 bp
  60% of sequence in fragments shorter than     460 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     629 bp
  80% of sequence in fragments shorter than     707 bp
  85% of sequence in fragments shorter than     812 bp
  90% of sequence in fragments shorter than     957 bp
  95% of sequence in fragments shorter than    1231 bp

# AluI.HhaI (AGCT+GCGC) double digest, 14783 thousand fragments (13027778+1756502 sites), 201 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     197 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     257 bp
  40% of sequence in fragments shorter than     289 bp
  45% of sequence in fragments shorter than     322 bp
  50% of sequence in fragments shorter than     355 bp
  55% of sequence in fragments shorter than     393 bp
  60% of sequence in fragments shorter than     436 bp
  65% of sequence in fragments shorter than     482 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     595 bp
  80% of sequence in fragments shorter than     672 bp
  85% of sequence in fragments shorter than     762 bp
  90% of sequence in fragments shorter than     898 bp
  95% of sequence in fragments shorter than    1125 bp

# HphI.TspRI (GGTGA+CASTG) double digest, 14772 thousand fragments (6007336+8765240 sites), 201 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     133 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     193 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     289 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     359 bp
  55% of sequence in fragments shorter than     399 bp
  60% of sequence in fragments shorter than     444 bp
  65% of sequence in fragments shorter than     494 bp
  70% of sequence in fragments shorter than     551 bp
  75% of sequence in fragments shorter than     617 bp
  80% of sequence in fragments shorter than     697 bp
  85% of sequence in fragments shorter than     799 bp
  90% of sequence in fragments shorter than     938 bp
  95% of sequence in fragments shorter than    1176 bp

# ApaI.DdeI (GGGCCC+CTNAG) double digest, 14771 thousand fragments (460339+14312045 sites), 201 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     247 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     311 bp
  50% of sequence in fragments shorter than     345 bp
  55% of sequence in fragments shorter than     381 bp
  60% of sequence in fragments shorter than     422 bp
  65% of sequence in fragments shorter than     468 bp
  70% of sequence in fragments shorter than     521 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     656 bp
  85% of sequence in fragments shorter than     750 bp
  90% of sequence in fragments shorter than     885 bp
  95% of sequence in fragments shorter than    1117 bp

# BthCI.HaeIII (GCNGC+GGCC) double digest, 14771 thousand fragments (6209929+8562230 sites), 201 bp average fragment length
   5% of sequence in fragments shorter than      59 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     140 bp
  20% of sequence in fragments shorter than     178 bp
  25% of sequence in fragments shorter than     220 bp
  30% of sequence in fragments shorter than     264 bp
  35% of sequence in fragments shorter than     307 bp
  40% of sequence in fragments shorter than     348 bp
  45% of sequence in fragments shorter than     398 bp
  50% of sequence in fragments shorter than     450 bp
  55% of sequence in fragments shorter than     510 bp
  60% of sequence in fragments shorter than     573 bp
  65% of sequence in fragments shorter than     648 bp
  70% of sequence in fragments shorter than     732 bp
  75% of sequence in fragments shorter than     832 bp
  80% of sequence in fragments shorter than     956 bp
  85% of sequence in fragments shorter than    1120 bp
  90% of sequence in fragments shorter than    1342 bp
  95% of sequence in fragments shorter than    1734 bp

# DdeI.EcoRV (CTNAG+GATATC) double digest, 14758 thousand fragments (14312045+446473 sites), 201 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     163 bp
  25% of sequence in fragments shorter than     186 bp
  30% of sequence in fragments shorter than     216 bp
  35% of sequence in fragments shorter than     247 bp
  40% of sequence in fragments shorter than     278 bp
  45% of sequence in fragments shorter than     309 bp
  50% of sequence in fragments shorter than     343 bp
  55% of sequence in fragments shorter than     378 bp
  60% of sequence in fragments shorter than     417 bp
  65% of sequence in fragments shorter than     462 bp
  70% of sequence in fragments shorter than     513 bp
  75% of sequence in fragments shorter than     570 bp
  80% of sequence in fragments shorter than     641 bp
  85% of sequence in fragments shorter than     732 bp
  90% of sequence in fragments shorter than     859 bp
  95% of sequence in fragments shorter than    1065 bp

# AvaIII.CviAII (ATGCAT+CATG) double digest, 14748 thousand fragments (933357+13815697 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     232 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     359 bp
  55% of sequence in fragments shorter than     398 bp
  60% of sequence in fragments shorter than     440 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     541 bp
  75% of sequence in fragments shorter than     602 bp
  80% of sequence in fragments shorter than     678 bp
  85% of sequence in fragments shorter than     776 bp
  90% of sequence in fragments shorter than     920 bp
  95% of sequence in fragments shorter than    1179 bp

# CviAII.StuI (CATG+AGGCCT) double digest, 14740 thousand fragments (13815697+925729 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     197 bp
  30% of sequence in fragments shorter than     227 bp
  35% of sequence in fragments shorter than     257 bp
  40% of sequence in fragments shorter than     287 bp
  45% of sequence in fragments shorter than     317 bp
  50% of sequence in fragments shorter than     349 bp
  55% of sequence in fragments shorter than     386 bp
  60% of sequence in fragments shorter than     426 bp
  65% of sequence in fragments shorter than     471 bp
  70% of sequence in fragments shorter than     522 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     651 bp
  85% of sequence in fragments shorter than     735 bp
  90% of sequence in fragments shorter than     860 bp
  95% of sequence in fragments shorter than    1061 bp

# AluI.BfiI (AGCT+ACTGGG) double digest, 14733 thousand fragments (13027778+1706594 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     196 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     251 bp
  40% of sequence in fragments shorter than     280 bp
  45% of sequence in fragments shorter than     311 bp
  50% of sequence in fragments shorter than     343 bp
  55% of sequence in fragments shorter than     377 bp
  60% of sequence in fragments shorter than     417 bp
  65% of sequence in fragments shorter than     461 bp
  70% of sequence in fragments shorter than     508 bp
  75% of sequence in fragments shorter than     566 bp
  80% of sequence in fragments shorter than     635 bp
  85% of sequence in fragments shorter than     722 bp
  90% of sequence in fragments shorter than     849 bp
  95% of sequence in fragments shorter than    1071 bp

# BccI.HaeIII (CCATC+GGCC) double digest, 14732 thousand fragments (6170927+8562230 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     205 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     280 bp
  40% of sequence in fragments shorter than     319 bp
  45% of sequence in fragments shorter than     355 bp
  50% of sequence in fragments shorter than     402 bp
  55% of sequence in fragments shorter than     451 bp
  60% of sequence in fragments shorter than     507 bp
  65% of sequence in fragments shorter than     569 bp
  70% of sequence in fragments shorter than     643 bp
  75% of sequence in fragments shorter than     727 bp
  80% of sequence in fragments shorter than     835 bp
  85% of sequence in fragments shorter than     965 bp
  90% of sequence in fragments shorter than    1151 bp
  95% of sequence in fragments shorter than    1476 bp

# CviAII.NdeI (CATG+CATATG) double digest, 14718 thousand fragments (13815697+903360 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     195 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     256 bp
  40% of sequence in fragments shorter than     287 bp
  45% of sequence in fragments shorter than     317 bp
  50% of sequence in fragments shorter than     352 bp
  55% of sequence in fragments shorter than     390 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     477 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     591 bp
  80% of sequence in fragments shorter than     666 bp
  85% of sequence in fragments shorter than     762 bp
  90% of sequence in fragments shorter than     906 bp
  95% of sequence in fragments shorter than    1162 bp

# DdeI.SpeI (CTNAG+ACTAGT) double digest, 14711 thousand fragments (14312045+400286 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     187 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     248 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     310 bp
  50% of sequence in fragments shorter than     343 bp
  55% of sequence in fragments shorter than     379 bp
  60% of sequence in fragments shorter than     418 bp
  65% of sequence in fragments shorter than     464 bp
  70% of sequence in fragments shorter than     515 bp
  75% of sequence in fragments shorter than     573 bp
  80% of sequence in fragments shorter than     646 bp
  85% of sequence in fragments shorter than     737 bp
  90% of sequence in fragments shorter than     867 bp
  95% of sequence in fragments shorter than    1093 bp

# DdeI.HpaI (CTNAG+GTTAAC) double digest, 14700 thousand fragments (14312045+389418 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     187 bp
  30% of sequence in fragments shorter than     217 bp
  35% of sequence in fragments shorter than     248 bp
  40% of sequence in fragments shorter than     279 bp
  45% of sequence in fragments shorter than     310 bp
  50% of sequence in fragments shorter than     344 bp
  55% of sequence in fragments shorter than     380 bp
  60% of sequence in fragments shorter than     419 bp
  65% of sequence in fragments shorter than     465 bp
  70% of sequence in fragments shorter than     517 bp
  75% of sequence in fragments shorter than     575 bp
  80% of sequence in fragments shorter than     648 bp
  85% of sequence in fragments shorter than     739 bp
  90% of sequence in fragments shorter than     871 bp
  95% of sequence in fragments shorter than    1097 bp

# DdeI.SapI (CTNAG+GCTCTTC) double digest, 14688 thousand fragments (14312045+377161 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     187 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     248 bp
  40% of sequence in fragments shorter than     280 bp
  45% of sequence in fragments shorter than     311 bp
  50% of sequence in fragments shorter than     345 bp
  55% of sequence in fragments shorter than     381 bp
  60% of sequence in fragments shorter than     421 bp
  65% of sequence in fragments shorter than     467 bp
  70% of sequence in fragments shorter than     518 bp
  75% of sequence in fragments shorter than     579 bp
  80% of sequence in fragments shorter than     653 bp
  85% of sequence in fragments shorter than     746 bp
  90% of sequence in fragments shorter than     879 bp
  95% of sequence in fragments shorter than    1109 bp

# DdeI.SmaI (CTNAG+CCCGGG) double digest, 14688 thousand fragments (14312045+376940 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     219 bp
  35% of sequence in fragments shorter than     251 bp
  40% of sequence in fragments shorter than     282 bp
  45% of sequence in fragments shorter than     315 bp
  50% of sequence in fragments shorter than     348 bp
  55% of sequence in fragments shorter than     386 bp
  60% of sequence in fragments shorter than     427 bp
  65% of sequence in fragments shorter than     473 bp
  70% of sequence in fragments shorter than     527 bp
  75% of sequence in fragments shorter than     586 bp
  80% of sequence in fragments shorter than     662 bp
  85% of sequence in fragments shorter than     757 bp
  90% of sequence in fragments shorter than     892 bp
  95% of sequence in fragments shorter than    1124 bp

# BsrI.Hpy188I (ACTGG+TCNGA) double digest, 14682 thousand fragments (5741310+8942153 sites), 202 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     188 bp
  30% of sequence in fragments shorter than     219 bp
  35% of sequence in fragments shorter than     249 bp
  40% of sequence in fragments shorter than     281 bp
  45% of sequence in fragments shorter than     314 bp
  50% of sequence in fragments shorter than     348 bp
  55% of sequence in fragments shorter than     388 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     476 bp
  70% of sequence in fragments shorter than     529 bp
  75% of sequence in fragments shorter than     591 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     754 bp
  90% of sequence in fragments shorter than     882 bp
  95% of sequence in fragments shorter than    1102 bp

# BamHI.DdeI (GGATCC+CTNAG) double digest, 14677 thousand fragments (365999+14312045 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     164 bp
  25% of sequence in fragments shorter than     187 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     249 bp
  40% of sequence in fragments shorter than     280 bp
  45% of sequence in fragments shorter than     312 bp
  50% of sequence in fragments shorter than     346 bp
  55% of sequence in fragments shorter than     383 bp
  60% of sequence in fragments shorter than     423 bp
  65% of sequence in fragments shorter than     469 bp
  70% of sequence in fragments shorter than     522 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     652 bp
  85% of sequence in fragments shorter than     747 bp
  90% of sequence in fragments shorter than     883 bp
  95% of sequence in fragments shorter than    1113 bp

# CviAII.HindIII (CATG+AAGCTT) double digest, 14675 thousand fragments (13815697+860365 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     196 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     316 bp
  50% of sequence in fragments shorter than     350 bp
  55% of sequence in fragments shorter than     387 bp
  60% of sequence in fragments shorter than     428 bp
  65% of sequence in fragments shorter than     473 bp
  70% of sequence in fragments shorter than     525 bp
  75% of sequence in fragments shorter than     583 bp
  80% of sequence in fragments shorter than     655 bp
  85% of sequence in fragments shorter than     749 bp
  90% of sequence in fragments shorter than     884 bp
  95% of sequence in fragments shorter than    1121 bp

# AluI.PsiI (AGCT+TTATAA) double digest, 14675 thousand fragments (13027778+1647912 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     196 bp
  30% of sequence in fragments shorter than     222 bp
  35% of sequence in fragments shorter than     250 bp
  40% of sequence in fragments shorter than     278 bp
  45% of sequence in fragments shorter than     308 bp
  50% of sequence in fragments shorter than     338 bp
  55% of sequence in fragments shorter than     371 bp
  60% of sequence in fragments shorter than     409 bp
  65% of sequence in fragments shorter than     450 bp
  70% of sequence in fragments shorter than     496 bp
  75% of sequence in fragments shorter than     549 bp
  80% of sequence in fragments shorter than     615 bp
  85% of sequence in fragments shorter than     699 bp
  90% of sequence in fragments shorter than     817 bp
  95% of sequence in fragments shorter than    1035 bp

# CviAII.XbaI (CATG+TCTAGA) double digest, 14666 thousand fragments (13815697+850998 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     197 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     317 bp
  50% of sequence in fragments shorter than     351 bp
  55% of sequence in fragments shorter than     388 bp
  60% of sequence in fragments shorter than     428 bp
  65% of sequence in fragments shorter than     473 bp
  70% of sequence in fragments shorter than     523 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     653 bp
  85% of sequence in fragments shorter than     743 bp
  90% of sequence in fragments shorter than     873 bp
  95% of sequence in fragments shorter than    1101 bp

# CviAII.EcoRI (CATG+GAATTC) double digest, 14662 thousand fragments (13815697+847401 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     198 bp
  30% of sequence in fragments shorter than     228 bp
  35% of sequence in fragments shorter than     257 bp
  40% of sequence in fragments shorter than     287 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     350 bp
  55% of sequence in fragments shorter than     386 bp
  60% of sequence in fragments shorter than     427 bp
  65% of sequence in fragments shorter than     472 bp
  70% of sequence in fragments shorter than     524 bp
  75% of sequence in fragments shorter than     583 bp
  80% of sequence in fragments shorter than     655 bp
  85% of sequence in fragments shorter than     740 bp
  90% of sequence in fragments shorter than     870 bp
  95% of sequence in fragments shorter than    1102 bp

# BsrI.HinfI (ACTGG+GANTC) double digest, 14656 thousand fragments (5741310+8916054 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     194 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     283 bp
  45% of sequence in fragments shorter than     314 bp
  50% of sequence in fragments shorter than     344 bp
  55% of sequence in fragments shorter than     376 bp
  60% of sequence in fragments shorter than     415 bp
  65% of sequence in fragments shorter than     458 bp
  70% of sequence in fragments shorter than     507 bp
  75% of sequence in fragments shorter than     563 bp
  80% of sequence in fragments shorter than     631 bp
  85% of sequence in fragments shorter than     715 bp
  90% of sequence in fragments shorter than     834 bp
  95% of sequence in fragments shorter than    1035 bp

# BstEII.DdeI (GGTNACC+CTNAG) double digest, 14647 thousand fragments (335865+14312045 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     188 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     249 bp
  40% of sequence in fragments shorter than     281 bp
  45% of sequence in fragments shorter than     313 bp
  50% of sequence in fragments shorter than     346 bp
  55% of sequence in fragments shorter than     383 bp
  60% of sequence in fragments shorter than     423 bp
  65% of sequence in fragments shorter than     469 bp
  70% of sequence in fragments shorter than     521 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     655 bp
  85% of sequence in fragments shorter than     748 bp
  90% of sequence in fragments shorter than     881 bp
  95% of sequence in fragments shorter than    1112 bp

# AluI.EsaBC3I (AGCT+TCGA) double digest, 14630 thousand fragments (13027778+1603346 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     196 bp
  30% of sequence in fragments shorter than     225 bp
  35% of sequence in fragments shorter than     254 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     316 bp
  50% of sequence in fragments shorter than     348 bp
  55% of sequence in fragments shorter than     384 bp
  60% of sequence in fragments shorter than     426 bp
  65% of sequence in fragments shorter than     469 bp
  70% of sequence in fragments shorter than     520 bp
  75% of sequence in fragments shorter than     579 bp
  80% of sequence in fragments shorter than     652 bp
  85% of sequence in fragments shorter than     742 bp
  90% of sequence in fragments shorter than     875 bp
  95% of sequence in fragments shorter than    1105 bp

# DdeI.SexAI (CTNAG+ACCWGGT) double digest, 14609 thousand fragments (14312045+297900 sites), 203 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     188 bp
  30% of sequence in fragments shorter than     218 bp
  35% of sequence in fragments shorter than     249 bp
  40% of sequence in fragments shorter than     281 bp
  45% of sequence in fragments shorter than     313 bp
  50% of sequence in fragments shorter than     346 bp
  55% of sequence in fragments shorter than     383 bp
  60% of sequence in fragments shorter than     424 bp
  65% of sequence in fragments shorter than     470 bp
  70% of sequence in fragments shorter than     523 bp
  75% of sequence in fragments shorter than     583 bp
  80% of sequence in fragments shorter than     657 bp
  85% of sequence in fragments shorter than     751 bp
  90% of sequence in fragments shorter than     885 bp
  95% of sequence in fragments shorter than    1116 bp

# DdeI.KpnI (CTNAG+GGTACC) double digest, 14599 thousand fragments (14312045+287974 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     219 bp
  35% of sequence in fragments shorter than     251 bp
  40% of sequence in fragments shorter than     282 bp
  45% of sequence in fragments shorter than     314 bp
  50% of sequence in fragments shorter than     347 bp
  55% of sequence in fragments shorter than     385 bp
  60% of sequence in fragments shorter than     425 bp
  65% of sequence in fragments shorter than     471 bp
  70% of sequence in fragments shorter than     522 bp
  75% of sequence in fragments shorter than     582 bp
  80% of sequence in fragments shorter than     657 bp
  85% of sequence in fragments shorter than     745 bp
  90% of sequence in fragments shorter than     873 bp
  95% of sequence in fragments shorter than    1093 bp

# DdeI.NheI (CTNAG+GCTAGC) double digest, 14598 thousand fragments (14312045+287384 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     219 bp
  35% of sequence in fragments shorter than     250 bp
  40% of sequence in fragments shorter than     282 bp
  45% of sequence in fragments shorter than     313 bp
  50% of sequence in fragments shorter than     347 bp
  55% of sequence in fragments shorter than     384 bp
  60% of sequence in fragments shorter than     424 bp
  65% of sequence in fragments shorter than     470 bp
  70% of sequence in fragments shorter than     523 bp
  75% of sequence in fragments shorter than     582 bp
  80% of sequence in fragments shorter than     656 bp
  85% of sequence in fragments shorter than     747 bp
  90% of sequence in fragments shorter than     881 bp
  95% of sequence in fragments shorter than    1096 bp

# BglII.CviAII (AGATCT+CATG) double digest, 14589 thousand fragments (774732+13815697 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     198 bp
  30% of sequence in fragments shorter than     228 bp
  35% of sequence in fragments shorter than     258 bp
  40% of sequence in fragments shorter than     288 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     391 bp
  60% of sequence in fragments shorter than     432 bp
  65% of sequence in fragments shorter than     478 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     590 bp
  80% of sequence in fragments shorter than     664 bp
  85% of sequence in fragments shorter than     759 bp
  90% of sequence in fragments shorter than     899 bp
  95% of sequence in fragments shorter than    1155 bp

# AluI.HgiCI (AGCT+GGYRCC) double digest, 14578 thousand fragments (13027778+1550877 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     140 bp
  20% of sequence in fragments shorter than     171 bp
  25% of sequence in fragments shorter than     200 bp
  30% of sequence in fragments shorter than     228 bp
  35% of sequence in fragments shorter than     259 bp
  40% of sequence in fragments shorter than     290 bp
  45% of sequence in fragments shorter than     322 bp
  50% of sequence in fragments shorter than     354 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     478 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     591 bp
  80% of sequence in fragments shorter than     667 bp
  85% of sequence in fragments shorter than     758 bp
  90% of sequence in fragments shorter than     895 bp
  95% of sequence in fragments shorter than    1141 bp

# AluI.BsmI (AGCT+GAATGC) double digest, 14576 thousand fragments (13027778+1549350 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     196 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     283 bp
  45% of sequence in fragments shorter than     315 bp
  50% of sequence in fragments shorter than     347 bp
  55% of sequence in fragments shorter than     383 bp
  60% of sequence in fragments shorter than     424 bp
  65% of sequence in fragments shorter than     467 bp
  70% of sequence in fragments shorter than     517 bp
  75% of sequence in fragments shorter than     575 bp
  80% of sequence in fragments shorter than     647 bp
  85% of sequence in fragments shorter than     736 bp
  90% of sequence in fragments shorter than     864 bp
  95% of sequence in fragments shorter than    1082 bp

# CviAII.NcoI (CATG+CCATGG) double digest, 14574 thousand fragments (13815697+759594 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     416 bp
  60% of sequence in fragments shorter than     460 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     629 bp
  80% of sequence in fragments shorter than     708 bp
  85% of sequence in fragments shorter than     812 bp
  90% of sequence in fragments shorter than     957 bp
  95% of sequence in fragments shorter than    1231 bp

# HaeIII.HphI (GGCC+GGTGA) double digest, 14569 thousand fragments (8562230+6007336 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      60 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     171 bp
  25% of sequence in fragments shorter than     207 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     282 bp
  40% of sequence in fragments shorter than     324 bp
  45% of sequence in fragments shorter than     358 bp
  50% of sequence in fragments shorter than     405 bp
  55% of sequence in fragments shorter than     454 bp
  60% of sequence in fragments shorter than     510 bp
  65% of sequence in fragments shorter than     571 bp
  70% of sequence in fragments shorter than     643 bp
  75% of sequence in fragments shorter than     726 bp
  80% of sequence in fragments shorter than     830 bp
  85% of sequence in fragments shorter than     955 bp
  90% of sequence in fragments shorter than    1144 bp
  95% of sequence in fragments shorter than    1457 bp

# BclI.CviAII (TGATCA+CATG) double digest, 14553 thousand fragments (738785+13815697 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     169 bp
  25% of sequence in fragments shorter than     200 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     261 bp
  40% of sequence in fragments shorter than     291 bp
  45% of sequence in fragments shorter than     323 bp
  50% of sequence in fragments shorter than     358 bp
  55% of sequence in fragments shorter than     396 bp
  60% of sequence in fragments shorter than     438 bp
  65% of sequence in fragments shorter than     485 bp
  70% of sequence in fragments shorter than     538 bp
  75% of sequence in fragments shorter than     600 bp
  80% of sequence in fragments shorter than     675 bp
  85% of sequence in fragments shorter than     773 bp
  90% of sequence in fragments shorter than     913 bp
  95% of sequence in fragments shorter than    1166 bp

# AvaII.ScrFI (GGWCC+CCNGG) double digest, 14552 thousand fragments (2747690+11805099 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      59 bp
  10% of sequence in fragments shorter than      92 bp
  15% of sequence in fragments shorter than     129 bp
  20% of sequence in fragments shorter than     169 bp
  25% of sequence in fragments shorter than     212 bp
  30% of sequence in fragments shorter than     260 bp
  35% of sequence in fragments shorter than     310 bp
  40% of sequence in fragments shorter than     360 bp
  45% of sequence in fragments shorter than     418 bp
  50% of sequence in fragments shorter than     484 bp
  55% of sequence in fragments shorter than     556 bp
  60% of sequence in fragments shorter than     636 bp
  65% of sequence in fragments shorter than     721 bp
  70% of sequence in fragments shorter than     829 bp
  75% of sequence in fragments shorter than     959 bp
  80% of sequence in fragments shorter than    1121 bp
  85% of sequence in fragments shorter than    1339 bp
  90% of sequence in fragments shorter than    1649 bp
  95% of sequence in fragments shorter than    2221 bp

# CviAII.FnuDII (CATG+CGCG) double digest, 14549 thousand fragments (13815697+733940 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     200 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     261 bp
  40% of sequence in fragments shorter than     292 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     359 bp
  55% of sequence in fragments shorter than     397 bp
  60% of sequence in fragments shorter than     439 bp
  65% of sequence in fragments shorter than     486 bp
  70% of sequence in fragments shorter than     540 bp
  75% of sequence in fragments shorter than     601 bp
  80% of sequence in fragments shorter than     676 bp
  85% of sequence in fragments shorter than     772 bp
  90% of sequence in fragments shorter than     912 bp
  95% of sequence in fragments shorter than    1158 bp

# BsrBI.DdeI (CCGCTC+CTNAG) double digest, 14547 thousand fragments (235523+14312045 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     220 bp
  35% of sequence in fragments shorter than     251 bp
  40% of sequence in fragments shorter than     283 bp
  45% of sequence in fragments shorter than     315 bp
  50% of sequence in fragments shorter than     348 bp
  55% of sequence in fragments shorter than     387 bp
  60% of sequence in fragments shorter than     427 bp
  65% of sequence in fragments shorter than     474 bp
  70% of sequence in fragments shorter than     527 bp
  75% of sequence in fragments shorter than     587 bp
  80% of sequence in fragments shorter than     662 bp
  85% of sequence in fragments shorter than     757 bp
  90% of sequence in fragments shorter than     892 bp
  95% of sequence in fragments shorter than    1124 bp

# DdeI.KasI (CTNAG+GGCGCC) double digest, 14541 thousand fragments (14312045+230381 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     221 bp
  35% of sequence in fragments shorter than     252 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     316 bp
  50% of sequence in fragments shorter than     349 bp
  55% of sequence in fragments shorter than     388 bp
  60% of sequence in fragments shorter than     429 bp
  65% of sequence in fragments shorter than     476 bp
  70% of sequence in fragments shorter than     529 bp
  75% of sequence in fragments shorter than     589 bp
  80% of sequence in fragments shorter than     665 bp
  85% of sequence in fragments shorter than     760 bp
  90% of sequence in fragments shorter than     895 bp
  95% of sequence in fragments shorter than    1127 bp

# DdeI.SwaI (CTNAG+ATTTAAAT) double digest, 14538 thousand fragments (14312045+226602 sites), 204 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     189 bp
  30% of sequence in fragments shorter than     220 bp
  35% of sequence in fragments shorter than     250 bp
  40% of sequence in fragments shorter than     282 bp
  45% of sequence in fragments shorter than     314 bp
  50% of sequence in fragments shorter than     347 bp
  55% of sequence in fragments shorter than     384 bp
  60% of sequence in fragments shorter than     424 bp
  65% of sequence in fragments shorter than     469 bp
  70% of sequence in fragments shorter than     522 bp
  75% of sequence in fragments shorter than     580 bp
  80% of sequence in fragments shorter than     654 bp
  85% of sequence in fragments shorter than     746 bp
  90% of sequence in fragments shorter than     878 bp
  95% of sequence in fragments shorter than    1105 bp

# ScrFI.SmlI (CCNGG+CTYRAG) double digest, 14532 thousand fragments (11805099+2727867 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      58 bp
  10% of sequence in fragments shorter than      91 bp
  15% of sequence in fragments shorter than     126 bp
  20% of sequence in fragments shorter than     165 bp
  25% of sequence in fragments shorter than     205 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     295 bp
  40% of sequence in fragments shorter than     343 bp
  45% of sequence in fragments shorter than     396 bp
  50% of sequence in fragments shorter than     455 bp
  55% of sequence in fragments shorter than     519 bp
  60% of sequence in fragments shorter than     591 bp
  65% of sequence in fragments shorter than     668 bp
  70% of sequence in fragments shorter than     761 bp
  75% of sequence in fragments shorter than     874 bp
  80% of sequence in fragments shorter than    1016 bp
  85% of sequence in fragments shorter than    1205 bp
  90% of sequence in fragments shorter than    1482 bp
  95% of sequence in fragments shorter than    2034 bp

# BsaAI.CviAII (YACGTR+CATG) double digest, 14528 thousand fragments (713801+13815697 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     200 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     260 bp
  40% of sequence in fragments shorter than     291 bp
  45% of sequence in fragments shorter than     322 bp
  50% of sequence in fragments shorter than     357 bp
  55% of sequence in fragments shorter than     396 bp
  60% of sequence in fragments shorter than     437 bp
  65% of sequence in fragments shorter than     484 bp
  70% of sequence in fragments shorter than     538 bp
  75% of sequence in fragments shorter than     598 bp
  80% of sequence in fragments shorter than     671 bp
  85% of sequence in fragments shorter than     766 bp
  90% of sequence in fragments shorter than     907 bp
  95% of sequence in fragments shorter than    1146 bp

# BceAI.CviAII (ACGGC+CATG) double digest, 14520 thousand fragments (705178+13815697 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     199 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     260 bp
  40% of sequence in fragments shorter than     290 bp
  45% of sequence in fragments shorter than     322 bp
  50% of sequence in fragments shorter than     357 bp
  55% of sequence in fragments shorter than     395 bp
  60% of sequence in fragments shorter than     436 bp
  65% of sequence in fragments shorter than     483 bp
  70% of sequence in fragments shorter than     536 bp
  75% of sequence in fragments shorter than     596 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     765 bp
  90% of sequence in fragments shorter than     904 bp
  95% of sequence in fragments shorter than    1142 bp

# AflIII.AluI (ACRYGT+AGCT) double digest, 14512 thousand fragments (1485394+13027778 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     140 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     198 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     282 bp
  45% of sequence in fragments shorter than     313 bp
  50% of sequence in fragments shorter than     344 bp
  55% of sequence in fragments shorter than     378 bp
  60% of sequence in fragments shorter than     417 bp
  65% of sequence in fragments shorter than     459 bp
  70% of sequence in fragments shorter than     507 bp
  75% of sequence in fragments shorter than     561 bp
  80% of sequence in fragments shorter than     629 bp
  85% of sequence in fragments shorter than     714 bp
  90% of sequence in fragments shorter than     836 bp
  95% of sequence in fragments shorter than    1051 bp

# BciVI.CviAII (GTATCC+CATG) double digest, 14511 thousand fragments (696093+13815697 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     169 bp
  25% of sequence in fragments shorter than     200 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     260 bp
  40% of sequence in fragments shorter than     290 bp
  45% of sequence in fragments shorter than     322 bp
  50% of sequence in fragments shorter than     357 bp
  55% of sequence in fragments shorter than     395 bp
  60% of sequence in fragments shorter than     436 bp
  65% of sequence in fragments shorter than     483 bp
  70% of sequence in fragments shorter than     537 bp
  75% of sequence in fragments shorter than     597 bp
  80% of sequence in fragments shorter than     672 bp
  85% of sequence in fragments shorter than     769 bp
  90% of sequence in fragments shorter than     912 bp
  95% of sequence in fragments shorter than    1164 bp

# BsrI.TspRI (ACTGG+CASTG) double digest, 14506 thousand fragments (5741310+8765240 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      79 bp
  10% of sequence in fragments shorter than     123 bp
  15% of sequence in fragments shorter than     159 bp
  20% of sequence in fragments shorter than     195 bp
  25% of sequence in fragments shorter than     224 bp
  30% of sequence in fragments shorter than     256 bp
  35% of sequence in fragments shorter than     291 bp
  40% of sequence in fragments shorter than     325 bp
  45% of sequence in fragments shorter than     361 bp
  50% of sequence in fragments shorter than     401 bp
  55% of sequence in fragments shorter than     443 bp
  60% of sequence in fragments shorter than     488 bp
  65% of sequence in fragments shorter than     539 bp
  70% of sequence in fragments shorter than     598 bp
  75% of sequence in fragments shorter than     666 bp
  80% of sequence in fragments shorter than     748 bp
  85% of sequence in fragments shorter than     855 bp
  90% of sequence in fragments shorter than    1010 bp
  95% of sequence in fragments shorter than    1288 bp

# BbvI.MboII (GCAGC+GAAGA) double digest, 14489 thousand fragments (5290051+9199496 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     267 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     418 bp
  60% of sequence in fragments shorter than     464 bp
  65% of sequence in fragments shorter than     516 bp
  70% of sequence in fragments shorter than     574 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     722 bp
  85% of sequence in fragments shorter than     826 bp
  90% of sequence in fragments shorter than     969 bp
  95% of sequence in fragments shorter than    1214 bp

# AclI.DdeI (AACGTT+CTNAG) double digest, 14484 thousand fragments (172706+14312045 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     166 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     221 bp
  35% of sequence in fragments shorter than     252 bp
  40% of sequence in fragments shorter than     283 bp
  45% of sequence in fragments shorter than     315 bp
  50% of sequence in fragments shorter than     348 bp
  55% of sequence in fragments shorter than     386 bp
  60% of sequence in fragments shorter than     427 bp
  65% of sequence in fragments shorter than     473 bp
  70% of sequence in fragments shorter than     526 bp
  75% of sequence in fragments shorter than     586 bp
  80% of sequence in fragments shorter than     659 bp
  85% of sequence in fragments shorter than     754 bp
  90% of sequence in fragments shorter than     888 bp
  95% of sequence in fragments shorter than    1120 bp

# BspKT6I.BspNCI (GATC+CCAGA) double digest, 14481 thousand fragments (7199434+7282436 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     194 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     256 bp
  40% of sequence in fragments shorter than     288 bp
  45% of sequence in fragments shorter than     320 bp
  50% of sequence in fragments shorter than     351 bp
  55% of sequence in fragments shorter than     389 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     476 bp
  70% of sequence in fragments shorter than     528 bp
  75% of sequence in fragments shorter than     589 bp
  80% of sequence in fragments shorter than     663 bp
  85% of sequence in fragments shorter than     751 bp
  90% of sequence in fragments shorter than     885 bp
  95% of sequence in fragments shorter than    1109 bp

# DdeI.PacI (CTNAG+TTAATTAA) double digest, 14470 thousand fragments (14312045+159455 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     221 bp
  35% of sequence in fragments shorter than     252 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     316 bp
  50% of sequence in fragments shorter than     348 bp
  55% of sequence in fragments shorter than     387 bp
  60% of sequence in fragments shorter than     427 bp
  65% of sequence in fragments shorter than     474 bp
  70% of sequence in fragments shorter than     527 bp
  75% of sequence in fragments shorter than     586 bp
  80% of sequence in fragments shorter than     660 bp
  85% of sequence in fragments shorter than     754 bp
  90% of sequence in fragments shorter than     888 bp
  95% of sequence in fragments shorter than    1118 bp

# DdeI.Hpy99I (CTNAG+CGWCG) double digest, 14469 thousand fragments (14312045+157917 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     190 bp
  30% of sequence in fragments shorter than     221 bp
  35% of sequence in fragments shorter than     252 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     316 bp
  50% of sequence in fragments shorter than     349 bp
  55% of sequence in fragments shorter than     388 bp
  60% of sequence in fragments shorter than     429 bp
  65% of sequence in fragments shorter than     475 bp
  70% of sequence in fragments shorter than     529 bp
  75% of sequence in fragments shorter than     589 bp
  80% of sequence in fragments shorter than     664 bp
  85% of sequence in fragments shorter than     758 bp
  90% of sequence in fragments shorter than     894 bp
  95% of sequence in fragments shorter than    1125 bp

# AluI.FauI (AGCT+CCCGC) double digest, 14467 thousand fragments (13027778+1439772 sites), 205 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     261 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     360 bp
  55% of sequence in fragments shorter than     398 bp
  60% of sequence in fragments shorter than     441 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     541 bp
  75% of sequence in fragments shorter than     604 bp
  80% of sequence in fragments shorter than     682 bp
  85% of sequence in fragments shorter than     776 bp
  90% of sequence in fragments shorter than     916 bp
  95% of sequence in fragments shorter than    1162 bp

# AluI.BspMI (AGCT+ACCTGC) double digest, 14465 thousand fragments (13027778+1438129 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     199 bp
  30% of sequence in fragments shorter than     226 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     285 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     349 bp
  55% of sequence in fragments shorter than     386 bp
  60% of sequence in fragments shorter than     428 bp
  65% of sequence in fragments shorter than     471 bp
  70% of sequence in fragments shorter than     523 bp
  75% of sequence in fragments shorter than     583 bp
  80% of sequence in fragments shorter than     656 bp
  85% of sequence in fragments shorter than     746 bp
  90% of sequence in fragments shorter than     878 bp
  95% of sequence in fragments shorter than    1107 bp

# AflII.CviAII (CTTAAG+CATG) double digest, 14458 thousand fragments (643284+13815697 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     200 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     260 bp
  40% of sequence in fragments shorter than     290 bp
  45% of sequence in fragments shorter than     322 bp
  50% of sequence in fragments shorter than     356 bp
  55% of sequence in fragments shorter than     395 bp
  60% of sequence in fragments shorter than     436 bp
  65% of sequence in fragments shorter than     483 bp
  70% of sequence in fragments shorter than     536 bp
  75% of sequence in fragments shorter than     598 bp
  80% of sequence in fragments shorter than     672 bp
  85% of sequence in fragments shorter than     769 bp
  90% of sequence in fragments shorter than     913 bp
  95% of sequence in fragments shorter than    1170 bp

# CviAII.HaeII (CATG+RGCGCY) double digest, 14445 thousand fragments (13815697+630101 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     231 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     292 bp
  45% of sequence in fragments shorter than     323 bp
  50% of sequence in fragments shorter than     356 bp
  55% of sequence in fragments shorter than     393 bp
  60% of sequence in fragments shorter than     434 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     531 bp
  75% of sequence in fragments shorter than     591 bp
  80% of sequence in fragments shorter than     663 bp
  85% of sequence in fragments shorter than     748 bp
  90% of sequence in fragments shorter than     873 bp
  95% of sequence in fragments shorter than    1075 bp

# DdeI.SnaBI (CTNAG+TACGTA) double digest, 14443 thousand fragments (14312045+132299 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     191 bp
  30% of sequence in fragments shorter than     221 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     284 bp
  45% of sequence in fragments shorter than     316 bp
  50% of sequence in fragments shorter than     349 bp
  55% of sequence in fragments shorter than     387 bp
  60% of sequence in fragments shorter than     428 bp
  65% of sequence in fragments shorter than     475 bp
  70% of sequence in fragments shorter than     528 bp
  75% of sequence in fragments shorter than     587 bp
  80% of sequence in fragments shorter than     662 bp
  85% of sequence in fragments shorter than     756 bp
  90% of sequence in fragments shorter than     890 bp
  95% of sequence in fragments shorter than    1120 bp

# DdeI.NaeI (CTNAG+GCCGGC) double digest, 14437 thousand fragments (14312045+125692 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     191 bp
  30% of sequence in fragments shorter than     222 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     285 bp
  45% of sequence in fragments shorter than     317 bp
  50% of sequence in fragments shorter than     350 bp
  55% of sequence in fragments shorter than     389 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     477 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     590 bp
  80% of sequence in fragments shorter than     666 bp
  85% of sequence in fragments shorter than     761 bp
  90% of sequence in fragments shorter than     897 bp
  95% of sequence in fragments shorter than    1128 bp

# DdeI.XhoI (CTNAG+CTCGAG) double digest, 14432 thousand fragments (14312045+121308 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     191 bp
  30% of sequence in fragments shorter than     222 bp
  35% of sequence in fragments shorter than     253 bp
  40% of sequence in fragments shorter than     285 bp
  45% of sequence in fragments shorter than     317 bp
  50% of sequence in fragments shorter than     350 bp
  55% of sequence in fragments shorter than     389 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     476 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     590 bp
  80% of sequence in fragments shorter than     666 bp
  85% of sequence in fragments shorter than     761 bp
  90% of sequence in fragments shorter than     896 bp
  95% of sequence in fragments shorter than    1128 bp

# CviAII.SacI (CATG+GAGCTC) double digest, 14418 thousand fragments (13815697+603323 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     169 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     232 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     360 bp
  55% of sequence in fragments shorter than     398 bp
  60% of sequence in fragments shorter than     440 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     541 bp
  75% of sequence in fragments shorter than     602 bp
  80% of sequence in fragments shorter than     677 bp
  85% of sequence in fragments shorter than     775 bp
  90% of sequence in fragments shorter than     918 bp
  95% of sequence in fragments shorter than    1168 bp

# AvrII.CviAII (CCTAGG+CATG) double digest, 14410 thousand fragments (594956+13815697 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     169 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     231 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     292 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     360 bp
  55% of sequence in fragments shorter than     399 bp
  60% of sequence in fragments shorter than     441 bp
  65% of sequence in fragments shorter than     488 bp
  70% of sequence in fragments shorter than     542 bp
  75% of sequence in fragments shorter than     605 bp
  80% of sequence in fragments shorter than     680 bp
  85% of sequence in fragments shorter than     779 bp
  90% of sequence in fragments shorter than     925 bp
  95% of sequence in fragments shorter than    1182 bp

# FokI.MboII (GGATG+GAAGA) double digest, 14400 thousand fragments (5201116+9199496 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     202 bp
  30% of sequence in fragments shorter than     233 bp
  35% of sequence in fragments shorter than     265 bp
  40% of sequence in fragments shorter than     299 bp
  45% of sequence in fragments shorter than     333 bp
  50% of sequence in fragments shorter than     367 bp
  55% of sequence in fragments shorter than     408 bp
  60% of sequence in fragments shorter than     450 bp
  65% of sequence in fragments shorter than     499 bp
  70% of sequence in fragments shorter than     552 bp
  75% of sequence in fragments shorter than     615 bp
  80% of sequence in fragments shorter than     691 bp
  85% of sequence in fragments shorter than     788 bp
  90% of sequence in fragments shorter than     920 bp
  95% of sequence in fragments shorter than    1146 bp

# ClaI.DdeI (ATCGAT+CTNAG) double digest, 14398 thousand fragments (86477+14312045 sites), 206 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     191 bp
  30% of sequence in fragments shorter than     222 bp
  35% of sequence in fragments shorter than     254 bp
  40% of sequence in fragments shorter than     285 bp
  45% of sequence in fragments shorter than     317 bp
  50% of sequence in fragments shorter than     351 bp
  55% of sequence in fragments shorter than     389 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     477 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     589 bp
  80% of sequence in fragments shorter than     664 bp
  85% of sequence in fragments shorter than     758 bp
  90% of sequence in fragments shorter than     892 bp
  95% of sequence in fragments shorter than    1121 bp

# CviAII.HgaI (CATG+GACGC) double digest, 14387 thousand fragments (13815697+571889 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     232 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     325 bp
  50% of sequence in fragments shorter than     360 bp
  55% of sequence in fragments shorter than     398 bp
  60% of sequence in fragments shorter than     440 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     540 bp
  75% of sequence in fragments shorter than     600 bp
  80% of sequence in fragments shorter than     674 bp
  85% of sequence in fragments shorter than     769 bp
  90% of sequence in fragments shorter than     906 bp
  95% of sequence in fragments shorter than    1148 bp

# CviAII.EciI (CATG+GGCGGA) double digest, 14386 thousand fragments (13815697+571273 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     169 bp
  25% of sequence in fragments shorter than     200 bp
  30% of sequence in fragments shorter than     232 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     325 bp
  50% of sequence in fragments shorter than     361 bp
  55% of sequence in fragments shorter than     400 bp
  60% of sequence in fragments shorter than     442 bp
  65% of sequence in fragments shorter than     490 bp
  70% of sequence in fragments shorter than     544 bp
  75% of sequence in fragments shorter than     606 bp
  80% of sequence in fragments shorter than     681 bp
  85% of sequence in fragments shorter than     778 bp
  90% of sequence in fragments shorter than     921 bp
  95% of sequence in fragments shorter than    1171 bp

# AatII.DdeI (GACGTC+CTNAG) double digest, 14382 thousand fragments (71089+14312045 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     254 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     351 bp
  55% of sequence in fragments shorter than     390 bp
  60% of sequence in fragments shorter than     431 bp
  65% of sequence in fragments shorter than     478 bp
  70% of sequence in fragments shorter than     532 bp
  75% of sequence in fragments shorter than     592 bp
  80% of sequence in fragments shorter than     668 bp
  85% of sequence in fragments shorter than     763 bp
  90% of sequence in fragments shorter than     899 bp
  95% of sequence in fragments shorter than    1130 bp

# CviAII.MfeI (CATG+CAATTG) double digest, 14379 thousand fragments (13815697+564303 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     169 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     231 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     292 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     359 bp
  55% of sequence in fragments shorter than     397 bp
  60% of sequence in fragments shorter than     439 bp
  65% of sequence in fragments shorter than     486 bp
  70% of sequence in fragments shorter than     539 bp
  75% of sequence in fragments shorter than     600 bp
  80% of sequence in fragments shorter than     675 bp
  85% of sequence in fragments shorter than     773 bp
  90% of sequence in fragments shorter than     917 bp
  95% of sequence in fragments shorter than    1167 bp

# DdeI.SacII (CTNAG+CCGCGG) double digest, 14378 thousand fragments (14312045+66901 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     254 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     351 bp
  55% of sequence in fragments shorter than     390 bp
  60% of sequence in fragments shorter than     431 bp
  65% of sequence in fragments shorter than     478 bp
  70% of sequence in fragments shorter than     532 bp
  75% of sequence in fragments shorter than     592 bp
  80% of sequence in fragments shorter than     667 bp
  85% of sequence in fragments shorter than     762 bp
  90% of sequence in fragments shorter than     898 bp
  95% of sequence in fragments shorter than    1130 bp

# DdeI.PspXI (CTNAG+VCTCGAGB) double digest, 14370 thousand fragments (14312045+59193 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     254 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     352 bp
  55% of sequence in fragments shorter than     391 bp
  60% of sequence in fragments shorter than     432 bp
  65% of sequence in fragments shorter than     479 bp
  70% of sequence in fragments shorter than     532 bp
  75% of sequence in fragments shorter than     593 bp
  80% of sequence in fragments shorter than     668 bp
  85% of sequence in fragments shorter than     763 bp
  90% of sequence in fragments shorter than     900 bp
  95% of sequence in fragments shorter than    1130 bp

# CviAII.SphI (CATG+GCATGC) double digest, 14366 thousand fragments (13815697+550952 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     416 bp
  60% of sequence in fragments shorter than     460 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     629 bp
  80% of sequence in fragments shorter than     708 bp
  85% of sequence in fragments shorter than     812 bp
  90% of sequence in fragments shorter than     957 bp
  95% of sequence in fragments shorter than    1231 bp

# AgeI.DdeI (ACCGGT+CTNAG) double digest, 14364 thousand fragments (53386+14312045 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     254 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     352 bp
  55% of sequence in fragments shorter than     390 bp
  60% of sequence in fragments shorter than     432 bp
  65% of sequence in fragments shorter than     479 bp
  70% of sequence in fragments shorter than     532 bp
  75% of sequence in fragments shorter than     592 bp
  80% of sequence in fragments shorter than     668 bp
  85% of sequence in fragments shorter than     763 bp
  90% of sequence in fragments shorter than     900 bp
  95% of sequence in fragments shorter than    1130 bp

# CviAII.ScaI (CATG+AGTACT) double digest, 14358 thousand fragments (13815697+543793 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     232 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     359 bp
  55% of sequence in fragments shorter than     398 bp
  60% of sequence in fragments shorter than     439 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     541 bp
  75% of sequence in fragments shorter than     602 bp
  80% of sequence in fragments shorter than     678 bp
  85% of sequence in fragments shorter than     777 bp
  90% of sequence in fragments shorter than     922 bp
  95% of sequence in fragments shorter than    1180 bp

# DdeI.PmeI (CTNAG+GTTTAAAC) double digest, 14353 thousand fragments (14312045+42094 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     254 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     318 bp
  50% of sequence in fragments shorter than     352 bp
  55% of sequence in fragments shorter than     391 bp
  60% of sequence in fragments shorter than     432 bp
  65% of sequence in fragments shorter than     479 bp
  70% of sequence in fragments shorter than     532 bp
  75% of sequence in fragments shorter than     592 bp
  80% of sequence in fragments shorter than     668 bp
  85% of sequence in fragments shorter than     763 bp
  90% of sequence in fragments shorter than     899 bp
  95% of sequence in fragments shorter than    1130 bp

# AluI.PstI (AGCT+CTGCAG) double digest, 14348 thousand fragments (13027778+1321469 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     206 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     264 bp
  40% of sequence in fragments shorter than     296 bp
  45% of sequence in fragments shorter than     328 bp
  50% of sequence in fragments shorter than     360 bp
  55% of sequence in fragments shorter than     398 bp
  60% of sequence in fragments shorter than     440 bp
  65% of sequence in fragments shorter than     485 bp
  70% of sequence in fragments shorter than     538 bp
  75% of sequence in fragments shorter than     599 bp
  80% of sequence in fragments shorter than     676 bp
  85% of sequence in fragments shorter than     769 bp
  90% of sequence in fragments shorter than     908 bp
  95% of sequence in fragments shorter than    1149 bp

# DdeI.SalI (CTNAG+GTCGAC) double digest, 14342 thousand fragments (14312045+31396 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     352 bp
  55% of sequence in fragments shorter than     391 bp
  60% of sequence in fragments shorter than     432 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     533 bp
  75% of sequence in fragments shorter than     593 bp
  80% of sequence in fragments shorter than     669 bp
  85% of sequence in fragments shorter than     765 bp
  90% of sequence in fragments shorter than     901 bp
  95% of sequence in fragments shorter than    1131 bp

# BspNCI.Hin4II (CCAGA+CCTTC) double digest, 14341 thousand fragments (7282436+7059915 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     314 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     391 bp
  55% of sequence in fragments shorter than     436 bp
  60% of sequence in fragments shorter than     485 bp
  65% of sequence in fragments shorter than     537 bp
  70% of sequence in fragments shorter than     598 bp
  75% of sequence in fragments shorter than     669 bp
  80% of sequence in fragments shorter than     753 bp
  85% of sequence in fragments shorter than     864 bp
  90% of sequence in fragments shorter than    1019 bp
  95% of sequence in fragments shorter than    1282 bp

# DdeI.MluI (CTNAG+ACGCGT) double digest, 14332 thousand fragments (14312045+21415 sites), 207 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     391 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     765 bp
  90% of sequence in fragments shorter than     901 bp
  95% of sequence in fragments shorter than    1132 bp

# DdeI.SgrAI (CTNAG+CRCCGGYG) double digest, 14326 thousand fragments (14312045+15270 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     765 bp
  90% of sequence in fragments shorter than     902 bp
  95% of sequence in fragments shorter than    1132 bp

# DdeI.NruI (CTNAG+TCGCGA) double digest, 14326 thousand fragments (14312045+14872 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     766 bp
  90% of sequence in fragments shorter than     902 bp
  95% of sequence in fragments shorter than    1132 bp

# DdeI.PvuI (CTNAG+CGATCG) double digest, 14325 thousand fragments (14312045+14186 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     766 bp
  90% of sequence in fragments shorter than     902 bp
  95% of sequence in fragments shorter than    1132 bp

# DdeI.FseI (CTNAG+GGCCGGCC) double digest, 14325 thousand fragments (14312045+13662 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     223 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     286 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     765 bp
  90% of sequence in fragments shorter than     902 bp
  95% of sequence in fragments shorter than    1132 bp

# DdeI.NotI (CTNAG+GCGGCCGC) double digest, 14321 thousand fragments (14312045+9795 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     287 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     765 bp
  90% of sequence in fragments shorter than     902 bp
  95% of sequence in fragments shorter than    1132 bp

# DdeI.RsrII (CTNAG+CGGWCCG) double digest, 14321 thousand fragments (14312045+9764 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     192 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     287 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     766 bp
  90% of sequence in fragments shorter than     902 bp
  95% of sequence in fragments shorter than    1132 bp

# AscI.DdeI (GGCGCGCC+CTNAG) double digest, 14316 thousand fragments (4751+14312045 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     168 bp
  25% of sequence in fragments shorter than     193 bp
  30% of sequence in fragments shorter than     224 bp
  35% of sequence in fragments shorter than     255 bp
  40% of sequence in fragments shorter than     287 bp
  45% of sequence in fragments shorter than     319 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     433 bp
  65% of sequence in fragments shorter than     480 bp
  70% of sequence in fragments shorter than     534 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     670 bp
  85% of sequence in fragments shorter than     766 bp
  90% of sequence in fragments shorter than     902 bp
  95% of sequence in fragments shorter than    1132 bp

# CviQI.MboII (GTAC+GAAGA) double digest, 14314 thousand fragments (5115078+9199496 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     199 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     294 bp
  45% of sequence in fragments shorter than     329 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     404 bp
  60% of sequence in fragments shorter than     448 bp
  65% of sequence in fragments shorter than     496 bp
  70% of sequence in fragments shorter than     548 bp
  75% of sequence in fragments shorter than     610 bp
  80% of sequence in fragments shorter than     685 bp
  85% of sequence in fragments shorter than     776 bp
  90% of sequence in fragments shorter than     907 bp
  95% of sequence in fragments shorter than    1133 bp

# ApaLI.CviAII (GTGCAC+CATG) double digest, 14311 thousand fragments (496255+13815697 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     140 bp
  20% of sequence in fragments shorter than     171 bp
  25% of sequence in fragments shorter than     203 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     265 bp
  40% of sequence in fragments shorter than     296 bp
  45% of sequence in fragments shorter than     328 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     403 bp
  60% of sequence in fragments shorter than     446 bp
  65% of sequence in fragments shorter than     494 bp
  70% of sequence in fragments shorter than     549 bp
  75% of sequence in fragments shorter than     611 bp
  80% of sequence in fragments shorter than     688 bp
  85% of sequence in fragments shorter than     788 bp
  90% of sequence in fragments shorter than     934 bp
  95% of sequence in fragments shorter than    1191 bp

# BsrI.HaeIII (ACTGG+GGCC) double digest, 14303 thousand fragments (5741310+8562230 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     178 bp
  25% of sequence in fragments shorter than     215 bp
  30% of sequence in fragments shorter than     251 bp
  35% of sequence in fragments shorter than     290 bp
  40% of sequence in fragments shorter than     329 bp
  45% of sequence in fragments shorter than     364 bp
  50% of sequence in fragments shorter than     409 bp
  55% of sequence in fragments shorter than     456 bp
  60% of sequence in fragments shorter than     507 bp
  65% of sequence in fragments shorter than     565 bp
  70% of sequence in fragments shorter than     634 bp
  75% of sequence in fragments shorter than     711 bp
  80% of sequence in fragments shorter than     808 bp
  85% of sequence in fragments shorter than     932 bp
  90% of sequence in fragments shorter than    1106 bp
  95% of sequence in fragments shorter than    1409 bp

# ApaI.CviAII (GGGCCC+CATG) double digest, 14275 thousand fragments (460339+13815697 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     140 bp
  20% of sequence in fragments shorter than     171 bp
  25% of sequence in fragments shorter than     203 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     264 bp
  40% of sequence in fragments shorter than     295 bp
  45% of sequence in fragments shorter than     327 bp
  50% of sequence in fragments shorter than     363 bp
  55% of sequence in fragments shorter than     402 bp
  60% of sequence in fragments shorter than     444 bp
  65% of sequence in fragments shorter than     492 bp
  70% of sequence in fragments shorter than     547 bp
  75% of sequence in fragments shorter than     609 bp
  80% of sequence in fragments shorter than     685 bp
  85% of sequence in fragments shorter than     784 bp
  90% of sequence in fragments shorter than     929 bp
  95% of sequence in fragments shorter than    1181 bp

# CviAII.EcoRV (CATG+GATATC) double digest, 14261 thousand fragments (13815697+446473 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     140 bp
  20% of sequence in fragments shorter than     171 bp
  25% of sequence in fragments shorter than     203 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     264 bp
  40% of sequence in fragments shorter than     295 bp
  45% of sequence in fragments shorter than     327 bp
  50% of sequence in fragments shorter than     362 bp
  55% of sequence in fragments shorter than     401 bp
  60% of sequence in fragments shorter than     443 bp
  65% of sequence in fragments shorter than     489 bp
  70% of sequence in fragments shorter than     542 bp
  75% of sequence in fragments shorter than     602 bp
  80% of sequence in fragments shorter than     676 bp
  85% of sequence in fragments shorter than     773 bp
  90% of sequence in fragments shorter than     914 bp
  95% of sequence in fragments shorter than    1171 bp

# BspKT6I.Hin4II (GATC+CCTTC) double digest, 14258 thousand fragments (7199434+7059915 sites), 208 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     173 bp
  25% of sequence in fragments shorter than     197 bp
  30% of sequence in fragments shorter than     228 bp
  35% of sequence in fragments shorter than     260 bp
  40% of sequence in fragments shorter than     291 bp
  45% of sequence in fragments shorter than     324 bp
  50% of sequence in fragments shorter than     357 bp
  55% of sequence in fragments shorter than     394 bp
  60% of sequence in fragments shorter than     435 bp
  65% of sequence in fragments shorter than     481 bp
  70% of sequence in fragments shorter than     533 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     668 bp
  85% of sequence in fragments shorter than     762 bp
  90% of sequence in fragments shorter than     895 bp
  95% of sequence in fragments shorter than    1115 bp

# AluI.BscGI (AGCT+CCCGT) double digest, 14258 thousand fragments (13027778+1231070 sites), 209 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     173 bp
  25% of sequence in fragments shorter than     203 bp
  30% of sequence in fragments shorter than     232 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     325 bp
  50% of sequence in fragments shorter than     356 bp
  55% of sequence in fragments shorter than     394 bp
  60% of sequence in fragments shorter than     436 bp
  65% of sequence in fragments shorter than     481 bp
  70% of sequence in fragments shorter than     533 bp
  75% of sequence in fragments shorter than     594 bp
  80% of sequence in fragments shorter than     668 bp
  85% of sequence in fragments shorter than     758 bp
  90% of sequence in fragments shorter than     891 bp
  95% of sequence in fragments shorter than    1120 bp

# BbvI.Hpy188I (GCAGC+TCNGA) double digest, 14231 thousand fragments (5290051+8942153 sites), 209 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     173 bp
  25% of sequence in fragments shorter than     202 bp
  30% of sequence in fragments shorter than     236 bp
  35% of sequence in fragments shorter than     267 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     340 bp
  50% of sequence in fragments shorter than     377 bp
  55% of sequence in fragments shorter than     420 bp
  60% of sequence in fragments shorter than     467 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     580 bp
  75% of sequence in fragments shorter than     649 bp
  80% of sequence in fragments shorter than     734 bp
  85% of sequence in fragments shorter than     842 bp
  90% of sequence in fragments shorter than     993 bp
  95% of sequence in fragments shorter than    1234 bp

# CviAII.SpeI (CATG+ACTAGT) double digest, 14215 thousand fragments (13815697+400286 sites), 209 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     140 bp
  20% of sequence in fragments shorter than     171 bp
  25% of sequence in fragments shorter than     203 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     264 bp
  40% of sequence in fragments shorter than     296 bp
  45% of sequence in fragments shorter than     328 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     403 bp
  60% of sequence in fragments shorter than     445 bp
  65% of sequence in fragments shorter than     493 bp
  70% of sequence in fragments shorter than     547 bp
  75% of sequence in fragments shorter than     610 bp
  80% of sequence in fragments shorter than     686 bp
  85% of sequence in fragments shorter than     786 bp
  90% of sequence in fragments shorter than     932 bp
  95% of sequence in fragments shorter than    1189 bp

# BbvI.HinfI (GCAGC+GANTC) double digest, 14205 thousand fragments (5290051+8916054 sites), 209 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     206 bp
  30% of sequence in fragments shorter than     237 bp
  35% of sequence in fragments shorter than     269 bp
  40% of sequence in fragments shorter than     302 bp
  45% of sequence in fragments shorter than     335 bp
  50% of sequence in fragments shorter than     368 bp
  55% of sequence in fragments shorter than     407 bp
  60% of sequence in fragments shorter than     451 bp
  65% of sequence in fragments shorter than     499 bp
  70% of sequence in fragments shorter than     555 bp
  75% of sequence in fragments shorter than     620 bp
  80% of sequence in fragments shorter than     694 bp
  85% of sequence in fragments shorter than     791 bp
  90% of sequence in fragments shorter than     928 bp
  95% of sequence in fragments shorter than    1163 bp

# CviAII.HpaI (CATG+GTTAAC) double digest, 14204 thousand fragments (13815697+389418 sites), 209 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     204 bp
  30% of sequence in fragments shorter than     235 bp
  35% of sequence in fragments shorter than     265 bp
  40% of sequence in fragments shorter than     296 bp
  45% of sequence in fragments shorter than     328 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     403 bp
  60% of sequence in fragments shorter than     445 bp
  65% of sequence in fragments shorter than     493 bp
  70% of sequence in fragments shorter than     548 bp
  75% of sequence in fragments shorter than     610 bp
  80% of sequence in fragments shorter than     686 bp
  85% of sequence in fragments shorter than     786 bp
  90% of sequence in fragments shorter than     934 bp
  95% of sequence in fragments shorter than    1194 bp

# CviAII.SapI (CATG+GCTCTTC) double digest, 14192 thousand fragments (13815697+377161 sites), 209 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     204 bp
  30% of sequence in fragments shorter than     235 bp
  35% of sequence in fragments shorter than     266 bp
  40% of sequence in fragments shorter than     297 bp
  45% of sequence in fragments shorter than     329 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     402 bp
  60% of sequence in fragments shorter than     444 bp
  65% of sequence in fragments shorter than     492 bp
  70% of sequence in fragments shorter than     546 bp
  75% of sequence in fragments shorter than     608 bp
  80% of sequence in fragments shorter than     686 bp
  85% of sequence in fragments shorter than     784 bp
  90% of sequence in fragments shorter than     931 bp
  95% of sequence in fragments shorter than    1186 bp

# CviAII.SmaI (CATG+CCCGGG) double digest, 14192 thousand fragments (13815697+376940 sites), 209 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     140 bp
  20% of sequence in fragments shorter than     171 bp
  25% of sequence in fragments shorter than     203 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     265 bp
  40% of sequence in fragments shorter than     296 bp
  45% of sequence in fragments shorter than     328 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     403 bp
  60% of sequence in fragments shorter than     446 bp
  65% of sequence in fragments shorter than     494 bp
  70% of sequence in fragments shorter than     548 bp
  75% of sequence in fragments shorter than     610 bp
  80% of sequence in fragments shorter than     686 bp
  85% of sequence in fragments shorter than     785 bp
  90% of sequence in fragments shorter than     930 bp
  95% of sequence in fragments shorter than    1181 bp

# MboII.TfiI (GAAGA+GAWTC) double digest, 14183 thousand fragments (9199496+4984362 sites), 210 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     114 bp
  15% of sequence in fragments shorter than     146 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     208 bp
  30% of sequence in fragments shorter than     237 bp
  35% of sequence in fragments shorter than     270 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     376 bp
  55% of sequence in fragments shorter than     417 bp
  60% of sequence in fragments shorter than     462 bp
  65% of sequence in fragments shorter than     511 bp
  70% of sequence in fragments shorter than     567 bp
  75% of sequence in fragments shorter than     631 bp
  80% of sequence in fragments shorter than     705 bp
  85% of sequence in fragments shorter than     803 bp
  90% of sequence in fragments shorter than     937 bp
  95% of sequence in fragments shorter than    1167 bp

# ScrFI.SspI (CCNGG+AATATT) double digest, 14181 thousand fragments (11805099+2377267 sites), 210 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     130 bp
  20% of sequence in fragments shorter than     167 bp
  25% of sequence in fragments shorter than     204 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     285 bp
  40% of sequence in fragments shorter than     328 bp
  45% of sequence in fragments shorter than     373 bp
  50% of sequence in fragments shorter than     423 bp
  55% of sequence in fragments shorter than     476 bp
  60% of sequence in fragments shorter than     535 bp
  65% of sequence in fragments shorter than     600 bp
  70% of sequence in fragments shorter than     677 bp
  75% of sequence in fragments shorter than     764 bp
  80% of sequence in fragments shorter than     877 bp
  85% of sequence in fragments shorter than    1026 bp
  90% of sequence in fragments shorter than    1256 bp
  95% of sequence in fragments shorter than    1707 bp

# BamHI.CviAII (GGATCC+CATG) double digest, 14181 thousand fragments (365999+13815697 sites), 210 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     204 bp
  30% of sequence in fragments shorter than     235 bp
  35% of sequence in fragments shorter than     266 bp
  40% of sequence in fragments shorter than     297 bp
  45% of sequence in fragments shorter than     330 bp
  50% of sequence in fragments shorter than     366 bp
  55% of sequence in fragments shorter than     405 bp
  60% of sequence in fragments shorter than     448 bp
  65% of sequence in fragments shorter than     496 bp
  70% of sequence in fragments shorter than     551 bp
  75% of sequence in fragments shorter than     613 bp
  80% of sequence in fragments shorter than     689 bp
  85% of sequence in fragments shorter than     789 bp
  90% of sequence in fragments shorter than     934 bp
  95% of sequence in fragments shorter than    1184 bp

# BstEII.CviAII (GGTNACC+CATG) double digest, 14151 thousand fragments (335865+13815697 sites), 210 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     173 bp
  25% of sequence in fragments shorter than     205 bp
  30% of sequence in fragments shorter than     236 bp
  35% of sequence in fragments shorter than     267 bp
  40% of sequence in fragments shorter than     297 bp
  45% of sequence in fragments shorter than     330 bp
  50% of sequence in fragments shorter than     366 bp
  55% of sequence in fragments shorter than     405 bp
  60% of sequence in fragments shorter than     447 bp
  65% of sequence in fragments shorter than     496 bp
  70% of sequence in fragments shorter than     550 bp
  75% of sequence in fragments shorter than     613 bp
  80% of sequence in fragments shorter than     690 bp
  85% of sequence in fragments shorter than     790 bp
  90% of sequence in fragments shorter than     937 bp
  95% of sequence in fragments shorter than    1195 bp

# FokI.Hpy188I (GGATG+TCNGA) double digest, 14142 thousand fragments (5201116+8942153 sites), 210 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     200 bp
  30% of sequence in fragments shorter than     230 bp
  35% of sequence in fragments shorter than     261 bp
  40% of sequence in fragments shorter than     295 bp
  45% of sequence in fragments shorter than     329 bp
  50% of sequence in fragments shorter than     361 bp
  55% of sequence in fragments shorter than     400 bp
  60% of sequence in fragments shorter than     444 bp
  65% of sequence in fragments shorter than     491 bp
  70% of sequence in fragments shorter than     543 bp
  75% of sequence in fragments shorter than     605 bp
  80% of sequence in fragments shorter than     681 bp
  85% of sequence in fragments shorter than     776 bp
  90% of sequence in fragments shorter than     907 bp
  95% of sequence in fragments shorter than    1130 bp

# AccI.AluI (GTMKAC+AGCT) double digest, 14128 thousand fragments (1101063+13027778 sites), 210 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     204 bp
  30% of sequence in fragments shorter than     231 bp
  35% of sequence in fragments shorter than     260 bp
  40% of sequence in fragments shorter than     290 bp
  45% of sequence in fragments shorter than     322 bp
  50% of sequence in fragments shorter than     353 bp
  55% of sequence in fragments shorter than     390 bp
  60% of sequence in fragments shorter than     430 bp
  65% of sequence in fragments shorter than     473 bp
  70% of sequence in fragments shorter than     523 bp
  75% of sequence in fragments shorter than     581 bp
  80% of sequence in fragments shorter than     652 bp
  85% of sequence in fragments shorter than     739 bp
  90% of sequence in fragments shorter than     866 bp
  95% of sequence in fragments shorter than    1083 bp

# HpaII.ScrFI (CCGG+CCNGG) double digest, 14122 thousand fragments (2317722+11805099 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     194 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     303 bp
  35% of sequence in fragments shorter than     363 bp
  40% of sequence in fragments shorter than     429 bp
  45% of sequence in fragments shorter than     501 bp
  50% of sequence in fragments shorter than     582 bp
  55% of sequence in fragments shorter than     666 bp
  60% of sequence in fragments shorter than     766 bp
  65% of sequence in fragments shorter than     882 bp
  70% of sequence in fragments shorter than    1017 bp
  75% of sequence in fragments shorter than    1183 bp
  80% of sequence in fragments shorter than    1389 bp
  85% of sequence in fragments shorter than    1668 bp
  90% of sequence in fragments shorter than    2066 bp
  95% of sequence in fragments shorter than    2832 bp

# FokI.HinfI (GGATG+GANTC) double digest, 14116 thousand fragments (5201116+8916054 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     231 bp
  35% of sequence in fragments shorter than     262 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     355 bp
  55% of sequence in fragments shorter than     392 bp
  60% of sequence in fragments shorter than     434 bp
  65% of sequence in fragments shorter than     479 bp
  70% of sequence in fragments shorter than     529 bp
  75% of sequence in fragments shorter than     589 bp
  80% of sequence in fragments shorter than     660 bp
  85% of sequence in fragments shorter than     749 bp
  90% of sequence in fragments shorter than     875 bp
  95% of sequence in fragments shorter than    1089 bp

# CviAII.SexAI (CATG+ACCWGGT) double digest, 14113 thousand fragments (13815697+297900 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     173 bp
  25% of sequence in fragments shorter than     205 bp
  30% of sequence in fragments shorter than     236 bp
  35% of sequence in fragments shorter than     267 bp
  40% of sequence in fragments shorter than     298 bp
  45% of sequence in fragments shorter than     331 bp
  50% of sequence in fragments shorter than     367 bp
  55% of sequence in fragments shorter than     406 bp
  60% of sequence in fragments shorter than     450 bp
  65% of sequence in fragments shorter than     498 bp
  70% of sequence in fragments shorter than     553 bp
  75% of sequence in fragments shorter than     616 bp
  80% of sequence in fragments shorter than     692 bp
  85% of sequence in fragments shorter than     794 bp
  90% of sequence in fragments shorter than     941 bp
  95% of sequence in fragments shorter than    1203 bp

# AluI.PpuMI (AGCT+RGGWCCY) double digest, 14112 thousand fragments (13027778+1085138 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     204 bp
  30% of sequence in fragments shorter than     233 bp
  35% of sequence in fragments shorter than     263 bp
  40% of sequence in fragments shorter than     294 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     360 bp
  55% of sequence in fragments shorter than     397 bp
  60% of sequence in fragments shorter than     439 bp
  65% of sequence in fragments shorter than     485 bp
  70% of sequence in fragments shorter than     537 bp
  75% of sequence in fragments shorter than     598 bp
  80% of sequence in fragments shorter than     676 bp
  85% of sequence in fragments shorter than     767 bp
  90% of sequence in fragments shorter than     906 bp
  95% of sequence in fragments shorter than    1153 bp

# AluI.PvuII (AGCT+CAGCTG) double digest, 14111 thousand fragments (13027778+1084260 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     318 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     577 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     820 bp
  90% of sequence in fragments shorter than     966 bp
  95% of sequence in fragments shorter than    1226 bp

# CviAII.KpnI (CATG+GGTACC) double digest, 14103 thousand fragments (13815697+287974 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     206 bp
  30% of sequence in fragments shorter than     237 bp
  35% of sequence in fragments shorter than     268 bp
  40% of sequence in fragments shorter than     299 bp
  45% of sequence in fragments shorter than     331 bp
  50% of sequence in fragments shorter than     367 bp
  55% of sequence in fragments shorter than     407 bp
  60% of sequence in fragments shorter than     450 bp
  65% of sequence in fragments shorter than     498 bp
  70% of sequence in fragments shorter than     553 bp
  75% of sequence in fragments shorter than     615 bp
  80% of sequence in fragments shorter than     692 bp
  85% of sequence in fragments shorter than     789 bp
  90% of sequence in fragments shorter than     934 bp
  95% of sequence in fragments shorter than    1202 bp

# CviAII.NheI (CATG+GCTAGC) double digest, 14102 thousand fragments (13815697+287384 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     205 bp
  30% of sequence in fragments shorter than     236 bp
  35% of sequence in fragments shorter than     267 bp
  40% of sequence in fragments shorter than     298 bp
  45% of sequence in fragments shorter than     331 bp
  50% of sequence in fragments shorter than     367 bp
  55% of sequence in fragments shorter than     407 bp
  60% of sequence in fragments shorter than     449 bp
  65% of sequence in fragments shorter than     498 bp
  70% of sequence in fragments shorter than     553 bp
  75% of sequence in fragments shorter than     614 bp
  80% of sequence in fragments shorter than     689 bp
  85% of sequence in fragments shorter than     787 bp
  90% of sequence in fragments shorter than     930 bp
  95% of sequence in fragments shorter than    1191 bp

# AciI.BstNI (CCGC+CCWGG) double digest, 14061 thousand fragments (4206130+9855646 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      56 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     225 bp
  30% of sequence in fragments shorter than     278 bp
  35% of sequence in fragments shorter than     332 bp
  40% of sequence in fragments shorter than     391 bp
  45% of sequence in fragments shorter than     457 bp
  50% of sequence in fragments shorter than     530 bp
  55% of sequence in fragments shorter than     608 bp
  60% of sequence in fragments shorter than     695 bp
  65% of sequence in fragments shorter than     796 bp
  70% of sequence in fragments shorter than     913 bp
  75% of sequence in fragments shorter than    1051 bp
  80% of sequence in fragments shorter than    1228 bp
  85% of sequence in fragments shorter than    1449 bp
  90% of sequence in fragments shorter than    1774 bp
  95% of sequence in fragments shorter than    2334 bp

# CviQI.Hpy188I (GTAC+TCNGA) double digest, 14056 thousand fragments (5115078+8942153 sites), 211 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     172 bp
  25% of sequence in fragments shorter than     199 bp
  30% of sequence in fragments shorter than     229 bp
  35% of sequence in fragments shorter than     260 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     327 bp
  50% of sequence in fragments shorter than     360 bp
  55% of sequence in fragments shorter than     400 bp
  60% of sequence in fragments shorter than     443 bp
  65% of sequence in fragments shorter than     488 bp
  70% of sequence in fragments shorter than     542 bp
  75% of sequence in fragments shorter than     603 bp
  80% of sequence in fragments shorter than     677 bp
  85% of sequence in fragments shorter than     765 bp
  90% of sequence in fragments shorter than     893 bp
  95% of sequence in fragments shorter than    1112 bp

# BbvI.TspRI (GCAGC+CASTG) double digest, 14054 thousand fragments (5290051+8765240 sites), 212 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     186 bp
  25% of sequence in fragments shorter than     216 bp
  30% of sequence in fragments shorter than     248 bp
  35% of sequence in fragments shorter than     283 bp
  40% of sequence in fragments shorter than     320 bp
  45% of sequence in fragments shorter than     357 bp
  50% of sequence in fragments shorter than     400 bp
  55% of sequence in fragments shorter than     446 bp
  60% of sequence in fragments shorter than     496 bp
  65% of sequence in fragments shorter than     553 bp
  70% of sequence in fragments shorter than     618 bp
  75% of sequence in fragments shorter than     694 bp
  80% of sequence in fragments shorter than     783 bp
  85% of sequence in fragments shorter than     895 bp
  90% of sequence in fragments shorter than    1050 bp
  95% of sequence in fragments shorter than    1334 bp

# BsrBI.CviAII (CCGCTC+CATG) double digest, 14050 thousand fragments (235523+13815697 sites), 212 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     206 bp
  30% of sequence in fragments shorter than     238 bp
  35% of sequence in fragments shorter than     269 bp
  40% of sequence in fragments shorter than     300 bp
  45% of sequence in fragments shorter than     332 bp
  50% of sequence in fragments shorter than     369 bp
  55% of sequence in fragments shorter than     408 bp
  60% of sequence in fragments shorter than     451 bp
  65% of sequence in fragments shorter than     499 bp
  70% of sequence in fragments shorter than     554 bp
  75% of sequence in fragments shorter than     616 bp
  80% of sequence in fragments shorter than     692 bp
  85% of sequence in fragments shorter than     791 bp
  90% of sequence in fragments shorter than     935 bp
  95% of sequence in fragments shorter than    1184 bp

# CviAII.KasI (CATG+GGCGCC) double digest, 14045 thousand fragments (13815697+230381 sites), 212 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     207 bp
  30% of sequence in fragments shorter than     238 bp
  35% of sequence in fragments shorter than     269 bp
  40% of sequence in fragments shorter than     300 bp
  45% of sequence in fragments shorter than     332 bp
  50% of sequence in fragments shorter than     368 bp
  55% of sequence in fragments shorter than     408 bp
  60% of sequence in fragments shorter than     451 bp
  65% of sequence in fragments shorter than     499 bp
  70% of sequence in fragments shorter than     554 bp
  75% of sequence in fragments shorter than     616 bp
  80% of sequence in fragments shorter than     692 bp
  85% of sequence in fragments shorter than     792 bp
  90% of sequence in fragments shorter than     936 bp
  95% of sequence in fragments shorter than    1187 bp

# CviAII.SwaI (CATG+ATTTAAAT) double digest, 14041 thousand fragments (13815697+226602 sites), 212 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     206 bp
  30% of sequence in fragments shorter than     237 bp
  35% of sequence in fragments shorter than     268 bp
  40% of sequence in fragments shorter than     299 bp
  45% of sequence in fragments shorter than     332 bp
  50% of sequence in fragments shorter than     368 bp
  55% of sequence in fragments shorter than     407 bp
  60% of sequence in fragments shorter than     450 bp
  65% of sequence in fragments shorter than     499 bp
  70% of sequence in fragments shorter than     554 bp
  75% of sequence in fragments shorter than     616 bp
  80% of sequence in fragments shorter than     693 bp
  85% of sequence in fragments shorter than     795 bp
  90% of sequence in fragments shorter than     943 bp
  95% of sequence in fragments shorter than    1206 bp

# AluI.AvaI (AGCT+CYCGRG) double digest, 14032 thousand fragments (13027778+1005714 sites), 212 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     180 bp
  25% of sequence in fragments shorter than     211 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     271 bp
  40% of sequence in fragments shorter than     304 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     371 bp
  55% of sequence in fragments shorter than     410 bp
  60% of sequence in fragments shorter than     453 bp
  65% of sequence in fragments shorter than     500 bp
  70% of sequence in fragments shorter than     554 bp
  75% of sequence in fragments shorter than     619 bp
  80% of sequence in fragments shorter than     696 bp
  85% of sequence in fragments shorter than     793 bp
  90% of sequence in fragments shorter than     935 bp
  95% of sequence in fragments shorter than    1191 bp

# CviQI.HinfI (GTAC+GANTC) double digest, 14030 thousand fragments (5115078+8916054 sites), 212 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     173 bp
  25% of sequence in fragments shorter than     201 bp
  30% of sequence in fragments shorter than     231 bp
  35% of sequence in fragments shorter than     261 bp
  40% of sequence in fragments shorter than     291 bp
  45% of sequence in fragments shorter than     322 bp
  50% of sequence in fragments shorter than     350 bp
  55% of sequence in fragments shorter than     385 bp
  60% of sequence in fragments shorter than     424 bp
  65% of sequence in fragments shorter than     467 bp
  70% of sequence in fragments shorter than     515 bp
  75% of sequence in fragments shorter than     567 bp
  80% of sequence in fragments shorter than     635 bp
  85% of sequence in fragments shorter than     715 bp
  90% of sequence in fragments shorter than     829 bp
  95% of sequence in fragments shorter than    1018 bp

# AluI.BspHI (AGCT+TCATGA) double digest, 14005 thousand fragments (13027778+978290 sites), 212 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     206 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     263 bp
  40% of sequence in fragments shorter than     294 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     359 bp
  55% of sequence in fragments shorter than     396 bp
  60% of sequence in fragments shorter than     437 bp
  65% of sequence in fragments shorter than     481 bp
  70% of sequence in fragments shorter than     532 bp
  75% of sequence in fragments shorter than     592 bp
  80% of sequence in fragments shorter than     666 bp
  85% of sequence in fragments shorter than     754 bp
  90% of sequence in fragments shorter than     887 bp
  95% of sequence in fragments shorter than    1126 bp

# AclI.CviAII (AACGTT+CATG) double digest, 13987 thousand fragments (172706+13815697 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     142 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     207 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     270 bp
  40% of sequence in fragments shorter than     301 bp
  45% of sequence in fragments shorter than     334 bp
  50% of sequence in fragments shorter than     370 bp
  55% of sequence in fragments shorter than     410 bp
  60% of sequence in fragments shorter than     453 bp
  65% of sequence in fragments shorter than     502 bp
  70% of sequence in fragments shorter than     557 bp
  75% of sequence in fragments shorter than     620 bp
  80% of sequence in fragments shorter than     697 bp
  85% of sequence in fragments shorter than     799 bp
  90% of sequence in fragments shorter than     945 bp
  95% of sequence in fragments shorter than    1198 bp

# CviAII.PacI (CATG+TTAATTAA) double digest, 13974 thousand fragments (13815697+159455 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     207 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     270 bp
  40% of sequence in fragments shorter than     301 bp
  45% of sequence in fragments shorter than     334 bp
  50% of sequence in fragments shorter than     370 bp
  55% of sequence in fragments shorter than     410 bp
  60% of sequence in fragments shorter than     454 bp
  65% of sequence in fragments shorter than     503 bp
  70% of sequence in fragments shorter than     558 bp
  75% of sequence in fragments shorter than     621 bp
  80% of sequence in fragments shorter than     698 bp
  85% of sequence in fragments shorter than     801 bp
  90% of sequence in fragments shorter than     948 bp
  95% of sequence in fragments shorter than    1215 bp

# CviAII.Hpy99I (CATG+CGWCG) double digest, 13973 thousand fragments (13815697+157917 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     208 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     270 bp
  40% of sequence in fragments shorter than     301 bp
  45% of sequence in fragments shorter than     334 bp
  50% of sequence in fragments shorter than     371 bp
  55% of sequence in fragments shorter than     410 bp
  60% of sequence in fragments shorter than     454 bp
  65% of sequence in fragments shorter than     502 bp
  70% of sequence in fragments shorter than     558 bp
  75% of sequence in fragments shorter than     620 bp
  80% of sequence in fragments shorter than     696 bp
  85% of sequence in fragments shorter than     797 bp
  90% of sequence in fragments shorter than     943 bp
  95% of sequence in fragments shorter than    1197 bp

# FokI.TspRI (GGATG+CASTG) double digest, 13965 thousand fragments (5201116+8765240 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     114 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     178 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     238 bp
  35% of sequence in fragments shorter than     270 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     339 bp
  50% of sequence in fragments shorter than     376 bp
  55% of sequence in fragments shorter than     417 bp
  60% of sequence in fragments shorter than     463 bp
  65% of sequence in fragments shorter than     514 bp
  70% of sequence in fragments shorter than     573 bp
  75% of sequence in fragments shorter than     641 bp
  80% of sequence in fragments shorter than     723 bp
  85% of sequence in fragments shorter than     825 bp
  90% of sequence in fragments shorter than     973 bp
  95% of sequence in fragments shorter than    1235 bp

# AluI.AvaIII (AGCT+ATGCAT) double digest, 13960 thousand fragments (13027778+933357 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     206 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     264 bp
  40% of sequence in fragments shorter than     295 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     358 bp
  55% of sequence in fragments shorter than     395 bp
  60% of sequence in fragments shorter than     436 bp
  65% of sequence in fragments shorter than     479 bp
  70% of sequence in fragments shorter than     530 bp
  75% of sequence in fragments shorter than     589 bp
  80% of sequence in fragments shorter than     661 bp
  85% of sequence in fragments shorter than     749 bp
  90% of sequence in fragments shorter than     881 bp
  95% of sequence in fragments shorter than    1117 bp

# AluI.StuI (AGCT+AGGCCT) double digest, 13953 thousand fragments (13027778+925729 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     206 bp
  30% of sequence in fragments shorter than     235 bp
  35% of sequence in fragments shorter than     266 bp
  40% of sequence in fragments shorter than     297 bp
  45% of sequence in fragments shorter than     331 bp
  50% of sequence in fragments shorter than     362 bp
  55% of sequence in fragments shorter than     400 bp
  60% of sequence in fragments shorter than     441 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     538 bp
  75% of sequence in fragments shorter than     598 bp
  80% of sequence in fragments shorter than     673 bp
  85% of sequence in fragments shorter than     761 bp
  90% of sequence in fragments shorter than     895 bp
  95% of sequence in fragments shorter than    1124 bp

# CviAII.SnaBI (CATG+TACGTA) double digest, 13947 thousand fragments (13815697+132299 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     208 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     271 bp
  40% of sequence in fragments shorter than     302 bp
  45% of sequence in fragments shorter than     335 bp
  50% of sequence in fragments shorter than     372 bp
  55% of sequence in fragments shorter than     411 bp
  60% of sequence in fragments shorter than     455 bp
  65% of sequence in fragments shorter than     504 bp
  70% of sequence in fragments shorter than     559 bp
  75% of sequence in fragments shorter than     622 bp
  80% of sequence in fragments shorter than     699 bp
  85% of sequence in fragments shorter than     802 bp
  90% of sequence in fragments shorter than     947 bp
  95% of sequence in fragments shorter than    1209 bp

# CviAII.NaeI (CATG+GCCGGC) double digest, 13940 thousand fragments (13815697+125692 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     208 bp
  30% of sequence in fragments shorter than     240 bp
  35% of sequence in fragments shorter than     271 bp
  40% of sequence in fragments shorter than     302 bp
  45% of sequence in fragments shorter than     335 bp
  50% of sequence in fragments shorter than     372 bp
  55% of sequence in fragments shorter than     411 bp
  60% of sequence in fragments shorter than     455 bp
  65% of sequence in fragments shorter than     504 bp
  70% of sequence in fragments shorter than     559 bp
  75% of sequence in fragments shorter than     622 bp
  80% of sequence in fragments shorter than     698 bp
  85% of sequence in fragments shorter than     801 bp
  90% of sequence in fragments shorter than     946 bp
  95% of sequence in fragments shorter than    1204 bp

# CviAII.XhoI (CATG+CTCGAG) double digest, 13936 thousand fragments (13815697+121308 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     208 bp
  30% of sequence in fragments shorter than     240 bp
  35% of sequence in fragments shorter than     271 bp
  40% of sequence in fragments shorter than     302 bp
  45% of sequence in fragments shorter than     335 bp
  50% of sequence in fragments shorter than     371 bp
  55% of sequence in fragments shorter than     411 bp
  60% of sequence in fragments shorter than     455 bp
  65% of sequence in fragments shorter than     504 bp
  70% of sequence in fragments shorter than     559 bp
  75% of sequence in fragments shorter than     622 bp
  80% of sequence in fragments shorter than     699 bp
  85% of sequence in fragments shorter than     802 bp
  90% of sequence in fragments shorter than     948 bp
  95% of sequence in fragments shorter than    1208 bp

# AluI.NdeI (AGCT+CATATG) double digest, 13930 thousand fragments (13027778+903360 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     146 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     207 bp
  30% of sequence in fragments shorter than     234 bp
  35% of sequence in fragments shorter than     264 bp
  40% of sequence in fragments shorter than     294 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     358 bp
  55% of sequence in fragments shorter than     395 bp
  60% of sequence in fragments shorter than     435 bp
  65% of sequence in fragments shorter than     479 bp
  70% of sequence in fragments shorter than     529 bp
  75% of sequence in fragments shorter than     588 bp
  80% of sequence in fragments shorter than     659 bp
  85% of sequence in fragments shorter than     748 bp
  90% of sequence in fragments shorter than     876 bp
  95% of sequence in fragments shorter than    1099 bp

# Hpy188I.TfiI (TCNGA+GAWTC) double digest, 13926 thousand fragments (8942153+4984362 sites), 213 bp average fragment length
   5% of sequence in fragments shorter than      76 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     178 bp
  25% of sequence in fragments shorter than     212 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     277 bp
  40% of sequence in fragments shorter than     310 bp
  45% of sequence in fragments shorter than     344 bp
  50% of sequence in fragments shorter than     382 bp
  55% of sequence in fragments shorter than     424 bp
  60% of sequence in fragments shorter than     469 bp
  65% of sequence in fragments shorter than     519 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     639 bp
  80% of sequence in fragments shorter than     714 bp
  85% of sequence in fragments shorter than     810 bp
  90% of sequence in fragments shorter than     944 bp
  95% of sequence in fragments shorter than    1170 bp

# BsmAI.BspNCI (GTCTC+CCAGA) double digest, 13913 thousand fragments (6631585+7282436 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     174 bp
  25% of sequence in fragments shorter than     202 bp
  30% of sequence in fragments shorter than     236 bp
  35% of sequence in fragments shorter than     271 bp
  40% of sequence in fragments shorter than     307 bp
  45% of sequence in fragments shorter than     345 bp
  50% of sequence in fragments shorter than     384 bp
  55% of sequence in fragments shorter than     427 bp
  60% of sequence in fragments shorter than     473 bp
  65% of sequence in fragments shorter than     528 bp
  70% of sequence in fragments shorter than     590 bp
  75% of sequence in fragments shorter than     664 bp
  80% of sequence in fragments shorter than     751 bp
  85% of sequence in fragments shorter than     866 bp
  90% of sequence in fragments shorter than    1022 bp
  95% of sequence in fragments shorter than    1301 bp

# ClaI.CviAII (ATCGAT+CATG) double digest, 13901 thousand fragments (86477+13815697 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     240 bp
  35% of sequence in fragments shorter than     272 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     336 bp
  50% of sequence in fragments shorter than     373 bp
  55% of sequence in fragments shorter than     413 bp
  60% of sequence in fragments shorter than     457 bp
  65% of sequence in fragments shorter than     506 bp
  70% of sequence in fragments shorter than     561 bp
  75% of sequence in fragments shorter than     625 bp
  80% of sequence in fragments shorter than     702 bp
  85% of sequence in fragments shorter than     806 bp
  90% of sequence in fragments shorter than     949 bp
  95% of sequence in fragments shorter than    1220 bp

# AluI.HindIII (AGCT+AAGCTT) double digest, 13887 thousand fragments (13027778+860365 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     318 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     429 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     577 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     820 bp
  90% of sequence in fragments shorter than     966 bp
  95% of sequence in fragments shorter than    1226 bp

# AatII.CviAII (GACGTC+CATG) double digest, 13886 thousand fragments (71089+13815697 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     272 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     373 bp
  55% of sequence in fragments shorter than     413 bp
  60% of sequence in fragments shorter than     457 bp
  65% of sequence in fragments shorter than     506 bp
  70% of sequence in fragments shorter than     562 bp
  75% of sequence in fragments shorter than     625 bp
  80% of sequence in fragments shorter than     703 bp
  85% of sequence in fragments shorter than     807 bp
  90% of sequence in fragments shorter than     950 bp
  95% of sequence in fragments shorter than    1219 bp

# CviAII.SacII (CATG+CCGCGG) double digest, 13882 thousand fragments (13815697+66901 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     272 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     373 bp
  55% of sequence in fragments shorter than     413 bp
  60% of sequence in fragments shorter than     457 bp
  65% of sequence in fragments shorter than     506 bp
  70% of sequence in fragments shorter than     561 bp
  75% of sequence in fragments shorter than     624 bp
  80% of sequence in fragments shorter than     701 bp
  85% of sequence in fragments shorter than     803 bp
  90% of sequence in fragments shorter than     948 bp
  95% of sequence in fragments shorter than    1206 bp

# CviQI.TspRI (GTAC+CASTG) double digest, 13879 thousand fragments (5115078+8765240 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      76 bp
  10% of sequence in fragments shorter than     116 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     214 bp
  30% of sequence in fragments shorter than     240 bp
  35% of sequence in fragments shorter than     271 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     335 bp
  50% of sequence in fragments shorter than     370 bp
  55% of sequence in fragments shorter than     409 bp
  60% of sequence in fragments shorter than     451 bp
  65% of sequence in fragments shorter than     498 bp
  70% of sequence in fragments shorter than     548 bp
  75% of sequence in fragments shorter than     606 bp
  80% of sequence in fragments shorter than     676 bp
  85% of sequence in fragments shorter than     766 bp
  90% of sequence in fragments shorter than     899 bp
  95% of sequence in fragments shorter than    1117 bp

# AluI.XbaI (AGCT+TCTAGA) double digest, 13878 thousand fragments (13027778+850998 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     178 bp
  25% of sequence in fragments shorter than     208 bp
  30% of sequence in fragments shorter than     236 bp
  35% of sequence in fragments shorter than     266 bp
  40% of sequence in fragments shorter than     297 bp
  45% of sequence in fragments shorter than     329 bp
  50% of sequence in fragments shorter than     362 bp
  55% of sequence in fragments shorter than     400 bp
  60% of sequence in fragments shorter than     440 bp
  65% of sequence in fragments shorter than     484 bp
  70% of sequence in fragments shorter than     535 bp
  75% of sequence in fragments shorter than     595 bp
  80% of sequence in fragments shorter than     667 bp
  85% of sequence in fragments shorter than     758 bp
  90% of sequence in fragments shorter than     889 bp
  95% of sequence in fragments shorter than    1118 bp

# BstNI.Tsp45I (CCWGG+GTSAC) double digest, 13876 thousand fragments (9855646+4021761 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     103 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     169 bp
  25% of sequence in fragments shorter than     205 bp
  30% of sequence in fragments shorter than     245 bp
  35% of sequence in fragments shorter than     287 bp
  40% of sequence in fragments shorter than     331 bp
  45% of sequence in fragments shorter than     376 bp
  50% of sequence in fragments shorter than     428 bp
  55% of sequence in fragments shorter than     487 bp
  60% of sequence in fragments shorter than     551 bp
  65% of sequence in fragments shorter than     624 bp
  70% of sequence in fragments shorter than     707 bp
  75% of sequence in fragments shorter than     812 bp
  80% of sequence in fragments shorter than     940 bp
  85% of sequence in fragments shorter than    1111 bp
  90% of sequence in fragments shorter than    1366 bp
  95% of sequence in fragments shorter than    1858 bp

# AluI.EcoRI (AGCT+GAATTC) double digest, 13874 thousand fragments (13027778+847401 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     178 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     237 bp
  35% of sequence in fragments shorter than     267 bp
  40% of sequence in fragments shorter than     298 bp
  45% of sequence in fragments shorter than     330 bp
  50% of sequence in fragments shorter than     360 bp
  55% of sequence in fragments shorter than     397 bp
  60% of sequence in fragments shorter than     439 bp
  65% of sequence in fragments shorter than     482 bp
  70% of sequence in fragments shorter than     533 bp
  75% of sequence in fragments shorter than     593 bp
  80% of sequence in fragments shorter than     666 bp
  85% of sequence in fragments shorter than     752 bp
  90% of sequence in fragments shorter than     882 bp
  95% of sequence in fragments shorter than    1103 bp

# CviAII.PspXI (CATG+VCTCGAGB) double digest, 13874 thousand fragments (13815697+59193 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     272 bp
  40% of sequence in fragments shorter than     304 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     374 bp
  55% of sequence in fragments shorter than     414 bp
  60% of sequence in fragments shorter than     458 bp
  65% of sequence in fragments shorter than     507 bp
  70% of sequence in fragments shorter than     563 bp
  75% of sequence in fragments shorter than     626 bp
  80% of sequence in fragments shorter than     704 bp
  85% of sequence in fragments shorter than     808 bp
  90% of sequence in fragments shorter than     951 bp
  95% of sequence in fragments shorter than    1221 bp

# AgeI.CviAII (ACCGGT+CATG) double digest, 13868 thousand fragments (53386+13815697 sites), 214 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     272 bp
  40% of sequence in fragments shorter than     304 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     374 bp
  55% of sequence in fragments shorter than     414 bp
  60% of sequence in fragments shorter than     458 bp
  65% of sequence in fragments shorter than     507 bp
  70% of sequence in fragments shorter than     563 bp
  75% of sequence in fragments shorter than     626 bp
  80% of sequence in fragments shorter than     704 bp
  85% of sequence in fragments shorter than     809 bp
  90% of sequence in fragments shorter than     952 bp
  95% of sequence in fragments shorter than    1223 bp

# CviAII.PmeI (CATG+GTTTAAAC) double digest, 13857 thousand fragments (13815697+42094 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     304 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     374 bp
  55% of sequence in fragments shorter than     414 bp
  60% of sequence in fragments shorter than     458 bp
  65% of sequence in fragments shorter than     508 bp
  70% of sequence in fragments shorter than     563 bp
  75% of sequence in fragments shorter than     627 bp
  80% of sequence in fragments shorter than     705 bp
  85% of sequence in fragments shorter than     809 bp
  90% of sequence in fragments shorter than     953 bp
  95% of sequence in fragments shorter than    1226 bp

# BbvI.HaeIII (GCAGC+GGCC) double digest, 13851 thousand fragments (5290051+8562230 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      62 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     184 bp
  25% of sequence in fragments shorter than     227 bp
  30% of sequence in fragments shorter than     270 bp
  35% of sequence in fragments shorter than     316 bp
  40% of sequence in fragments shorter than     356 bp
  45% of sequence in fragments shorter than     407 bp
  50% of sequence in fragments shorter than     460 bp
  55% of sequence in fragments shorter than     520 bp
  60% of sequence in fragments shorter than     586 bp
  65% of sequence in fragments shorter than     662 bp
  70% of sequence in fragments shorter than     747 bp
  75% of sequence in fragments shorter than     847 bp
  80% of sequence in fragments shorter than     973 bp
  85% of sequence in fragments shorter than    1139 bp
  90% of sequence in fragments shorter than    1362 bp
  95% of sequence in fragments shorter than    1759 bp

# CviAII.SalI (CATG+GTCGAC) double digest, 13846 thousand fragments (13815697+31396 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     304 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     508 bp
  70% of sequence in fragments shorter than     564 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     810 bp
  90% of sequence in fragments shorter than     953 bp
  95% of sequence in fragments shorter than    1225 bp

# CviAII.MluI (CATG+ACGCGT) double digest, 13836 thousand fragments (13815697+21415 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     811 bp
  90% of sequence in fragments shorter than     955 bp
  95% of sequence in fragments shorter than    1227 bp

# BsmAI.BspKT6I (GTCTC+GATC) double digest, 13830 thousand fragments (6631585+7199434 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     245 bp
  35% of sequence in fragments shorter than     282 bp
  40% of sequence in fragments shorter than     320 bp
  45% of sequence in fragments shorter than     359 bp
  50% of sequence in fragments shorter than     400 bp
  55% of sequence in fragments shorter than     445 bp
  60% of sequence in fragments shorter than     492 bp
  65% of sequence in fragments shorter than     546 bp
  70% of sequence in fragments shorter than     607 bp
  75% of sequence in fragments shorter than     678 bp
  80% of sequence in fragments shorter than     765 bp
  85% of sequence in fragments shorter than     877 bp
  90% of sequence in fragments shorter than    1036 bp
  95% of sequence in fragments shorter than    1323 bp

# CviAII.SgrAI (CATG+CRCCGGYG) double digest, 13830 thousand fragments (13815697+15270 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     810 bp
  90% of sequence in fragments shorter than     954 bp
  95% of sequence in fragments shorter than    1224 bp

# CviAII.NruI (CATG+TCGCGA) double digest, 13830 thousand fragments (13815697+14872 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     811 bp
  90% of sequence in fragments shorter than     955 bp
  95% of sequence in fragments shorter than    1226 bp

# CviAII.PvuI (CATG+CGATCG) double digest, 13829 thousand fragments (13815697+14186 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     811 bp
  90% of sequence in fragments shorter than     955 bp
  95% of sequence in fragments shorter than    1227 bp

# CviAII.FseI (CATG+GGCCGGCC) double digest, 13828 thousand fragments (13815697+13662 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     811 bp
  90% of sequence in fragments shorter than     955 bp
  95% of sequence in fragments shorter than    1226 bp

# CviAII.NotI (CATG+GCGGCCGC) double digest, 13824 thousand fragments (13815697+9795 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     810 bp
  90% of sequence in fragments shorter than     954 bp
  95% of sequence in fragments shorter than    1224 bp

# CviAII.RsrII (CATG+CGGWCCG) double digest, 13824 thousand fragments (13815697+9764 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     811 bp
  90% of sequence in fragments shorter than     955 bp
  95% of sequence in fragments shorter than    1226 bp

# AscI.CviAII (GGCGCGCC+CATG) double digest, 13819 thousand fragments (4751+13815697 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     416 bp
  60% of sequence in fragments shorter than     460 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     629 bp
  80% of sequence in fragments shorter than     707 bp
  85% of sequence in fragments shorter than     811 bp
  90% of sequence in fragments shorter than     955 bp
  95% of sequence in fragments shorter than    1227 bp

# AluI.BglII (AGCT+AGATCT) double digest, 13802 thousand fragments (13027778+774732 sites), 215 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     178 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     237 bp
  35% of sequence in fragments shorter than     267 bp
  40% of sequence in fragments shorter than     299 bp
  45% of sequence in fragments shorter than     331 bp
  50% of sequence in fragments shorter than     364 bp
  55% of sequence in fragments shorter than     401 bp
  60% of sequence in fragments shorter than     443 bp
  65% of sequence in fragments shorter than     487 bp
  70% of sequence in fragments shorter than     540 bp
  75% of sequence in fragments shorter than     600 bp
  80% of sequence in fragments shorter than     676 bp
  85% of sequence in fragments shorter than     766 bp
  90% of sequence in fragments shorter than     901 bp
  95% of sequence in fragments shorter than    1144 bp

# AluI.NcoI (AGCT+CCATGG) double digest, 13786 thousand fragments (13027778+759594 sites), 216 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     238 bp
  35% of sequence in fragments shorter than     269 bp
  40% of sequence in fragments shorter than     301 bp
  45% of sequence in fragments shorter than     334 bp
  50% of sequence in fragments shorter than     367 bp
  55% of sequence in fragments shorter than     405 bp
  60% of sequence in fragments shorter than     447 bp
  65% of sequence in fragments shorter than     493 bp
  70% of sequence in fragments shorter than     545 bp
  75% of sequence in fragments shorter than     607 bp
  80% of sequence in fragments shorter than     679 bp
  85% of sequence in fragments shorter than     772 bp
  90% of sequence in fragments shorter than     907 bp
  95% of sequence in fragments shorter than    1150 bp

# BstNI.MlyI (CCWGG+GAGTC) double digest, 13786 thousand fragments (9855646+3931692 sites), 216 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     135 bp
  20% of sequence in fragments shorter than     170 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     250 bp
  35% of sequence in fragments shorter than     294 bp
  40% of sequence in fragments shorter than     339 bp
  45% of sequence in fragments shorter than     389 bp
  50% of sequence in fragments shorter than     445 bp
  55% of sequence in fragments shorter than     507 bp
  60% of sequence in fragments shorter than     577 bp
  65% of sequence in fragments shorter than     655 bp
  70% of sequence in fragments shorter than     747 bp
  75% of sequence in fragments shorter than     859 bp
  80% of sequence in fragments shorter than     998 bp
  85% of sequence in fragments shorter than    1188 bp
  90% of sequence in fragments shorter than    1478 bp
  95% of sequence in fragments shorter than    2057 bp

# AluI.BclI (AGCT+TGATCA) double digest, 13766 thousand fragments (13027778+738785 sites), 216 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     269 bp
  40% of sequence in fragments shorter than     300 bp
  45% of sequence in fragments shorter than     333 bp
  50% of sequence in fragments shorter than     366 bp
  55% of sequence in fragments shorter than     404 bp
  60% of sequence in fragments shorter than     445 bp
  65% of sequence in fragments shorter than     490 bp
  70% of sequence in fragments shorter than     542 bp
  75% of sequence in fragments shorter than     603 bp
  80% of sequence in fragments shorter than     679 bp
  85% of sequence in fragments shorter than     770 bp
  90% of sequence in fragments shorter than     907 bp
  95% of sequence in fragments shorter than    1150 bp

# FokI.HaeIII (GGATG+GGCC) double digest, 13762 thousand fragments (5201116+8562230 sites), 216 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     146 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     224 bp
  30% of sequence in fragments shorter than     262 bp
  35% of sequence in fragments shorter than     302 bp
  40% of sequence in fragments shorter than     343 bp
  45% of sequence in fragments shorter than     381 bp
  50% of sequence in fragments shorter than     429 bp
  55% of sequence in fragments shorter than     480 bp
  60% of sequence in fragments shorter than     536 bp
  65% of sequence in fragments shorter than     601 bp
  70% of sequence in fragments shorter than     676 bp
  75% of sequence in fragments shorter than     760 bp
  80% of sequence in fragments shorter than     861 bp
  85% of sequence in fragments shorter than     996 bp
  90% of sequence in fragments shorter than    1187 bp
  95% of sequence in fragments shorter than    1499 bp

# AluI.FnuDII (AGCT+CGCG) double digest, 13761 thousand fragments (13027778+733940 sites), 216 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     211 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     306 bp
  45% of sequence in fragments shorter than     339 bp
  50% of sequence in fragments shorter than     374 bp
  55% of sequence in fragments shorter than     415 bp
  60% of sequence in fragments shorter than     458 bp
  65% of sequence in fragments shorter than     506 bp
  70% of sequence in fragments shorter than     561 bp
  75% of sequence in fragments shorter than     626 bp
  80% of sequence in fragments shorter than     702 bp
  85% of sequence in fragments shorter than     801 bp
  90% of sequence in fragments shorter than     945 bp
  95% of sequence in fragments shorter than    1202 bp

# TfiI.TspRI (GAWTC+CASTG) double digest, 13749 thousand fragments (4984362+8765240 sites), 216 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     119 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     184 bp
  25% of sequence in fragments shorter than     214 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     274 bp
  40% of sequence in fragments shorter than     307 bp
  45% of sequence in fragments shorter than     341 bp
  50% of sequence in fragments shorter than     372 bp
  55% of sequence in fragments shorter than     411 bp
  60% of sequence in fragments shorter than     454 bp
  65% of sequence in fragments shorter than     502 bp
  70% of sequence in fragments shorter than     555 bp
  75% of sequence in fragments shorter than     618 bp
  80% of sequence in fragments shorter than     690 bp
  85% of sequence in fragments shorter than     783 bp
  90% of sequence in fragments shorter than     913 bp
  95% of sequence in fragments shorter than    1136 bp

# AluI.BsaAI (AGCT+YACGTR) double digest, 13741 thousand fragments (13027778+713801 sites), 216 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     180 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     269 bp
  40% of sequence in fragments shorter than     301 bp
  45% of sequence in fragments shorter than     333 bp
  50% of sequence in fragments shorter than     366 bp
  55% of sequence in fragments shorter than     404 bp
  60% of sequence in fragments shorter than     446 bp
  65% of sequence in fragments shorter than     492 bp
  70% of sequence in fragments shorter than     542 bp
  75% of sequence in fragments shorter than     602 bp
  80% of sequence in fragments shorter than     677 bp
  85% of sequence in fragments shorter than     769 bp
  90% of sequence in fragments shorter than     902 bp
  95% of sequence in fragments shorter than    1133 bp

# AluI.BceAI (AGCT+ACGGC) double digest, 13732 thousand fragments (13027778+705178 sites), 217 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     270 bp
  40% of sequence in fragments shorter than     302 bp
  45% of sequence in fragments shorter than     336 bp
  50% of sequence in fragments shorter than     369 bp
  55% of sequence in fragments shorter than     408 bp
  60% of sequence in fragments shorter than     451 bp
  65% of sequence in fragments shorter than     497 bp
  70% of sequence in fragments shorter than     551 bp
  75% of sequence in fragments shorter than     614 bp
  80% of sequence in fragments shorter than     690 bp
  85% of sequence in fragments shorter than     786 bp
  90% of sequence in fragments shorter than     926 bp
  95% of sequence in fragments shorter than    1179 bp

# AluI.BciVI (AGCT+GTATCC) double digest, 13723 thousand fragments (13027778+696093 sites), 217 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     239 bp
  35% of sequence in fragments shorter than     269 bp
  40% of sequence in fragments shorter than     300 bp
  45% of sequence in fragments shorter than     332 bp
  50% of sequence in fragments shorter than     366 bp
  55% of sequence in fragments shorter than     404 bp
  60% of sequence in fragments shorter than     445 bp
  65% of sequence in fragments shorter than     490 bp
  70% of sequence in fragments shorter than     542 bp
  75% of sequence in fragments shorter than     603 bp
  80% of sequence in fragments shorter than     679 bp
  85% of sequence in fragments shorter than     770 bp
  90% of sequence in fragments shorter than     907 bp
  95% of sequence in fragments shorter than    1149 bp

# BsmAI.Hin4II (GTCTC+CCTTC) double digest, 13690 thousand fragments (6631585+7059915 sites), 217 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     245 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     316 bp
  45% of sequence in fragments shorter than     353 bp
  50% of sequence in fragments shorter than     394 bp
  55% of sequence in fragments shorter than     439 bp
  60% of sequence in fragments shorter than     489 bp
  65% of sequence in fragments shorter than     543 bp
  70% of sequence in fragments shorter than     607 bp
  75% of sequence in fragments shorter than     682 bp
  80% of sequence in fragments shorter than     774 bp
  85% of sequence in fragments shorter than     892 bp
  90% of sequence in fragments shorter than    1052 bp
  95% of sequence in fragments shorter than    1345 bp

# CviQI.HaeIII (GTAC+GGCC) double digest, 13676 thousand fragments (5115078+8562230 sites), 217 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     182 bp
  25% of sequence in fragments shorter than     218 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     287 bp
  40% of sequence in fragments shorter than     324 bp
  45% of sequence in fragments shorter than     356 bp
  50% of sequence in fragments shorter than     397 bp
  55% of sequence in fragments shorter than     440 bp
  60% of sequence in fragments shorter than     489 bp
  65% of sequence in fragments shorter than     539 bp
  70% of sequence in fragments shorter than     597 bp
  75% of sequence in fragments shorter than     662 bp
  80% of sequence in fragments shorter than     740 bp
  85% of sequence in fragments shorter than     847 bp
  90% of sequence in fragments shorter than     994 bp
  95% of sequence in fragments shorter than    1240 bp

# AflII.AluI (CTTAAG+AGCT) double digest, 13670 thousand fragments (643284+13027778 sites), 217 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     181 bp
  25% of sequence in fragments shorter than     212 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     272 bp
  40% of sequence in fragments shorter than     304 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     370 bp
  55% of sequence in fragments shorter than     409 bp
  60% of sequence in fragments shorter than     451 bp
  65% of sequence in fragments shorter than     496 bp
  70% of sequence in fragments shorter than     549 bp
  75% of sequence in fragments shorter than     611 bp
  80% of sequence in fragments shorter than     687 bp
  85% of sequence in fragments shorter than     780 bp
  90% of sequence in fragments shorter than     918 bp
  95% of sequence in fragments shorter than    1166 bp

# ApoI.BspNCI (RAATTY+CCAGA) double digest, 13664 thousand fragments (6382433+7282436 sites), 218 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     114 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     210 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     308 bp
  45% of sequence in fragments shorter than     343 bp
  50% of sequence in fragments shorter than     379 bp
  55% of sequence in fragments shorter than     419 bp
  60% of sequence in fragments shorter than     463 bp
  65% of sequence in fragments shorter than     512 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     704 bp
  85% of sequence in fragments shorter than     804 bp
  90% of sequence in fragments shorter than     938 bp
  95% of sequence in fragments shorter than    1151 bp

# AluI.HaeII (AGCT+RGCGCY) double digest, 13657 thousand fragments (13027778+630101 sites), 218 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     180 bp
  25% of sequence in fragments shorter than     212 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     306 bp
  45% of sequence in fragments shorter than     339 bp
  50% of sequence in fragments shorter than     373 bp
  55% of sequence in fragments shorter than     413 bp
  60% of sequence in fragments shorter than     455 bp
  65% of sequence in fragments shorter than     502 bp
  70% of sequence in fragments shorter than     555 bp
  75% of sequence in fragments shorter than     619 bp
  80% of sequence in fragments shorter than     694 bp
  85% of sequence in fragments shorter than     789 bp
  90% of sequence in fragments shorter than     929 bp
  95% of sequence in fragments shorter than    1166 bp

# AluI.SacI (AGCT+GAGCTC) double digest, 13630 thousand fragments (13027778+603323 sites), 218 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     318 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     388 bp
  55% of sequence in fragments shorter than     429 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     577 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     820 bp
  90% of sequence in fragments shorter than     966 bp
  95% of sequence in fragments shorter than    1226 bp

# AluI.AvrII (AGCT+CCTAGG) double digest, 13622 thousand fragments (13027778+594956 sites), 218 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     180 bp
  25% of sequence in fragments shorter than     211 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     272 bp
  40% of sequence in fragments shorter than     304 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     371 bp
  55% of sequence in fragments shorter than     411 bp
  60% of sequence in fragments shorter than     453 bp
  65% of sequence in fragments shorter than     500 bp
  70% of sequence in fragments shorter than     554 bp
  75% of sequence in fragments shorter than     617 bp
  80% of sequence in fragments shorter than     693 bp
  85% of sequence in fragments shorter than     788 bp
  90% of sequence in fragments shorter than     929 bp
  95% of sequence in fragments shorter than    1181 bp

# AluI.HgaI (AGCT+GACGC) double digest, 13599 thousand fragments (13027778+571889 sites), 219 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     149 bp
  20% of sequence in fragments shorter than     181 bp
  25% of sequence in fragments shorter than     213 bp
  30% of sequence in fragments shorter than     242 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     305 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     372 bp
  55% of sequence in fragments shorter than     412 bp
  60% of sequence in fragments shorter than     455 bp
  65% of sequence in fragments shorter than     502 bp
  70% of sequence in fragments shorter than     556 bp
  75% of sequence in fragments shorter than     619 bp
  80% of sequence in fragments shorter than     696 bp
  85% of sequence in fragments shorter than     793 bp
  90% of sequence in fragments shorter than     935 bp
  95% of sequence in fragments shorter than    1188 bp

# AluI.EciI (AGCT+GGCGGA) double digest, 13598 thousand fragments (13027778+571273 sites), 219 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     181 bp
  25% of sequence in fragments shorter than     213 bp
  30% of sequence in fragments shorter than     243 bp
  35% of sequence in fragments shorter than     275 bp
  40% of sequence in fragments shorter than     308 bp
  45% of sequence in fragments shorter than     341 bp
  50% of sequence in fragments shorter than     377 bp
  55% of sequence in fragments shorter than     417 bp
  60% of sequence in fragments shorter than     460 bp
  65% of sequence in fragments shorter than     508 bp
  70% of sequence in fragments shorter than     563 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     704 bp
  85% of sequence in fragments shorter than     803 bp
  90% of sequence in fragments shorter than     947 bp
  95% of sequence in fragments shorter than    1204 bp

# AluI.MfeI (AGCT+CAATTG) double digest, 13591 thousand fragments (13027778+564303 sites), 219 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     149 bp
  20% of sequence in fragments shorter than     181 bp
  25% of sequence in fragments shorter than     212 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     271 bp
  40% of sequence in fragments shorter than     303 bp
  45% of sequence in fragments shorter than     336 bp
  50% of sequence in fragments shorter than     370 bp
  55% of sequence in fragments shorter than     408 bp
  60% of sequence in fragments shorter than     450 bp
  65% of sequence in fragments shorter than     495 bp
  70% of sequence in fragments shorter than     548 bp
  75% of sequence in fragments shorter than     609 bp
  80% of sequence in fragments shorter than     685 bp
  85% of sequence in fragments shorter than     776 bp
  90% of sequence in fragments shorter than     911 bp
  95% of sequence in fragments shorter than    1148 bp

# ApoI.BspKT6I (RAATTY+GATC) double digest, 13581 thousand fragments (6382433+7199434 sites), 219 bp average fragment length
   5% of sequence in fragments shorter than      77 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     199 bp
  30% of sequence in fragments shorter than     231 bp
  35% of sequence in fragments shorter than     261 bp
  40% of sequence in fragments shorter than     293 bp
  45% of sequence in fragments shorter than     326 bp
  50% of sequence in fragments shorter than     361 bp
  55% of sequence in fragments shorter than     400 bp
  60% of sequence in fragments shorter than     442 bp
  65% of sequence in fragments shorter than     490 bp
  70% of sequence in fragments shorter than     544 bp
  75% of sequence in fragments shorter than     607 bp
  80% of sequence in fragments shorter than     684 bp
  85% of sequence in fragments shorter than     781 bp
  90% of sequence in fragments shorter than     917 bp
  95% of sequence in fragments shorter than    1167 bp

# AluI.SphI (AGCT+GCATGC) double digest, 13578 thousand fragments (13027778+550952 sites), 219 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     114 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     214 bp
  30% of sequence in fragments shorter than     243 bp
  35% of sequence in fragments shorter than     274 bp
  40% of sequence in fragments shorter than     306 bp
  45% of sequence in fragments shorter than     339 bp
  50% of sequence in fragments shorter than     373 bp
  55% of sequence in fragments shorter than     413 bp
  60% of sequence in fragments shorter than     455 bp
  65% of sequence in fragments shorter than     502 bp
  70% of sequence in fragments shorter than     555 bp
  75% of sequence in fragments shorter than     619 bp
  80% of sequence in fragments shorter than     695 bp
  85% of sequence in fragments shorter than     790 bp
  90% of sequence in fragments shorter than     930 bp
  95% of sequence in fragments shorter than    1182 bp

# AluI.ScaI (AGCT+AGTACT) double digest, 13571 thousand fragments (13027778+543793 sites), 219 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     149 bp
  20% of sequence in fragments shorter than     181 bp
  25% of sequence in fragments shorter than     212 bp
  30% of sequence in fragments shorter than     241 bp
  35% of sequence in fragments shorter than     272 bp
  40% of sequence in fragments shorter than     304 bp
  45% of sequence in fragments shorter than     337 bp
  50% of sequence in fragments shorter than     370 bp
  55% of sequence in fragments shorter than     409 bp
  60% of sequence in fragments shorter than     450 bp
  65% of sequence in fragments shorter than     496 bp
  70% of sequence in fragments shorter than     549 bp
  75% of sequence in fragments shorter than     611 bp
  80% of sequence in fragments shorter than     686 bp
  85% of sequence in fragments shorter than     779 bp
  90% of sequence in fragments shorter than     917 bp
  95% of sequence in fragments shorter than    1163 bp

# HhaI.ScrFI (GCGC+CCNGG) double digest, 13561 thousand fragments (1756502+11805099 sites), 219 bp average fragment length
   5% of sequence in fragments shorter than      59 bp
  10% of sequence in fragments shorter than      97 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     240 bp
  30% of sequence in fragments shorter than     296 bp
  35% of sequence in fragments shorter than     351 bp
  40% of sequence in fragments shorter than     415 bp
  45% of sequence in fragments shorter than     486 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     648 bp
  60% of sequence in fragments shorter than     733 bp
  65% of sequence in fragments shorter than     844 bp
  70% of sequence in fragments shorter than     972 bp
  75% of sequence in fragments shorter than    1122 bp
  80% of sequence in fragments shorter than    1312 bp
  85% of sequence in fragments shorter than    1560 bp
  90% of sequence in fragments shorter than    1897 bp
  95% of sequence in fragments shorter than    2479 bp

# HaeIII.TfiI (GGCC+GAWTC) double digest, 13546 thousand fragments (8562230+4984362 sites), 219 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     211 bp
  30% of sequence in fragments shorter than     247 bp
  35% of sequence in fragments shorter than     285 bp
  40% of sequence in fragments shorter than     323 bp
  45% of sequence in fragments shorter than     363 bp
  50% of sequence in fragments shorter than     406 bp
  55% of sequence in fragments shorter than     454 bp
  60% of sequence in fragments shorter than     506 bp
  65% of sequence in fragments shorter than     564 bp
  70% of sequence in fragments shorter than     631 bp
  75% of sequence in fragments shorter than     709 bp
  80% of sequence in fragments shorter than     802 bp
  85% of sequence in fragments shorter than     922 bp
  90% of sequence in fragments shorter than    1090 bp
  95% of sequence in fragments shorter than    1381 bp

# AluI.ApaLI (AGCT+GTGCAC) double digest, 13523 thousand fragments (13027778+496255 sites), 220 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     114 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     214 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     274 bp
  40% of sequence in fragments shorter than     307 bp
  45% of sequence in fragments shorter than     339 bp
  50% of sequence in fragments shorter than     374 bp
  55% of sequence in fragments shorter than     413 bp
  60% of sequence in fragments shorter than     456 bp
  65% of sequence in fragments shorter than     502 bp
  70% of sequence in fragments shorter than     556 bp
  75% of sequence in fragments shorter than     619 bp
  80% of sequence in fragments shorter than     695 bp
  85% of sequence in fragments shorter than     790 bp
  90% of sequence in fragments shorter than     929 bp
  95% of sequence in fragments shorter than    1170 bp

# BfiI.ScrFI (ACTGGG+CCNGG) double digest, 13511 thousand fragments (1706594+11805099 sites), 220 bp average fragment length
   5% of sequence in fragments shorter than      61 bp
  10% of sequence in fragments shorter than      96 bp
  15% of sequence in fragments shorter than     134 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     269 bp
  35% of sequence in fragments shorter than     321 bp
  40% of sequence in fragments shorter than     374 bp
  45% of sequence in fragments shorter than     434 bp
  50% of sequence in fragments shorter than     503 bp
  55% of sequence in fragments shorter than     578 bp
  60% of sequence in fragments shorter than     661 bp
  65% of sequence in fragments shorter than     754 bp
  70% of sequence in fragments shorter than     868 bp
  75% of sequence in fragments shorter than    1003 bp
  80% of sequence in fragments shorter than    1173 bp
  85% of sequence in fragments shorter than    1399 bp
  90% of sequence in fragments shorter than    1712 bp
  95% of sequence in fragments shorter than    2316 bp

# BscAI.BstNI (GCATC+CCWGG) double digest, 13508 thousand fragments (3652926+9855646 sites), 220 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     171 bp
  25% of sequence in fragments shorter than     209 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     291 bp
  40% of sequence in fragments shorter than     336 bp
  45% of sequence in fragments shorter than     384 bp
  50% of sequence in fragments shorter than     436 bp
  55% of sequence in fragments shorter than     493 bp
  60% of sequence in fragments shorter than     555 bp
  65% of sequence in fragments shorter than     624 bp
  70% of sequence in fragments shorter than     707 bp
  75% of sequence in fragments shorter than     805 bp
  80% of sequence in fragments shorter than     932 bp
  85% of sequence in fragments shorter than    1101 bp
  90% of sequence in fragments shorter than    1344 bp
  95% of sequence in fragments shorter than    1788 bp

# BspNCI.BthCI (CCAGA+GCNGC) double digest, 13491 thousand fragments (7282436+6209929 sites), 220 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     191 bp
  25% of sequence in fragments shorter than     230 bp
  30% of sequence in fragments shorter than     270 bp
  35% of sequence in fragments shorter than     311 bp
  40% of sequence in fragments shorter than     352 bp
  45% of sequence in fragments shorter than     398 bp
  50% of sequence in fragments shorter than     446 bp
  55% of sequence in fragments shorter than     499 bp
  60% of sequence in fragments shorter than     558 bp
  65% of sequence in fragments shorter than     620 bp
  70% of sequence in fragments shorter than     692 bp
  75% of sequence in fragments shorter than     776 bp
  80% of sequence in fragments shorter than     881 bp
  85% of sequence in fragments shorter than    1017 bp
  90% of sequence in fragments shorter than    1195 bp
  95% of sequence in fragments shorter than    1509 bp

# AluI.ApaI (AGCT+GGGCCC) double digest, 13487 thousand fragments (13027778+460339 sites), 220 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     114 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     214 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     275 bp
  40% of sequence in fragments shorter than     308 bp
  45% of sequence in fragments shorter than     341 bp
  50% of sequence in fragments shorter than     376 bp
  55% of sequence in fragments shorter than     416 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     506 bp
  70% of sequence in fragments shorter than     561 bp
  75% of sequence in fragments shorter than     626 bp
  80% of sequence in fragments shorter than     702 bp
  85% of sequence in fragments shorter than     801 bp
  90% of sequence in fragments shorter than     945 bp
  95% of sequence in fragments shorter than    1202 bp

# AluI.EcoRV (AGCT+GATATC) double digest, 13473 thousand fragments (13027778+446473 sites), 221 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     114 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     213 bp
  30% of sequence in fragments shorter than     243 bp
  35% of sequence in fragments shorter than     273 bp
  40% of sequence in fragments shorter than     306 bp
  45% of sequence in fragments shorter than     338 bp
  50% of sequence in fragments shorter than     372 bp
  55% of sequence in fragments shorter than     412 bp
  60% of sequence in fragments shorter than     454 bp
  65% of sequence in fragments shorter than     499 bp
  70% of sequence in fragments shorter than     553 bp
  75% of sequence in fragments shorter than     615 bp
  80% of sequence in fragments shorter than     691 bp
  85% of sequence in fragments shorter than     784 bp
  90% of sequence in fragments shorter than     922 bp
  95% of sequence in fragments shorter than    1168 bp

# BccI.BspNCI (CCATC+CCAGA) double digest, 13452 thousand fragments (6170927+7282436 sites), 221 bp average fragment length
   5% of sequence in fragments shorter than      76 bp
  10% of sequence in fragments shorter than     116 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     214 bp
  30% of sequence in fragments shorter than     248 bp
  35% of sequence in fragments shorter than     282 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     352 bp
  50% of sequence in fragments shorter than     391 bp
  55% of sequence in fragments shorter than     433 bp
  60% of sequence in fragments shorter than     478 bp
  65% of sequence in fragments shorter than     530 bp
  70% of sequence in fragments shorter than     591 bp
  75% of sequence in fragments shorter than     661 bp
  80% of sequence in fragments shorter than     744 bp
  85% of sequence in fragments shorter than     856 bp
  90% of sequence in fragments shorter than    1012 bp
  95% of sequence in fragments shorter than    1282 bp

# PsiI.ScrFI (TTATAA+CCNGG) double digest, 13452 thousand fragments (1647912+11805099 sites), 221 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     218 bp
  30% of sequence in fragments shorter than     262 bp
  35% of sequence in fragments shorter than     308 bp
  40% of sequence in fragments shorter than     355 bp
  45% of sequence in fragments shorter than     406 bp
  50% of sequence in fragments shorter than     462 bp
  55% of sequence in fragments shorter than     524 bp
  60% of sequence in fragments shorter than     592 bp
  65% of sequence in fragments shorter than     667 bp
  70% of sequence in fragments shorter than     754 bp
  75% of sequence in fragments shorter than     861 bp
  80% of sequence in fragments shorter than     992 bp
  85% of sequence in fragments shorter than    1169 bp
  90% of sequence in fragments shorter than    1433 bp
  95% of sequence in fragments shorter than    1961 bp

# ApoI.Hin4II (RAATTY+CCTTC) double digest, 13441 thousand fragments (6382433+7059915 sites), 221 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     185 bp
  25% of sequence in fragments shorter than     217 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     283 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     353 bp
  50% of sequence in fragments shorter than     393 bp
  55% of sequence in fragments shorter than     436 bp
  60% of sequence in fragments shorter than     483 bp
  65% of sequence in fragments shorter than     531 bp
  70% of sequence in fragments shorter than     589 bp
  75% of sequence in fragments shorter than     655 bp
  80% of sequence in fragments shorter than     734 bp
  85% of sequence in fragments shorter than     837 bp
  90% of sequence in fragments shorter than     977 bp
  95% of sequence in fragments shorter than    1221 bp

# AluI.SpeI (AGCT+ACTAGT) double digest, 13427 thousand fragments (13027778+400286 sites), 221 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     184 bp
  25% of sequence in fragments shorter than     215 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     275 bp
  40% of sequence in fragments shorter than     307 bp
  45% of sequence in fragments shorter than     339 bp
  50% of sequence in fragments shorter than     374 bp
  55% of sequence in fragments shorter than     411 bp
  60% of sequence in fragments shorter than     453 bp
  65% of sequence in fragments shorter than     499 bp
  70% of sequence in fragments shorter than     552 bp
  75% of sequence in fragments shorter than     614 bp
  80% of sequence in fragments shorter than     689 bp
  85% of sequence in fragments shorter than     782 bp
  90% of sequence in fragments shorter than     916 bp
  95% of sequence in fragments shorter than    1157 bp

# AluI.HpaI (AGCT+GTTAAC) double digest, 13416 thousand fragments (13027778+389418 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     184 bp
  25% of sequence in fragments shorter than     215 bp
  30% of sequence in fragments shorter than     244 bp
  35% of sequence in fragments shorter than     275 bp
  40% of sequence in fragments shorter than     308 bp
  45% of sequence in fragments shorter than     340 bp
  50% of sequence in fragments shorter than     375 bp
  55% of sequence in fragments shorter than     414 bp
  60% of sequence in fragments shorter than     457 bp
  65% of sequence in fragments shorter than     503 bp
  70% of sequence in fragments shorter than     557 bp
  75% of sequence in fragments shorter than     619 bp
  80% of sequence in fragments shorter than     695 bp
  85% of sequence in fragments shorter than     790 bp
  90% of sequence in fragments shorter than     930 bp
  95% of sequence in fragments shorter than    1181 bp

# BspKT6I.BthCI (GATC+GCNGC) double digest, 13408 thousand fragments (7199434+6209929 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     120 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     181 bp
  25% of sequence in fragments shorter than     216 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     288 bp
  40% of sequence in fragments shorter than     326 bp
  45% of sequence in fragments shorter than     364 bp
  50% of sequence in fragments shorter than     407 bp
  55% of sequence in fragments shorter than     453 bp
  60% of sequence in fragments shorter than     502 bp
  65% of sequence in fragments shorter than     558 bp
  70% of sequence in fragments shorter than     621 bp
  75% of sequence in fragments shorter than     695 bp
  80% of sequence in fragments shorter than     789 bp
  85% of sequence in fragments shorter than     909 bp
  90% of sequence in fragments shorter than    1068 bp
  95% of sequence in fragments shorter than    1357 bp

# EsaBC3I.ScrFI (TCGA+CCNGG) double digest, 13407 thousand fragments (1603346+11805099 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     136 bp
  20% of sequence in fragments shorter than     181 bp
  25% of sequence in fragments shorter than     228 bp
  30% of sequence in fragments shorter than     279 bp
  35% of sequence in fragments shorter than     332 bp
  40% of sequence in fragments shorter than     390 bp
  45% of sequence in fragments shorter than     452 bp
  50% of sequence in fragments shorter than     520 bp
  55% of sequence in fragments shorter than     596 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     775 bp
  70% of sequence in fragments shorter than     887 bp
  75% of sequence in fragments shorter than    1024 bp
  80% of sequence in fragments shorter than    1193 bp
  85% of sequence in fragments shorter than    1407 bp
  90% of sequence in fragments shorter than    1734 bp
  95% of sequence in fragments shorter than    2287 bp

# AciI.MboII (CCGC+GAAGA) double digest, 13405 thousand fragments (4206130+9199496 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      77 bp
  10% of sequence in fragments shorter than     121 bp
  15% of sequence in fragments shorter than     158 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     225 bp
  30% of sequence in fragments shorter than     262 bp
  35% of sequence in fragments shorter than     300 bp
  40% of sequence in fragments shorter than     337 bp
  45% of sequence in fragments shorter than     375 bp
  50% of sequence in fragments shorter than     418 bp
  55% of sequence in fragments shorter than     464 bp
  60% of sequence in fragments shorter than     514 bp
  65% of sequence in fragments shorter than     568 bp
  70% of sequence in fragments shorter than     631 bp
  75% of sequence in fragments shorter than     704 bp
  80% of sequence in fragments shorter than     791 bp
  85% of sequence in fragments shorter than     900 bp
  90% of sequence in fragments shorter than    1046 bp
  95% of sequence in fragments shorter than    1299 bp

# AluI.SapI (AGCT+GCTCTTC) double digest, 13404 thousand fragments (13027778+377161 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     116 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     186 bp
  25% of sequence in fragments shorter than     217 bp
  30% of sequence in fragments shorter than     247 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     311 bp
  45% of sequence in fragments shorter than     344 bp
  50% of sequence in fragments shorter than     379 bp
  55% of sequence in fragments shorter than     420 bp
  60% of sequence in fragments shorter than     462 bp
  65% of sequence in fragments shorter than     510 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     630 bp
  80% of sequence in fragments shorter than     706 bp
  85% of sequence in fragments shorter than     805 bp
  90% of sequence in fragments shorter than     949 bp
  95% of sequence in fragments shorter than    1206 bp

# AluI.SmaI (AGCT+CCCGGG) double digest, 13404 thousand fragments (13027778+376940 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     186 bp
  25% of sequence in fragments shorter than     217 bp
  30% of sequence in fragments shorter than     247 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     312 bp
  45% of sequence in fragments shorter than     345 bp
  50% of sequence in fragments shorter than     381 bp
  55% of sequence in fragments shorter than     422 bp
  60% of sequence in fragments shorter than     465 bp
  65% of sequence in fragments shorter than     513 bp
  70% of sequence in fragments shorter than     568 bp
  75% of sequence in fragments shorter than     633 bp
  80% of sequence in fragments shorter than     709 bp
  85% of sequence in fragments shorter than     809 bp
  90% of sequence in fragments shorter than     954 bp
  95% of sequence in fragments shorter than    1213 bp

# AluI.BamHI (AGCT+GGATCC) double digest, 13393 thousand fragments (13027778+365999 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     185 bp
  25% of sequence in fragments shorter than     215 bp
  30% of sequence in fragments shorter than     245 bp
  35% of sequence in fragments shorter than     277 bp
  40% of sequence in fragments shorter than     310 bp
  45% of sequence in fragments shorter than     342 bp
  50% of sequence in fragments shorter than     377 bp
  55% of sequence in fragments shorter than     418 bp
  60% of sequence in fragments shorter than     460 bp
  65% of sequence in fragments shorter than     508 bp
  70% of sequence in fragments shorter than     562 bp
  75% of sequence in fragments shorter than     626 bp
  80% of sequence in fragments shorter than     702 bp
  85% of sequence in fragments shorter than     800 bp
  90% of sequence in fragments shorter than     942 bp
  95% of sequence in fragments shorter than    1197 bp

# BstNI.SfeI (CCWGG+CTRYAG) double digest, 13386 thousand fragments (9855646+3531009 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     176 bp
  25% of sequence in fragments shorter than     217 bp
  30% of sequence in fragments shorter than     259 bp
  35% of sequence in fragments shorter than     303 bp
  40% of sequence in fragments shorter than     349 bp
  45% of sequence in fragments shorter than     400 bp
  50% of sequence in fragments shorter than     456 bp
  55% of sequence in fragments shorter than     516 bp
  60% of sequence in fragments shorter than     582 bp
  65% of sequence in fragments shorter than     655 bp
  70% of sequence in fragments shorter than     737 bp
  75% of sequence in fragments shorter than     840 bp
  80% of sequence in fragments shorter than     970 bp
  85% of sequence in fragments shorter than    1135 bp
  90% of sequence in fragments shorter than    1379 bp
  95% of sequence in fragments shorter than    1810 bp

# BccI.BspKT6I (CCATC+GATC) double digest, 13369 thousand fragments (6170927+7199434 sites), 222 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     120 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     211 bp
  30% of sequence in fragments shorter than     246 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     315 bp
  45% of sequence in fragments shorter than     351 bp
  50% of sequence in fragments shorter than     390 bp
  55% of sequence in fragments shorter than     432 bp
  60% of sequence in fragments shorter than     477 bp
  65% of sequence in fragments shorter than     530 bp
  70% of sequence in fragments shorter than     590 bp
  75% of sequence in fragments shorter than     658 bp
  80% of sequence in fragments shorter than     741 bp
  85% of sequence in fragments shorter than     850 bp
  90% of sequence in fragments shorter than    1007 bp
  95% of sequence in fragments shorter than    1287 bp

# AluI.BstEII (AGCT+GGTNACC) double digest, 13363 thousand fragments (13027778+335865 sites), 223 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     185 bp
  25% of sequence in fragments shorter than     216 bp
  30% of sequence in fragments shorter than     245 bp
  35% of sequence in fragments shorter than     277 bp
  40% of sequence in fragments shorter than     310 bp
  45% of sequence in fragments shorter than     342 bp
  50% of sequence in fragments shorter than     377 bp
  55% of sequence in fragments shorter than     417 bp
  60% of sequence in fragments shorter than     459 bp
  65% of sequence in fragments shorter than     507 bp
  70% of sequence in fragments shorter than     562 bp
  75% of sequence in fragments shorter than     626 bp
  80% of sequence in fragments shorter than     702 bp
  85% of sequence in fragments shorter than     800 bp
  90% of sequence in fragments shorter than     943 bp
  95% of sequence in fragments shorter than    1199 bp

# HgiCI.ScrFI (GGYRCC+CCNGG) double digest, 13355 thousand fragments (1550877+11805099 sites), 223 bp average fragment length
   5% of sequence in fragments shorter than      59 bp
  10% of sequence in fragments shorter than      98 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     187 bp
  25% of sequence in fragments shorter than     237 bp
  30% of sequence in fragments shorter than     291 bp
  35% of sequence in fragments shorter than     348 bp
  40% of sequence in fragments shorter than     409 bp
  45% of sequence in fragments shorter than     478 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     723 bp
  65% of sequence in fragments shorter than     829 bp
  70% of sequence in fragments shorter than     957 bp
  75% of sequence in fragments shorter than    1109 bp
  80% of sequence in fragments shorter than    1295 bp
  85% of sequence in fragments shorter than    1551 bp
  90% of sequence in fragments shorter than    1927 bp
  95% of sequence in fragments shorter than    2685 bp

# BsmI.ScrFI (GAATGC+CCNGG) double digest, 13353 thousand fragments (1549350+11805099 sites), 223 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     102 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     175 bp
  25% of sequence in fragments shorter than     219 bp
  30% of sequence in fragments shorter than     266 bp
  35% of sequence in fragments shorter than     318 bp
  40% of sequence in fragments shorter than     370 bp
  45% of sequence in fragments shorter than     429 bp
  50% of sequence in fragments shorter than     493 bp
  55% of sequence in fragments shorter than     565 bp
  60% of sequence in fragments shorter than     645 bp
  65% of sequence in fragments shorter than     734 bp
  70% of sequence in fragments shorter than     839 bp
  75% of sequence in fragments shorter than     965 bp
  80% of sequence in fragments shorter than    1119 bp
  85% of sequence in fragments shorter than    1320 bp
  90% of sequence in fragments shorter than    1614 bp
  95% of sequence in fragments shorter than    2090 bp

# AluI.SexAI (AGCT+ACCWGGT) double digest, 13325 thousand fragments (13027778+297900 sites), 223 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     185 bp
  25% of sequence in fragments shorter than     216 bp
  30% of sequence in fragments shorter than     246 bp
  35% of sequence in fragments shorter than     278 bp
  40% of sequence in fragments shorter than     311 bp
  45% of sequence in fragments shorter than     343 bp
  50% of sequence in fragments shorter than     379 bp
  55% of sequence in fragments shorter than     419 bp
  60% of sequence in fragments shorter than     461 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     564 bp
  75% of sequence in fragments shorter than     628 bp
  80% of sequence in fragments shorter than     703 bp
  85% of sequence in fragments shorter than     802 bp
  90% of sequence in fragments shorter than     945 bp
  95% of sequence in fragments shorter than    1201 bp

# AluI.KpnI (AGCT+GGTACC) double digest, 13315 thousand fragments (13027778+287974 sites), 223 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     185 bp
  25% of sequence in fragments shorter than     216 bp
  30% of sequence in fragments shorter than     247 bp
  35% of sequence in fragments shorter than     278 bp
  40% of sequence in fragments shorter than     311 bp
  45% of sequence in fragments shorter than     344 bp
  50% of sequence in fragments shorter than     380 bp
  55% of sequence in fragments shorter than     419 bp
  60% of sequence in fragments shorter than     462 bp
  65% of sequence in fragments shorter than     510 bp
  70% of sequence in fragments shorter than     565 bp
  75% of sequence in fragments shorter than     629 bp
  80% of sequence in fragments shorter than     704 bp
  85% of sequence in fragments shorter than     803 bp
  90% of sequence in fragments shorter than     947 bp
  95% of sequence in fragments shorter than    1203 bp

# AluI.NheI (AGCT+GCTAGC) double digest, 13314 thousand fragments (13027778+287384 sites), 223 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     155 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     219 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     281 bp
  40% of sequence in fragments shorter than     314 bp
  45% of sequence in fragments shorter than     347 bp
  50% of sequence in fragments shorter than     383 bp
  55% of sequence in fragments shorter than     424 bp
  60% of sequence in fragments shorter than     468 bp
  65% of sequence in fragments shorter than     516 bp
  70% of sequence in fragments shorter than     572 bp
  75% of sequence in fragments shorter than     637 bp
  80% of sequence in fragments shorter than     712 bp
  85% of sequence in fragments shorter than     813 bp
  90% of sequence in fragments shorter than     958 bp
  95% of sequence in fragments shorter than    1216 bp

# AflIII.ScrFI (ACRYGT+CCNGG) double digest, 13290 thousand fragments (1485394+11805099 sites), 224 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     224 bp
  30% of sequence in fragments shorter than     271 bp
  35% of sequence in fragments shorter than     321 bp
  40% of sequence in fragments shorter than     374 bp
  45% of sequence in fragments shorter than     429 bp
  50% of sequence in fragments shorter than     493 bp
  55% of sequence in fragments shorter than     563 bp
  60% of sequence in fragments shorter than     639 bp
  65% of sequence in fragments shorter than     723 bp
  70% of sequence in fragments shorter than     825 bp
  75% of sequence in fragments shorter than     947 bp
  80% of sequence in fragments shorter than    1098 bp
  85% of sequence in fragments shorter than    1296 bp
  90% of sequence in fragments shorter than    1579 bp
  95% of sequence in fragments shorter than    2092 bp

# BspNCI.HphI (CCAGA+GGTGA) double digest, 13289 thousand fragments (7282436+6007336 sites), 224 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     116 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     182 bp
  25% of sequence in fragments shorter than     216 bp
  30% of sequence in fragments shorter than     250 bp
  35% of sequence in fragments shorter than     285 bp
  40% of sequence in fragments shorter than     319 bp
  45% of sequence in fragments shorter than     354 bp
  50% of sequence in fragments shorter than     394 bp
  55% of sequence in fragments shorter than     438 bp
  60% of sequence in fragments shorter than     486 bp
  65% of sequence in fragments shorter than     537 bp
  70% of sequence in fragments shorter than     592 bp
  75% of sequence in fragments shorter than     661 bp
  80% of sequence in fragments shorter than     744 bp
  85% of sequence in fragments shorter than     856 bp
  90% of sequence in fragments shorter than    1016 bp
  95% of sequence in fragments shorter than    1269 bp

# BthCI.Hin4II (GCNGC+CCTTC) double digest, 13269 thousand fragments (6209929+7059915 sites), 224 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     159 bp
  20% of sequence in fragments shorter than     198 bp
  25% of sequence in fragments shorter than     240 bp
  30% of sequence in fragments shorter than     280 bp
  35% of sequence in fragments shorter than     323 bp
  40% of sequence in fragments shorter than     366 bp
  45% of sequence in fragments shorter than     413 bp
  50% of sequence in fragments shorter than     464 bp
  55% of sequence in fragments shorter than     520 bp
  60% of sequence in fragments shorter than     579 bp
  65% of sequence in fragments shorter than     649 bp
  70% of sequence in fragments shorter than     727 bp
  75% of sequence in fragments shorter than     819 bp
  80% of sequence in fragments shorter than     932 bp
  85% of sequence in fragments shorter than    1076 bp
  90% of sequence in fragments shorter than    1277 bp
  95% of sequence in fragments shorter than    1622 bp

# AluI.BsrBI (AGCT+CCGCTC) double digest, 13262 thousand fragments (13027778+235523 sites), 224 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     116 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     186 bp
  25% of sequence in fragments shorter than     217 bp
  30% of sequence in fragments shorter than     248 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     312 bp
  45% of sequence in fragments shorter than     345 bp
  50% of sequence in fragments shorter than     381 bp
  55% of sequence in fragments shorter than     422 bp
  60% of sequence in fragments shorter than     465 bp
  65% of sequence in fragments shorter than     513 bp
  70% of sequence in fragments shorter than     568 bp
  75% of sequence in fragments shorter than     633 bp
  80% of sequence in fragments shorter than     708 bp
  85% of sequence in fragments shorter than     808 bp
  90% of sequence in fragments shorter than     952 bp
  95% of sequence in fragments shorter than    1209 bp

# AluI.KasI (AGCT+GGCGCC) double digest, 13257 thousand fragments (13027778+230381 sites), 224 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     187 bp
  25% of sequence in fragments shorter than     218 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     281 bp
  40% of sequence in fragments shorter than     315 bp
  45% of sequence in fragments shorter than     347 bp
  50% of sequence in fragments shorter than     384 bp
  55% of sequence in fragments shorter than     424 bp
  60% of sequence in fragments shorter than     467 bp
  65% of sequence in fragments shorter than     516 bp
  70% of sequence in fragments shorter than     572 bp
  75% of sequence in fragments shorter than     637 bp
  80% of sequence in fragments shorter than     712 bp
  85% of sequence in fragments shorter than     813 bp
  90% of sequence in fragments shorter than     958 bp
  95% of sequence in fragments shorter than    1216 bp

# AluI.SwaI (AGCT+ATTTAAAT) double digest, 13253 thousand fragments (13027778+226602 sites), 224 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     116 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     186 bp
  25% of sequence in fragments shorter than     217 bp
  30% of sequence in fragments shorter than     247 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     311 bp
  45% of sequence in fragments shorter than     344 bp
  50% of sequence in fragments shorter than     379 bp
  55% of sequence in fragments shorter than     419 bp
  60% of sequence in fragments shorter than     462 bp
  65% of sequence in fragments shorter than     509 bp
  70% of sequence in fragments shorter than     563 bp
  75% of sequence in fragments shorter than     627 bp
  80% of sequence in fragments shorter than     702 bp
  85% of sequence in fragments shorter than     799 bp
  90% of sequence in fragments shorter than     940 bp
  95% of sequence in fragments shorter than    1193 bp

# FauI.ScrFI (CCCGC+CCNGG) double digest, 13244 thousand fragments (1439772+11805099 sites), 225 bp average fragment length
   5% of sequence in fragments shorter than      57 bp
  10% of sequence in fragments shorter than      98 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     242 bp
  30% of sequence in fragments shorter than     299 bp
  35% of sequence in fragments shorter than     359 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     577 bp
  55% of sequence in fragments shorter than     662 bp
  60% of sequence in fragments shorter than     761 bp
  65% of sequence in fragments shorter than     877 bp
  70% of sequence in fragments shorter than    1013 bp
  75% of sequence in fragments shorter than    1177 bp
  80% of sequence in fragments shorter than    1378 bp
  85% of sequence in fragments shorter than    1647 bp
  90% of sequence in fragments shorter than    2030 bp
  95% of sequence in fragments shorter than    2745 bp

# BspMI.ScrFI (ACCTGC+CCNGG) double digest, 13242 thousand fragments (1438129+11805099 sites), 225 bp average fragment length
   5% of sequence in fragments shorter than      62 bp
  10% of sequence in fragments shorter than      99 bp
  15% of sequence in fragments shorter than     137 bp
  20% of sequence in fragments shorter than     181 bp
  25% of sequence in fragments shorter than     230 bp
  30% of sequence in fragments shorter than     281 bp
  35% of sequence in fragments shorter than     334 bp
  40% of sequence in fragments shorter than     389 bp
  45% of sequence in fragments shorter than     455 bp
  50% of sequence in fragments shorter than     525 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     689 bp
  65% of sequence in fragments shorter than     789 bp
  70% of sequence in fragments shorter than     903 bp
  75% of sequence in fragments shorter than    1043 bp
  80% of sequence in fragments shorter than    1219 bp
  85% of sequence in fragments shorter than    1444 bp
  90% of sequence in fragments shorter than    1786 bp
  95% of sequence in fragments shorter than    2311 bp

# BccI.Hin4II (CCATC+CCTTC) double digest, 13230 thousand fragments (6170927+7059915 sites), 225 bp average fragment length
   5% of sequence in fragments shorter than      77 bp
  10% of sequence in fragments shorter than     119 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     191 bp
  25% of sequence in fragments shorter than     224 bp
  30% of sequence in fragments shorter than     258 bp
  35% of sequence in fragments shorter than     294 bp
  40% of sequence in fragments shorter than     331 bp
  45% of sequence in fragments shorter than     368 bp
  50% of sequence in fragments shorter than     410 bp
  55% of sequence in fragments shorter than     455 bp
  60% of sequence in fragments shorter than     506 bp
  65% of sequence in fragments shorter than     561 bp
  70% of sequence in fragments shorter than     625 bp
  75% of sequence in fragments shorter than     699 bp
  80% of sequence in fragments shorter than     789 bp
  85% of sequence in fragments shorter than     905 bp
  90% of sequence in fragments shorter than    1067 bp
  95% of sequence in fragments shorter than    1351 bp

# MboII.Tsp45I (GAAGA+GTSAC) double digest, 13220 thousand fragments (9199496+4021761 sites), 225 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     184 bp
  25% of sequence in fragments shorter than     218 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     287 bp
  40% of sequence in fragments shorter than     322 bp
  45% of sequence in fragments shorter than     357 bp
  50% of sequence in fragments shorter than     397 bp
  55% of sequence in fragments shorter than     438 bp
  60% of sequence in fragments shorter than     485 bp
  65% of sequence in fragments shorter than     536 bp
  70% of sequence in fragments shorter than     594 bp
  75% of sequence in fragments shorter than     661 bp
  80% of sequence in fragments shorter than     742 bp
  85% of sequence in fragments shorter than     842 bp
  90% of sequence in fragments shorter than     984 bp
  95% of sequence in fragments shorter than    1210 bp

# BspKT6I.HphI (GATC+GGTGA) double digest, 13206 thousand fragments (7199434+6007336 sites), 225 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     155 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     218 bp
  30% of sequence in fragments shorter than     254 bp
  35% of sequence in fragments shorter than     289 bp
  40% of sequence in fragments shorter than     325 bp
  45% of sequence in fragments shorter than     360 bp
  50% of sequence in fragments shorter than     401 bp
  55% of sequence in fragments shorter than     445 bp
  60% of sequence in fragments shorter than     490 bp
  65% of sequence in fragments shorter than     541 bp
  70% of sequence in fragments shorter than     601 bp
  75% of sequence in fragments shorter than     670 bp
  80% of sequence in fragments shorter than     750 bp
  85% of sequence in fragments shorter than     854 bp
  90% of sequence in fragments shorter than    1006 bp
  95% of sequence in fragments shorter than    1256 bp

# AclI.AluI (AACGTT+AGCT) double digest, 13199 thousand fragments (172706+13027778 sites), 225 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     187 bp
  25% of sequence in fragments shorter than     218 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     280 bp
  40% of sequence in fragments shorter than     313 bp
  45% of sequence in fragments shorter than     346 bp
  50% of sequence in fragments shorter than     382 bp
  55% of sequence in fragments shorter than     422 bp
  60% of sequence in fragments shorter than     465 bp
  65% of sequence in fragments shorter than     512 bp
  70% of sequence in fragments shorter than     567 bp
  75% of sequence in fragments shorter than     632 bp
  80% of sequence in fragments shorter than     707 bp
  85% of sequence in fragments shorter than     806 bp
  90% of sequence in fragments shorter than     948 bp
  95% of sequence in fragments shorter than    1194 bp

# AluI.PacI (AGCT+TTAATTAA) double digest, 13186 thousand fragments (13027778+159455 sites), 225 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     155 bp
  20% of sequence in fragments shorter than     187 bp
  25% of sequence in fragments shorter than     218 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     280 bp
  40% of sequence in fragments shorter than     314 bp
  45% of sequence in fragments shorter than     346 bp
  50% of sequence in fragments shorter than     382 bp
  55% of sequence in fragments shorter than     423 bp
  60% of sequence in fragments shorter than     465 bp
  65% of sequence in fragments shorter than     513 bp
  70% of sequence in fragments shorter than     568 bp
  75% of sequence in fragments shorter than     633 bp
  80% of sequence in fragments shorter than     707 bp
  85% of sequence in fragments shorter than     807 bp
  90% of sequence in fragments shorter than     949 bp
  95% of sequence in fragments shorter than    1205 bp

# AluI.Hpy99I (AGCT+CGWCG) double digest, 13185 thousand fragments (13027778+157917 sites), 226 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     187 bp
  25% of sequence in fragments shorter than     218 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     281 bp
  40% of sequence in fragments shorter than     314 bp
  45% of sequence in fragments shorter than     347 bp
  50% of sequence in fragments shorter than     383 bp
  55% of sequence in fragments shorter than     424 bp
  60% of sequence in fragments shorter than     467 bp
  65% of sequence in fragments shorter than     515 bp
  70% of sequence in fragments shorter than     571 bp
  75% of sequence in fragments shorter than     636 bp
  80% of sequence in fragments shorter than     711 bp
  85% of sequence in fragments shorter than     812 bp
  90% of sequence in fragments shorter than     957 bp
  95% of sequence in fragments shorter than    1215 bp

# AluI.SnaBI (AGCT+TACGTA) double digest, 13159 thousand fragments (13027778+132299 sites), 226 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     155 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     219 bp
  30% of sequence in fragments shorter than     249 bp
  35% of sequence in fragments shorter than     281 bp
  40% of sequence in fragments shorter than     314 bp
  45% of sequence in fragments shorter than     347 bp
  50% of sequence in fragments shorter than     383 bp
  55% of sequence in fragments shorter than     424 bp
  60% of sequence in fragments shorter than     466 bp
  65% of sequence in fragments shorter than     514 bp
  70% of sequence in fragments shorter than     569 bp
  75% of sequence in fragments shorter than     634 bp
  80% of sequence in fragments shorter than     709 bp
  85% of sequence in fragments shorter than     809 bp
  90% of sequence in fragments shorter than     952 bp
  95% of sequence in fragments shorter than    1207 bp

# AluI.NaeI (AGCT+GCCGGC) double digest, 13152 thousand fragments (13027778+125692 sites), 226 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     155 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     219 bp
  30% of sequence in fragments shorter than     250 bp
  35% of sequence in fragments shorter than     282 bp
  40% of sequence in fragments shorter than     315 bp
  45% of sequence in fragments shorter than     347 bp
  50% of sequence in fragments shorter than     384 bp
  55% of sequence in fragments shorter than     425 bp
  60% of sequence in fragments shorter than     468 bp
  65% of sequence in fragments shorter than     517 bp
  70% of sequence in fragments shorter than     572 bp
  75% of sequence in fragments shorter than     638 bp
  80% of sequence in fragments shorter than     713 bp
  85% of sequence in fragments shorter than     815 bp
  90% of sequence in fragments shorter than     960 bp
  95% of sequence in fragments shorter than    1219 bp

# AluI.XhoI (AGCT+CTCGAG) double digest, 13148 thousand fragments (13027778+121308 sites), 226 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     155 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     219 bp
  30% of sequence in fragments shorter than     250 bp
  35% of sequence in fragments shorter than     282 bp
  40% of sequence in fragments shorter than     315 bp
  45% of sequence in fragments shorter than     347 bp
  50% of sequence in fragments shorter than     384 bp
  55% of sequence in fragments shorter than     425 bp
  60% of sequence in fragments shorter than     468 bp
  65% of sequence in fragments shorter than     517 bp
  70% of sequence in fragments shorter than     572 bp
  75% of sequence in fragments shorter than     638 bp
  80% of sequence in fragments shorter than     714 bp
  85% of sequence in fragments shorter than     815 bp
  90% of sequence in fragments shorter than     961 bp
  95% of sequence in fragments shorter than    1220 bp

# AciI.Hpy188I (CCGC+TCNGA) double digest, 13147 thousand fragments (4206130+8942153 sites), 226 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     157 bp
  20% of sequence in fragments shorter than     187 bp
  25% of sequence in fragments shorter than     224 bp
  30% of sequence in fragments shorter than     261 bp
  35% of sequence in fragments shorter than     298 bp
  40% of sequence in fragments shorter than     337 bp
  45% of sequence in fragments shorter than     375 bp
  50% of sequence in fragments shorter than     419 bp
  55% of sequence in fragments shorter than     465 bp
  60% of sequence in fragments shorter than     516 bp
  65% of sequence in fragments shorter than     573 bp
  70% of sequence in fragments shorter than     636 bp
  75% of sequence in fragments shorter than     711 bp
  80% of sequence in fragments shorter than     801 bp
  85% of sequence in fragments shorter than     915 bp
  90% of sequence in fragments shorter than    1072 bp
  95% of sequence in fragments shorter than    1322 bp

# MboII.MlyI (GAAGA+GAGTC) double digest, 13130 thousand fragments (9199496+3931692 sites), 226 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     122 bp
  15% of sequence in fragments shorter than     157 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     224 bp
  30% of sequence in fragments shorter than     259 bp
  35% of sequence in fragments shorter than     294 bp
  40% of sequence in fragments shorter than     327 bp
  45% of sequence in fragments shorter than     362 bp
  50% of sequence in fragments shorter than     402 bp
  55% of sequence in fragments shorter than     442 bp
  60% of sequence in fragments shorter than     488 bp
  65% of sequence in fragments shorter than     538 bp
  70% of sequence in fragments shorter than     596 bp
  75% of sequence in fragments shorter than     661 bp
  80% of sequence in fragments shorter than     741 bp
  85% of sequence in fragments shorter than     840 bp
  90% of sequence in fragments shorter than     974 bp
  95% of sequence in fragments shorter than    1199 bp

# PstI.ScrFI (CTGCAG+CCNGG) double digest, 13126 thousand fragments (1321469+11805099 sites), 227 bp average fragment length
   5% of sequence in fragments shorter than      63 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     138 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     233 bp
  30% of sequence in fragments shorter than     285 bp
  35% of sequence in fragments shorter than     341 bp
  40% of sequence in fragments shorter than     402 bp
  45% of sequence in fragments shorter than     470 bp
  50% of sequence in fragments shorter than     543 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     710 bp
  65% of sequence in fragments shorter than     817 bp
  70% of sequence in fragments shorter than     942 bp
  75% of sequence in fragments shorter than    1094 bp
  80% of sequence in fragments shorter than    1282 bp
  85% of sequence in fragments shorter than    1534 bp
  90% of sequence in fragments shorter than    1900 bp
  95% of sequence in fragments shorter than    2564 bp

# AciI.HinfI (CCGC+GANTC) double digest, 13121 thousand fragments (4206130+8916054 sites), 227 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     155 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     227 bp
  30% of sequence in fragments shorter than     266 bp
  35% of sequence in fragments shorter than     304 bp
  40% of sequence in fragments shorter than     342 bp
  45% of sequence in fragments shorter than     379 bp
  50% of sequence in fragments shorter than     425 bp
  55% of sequence in fragments shorter than     474 bp
  60% of sequence in fragments shorter than     528 bp
  65% of sequence in fragments shorter than     588 bp
  70% of sequence in fragments shorter than     656 bp
  75% of sequence in fragments shorter than     731 bp
  80% of sequence in fragments shorter than     822 bp
  85% of sequence in fragments shorter than     941 bp
  90% of sequence in fragments shorter than    1105 bp
  95% of sequence in fragments shorter than    1385 bp

# AluI.ClaI (AGCT+ATCGAT) double digest, 13113 thousand fragments (13027778+86477 sites), 227 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     155 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     219 bp
  30% of sequence in fragments shorter than     250 bp
  35% of sequence in fragments shorter than     282 bp
  40% of sequence in fragments shorter than     316 bp
  45% of sequence in fragments shorter than     348 bp
  50% of sequence in fragments shorter than     385 bp
  55% of sequence in fragments shorter than     426 bp
  60% of sequence in fragments shorter than     468 bp
  65% of sequence in fragments shorter than     517 bp
  70% of sequence in fragments shorter than     572 bp
  75% of sequence in fragments shorter than     638 bp
  80% of sequence in fragments shorter than     713 bp
  85% of sequence in fragments shorter than     814 bp
  90% of sequence in fragments shorter than     958 bp
  95% of sequence in fragments shorter than    1216 bp

# AatII.AluI (GACGTC+AGCT) double digest, 13098 thousand fragments (71089+13027778 sites), 227 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     220 bp
  30% of sequence in fragments shorter than     251 bp
  35% of sequence in fragments shorter than     283 bp
  40% of sequence in fragments shorter than     316 bp
  45% of sequence in fragments shorter than     348 bp
  50% of sequence in fragments shorter than     385 bp
  55% of sequence in fragments shorter than     426 bp
  60% of sequence in fragments shorter than     469 bp
  65% of sequence in fragments shorter than     518 bp
  70% of sequence in fragments shorter than     574 bp
  75% of sequence in fragments shorter than     639 bp
  80% of sequence in fragments shorter than     715 bp
  85% of sequence in fragments shorter than     816 bp
  90% of sequence in fragments shorter than     961 bp
  95% of sequence in fragments shorter than    1220 bp

# AluI.SacII (AGCT+CCGCGG) double digest, 13094 thousand fragments (13027778+66901 sites), 227 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     220 bp
  30% of sequence in fragments shorter than     251 bp
  35% of sequence in fragments shorter than     283 bp
  40% of sequence in fragments shorter than     316 bp
  45% of sequence in fragments shorter than     348 bp
  50% of sequence in fragments shorter than     386 bp
  55% of sequence in fragments shorter than     427 bp
  60% of sequence in fragments shorter than     469 bp
  65% of sequence in fragments shorter than     518 bp
  70% of sequence in fragments shorter than     574 bp
  75% of sequence in fragments shorter than     640 bp
  80% of sequence in fragments shorter than     715 bp
  85% of sequence in fragments shorter than     817 bp
  90% of sequence in fragments shorter than     962 bp
  95% of sequence in fragments shorter than    1222 bp

# AluI.PspXI (AGCT+VCTCGAGB) double digest, 13086 thousand fragments (13027778+59193 sites), 227 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     220 bp
  30% of sequence in fragments shorter than     251 bp
  35% of sequence in fragments shorter than     283 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     349 bp
  50% of sequence in fragments shorter than     386 bp
  55% of sequence in fragments shorter than     427 bp
  60% of sequence in fragments shorter than     470 bp
  65% of sequence in fragments shorter than     519 bp
  70% of sequence in fragments shorter than     575 bp
  75% of sequence in fragments shorter than     640 bp
  80% of sequence in fragments shorter than     716 bp
  85% of sequence in fragments shorter than     818 bp
  90% of sequence in fragments shorter than     963 bp
  95% of sequence in fragments shorter than    1223 bp

# AgeI.AluI (ACCGGT+AGCT) double digest, 13080 thousand fragments (53386+13027778 sites), 227 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     220 bp
  30% of sequence in fragments shorter than     251 bp
  35% of sequence in fragments shorter than     283 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     349 bp
  50% of sequence in fragments shorter than     386 bp
  55% of sequence in fragments shorter than     427 bp
  60% of sequence in fragments shorter than     470 bp
  65% of sequence in fragments shorter than     518 bp
  70% of sequence in fragments shorter than     574 bp
  75% of sequence in fragments shorter than     640 bp
  80% of sequence in fragments shorter than     715 bp
  85% of sequence in fragments shorter than     817 bp
  90% of sequence in fragments shorter than     962 bp
  95% of sequence in fragments shorter than    1221 bp

# AluI.PmeI (AGCT+GTTTAAAC) double digest, 13069 thousand fragments (13027778+42094 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     220 bp
  30% of sequence in fragments shorter than     251 bp
  35% of sequence in fragments shorter than     283 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     349 bp
  50% of sequence in fragments shorter than     386 bp
  55% of sequence in fragments shorter than     427 bp
  60% of sequence in fragments shorter than     470 bp
  65% of sequence in fragments shorter than     519 bp
  70% of sequence in fragments shorter than     574 bp
  75% of sequence in fragments shorter than     640 bp
  80% of sequence in fragments shorter than     715 bp
  85% of sequence in fragments shorter than     816 bp
  90% of sequence in fragments shorter than     962 bp
  95% of sequence in fragments shorter than    1221 bp

# Hin4II.HphI (CCTTC+GGTGA) double digest, 13066 thousand fragments (7059915+6007336 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      79 bp
  10% of sequence in fragments shorter than     122 bp
  15% of sequence in fragments shorter than     160 bp
  20% of sequence in fragments shorter than     194 bp
  25% of sequence in fragments shorter than     229 bp
  30% of sequence in fragments shorter than     264 bp
  35% of sequence in fragments shorter than     299 bp
  40% of sequence in fragments shorter than     336 bp
  45% of sequence in fragments shorter than     371 bp
  50% of sequence in fragments shorter than     413 bp
  55% of sequence in fragments shorter than     457 bp
  60% of sequence in fragments shorter than     507 bp
  65% of sequence in fragments shorter than     556 bp
  70% of sequence in fragments shorter than     618 bp
  75% of sequence in fragments shorter than     690 bp
  80% of sequence in fragments shorter than     778 bp
  85% of sequence in fragments shorter than     890 bp
  90% of sequence in fragments shorter than    1046 bp
  95% of sequence in fragments shorter than    1317 bp

# AluI.SalI (AGCT+GTCGAC) double digest, 13058 thousand fragments (13027778+31396 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     220 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     283 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     349 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     470 bp
  65% of sequence in fragments shorter than     519 bp
  70% of sequence in fragments shorter than     575 bp
  75% of sequence in fragments shorter than     641 bp
  80% of sequence in fragments shorter than     717 bp
  85% of sequence in fragments shorter than     818 bp
  90% of sequence in fragments shorter than     964 bp
  95% of sequence in fragments shorter than    1224 bp

# AluI.MluI (AGCT+ACGCGT) double digest, 13048 thousand fragments (13027778+21415 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     349 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     641 bp
  80% of sequence in fragments shorter than     717 bp
  85% of sequence in fragments shorter than     819 bp
  90% of sequence in fragments shorter than     964 bp
  95% of sequence in fragments shorter than    1224 bp

# AluI.SgrAI (AGCT+CRCCGGYG) double digest, 13042 thousand fragments (13027778+15270 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     717 bp
  85% of sequence in fragments shorter than     819 bp
  90% of sequence in fragments shorter than     965 bp
  95% of sequence in fragments shorter than    1225 bp

# AluI.NruI (AGCT+TCGCGA) double digest, 13042 thousand fragments (13027778+14872 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     318 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     819 bp
  90% of sequence in fragments shorter than     965 bp
  95% of sequence in fragments shorter than    1225 bp

# AluI.PvuI (AGCT+CGATCG) double digest, 13041 thousand fragments (13027778+14186 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     317 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     641 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     819 bp
  90% of sequence in fragments shorter than     965 bp
  95% of sequence in fragments shorter than    1225 bp

# AluI.FseI (AGCT+GGCCGGCC) double digest, 13040 thousand fragments (13027778+13662 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     318 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     819 bp
  90% of sequence in fragments shorter than     965 bp
  95% of sequence in fragments shorter than    1225 bp

# AluI.NotI (AGCT+GCGGCCGC) double digest, 13037 thousand fragments (13027778+9795 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     318 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     819 bp
  90% of sequence in fragments shorter than     965 bp
  95% of sequence in fragments shorter than    1225 bp

# AluI.RsrII (AGCT+CGGWCCG) double digest, 13037 thousand fragments (13027778+9764 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     318 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     820 bp
  90% of sequence in fragments shorter than     965 bp
  95% of sequence in fragments shorter than    1225 bp

# BscGI.ScrFI (CCCGT+CCNGG) double digest, 13035 thousand fragments (1231070+11805099 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     100 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     187 bp
  25% of sequence in fragments shorter than     238 bp
  30% of sequence in fragments shorter than     294 bp
  35% of sequence in fragments shorter than     345 bp
  40% of sequence in fragments shorter than     402 bp
  45% of sequence in fragments shorter than     471 bp
  50% of sequence in fragments shorter than     545 bp
  55% of sequence in fragments shorter than     627 bp
  60% of sequence in fragments shorter than     714 bp
  65% of sequence in fragments shorter than     821 bp
  70% of sequence in fragments shorter than     944 bp
  75% of sequence in fragments shorter than    1092 bp
  80% of sequence in fragments shorter than    1275 bp
  85% of sequence in fragments shorter than    1518 bp
  90% of sequence in fragments shorter than    1864 bp
  95% of sequence in fragments shorter than    2439 bp

# AluI.AscI (AGCT+GGCGCGCC) double digest, 13032 thousand fragments (13027778+4751 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     252 bp
  35% of sequence in fragments shorter than     284 bp
  40% of sequence in fragments shorter than     318 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     387 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     471 bp
  65% of sequence in fragments shorter than     520 bp
  70% of sequence in fragments shorter than     576 bp
  75% of sequence in fragments shorter than     642 bp
  80% of sequence in fragments shorter than     718 bp
  85% of sequence in fragments shorter than     820 bp
  90% of sequence in fragments shorter than     966 bp
  95% of sequence in fragments shorter than    1226 bp

# BspNCI.BsrI (CCAGA+ACTGG) double digest, 13023 thousand fragments (7282436+5741310 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     186 bp
  25% of sequence in fragments shorter than     221 bp
  30% of sequence in fragments shorter than     256 bp
  35% of sequence in fragments shorter than     293 bp
  40% of sequence in fragments shorter than     330 bp
  45% of sequence in fragments shorter than     367 bp
  50% of sequence in fragments shorter than     410 bp
  55% of sequence in fragments shorter than     456 bp
  60% of sequence in fragments shorter than     506 bp
  65% of sequence in fragments shorter than     561 bp
  70% of sequence in fragments shorter than     623 bp
  75% of sequence in fragments shorter than     693 bp
  80% of sequence in fragments shorter than     782 bp
  85% of sequence in fragments shorter than     892 bp
  90% of sequence in fragments shorter than    1046 bp
  95% of sequence in fragments shorter than    1322 bp

# ApoI.BsmAI (RAATTY+GTCTC) double digest, 13013 thousand fragments (6382433+6631585 sites), 228 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     122 bp
  15% of sequence in fragments shorter than     157 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     213 bp
  30% of sequence in fragments shorter than     245 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     314 bp
  45% of sequence in fragments shorter than     350 bp
  50% of sequence in fragments shorter than     389 bp
  55% of sequence in fragments shorter than     431 bp
  60% of sequence in fragments shorter than     478 bp
  65% of sequence in fragments shorter than     531 bp
  70% of sequence in fragments shorter than     590 bp
  75% of sequence in fragments shorter than     658 bp
  80% of sequence in fragments shorter than     743 bp
  85% of sequence in fragments shorter than     852 bp
  90% of sequence in fragments shorter than     994 bp
  95% of sequence in fragments shorter than    1260 bp

# AciI.TspRI (CCGC+CASTG) double digest, 12970 thousand fragments (4206130+8765240 sites), 229 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     165 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     235 bp
  30% of sequence in fragments shorter than     277 bp
  35% of sequence in fragments shorter than     320 bp
  40% of sequence in fragments shorter than     364 bp
  45% of sequence in fragments shorter than     412 bp
  50% of sequence in fragments shorter than     464 bp
  55% of sequence in fragments shorter than     520 bp
  60% of sequence in fragments shorter than     580 bp
  65% of sequence in fragments shorter than     649 bp
  70% of sequence in fragments shorter than     726 bp
  75% of sequence in fragments shorter than     818 bp
  80% of sequence in fragments shorter than     930 bp
  85% of sequence in fragments shorter than    1070 bp
  90% of sequence in fragments shorter than    1269 bp
  95% of sequence in fragments shorter than    1611 bp

# BstNI.StyI (CCWGG+CCWWGG) double digest, 12969 thousand fragments (9855646+3114107 sites), 229 bp average fragment length
   5% of sequence in fragments shorter than      65 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     177 bp
  25% of sequence in fragments shorter than     219 bp
  30% of sequence in fragments shorter than     264 bp
  35% of sequence in fragments shorter than     310 bp
  40% of sequence in fragments shorter than     360 bp
  45% of sequence in fragments shorter than     416 bp
  50% of sequence in fragments shorter than     476 bp
  55% of sequence in fragments shorter than     542 bp
  60% of sequence in fragments shorter than     615 bp
  65% of sequence in fragments shorter than     697 bp
  70% of sequence in fragments shorter than     788 bp
  75% of sequence in fragments shorter than     908 bp
  80% of sequence in fragments shorter than    1057 bp
  85% of sequence in fragments shorter than    1257 bp
  90% of sequence in fragments shorter than    1546 bp
  95% of sequence in fragments shorter than    2124 bp

# Hpy188I.Tsp45I (TCNGA+GTSAC) double digest, 12963 thousand fragments (8942153+4021761 sites), 229 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     183 bp
  25% of sequence in fragments shorter than     216 bp
  30% of sequence in fragments shorter than     250 bp
  35% of sequence in fragments shorter than     286 bp
  40% of sequence in fragments shorter than     321 bp
  45% of sequence in fragments shorter than     355 bp
  50% of sequence in fragments shorter than     395 bp
  55% of sequence in fragments shorter than     437 bp
  60% of sequence in fragments shorter than     483 bp
  65% of sequence in fragments shorter than     535 bp
  70% of sequence in fragments shorter than     594 bp
  75% of sequence in fragments shorter than     662 bp
  80% of sequence in fragments shorter than     746 bp
  85% of sequence in fragments shorter than     849 bp
  90% of sequence in fragments shorter than     994 bp
  95% of sequence in fragments shorter than    1232 bp

# BstNI.NspI (CCWGG+RCATGY) double digest, 12959 thousand fragments (9855646+3104359 sites), 229 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     179 bp
  25% of sequence in fragments shorter than     219 bp
  30% of sequence in fragments shorter than     259 bp
  35% of sequence in fragments shorter than     302 bp
  40% of sequence in fragments shorter than     346 bp
  45% of sequence in fragments shorter than     394 bp
  50% of sequence in fragments shorter than     446 bp
  55% of sequence in fragments shorter than     504 bp
  60% of sequence in fragments shorter than     569 bp
  65% of sequence in fragments shorter than     640 bp
  70% of sequence in fragments shorter than     722 bp
  75% of sequence in fragments shorter than     823 bp
  80% of sequence in fragments shorter than     948 bp
  85% of sequence in fragments shorter than    1111 bp
  90% of sequence in fragments shorter than    1356 bp
  95% of sequence in fragments shorter than    1794 bp

# BspKT6I.BsrI (GATC+ACTGG) double digest, 12940 thousand fragments (7199434+5741310 sites), 230 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     123 bp
  15% of sequence in fragments shorter than     156 bp
  20% of sequence in fragments shorter than     180 bp
  25% of sequence in fragments shorter than     212 bp
  30% of sequence in fragments shorter than     247 bp
  35% of sequence in fragments shorter than     279 bp
  40% of sequence in fragments shorter than     314 bp
  45% of sequence in fragments shorter than     349 bp
  50% of sequence in fragments shorter than     388 bp
  55% of sequence in fragments shorter than     428 bp
  60% of sequence in fragments shorter than     473 bp
  65% of sequence in fragments shorter than     523 bp
  70% of sequence in fragments shorter than     580 bp
  75% of sequence in fragments shorter than     647 bp
  80% of sequence in fragments shorter than     724 bp
  85% of sequence in fragments shorter than     827 bp
  90% of sequence in fragments shorter than     972 bp
  95% of sequence in fragments shorter than    1235 bp

# HinfI.Tsp45I (GANTC+GTSAC) double digest, 12937 thousand fragments (8916054+4021761 sites), 230 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     121 bp
  15% of sequence in fragments shorter than     160 bp
  20% of sequence in fragments shorter than     193 bp
  25% of sequence in fragments shorter than     228 bp
  30% of sequence in fragments shorter than     260 bp
  35% of sequence in fragments shorter than     294 bp
  40% of sequence in fragments shorter than     326 bp
  45% of sequence in fragments shorter than     358 bp
  50% of sequence in fragments shorter than     395 bp
  55% of sequence in fragments shorter than     438 bp
  60% of sequence in fragments shorter than     482 bp
  65% of sequence in fragments shorter than     533 bp
  70% of sequence in fragments shorter than     590 bp
  75% of sequence in fragments shorter than     656 bp
  80% of sequence in fragments shorter than     730 bp
  85% of sequence in fragments shorter than     829 bp
  90% of sequence in fragments shorter than     967 bp
  95% of sequence in fragments shorter than    1197 bp

# AccI.ScrFI (GTMKAC+CCNGG) double digest, 12905 thousand fragments (1101063+11805099 sites), 230 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     141 bp
  20% of sequence in fragments shorter than     186 bp
  25% of sequence in fragments shorter than     232 bp
  30% of sequence in fragments shorter than     282 bp
  35% of sequence in fragments shorter than     333 bp
  40% of sequence in fragments shorter than     389 bp
  45% of sequence in fragments shorter than     450 bp
  50% of sequence in fragments shorter than     517 bp
  55% of sequence in fragments shorter than     590 bp
  60% of sequence in fragments shorter than     668 bp
  65% of sequence in fragments shorter than     759 bp
  70% of sequence in fragments shorter than     867 bp
  75% of sequence in fragments shorter than     996 bp
  80% of sequence in fragments shorter than    1155 bp
  85% of sequence in fragments shorter than    1364 bp
  90% of sequence in fragments shorter than    1670 bp
  95% of sequence in fragments shorter than    2182 bp

# PpuMI.ScrFI (RGGWCCY+CCNGG) double digest, 12889 thousand fragments (1085138+11805099 sites), 231 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     241 bp
  30% of sequence in fragments shorter than     295 bp
  35% of sequence in fragments shorter than     351 bp
  40% of sequence in fragments shorter than     413 bp
  45% of sequence in fragments shorter than     482 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     642 bp
  60% of sequence in fragments shorter than     732 bp
  65% of sequence in fragments shorter than     842 bp
  70% of sequence in fragments shorter than     972 bp
  75% of sequence in fragments shorter than    1129 bp
  80% of sequence in fragments shorter than    1326 bp
  85% of sequence in fragments shorter than    1584 bp
  90% of sequence in fragments shorter than    1974 bp
  95% of sequence in fragments shorter than    2748 bp

# PvuII.ScrFI (CAGCTG+CCNGG) double digest, 12888 thousand fragments (1084260+11805099 sites), 231 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     101 bp
  15% of sequence in fragments shorter than     139 bp
  20% of sequence in fragments shorter than     185 bp
  25% of sequence in fragments shorter than     235 bp
  30% of sequence in fragments shorter than     288 bp
  35% of sequence in fragments shorter than     345 bp
  40% of sequence in fragments shorter than     406 bp
  45% of sequence in fragments shorter than     475 bp
  50% of sequence in fragments shorter than     550 bp
  55% of sequence in fragments shorter than     632 bp
  60% of sequence in fragments shorter than     721 bp
  65% of sequence in fragments shorter than     829 bp
  70% of sequence in fragments shorter than     958 bp
  75% of sequence in fragments shorter than    1113 bp
  80% of sequence in fragments shorter than    1309 bp
  85% of sequence in fragments shorter than    1572 bp
  90% of sequence in fragments shorter than    1955 bp
  95% of sequence in fragments shorter than    2683 bp

# Hpy188I.MlyI (TCNGA+GAGTC) double digest, 12873 thousand fragments (8942153+3931692 sites), 231 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     123 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     191 bp
  25% of sequence in fragments shorter than     226 bp
  30% of sequence in fragments shorter than     261 bp
  35% of sequence in fragments shorter than     297 bp
  40% of sequence in fragments shorter than     333 bp
  45% of sequence in fragments shorter than     366 bp
  50% of sequence in fragments shorter than     406 bp
  55% of sequence in fragments shorter than     449 bp
  60% of sequence in fragments shorter than     496 bp
  65% of sequence in fragments shorter than     549 bp
  70% of sequence in fragments shorter than     608 bp
  75% of sequence in fragments shorter than     678 bp
  80% of sequence in fragments shorter than     761 bp
  85% of sequence in fragments shorter than     866 bp
  90% of sequence in fragments shorter than    1011 bp
  95% of sequence in fragments shorter than    1247 bp

# BscAI.MboII (GCATC+GAAGA) double digest, 12851 thousand fragments (3652926+9199496 sites), 231 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     123 bp
  15% of sequence in fragments shorter than     158 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     226 bp
  30% of sequence in fragments shorter than     262 bp
  35% of sequence in fragments shorter than     300 bp
  40% of sequence in fragments shorter than     336 bp
  45% of sequence in fragments shorter than     374 bp
  50% of sequence in fragments shorter than     418 bp
  55% of sequence in fragments shorter than     465 bp
  60% of sequence in fragments shorter than     516 bp
  65% of sequence in fragments shorter than     571 bp
  70% of sequence in fragments shorter than     635 bp
  75% of sequence in fragments shorter than     709 bp
  80% of sequence in fragments shorter than     799 bp
  85% of sequence in fragments shorter than     911 bp
  90% of sequence in fragments shorter than    1068 bp
  95% of sequence in fragments shorter than    1337 bp

# BsmAI.BthCI (GTCTC+GCNGC) double digest, 12841 thousand fragments (6631585+6209929 sites), 232 bp average fragment length
   5% of sequence in fragments shorter than      76 bp
  10% of sequence in fragments shorter than     121 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     194 bp
  25% of sequence in fragments shorter than     234 bp
  30% of sequence in fragments shorter than     276 bp
  35% of sequence in fragments shorter than     319 bp
  40% of sequence in fragments shorter than     364 bp
  45% of sequence in fragments shorter than     414 bp
  50% of sequence in fragments shorter than     466 bp
  55% of sequence in fragments shorter than     521 bp
  60% of sequence in fragments shorter than     581 bp
  65% of sequence in fragments shorter than     651 bp
  70% of sequence in fragments shorter than     733 bp
  75% of sequence in fragments shorter than     830 bp
  80% of sequence in fragments shorter than     943 bp
  85% of sequence in fragments shorter than    1090 bp
  90% of sequence in fragments shorter than    1299 bp
  95% of sequence in fragments shorter than    1665 bp

# AvaI.ScrFI (CYCGRG+CCNGG) double digest, 12810 thousand fragments (1005714+11805099 sites), 232 bp average fragment length
   5% of sequence in fragments shorter than      64 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     198 bp
  25% of sequence in fragments shorter than     252 bp
  30% of sequence in fragments shorter than     310 bp
  35% of sequence in fragments shorter than     371 bp
  40% of sequence in fragments shorter than     438 bp
  45% of sequence in fragments shorter than     512 bp
  50% of sequence in fragments shorter than     595 bp
  55% of sequence in fragments shorter than     682 bp
  60% of sequence in fragments shorter than     783 bp
  65% of sequence in fragments shorter than     902 bp
  70% of sequence in fragments shorter than    1041 bp
  75% of sequence in fragments shorter than    1213 bp
  80% of sequence in fragments shorter than    1425 bp
  85% of sequence in fragments shorter than    1715 bp
  90% of sequence in fragments shorter than    2129 bp
  95% of sequence in fragments shorter than    2934 bp

# BccI.BsmAI (CCATC+GTCTC) double digest, 12802 thousand fragments (6170927+6631585 sites), 232 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     127 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     224 bp
  30% of sequence in fragments shorter than     262 bp
  35% of sequence in fragments shorter than     301 bp
  40% of sequence in fragments shorter than     340 bp
  45% of sequence in fragments shorter than     382 bp
  50% of sequence in fragments shorter than     427 bp
  55% of sequence in fragments shorter than     476 bp
  60% of sequence in fragments shorter than     531 bp
  65% of sequence in fragments shorter than     592 bp
  70% of sequence in fragments shorter than     662 bp
  75% of sequence in fragments shorter than     745 bp
  80% of sequence in fragments shorter than     845 bp
  85% of sequence in fragments shorter than     978 bp
  90% of sequence in fragments shorter than    1170 bp
  95% of sequence in fragments shorter than    1517 bp

# BsrI.Hin4II (ACTGG+CCTTC) double digest, 12800 thousand fragments (5741310+7059915 sites), 232 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     121 bp
  15% of sequence in fragments shorter than     159 bp
  20% of sequence in fragments shorter than     195 bp
  25% of sequence in fragments shorter than     229 bp
  30% of sequence in fragments shorter than     266 bp
  35% of sequence in fragments shorter than     305 bp
  40% of sequence in fragments shorter than     343 bp
  45% of sequence in fragments shorter than     382 bp
  50% of sequence in fragments shorter than     427 bp
  55% of sequence in fragments shorter than     475 bp
  60% of sequence in fragments shorter than     526 bp
  65% of sequence in fragments shorter than     584 bp
  70% of sequence in fragments shorter than     652 bp
  75% of sequence in fragments shorter than     729 bp
  80% of sequence in fragments shorter than     822 bp
  85% of sequence in fragments shorter than     940 bp
  90% of sequence in fragments shorter than    1107 bp
  95% of sequence in fragments shorter than    1388 bp

# Tsp45I.TspRI (GTSAC+CASTG) double digest, 12786 thousand fragments (4021761+8765240 sites), 233 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     208 bp
  25% of sequence in fragments shorter than     237 bp
  30% of sequence in fragments shorter than     273 bp
  35% of sequence in fragments shorter than     309 bp
  40% of sequence in fragments shorter than     346 bp
  45% of sequence in fragments shorter than     385 bp
  50% of sequence in fragments shorter than     428 bp
  55% of sequence in fragments shorter than     475 bp
  60% of sequence in fragments shorter than     525 bp
  65% of sequence in fragments shorter than     582 bp
  70% of sequence in fragments shorter than     648 bp
  75% of sequence in fragments shorter than     723 bp
  80% of sequence in fragments shorter than     816 bp
  85% of sequence in fragments shorter than     936 bp
  90% of sequence in fragments shorter than    1093 bp
  95% of sequence in fragments shorter than    1379 bp

# BspHI.ScrFI (TCATGA+CCNGG) double digest, 12782 thousand fragments (978290+11805099 sites), 233 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     235 bp
  30% of sequence in fragments shorter than     286 bp
  35% of sequence in fragments shorter than     339 bp
  40% of sequence in fragments shorter than     396 bp
  45% of sequence in fragments shorter than     459 bp
  50% of sequence in fragments shorter than     528 bp
  55% of sequence in fragments shorter than     602 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     779 bp
  70% of sequence in fragments shorter than     892 bp
  75% of sequence in fragments shorter than    1025 bp
  80% of sequence in fragments shorter than    1195 bp
  85% of sequence in fragments shorter than    1418 bp
  90% of sequence in fragments shorter than    1750 bp
  95% of sequence in fragments shorter than    2373 bp

# AciI.HaeIII (CCGC+GGCC) double digest, 12767 thousand fragments (4206130+8562230 sites), 233 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     122 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     220 bp
  25% of sequence in fragments shorter than     276 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     444 bp
  45% of sequence in fragments shorter than     511 bp
  50% of sequence in fragments shorter than     582 bp
  55% of sequence in fragments shorter than     663 bp
  60% of sequence in fragments shorter than     751 bp
  65% of sequence in fragments shorter than     852 bp
  70% of sequence in fragments shorter than     965 bp
  75% of sequence in fragments shorter than    1100 bp
  80% of sequence in fragments shorter than    1268 bp
  85% of sequence in fragments shorter than    1486 bp
  90% of sequence in fragments shorter than    1798 bp
  95% of sequence in fragments shorter than    2340 bp

# AvaIII.ScrFI (ATGCAT+CCNGG) double digest, 12737 thousand fragments (933357+11805099 sites), 233 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     236 bp
  30% of sequence in fragments shorter than     286 bp
  35% of sequence in fragments shorter than     339 bp
  40% of sequence in fragments shorter than     395 bp
  45% of sequence in fragments shorter than     457 bp
  50% of sequence in fragments shorter than     524 bp
  55% of sequence in fragments shorter than     598 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     772 bp
  70% of sequence in fragments shorter than     883 bp
  75% of sequence in fragments shorter than    1017 bp
  80% of sequence in fragments shorter than    1185 bp
  85% of sequence in fragments shorter than    1408 bp
  90% of sequence in fragments shorter than    1749 bp
  95% of sequence in fragments shorter than    2349 bp

# ScrFI.StuI (CCNGG+AGGCCT) double digest, 12730 thousand fragments (11805099+925729 sites), 234 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     192 bp
  25% of sequence in fragments shorter than     242 bp
  30% of sequence in fragments shorter than     297 bp
  35% of sequence in fragments shorter than     349 bp
  40% of sequence in fragments shorter than     411 bp
  45% of sequence in fragments shorter than     481 bp
  50% of sequence in fragments shorter than     555 bp
  55% of sequence in fragments shorter than     638 bp
  60% of sequence in fragments shorter than     717 bp
  65% of sequence in fragments shorter than     822 bp
  70% of sequence in fragments shorter than     943 bp
  75% of sequence in fragments shorter than    1089 bp
  80% of sequence in fragments shorter than    1269 bp
  85% of sequence in fragments shorter than    1502 bp
  90% of sequence in fragments shorter than    1846 bp
  95% of sequence in fragments shorter than    2406 bp

# MboII.SfeI (GAAGA+CTRYAG) double digest, 12730 thousand fragments (9199496+3531009 sites), 234 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     126 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     192 bp
  25% of sequence in fragments shorter than     225 bp
  30% of sequence in fragments shorter than     259 bp
  35% of sequence in fragments shorter than     294 bp
  40% of sequence in fragments shorter than     329 bp
  45% of sequence in fragments shorter than     366 bp
  50% of sequence in fragments shorter than     408 bp
  55% of sequence in fragments shorter than     452 bp
  60% of sequence in fragments shorter than     499 bp
  65% of sequence in fragments shorter than     551 bp
  70% of sequence in fragments shorter than     611 bp
  75% of sequence in fragments shorter than     678 bp
  80% of sequence in fragments shorter than     760 bp
  85% of sequence in fragments shorter than     864 bp
  90% of sequence in fragments shorter than    1006 bp
  95% of sequence in fragments shorter than    1236 bp

# NdeI.ScrFI (CATATG+CCNGG) double digest, 12707 thousand fragments (903360+11805099 sites), 234 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     238 bp
  30% of sequence in fragments shorter than     288 bp
  35% of sequence in fragments shorter than     339 bp
  40% of sequence in fragments shorter than     395 bp
  45% of sequence in fragments shorter than     456 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     595 bp
  60% of sequence in fragments shorter than     672 bp
  65% of sequence in fragments shorter than     762 bp
  70% of sequence in fragments shorter than     866 bp
  75% of sequence in fragments shorter than     995 bp
  80% of sequence in fragments shorter than    1147 bp
  85% of sequence in fragments shorter than    1345 bp
  90% of sequence in fragments shorter than    1651 bp
  95% of sequence in fragments shorter than    2266 bp

# MlyI.TspRI (GAGTC+CASTG) double digest, 12696 thousand fragments (3931692+8765240 sites), 234 bp average fragment length
   5% of sequence in fragments shorter than      79 bp
  10% of sequence in fragments shorter than     126 bp
  15% of sequence in fragments shorter than     166 bp
  20% of sequence in fragments shorter than     203 bp
  25% of sequence in fragments shorter than     234 bp
  30% of sequence in fragments shorter than     269 bp
  35% of sequence in fragments shorter than     305 bp
  40% of sequence in fragments shorter than     342 bp
  45% of sequence in fragments shorter than     383 bp
  50% of sequence in fragments shorter than     426 bp
  55% of sequence in fragments shorter than     473 bp
  60% of sequence in fragments shorter than     525 bp
  65% of sequence in fragments shorter than     583 bp
  70% of sequence in fragments shorter than     649 bp
  75% of sequence in fragments shorter than     725 bp
  80% of sequence in fragments shorter than     820 bp
  85% of sequence in fragments shorter than     941 bp
  90% of sequence in fragments shorter than    1101 bp
  95% of sequence in fragments shorter than    1405 bp

# HindIII.ScrFI (AAGCTT+CCNGG) double digest, 12664 thousand fragments (860365+11805099 sites), 235 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     143 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     238 bp
  30% of sequence in fragments shorter than     290 bp
  35% of sequence in fragments shorter than     343 bp
  40% of sequence in fragments shorter than     401 bp
  45% of sequence in fragments shorter than     466 bp
  50% of sequence in fragments shorter than     536 bp
  55% of sequence in fragments shorter than     611 bp
  60% of sequence in fragments shorter than     695 bp
  65% of sequence in fragments shorter than     793 bp
  70% of sequence in fragments shorter than     910 bp
  75% of sequence in fragments shorter than    1048 bp
  80% of sequence in fragments shorter than    1224 bp
  85% of sequence in fragments shorter than    1451 bp
  90% of sequence in fragments shorter than    1798 bp
  95% of sequence in fragments shorter than    2410 bp

# ScrFI.XbaI (CCNGG+TCTAGA) double digest, 12655 thousand fragments (11805099+850998 sites), 235 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     191 bp
  25% of sequence in fragments shorter than     239 bp
  30% of sequence in fragments shorter than     292 bp
  35% of sequence in fragments shorter than     346 bp
  40% of sequence in fragments shorter than     402 bp
  45% of sequence in fragments shorter than     466 bp
  50% of sequence in fragments shorter than     536 bp
  55% of sequence in fragments shorter than     611 bp
  60% of sequence in fragments shorter than     693 bp
  65% of sequence in fragments shorter than     789 bp
  70% of sequence in fragments shorter than     899 bp
  75% of sequence in fragments shorter than    1032 bp
  80% of sequence in fragments shorter than    1195 bp
  85% of sequence in fragments shorter than    1391 bp
  90% of sequence in fragments shorter than    1689 bp
  95% of sequence in fragments shorter than    2230 bp

# EcoRI.ScrFI (GAATTC+CCNGG) double digest, 12652 thousand fragments (847401+11805099 sites), 235 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     192 bp
  25% of sequence in fragments shorter than     241 bp
  30% of sequence in fragments shorter than     293 bp
  35% of sequence in fragments shorter than     345 bp
  40% of sequence in fragments shorter than     402 bp
  45% of sequence in fragments shorter than     467 bp
  50% of sequence in fragments shorter than     538 bp
  55% of sequence in fragments shorter than     614 bp
  60% of sequence in fragments shorter than     694 bp
  65% of sequence in fragments shorter than     793 bp
  70% of sequence in fragments shorter than     909 bp
  75% of sequence in fragments shorter than    1045 bp
  80% of sequence in fragments shorter than    1220 bp
  85% of sequence in fragments shorter than    1442 bp
  90% of sequence in fragments shorter than    1775 bp
  95% of sequence in fragments shorter than    2341 bp

# BsmAI.HphI (GTCTC+GGTGA) double digest, 12638 thousand fragments (6631585+6007336 sites), 235 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     128 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     193 bp
  25% of sequence in fragments shorter than     232 bp
  30% of sequence in fragments shorter than     272 bp
  35% of sequence in fragments shorter than     313 bp
  40% of sequence in fragments shorter than     355 bp
  45% of sequence in fragments shorter than     398 bp
  50% of sequence in fragments shorter than     445 bp
  55% of sequence in fragments shorter than     497 bp
  60% of sequence in fragments shorter than     553 bp
  65% of sequence in fragments shorter than     615 bp
  70% of sequence in fragments shorter than     687 bp
  75% of sequence in fragments shorter than     773 bp
  80% of sequence in fragments shorter than     874 bp
  85% of sequence in fragments shorter than    1010 bp
  90% of sequence in fragments shorter than    1198 bp
  95% of sequence in fragments shorter than    1533 bp

# AvaII.BstNI (GGWCC+CCWGG) double digest, 12602 thousand fragments (2747690+9855646 sites), 236 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     115 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     191 bp
  25% of sequence in fragments shorter than     238 bp
  30% of sequence in fragments shorter than     287 bp
  35% of sequence in fragments shorter than     336 bp
  40% of sequence in fragments shorter than     388 bp
  45% of sequence in fragments shorter than     449 bp
  50% of sequence in fragments shorter than     516 bp
  55% of sequence in fragments shorter than     588 bp
  60% of sequence in fragments shorter than     668 bp
  65% of sequence in fragments shorter than     758 bp
  70% of sequence in fragments shorter than     868 bp
  75% of sequence in fragments shorter than    1003 bp
  80% of sequence in fragments shorter than    1170 bp
  85% of sequence in fragments shorter than    1390 bp
  90% of sequence in fragments shorter than    1721 bp
  95% of sequence in fragments shorter than    2306 bp

# BscAI.Hpy188I (GCATC+TCNGA) double digest, 12594 thousand fragments (3652926+8942153 sites), 236 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     122 bp
  15% of sequence in fragments shorter than     160 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     226 bp
  30% of sequence in fragments shorter than     261 bp
  35% of sequence in fragments shorter than     298 bp
  40% of sequence in fragments shorter than     336 bp
  45% of sequence in fragments shorter than     373 bp
  50% of sequence in fragments shorter than     417 bp
  55% of sequence in fragments shorter than     463 bp
  60% of sequence in fragments shorter than     514 bp
  65% of sequence in fragments shorter than     570 bp
  70% of sequence in fragments shorter than     634 bp
  75% of sequence in fragments shorter than     707 bp
  80% of sequence in fragments shorter than     796 bp
  85% of sequence in fragments shorter than     906 bp
  90% of sequence in fragments shorter than    1060 bp
  95% of sequence in fragments shorter than    1324 bp

# ApoI.BthCI (RAATTY+GCNGC) double digest, 12591 thousand fragments (6382433+6209929 sites), 236 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     163 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     238 bp
  30% of sequence in fragments shorter than     273 bp
  35% of sequence in fragments shorter than     310 bp
  40% of sequence in fragments shorter than     347 bp
  45% of sequence in fragments shorter than     388 bp
  50% of sequence in fragments shorter than     430 bp
  55% of sequence in fragments shorter than     477 bp
  60% of sequence in fragments shorter than     528 bp
  65% of sequence in fragments shorter than     583 bp
  70% of sequence in fragments shorter than     647 bp
  75% of sequence in fragments shorter than     719 bp
  80% of sequence in fragments shorter than     808 bp
  85% of sequence in fragments shorter than     914 bp
  90% of sequence in fragments shorter than    1066 bp
  95% of sequence in fragments shorter than    1314 bp

# HaeIII.Tsp45I (GGCC+GTSAC) double digest, 12583 thousand fragments (8562230+4021761 sites), 236 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     197 bp
  25% of sequence in fragments shorter than     236 bp
  30% of sequence in fragments shorter than     276 bp
  35% of sequence in fragments shorter than     320 bp
  40% of sequence in fragments shorter than     360 bp
  45% of sequence in fragments shorter than     409 bp
  50% of sequence in fragments shorter than     459 bp
  55% of sequence in fragments shorter than     516 bp
  60% of sequence in fragments shorter than     579 bp
  65% of sequence in fragments shorter than     648 bp
  70% of sequence in fragments shorter than     730 bp
  75% of sequence in fragments shorter than     828 bp
  80% of sequence in fragments shorter than     941 bp
  85% of sequence in fragments shorter than    1093 bp
  90% of sequence in fragments shorter than    1296 bp
  95% of sequence in fragments shorter than    1658 bp

# BstNI.SmlI (CCWGG+CTYRAG) double digest, 12583 thousand fragments (9855646+2727867 sites), 236 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     114 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     186 bp
  25% of sequence in fragments shorter than     230 bp
  30% of sequence in fragments shorter than     275 bp
  35% of sequence in fragments shorter than     322 bp
  40% of sequence in fragments shorter than     371 bp
  45% of sequence in fragments shorter than     426 bp
  50% of sequence in fragments shorter than     485 bp
  55% of sequence in fragments shorter than     551 bp
  60% of sequence in fragments shorter than     622 bp
  65% of sequence in fragments shorter than     702 bp
  70% of sequence in fragments shorter than     798 bp
  75% of sequence in fragments shorter than     914 bp
  80% of sequence in fragments shorter than    1059 bp
  85% of sequence in fragments shorter than    1252 bp
  90% of sequence in fragments shorter than    1538 bp
  95% of sequence in fragments shorter than    2089 bp

# BglII.ScrFI (AGATCT+CCNGG) double digest, 12579 thousand fragments (774732+11805099 sites), 236 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     191 bp
  25% of sequence in fragments shorter than     240 bp
  30% of sequence in fragments shorter than     293 bp
  35% of sequence in fragments shorter than     346 bp
  40% of sequence in fragments shorter than     405 bp
  45% of sequence in fragments shorter than     470 bp
  50% of sequence in fragments shorter than     542 bp
  55% of sequence in fragments shorter than     620 bp
  60% of sequence in fragments shorter than     708 bp
  65% of sequence in fragments shorter than     808 bp
  70% of sequence in fragments shorter than     928 bp
  75% of sequence in fragments shorter than    1073 bp
  80% of sequence in fragments shorter than    1255 bp
  85% of sequence in fragments shorter than    1501 bp
  90% of sequence in fragments shorter than    1869 bp
  95% of sequence in fragments shorter than    2582 bp

# BbvI.BspNCI (GCAGC+CCAGA) double digest, 12571 thousand fragments (5290051+7282436 sites), 237 bp average fragment length
   5% of sequence in fragments shorter than      77 bp
  10% of sequence in fragments shorter than     121 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     241 bp
  30% of sequence in fragments shorter than     281 bp
  35% of sequence in fragments shorter than     323 bp
  40% of sequence in fragments shorter than     366 bp
  45% of sequence in fragments shorter than     413 bp
  50% of sequence in fragments shorter than     462 bp
  55% of sequence in fragments shorter than     516 bp
  60% of sequence in fragments shorter than     575 bp
  65% of sequence in fragments shorter than     639 bp
  70% of sequence in fragments shorter than     714 bp
  75% of sequence in fragments shorter than     803 bp
  80% of sequence in fragments shorter than     911 bp
  85% of sequence in fragments shorter than    1050 bp
  90% of sequence in fragments shorter than    1232 bp
  95% of sequence in fragments shorter than    1563 bp

# BscAI.HinfI (GCATC+GANTC) double digest, 12568 thousand fragments (3652926+8916054 sites), 237 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     121 bp
  15% of sequence in fragments shorter than     159 bp
  20% of sequence in fragments shorter than     192 bp
  25% of sequence in fragments shorter than     227 bp
  30% of sequence in fragments shorter than     260 bp
  35% of sequence in fragments shorter than     294 bp
  40% of sequence in fragments shorter than     328 bp
  45% of sequence in fragments shorter than     358 bp
  50% of sequence in fragments shorter than     395 bp
  55% of sequence in fragments shorter than     438 bp
  60% of sequence in fragments shorter than     483 bp
  65% of sequence in fragments shorter than     533 bp
  70% of sequence in fragments shorter than     590 bp
  75% of sequence in fragments shorter than     657 bp
  80% of sequence in fragments shorter than     734 bp
  85% of sequence in fragments shorter than     835 bp
  90% of sequence in fragments shorter than     973 bp
  95% of sequence in fragments shorter than    1205 bp

# NcoI.ScrFI (CCATGG+CCNGG) double digest, 12564 thousand fragments (759594+11805099 sites), 237 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     105 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     192 bp
  25% of sequence in fragments shorter than     242 bp
  30% of sequence in fragments shorter than     296 bp
  35% of sequence in fragments shorter than     352 bp
  40% of sequence in fragments shorter than     414 bp
  45% of sequence in fragments shorter than     483 bp
  50% of sequence in fragments shorter than     558 bp
  55% of sequence in fragments shorter than     640 bp
  60% of sequence in fragments shorter than     731 bp
  65% of sequence in fragments shorter than     838 bp
  70% of sequence in fragments shorter than     965 bp
  75% of sequence in fragments shorter than    1116 bp
  80% of sequence in fragments shorter than    1308 bp
  85% of sequence in fragments shorter than    1557 bp
  90% of sequence in fragments shorter than    1896 bp
  95% of sequence in fragments shorter than    2611 bp

# ApoI.BccI (RAATTY+CCATC) double digest, 12552 thousand fragments (6382433+6170927 sites), 237 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     194 bp
  25% of sequence in fragments shorter than     227 bp
  30% of sequence in fragments shorter than     259 bp
  35% of sequence in fragments shorter than     293 bp
  40% of sequence in fragments shorter than     327 bp
  45% of sequence in fragments shorter than     361 bp
  50% of sequence in fragments shorter than     400 bp
  55% of sequence in fragments shorter than     441 bp
  60% of sequence in fragments shorter than     486 bp
  65% of sequence in fragments shorter than     537 bp
  70% of sequence in fragments shorter than     594 bp
  75% of sequence in fragments shorter than     658 bp
  80% of sequence in fragments shorter than     734 bp
  85% of sequence in fragments shorter than     832 bp
  90% of sequence in fragments shorter than     973 bp
  95% of sequence in fragments shorter than    1218 bp

# BclI.ScrFI (TGATCA+CCNGG) double digest, 12543 thousand fragments (738785+11805099 sites), 237 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     193 bp
  25% of sequence in fragments shorter than     242 bp
  30% of sequence in fragments shorter than     295 bp
  35% of sequence in fragments shorter than     350 bp
  40% of sequence in fragments shorter than     410 bp
  45% of sequence in fragments shorter than     476 bp
  50% of sequence in fragments shorter than     548 bp
  55% of sequence in fragments shorter than     626 bp
  60% of sequence in fragments shorter than     713 bp
  65% of sequence in fragments shorter than     815 bp
  70% of sequence in fragments shorter than     934 bp
  75% of sequence in fragments shorter than    1077 bp
  80% of sequence in fragments shorter than    1259 bp
  85% of sequence in fragments shorter than    1500 bp
  90% of sequence in fragments shorter than    1863 bp
  95% of sequence in fragments shorter than    2554 bp

# FnuDII.ScrFI (CGCG+CCNGG) double digest, 12538 thousand fragments (733940+11805099 sites), 237 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     199 bp
  25% of sequence in fragments shorter than     254 bp
  30% of sequence in fragments shorter than     313 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     444 bp
  45% of sequence in fragments shorter than     519 bp
  50% of sequence in fragments shorter than     601 bp
  55% of sequence in fragments shorter than     691 bp
  60% of sequence in fragments shorter than     793 bp
  65% of sequence in fragments shorter than     914 bp
  70% of sequence in fragments shorter than    1055 bp
  75% of sequence in fragments shorter than    1228 bp
  80% of sequence in fragments shorter than    1443 bp
  85% of sequence in fragments shorter than    1736 bp
  90% of sequence in fragments shorter than    2152 bp
  95% of sequence in fragments shorter than    2957 bp

# BsaAI.ScrFI (YACGTR+CCNGG) double digest, 12518 thousand fragments (713801+11805099 sites), 238 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     193 bp
  25% of sequence in fragments shorter than     243 bp
  30% of sequence in fragments shorter than     296 bp
  35% of sequence in fragments shorter than     352 bp
  40% of sequence in fragments shorter than     413 bp
  45% of sequence in fragments shorter than     481 bp
  50% of sequence in fragments shorter than     555 bp
  55% of sequence in fragments shorter than     636 bp
  60% of sequence in fragments shorter than     725 bp
  65% of sequence in fragments shorter than     830 bp
  70% of sequence in fragments shorter than     954 bp
  75% of sequence in fragments shorter than    1098 bp
  80% of sequence in fragments shorter than    1282 bp
  85% of sequence in fragments shorter than    1522 bp
  90% of sequence in fragments shorter than    1858 bp
  95% of sequence in fragments shorter than    2473 bp

# BceAI.ScrFI (ACGGC+CCNGG) double digest, 12509 thousand fragments (705178+11805099 sites), 238 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     106 bp
  15% of sequence in fragments shorter than     145 bp
  20% of sequence in fragments shorter than     194 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     302 bp
  35% of sequence in fragments shorter than     361 bp
  40% of sequence in fragments shorter than     426 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     577 bp
  55% of sequence in fragments shorter than     661 bp
  60% of sequence in fragments shorter than     758 bp
  65% of sequence in fragments shorter than     872 bp
  70% of sequence in fragments shorter than    1007 bp
  75% of sequence in fragments shorter than    1170 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1649 bp
  90% of sequence in fragments shorter than    2048 bp
  95% of sequence in fragments shorter than    2810 bp

# BciVI.ScrFI (GTATCC+CCNGG) double digest, 12500 thousand fragments (696093+11805099 sites), 238 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     146 bp
  20% of sequence in fragments shorter than     193 bp
  25% of sequence in fragments shorter than     243 bp
  30% of sequence in fragments shorter than     296 bp
  35% of sequence in fragments shorter than     352 bp
  40% of sequence in fragments shorter than     412 bp
  45% of sequence in fragments shorter than     479 bp
  50% of sequence in fragments shorter than     552 bp
  55% of sequence in fragments shorter than     631 bp
  60% of sequence in fragments shorter than     719 bp
  65% of sequence in fragments shorter than     821 bp
  70% of sequence in fragments shorter than     944 bp
  75% of sequence in fragments shorter than    1088 bp
  80% of sequence in fragments shorter than    1271 bp
  85% of sequence in fragments shorter than    1515 bp
  90% of sequence in fragments shorter than    1865 bp
  95% of sequence in fragments shorter than    2538 bp

# HaeIII.MlyI (GGCC+GAGTC) double digest, 12493 thousand fragments (8562230+3931692 sites), 238 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     195 bp
  25% of sequence in fragments shorter than     232 bp
  30% of sequence in fragments shorter than     275 bp
  35% of sequence in fragments shorter than     320 bp
  40% of sequence in fragments shorter than     360 bp
  45% of sequence in fragments shorter than     412 bp
  50% of sequence in fragments shorter than     468 bp
  55% of sequence in fragments shorter than     526 bp
  60% of sequence in fragments shorter than     594 bp
  65% of sequence in fragments shorter than     671 bp
  70% of sequence in fragments shorter than     757 bp
  75% of sequence in fragments shorter than     860 bp
  80% of sequence in fragments shorter than     980 bp
  85% of sequence in fragments shorter than    1133 bp
  90% of sequence in fragments shorter than    1354 bp
  95% of sequence in fragments shorter than    1736 bp

# BbvI.BspKT6I (GCAGC+GATC) double digest, 12488 thousand fragments (5290051+7199434 sites), 238 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     227 bp
  30% of sequence in fragments shorter than     263 bp
  35% of sequence in fragments shorter than     300 bp
  40% of sequence in fragments shorter than     338 bp
  45% of sequence in fragments shorter than     378 bp
  50% of sequence in fragments shorter than     421 bp
  55% of sequence in fragments shorter than     467 bp
  60% of sequence in fragments shorter than     517 bp
  65% of sequence in fragments shorter than     573 bp
  70% of sequence in fragments shorter than     638 bp
  75% of sequence in fragments shorter than     714 bp
  80% of sequence in fragments shorter than     809 bp
  85% of sequence in fragments shorter than     930 bp
  90% of sequence in fragments shorter than    1086 bp
  95% of sequence in fragments shorter than    1380 bp

# BspNCI.FokI (CCAGA+GGATG) double digest, 12483 thousand fragments (7282436+5201116 sites), 238 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     198 bp
  25% of sequence in fragments shorter than     236 bp
  30% of sequence in fragments shorter than     271 bp
  35% of sequence in fragments shorter than     308 bp
  40% of sequence in fragments shorter than     347 bp
  45% of sequence in fragments shorter than     389 bp
  50% of sequence in fragments shorter than     433 bp
  55% of sequence in fragments shorter than     482 bp
  60% of sequence in fragments shorter than     534 bp
  65% of sequence in fragments shorter than     592 bp
  70% of sequence in fragments shorter than     658 bp
  75% of sequence in fragments shorter than     732 bp
  80% of sequence in fragments shorter than     826 bp
  85% of sequence in fragments shorter than     944 bp
  90% of sequence in fragments shorter than    1114 bp
  95% of sequence in fragments shorter than    1411 bp

# Hpy188I.SfeI (TCNGA+CTRYAG) double digest, 12472 thousand fragments (8942153+3531009 sites), 238 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     123 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     190 bp
  25% of sequence in fragments shorter than     224 bp
  30% of sequence in fragments shorter than     258 bp
  35% of sequence in fragments shorter than     293 bp
  40% of sequence in fragments shorter than     328 bp
  45% of sequence in fragments shorter than     362 bp
  50% of sequence in fragments shorter than     403 bp
  55% of sequence in fragments shorter than     446 bp
  60% of sequence in fragments shorter than     493 bp
  65% of sequence in fragments shorter than     545 bp
  70% of sequence in fragments shorter than     602 bp
  75% of sequence in fragments shorter than     671 bp
  80% of sequence in fragments shorter than     754 bp
  85% of sequence in fragments shorter than     856 bp
  90% of sequence in fragments shorter than     999 bp
  95% of sequence in fragments shorter than    1221 bp

# AflII.ScrFI (CTTAAG+CCNGG) double digest, 12447 thousand fragments (643284+11805099 sites), 239 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     146 bp
  20% of sequence in fragments shorter than     194 bp
  25% of sequence in fragments shorter than     244 bp
  30% of sequence in fragments shorter than     297 bp
  35% of sequence in fragments shorter than     353 bp
  40% of sequence in fragments shorter than     413 bp
  45% of sequence in fragments shorter than     479 bp
  50% of sequence in fragments shorter than     553 bp
  55% of sequence in fragments shorter than     632 bp
  60% of sequence in fragments shorter than     720 bp
  65% of sequence in fragments shorter than     822 bp
  70% of sequence in fragments shorter than     945 bp
  75% of sequence in fragments shorter than    1092 bp
  80% of sequence in fragments shorter than    1276 bp
  85% of sequence in fragments shorter than    1525 bp
  90% of sequence in fragments shorter than    1890 bp
  95% of sequence in fragments shorter than    2606 bp

# HinfI.SfeI (GANTC+CTRYAG) double digest, 12446 thousand fragments (8916054+3531009 sites), 239 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     122 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     195 bp
  25% of sequence in fragments shorter than     230 bp
  30% of sequence in fragments shorter than     264 bp
  35% of sequence in fragments shorter than     299 bp
  40% of sequence in fragments shorter than     332 bp
  45% of sequence in fragments shorter than     364 bp
  50% of sequence in fragments shorter than     403 bp
  55% of sequence in fragments shorter than     446 bp
  60% of sequence in fragments shorter than     492 bp
  65% of sequence in fragments shorter than     542 bp
  70% of sequence in fragments shorter than     601 bp
  75% of sequence in fragments shorter than     666 bp
  80% of sequence in fragments shorter than     743 bp
  85% of sequence in fragments shorter than     842 bp
  90% of sequence in fragments shorter than     981 bp
  95% of sequence in fragments shorter than    1214 bp

# HaeII.ScrFI (RGCGCY+CCNGG) double digest, 12434 thousand fragments (630101+11805099 sites), 239 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     200 bp
  25% of sequence in fragments shorter than     254 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     369 bp
  40% of sequence in fragments shorter than     436 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     676 bp
  60% of sequence in fragments shorter than     767 bp
  65% of sequence in fragments shorter than     881 bp
  70% of sequence in fragments shorter than    1017 bp
  75% of sequence in fragments shorter than    1173 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1630 bp
  90% of sequence in fragments shorter than    1998 bp
  95% of sequence in fragments shorter than    2607 bp

# BscAI.TspRI (GCATC+CASTG) double digest, 12417 thousand fragments (3652926+8765240 sites), 239 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     168 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     234 bp
  30% of sequence in fragments shorter than     268 bp
  35% of sequence in fragments shorter than     303 bp
  40% of sequence in fragments shorter than     340 bp
  45% of sequence in fragments shorter than     378 bp
  50% of sequence in fragments shorter than     419 bp
  55% of sequence in fragments shorter than     464 bp
  60% of sequence in fragments shorter than     513 bp
  65% of sequence in fragments shorter than     568 bp
  70% of sequence in fragments shorter than     631 bp
  75% of sequence in fragments shorter than     697 bp
  80% of sequence in fragments shorter than     783 bp
  85% of sequence in fragments shorter than     895 bp
  90% of sequence in fragments shorter than    1056 bp
  95% of sequence in fragments shorter than    1349 bp

# SacI.ScrFI (GAGCTC+CCNGG) double digest, 12407 thousand fragments (603323+11805099 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     146 bp
  20% of sequence in fragments shorter than     196 bp
  25% of sequence in fragments shorter than     247 bp
  30% of sequence in fragments shorter than     304 bp
  35% of sequence in fragments shorter than     363 bp
  40% of sequence in fragments shorter than     427 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     577 bp
  55% of sequence in fragments shorter than     660 bp
  60% of sequence in fragments shorter than     757 bp
  65% of sequence in fragments shorter than     871 bp
  70% of sequence in fragments shorter than    1004 bp
  75% of sequence in fragments shorter than    1166 bp
  80% of sequence in fragments shorter than    1368 bp
  85% of sequence in fragments shorter than    1645 bp
  90% of sequence in fragments shorter than    2049 bp
  95% of sequence in fragments shorter than    2798 bp

# BspKT6I.FokI (GATC+GGATG) double digest, 12400 thousand fragments (7199434+5201116 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      88 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     164 bp
  20% of sequence in fragments shorter than     191 bp
  25% of sequence in fragments shorter than     227 bp
  30% of sequence in fragments shorter than     263 bp
  35% of sequence in fragments shorter than     298 bp
  40% of sequence in fragments shorter than     334 bp
  45% of sequence in fragments shorter than     369 bp
  50% of sequence in fragments shorter than     410 bp
  55% of sequence in fragments shorter than     452 bp
  60% of sequence in fragments shorter than     498 bp
  65% of sequence in fragments shorter than     551 bp
  70% of sequence in fragments shorter than     611 bp
  75% of sequence in fragments shorter than     681 bp
  80% of sequence in fragments shorter than     766 bp
  85% of sequence in fragments shorter than     874 bp
  90% of sequence in fragments shorter than    1028 bp
  95% of sequence in fragments shorter than    1293 bp

# AvrII.ScrFI (CCTAGG+CCNGG) double digest, 12399 thousand fragments (594956+11805099 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     146 bp
  20% of sequence in fragments shorter than     196 bp
  25% of sequence in fragments shorter than     247 bp
  30% of sequence in fragments shorter than     302 bp
  35% of sequence in fragments shorter than     360 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     494 bp
  50% of sequence in fragments shorter than     572 bp
  55% of sequence in fragments shorter than     651 bp
  60% of sequence in fragments shorter than     743 bp
  65% of sequence in fragments shorter than     853 bp
  70% of sequence in fragments shorter than     977 bp
  75% of sequence in fragments shorter than    1131 bp
  80% of sequence in fragments shorter than    1320 bp
  85% of sequence in fragments shorter than    1575 bp
  90% of sequence in fragments shorter than    1952 bp
  95% of sequence in fragments shorter than    2721 bp

# BspNCI.CviQI (CCAGA+GTAC) double digest, 12397 thousand fragments (7282436+5115078 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     198 bp
  25% of sequence in fragments shorter than     233 bp
  30% of sequence in fragments shorter than     268 bp
  35% of sequence in fragments shorter than     304 bp
  40% of sequence in fragments shorter than     341 bp
  45% of sequence in fragments shorter than     379 bp
  50% of sequence in fragments shorter than     421 bp
  55% of sequence in fragments shorter than     464 bp
  60% of sequence in fragments shorter than     509 bp
  65% of sequence in fragments shorter than     561 bp
  70% of sequence in fragments shorter than     617 bp
  75% of sequence in fragments shorter than     685 bp
  80% of sequence in fragments shorter than     765 bp
  85% of sequence in fragments shorter than     866 bp
  90% of sequence in fragments shorter than    1011 bp
  95% of sequence in fragments shorter than    1258 bp

# ApoI.HphI (RAATTY+GGTGA) double digest, 12389 thousand fragments (6382433+6007336 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     127 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     192 bp
  25% of sequence in fragments shorter than     226 bp
  30% of sequence in fragments shorter than     258 bp
  35% of sequence in fragments shorter than     292 bp
  40% of sequence in fragments shorter than     327 bp
  45% of sequence in fragments shorter than     361 bp
  50% of sequence in fragments shorter than     400 bp
  55% of sequence in fragments shorter than     442 bp
  60% of sequence in fragments shorter than     487 bp
  65% of sequence in fragments shorter than     537 bp
  70% of sequence in fragments shorter than     592 bp
  75% of sequence in fragments shorter than     657 bp
  80% of sequence in fragments shorter than     734 bp
  85% of sequence in fragments shorter than     836 bp
  90% of sequence in fragments shorter than     967 bp
  95% of sequence in fragments shorter than    1187 bp

# BccI.BthCI (CCATC+GCNGC) double digest, 12380 thousand fragments (6170927+6209929 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      79 bp
  10% of sequence in fragments shorter than     127 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     208 bp
  25% of sequence in fragments shorter than     249 bp
  30% of sequence in fragments shorter than     290 bp
  35% of sequence in fragments shorter than     335 bp
  40% of sequence in fragments shorter than     379 bp
  45% of sequence in fragments shorter than     428 bp
  50% of sequence in fragments shorter than     481 bp
  55% of sequence in fragments shorter than     539 bp
  60% of sequence in fragments shorter than     603 bp
  65% of sequence in fragments shorter than     673 bp
  70% of sequence in fragments shorter than     755 bp
  75% of sequence in fragments shorter than     852 bp
  80% of sequence in fragments shorter than     971 bp
  85% of sequence in fragments shorter than    1114 bp
  90% of sequence in fragments shorter than    1325 bp
  95% of sequence in fragments shorter than    1698 bp

# HgaI.ScrFI (GACGC+CCNGG) double digest, 12376 thousand fragments (571889+11805099 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     197 bp
  25% of sequence in fragments shorter than     250 bp
  30% of sequence in fragments shorter than     307 bp
  35% of sequence in fragments shorter than     368 bp
  40% of sequence in fragments shorter than     434 bp
  45% of sequence in fragments shorter than     507 bp
  50% of sequence in fragments shorter than     588 bp
  55% of sequence in fragments shorter than     674 bp
  60% of sequence in fragments shorter than     773 bp
  65% of sequence in fragments shorter than     891 bp
  70% of sequence in fragments shorter than    1027 bp
  75% of sequence in fragments shorter than    1194 bp
  80% of sequence in fragments shorter than    1399 bp
  85% of sequence in fragments shorter than    1682 bp
  90% of sequence in fragments shorter than    2080 bp
  95% of sequence in fragments shorter than    2821 bp

# EciI.ScrFI (GGCGGA+CCNGG) double digest, 12375 thousand fragments (571273+11805099 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     149 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     256 bp
  30% of sequence in fragments shorter than     316 bp
  35% of sequence in fragments shorter than     378 bp
  40% of sequence in fragments shorter than     446 bp
  45% of sequence in fragments shorter than     522 bp
  50% of sequence in fragments shorter than     603 bp
  55% of sequence in fragments shorter than     693 bp
  60% of sequence in fragments shorter than     797 bp
  65% of sequence in fragments shorter than     918 bp
  70% of sequence in fragments shorter than    1058 bp
  75% of sequence in fragments shorter than    1232 bp
  80% of sequence in fragments shorter than    1446 bp
  85% of sequence in fragments shorter than    1737 bp
  90% of sequence in fragments shorter than    2151 bp
  95% of sequence in fragments shorter than    2970 bp

# BsmAI.BsrI (GTCTC+ACTGG) double digest, 12372 thousand fragments (6631585+5741310 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     164 bp
  20% of sequence in fragments shorter than     194 bp
  25% of sequence in fragments shorter than     229 bp
  30% of sequence in fragments shorter than     268 bp
  35% of sequence in fragments shorter than     306 bp
  40% of sequence in fragments shorter than     345 bp
  45% of sequence in fragments shorter than     387 bp
  50% of sequence in fragments shorter than     432 bp
  55% of sequence in fragments shorter than     478 bp
  60% of sequence in fragments shorter than     528 bp
  65% of sequence in fragments shorter than     586 bp
  70% of sequence in fragments shorter than     652 bp
  75% of sequence in fragments shorter than     728 bp
  80% of sequence in fragments shorter than     825 bp
  85% of sequence in fragments shorter than     948 bp
  90% of sequence in fragments shorter than    1126 bp
  95% of sequence in fragments shorter than    1444 bp

# MfeI.ScrFI (CAATTG+CCNGG) double digest, 12368 thousand fragments (564303+11805099 sites), 240 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     196 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     299 bp
  35% of sequence in fragments shorter than     356 bp
  40% of sequence in fragments shorter than     416 bp
  45% of sequence in fragments shorter than     483 bp
  50% of sequence in fragments shorter than     556 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     721 bp
  65% of sequence in fragments shorter than     823 bp
  70% of sequence in fragments shorter than     943 bp
  75% of sequence in fragments shorter than    1082 bp
  80% of sequence in fragments shorter than    1261 bp
  85% of sequence in fragments shorter than    1499 bp
  90% of sequence in fragments shorter than    1846 bp
  95% of sequence in fragments shorter than    2479 bp

# ScrFI.SphI (CCNGG+GCATGC) double digest, 12355 thousand fragments (11805099+550952 sites), 241 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     107 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     197 bp
  25% of sequence in fragments shorter than     249 bp
  30% of sequence in fragments shorter than     306 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     429 bp
  45% of sequence in fragments shorter than     500 bp
  50% of sequence in fragments shorter than     579 bp
  55% of sequence in fragments shorter than     661 bp
  60% of sequence in fragments shorter than     758 bp
  65% of sequence in fragments shorter than     871 bp
  70% of sequence in fragments shorter than    1004 bp
  75% of sequence in fragments shorter than    1164 bp
  80% of sequence in fragments shorter than    1366 bp
  85% of sequence in fragments shorter than    1640 bp
  90% of sequence in fragments shorter than    2037 bp
  95% of sequence in fragments shorter than    2791 bp

# BbvI.Hin4II (GCAGC+CCTTC) double digest, 12349 thousand fragments (5290051+7059915 sites), 241 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     126 bp
  15% of sequence in fragments shorter than     168 bp
  20% of sequence in fragments shorter than     210 bp
  25% of sequence in fragments shorter than     251 bp
  30% of sequence in fragments shorter than     293 bp
  35% of sequence in fragments shorter than     337 bp
  40% of sequence in fragments shorter than     380 bp
  45% of sequence in fragments shorter than     428 bp
  50% of sequence in fragments shorter than     482 bp
  55% of sequence in fragments shorter than     535 bp
  60% of sequence in fragments shorter than     598 bp
  65% of sequence in fragments shorter than     670 bp
  70% of sequence in fragments shorter than     749 bp
  75% of sequence in fragments shorter than     844 bp
  80% of sequence in fragments shorter than     958 bp
  85% of sequence in fragments shorter than    1106 bp
  90% of sequence in fragments shorter than    1312 bp
  95% of sequence in fragments shorter than    1662 bp

# ScaI.ScrFI (AGTACT+CCNGG) double digest, 12348 thousand fragments (543793+11805099 sites), 241 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     196 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     301 bp
  35% of sequence in fragments shorter than     357 bp
  40% of sequence in fragments shorter than     419 bp
  45% of sequence in fragments shorter than     486 bp
  50% of sequence in fragments shorter than     560 bp
  55% of sequence in fragments shorter than     640 bp
  60% of sequence in fragments shorter than     729 bp
  65% of sequence in fragments shorter than     833 bp
  70% of sequence in fragments shorter than     957 bp
  75% of sequence in fragments shorter than    1105 bp
  80% of sequence in fragments shorter than    1291 bp
  85% of sequence in fragments shorter than    1546 bp
  90% of sequence in fragments shorter than    1910 bp
  95% of sequence in fragments shorter than    2620 bp

# BspKT6I.CviQI (GATC+GTAC) double digest, 12314 thousand fragments (7199434+5115078 sites), 242 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     222 bp
  30% of sequence in fragments shorter than     257 bp
  35% of sequence in fragments shorter than     291 bp
  40% of sequence in fragments shorter than     325 bp
  45% of sequence in fragments shorter than     360 bp
  50% of sequence in fragments shorter than     399 bp
  55% of sequence in fragments shorter than     441 bp
  60% of sequence in fragments shorter than     484 bp
  65% of sequence in fragments shorter than     532 bp
  70% of sequence in fragments shorter than     588 bp
  75% of sequence in fragments shorter than     655 bp
  80% of sequence in fragments shorter than     734 bp
  85% of sequence in fragments shorter than     838 bp
  90% of sequence in fragments shorter than     981 bp
  95% of sequence in fragments shorter than    1221 bp

# MboII.StyI (GAAGA+CCWWGG) double digest, 12313 thousand fragments (9199496+3114107 sites), 242 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     167 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     237 bp
  30% of sequence in fragments shorter than     274 bp
  35% of sequence in fragments shorter than     311 bp
  40% of sequence in fragments shorter than     347 bp
  45% of sequence in fragments shorter than     388 bp
  50% of sequence in fragments shorter than     431 bp
  55% of sequence in fragments shorter than     475 bp
  60% of sequence in fragments shorter than     524 bp
  65% of sequence in fragments shorter than     580 bp
  70% of sequence in fragments shorter than     642 bp
  75% of sequence in fragments shorter than     715 bp
  80% of sequence in fragments shorter than     803 bp
  85% of sequence in fragments shorter than     912 bp
  90% of sequence in fragments shorter than    1059 bp
  95% of sequence in fragments shorter than    1316 bp

# MboII.NspI (GAAGA+RCATGY) double digest, 12303 thousand fragments (9199496+3104359 sites), 242 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     168 bp
  20% of sequence in fragments shorter than     200 bp
  25% of sequence in fragments shorter than     235 bp
  30% of sequence in fragments shorter than     271 bp
  35% of sequence in fragments shorter than     309 bp
  40% of sequence in fragments shorter than     343 bp
  45% of sequence in fragments shorter than     383 bp
  50% of sequence in fragments shorter than     426 bp
  55% of sequence in fragments shorter than     471 bp
  60% of sequence in fragments shorter than     520 bp
  65% of sequence in fragments shorter than     574 bp
  70% of sequence in fragments shorter than     636 bp
  75% of sequence in fragments shorter than     709 bp
  80% of sequence in fragments shorter than     796 bp
  85% of sequence in fragments shorter than     906 bp
  90% of sequence in fragments shorter than    1053 bp
  95% of sequence in fragments shorter than    1313 bp

# ApaLI.ScrFI (GTGCAC+CCNGG) double digest, 12300 thousand fragments (496255+11805099 sites), 242 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     108 bp
  15% of sequence in fragments shorter than     147 bp
  20% of sequence in fragments shorter than     198 bp
  25% of sequence in fragments shorter than     250 bp
  30% of sequence in fragments shorter than     307 bp
  35% of sequence in fragments shorter than     366 bp
  40% of sequence in fragments shorter than     431 bp
  45% of sequence in fragments shorter than     503 bp
  50% of sequence in fragments shorter than     582 bp
  55% of sequence in fragments shorter than     666 bp
  60% of sequence in fragments shorter than     762 bp
  65% of sequence in fragments shorter than     876 bp
  70% of sequence in fragments shorter than    1009 bp
  75% of sequence in fragments shorter than    1169 bp
  80% of sequence in fragments shorter than    1369 bp
  85% of sequence in fragments shorter than    1640 bp
  90% of sequence in fragments shorter than    2004 bp
  95% of sequence in fragments shorter than    2746 bp

# SfeI.TspRI (CTRYAG+CASTG) double digest, 12295 thousand fragments (3531009+8765240 sites), 242 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     209 bp
  25% of sequence in fragments shorter than     242 bp
  30% of sequence in fragments shorter than     280 bp
  35% of sequence in fragments shorter than     318 bp
  40% of sequence in fragments shorter than     356 bp
  45% of sequence in fragments shorter than     398 bp
  50% of sequence in fragments shorter than     442 bp
  55% of sequence in fragments shorter than     488 bp
  60% of sequence in fragments shorter than     540 bp
  65% of sequence in fragments shorter than     599 bp
  70% of sequence in fragments shorter than     663 bp
  75% of sequence in fragments shorter than     739 bp
  80% of sequence in fragments shorter than     832 bp
  85% of sequence in fragments shorter than     955 bp
  90% of sequence in fragments shorter than    1126 bp
  95% of sequence in fragments shorter than    1408 bp

# BspNCI.TfiI (CCAGA+GAWTC) double digest, 12266 thousand fragments (7282436+4984362 sites), 242 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     240 bp
  30% of sequence in fragments shorter than     277 bp
  35% of sequence in fragments shorter than     313 bp
  40% of sequence in fragments shorter than     346 bp
  45% of sequence in fragments shorter than     382 bp
  50% of sequence in fragments shorter than     424 bp
  55% of sequence in fragments shorter than     469 bp
  60% of sequence in fragments shorter than     518 bp
  65% of sequence in fragments shorter than     572 bp
  70% of sequence in fragments shorter than     633 bp
  75% of sequence in fragments shorter than     702 bp
  80% of sequence in fragments shorter than     788 bp
  85% of sequence in fragments shorter than     896 bp
  90% of sequence in fragments shorter than    1044 bp
  95% of sequence in fragments shorter than    1297 bp

# ApaI.ScrFI (GGGCCC+CCNGG) double digest, 12264 thousand fragments (460339+11805099 sites), 242 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     109 bp
  15% of sequence in fragments shorter than     149 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     254 bp
  30% of sequence in fragments shorter than     313 bp
  35% of sequence in fragments shorter than     375 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     517 bp
  50% of sequence in fragments shorter than     598 bp
  55% of sequence in fragments shorter than     687 bp
  60% of sequence in fragments shorter than     789 bp
  65% of sequence in fragments shorter than     909 bp
  70% of sequence in fragments shorter than    1048 bp
  75% of sequence in fragments shorter than    1220 bp
  80% of sequence in fragments shorter than    1434 bp
  85% of sequence in fragments shorter than    1726 bp
  90% of sequence in fragments shorter than    2143 bp
  95% of sequence in fragments shorter than    2950 bp

# FokI.Hin4II (GGATG+CCTTC) double digest, 12260 thousand fragments (5201116+7059915 sites), 243 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     210 bp
  25% of sequence in fragments shorter than     249 bp
  30% of sequence in fragments shorter than     288 bp
  35% of sequence in fragments shorter than     328 bp
  40% of sequence in fragments shorter than     367 bp
  45% of sequence in fragments shorter than     412 bp
  50% of sequence in fragments shorter than     459 bp
  55% of sequence in fragments shorter than     512 bp
  60% of sequence in fragments shorter than     565 bp
  65% of sequence in fragments shorter than     628 bp
  70% of sequence in fragments shorter than     700 bp
  75% of sequence in fragments shorter than     783 bp
  80% of sequence in fragments shorter than     882 bp
  85% of sequence in fragments shorter than    1009 bp
  90% of sequence in fragments shorter than    1187 bp
  95% of sequence in fragments shorter than    1489 bp

# EcoRV.ScrFI (GATATC+CCNGG) double digest, 12251 thousand fragments (446473+11805099 sites), 243 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     148 bp
  20% of sequence in fragments shorter than     198 bp
  25% of sequence in fragments shorter than     249 bp
  30% of sequence in fragments shorter than     305 bp
  35% of sequence in fragments shorter than     363 bp
  40% of sequence in fragments shorter than     425 bp
  45% of sequence in fragments shorter than     494 bp
  50% of sequence in fragments shorter than     569 bp
  55% of sequence in fragments shorter than     650 bp
  60% of sequence in fragments shorter than     740 bp
  65% of sequence in fragments shorter than     847 bp
  70% of sequence in fragments shorter than     972 bp
  75% of sequence in fragments shorter than    1119 bp
  80% of sequence in fragments shorter than    1307 bp
  85% of sequence in fragments shorter than    1555 bp
  90% of sequence in fragments shorter than    1908 bp
  95% of sequence in fragments shorter than    2594 bp

# BstNI.SspI (CCWGG+AATATT) double digest, 12232 thousand fragments (9855646+2377267 sites), 243 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     188 bp
  25% of sequence in fragments shorter than     229 bp
  30% of sequence in fragments shorter than     269 bp
  35% of sequence in fragments shorter than     312 bp
  40% of sequence in fragments shorter than     355 bp
  45% of sequence in fragments shorter than     402 bp
  50% of sequence in fragments shorter than     453 bp
  55% of sequence in fragments shorter than     507 bp
  60% of sequence in fragments shorter than     568 bp
  65% of sequence in fragments shorter than     635 bp
  70% of sequence in fragments shorter than     712 bp
  75% of sequence in fragments shorter than     803 bp
  80% of sequence in fragments shorter than     919 bp
  85% of sequence in fragments shorter than    1073 bp
  90% of sequence in fragments shorter than    1310 bp
  95% of sequence in fragments shorter than    1773 bp

# BthCI.HphI (GCNGC+GGTGA) double digest, 12216 thousand fragments (6209929+6007336 sites), 243 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     128 bp
  15% of sequence in fragments shorter than     167 bp
  20% of sequence in fragments shorter than     208 bp
  25% of sequence in fragments shorter than     249 bp
  30% of sequence in fragments shorter than     289 bp
  35% of sequence in fragments shorter than     332 bp
  40% of sequence in fragments shorter than     374 bp
  45% of sequence in fragments shorter than     423 bp
  50% of sequence in fragments shorter than     474 bp
  55% of sequence in fragments shorter than     530 bp
  60% of sequence in fragments shorter than     591 bp
  65% of sequence in fragments shorter than     661 bp
  70% of sequence in fragments shorter than     739 bp
  75% of sequence in fragments shorter than     833 bp
  80% of sequence in fragments shorter than     948 bp
  85% of sequence in fragments shorter than    1094 bp
  90% of sequence in fragments shorter than    1296 bp
  95% of sequence in fragments shorter than    1636 bp

# BscAI.HaeIII (GCATC+GGCC) double digest, 12214 thousand fragments (3652926+8562230 sites), 243 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     121 bp
  15% of sequence in fragments shorter than     166 bp
  20% of sequence in fragments shorter than     207 bp
  25% of sequence in fragments shorter than     251 bp
  30% of sequence in fragments shorter than     294 bp
  35% of sequence in fragments shorter than     339 bp
  40% of sequence in fragments shorter than     378 bp
  45% of sequence in fragments shorter than     427 bp
  50% of sequence in fragments shorter than     480 bp
  55% of sequence in fragments shorter than     535 bp
  60% of sequence in fragments shorter than     597 bp
  65% of sequence in fragments shorter than     669 bp
  70% of sequence in fragments shorter than     744 bp
  75% of sequence in fragments shorter than     840 bp
  80% of sequence in fragments shorter than     953 bp
  85% of sequence in fragments shorter than    1102 bp
  90% of sequence in fragments shorter than    1304 bp
  95% of sequence in fragments shorter than    1658 bp

# ScrFI.SpeI (CCNGG+ACTAGT) double digest, 12204 thousand fragments (11805099+400286 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     149 bp
  20% of sequence in fragments shorter than     199 bp
  25% of sequence in fragments shorter than     251 bp
  30% of sequence in fragments shorter than     306 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     427 bp
  45% of sequence in fragments shorter than     496 bp
  50% of sequence in fragments shorter than     572 bp
  55% of sequence in fragments shorter than     654 bp
  60% of sequence in fragments shorter than     744 bp
  65% of sequence in fragments shorter than     851 bp
  70% of sequence in fragments shorter than     977 bp
  75% of sequence in fragments shorter than    1126 bp
  80% of sequence in fragments shorter than    1313 bp
  85% of sequence in fragments shorter than    1545 bp
  90% of sequence in fragments shorter than    1903 bp
  95% of sequence in fragments shorter than    2594 bp

# HpaI.ScrFI (GTTAAC+CCNGG) double digest, 12194 thousand fragments (389418+11805099 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     149 bp
  20% of sequence in fragments shorter than     199 bp
  25% of sequence in fragments shorter than     250 bp
  30% of sequence in fragments shorter than     306 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     427 bp
  45% of sequence in fragments shorter than     497 bp
  50% of sequence in fragments shorter than     574 bp
  55% of sequence in fragments shorter than     656 bp
  60% of sequence in fragments shorter than     749 bp
  65% of sequence in fragments shorter than     858 bp
  70% of sequence in fragments shorter than     987 bp
  75% of sequence in fragments shorter than    1142 bp
  80% of sequence in fragments shorter than    1338 bp
  85% of sequence in fragments shorter than    1604 bp
  90% of sequence in fragments shorter than    1994 bp
  95% of sequence in fragments shorter than    2746 bp

# BspKT6I.TfiI (GATC+GAWTC) double digest, 12183 thousand fragments (7199434+4984362 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     160 bp
  20% of sequence in fragments shorter than     189 bp
  25% of sequence in fragments shorter than     226 bp
  30% of sequence in fragments shorter than     262 bp
  35% of sequence in fragments shorter than     297 bp
  40% of sequence in fragments shorter than     333 bp
  45% of sequence in fragments shorter than     365 bp
  50% of sequence in fragments shorter than     406 bp
  55% of sequence in fragments shorter than     450 bp
  60% of sequence in fragments shorter than     498 bp
  65% of sequence in fragments shorter than     552 bp
  70% of sequence in fragments shorter than     611 bp
  75% of sequence in fragments shorter than     678 bp
  80% of sequence in fragments shorter than     758 bp
  85% of sequence in fragments shorter than     861 bp
  90% of sequence in fragments shorter than    1006 bp
  95% of sequence in fragments shorter than    1248 bp

# SapI.ScrFI (GCTCTTC+CCNGG) double digest, 12181 thousand fragments (377161+11805099 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     149 bp
  20% of sequence in fragments shorter than     199 bp
  25% of sequence in fragments shorter than     251 bp
  30% of sequence in fragments shorter than     308 bp
  35% of sequence in fragments shorter than     368 bp
  40% of sequence in fragments shorter than     434 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     584 bp
  55% of sequence in fragments shorter than     669 bp
  60% of sequence in fragments shorter than     767 bp
  65% of sequence in fragments shorter than     880 bp
  70% of sequence in fragments shorter than    1014 bp
  75% of sequence in fragments shorter than    1179 bp
  80% of sequence in fragments shorter than    1383 bp
  85% of sequence in fragments shorter than    1664 bp
  90% of sequence in fragments shorter than    2068 bp
  95% of sequence in fragments shorter than    2838 bp

# ScrFI.SmaI (CCNGG+CCCGGG) double digest, 12181 thousand fragments (11805099+376940 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     531 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1466 bp
  85% of sequence in fragments shorter than    1763 bp
  90% of sequence in fragments shorter than    2183 bp
  95% of sequence in fragments shorter than    3008 bp

# BccI.HphI (CCATC+GGTGA) double digest, 12177 thousand fragments (6170927+6007336 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     243 bp
  30% of sequence in fragments shorter than     281 bp
  35% of sequence in fragments shorter than     320 bp
  40% of sequence in fragments shorter than     357 bp
  45% of sequence in fragments shorter than     401 bp
  50% of sequence in fragments shorter than     447 bp
  55% of sequence in fragments shorter than     495 bp
  60% of sequence in fragments shorter than     549 bp
  65% of sequence in fragments shorter than     610 bp
  70% of sequence in fragments shorter than     679 bp
  75% of sequence in fragments shorter than     760 bp
  80% of sequence in fragments shorter than     860 bp
  85% of sequence in fragments shorter than     991 bp
  90% of sequence in fragments shorter than    1164 bp
  95% of sequence in fragments shorter than    1481 bp

# CviQI.Hin4II (GTAC+CCTTC) double digest, 12174 thousand fragments (5115078+7059915 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     243 bp
  30% of sequence in fragments shorter than     280 bp
  35% of sequence in fragments shorter than     318 bp
  40% of sequence in fragments shorter than     354 bp
  45% of sequence in fragments shorter than     393 bp
  50% of sequence in fragments shorter than     437 bp
  55% of sequence in fragments shorter than     485 bp
  60% of sequence in fragments shorter than     534 bp
  65% of sequence in fragments shorter than     590 bp
  70% of sequence in fragments shorter than     652 bp
  75% of sequence in fragments shorter than     725 bp
  80% of sequence in fragments shorter than     813 bp
  85% of sequence in fragments shorter than     921 bp
  90% of sequence in fragments shorter than    1068 bp
  95% of sequence in fragments shorter than    1323 bp

# BstNI.HpaII (CCWGG+CCGG) double digest, 12172 thousand fragments (9855646+2317722 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      66 bp
  10% of sequence in fragments shorter than     104 bp
  15% of sequence in fragments shorter than     144 bp
  20% of sequence in fragments shorter than     194 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     303 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     429 bp
  45% of sequence in fragments shorter than     502 bp
  50% of sequence in fragments shorter than     582 bp
  55% of sequence in fragments shorter than     666 bp
  60% of sequence in fragments shorter than     766 bp
  65% of sequence in fragments shorter than     882 bp
  70% of sequence in fragments shorter than    1017 bp
  75% of sequence in fragments shorter than    1183 bp
  80% of sequence in fragments shorter than    1389 bp
  85% of sequence in fragments shorter than    1668 bp
  90% of sequence in fragments shorter than    2066 bp
  95% of sequence in fragments shorter than    2832 bp

# BamHI.ScrFI (GGATCC+CCNGG) double digest, 12170 thousand fragments (365999+11805099 sites), 244 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     254 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     373 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     512 bp
  50% of sequence in fragments shorter than     593 bp
  55% of sequence in fragments shorter than     678 bp
  60% of sequence in fragments shorter than     777 bp
  65% of sequence in fragments shorter than     888 bp
  70% of sequence in fragments shorter than    1022 bp
  75% of sequence in fragments shorter than    1181 bp
  80% of sequence in fragments shorter than    1381 bp
  85% of sequence in fragments shorter than    1650 bp
  90% of sequence in fragments shorter than    2046 bp
  95% of sequence in fragments shorter than    2812 bp

# BstEII.ScrFI (GGTNACC+CCNGG) double digest, 12140 thousand fragments (335865+11805099 sites), 245 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     253 bp
  30% of sequence in fragments shorter than     311 bp
  35% of sequence in fragments shorter than     372 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     512 bp
  50% of sequence in fragments shorter than     593 bp
  55% of sequence in fragments shorter than     678 bp
  60% of sequence in fragments shorter than     778 bp
  65% of sequence in fragments shorter than     894 bp
  70% of sequence in fragments shorter than    1030 bp
  75% of sequence in fragments shorter than    1198 bp
  80% of sequence in fragments shorter than    1405 bp
  85% of sequence in fragments shorter than    1687 bp
  90% of sequence in fragments shorter than    2098 bp
  95% of sequence in fragments shorter than    2903 bp

# ApoI.BsrI (RAATTY+ACTGG) double digest, 12123 thousand fragments (6382433+5741310 sites), 245 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     126 bp
  15% of sequence in fragments shorter than     163 bp
  20% of sequence in fragments shorter than     198 bp
  25% of sequence in fragments shorter than     233 bp
  30% of sequence in fragments shorter than     267 bp
  35% of sequence in fragments shorter than     303 bp
  40% of sequence in fragments shorter than     339 bp
  45% of sequence in fragments shorter than     378 bp
  50% of sequence in fragments shorter than     420 bp
  55% of sequence in fragments shorter than     465 bp
  60% of sequence in fragments shorter than     514 bp
  65% of sequence in fragments shorter than     568 bp
  70% of sequence in fragments shorter than     629 bp
  75% of sequence in fragments shorter than     698 bp
  80% of sequence in fragments shorter than     780 bp
  85% of sequence in fragments shorter than     890 bp
  90% of sequence in fragments shorter than    1045 bp
  95% of sequence in fragments shorter than    1322 bp

# ScrFI.SexAI (CCNGG+ACCWGGT) double digest, 12102 thousand fragments (11805099+297900 sites), 246 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     531 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1466 bp
  85% of sequence in fragments shorter than    1763 bp
  90% of sequence in fragments shorter than    2183 bp
  95% of sequence in fragments shorter than    3008 bp

# HaeIII.SfeI (GGCC+CTRYAG) double digest, 12092 thousand fragments (8562230+3531009 sites), 246 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     120 bp
  15% of sequence in fragments shorter than     164 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     250 bp
  30% of sequence in fragments shorter than     295 bp
  35% of sequence in fragments shorter than     342 bp
  40% of sequence in fragments shorter than     384 bp
  45% of sequence in fragments shorter than     435 bp
  50% of sequence in fragments shorter than     489 bp
  55% of sequence in fragments shorter than     547 bp
  60% of sequence in fragments shorter than     613 bp
  65% of sequence in fragments shorter than     683 bp
  70% of sequence in fragments shorter than     766 bp
  75% of sequence in fragments shorter than     864 bp
  80% of sequence in fragments shorter than     985 bp
  85% of sequence in fragments shorter than    1130 bp
  90% of sequence in fragments shorter than    1347 bp
  95% of sequence in fragments shorter than    1720 bp

# KpnI.ScrFI (GGTACC+CCNGG) double digest, 12092 thousand fragments (287974+11805099 sites), 246 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     202 bp
  25% of sequence in fragments shorter than     255 bp
  30% of sequence in fragments shorter than     314 bp
  35% of sequence in fragments shorter than     375 bp
  40% of sequence in fragments shorter than     441 bp
  45% of sequence in fragments shorter than     515 bp
  50% of sequence in fragments shorter than     596 bp
  55% of sequence in fragments shorter than     677 bp
  60% of sequence in fragments shorter than     776 bp
  65% of sequence in fragments shorter than     892 bp
  70% of sequence in fragments shorter than    1027 bp
  75% of sequence in fragments shorter than    1192 bp
  80% of sequence in fragments shorter than    1390 bp
  85% of sequence in fragments shorter than    1662 bp
  90% of sequence in fragments shorter than    2062 bp
  95% of sequence in fragments shorter than    2859 bp

# NheI.ScrFI (GCTAGC+CCNGG) double digest, 12092 thousand fragments (287384+11805099 sites), 246 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     110 bp
  15% of sequence in fragments shorter than     150 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     255 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     373 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     511 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     677 bp
  60% of sequence in fragments shorter than     775 bp
  65% of sequence in fragments shorter than     884 bp
  70% of sequence in fragments shorter than    1017 bp
  75% of sequence in fragments shorter than    1169 bp
  80% of sequence in fragments shorter than    1367 bp
  85% of sequence in fragments shorter than    1631 bp
  90% of sequence in fragments shorter than    2019 bp
  95% of sequence in fragments shorter than    2778 bp

# Hpy188I.StyI (TCNGA+CCWWGG) double digest, 12055 thousand fragments (8942153+3114107 sites), 247 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     127 bp
  15% of sequence in fragments shorter than     166 bp
  20% of sequence in fragments shorter than     196 bp
  25% of sequence in fragments shorter than     232 bp
  30% of sequence in fragments shorter than     268 bp
  35% of sequence in fragments shorter than     305 bp
  40% of sequence in fragments shorter than     343 bp
  45% of sequence in fragments shorter than     381 bp
  50% of sequence in fragments shorter than     422 bp
  55% of sequence in fragments shorter than     467 bp
  60% of sequence in fragments shorter than     519 bp
  65% of sequence in fragments shorter than     574 bp
  70% of sequence in fragments shorter than     635 bp
  75% of sequence in fragments shorter than     708 bp
  80% of sequence in fragments shorter than     796 bp
  85% of sequence in fragments shorter than     902 bp
  90% of sequence in fragments shorter than    1054 bp
  95% of sequence in fragments shorter than    1313 bp

# Hpy188I.NspI (TCNGA+RCATGY) double digest, 12046 thousand fragments (8942153+3104359 sites), 247 bp average fragment length
   5% of sequence in fragments shorter than      86 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     168 bp
  20% of sequence in fragments shorter than     198 bp
  25% of sequence in fragments shorter than     232 bp
  30% of sequence in fragments shorter than     268 bp
  35% of sequence in fragments shorter than     304 bp
  40% of sequence in fragments shorter than     342 bp
  45% of sequence in fragments shorter than     378 bp
  50% of sequence in fragments shorter than     419 bp
  55% of sequence in fragments shorter than     463 bp
  60% of sequence in fragments shorter than     513 bp
  65% of sequence in fragments shorter than     567 bp
  70% of sequence in fragments shorter than     627 bp
  75% of sequence in fragments shorter than     698 bp
  80% of sequence in fragments shorter than     784 bp
  85% of sequence in fragments shorter than     891 bp
  90% of sequence in fragments shorter than    1043 bp
  95% of sequence in fragments shorter than    1281 bp

# Hin4II.TfiI (CCTTC+GAWTC) double digest, 12043 thousand fragments (7059915+4984362 sites), 247 bp average fragment length
   5% of sequence in fragments shorter than      86 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     209 bp
  25% of sequence in fragments shorter than     245 bp
  30% of sequence in fragments shorter than     281 bp
  35% of sequence in fragments shorter than     317 bp
  40% of sequence in fragments shorter than     352 bp
  45% of sequence in fragments shorter than     393 bp
  50% of sequence in fragments shorter than     436 bp
  55% of sequence in fragments shorter than     484 bp
  60% of sequence in fragments shorter than     535 bp
  65% of sequence in fragments shorter than     592 bp
  70% of sequence in fragments shorter than     657 bp
  75% of sequence in fragments shorter than     732 bp
  80% of sequence in fragments shorter than     822 bp
  85% of sequence in fragments shorter than     937 bp
  90% of sequence in fragments shorter than    1095 bp
  95% of sequence in fragments shorter than    1363 bp

# BsrBI.ScrFI (CCGCTC+CCNGG) double digest, 12040 thousand fragments (235523+11805099 sites), 247 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     203 bp
  25% of sequence in fragments shorter than     257 bp
  30% of sequence in fragments shorter than     317 bp
  35% of sequence in fragments shorter than     378 bp
  40% of sequence in fragments shorter than     446 bp
  45% of sequence in fragments shorter than     521 bp
  50% of sequence in fragments shorter than     604 bp
  55% of sequence in fragments shorter than     693 bp
  60% of sequence in fragments shorter than     796 bp
  65% of sequence in fragments shorter than     917 bp
  70% of sequence in fragments shorter than    1057 bp
  75% of sequence in fragments shorter than    1230 bp
  80% of sequence in fragments shorter than    1445 bp
  85% of sequence in fragments shorter than    1737 bp
  90% of sequence in fragments shorter than    2147 bp
  95% of sequence in fragments shorter than    2952 bp

# KasI.ScrFI (GGCGCC+CCNGG) double digest, 12035 thousand fragments (230381+11805099 sites), 247 bp average fragment length
   5% of sequence in fragments shorter than      67 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     260 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     528 bp
  50% of sequence in fragments shorter than     611 bp
  55% of sequence in fragments shorter than     701 bp
  60% of sequence in fragments shorter than     805 bp
  65% of sequence in fragments shorter than     927 bp
  70% of sequence in fragments shorter than    1069 bp
  75% of sequence in fragments shorter than    1243 bp
  80% of sequence in fragments shorter than    1462 bp
  85% of sequence in fragments shorter than    1758 bp
  90% of sequence in fragments shorter than    2179 bp
  95% of sequence in fragments shorter than    3000 bp

# ScrFI.SwaI (CCNGG+ATTTAAAT) double digest, 12031 thousand fragments (11805099+226602 sites), 247 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     151 bp
  20% of sequence in fragments shorter than     203 bp
  25% of sequence in fragments shorter than     255 bp
  30% of sequence in fragments shorter than     313 bp
  35% of sequence in fragments shorter than     372 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     508 bp
  50% of sequence in fragments shorter than     587 bp
  55% of sequence in fragments shorter than     669 bp
  60% of sequence in fragments shorter than     765 bp
  65% of sequence in fragments shorter than     877 bp
  70% of sequence in fragments shorter than    1007 bp
  75% of sequence in fragments shorter than    1165 bp
  80% of sequence in fragments shorter than    1363 bp
  85% of sequence in fragments shorter than    1632 bp
  90% of sequence in fragments shorter than    2025 bp
  95% of sequence in fragments shorter than    2776 bp

# HinfI.StyI (GANTC+CCWWGG) double digest, 12029 thousand fragments (8916054+3114107 sites), 247 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     167 bp
  20% of sequence in fragments shorter than     202 bp
  25% of sequence in fragments shorter than     235 bp
  30% of sequence in fragments shorter than     272 bp
  35% of sequence in fragments shorter than     308 bp
  40% of sequence in fragments shorter than     342 bp
  45% of sequence in fragments shorter than     376 bp
  50% of sequence in fragments shorter than     419 bp
  55% of sequence in fragments shorter than     463 bp
  60% of sequence in fragments shorter than     512 bp
  65% of sequence in fragments shorter than     564 bp
  70% of sequence in fragments shorter than     627 bp
  75% of sequence in fragments shorter than     694 bp
  80% of sequence in fragments shorter than     779 bp
  85% of sequence in fragments shorter than     885 bp
  90% of sequence in fragments shorter than    1035 bp
  95% of sequence in fragments shorter than    1292 bp

# HinfI.NspI (GANTC+RCATGY) double digest, 12019 thousand fragments (8916054+3104359 sites), 247 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     203 bp
  25% of sequence in fragments shorter than     237 bp
  30% of sequence in fragments shorter than     272 bp
  35% of sequence in fragments shorter than     307 bp
  40% of sequence in fragments shorter than     341 bp
  45% of sequence in fragments shorter than     372 bp
  50% of sequence in fragments shorter than     409 bp
  55% of sequence in fragments shorter than     451 bp
  60% of sequence in fragments shorter than     497 bp
  65% of sequence in fragments shorter than     547 bp
  70% of sequence in fragments shorter than     605 bp
  75% of sequence in fragments shorter than     670 bp
  80% of sequence in fragments shorter than     746 bp
  85% of sequence in fragments shorter than     844 bp
  90% of sequence in fragments shorter than     983 bp
  95% of sequence in fragments shorter than    1209 bp

# AclI.ScrFI (AACGTT+CCNGG) double digest, 11977 thousand fragments (172706+11805099 sites), 248 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     257 bp
  30% of sequence in fragments shorter than     316 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     443 bp
  45% of sequence in fragments shorter than     517 bp
  50% of sequence in fragments shorter than     597 bp
  55% of sequence in fragments shorter than     683 bp
  60% of sequence in fragments shorter than     782 bp
  65% of sequence in fragments shorter than     897 bp
  70% of sequence in fragments shorter than    1031 bp
  75% of sequence in fragments shorter than    1192 bp
  80% of sequence in fragments shorter than    1393 bp
  85% of sequence in fragments shorter than    1665 bp
  90% of sequence in fragments shorter than    2052 bp
  95% of sequence in fragments shorter than    2784 bp

# PacI.ScrFI (TTAATTAA+CCNGG) double digest, 11964 thousand fragments (159455+11805099 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     257 bp
  30% of sequence in fragments shorter than     316 bp
  35% of sequence in fragments shorter than     377 bp
  40% of sequence in fragments shorter than     443 bp
  45% of sequence in fragments shorter than     517 bp
  50% of sequence in fragments shorter than     597 bp
  55% of sequence in fragments shorter than     683 bp
  60% of sequence in fragments shorter than     781 bp
  65% of sequence in fragments shorter than     897 bp
  70% of sequence in fragments shorter than    1032 bp
  75% of sequence in fragments shorter than    1196 bp
  80% of sequence in fragments shorter than    1400 bp
  85% of sequence in fragments shorter than    1678 bp
  90% of sequence in fragments shorter than    2081 bp
  95% of sequence in fragments shorter than    2866 bp

# Hpy99I.ScrFI (CGWCG+CCNGG) double digest, 11962 thousand fragments (157917+11805099 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     111 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     259 bp
  30% of sequence in fragments shorter than     319 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     449 bp
  45% of sequence in fragments shorter than     525 bp
  50% of sequence in fragments shorter than     607 bp
  55% of sequence in fragments shorter than     697 bp
  60% of sequence in fragments shorter than     800 bp
  65% of sequence in fragments shorter than     922 bp
  70% of sequence in fragments shorter than    1062 bp
  75% of sequence in fragments shorter than    1236 bp
  80% of sequence in fragments shorter than    1452 bp
  85% of sequence in fragments shorter than    1746 bp
  90% of sequence in fragments shorter than    2165 bp
  95% of sequence in fragments shorter than    2974 bp

# BsrI.BthCI (ACTGG+GCNGC) double digest, 11950 thousand fragments (5741310+6209929 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     175 bp
  20% of sequence in fragments shorter than     218 bp
  25% of sequence in fragments shorter than     261 bp
  30% of sequence in fragments shorter than     305 bp
  35% of sequence in fragments shorter than     351 bp
  40% of sequence in fragments shorter than     401 bp
  45% of sequence in fragments shorter than     451 bp
  50% of sequence in fragments shorter than     506 bp
  55% of sequence in fragments shorter than     566 bp
  60% of sequence in fragments shorter than     632 bp
  65% of sequence in fragments shorter than     702 bp
  70% of sequence in fragments shorter than     778 bp
  75% of sequence in fragments shorter than     870 bp
  80% of sequence in fragments shorter than     985 bp
  85% of sequence in fragments shorter than    1130 bp
  90% of sequence in fragments shorter than    1338 bp
  95% of sequence in fragments shorter than    1701 bp

# AvaII.MboII (GGWCC+GAAGA) double digest, 11946 thousand fragments (2747690+9199496 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     245 bp
  30% of sequence in fragments shorter than     284 bp
  35% of sequence in fragments shorter than     323 bp
  40% of sequence in fragments shorter than     364 bp
  45% of sequence in fragments shorter than     409 bp
  50% of sequence in fragments shorter than     456 bp
  55% of sequence in fragments shorter than     508 bp
  60% of sequence in fragments shorter than     564 bp
  65% of sequence in fragments shorter than     627 bp
  70% of sequence in fragments shorter than     696 bp
  75% of sequence in fragments shorter than     779 bp
  80% of sequence in fragments shorter than     877 bp
  85% of sequence in fragments shorter than     997 bp
  90% of sequence in fragments shorter than    1168 bp
  95% of sequence in fragments shorter than    1462 bp

# ScrFI.SnaBI (CCNGG+TACGTA) double digest, 11936 thousand fragments (11805099+132299 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     258 bp
  30% of sequence in fragments shorter than     317 bp
  35% of sequence in fragments shorter than     378 bp
  40% of sequence in fragments shorter than     445 bp
  45% of sequence in fragments shorter than     519 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     686 bp
  60% of sequence in fragments shorter than     786 bp
  65% of sequence in fragments shorter than     904 bp
  70% of sequence in fragments shorter than    1040 bp
  75% of sequence in fragments shorter than    1208 bp
  80% of sequence in fragments shorter than    1410 bp
  85% of sequence in fragments shorter than    1689 bp
  90% of sequence in fragments shorter than    2089 bp
  95% of sequence in fragments shorter than    2861 bp

# NaeI.ScrFI (GCCGGC+CCNGG) double digest, 11930 thousand fragments (125692+11805099 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     260 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     528 bp
  50% of sequence in fragments shorter than     610 bp
  55% of sequence in fragments shorter than     701 bp
  60% of sequence in fragments shorter than     805 bp
  65% of sequence in fragments shorter than     927 bp
  70% of sequence in fragments shorter than    1069 bp
  75% of sequence in fragments shorter than    1243 bp
  80% of sequence in fragments shorter than    1461 bp
  85% of sequence in fragments shorter than    1757 bp
  90% of sequence in fragments shorter than    2177 bp
  95% of sequence in fragments shorter than    2999 bp

# MboII.SmlI (GAAGA+CTYRAG) double digest, 11926 thousand fragments (9199496+2727867 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     135 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     243 bp
  30% of sequence in fragments shorter than     281 bp
  35% of sequence in fragments shorter than     319 bp
  40% of sequence in fragments shorter than     356 bp
  45% of sequence in fragments shorter than     398 bp
  50% of sequence in fragments shorter than     442 bp
  55% of sequence in fragments shorter than     489 bp
  60% of sequence in fragments shorter than     540 bp
  65% of sequence in fragments shorter than     598 bp
  70% of sequence in fragments shorter than     661 bp
  75% of sequence in fragments shorter than     736 bp
  80% of sequence in fragments shorter than     826 bp
  85% of sequence in fragments shorter than     931 bp
  90% of sequence in fragments shorter than    1084 bp
  95% of sequence in fragments shorter than    1344 bp

# ScrFI.XhoI (CCNGG+CTCGAG) double digest, 11925 thousand fragments (11805099+121308 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     152 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     259 bp
  30% of sequence in fragments shorter than     318 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     449 bp
  45% of sequence in fragments shorter than     525 bp
  50% of sequence in fragments shorter than     606 bp
  55% of sequence in fragments shorter than     696 bp
  60% of sequence in fragments shorter than     799 bp
  65% of sequence in fragments shorter than     920 bp
  70% of sequence in fragments shorter than    1061 bp
  75% of sequence in fragments shorter than    1235 bp
  80% of sequence in fragments shorter than    1451 bp
  85% of sequence in fragments shorter than    1744 bp
  90% of sequence in fragments shorter than    2163 bp
  95% of sequence in fragments shorter than    2977 bp

# BbvI.BsmAI (GCAGC+GTCTC) double digest, 11921 thousand fragments (5290051+6631585 sites), 249 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     168 bp
  20% of sequence in fragments shorter than     203 bp
  25% of sequence in fragments shorter than     247 bp
  30% of sequence in fragments shorter than     289 bp
  35% of sequence in fragments shorter than     333 bp
  40% of sequence in fragments shorter than     379 bp
  45% of sequence in fragments shorter than     430 bp
  50% of sequence in fragments shorter than     483 bp
  55% of sequence in fragments shorter than     537 bp
  60% of sequence in fragments shorter than     602 bp
  65% of sequence in fragments shorter than     674 bp
  70% of sequence in fragments shorter than     758 bp
  75% of sequence in fragments shorter than     857 bp
  80% of sequence in fragments shorter than     974 bp
  85% of sequence in fragments shorter than    1120 bp
  90% of sequence in fragments shorter than    1339 bp
  95% of sequence in fragments shorter than    1720 bp

# BccI.BsrI (CCATC+ACTGG) double digest, 11911 thousand fragments (6170927+5741310 sites), 250 bp average fragment length
   5% of sequence in fragments shorter than      86 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     203 bp
  25% of sequence in fragments shorter than     240 bp
  30% of sequence in fragments shorter than     278 bp
  35% of sequence in fragments shorter than     316 bp
  40% of sequence in fragments shorter than     355 bp
  45% of sequence in fragments shorter than     398 bp
  50% of sequence in fragments shorter than     443 bp
  55% of sequence in fragments shorter than     490 bp
  60% of sequence in fragments shorter than     543 bp
  65% of sequence in fragments shorter than     602 bp
  70% of sequence in fragments shorter than     668 bp
  75% of sequence in fragments shorter than     747 bp
  80% of sequence in fragments shorter than     843 bp
  85% of sequence in fragments shorter than     967 bp
  90% of sequence in fragments shorter than    1141 bp
  95% of sequence in fragments shorter than    1469 bp

# ClaI.ScrFI (ATCGAT+CCNGG) double digest, 11891 thousand fragments (86477+11805099 sites), 250 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     112 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     259 bp
  30% of sequence in fragments shorter than     319 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     449 bp
  45% of sequence in fragments shorter than     523 bp
  50% of sequence in fragments shorter than     604 bp
  55% of sequence in fragments shorter than     693 bp
  60% of sequence in fragments shorter than     795 bp
  65% of sequence in fragments shorter than     915 bp
  70% of sequence in fragments shorter than    1054 bp
  75% of sequence in fragments shorter than    1225 bp
  80% of sequence in fragments shorter than    1438 bp
  85% of sequence in fragments shorter than    1727 bp
  90% of sequence in fragments shorter than    2139 bp
  95% of sequence in fragments shorter than    2936 bp

# StyI.TspRI (CCWWGG+CASTG) double digest, 11878 thousand fragments (3114107+8765240 sites), 250 bp average fragment length
   5% of sequence in fragments shorter than      86 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     174 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     238 bp
  30% of sequence in fragments shorter than     277 bp
  35% of sequence in fragments shorter than     316 bp
  40% of sequence in fragments shorter than     355 bp
  45% of sequence in fragments shorter than     398 bp
  50% of sequence in fragments shorter than     444 bp
  55% of sequence in fragments shorter than     492 bp
  60% of sequence in fragments shorter than     547 bp
  65% of sequence in fragments shorter than     608 bp
  70% of sequence in fragments shorter than     678 bp
  75% of sequence in fragments shorter than     760 bp
  80% of sequence in fragments shorter than     862 bp
  85% of sequence in fragments shorter than     994 bp
  90% of sequence in fragments shorter than    1180 bp
  95% of sequence in fragments shorter than    1493 bp

# AatII.ScrFI (GACGTC+CCNGG) double digest, 11875 thousand fragments (71089+11805099 sites), 250 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     260 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     609 bp
  55% of sequence in fragments shorter than     698 bp
  60% of sequence in fragments shorter than     802 bp
  65% of sequence in fragments shorter than     923 bp
  70% of sequence in fragments shorter than    1064 bp
  75% of sequence in fragments shorter than    1238 bp
  80% of sequence in fragments shorter than    1454 bp
  85% of sequence in fragments shorter than    1749 bp
  90% of sequence in fragments shorter than    2167 bp
  95% of sequence in fragments shorter than    2982 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# SacII.ScrFI (CCGCGG+CCNGG) double digest, 11871 thousand fragments (66901+11805099 sites), 251 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     261 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     612 bp
  55% of sequence in fragments shorter than     703 bp
  60% of sequence in fragments shorter than     807 bp
  65% of sequence in fragments shorter than     929 bp
  70% of sequence in fragments shorter than    1072 bp
  75% of sequence in fragments shorter than    1246 bp
  80% of sequence in fragments shorter than    1465 bp
  85% of sequence in fragments shorter than    1761 bp
  90% of sequence in fragments shorter than    2182 bp
  95% of sequence in fragments shorter than    3006 bp

# NspI.TspRI (RCATGY+CASTG) double digest, 11869 thousand fragments (3104359+8765240 sites), 251 bp average fragment length
   5% of sequence in fragments shorter than      86 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     211 bp
  25% of sequence in fragments shorter than     242 bp
  30% of sequence in fragments shorter than     278 bp
  35% of sequence in fragments shorter than     315 bp
  40% of sequence in fragments shorter than     353 bp
  45% of sequence in fragments shorter than     394 bp
  50% of sequence in fragments shorter than     437 bp
  55% of sequence in fragments shorter than     484 bp
  60% of sequence in fragments shorter than     535 bp
  65% of sequence in fragments shorter than     592 bp
  70% of sequence in fragments shorter than     656 bp
  75% of sequence in fragments shorter than     733 bp
  80% of sequence in fragments shorter than     827 bp
  85% of sequence in fragments shorter than     946 bp
  90% of sequence in fragments shorter than    1115 bp
  95% of sequence in fragments shorter than    1416 bp

# PspXI.ScrFI (VCTCGAGB+CCNGG) double digest, 11863 thousand fragments (59193+11805099 sites), 251 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     260 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     528 bp
  50% of sequence in fragments shorter than     610 bp
  55% of sequence in fragments shorter than     700 bp
  60% of sequence in fragments shorter than     804 bp
  65% of sequence in fragments shorter than     926 bp
  70% of sequence in fragments shorter than    1068 bp
  75% of sequence in fragments shorter than    1241 bp
  80% of sequence in fragments shorter than    1459 bp
  85% of sequence in fragments shorter than    1754 bp
  90% of sequence in fragments shorter than    2173 bp
  95% of sequence in fragments shorter than    2990 bp

# AgeI.ScrFI (ACCGGT+CCNGG) double digest, 11858 thousand fragments (53386+11805099 sites), 251 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     261 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     608 bp
  55% of sequence in fragments shorter than     699 bp
  60% of sequence in fragments shorter than     803 bp
  65% of sequence in fragments shorter than     925 bp
  70% of sequence in fragments shorter than    1066 bp
  75% of sequence in fragments shorter than    1239 bp
  80% of sequence in fragments shorter than    1456 bp
  85% of sequence in fragments shorter than    1751 bp
  90% of sequence in fragments shorter than    2170 bp
  95% of sequence in fragments shorter than    2987 bp

# PmeI.ScrFI (GTTTAAAC+CCNGG) double digest, 11846 thousand fragments (42094+11805099 sites), 251 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     153 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     261 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     608 bp
  55% of sequence in fragments shorter than     698 bp
  60% of sequence in fragments shorter than     801 bp
  65% of sequence in fragments shorter than     922 bp
  70% of sequence in fragments shorter than    1062 bp
  75% of sequence in fragments shorter than    1235 bp
  80% of sequence in fragments shorter than    1449 bp
  85% of sequence in fragments shorter than    1742 bp
  90% of sequence in fragments shorter than    2159 bp
  95% of sequence in fragments shorter than    2968 bp

# SalI.ScrFI (GTCGAC+CCNGG) double digest, 11836 thousand fragments (31396+11805099 sites), 251 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     261 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     529 bp
  50% of sequence in fragments shorter than     611 bp
  55% of sequence in fragments shorter than     701 bp
  60% of sequence in fragments shorter than     805 bp
  65% of sequence in fragments shorter than     927 bp
  70% of sequence in fragments shorter than    1068 bp
  75% of sequence in fragments shorter than    1242 bp
  80% of sequence in fragments shorter than    1460 bp
  85% of sequence in fragments shorter than    1755 bp
  90% of sequence in fragments shorter than    2175 bp
  95% of sequence in fragments shorter than    2993 bp

# BsmAI.FokI (GTCTC+GGATG) double digest, 11832 thousand fragments (6631585+5201116 sites), 251 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     136 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     203 bp
  25% of sequence in fragments shorter than     242 bp
  30% of sequence in fragments shorter than     283 bp
  35% of sequence in fragments shorter than     324 bp
  40% of sequence in fragments shorter than     366 bp
  45% of sequence in fragments shorter than     411 bp
  50% of sequence in fragments shorter than     459 bp
  55% of sequence in fragments shorter than     510 bp
  60% of sequence in fragments shorter than     567 bp
  65% of sequence in fragments shorter than     630 bp
  70% of sequence in fragments shorter than     704 bp
  75% of sequence in fragments shorter than     792 bp
  80% of sequence in fragments shorter than     897 bp
  85% of sequence in fragments shorter than    1029 bp
  90% of sequence in fragments shorter than    1210 bp
  95% of sequence in fragments shorter than    1545 bp

# MluI.ScrFI (ACGCGT+CCNGG) double digest, 11826 thousand fragments (21415+11805099 sites), 251 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     261 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     612 bp
  55% of sequence in fragments shorter than     702 bp
  60% of sequence in fragments shorter than     807 bp
  65% of sequence in fragments shorter than     929 bp
  70% of sequence in fragments shorter than    1071 bp
  75% of sequence in fragments shorter than    1245 bp
  80% of sequence in fragments shorter than    1463 bp
  85% of sequence in fragments shorter than    1759 bp
  90% of sequence in fragments shorter than    2179 bp
  95% of sequence in fragments shorter than    3002 bp

# ScrFI.SgrAI (CCNGG+CRCCGGYG) double digest, 11819 thousand fragments (11805099+15270 sites), 252 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     703 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1072 bp
  75% of sequence in fragments shorter than    1246 bp
  80% of sequence in fragments shorter than    1465 bp
  85% of sequence in fragments shorter than    1762 bp
  90% of sequence in fragments shorter than    2182 bp
  95% of sequence in fragments shorter than    3007 bp

# NruI.ScrFI (TCGCGA+CCNGG) double digest, 11819 thousand fragments (14872+11805099 sites), 252 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     612 bp
  55% of sequence in fragments shorter than     703 bp
  60% of sequence in fragments shorter than     807 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1072 bp
  75% of sequence in fragments shorter than    1246 bp
  80% of sequence in fragments shorter than    1464 bp
  85% of sequence in fragments shorter than    1761 bp
  90% of sequence in fragments shorter than    2181 bp
  95% of sequence in fragments shorter than    3006 bp

# PvuI.ScrFI (CGATCG+CCNGG) double digest, 11818 thousand fragments (14186+11805099 sites), 252 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     612 bp
  55% of sequence in fragments shorter than     703 bp
  60% of sequence in fragments shorter than     807 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1072 bp
  75% of sequence in fragments shorter than    1246 bp
  80% of sequence in fragments shorter than    1464 bp
  85% of sequence in fragments shorter than    1759 bp
  90% of sequence in fragments shorter than    2173 bp
  95% of sequence in fragments shorter than    2980 bp

# FseI.ScrFI (GGCCGGCC+CCNGG) double digest, 11818 thousand fragments (13662+11805099 sites), 252 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     531 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1466 bp
  85% of sequence in fragments shorter than    1763 bp
  90% of sequence in fragments shorter than    2183 bp
  95% of sequence in fragments shorter than    3008 bp

# NotI.ScrFI (GCGGCCGC+CCNGG) double digest, 11814 thousand fragments (9795+11805099 sites), 252 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     531 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1466 bp
  85% of sequence in fragments shorter than    1763 bp
  90% of sequence in fragments shorter than    2183 bp
  95% of sequence in fragments shorter than    3008 bp

# RsrII.ScrFI (CGGWCCG+CCNGG) double digest, 11814 thousand fragments (9764+11805099 sites), 252 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     531 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1466 bp
  85% of sequence in fragments shorter than    1763 bp
  90% of sequence in fragments shorter than    2183 bp
  95% of sequence in fragments shorter than    3008 bp

# AscI.ScrFI (GGCGCGCC+CCNGG) double digest, 11809 thousand fragments (4751+11805099 sites), 252 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     531 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1466 bp
  85% of sequence in fragments shorter than    1763 bp
  90% of sequence in fragments shorter than    2183 bp
  95% of sequence in fragments shorter than    3008 bp

# BstNI.ScrFI (CCWGG+CCNGG) double digest, 11804 thousand fragments (9855646+11805099 sites), 252 bp average fragment length
   5% of sequence in fragments shorter than      68 bp
  10% of sequence in fragments shorter than     113 bp
  15% of sequence in fragments shorter than     154 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     531 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1466 bp
  85% of sequence in fragments shorter than    1763 bp
  90% of sequence in fragments shorter than    2183 bp
  95% of sequence in fragments shorter than    3008 bp

# BsrI.HphI (ACTGG+GGTGA) double digest, 11748 thousand fragments (5741310+6007336 sites), 253 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     207 bp
  25% of sequence in fragments shorter than     244 bp
  30% of sequence in fragments shorter than     281 bp
  35% of sequence in fragments shorter than     318 bp
  40% of sequence in fragments shorter than     355 bp
  45% of sequence in fragments shorter than     397 bp
  50% of sequence in fragments shorter than     440 bp
  55% of sequence in fragments shorter than     485 bp
  60% of sequence in fragments shorter than     537 bp
  65% of sequence in fragments shorter than     594 bp
  70% of sequence in fragments shorter than     659 bp
  75% of sequence in fragments shorter than     728 bp
  80% of sequence in fragments shorter than     820 bp
  85% of sequence in fragments shorter than     936 bp
  90% of sequence in fragments shorter than    1098 bp
  95% of sequence in fragments shorter than    1379 bp

# BsmAI.CviQI (GTCTC+GTAC) double digest, 11746 thousand fragments (6631585+5115078 sites), 253 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     136 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     202 bp
  25% of sequence in fragments shorter than     240 bp
  30% of sequence in fragments shorter than     278 bp
  35% of sequence in fragments shorter than     317 bp
  40% of sequence in fragments shorter than     357 bp
  45% of sequence in fragments shorter than     399 bp
  50% of sequence in fragments shorter than     442 bp
  55% of sequence in fragments shorter than     487 bp
  60% of sequence in fragments shorter than     537 bp
  65% of sequence in fragments shorter than     592 bp
  70% of sequence in fragments shorter than     655 bp
  75% of sequence in fragments shorter than     730 bp
  80% of sequence in fragments shorter than     821 bp
  85% of sequence in fragments shorter than     939 bp
  90% of sequence in fragments shorter than    1101 bp
  95% of sequence in fragments shorter than    1373 bp

# AvaII.Hpy188I (GGWCC+TCNGA) double digest, 11689 thousand fragments (2747690+8942153 sites), 254 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     204 bp
  25% of sequence in fragments shorter than     241 bp
  30% of sequence in fragments shorter than     281 bp
  35% of sequence in fragments shorter than     322 bp
  40% of sequence in fragments shorter than     361 bp
  45% of sequence in fragments shorter than     406 bp
  50% of sequence in fragments shorter than     454 bp
  55% of sequence in fragments shorter than     506 bp
  60% of sequence in fragments shorter than     563 bp
  65% of sequence in fragments shorter than     625 bp
  70% of sequence in fragments shorter than     698 bp
  75% of sequence in fragments shorter than     782 bp
  80% of sequence in fragments shorter than     877 bp
  85% of sequence in fragments shorter than    1002 bp
  90% of sequence in fragments shorter than    1177 bp
  95% of sequence in fragments shorter than    1471 bp

# HaeIII.StyI (GGCC+CCWWGG) double digest, 11675 thousand fragments (8562230+3114107 sites), 255 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     134 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     232 bp
  25% of sequence in fragments shorter than     282 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     428 bp
  45% of sequence in fragments shorter than     487 bp
  50% of sequence in fragments shorter than     545 bp
  55% of sequence in fragments shorter than     616 bp
  60% of sequence in fragments shorter than     691 bp
  65% of sequence in fragments shorter than     776 bp
  70% of sequence in fragments shorter than     875 bp
  75% of sequence in fragments shorter than     995 bp
  80% of sequence in fragments shorter than    1140 bp
  85% of sequence in fragments shorter than    1320 bp
  90% of sequence in fragments shorter than    1588 bp
  95% of sequence in fragments shorter than    2038 bp

# ApoI.BbvI (RAATTY+GCAGC) double digest, 11671 thousand fragments (6382433+5290051 sites), 255 bp average fragment length
   5% of sequence in fragments shorter than      88 bp
  10% of sequence in fragments shorter than     134 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     213 bp
  25% of sequence in fragments shorter than     251 bp
  30% of sequence in fragments shorter than     287 bp
  35% of sequence in fragments shorter than     324 bp
  40% of sequence in fragments shorter than     364 bp
  45% of sequence in fragments shorter than     406 bp
  50% of sequence in fragments shorter than     450 bp
  55% of sequence in fragments shorter than     498 bp
  60% of sequence in fragments shorter than     551 bp
  65% of sequence in fragments shorter than     608 bp
  70% of sequence in fragments shorter than     674 bp
  75% of sequence in fragments shorter than     748 bp
  80% of sequence in fragments shorter than     841 bp
  85% of sequence in fragments shorter than     953 bp
  90% of sequence in fragments shorter than    1113 bp
  95% of sequence in fragments shorter than    1367 bp

# Hpy188I.SmlI (TCNGA+CTYRAG) double digest, 11669 thousand fragments (8942153+2727867 sites), 255 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     202 bp
  25% of sequence in fragments shorter than     238 bp
  30% of sequence in fragments shorter than     276 bp
  35% of sequence in fragments shorter than     313 bp
  40% of sequence in fragments shorter than     349 bp
  45% of sequence in fragments shorter than     389 bp
  50% of sequence in fragments shorter than     432 bp
  55% of sequence in fragments shorter than     478 bp
  60% of sequence in fragments shorter than     529 bp
  65% of sequence in fragments shorter than     583 bp
  70% of sequence in fragments shorter than     645 bp
  75% of sequence in fragments shorter than     716 bp
  80% of sequence in fragments shorter than     803 bp
  85% of sequence in fragments shorter than     912 bp
  90% of sequence in fragments shorter than    1062 bp
  95% of sequence in fragments shorter than    1315 bp

# HaeIII.NspI (GGCC+RCATGY) double digest, 11666 thousand fragments (8562230+3104359 sites), 255 bp average fragment length
   5% of sequence in fragments shorter than      77 bp
  10% of sequence in fragments shorter than     128 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     219 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     304 bp
  35% of sequence in fragments shorter than     345 bp
  40% of sequence in fragments shorter than     390 bp
  45% of sequence in fragments shorter than     438 bp
  50% of sequence in fragments shorter than     492 bp
  55% of sequence in fragments shorter than     546 bp
  60% of sequence in fragments shorter than     612 bp
  65% of sequence in fragments shorter than     684 bp
  70% of sequence in fragments shorter than     764 bp
  75% of sequence in fragments shorter than     859 bp
  80% of sequence in fragments shorter than     971 bp
  85% of sequence in fragments shorter than    1119 bp
  90% of sequence in fragments shorter than    1317 bp
  95% of sequence in fragments shorter than    1671 bp

# AvaII.HinfI (GGWCC+GANTC) double digest, 11663 thousand fragments (2747690+8916054 sites), 255 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     172 bp
  20% of sequence in fragments shorter than     210 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     284 bp
  35% of sequence in fragments shorter than     320 bp
  40% of sequence in fragments shorter than     352 bp
  45% of sequence in fragments shorter than     392 bp
  50% of sequence in fragments shorter than     436 bp
  55% of sequence in fragments shorter than     484 bp
  60% of sequence in fragments shorter than     535 bp
  65% of sequence in fragments shorter than     593 bp
  70% of sequence in fragments shorter than     658 bp
  75% of sequence in fragments shorter than     728 bp
  80% of sequence in fragments shorter than     817 bp
  85% of sequence in fragments shorter than     931 bp
  90% of sequence in fragments shorter than    1090 bp
  95% of sequence in fragments shorter than    1359 bp

# HinfI.SmlI (GANTC+CTYRAG) double digest, 11643 thousand fragments (8916054+2727867 sites), 255 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     134 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     210 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     282 bp
  35% of sequence in fragments shorter than     318 bp
  40% of sequence in fragments shorter than     349 bp
  45% of sequence in fragments shorter than     388 bp
  50% of sequence in fragments shorter than     431 bp
  55% of sequence in fragments shorter than     476 bp
  60% of sequence in fragments shorter than     523 bp
  65% of sequence in fragments shorter than     578 bp
  70% of sequence in fragments shorter than     640 bp
  75% of sequence in fragments shorter than     707 bp
  80% of sequence in fragments shorter than     792 bp
  85% of sequence in fragments shorter than     900 bp
  90% of sequence in fragments shorter than    1046 bp
  95% of sequence in fragments shorter than    1300 bp

# BsmAI.TfiI (GTCTC+GAWTC) double digest, 11615 thousand fragments (6631585+4984362 sites), 256 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     205 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     286 bp
  35% of sequence in fragments shorter than     326 bp
  40% of sequence in fragments shorter than     362 bp
  45% of sequence in fragments shorter than     404 bp
  50% of sequence in fragments shorter than     451 bp
  55% of sequence in fragments shorter than     502 bp
  60% of sequence in fragments shorter than     556 bp
  65% of sequence in fragments shorter than     616 bp
  70% of sequence in fragments shorter than     684 bp
  75% of sequence in fragments shorter than     761 bp
  80% of sequence in fragments shorter than     856 bp
  85% of sequence in fragments shorter than     975 bp
  90% of sequence in fragments shorter than    1137 bp
  95% of sequence in fragments shorter than    1416 bp

# BstNI.HhaI (CCWGG+GCGC) double digest, 11611 thousand fragments (9855646+1756502 sites), 256 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     117 bp
  15% of sequence in fragments shorter than     161 bp
  20% of sequence in fragments shorter than     210 bp
  25% of sequence in fragments shorter than     265 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     447 bp
  45% of sequence in fragments shorter than     519 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     685 bp
  60% of sequence in fragments shorter than     775 bp
  65% of sequence in fragments shorter than     889 bp
  70% of sequence in fragments shorter than    1023 bp
  75% of sequence in fragments shorter than    1179 bp
  80% of sequence in fragments shorter than    1375 bp
  85% of sequence in fragments shorter than    1632 bp
  90% of sequence in fragments shorter than    1978 bp
  95% of sequence in fragments shorter than    2587 bp

# ApoI.FokI (RAATTY+GGATG) double digest, 11583 thousand fragments (6382433+5201116 sites), 257 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     134 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     212 bp
  25% of sequence in fragments shorter than     248 bp
  30% of sequence in fragments shorter than     284 bp
  35% of sequence in fragments shorter than     321 bp
  40% of sequence in fragments shorter than     357 bp
  45% of sequence in fragments shorter than     398 bp
  50% of sequence in fragments shorter than     441 bp
  55% of sequence in fragments shorter than     488 bp
  60% of sequence in fragments shorter than     538 bp
  65% of sequence in fragments shorter than     593 bp
  70% of sequence in fragments shorter than     652 bp
  75% of sequence in fragments shorter than     722 bp
  80% of sequence in fragments shorter than     806 bp
  85% of sequence in fragments shorter than     914 bp
  90% of sequence in fragments shorter than    1067 bp
  95% of sequence in fragments shorter than    1329 bp

# MboII.SspI (GAAGA+AATATT) double digest, 11576 thousand fragments (9199496+2377267 sites), 257 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     136 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     212 bp
  25% of sequence in fragments shorter than     252 bp
  30% of sequence in fragments shorter than     294 bp
  35% of sequence in fragments shorter than     334 bp
  40% of sequence in fragments shorter than     375 bp
  45% of sequence in fragments shorter than     422 bp
  50% of sequence in fragments shorter than     469 bp
  55% of sequence in fragments shorter than     520 bp
  60% of sequence in fragments shorter than     576 bp
  65% of sequence in fragments shorter than     639 bp
  70% of sequence in fragments shorter than     711 bp
  75% of sequence in fragments shorter than     794 bp
  80% of sequence in fragments shorter than     894 bp
  85% of sequence in fragments shorter than    1021 bp
  90% of sequence in fragments shorter than    1198 bp
  95% of sequence in fragments shorter than    1514 bp

# BfiI.BstNI (ACTGGG+CCWGG) double digest, 11561 thousand fragments (1706594+9855646 sites), 257 bp average fragment length
   5% of sequence in fragments shorter than      72 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     159 bp
  20% of sequence in fragments shorter than     202 bp
  25% of sequence in fragments shorter than     249 bp
  30% of sequence in fragments shorter than     298 bp
  35% of sequence in fragments shorter than     349 bp
  40% of sequence in fragments shorter than     406 bp
  45% of sequence in fragments shorter than     469 bp
  50% of sequence in fragments shorter than     539 bp
  55% of sequence in fragments shorter than     616 bp
  60% of sequence in fragments shorter than     699 bp
  65% of sequence in fragments shorter than     795 bp
  70% of sequence in fragments shorter than     912 bp
  75% of sequence in fragments shorter than    1048 bp
  80% of sequence in fragments shorter than    1224 bp
  85% of sequence in fragments shorter than    1458 bp
  90% of sequence in fragments shorter than    1770 bp
  95% of sequence in fragments shorter than    2403 bp

# HpaII.MboII (CCGG+GAAGA) double digest, 11516 thousand fragments (2317722+9199496 sites), 258 bp average fragment length
   5% of sequence in fragments shorter than      90 bp
  10% of sequence in fragments shorter than     139 bp
  15% of sequence in fragments shorter than     175 bp
  20% of sequence in fragments shorter than     215 bp
  25% of sequence in fragments shorter than     255 bp
  30% of sequence in fragments shorter than     296 bp
  35% of sequence in fragments shorter than     335 bp
  40% of sequence in fragments shorter than     376 bp
  45% of sequence in fragments shorter than     420 bp
  50% of sequence in fragments shorter than     466 bp
  55% of sequence in fragments shorter than     516 bp
  60% of sequence in fragments shorter than     571 bp
  65% of sequence in fragments shorter than     632 bp
  70% of sequence in fragments shorter than     700 bp
  75% of sequence in fragments shorter than     780 bp
  80% of sequence in fragments shorter than     872 bp
  85% of sequence in fragments shorter than     991 bp
  90% of sequence in fragments shorter than    1150 bp
  95% of sequence in fragments shorter than    1425 bp

# AvaII.TspRI (GGWCC+CASTG) double digest, 11512 thousand fragments (2747690+8765240 sites), 258 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     138 bp
  15% of sequence in fragments shorter than     181 bp
  20% of sequence in fragments shorter than     216 bp
  25% of sequence in fragments shorter than     253 bp
  30% of sequence in fragments shorter than     293 bp
  35% of sequence in fragments shorter than     333 bp
  40% of sequence in fragments shorter than     372 bp
  45% of sequence in fragments shorter than     417 bp
  50% of sequence in fragments shorter than     465 bp
  55% of sequence in fragments shorter than     516 bp
  60% of sequence in fragments shorter than     572 bp
  65% of sequence in fragments shorter than     637 bp
  70% of sequence in fragments shorter than     707 bp
  75% of sequence in fragments shorter than     794 bp
  80% of sequence in fragments shorter than     899 bp
  85% of sequence in fragments shorter than    1021 bp
  90% of sequence in fragments shorter than    1208 bp
  95% of sequence in fragments shorter than    1555 bp

# BstNI.PsiI (CCWGG+TTATAA) double digest, 11503 thousand fragments (9855646+1647912 sites), 259 bp average fragment length
   5% of sequence in fragments shorter than      77 bp
  10% of sequence in fragments shorter than     119 bp
  15% of sequence in fragments shorter than     162 bp
  20% of sequence in fragments shorter than     202 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     291 bp
  35% of sequence in fragments shorter than     337 bp
  40% of sequence in fragments shorter than     386 bp
  45% of sequence in fragments shorter than     439 bp
  50% of sequence in fragments shorter than     496 bp
  55% of sequence in fragments shorter than     559 bp
  60% of sequence in fragments shorter than     629 bp
  65% of sequence in fragments shorter than     705 bp
  70% of sequence in fragments shorter than     795 bp
  75% of sequence in fragments shorter than     904 bp
  80% of sequence in fragments shorter than    1039 bp
  85% of sequence in fragments shorter than    1220 bp
  90% of sequence in fragments shorter than    1491 bp
  95% of sequence in fragments shorter than    2039 bp

# ApoI.CviQI (RAATTY+GTAC) double digest, 11497 thousand fragments (6382433+5115078 sites), 259 bp average fragment length
   5% of sequence in fragments shorter than      86 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     172 bp
  20% of sequence in fragments shorter than     211 bp
  25% of sequence in fragments shorter than     250 bp
  30% of sequence in fragments shorter than     288 bp
  35% of sequence in fragments shorter than     329 bp
  40% of sequence in fragments shorter than     370 bp
  45% of sequence in fragments shorter than     414 bp
  50% of sequence in fragments shorter than     460 bp
  55% of sequence in fragments shorter than     511 bp
  60% of sequence in fragments shorter than     567 bp
  65% of sequence in fragments shorter than     631 bp
  70% of sequence in fragments shorter than     704 bp
  75% of sequence in fragments shorter than     789 bp
  80% of sequence in fragments shorter than     883 bp
  85% of sequence in fragments shorter than    1020 bp
  90% of sequence in fragments shorter than    1219 bp
  95% of sequence in fragments shorter than    1591 bp

# SmlI.TspRI (CTYRAG+CASTG) double digest, 11492 thousand fragments (2727867+8765240 sites), 259 bp average fragment length
   5% of sequence in fragments shorter than      91 bp
  10% of sequence in fragments shorter than     138 bp
  15% of sequence in fragments shorter than     175 bp
  20% of sequence in fragments shorter than     214 bp
  25% of sequence in fragments shorter than     248 bp
  30% of sequence in fragments shorter than     288 bp
  35% of sequence in fragments shorter than     327 bp
  40% of sequence in fragments shorter than     367 bp
  45% of sequence in fragments shorter than     410 bp
  50% of sequence in fragments shorter than     455 bp
  55% of sequence in fragments shorter than     506 bp
  60% of sequence in fragments shorter than     559 bp
  65% of sequence in fragments shorter than     620 bp
  70% of sequence in fragments shorter than     689 bp
  75% of sequence in fragments shorter than     770 bp
  80% of sequence in fragments shorter than     870 bp
  85% of sequence in fragments shorter than     991 bp
  90% of sequence in fragments shorter than    1169 bp
  95% of sequence in fragments shorter than    1474 bp

# AciI.BspNCI (CCGC+CCAGA) double digest, 11488 thousand fragments (4206130+7282436 sites), 259 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     135 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     225 bp
  25% of sequence in fragments shorter than     272 bp
  30% of sequence in fragments shorter than     318 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     414 bp
  45% of sequence in fragments shorter than     466 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     580 bp
  60% of sequence in fragments shorter than     643 bp
  65% of sequence in fragments shorter than     715 bp
  70% of sequence in fragments shorter than     800 bp
  75% of sequence in fragments shorter than     898 bp
  80% of sequence in fragments shorter than    1020 bp
  85% of sequence in fragments shorter than    1163 bp
  90% of sequence in fragments shorter than    1380 bp
  95% of sequence in fragments shorter than    1752 bp

# BbvI.BccI (GCAGC+CCATC) double digest, 11460 thousand fragments (5290051+6170927 sites), 260 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     136 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     219 bp
  25% of sequence in fragments shorter than     261 bp
  30% of sequence in fragments shorter than     305 bp
  35% of sequence in fragments shorter than     348 bp
  40% of sequence in fragments shorter than     395 bp
  45% of sequence in fragments shorter than     445 bp
  50% of sequence in fragments shorter than     499 bp
  55% of sequence in fragments shorter than     558 bp
  60% of sequence in fragments shorter than     622 bp
  65% of sequence in fragments shorter than     694 bp
  70% of sequence in fragments shorter than     780 bp
  75% of sequence in fragments shorter than     878 bp
  80% of sequence in fragments shorter than    1001 bp
  85% of sequence in fragments shorter than    1149 bp
  90% of sequence in fragments shorter than    1369 bp
  95% of sequence in fragments shorter than    1764 bp

# BstNI.EsaBC3I (CCWGG+TCGA) double digest, 11458 thousand fragments (9855646+1603346 sites), 260 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     119 bp
  15% of sequence in fragments shorter than     163 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     256 bp
  30% of sequence in fragments shorter than     308 bp
  35% of sequence in fragments shorter than     363 bp
  40% of sequence in fragments shorter than     422 bp
  45% of sequence in fragments shorter than     486 bp
  50% of sequence in fragments shorter than     556 bp
  55% of sequence in fragments shorter than     632 bp
  60% of sequence in fragments shorter than     718 bp
  65% of sequence in fragments shorter than     817 bp
  70% of sequence in fragments shorter than     933 bp
  75% of sequence in fragments shorter than    1073 bp
  80% of sequence in fragments shorter than    1247 bp
  85% of sequence in fragments shorter than    1469 bp
  90% of sequence in fragments shorter than    1807 bp
  95% of sequence in fragments shorter than    2378 bp

# BthCI.FokI (GCNGC+GGATG) double digest, 11410 thousand fragments (6209929+5201116 sites), 261 bp average fragment length
   5% of sequence in fragments shorter than      86 bp
  10% of sequence in fragments shorter than     138 bp
  15% of sequence in fragments shorter than     184 bp
  20% of sequence in fragments shorter than     231 bp
  25% of sequence in fragments shorter than     277 bp
  30% of sequence in fragments shorter than     325 bp
  35% of sequence in fragments shorter than     373 bp
  40% of sequence in fragments shorter than     427 bp
  45% of sequence in fragments shorter than     483 bp
  50% of sequence in fragments shorter than     543 bp
  55% of sequence in fragments shorter than     607 bp
  60% of sequence in fragments shorter than     678 bp
  65% of sequence in fragments shorter than     758 bp
  70% of sequence in fragments shorter than     845 bp
  75% of sequence in fragments shorter than     949 bp
  80% of sequence in fragments shorter than    1075 bp
  85% of sequence in fragments shorter than    1238 bp
  90% of sequence in fragments shorter than    1456 bp
  95% of sequence in fragments shorter than    1842 bp

# BstNI.HgiCI (CCWGG+GGYRCC) double digest, 11406 thousand fragments (9855646+1550877 sites), 261 bp average fragment length
   5% of sequence in fragments shorter than      70 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     164 bp
  20% of sequence in fragments shorter than     212 bp
  25% of sequence in fragments shorter than     265 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     380 bp
  40% of sequence in fragments shorter than     443 bp
  45% of sequence in fragments shorter than     514 bp
  50% of sequence in fragments shorter than     592 bp
  55% of sequence in fragments shorter than     670 bp
  60% of sequence in fragments shorter than     764 bp
  65% of sequence in fragments shorter than     876 bp
  70% of sequence in fragments shorter than    1007 bp
  75% of sequence in fragments shorter than    1166 bp
  80% of sequence in fragments shorter than    1360 bp
  85% of sequence in fragments shorter than    1623 bp
  90% of sequence in fragments shorter than    2013 bp
  95% of sequence in fragments shorter than    2785 bp

# AciI.BspKT6I (CCGC+GATC) double digest, 11405 thousand fragments (4206130+7199434 sites), 261 bp average fragment length
   5% of sequence in fragments shorter than      90 bp
  10% of sequence in fragments shorter than     143 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     226 bp
  25% of sequence in fragments shorter than     275 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     368 bp
  40% of sequence in fragments shorter than     418 bp
  45% of sequence in fragments shorter than     468 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     582 bp
  60% of sequence in fragments shorter than     647 bp
  65% of sequence in fragments shorter than     720 bp
  70% of sequence in fragments shorter than     804 bp
  75% of sequence in fragments shorter than     896 bp
  80% of sequence in fragments shorter than    1012 bp
  85% of sequence in fragments shorter than    1156 bp
  90% of sequence in fragments shorter than    1366 bp
  95% of sequence in fragments shorter than    1714 bp

# BsmI.BstNI (GAATGC+CCWGG) double digest, 11404 thousand fragments (1549350+9855646 sites), 261 bp average fragment length
   5% of sequence in fragments shorter than      77 bp
  10% of sequence in fragments shorter than     120 bp
  15% of sequence in fragments shorter than     163 bp
  20% of sequence in fragments shorter than     201 bp
  25% of sequence in fragments shorter than     248 bp
  30% of sequence in fragments shorter than     298 bp
  35% of sequence in fragments shorter than     348 bp
  40% of sequence in fragments shorter than     404 bp
  45% of sequence in fragments shorter than     466 bp
  50% of sequence in fragments shorter than     531 bp
  55% of sequence in fragments shorter than     604 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     778 bp
  70% of sequence in fragments shorter than     886 bp
  75% of sequence in fragments shorter than    1014 bp
  80% of sequence in fragments shorter than    1174 bp
  85% of sequence in fragments shorter than    1381 bp
  90% of sequence in fragments shorter than    1680 bp
  95% of sequence in fragments shorter than    2178 bp

# BccI.FokI (CCATC+GGATG) double digest, 11371 thousand fragments (6170927+5201116 sites), 262 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     300 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     441 bp
  45% of sequence in fragments shorter than     493 bp
  50% of sequence in fragments shorter than     549 bp
  55% of sequence in fragments shorter than     609 bp
  60% of sequence in fragments shorter than     676 bp
  65% of sequence in fragments shorter than     748 bp
  70% of sequence in fragments shorter than     831 bp
  75% of sequence in fragments shorter than     928 bp
  80% of sequence in fragments shorter than    1048 bp
  85% of sequence in fragments shorter than    1195 bp
  90% of sequence in fragments shorter than    1404 bp
  95% of sequence in fragments shorter than    1783 bp

# ApoI.TfiI (RAATTY+GAWTC) double digest, 11366 thousand fragments (6382433+4984362 sites), 262 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     135 bp
  15% of sequence in fragments shorter than     174 bp
  20% of sequence in fragments shorter than     212 bp
  25% of sequence in fragments shorter than     251 bp
  30% of sequence in fragments shorter than     289 bp
  35% of sequence in fragments shorter than     326 bp
  40% of sequence in fragments shorter than     361 bp
  45% of sequence in fragments shorter than     405 bp
  50% of sequence in fragments shorter than     453 bp
  55% of sequence in fragments shorter than     502 bp
  60% of sequence in fragments shorter than     558 bp
  65% of sequence in fragments shorter than     619 bp
  70% of sequence in fragments shorter than     688 bp
  75% of sequence in fragments shorter than     772 bp
  80% of sequence in fragments shorter than     873 bp
  85% of sequence in fragments shorter than    1005 bp
  90% of sequence in fragments shorter than    1195 bp
  95% of sequence in fragments shorter than    1543 bp

# AflIII.BstNI (ACRYGT+CCWGG) double digest, 11340 thousand fragments (1485394+9855646 sites), 262 bp average fragment length
   5% of sequence in fragments shorter than      76 bp
  10% of sequence in fragments shorter than     120 bp
  15% of sequence in fragments shorter than     164 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     253 bp
  30% of sequence in fragments shorter than     302 bp
  35% of sequence in fragments shorter than     352 bp
  40% of sequence in fragments shorter than     404 bp
  45% of sequence in fragments shorter than     463 bp
  50% of sequence in fragments shorter than     528 bp
  55% of sequence in fragments shorter than     598 bp
  60% of sequence in fragments shorter than     675 bp
  65% of sequence in fragments shorter than     762 bp
  70% of sequence in fragments shorter than     867 bp
  75% of sequence in fragments shorter than     991 bp
  80% of sequence in fragments shorter than    1145 bp
  85% of sequence in fragments shorter than    1349 bp
  90% of sequence in fragments shorter than    1636 bp
  95% of sequence in fragments shorter than    2158 bp

# BthCI.CviQI (GCNGC+GTAC) double digest, 11324 thousand fragments (6209929+5115078 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     141 bp
  15% of sequence in fragments shorter than     188 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     272 bp
  30% of sequence in fragments shorter than     315 bp
  35% of sequence in fragments shorter than     357 bp
  40% of sequence in fragments shorter than     405 bp
  45% of sequence in fragments shorter than     453 bp
  50% of sequence in fragments shorter than     505 bp
  55% of sequence in fragments shorter than     558 bp
  60% of sequence in fragments shorter than     617 bp
  65% of sequence in fragments shorter than     683 bp
  70% of sequence in fragments shorter than     758 bp
  75% of sequence in fragments shorter than     846 bp
  80% of sequence in fragments shorter than     946 bp
  85% of sequence in fragments shorter than    1073 bp
  90% of sequence in fragments shorter than    1257 bp
  95% of sequence in fragments shorter than    1565 bp

# Hpy188I.SspI (TCNGA+AATATT) double digest, 11318 thousand fragments (8942153+2377267 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     135 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     210 bp
  25% of sequence in fragments shorter than     248 bp
  30% of sequence in fragments shorter than     289 bp
  35% of sequence in fragments shorter than     329 bp
  40% of sequence in fragments shorter than     367 bp
  45% of sequence in fragments shorter than     411 bp
  50% of sequence in fragments shorter than     457 bp
  55% of sequence in fragments shorter than     508 bp
  60% of sequence in fragments shorter than     562 bp
  65% of sequence in fragments shorter than     623 bp
  70% of sequence in fragments shorter than     693 bp
  75% of sequence in fragments shorter than     774 bp
  80% of sequence in fragments shorter than     868 bp
  85% of sequence in fragments shorter than     989 bp
  90% of sequence in fragments shorter than    1158 bp
  95% of sequence in fragments shorter than    1432 bp

# AvaII.HaeIII (GGWCC+GGCC) double digest, 11309 thousand fragments (2747690+8562230 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      76 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     177 bp
  20% of sequence in fragments shorter than     228 bp
  25% of sequence in fragments shorter than     280 bp
  30% of sequence in fragments shorter than     331 bp
  35% of sequence in fragments shorter than     377 bp
  40% of sequence in fragments shorter than     435 bp
  45% of sequence in fragments shorter than     497 bp
  50% of sequence in fragments shorter than     562 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     715 bp
  65% of sequence in fragments shorter than     807 bp
  70% of sequence in fragments shorter than     905 bp
  75% of sequence in fragments shorter than    1029 bp
  80% of sequence in fragments shorter than    1175 bp
  85% of sequence in fragments shorter than    1373 bp
  90% of sequence in fragments shorter than    1652 bp
  95% of sequence in fragments shorter than    2140 bp

# BspNCI.Tsp45I (CCAGA+GTSAC) double digest, 11303 thousand fragments (7282436+4021761 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     135 bp
  15% of sequence in fragments shorter than     177 bp
  20% of sequence in fragments shorter than     217 bp
  25% of sequence in fragments shorter than     257 bp
  30% of sequence in fragments shorter than     297 bp
  35% of sequence in fragments shorter than     338 bp
  40% of sequence in fragments shorter than     379 bp
  45% of sequence in fragments shorter than     424 bp
  50% of sequence in fragments shorter than     471 bp
  55% of sequence in fragments shorter than     522 bp
  60% of sequence in fragments shorter than     576 bp
  65% of sequence in fragments shorter than     635 bp
  70% of sequence in fragments shorter than     705 bp
  75% of sequence in fragments shorter than     787 bp
  80% of sequence in fragments shorter than     887 bp
  85% of sequence in fragments shorter than    1016 bp
  90% of sequence in fragments shorter than    1184 bp
  95% of sequence in fragments shorter than    1499 bp

# BbvI.HphI (GCAGC+GGTGA) double digest, 11296 thousand fragments (5290051+6007336 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     137 bp
  15% of sequence in fragments shorter than     177 bp
  20% of sequence in fragments shorter than     219 bp
  25% of sequence in fragments shorter than     260 bp
  30% of sequence in fragments shorter than     302 bp
  35% of sequence in fragments shorter than     344 bp
  40% of sequence in fragments shorter than     390 bp
  45% of sequence in fragments shorter than     440 bp
  50% of sequence in fragments shorter than     491 bp
  55% of sequence in fragments shorter than     549 bp
  60% of sequence in fragments shorter than     611 bp
  65% of sequence in fragments shorter than     682 bp
  70% of sequence in fragments shorter than     762 bp
  75% of sequence in fragments shorter than     860 bp
  80% of sequence in fragments shorter than     980 bp
  85% of sequence in fragments shorter than    1133 bp
  90% of sequence in fragments shorter than    1335 bp
  95% of sequence in fragments shorter than    1696 bp

# BstNI.FauI (CCWGG+CCCGC) double digest, 11294 thousand fragments (9855646+1439772 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      69 bp
  10% of sequence in fragments shorter than     118 bp
  15% of sequence in fragments shorter than     163 bp
  20% of sequence in fragments shorter than     212 bp
  25% of sequence in fragments shorter than     269 bp
  30% of sequence in fragments shorter than     328 bp
  35% of sequence in fragments shorter than     389 bp
  40% of sequence in fragments shorter than     458 bp
  45% of sequence in fragments shorter than     534 bp
  50% of sequence in fragments shorter than     614 bp
  55% of sequence in fragments shorter than     702 bp
  60% of sequence in fragments shorter than     806 bp
  65% of sequence in fragments shorter than     926 bp
  70% of sequence in fragments shorter than    1065 bp
  75% of sequence in fragments shorter than    1236 bp
  80% of sequence in fragments shorter than    1441 bp
  85% of sequence in fragments shorter than    1723 bp
  90% of sequence in fragments shorter than    2111 bp
  95% of sequence in fragments shorter than    2856 bp

# BspMI.BstNI (ACCTGC+CCWGG) double digest, 11293 thousand fragments (1438129+9855646 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      73 bp
  10% of sequence in fragments shorter than     119 bp
  15% of sequence in fragments shorter than     164 bp
  20% of sequence in fragments shorter than     208 bp
  25% of sequence in fragments shorter than     259 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     491 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     644 bp
  60% of sequence in fragments shorter than     728 bp
  65% of sequence in fragments shorter than     832 bp
  70% of sequence in fragments shorter than     949 bp
  75% of sequence in fragments shorter than    1093 bp
  80% of sequence in fragments shorter than    1273 bp
  85% of sequence in fragments shorter than    1505 bp
  90% of sequence in fragments shorter than    1855 bp
  95% of sequence in fragments shorter than    2396 bp

# HinfI.SspI (GANTC+AATATT) double digest, 11292 thousand fragments (8916054+2377267 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      88 bp
  10% of sequence in fragments shorter than     137 bp
  15% of sequence in fragments shorter than     177 bp
  20% of sequence in fragments shorter than     216 bp
  25% of sequence in fragments shorter than     253 bp
  30% of sequence in fragments shorter than     290 bp
  35% of sequence in fragments shorter than     324 bp
  40% of sequence in fragments shorter than     355 bp
  45% of sequence in fragments shorter than     393 bp
  50% of sequence in fragments shorter than     436 bp
  55% of sequence in fragments shorter than     480 bp
  60% of sequence in fragments shorter than     529 bp
  65% of sequence in fragments shorter than     584 bp
  70% of sequence in fragments shorter than     644 bp
  75% of sequence in fragments shorter than     709 bp
  80% of sequence in fragments shorter than     791 bp
  85% of sequence in fragments shorter than     896 bp
  90% of sequence in fragments shorter than    1038 bp
  95% of sequence in fragments shorter than    1277 bp

# HaeIII.SmlI (GGCC+CTYRAG) double digest, 11289 thousand fragments (8562230+2727867 sites), 263 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     134 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     229 bp
  25% of sequence in fragments shorter than     277 bp
  30% of sequence in fragments shorter than     324 bp
  35% of sequence in fragments shorter than     365 bp
  40% of sequence in fragments shorter than     417 bp
  45% of sequence in fragments shorter than     472 bp
  50% of sequence in fragments shorter than     528 bp
  55% of sequence in fragments shorter than     594 bp
  60% of sequence in fragments shorter than     667 bp
  65% of sequence in fragments shorter than     746 bp
  70% of sequence in fragments shorter than     841 bp
  75% of sequence in fragments shorter than     943 bp
  80% of sequence in fragments shorter than    1072 bp
  85% of sequence in fragments shorter than    1245 bp
  90% of sequence in fragments shorter than    1475 bp
  95% of sequence in fragments shorter than    1881 bp

# BccI.CviQI (CCATC+GTAC) double digest, 11285 thousand fragments (6170927+5115078 sites), 264 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     141 bp
  15% of sequence in fragments shorter than     181 bp
  20% of sequence in fragments shorter than     218 bp
  25% of sequence in fragments shorter than     254 bp
  30% of sequence in fragments shorter than     290 bp
  35% of sequence in fragments shorter than     329 bp
  40% of sequence in fragments shorter than     367 bp
  45% of sequence in fragments shorter than     409 bp
  50% of sequence in fragments shorter than     452 bp
  55% of sequence in fragments shorter than     499 bp
  60% of sequence in fragments shorter than     550 bp
  65% of sequence in fragments shorter than     607 bp
  70% of sequence in fragments shorter than     670 bp
  75% of sequence in fragments shorter than     743 bp
  80% of sequence in fragments shorter than     833 bp
  85% of sequence in fragments shorter than     946 bp
  90% of sequence in fragments shorter than    1104 bp
  95% of sequence in fragments shorter than    1378 bp

# AciI.Hin4II (CCGC+CCTTC) double digest, 11265 thousand fragments (4206130+7059915 sites), 264 bp average fragment length
   5% of sequence in fragments shorter than      88 bp
  10% of sequence in fragments shorter than     143 bp
  15% of sequence in fragments shorter than     191 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     337 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     494 bp
  50% of sequence in fragments shorter than     552 bp
  55% of sequence in fragments shorter than     618 bp
  60% of sequence in fragments shorter than     692 bp
  65% of sequence in fragments shorter than     773 bp
  70% of sequence in fragments shorter than     864 bp
  75% of sequence in fragments shorter than     973 bp
  80% of sequence in fragments shorter than    1103 bp
  85% of sequence in fragments shorter than    1275 bp
  90% of sequence in fragments shorter than    1512 bp
  95% of sequence in fragments shorter than    1923 bp

# HpaII.Hpy188I (CCGG+TCNGA) double digest, 11259 thousand fragments (2317722+8942153 sites), 264 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     136 bp
  15% of sequence in fragments shorter than     174 bp
  20% of sequence in fragments shorter than     212 bp
  25% of sequence in fragments shorter than     251 bp
  30% of sequence in fragments shorter than     292 bp
  35% of sequence in fragments shorter than     334 bp
  40% of sequence in fragments shorter than     371 bp
  45% of sequence in fragments shorter than     416 bp
  50% of sequence in fragments shorter than     463 bp
  55% of sequence in fragments shorter than     515 bp
  60% of sequence in fragments shorter than     570 bp
  65% of sequence in fragments shorter than     631 bp
  70% of sequence in fragments shorter than     702 bp
  75% of sequence in fragments shorter than     784 bp
  80% of sequence in fragments shorter than     880 bp
  85% of sequence in fragments shorter than    1003 bp
  90% of sequence in fragments shorter than    1171 bp
  95% of sequence in fragments shorter than    1440 bp

# HinfI.HpaII (GANTC+CCGG) double digest, 11233 thousand fragments (8916054+2317722 sites), 265 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     137 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     218 bp
  25% of sequence in fragments shorter than     259 bp
  30% of sequence in fragments shorter than     299 bp
  35% of sequence in fragments shorter than     338 bp
  40% of sequence in fragments shorter than     375 bp
  45% of sequence in fragments shorter than     420 bp
  50% of sequence in fragments shorter than     468 bp
  55% of sequence in fragments shorter than     521 bp
  60% of sequence in fragments shorter than     578 bp
  65% of sequence in fragments shorter than     643 bp
  70% of sequence in fragments shorter than     710 bp
  75% of sequence in fragments shorter than     792 bp
  80% of sequence in fragments shorter than     891 bp
  85% of sequence in fragments shorter than    1017 bp
  90% of sequence in fragments shorter than    1189 bp
  95% of sequence in fragments shorter than    1483 bp

# BspKT6I.Tsp45I (GATC+GTSAC) double digest, 11220 thousand fragments (7199434+4021761 sites), 265 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     142 bp
  15% of sequence in fragments shorter than     176 bp
  20% of sequence in fragments shorter than     206 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     285 bp
  35% of sequence in fragments shorter than     324 bp
  40% of sequence in fragments shorter than     360 bp
  45% of sequence in fragments shorter than     403 bp
  50% of sequence in fragments shorter than     447 bp
  55% of sequence in fragments shorter than     491 bp
  60% of sequence in fragments shorter than     543 bp
  65% of sequence in fragments shorter than     601 bp
  70% of sequence in fragments shorter than     666 bp
  75% of sequence in fragments shorter than     741 bp
  80% of sequence in fragments shorter than     833 bp
  85% of sequence in fragments shorter than     948 bp
  90% of sequence in fragments shorter than    1101 bp
  95% of sequence in fragments shorter than    1385 bp

# BspNCI.MlyI (CCAGA+GAGTC) double digest, 11213 thousand fragments (7282436+3931692 sites), 265 bp average fragment length
   5% of sequence in fragments shorter than      90 bp
  10% of sequence in fragments shorter than     139 bp
  15% of sequence in fragments shorter than     183 bp
  20% of sequence in fragments shorter than     224 bp
  25% of sequence in fragments shorter than     265 bp
  30% of sequence in fragments shorter than     305 bp
  35% of sequence in fragments shorter than     344 bp
  40% of sequence in fragments shorter than     386 bp
  45% of sequence in fragments shorter than     431 bp
  50% of sequence in fragments shorter than     478 bp
  55% of sequence in fragments shorter than     529 bp
  60% of sequence in fragments shorter than     584 bp
  65% of sequence in fragments shorter than     646 bp
  70% of sequence in fragments shorter than     716 bp
  75% of sequence in fragments shorter than     799 bp
  80% of sequence in fragments shorter than     902 bp
  85% of sequence in fragments shorter than    1036 bp
  90% of sequence in fragments shorter than    1209 bp
  95% of sequence in fragments shorter than    1526 bp

# FokI.HphI (GGATG+GGTGA) double digest, 11207 thousand fragments (5201116+6007336 sites), 265 bp average fragment length
   5% of sequence in fragments shorter than      92 bp
  10% of sequence in fragments shorter than     142 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     220 bp
  25% of sequence in fragments shorter than     259 bp
  30% of sequence in fragments shorter than     298 bp
  35% of sequence in fragments shorter than     338 bp
  40% of sequence in fragments shorter than     378 bp
  45% of sequence in fragments shorter than     422 bp
  50% of sequence in fragments shorter than     470 bp
  55% of sequence in fragments shorter than     521 bp
  60% of sequence in fragments shorter than     577 bp
  65% of sequence in fragments shorter than     638 bp
  70% of sequence in fragments shorter than     709 bp
  75% of sequence in fragments shorter than     793 bp
  80% of sequence in fragments shorter than     894 bp
  85% of sequence in fragments shorter than    1021 bp
  90% of sequence in fragments shorter than    1198 bp
  95% of sequence in fragments shorter than    1507 bp

# BthCI.TfiI (GCNGC+GAWTC) double digest, 11193 thousand fragments (6209929+4984362 sites), 266 bp average fragment length
   5% of sequence in fragments shorter than      90 bp
  10% of sequence in fragments shorter than     143 bp
  15% of sequence in fragments shorter than     187 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     271 bp
  30% of sequence in fragments shorter than     314 bp
  35% of sequence in fragments shorter than     353 bp
  40% of sequence in fragments shorter than     399 bp
  45% of sequence in fragments shorter than     449 bp
  50% of sequence in fragments shorter than     501 bp
  55% of sequence in fragments shorter than     557 bp
  60% of sequence in fragments shorter than     620 bp
  65% of sequence in fragments shorter than     686 bp
  70% of sequence in fragments shorter than     761 bp
  75% of sequence in fragments shorter than     848 bp
  80% of sequence in fragments shorter than     956 bp
  85% of sequence in fragments shorter than    1093 bp
  90% of sequence in fragments shorter than    1283 bp
  95% of sequence in fragments shorter than    1606 bp

# BstNI.PstI (CCWGG+CTGCAG) double digest, 11176 thousand fragments (9855646+1321469 sites), 266 bp average fragment length
   5% of sequence in fragments shorter than      74 bp
  10% of sequence in fragments shorter than     119 bp
  15% of sequence in fragments shorter than     165 bp
  20% of sequence in fragments shorter than     211 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     317 bp
  35% of sequence in fragments shorter than     375 bp
  40% of sequence in fragments shorter than     438 bp
  45% of sequence in fragments shorter than     507 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     662 bp
  60% of sequence in fragments shorter than     755 bp
  65% of sequence in fragments shorter than     864 bp
  70% of sequence in fragments shorter than     994 bp
  75% of sequence in fragments shorter than    1149 bp
  80% of sequence in fragments shorter than    1345 bp
  85% of sequence in fragments shorter than    1606 bp
  90% of sequence in fragments shorter than    1992 bp
  95% of sequence in fragments shorter than    2669 bp

# BccI.TfiI (CCATC+GAWTC) double digest, 11154 thousand fragments (6170927+4984362 sites), 267 bp average fragment length
   5% of sequence in fragments shorter than      90 bp
  10% of sequence in fragments shorter than     140 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     220 bp
  25% of sequence in fragments shorter than     259 bp
  30% of sequence in fragments shorter than     297 bp
  35% of sequence in fragments shorter than     335 bp
  40% of sequence in fragments shorter than     370 bp
  45% of sequence in fragments shorter than     414 bp
  50% of sequence in fragments shorter than     459 bp
  55% of sequence in fragments shorter than     507 bp
  60% of sequence in fragments shorter than     561 bp
  65% of sequence in fragments shorter than     620 bp
  70% of sequence in fragments shorter than     686 bp
  75% of sequence in fragments shorter than     761 bp
  80% of sequence in fragments shorter than     853 bp
  85% of sequence in fragments shorter than     970 bp
  90% of sequence in fragments shorter than    1130 bp
  95% of sequence in fragments shorter than    1404 bp

# SspI.TspRI (AATATT+CASTG) double digest, 11142 thousand fragments (2377267+8765240 sites), 267 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     144 bp
  15% of sequence in fragments shorter than     187 bp
  20% of sequence in fragments shorter than     220 bp
  25% of sequence in fragments shorter than     256 bp
  30% of sequence in fragments shorter than     293 bp
  35% of sequence in fragments shorter than     330 bp
  40% of sequence in fragments shorter than     367 bp
  45% of sequence in fragments shorter than     408 bp
  50% of sequence in fragments shorter than     451 bp
  55% of sequence in fragments shorter than     498 bp
  60% of sequence in fragments shorter than     549 bp
  65% of sequence in fragments shorter than     607 bp
  70% of sequence in fragments shorter than     671 bp
  75% of sequence in fragments shorter than     745 bp
  80% of sequence in fragments shorter than     836 bp
  85% of sequence in fragments shorter than     949 bp
  90% of sequence in fragments shorter than    1105 bp
  95% of sequence in fragments shorter than    1385 bp

# BspKT6I.MlyI (GATC+GAGTC) double digest, 11130 thousand fragments (7199434+3931692 sites), 267 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     142 bp
  15% of sequence in fragments shorter than     177 bp
  20% of sequence in fragments shorter than     209 bp
  25% of sequence in fragments shorter than     246 bp
  30% of sequence in fragments shorter than     286 bp
  35% of sequence in fragments shorter than     327 bp
  40% of sequence in fragments shorter than     367 bp
  45% of sequence in fragments shorter than     411 bp
  50% of sequence in fragments shorter than     457 bp
  55% of sequence in fragments shorter than     505 bp
  60% of sequence in fragments shorter than     560 bp
  65% of sequence in fragments shorter than     620 bp
  70% of sequence in fragments shorter than     689 bp
  75% of sequence in fragments shorter than     769 bp
  80% of sequence in fragments shorter than     867 bp
  85% of sequence in fragments shorter than     995 bp
  90% of sequence in fragments shorter than    1164 bp
  95% of sequence in fragments shorter than    1472 bp

# CviQI.HphI (GTAC+GGTGA) double digest, 11121 thousand fragments (5115078+6007336 sites), 267 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     142 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     219 bp
  25% of sequence in fragments shorter than     257 bp
  30% of sequence in fragments shorter than     293 bp
  35% of sequence in fragments shorter than     331 bp
  40% of sequence in fragments shorter than     370 bp
  45% of sequence in fragments shorter than     411 bp
  50% of sequence in fragments shorter than     454 bp
  55% of sequence in fragments shorter than     500 bp
  60% of sequence in fragments shorter than     549 bp
  65% of sequence in fragments shorter than     605 bp
  70% of sequence in fragments shorter than     667 bp
  75% of sequence in fragments shorter than     738 bp
  80% of sequence in fragments shorter than     824 bp
  85% of sequence in fragments shorter than     933 bp
  90% of sequence in fragments shorter than    1090 bp
  95% of sequence in fragments shorter than    1356 bp

# BscGI.BstNI (CCCGT+CCWGG) double digest, 11086 thousand fragments (1231070+9855646 sites), 268 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     120 bp
  15% of sequence in fragments shorter than     166 bp
  20% of sequence in fragments shorter than     213 bp
  25% of sequence in fragments shorter than     267 bp
  30% of sequence in fragments shorter than     325 bp
  35% of sequence in fragments shorter than     373 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     508 bp
  50% of sequence in fragments shorter than     584 bp
  55% of sequence in fragments shorter than     666 bp
  60% of sequence in fragments shorter than     757 bp
  65% of sequence in fragments shorter than     867 bp
  70% of sequence in fragments shorter than     997 bp
  75% of sequence in fragments shorter than    1149 bp
  80% of sequence in fragments shorter than    1339 bp
  85% of sequence in fragments shorter than    1591 bp
  90% of sequence in fragments shorter than    1945 bp
  95% of sequence in fragments shorter than    2547 bp

# HpaII.TspRI (CCGG+CASTG) double digest, 11082 thousand fragments (2317722+8765240 sites), 268 bp average fragment length
   5% of sequence in fragments shorter than      88 bp
  10% of sequence in fragments shorter than     140 bp
  15% of sequence in fragments shorter than     189 bp
  20% of sequence in fragments shorter than     224 bp
  25% of sequence in fragments shorter than     266 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     357 bp
  40% of sequence in fragments shorter than     406 bp
  45% of sequence in fragments shorter than     457 bp
  50% of sequence in fragments shorter than     513 bp
  55% of sequence in fragments shorter than     572 bp
  60% of sequence in fragments shorter than     639 bp
  65% of sequence in fragments shorter than     711 bp
  70% of sequence in fragments shorter than     797 bp
  75% of sequence in fragments shorter than     896 bp
  80% of sequence in fragments shorter than    1012 bp
  85% of sequence in fragments shorter than    1165 bp
  90% of sequence in fragments shorter than    1381 bp
  95% of sequence in fragments shorter than    1772 bp

# Hin4II.Tsp45I (CCTTC+GTSAC) double digest, 11081 thousand fragments (7059915+4021761 sites), 268 bp average fragment length
   5% of sequence in fragments shorter than      91 bp
  10% of sequence in fragments shorter than     142 bp
  15% of sequence in fragments shorter than     187 bp
  20% of sequence in fragments shorter than     229 bp
  25% of sequence in fragments shorter than     270 bp
  30% of sequence in fragments shorter than     313 bp
  35% of sequence in fragments shorter than     351 bp
  40% of sequence in fragments shorter than     394 bp
  45% of sequence in fragments shorter than     440 bp
  50% of sequence in fragments shorter than     491 bp
  55% of sequence in fragments shorter than     542 bp
  60% of sequence in fragments shorter than     601 bp
  65% of sequence in fragments shorter than     667 bp
  70% of sequence in fragments shorter than     741 bp
  75% of sequence in fragments shorter than     829 bp
  80% of sequence in fragments shorter than     932 bp
  85% of sequence in fragments shorter than    1064 bp
  90% of sequence in fragments shorter than    1255 bp
  95% of sequence in fragments shorter than    1579 bp

# BbvI.BsrI (GCAGC+ACTGG) double digest, 11030 thousand fragments (5290051+5741310 sites), 270 bp average fragment length
   5% of sequence in fragments shorter than      88 bp
  10% of sequence in fragments shorter than     140 bp
  15% of sequence in fragments shorter than     185 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     274 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     367 bp
  40% of sequence in fragments shorter than     418 bp
  45% of sequence in fragments shorter than     469 bp
  50% of sequence in fragments shorter than     526 bp
  55% of sequence in fragments shorter than     588 bp
  60% of sequence in fragments shorter than     656 bp
  65% of sequence in fragments shorter than     722 bp
  70% of sequence in fragments shorter than     806 bp
  75% of sequence in fragments shorter than     897 bp
  80% of sequence in fragments shorter than    1017 bp
  85% of sequence in fragments shorter than    1165 bp
  90% of sequence in fragments shorter than    1378 bp
  95% of sequence in fragments shorter than    1747 bp

# HphI.TfiI (GGTGA+GAWTC) double digest, 10991 thousand fragments (6007336+4984362 sites), 271 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     139 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     220 bp
  25% of sequence in fragments shorter than     260 bp
  30% of sequence in fragments shorter than     299 bp
  35% of sequence in fragments shorter than     337 bp
  40% of sequence in fragments shorter than     373 bp
  45% of sequence in fragments shorter than     416 bp
  50% of sequence in fragments shorter than     462 bp
  55% of sequence in fragments shorter than     510 bp
  60% of sequence in fragments shorter than     563 bp
  65% of sequence in fragments shorter than     621 bp
  70% of sequence in fragments shorter than     687 bp
  75% of sequence in fragments shorter than     762 bp
  80% of sequence in fragments shorter than     854 bp
  85% of sequence in fragments shorter than     972 bp
  90% of sequence in fragments shorter than    1133 bp
  95% of sequence in fragments shorter than    1404 bp

# Hin4II.MlyI (CCTTC+GAGTC) double digest, 10991 thousand fragments (7059915+3931692 sites), 271 bp average fragment length
   5% of sequence in fragments shorter than      92 bp
  10% of sequence in fragments shorter than     143 bp
  15% of sequence in fragments shorter than     190 bp
  20% of sequence in fragments shorter than     232 bp
  25% of sequence in fragments shorter than     274 bp
  30% of sequence in fragments shorter than     315 bp
  35% of sequence in fragments shorter than     354 bp
  40% of sequence in fragments shorter than     397 bp
  45% of sequence in fragments shorter than     442 bp
  50% of sequence in fragments shorter than     493 bp
  55% of sequence in fragments shorter than     545 bp
  60% of sequence in fragments shorter than     604 bp
  65% of sequence in fragments shorter than     669 bp
  70% of sequence in fragments shorter than     744 bp
  75% of sequence in fragments shorter than     834 bp
  80% of sequence in fragments shorter than     942 bp
  85% of sequence in fragments shorter than    1074 bp
  90% of sequence in fragments shorter than    1269 bp
  95% of sequence in fragments shorter than    1595 bp

# AccI.BstNI (GTMKAC+CCWGG) double digest, 10956 thousand fragments (1101063+9855646 sites), 271 bp average fragment length
   5% of sequence in fragments shorter than      79 bp
  10% of sequence in fragments shorter than     122 bp
  15% of sequence in fragments shorter than     168 bp
  20% of sequence in fragments shorter than     213 bp
  25% of sequence in fragments shorter than     262 bp
  30% of sequence in fragments shorter than     314 bp
  35% of sequence in fragments shorter than     367 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     487 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     627 bp
  60% of sequence in fragments shorter than     708 bp
  65% of sequence in fragments shorter than     802 bp
  70% of sequence in fragments shorter than     913 bp
  75% of sequence in fragments shorter than    1044 bp
  80% of sequence in fragments shorter than    1208 bp
  85% of sequence in fragments shorter than    1415 bp
  90% of sequence in fragments shorter than    1730 bp
  95% of sequence in fragments shorter than    2256 bp

# HhaI.MboII (GCGC+GAAGA) double digest, 10955 thousand fragments (1756502+9199496 sites), 272 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     186 bp
  20% of sequence in fragments shorter than     229 bp
  25% of sequence in fragments shorter than     271 bp
  30% of sequence in fragments shorter than     313 bp
  35% of sequence in fragments shorter than     354 bp
  40% of sequence in fragments shorter than     400 bp
  45% of sequence in fragments shorter than     446 bp
  50% of sequence in fragments shorter than     496 bp
  55% of sequence in fragments shorter than     549 bp
  60% of sequence in fragments shorter than     608 bp
  65% of sequence in fragments shorter than     672 bp
  70% of sequence in fragments shorter than     744 bp
  75% of sequence in fragments shorter than     828 bp
  80% of sequence in fragments shorter than     926 bp
  85% of sequence in fragments shorter than    1045 bp
  90% of sequence in fragments shorter than    1216 bp
  95% of sequence in fragments shorter than    1503 bp

# BsrI.FokI (ACTGG+GGATG) double digest, 10941 thousand fragments (5741310+5201116 sites), 272 bp average fragment length
   5% of sequence in fragments shorter than      92 bp
  10% of sequence in fragments shorter than     141 bp
  15% of sequence in fragments shorter than     185 bp
  20% of sequence in fragments shorter than     227 bp
  25% of sequence in fragments shorter than     268 bp
  30% of sequence in fragments shorter than     311 bp
  35% of sequence in fragments shorter than     353 bp
  40% of sequence in fragments shorter than     398 bp
  45% of sequence in fragments shorter than     445 bp
  50% of sequence in fragments shorter than     493 bp
  55% of sequence in fragments shorter than     547 bp
  60% of sequence in fragments shorter than     605 bp
  65% of sequence in fragments shorter than     669 bp
  70% of sequence in fragments shorter than     740 bp
  75% of sequence in fragments shorter than     824 bp
  80% of sequence in fragments shorter than     928 bp
  85% of sequence in fragments shorter than    1061 bp
  90% of sequence in fragments shorter than    1248 bp
  95% of sequence in fragments shorter than    1571 bp

# BstNI.PpuMI (CCWGG+RGGWCCY) double digest, 10940 thousand fragments (9855646+1085138 sites), 272 bp average fragment length
   5% of sequence in fragments shorter than      77 bp
  10% of sequence in fragments shorter than     122 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     218 bp
  25% of sequence in fragments shorter than     272 bp
  30% of sequence in fragments shorter than     327 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     521 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     681 bp
  60% of sequence in fragments shorter than     778 bp
  65% of sequence in fragments shorter than     893 bp
  70% of sequence in fragments shorter than    1026 bp
  75% of sequence in fragments shorter than    1189 bp
  80% of sequence in fragments shorter than    1392 bp
  85% of sequence in fragments shorter than    1664 bp
  90% of sequence in fragments shorter than    2066 bp
  95% of sequence in fragments shorter than    2857 bp

# BstNI.PvuII (CCWGG+CAGCTG) double digest, 10939 thousand fragments (9855646+1084260 sites), 272 bp average fragment length
   5% of sequence in fragments shorter than      75 bp
  10% of sequence in fragments shorter than     120 bp
  15% of sequence in fragments shorter than     167 bp
  20% of sequence in fragments shorter than     213 bp
  25% of sequence in fragments shorter than     265 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     380 bp
  40% of sequence in fragments shorter than     443 bp
  45% of sequence in fragments shorter than     513 bp
  50% of sequence in fragments shorter than     591 bp
  55% of sequence in fragments shorter than     671 bp
  60% of sequence in fragments shorter than     766 bp
  65% of sequence in fragments shorter than     878 bp
  70% of sequence in fragments shorter than    1012 bp
  75% of sequence in fragments shorter than    1172 bp
  80% of sequence in fragments shorter than    1374 bp
  85% of sequence in fragments shorter than    1647 bp
  90% of sequence in fragments shorter than    2054 bp
  95% of sequence in fragments shorter than    2777 bp

# HaeIII.SspI (GGCC+AATATT) double digest, 10939 thousand fragments (8562230+2377267 sites), 272 bp average fragment length
   5% of sequence in fragments shorter than      86 bp
  10% of sequence in fragments shorter than     141 bp
  15% of sequence in fragments shorter than     188 bp
  20% of sequence in fragments shorter than     235 bp
  25% of sequence in fragments shorter than     279 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     361 bp
  40% of sequence in fragments shorter than     409 bp
  45% of sequence in fragments shorter than     456 bp
  50% of sequence in fragments shorter than     509 bp
  55% of sequence in fragments shorter than     563 bp
  60% of sequence in fragments shorter than     623 bp
  65% of sequence in fragments shorter than     690 bp
  70% of sequence in fragments shorter than     766 bp
  75% of sequence in fragments shorter than     853 bp
  80% of sequence in fragments shorter than     953 bp
  85% of sequence in fragments shorter than    1086 bp
  90% of sequence in fragments shorter than    1267 bp
  95% of sequence in fragments shorter than    1569 bp

# BscAI.BspNCI (GCATC+CCAGA) double digest, 10934 thousand fragments (3652926+7282436 sites), 272 bp average fragment length
   5% of sequence in fragments shorter than      91 bp
  10% of sequence in fragments shorter than     140 bp
  15% of sequence in fragments shorter than     184 bp
  20% of sequence in fragments shorter than     227 bp
  25% of sequence in fragments shorter than     268 bp
  30% of sequence in fragments shorter than     311 bp
  35% of sequence in fragments shorter than     354 bp
  40% of sequence in fragments shorter than     400 bp
  45% of sequence in fragments shorter than     447 bp
  50% of sequence in fragments shorter than     498 bp
  55% of sequence in fragments shorter than     552 bp
  60% of sequence in fragments shorter than     607 bp
  65% of sequence in fragments shorter than     674 bp
  70% of sequence in fragments shorter than     748 bp
  75% of sequence in fragments shorter than     837 bp
  80% of sequence in fragments shorter than     941 bp
  85% of sequence in fragments shorter than    1076 bp
  90% of sequence in fragments shorter than    1267 bp
  95% of sequence in fragments shorter than    1604 bp

# BfiI.MboII (ACTGGG+GAAGA) double digest, 10905 thousand fragments (1706594+9199496 sites), 273 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     146 bp
  15% of sequence in fragments shorter than     184 bp
  20% of sequence in fragments shorter than     228 bp
  25% of sequence in fragments shorter than     270 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     351 bp
  40% of sequence in fragments shorter than     396 bp
  45% of sequence in fragments shorter than     442 bp
  50% of sequence in fragments shorter than     492 bp
  55% of sequence in fragments shorter than     545 bp
  60% of sequence in fragments shorter than     604 bp
  65% of sequence in fragments shorter than     668 bp
  70% of sequence in fragments shorter than     741 bp
  75% of sequence in fragments shorter than     825 bp
  80% of sequence in fragments shorter than     927 bp
  85% of sequence in fragments shorter than    1051 bp
  90% of sequence in fragments shorter than    1229 bp
  95% of sequence in fragments shorter than    1534 bp

# HaeIII.HpaII (GGCC+CCGG) double digest, 10879 thousand fragments (8562230+2317722 sites), 273 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     140 bp
  15% of sequence in fragments shorter than     197 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     370 bp
  35% of sequence in fragments shorter than     434 bp
  40% of sequence in fragments shorter than     503 bp
  45% of sequence in fragments shorter than     575 bp
  50% of sequence in fragments shorter than     655 bp
  55% of sequence in fragments shorter than     742 bp
  60% of sequence in fragments shorter than     842 bp
  65% of sequence in fragments shorter than     947 bp
  70% of sequence in fragments shorter than    1072 bp
  75% of sequence in fragments shorter than    1223 bp
  80% of sequence in fragments shorter than    1398 bp
  85% of sequence in fragments shorter than    1638 bp
  90% of sequence in fragments shorter than    1970 bp
  95% of sequence in fragments shorter than    2563 bp

# AvaI.BstNI (CYCGRG+CCWGG) double digest, 10860 thousand fragments (1005714+9855646 sites), 274 bp average fragment length
   5% of sequence in fragments shorter than      71 bp
  10% of sequence in fragments shorter than     119 bp
  15% of sequence in fragments shorter than     168 bp
  20% of sequence in fragments shorter than     221 bp
  25% of sequence in fragments shorter than     278 bp
  30% of sequence in fragments shorter than     336 bp
  35% of sequence in fragments shorter than     400 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     546 bp
  50% of sequence in fragments shorter than     629 bp
  55% of sequence in fragments shorter than     719 bp
  60% of sequence in fragments shorter than     824 bp
  65% of sequence in fragments shorter than     947 bp
  70% of sequence in fragments shorter than    1092 bp
  75% of sequence in fragments shorter than    1266 bp
  80% of sequence in fragments shorter than    1488 bp
  85% of sequence in fragments shorter than    1789 bp
  90% of sequence in fragments shorter than    2219 bp
  95% of sequence in fragments shorter than    3052 bp

# BsrI.CviQI (ACTGG+GTAC) double digest, 10855 thousand fragments (5741310+5115078 sites), 274 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     189 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     271 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     352 bp
  40% of sequence in fragments shorter than     395 bp
  45% of sequence in fragments shorter than     441 bp
  50% of sequence in fragments shorter than     486 bp
  55% of sequence in fragments shorter than     535 bp
  60% of sequence in fragments shorter than     590 bp
  65% of sequence in fragments shorter than     651 bp
  70% of sequence in fragments shorter than     714 bp
  75% of sequence in fragments shorter than     793 bp
  80% of sequence in fragments shorter than     888 bp
  85% of sequence in fragments shorter than    1012 bp
  90% of sequence in fragments shorter than    1180 bp
  95% of sequence in fragments shorter than    1467 bp

# BscAI.BspKT6I (GCATC+GATC) double digest, 10851 thousand fragments (3652926+7199434 sites), 274 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     145 bp
  15% of sequence in fragments shorter than     178 bp
  20% of sequence in fragments shorter than     214 bp
  25% of sequence in fragments shorter than     255 bp
  30% of sequence in fragments shorter than     294 bp
  35% of sequence in fragments shorter than     332 bp
  40% of sequence in fragments shorter than     371 bp
  45% of sequence in fragments shorter than     414 bp
  50% of sequence in fragments shorter than     458 bp
  55% of sequence in fragments shorter than     505 bp
  60% of sequence in fragments shorter than     558 bp
  65% of sequence in fragments shorter than     616 bp
  70% of sequence in fragments shorter than     681 bp
  75% of sequence in fragments shorter than     758 bp
  80% of sequence in fragments shorter than     851 bp
  85% of sequence in fragments shorter than     970 bp
  90% of sequence in fragments shorter than    1135 bp
  95% of sequence in fragments shorter than    1429 bp

# MboII.PsiI (GAAGA+TTATAA) double digest, 10846 thousand fragments (9199496+1647912 sites), 274 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     146 bp
  15% of sequence in fragments shorter than     184 bp
  20% of sequence in fragments shorter than     228 bp
  25% of sequence in fragments shorter than     270 bp
  30% of sequence in fragments shorter than     314 bp
  35% of sequence in fragments shorter than     353 bp
  40% of sequence in fragments shorter than     401 bp
  45% of sequence in fragments shorter than     447 bp
  50% of sequence in fragments shorter than     498 bp
  55% of sequence in fragments shorter than     551 bp
  60% of sequence in fragments shorter than     609 bp
  65% of sequence in fragments shorter than     676 bp
  70% of sequence in fragments shorter than     750 bp
  75% of sequence in fragments shorter than     838 bp
  80% of sequence in fragments shorter than     943 bp
  85% of sequence in fragments shorter than    1072 bp
  90% of sequence in fragments shorter than    1260 bp
  95% of sequence in fragments shorter than    1590 bp

# AciI.BsmAI (CCGC+GTCTC) double digest, 10837 thousand fragments (4206130+6631585 sites), 274 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     141 bp
  15% of sequence in fragments shorter than     192 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     364 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     490 bp
  45% of sequence in fragments shorter than     557 bp
  50% of sequence in fragments shorter than     629 bp
  55% of sequence in fragments shorter than     708 bp
  60% of sequence in fragments shorter than     799 bp
  65% of sequence in fragments shorter than     897 bp
  70% of sequence in fragments shorter than    1012 bp
  75% of sequence in fragments shorter than    1143 bp
  80% of sequence in fragments shorter than    1308 bp
  85% of sequence in fragments shorter than    1509 bp
  90% of sequence in fragments shorter than    1796 bp
  95% of sequence in fragments shorter than    2310 bp

# BspHI.BstNI (TCATGA+CCWGG) double digest, 10833 thousand fragments (978290+9855646 sites), 275 bp average fragment length
   5% of sequence in fragments shorter than      79 bp
  10% of sequence in fragments shorter than     123 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     216 bp
  25% of sequence in fragments shorter than     266 bp
  30% of sequence in fragments shorter than     319 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     497 bp
  50% of sequence in fragments shorter than     567 bp
  55% of sequence in fragments shorter than     644 bp
  60% of sequence in fragments shorter than     727 bp
  65% of sequence in fragments shorter than     825 bp
  70% of sequence in fragments shorter than     942 bp
  75% of sequence in fragments shorter than    1080 bp
  80% of sequence in fragments shorter than    1253 bp
  85% of sequence in fragments shorter than    1484 bp
  90% of sequence in fragments shorter than    1831 bp
  95% of sequence in fragments shorter than    2472 bp

# BspNCI.SfeI (CCAGA+CTRYAG) double digest, 10812 thousand fragments (7282436+3531009 sites), 275 bp average fragment length
   5% of sequence in fragments shorter than      91 bp
  10% of sequence in fragments shorter than     142 bp
  15% of sequence in fragments shorter than     185 bp
  20% of sequence in fragments shorter than     227 bp
  25% of sequence in fragments shorter than     268 bp
  30% of sequence in fragments shorter than     309 bp
  35% of sequence in fragments shorter than     348 bp
  40% of sequence in fragments shorter than     392 bp
  45% of sequence in fragments shorter than     438 bp
  50% of sequence in fragments shorter than     485 bp
  55% of sequence in fragments shorter than     536 bp
  60% of sequence in fragments shorter than     590 bp
  65% of sequence in fragments shorter than     648 bp
  70% of sequence in fragments shorter than     717 bp
  75% of sequence in fragments shorter than     798 bp
  80% of sequence in fragments shorter than     898 bp
  85% of sequence in fragments shorter than    1026 bp
  90% of sequence in fragments shorter than    1199 bp
  95% of sequence in fragments shorter than    1504 bp

# EsaBC3I.MboII (TCGA+GAAGA) double digest, 10802 thousand fragments (1603346+9199496 sites), 275 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     189 bp
  20% of sequence in fragments shorter than     232 bp
  25% of sequence in fragments shorter than     274 bp
  30% of sequence in fragments shorter than     316 bp
  35% of sequence in fragments shorter than     355 bp
  40% of sequence in fragments shorter than     400 bp
  45% of sequence in fragments shorter than     444 bp
  50% of sequence in fragments shorter than     493 bp
  55% of sequence in fragments shorter than     545 bp
  60% of sequence in fragments shorter than     603 bp
  65% of sequence in fragments shorter than     666 bp
  70% of sequence in fragments shorter than     737 bp
  75% of sequence in fragments shorter than     819 bp
  80% of sequence in fragments shorter than     915 bp
  85% of sequence in fragments shorter than    1033 bp
  90% of sequence in fragments shorter than    1201 bp
  95% of sequence in fragments shorter than    1485 bp

# AvaIII.BstNI (ATGCAT+CCWGG) double digest, 10788 thousand fragments (933357+9855646 sites), 276 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     169 bp
  20% of sequence in fragments shorter than     216 bp
  25% of sequence in fragments shorter than     267 bp
  30% of sequence in fragments shorter than     319 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     431 bp
  45% of sequence in fragments shorter than     495 bp
  50% of sequence in fragments shorter than     564 bp
  55% of sequence in fragments shorter than     639 bp
  60% of sequence in fragments shorter than     721 bp
  65% of sequence in fragments shorter than     818 bp
  70% of sequence in fragments shorter than     934 bp
  75% of sequence in fragments shorter than    1071 bp
  80% of sequence in fragments shorter than    1244 bp
  85% of sequence in fragments shorter than    1474 bp
  90% of sequence in fragments shorter than    1827 bp
  95% of sequence in fragments shorter than    2449 bp

# BstNI.StuI (CCWGG+AGGCCT) double digest, 10780 thousand fragments (9855646+925729 sites), 276 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     221 bp
  25% of sequence in fragments shorter than     275 bp
  30% of sequence in fragments shorter than     331 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     520 bp
  50% of sequence in fragments shorter than     598 bp
  55% of sequence in fragments shorter than     681 bp
  60% of sequence in fragments shorter than     764 bp
  65% of sequence in fragments shorter than     872 bp
  70% of sequence in fragments shorter than     999 bp
  75% of sequence in fragments shorter than    1150 bp
  80% of sequence in fragments shorter than    1338 bp
  85% of sequence in fragments shorter than    1579 bp
  90% of sequence in fragments shorter than    1930 bp
  95% of sequence in fragments shorter than    2519 bp

# BstNI.NdeI (CCWGG+CATATG) double digest, 10758 thousand fragments (9855646+903360 sites), 276 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     218 bp
  25% of sequence in fragments shorter than     269 bp
  30% of sequence in fragments shorter than     319 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     432 bp
  45% of sequence in fragments shorter than     494 bp
  50% of sequence in fragments shorter than     560 bp
  55% of sequence in fragments shorter than     634 bp
  60% of sequence in fragments shorter than     715 bp
  65% of sequence in fragments shorter than     806 bp
  70% of sequence in fragments shorter than     915 bp
  75% of sequence in fragments shorter than    1045 bp
  80% of sequence in fragments shorter than    1195 bp
  85% of sequence in fragments shorter than    1404 bp
  90% of sequence in fragments shorter than    1718 bp
  95% of sequence in fragments shorter than    2353 bp

# HgiCI.MboII (GGYRCC+GAAGA) double digest, 10749 thousand fragments (1550877+9199496 sites), 277 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     187 bp
  20% of sequence in fragments shorter than     229 bp
  25% of sequence in fragments shorter than     270 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     350 bp
  40% of sequence in fragments shorter than     395 bp
  45% of sequence in fragments shorter than     442 bp
  50% of sequence in fragments shorter than     492 bp
  55% of sequence in fragments shorter than     544 bp
  60% of sequence in fragments shorter than     602 bp
  65% of sequence in fragments shorter than     665 bp
  70% of sequence in fragments shorter than     738 bp
  75% of sequence in fragments shorter than     821 bp
  80% of sequence in fragments shorter than     915 bp
  85% of sequence in fragments shorter than    1037 bp
  90% of sequence in fragments shorter than    1208 bp
  95% of sequence in fragments shorter than    1499 bp

# BsmI.MboII (GAATGC+GAAGA) double digest, 10748 thousand fragments (1549350+9199496 sites), 277 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     146 bp
  15% of sequence in fragments shorter than     187 bp
  20% of sequence in fragments shorter than     233 bp
  25% of sequence in fragments shorter than     278 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     369 bp
  40% of sequence in fragments shorter than     419 bp
  45% of sequence in fragments shorter than     468 bp
  50% of sequence in fragments shorter than     521 bp
  55% of sequence in fragments shorter than     578 bp
  60% of sequence in fragments shorter than     639 bp
  65% of sequence in fragments shorter than     708 bp
  70% of sequence in fragments shorter than     786 bp
  75% of sequence in fragments shorter than     876 bp
  80% of sequence in fragments shorter than     984 bp
  85% of sequence in fragments shorter than    1122 bp
  90% of sequence in fragments shorter than    1316 bp
  95% of sequence in fragments shorter than    1645 bp

# BspKT6I.SfeI (GATC+CTRYAG) double digest, 10729 thousand fragments (7199434+3531009 sites), 277 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     143 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     221 bp
  25% of sequence in fragments shorter than     264 bp
  30% of sequence in fragments shorter than     306 bp
  35% of sequence in fragments shorter than     346 bp
  40% of sequence in fragments shorter than     389 bp
  45% of sequence in fragments shorter than     433 bp
  50% of sequence in fragments shorter than     480 bp
  55% of sequence in fragments shorter than     531 bp
  60% of sequence in fragments shorter than     587 bp
  65% of sequence in fragments shorter than     650 bp
  70% of sequence in fragments shorter than     717 bp
  75% of sequence in fragments shorter than     800 bp
  80% of sequence in fragments shorter than     900 bp
  85% of sequence in fragments shorter than    1020 bp
  90% of sequence in fragments shorter than    1195 bp
  95% of sequence in fragments shorter than    1492 bp

# BsrI.TfiI (ACTGG+GAWTC) double digest, 10725 thousand fragments (5741310+4984362 sites), 277 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     146 bp
  15% of sequence in fragments shorter than     186 bp
  20% of sequence in fragments shorter than     226 bp
  25% of sequence in fragments shorter than     265 bp
  30% of sequence in fragments shorter than     305 bp
  35% of sequence in fragments shorter than     343 bp
  40% of sequence in fragments shorter than     378 bp
  45% of sequence in fragments shorter than     423 bp
  50% of sequence in fragments shorter than     470 bp
  55% of sequence in fragments shorter than     519 bp
  60% of sequence in fragments shorter than     573 bp
  65% of sequence in fragments shorter than     633 bp
  70% of sequence in fragments shorter than     699 bp
  75% of sequence in fragments shorter than     776 bp
  80% of sequence in fragments shorter than     869 bp
  85% of sequence in fragments shorter than     987 bp
  90% of sequence in fragments shorter than    1150 bp
  95% of sequence in fragments shorter than    1422 bp

# BstNI.HindIII (CCWGG+AAGCTT) double digest, 10715 thousand fragments (9855646+860365 sites), 278 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     123 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     218 bp
  25% of sequence in fragments shorter than     269 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     378 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     576 bp
  55% of sequence in fragments shorter than     654 bp
  60% of sequence in fragments shorter than     739 bp
  65% of sequence in fragments shorter than     841 bp
  70% of sequence in fragments shorter than     961 bp
  75% of sequence in fragments shorter than    1103 bp
  80% of sequence in fragments shorter than    1283 bp
  85% of sequence in fragments shorter than    1519 bp
  90% of sequence in fragments shorter than    1873 bp
  95% of sequence in fragments shorter than    2516 bp

# BscAI.Hin4II (GCATC+CCTTC) double digest, 10712 thousand fragments (3652926+7059915 sites), 278 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     193 bp
  20% of sequence in fragments shorter than     237 bp
  25% of sequence in fragments shorter than     279 bp
  30% of sequence in fragments shorter than     324 bp
  35% of sequence in fragments shorter than     367 bp
  40% of sequence in fragments shorter than     416 bp
  45% of sequence in fragments shorter than     468 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     578 bp
  60% of sequence in fragments shorter than     642 bp
  65% of sequence in fragments shorter than     713 bp
  70% of sequence in fragments shorter than     794 bp
  75% of sequence in fragments shorter than     886 bp
  80% of sequence in fragments shorter than    1002 bp
  85% of sequence in fragments shorter than    1142 bp
  90% of sequence in fragments shorter than    1345 bp
  95% of sequence in fragments shorter than    1690 bp

# BstNI.XbaI (CCWGG+TCTAGA) double digest, 10706 thousand fragments (9855646+850998 sites), 278 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     219 bp
  25% of sequence in fragments shorter than     271 bp
  30% of sequence in fragments shorter than     326 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     440 bp
  45% of sequence in fragments shorter than     506 bp
  50% of sequence in fragments shorter than     578 bp
  55% of sequence in fragments shorter than     655 bp
  60% of sequence in fragments shorter than     737 bp
  65% of sequence in fragments shorter than     836 bp
  70% of sequence in fragments shorter than     950 bp
  75% of sequence in fragments shorter than    1083 bp
  80% of sequence in fragments shorter than    1251 bp
  85% of sequence in fragments shorter than    1454 bp
  90% of sequence in fragments shorter than    1756 bp
  95% of sequence in fragments shorter than    2317 bp

# BstNI.EcoRI (CCWGG+GAATTC) double digest, 10702 thousand fragments (9855646+847401 sites), 278 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     220 bp
  25% of sequence in fragments shorter than     272 bp
  30% of sequence in fragments shorter than     327 bp
  35% of sequence in fragments shorter than     380 bp
  40% of sequence in fragments shorter than     440 bp
  45% of sequence in fragments shorter than     507 bp
  50% of sequence in fragments shorter than     579 bp
  55% of sequence in fragments shorter than     657 bp
  60% of sequence in fragments shorter than     739 bp
  65% of sequence in fragments shorter than     841 bp
  70% of sequence in fragments shorter than     961 bp
  75% of sequence in fragments shorter than    1103 bp
  80% of sequence in fragments shorter than    1280 bp
  85% of sequence in fragments shorter than    1511 bp
  90% of sequence in fragments shorter than    1853 bp
  95% of sequence in fragments shorter than    2443 bp

# HhaI.Hpy188I (GCGC+TCNGA) double digest, 10698 thousand fragments (1756502+8942153 sites), 278 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     144 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     226 bp
  25% of sequence in fragments shorter than     268 bp
  30% of sequence in fragments shorter than     311 bp
  35% of sequence in fragments shorter than     351 bp
  40% of sequence in fragments shorter than     395 bp
  45% of sequence in fragments shorter than     442 bp
  50% of sequence in fragments shorter than     491 bp
  55% of sequence in fragments shorter than     545 bp
  60% of sequence in fragments shorter than     603 bp
  65% of sequence in fragments shorter than     668 bp
  70% of sequence in fragments shorter than     741 bp
  75% of sequence in fragments shorter than     826 bp
  80% of sequence in fragments shorter than     928 bp
  85% of sequence in fragments shorter than    1055 bp
  90% of sequence in fragments shorter than    1228 bp
  95% of sequence in fragments shorter than    1509 bp

# AflIII.MboII (ACRYGT+GAAGA) double digest, 10684 thousand fragments (1485394+9199496 sites), 278 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     189 bp
  20% of sequence in fragments shorter than     233 bp
  25% of sequence in fragments shorter than     277 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     360 bp
  40% of sequence in fragments shorter than     406 bp
  45% of sequence in fragments shorter than     453 bp
  50% of sequence in fragments shorter than     505 bp
  55% of sequence in fragments shorter than     559 bp
  60% of sequence in fragments shorter than     618 bp
  65% of sequence in fragments shorter than     685 bp
  70% of sequence in fragments shorter than     759 bp
  75% of sequence in fragments shorter than     847 bp
  80% of sequence in fragments shorter than     952 bp
  85% of sequence in fragments shorter than    1080 bp
  90% of sequence in fragments shorter than    1267 bp
  95% of sequence in fragments shorter than    1588 bp

# HhaI.HinfI (GCGC+GANTC) double digest, 10672 thousand fragments (1756502+8916054 sites), 279 bp average fragment length
   5% of sequence in fragments shorter than      91 bp
  10% of sequence in fragments shorter than     146 bp
  15% of sequence in fragments shorter than     188 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     272 bp
  30% of sequence in fragments shorter than     314 bp
  35% of sequence in fragments shorter than     350 bp
  40% of sequence in fragments shorter than     394 bp
  45% of sequence in fragments shorter than     443 bp
  50% of sequence in fragments shorter than     492 bp
  55% of sequence in fragments shorter than     546 bp
  60% of sequence in fragments shorter than     605 bp
  65% of sequence in fragments shorter than     669 bp
  70% of sequence in fragments shorter than     740 bp
  75% of sequence in fragments shorter than     823 bp
  80% of sequence in fragments shorter than     925 bp
  85% of sequence in fragments shorter than    1053 bp
  90% of sequence in fragments shorter than    1230 bp
  95% of sequence in fragments shorter than    1531 bp

# BsmAI.Tsp45I (GTCTC+GTSAC) double digest, 10652 thousand fragments (6631585+4021761 sites), 279 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     184 bp
  20% of sequence in fragments shorter than     226 bp
  25% of sequence in fragments shorter than     272 bp
  30% of sequence in fragments shorter than     317 bp
  35% of sequence in fragments shorter than     361 bp
  40% of sequence in fragments shorter than     409 bp
  45% of sequence in fragments shorter than     460 bp
  50% of sequence in fragments shorter than     514 bp
  55% of sequence in fragments shorter than     571 bp
  60% of sequence in fragments shorter than     633 bp
  65% of sequence in fragments shorter than     704 bp
  70% of sequence in fragments shorter than     788 bp
  75% of sequence in fragments shorter than     886 bp
  80% of sequence in fragments shorter than    1007 bp
  85% of sequence in fragments shorter than    1165 bp
  90% of sequence in fragments shorter than    1391 bp
  95% of sequence in fragments shorter than    1812 bp

# BfiI.Hpy188I (ACTGGG+TCNGA) double digest, 10648 thousand fragments (1706594+8942153 sites), 279 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     144 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     224 bp
  25% of sequence in fragments shorter than     263 bp
  30% of sequence in fragments shorter than     306 bp
  35% of sequence in fragments shorter than     346 bp
  40% of sequence in fragments shorter than     390 bp
  45% of sequence in fragments shorter than     437 bp
  50% of sequence in fragments shorter than     486 bp
  55% of sequence in fragments shorter than     539 bp
  60% of sequence in fragments shorter than     596 bp
  65% of sequence in fragments shorter than     661 bp
  70% of sequence in fragments shorter than     734 bp
  75% of sequence in fragments shorter than     818 bp
  80% of sequence in fragments shorter than     920 bp
  85% of sequence in fragments shorter than    1049 bp
  90% of sequence in fragments shorter than    1226 bp
  95% of sequence in fragments shorter than    1513 bp

# FauI.MboII (CCCGC+GAAGA) double digest, 10638 thousand fragments (1439772+9199496 sites), 280 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     190 bp
  20% of sequence in fragments shorter than     235 bp
  25% of sequence in fragments shorter than     279 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     411 bp
  45% of sequence in fragments shorter than     459 bp
  50% of sequence in fragments shorter than     511 bp
  55% of sequence in fragments shorter than     565 bp
  60% of sequence in fragments shorter than     625 bp
  65% of sequence in fragments shorter than     692 bp
  70% of sequence in fragments shorter than     766 bp
  75% of sequence in fragments shorter than     852 bp
  80% of sequence in fragments shorter than     955 bp
  85% of sequence in fragments shorter than    1078 bp
  90% of sequence in fragments shorter than    1256 bp
  95% of sequence in fragments shorter than    1556 bp

# BspMI.MboII (ACCTGC+GAAGA) double digest, 10637 thousand fragments (1438129+9199496 sites), 280 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     149 bp
  15% of sequence in fragments shorter than     189 bp
  20% of sequence in fragments shorter than     234 bp
  25% of sequence in fragments shorter than     275 bp
  30% of sequence in fragments shorter than     319 bp
  35% of sequence in fragments shorter than     361 bp
  40% of sequence in fragments shorter than     408 bp
  45% of sequence in fragments shorter than     454 bp
  50% of sequence in fragments shorter than     506 bp
  55% of sequence in fragments shorter than     559 bp
  60% of sequence in fragments shorter than     619 bp
  65% of sequence in fragments shorter than     685 bp
  70% of sequence in fragments shorter than     756 bp
  75% of sequence in fragments shorter than     841 bp
  80% of sequence in fragments shorter than     945 bp
  85% of sequence in fragments shorter than    1070 bp
  90% of sequence in fragments shorter than    1251 bp
  95% of sequence in fragments shorter than    1560 bp

# BglII.BstNI (AGATCT+CCWGG) double digest, 10629 thousand fragments (774732+9855646 sites), 280 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     220 bp
  25% of sequence in fragments shorter than     272 bp
  30% of sequence in fragments shorter than     326 bp
  35% of sequence in fragments shorter than     382 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     663 bp
  60% of sequence in fragments shorter than     753 bp
  65% of sequence in fragments shorter than     857 bp
  70% of sequence in fragments shorter than     981 bp
  75% of sequence in fragments shorter than    1130 bp
  80% of sequence in fragments shorter than    1319 bp
  85% of sequence in fragments shorter than    1575 bp
  90% of sequence in fragments shorter than    1957 bp
  95% of sequence in fragments shorter than    2692 bp

# BfiI.HinfI (ACTGGG+GANTC) double digest, 10622 thousand fragments (1706594+8916054 sites), 280 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     189 bp
  20% of sequence in fragments shorter than     231 bp
  25% of sequence in fragments shorter than     271 bp
  30% of sequence in fragments shorter than     311 bp
  35% of sequence in fragments shorter than     345 bp
  40% of sequence in fragments shorter than     381 bp
  45% of sequence in fragments shorter than     426 bp
  50% of sequence in fragments shorter than     472 bp
  55% of sequence in fragments shorter than     523 bp
  60% of sequence in fragments shorter than     577 bp
  65% of sequence in fragments shorter than     637 bp
  70% of sequence in fragments shorter than     700 bp
  75% of sequence in fragments shorter than     778 bp
  80% of sequence in fragments shorter than     869 bp
  85% of sequence in fragments shorter than     986 bp
  90% of sequence in fragments shorter than    1148 bp
  95% of sequence in fragments shorter than    1426 bp

# BstNI.NcoI (CCWGG+CCATGG) double digest, 10614 thousand fragments (9855646+759594 sites), 280 bp average fragment length
   5% of sequence in fragments shorter than      79 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     170 bp
  20% of sequence in fragments shorter than     221 bp
  25% of sequence in fragments shorter than     274 bp
  30% of sequence in fragments shorter than     330 bp
  35% of sequence in fragments shorter than     389 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     523 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     682 bp
  60% of sequence in fragments shorter than     777 bp
  65% of sequence in fragments shorter than     888 bp
  70% of sequence in fragments shorter than    1018 bp
  75% of sequence in fragments shorter than    1174 bp
  80% of sequence in fragments shorter than    1370 bp
  85% of sequence in fragments shorter than    1626 bp
  90% of sequence in fragments shorter than    1975 bp
  95% of sequence in fragments shorter than    2713 bp

# BclI.BstNI (TGATCA+CCWGG) double digest, 10593 thousand fragments (738785+9855646 sites), 281 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     222 bp
  25% of sequence in fragments shorter than     275 bp
  30% of sequence in fragments shorter than     329 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     448 bp
  45% of sequence in fragments shorter than     516 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     668 bp
  60% of sequence in fragments shorter than     760 bp
  65% of sequence in fragments shorter than     864 bp
  70% of sequence in fragments shorter than     988 bp
  75% of sequence in fragments shorter than    1136 bp
  80% of sequence in fragments shorter than    1322 bp
  85% of sequence in fragments shorter than    1572 bp
  90% of sequence in fragments shorter than    1946 bp
  95% of sequence in fragments shorter than    2662 bp

# Hin4II.SfeI (CCTTC+CTRYAG) double digest, 10590 thousand fragments (7059915+3531009 sites), 281 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     279 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     362 bp
  40% of sequence in fragments shorter than     408 bp
  45% of sequence in fragments shorter than     454 bp
  50% of sequence in fragments shorter than     504 bp
  55% of sequence in fragments shorter than     559 bp
  60% of sequence in fragments shorter than     619 bp
  65% of sequence in fragments shorter than     684 bp
  70% of sequence in fragments shorter than     760 bp
  75% of sequence in fragments shorter than     846 bp
  80% of sequence in fragments shorter than     952 bp
  85% of sequence in fragments shorter than    1085 bp
  90% of sequence in fragments shorter than    1270 bp
  95% of sequence in fragments shorter than    1588 bp

# Hpy188I.PsiI (TCNGA+TTATAA) double digest, 10589 thousand fragments (8942153+1647912 sites), 281 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     145 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     224 bp
  25% of sequence in fragments shorter than     265 bp
  30% of sequence in fragments shorter than     308 bp
  35% of sequence in fragments shorter than     347 bp
  40% of sequence in fragments shorter than     391 bp
  45% of sequence in fragments shorter than     437 bp
  50% of sequence in fragments shorter than     486 bp
  55% of sequence in fragments shorter than     539 bp
  60% of sequence in fragments shorter than     595 bp
  65% of sequence in fragments shorter than     659 bp
  70% of sequence in fragments shorter than     732 bp
  75% of sequence in fragments shorter than     816 bp
  80% of sequence in fragments shorter than     917 bp
  85% of sequence in fragments shorter than    1045 bp
  90% of sequence in fragments shorter than    1220 bp
  95% of sequence in fragments shorter than    1511 bp

# BstNI.FnuDII (CCWGG+CGCG) double digest, 10589 thousand fragments (9855646+733940 sites), 281 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     123 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     227 bp
  25% of sequence in fragments shorter than     284 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     410 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     559 bp
  50% of sequence in fragments shorter than     644 bp
  55% of sequence in fragments shorter than     735 bp
  60% of sequence in fragments shorter than     843 bp
  65% of sequence in fragments shorter than     969 bp
  70% of sequence in fragments shorter than    1114 bp
  75% of sequence in fragments shorter than    1292 bp
  80% of sequence in fragments shorter than    1518 bp
  85% of sequence in fragments shorter than    1823 bp
  90% of sequence in fragments shorter than    2254 bp
  95% of sequence in fragments shorter than    3100 bp

# AciI.ApoI (CCGC+RAATTY) double digest, 10588 thousand fragments (4206130+6382433 sites), 281 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     197 bp
  20% of sequence in fragments shorter than     242 bp
  25% of sequence in fragments shorter than     287 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     378 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     475 bp
  50% of sequence in fragments shorter than     528 bp
  55% of sequence in fragments shorter than     584 bp
  60% of sequence in fragments shorter than     646 bp
  65% of sequence in fragments shorter than     713 bp
  70% of sequence in fragments shorter than     789 bp
  75% of sequence in fragments shorter than     879 bp
  80% of sequence in fragments shorter than     987 bp
  85% of sequence in fragments shorter than    1125 bp
  90% of sequence in fragments shorter than    1316 bp
  95% of sequence in fragments shorter than    1643 bp

# BsaAI.BstNI (YACGTR+CCWGG) double digest, 10568 thousand fragments (713801+9855646 sites), 281 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     222 bp
  25% of sequence in fragments shorter than     275 bp
  30% of sequence in fragments shorter than     330 bp
  35% of sequence in fragments shorter than     388 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     521 bp
  50% of sequence in fragments shorter than     597 bp
  55% of sequence in fragments shorter than     678 bp
  60% of sequence in fragments shorter than     770 bp
  65% of sequence in fragments shorter than     880 bp
  70% of sequence in fragments shorter than    1007 bp
  75% of sequence in fragments shorter than    1156 bp
  80% of sequence in fragments shorter than    1345 bp
  85% of sequence in fragments shorter than    1578 bp
  90% of sequence in fragments shorter than    1930 bp
  95% of sequence in fragments shorter than    2576 bp

# HinfI.PsiI (GANTC+TTATAA) double digest, 10563 thousand fragments (8916054+1647912 sites), 282 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     190 bp
  20% of sequence in fragments shorter than     231 bp
  25% of sequence in fragments shorter than     270 bp
  30% of sequence in fragments shorter than     309 bp
  35% of sequence in fragments shorter than     344 bp
  40% of sequence in fragments shorter than     379 bp
  45% of sequence in fragments shorter than     422 bp
  50% of sequence in fragments shorter than     467 bp
  55% of sequence in fragments shorter than     515 bp
  60% of sequence in fragments shorter than     567 bp
  65% of sequence in fragments shorter than     624 bp
  70% of sequence in fragments shorter than     687 bp
  75% of sequence in fragments shorter than     759 bp
  80% of sequence in fragments shorter than     846 bp
  85% of sequence in fragments shorter than     958 bp
  90% of sequence in fragments shorter than    1111 bp
  95% of sequence in fragments shorter than    1363 bp

# BsmAI.MlyI (GTCTC+GAGTC) double digest, 10562 thousand fragments (6631585+3931692 sites), 282 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     193 bp
  20% of sequence in fragments shorter than     238 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     337 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     494 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     616 bp
  60% of sequence in fragments shorter than     687 bp
  65% of sequence in fragments shorter than     768 bp
  70% of sequence in fragments shorter than     861 bp
  75% of sequence in fragments shorter than     971 bp
  80% of sequence in fragments shorter than    1108 bp
  85% of sequence in fragments shorter than    1290 bp
  90% of sequence in fragments shorter than    1555 bp
  95% of sequence in fragments shorter than    2033 bp

# BceAI.BstNI (ACGGC+CCWGG) double digest, 10560 thousand fragments (705178+9855646 sites), 282 bp average fragment length
   5% of sequence in fragments shorter than      79 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     223 bp
  25% of sequence in fragments shorter than     278 bp
  30% of sequence in fragments shorter than     335 bp
  35% of sequence in fragments shorter than     397 bp
  40% of sequence in fragments shorter than     465 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     618 bp
  55% of sequence in fragments shorter than     706 bp
  60% of sequence in fragments shorter than     806 bp
  65% of sequence in fragments shorter than     925 bp
  70% of sequence in fragments shorter than    1063 bp
  75% of sequence in fragments shorter than    1232 bp
  80% of sequence in fragments shorter than    1442 bp
  85% of sequence in fragments shorter than    1728 bp
  90% of sequence in fragments shorter than    2145 bp
  95% of sequence in fragments shorter than    2936 bp

# BciVI.BstNI (GTATCC+CCWGG) double digest, 10551 thousand fragments (696093+9855646 sites), 282 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     126 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     222 bp
  25% of sequence in fragments shorter than     275 bp
  30% of sequence in fragments shorter than     330 bp
  35% of sequence in fragments shorter than     388 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     519 bp
  50% of sequence in fragments shorter than     595 bp
  55% of sequence in fragments shorter than     674 bp
  60% of sequence in fragments shorter than     765 bp
  65% of sequence in fragments shorter than     872 bp
  70% of sequence in fragments shorter than     998 bp
  75% of sequence in fragments shorter than    1146 bp
  80% of sequence in fragments shorter than    1336 bp
  85% of sequence in fragments shorter than    1587 bp
  90% of sequence in fragments shorter than    1949 bp
  95% of sequence in fragments shorter than    2639 bp

# EsaBC3I.Hpy188I (TCGA+TCNGA) double digest, 10544 thousand fragments (1603346+8942153 sites), 282 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     146 bp
  15% of sequence in fragments shorter than     185 bp
  20% of sequence in fragments shorter than     227 bp
  25% of sequence in fragments shorter than     268 bp
  30% of sequence in fragments shorter than     309 bp
  35% of sequence in fragments shorter than     347 bp
  40% of sequence in fragments shorter than     390 bp
  45% of sequence in fragments shorter than     435 bp
  50% of sequence in fragments shorter than     483 bp
  55% of sequence in fragments shorter than     535 bp
  60% of sequence in fragments shorter than     590 bp
  65% of sequence in fragments shorter than     653 bp
  70% of sequence in fragments shorter than     724 bp
  75% of sequence in fragments shorter than     805 bp
  80% of sequence in fragments shorter than     902 bp
  85% of sequence in fragments shorter than    1023 bp
  90% of sequence in fragments shorter than    1186 bp
  95% of sequence in fragments shorter than    1459 bp

# HhaI.TspRI (GCGC+CASTG) double digest, 10521 thousand fragments (1756502+8765240 sites), 283 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     152 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     286 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     426 bp
  45% of sequence in fragments shorter than     479 bp
  50% of sequence in fragments shorter than     534 bp
  55% of sequence in fragments shorter than     595 bp
  60% of sequence in fragments shorter than     660 bp
  65% of sequence in fragments shorter than     731 bp
  70% of sequence in fragments shorter than     814 bp
  75% of sequence in fragments shorter than     912 bp
  80% of sequence in fragments shorter than    1024 bp
  85% of sequence in fragments shorter than    1169 bp
  90% of sequence in fragments shorter than    1380 bp
  95% of sequence in fragments shorter than    1727 bp

# MboII.PstI (GAAGA+CTGCAG) double digest, 10520 thousand fragments (9199496+1321469 sites), 283 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     191 bp
  20% of sequence in fragments shorter than     236 bp
  25% of sequence in fragments shorter than     280 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     366 bp
  40% of sequence in fragments shorter than     414 bp
  45% of sequence in fragments shorter than     462 bp
  50% of sequence in fragments shorter than     515 bp
  55% of sequence in fragments shorter than     570 bp
  60% of sequence in fragments shorter than     631 bp
  65% of sequence in fragments shorter than     698 bp
  70% of sequence in fragments shorter than     774 bp
  75% of sequence in fragments shorter than     861 bp
  80% of sequence in fragments shorter than     966 bp
  85% of sequence in fragments shorter than    1092 bp
  90% of sequence in fragments shorter than    1276 bp
  95% of sequence in fragments shorter than    1588 bp

# EsaBC3I.HinfI (TCGA+GANTC) double digest, 10518 thousand fragments (1603346+8916054 sites), 283 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     148 bp
  15% of sequence in fragments shorter than     192 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     273 bp
  30% of sequence in fragments shorter than     314 bp
  35% of sequence in fragments shorter than     348 bp
  40% of sequence in fragments shorter than     390 bp
  45% of sequence in fragments shorter than     436 bp
  50% of sequence in fragments shorter than     484 bp
  55% of sequence in fragments shorter than     536 bp
  60% of sequence in fragments shorter than     593 bp
  65% of sequence in fragments shorter than     656 bp
  70% of sequence in fragments shorter than     721 bp
  75% of sequence in fragments shorter than     801 bp
  80% of sequence in fragments shorter than     898 bp
  85% of sequence in fragments shorter than    1020 bp
  90% of sequence in fragments shorter than    1188 bp
  95% of sequence in fragments shorter than    1472 bp

# AflII.BstNI (CTTAAG+CCWGG) double digest, 10498 thousand fragments (643284+9855646 sites), 283 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     126 bp
  15% of sequence in fragments shorter than     171 bp
  20% of sequence in fragments shorter than     223 bp
  25% of sequence in fragments shorter than     276 bp
  30% of sequence in fragments shorter than     330 bp
  35% of sequence in fragments shorter than     389 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     520 bp
  50% of sequence in fragments shorter than     596 bp
  55% of sequence in fragments shorter than     675 bp
  60% of sequence in fragments shorter than     766 bp
  65% of sequence in fragments shorter than     873 bp
  70% of sequence in fragments shorter than    1000 bp
  75% of sequence in fragments shorter than    1151 bp
  80% of sequence in fragments shorter than    1341 bp
  85% of sequence in fragments shorter than    1600 bp
  90% of sequence in fragments shorter than    1984 bp
  95% of sequence in fragments shorter than    2722 bp

# HgiCI.Hpy188I (GGYRCC+TCNGA) double digest, 10492 thousand fragments (1550877+8942153 sites), 284 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     148 bp
  15% of sequence in fragments shorter than     185 bp
  20% of sequence in fragments shorter than     227 bp
  25% of sequence in fragments shorter than     267 bp
  30% of sequence in fragments shorter than     310 bp
  35% of sequence in fragments shorter than     347 bp
  40% of sequence in fragments shorter than     391 bp
  45% of sequence in fragments shorter than     437 bp
  50% of sequence in fragments shorter than     486 bp
  55% of sequence in fragments shorter than     539 bp
  60% of sequence in fragments shorter than     596 bp
  65% of sequence in fragments shorter than     661 bp
  70% of sequence in fragments shorter than     734 bp
  75% of sequence in fragments shorter than     817 bp
  80% of sequence in fragments shorter than     918 bp
  85% of sequence in fragments shorter than    1044 bp
  90% of sequence in fragments shorter than    1210 bp
  95% of sequence in fragments shorter than    1494 bp

# BsmI.Hpy188I (GAATGC+TCNGA) double digest, 10490 thousand fragments (1549350+8942153 sites), 284 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     188 bp
  20% of sequence in fragments shorter than     233 bp
  25% of sequence in fragments shorter than     277 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     365 bp
  40% of sequence in fragments shorter than     413 bp
  45% of sequence in fragments shorter than     461 bp
  50% of sequence in fragments shorter than     511 bp
  55% of sequence in fragments shorter than     568 bp
  60% of sequence in fragments shorter than     628 bp
  65% of sequence in fragments shorter than     696 bp
  70% of sequence in fragments shorter than     773 bp
  75% of sequence in fragments shorter than     860 bp
  80% of sequence in fragments shorter than     966 bp
  85% of sequence in fragments shorter than    1099 bp
  90% of sequence in fragments shorter than    1284 bp
  95% of sequence in fragments shorter than    1598 bp

# BbvI.FokI (GCAGC+GGATG) double digest, 10490 thousand fragments (5290051+5201116 sites), 284 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     196 bp
  20% of sequence in fragments shorter than     244 bp
  25% of sequence in fragments shorter than     292 bp
  30% of sequence in fragments shorter than     342 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     447 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     565 bp
  55% of sequence in fragments shorter than     631 bp
  60% of sequence in fragments shorter than     705 bp
  65% of sequence in fragments shorter than     787 bp
  70% of sequence in fragments shorter than     874 bp
  75% of sequence in fragments shorter than     983 bp
  80% of sequence in fragments shorter than    1115 bp
  85% of sequence in fragments shorter than    1283 bp
  90% of sequence in fragments shorter than    1508 bp
  95% of sequence in fragments shorter than    1916 bp

# BstNI.HaeII (CCWGG+RGCGCY) double digest, 10485 thousand fragments (9855646+630101 sites), 284 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     229 bp
  25% of sequence in fragments shorter than     286 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     405 bp
  40% of sequence in fragments shorter than     475 bp
  45% of sequence in fragments shorter than     550 bp
  50% of sequence in fragments shorter than     631 bp
  55% of sequence in fragments shorter than     714 bp
  60% of sequence in fragments shorter than     817 bp
  65% of sequence in fragments shorter than     936 bp
  70% of sequence in fragments shorter than    1070 bp
  75% of sequence in fragments shorter than    1238 bp
  80% of sequence in fragments shorter than    1441 bp
  85% of sequence in fragments shorter than    1715 bp
  90% of sequence in fragments shorter than    2088 bp
  95% of sequence in fragments shorter than    2731 bp

# BfiI.TspRI (ACTGGG+CASTG) double digest, 10471 thousand fragments (1706594+8765240 sites), 284 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     291 bp
  30% of sequence in fragments shorter than     335 bp
  35% of sequence in fragments shorter than     379 bp
  40% of sequence in fragments shorter than     427 bp
  45% of sequence in fragments shorter than     477 bp
  50% of sequence in fragments shorter than     531 bp
  55% of sequence in fragments shorter than     589 bp
  60% of sequence in fragments shorter than     653 bp
  65% of sequence in fragments shorter than     722 bp
  70% of sequence in fragments shorter than     803 bp
  75% of sequence in fragments shorter than     898 bp
  80% of sequence in fragments shorter than    1009 bp
  85% of sequence in fragments shorter than    1154 bp
  90% of sequence in fragments shorter than    1363 bp
  95% of sequence in fragments shorter than    1724 bp

# HgiCI.HinfI (GGYRCC+GANTC) double digest, 10466 thousand fragments (1550877+8916054 sites), 284 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     145 bp
  15% of sequence in fragments shorter than     189 bp
  20% of sequence in fragments shorter than     231 bp
  25% of sequence in fragments shorter than     273 bp
  30% of sequence in fragments shorter than     314 bp
  35% of sequence in fragments shorter than     347 bp
  40% of sequence in fragments shorter than     389 bp
  45% of sequence in fragments shorter than     435 bp
  50% of sequence in fragments shorter than     483 bp
  55% of sequence in fragments shorter than     535 bp
  60% of sequence in fragments shorter than     593 bp
  65% of sequence in fragments shorter than     656 bp
  70% of sequence in fragments shorter than     722 bp
  75% of sequence in fragments shorter than     802 bp
  80% of sequence in fragments shorter than     900 bp
  85% of sequence in fragments shorter than    1023 bp
  90% of sequence in fragments shorter than    1193 bp
  95% of sequence in fragments shorter than    1483 bp

# BsmI.HinfI (GAATGC+GANTC) double digest, 10464 thousand fragments (1549350+8916054 sites), 284 bp average fragment length
   5% of sequence in fragments shorter than      92 bp
  10% of sequence in fragments shorter than     144 bp
  15% of sequence in fragments shorter than     191 bp
  20% of sequence in fragments shorter than     235 bp
  25% of sequence in fragments shorter than     276 bp
  30% of sequence in fragments shorter than     316 bp
  35% of sequence in fragments shorter than     353 bp
  40% of sequence in fragments shorter than     395 bp
  45% of sequence in fragments shorter than     441 bp
  50% of sequence in fragments shorter than     488 bp
  55% of sequence in fragments shorter than     539 bp
  60% of sequence in fragments shorter than     595 bp
  65% of sequence in fragments shorter than     657 bp
  70% of sequence in fragments shorter than     725 bp
  75% of sequence in fragments shorter than     804 bp
  80% of sequence in fragments shorter than     899 bp
  85% of sequence in fragments shorter than    1020 bp
  90% of sequence in fragments shorter than    1187 bp
  95% of sequence in fragments shorter than    1468 bp

# BstNI.SacI (CCWGG+GAGCTC) double digest, 10458 thousand fragments (9855646+603323 sites), 284 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     172 bp
  20% of sequence in fragments shorter than     225 bp
  25% of sequence in fragments shorter than     281 bp
  30% of sequence in fragments shorter than     338 bp
  35% of sequence in fragments shorter than     400 bp
  40% of sequence in fragments shorter than     467 bp
  45% of sequence in fragments shorter than     541 bp
  50% of sequence in fragments shorter than     620 bp
  55% of sequence in fragments shorter than     707 bp
  60% of sequence in fragments shorter than     807 bp
  65% of sequence in fragments shorter than     925 bp
  70% of sequence in fragments shorter than    1063 bp
  75% of sequence in fragments shorter than    1230 bp
  80% of sequence in fragments shorter than    1440 bp
  85% of sequence in fragments shorter than    1728 bp
  90% of sequence in fragments shorter than    2139 bp
  95% of sequence in fragments shorter than    2928 bp

# AvrII.BstNI (CCTAGG+CCWGG) double digest, 10450 thousand fragments (594956+9855646 sites), 285 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     172 bp
  20% of sequence in fragments shorter than     225 bp
  25% of sequence in fragments shorter than     280 bp
  30% of sequence in fragments shorter than     336 bp
  35% of sequence in fragments shorter than     397 bp
  40% of sequence in fragments shorter than     464 bp
  45% of sequence in fragments shorter than     537 bp
  50% of sequence in fragments shorter than     614 bp
  55% of sequence in fragments shorter than     694 bp
  60% of sequence in fragments shorter than     792 bp
  65% of sequence in fragments shorter than     907 bp
  70% of sequence in fragments shorter than    1035 bp
  75% of sequence in fragments shorter than    1195 bp
  80% of sequence in fragments shorter than    1390 bp
  85% of sequence in fragments shorter than    1651 bp
  90% of sequence in fragments shorter than    2044 bp
  95% of sequence in fragments shorter than    2830 bp

# BscGI.MboII (CCCGT+GAAGA) double digest, 10430 thousand fragments (1231070+9199496 sites), 285 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     154 bp
  15% of sequence in fragments shorter than     194 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     284 bp
  30% of sequence in fragments shorter than     327 bp
  35% of sequence in fragments shorter than     370 bp
  40% of sequence in fragments shorter than     418 bp
  45% of sequence in fragments shorter than     466 bp
  50% of sequence in fragments shorter than     518 bp
  55% of sequence in fragments shorter than     574 bp
  60% of sequence in fragments shorter than     635 bp
  65% of sequence in fragments shorter than     703 bp
  70% of sequence in fragments shorter than     778 bp
  75% of sequence in fragments shorter than     864 bp
  80% of sequence in fragments shorter than     969 bp
  85% of sequence in fragments shorter than    1094 bp
  90% of sequence in fragments shorter than    1274 bp
  95% of sequence in fragments shorter than    1578 bp

# BstNI.HgaI (CCWGG+GACGC) double digest, 10427 thousand fragments (9855646+571889 sites), 285 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     125 bp
  15% of sequence in fragments shorter than     172 bp
  20% of sequence in fragments shorter than     226 bp
  25% of sequence in fragments shorter than     283 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     473 bp
  45% of sequence in fragments shorter than     548 bp
  50% of sequence in fragments shorter than     630 bp
  55% of sequence in fragments shorter than     719 bp
  60% of sequence in fragments shorter than     823 bp
  65% of sequence in fragments shorter than     945 bp
  70% of sequence in fragments shorter than    1086 bp
  75% of sequence in fragments shorter than    1258 bp
  80% of sequence in fragments shorter than    1473 bp
  85% of sequence in fragments shorter than    1767 bp
  90% of sequence in fragments shorter than    2182 bp
  95% of sequence in fragments shorter than    2953 bp

# AflIII.Hpy188I (ACRYGT+TCNGA) double digest, 10427 thousand fragments (1485394+8942153 sites), 285 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     149 bp
  15% of sequence in fragments shorter than     186 bp
  20% of sequence in fragments shorter than     229 bp
  25% of sequence in fragments shorter than     272 bp
  30% of sequence in fragments shorter than     315 bp
  35% of sequence in fragments shorter than     354 bp
  40% of sequence in fragments shorter than     399 bp
  45% of sequence in fragments shorter than     446 bp
  50% of sequence in fragments shorter than     496 bp
  55% of sequence in fragments shorter than     550 bp
  60% of sequence in fragments shorter than     607 bp
  65% of sequence in fragments shorter than     672 bp
  70% of sequence in fragments shorter than     745 bp
  75% of sequence in fragments shorter than     830 bp
  80% of sequence in fragments shorter than     932 bp
  85% of sequence in fragments shorter than    1062 bp
  90% of sequence in fragments shorter than    1240 bp
  95% of sequence in fragments shorter than    1532 bp

# BstNI.EciI (CCWGG+GGCGGA) double digest, 10426 thousand fragments (9855646+571273 sites), 285 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     126 bp
  15% of sequence in fragments shorter than     174 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     289 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     414 bp
  40% of sequence in fragments shorter than     486 bp
  45% of sequence in fragments shorter than     564 bp
  50% of sequence in fragments shorter than     648 bp
  55% of sequence in fragments shorter than     740 bp
  60% of sequence in fragments shorter than     848 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1120 bp
  75% of sequence in fragments shorter than    1296 bp
  80% of sequence in fragments shorter than    1522 bp
  85% of sequence in fragments shorter than    1826 bp
  90% of sequence in fragments shorter than    2256 bp
  95% of sequence in fragments shorter than    3113 bp

# BstNI.MfeI (CCWGG+CAATTG) double digest, 10419 thousand fragments (9855646+564303 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     127 bp
  15% of sequence in fragments shorter than     174 bp
  20% of sequence in fragments shorter than     226 bp
  25% of sequence in fragments shorter than     279 bp
  30% of sequence in fragments shorter than     334 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     524 bp
  50% of sequence in fragments shorter than     598 bp
  55% of sequence in fragments shorter than     678 bp
  60% of sequence in fragments shorter than     768 bp
  65% of sequence in fragments shorter than     874 bp
  70% of sequence in fragments shorter than     997 bp
  75% of sequence in fragments shorter than    1141 bp
  80% of sequence in fragments shorter than    1326 bp
  85% of sequence in fragments shorter than    1575 bp
  90% of sequence in fragments shorter than    1927 bp
  95% of sequence in fragments shorter than    2590 bp

# AciI.BthCI (CCGC+GCNGC) double digest, 10415 thousand fragments (4206130+6209929 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     240 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     377 bp
  30% of sequence in fragments shorter than     450 bp
  35% of sequence in fragments shorter than     527 bp
  40% of sequence in fragments shorter than     606 bp
  45% of sequence in fragments shorter than     690 bp
  50% of sequence in fragments shorter than     783 bp
  55% of sequence in fragments shorter than     877 bp
  60% of sequence in fragments shorter than     986 bp
  65% of sequence in fragments shorter than    1106 bp
  70% of sequence in fragments shorter than    1250 bp
  75% of sequence in fragments shorter than    1417 bp
  80% of sequence in fragments shorter than    1622 bp
  85% of sequence in fragments shorter than    1886 bp
  90% of sequence in fragments shorter than    2255 bp
  95% of sequence in fragments shorter than    2905 bp

# PsiI.TspRI (TTATAA+CASTG) double digest, 10412 thousand fragments (1647912+8765240 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     235 bp
  25% of sequence in fragments shorter than     274 bp
  30% of sequence in fragments shorter than     314 bp
  35% of sequence in fragments shorter than     354 bp
  40% of sequence in fragments shorter than     397 bp
  45% of sequence in fragments shorter than     442 bp
  50% of sequence in fragments shorter than     490 bp
  55% of sequence in fragments shorter than     540 bp
  60% of sequence in fragments shorter than     596 bp
  65% of sequence in fragments shorter than     658 bp
  70% of sequence in fragments shorter than     727 bp
  75% of sequence in fragments shorter than     809 bp
  80% of sequence in fragments shorter than     910 bp
  85% of sequence in fragments shorter than    1033 bp
  90% of sequence in fragments shorter than    1210 bp
  95% of sequence in fragments shorter than    1519 bp

# BstNI.SphI (CCWGG+GCATGC) double digest, 10406 thousand fragments (9855646+550952 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than      80 bp
  10% of sequence in fragments shorter than     126 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     227 bp
  25% of sequence in fragments shorter than     283 bp
  30% of sequence in fragments shorter than     340 bp
  35% of sequence in fragments shorter than     401 bp
  40% of sequence in fragments shorter than     469 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     621 bp
  55% of sequence in fragments shorter than     708 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     926 bp
  70% of sequence in fragments shorter than    1062 bp
  75% of sequence in fragments shorter than    1229 bp
  80% of sequence in fragments shorter than    1437 bp
  85% of sequence in fragments shorter than    1723 bp
  90% of sequence in fragments shorter than    2133 bp
  95% of sequence in fragments shorter than    2921 bp

# BbvI.CviQI (GCAGC+GTAC) double digest, 10404 thousand fragments (5290051+5115078 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     199 bp
  20% of sequence in fragments shorter than     244 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     331 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     474 bp
  50% of sequence in fragments shorter than     528 bp
  55% of sequence in fragments shorter than     583 bp
  60% of sequence in fragments shorter than     643 bp
  65% of sequence in fragments shorter than     710 bp
  70% of sequence in fragments shorter than     789 bp
  75% of sequence in fragments shorter than     877 bp
  80% of sequence in fragments shorter than     982 bp
  85% of sequence in fragments shorter than    1115 bp
  90% of sequence in fragments shorter than    1304 bp
  95% of sequence in fragments shorter than    1627 bp

# ApoI.Tsp45I (RAATTY+GTSAC) double digest, 10403 thousand fragments (6382433+4021761 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     148 bp
  15% of sequence in fragments shorter than     192 bp
  20% of sequence in fragments shorter than     236 bp
  25% of sequence in fragments shorter than     275 bp
  30% of sequence in fragments shorter than     315 bp
  35% of sequence in fragments shorter than     354 bp
  40% of sequence in fragments shorter than     397 bp
  45% of sequence in fragments shorter than     443 bp
  50% of sequence in fragments shorter than     492 bp
  55% of sequence in fragments shorter than     544 bp
  60% of sequence in fragments shorter than     601 bp
  65% of sequence in fragments shorter than     665 bp
  70% of sequence in fragments shorter than     734 bp
  75% of sequence in fragments shorter than     818 bp
  80% of sequence in fragments shorter than     915 bp
  85% of sequence in fragments shorter than    1041 bp
  90% of sequence in fragments shorter than    1220 bp
  95% of sequence in fragments shorter than    1500 bp

# AflIII.HinfI (ACRYGT+GANTC) double digest, 10400 thousand fragments (1485394+8916054 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     194 bp
  20% of sequence in fragments shorter than     236 bp
  25% of sequence in fragments shorter than     276 bp
  30% of sequence in fragments shorter than     316 bp
  35% of sequence in fragments shorter than     348 bp
  40% of sequence in fragments shorter than     388 bp
  45% of sequence in fragments shorter than     430 bp
  50% of sequence in fragments shorter than     476 bp
  55% of sequence in fragments shorter than     524 bp
  60% of sequence in fragments shorter than     578 bp
  65% of sequence in fragments shorter than     638 bp
  70% of sequence in fragments shorter than     700 bp
  75% of sequence in fragments shorter than     777 bp
  80% of sequence in fragments shorter than     867 bp
  85% of sequence in fragments shorter than     983 bp
  90% of sequence in fragments shorter than    1139 bp
  95% of sequence in fragments shorter than    1402 bp

# BstNI.ScaI (CCWGG+AGTACT) double digest, 10398 thousand fragments (9855646+543793 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     127 bp
  15% of sequence in fragments shorter than     173 bp
  20% of sequence in fragments shorter than     226 bp
  25% of sequence in fragments shorter than     280 bp
  30% of sequence in fragments shorter than     335 bp
  35% of sequence in fragments shorter than     394 bp
  40% of sequence in fragments shorter than     458 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     602 bp
  55% of sequence in fragments shorter than     684 bp
  60% of sequence in fragments shorter than     776 bp
  65% of sequence in fragments shorter than     886 bp
  70% of sequence in fragments shorter than    1012 bp
  75% of sequence in fragments shorter than    1166 bp
  80% of sequence in fragments shorter than    1360 bp
  85% of sequence in fragments shorter than    1623 bp
  90% of sequence in fragments shorter than    2011 bp
  95% of sequence in fragments shorter than    2744 bp

# BspNCI.StyI (CCAGA+CCWWGG) double digest, 10396 thousand fragments (7282436+3114107 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     192 bp
  20% of sequence in fragments shorter than     236 bp
  25% of sequence in fragments shorter than     280 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     367 bp
  40% of sequence in fragments shorter than     415 bp
  45% of sequence in fragments shorter than     464 bp
  50% of sequence in fragments shorter than     516 bp
  55% of sequence in fragments shorter than     569 bp
  60% of sequence in fragments shorter than     629 bp
  65% of sequence in fragments shorter than     697 bp
  70% of sequence in fragments shorter than     776 bp
  75% of sequence in fragments shorter than     867 bp
  80% of sequence in fragments shorter than     981 bp
  85% of sequence in fragments shorter than    1126 bp
  90% of sequence in fragments shorter than    1332 bp
  95% of sequence in fragments shorter than    1696 bp

# BspNCI.NspI (CCAGA+RCATGY) double digest, 10386 thousand fragments (7282436+3104359 sites), 286 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     194 bp
  20% of sequence in fragments shorter than     236 bp
  25% of sequence in fragments shorter than     278 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     361 bp
  40% of sequence in fragments shorter than     406 bp
  45% of sequence in fragments shorter than     450 bp
  50% of sequence in fragments shorter than     500 bp
  55% of sequence in fragments shorter than     554 bp
  60% of sequence in fragments shorter than     608 bp
  65% of sequence in fragments shorter than     672 bp
  70% of sequence in fragments shorter than     745 bp
  75% of sequence in fragments shorter than     831 bp
  80% of sequence in fragments shorter than     931 bp
  85% of sequence in fragments shorter than    1059 bp
  90% of sequence in fragments shorter than    1230 bp
  95% of sequence in fragments shorter than    1547 bp

# FauI.Hpy188I (CCCGC+TCNGA) double digest, 10381 thousand fragments (1439772+8942153 sites), 287 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     148 bp
  15% of sequence in fragments shorter than     187 bp
  20% of sequence in fragments shorter than     232 bp
  25% of sequence in fragments shorter than     276 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     359 bp
  40% of sequence in fragments shorter than     406 bp
  45% of sequence in fragments shorter than     454 bp
  50% of sequence in fragments shorter than     506 bp
  55% of sequence in fragments shorter than     561 bp
  60% of sequence in fragments shorter than     619 bp
  65% of sequence in fragments shorter than     687 bp
  70% of sequence in fragments shorter than     763 bp
  75% of sequence in fragments shorter than     850 bp
  80% of sequence in fragments shorter than     955 bp
  85% of sequence in fragments shorter than    1085 bp
  90% of sequence in fragments shorter than    1256 bp
  95% of sequence in fragments shorter than    1557 bp

# BspMI.Hpy188I (ACCTGC+TCNGA) double digest, 10379 thousand fragments (1438129+8942153 sites), 287 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     187 bp
  20% of sequence in fragments shorter than     231 bp
  25% of sequence in fragments shorter than     273 bp
  30% of sequence in fragments shorter than     317 bp
  35% of sequence in fragments shorter than     357 bp
  40% of sequence in fragments shorter than     404 bp
  45% of sequence in fragments shorter than     452 bp
  50% of sequence in fragments shorter than     503 bp
  55% of sequence in fragments shorter than     557 bp
  60% of sequence in fragments shorter than     616 bp
  65% of sequence in fragments shorter than     682 bp
  70% of sequence in fragments shorter than     753 bp
  75% of sequence in fragments shorter than     839 bp
  80% of sequence in fragments shorter than     941 bp
  85% of sequence in fragments shorter than    1073 bp
  90% of sequence in fragments shorter than    1250 bp
  95% of sequence in fragments shorter than    1546 bp

# AciI.BccI (CCGC+CCATC) double digest, 10376 thousand fragments (4206130+6170927 sites), 287 bp average fragment length
   5% of sequence in fragments shorter than      91 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     362 bp
  35% of sequence in fragments shorter than     420 bp
  40% of sequence in fragments shorter than     478 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     611 bp
  55% of sequence in fragments shorter than     684 bp
  60% of sequence in fragments shorter than     765 bp
  65% of sequence in fragments shorter than     857 bp
  70% of sequence in fragments shorter than     964 bp
  75% of sequence in fragments shorter than    1083 bp
  80% of sequence in fragments shorter than    1235 bp
  85% of sequence in fragments shorter than    1425 bp
  90% of sequence in fragments shorter than    1702 bp
  95% of sequence in fragments shorter than    2181 bp

# EsaBC3I.TspRI (TCGA+CASTG) double digest, 10368 thousand fragments (1603346+8765240 sites), 287 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     149 bp
  15% of sequence in fragments shorter than     194 bp
  20% of sequence in fragments shorter than     234 bp
  25% of sequence in fragments shorter than     277 bp
  30% of sequence in fragments shorter than     321 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     410 bp
  45% of sequence in fragments shorter than     458 bp
  50% of sequence in fragments shorter than     510 bp
  55% of sequence in fragments shorter than     565 bp
  60% of sequence in fragments shorter than     627 bp
  65% of sequence in fragments shorter than     694 bp
  70% of sequence in fragments shorter than     771 bp
  75% of sequence in fragments shorter than     861 bp
  80% of sequence in fragments shorter than     964 bp
  85% of sequence in fragments shorter than    1098 bp
  90% of sequence in fragments shorter than    1294 bp
  95% of sequence in fragments shorter than    1631 bp

# FauI.HinfI (CCCGC+GANTC) double digest, 10355 thousand fragments (1439772+8916054 sites), 287 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     145 bp
  15% of sequence in fragments shorter than     191 bp
  20% of sequence in fragments shorter than     234 bp
  25% of sequence in fragments shorter than     277 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     356 bp
  40% of sequence in fragments shorter than     401 bp
  45% of sequence in fragments shorter than     449 bp
  50% of sequence in fragments shorter than     500 bp
  55% of sequence in fragments shorter than     554 bp
  60% of sequence in fragments shorter than     615 bp
  65% of sequence in fragments shorter than     679 bp
  70% of sequence in fragments shorter than     750 bp
  75% of sequence in fragments shorter than     834 bp
  80% of sequence in fragments shorter than     938 bp
  85% of sequence in fragments shorter than    1066 bp
  90% of sequence in fragments shorter than    1244 bp
  95% of sequence in fragments shorter than    1547 bp

# BspMI.HinfI (ACCTGC+GANTC) double digest, 10353 thousand fragments (1438129+8916054 sites), 287 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     148 bp
  15% of sequence in fragments shorter than     192 bp
  20% of sequence in fragments shorter than     234 bp
  25% of sequence in fragments shorter than     275 bp
  30% of sequence in fragments shorter than     316 bp
  35% of sequence in fragments shorter than     350 bp
  40% of sequence in fragments shorter than     391 bp
  45% of sequence in fragments shorter than     437 bp
  50% of sequence in fragments shorter than     485 bp
  55% of sequence in fragments shorter than     532 bp
  60% of sequence in fragments shorter than     589 bp
  65% of sequence in fragments shorter than     651 bp
  70% of sequence in fragments shorter than     716 bp
  75% of sequence in fragments shorter than     795 bp
  80% of sequence in fragments shorter than     889 bp
  85% of sequence in fragments shorter than    1010 bp
  90% of sequence in fragments shorter than    1179 bp
  95% of sequence in fragments shorter than    1462 bp

# ApaLI.BstNI (GTGCAC+CCWGG) double digest, 10351 thousand fragments (496255+9855646 sites), 287 bp average fragment length
   5% of sequence in fragments shorter than      81 bp
  10% of sequence in fragments shorter than     127 bp
  15% of sequence in fragments shorter than     174 bp
  20% of sequence in fragments shorter than     228 bp
  25% of sequence in fragments shorter than     285 bp
  30% of sequence in fragments shorter than     342 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     472 bp
  45% of sequence in fragments shorter than     546 bp
  50% of sequence in fragments shorter than     625 bp
  55% of sequence in fragments shorter than     712 bp
  60% of sequence in fragments shorter than     812 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1066 bp
  75% of sequence in fragments shorter than    1231 bp
  80% of sequence in fragments shorter than    1437 bp
  85% of sequence in fragments shorter than    1717 bp
  90% of sequence in fragments shorter than    2078 bp
  95% of sequence in fragments shorter than    2852 bp

# HaeIII.HhaI (GGCC+GCGC) double digest, 10318 thousand fragments (8562230+1756502 sites), 288 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     146 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     262 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     376 bp
  35% of sequence in fragments shorter than     440 bp
  40% of sequence in fragments shorter than     511 bp
  45% of sequence in fragments shorter than     584 bp
  50% of sequence in fragments shorter than     665 bp
  55% of sequence in fragments shorter than     751 bp
  60% of sequence in fragments shorter than     852 bp
  65% of sequence in fragments shorter than     959 bp
  70% of sequence in fragments shorter than    1090 bp
  75% of sequence in fragments shorter than    1244 bp
  80% of sequence in fragments shorter than    1425 bp
  85% of sequence in fragments shorter than    1668 bp
  90% of sequence in fragments shorter than    2007 bp
  95% of sequence in fragments shorter than    2611 bp

# CviQI.FokI (GTAC+GGATG) double digest, 10315 thousand fragments (5115078+5201116 sites), 288 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     280 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     365 bp
  40% of sequence in fragments shorter than     411 bp
  45% of sequence in fragments shorter than     458 bp
  50% of sequence in fragments shorter than     506 bp
  55% of sequence in fragments shorter than     559 bp
  60% of sequence in fragments shorter than     616 bp
  65% of sequence in fragments shorter than     678 bp
  70% of sequence in fragments shorter than     744 bp
  75% of sequence in fragments shorter than     826 bp
  80% of sequence in fragments shorter than     922 bp
  85% of sequence in fragments shorter than    1045 bp
  90% of sequence in fragments shorter than    1218 bp
  95% of sequence in fragments shorter than    1520 bp

# HgiCI.TspRI (GGYRCC+CASTG) double digest, 10315 thousand fragments (1550877+8765240 sites), 288 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     152 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     284 bp
  30% of sequence in fragments shorter than     328 bp
  35% of sequence in fragments shorter than     372 bp
  40% of sequence in fragments shorter than     420 bp
  45% of sequence in fragments shorter than     471 bp
  50% of sequence in fragments shorter than     525 bp
  55% of sequence in fragments shorter than     584 bp
  60% of sequence in fragments shorter than     643 bp
  65% of sequence in fragments shorter than     712 bp
  70% of sequence in fragments shorter than     793 bp
  75% of sequence in fragments shorter than     890 bp
  80% of sequence in fragments shorter than    1002 bp
  85% of sequence in fragments shorter than    1150 bp
  90% of sequence in fragments shorter than    1362 bp
  95% of sequence in fragments shorter than    1750 bp

# ApaI.BstNI (GGGCCC+CCWGG) double digest, 10315 thousand fragments (460339+9855646 sites), 288 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     127 bp
  15% of sequence in fragments shorter than     175 bp
  20% of sequence in fragments shorter than     231 bp
  25% of sequence in fragments shorter than     289 bp
  30% of sequence in fragments shorter than     348 bp
  35% of sequence in fragments shorter than     413 bp
  40% of sequence in fragments shorter than     483 bp
  45% of sequence in fragments shorter than     560 bp
  50% of sequence in fragments shorter than     644 bp
  55% of sequence in fragments shorter than     734 bp
  60% of sequence in fragments shorter than     841 bp
  65% of sequence in fragments shorter than     966 bp
  70% of sequence in fragments shorter than    1111 bp
  75% of sequence in fragments shorter than    1286 bp
  80% of sequence in fragments shorter than    1511 bp
  85% of sequence in fragments shorter than    1816 bp
  90% of sequence in fragments shorter than    2248 bp
  95% of sequence in fragments shorter than    3095 bp

# BsmI.TspRI (GAATGC+CASTG) double digest, 10314 thousand fragments (1549350+8765240 sites), 288 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     237 bp
  25% of sequence in fragments shorter than     280 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     409 bp
  45% of sequence in fragments shorter than     457 bp
  50% of sequence in fragments shorter than     508 bp
  55% of sequence in fragments shorter than     563 bp
  60% of sequence in fragments shorter than     624 bp
  65% of sequence in fragments shorter than     690 bp
  70% of sequence in fragments shorter than     765 bp
  75% of sequence in fragments shorter than     852 bp
  80% of sequence in fragments shorter than     951 bp
  85% of sequence in fragments shorter than    1081 bp
  90% of sequence in fragments shorter than    1262 bp
  95% of sequence in fragments shorter than    1575 bp

# ApoI.MlyI (RAATTY+GAGTC) double digest, 10313 thousand fragments (6382433+3931692 sites), 288 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     195 bp
  20% of sequence in fragments shorter than     238 bp
  25% of sequence in fragments shorter than     278 bp
  30% of sequence in fragments shorter than     316 bp
  35% of sequence in fragments shorter than     355 bp
  40% of sequence in fragments shorter than     397 bp
  45% of sequence in fragments shorter than     442 bp
  50% of sequence in fragments shorter than     490 bp
  55% of sequence in fragments shorter than     540 bp
  60% of sequence in fragments shorter than     596 bp
  65% of sequence in fragments shorter than     658 bp
  70% of sequence in fragments shorter than     727 bp
  75% of sequence in fragments shorter than     811 bp
  80% of sequence in fragments shorter than     907 bp
  85% of sequence in fragments shorter than    1033 bp
  90% of sequence in fragments shorter than    1213 bp
  95% of sequence in fragments shorter than    1502 bp

# BspKT6I.StyI (GATC+CCWWGG) double digest, 10313 thousand fragments (7199434+3114107 sites), 288 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     186 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     274 bp
  30% of sequence in fragments shorter than     315 bp
  35% of sequence in fragments shorter than     357 bp
  40% of sequence in fragments shorter than     401 bp
  45% of sequence in fragments shorter than     447 bp
  50% of sequence in fragments shorter than     493 bp
  55% of sequence in fragments shorter than     546 bp
  60% of sequence in fragments shorter than     603 bp
  65% of sequence in fragments shorter than     666 bp
  70% of sequence in fragments shorter than     740 bp
  75% of sequence in fragments shorter than     826 bp
  80% of sequence in fragments shorter than     929 bp
  85% of sequence in fragments shorter than    1062 bp
  90% of sequence in fragments shorter than    1256 bp
  95% of sequence in fragments shorter than    1611 bp

# BspKT6I.NspI (GATC+RCATGY) double digest, 10303 thousand fragments (7199434+3104359 sites), 289 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     185 bp
  20% of sequence in fragments shorter than     229 bp
  25% of sequence in fragments shorter than     271 bp
  30% of sequence in fragments shorter than     312 bp
  35% of sequence in fragments shorter than     353 bp
  40% of sequence in fragments shorter than     396 bp
  45% of sequence in fragments shorter than     440 bp
  50% of sequence in fragments shorter than     485 bp
  55% of sequence in fragments shorter than     536 bp
  60% of sequence in fragments shorter than     591 bp
  65% of sequence in fragments shorter than     654 bp
  70% of sequence in fragments shorter than     724 bp
  75% of sequence in fragments shorter than     807 bp
  80% of sequence in fragments shorter than     906 bp
  85% of sequence in fragments shorter than    1031 bp
  90% of sequence in fragments shorter than    1205 bp
  95% of sequence in fragments shorter than    1526 bp

# BstNI.EcoRV (CCWGG+GATATC) double digest, 10301 thousand fragments (9855646+446473 sites), 289 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     128 bp
  15% of sequence in fragments shorter than     175 bp
  20% of sequence in fragments shorter than     229 bp
  25% of sequence in fragments shorter than     284 bp
  30% of sequence in fragments shorter than     340 bp
  35% of sequence in fragments shorter than     400 bp
  40% of sequence in fragments shorter than     466 bp
  45% of sequence in fragments shorter than     536 bp
  50% of sequence in fragments shorter than     612 bp
  55% of sequence in fragments shorter than     695 bp
  60% of sequence in fragments shorter than     790 bp
  65% of sequence in fragments shorter than     901 bp
  70% of sequence in fragments shorter than    1029 bp
  75% of sequence in fragments shorter than    1182 bp
  80% of sequence in fragments shorter than    1374 bp
  85% of sequence in fragments shorter than    1633 bp
  90% of sequence in fragments shorter than    2008 bp
  95% of sequence in fragments shorter than    2714 bp

# AccI.MboII (GTMKAC+GAAGA) double digest, 10300 thousand fragments (1101063+9199496 sites), 289 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     195 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     287 bp
  30% of sequence in fragments shorter than     331 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     425 bp
  45% of sequence in fragments shorter than     474 bp
  50% of sequence in fragments shorter than     525 bp
  55% of sequence in fragments shorter than     581 bp
  60% of sequence in fragments shorter than     644 bp
  65% of sequence in fragments shorter than     714 bp
  70% of sequence in fragments shorter than     788 bp
  75% of sequence in fragments shorter than     878 bp
  80% of sequence in fragments shorter than     988 bp
  85% of sequence in fragments shorter than    1120 bp
  90% of sequence in fragments shorter than    1314 bp
  95% of sequence in fragments shorter than    1647 bp

# MboII.PpuMI (GAAGA+RGGWCCY) double digest, 10284 thousand fragments (9199496+1085138 sites), 289 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     195 bp
  20% of sequence in fragments shorter than     242 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     378 bp
  40% of sequence in fragments shorter than     428 bp
  45% of sequence in fragments shorter than     478 bp
  50% of sequence in fragments shorter than     532 bp
  55% of sequence in fragments shorter than     592 bp
  60% of sequence in fragments shorter than     656 bp
  65% of sequence in fragments shorter than     727 bp
  70% of sequence in fragments shorter than     808 bp
  75% of sequence in fragments shorter than     899 bp
  80% of sequence in fragments shorter than    1010 bp
  85% of sequence in fragments shorter than    1146 bp
  90% of sequence in fragments shorter than    1344 bp
  95% of sequence in fragments shorter than    1681 bp

# BscAI.BsmAI (GCATC+GTCTC) double digest, 10284 thousand fragments (3652926+6631585 sites), 289 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     189 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     276 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     368 bp
  40% of sequence in fragments shorter than     415 bp
  45% of sequence in fragments shorter than     465 bp
  50% of sequence in fragments shorter than     518 bp
  55% of sequence in fragments shorter than     576 bp
  60% of sequence in fragments shorter than     640 bp
  65% of sequence in fragments shorter than     711 bp
  70% of sequence in fragments shorter than     792 bp
  75% of sequence in fragments shorter than     889 bp
  80% of sequence in fragments shorter than    1008 bp
  85% of sequence in fragments shorter than    1163 bp
  90% of sequence in fragments shorter than    1384 bp
  95% of sequence in fragments shorter than    1777 bp

# MboII.PvuII (GAAGA+CAGCTG) double digest, 10283 thousand fragments (9199496+1084260 sites), 289 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     196 bp
  20% of sequence in fragments shorter than     242 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     426 bp
  45% of sequence in fragments shorter than     476 bp
  50% of sequence in fragments shorter than     529 bp
  55% of sequence in fragments shorter than     587 bp
  60% of sequence in fragments shorter than     650 bp
  65% of sequence in fragments shorter than     721 bp
  70% of sequence in fragments shorter than     800 bp
  75% of sequence in fragments shorter than     890 bp
  80% of sequence in fragments shorter than     999 bp
  85% of sequence in fragments shorter than    1132 bp
  90% of sequence in fragments shorter than    1325 bp
  95% of sequence in fragments shorter than    1653 bp

# BbvI.TfiI (GCAGC+GAWTC) double digest, 10273 thousand fragments (5290051+4984362 sites), 290 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     245 bp
  25% of sequence in fragments shorter than     287 bp
  30% of sequence in fragments shorter than     330 bp
  35% of sequence in fragments shorter than     371 bp
  40% of sequence in fragments shorter than     419 bp
  45% of sequence in fragments shorter than     469 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     581 bp
  60% of sequence in fragments shorter than     644 bp
  65% of sequence in fragments shorter than     711 bp
  70% of sequence in fragments shorter than     786 bp
  75% of sequence in fragments shorter than     877 bp
  80% of sequence in fragments shorter than     988 bp
  85% of sequence in fragments shorter than    1127 bp
  90% of sequence in fragments shorter than    1321 bp
  95% of sequence in fragments shorter than    1652 bp

# BfiI.HaeIII (ACTGGG+GGCC) double digest, 10268 thousand fragments (1706594+8562230 sites), 290 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     147 bp
  15% of sequence in fragments shorter than     202 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     476 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     610 bp
  55% of sequence in fragments shorter than     689 bp
  60% of sequence in fragments shorter than     770 bp
  65% of sequence in fragments shorter than     864 bp
  70% of sequence in fragments shorter than     972 bp
  75% of sequence in fragments shorter than    1100 bp
  80% of sequence in fragments shorter than    1263 bp
  85% of sequence in fragments shorter than    1470 bp
  90% of sequence in fragments shorter than    1769 bp
  95% of sequence in fragments shorter than    2282 bp

# Hpy188I.PstI (TCNGA+CTGCAG) double digest, 10263 thousand fragments (8942153+1321469 sites), 290 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     188 bp
  20% of sequence in fragments shorter than     233 bp
  25% of sequence in fragments shorter than     276 bp
  30% of sequence in fragments shorter than     320 bp
  35% of sequence in fragments shorter than     359 bp
  40% of sequence in fragments shorter than     407 bp
  45% of sequence in fragments shorter than     455 bp
  50% of sequence in fragments shorter than     507 bp
  55% of sequence in fragments shorter than     563 bp
  60% of sequence in fragments shorter than     622 bp
  65% of sequence in fragments shorter than     690 bp
  70% of sequence in fragments shorter than     767 bp
  75% of sequence in fragments shorter than     854 bp
  80% of sequence in fragments shorter than     960 bp
  85% of sequence in fragments shorter than    1093 bp
  90% of sequence in fragments shorter than    1265 bp
  95% of sequence in fragments shorter than    1571 bp

# BstNI.SpeI (CCWGG+ACTAGT) double digest, 10255 thousand fragments (9855646+400286 sites), 290 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     176 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     285 bp
  30% of sequence in fragments shorter than     342 bp
  35% of sequence in fragments shorter than     402 bp
  40% of sequence in fragments shorter than     468 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     616 bp
  55% of sequence in fragments shorter than     699 bp
  60% of sequence in fragments shorter than     794 bp
  65% of sequence in fragments shorter than     905 bp
  70% of sequence in fragments shorter than    1033 bp
  75% of sequence in fragments shorter than    1188 bp
  80% of sequence in fragments shorter than    1380 bp
  85% of sequence in fragments shorter than    1619 bp
  90% of sequence in fragments shorter than    1989 bp
  95% of sequence in fragments shorter than    2704 bp

# AflIII.TspRI (ACRYGT+CASTG) double digest, 10250 thousand fragments (1485394+8765240 sites), 290 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     281 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     365 bp
  40% of sequence in fragments shorter than     408 bp
  45% of sequence in fragments shorter than     455 bp
  50% of sequence in fragments shorter than     505 bp
  55% of sequence in fragments shorter than     558 bp
  60% of sequence in fragments shorter than     618 bp
  65% of sequence in fragments shorter than     683 bp
  70% of sequence in fragments shorter than     758 bp
  75% of sequence in fragments shorter than     847 bp
  80% of sequence in fragments shorter than     950 bp
  85% of sequence in fragments shorter than    1084 bp
  90% of sequence in fragments shorter than    1278 bp
  95% of sequence in fragments shorter than    1617 bp

# BstNI.HpaI (CCWGG+GTTAAC) double digest, 10244 thousand fragments (9855646+389418 sites), 290 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     176 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     285 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     402 bp
  40% of sequence in fragments shorter than     468 bp
  45% of sequence in fragments shorter than     540 bp
  50% of sequence in fragments shorter than     617 bp
  55% of sequence in fragments shorter than     702 bp
  60% of sequence in fragments shorter than     799 bp
  65% of sequence in fragments shorter than     913 bp
  70% of sequence in fragments shorter than    1045 bp
  75% of sequence in fragments shorter than    1207 bp
  80% of sequence in fragments shorter than    1408 bp
  85% of sequence in fragments shorter than    1685 bp
  90% of sequence in fragments shorter than    2092 bp
  95% of sequence in fragments shorter than    2864 bp

# HinfI.PstI (GANTC+CTGCAG) double digest, 10237 thousand fragments (8916054+1321469 sites), 291 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     282 bp
  30% of sequence in fragments shorter than     322 bp
  35% of sequence in fragments shorter than     356 bp
  40% of sequence in fragments shorter than     398 bp
  45% of sequence in fragments shorter than     444 bp
  50% of sequence in fragments shorter than     492 bp
  55% of sequence in fragments shorter than     543 bp
  60% of sequence in fragments shorter than     600 bp
  65% of sequence in fragments shorter than     661 bp
  70% of sequence in fragments shorter than     727 bp
  75% of sequence in fragments shorter than     807 bp
  80% of sequence in fragments shorter than     904 bp
  85% of sequence in fragments shorter than    1027 bp
  90% of sequence in fragments shorter than    1197 bp
  95% of sequence in fragments shorter than    1485 bp

# BstNI.SapI (CCWGG+GCTCTTC) double digest, 10232 thousand fragments (9855646+377161 sites), 291 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     128 bp
  15% of sequence in fragments shorter than     176 bp
  20% of sequence in fragments shorter than     230 bp
  25% of sequence in fragments shorter than     286 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     475 bp
  45% of sequence in fragments shorter than     548 bp
  50% of sequence in fragments shorter than     627 bp
  55% of sequence in fragments shorter than     717 bp
  60% of sequence in fragments shorter than     817 bp
  65% of sequence in fragments shorter than     936 bp
  70% of sequence in fragments shorter than    1075 bp
  75% of sequence in fragments shorter than    1245 bp
  80% of sequence in fragments shorter than    1457 bp
  85% of sequence in fragments shorter than    1749 bp
  90% of sequence in fragments shorter than    2169 bp
  95% of sequence in fragments shorter than    2959 bp

# BstNI.SmaI (CCWGG+CCCGGG) double digest, 10232 thousand fragments (9855646+376940 sites), 291 bp average fragment length
   5% of sequence in fragments shorter than      78 bp
  10% of sequence in fragments shorter than     124 bp
  15% of sequence in fragments shorter than     176 bp
  20% of sequence in fragments shorter than     232 bp
  25% of sequence in fragments shorter than     291 bp
  30% of sequence in fragments shorter than     352 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     489 bp
  45% of sequence in fragments shorter than     568 bp
  50% of sequence in fragments shorter than     653 bp
  55% of sequence in fragments shorter than     745 bp
  60% of sequence in fragments shorter than     854 bp
  65% of sequence in fragments shorter than     981 bp
  70% of sequence in fragments shorter than    1127 bp
  75% of sequence in fragments shorter than    1306 bp
  80% of sequence in fragments shorter than    1534 bp
  85% of sequence in fragments shorter than    1842 bp
  90% of sequence in fragments shorter than    2278 bp
  95% of sequence in fragments shorter than    3136 bp

# BthCI.Tsp45I (GCNGC+GTSAC) double digest, 10231 thousand fragments (6209929+4021761 sites), 291 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     154 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     307 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     468 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     593 bp
  55% of sequence in fragments shorter than     663 bp
  60% of sequence in fragments shorter than     741 bp
  65% of sequence in fragments shorter than     828 bp
  70% of sequence in fragments shorter than     926 bp
  75% of sequence in fragments shorter than    1041 bp
  80% of sequence in fragments shorter than    1182 bp
  85% of sequence in fragments shorter than    1365 bp
  90% of sequence in fragments shorter than    1619 bp
  95% of sequence in fragments shorter than    2045 bp

# BamHI.BstNI (GGATCC+CCWGG) double digest, 10221 thousand fragments (365999+9855646 sites), 291 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     177 bp
  20% of sequence in fragments shorter than     233 bp
  25% of sequence in fragments shorter than     290 bp
  30% of sequence in fragments shorter than     348 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     556 bp
  50% of sequence in fragments shorter than     637 bp
  55% of sequence in fragments shorter than     726 bp
  60% of sequence in fragments shorter than     828 bp
  65% of sequence in fragments shorter than     944 bp
  70% of sequence in fragments shorter than    1081 bp
  75% of sequence in fragments shorter than    1249 bp
  80% of sequence in fragments shorter than    1455 bp
  85% of sequence in fragments shorter than    1733 bp
  90% of sequence in fragments shorter than    2129 bp
  95% of sequence in fragments shorter than    2933 bp

# AciI.HphI (CCGC+GGTGA) double digest, 10212 thousand fragments (4206130+6007336 sites), 291 bp average fragment length
   5% of sequence in fragments shorter than      92 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     377 bp
  35% of sequence in fragments shorter than     436 bp
  40% of sequence in fragments shorter than     498 bp
  45% of sequence in fragments shorter than     562 bp
  50% of sequence in fragments shorter than     629 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     787 bp
  65% of sequence in fragments shorter than     875 bp
  70% of sequence in fragments shorter than     981 bp
  75% of sequence in fragments shorter than    1104 bp
  80% of sequence in fragments shorter than    1253 bp
  85% of sequence in fragments shorter than    1437 bp
  90% of sequence in fragments shorter than    1706 bp
  95% of sequence in fragments shorter than    2162 bp

# HaeIII.PsiI (GGCC+TTATAA) double digest, 10209 thousand fragments (8562230+1647912 sites), 291 bp average fragment length
   5% of sequence in fragments shorter than      90 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     395 bp
  40% of sequence in fragments shorter than     446 bp
  45% of sequence in fragments shorter than     503 bp
  50% of sequence in fragments shorter than     560 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     693 bp
  65% of sequence in fragments shorter than     772 bp
  70% of sequence in fragments shorter than     860 bp
  75% of sequence in fragments shorter than     955 bp
  80% of sequence in fragments shorter than    1077 bp
  85% of sequence in fragments shorter than    1234 bp
  90% of sequence in fragments shorter than    1451 bp
  95% of sequence in fragments shorter than    1823 bp

# AvaI.MboII (CYCGRG+GAAGA) double digest, 10204 thousand fragments (1005714+9199496 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     289 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     422 bp
  45% of sequence in fragments shorter than     469 bp
  50% of sequence in fragments shorter than     520 bp
  55% of sequence in fragments shorter than     576 bp
  60% of sequence in fragments shorter than     636 bp
  65% of sequence in fragments shorter than     703 bp
  70% of sequence in fragments shorter than     778 bp
  75% of sequence in fragments shorter than     863 bp
  80% of sequence in fragments shorter than     967 bp
  85% of sequence in fragments shorter than    1090 bp
  90% of sequence in fragments shorter than    1268 bp
  95% of sequence in fragments shorter than    1567 bp

# FauI.TspRI (CCCGC+CASTG) double digest, 10204 thousand fragments (1439772+8765240 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     334 bp
  35% of sequence in fragments shorter than     382 bp
  40% of sequence in fragments shorter than     432 bp
  45% of sequence in fragments shorter than     485 bp
  50% of sequence in fragments shorter than     542 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     673 bp
  65% of sequence in fragments shorter than     744 bp
  70% of sequence in fragments shorter than     832 bp
  75% of sequence in fragments shorter than     933 bp
  80% of sequence in fragments shorter than    1051 bp
  85% of sequence in fragments shorter than    1207 bp
  90% of sequence in fragments shorter than    1426 bp
  95% of sequence in fragments shorter than    1809 bp

# BspMI.TspRI (ACCTGC+CASTG) double digest, 10202 thousand fragments (1438129+8765240 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     283 bp
  30% of sequence in fragments shorter than     327 bp
  35% of sequence in fragments shorter than     369 bp
  40% of sequence in fragments shorter than     416 bp
  45% of sequence in fragments shorter than     466 bp
  50% of sequence in fragments shorter than     519 bp
  55% of sequence in fragments shorter than     574 bp
  60% of sequence in fragments shorter than     638 bp
  65% of sequence in fragments shorter than     707 bp
  70% of sequence in fragments shorter than     788 bp
  75% of sequence in fragments shorter than     880 bp
  80% of sequence in fragments shorter than     989 bp
  85% of sequence in fragments shorter than    1130 bp
  90% of sequence in fragments shorter than    1328 bp
  95% of sequence in fragments shorter than    1654 bp

# BccI.Tsp45I (CCATC+GTSAC) double digest, 10192 thousand fragments (6170927+4021761 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     154 bp
  15% of sequence in fragments shorter than     195 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     283 bp
  30% of sequence in fragments shorter than     326 bp
  35% of sequence in fragments shorter than     367 bp
  40% of sequence in fragments shorter than     413 bp
  45% of sequence in fragments shorter than     461 bp
  50% of sequence in fragments shorter than     513 bp
  55% of sequence in fragments shorter than     569 bp
  60% of sequence in fragments shorter than     629 bp
  65% of sequence in fragments shorter than     695 bp
  70% of sequence in fragments shorter than     773 bp
  75% of sequence in fragments shorter than     862 bp
  80% of sequence in fragments shorter than     973 bp
  85% of sequence in fragments shorter than    1111 bp
  90% of sequence in fragments shorter than    1315 bp
  95% of sequence in fragments shorter than    1670 bp

# BstEII.BstNI (GGTNACC+CCWGG) double digest, 10191 thousand fragments (335865+9855646 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     177 bp
  20% of sequence in fragments shorter than     232 bp
  25% of sequence in fragments shorter than     289 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     557 bp
  50% of sequence in fragments shorter than     637 bp
  55% of sequence in fragments shorter than     726 bp
  60% of sequence in fragments shorter than     830 bp
  65% of sequence in fragments shorter than     951 bp
  70% of sequence in fragments shorter than    1092 bp
  75% of sequence in fragments shorter than    1264 bp
  80% of sequence in fragments shorter than    1481 bp
  85% of sequence in fragments shorter than    1775 bp
  90% of sequence in fragments shorter than    2203 bp
  95% of sequence in fragments shorter than    3028 bp

# FokI.TfiI (GGATG+GAWTC) double digest, 10184 thousand fragments (5201116+4984362 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     243 bp
  25% of sequence in fragments shorter than     283 bp
  30% of sequence in fragments shorter than     324 bp
  35% of sequence in fragments shorter than     359 bp
  40% of sequence in fragments shorter than     403 bp
  45% of sequence in fragments shorter than     450 bp
  50% of sequence in fragments shorter than     498 bp
  55% of sequence in fragments shorter than     552 bp
  60% of sequence in fragments shorter than     608 bp
  65% of sequence in fragments shorter than     671 bp
  70% of sequence in fragments shorter than     740 bp
  75% of sequence in fragments shorter than     823 bp
  80% of sequence in fragments shorter than     922 bp
  85% of sequence in fragments shorter than    1047 bp
  90% of sequence in fragments shorter than    1219 bp
  95% of sequence in fragments shorter than    1509 bp

# BspHI.MboII (TCATGA+GAAGA) double digest, 10177 thousand fragments (978290+9199496 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     199 bp
  20% of sequence in fragments shorter than     247 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     338 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     432 bp
  45% of sequence in fragments shorter than     484 bp
  50% of sequence in fragments shorter than     537 bp
  55% of sequence in fragments shorter than     596 bp
  60% of sequence in fragments shorter than     660 bp
  65% of sequence in fragments shorter than     731 bp
  70% of sequence in fragments shorter than     812 bp
  75% of sequence in fragments shorter than     904 bp
  80% of sequence in fragments shorter than    1011 bp
  85% of sequence in fragments shorter than    1152 bp
  90% of sequence in fragments shorter than    1350 bp
  95% of sequence in fragments shorter than    1689 bp

# Hin4II.StyI (CCTTC+CCWWGG) double digest, 10173 thousand fragments (7059915+3114107 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     389 bp
  40% of sequence in fragments shorter than     438 bp
  45% of sequence in fragments shorter than     490 bp
  50% of sequence in fragments shorter than     546 bp
  55% of sequence in fragments shorter than     609 bp
  60% of sequence in fragments shorter than     678 bp
  65% of sequence in fragments shorter than     752 bp
  70% of sequence in fragments shorter than     837 bp
  75% of sequence in fragments shorter than     935 bp
  80% of sequence in fragments shorter than    1056 bp
  85% of sequence in fragments shorter than    1211 bp
  90% of sequence in fragments shorter than    1429 bp
  95% of sequence in fragments shorter than    1799 bp

# BscGI.Hpy188I (CCCGT+TCNGA) double digest, 10172 thousand fragments (1231070+8942153 sites), 292 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     193 bp
  20% of sequence in fragments shorter than     235 bp
  25% of sequence in fragments shorter than     279 bp
  30% of sequence in fragments shorter than     323 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     411 bp
  45% of sequence in fragments shorter than     459 bp
  50% of sequence in fragments shorter than     512 bp
  55% of sequence in fragments shorter than     568 bp
  60% of sequence in fragments shorter than     627 bp
  65% of sequence in fragments shorter than     695 bp
  70% of sequence in fragments shorter than     771 bp
  75% of sequence in fragments shorter than     858 bp
  80% of sequence in fragments shorter than     963 bp
  85% of sequence in fragments shorter than    1094 bp
  90% of sequence in fragments shorter than    1265 bp
  95% of sequence in fragments shorter than    1567 bp

# EsaBC3I.HaeIII (TCGA+GGCC) double digest, 10165 thousand fragments (1603346+8562230 sites), 293 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     364 bp
  35% of sequence in fragments shorter than     423 bp
  40% of sequence in fragments shorter than     484 bp
  45% of sequence in fragments shorter than     548 bp
  50% of sequence in fragments shorter than     619 bp
  55% of sequence in fragments shorter than     694 bp
  60% of sequence in fragments shorter than     782 bp
  65% of sequence in fragments shorter than     873 bp
  70% of sequence in fragments shorter than     986 bp
  75% of sequence in fragments shorter than    1115 bp
  80% of sequence in fragments shorter than    1272 bp
  85% of sequence in fragments shorter than    1477 bp
  90% of sequence in fragments shorter than    1766 bp
  95% of sequence in fragments shorter than    2264 bp

# Hin4II.NspI (CCTTC+RCATGY) double digest, 10163 thousand fragments (7059915+3104359 sites), 293 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     158 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     339 bp
  35% of sequence in fragments shorter than     380 bp
  40% of sequence in fragments shorter than     428 bp
  45% of sequence in fragments shorter than     479 bp
  50% of sequence in fragments shorter than     529 bp
  55% of sequence in fragments shorter than     586 bp
  60% of sequence in fragments shorter than     650 bp
  65% of sequence in fragments shorter than     719 bp
  70% of sequence in fragments shorter than     794 bp
  75% of sequence in fragments shorter than     884 bp
  80% of sequence in fragments shorter than     994 bp
  85% of sequence in fragments shorter than    1131 bp
  90% of sequence in fragments shorter than    1324 bp
  95% of sequence in fragments shorter than    1650 bp

# BsmAI.SfeI (GTCTC+CTRYAG) double digest, 10162 thousand fragments (6631585+3531009 sites), 293 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     149 bp
  15% of sequence in fragments shorter than     194 bp
  20% of sequence in fragments shorter than     245 bp
  25% of sequence in fragments shorter than     294 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     445 bp
  45% of sequence in fragments shorter than     496 bp
  50% of sequence in fragments shorter than     550 bp
  55% of sequence in fragments shorter than     612 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     755 bp
  70% of sequence in fragments shorter than     843 bp
  75% of sequence in fragments shorter than     946 bp
  80% of sequence in fragments shorter than    1067 bp
  85% of sequence in fragments shorter than    1224 bp
  90% of sequence in fragments shorter than    1439 bp
  95% of sequence in fragments shorter than    1829 bp

# BstNI.SexAI (CCWGG+ACCWGGT) double digest, 10153 thousand fragments (9855646+297900 sites), 293 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     183 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     361 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     499 bp
  45% of sequence in fragments shorter than     578 bp
  50% of sequence in fragments shorter than     661 bp
  55% of sequence in fragments shorter than     755 bp
  60% of sequence in fragments shorter than     865 bp
  65% of sequence in fragments shorter than     992 bp
  70% of sequence in fragments shorter than    1139 bp
  75% of sequence in fragments shorter than    1319 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1856 bp
  90% of sequence in fragments shorter than    2295 bp
  95% of sequence in fragments shorter than    3158 bp

# BscGI.HinfI (CCCGT+GANTC) double digest, 10146 thousand fragments (1231070+8916054 sites), 293 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     150 bp
  15% of sequence in fragments shorter than     193 bp
  20% of sequence in fragments shorter than     235 bp
  25% of sequence in fragments shorter than     280 bp
  30% of sequence in fragments shorter than     319 bp
  35% of sequence in fragments shorter than     359 bp
  40% of sequence in fragments shorter than     403 bp
  45% of sequence in fragments shorter than     451 bp
  50% of sequence in fragments shorter than     501 bp
  55% of sequence in fragments shorter than     555 bp
  60% of sequence in fragments shorter than     614 bp
  65% of sequence in fragments shorter than     678 bp
  70% of sequence in fragments shorter than     749 bp
  75% of sequence in fragments shorter than     832 bp
  80% of sequence in fragments shorter than     934 bp
  85% of sequence in fragments shorter than    1061 bp
  90% of sequence in fragments shorter than    1239 bp
  95% of sequence in fragments shorter than    1538 bp

# BstNI.KpnI (CCWGG+GGTACC) double digest, 10143 thousand fragments (9855646+287974 sites), 293 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     130 bp
  15% of sequence in fragments shorter than     178 bp
  20% of sequence in fragments shorter than     234 bp
  25% of sequence in fragments shorter than     291 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     414 bp
  40% of sequence in fragments shorter than     483 bp
  45% of sequence in fragments shorter than     559 bp
  50% of sequence in fragments shorter than     639 bp
  55% of sequence in fragments shorter than     725 bp
  60% of sequence in fragments shorter than     829 bp
  65% of sequence in fragments shorter than     950 bp
  70% of sequence in fragments shorter than    1090 bp
  75% of sequence in fragments shorter than    1256 bp
  80% of sequence in fragments shorter than    1467 bp
  85% of sequence in fragments shorter than    1747 bp
  90% of sequence in fragments shorter than    2159 bp
  95% of sequence in fragments shorter than    2977 bp

# BstNI.NheI (CCWGG+GCTAGC) double digest, 10142 thousand fragments (9855646+287384 sites), 293 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     177 bp
  20% of sequence in fragments shorter than     233 bp
  25% of sequence in fragments shorter than     290 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     554 bp
  50% of sequence in fragments shorter than     637 bp
  55% of sequence in fragments shorter than     725 bp
  60% of sequence in fragments shorter than     821 bp
  65% of sequence in fragments shorter than     941 bp
  70% of sequence in fragments shorter than    1075 bp
  75% of sequence in fragments shorter than    1238 bp
  80% of sequence in fragments shorter than    1442 bp
  85% of sequence in fragments shorter than    1715 bp
  90% of sequence in fragments shorter than    2105 bp
  95% of sequence in fragments shorter than    2905 bp

# BthCI.MlyI (GCNGC+GAGTC) double digest, 10141 thousand fragments (6209929+3931692 sites), 293 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     154 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     262 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     361 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     472 bp
  45% of sequence in fragments shorter than     532 bp
  50% of sequence in fragments shorter than     596 bp
  55% of sequence in fragments shorter than     666 bp
  60% of sequence in fragments shorter than     742 bp
  65% of sequence in fragments shorter than     828 bp
  70% of sequence in fragments shorter than     925 bp
  75% of sequence in fragments shorter than    1037 bp
  80% of sequence in fragments shorter than    1177 bp
  85% of sequence in fragments shorter than    1353 bp
  90% of sequence in fragments shorter than    1603 bp
  95% of sequence in fragments shorter than    2034 bp

# AvaIII.MboII (ATGCAT+GAAGA) double digest, 10132 thousand fragments (933357+9199496 sites), 294 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     199 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     338 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     432 bp
  45% of sequence in fragments shorter than     483 bp
  50% of sequence in fragments shorter than     537 bp
  55% of sequence in fragments shorter than     595 bp
  60% of sequence in fragments shorter than     659 bp
  65% of sequence in fragments shorter than     731 bp
  70% of sequence in fragments shorter than     811 bp
  75% of sequence in fragments shorter than     903 bp
  80% of sequence in fragments shorter than    1014 bp
  85% of sequence in fragments shorter than    1151 bp
  90% of sequence in fragments shorter than    1350 bp
  95% of sequence in fragments shorter than    1693 bp

# MboII.StuI (GAAGA+AGGCCT) double digest, 10124 thousand fragments (9199496+925729 sites), 294 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     248 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     340 bp
  35% of sequence in fragments shorter than     388 bp
  40% of sequence in fragments shorter than     438 bp
  45% of sequence in fragments shorter than     489 bp
  50% of sequence in fragments shorter than     544 bp
  55% of sequence in fragments shorter than     604 bp
  60% of sequence in fragments shorter than     670 bp
  65% of sequence in fragments shorter than     743 bp
  70% of sequence in fragments shorter than     825 bp
  75% of sequence in fragments shorter than     918 bp
  80% of sequence in fragments shorter than    1030 bp
  85% of sequence in fragments shorter than    1174 bp
  90% of sequence in fragments shorter than    1375 bp
  95% of sequence in fragments shorter than    1717 bp

# HaeIII.HgiCI (GGCC+GGYRCC) double digest, 10112 thousand fragments (8562230+1550877 sites), 294 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     144 bp
  15% of sequence in fragments shorter than     199 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     495 bp
  45% of sequence in fragments shorter than     563 bp
  50% of sequence in fragments shorter than     637 bp
  55% of sequence in fragments shorter than     718 bp
  60% of sequence in fragments shorter than     813 bp
  65% of sequence in fragments shorter than     916 bp
  70% of sequence in fragments shorter than    1038 bp
  75% of sequence in fragments shorter than    1183 bp
  80% of sequence in fragments shorter than    1350 bp
  85% of sequence in fragments shorter than    1576 bp
  90% of sequence in fragments shorter than    1896 bp
  95% of sequence in fragments shorter than    2462 bp

# BsmI.HaeIII (GAATGC+GGCC) double digest, 10111 thousand fragments (1549350+8562230 sites), 294 bp average fragment length
   5% of sequence in fragments shorter than      88 bp
  10% of sequence in fragments shorter than     145 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     252 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     357 bp
  35% of sequence in fragments shorter than     416 bp
  40% of sequence in fragments shorter than     476 bp
  45% of sequence in fragments shorter than     540 bp
  50% of sequence in fragments shorter than     608 bp
  55% of sequence in fragments shorter than     685 bp
  60% of sequence in fragments shorter than     770 bp
  65% of sequence in fragments shorter than     863 bp
  70% of sequence in fragments shorter than     969 bp
  75% of sequence in fragments shorter than    1097 bp
  80% of sequence in fragments shorter than    1252 bp
  85% of sequence in fragments shorter than    1446 bp
  90% of sequence in fragments shorter than    1724 bp
  95% of sequence in fragments shorter than    2205 bp

# MboII.NdeI (GAAGA+CATATG) double digest, 10102 thousand fragments (9199496+903360 sites), 294 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     248 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     338 bp
  35% of sequence in fragments shorter than     383 bp
  40% of sequence in fragments shorter than     432 bp
  45% of sequence in fragments shorter than     485 bp
  50% of sequence in fragments shorter than     539 bp
  55% of sequence in fragments shorter than     597 bp
  60% of sequence in fragments shorter than     661 bp
  65% of sequence in fragments shorter than     733 bp
  70% of sequence in fragments shorter than     811 bp
  75% of sequence in fragments shorter than     904 bp
  80% of sequence in fragments shorter than    1017 bp
  85% of sequence in fragments shorter than    1156 bp
  90% of sequence in fragments shorter than    1358 bp
  95% of sequence in fragments shorter than    1706 bp

# BccI.MlyI (CCATC+GAGTC) double digest, 10102 thousand fragments (6170927+3931692 sites), 294 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     195 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     285 bp
  30% of sequence in fragments shorter than     328 bp
  35% of sequence in fragments shorter than     373 bp
  40% of sequence in fragments shorter than     420 bp
  45% of sequence in fragments shorter than     469 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     581 bp
  60% of sequence in fragments shorter than     644 bp
  65% of sequence in fragments shorter than     715 bp
  70% of sequence in fragments shorter than     796 bp
  75% of sequence in fragments shorter than     888 bp
  80% of sequence in fragments shorter than    1006 bp
  85% of sequence in fragments shorter than    1157 bp
  90% of sequence in fragments shorter than    1377 bp
  95% of sequence in fragments shorter than    1786 bp

# CviQI.TfiI (GTAC+GAWTC) double digest, 10098 thousand fragments (5115078+4984362 sites), 295 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     283 bp
  30% of sequence in fragments shorter than     324 bp
  35% of sequence in fragments shorter than     359 bp
  40% of sequence in fragments shorter than     403 bp
  45% of sequence in fragments shorter than     449 bp
  50% of sequence in fragments shorter than     497 bp
  55% of sequence in fragments shorter than     546 bp
  60% of sequence in fragments shorter than     603 bp
  65% of sequence in fragments shorter than     666 bp
  70% of sequence in fragments shorter than     734 bp
  75% of sequence in fragments shorter than     814 bp
  80% of sequence in fragments shorter than     910 bp
  85% of sequence in fragments shorter than    1032 bp
  90% of sequence in fragments shorter than    1202 bp
  95% of sequence in fragments shorter than    1492 bp

# BsrBI.BstNI (CCGCTC+CCWGG) double digest, 10090 thousand fragments (235523+9855646 sites), 295 bp average fragment length
   5% of sequence in fragments shorter than      83 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     178 bp
  20% of sequence in fragments shorter than     235 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     352 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     488 bp
  45% of sequence in fragments shorter than     566 bp
  50% of sequence in fragments shorter than     651 bp
  55% of sequence in fragments shorter than     742 bp
  60% of sequence in fragments shorter than     850 bp
  65% of sequence in fragments shorter than     976 bp
  70% of sequence in fragments shorter than    1121 bp
  75% of sequence in fragments shorter than    1298 bp
  80% of sequence in fragments shorter than    1524 bp
  85% of sequence in fragments shorter than    1829 bp
  90% of sequence in fragments shorter than    2252 bp
  95% of sequence in fragments shorter than    3098 bp

# PstI.TspRI (CTGCAG+CASTG) double digest, 10086 thousand fragments (1321469+8765240 sites), 295 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     251 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     387 bp
  40% of sequence in fragments shorter than     436 bp
  45% of sequence in fragments shorter than     486 bp
  50% of sequence in fragments shorter than     541 bp
  55% of sequence in fragments shorter than     601 bp
  60% of sequence in fragments shorter than     666 bp
  65% of sequence in fragments shorter than     738 bp
  70% of sequence in fragments shorter than     821 bp
  75% of sequence in fragments shorter than     920 bp
  80% of sequence in fragments shorter than    1033 bp
  85% of sequence in fragments shorter than    1181 bp
  90% of sequence in fragments shorter than    1390 bp
  95% of sequence in fragments shorter than    1758 bp

# BstNI.KasI (CCWGG+GGCGCC) double digest, 10085 thousand fragments (9855646+230381 sites), 295 bp average fragment length
   5% of sequence in fragments shorter than      82 bp
  10% of sequence in fragments shorter than     129 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     237 bp
  25% of sequence in fragments shorter than     295 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     422 bp
  40% of sequence in fragments shorter than     494 bp
  45% of sequence in fragments shorter than     573 bp
  50% of sequence in fragments shorter than     657 bp
  55% of sequence in fragments shorter than     750 bp
  60% of sequence in fragments shorter than     859 bp
  65% of sequence in fragments shorter than     986 bp
  70% of sequence in fragments shorter than    1134 bp
  75% of sequence in fragments shorter than    1313 bp
  80% of sequence in fragments shorter than    1542 bp
  85% of sequence in fragments shorter than    1850 bp
  90% of sequence in fragments shorter than    2288 bp
  95% of sequence in fragments shorter than    3146 bp

# BstNI.SwaI (CCWGG+ATTTAAAT) double digest, 10081 thousand fragments (9855646+226602 sites), 295 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     235 bp
  25% of sequence in fragments shorter than     291 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     479 bp
  45% of sequence in fragments shorter than     552 bp
  50% of sequence in fragments shorter than     631 bp
  55% of sequence in fragments shorter than     718 bp
  60% of sequence in fragments shorter than     817 bp
  65% of sequence in fragments shorter than     933 bp
  70% of sequence in fragments shorter than    1068 bp
  75% of sequence in fragments shorter than    1232 bp
  80% of sequence in fragments shorter than    1436 bp
  85% of sequence in fragments shorter than    1715 bp
  90% of sequence in fragments shorter than    2124 bp
  95% of sequence in fragments shorter than    2907 bp

# HindIII.MboII (AAGCTT+GAAGA) double digest, 10059 thousand fragments (860365+9199496 sites), 296 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     248 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     342 bp
  35% of sequence in fragments shorter than     387 bp
  40% of sequence in fragments shorter than     436 bp
  45% of sequence in fragments shorter than     490 bp
  50% of sequence in fragments shorter than     545 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     671 bp
  65% of sequence in fragments shorter than     741 bp
  70% of sequence in fragments shorter than     824 bp
  75% of sequence in fragments shorter than     918 bp
  80% of sequence in fragments shorter than    1031 bp
  85% of sequence in fragments shorter than    1176 bp
  90% of sequence in fragments shorter than    1380 bp
  95% of sequence in fragments shorter than    1729 bp

# MboII.XbaI (GAAGA+TCTAGA) double digest, 10049 thousand fragments (9199496+850998 sites), 296 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     158 bp
  15% of sequence in fragments shorter than     202 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     441 bp
  45% of sequence in fragments shorter than     495 bp
  50% of sequence in fragments shorter than     550 bp
  55% of sequence in fragments shorter than     610 bp
  60% of sequence in fragments shorter than     675 bp
  65% of sequence in fragments shorter than     748 bp
  70% of sequence in fragments shorter than     828 bp
  75% of sequence in fragments shorter than     922 bp
  80% of sequence in fragments shorter than    1035 bp
  85% of sequence in fragments shorter than    1181 bp
  90% of sequence in fragments shorter than    1387 bp
  95% of sequence in fragments shorter than    1737 bp

# AflIII.HaeIII (ACRYGT+GGCC) double digest, 10047 thousand fragments (1485394+8562230 sites), 296 bp average fragment length
   5% of sequence in fragments shorter than      90 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     410 bp
  40% of sequence in fragments shorter than     466 bp
  45% of sequence in fragments shorter than     525 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     664 bp
  60% of sequence in fragments shorter than     741 bp
  65% of sequence in fragments shorter than     831 bp
  70% of sequence in fragments shorter than     926 bp
  75% of sequence in fragments shorter than    1043 bp
  80% of sequence in fragments shorter than    1188 bp
  85% of sequence in fragments shorter than    1366 bp
  90% of sequence in fragments shorter than    1627 bp
  95% of sequence in fragments shorter than    2067 bp

# EcoRI.MboII (GAATTC+GAAGA) double digest, 10046 thousand fragments (847401+9199496 sites), 296 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     441 bp
  45% of sequence in fragments shorter than     495 bp
  50% of sequence in fragments shorter than     550 bp
  55% of sequence in fragments shorter than     610 bp
  60% of sequence in fragments shorter than     676 bp
  65% of sequence in fragments shorter than     749 bp
  70% of sequence in fragments shorter than     832 bp
  75% of sequence in fragments shorter than     923 bp
  80% of sequence in fragments shorter than    1036 bp
  85% of sequence in fragments shorter than    1181 bp
  90% of sequence in fragments shorter than    1386 bp
  95% of sequence in fragments shorter than    1734 bp

# AccI.Hpy188I (GTMKAC+TCNGA) double digest, 10042 thousand fragments (1101063+8942153 sites), 296 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     193 bp
  20% of sequence in fragments shorter than     238 bp
  25% of sequence in fragments shorter than     283 bp
  30% of sequence in fragments shorter than     327 bp
  35% of sequence in fragments shorter than     368 bp
  40% of sequence in fragments shorter than     415 bp
  45% of sequence in fragments shorter than     464 bp
  50% of sequence in fragments shorter than     516 bp
  55% of sequence in fragments shorter than     572 bp
  60% of sequence in fragments shorter than     632 bp
  65% of sequence in fragments shorter than     695 bp
  70% of sequence in fragments shorter than     772 bp
  75% of sequence in fragments shorter than     858 bp
  80% of sequence in fragments shorter than     963 bp
  85% of sequence in fragments shorter than    1096 bp
  90% of sequence in fragments shorter than    1270 bp
  95% of sequence in fragments shorter than    1579 bp

# ApoI.BscAI (RAATTY+GCATC) double digest, 10034 thousand fragments (6382433+3652926 sites), 296 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     152 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     242 bp
  25% of sequence in fragments shorter than     285 bp
  30% of sequence in fragments shorter than     329 bp
  35% of sequence in fragments shorter than     375 bp
  40% of sequence in fragments shorter than     423 bp
  45% of sequence in fragments shorter than     474 bp
  50% of sequence in fragments shorter than     524 bp
  55% of sequence in fragments shorter than     580 bp
  60% of sequence in fragments shorter than     641 bp
  65% of sequence in fragments shorter than     709 bp
  70% of sequence in fragments shorter than     788 bp
  75% of sequence in fragments shorter than     879 bp
  80% of sequence in fragments shorter than     985 bp
  85% of sequence in fragments shorter than    1127 bp
  90% of sequence in fragments shorter than    1324 bp
  95% of sequence in fragments shorter than    1668 bp

# AvaII.BspNCI (GGWCC+CCAGA) double digest, 10029 thousand fragments (2747690+7282436 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     252 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     346 bp
  35% of sequence in fragments shorter than     397 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     508 bp
  50% of sequence in fragments shorter than     566 bp
  55% of sequence in fragments shorter than     626 bp
  60% of sequence in fragments shorter than     697 bp
  65% of sequence in fragments shorter than     775 bp
  70% of sequence in fragments shorter than     860 bp
  75% of sequence in fragments shorter than     964 bp
  80% of sequence in fragments shorter than    1090 bp
  85% of sequence in fragments shorter than    1251 bp
  90% of sequence in fragments shorter than    1482 bp
  95% of sequence in fragments shorter than    1881 bp

# HphI.Tsp45I (GGTGA+GTSAC) double digest, 10028 thousand fragments (6007336+4021761 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than     116 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     272 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     367 bp
  35% of sequence in fragments shorter than     418 bp
  40% of sequence in fragments shorter than     469 bp
  45% of sequence in fragments shorter than     523 bp
  50% of sequence in fragments shorter than     580 bp
  55% of sequence in fragments shorter than     641 bp
  60% of sequence in fragments shorter than     709 bp
  65% of sequence in fragments shorter than     786 bp
  70% of sequence in fragments shorter than     866 bp
  75% of sequence in fragments shorter than     968 bp
  80% of sequence in fragments shorter than    1093 bp
  85% of sequence in fragments shorter than    1252 bp
  90% of sequence in fragments shorter than    1467 bp
  95% of sequence in fragments shorter than    1872 bp

# AclI.BstNI (AACGTT+CCWGG) double digest, 10027 thousand fragments (172706+9855646 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     236 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     352 bp
  35% of sequence in fragments shorter than     416 bp
  40% of sequence in fragments shorter than     486 bp
  45% of sequence in fragments shorter than     562 bp
  50% of sequence in fragments shorter than     643 bp
  55% of sequence in fragments shorter than     732 bp
  60% of sequence in fragments shorter than     836 bp
  65% of sequence in fragments shorter than     955 bp
  70% of sequence in fragments shorter than    1091 bp
  75% of sequence in fragments shorter than    1259 bp
  80% of sequence in fragments shorter than    1470 bp
  85% of sequence in fragments shorter than    1749 bp
  90% of sequence in fragments shorter than    2150 bp
  95% of sequence in fragments shorter than    2922 bp

# Hpy188I.PpuMI (TCNGA+RGGWCCY) double digest, 10026 thousand fragments (8942153+1085138 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     193 bp
  20% of sequence in fragments shorter than     238 bp
  25% of sequence in fragments shorter than     284 bp
  30% of sequence in fragments shorter than     330 bp
  35% of sequence in fragments shorter than     372 bp
  40% of sequence in fragments shorter than     420 bp
  45% of sequence in fragments shorter than     470 bp
  50% of sequence in fragments shorter than     526 bp
  55% of sequence in fragments shorter than     583 bp
  60% of sequence in fragments shorter than     648 bp
  65% of sequence in fragments shorter than     719 bp
  70% of sequence in fragments shorter than     800 bp
  75% of sequence in fragments shorter than     888 bp
  80% of sequence in fragments shorter than     998 bp
  85% of sequence in fragments shorter than    1138 bp
  90% of sequence in fragments shorter than    1331 bp
  95% of sequence in fragments shorter than    1663 bp

# Hpy188I.PvuII (TCNGA+CAGCTG) double digest, 10025 thousand fragments (8942153+1084260 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     153 bp
  15% of sequence in fragments shorter than     193 bp
  20% of sequence in fragments shorter than     238 bp
  25% of sequence in fragments shorter than     283 bp
  30% of sequence in fragments shorter than     328 bp
  35% of sequence in fragments shorter than     370 bp
  40% of sequence in fragments shorter than     418 bp
  45% of sequence in fragments shorter than     468 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     580 bp
  60% of sequence in fragments shorter than     642 bp
  65% of sequence in fragments shorter than     713 bp
  70% of sequence in fragments shorter than     793 bp
  75% of sequence in fragments shorter than     884 bp
  80% of sequence in fragments shorter than     994 bp
  85% of sequence in fragments shorter than    1133 bp
  90% of sequence in fragments shorter than    1312 bp
  95% of sequence in fragments shorter than    1634 bp

# AccI.HinfI (GTMKAC+GANTC) double digest, 10016 thousand fragments (1101063+8916054 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     202 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     327 bp
  35% of sequence in fragments shorter than     362 bp
  40% of sequence in fragments shorter than     404 bp
  45% of sequence in fragments shorter than     450 bp
  50% of sequence in fragments shorter than     497 bp
  55% of sequence in fragments shorter than     545 bp
  60% of sequence in fragments shorter than     602 bp
  65% of sequence in fragments shorter than     664 bp
  70% of sequence in fragments shorter than     729 bp
  75% of sequence in fragments shorter than     808 bp
  80% of sequence in fragments shorter than     902 bp
  85% of sequence in fragments shorter than    1021 bp
  90% of sequence in fragments shorter than    1184 bp
  95% of sequence in fragments shorter than    1459 bp

# BstNI.PacI (CCWGG+TTAATTAA) double digest, 10014 thousand fragments (9855646+159455 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     236 bp
  25% of sequence in fragments shorter than     294 bp
  30% of sequence in fragments shorter than     353 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     486 bp
  45% of sequence in fragments shorter than     562 bp
  50% of sequence in fragments shorter than     643 bp
  55% of sequence in fragments shorter than     731 bp
  60% of sequence in fragments shorter than     835 bp
  65% of sequence in fragments shorter than     955 bp
  70% of sequence in fragments shorter than    1095 bp
  75% of sequence in fragments shorter than    1264 bp
  80% of sequence in fragments shorter than    1477 bp
  85% of sequence in fragments shorter than    1768 bp
  90% of sequence in fragments shorter than    2187 bp
  95% of sequence in fragments shorter than    2993 bp

# BstNI.Hpy99I (CCWGG+CGWCG) double digest, 10013 thousand fragments (9855646+157917 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     179 bp
  20% of sequence in fragments shorter than     236 bp
  25% of sequence in fragments shorter than     295 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     420 bp
  40% of sequence in fragments shorter than     492 bp
  45% of sequence in fragments shorter than     570 bp
  50% of sequence in fragments shorter than     654 bp
  55% of sequence in fragments shorter than     746 bp
  60% of sequence in fragments shorter than     854 bp
  65% of sequence in fragments shorter than     981 bp
  70% of sequence in fragments shorter than    1126 bp
  75% of sequence in fragments shorter than    1304 bp
  80% of sequence in fragments shorter than    1531 bp
  85% of sequence in fragments shorter than    1838 bp
  90% of sequence in fragments shorter than    2270 bp
  95% of sequence in fragments shorter than    3120 bp

# BspNCI.SmlI (CCAGA+CTYRAG) double digest, 10009 thousand fragments (7282436+2727867 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     244 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     333 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     423 bp
  45% of sequence in fragments shorter than     473 bp
  50% of sequence in fragments shorter than     524 bp
  55% of sequence in fragments shorter than     579 bp
  60% of sequence in fragments shorter than     636 bp
  65% of sequence in fragments shorter than     703 bp
  70% of sequence in fragments shorter than     779 bp
  75% of sequence in fragments shorter than     866 bp
  80% of sequence in fragments shorter than     976 bp
  85% of sequence in fragments shorter than    1109 bp
  90% of sequence in fragments shorter than    1302 bp
  95% of sequence in fragments shorter than    1639 bp

# FauI.HaeIII (CCCGC+GGCC) double digest, 10001 thousand fragments (1439772+8562230 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than      87 bp
  10% of sequence in fragments shorter than     152 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     278 bp
  25% of sequence in fragments shorter than     341 bp
  30% of sequence in fragments shorter than     395 bp
  35% of sequence in fragments shorter than     461 bp
  40% of sequence in fragments shorter than     530 bp
  45% of sequence in fragments shorter than     607 bp
  50% of sequence in fragments shorter than     690 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     877 bp
  65% of sequence in fragments shorter than     992 bp
  70% of sequence in fragments shorter than    1123 bp
  75% of sequence in fragments shorter than    1272 bp
  80% of sequence in fragments shorter than    1462 bp
  85% of sequence in fragments shorter than    1710 bp
  90% of sequence in fragments shorter than    2060 bp
  95% of sequence in fragments shorter than    2675 bp

# HinfI.PpuMI (GANTC+RGGWCCY) double digest, 10000 thousand fragments (8916054+1085138 sites), 297 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     202 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     289 bp
  30% of sequence in fragments shorter than     328 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     407 bp
  45% of sequence in fragments shorter than     454 bp
  50% of sequence in fragments shorter than     504 bp
  55% of sequence in fragments shorter than     557 bp
  60% of sequence in fragments shorter than     616 bp
  65% of sequence in fragments shorter than     679 bp
  70% of sequence in fragments shorter than     747 bp
  75% of sequence in fragments shorter than     830 bp
  80% of sequence in fragments shorter than     930 bp
  85% of sequence in fragments shorter than    1055 bp
  90% of sequence in fragments shorter than    1232 bp
  95% of sequence in fragments shorter than    1530 bp

# BspMI.HaeIII (ACCTGC+GGCC) double digest, 9999 thousand fragments (1438129+8562230 sites), 298 bp average fragment length
   5% of sequence in fragments shorter than      88 bp
  10% of sequence in fragments shorter than     149 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     432 bp
  40% of sequence in fragments shorter than     495 bp
  45% of sequence in fragments shorter than     561 bp
  50% of sequence in fragments shorter than     634 bp
  55% of sequence in fragments shorter than     710 bp
  60% of sequence in fragments shorter than     796 bp
  65% of sequence in fragments shorter than     892 bp
  70% of sequence in fragments shorter than    1008 bp
  75% of sequence in fragments shorter than    1144 bp
  80% of sequence in fragments shorter than    1310 bp
  85% of sequence in fragments shorter than    1528 bp
  90% of sequence in fragments shorter than    1838 bp
  95% of sequence in fragments shorter than    2380 bp

# HinfI.PvuII (GANTC+CAGCTG) double digest, 9999 thousand fragments (8916054+1084260 sites), 298 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     202 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     328 bp
  35% of sequence in fragments shorter than     364 bp
  40% of sequence in fragments shorter than     408 bp
  45% of sequence in fragments shorter than     454 bp
  50% of sequence in fragments shorter than     504 bp
  55% of sequence in fragments shorter than     557 bp
  60% of sequence in fragments shorter than     616 bp
  65% of sequence in fragments shorter than     679 bp
  70% of sequence in fragments shorter than     747 bp
  75% of sequence in fragments shorter than     828 bp
  80% of sequence in fragments shorter than     928 bp
  85% of sequence in fragments shorter than    1053 bp
  90% of sequence in fragments shorter than    1228 bp
  95% of sequence in fragments shorter than    1522 bp

# BscGI.TspRI (CCCGT+CASTG) double digest, 9995 thousand fragments (1231070+8765240 sites), 298 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     244 bp
  25% of sequence in fragments shorter than     289 bp
  30% of sequence in fragments shorter than     335 bp
  35% of sequence in fragments shorter than     375 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     476 bp
  50% of sequence in fragments shorter than     529 bp
  55% of sequence in fragments shorter than     589 bp
  60% of sequence in fragments shorter than     654 bp
  65% of sequence in fragments shorter than     725 bp
  70% of sequence in fragments shorter than     807 bp
  75% of sequence in fragments shorter than     904 bp
  80% of sequence in fragments shorter than    1015 bp
  85% of sequence in fragments shorter than    1161 bp
  90% of sequence in fragments shorter than    1366 bp
  95% of sequence in fragments shorter than    1708 bp

# BstNI.SnaBI (CCWGG+TACGTA) double digest, 9987 thousand fragments (9855646+132299 sites), 298 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     237 bp
  25% of sequence in fragments shorter than     295 bp
  30% of sequence in fragments shorter than     354 bp
  35% of sequence in fragments shorter than     418 bp
  40% of sequence in fragments shorter than     488 bp
  45% of sequence in fragments shorter than     564 bp
  50% of sequence in fragments shorter than     646 bp
  55% of sequence in fragments shorter than     735 bp
  60% of sequence in fragments shorter than     840 bp
  65% of sequence in fragments shorter than     962 bp
  70% of sequence in fragments shorter than    1104 bp
  75% of sequence in fragments shorter than    1274 bp
  80% of sequence in fragments shorter than    1487 bp
  85% of sequence in fragments shorter than    1778 bp
  90% of sequence in fragments shorter than    2194 bp
  95% of sequence in fragments shorter than    2987 bp

# BstNI.NaeI (CCWGG+GCCGGC) double digest, 9980 thousand fragments (9855646+125692 sites), 298 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     237 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     357 bp
  35% of sequence in fragments shorter than     423 bp
  40% of sequence in fragments shorter than     495 bp
  45% of sequence in fragments shorter than     574 bp
  50% of sequence in fragments shorter than     657 bp
  55% of sequence in fragments shorter than     751 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     987 bp
  70% of sequence in fragments shorter than    1134 bp
  75% of sequence in fragments shorter than    1313 bp
  80% of sequence in fragments shorter than    1542 bp
  85% of sequence in fragments shorter than    1850 bp
  90% of sequence in fragments shorter than    2287 bp
  95% of sequence in fragments shorter than    3147 bp

# BstNI.XhoI (CCWGG+CTCGAG) double digest, 9976 thousand fragments (9855646+121308 sites), 298 bp average fragment length
   5% of sequence in fragments shorter than      84 bp
  10% of sequence in fragments shorter than     131 bp
  15% of sequence in fragments shorter than     180 bp
  20% of sequence in fragments shorter than     238 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     421 bp
  40% of sequence in fragments shorter than     493 bp
  45% of sequence in fragments shorter than     571 bp
  50% of sequence in fragments shorter than     655 bp
  55% of sequence in fragments shorter than     747 bp
  60% of sequence in fragments shorter than     854 bp
  65% of sequence in fragments shorter than     979 bp
  70% of sequence in fragments shorter than    1127 bp
  75% of sequence in fragments shorter than    1304 bp
  80% of sequence in fragments shorter than    1531 bp
  85% of sequence in fragments shorter than    1837 bp
  90% of sequence in fragments shorter than    2270 bp
  95% of sequence in fragments shorter than    3125 bp

# BglII.MboII (AGATCT+GAAGA) double digest, 9973 thousand fragments (774732+9199496 sites), 298 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     302 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     395 bp
  40% of sequence in fragments shorter than     445 bp
  45% of sequence in fragments shorter than     499 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     616 bp
  60% of sequence in fragments shorter than     682 bp
  65% of sequence in fragments shorter than     755 bp
  70% of sequence in fragments shorter than     839 bp
  75% of sequence in fragments shorter than     934 bp
  80% of sequence in fragments shorter than    1046 bp
  85% of sequence in fragments shorter than    1192 bp
  90% of sequence in fragments shorter than    1399 bp
  95% of sequence in fragments shorter than    1751 bp

# MboII.NcoI (GAAGA+CCATGG) double digest, 9958 thousand fragments (9199496+759594 sites), 299 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     251 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     391 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     490 bp
  50% of sequence in fragments shorter than     544 bp
  55% of sequence in fragments shorter than     604 bp
  60% of sequence in fragments shorter than     668 bp
  65% of sequence in fragments shorter than     741 bp
  70% of sequence in fragments shorter than     822 bp
  75% of sequence in fragments shorter than     915 bp
  80% of sequence in fragments shorter than    1025 bp
  85% of sequence in fragments shorter than    1168 bp
  90% of sequence in fragments shorter than    1370 bp
  95% of sequence in fragments shorter than    1715 bp

# AvaI.Hpy188I (CYCGRG+TCNGA) double digest, 9947 thousand fragments (1005714+8942153 sites), 299 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     196 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     284 bp
  30% of sequence in fragments shorter than     328 bp
  35% of sequence in fragments shorter than     367 bp
  40% of sequence in fragments shorter than     414 bp
  45% of sequence in fragments shorter than     462 bp
  50% of sequence in fragments shorter than     514 bp
  55% of sequence in fragments shorter than     569 bp
  60% of sequence in fragments shorter than     628 bp
  65% of sequence in fragments shorter than     696 bp
  70% of sequence in fragments shorter than     772 bp
  75% of sequence in fragments shorter than     859 bp
  80% of sequence in fragments shorter than     963 bp
  85% of sequence in fragments shorter than    1094 bp
  90% of sequence in fragments shorter than    1262 bp
  95% of sequence in fragments shorter than    1561 bp

# AciI.BsrI (CCGC+ACTGG) double digest, 9946 thousand fragments (4206130+5741310 sites), 299 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     158 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     263 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     369 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     480 bp
  45% of sequence in fragments shorter than     540 bp
  50% of sequence in fragments shorter than     605 bp
  55% of sequence in fragments shorter than     674 bp
  60% of sequence in fragments shorter than     746 bp
  65% of sequence in fragments shorter than     832 bp
  70% of sequence in fragments shorter than     927 bp
  75% of sequence in fragments shorter than    1041 bp
  80% of sequence in fragments shorter than    1182 bp
  85% of sequence in fragments shorter than    1362 bp
  90% of sequence in fragments shorter than    1608 bp
  95% of sequence in fragments shorter than    2041 bp

# AvaII.BspKT6I (GGWCC+GATC) double digest, 9946 thousand fragments (2747690+7199434 sites), 299 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     190 bp
  20% of sequence in fragments shorter than     237 bp
  25% of sequence in fragments shorter than     281 bp
  30% of sequence in fragments shorter than     324 bp
  35% of sequence in fragments shorter than     363 bp
  40% of sequence in fragments shorter than     410 bp
  45% of sequence in fragments shorter than     455 bp
  50% of sequence in fragments shorter than     505 bp
  55% of sequence in fragments shorter than     560 bp
  60% of sequence in fragments shorter than     621 bp
  65% of sequence in fragments shorter than     685 bp
  70% of sequence in fragments shorter than     760 bp
  75% of sequence in fragments shorter than     851 bp
  80% of sequence in fragments shorter than     959 bp
  85% of sequence in fragments shorter than    1095 bp
  90% of sequence in fragments shorter than    1292 bp
  95% of sequence in fragments shorter than    1638 bp

# BstNI.ClaI (CCWGG+ATCGAT) double digest, 9941 thousand fragments (9855646+86477 sites), 299 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     181 bp
  20% of sequence in fragments shorter than     238 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     421 bp
  40% of sequence in fragments shorter than     492 bp
  45% of sequence in fragments shorter than     570 bp
  50% of sequence in fragments shorter than     653 bp
  55% of sequence in fragments shorter than     744 bp
  60% of sequence in fragments shorter than     851 bp
  65% of sequence in fragments shorter than     975 bp
  70% of sequence in fragments shorter than    1119 bp
  75% of sequence in fragments shorter than    1294 bp
  80% of sequence in fragments shorter than    1518 bp
  85% of sequence in fragments shorter than    1820 bp
  90% of sequence in fragments shorter than    2246 bp
  95% of sequence in fragments shorter than    3082 bp

# HphI.MlyI (GGTGA+GAGTC) double digest, 9938 thousand fragments (6007336+3931692 sites), 299 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     196 bp
  20% of sequence in fragments shorter than     242 bp
  25% of sequence in fragments shorter than     287 bp
  30% of sequence in fragments shorter than     333 bp
  35% of sequence in fragments shorter than     377 bp
  40% of sequence in fragments shorter than     426 bp
  45% of sequence in fragments shorter than     478 bp
  50% of sequence in fragments shorter than     531 bp
  55% of sequence in fragments shorter than     589 bp
  60% of sequence in fragments shorter than     653 bp
  65% of sequence in fragments shorter than     723 bp
  70% of sequence in fragments shorter than     803 bp
  75% of sequence in fragments shorter than     900 bp
  80% of sequence in fragments shorter than    1021 bp
  85% of sequence in fragments shorter than    1174 bp
  90% of sequence in fragments shorter than    1384 bp
  95% of sequence in fragments shorter than    1773 bp

# BclI.MboII (TGATCA+GAAGA) double digest, 9937 thousand fragments (738785+9199496 sites), 299 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     252 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     390 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     493 bp
  50% of sequence in fragments shorter than     548 bp
  55% of sequence in fragments shorter than     608 bp
  60% of sequence in fragments shorter than     671 bp
  65% of sequence in fragments shorter than     744 bp
  70% of sequence in fragments shorter than     826 bp
  75% of sequence in fragments shorter than     919 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1177 bp
  90% of sequence in fragments shorter than    1379 bp
  95% of sequence in fragments shorter than    1725 bp

# FnuDII.MboII (CGCG+GAAGA) double digest, 9932 thousand fragments (733940+9199496 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     303 bp
  30% of sequence in fragments shorter than     346 bp
  35% of sequence in fragments shorter than     396 bp
  40% of sequence in fragments shorter than     445 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     553 bp
  55% of sequence in fragments shorter than     613 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     751 bp
  70% of sequence in fragments shorter than     833 bp
  75% of sequence in fragments shorter than     925 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1172 bp
  90% of sequence in fragments shorter than    1367 bp
  95% of sequence in fragments shorter than    1696 bp

# BspKT6I.SmlI (GATC+CTYRAG) double digest, 9926 thousand fragments (7199434+2727867 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     194 bp
  20% of sequence in fragments shorter than     243 bp
  25% of sequence in fragments shorter than     289 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     423 bp
  45% of sequence in fragments shorter than     470 bp
  50% of sequence in fragments shorter than     518 bp
  55% of sequence in fragments shorter than     572 bp
  60% of sequence in fragments shorter than     631 bp
  65% of sequence in fragments shorter than     696 bp
  70% of sequence in fragments shorter than     773 bp
  75% of sequence in fragments shorter than     862 bp
  80% of sequence in fragments shorter than     966 bp
  85% of sequence in fragments shorter than    1103 bp
  90% of sequence in fragments shorter than    1297 bp
  95% of sequence in fragments shorter than    1642 bp

# AatII.BstNI (GACGTC+CCWGG) double digest, 9926 thousand fragments (71089+9855646 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     181 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     423 bp
  40% of sequence in fragments shorter than     495 bp
  45% of sequence in fragments shorter than     573 bp
  50% of sequence in fragments shorter than     657 bp
  55% of sequence in fragments shorter than     749 bp
  60% of sequence in fragments shorter than     858 bp
  65% of sequence in fragments shorter than     984 bp
  70% of sequence in fragments shorter than    1130 bp
  75% of sequence in fragments shorter than    1308 bp
  80% of sequence in fragments shorter than    1535 bp
  85% of sequence in fragments shorter than    1842 bp
  90% of sequence in fragments shorter than    2275 bp
  95% of sequence in fragments shorter than    3131 bp

# BstNI.SacII (CCWGG+CCGCGG) double digest, 9922 thousand fragments (9855646+66901 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     181 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     497 bp
  45% of sequence in fragments shorter than     576 bp
  50% of sequence in fragments shorter than     659 bp
  55% of sequence in fragments shorter than     753 bp
  60% of sequence in fragments shorter than     863 bp
  65% of sequence in fragments shorter than     990 bp
  70% of sequence in fragments shorter than    1137 bp
  75% of sequence in fragments shorter than    1317 bp
  80% of sequence in fragments shorter than    1546 bp
  85% of sequence in fragments shorter than    1854 bp
  90% of sequence in fragments shorter than    2292 bp
  95% of sequence in fragments shorter than    3155 bp

# AvaI.HinfI (CYCGRG+GANTC) double digest, 9921 thousand fragments (1005714+8916054 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     252 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     337 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     420 bp
  45% of sequence in fragments shorter than     468 bp
  50% of sequence in fragments shorter than     520 bp
  55% of sequence in fragments shorter than     576 bp
  60% of sequence in fragments shorter than     637 bp
  65% of sequence in fragments shorter than     699 bp
  70% of sequence in fragments shorter than     774 bp
  75% of sequence in fragments shorter than     859 bp
  80% of sequence in fragments shorter than     963 bp
  85% of sequence in fragments shorter than    1093 bp
  90% of sequence in fragments shorter than    1275 bp
  95% of sequence in fragments shorter than    1582 bp

# BspHI.Hpy188I (TCATGA+TCNGA) double digest, 9919 thousand fragments (978290+8942153 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     196 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     286 bp
  30% of sequence in fragments shorter than     331 bp
  35% of sequence in fragments shorter than     372 bp
  40% of sequence in fragments shorter than     420 bp
  45% of sequence in fragments shorter than     468 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     579 bp
  60% of sequence in fragments shorter than     640 bp
  65% of sequence in fragments shorter than     709 bp
  70% of sequence in fragments shorter than     786 bp
  75% of sequence in fragments shorter than     872 bp
  80% of sequence in fragments shorter than     978 bp
  85% of sequence in fragments shorter than    1112 bp
  90% of sequence in fragments shorter than    1297 bp
  95% of sequence in fragments shorter than    1615 bp

# BstNI.PspXI (CCWGG+VCTCGAGB) double digest, 9914 thousand fragments (9855646+59193 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     181 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     424 bp
  40% of sequence in fragments shorter than     496 bp
  45% of sequence in fragments shorter than     575 bp
  50% of sequence in fragments shorter than     658 bp
  55% of sequence in fragments shorter than     751 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     987 bp
  70% of sequence in fragments shorter than    1134 bp
  75% of sequence in fragments shorter than    1312 bp
  80% of sequence in fragments shorter than    1540 bp
  85% of sequence in fragments shorter than    1847 bp
  90% of sequence in fragments shorter than    2283 bp
  95% of sequence in fragments shorter than    3140 bp

# ApoI.SfeI (RAATTY+CTRYAG) double digest, 9912 thousand fragments (6382433+3531009 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     202 bp
  20% of sequence in fragments shorter than     245 bp
  25% of sequence in fragments shorter than     286 bp
  30% of sequence in fragments shorter than     329 bp
  35% of sequence in fragments shorter than     372 bp
  40% of sequence in fragments shorter than     417 bp
  45% of sequence in fragments shorter than     465 bp
  50% of sequence in fragments shorter than     516 bp
  55% of sequence in fragments shorter than     570 bp
  60% of sequence in fragments shorter than     631 bp
  65% of sequence in fragments shorter than     696 bp
  70% of sequence in fragments shorter than     773 bp
  75% of sequence in fragments shorter than     858 bp
  80% of sequence in fragments shorter than     962 bp
  85% of sequence in fragments shorter than    1094 bp
  90% of sequence in fragments shorter than    1284 bp
  95% of sequence in fragments shorter than    1600 bp

# BsaAI.MboII (YACGTR+GAAGA) double digest, 9912 thousand fragments (713801+9199496 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     253 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     393 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     495 bp
  50% of sequence in fragments shorter than     549 bp
  55% of sequence in fragments shorter than     608 bp
  60% of sequence in fragments shorter than     673 bp
  65% of sequence in fragments shorter than     745 bp
  70% of sequence in fragments shorter than     826 bp
  75% of sequence in fragments shorter than     919 bp
  80% of sequence in fragments shorter than    1027 bp
  85% of sequence in fragments shorter than    1169 bp
  90% of sequence in fragments shorter than    1369 bp
  95% of sequence in fragments shorter than    1708 bp

# AgeI.BstNI (ACCGGT+CCWGG) double digest, 9908 thousand fragments (53386+9855646 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     132 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     424 bp
  40% of sequence in fragments shorter than     496 bp
  45% of sequence in fragments shorter than     574 bp
  50% of sequence in fragments shorter than     657 bp
  55% of sequence in fragments shorter than     750 bp
  60% of sequence in fragments shorter than     859 bp
  65% of sequence in fragments shorter than     985 bp
  70% of sequence in fragments shorter than    1132 bp
  75% of sequence in fragments shorter than    1310 bp
  80% of sequence in fragments shorter than    1537 bp
  85% of sequence in fragments shorter than    1844 bp
  90% of sequence in fragments shorter than    2279 bp
  95% of sequence in fragments shorter than    3135 bp

# BceAI.MboII (ACGGC+GAAGA) double digest, 9904 thousand fragments (705178+9199496 sites), 300 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     253 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     393 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     496 bp
  50% of sequence in fragments shorter than     551 bp
  55% of sequence in fragments shorter than     611 bp
  60% of sequence in fragments shorter than     677 bp
  65% of sequence in fragments shorter than     749 bp
  70% of sequence in fragments shorter than     831 bp
  75% of sequence in fragments shorter than     924 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1174 bp
  90% of sequence in fragments shorter than    1372 bp
  95% of sequence in fragments shorter than    1709 bp

# BstNI.PmeI (CCWGG+GTTTAAAC) double digest, 9897 thousand fragments (9855646+42094 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     239 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     424 bp
  40% of sequence in fragments shorter than     495 bp
  45% of sequence in fragments shorter than     574 bp
  50% of sequence in fragments shorter than     657 bp
  55% of sequence in fragments shorter than     749 bp
  60% of sequence in fragments shorter than     857 bp
  65% of sequence in fragments shorter than     982 bp
  70% of sequence in fragments shorter than    1128 bp
  75% of sequence in fragments shorter than    1305 bp
  80% of sequence in fragments shorter than    1530 bp
  85% of sequence in fragments shorter than    1836 bp
  90% of sequence in fragments shorter than    2267 bp
  95% of sequence in fragments shorter than    3115 bp

# BciVI.MboII (GTATCC+GAAGA) double digest, 9895 thousand fragments (696093+9199496 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     252 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     394 bp
  40% of sequence in fragments shorter than     444 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     553 bp
  55% of sequence in fragments shorter than     613 bp
  60% of sequence in fragments shorter than     678 bp
  65% of sequence in fragments shorter than     751 bp
  70% of sequence in fragments shorter than     834 bp
  75% of sequence in fragments shorter than     926 bp
  80% of sequence in fragments shorter than    1041 bp
  85% of sequence in fragments shorter than    1187 bp
  90% of sequence in fragments shorter than    1393 bp
  95% of sequence in fragments shorter than    1745 bp

# BspHI.HinfI (TCATGA+GANTC) double digest, 9893 thousand fragments (978290+8916054 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     292 bp
  30% of sequence in fragments shorter than     332 bp
  35% of sequence in fragments shorter than     368 bp
  40% of sequence in fragments shorter than     413 bp
  45% of sequence in fragments shorter than     459 bp
  50% of sequence in fragments shorter than     510 bp
  55% of sequence in fragments shorter than     563 bp
  60% of sequence in fragments shorter than     619 bp
  65% of sequence in fragments shorter than     683 bp
  70% of sequence in fragments shorter than     753 bp
  75% of sequence in fragments shorter than     835 bp
  80% of sequence in fragments shorter than     935 bp
  85% of sequence in fragments shorter than    1060 bp
  90% of sequence in fragments shorter than    1230 bp
  95% of sequence in fragments shorter than    1518 bp

# BstNI.SalI (CCWGG+GTCGAC) double digest, 9886 thousand fragments (9855646+31396 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     497 bp
  45% of sequence in fragments shorter than     576 bp
  50% of sequence in fragments shorter than     659 bp
  55% of sequence in fragments shorter than     752 bp
  60% of sequence in fragments shorter than     861 bp
  65% of sequence in fragments shorter than     987 bp
  70% of sequence in fragments shorter than    1134 bp
  75% of sequence in fragments shorter than    1313 bp
  80% of sequence in fragments shorter than    1541 bp
  85% of sequence in fragments shorter than    1849 bp
  90% of sequence in fragments shorter than    2284 bp
  95% of sequence in fragments shorter than    3141 bp

# HaeIII.PstI (GGCC+CTGCAG) double digest, 9883 thousand fragments (8562230+1321469 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     151 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     434 bp
  40% of sequence in fragments shorter than     500 bp
  45% of sequence in fragments shorter than     568 bp
  50% of sequence in fragments shorter than     643 bp
  55% of sequence in fragments shorter than     724 bp
  60% of sequence in fragments shorter than     818 bp
  65% of sequence in fragments shorter than     919 bp
  70% of sequence in fragments shorter than    1039 bp
  75% of sequence in fragments shorter than    1184 bp
  80% of sequence in fragments shorter than    1352 bp
  85% of sequence in fragments shorter than    1577 bp
  90% of sequence in fragments shorter than    1896 bp
  95% of sequence in fragments shorter than    2453 bp

# BstNI.MluI (CCWGG+ACGCGT) double digest, 9876 thousand fragments (9855646+21415 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     498 bp
  45% of sequence in fragments shorter than     577 bp
  50% of sequence in fragments shorter than     659 bp
  55% of sequence in fragments shorter than     754 bp
  60% of sequence in fragments shorter than     863 bp
  65% of sequence in fragments shorter than     990 bp
  70% of sequence in fragments shorter than    1137 bp
  75% of sequence in fragments shorter than    1316 bp
  80% of sequence in fragments shorter than    1545 bp
  85% of sequence in fragments shorter than    1853 bp
  90% of sequence in fragments shorter than    2290 bp
  95% of sequence in fragments shorter than    3150 bp

# AvaIII.Hpy188I (ATGCAT+TCNGA) double digest, 9875 thousand fragments (933357+8942153 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     197 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     288 bp
  30% of sequence in fragments shorter than     334 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     474 bp
  50% of sequence in fragments shorter than     528 bp
  55% of sequence in fragments shorter than     584 bp
  60% of sequence in fragments shorter than     647 bp
  65% of sequence in fragments shorter than     717 bp
  70% of sequence in fragments shorter than     796 bp
  75% of sequence in fragments shorter than     886 bp
  80% of sequence in fragments shorter than     995 bp
  85% of sequence in fragments shorter than    1132 bp
  90% of sequence in fragments shorter than    1308 bp
  95% of sequence in fragments shorter than    1629 bp

# BstNI.SgrAI (CCWGG+CRCCGGYG) double digest, 9870 thousand fragments (9855646+15270 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     498 bp
  45% of sequence in fragments shorter than     577 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     754 bp
  60% of sequence in fragments shorter than     864 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1138 bp
  75% of sequence in fragments shorter than    1318 bp
  80% of sequence in fragments shorter than    1547 bp
  85% of sequence in fragments shorter than    1855 bp
  90% of sequence in fragments shorter than    2294 bp
  95% of sequence in fragments shorter than    3156 bp

# BstNI.NruI (CCWGG+TCGCGA) double digest, 9870 thousand fragments (9855646+14872 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     498 bp
  45% of sequence in fragments shorter than     577 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     754 bp
  60% of sequence in fragments shorter than     864 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1138 bp
  75% of sequence in fragments shorter than    1318 bp
  80% of sequence in fragments shorter than    1547 bp
  85% of sequence in fragments shorter than    1854 bp
  90% of sequence in fragments shorter than    2292 bp
  95% of sequence in fragments shorter than    3155 bp

# BstNI.PvuI (CCWGG+CGATCG) double digest, 9869 thousand fragments (9855646+14186 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     498 bp
  45% of sequence in fragments shorter than     577 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     754 bp
  60% of sequence in fragments shorter than     864 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1138 bp
  75% of sequence in fragments shorter than    1317 bp
  80% of sequence in fragments shorter than    1546 bp
  85% of sequence in fragments shorter than    1852 bp
  90% of sequence in fragments shorter than    2283 bp
  95% of sequence in fragments shorter than    3129 bp

# BstNI.FseI (CCWGG+GGCCGGCC) double digest, 9868 thousand fragments (9855646+13662 sites), 301 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     499 bp
  45% of sequence in fragments shorter than     578 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     755 bp
  60% of sequence in fragments shorter than     865 bp
  65% of sequence in fragments shorter than     992 bp
  70% of sequence in fragments shorter than    1139 bp
  75% of sequence in fragments shorter than    1319 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1856 bp
  90% of sequence in fragments shorter than    2295 bp
  95% of sequence in fragments shorter than    3157 bp

# Hpy188I.StuI (TCNGA+AGGCCT) double digest, 9867 thousand fragments (8942153+925729 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     154 bp
  15% of sequence in fragments shorter than     197 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     290 bp
  30% of sequence in fragments shorter than     336 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     430 bp
  45% of sequence in fragments shorter than     481 bp
  50% of sequence in fragments shorter than     537 bp
  55% of sequence in fragments shorter than     596 bp
  60% of sequence in fragments shorter than     663 bp
  65% of sequence in fragments shorter than     736 bp
  70% of sequence in fragments shorter than     817 bp
  75% of sequence in fragments shorter than     910 bp
  80% of sequence in fragments shorter than    1018 bp
  85% of sequence in fragments shorter than    1160 bp
  90% of sequence in fragments shorter than    1352 bp
  95% of sequence in fragments shorter than    1686 bp

# AccI.TspRI (GTMKAC+CASTG) double digest, 9865 thousand fragments (1101063+8765240 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     336 bp
  35% of sequence in fragments shorter than     380 bp
  40% of sequence in fragments shorter than     426 bp
  45% of sequence in fragments shorter than     475 bp
  50% of sequence in fragments shorter than     525 bp
  55% of sequence in fragments shorter than     580 bp
  60% of sequence in fragments shorter than     642 bp
  65% of sequence in fragments shorter than     708 bp
  70% of sequence in fragments shorter than     786 bp
  75% of sequence in fragments shorter than     877 bp
  80% of sequence in fragments shorter than     982 bp
  85% of sequence in fragments shorter than    1121 bp
  90% of sequence in fragments shorter than    1316 bp
  95% of sequence in fragments shorter than    1641 bp

# BstNI.NotI (CCWGG+GCGGCCGC) double digest, 9864 thousand fragments (9855646+9795 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     499 bp
  45% of sequence in fragments shorter than     578 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     755 bp
  60% of sequence in fragments shorter than     865 bp
  65% of sequence in fragments shorter than     992 bp
  70% of sequence in fragments shorter than    1139 bp
  75% of sequence in fragments shorter than    1319 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1856 bp
  90% of sequence in fragments shorter than    2295 bp
  95% of sequence in fragments shorter than    3157 bp

# BstNI.RsrII (CCWGG+CGGWCCG) double digest, 9864 thousand fragments (9855646+9764 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     182 bp
  20% of sequence in fragments shorter than     240 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     499 bp
  45% of sequence in fragments shorter than     578 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     755 bp
  60% of sequence in fragments shorter than     864 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1139 bp
  75% of sequence in fragments shorter than    1319 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1856 bp
  90% of sequence in fragments shorter than    2294 bp
  95% of sequence in fragments shorter than    3157 bp

# BscAI.BthCI (GCATC+GCNGC) double digest, 9862 thousand fragments (3652926+6209929 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     330 bp
  30% of sequence in fragments shorter than     385 bp
  35% of sequence in fragments shorter than     445 bp
  40% of sequence in fragments shorter than     509 bp
  45% of sequence in fragments shorter than     574 bp
  50% of sequence in fragments shorter than     642 bp
  55% of sequence in fragments shorter than     714 bp
  60% of sequence in fragments shorter than     797 bp
  65% of sequence in fragments shorter than     884 bp
  70% of sequence in fragments shorter than     990 bp
  75% of sequence in fragments shorter than    1110 bp
  80% of sequence in fragments shorter than    1252 bp
  85% of sequence in fragments shorter than    1438 bp
  90% of sequence in fragments shorter than    1690 bp
  95% of sequence in fragments shorter than    2136 bp

# AscI.BstNI (GGCGCGCC+CCWGG) double digest, 9859 thousand fragments (4751+9855646 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than      85 bp
  10% of sequence in fragments shorter than     133 bp
  15% of sequence in fragments shorter than     183 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     361 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     499 bp
  45% of sequence in fragments shorter than     578 bp
  50% of sequence in fragments shorter than     661 bp
  55% of sequence in fragments shorter than     755 bp
  60% of sequence in fragments shorter than     865 bp
  65% of sequence in fragments shorter than     992 bp
  70% of sequence in fragments shorter than    1139 bp
  75% of sequence in fragments shorter than    1319 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1856 bp
  90% of sequence in fragments shorter than    2295 bp
  95% of sequence in fragments shorter than    3157 bp

# PpuMI.TspRI (RGGWCCY+CASTG) double digest, 9849 thousand fragments (1085138+8765240 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     294 bp
  30% of sequence in fragments shorter than     339 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     484 bp
  50% of sequence in fragments shorter than     539 bp
  55% of sequence in fragments shorter than     599 bp
  60% of sequence in fragments shorter than     664 bp
  65% of sequence in fragments shorter than     736 bp
  70% of sequence in fragments shorter than     820 bp
  75% of sequence in fragments shorter than     920 bp
  80% of sequence in fragments shorter than    1033 bp
  85% of sequence in fragments shorter than    1181 bp
  90% of sequence in fragments shorter than    1398 bp
  95% of sequence in fragments shorter than    1782 bp

# PvuII.TspRI (CAGCTG+CASTG) double digest, 9849 thousand fragments (1084260+8765240 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     294 bp
  30% of sequence in fragments shorter than     339 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     484 bp
  50% of sequence in fragments shorter than     539 bp
  55% of sequence in fragments shorter than     598 bp
  60% of sequence in fragments shorter than     663 bp
  65% of sequence in fragments shorter than     736 bp
  70% of sequence in fragments shorter than     819 bp
  75% of sequence in fragments shorter than     918 bp
  80% of sequence in fragments shorter than    1030 bp
  85% of sequence in fragments shorter than    1178 bp
  90% of sequence in fragments shorter than    1396 bp
  95% of sequence in fragments shorter than    1785 bp

# AvaIII.HinfI (ATGCAT+GANTC) double digest, 9848 thousand fragments (933357+8916054 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     292 bp
  30% of sequence in fragments shorter than     331 bp
  35% of sequence in fragments shorter than     367 bp
  40% of sequence in fragments shorter than     410 bp
  45% of sequence in fragments shorter than     455 bp
  50% of sequence in fragments shorter than     505 bp
  55% of sequence in fragments shorter than     556 bp
  60% of sequence in fragments shorter than     614 bp
  65% of sequence in fragments shorter than     675 bp
  70% of sequence in fragments shorter than     742 bp
  75% of sequence in fragments shorter than     822 bp
  80% of sequence in fragments shorter than     919 bp
  85% of sequence in fragments shorter than    1039 bp
  90% of sequence in fragments shorter than    1206 bp
  95% of sequence in fragments shorter than    1484 bp

# Hpy188I.NdeI (TCNGA+CATATG) double digest, 9845 thousand fragments (8942153+903360 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     197 bp
  20% of sequence in fragments shorter than     241 bp
  25% of sequence in fragments shorter than     286 bp
  30% of sequence in fragments shorter than     331 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     423 bp
  45% of sequence in fragments shorter than     472 bp
  50% of sequence in fragments shorter than     525 bp
  55% of sequence in fragments shorter than     582 bp
  60% of sequence in fragments shorter than     643 bp
  65% of sequence in fragments shorter than     709 bp
  70% of sequence in fragments shorter than     787 bp
  75% of sequence in fragments shorter than     875 bp
  80% of sequence in fragments shorter than     983 bp
  85% of sequence in fragments shorter than    1112 bp
  90% of sequence in fragments shorter than    1298 bp
  95% of sequence in fragments shorter than    1618 bp

# AflII.MboII (CTTAAG+GAAGA) double digest, 9842 thousand fragments (643284+9199496 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     303 bp
  30% of sequence in fragments shorter than     346 bp
  35% of sequence in fragments shorter than     397 bp
  40% of sequence in fragments shorter than     447 bp
  45% of sequence in fragments shorter than     501 bp
  50% of sequence in fragments shorter than     556 bp
  55% of sequence in fragments shorter than     617 bp
  60% of sequence in fragments shorter than     683 bp
  65% of sequence in fragments shorter than     756 bp
  70% of sequence in fragments shorter than     838 bp
  75% of sequence in fragments shorter than     931 bp
  80% of sequence in fragments shorter than    1046 bp
  85% of sequence in fragments shorter than    1193 bp
  90% of sequence in fragments shorter than    1399 bp
  95% of sequence in fragments shorter than    1752 bp

# HinfI.StuI (GANTC+AGGCCT) double digest, 9841 thousand fragments (8916054+925729 sites), 302 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     156 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     289 bp
  30% of sequence in fragments shorter than     329 bp
  35% of sequence in fragments shorter than     371 bp
  40% of sequence in fragments shorter than     416 bp
  45% of sequence in fragments shorter than     464 bp
  50% of sequence in fragments shorter than     514 bp
  55% of sequence in fragments shorter than     569 bp
  60% of sequence in fragments shorter than     628 bp
  65% of sequence in fragments shorter than     693 bp
  70% of sequence in fragments shorter than     765 bp
  75% of sequence in fragments shorter than     851 bp
  80% of sequence in fragments shorter than     954 bp
  85% of sequence in fragments shorter than    1082 bp
  90% of sequence in fragments shorter than    1260 bp
  95% of sequence in fragments shorter than    1563 bp

# HaeII.MboII (RGCGCY+GAAGA) double digest, 9829 thousand fragments (630101+9199496 sites), 303 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     303 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     396 bp
  40% of sequence in fragments shorter than     446 bp
  45% of sequence in fragments shorter than     500 bp
  50% of sequence in fragments shorter than     555 bp
  55% of sequence in fragments shorter than     615 bp
  60% of sequence in fragments shorter than     681 bp
  65% of sequence in fragments shorter than     753 bp
  70% of sequence in fragments shorter than     835 bp
  75% of sequence in fragments shorter than     928 bp
  80% of sequence in fragments shorter than    1035 bp
  85% of sequence in fragments shorter than    1176 bp
  90% of sequence in fragments shorter than    1372 bp
  95% of sequence in fragments shorter than    1704 bp

# BccI.BscAI (CCATC+GCATC) double digest, 9823 thousand fragments (6170927+3652926 sites), 303 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     339 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     484 bp
  50% of sequence in fragments shorter than     538 bp
  55% of sequence in fragments shorter than     597 bp
  60% of sequence in fragments shorter than     661 bp
  65% of sequence in fragments shorter than     732 bp
  70% of sequence in fragments shorter than     812 bp
  75% of sequence in fragments shorter than     907 bp
  80% of sequence in fragments shorter than    1022 bp
  85% of sequence in fragments shorter than    1167 bp
  90% of sequence in fragments shorter than    1379 bp
  95% of sequence in fragments shorter than    1750 bp

# HinfI.NdeI (GANTC+CATATG) double digest, 9818 thousand fragments (8916054+903360 sites), 303 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     333 bp
  35% of sequence in fragments shorter than     367 bp
  40% of sequence in fragments shorter than     412 bp
  45% of sequence in fragments shorter than     458 bp
  50% of sequence in fragments shorter than     507 bp
  55% of sequence in fragments shorter than     557 bp
  60% of sequence in fragments shorter than     614 bp
  65% of sequence in fragments shorter than     676 bp
  70% of sequence in fragments shorter than     743 bp
  75% of sequence in fragments shorter than     824 bp
  80% of sequence in fragments shorter than     920 bp
  85% of sequence in fragments shorter than    1041 bp
  90% of sequence in fragments shorter than    1208 bp
  95% of sequence in fragments shorter than    1488 bp

# AvaII.Hin4II (GGWCC+CCTTC) double digest, 9807 thousand fragments (2747690+7059915 sites), 303 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     366 bp
  35% of sequence in fragments shorter than     420 bp
  40% of sequence in fragments shorter than     480 bp
  45% of sequence in fragments shorter than     537 bp
  50% of sequence in fragments shorter than     604 bp
  55% of sequence in fragments shorter than     676 bp
  60% of sequence in fragments shorter than     754 bp
  65% of sequence in fragments shorter than     843 bp
  70% of sequence in fragments shorter than     944 bp
  75% of sequence in fragments shorter than    1056 bp
  80% of sequence in fragments shorter than    1201 bp
  85% of sequence in fragments shorter than    1382 bp
  90% of sequence in fragments shorter than    1636 bp
  95% of sequence in fragments shorter than    2065 bp

# MboII.SacI (GAAGA+GAGCTC) double digest, 9802 thousand fragments (9199496+603323 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     304 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     449 bp
  45% of sequence in fragments shorter than     503 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     620 bp
  60% of sequence in fragments shorter than     687 bp
  65% of sequence in fragments shorter than     760 bp
  70% of sequence in fragments shorter than     843 bp
  75% of sequence in fragments shorter than     939 bp
  80% of sequence in fragments shorter than    1050 bp
  85% of sequence in fragments shorter than    1195 bp
  90% of sequence in fragments shorter than    1398 bp
  95% of sequence in fragments shorter than    1744 bp

# HindIII.Hpy188I (AAGCTT+TCNGA) double digest, 9802 thousand fragments (860365+8942153 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     243 bp
  25% of sequence in fragments shorter than     290 bp
  30% of sequence in fragments shorter than     337 bp
  35% of sequence in fragments shorter than     380 bp
  40% of sequence in fragments shorter than     429 bp
  45% of sequence in fragments shorter than     479 bp
  50% of sequence in fragments shorter than     534 bp
  55% of sequence in fragments shorter than     593 bp
  60% of sequence in fragments shorter than     658 bp
  65% of sequence in fragments shorter than     730 bp
  70% of sequence in fragments shorter than     810 bp
  75% of sequence in fragments shorter than     903 bp
  80% of sequence in fragments shorter than    1014 bp
  85% of sequence in fragments shorter than    1147 bp
  90% of sequence in fragments shorter than    1339 bp
  95% of sequence in fragments shorter than    1670 bp

# AvrII.MboII (CCTAGG+GAAGA) double digest, 9793 thousand fragments (594956+9199496 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     348 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     449 bp
  45% of sequence in fragments shorter than     503 bp
  50% of sequence in fragments shorter than     558 bp
  55% of sequence in fragments shorter than     619 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     759 bp
  70% of sequence in fragments shorter than     841 bp
  75% of sequence in fragments shorter than     937 bp
  80% of sequence in fragments shorter than    1046 bp
  85% of sequence in fragments shorter than    1191 bp
  90% of sequence in fragments shorter than    1394 bp
  95% of sequence in fragments shorter than    1737 bp

# BscGI.HaeIII (CCCGT+GGCC) double digest, 9792 thousand fragments (1231070+8562230 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than      89 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     387 bp
  35% of sequence in fragments shorter than     450 bp
  40% of sequence in fragments shorter than     519 bp
  45% of sequence in fragments shorter than     592 bp
  50% of sequence in fragments shorter than     674 bp
  55% of sequence in fragments shorter than     762 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     968 bp
  70% of sequence in fragments shorter than    1096 bp
  75% of sequence in fragments shorter than    1248 bp
  80% of sequence in fragments shorter than    1427 bp
  85% of sequence in fragments shorter than    1667 bp
  90% of sequence in fragments shorter than    2005 bp
  95% of sequence in fragments shorter than    2601 bp

# Hpy188I.XbaI (TCNGA+TCTAGA) double digest, 9792 thousand fragments (8942153+850998 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     244 bp
  25% of sequence in fragments shorter than     291 bp
  30% of sequence in fragments shorter than     338 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     430 bp
  45% of sequence in fragments shorter than     481 bp
  50% of sequence in fragments shorter than     536 bp
  55% of sequence in fragments shorter than     593 bp
  60% of sequence in fragments shorter than     654 bp
  65% of sequence in fragments shorter than     723 bp
  70% of sequence in fragments shorter than     803 bp
  75% of sequence in fragments shorter than     894 bp
  80% of sequence in fragments shorter than    1004 bp
  85% of sequence in fragments shorter than    1142 bp
  90% of sequence in fragments shorter than    1331 bp
  95% of sequence in fragments shorter than    1658 bp

# EcoRI.Hpy188I (GAATTC+TCNGA) double digest, 9789 thousand fragments (847401+8942153 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     198 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     435 bp
  45% of sequence in fragments shorter than     487 bp
  50% of sequence in fragments shorter than     543 bp
  55% of sequence in fragments shorter than     600 bp
  60% of sequence in fragments shorter than     666 bp
  65% of sequence in fragments shorter than     739 bp
  70% of sequence in fragments shorter than     820 bp
  75% of sequence in fragments shorter than     914 bp
  80% of sequence in fragments shorter than    1026 bp
  85% of sequence in fragments shorter than    1167 bp
  90% of sequence in fragments shorter than    1351 bp
  95% of sequence in fragments shorter than    1683 bp

# Hin4II.SmlI (CCTTC+CTYRAG) double digest, 9787 thousand fragments (7059915+2727867 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     397 bp
  40% of sequence in fragments shorter than     446 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     552 bp
  55% of sequence in fragments shorter than     613 bp
  60% of sequence in fragments shorter than     678 bp
  65% of sequence in fragments shorter than     750 bp
  70% of sequence in fragments shorter than     835 bp
  75% of sequence in fragments shorter than     931 bp
  80% of sequence in fragments shorter than    1042 bp
  85% of sequence in fragments shorter than    1191 bp
  90% of sequence in fragments shorter than    1396 bp
  95% of sequence in fragments shorter than    1752 bp

# HindIII.HinfI (AAGCTT+GANTC) double digest, 9775 thousand fragments (860365+8916054 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     251 bp
  25% of sequence in fragments shorter than     294 bp
  30% of sequence in fragments shorter than     333 bp
  35% of sequence in fragments shorter than     369 bp
  40% of sequence in fragments shorter than     413 bp
  45% of sequence in fragments shorter than     460 bp
  50% of sequence in fragments shorter than     511 bp
  55% of sequence in fragments shorter than     564 bp
  60% of sequence in fragments shorter than     622 bp
  65% of sequence in fragments shorter than     683 bp
  70% of sequence in fragments shorter than     752 bp
  75% of sequence in fragments shorter than     835 bp
  80% of sequence in fragments shorter than     934 bp
  85% of sequence in fragments shorter than    1058 bp
  90% of sequence in fragments shorter than    1229 bp
  95% of sequence in fragments shorter than    1517 bp

# HgaI.MboII (GACGC+GAAGA) double digest, 9770 thousand fragments (571889+9199496 sites), 304 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     400 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     560 bp
  55% of sequence in fragments shorter than     622 bp
  60% of sequence in fragments shorter than     688 bp
  65% of sequence in fragments shorter than     762 bp
  70% of sequence in fragments shorter than     845 bp
  75% of sequence in fragments shorter than     941 bp
  80% of sequence in fragments shorter than    1052 bp
  85% of sequence in fragments shorter than    1197 bp
  90% of sequence in fragments shorter than    1399 bp
  95% of sequence in fragments shorter than    1741 bp

# AvaI.TspRI (CYCGRG+CASTG) double digest, 9770 thousand fragments (1005714+8765240 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     304 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     562 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     692 bp
  65% of sequence in fragments shorter than     769 bp
  70% of sequence in fragments shorter than     857 bp
  75% of sequence in fragments shorter than     955 bp
  80% of sequence in fragments shorter than    1078 bp
  85% of sequence in fragments shorter than    1238 bp
  90% of sequence in fragments shorter than    1467 bp
  95% of sequence in fragments shorter than    1877 bp

# EciI.MboII (GGCGGA+GAAGA) double digest, 9770 thousand fragments (571273+9199496 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     302 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     440 bp
  45% of sequence in fragments shorter than     492 bp
  50% of sequence in fragments shorter than     545 bp
  55% of sequence in fragments shorter than     604 bp
  60% of sequence in fragments shorter than     667 bp
  65% of sequence in fragments shorter than     738 bp
  70% of sequence in fragments shorter than     817 bp
  75% of sequence in fragments shorter than     906 bp
  80% of sequence in fragments shorter than    1014 bp
  85% of sequence in fragments shorter than    1145 bp
  90% of sequence in fragments shorter than    1334 bp
  95% of sequence in fragments shorter than    1652 bp

# HinfI.XbaI (GANTC+TCTAGA) double digest, 9766 thousand fragments (8916054+850998 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     337 bp
  35% of sequence in fragments shorter than     375 bp
  40% of sequence in fragments shorter than     421 bp
  45% of sequence in fragments shorter than     468 bp
  50% of sequence in fragments shorter than     519 bp
  55% of sequence in fragments shorter than     572 bp
  60% of sequence in fragments shorter than     631 bp
  65% of sequence in fragments shorter than     694 bp
  70% of sequence in fragments shorter than     766 bp
  75% of sequence in fragments shorter than     849 bp
  80% of sequence in fragments shorter than     949 bp
  85% of sequence in fragments shorter than    1076 bp
  90% of sequence in fragments shorter than    1250 bp
  95% of sequence in fragments shorter than    1543 bp

# MboII.MfeI (GAAGA+CAATTG) double digest, 9763 thousand fragments (9199496+564303 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     401 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     506 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     691 bp
  65% of sequence in fragments shorter than     766 bp
  70% of sequence in fragments shorter than     849 bp
  75% of sequence in fragments shorter than     947 bp
  80% of sequence in fragments shorter than    1058 bp
  85% of sequence in fragments shorter than    1207 bp
  90% of sequence in fragments shorter than    1416 bp
  95% of sequence in fragments shorter than    1774 bp

# EcoRI.HinfI (GAATTC+GANTC) double digest, 9762 thousand fragments (847401+8916054 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     294 bp
  30% of sequence in fragments shorter than     333 bp
  35% of sequence in fragments shorter than     373 bp
  40% of sequence in fragments shorter than     417 bp
  45% of sequence in fragments shorter than     464 bp
  50% of sequence in fragments shorter than     515 bp
  55% of sequence in fragments shorter than     568 bp
  60% of sequence in fragments shorter than     627 bp
  65% of sequence in fragments shorter than     690 bp
  70% of sequence in fragments shorter than     761 bp
  75% of sequence in fragments shorter than     843 bp
  80% of sequence in fragments shorter than     943 bp
  85% of sequence in fragments shorter than    1069 bp
  90% of sequence in fragments shorter than    1240 bp
  95% of sequence in fragments shorter than    1530 bp

# BsrI.Tsp45I (ACTGG+GTSAC) double digest, 9762 thousand fragments (5741310+4021761 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     302 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     390 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     487 bp
  50% of sequence in fragments shorter than     541 bp
  55% of sequence in fragments shorter than     599 bp
  60% of sequence in fragments shorter than     662 bp
  65% of sequence in fragments shorter than     724 bp
  70% of sequence in fragments shorter than     802 bp
  75% of sequence in fragments shorter than     889 bp
  80% of sequence in fragments shorter than    1000 bp
  85% of sequence in fragments shorter than    1137 bp
  90% of sequence in fragments shorter than    1336 bp
  95% of sequence in fragments shorter than    1678 bp

# MboII.SphI (GAAGA+GCATGC) double digest, 9749 thousand fragments (9199496+550952 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     397 bp
  40% of sequence in fragments shorter than     447 bp
  45% of sequence in fragments shorter than     501 bp
  50% of sequence in fragments shorter than     555 bp
  55% of sequence in fragments shorter than     615 bp
  60% of sequence in fragments shorter than     681 bp
  65% of sequence in fragments shorter than     754 bp
  70% of sequence in fragments shorter than     836 bp
  75% of sequence in fragments shorter than     930 bp
  80% of sequence in fragments shorter than    1038 bp
  85% of sequence in fragments shorter than    1181 bp
  90% of sequence in fragments shorter than    1380 bp
  95% of sequence in fragments shorter than    1718 bp

# BsmAI.StyI (GTCTC+CCWWGG) double digest, 9745 thousand fragments (6631585+3114107 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     300 bp
  30% of sequence in fragments shorter than     352 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     459 bp
  45% of sequence in fragments shorter than     515 bp
  50% of sequence in fragments shorter than     575 bp
  55% of sequence in fragments shorter than     639 bp
  60% of sequence in fragments shorter than     713 bp
  65% of sequence in fragments shorter than     789 bp
  70% of sequence in fragments shorter than     885 bp
  75% of sequence in fragments shorter than     997 bp
  80% of sequence in fragments shorter than    1132 bp
  85% of sequence in fragments shorter than    1312 bp
  90% of sequence in fragments shorter than    1567 bp
  95% of sequence in fragments shorter than    2042 bp

# BspHI.TspRI (TCATGA+CASTG) double digest, 9743 thousand fragments (978290+8765240 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     295 bp
  30% of sequence in fragments shorter than     339 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     431 bp
  45% of sequence in fragments shorter than     481 bp
  50% of sequence in fragments shorter than     534 bp
  55% of sequence in fragments shorter than     591 bp
  60% of sequence in fragments shorter than     653 bp
  65% of sequence in fragments shorter than     722 bp
  70% of sequence in fragments shorter than     802 bp
  75% of sequence in fragments shorter than     895 bp
  80% of sequence in fragments shorter than    1006 bp
  85% of sequence in fragments shorter than    1147 bp
  90% of sequence in fragments shorter than    1354 bp
  95% of sequence in fragments shorter than    1706 bp

# MboII.ScaI (GAAGA+AGTACT) double digest, 9742 thousand fragments (9199496+543793 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     401 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     561 bp
  55% of sequence in fragments shorter than     622 bp
  60% of sequence in fragments shorter than     689 bp
  65% of sequence in fragments shorter than     763 bp
  70% of sequence in fragments shorter than     846 bp
  75% of sequence in fragments shorter than     943 bp
  80% of sequence in fragments shorter than    1054 bp
  85% of sequence in fragments shorter than    1202 bp
  90% of sequence in fragments shorter than    1409 bp
  95% of sequence in fragments shorter than    1763 bp

# BthCI.SfeI (GCNGC+CTRYAG) double digest, 9740 thousand fragments (6209929+3531009 sites), 305 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     240 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     351 bp
  30% of sequence in fragments shorter than     409 bp
  35% of sequence in fragments shorter than     468 bp
  40% of sequence in fragments shorter than     530 bp
  45% of sequence in fragments shorter than     596 bp
  50% of sequence in fragments shorter than     665 bp
  55% of sequence in fragments shorter than     740 bp
  60% of sequence in fragments shorter than     825 bp
  65% of sequence in fragments shorter than     915 bp
  70% of sequence in fragments shorter than    1010 bp
  75% of sequence in fragments shorter than    1127 bp
  80% of sequence in fragments shorter than    1269 bp
  85% of sequence in fragments shorter than    1452 bp
  90% of sequence in fragments shorter than    1712 bp
  95% of sequence in fragments shorter than    2157 bp

# BsmAI.NspI (GTCTC+RCATGY) double digest, 9735 thousand fragments (6631585+3104359 sites), 306 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     300 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     619 bp
  60% of sequence in fragments shorter than     686 bp
  65% of sequence in fragments shorter than     761 bp
  70% of sequence in fragments shorter than     848 bp
  75% of sequence in fragments shorter than     948 bp
  80% of sequence in fragments shorter than    1070 bp
  85% of sequence in fragments shorter than    1227 bp
  90% of sequence in fragments shorter than    1438 bp
  95% of sequence in fragments shorter than    1837 bp

# BglII.Hpy188I (AGATCT+TCNGA) double digest, 9716 thousand fragments (774732+8942153 sites), 306 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     388 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     490 bp
  50% of sequence in fragments shorter than     545 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     671 bp
  65% of sequence in fragments shorter than     744 bp
  70% of sequence in fragments shorter than     824 bp
  75% of sequence in fragments shorter than     918 bp
  80% of sequence in fragments shorter than    1031 bp
  85% of sequence in fragments shorter than    1174 bp
  90% of sequence in fragments shorter than    1358 bp
  95% of sequence in fragments shorter than    1690 bp

# BccI.SfeI (CCATC+CTRYAG) double digest, 9701 thousand fragments (6170927+3531009 sites), 307 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     253 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     390 bp
  40% of sequence in fragments shorter than     440 bp
  45% of sequence in fragments shorter than     489 bp
  50% of sequence in fragments shorter than     544 bp
  55% of sequence in fragments shorter than     604 bp
  60% of sequence in fragments shorter than     667 bp
  65% of sequence in fragments shorter than     735 bp
  70% of sequence in fragments shorter than     818 bp
  75% of sequence in fragments shorter than     911 bp
  80% of sequence in fragments shorter than    1028 bp
  85% of sequence in fragments shorter than    1176 bp
  90% of sequence in fragments shorter than    1380 bp
  95% of sequence in fragments shorter than    1739 bp

# Hpy188I.NcoI (TCNGA+CCATGG) double digest, 9701 thousand fragments (8942153+759594 sites), 307 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     200 bp
  20% of sequence in fragments shorter than     246 bp
  25% of sequence in fragments shorter than     293 bp
  30% of sequence in fragments shorter than     340 bp
  35% of sequence in fragments shorter than     382 bp
  40% of sequence in fragments shorter than     429 bp
  45% of sequence in fragments shorter than     480 bp
  50% of sequence in fragments shorter than     536 bp
  55% of sequence in fragments shorter than     592 bp
  60% of sequence in fragments shorter than     656 bp
  65% of sequence in fragments shorter than     727 bp
  70% of sequence in fragments shorter than     807 bp
  75% of sequence in fragments shorter than     900 bp
  80% of sequence in fragments shorter than    1012 bp
  85% of sequence in fragments shorter than    1154 bp
  90% of sequence in fragments shorter than    1338 bp
  95% of sequence in fragments shorter than    1670 bp

# AvaIII.TspRI (ATGCAT+CASTG) double digest, 9698 thousand fragments (933357+8765240 sites), 307 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     253 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     483 bp
  50% of sequence in fragments shorter than     535 bp
  55% of sequence in fragments shorter than     592 bp
  60% of sequence in fragments shorter than     654 bp
  65% of sequence in fragments shorter than     723 bp
  70% of sequence in fragments shorter than     801 bp
  75% of sequence in fragments shorter than     895 bp
  80% of sequence in fragments shorter than    1002 bp
  85% of sequence in fragments shorter than    1144 bp
  90% of sequence in fragments shorter than    1348 bp
  95% of sequence in fragments shorter than    1692 bp

# ApaLI.MboII (GTGCAC+GAAGA) double digest, 9695 thousand fragments (496255+9199496 sites), 307 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     307 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     401 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     620 bp
  60% of sequence in fragments shorter than     686 bp
  65% of sequence in fragments shorter than     758 bp
  70% of sequence in fragments shorter than     838 bp
  75% of sequence in fragments shorter than     932 bp
  80% of sequence in fragments shorter than    1042 bp
  85% of sequence in fragments shorter than    1186 bp
  90% of sequence in fragments shorter than    1389 bp
  95% of sequence in fragments shorter than    1731 bp

# StuI.TspRI (AGGCCT+CASTG) double digest, 9690 thousand fragments (925729+8765240 sites), 307 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     300 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     435 bp
  45% of sequence in fragments shorter than     487 bp
  50% of sequence in fragments shorter than     540 bp
  55% of sequence in fragments shorter than     597 bp
  60% of sequence in fragments shorter than     660 bp
  65% of sequence in fragments shorter than     729 bp
  70% of sequence in fragments shorter than     809 bp
  75% of sequence in fragments shorter than     905 bp
  80% of sequence in fragments shorter than    1012 bp
  85% of sequence in fragments shorter than    1154 bp
  90% of sequence in fragments shorter than    1360 bp
  95% of sequence in fragments shorter than    1697 bp

# BglII.HinfI (AGATCT+GANTC) double digest, 9690 thousand fragments (774732+8916054 sites), 307 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     338 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     420 bp
  45% of sequence in fragments shorter than     467 bp
  50% of sequence in fragments shorter than     517 bp
  55% of sequence in fragments shorter than     571 bp
  60% of sequence in fragments shorter than     630 bp
  65% of sequence in fragments shorter than     692 bp
  70% of sequence in fragments shorter than     763 bp
  75% of sequence in fragments shorter than     845 bp
  80% of sequence in fragments shorter than     946 bp
  85% of sequence in fragments shorter than    1069 bp
  90% of sequence in fragments shorter than    1243 bp
  95% of sequence in fragments shorter than    1536 bp

# BclI.Hpy188I (TGATCA+TCNGA) double digest, 9680 thousand fragments (738785+8942153 sites), 307 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     202 bp
  20% of sequence in fragments shorter than     248 bp
  25% of sequence in fragments shorter than     296 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     436 bp
  45% of sequence in fragments shorter than     487 bp
  50% of sequence in fragments shorter than     543 bp
  55% of sequence in fragments shorter than     600 bp
  60% of sequence in fragments shorter than     666 bp
  65% of sequence in fragments shorter than     738 bp
  70% of sequence in fragments shorter than     817 bp
  75% of sequence in fragments shorter than     910 bp
  80% of sequence in fragments shorter than    1021 bp
  85% of sequence in fragments shorter than    1154 bp
  90% of sequence in fragments shorter than    1342 bp
  95% of sequence in fragments shorter than    1670 bp

# FnuDII.Hpy188I (CGCG+TCNGA) double digest, 9675 thousand fragments (733940+8942153 sites), 307 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     388 bp
  40% of sequence in fragments shorter than     438 bp
  45% of sequence in fragments shorter than     490 bp
  50% of sequence in fragments shorter than     546 bp
  55% of sequence in fragments shorter than     604 bp
  60% of sequence in fragments shorter than     670 bp
  65% of sequence in fragments shorter than     742 bp
  70% of sequence in fragments shorter than     822 bp
  75% of sequence in fragments shorter than     916 bp
  80% of sequence in fragments shorter than    1027 bp
  85% of sequence in fragments shorter than    1167 bp
  90% of sequence in fragments shorter than    1347 bp
  95% of sequence in fragments shorter than    1670 bp

# HinfI.NcoI (GANTC+CCATGG) double digest, 9675 thousand fragments (8916054+759594 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     338 bp
  35% of sequence in fragments shorter than     375 bp
  40% of sequence in fragments shorter than     420 bp
  45% of sequence in fragments shorter than     467 bp
  50% of sequence in fragments shorter than     518 bp
  55% of sequence in fragments shorter than     570 bp
  60% of sequence in fragments shorter than     630 bp
  65% of sequence in fragments shorter than     692 bp
  70% of sequence in fragments shorter than     764 bp
  75% of sequence in fragments shorter than     847 bp
  80% of sequence in fragments shorter than     949 bp
  85% of sequence in fragments shorter than    1076 bp
  90% of sequence in fragments shorter than    1252 bp
  95% of sequence in fragments shorter than    1549 bp

# BsrI.MlyI (ACTGG+GAGTC) double digest, 9672 thousand fragments (5741310+3931692 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     300 bp
  30% of sequence in fragments shorter than     342 bp
  35% of sequence in fragments shorter than     387 bp
  40% of sequence in fragments shorter than     435 bp
  45% of sequence in fragments shorter than     483 bp
  50% of sequence in fragments shorter than     536 bp
  55% of sequence in fragments shorter than     593 bp
  60% of sequence in fragments shorter than     654 bp
  65% of sequence in fragments shorter than     719 bp
  70% of sequence in fragments shorter than     797 bp
  75% of sequence in fragments shorter than     885 bp
  80% of sequence in fragments shorter than     997 bp
  85% of sequence in fragments shorter than    1138 bp
  90% of sequence in fragments shorter than    1346 bp
  95% of sequence in fragments shorter than    1712 bp

# NdeI.TspRI (CATATG+CASTG) double digest, 9668 thousand fragments (903360+8765240 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     253 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     480 bp
  50% of sequence in fragments shorter than     531 bp
  55% of sequence in fragments shorter than     587 bp
  60% of sequence in fragments shorter than     648 bp
  65% of sequence in fragments shorter than     716 bp
  70% of sequence in fragments shorter than     793 bp
  75% of sequence in fragments shorter than     885 bp
  80% of sequence in fragments shorter than     998 bp
  85% of sequence in fragments shorter than    1141 bp
  90% of sequence in fragments shorter than    1346 bp
  95% of sequence in fragments shorter than    1691 bp

# AccI.HaeIII (GTMKAC+GGCC) double digest, 9662 thousand fragments (1101063+8562230 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     158 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     326 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     430 bp
  40% of sequence in fragments shorter than     490 bp
  45% of sequence in fragments shorter than     551 bp
  50% of sequence in fragments shorter than     619 bp
  55% of sequence in fragments shorter than     690 bp
  60% of sequence in fragments shorter than     771 bp
  65% of sequence in fragments shorter than     862 bp
  70% of sequence in fragments shorter than     963 bp
  75% of sequence in fragments shorter than    1086 bp
  80% of sequence in fragments shorter than    1235 bp
  85% of sequence in fragments shorter than    1420 bp
  90% of sequence in fragments shorter than    1686 bp
  95% of sequence in fragments shorter than    2145 bp

# BscAI.HphI (GCATC+GGTGA) double digest, 9659 thousand fragments (3652926+6007336 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     207 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     435 bp
  45% of sequence in fragments shorter than     485 bp
  50% of sequence in fragments shorter than     538 bp
  55% of sequence in fragments shorter than     595 bp
  60% of sequence in fragments shorter than     655 bp
  65% of sequence in fragments shorter than     722 bp
  70% of sequence in fragments shorter than     801 bp
  75% of sequence in fragments shorter than     892 bp
  80% of sequence in fragments shorter than    1006 bp
  85% of sequence in fragments shorter than    1152 bp
  90% of sequence in fragments shorter than    1351 bp
  95% of sequence in fragments shorter than    1691 bp

# ApaI.MboII (GGGCCC+GAAGA) double digest, 9659 thousand fragments (460339+9199496 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     259 bp
  25% of sequence in fragments shorter than     309 bp
  30% of sequence in fragments shorter than     353 bp
  35% of sequence in fragments shorter than     405 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     511 bp
  50% of sequence in fragments shorter than     568 bp
  55% of sequence in fragments shorter than     630 bp
  60% of sequence in fragments shorter than     698 bp
  65% of sequence in fragments shorter than     773 bp
  70% of sequence in fragments shorter than     856 bp
  75% of sequence in fragments shorter than     955 bp
  80% of sequence in fragments shorter than    1067 bp
  85% of sequence in fragments shorter than    1215 bp
  90% of sequence in fragments shorter than    1423 bp
  95% of sequence in fragments shorter than    1776 bp

# BspNCI.SspI (CCAGA+AATATT) double digest, 9659 thousand fragments (7282436+2377267 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     387 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     483 bp
  50% of sequence in fragments shorter than     534 bp
  55% of sequence in fragments shorter than     587 bp
  60% of sequence in fragments shorter than     647 bp
  65% of sequence in fragments shorter than     713 bp
  70% of sequence in fragments shorter than     787 bp
  75% of sequence in fragments shorter than     873 bp
  80% of sequence in fragments shorter than     979 bp
  85% of sequence in fragments shorter than    1109 bp
  90% of sequence in fragments shorter than    1286 bp
  95% of sequence in fragments shorter than    1611 bp

# BsaAI.Hpy188I (YACGTR+TCNGA) double digest, 9655 thousand fragments (713801+8942153 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     247 bp
  25% of sequence in fragments shorter than     294 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     484 bp
  50% of sequence in fragments shorter than     539 bp
  55% of sequence in fragments shorter than     596 bp
  60% of sequence in fragments shorter than     661 bp
  65% of sequence in fragments shorter than     731 bp
  70% of sequence in fragments shorter than     811 bp
  75% of sequence in fragments shorter than     903 bp
  80% of sequence in fragments shorter than    1014 bp
  85% of sequence in fragments shorter than    1154 bp
  90% of sequence in fragments shorter than    1334 bp
  95% of sequence in fragments shorter than    1660 bp

# BclI.HinfI (TGATCA+GANTC) double digest, 9654 thousand fragments (738785+8916054 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     298 bp
  30% of sequence in fragments shorter than     338 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     419 bp
  45% of sequence in fragments shorter than     466 bp
  50% of sequence in fragments shorter than     517 bp
  55% of sequence in fragments shorter than     570 bp
  60% of sequence in fragments shorter than     629 bp
  65% of sequence in fragments shorter than     690 bp
  70% of sequence in fragments shorter than     761 bp
  75% of sequence in fragments shorter than     844 bp
  80% of sequence in fragments shorter than     943 bp
  85% of sequence in fragments shorter than    1068 bp
  90% of sequence in fragments shorter than    1241 bp
  95% of sequence in fragments shorter than    1532 bp

# FnuDII.HinfI (CGCG+GANTC) double digest, 9649 thousand fragments (733940+8916054 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     302 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     382 bp
  40% of sequence in fragments shorter than     431 bp
  45% of sequence in fragments shorter than     482 bp
  50% of sequence in fragments shorter than     534 bp
  55% of sequence in fragments shorter than     593 bp
  60% of sequence in fragments shorter than     655 bp
  65% of sequence in fragments shorter than     719 bp
  70% of sequence in fragments shorter than     796 bp
  75% of sequence in fragments shorter than     884 bp
  80% of sequence in fragments shorter than     992 bp
  85% of sequence in fragments shorter than    1125 bp
  90% of sequence in fragments shorter than    1307 bp
  95% of sequence in fragments shorter than    1622 bp

# HaeIII.PpuMI (GGCC+RGGWCCY) double digest, 9646 thousand fragments (8562230+1085138 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than      90 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     275 bp
  25% of sequence in fragments shorter than     333 bp
  30% of sequence in fragments shorter than     383 bp
  35% of sequence in fragments shorter than     448 bp
  40% of sequence in fragments shorter than     516 bp
  45% of sequence in fragments shorter than     586 bp
  50% of sequence in fragments shorter than     667 bp
  55% of sequence in fragments shorter than     750 bp
  60% of sequence in fragments shorter than     848 bp
  65% of sequence in fragments shorter than     951 bp
  70% of sequence in fragments shorter than    1074 bp
  75% of sequence in fragments shorter than    1223 bp
  80% of sequence in fragments shorter than    1396 bp
  85% of sequence in fragments shorter than    1631 bp
  90% of sequence in fragments shorter than    1960 bp
  95% of sequence in fragments shorter than    2541 bp

# BceAI.Hpy188I (ACGGC+TCNGA) double digest, 9646 thousand fragments (705178+8942153 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     248 bp
  25% of sequence in fragments shorter than     295 bp
  30% of sequence in fragments shorter than     342 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     436 bp
  45% of sequence in fragments shorter than     488 bp
  50% of sequence in fragments shorter than     544 bp
  55% of sequence in fragments shorter than     602 bp
  60% of sequence in fragments shorter than     668 bp
  65% of sequence in fragments shorter than     741 bp
  70% of sequence in fragments shorter than     822 bp
  75% of sequence in fragments shorter than     915 bp
  80% of sequence in fragments shorter than    1028 bp
  85% of sequence in fragments shorter than    1171 bp
  90% of sequence in fragments shorter than    1355 bp
  95% of sequence in fragments shorter than    1686 bp

# HaeIII.PvuII (GGCC+CAGCTG) double digest, 9646 thousand fragments (8562230+1084260 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than      91 bp
  10% of sequence in fragments shorter than     155 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     335 bp
  30% of sequence in fragments shorter than     386 bp
  35% of sequence in fragments shorter than     448 bp
  40% of sequence in fragments shorter than     516 bp
  45% of sequence in fragments shorter than     585 bp
  50% of sequence in fragments shorter than     663 bp
  55% of sequence in fragments shorter than     744 bp
  60% of sequence in fragments shorter than     840 bp
  65% of sequence in fragments shorter than     942 bp
  70% of sequence in fragments shorter than    1063 bp
  75% of sequence in fragments shorter than    1209 bp
  80% of sequence in fragments shorter than    1380 bp
  85% of sequence in fragments shorter than    1609 bp
  90% of sequence in fragments shorter than    1928 bp
  95% of sequence in fragments shorter than    2501 bp

# EcoRV.MboII (GATATC+GAAGA) double digest, 9645 thousand fragments (446473+9199496 sites), 308 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     408 bp
  40% of sequence in fragments shorter than     460 bp
  45% of sequence in fragments shorter than     516 bp
  50% of sequence in fragments shorter than     572 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     704 bp
  65% of sequence in fragments shorter than     780 bp
  70% of sequence in fragments shorter than     865 bp
  75% of sequence in fragments shorter than     965 bp
  80% of sequence in fragments shorter than    1079 bp
  85% of sequence in fragments shorter than    1230 bp
  90% of sequence in fragments shorter than    1443 bp
  95% of sequence in fragments shorter than    1807 bp

# BciVI.Hpy188I (GTATCC+TCNGA) double digest, 9637 thousand fragments (696093+8942153 sites), 309 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     248 bp
  25% of sequence in fragments shorter than     295 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     385 bp
  40% of sequence in fragments shorter than     434 bp
  45% of sequence in fragments shorter than     486 bp
  50% of sequence in fragments shorter than     540 bp
  55% of sequence in fragments shorter than     597 bp
  60% of sequence in fragments shorter than     662 bp
  65% of sequence in fragments shorter than     731 bp
  70% of sequence in fragments shorter than     810 bp
  75% of sequence in fragments shorter than     901 bp
  80% of sequence in fragments shorter than    1010 bp
  85% of sequence in fragments shorter than    1149 bp
  90% of sequence in fragments shorter than    1342 bp
  95% of sequence in fragments shorter than    1672 bp

# BsaAI.HinfI (YACGTR+GANTC) double digest, 9629 thousand fragments (713801+8916054 sites), 309 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     340 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     422 bp
  45% of sequence in fragments shorter than     469 bp
  50% of sequence in fragments shorter than     520 bp
  55% of sequence in fragments shorter than     574 bp
  60% of sequence in fragments shorter than     634 bp
  65% of sequence in fragments shorter than     695 bp
  70% of sequence in fragments shorter than     767 bp
  75% of sequence in fragments shorter than     849 bp
  80% of sequence in fragments shorter than     950 bp
  85% of sequence in fragments shorter than    1076 bp
  90% of sequence in fragments shorter than    1251 bp
  95% of sequence in fragments shorter than    1547 bp

# HindIII.TspRI (AAGCTT+CASTG) double digest, 9625 thousand fragments (860365+8765240 sites), 309 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     300 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     389 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     486 bp
  50% of sequence in fragments shorter than     539 bp
  55% of sequence in fragments shorter than     596 bp
  60% of sequence in fragments shorter than     659 bp
  65% of sequence in fragments shorter than     726 bp
  70% of sequence in fragments shorter than     804 bp
  75% of sequence in fragments shorter than     896 bp
  80% of sequence in fragments shorter than    1003 bp
  85% of sequence in fragments shorter than    1141 bp
  90% of sequence in fragments shorter than    1347 bp
  95% of sequence in fragments shorter than    1713 bp

# BceAI.HinfI (ACGGC+GANTC) double digest, 9620 thousand fragments (705178+8916054 sites), 309 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     300 bp
  30% of sequence in fragments shorter than     340 bp
  35% of sequence in fragments shorter than     376 bp
  40% of sequence in fragments shorter than     423 bp
  45% of sequence in fragments shorter than     471 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     578 bp
  60% of sequence in fragments shorter than     638 bp
  65% of sequence in fragments shorter than     700 bp
  70% of sequence in fragments shorter than     774 bp
  75% of sequence in fragments shorter than     859 bp
  80% of sequence in fragments shorter than     962 bp
  85% of sequence in fragments shorter than    1091 bp
  90% of sequence in fragments shorter than    1269 bp
  95% of sequence in fragments shorter than    1573 bp

# TspRI.XbaI (CASTG+TCTAGA) double digest, 9615 thousand fragments (8765240+850998 sites), 309 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     390 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     487 bp
  50% of sequence in fragments shorter than     541 bp
  55% of sequence in fragments shorter than     598 bp
  60% of sequence in fragments shorter than     661 bp
  65% of sequence in fragments shorter than     730 bp
  70% of sequence in fragments shorter than     810 bp
  75% of sequence in fragments shorter than     899 bp
  80% of sequence in fragments shorter than    1005 bp
  85% of sequence in fragments shorter than    1139 bp
  90% of sequence in fragments shorter than    1336 bp
  95% of sequence in fragments shorter than    1673 bp

# EcoRI.TspRI (GAATTC+CASTG) double digest, 9612 thousand fragments (847401+8765240 sites), 310 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     389 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     488 bp
  50% of sequence in fragments shorter than     542 bp
  55% of sequence in fragments shorter than     598 bp
  60% of sequence in fragments shorter than     660 bp
  65% of sequence in fragments shorter than     728 bp
  70% of sequence in fragments shorter than     807 bp
  75% of sequence in fragments shorter than     900 bp
  80% of sequence in fragments shorter than    1002 bp
  85% of sequence in fragments shorter than    1143 bp
  90% of sequence in fragments shorter than    1345 bp
  95% of sequence in fragments shorter than    1683 bp

# BciVI.HinfI (GTATCC+GANTC) double digest, 9611 thousand fragments (696093+8916054 sites), 310 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     300 bp
  30% of sequence in fragments shorter than     340 bp
  35% of sequence in fragments shorter than     375 bp
  40% of sequence in fragments shorter than     422 bp
  45% of sequence in fragments shorter than     469 bp
  50% of sequence in fragments shorter than     520 bp
  55% of sequence in fragments shorter than     572 bp
  60% of sequence in fragments shorter than     631 bp
  65% of sequence in fragments shorter than     693 bp
  70% of sequence in fragments shorter than     765 bp
  75% of sequence in fragments shorter than     849 bp
  80% of sequence in fragments shorter than     950 bp
  85% of sequence in fragments shorter than    1075 bp
  90% of sequence in fragments shorter than    1247 bp
  95% of sequence in fragments shorter than    1538 bp

# BspNCI.HpaII (CCAGA+CCGG) double digest, 9599 thousand fragments (7282436+2317722 sites), 310 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     157 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     259 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     466 bp
  45% of sequence in fragments shorter than     522 bp
  50% of sequence in fragments shorter than     582 bp
  55% of sequence in fragments shorter than     646 bp
  60% of sequence in fragments shorter than     718 bp
  65% of sequence in fragments shorter than     797 bp
  70% of sequence in fragments shorter than     890 bp
  75% of sequence in fragments shorter than    1002 bp
  80% of sequence in fragments shorter than    1132 bp
  85% of sequence in fragments shorter than    1291 bp
  90% of sequence in fragments shorter than    1533 bp
  95% of sequence in fragments shorter than    1959 bp

# MboII.SpeI (GAAGA+ACTAGT) double digest, 9599 thousand fragments (9199496+400286 sites), 310 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     408 bp
  40% of sequence in fragments shorter than     460 bp
  45% of sequence in fragments shorter than     515 bp
  50% of sequence in fragments shorter than     572 bp
  55% of sequence in fragments shorter than     634 bp
  60% of sequence in fragments shorter than     702 bp
  65% of sequence in fragments shorter than     777 bp
  70% of sequence in fragments shorter than     862 bp
  75% of sequence in fragments shorter than     961 bp
  80% of sequence in fragments shorter than    1075 bp
  85% of sequence in fragments shorter than    1226 bp
  90% of sequence in fragments shorter than    1439 bp
  95% of sequence in fragments shorter than    1803 bp

# HpaI.MboII (GTTAAC+GAAGA) double digest, 9588 thousand fragments (389418+9199496 sites), 310 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     409 bp
  40% of sequence in fragments shorter than     460 bp
  45% of sequence in fragments shorter than     515 bp
  50% of sequence in fragments shorter than     572 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     703 bp
  65% of sequence in fragments shorter than     779 bp
  70% of sequence in fragments shorter than     864 bp
  75% of sequence in fragments shorter than     963 bp
  80% of sequence in fragments shorter than    1076 bp
  85% of sequence in fragments shorter than    1227 bp
  90% of sequence in fragments shorter than    1438 bp
  95% of sequence in fragments shorter than    1799 bp

# AflII.Hpy188I (CTTAAG+TCNGA) double digest, 9584 thousand fragments (643284+8942153 sites), 310 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     387 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     489 bp
  50% of sequence in fragments shorter than     545 bp
  55% of sequence in fragments shorter than     599 bp
  60% of sequence in fragments shorter than     665 bp
  65% of sequence in fragments shorter than     735 bp
  70% of sequence in fragments shorter than     815 bp
  75% of sequence in fragments shorter than     907 bp
  80% of sequence in fragments shorter than    1018 bp
  85% of sequence in fragments shorter than    1159 bp
  90% of sequence in fragments shorter than    1352 bp
  95% of sequence in fragments shorter than    1686 bp

# BspKT6I.SspI (GATC+AATATT) double digest, 9576 thousand fragments (7199434+2377267 sites), 311 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     197 bp
  20% of sequence in fragments shorter than     245 bp
  25% of sequence in fragments shorter than     290 bp
  30% of sequence in fragments shorter than     331 bp
  35% of sequence in fragments shorter than     374 bp
  40% of sequence in fragments shorter than     419 bp
  45% of sequence in fragments shorter than     465 bp
  50% of sequence in fragments shorter than     514 bp
  55% of sequence in fragments shorter than     568 bp
  60% of sequence in fragments shorter than     627 bp
  65% of sequence in fragments shorter than     692 bp
  70% of sequence in fragments shorter than     767 bp
  75% of sequence in fragments shorter than     854 bp
  80% of sequence in fragments shorter than     957 bp
  85% of sequence in fragments shorter than    1083 bp
  90% of sequence in fragments shorter than    1276 bp
  95% of sequence in fragments shorter than    1627 bp

# MboII.SapI (GAAGA+GCTCTTC) double digest, 9576 thousand fragments (9199496+377161 sites), 311 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     374 bp
  35% of sequence in fragments shorter than     428 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     600 bp
  55% of sequence in fragments shorter than     666 bp
  60% of sequence in fragments shorter than     738 bp
  65% of sequence in fragments shorter than     817 bp
  70% of sequence in fragments shorter than     906 bp
  75% of sequence in fragments shorter than    1010 bp
  80% of sequence in fragments shorter than    1128 bp
  85% of sequence in fragments shorter than    1288 bp
  90% of sequence in fragments shorter than    1509 bp
  95% of sequence in fragments shorter than    1888 bp

# MboII.SmaI (GAAGA+CCCGGG) double digest, 9575 thousand fragments (9199496+376940 sites), 311 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     262 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     354 bp
  35% of sequence in fragments shorter than     405 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     508 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     690 bp
  65% of sequence in fragments shorter than     763 bp
  70% of sequence in fragments shorter than     845 bp
  75% of sequence in fragments shorter than     939 bp
  80% of sequence in fragments shorter than    1047 bp
  85% of sequence in fragments shorter than    1188 bp
  90% of sequence in fragments shorter than    1386 bp
  95% of sequence in fragments shorter than    1719 bp

# HaeII.Hpy188I (RGCGCY+TCNGA) double digest, 9571 thousand fragments (630101+8942153 sites), 311 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     389 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     490 bp
  50% of sequence in fragments shorter than     546 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     671 bp
  65% of sequence in fragments shorter than     744 bp
  70% of sequence in fragments shorter than     826 bp
  75% of sequence in fragments shorter than     920 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1174 bp
  90% of sequence in fragments shorter than    1356 bp
  95% of sequence in fragments shorter than    1683 bp

# AvaI.HaeIII (CYCGRG+GGCC) double digest, 9567 thousand fragments (1005714+8562230 sites), 311 bp average fragment length
   5% of sequence in fragments shorter than      91 bp
  10% of sequence in fragments shorter than     154 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     340 bp
  30% of sequence in fragments shorter than     395 bp
  35% of sequence in fragments shorter than     461 bp
  40% of sequence in fragments shorter than     531 bp
  45% of sequence in fragments shorter than     609 bp
  50% of sequence in fragments shorter than     692 bp
  55% of sequence in fragments shorter than     786 bp
  60% of sequence in fragments shorter than     884 bp
  65% of sequence in fragments shorter than    1002 bp
  70% of sequence in fragments shorter than    1135 bp
  75% of sequence in fragments shorter than    1286 bp
  80% of sequence in fragments shorter than    1478 bp
  85% of sequence in fragments shorter than    1727 bp
  90% of sequence in fragments shorter than    2080 bp
  95% of sequence in fragments shorter than    2704 bp

# BamHI.MboII (GGATCC+GAAGA) double digest, 9564 thousand fragments (365999+9199496 sites), 311 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     262 bp
  25% of sequence in fragments shorter than     312 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     409 bp
  40% of sequence in fragments shorter than     462 bp
  45% of sequence in fragments shorter than     517 bp
  50% of sequence in fragments shorter than     574 bp
  55% of sequence in fragments shorter than     638 bp
  60% of sequence in fragments shorter than     706 bp
  65% of sequence in fragments shorter than     782 bp
  70% of sequence in fragments shorter than     867 bp
  75% of sequence in fragments shorter than     966 bp
  80% of sequence in fragments shorter than    1079 bp
  85% of sequence in fragments shorter than    1229 bp
  90% of sequence in fragments shorter than    1439 bp
  95% of sequence in fragments shorter than    1798 bp

# AflII.HinfI (CTTAAG+GANTC) double digest, 9558 thousand fragments (643284+8916054 sites), 311 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     377 bp
  40% of sequence in fragments shorter than     424 bp
  45% of sequence in fragments shorter than     472 bp
  50% of sequence in fragments shorter than     522 bp
  55% of sequence in fragments shorter than     576 bp
  60% of sequence in fragments shorter than     636 bp
  65% of sequence in fragments shorter than     698 bp
  70% of sequence in fragments shorter than     771 bp
  75% of sequence in fragments shorter than     856 bp
  80% of sequence in fragments shorter than     958 bp
  85% of sequence in fragments shorter than    1085 bp
  90% of sequence in fragments shorter than    1260 bp
  95% of sequence in fragments shorter than    1555 bp

# HaeII.HinfI (RGCGCY+GANTC) double digest, 9545 thousand fragments (630101+8916054 sites), 312 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     341 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     430 bp
  45% of sequence in fragments shorter than     479 bp
  50% of sequence in fragments shorter than     531 bp
  55% of sequence in fragments shorter than     588 bp
  60% of sequence in fragments shorter than     650 bp
  65% of sequence in fragments shorter than     714 bp
  70% of sequence in fragments shorter than     790 bp
  75% of sequence in fragments shorter than     877 bp
  80% of sequence in fragments shorter than     984 bp
  85% of sequence in fragments shorter than    1116 bp
  90% of sequence in fragments shorter than    1300 bp
  95% of sequence in fragments shorter than    1613 bp

# Hpy188I.SacI (TCNGA+GAGCTC) double digest, 9544 thousand fragments (8942153+603323 sites), 312 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     252 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     391 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     495 bp
  50% of sequence in fragments shorter than     551 bp
  55% of sequence in fragments shorter than     612 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     753 bp
  70% of sequence in fragments shorter than     835 bp
  75% of sequence in fragments shorter than     931 bp
  80% of sequence in fragments shorter than    1045 bp
  85% of sequence in fragments shorter than    1187 bp
  90% of sequence in fragments shorter than    1376 bp
  95% of sequence in fragments shorter than    1712 bp

# BspHI.HaeIII (TCATGA+GGCC) double digest, 9540 thousand fragments (978290+8562230 sites), 312 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     332 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     440 bp
  40% of sequence in fragments shorter than     504 bp
  45% of sequence in fragments shorter than     568 bp
  50% of sequence in fragments shorter than     640 bp
  55% of sequence in fragments shorter than     718 bp
  60% of sequence in fragments shorter than     806 bp
  65% of sequence in fragments shorter than     898 bp
  70% of sequence in fragments shorter than    1010 bp
  75% of sequence in fragments shorter than    1139 bp
  80% of sequence in fragments shorter than    1292 bp
  85% of sequence in fragments shorter than    1496 bp
  90% of sequence in fragments shorter than    1780 bp
  95% of sequence in fragments shorter than    2277 bp

# BglII.TspRI (AGATCT+CASTG) double digest, 9539 thousand fragments (774732+8765240 sites), 312 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     302 bp
  30% of sequence in fragments shorter than     346 bp
  35% of sequence in fragments shorter than     393 bp
  40% of sequence in fragments shorter than     441 bp
  45% of sequence in fragments shorter than     493 bp
  50% of sequence in fragments shorter than     547 bp
  55% of sequence in fragments shorter than     606 bp
  60% of sequence in fragments shorter than     670 bp
  65% of sequence in fragments shorter than     741 bp
  70% of sequence in fragments shorter than     823 bp
  75% of sequence in fragments shorter than     921 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1177 bp
  90% of sequence in fragments shorter than    1386 bp
  95% of sequence in fragments shorter than    1756 bp

# HphI.SfeI (GGTGA+CTRYAG) double digest, 9537 thousand fragments (6007336+3531009 sites), 312 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     160 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     302 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     393 bp
  40% of sequence in fragments shorter than     443 bp
  45% of sequence in fragments shorter than     492 bp
  50% of sequence in fragments shorter than     546 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     665 bp
  65% of sequence in fragments shorter than     732 bp
  70% of sequence in fragments shorter than     810 bp
  75% of sequence in fragments shorter than     904 bp
  80% of sequence in fragments shorter than    1020 bp
  85% of sequence in fragments shorter than    1165 bp
  90% of sequence in fragments shorter than    1368 bp
  95% of sequence in fragments shorter than    1715 bp

# AvrII.Hpy188I (CCTAGG+TCNGA) double digest, 9536 thousand fragments (594956+8942153 sites), 312 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     203 bp
  20% of sequence in fragments shorter than     249 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     390 bp
  40% of sequence in fragments shorter than     438 bp
  45% of sequence in fragments shorter than     490 bp
  50% of sequence in fragments shorter than     546 bp
  55% of sequence in fragments shorter than     603 bp
  60% of sequence in fragments shorter than     669 bp
  65% of sequence in fragments shorter than     742 bp
  70% of sequence in fragments shorter than     821 bp
  75% of sequence in fragments shorter than     911 bp
  80% of sequence in fragments shorter than    1022 bp
  85% of sequence in fragments shorter than    1161 bp
  90% of sequence in fragments shorter than    1354 bp
  95% of sequence in fragments shorter than    1684 bp

# BstEII.MboII (GGTNACC+GAAGA) double digest, 9534 thousand fragments (335865+9199496 sites), 312 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     263 bp
  25% of sequence in fragments shorter than     314 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     463 bp
  45% of sequence in fragments shorter than     518 bp
  50% of sequence in fragments shorter than     576 bp
  55% of sequence in fragments shorter than     639 bp
  60% of sequence in fragments shorter than     708 bp
  65% of sequence in fragments shorter than     785 bp
  70% of sequence in fragments shorter than     870 bp
  75% of sequence in fragments shorter than     970 bp
  80% of sequence in fragments shorter than    1083 bp
  85% of sequence in fragments shorter than    1235 bp
  90% of sequence in fragments shorter than    1446 bp
  95% of sequence in fragments shorter than    1806 bp

# NcoI.TspRI (CCATGG+CASTG) double digest, 9524 thousand fragments (759594+8765240 sites), 312 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     302 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     396 bp
  40% of sequence in fragments shorter than     446 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     615 bp
  60% of sequence in fragments shorter than     681 bp
  65% of sequence in fragments shorter than     755 bp
  70% of sequence in fragments shorter than     840 bp
  75% of sequence in fragments shorter than     940 bp
  80% of sequence in fragments shorter than    1054 bp
  85% of sequence in fragments shorter than    1207 bp
  90% of sequence in fragments shorter than    1417 bp
  95% of sequence in fragments shorter than    1799 bp

# HinfI.SacI (GANTC+GAGCTC) double digest, 9518 thousand fragments (8916054+603323 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     342 bp
  35% of sequence in fragments shorter than     378 bp
  40% of sequence in fragments shorter than     426 bp
  45% of sequence in fragments shorter than     474 bp
  50% of sequence in fragments shorter than     526 bp
  55% of sequence in fragments shorter than     583 bp
  60% of sequence in fragments shorter than     643 bp
  65% of sequence in fragments shorter than     706 bp
  70% of sequence in fragments shorter than     780 bp
  75% of sequence in fragments shorter than     866 bp
  80% of sequence in fragments shorter than     970 bp
  85% of sequence in fragments shorter than    1099 bp
  90% of sequence in fragments shorter than    1277 bp
  95% of sequence in fragments shorter than    1583 bp

# BspKT6I.HpaII (GATC+CCGG) double digest, 9516 thousand fragments (7199434+2317722 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     159 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     259 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     464 bp
  45% of sequence in fragments shorter than     519 bp
  50% of sequence in fragments shorter than     580 bp
  55% of sequence in fragments shorter than     645 bp
  60% of sequence in fragments shorter than     716 bp
  65% of sequence in fragments shorter than     796 bp
  70% of sequence in fragments shorter than     886 bp
  75% of sequence in fragments shorter than     993 bp
  80% of sequence in fragments shorter than    1113 bp
  85% of sequence in fragments shorter than    1276 bp
  90% of sequence in fragments shorter than    1505 bp
  95% of sequence in fragments shorter than    1907 bp

# HgaI.Hpy188I (GACGC+TCNGA) double digest, 9513 thousand fragments (571889+8942153 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     252 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     393 bp
  40% of sequence in fragments shorter than     444 bp
  45% of sequence in fragments shorter than     496 bp
  50% of sequence in fragments shorter than     553 bp
  55% of sequence in fragments shorter than     613 bp
  60% of sequence in fragments shorter than     680 bp
  65% of sequence in fragments shorter than     754 bp
  70% of sequence in fragments shorter than     836 bp
  75% of sequence in fragments shorter than     932 bp
  80% of sequence in fragments shorter than    1046 bp
  85% of sequence in fragments shorter than    1189 bp
  90% of sequence in fragments shorter than    1378 bp
  95% of sequence in fragments shorter than    1715 bp

# EciI.Hpy188I (GGCGGA+TCNGA) double digest, 9512 thousand fragments (571273+8942153 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     204 bp
  20% of sequence in fragments shorter than     250 bp
  25% of sequence in fragments shorter than     297 bp
  30% of sequence in fragments shorter than     342 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     433 bp
  45% of sequence in fragments shorter than     482 bp
  50% of sequence in fragments shorter than     537 bp
  55% of sequence in fragments shorter than     593 bp
  60% of sequence in fragments shorter than     656 bp
  65% of sequence in fragments shorter than     726 bp
  70% of sequence in fragments shorter than     805 bp
  75% of sequence in fragments shorter than     894 bp
  80% of sequence in fragments shorter than    1002 bp
  85% of sequence in fragments shorter than    1138 bp
  90% of sequence in fragments shorter than    1310 bp
  95% of sequence in fragments shorter than    1622 bp

# AvrII.HinfI (CCTAGG+GANTC) double digest, 9510 thousand fragments (594956+8916054 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     303 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     382 bp
  40% of sequence in fragments shorter than     430 bp
  45% of sequence in fragments shorter than     478 bp
  50% of sequence in fragments shorter than     529 bp
  55% of sequence in fragments shorter than     585 bp
  60% of sequence in fragments shorter than     646 bp
  65% of sequence in fragments shorter than     708 bp
  70% of sequence in fragments shorter than     783 bp
  75% of sequence in fragments shorter than     869 bp
  80% of sequence in fragments shorter than     974 bp
  85% of sequence in fragments shorter than    1104 bp
  90% of sequence in fragments shorter than    1281 bp
  95% of sequence in fragments shorter than    1587 bp

# Hpy188I.MfeI (TCNGA+CAATTG) double digest, 9505 thousand fragments (8942153+564303 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     251 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     391 bp
  40% of sequence in fragments shorter than     441 bp
  45% of sequence in fragments shorter than     492 bp
  50% of sequence in fragments shorter than     548 bp
  55% of sequence in fragments shorter than     606 bp
  60% of sequence in fragments shorter than     673 bp
  65% of sequence in fragments shorter than     745 bp
  70% of sequence in fragments shorter than     823 bp
  75% of sequence in fragments shorter than     917 bp
  80% of sequence in fragments shorter than    1029 bp
  85% of sequence in fragments shorter than    1170 bp
  90% of sequence in fragments shorter than    1365 bp
  95% of sequence in fragments shorter than    1699 bp

# BclI.TspRI (TGATCA+CASTG) double digest, 9503 thousand fragments (738785+8765240 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     307 bp
  30% of sequence in fragments shorter than     352 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     448 bp
  45% of sequence in fragments shorter than     500 bp
  50% of sequence in fragments shorter than     555 bp
  55% of sequence in fragments shorter than     615 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     749 bp
  70% of sequence in fragments shorter than     830 bp
  75% of sequence in fragments shorter than     925 bp
  80% of sequence in fragments shorter than    1033 bp
  85% of sequence in fragments shorter than    1179 bp
  90% of sequence in fragments shorter than    1387 bp
  95% of sequence in fragments shorter than    1759 bp

# FnuDII.TspRI (CGCG+CASTG) double digest, 9498 thousand fragments (733940+8765240 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     309 bp
  30% of sequence in fragments shorter than     357 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     460 bp
  45% of sequence in fragments shorter than     516 bp
  50% of sequence in fragments shorter than     575 bp
  55% of sequence in fragments shorter than     639 bp
  60% of sequence in fragments shorter than     708 bp
  65% of sequence in fragments shorter than     787 bp
  70% of sequence in fragments shorter than     877 bp
  75% of sequence in fragments shorter than     977 bp
  80% of sequence in fragments shorter than    1101 bp
  85% of sequence in fragments shorter than    1265 bp
  90% of sequence in fragments shorter than    1493 bp
  95% of sequence in fragments shorter than    1903 bp

# MboII.SexAI (GAAGA+ACCWGGT) double digest, 9496 thousand fragments (9199496+297900 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     413 bp
  40% of sequence in fragments shorter than     465 bp
  45% of sequence in fragments shorter than     519 bp
  50% of sequence in fragments shorter than     578 bp
  55% of sequence in fragments shorter than     641 bp
  60% of sequence in fragments shorter than     711 bp
  65% of sequence in fragments shorter than     787 bp
  70% of sequence in fragments shorter than     873 bp
  75% of sequence in fragments shorter than     973 bp
  80% of sequence in fragments shorter than    1087 bp
  85% of sequence in fragments shorter than    1239 bp
  90% of sequence in fragments shorter than    1451 bp
  95% of sequence in fragments shorter than    1812 bp

# ApoI.StyI (RAATTY+CCWWGG) double digest, 9496 thousand fragments (6382433+3114107 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     391 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     487 bp
  50% of sequence in fragments shorter than     535 bp
  55% of sequence in fragments shorter than     591 bp
  60% of sequence in fragments shorter than     652 bp
  65% of sequence in fragments shorter than     719 bp
  70% of sequence in fragments shorter than     797 bp
  75% of sequence in fragments shorter than     885 bp
  80% of sequence in fragments shorter than     992 bp
  85% of sequence in fragments shorter than    1131 bp
  90% of sequence in fragments shorter than    1326 bp
  95% of sequence in fragments shorter than    1657 bp

# AciI.BbvI (CCGC+GCAGC) double digest, 9495 thousand fragments (4206130+5290051 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     240 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     377 bp
  30% of sequence in fragments shorter than     450 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     606 bp
  45% of sequence in fragments shorter than     690 bp
  50% of sequence in fragments shorter than     783 bp
  55% of sequence in fragments shorter than     877 bp
  60% of sequence in fragments shorter than     986 bp
  65% of sequence in fragments shorter than    1106 bp
  70% of sequence in fragments shorter than    1250 bp
  75% of sequence in fragments shorter than    1417 bp
  80% of sequence in fragments shorter than    1622 bp
  85% of sequence in fragments shorter than    1886 bp
  90% of sequence in fragments shorter than    2255 bp
  95% of sequence in fragments shorter than    2905 bp

# AvaIII.HaeIII (ATGCAT+GGCC) double digest, 9495 thousand fragments (933357+8562230 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     333 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     438 bp
  40% of sequence in fragments shorter than     500 bp
  45% of sequence in fragments shorter than     563 bp
  50% of sequence in fragments shorter than     632 bp
  55% of sequence in fragments shorter than     706 bp
  60% of sequence in fragments shorter than     792 bp
  65% of sequence in fragments shorter than     881 bp
  70% of sequence in fragments shorter than     989 bp
  75% of sequence in fragments shorter than    1114 bp
  80% of sequence in fragments shorter than    1266 bp
  85% of sequence in fragments shorter than    1455 bp
  90% of sequence in fragments shorter than    1730 bp
  95% of sequence in fragments shorter than    2203 bp

# Hpy188I.SphI (TCNGA+GCATGC) double digest, 9492 thousand fragments (8942153+550952 sites), 313 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     251 bp
  25% of sequence in fragments shorter than     299 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     389 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     490 bp
  50% of sequence in fragments shorter than     546 bp
  55% of sequence in fragments shorter than     603 bp
  60% of sequence in fragments shorter than     669 bp
  65% of sequence in fragments shorter than     741 bp
  70% of sequence in fragments shorter than     821 bp
  75% of sequence in fragments shorter than     914 bp
  80% of sequence in fragments shorter than    1025 bp
  85% of sequence in fragments shorter than    1165 bp
  90% of sequence in fragments shorter than    1346 bp
  95% of sequence in fragments shorter than    1673 bp

# HaeIII.StuI (GGCC+AGGCCT) double digest, 9487 thousand fragments (8562230+925729 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     581 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     847 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1206 bp
  75% of sequence in fragments shorter than    1363 bp
  80% of sequence in fragments shorter than    1563 bp
  85% of sequence in fragments shorter than    1822 bp
  90% of sequence in fragments shorter than    2190 bp
  95% of sequence in fragments shorter than    2838 bp

# HgaI.HinfI (GACGC+GANTC) double digest, 9487 thousand fragments (571889+8916054 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     259 bp
  25% of sequence in fragments shorter than     304 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     382 bp
  40% of sequence in fragments shorter than     430 bp
  45% of sequence in fragments shorter than     479 bp
  50% of sequence in fragments shorter than     530 bp
  55% of sequence in fragments shorter than     587 bp
  60% of sequence in fragments shorter than     649 bp
  65% of sequence in fragments shorter than     711 bp
  70% of sequence in fragments shorter than     787 bp
  75% of sequence in fragments shorter than     873 bp
  80% of sequence in fragments shorter than     978 bp
  85% of sequence in fragments shorter than    1109 bp
  90% of sequence in fragments shorter than    1289 bp
  95% of sequence in fragments shorter than    1597 bp

# KpnI.MboII (GGTACC+GAAGA) double digest, 9486 thousand fragments (287974+9199496 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     314 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     413 bp
  40% of sequence in fragments shorter than     466 bp
  45% of sequence in fragments shorter than     522 bp
  50% of sequence in fragments shorter than     581 bp
  55% of sequence in fragments shorter than     645 bp
  60% of sequence in fragments shorter than     714 bp
  65% of sequence in fragments shorter than     792 bp
  70% of sequence in fragments shorter than     877 bp
  75% of sequence in fragments shorter than     975 bp
  80% of sequence in fragments shorter than    1094 bp
  85% of sequence in fragments shorter than    1248 bp
  90% of sequence in fragments shorter than    1463 bp
  95% of sequence in fragments shorter than    1829 bp

# EciI.HinfI (GGCGGA+GANTC) double digest, 9486 thousand fragments (571273+8916054 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     304 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     434 bp
  45% of sequence in fragments shorter than     484 bp
  50% of sequence in fragments shorter than     537 bp
  55% of sequence in fragments shorter than     595 bp
  60% of sequence in fragments shorter than     658 bp
  65% of sequence in fragments shorter than     722 bp
  70% of sequence in fragments shorter than     798 bp
  75% of sequence in fragments shorter than     887 bp
  80% of sequence in fragments shorter than     994 bp
  85% of sequence in fragments shorter than    1128 bp
  90% of sequence in fragments shorter than    1309 bp
  95% of sequence in fragments shorter than    1625 bp

# MboII.NheI (GAAGA+GCTAGC) double digest, 9486 thousand fragments (9199496+287384 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     466 bp
  45% of sequence in fragments shorter than     521 bp
  50% of sequence in fragments shorter than     581 bp
  55% of sequence in fragments shorter than     646 bp
  60% of sequence in fragments shorter than     715 bp
  65% of sequence in fragments shorter than     792 bp
  70% of sequence in fragments shorter than     879 bp
  75% of sequence in fragments shorter than     981 bp
  80% of sequence in fragments shorter than    1097 bp
  85% of sequence in fragments shorter than    1252 bp
  90% of sequence in fragments shorter than    1467 bp
  95% of sequence in fragments shorter than    1835 bp

# ApoI.NspI (RAATTY+RCATGY) double digest, 9486 thousand fragments (6382433+3104359 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     396 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     497 bp
  50% of sequence in fragments shorter than     555 bp
  55% of sequence in fragments shorter than     617 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     760 bp
  70% of sequence in fragments shorter than     848 bp
  75% of sequence in fragments shorter than     951 bp
  80% of sequence in fragments shorter than    1079 bp
  85% of sequence in fragments shorter than    1247 bp
  90% of sequence in fragments shorter than    1475 bp
  95% of sequence in fragments shorter than    1929 bp

# Hpy188I.ScaI (TCNGA+AGTACT) double digest, 9485 thousand fragments (8942153+543793 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     205 bp
  20% of sequence in fragments shorter than     252 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     494 bp
  50% of sequence in fragments shorter than     550 bp
  55% of sequence in fragments shorter than     609 bp
  60% of sequence in fragments shorter than     675 bp
  65% of sequence in fragments shorter than     748 bp
  70% of sequence in fragments shorter than     829 bp
  75% of sequence in fragments shorter than     923 bp
  80% of sequence in fragments shorter than    1036 bp
  85% of sequence in fragments shorter than    1179 bp
  90% of sequence in fragments shorter than    1363 bp
  95% of sequence in fragments shorter than    1695 bp

# HinfI.MfeI (GANTC+CAATTG) double digest, 9479 thousand fragments (8916054+564303 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     304 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     382 bp
  40% of sequence in fragments shorter than     429 bp
  45% of sequence in fragments shorter than     477 bp
  50% of sequence in fragments shorter than     528 bp
  55% of sequence in fragments shorter than     584 bp
  60% of sequence in fragments shorter than     642 bp
  65% of sequence in fragments shorter than     705 bp
  70% of sequence in fragments shorter than     779 bp
  75% of sequence in fragments shorter than     863 bp
  80% of sequence in fragments shorter than     966 bp
  85% of sequence in fragments shorter than    1095 bp
  90% of sequence in fragments shorter than    1269 bp
  95% of sequence in fragments shorter than    1566 bp

# BsaAI.TspRI (YACGTR+CASTG) double digest, 9478 thousand fragments (713801+8765240 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     259 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     398 bp
  40% of sequence in fragments shorter than     447 bp
  45% of sequence in fragments shorter than     499 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     615 bp
  60% of sequence in fragments shorter than     680 bp
  65% of sequence in fragments shorter than     753 bp
  70% of sequence in fragments shorter than     837 bp
  75% of sequence in fragments shorter than     934 bp
  80% of sequence in fragments shorter than    1042 bp
  85% of sequence in fragments shorter than    1190 bp
  90% of sequence in fragments shorter than    1397 bp
  95% of sequence in fragments shorter than    1764 bp

# BceAI.TspRI (ACGGC+CASTG) double digest, 9469 thousand fragments (705178+8765240 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     259 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     352 bp
  35% of sequence in fragments shorter than     401 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     561 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     690 bp
  65% of sequence in fragments shorter than     765 bp
  70% of sequence in fragments shorter than     852 bp
  75% of sequence in fragments shorter than     949 bp
  80% of sequence in fragments shorter than    1068 bp
  85% of sequence in fragments shorter than    1225 bp
  90% of sequence in fragments shorter than    1443 bp
  95% of sequence in fragments shorter than    1837 bp

# HinfI.SphI (GANTC+GCATGC) double digest, 9466 thousand fragments (8916054+550952 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     344 bp
  35% of sequence in fragments shorter than     382 bp
  40% of sequence in fragments shorter than     430 bp
  45% of sequence in fragments shorter than     478 bp
  50% of sequence in fragments shorter than     529 bp
  55% of sequence in fragments shorter than     586 bp
  60% of sequence in fragments shorter than     647 bp
  65% of sequence in fragments shorter than     709 bp
  70% of sequence in fragments shorter than     783 bp
  75% of sequence in fragments shorter than     868 bp
  80% of sequence in fragments shorter than     972 bp
  85% of sequence in fragments shorter than    1101 bp
  90% of sequence in fragments shorter than    1278 bp
  95% of sequence in fragments shorter than    1581 bp

# HaeIII.NdeI (GGCC+CATATG) double digest, 9465 thousand fragments (8562230+903360 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     334 bp
  30% of sequence in fragments shorter than     382 bp
  35% of sequence in fragments shorter than     440 bp
  40% of sequence in fragments shorter than     500 bp
  45% of sequence in fragments shorter than     563 bp
  50% of sequence in fragments shorter than     634 bp
  55% of sequence in fragments shorter than     706 bp
  60% of sequence in fragments shorter than     791 bp
  65% of sequence in fragments shorter than     884 bp
  70% of sequence in fragments shorter than     993 bp
  75% of sequence in fragments shorter than    1115 bp
  80% of sequence in fragments shorter than    1267 bp
  85% of sequence in fragments shorter than    1458 bp
  90% of sequence in fragments shorter than    1729 bp
  95% of sequence in fragments shorter than    2197 bp

# BciVI.TspRI (GTATCC+CASTG) double digest, 9460 thousand fragments (696093+8765240 sites), 314 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     398 bp
  40% of sequence in fragments shorter than     447 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     553 bp
  55% of sequence in fragments shorter than     612 bp
  60% of sequence in fragments shorter than     677 bp
  65% of sequence in fragments shorter than     749 bp
  70% of sequence in fragments shorter than     832 bp
  75% of sequence in fragments shorter than     926 bp
  80% of sequence in fragments shorter than    1037 bp
  85% of sequence in fragments shorter than    1184 bp
  90% of sequence in fragments shorter than    1380 bp
  95% of sequence in fragments shorter than    1759 bp

# HinfI.ScaI (GANTC+AGTACT) double digest, 9459 thousand fragments (8916054+543793 sites), 315 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     304 bp
  30% of sequence in fragments shorter than     343 bp
  35% of sequence in fragments shorter than     381 bp
  40% of sequence in fragments shorter than     428 bp
  45% of sequence in fragments shorter than     477 bp
  50% of sequence in fragments shorter than     527 bp
  55% of sequence in fragments shorter than     583 bp
  60% of sequence in fragments shorter than     643 bp
  65% of sequence in fragments shorter than     705 bp
  70% of sequence in fragments shorter than     778 bp
  75% of sequence in fragments shorter than     862 bp
  80% of sequence in fragments shorter than     965 bp
  85% of sequence in fragments shorter than    1092 bp
  90% of sequence in fragments shorter than    1265 bp
  95% of sequence in fragments shorter than    1562 bp

# ApaLI.Hpy188I (GTGCAC+TCNGA) double digest, 9437 thousand fragments (496255+8942153 sites), 315 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     253 bp
  25% of sequence in fragments shorter than     301 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     392 bp
  40% of sequence in fragments shorter than     443 bp
  45% of sequence in fragments shorter than     495 bp
  50% of sequence in fragments shorter than     551 bp
  55% of sequence in fragments shorter than     609 bp
  60% of sequence in fragments shorter than     675 bp
  65% of sequence in fragments shorter than     743 bp
  70% of sequence in fragments shorter than     824 bp
  75% of sequence in fragments shorter than     918 bp
  80% of sequence in fragments shorter than    1031 bp
  85% of sequence in fragments shorter than    1173 bp
  90% of sequence in fragments shorter than    1357 bp
  95% of sequence in fragments shorter than    1687 bp

# Hin4II.SspI (CCTTC+AATATT) double digest, 9436 thousand fragments (7059915+2377267 sites), 315 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     317 bp
  30% of sequence in fragments shorter than     362 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     461 bp
  45% of sequence in fragments shorter than     514 bp
  50% of sequence in fragments shorter than     566 bp
  55% of sequence in fragments shorter than     626 bp
  60% of sequence in fragments shorter than     690 bp
  65% of sequence in fragments shorter than     762 bp
  70% of sequence in fragments shorter than     845 bp
  75% of sequence in fragments shorter than     937 bp
  80% of sequence in fragments shorter than    1045 bp
  85% of sequence in fragments shorter than    1191 bp
  90% of sequence in fragments shorter than    1389 bp
  95% of sequence in fragments shorter than    1729 bp

# BsrBI.MboII (CCGCTC+GAAGA) double digest, 9434 thousand fragments (235523+9199496 sites), 315 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     317 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     416 bp
  40% of sequence in fragments shorter than     468 bp
  45% of sequence in fragments shorter than     522 bp
  50% of sequence in fragments shorter than     581 bp
  55% of sequence in fragments shorter than     645 bp
  60% of sequence in fragments shorter than     714 bp
  65% of sequence in fragments shorter than     791 bp
  70% of sequence in fragments shorter than     877 bp
  75% of sequence in fragments shorter than     978 bp
  80% of sequence in fragments shorter than    1092 bp
  85% of sequence in fragments shorter than    1244 bp
  90% of sequence in fragments shorter than    1456 bp
  95% of sequence in fragments shorter than    1817 bp

# KasI.MboII (GGCGCC+GAAGA) double digest, 9429 thousand fragments (230381+9199496 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     361 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     464 bp
  45% of sequence in fragments shorter than     518 bp
  50% of sequence in fragments shorter than     576 bp
  55% of sequence in fragments shorter than     638 bp
  60% of sequence in fragments shorter than     706 bp
  65% of sequence in fragments shorter than     782 bp
  70% of sequence in fragments shorter than     865 bp
  75% of sequence in fragments shorter than     963 bp
  80% of sequence in fragments shorter than    1074 bp
  85% of sequence in fragments shorter than    1221 bp
  90% of sequence in fragments shorter than    1426 bp
  95% of sequence in fragments shorter than    1772 bp

# MboII.SwaI (GAAGA+ATTTAAAT) double digest, 9425 thousand fragments (9199496+226602 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     317 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     416 bp
  40% of sequence in fragments shorter than     469 bp
  45% of sequence in fragments shorter than     524 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     647 bp
  60% of sequence in fragments shorter than     717 bp
  65% of sequence in fragments shorter than     794 bp
  70% of sequence in fragments shorter than     880 bp
  75% of sequence in fragments shorter than     981 bp
  80% of sequence in fragments shorter than    1096 bp
  85% of sequence in fragments shorter than    1250 bp
  90% of sequence in fragments shorter than    1466 bp
  95% of sequence in fragments shorter than    1835 bp

# HaeIII.HindIII (GGCC+AAGCTT) double digest, 9422 thousand fragments (8562230+860365 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     337 bp
  30% of sequence in fragments shorter than     386 bp
  35% of sequence in fragments shorter than     445 bp
  40% of sequence in fragments shorter than     510 bp
  45% of sequence in fragments shorter than     576 bp
  50% of sequence in fragments shorter than     648 bp
  55% of sequence in fragments shorter than     727 bp
  60% of sequence in fragments shorter than     817 bp
  65% of sequence in fragments shorter than     912 bp
  70% of sequence in fragments shorter than    1026 bp
  75% of sequence in fragments shorter than    1159 bp
  80% of sequence in fragments shorter than    1313 bp
  85% of sequence in fragments shorter than    1519 bp
  90% of sequence in fragments shorter than    1811 bp
  95% of sequence in fragments shorter than    2318 bp

# HaeIII.XbaI (GGCC+TCTAGA) double digest, 9412 thousand fragments (8562230+850998 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     333 bp
  30% of sequence in fragments shorter than     384 bp
  35% of sequence in fragments shorter than     442 bp
  40% of sequence in fragments shorter than     507 bp
  45% of sequence in fragments shorter than     573 bp
  50% of sequence in fragments shorter than     647 bp
  55% of sequence in fragments shorter than     725 bp
  60% of sequence in fragments shorter than     813 bp
  65% of sequence in fragments shorter than     906 bp
  70% of sequence in fragments shorter than    1014 bp
  75% of sequence in fragments shorter than    1145 bp
  80% of sequence in fragments shorter than    1306 bp
  85% of sequence in fragments shorter than    1512 bp
  90% of sequence in fragments shorter than    1808 bp
  95% of sequence in fragments shorter than    2324 bp

# ApaLI.HinfI (GTGCAC+GANTC) double digest, 9411 thousand fragments (496255+8916054 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     262 bp
  25% of sequence in fragments shorter than     307 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     384 bp
  40% of sequence in fragments shorter than     432 bp
  45% of sequence in fragments shorter than     481 bp
  50% of sequence in fragments shorter than     531 bp
  55% of sequence in fragments shorter than     585 bp
  60% of sequence in fragments shorter than     646 bp
  65% of sequence in fragments shorter than     709 bp
  70% of sequence in fragments shorter than     784 bp
  75% of sequence in fragments shorter than     868 bp
  80% of sequence in fragments shorter than     972 bp
  85% of sequence in fragments shorter than    1102 bp
  90% of sequence in fragments shorter than    1279 bp
  95% of sequence in fragments shorter than    1583 bp

# EcoRI.HaeIII (GAATTC+GGCC) double digest, 9409 thousand fragments (847401+8562230 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     161 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     280 bp
  25% of sequence in fragments shorter than     335 bp
  30% of sequence in fragments shorter than     388 bp
  35% of sequence in fragments shorter than     447 bp
  40% of sequence in fragments shorter than     512 bp
  45% of sequence in fragments shorter than     579 bp
  50% of sequence in fragments shorter than     652 bp
  55% of sequence in fragments shorter than     732 bp
  60% of sequence in fragments shorter than     822 bp
  65% of sequence in fragments shorter than     916 bp
  70% of sequence in fragments shorter than    1030 bp
  75% of sequence in fragments shorter than    1162 bp
  80% of sequence in fragments shorter than    1318 bp
  85% of sequence in fragments shorter than    1527 bp
  90% of sequence in fragments shorter than    1819 bp
  95% of sequence in fragments shorter than    2332 bp

# AflII.TspRI (CTTAAG+CASTG) double digest, 9408 thousand fragments (643284+8765240 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     351 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     448 bp
  45% of sequence in fragments shorter than     499 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     614 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     751 bp
  70% of sequence in fragments shorter than     834 bp
  75% of sequence in fragments shorter than     933 bp
  80% of sequence in fragments shorter than    1040 bp
  85% of sequence in fragments shorter than    1189 bp
  90% of sequence in fragments shorter than    1391 bp
  95% of sequence in fragments shorter than    1772 bp

# AciI.FokI (CCGC+GGATG) double digest, 9406 thousand fragments (4206130+5201116 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than     103 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     293 bp
  25% of sequence in fragments shorter than     351 bp
  30% of sequence in fragments shorter than     412 bp
  35% of sequence in fragments shorter than     476 bp
  40% of sequence in fragments shorter than     543 bp
  45% of sequence in fragments shorter than     612 bp
  50% of sequence in fragments shorter than     687 bp
  55% of sequence in fragments shorter than     768 bp
  60% of sequence in fragments shorter than     854 bp
  65% of sequence in fragments shorter than     954 bp
  70% of sequence in fragments shorter than    1065 bp
  75% of sequence in fragments shorter than    1197 bp
  80% of sequence in fragments shorter than    1358 bp
  85% of sequence in fragments shorter than    1557 bp
  90% of sequence in fragments shorter than    1845 bp
  95% of sequence in fragments shorter than    2348 bp

# ApaI.Hpy188I (GGGCCC+TCNGA) double digest, 9401 thousand fragments (460339+8942153 sites), 316 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     206 bp
  20% of sequence in fragments shorter than     254 bp
  25% of sequence in fragments shorter than     304 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     396 bp
  40% of sequence in fragments shorter than     448 bp
  45% of sequence in fragments shorter than     502 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     620 bp
  60% of sequence in fragments shorter than     688 bp
  65% of sequence in fragments shorter than     764 bp
  70% of sequence in fragments shorter than     848 bp
  75% of sequence in fragments shorter than     945 bp
  80% of sequence in fragments shorter than    1062 bp
  85% of sequence in fragments shorter than    1208 bp
  90% of sequence in fragments shorter than    1402 bp
  95% of sequence in fragments shorter than    1747 bp

# HaeII.TspRI (RGCGCY+CASTG) double digest, 9394 thousand fragments (630101+8765240 sites), 317 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     263 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     405 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     511 bp
  50% of sequence in fragments shorter than     568 bp
  55% of sequence in fragments shorter than     631 bp
  60% of sequence in fragments shorter than     694 bp
  65% of sequence in fragments shorter than     770 bp
  70% of sequence in fragments shorter than     856 bp
  75% of sequence in fragments shorter than     951 bp
  80% of sequence in fragments shorter than    1067 bp
  85% of sequence in fragments shorter than    1218 bp
  90% of sequence in fragments shorter than    1436 bp
  95% of sequence in fragments shorter than    1794 bp

# BscAI.BsrI (GCATC+ACTGG) double digest, 9393 thousand fragments (3652926+5741310 sites), 317 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     408 bp
  40% of sequence in fragments shorter than     459 bp
  45% of sequence in fragments shorter than     513 bp
  50% of sequence in fragments shorter than     570 bp
  55% of sequence in fragments shorter than     631 bp
  60% of sequence in fragments shorter than     693 bp
  65% of sequence in fragments shorter than     764 bp
  70% of sequence in fragments shorter than     848 bp
  75% of sequence in fragments shorter than     947 bp
  80% of sequence in fragments shorter than    1065 bp
  85% of sequence in fragments shorter than    1219 bp
  90% of sequence in fragments shorter than    1423 bp
  95% of sequence in fragments shorter than    1797 bp

# EcoRV.Hpy188I (GATATC+TCNGA) double digest, 9388 thousand fragments (446473+8942153 sites), 317 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     562 bp
  55% of sequence in fragments shorter than     622 bp
  60% of sequence in fragments shorter than     689 bp
  65% of sequence in fragments shorter than     764 bp
  70% of sequence in fragments shorter than     847 bp
  75% of sequence in fragments shorter than     943 bp
  80% of sequence in fragments shorter than    1058 bp
  85% of sequence in fragments shorter than    1201 bp
  90% of sequence in fragments shorter than    1392 bp
  95% of sequence in fragments shorter than    1731 bp

# AvaII.BsmAI (GGWCC+GTCTC) double digest, 9378 thousand fragments (2747690+6631585 sites), 317 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     201 bp
  20% of sequence in fragments shorter than     255 bp
  25% of sequence in fragments shorter than     309 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     414 bp
  40% of sequence in fragments shorter than     473 bp
  45% of sequence in fragments shorter than     534 bp
  50% of sequence in fragments shorter than     598 bp
  55% of sequence in fragments shorter than     671 bp
  60% of sequence in fragments shorter than     750 bp
  65% of sequence in fragments shorter than     842 bp
  70% of sequence in fragments shorter than     949 bp
  75% of sequence in fragments shorter than    1076 bp
  80% of sequence in fragments shorter than    1231 bp
  85% of sequence in fragments shorter than    1430 bp
  90% of sequence in fragments shorter than    1726 bp
  95% of sequence in fragments shorter than    2239 bp

# Hin4II.HpaII (CCTTC+CCGG) double digest, 9377 thousand fragments (7059915+2317722 sites), 317 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     381 bp
  35% of sequence in fragments shorter than     437 bp
  40% of sequence in fragments shorter than     497 bp
  45% of sequence in fragments shorter than     555 bp
  50% of sequence in fragments shorter than     623 bp
  55% of sequence in fragments shorter than     693 bp
  60% of sequence in fragments shorter than     774 bp
  65% of sequence in fragments shorter than     862 bp
  70% of sequence in fragments shorter than     964 bp
  75% of sequence in fragments shorter than    1079 bp
  80% of sequence in fragments shorter than    1223 bp
  85% of sequence in fragments shorter than    1406 bp
  90% of sequence in fragments shorter than    1665 bp
  95% of sequence in fragments shorter than    2102 bp

# ApaI.HinfI (GGGCCC+GANTC) double digest, 9375 thousand fragments (460339+8916054 sites), 317 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     262 bp
  25% of sequence in fragments shorter than     308 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     434 bp
  45% of sequence in fragments shorter than     484 bp
  50% of sequence in fragments shorter than     536 bp
  55% of sequence in fragments shorter than     594 bp
  60% of sequence in fragments shorter than     656 bp
  65% of sequence in fragments shorter than     719 bp
  70% of sequence in fragments shorter than     795 bp
  75% of sequence in fragments shorter than     883 bp
  80% of sequence in fragments shorter than     989 bp
  85% of sequence in fragments shorter than    1122 bp
  90% of sequence in fragments shorter than    1304 bp
  95% of sequence in fragments shorter than    1620 bp

# AclI.MboII (AACGTT+GAAGA) double digest, 9371 thousand fragments (172706+9199496 sites), 317 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     367 bp
  35% of sequence in fragments shorter than     419 bp
  40% of sequence in fragments shorter than     473 bp
  45% of sequence in fragments shorter than     529 bp
  50% of sequence in fragments shorter than     588 bp
  55% of sequence in fragments shorter than     653 bp
  60% of sequence in fragments shorter than     724 bp
  65% of sequence in fragments shorter than     802 bp
  70% of sequence in fragments shorter than     888 bp
  75% of sequence in fragments shorter than     990 bp
  80% of sequence in fragments shorter than    1106 bp
  85% of sequence in fragments shorter than    1262 bp
  90% of sequence in fragments shorter than    1478 bp
  95% of sequence in fragments shorter than    1850 bp

# SacI.TspRI (GAGCTC+CASTG) double digest, 9368 thousand fragments (603323+8765240 sites), 318 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     262 bp
  25% of sequence in fragments shorter than     309 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     405 bp
  40% of sequence in fragments shorter than     455 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     566 bp
  55% of sequence in fragments shorter than     627 bp
  60% of sequence in fragments shorter than     695 bp
  65% of sequence in fragments shorter than     771 bp
  70% of sequence in fragments shorter than     858 bp
  75% of sequence in fragments shorter than     955 bp
  80% of sequence in fragments shorter than    1076 bp
  85% of sequence in fragments shorter than    1231 bp
  90% of sequence in fragments shorter than    1454 bp
  95% of sequence in fragments shorter than    1854 bp

# EcoRV.HinfI (GATATC+GANTC) double digest, 9362 thousand fragments (446473+8916054 sites), 318 bp average fragment length
   5% of sequence in fragments shorter than     107 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     263 bp
  25% of sequence in fragments shorter than     308 bp
  30% of sequence in fragments shorter than     345 bp
  35% of sequence in fragments shorter than     386 bp
  40% of sequence in fragments shorter than     434 bp
  45% of sequence in fragments shorter than     483 bp
  50% of sequence in fragments shorter than     535 bp
  55% of sequence in fragments shorter than     592 bp
  60% of sequence in fragments shorter than     652 bp
  65% of sequence in fragments shorter than     715 bp
  70% of sequence in fragments shorter than     789 bp
  75% of sequence in fragments shorter than     874 bp
  80% of sequence in fragments shorter than     977 bp
  85% of sequence in fragments shorter than    1106 bp
  90% of sequence in fragments shorter than    1282 bp
  95% of sequence in fragments shorter than    1582 bp

# AvrII.TspRI (CCTAGG+CASTG) double digest, 9359 thousand fragments (594956+8765240 sites), 318 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     507 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     688 bp
  65% of sequence in fragments shorter than     763 bp
  70% of sequence in fragments shorter than     849 bp
  75% of sequence in fragments shorter than     950 bp
  80% of sequence in fragments shorter than    1070 bp
  85% of sequence in fragments shorter than    1226 bp
  90% of sequence in fragments shorter than    1442 bp
  95% of sequence in fragments shorter than    1837 bp

# BsmAI.SmlI (GTCTC+CTYRAG) double digest, 9358 thousand fragments (6631585+2727867 sites), 318 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     376 bp
  35% of sequence in fragments shorter than     432 bp
  40% of sequence in fragments shorter than     490 bp
  45% of sequence in fragments shorter than     548 bp
  50% of sequence in fragments shorter than     609 bp
  55% of sequence in fragments shorter than     677 bp
  60% of sequence in fragments shorter than     753 bp
  65% of sequence in fragments shorter than     840 bp
  70% of sequence in fragments shorter than     936 bp
  75% of sequence in fragments shorter than    1050 bp
  80% of sequence in fragments shorter than    1192 bp
  85% of sequence in fragments shorter than    1374 bp
  90% of sequence in fragments shorter than    1641 bp
  95% of sequence in fragments shorter than    2107 bp

# MboII.PacI (GAAGA+TTAATTAA) double digest, 9358 thousand fragments (9199496+159455 sites), 318 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     367 bp
  35% of sequence in fragments shorter than     420 bp
  40% of sequence in fragments shorter than     473 bp
  45% of sequence in fragments shorter than     528 bp
  50% of sequence in fragments shorter than     588 bp
  55% of sequence in fragments shorter than     652 bp
  60% of sequence in fragments shorter than     723 bp
  65% of sequence in fragments shorter than     801 bp
  70% of sequence in fragments shorter than     887 bp
  75% of sequence in fragments shorter than     990 bp
  80% of sequence in fragments shorter than    1106 bp
  85% of sequence in fragments shorter than    1261 bp
  90% of sequence in fragments shorter than    1478 bp
  95% of sequence in fragments shorter than    1849 bp

# Hpy99I.MboII (CGWCG+GAAGA) double digest, 9356 thousand fragments (157917+9199496 sites), 318 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     367 bp
  35% of sequence in fragments shorter than     420 bp
  40% of sequence in fragments shorter than     473 bp
  45% of sequence in fragments shorter than     528 bp
  50% of sequence in fragments shorter than     588 bp
  55% of sequence in fragments shorter than     652 bp
  60% of sequence in fragments shorter than     723 bp
  65% of sequence in fragments shorter than     801 bp
  70% of sequence in fragments shorter than     887 bp
  75% of sequence in fragments shorter than     989 bp
  80% of sequence in fragments shorter than    1104 bp
  85% of sequence in fragments shorter than    1258 bp
  90% of sequence in fragments shorter than    1473 bp
  95% of sequence in fragments shorter than    1839 bp

# Hpy188I.SpeI (TCNGA+ACTAGT) double digest, 9341 thousand fragments (8942153+400286 sites), 318 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     398 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     561 bp
  55% of sequence in fragments shorter than     621 bp
  60% of sequence in fragments shorter than     687 bp
  65% of sequence in fragments shorter than     761 bp
  70% of sequence in fragments shorter than     844 bp
  75% of sequence in fragments shorter than     941 bp
  80% of sequence in fragments shorter than    1056 bp
  85% of sequence in fragments shorter than    1200 bp
  90% of sequence in fragments shorter than    1391 bp
  95% of sequence in fragments shorter than    1733 bp

# HgaI.TspRI (GACGC+CASTG) double digest, 9336 thousand fragments (571889+8765240 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     458 bp
  45% of sequence in fragments shorter than     513 bp
  50% of sequence in fragments shorter than     570 bp
  55% of sequence in fragments shorter than     633 bp
  60% of sequence in fragments shorter than     700 bp
  65% of sequence in fragments shorter than     776 bp
  70% of sequence in fragments shorter than     863 bp
  75% of sequence in fragments shorter than     962 bp
  80% of sequence in fragments shorter than    1082 bp
  85% of sequence in fragments shorter than    1240 bp
  90% of sequence in fragments shorter than    1458 bp
  95% of sequence in fragments shorter than    1859 bp

# BglII.HaeIII (AGATCT+GGCC) double digest, 9336 thousand fragments (774732+8562230 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     162 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     282 bp
  25% of sequence in fragments shorter than     340 bp
  30% of sequence in fragments shorter than     390 bp
  35% of sequence in fragments shorter than     451 bp
  40% of sequence in fragments shorter than     516 bp
  45% of sequence in fragments shorter than     584 bp
  50% of sequence in fragments shorter than     656 bp
  55% of sequence in fragments shorter than     735 bp
  60% of sequence in fragments shorter than     826 bp
  65% of sequence in fragments shorter than     920 bp
  70% of sequence in fragments shorter than    1036 bp
  75% of sequence in fragments shorter than    1171 bp
  80% of sequence in fragments shorter than    1331 bp
  85% of sequence in fragments shorter than    1543 bp
  90% of sequence in fragments shorter than    1848 bp
  95% of sequence in fragments shorter than    2372 bp

# EciI.TspRI (GGCGGA+CASTG) double digest, 9336 thousand fragments (571273+8765240 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     116 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     314 bp
  30% of sequence in fragments shorter than     362 bp
  35% of sequence in fragments shorter than     413 bp
  40% of sequence in fragments shorter than     466 bp
  45% of sequence in fragments shorter than     521 bp
  50% of sequence in fragments shorter than     580 bp
  55% of sequence in fragments shorter than     644 bp
  60% of sequence in fragments shorter than     713 bp
  65% of sequence in fragments shorter than     792 bp
  70% of sequence in fragments shorter than     882 bp
  75% of sequence in fragments shorter than     982 bp
  80% of sequence in fragments shorter than    1106 bp
  85% of sequence in fragments shorter than    1270 bp
  90% of sequence in fragments shorter than    1496 bp
  95% of sequence in fragments shorter than    1905 bp

# MboII.SnaBI (GAAGA+TACGTA) double digest, 9331 thousand fragments (9199496+132299 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     368 bp
  35% of sequence in fragments shorter than     421 bp
  40% of sequence in fragments shorter than     474 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     654 bp
  60% of sequence in fragments shorter than     725 bp
  65% of sequence in fragments shorter than     803 bp
  70% of sequence in fragments shorter than     890 bp
  75% of sequence in fragments shorter than     992 bp
  80% of sequence in fragments shorter than    1109 bp
  85% of sequence in fragments shorter than    1265 bp
  90% of sequence in fragments shorter than    1483 bp
  95% of sequence in fragments shorter than    1855 bp

# HpaI.Hpy188I (GTTAAC+TCNGA) double digest, 9331 thousand fragments (389418+8942153 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     208 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     349 bp
  35% of sequence in fragments shorter than     398 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     561 bp
  55% of sequence in fragments shorter than     621 bp
  60% of sequence in fragments shorter than     688 bp
  65% of sequence in fragments shorter than     763 bp
  70% of sequence in fragments shorter than     845 bp
  75% of sequence in fragments shorter than     942 bp
  80% of sequence in fragments shorter than    1057 bp
  85% of sequence in fragments shorter than    1201 bp
  90% of sequence in fragments shorter than    1392 bp
  95% of sequence in fragments shorter than    1731 bp

# MfeI.TspRI (CAATTG+CASTG) double digest, 9329 thousand fragments (564303+8765240 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     309 bp
  30% of sequence in fragments shorter than     354 bp
  35% of sequence in fragments shorter than     403 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     619 bp
  60% of sequence in fragments shorter than     683 bp
  65% of sequence in fragments shorter than     756 bp
  70% of sequence in fragments shorter than     839 bp
  75% of sequence in fragments shorter than     936 bp
  80% of sequence in fragments shorter than    1046 bp
  85% of sequence in fragments shorter than    1193 bp
  90% of sequence in fragments shorter than    1402 bp
  95% of sequence in fragments shorter than    1760 bp

# MboII.NaeI (GAAGA+GCCGGC) double digest, 9324 thousand fragments (9199496+125692 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     270 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     368 bp
  35% of sequence in fragments shorter than     421 bp
  40% of sequence in fragments shorter than     474 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     589 bp
  55% of sequence in fragments shorter than     654 bp
  60% of sequence in fragments shorter than     725 bp
  65% of sequence in fragments shorter than     802 bp
  70% of sequence in fragments shorter than     889 bp
  75% of sequence in fragments shorter than     990 bp
  80% of sequence in fragments shorter than    1105 bp
  85% of sequence in fragments shorter than    1259 bp
  90% of sequence in fragments shorter than    1474 bp
  95% of sequence in fragments shorter than    1840 bp

# BthCI.StyI (GCNGC+CCWWGG) double digest, 9323 thousand fragments (6209929+3114107 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     104 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     232 bp
  20% of sequence in fragments shorter than     291 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     411 bp
  35% of sequence in fragments shorter than     475 bp
  40% of sequence in fragments shorter than     544 bp
  45% of sequence in fragments shorter than     618 bp
  50% of sequence in fragments shorter than     691 bp
  55% of sequence in fragments shorter than     777 bp
  60% of sequence in fragments shorter than     868 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1094 bp
  75% of sequence in fragments shorter than    1237 bp
  80% of sequence in fragments shorter than    1406 bp
  85% of sequence in fragments shorter than    1635 bp
  90% of sequence in fragments shorter than    1951 bp
  95% of sequence in fragments shorter than    2485 bp

# HaeIII.NcoI (GGCC+CCATGG) double digest, 9321 thousand fragments (8562230+759594 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     287 bp
  25% of sequence in fragments shorter than     345 bp
  30% of sequence in fragments shorter than     400 bp
  35% of sequence in fragments shorter than     462 bp
  40% of sequence in fragments shorter than     528 bp
  45% of sequence in fragments shorter than     603 bp
  50% of sequence in fragments shorter than     684 bp
  55% of sequence in fragments shorter than     769 bp
  60% of sequence in fragments shorter than     863 bp
  65% of sequence in fragments shorter than     970 bp
  70% of sequence in fragments shorter than    1092 bp
  75% of sequence in fragments shorter than    1243 bp
  80% of sequence in fragments shorter than    1414 bp
  85% of sequence in fragments shorter than    1651 bp
  90% of sequence in fragments shorter than    1977 bp
  95% of sequence in fragments shorter than    2562 bp

# AciI.CviQI (CCGC+GTAC) double digest, 9320 thousand fragments (4206130+5115078 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     230 bp
  20% of sequence in fragments shorter than     286 bp
  25% of sequence in fragments shorter than     340 bp
  30% of sequence in fragments shorter than     394 bp
  35% of sequence in fragments shorter than     449 bp
  40% of sequence in fragments shorter than     505 bp
  45% of sequence in fragments shorter than     564 bp
  50% of sequence in fragments shorter than     627 bp
  55% of sequence in fragments shorter than     694 bp
  60% of sequence in fragments shorter than     767 bp
  65% of sequence in fragments shorter than     847 bp
  70% of sequence in fragments shorter than     938 bp
  75% of sequence in fragments shorter than    1042 bp
  80% of sequence in fragments shorter than    1170 bp
  85% of sequence in fragments shorter than    1326 bp
  90% of sequence in fragments shorter than    1545 bp
  95% of sequence in fragments shorter than    1929 bp

# MboII.XhoI (GAAGA+CTCGAG) double digest, 9320 thousand fragments (9199496+121308 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     270 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     368 bp
  35% of sequence in fragments shorter than     422 bp
  40% of sequence in fragments shorter than     475 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     655 bp
  60% of sequence in fragments shorter than     726 bp
  65% of sequence in fragments shorter than     804 bp
  70% of sequence in fragments shorter than     890 bp
  75% of sequence in fragments shorter than     992 bp
  80% of sequence in fragments shorter than    1108 bp
  85% of sequence in fragments shorter than    1263 bp
  90% of sequence in fragments shorter than    1479 bp
  95% of sequence in fragments shorter than    1847 bp

# Hpy188I.SapI (TCNGA+GCTCTTC) double digest, 9318 thousand fragments (8942153+377161 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     257 bp
  25% of sequence in fragments shorter than     307 bp
  30% of sequence in fragments shorter than     351 bp
  35% of sequence in fragments shorter than     400 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     507 bp
  50% of sequence in fragments shorter than     565 bp
  55% of sequence in fragments shorter than     626 bp
  60% of sequence in fragments shorter than     694 bp
  65% of sequence in fragments shorter than     770 bp
  70% of sequence in fragments shorter than     854 bp
  75% of sequence in fragments shorter than     952 bp
  80% of sequence in fragments shorter than    1069 bp
  85% of sequence in fragments shorter than    1215 bp
  90% of sequence in fragments shorter than    1411 bp
  95% of sequence in fragments shorter than    1756 bp

# Hpy188I.SmaI (TCNGA+CCCGGG) double digest, 9318 thousand fragments (8942153+376940 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     305 bp
  30% of sequence in fragments shorter than     348 bp
  35% of sequence in fragments shorter than     396 bp
  40% of sequence in fragments shorter than     446 bp
  45% of sequence in fragments shorter than     498 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     612 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     752 bp
  70% of sequence in fragments shorter than     832 bp
  75% of sequence in fragments shorter than     927 bp
  80% of sequence in fragments shorter than    1038 bp
  85% of sequence in fragments shorter than    1180 bp
  90% of sequence in fragments shorter than    1362 bp
  95% of sequence in fragments shorter than    1688 bp

# HinfI.SpeI (GANTC+ACTAGT) double digest, 9315 thousand fragments (8916054+400286 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     308 bp
  30% of sequence in fragments shorter than     346 bp
  35% of sequence in fragments shorter than     388 bp
  40% of sequence in fragments shorter than     437 bp
  45% of sequence in fragments shorter than     486 bp
  50% of sequence in fragments shorter than     538 bp
  55% of sequence in fragments shorter than     595 bp
  60% of sequence in fragments shorter than     656 bp
  65% of sequence in fragments shorter than     719 bp
  70% of sequence in fragments shorter than     793 bp
  75% of sequence in fragments shorter than     879 bp
  80% of sequence in fragments shorter than     984 bp
  85% of sequence in fragments shorter than    1114 bp
  90% of sequence in fragments shorter than    1292 bp
  95% of sequence in fragments shorter than    1598 bp

# SphI.TspRI (GCATGC+CASTG) double digest, 9315 thousand fragments (550952+8765240 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     312 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     408 bp
  40% of sequence in fragments shorter than     459 bp
  45% of sequence in fragments shorter than     513 bp
  50% of sequence in fragments shorter than     570 bp
  55% of sequence in fragments shorter than     632 bp
  60% of sequence in fragments shorter than     698 bp
  65% of sequence in fragments shorter than     775 bp
  70% of sequence in fragments shorter than     861 bp
  75% of sequence in fragments shorter than     958 bp
  80% of sequence in fragments shorter than    1079 bp
  85% of sequence in fragments shorter than    1237 bp
  90% of sequence in fragments shorter than    1456 bp
  95% of sequence in fragments shorter than    1854 bp

# BthCI.NspI (GCNGC+RCATGY) double digest, 9313 thousand fragments (6209929+3104359 sites), 319 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     235 bp
  20% of sequence in fragments shorter than     293 bp
  25% of sequence in fragments shorter than     346 bp
  30% of sequence in fragments shorter than     405 bp
  35% of sequence in fragments shorter than     466 bp
  40% of sequence in fragments shorter than     530 bp
  45% of sequence in fragments shorter than     595 bp
  50% of sequence in fragments shorter than     666 bp
  55% of sequence in fragments shorter than     743 bp
  60% of sequence in fragments shorter than     826 bp
  65% of sequence in fragments shorter than     915 bp
  70% of sequence in fragments shorter than    1022 bp
  75% of sequence in fragments shorter than    1142 bp
  80% of sequence in fragments shorter than    1293 bp
  85% of sequence in fragments shorter than    1485 bp
  90% of sequence in fragments shorter than    1744 bp
  95% of sequence in fragments shorter than    2191 bp

# BbvI.Tsp45I (GCAGC+GTSAC) double digest, 9311 thousand fragments (5290051+4021761 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     325 bp
  30% of sequence in fragments shorter than     377 bp
  35% of sequence in fragments shorter than     432 bp
  40% of sequence in fragments shorter than     492 bp
  45% of sequence in fragments shorter than     555 bp
  50% of sequence in fragments shorter than     621 bp
  55% of sequence in fragments shorter than     693 bp
  60% of sequence in fragments shorter than     774 bp
  65% of sequence in fragments shorter than     862 bp
  70% of sequence in fragments shorter than     965 bp
  75% of sequence in fragments shorter than    1085 bp
  80% of sequence in fragments shorter than    1236 bp
  85% of sequence in fragments shorter than    1422 bp
  90% of sequence in fragments shorter than    1680 bp
  95% of sequence in fragments shorter than    2135 bp

# ScaI.TspRI (AGTACT+CASTG) double digest, 9308 thousand fragments (543793+8765240 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     506 bp
  50% of sequence in fragments shorter than     561 bp
  55% of sequence in fragments shorter than     622 bp
  60% of sequence in fragments shorter than     687 bp
  65% of sequence in fragments shorter than     761 bp
  70% of sequence in fragments shorter than     845 bp
  75% of sequence in fragments shorter than     943 bp
  80% of sequence in fragments shorter than    1057 bp
  85% of sequence in fragments shorter than    1207 bp
  90% of sequence in fragments shorter than    1418 bp
  95% of sequence in fragments shorter than    1801 bp

# BamHI.Hpy188I (GGATCC+TCNGA) double digest, 9307 thousand fragments (365999+8942153 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     209 bp
  20% of sequence in fragments shorter than     256 bp
  25% of sequence in fragments shorter than     306 bp
  30% of sequence in fragments shorter than     351 bp
  35% of sequence in fragments shorter than     401 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     507 bp
  50% of sequence in fragments shorter than     565 bp
  55% of sequence in fragments shorter than     626 bp
  60% of sequence in fragments shorter than     694 bp
  65% of sequence in fragments shorter than     769 bp
  70% of sequence in fragments shorter than     853 bp
  75% of sequence in fragments shorter than     950 bp
  80% of sequence in fragments shorter than    1067 bp
  85% of sequence in fragments shorter than    1212 bp
  90% of sequence in fragments shorter than    1406 bp
  95% of sequence in fragments shorter than    1749 bp

# HinfI.HpaI (GANTC+GTTAAC) double digest, 9304 thousand fragments (8916054+389418 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     346 bp
  35% of sequence in fragments shorter than     387 bp
  40% of sequence in fragments shorter than     435 bp
  45% of sequence in fragments shorter than     485 bp
  50% of sequence in fragments shorter than     537 bp
  55% of sequence in fragments shorter than     594 bp
  60% of sequence in fragments shorter than     655 bp
  65% of sequence in fragments shorter than     717 bp
  70% of sequence in fragments shorter than     792 bp
  75% of sequence in fragments shorter than     878 bp
  80% of sequence in fragments shorter than     983 bp
  85% of sequence in fragments shorter than    1113 bp
  90% of sequence in fragments shorter than    1290 bp
  95% of sequence in fragments shorter than    1593 bp

# BclI.HaeIII (TGATCA+GGCC) double digest, 9300 thousand fragments (738785+8562230 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     282 bp
  25% of sequence in fragments shorter than     342 bp
  30% of sequence in fragments shorter than     392 bp
  35% of sequence in fragments shorter than     452 bp
  40% of sequence in fragments shorter than     517 bp
  45% of sequence in fragments shorter than     585 bp
  50% of sequence in fragments shorter than     659 bp
  55% of sequence in fragments shorter than     739 bp
  60% of sequence in fragments shorter than     831 bp
  65% of sequence in fragments shorter than     926 bp
  70% of sequence in fragments shorter than    1040 bp
  75% of sequence in fragments shorter than    1177 bp
  80% of sequence in fragments shorter than    1335 bp
  85% of sequence in fragments shorter than    1545 bp
  90% of sequence in fragments shorter than    1842 bp
  95% of sequence in fragments shorter than    2365 bp

# FnuDII.HaeIII (CGCG+GGCC) double digest, 9295 thousand fragments (733940+8562230 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     294 bp
  25% of sequence in fragments shorter than     349 bp
  30% of sequence in fragments shorter than     418 bp
  35% of sequence in fragments shorter than     487 bp
  40% of sequence in fragments shorter than     558 bp
  45% of sequence in fragments shorter than     638 bp
  50% of sequence in fragments shorter than     723 bp
  55% of sequence in fragments shorter than     820 bp
  60% of sequence in fragments shorter than     919 bp
  65% of sequence in fragments shorter than    1037 bp
  70% of sequence in fragments shorter than    1174 bp
  75% of sequence in fragments shorter than    1328 bp
  80% of sequence in fragments shorter than    1526 bp
  85% of sequence in fragments shorter than    1779 bp
  90% of sequence in fragments shorter than    2142 bp
  95% of sequence in fragments shorter than    2779 bp

# HinfI.SapI (GANTC+GCTCTTC) double digest, 9292 thousand fragments (8916054+377161 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     390 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     489 bp
  50% of sequence in fragments shorter than     541 bp
  55% of sequence in fragments shorter than     598 bp
  60% of sequence in fragments shorter than     660 bp
  65% of sequence in fragments shorter than     724 bp
  70% of sequence in fragments shorter than     799 bp
  75% of sequence in fragments shorter than     887 bp
  80% of sequence in fragments shorter than     993 bp
  85% of sequence in fragments shorter than    1125 bp
  90% of sequence in fragments shorter than    1305 bp
  95% of sequence in fragments shorter than    1617 bp

# HinfI.SmaI (GANTC+CCCGGG) double digest, 9292 thousand fragments (8916054+376940 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     313 bp
  30% of sequence in fragments shorter than     348 bp
  35% of sequence in fragments shorter than     393 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     493 bp
  50% of sequence in fragments shorter than     546 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     667 bp
  65% of sequence in fragments shorter than     732 bp
  70% of sequence in fragments shorter than     808 bp
  75% of sequence in fragments shorter than     898 bp
  80% of sequence in fragments shorter than    1006 bp
  85% of sequence in fragments shorter than    1139 bp
  90% of sequence in fragments shorter than    1324 bp
  95% of sequence in fragments shorter than    1640 bp

# ClaI.MboII (ATCGAT+GAAGA) double digest, 9285 thousand fragments (86477+9199496 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     370 bp
  35% of sequence in fragments shorter than     424 bp
  40% of sequence in fragments shorter than     477 bp
  45% of sequence in fragments shorter than     533 bp
  50% of sequence in fragments shorter than     593 bp
  55% of sequence in fragments shorter than     658 bp
  60% of sequence in fragments shorter than     730 bp
  65% of sequence in fragments shorter than     808 bp
  70% of sequence in fragments shorter than     895 bp
  75% of sequence in fragments shorter than     998 bp
  80% of sequence in fragments shorter than    1117 bp
  85% of sequence in fragments shorter than    1274 bp
  90% of sequence in fragments shorter than    1493 bp
  95% of sequence in fragments shorter than    1868 bp

# BccI.StyI (CCATC+CCWWGG) double digest, 9284 thousand fragments (6170927+3114107 sites), 320 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     313 bp
  30% of sequence in fragments shorter than     361 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     461 bp
  45% of sequence in fragments shorter than     518 bp
  50% of sequence in fragments shorter than     578 bp
  55% of sequence in fragments shorter than     644 bp
  60% of sequence in fragments shorter than     715 bp
  65% of sequence in fragments shorter than     796 bp
  70% of sequence in fragments shorter than     889 bp
  75% of sequence in fragments shorter than     999 bp
  80% of sequence in fragments shorter than    1128 bp
  85% of sequence in fragments shorter than    1300 bp
  90% of sequence in fragments shorter than    1548 bp
  95% of sequence in fragments shorter than    2017 bp

# BamHI.HinfI (GGATCC+GANTC) double digest, 9281 thousand fragments (365999+8916054 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     346 bp
  35% of sequence in fragments shorter than     390 bp
  40% of sequence in fragments shorter than     439 bp
  45% of sequence in fragments shorter than     489 bp
  50% of sequence in fragments shorter than     541 bp
  55% of sequence in fragments shorter than     599 bp
  60% of sequence in fragments shorter than     661 bp
  65% of sequence in fragments shorter than     725 bp
  70% of sequence in fragments shorter than     801 bp
  75% of sequence in fragments shorter than     889 bp
  80% of sequence in fragments shorter than     996 bp
  85% of sequence in fragments shorter than    1128 bp
  90% of sequence in fragments shorter than    1309 bp
  95% of sequence in fragments shorter than    1623 bp

# BstEII.Hpy188I (GGTNACC+TCNGA) double digest, 9277 thousand fragments (335865+8942153 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     258 bp
  25% of sequence in fragments shorter than     308 bp
  30% of sequence in fragments shorter than     351 bp
  35% of sequence in fragments shorter than     402 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     508 bp
  50% of sequence in fragments shorter than     566 bp
  55% of sequence in fragments shorter than     626 bp
  60% of sequence in fragments shorter than     694 bp
  65% of sequence in fragments shorter than     770 bp
  70% of sequence in fragments shorter than     854 bp
  75% of sequence in fragments shorter than     951 bp
  80% of sequence in fragments shorter than    1068 bp
  85% of sequence in fragments shorter than    1213 bp
  90% of sequence in fragments shorter than    1407 bp
  95% of sequence in fragments shorter than    1751 bp

# BsaAI.HaeIII (YACGTR+GGCC) double digest, 9275 thousand fragments (713801+8562230 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     164 bp
  15% of sequence in fragments shorter than     224 bp
  20% of sequence in fragments shorter than     285 bp
  25% of sequence in fragments shorter than     343 bp
  30% of sequence in fragments shorter than     395 bp
  35% of sequence in fragments shorter than     458 bp
  40% of sequence in fragments shorter than     522 bp
  45% of sequence in fragments shorter than     594 bp
  50% of sequence in fragments shorter than     673 bp
  55% of sequence in fragments shorter than     754 bp
  60% of sequence in fragments shorter than     850 bp
  65% of sequence in fragments shorter than     949 bp
  70% of sequence in fragments shorter than    1071 bp
  75% of sequence in fragments shorter than    1215 bp
  80% of sequence in fragments shorter than    1383 bp
  85% of sequence in fragments shorter than    1608 bp
  90% of sequence in fragments shorter than    1924 bp
  95% of sequence in fragments shorter than    2481 bp

# BccI.NspI (CCATC+RCATGY) double digest, 9274 thousand fragments (6170927+3104359 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     314 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     457 bp
  45% of sequence in fragments shorter than     510 bp
  50% of sequence in fragments shorter than     566 bp
  55% of sequence in fragments shorter than     626 bp
  60% of sequence in fragments shorter than     692 bp
  65% of sequence in fragments shorter than     765 bp
  70% of sequence in fragments shorter than     848 bp
  75% of sequence in fragments shorter than     947 bp
  80% of sequence in fragments shorter than    1065 bp
  85% of sequence in fragments shorter than    1212 bp
  90% of sequence in fragments shorter than    1431 bp
  95% of sequence in fragments shorter than    1833 bp

# BsrI.SfeI (ACTGG+CTRYAG) double digest, 9271 thousand fragments (5741310+3531009 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     307 bp
  30% of sequence in fragments shorter than     352 bp
  35% of sequence in fragments shorter than     401 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     499 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     613 bp
  60% of sequence in fragments shorter than     675 bp
  65% of sequence in fragments shorter than     740 bp
  70% of sequence in fragments shorter than     821 bp
  75% of sequence in fragments shorter than     912 bp
  80% of sequence in fragments shorter than    1024 bp
  85% of sequence in fragments shorter than    1163 bp
  90% of sequence in fragments shorter than    1363 bp
  95% of sequence in fragments shorter than    1686 bp

# AatII.MboII (GACGTC+GAAGA) double digest, 9270 thousand fragments (71089+9199496 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     371 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     478 bp
  45% of sequence in fragments shorter than     534 bp
  50% of sequence in fragments shorter than     595 bp
  55% of sequence in fragments shorter than     660 bp
  60% of sequence in fragments shorter than     732 bp
  65% of sequence in fragments shorter than     810 bp
  70% of sequence in fragments shorter than     898 bp
  75% of sequence in fragments shorter than    1001 bp
  80% of sequence in fragments shorter than    1118 bp
  85% of sequence in fragments shorter than    1275 bp
  90% of sequence in fragments shorter than    1494 bp
  95% of sequence in fragments shorter than    1868 bp

# BceAI.HaeIII (ACGGC+GGCC) double digest, 9266 thousand fragments (705178+8562230 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     293 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     412 bp
  35% of sequence in fragments shorter than     478 bp
  40% of sequence in fragments shorter than     547 bp
  45% of sequence in fragments shorter than     624 bp
  50% of sequence in fragments shorter than     706 bp
  55% of sequence in fragments shorter than     799 bp
  60% of sequence in fragments shorter than     894 bp
  65% of sequence in fragments shorter than    1010 bp
  70% of sequence in fragments shorter than    1141 bp
  75% of sequence in fragments shorter than    1289 bp
  80% of sequence in fragments shorter than    1478 bp
  85% of sequence in fragments shorter than    1723 bp
  90% of sequence in fragments shorter than    2069 bp
  95% of sequence in fragments shorter than    2681 bp

# MboII.SacII (GAAGA+CCGCGG) double digest, 9265 thousand fragments (9199496+66901 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     371 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     478 bp
  45% of sequence in fragments shorter than     534 bp
  50% of sequence in fragments shorter than     595 bp
  55% of sequence in fragments shorter than     660 bp
  60% of sequence in fragments shorter than     731 bp
  65% of sequence in fragments shorter than     810 bp
  70% of sequence in fragments shorter than     897 bp
  75% of sequence in fragments shorter than    1000 bp
  80% of sequence in fragments shorter than    1117 bp
  85% of sequence in fragments shorter than    1274 bp
  90% of sequence in fragments shorter than    1492 bp
  95% of sequence in fragments shorter than    1865 bp

# ApaLI.TspRI (GTGCAC+CASTG) double digest, 9260 thousand fragments (496255+8765240 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     364 bp
  35% of sequence in fragments shorter than     413 bp
  40% of sequence in fragments shorter than     465 bp
  45% of sequence in fragments shorter than     520 bp
  50% of sequence in fragments shorter than     576 bp
  55% of sequence in fragments shorter than     639 bp
  60% of sequence in fragments shorter than     706 bp
  65% of sequence in fragments shorter than     784 bp
  70% of sequence in fragments shorter than     872 bp
  75% of sequence in fragments shorter than     971 bp
  80% of sequence in fragments shorter than    1093 bp
  85% of sequence in fragments shorter than    1254 bp
  90% of sequence in fragments shorter than    1477 bp
  95% of sequence in fragments shorter than    1880 bp

# MboII.PspXI (GAAGA+VCTCGAGB) double digest, 9258 thousand fragments (9199496+59193 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     272 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     371 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     479 bp
  45% of sequence in fragments shorter than     535 bp
  50% of sequence in fragments shorter than     595 bp
  55% of sequence in fragments shorter than     660 bp
  60% of sequence in fragments shorter than     732 bp
  65% of sequence in fragments shorter than     811 bp
  70% of sequence in fragments shorter than     898 bp
  75% of sequence in fragments shorter than    1001 bp
  80% of sequence in fragments shorter than    1118 bp
  85% of sequence in fragments shorter than    1275 bp
  90% of sequence in fragments shorter than    1494 bp
  95% of sequence in fragments shorter than    1867 bp

# BciVI.HaeIII (GTATCC+GGCC) double digest, 9257 thousand fragments (696093+8562230 sites), 321 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     282 bp
  25% of sequence in fragments shorter than     342 bp
  30% of sequence in fragments shorter than     392 bp
  35% of sequence in fragments shorter than     456 bp
  40% of sequence in fragments shorter than     520 bp
  45% of sequence in fragments shorter than     589 bp
  50% of sequence in fragments shorter than     666 bp
  55% of sequence in fragments shorter than     746 bp
  60% of sequence in fragments shorter than     838 bp
  65% of sequence in fragments shorter than     935 bp
  70% of sequence in fragments shorter than    1050 bp
  75% of sequence in fragments shorter than    1186 bp
  80% of sequence in fragments shorter than    1351 bp
  85% of sequence in fragments shorter than    1566 bp
  90% of sequence in fragments shorter than    1867 bp
  95% of sequence in fragments shorter than    2395 bp

# AgeI.MboII (ACCGGT+GAAGA) double digest, 9252 thousand fragments (53386+9199496 sites), 322 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     272 bp
  25% of sequence in fragments shorter than     323 bp
  30% of sequence in fragments shorter than     371 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     479 bp
  45% of sequence in fragments shorter than     535 bp
  50% of sequence in fragments shorter than     596 bp
  55% of sequence in fragments shorter than     661 bp
  60% of sequence in fragments shorter than     733 bp
  65% of sequence in fragments shorter than     812 bp
  70% of sequence in fragments shorter than     900 bp
  75% of sequence in fragments shorter than    1003 bp
  80% of sequence in fragments shorter than    1121 bp
  85% of sequence in fragments shorter than    1279 bp
  90% of sequence in fragments shorter than    1498 bp
  95% of sequence in fragments shorter than    1873 bp

# BstEII.HinfI (GGTNACC+GANTC) double digest, 9251 thousand fragments (335865+8916054 sites), 322 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     312 bp
  30% of sequence in fragments shorter than     347 bp
  35% of sequence in fragments shorter than     391 bp
  40% of sequence in fragments shorter than     440 bp
  45% of sequence in fragments shorter than     489 bp
  50% of sequence in fragments shorter than     542 bp
  55% of sequence in fragments shorter than     600 bp
  60% of sequence in fragments shorter than     662 bp
  65% of sequence in fragments shorter than     725 bp
  70% of sequence in fragments shorter than     801 bp
  75% of sequence in fragments shorter than     889 bp
  80% of sequence in fragments shorter than     995 bp
  85% of sequence in fragments shorter than    1127 bp
  90% of sequence in fragments shorter than    1307 bp
  95% of sequence in fragments shorter than    1618 bp

# MboII.PmeI (GAAGA+GTTTAAAC) double digest, 9241 thousand fragments (9199496+42094 sites), 322 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     272 bp
  25% of sequence in fragments shorter than     323 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     480 bp
  45% of sequence in fragments shorter than     536 bp
  50% of sequence in fragments shorter than     597 bp
  55% of sequence in fragments shorter than     662 bp
  60% of sequence in fragments shorter than     734 bp
  65% of sequence in fragments shorter than     813 bp
  70% of sequence in fragments shorter than     901 bp
  75% of sequence in fragments shorter than    1004 bp
  80% of sequence in fragments shorter than    1122 bp
  85% of sequence in fragments shorter than    1281 bp
  90% of sequence in fragments shorter than    1501 bp
  95% of sequence in fragments shorter than    1878 bp

# Hpy188I.SexAI (TCNGA+ACCWGGT) double digest, 9239 thousand fragments (8942153+297900 sites), 322 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     210 bp
  20% of sequence in fragments shorter than     259 bp
  25% of sequence in fragments shorter than     309 bp
  30% of sequence in fragments shorter than     353 bp
  35% of sequence in fragments shorter than     403 bp
  40% of sequence in fragments shorter than     455 bp
  45% of sequence in fragments shorter than     510 bp
  50% of sequence in fragments shorter than     568 bp
  55% of sequence in fragments shorter than     629 bp
  60% of sequence in fragments shorter than     697 bp
  65% of sequence in fragments shorter than     773 bp
  70% of sequence in fragments shorter than     857 bp
  75% of sequence in fragments shorter than     955 bp
  80% of sequence in fragments shorter than    1071 bp
  85% of sequence in fragments shorter than    1217 bp
  90% of sequence in fragments shorter than    1411 bp
  95% of sequence in fragments shorter than    1756 bp

# MboII.SalI (GAAGA+GTCGAC) double digest, 9230 thousand fragments (9199496+31396 sites), 322 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     323 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     480 bp
  45% of sequence in fragments shorter than     537 bp
  50% of sequence in fragments shorter than     597 bp
  55% of sequence in fragments shorter than     663 bp
  60% of sequence in fragments shorter than     735 bp
  65% of sequence in fragments shorter than     814 bp
  70% of sequence in fragments shorter than     902 bp
  75% of sequence in fragments shorter than    1005 bp
  80% of sequence in fragments shorter than    1124 bp
  85% of sequence in fragments shorter than    1282 bp
  90% of sequence in fragments shorter than    1503 bp
  95% of sequence in fragments shorter than    1879 bp

# Hpy188I.KpnI (TCNGA+GGTACC) double digest, 9229 thousand fragments (8942153+287974 sites), 322 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     353 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     455 bp
  45% of sequence in fragments shorter than     511 bp
  50% of sequence in fragments shorter than     569 bp
  55% of sequence in fragments shorter than     631 bp
  60% of sequence in fragments shorter than     700 bp
  65% of sequence in fragments shorter than     776 bp
  70% of sequence in fragments shorter than     860 bp
  75% of sequence in fragments shorter than     959 bp
  80% of sequence in fragments shorter than    1076 bp
  85% of sequence in fragments shorter than    1215 bp
  90% of sequence in fragments shorter than    1418 bp
  95% of sequence in fragments shorter than    1765 bp

# Hpy188I.NheI (TCNGA+GCTAGC) double digest, 9229 thousand fragments (8942153+287384 sites), 322 bp average fragment length
   5% of sequence in fragments shorter than     108 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     211 bp
  20% of sequence in fragments shorter than     260 bp
  25% of sequence in fragments shorter than     309 bp
  30% of sequence in fragments shorter than     354 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     457 bp
  45% of sequence in fragments shorter than     513 bp
  50% of sequence in fragments shorter than     571 bp
  55% of sequence in fragments shorter than     633 bp
  60% of sequence in fragments shorter than     701 bp
  65% of sequence in fragments shorter than     778 bp
  70% of sequence in fragments shorter than     862 bp
  75% of sequence in fragments shorter than     961 bp
  80% of sequence in fragments shorter than    1078 bp
  85% of sequence in fragments shorter than    1225 bp
  90% of sequence in fragments shorter than    1421 bp
  95% of sequence in fragments shorter than    1769 bp

# ApaI.TspRI (GGGCCC+CASTG) double digest, 9225 thousand fragments (460339+8765240 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     362 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     464 bp
  45% of sequence in fragments shorter than     518 bp
  50% of sequence in fragments shorter than     577 bp
  55% of sequence in fragments shorter than     641 bp
  60% of sequence in fragments shorter than     709 bp
  65% of sequence in fragments shorter than     787 bp
  70% of sequence in fragments shorter than     876 bp
  75% of sequence in fragments shorter than     976 bp
  80% of sequence in fragments shorter than    1100 bp
  85% of sequence in fragments shorter than    1262 bp
  90% of sequence in fragments shorter than    1490 bp
  95% of sequence in fragments shorter than    1900 bp

# FokI.Tsp45I (GGATG+GTSAC) double digest, 9222 thousand fragments (5201116+4021761 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     270 bp
  25% of sequence in fragments shorter than     317 bp
  30% of sequence in fragments shorter than     361 bp
  35% of sequence in fragments shorter than     410 bp
  40% of sequence in fragments shorter than     462 bp
  45% of sequence in fragments shorter than     516 bp
  50% of sequence in fragments shorter than     573 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     701 bp
  65% of sequence in fragments shorter than     777 bp
  70% of sequence in fragments shorter than     863 bp
  75% of sequence in fragments shorter than     958 bp
  80% of sequence in fragments shorter than    1078 bp
  85% of sequence in fragments shorter than    1231 bp
  90% of sequence in fragments shorter than    1439 bp
  95% of sequence in fragments shorter than    1814 bp

# BbvI.MlyI (GCAGC+GAGTC) double digest, 9221 thousand fragments (5290051+3931692 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     278 bp
  25% of sequence in fragments shorter than     328 bp
  30% of sequence in fragments shorter than     381 bp
  35% of sequence in fragments shorter than     437 bp
  40% of sequence in fragments shorter than     494 bp
  45% of sequence in fragments shorter than     556 bp
  50% of sequence in fragments shorter than     623 bp
  55% of sequence in fragments shorter than     694 bp
  60% of sequence in fragments shorter than     774 bp
  65% of sequence in fragments shorter than     860 bp
  70% of sequence in fragments shorter than     962 bp
  75% of sequence in fragments shorter than    1079 bp
  80% of sequence in fragments shorter than    1225 bp
  85% of sequence in fragments shorter than    1405 bp
  90% of sequence in fragments shorter than    1657 bp
  95% of sequence in fragments shorter than    2118 bp

# MboII.MluI (GAAGA+ACGCGT) double digest, 9220 thousand fragments (9199496+21415 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     537 bp
  50% of sequence in fragments shorter than     598 bp
  55% of sequence in fragments shorter than     664 bp
  60% of sequence in fragments shorter than     736 bp
  65% of sequence in fragments shorter than     815 bp
  70% of sequence in fragments shorter than     903 bp
  75% of sequence in fragments shorter than    1006 bp
  80% of sequence in fragments shorter than    1124 bp
  85% of sequence in fragments shorter than    1283 bp
  90% of sequence in fragments shorter than    1503 bp
  95% of sequence in fragments shorter than    1879 bp

# MboII.SgrAI (GAAGA+CRCCGGYG) double digest, 9214 thousand fragments (9199496+15270 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     664 bp
  60% of sequence in fragments shorter than     736 bp
  65% of sequence in fragments shorter than     815 bp
  70% of sequence in fragments shorter than     903 bp
  75% of sequence in fragments shorter than    1007 bp
  80% of sequence in fragments shorter than    1125 bp
  85% of sequence in fragments shorter than    1283 bp
  90% of sequence in fragments shorter than    1504 bp
  95% of sequence in fragments shorter than    1880 bp

# MboII.NruI (GAAGA+TCGCGA) double digest, 9213 thousand fragments (9199496+14872 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     598 bp
  55% of sequence in fragments shorter than     664 bp
  60% of sequence in fragments shorter than     736 bp
  65% of sequence in fragments shorter than     815 bp
  70% of sequence in fragments shorter than     903 bp
  75% of sequence in fragments shorter than    1007 bp
  80% of sequence in fragments shorter than    1125 bp
  85% of sequence in fragments shorter than    1284 bp
  90% of sequence in fragments shorter than    1505 bp
  95% of sequence in fragments shorter than    1881 bp

# HinfI.SexAI (GANTC+ACCWGGT) double digest, 9213 thousand fragments (8916054+297900 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     313 bp
  30% of sequence in fragments shorter than     348 bp
  35% of sequence in fragments shorter than     393 bp
  40% of sequence in fragments shorter than     442 bp
  45% of sequence in fragments shorter than     492 bp
  50% of sequence in fragments shorter than     545 bp
  55% of sequence in fragments shorter than     603 bp
  60% of sequence in fragments shorter than     665 bp
  65% of sequence in fragments shorter than     729 bp
  70% of sequence in fragments shorter than     806 bp
  75% of sequence in fragments shorter than     895 bp
  80% of sequence in fragments shorter than    1002 bp
  85% of sequence in fragments shorter than    1135 bp
  90% of sequence in fragments shorter than    1317 bp
  95% of sequence in fragments shorter than    1631 bp

# MboII.PvuI (GAAGA+CGATCG) double digest, 9213 thousand fragments (9199496+14186 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     664 bp
  60% of sequence in fragments shorter than     737 bp
  65% of sequence in fragments shorter than     816 bp
  70% of sequence in fragments shorter than     904 bp
  75% of sequence in fragments shorter than    1008 bp
  80% of sequence in fragments shorter than    1126 bp
  85% of sequence in fragments shorter than    1285 bp
  90% of sequence in fragments shorter than    1506 bp
  95% of sequence in fragments shorter than    1883 bp

# FseI.MboII (GGCCGGCC+GAAGA) double digest, 9212 thousand fragments (13662+9199496 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     664 bp
  60% of sequence in fragments shorter than     736 bp
  65% of sequence in fragments shorter than     816 bp
  70% of sequence in fragments shorter than     903 bp
  75% of sequence in fragments shorter than    1007 bp
  80% of sequence in fragments shorter than    1125 bp
  85% of sequence in fragments shorter than    1283 bp
  90% of sequence in fragments shorter than    1504 bp
  95% of sequence in fragments shorter than    1880 bp

# EcoRV.TspRI (GATATC+CASTG) double digest, 9211 thousand fragments (446473+8765240 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     177 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     409 bp
  40% of sequence in fragments shorter than     460 bp
  45% of sequence in fragments shorter than     512 bp
  50% of sequence in fragments shorter than     568 bp
  55% of sequence in fragments shorter than     630 bp
  60% of sequence in fragments shorter than     694 bp
  65% of sequence in fragments shorter than     769 bp
  70% of sequence in fragments shorter than     853 bp
  75% of sequence in fragments shorter than     948 bp
  80% of sequence in fragments shorter than    1063 bp
  85% of sequence in fragments shorter than    1207 bp
  90% of sequence in fragments shorter than    1414 bp
  95% of sequence in fragments shorter than    1799 bp

# MboII.NotI (GAAGA+GCGGCCGC) double digest, 9208 thousand fragments (9199496+9795 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     428 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     665 bp
  60% of sequence in fragments shorter than     737 bp
  65% of sequence in fragments shorter than     816 bp
  70% of sequence in fragments shorter than     904 bp
  75% of sequence in fragments shorter than    1008 bp
  80% of sequence in fragments shorter than    1126 bp
  85% of sequence in fragments shorter than    1285 bp
  90% of sequence in fragments shorter than    1506 bp
  95% of sequence in fragments shorter than    1883 bp

# MboII.RsrII (GAAGA+CGGWCCG) double digest, 9208 thousand fragments (9199496+9764 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     428 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     665 bp
  60% of sequence in fragments shorter than     737 bp
  65% of sequence in fragments shorter than     816 bp
  70% of sequence in fragments shorter than     904 bp
  75% of sequence in fragments shorter than    1008 bp
  80% of sequence in fragments shorter than    1126 bp
  85% of sequence in fragments shorter than    1285 bp
  90% of sequence in fragments shorter than    1506 bp
  95% of sequence in fragments shorter than    1883 bp

# AflII.HaeIII (CTTAAG+GGCC) double digest, 9205 thousand fragments (643284+8562230 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     287 bp
  25% of sequence in fragments shorter than     343 bp
  30% of sequence in fragments shorter than     396 bp
  35% of sequence in fragments shorter than     459 bp
  40% of sequence in fragments shorter than     523 bp
  45% of sequence in fragments shorter than     593 bp
  50% of sequence in fragments shorter than     671 bp
  55% of sequence in fragments shorter than     752 bp
  60% of sequence in fragments shorter than     846 bp
  65% of sequence in fragments shorter than     944 bp
  70% of sequence in fragments shorter than    1060 bp
  75% of sequence in fragments shorter than    1200 bp
  80% of sequence in fragments shorter than    1363 bp
  85% of sequence in fragments shorter than    1580 bp
  90% of sequence in fragments shorter than    1884 bp
  95% of sequence in fragments shorter than    2418 bp

# AscI.MboII (GGCGCGCC+GAAGA) double digest, 9203 thousand fragments (4751+9199496 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     374 bp
  35% of sequence in fragments shorter than     428 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     665 bp
  60% of sequence in fragments shorter than     737 bp
  65% of sequence in fragments shorter than     817 bp
  70% of sequence in fragments shorter than     905 bp
  75% of sequence in fragments shorter than    1009 bp
  80% of sequence in fragments shorter than    1127 bp
  85% of sequence in fragments shorter than    1286 bp
  90% of sequence in fragments shorter than    1508 bp
  95% of sequence in fragments shorter than    1885 bp

# HinfI.KpnI (GANTC+GGTACC) double digest, 9203 thousand fragments (8916054+287974 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     313 bp
  30% of sequence in fragments shorter than     348 bp
  35% of sequence in fragments shorter than     394 bp
  40% of sequence in fragments shorter than     443 bp
  45% of sequence in fragments shorter than     493 bp
  50% of sequence in fragments shorter than     547 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     667 bp
  65% of sequence in fragments shorter than     732 bp
  70% of sequence in fragments shorter than     808 bp
  75% of sequence in fragments shorter than     898 bp
  80% of sequence in fragments shorter than    1004 bp
  85% of sequence in fragments shorter than    1137 bp
  90% of sequence in fragments shorter than    1320 bp
  95% of sequence in fragments shorter than    1633 bp

# HinfI.NheI (GANTC+GCTAGC) double digest, 9202 thousand fragments (8916054+287384 sites), 323 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     348 bp
  35% of sequence in fragments shorter than     394 bp
  40% of sequence in fragments shorter than     443 bp
  45% of sequence in fragments shorter than     494 bp
  50% of sequence in fragments shorter than     547 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     667 bp
  65% of sequence in fragments shorter than     732 bp
  70% of sequence in fragments shorter than     809 bp
  75% of sequence in fragments shorter than     898 bp
  80% of sequence in fragments shorter than    1005 bp
  85% of sequence in fragments shorter than    1137 bp
  90% of sequence in fragments shorter than    1321 bp
  95% of sequence in fragments shorter than    1634 bp

# HaeII.HaeIII (RGCGCY+GGCC) double digest, 9191 thousand fragments (630101+8562230 sites), 324 bp average fragment length
   5% of sequence in fragments shorter than      93 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     292 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     413 bp
  35% of sequence in fragments shorter than     481 bp
  40% of sequence in fragments shorter than     550 bp
  45% of sequence in fragments shorter than     629 bp
  50% of sequence in fragments shorter than     711 bp
  55% of sequence in fragments shorter than     807 bp
  60% of sequence in fragments shorter than     905 bp
  65% of sequence in fragments shorter than    1023 bp
  70% of sequence in fragments shorter than    1160 bp
  75% of sequence in fragments shorter than    1312 bp
  80% of sequence in fragments shorter than    1508 bp
  85% of sequence in fragments shorter than    1761 bp
  90% of sequence in fragments shorter than    2121 bp
  95% of sequence in fragments shorter than    2751 bp

# AciI.TfiI (CCGC+GAWTC) double digest, 9189 thousand fragments (4206130+4984362 sites), 324 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     230 bp
  20% of sequence in fragments shorter than     289 bp
  25% of sequence in fragments shorter than     343 bp
  30% of sequence in fragments shorter than     393 bp
  35% of sequence in fragments shorter than     454 bp
  40% of sequence in fragments shorter than     515 bp
  45% of sequence in fragments shorter than     581 bp
  50% of sequence in fragments shorter than     650 bp
  55% of sequence in fragments shorter than     720 bp
  60% of sequence in fragments shorter than     799 bp
  65% of sequence in fragments shorter than     885 bp
  70% of sequence in fragments shorter than     986 bp
  75% of sequence in fragments shorter than    1100 bp
  80% of sequence in fragments shorter than    1240 bp
  85% of sequence in fragments shorter than    1417 bp
  90% of sequence in fragments shorter than    1664 bp
  95% of sequence in fragments shorter than    2080 bp

# BsrBI.Hpy188I (CCGCTC+TCNGA) double digest, 9177 thousand fragments (235523+8942153 sites), 324 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     311 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     458 bp
  45% of sequence in fragments shorter than     514 bp
  50% of sequence in fragments shorter than     572 bp
  55% of sequence in fragments shorter than     634 bp
  60% of sequence in fragments shorter than     703 bp
  65% of sequence in fragments shorter than     779 bp
  70% of sequence in fragments shorter than     864 bp
  75% of sequence in fragments shorter than     963 bp
  80% of sequence in fragments shorter than    1080 bp
  85% of sequence in fragments shorter than    1227 bp
  90% of sequence in fragments shorter than    1424 bp
  95% of sequence in fragments shorter than    1771 bp

# Hpy188I.KasI (TCNGA+GGCGCC) double digest, 9172 thousand fragments (8942153+230381 sites), 324 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     310 bp
  30% of sequence in fragments shorter than     353 bp
  35% of sequence in fragments shorter than     403 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     566 bp
  55% of sequence in fragments shorter than     626 bp
  60% of sequence in fragments shorter than     693 bp
  65% of sequence in fragments shorter than     769 bp
  70% of sequence in fragments shorter than     851 bp
  75% of sequence in fragments shorter than     948 bp
  80% of sequence in fragments shorter than    1063 bp
  85% of sequence in fragments shorter than    1205 bp
  90% of sequence in fragments shorter than    1394 bp
  95% of sequence in fragments shorter than    1730 bp

# Hpy188I.SwaI (TCNGA+ATTTAAAT) double digest, 9168 thousand fragments (8942153+226602 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     212 bp
  20% of sequence in fragments shorter than     261 bp
  25% of sequence in fragments shorter than     312 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     458 bp
  45% of sequence in fragments shorter than     513 bp
  50% of sequence in fragments shorter than     571 bp
  55% of sequence in fragments shorter than     633 bp
  60% of sequence in fragments shorter than     701 bp
  65% of sequence in fragments shorter than     777 bp
  70% of sequence in fragments shorter than     861 bp
  75% of sequence in fragments shorter than     960 bp
  80% of sequence in fragments shorter than    1076 bp
  85% of sequence in fragments shorter than    1222 bp
  90% of sequence in fragments shorter than    1417 bp
  95% of sequence in fragments shorter than    1764 bp

# HaeIII.SacI (GGCC+GAGCTC) double digest, 9165 thousand fragments (8562230+603323 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     290 bp
  25% of sequence in fragments shorter than     346 bp
  30% of sequence in fragments shorter than     407 bp
  35% of sequence in fragments shorter than     471 bp
  40% of sequence in fragments shorter than     538 bp
  45% of sequence in fragments shorter than     614 bp
  50% of sequence in fragments shorter than     694 bp
  55% of sequence in fragments shorter than     785 bp
  60% of sequence in fragments shorter than     878 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1117 bp
  75% of sequence in fragments shorter than    1266 bp
  80% of sequence in fragments shorter than    1446 bp
  85% of sequence in fragments shorter than    1686 bp
  90% of sequence in fragments shorter than    2021 bp
  95% of sequence in fragments shorter than    2619 bp

# SpeI.TspRI (ACTAGT+CASTG) double digest, 9165 thousand fragments (400286+8765240 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     463 bp
  45% of sequence in fragments shorter than     516 bp
  50% of sequence in fragments shorter than     573 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     701 bp
  65% of sequence in fragments shorter than     776 bp
  70% of sequence in fragments shorter than     862 bp
  75% of sequence in fragments shorter than     957 bp
  80% of sequence in fragments shorter than    1076 bp
  85% of sequence in fragments shorter than    1230 bp
  90% of sequence in fragments shorter than    1444 bp
  95% of sequence in fragments shorter than    1827 bp

# AvrII.HaeIII (CCTAGG+GGCC) double digest, 9156 thousand fragments (594956+8562230 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     289 bp
  25% of sequence in fragments shorter than     346 bp
  30% of sequence in fragments shorter than     403 bp
  35% of sequence in fragments shorter than     470 bp
  40% of sequence in fragments shorter than     534 bp
  45% of sequence in fragments shorter than     610 bp
  50% of sequence in fragments shorter than     690 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     876 bp
  65% of sequence in fragments shorter than     986 bp
  70% of sequence in fragments shorter than    1113 bp
  75% of sequence in fragments shorter than    1265 bp
  80% of sequence in fragments shorter than    1444 bp
  85% of sequence in fragments shorter than    1686 bp
  90% of sequence in fragments shorter than    2020 bp
  95% of sequence in fragments shorter than    2613 bp

# HpaI.TspRI (GTTAAC+CASTG) double digest, 9154 thousand fragments (389418+8765240 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     362 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     462 bp
  45% of sequence in fragments shorter than     516 bp
  50% of sequence in fragments shorter than     573 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     701 bp
  65% of sequence in fragments shorter than     777 bp
  70% of sequence in fragments shorter than     863 bp
  75% of sequence in fragments shorter than     959 bp
  80% of sequence in fragments shorter than    1077 bp
  85% of sequence in fragments shorter than    1233 bp
  90% of sequence in fragments shorter than    1451 bp
  95% of sequence in fragments shorter than    1844 bp

# BsrBI.HinfI (CCGCTC+GANTC) double digest, 9151 thousand fragments (235523+8916054 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     396 bp
  40% of sequence in fragments shorter than     445 bp
  45% of sequence in fragments shorter than     496 bp
  50% of sequence in fragments shorter than     549 bp
  55% of sequence in fragments shorter than     608 bp
  60% of sequence in fragments shorter than     670 bp
  65% of sequence in fragments shorter than     736 bp
  70% of sequence in fragments shorter than     812 bp
  75% of sequence in fragments shorter than     902 bp
  80% of sequence in fragments shorter than    1010 bp
  85% of sequence in fragments shorter than    1143 bp
  90% of sequence in fragments shorter than    1329 bp
  95% of sequence in fragments shorter than    1646 bp

# HinfI.KasI (GANTC+GGCGCC) double digest, 9145 thousand fragments (8916054+230381 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     396 bp
  40% of sequence in fragments shorter than     446 bp
  45% of sequence in fragments shorter than     497 bp
  50% of sequence in fragments shorter than     551 bp
  55% of sequence in fragments shorter than     610 bp
  60% of sequence in fragments shorter than     672 bp
  65% of sequence in fragments shorter than     738 bp
  70% of sequence in fragments shorter than     815 bp
  75% of sequence in fragments shorter than     906 bp
  80% of sequence in fragments shorter than    1014 bp
  85% of sequence in fragments shorter than    1148 bp
  90% of sequence in fragments shorter than    1334 bp
  95% of sequence in fragments shorter than    1652 bp

# HinfI.SwaI (GANTC+ATTTAAAT) double digest, 9142 thousand fragments (8916054+226602 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     350 bp
  35% of sequence in fragments shorter than     395 bp
  40% of sequence in fragments shorter than     444 bp
  45% of sequence in fragments shorter than     494 bp
  50% of sequence in fragments shorter than     547 bp
  55% of sequence in fragments shorter than     605 bp
  60% of sequence in fragments shorter than     667 bp
  65% of sequence in fragments shorter than     730 bp
  70% of sequence in fragments shorter than     806 bp
  75% of sequence in fragments shorter than     894 bp
  80% of sequence in fragments shorter than    1000 bp
  85% of sequence in fragments shorter than    1132 bp
  90% of sequence in fragments shorter than    1312 bp
  95% of sequence in fragments shorter than    1622 bp

# SapI.TspRI (GCTCTTC+CASTG) double digest, 9141 thousand fragments (377161+8765240 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     364 bp
  35% of sequence in fragments shorter than     413 bp
  40% of sequence in fragments shorter than     464 bp
  45% of sequence in fragments shorter than     518 bp
  50% of sequence in fragments shorter than     576 bp
  55% of sequence in fragments shorter than     639 bp
  60% of sequence in fragments shorter than     706 bp
  65% of sequence in fragments shorter than     784 bp
  70% of sequence in fragments shorter than     870 bp
  75% of sequence in fragments shorter than     967 bp
  80% of sequence in fragments shorter than    1087 bp
  85% of sequence in fragments shorter than    1247 bp
  90% of sequence in fragments shorter than    1473 bp
  95% of sequence in fragments shorter than    1876 bp

# SmaI.TspRI (CCCGGG+CASTG) double digest, 9141 thousand fragments (376940+8765240 sites), 325 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     180 bp
  15% of sequence in fragments shorter than     224 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     370 bp
  35% of sequence in fragments shorter than     420 bp
  40% of sequence in fragments shorter than     473 bp
  45% of sequence in fragments shorter than     529 bp
  50% of sequence in fragments shorter than     588 bp
  55% of sequence in fragments shorter than     653 bp
  60% of sequence in fragments shorter than     722 bp
  65% of sequence in fragments shorter than     801 bp
  70% of sequence in fragments shorter than     892 bp
  75% of sequence in fragments shorter than     992 bp
  80% of sequence in fragments shorter than    1118 bp
  85% of sequence in fragments shorter than    1280 bp
  90% of sequence in fragments shorter than    1503 bp
  95% of sequence in fragments shorter than    1921 bp

# CviQI.Tsp45I (GTAC+GTSAC) double digest, 9136 thousand fragments (5115078+4021761 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     362 bp
  35% of sequence in fragments shorter than     410 bp
  40% of sequence in fragments shorter than     458 bp
  45% of sequence in fragments shorter than     508 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     618 bp
  60% of sequence in fragments shorter than     680 bp
  65% of sequence in fragments shorter than     748 bp
  70% of sequence in fragments shorter than     826 bp
  75% of sequence in fragments shorter than     916 bp
  80% of sequence in fragments shorter than    1026 bp
  85% of sequence in fragments shorter than    1166 bp
  90% of sequence in fragments shorter than    1350 bp
  95% of sequence in fragments shorter than    1665 bp

# HaeIII.HgaI (GGCC+GACGC) double digest, 9133 thousand fragments (8562230+571889 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than      94 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     293 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     412 bp
  35% of sequence in fragments shorter than     479 bp
  40% of sequence in fragments shorter than     548 bp
  45% of sequence in fragments shorter than     624 bp
  50% of sequence in fragments shorter than     705 bp
  55% of sequence in fragments shorter than     799 bp
  60% of sequence in fragments shorter than     895 bp
  65% of sequence in fragments shorter than    1010 bp
  70% of sequence in fragments shorter than    1142 bp
  75% of sequence in fragments shorter than    1294 bp
  80% of sequence in fragments shorter than    1485 bp
  85% of sequence in fragments shorter than    1732 bp
  90% of sequence in fragments shorter than    2084 bp
  95% of sequence in fragments shorter than    2699 bp

# EciI.HaeIII (GGCGGA+GGCC) double digest, 9133 thousand fragments (571273+8562230 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than      96 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     290 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     413 bp
  35% of sequence in fragments shorter than     481 bp
  40% of sequence in fragments shorter than     552 bp
  45% of sequence in fragments shorter than     632 bp
  50% of sequence in fragments shorter than     717 bp
  55% of sequence in fragments shorter than     814 bp
  60% of sequence in fragments shorter than     913 bp
  65% of sequence in fragments shorter than    1032 bp
  70% of sequence in fragments shorter than    1168 bp
  75% of sequence in fragments shorter than    1320 bp
  80% of sequence in fragments shorter than    1518 bp
  85% of sequence in fragments shorter than    1771 bp
  90% of sequence in fragments shorter than    2133 bp
  95% of sequence in fragments shorter than    2770 bp

# FokI.MlyI (GGATG+GAGTC) double digest, 9132 thousand fragments (5201116+3931692 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     413 bp
  40% of sequence in fragments shorter than     466 bp
  45% of sequence in fragments shorter than     520 bp
  50% of sequence in fragments shorter than     580 bp
  55% of sequence in fragments shorter than     642 bp
  60% of sequence in fragments shorter than     712 bp
  65% of sequence in fragments shorter than     789 bp
  70% of sequence in fragments shorter than     875 bp
  75% of sequence in fragments shorter than     974 bp
  80% of sequence in fragments shorter than    1091 bp
  85% of sequence in fragments shorter than    1243 bp
  90% of sequence in fragments shorter than    1458 bp
  95% of sequence in fragments shorter than    1839 bp

# BamHI.TspRI (GGATCC+CASTG) double digest, 9130 thousand fragments (365999+8765240 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     222 bp
  20% of sequence in fragments shorter than     270 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     465 bp
  45% of sequence in fragments shorter than     517 bp
  50% of sequence in fragments shorter than     575 bp
  55% of sequence in fragments shorter than     637 bp
  60% of sequence in fragments shorter than     704 bp
  65% of sequence in fragments shorter than     781 bp
  70% of sequence in fragments shorter than     869 bp
  75% of sequence in fragments shorter than     971 bp
  80% of sequence in fragments shorter than    1094 bp
  85% of sequence in fragments shorter than    1254 bp
  90% of sequence in fragments shorter than    1481 bp
  95% of sequence in fragments shorter than    1884 bp

# ApoI.AvaII (RAATTY+GGWCC) double digest, 9129 thousand fragments (6382433+2747690 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     361 bp
  35% of sequence in fragments shorter than     409 bp
  40% of sequence in fragments shorter than     462 bp
  45% of sequence in fragments shorter than     516 bp
  50% of sequence in fragments shorter than     571 bp
  55% of sequence in fragments shorter than     633 bp
  60% of sequence in fragments shorter than     698 bp
  65% of sequence in fragments shorter than     773 bp
  70% of sequence in fragments shorter than     853 bp
  75% of sequence in fragments shorter than     948 bp
  80% of sequence in fragments shorter than    1063 bp
  85% of sequence in fragments shorter than    1211 bp
  90% of sequence in fragments shorter than    1413 bp
  95% of sequence in fragments shorter than    1780 bp

# HaeIII.MfeI (GGCC+CAATTG) double digest, 9126 thousand fragments (8562230+564303 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     287 bp
  25% of sequence in fragments shorter than     345 bp
  30% of sequence in fragments shorter than     400 bp
  35% of sequence in fragments shorter than     464 bp
  40% of sequence in fragments shorter than     527 bp
  45% of sequence in fragments shorter than     598 bp
  50% of sequence in fragments shorter than     676 bp
  55% of sequence in fragments shorter than     757 bp
  60% of sequence in fragments shorter than     851 bp
  65% of sequence in fragments shorter than     949 bp
  70% of sequence in fragments shorter than    1065 bp
  75% of sequence in fragments shorter than    1204 bp
  80% of sequence in fragments shorter than    1367 bp
  85% of sequence in fragments shorter than    1583 bp
  90% of sequence in fragments shorter than    1888 bp
  95% of sequence in fragments shorter than    2413 bp

# HphI.StyI (GGTGA+CCWWGG) double digest, 9120 thousand fragments (6007336+3114107 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     270 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     468 bp
  45% of sequence in fragments shorter than     522 bp
  50% of sequence in fragments shorter than     581 bp
  55% of sequence in fragments shorter than     645 bp
  60% of sequence in fragments shorter than     715 bp
  65% of sequence in fragments shorter than     793 bp
  70% of sequence in fragments shorter than     880 bp
  75% of sequence in fragments shorter than     989 bp
  80% of sequence in fragments shorter than    1118 bp
  85% of sequence in fragments shorter than    1287 bp
  90% of sequence in fragments shorter than    1513 bp
  95% of sequence in fragments shorter than    1939 bp

# AclI.Hpy188I (AACGTT+TCNGA) double digest, 9114 thousand fragments (172706+8942153 sites), 326 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     262 bp
  25% of sequence in fragments shorter than     313 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     410 bp
  40% of sequence in fragments shorter than     462 bp
  45% of sequence in fragments shorter than     518 bp
  50% of sequence in fragments shorter than     577 bp
  55% of sequence in fragments shorter than     639 bp
  60% of sequence in fragments shorter than     708 bp
  65% of sequence in fragments shorter than     785 bp
  70% of sequence in fragments shorter than     870 bp
  75% of sequence in fragments shorter than     969 bp
  80% of sequence in fragments shorter than    1087 bp
  85% of sequence in fragments shorter than    1235 bp
  90% of sequence in fragments shorter than    1433 bp
  95% of sequence in fragments shorter than    1782 bp

# HaeIII.SphI (GGCC+GCATGC) double digest, 9112 thousand fragments (8562230+550952 sites), 327 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     165 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     289 bp
  25% of sequence in fragments shorter than     346 bp
  30% of sequence in fragments shorter than     406 bp
  35% of sequence in fragments shorter than     471 bp
  40% of sequence in fragments shorter than     537 bp
  45% of sequence in fragments shorter than     612 bp
  50% of sequence in fragments shorter than     692 bp
  55% of sequence in fragments shorter than     782 bp
  60% of sequence in fragments shorter than     874 bp
  65% of sequence in fragments shorter than     985 bp
  70% of sequence in fragments shorter than    1112 bp
  75% of sequence in fragments shorter than    1263 bp
  80% of sequence in fragments shorter than    1439 bp
  85% of sequence in fragments shorter than    1678 bp
  90% of sequence in fragments shorter than    2008 bp
  95% of sequence in fragments shorter than    2600 bp

# HphI.NspI (GGTGA+RCATGY) double digest, 9111 thousand fragments (6007336+3104359 sites), 327 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     168 bp
  15% of sequence in fragments shorter than     220 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     314 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     457 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     565 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     688 bp
  65% of sequence in fragments shorter than     760 bp
  70% of sequence in fragments shorter than     839 bp
  75% of sequence in fragments shorter than     933 bp
  80% of sequence in fragments shorter than    1049 bp
  85% of sequence in fragments shorter than    1199 bp
  90% of sequence in fragments shorter than    1385 bp
  95% of sequence in fragments shorter than    1733 bp

# ApoI.SmlI (RAATTY+CTYRAG) double digest, 9109 thousand fragments (6382433+2727867 sites), 327 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     312 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     408 bp
  40% of sequence in fragments shorter than     461 bp
  45% of sequence in fragments shorter than     518 bp
  50% of sequence in fragments shorter than     577 bp
  55% of sequence in fragments shorter than     641 bp
  60% of sequence in fragments shorter than     710 bp
  65% of sequence in fragments shorter than     788 bp
  70% of sequence in fragments shorter than     878 bp
  75% of sequence in fragments shorter than     976 bp
  80% of sequence in fragments shorter than    1103 bp
  85% of sequence in fragments shorter than    1271 bp
  90% of sequence in fragments shorter than    1515 bp
  95% of sequence in fragments shorter than    1978 bp

# HaeIII.ScaI (GGCC+AGTACT) double digest, 9105 thousand fragments (8562230+543793 sites), 327 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     230 bp
  20% of sequence in fragments shorter than     291 bp
  25% of sequence in fragments shorter than     346 bp
  30% of sequence in fragments shorter than     404 bp
  35% of sequence in fragments shorter than     465 bp
  40% of sequence in fragments shorter than     529 bp
  45% of sequence in fragments shorter than     600 bp
  50% of sequence in fragments shorter than     678 bp
  55% of sequence in fragments shorter than     759 bp
  60% of sequence in fragments shorter than     853 bp
  65% of sequence in fragments shorter than     952 bp
  70% of sequence in fragments shorter than    1069 bp
  75% of sequence in fragments shorter than    1209 bp
  80% of sequence in fragments shorter than    1371 bp
  85% of sequence in fragments shorter than    1587 bp
  90% of sequence in fragments shorter than    1891 bp
  95% of sequence in fragments shorter than    2422 bp

# Hpy188I.PacI (TCNGA+TTAATTAA) double digest, 9101 thousand fragments (8942153+159455 sites), 327 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     314 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     410 bp
  40% of sequence in fragments shorter than     462 bp
  45% of sequence in fragments shorter than     518 bp
  50% of sequence in fragments shorter than     576 bp
  55% of sequence in fragments shorter than     638 bp
  60% of sequence in fragments shorter than     707 bp
  65% of sequence in fragments shorter than     784 bp
  70% of sequence in fragments shorter than     869 bp
  75% of sequence in fragments shorter than     968 bp
  80% of sequence in fragments shorter than    1085 bp
  85% of sequence in fragments shorter than    1233 bp
  90% of sequence in fragments shorter than    1430 bp
  95% of sequence in fragments shorter than    1778 bp

# BstEII.TspRI (GGTNACC+CASTG) double digest, 9100 thousand fragments (335865+8765240 sites), 327 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     179 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     467 bp
  45% of sequence in fragments shorter than     521 bp
  50% of sequence in fragments shorter than     579 bp
  55% of sequence in fragments shorter than     643 bp
  60% of sequence in fragments shorter than     711 bp
  65% of sequence in fragments shorter than     788 bp
  70% of sequence in fragments shorter than     876 bp
  75% of sequence in fragments shorter than     974 bp
  80% of sequence in fragments shorter than    1095 bp
  85% of sequence in fragments shorter than    1256 bp
  90% of sequence in fragments shorter than    1483 bp
  95% of sequence in fragments shorter than    1890 bp

# Hpy188I.Hpy99I (TCNGA+CGWCG) double digest, 9099 thousand fragments (8942153+157917 sites), 327 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     264 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     463 bp
  45% of sequence in fragments shorter than     519 bp
  50% of sequence in fragments shorter than     578 bp
  55% of sequence in fragments shorter than     640 bp
  60% of sequence in fragments shorter than     709 bp
  65% of sequence in fragments shorter than     786 bp
  70% of sequence in fragments shorter than     872 bp
  75% of sequence in fragments shorter than     971 bp
  80% of sequence in fragments shorter than    1090 bp
  85% of sequence in fragments shorter than    1237 bp
  90% of sequence in fragments shorter than    1436 bp
  95% of sequence in fragments shorter than    1787 bp

# AclI.HinfI (AACGTT+GANTC) double digest, 9088 thousand fragments (172706+8916054 sites), 327 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     270 bp
  25% of sequence in fragments shorter than     316 bp
  30% of sequence in fragments shorter than     352 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     448 bp
  45% of sequence in fragments shorter than     499 bp
  50% of sequence in fragments shorter than     553 bp
  55% of sequence in fragments shorter than     611 bp
  60% of sequence in fragments shorter than     673 bp
  65% of sequence in fragments shorter than     739 bp
  70% of sequence in fragments shorter than     815 bp
  75% of sequence in fragments shorter than     905 bp
  80% of sequence in fragments shorter than    1013 bp
  85% of sequence in fragments shorter than    1146 bp
  90% of sequence in fragments shorter than    1330 bp
  95% of sequence in fragments shorter than    1646 bp

# HinfI.PacI (GANTC+TTAATTAA) double digest, 9075 thousand fragments (8916054+159455 sites), 328 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     224 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     317 bp
  30% of sequence in fragments shorter than     353 bp
  35% of sequence in fragments shorter than     399 bp
  40% of sequence in fragments shorter than     448 bp
  45% of sequence in fragments shorter than     499 bp
  50% of sequence in fragments shorter than     553 bp
  55% of sequence in fragments shorter than     611 bp
  60% of sequence in fragments shorter than     673 bp
  65% of sequence in fragments shorter than     739 bp
  70% of sequence in fragments shorter than     815 bp
  75% of sequence in fragments shorter than     905 bp
  80% of sequence in fragments shorter than    1012 bp
  85% of sequence in fragments shorter than    1145 bp
  90% of sequence in fragments shorter than    1329 bp
  95% of sequence in fragments shorter than    1644 bp

# Hpy188I.SnaBI (TCNGA+TACGTA) double digest, 9073 thousand fragments (8942153+132299 sites), 328 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     411 bp
  40% of sequence in fragments shorter than     463 bp
  45% of sequence in fragments shorter than     519 bp
  50% of sequence in fragments shorter than     578 bp
  55% of sequence in fragments shorter than     640 bp
  60% of sequence in fragments shorter than     709 bp
  65% of sequence in fragments shorter than     786 bp
  70% of sequence in fragments shorter than     871 bp
  75% of sequence in fragments shorter than     970 bp
  80% of sequence in fragments shorter than    1088 bp
  85% of sequence in fragments shorter than    1235 bp
  90% of sequence in fragments shorter than    1433 bp
  95% of sequence in fragments shorter than    1782 bp

# HinfI.Hpy99I (GANTC+CGWCG) double digest, 9073 thousand fragments (8916054+157917 sites), 328 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     224 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     353 bp
  35% of sequence in fragments shorter than     400 bp
  40% of sequence in fragments shorter than     449 bp
  45% of sequence in fragments shorter than     501 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     613 bp
  60% of sequence in fragments shorter than     675 bp
  65% of sequence in fragments shorter than     741 bp
  70% of sequence in fragments shorter than     818 bp
  75% of sequence in fragments shorter than     909 bp
  80% of sequence in fragments shorter than    1017 bp
  85% of sequence in fragments shorter than    1152 bp
  90% of sequence in fragments shorter than    1338 bp
  95% of sequence in fragments shorter than    1657 bp

# Hpy188I.NaeI (TCNGA+GCCGGC) double digest, 9067 thousand fragments (8942153+125692 sites), 328 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     464 bp
  45% of sequence in fragments shorter than     520 bp
  50% of sequence in fragments shorter than     579 bp
  55% of sequence in fragments shorter than     641 bp
  60% of sequence in fragments shorter than     711 bp
  65% of sequence in fragments shorter than     788 bp
  70% of sequence in fragments shorter than     874 bp
  75% of sequence in fragments shorter than     973 bp
  80% of sequence in fragments shorter than    1092 bp
  85% of sequence in fragments shorter than    1240 bp
  90% of sequence in fragments shorter than    1440 bp
  95% of sequence in fragments shorter than    1791 bp

# Hpy188I.XhoI (TCNGA+CTCGAG) double digest, 9062 thousand fragments (8942153+121308 sites), 328 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     265 bp
  25% of sequence in fragments shorter than     315 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     412 bp
  40% of sequence in fragments shorter than     464 bp
  45% of sequence in fragments shorter than     520 bp
  50% of sequence in fragments shorter than     579 bp
  55% of sequence in fragments shorter than     641 bp
  60% of sequence in fragments shorter than     710 bp
  65% of sequence in fragments shorter than     788 bp
  70% of sequence in fragments shorter than     873 bp
  75% of sequence in fragments shorter than     973 bp
  80% of sequence in fragments shorter than    1091 bp
  85% of sequence in fragments shorter than    1238 bp
  90% of sequence in fragments shorter than    1436 bp
  95% of sequence in fragments shorter than    1786 bp

# SexAI.TspRI (ACCWGGT+CASTG) double digest, 9062 thousand fragments (297900+8765240 sites), 328 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     179 bp
  15% of sequence in fragments shorter than     224 bp
  20% of sequence in fragments shorter than     271 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     367 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     469 bp
  45% of sequence in fragments shorter than     524 bp
  50% of sequence in fragments shorter than     582 bp
  55% of sequence in fragments shorter than     646 bp
  60% of sequence in fragments shorter than     714 bp
  65% of sequence in fragments shorter than     792 bp
  70% of sequence in fragments shorter than     881 bp
  75% of sequence in fragments shorter than     979 bp
  80% of sequence in fragments shorter than    1102 bp
  85% of sequence in fragments shorter than    1263 bp
  90% of sequence in fragments shorter than    1487 bp
  95% of sequence in fragments shorter than    1893 bp

# ApaLI.HaeIII (GTGCAC+GGCC) double digest, 9057 thousand fragments (496255+8562230 sites), 328 bp average fragment length
   5% of sequence in fragments shorter than      95 bp
  10% of sequence in fragments shorter than     167 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     292 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     410 bp
  35% of sequence in fragments shorter than     475 bp
  40% of sequence in fragments shorter than     543 bp
  45% of sequence in fragments shorter than     618 bp
  50% of sequence in fragments shorter than     698 bp
  55% of sequence in fragments shorter than     786 bp
  60% of sequence in fragments shorter than     879 bp
  65% of sequence in fragments shorter than     992 bp
  70% of sequence in fragments shorter than    1119 bp
  75% of sequence in fragments shorter than    1268 bp
  80% of sequence in fragments shorter than    1449 bp
  85% of sequence in fragments shorter than    1691 bp
  90% of sequence in fragments shorter than    2025 bp
  95% of sequence in fragments shorter than    2622 bp

# KpnI.TspRI (GGTACC+CASTG) double digest, 9052 thousand fragments (287974+8765240 sites), 329 bp average fragment length
   5% of sequence in fragments shorter than     119 bp
  10% of sequence in fragments shorter than     180 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     367 bp
  35% of sequence in fragments shorter than     418 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     525 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     641 bp
  60% of sequence in fragments shorter than     708 bp
  65% of sequence in fragments shorter than     785 bp
  70% of sequence in fragments shorter than     872 bp
  75% of sequence in fragments shorter than     970 bp
  80% of sequence in fragments shorter than    1091 bp
  85% of sequence in fragments shorter than    1250 bp
  90% of sequence in fragments shorter than    1473 bp
  95% of sequence in fragments shorter than    1888 bp

# NheI.TspRI (GCTAGC+CASTG) double digest, 9052 thousand fragments (287384+8765240 sites), 329 bp average fragment length
   5% of sequence in fragments shorter than     119 bp
  10% of sequence in fragments shorter than     180 bp
  15% of sequence in fragments shorter than     224 bp
  20% of sequence in fragments shorter than     272 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     368 bp
  35% of sequence in fragments shorter than     418 bp
  40% of sequence in fragments shorter than     468 bp
  45% of sequence in fragments shorter than     520 bp
  50% of sequence in fragments shorter than     579 bp
  55% of sequence in fragments shorter than     641 bp
  60% of sequence in fragments shorter than     708 bp
  65% of sequence in fragments shorter than     785 bp
  70% of sequence in fragments shorter than     874 bp
  75% of sequence in fragments shorter than     977 bp
  80% of sequence in fragments shorter than    1100 bp
  85% of sequence in fragments shorter than    1255 bp
  90% of sequence in fragments shorter than    1473 bp
  95% of sequence in fragments shorter than    1884 bp

# HinfI.SnaBI (GANTC+TACGTA) double digest, 9047 thousand fragments (8916054+132299 sites), 329 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     272 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     354 bp
  35% of sequence in fragments shorter than     400 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     501 bp
  50% of sequence in fragments shorter than     554 bp
  55% of sequence in fragments shorter than     613 bp
  60% of sequence in fragments shorter than     675 bp
  65% of sequence in fragments shorter than     741 bp
  70% of sequence in fragments shorter than     817 bp
  75% of sequence in fragments shorter than     907 bp
  80% of sequence in fragments shorter than    1015 bp
  85% of sequence in fragments shorter than    1148 bp
  90% of sequence in fragments shorter than    1333 bp
  95% of sequence in fragments shorter than    1649 bp

# CviQI.MlyI (GTAC+GAGTC) double digest, 9046 thousand fragments (5115078+3931692 sites), 329 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     409 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     555 bp
  55% of sequence in fragments shorter than     613 bp
  60% of sequence in fragments shorter than     673 bp
  65% of sequence in fragments shorter than     741 bp
  70% of sequence in fragments shorter than     816 bp
  75% of sequence in fragments shorter than     903 bp
  80% of sequence in fragments shorter than    1012 bp
  85% of sequence in fragments shorter than    1146 bp
  90% of sequence in fragments shorter than    1329 bp
  95% of sequence in fragments shorter than    1644 bp

# HinfI.NaeI (GANTC+GCCGGC) double digest, 9041 thousand fragments (8916054+125692 sites), 329 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     224 bp
  20% of sequence in fragments shorter than     272 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     354 bp
  35% of sequence in fragments shorter than     401 bp
  40% of sequence in fragments shorter than     450 bp
  45% of sequence in fragments shorter than     502 bp
  50% of sequence in fragments shorter than     555 bp
  55% of sequence in fragments shorter than     615 bp
  60% of sequence in fragments shorter than     677 bp
  65% of sequence in fragments shorter than     743 bp
  70% of sequence in fragments shorter than     820 bp
  75% of sequence in fragments shorter than     911 bp
  80% of sequence in fragments shorter than    1020 bp
  85% of sequence in fragments shorter than    1154 bp
  90% of sequence in fragments shorter than    1340 bp
  95% of sequence in fragments shorter than    1660 bp

# BspNCI.HhaI (CCAGA+GCGC) double digest, 9038 thousand fragments (7282436+1756502 sites), 329 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     330 bp
  30% of sequence in fragments shorter than     381 bp
  35% of sequence in fragments shorter than     436 bp
  40% of sequence in fragments shorter than     493 bp
  45% of sequence in fragments shorter than     552 bp
  50% of sequence in fragments shorter than     612 bp
  55% of sequence in fragments shorter than     677 bp
  60% of sequence in fragments shorter than     748 bp
  65% of sequence in fragments shorter than     832 bp
  70% of sequence in fragments shorter than     927 bp
  75% of sequence in fragments shorter than    1037 bp
  80% of sequence in fragments shorter than    1161 bp
  85% of sequence in fragments shorter than    1333 bp
  90% of sequence in fragments shorter than    1572 bp
  95% of sequence in fragments shorter than    1977 bp

# HinfI.XhoI (GANTC+CTCGAG) double digest, 9036 thousand fragments (8916054+121308 sites), 329 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     273 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     355 bp
  35% of sequence in fragments shorter than     402 bp
  40% of sequence in fragments shorter than     451 bp
  45% of sequence in fragments shorter than     503 bp
  50% of sequence in fragments shorter than     557 bp
  55% of sequence in fragments shorter than     616 bp
  60% of sequence in fragments shorter than     678 bp
  65% of sequence in fragments shorter than     745 bp
  70% of sequence in fragments shorter than     822 bp
  75% of sequence in fragments shorter than     913 bp
  80% of sequence in fragments shorter than    1021 bp
  85% of sequence in fragments shorter than    1156 bp
  90% of sequence in fragments shorter than    1344 bp
  95% of sequence in fragments shorter than    1663 bp

# ClaI.Hpy188I (ATCGAT+TCNGA) double digest, 9028 thousand fragments (86477+8942153 sites), 330 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     215 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     317 bp
  30% of sequence in fragments shorter than     361 bp
  35% of sequence in fragments shorter than     413 bp
  40% of sequence in fragments shorter than     466 bp
  45% of sequence in fragments shorter than     522 bp
  50% of sequence in fragments shorter than     581 bp
  55% of sequence in fragments shorter than     644 bp
  60% of sequence in fragments shorter than     714 bp
  65% of sequence in fragments shorter than     791 bp
  70% of sequence in fragments shorter than     877 bp
  75% of sequence in fragments shorter than     977 bp
  80% of sequence in fragments shorter than    1096 bp
  85% of sequence in fragments shorter than    1242 bp
  90% of sequence in fragments shorter than    1444 bp
  95% of sequence in fragments shorter than    1797 bp

# ApaI.HaeIII (GGGCCC+GGCC) double digest, 9022 thousand fragments (460339+8562230 sites), 330 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     581 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     847 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1206 bp
  75% of sequence in fragments shorter than    1363 bp
  80% of sequence in fragments shorter than    1563 bp
  85% of sequence in fragments shorter than    1822 bp
  90% of sequence in fragments shorter than    2191 bp
  95% of sequence in fragments shorter than    2838 bp

# AatII.Hpy188I (GACGTC+TCNGA) double digest, 9012 thousand fragments (71089+8942153 sites), 330 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     467 bp
  45% of sequence in fragments shorter than     524 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     646 bp
  60% of sequence in fragments shorter than     716 bp
  65% of sequence in fragments shorter than     794 bp
  70% of sequence in fragments shorter than     880 bp
  75% of sequence in fragments shorter than     980 bp
  80% of sequence in fragments shorter than    1100 bp
  85% of sequence in fragments shorter than    1247 bp
  90% of sequence in fragments shorter than    1449 bp
  95% of sequence in fragments shorter than    1803 bp

# Hpy188I.SacII (TCNGA+CCGCGG) double digest, 9008 thousand fragments (8942153+66901 sites), 330 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     362 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     467 bp
  45% of sequence in fragments shorter than     524 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     646 bp
  60% of sequence in fragments shorter than     716 bp
  65% of sequence in fragments shorter than     794 bp
  70% of sequence in fragments shorter than     880 bp
  75% of sequence in fragments shorter than     981 bp
  80% of sequence in fragments shorter than    1101 bp
  85% of sequence in fragments shorter than    1249 bp
  90% of sequence in fragments shorter than    1451 bp
  95% of sequence in fragments shorter than    1806 bp

# BsmAI.SspI (GTCTC+AATATT) double digest, 9008 thousand fragments (6631585+2377267 sites), 330 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     370 bp
  35% of sequence in fragments shorter than     421 bp
  40% of sequence in fragments shorter than     475 bp
  45% of sequence in fragments shorter than     529 bp
  50% of sequence in fragments shorter than     587 bp
  55% of sequence in fragments shorter than     649 bp
  60% of sequence in fragments shorter than     718 bp
  65% of sequence in fragments shorter than     797 bp
  70% of sequence in fragments shorter than     886 bp
  75% of sequence in fragments shorter than     991 bp
  80% of sequence in fragments shorter than    1116 bp
  85% of sequence in fragments shorter than    1281 bp
  90% of sequence in fragments shorter than    1511 bp
  95% of sequence in fragments shorter than    1941 bp

# EcoRV.HaeIII (GATATC+GGCC) double digest, 9008 thousand fragments (446473+8562230 sites), 330 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     232 bp
  20% of sequence in fragments shorter than     295 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     407 bp
  35% of sequence in fragments shorter than     471 bp
  40% of sequence in fragments shorter than     536 bp
  45% of sequence in fragments shorter than     608 bp
  50% of sequence in fragments shorter than     686 bp
  55% of sequence in fragments shorter than     769 bp
  60% of sequence in fragments shorter than     861 bp
  65% of sequence in fragments shorter than     961 bp
  70% of sequence in fragments shorter than    1079 bp
  75% of sequence in fragments shorter than    1220 bp
  80% of sequence in fragments shorter than    1390 bp
  85% of sequence in fragments shorter than    1613 bp
  90% of sequence in fragments shorter than    1927 bp
  95% of sequence in fragments shorter than    2473 bp

# TfiI.Tsp45I (GAWTC+GTSAC) double digest, 9005 thousand fragments (4984362+4021761 sites), 330 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     270 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     403 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     504 bp
  50% of sequence in fragments shorter than     558 bp
  55% of sequence in fragments shorter than     617 bp
  60% of sequence in fragments shorter than     679 bp
  65% of sequence in fragments shorter than     746 bp
  70% of sequence in fragments shorter than     823 bp
  75% of sequence in fragments shorter than     914 bp
  80% of sequence in fragments shorter than    1024 bp
  85% of sequence in fragments shorter than    1159 bp
  90% of sequence in fragments shorter than    1347 bp
  95% of sequence in fragments shorter than    1666 bp

# ClaI.HinfI (ATCGAT+GANTC) double digest, 9002 thousand fragments (86477+8916054 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     356 bp
  35% of sequence in fragments shorter than     403 bp
  40% of sequence in fragments shorter than     452 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     618 bp
  60% of sequence in fragments shorter than     680 bp
  65% of sequence in fragments shorter than     747 bp
  70% of sequence in fragments shorter than     824 bp
  75% of sequence in fragments shorter than     916 bp
  80% of sequence in fragments shorter than    1024 bp
  85% of sequence in fragments shorter than    1158 bp
  90% of sequence in fragments shorter than    1345 bp
  95% of sequence in fragments shorter than    1665 bp

# Hpy188I.PspXI (TCNGA+VCTCGAGB) double digest, 9000 thousand fragments (8942153+59193 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     467 bp
  45% of sequence in fragments shorter than     524 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     646 bp
  60% of sequence in fragments shorter than     716 bp
  65% of sequence in fragments shorter than     794 bp
  70% of sequence in fragments shorter than     880 bp
  75% of sequence in fragments shorter than     980 bp
  80% of sequence in fragments shorter than    1100 bp
  85% of sequence in fragments shorter than    1247 bp
  90% of sequence in fragments shorter than    1449 bp
  95% of sequence in fragments shorter than    1803 bp

# BsrBI.TspRI (CCGCTC+CASTG) double digest, 9000 thousand fragments (235523+8765240 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     119 bp
  10% of sequence in fragments shorter than     181 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     323 bp
  30% of sequence in fragments shorter than     370 bp
  35% of sequence in fragments shorter than     421 bp
  40% of sequence in fragments shorter than     474 bp
  45% of sequence in fragments shorter than     529 bp
  50% of sequence in fragments shorter than     589 bp
  55% of sequence in fragments shorter than     653 bp
  60% of sequence in fragments shorter than     722 bp
  65% of sequence in fragments shorter than     801 bp
  70% of sequence in fragments shorter than     890 bp
  75% of sequence in fragments shorter than     990 bp
  80% of sequence in fragments shorter than    1116 bp
  85% of sequence in fragments shorter than    1277 bp
  90% of sequence in fragments shorter than    1501 bp
  95% of sequence in fragments shorter than    1910 bp

# KasI.TspRI (GGCGCC+CASTG) double digest, 8995 thousand fragments (230381+8765240 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     180 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     275 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     424 bp
  40% of sequence in fragments shorter than     477 bp
  45% of sequence in fragments shorter than     533 bp
  50% of sequence in fragments shorter than     593 bp
  55% of sequence in fragments shorter than     658 bp
  60% of sequence in fragments shorter than     728 bp
  65% of sequence in fragments shorter than     807 bp
  70% of sequence in fragments shorter than     898 bp
  75% of sequence in fragments shorter than     998 bp
  80% of sequence in fragments shorter than    1124 bp
  85% of sequence in fragments shorter than    1287 bp
  90% of sequence in fragments shorter than    1508 bp
  95% of sequence in fragments shorter than    1930 bp

# AgeI.Hpy188I (ACCGGT+TCNGA) double digest, 8995 thousand fragments (53386+8942153 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     216 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     318 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     415 bp
  40% of sequence in fragments shorter than     467 bp
  45% of sequence in fragments shorter than     524 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     647 bp
  60% of sequence in fragments shorter than     717 bp
  65% of sequence in fragments shorter than     795 bp
  70% of sequence in fragments shorter than     881 bp
  75% of sequence in fragments shorter than     982 bp
  80% of sequence in fragments shorter than    1102 bp
  85% of sequence in fragments shorter than    1249 bp
  90% of sequence in fragments shorter than    1452 bp
  95% of sequence in fragments shorter than    1806 bp

# SwaI.TspRI (ATTTAAAT+CASTG) double digest, 8991 thousand fragments (226602+8765240 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     181 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     369 bp
  35% of sequence in fragments shorter than     419 bp
  40% of sequence in fragments shorter than     471 bp
  45% of sequence in fragments shorter than     525 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     645 bp
  60% of sequence in fragments shorter than     713 bp
  65% of sequence in fragments shorter than     790 bp
  70% of sequence in fragments shorter than     877 bp
  75% of sequence in fragments shorter than     974 bp
  80% of sequence in fragments shorter than    1095 bp
  85% of sequence in fragments shorter than    1253 bp
  90% of sequence in fragments shorter than    1474 bp
  95% of sequence in fragments shorter than    1869 bp

# BfiI.BspNCI (ACTGGG+CCAGA) double digest, 8988 thousand fragments (1706594+7282436 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     328 bp
  30% of sequence in fragments shorter than     376 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     597 bp
  55% of sequence in fragments shorter than     662 bp
  60% of sequence in fragments shorter than     732 bp
  65% of sequence in fragments shorter than     814 bp
  70% of sequence in fragments shorter than     903 bp
  75% of sequence in fragments shorter than    1012 bp
  80% of sequence in fragments shorter than    1138 bp
  85% of sequence in fragments shorter than    1294 bp
  90% of sequence in fragments shorter than    1526 bp
  95% of sequence in fragments shorter than    1929 bp

# AatII.HinfI (GACGTC+GANTC) double digest, 8986 thousand fragments (71089+8916054 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     357 bp
  35% of sequence in fragments shorter than     403 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     559 bp
  55% of sequence in fragments shorter than     619 bp
  60% of sequence in fragments shorter than     681 bp
  65% of sequence in fragments shorter than     748 bp
  70% of sequence in fragments shorter than     825 bp
  75% of sequence in fragments shorter than     917 bp
  80% of sequence in fragments shorter than    1025 bp
  85% of sequence in fragments shorter than    1160 bp
  90% of sequence in fragments shorter than    1348 bp
  95% of sequence in fragments shorter than    1668 bp

# Hpy188I.PmeI (TCNGA+GTTTAAAC) double digest, 8983 thousand fragments (8942153+42094 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     267 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     416 bp
  40% of sequence in fragments shorter than     468 bp
  45% of sequence in fragments shorter than     525 bp
  50% of sequence in fragments shorter than     583 bp
  55% of sequence in fragments shorter than     648 bp
  60% of sequence in fragments shorter than     718 bp
  65% of sequence in fragments shorter than     796 bp
  70% of sequence in fragments shorter than     882 bp
  75% of sequence in fragments shorter than     983 bp
  80% of sequence in fragments shorter than    1102 bp
  85% of sequence in fragments shorter than    1250 bp
  90% of sequence in fragments shorter than    1453 bp
  95% of sequence in fragments shorter than    1808 bp

# HinfI.SacII (GANTC+CCGCGG) double digest, 8982 thousand fragments (8916054+66901 sites), 331 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     357 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     506 bp
  50% of sequence in fragments shorter than     560 bp
  55% of sequence in fragments shorter than     619 bp
  60% of sequence in fragments shorter than     681 bp
  65% of sequence in fragments shorter than     748 bp
  70% of sequence in fragments shorter than     826 bp
  75% of sequence in fragments shorter than     918 bp
  80% of sequence in fragments shorter than    1027 bp
  85% of sequence in fragments shorter than    1161 bp
  90% of sequence in fragments shorter than    1349 bp
  95% of sequence in fragments shorter than    1670 bp

# HinfI.PspXI (GANTC+VCTCGAGB) double digest, 8974 thousand fragments (8916054+59193 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     275 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     357 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     506 bp
  50% of sequence in fragments shorter than     561 bp
  55% of sequence in fragments shorter than     620 bp
  60% of sequence in fragments shorter than     682 bp
  65% of sequence in fragments shorter than     749 bp
  70% of sequence in fragments shorter than     827 bp
  75% of sequence in fragments shorter than     919 bp
  80% of sequence in fragments shorter than    1028 bp
  85% of sequence in fragments shorter than    1162 bp
  90% of sequence in fragments shorter than    1350 bp
  95% of sequence in fragments shorter than    1671 bp

# Hpy188I.SalI (TCNGA+GTCGAC) double digest, 8973 thousand fragments (8942153+31396 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     364 bp
  35% of sequence in fragments shorter than     416 bp
  40% of sequence in fragments shorter than     469 bp
  45% of sequence in fragments shorter than     526 bp
  50% of sequence in fragments shorter than     584 bp
  55% of sequence in fragments shorter than     649 bp
  60% of sequence in fragments shorter than     719 bp
  65% of sequence in fragments shorter than     797 bp
  70% of sequence in fragments shorter than     883 bp
  75% of sequence in fragments shorter than     984 bp
  80% of sequence in fragments shorter than    1104 bp
  85% of sequence in fragments shorter than    1251 bp
  90% of sequence in fragments shorter than    1455 bp
  95% of sequence in fragments shorter than    1810 bp

# AgeI.HinfI (ACCGGT+GANTC) double digest, 8968 thousand fragments (53386+8916054 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     275 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     357 bp
  35% of sequence in fragments shorter than     404 bp
  40% of sequence in fragments shorter than     453 bp
  45% of sequence in fragments shorter than     506 bp
  50% of sequence in fragments shorter than     560 bp
  55% of sequence in fragments shorter than     620 bp
  60% of sequence in fragments shorter than     682 bp
  65% of sequence in fragments shorter than     749 bp
  70% of sequence in fragments shorter than     827 bp
  75% of sequence in fragments shorter than     919 bp
  80% of sequence in fragments shorter than    1028 bp
  85% of sequence in fragments shorter than    1162 bp
  90% of sequence in fragments shorter than    1350 bp
  95% of sequence in fragments shorter than    1670 bp

# Hpy188I.MluI (TCNGA+ACGCGT) double digest, 8963 thousand fragments (8942153+21415 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     319 bp
  30% of sequence in fragments shorter than     364 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     469 bp
  45% of sequence in fragments shorter than     526 bp
  50% of sequence in fragments shorter than     584 bp
  55% of sequence in fragments shorter than     649 bp
  60% of sequence in fragments shorter than     720 bp
  65% of sequence in fragments shorter than     798 bp
  70% of sequence in fragments shorter than     884 bp
  75% of sequence in fragments shorter than     985 bp
  80% of sequence in fragments shorter than    1105 bp
  85% of sequence in fragments shorter than    1252 bp
  90% of sequence in fragments shorter than    1456 bp
  95% of sequence in fragments shorter than    1811 bp

# HaeIII.SpeI (GGCC+ACTAGT) double digest, 8962 thousand fragments (8562230+400286 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     233 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     348 bp
  30% of sequence in fragments shorter than     412 bp
  35% of sequence in fragments shorter than     474 bp
  40% of sequence in fragments shorter than     540 bp
  45% of sequence in fragments shorter than     615 bp
  50% of sequence in fragments shorter than     693 bp
  55% of sequence in fragments shorter than     781 bp
  60% of sequence in fragments shorter than     871 bp
  65% of sequence in fragments shorter than     979 bp
  70% of sequence in fragments shorter than    1100 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1416 bp
  85% of sequence in fragments shorter than    1645 bp
  90% of sequence in fragments shorter than    1963 bp
  95% of sequence in fragments shorter than    2528 bp

# HinfI.PmeI (GANTC+GTTTAAAC) double digest, 8957 thousand fragments (8916054+42094 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     275 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     405 bp
  40% of sequence in fragments shorter than     454 bp
  45% of sequence in fragments shorter than     507 bp
  50% of sequence in fragments shorter than     561 bp
  55% of sequence in fragments shorter than     620 bp
  60% of sequence in fragments shorter than     682 bp
  65% of sequence in fragments shorter than     749 bp
  70% of sequence in fragments shorter than     827 bp
  75% of sequence in fragments shorter than     919 bp
  80% of sequence in fragments shorter than    1028 bp
  85% of sequence in fragments shorter than    1162 bp
  90% of sequence in fragments shorter than    1350 bp
  95% of sequence in fragments shorter than    1670 bp

# AvaII.BthCI (GGWCC+GCNGC) double digest, 8957 thousand fragments (2747690+6209929 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     105 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     243 bp
  20% of sequence in fragments shorter than     306 bp
  25% of sequence in fragments shorter than     371 bp
  30% of sequence in fragments shorter than     442 bp
  35% of sequence in fragments shorter than     517 bp
  40% of sequence in fragments shorter than     594 bp
  45% of sequence in fragments shorter than     677 bp
  50% of sequence in fragments shorter than     760 bp
  55% of sequence in fragments shorter than     854 bp
  60% of sequence in fragments shorter than     951 bp
  65% of sequence in fragments shorter than    1067 bp
  70% of sequence in fragments shorter than    1203 bp
  75% of sequence in fragments shorter than    1359 bp
  80% of sequence in fragments shorter than    1551 bp
  85% of sequence in fragments shorter than    1796 bp
  90% of sequence in fragments shorter than    2140 bp
  95% of sequence in fragments shorter than    2740 bp

# Hpy188I.SgrAI (TCNGA+CRCCGGYG) double digest, 8956 thousand fragments (8942153+15270 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     585 bp
  55% of sequence in fragments shorter than     650 bp
  60% of sequence in fragments shorter than     720 bp
  65% of sequence in fragments shorter than     798 bp
  70% of sequence in fragments shorter than     885 bp
  75% of sequence in fragments shorter than     986 bp
  80% of sequence in fragments shorter than    1106 bp
  85% of sequence in fragments shorter than    1252 bp
  90% of sequence in fragments shorter than    1457 bp
  95% of sequence in fragments shorter than    1813 bp

# Hpy188I.NruI (TCNGA+TCGCGA) double digest, 8956 thousand fragments (8942153+14872 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     585 bp
  55% of sequence in fragments shorter than     650 bp
  60% of sequence in fragments shorter than     720 bp
  65% of sequence in fragments shorter than     798 bp
  70% of sequence in fragments shorter than     885 bp
  75% of sequence in fragments shorter than     986 bp
  80% of sequence in fragments shorter than    1105 bp
  85% of sequence in fragments shorter than    1252 bp
  90% of sequence in fragments shorter than    1457 bp
  95% of sequence in fragments shorter than    1812 bp

# Hpy188I.PvuI (TCNGA+CGATCG) double digest, 8955 thousand fragments (8942153+14186 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     585 bp
  55% of sequence in fragments shorter than     650 bp
  60% of sequence in fragments shorter than     720 bp
  65% of sequence in fragments shorter than     799 bp
  70% of sequence in fragments shorter than     885 bp
  75% of sequence in fragments shorter than     986 bp
  80% of sequence in fragments shorter than    1106 bp
  85% of sequence in fragments shorter than    1252 bp
  90% of sequence in fragments shorter than    1458 bp
  95% of sequence in fragments shorter than    1813 bp

# BspKT6I.HhaI (GATC+GCGC) double digest, 8955 thousand fragments (7199434+1756502 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     329 bp
  30% of sequence in fragments shorter than     378 bp
  35% of sequence in fragments shorter than     431 bp
  40% of sequence in fragments shorter than     484 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     604 bp
  55% of sequence in fragments shorter than     670 bp
  60% of sequence in fragments shorter than     739 bp
  65% of sequence in fragments shorter than     819 bp
  70% of sequence in fragments shorter than     909 bp
  75% of sequence in fragments shorter than    1014 bp
  80% of sequence in fragments shorter than    1132 bp
  85% of sequence in fragments shorter than    1292 bp
  90% of sequence in fragments shorter than    1514 bp
  95% of sequence in fragments shorter than    1888 bp

# FseI.Hpy188I (GGCCGGCC+TCNGA) double digest, 8955 thousand fragments (13662+8942153 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     585 bp
  55% of sequence in fragments shorter than     650 bp
  60% of sequence in fragments shorter than     720 bp
  65% of sequence in fragments shorter than     798 bp
  70% of sequence in fragments shorter than     885 bp
  75% of sequence in fragments shorter than     986 bp
  80% of sequence in fragments shorter than    1106 bp
  85% of sequence in fragments shorter than    1252 bp
  90% of sequence in fragments shorter than    1458 bp
  95% of sequence in fragments shorter than    1813 bp

# Hpy188I.NotI (TCNGA+GCGGCCGC) double digest, 8951 thousand fragments (8942153+9795 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     585 bp
  55% of sequence in fragments shorter than     650 bp
  60% of sequence in fragments shorter than     721 bp
  65% of sequence in fragments shorter than     799 bp
  70% of sequence in fragments shorter than     885 bp
  75% of sequence in fragments shorter than     986 bp
  80% of sequence in fragments shorter than    1106 bp
  85% of sequence in fragments shorter than    1253 bp
  90% of sequence in fragments shorter than    1458 bp
  95% of sequence in fragments shorter than    1814 bp

# Hpy188I.RsrII (TCNGA+CGGWCCG) double digest, 8951 thousand fragments (8942153+9764 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     217 bp
  20% of sequence in fragments shorter than     268 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     585 bp
  55% of sequence in fragments shorter than     650 bp
  60% of sequence in fragments shorter than     721 bp
  65% of sequence in fragments shorter than     799 bp
  70% of sequence in fragments shorter than     886 bp
  75% of sequence in fragments shorter than     987 bp
  80% of sequence in fragments shorter than    1107 bp
  85% of sequence in fragments shorter than    1253 bp
  90% of sequence in fragments shorter than    1459 bp
  95% of sequence in fragments shorter than    1815 bp

# HaeIII.HpaI (GGCC+GTTAAC) double digest, 8951 thousand fragments (8562230+389418 sites), 332 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     234 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     348 bp
  30% of sequence in fragments shorter than     411 bp
  35% of sequence in fragments shorter than     476 bp
  40% of sequence in fragments shorter than     541 bp
  45% of sequence in fragments shorter than     615 bp
  50% of sequence in fragments shorter than     693 bp
  55% of sequence in fragments shorter than     781 bp
  60% of sequence in fragments shorter than     871 bp
  65% of sequence in fragments shorter than     979 bp
  70% of sequence in fragments shorter than    1098 bp
  75% of sequence in fragments shorter than    1244 bp
  80% of sequence in fragments shorter than    1416 bp
  85% of sequence in fragments shorter than    1646 bp
  90% of sequence in fragments shorter than    1966 bp
  95% of sequence in fragments shorter than    2532 bp

# BsmAI.HpaII (GTCTC+CCGG) double digest, 8948 thousand fragments (6631585+2317722 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     163 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     287 bp
  25% of sequence in fragments shorter than     354 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     489 bp
  40% of sequence in fragments shorter than     559 bp
  45% of sequence in fragments shorter than     633 bp
  50% of sequence in fragments shorter than     715 bp
  55% of sequence in fragments shorter than     806 bp
  60% of sequence in fragments shorter than     907 bp
  65% of sequence in fragments shorter than    1023 bp
  70% of sequence in fragments shorter than    1153 bp
  75% of sequence in fragments shorter than    1307 bp
  80% of sequence in fragments shorter than    1497 bp
  85% of sequence in fragments shorter than    1741 bp
  90% of sequence in fragments shorter than    2088 bp
  95% of sequence in fragments shorter than    2715 bp

# HinfI.SalI (GANTC+GTCGAC) double digest, 8946 thousand fragments (8916054+31396 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     405 bp
  40% of sequence in fragments shorter than     455 bp
  45% of sequence in fragments shorter than     508 bp
  50% of sequence in fragments shorter than     562 bp
  55% of sequence in fragments shorter than     622 bp
  60% of sequence in fragments shorter than     684 bp
  65% of sequence in fragments shorter than     751 bp
  70% of sequence in fragments shorter than     829 bp
  75% of sequence in fragments shorter than     921 bp
  80% of sequence in fragments shorter than    1030 bp
  85% of sequence in fragments shorter than    1165 bp
  90% of sequence in fragments shorter than    1354 bp
  95% of sequence in fragments shorter than    1676 bp

# AscI.Hpy188I (GGCGCGCC+TCNGA) double digest, 8946 thousand fragments (4751+8942153 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     218 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     320 bp
  30% of sequence in fragments shorter than     365 bp
  35% of sequence in fragments shorter than     417 bp
  40% of sequence in fragments shorter than     470 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     585 bp
  55% of sequence in fragments shorter than     651 bp
  60% of sequence in fragments shorter than     721 bp
  65% of sequence in fragments shorter than     799 bp
  70% of sequence in fragments shorter than     886 bp
  75% of sequence in fragments shorter than     987 bp
  80% of sequence in fragments shorter than    1107 bp
  85% of sequence in fragments shorter than    1253 bp
  90% of sequence in fragments shorter than    1459 bp
  95% of sequence in fragments shorter than    1815 bp

# BbvI.BscAI (GCAGC+GCATC) double digest, 8942 thousand fragments (5290051+3652926 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     177 bp
  15% of sequence in fragments shorter than     236 bp
  20% of sequence in fragments shorter than     293 bp
  25% of sequence in fragments shorter than     348 bp
  30% of sequence in fragments shorter than     408 bp
  35% of sequence in fragments shorter than     470 bp
  40% of sequence in fragments shorter than     535 bp
  45% of sequence in fragments shorter than     603 bp
  50% of sequence in fragments shorter than     671 bp
  55% of sequence in fragments shorter than     748 bp
  60% of sequence in fragments shorter than     832 bp
  65% of sequence in fragments shorter than     925 bp
  70% of sequence in fragments shorter than    1034 bp
  75% of sequence in fragments shorter than    1160 bp
  80% of sequence in fragments shorter than    1307 bp
  85% of sequence in fragments shorter than    1502 bp
  90% of sequence in fragments shorter than    1762 bp
  95% of sequence in fragments shorter than    2233 bp

# HaeIII.SapI (GGCC+GCTCTTC) double digest, 8938 thousand fragments (8562230+377161 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     234 bp
  20% of sequence in fragments shorter than     298 bp
  25% of sequence in fragments shorter than     350 bp
  30% of sequence in fragments shorter than     417 bp
  35% of sequence in fragments shorter than     483 bp
  40% of sequence in fragments shorter than     551 bp
  45% of sequence in fragments shorter than     627 bp
  50% of sequence in fragments shorter than     708 bp
  55% of sequence in fragments shorter than     800 bp
  60% of sequence in fragments shorter than     895 bp
  65% of sequence in fragments shorter than    1009 bp
  70% of sequence in fragments shorter than    1137 bp
  75% of sequence in fragments shorter than    1281 bp
  80% of sequence in fragments shorter than    1467 bp
  85% of sequence in fragments shorter than    1711 bp
  90% of sequence in fragments shorter than    2055 bp
  95% of sequence in fragments shorter than    2664 bp

# HaeIII.SmaI (GGCC+CCCGGG) double digest, 8938 thousand fragments (8562230+376940 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     166 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     351 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     490 bp
  40% of sequence in fragments shorter than     561 bp
  45% of sequence in fragments shorter than     642 bp
  50% of sequence in fragments shorter than     728 bp
  55% of sequence in fragments shorter than     826 bp
  60% of sequence in fragments shorter than     926 bp
  65% of sequence in fragments shorter than    1043 bp
  70% of sequence in fragments shorter than    1183 bp
  75% of sequence in fragments shorter than    1338 bp
  80% of sequence in fragments shorter than    1537 bp
  85% of sequence in fragments shorter than    1793 bp
  90% of sequence in fragments shorter than    2158 bp
  95% of sequence in fragments shorter than    2800 bp

# AclI.TspRI (AACGTT+CASTG) double digest, 8937 thousand fragments (172706+8765240 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     183 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     422 bp
  40% of sequence in fragments shorter than     475 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     588 bp
  55% of sequence in fragments shorter than     651 bp
  60% of sequence in fragments shorter than     720 bp
  65% of sequence in fragments shorter than     797 bp
  70% of sequence in fragments shorter than     886 bp
  75% of sequence in fragments shorter than     986 bp
  80% of sequence in fragments shorter than    1107 bp
  85% of sequence in fragments shorter than    1267 bp
  90% of sequence in fragments shorter than    1489 bp
  95% of sequence in fragments shorter than    1884 bp

# BthCI.SmlI (GCNGC+CTYRAG) double digest, 8937 thousand fragments (6209929+2727867 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     177 bp
  15% of sequence in fragments shorter than     240 bp
  20% of sequence in fragments shorter than     300 bp
  25% of sequence in fragments shorter than     357 bp
  30% of sequence in fragments shorter than     418 bp
  35% of sequence in fragments shorter than     482 bp
  40% of sequence in fragments shorter than     545 bp
  45% of sequence in fragments shorter than     615 bp
  50% of sequence in fragments shorter than     689 bp
  55% of sequence in fragments shorter than     768 bp
  60% of sequence in fragments shorter than     856 bp
  65% of sequence in fragments shorter than     951 bp
  70% of sequence in fragments shorter than    1064 bp
  75% of sequence in fragments shorter than    1195 bp
  80% of sequence in fragments shorter than    1353 bp
  85% of sequence in fragments shorter than    1562 bp
  90% of sequence in fragments shorter than    1852 bp
  95% of sequence in fragments shorter than    2334 bp

# HinfI.MluI (GANTC+ACGCGT) double digest, 8936 thousand fragments (8916054+21415 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     455 bp
  45% of sequence in fragments shorter than     508 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     622 bp
  60% of sequence in fragments shorter than     684 bp
  65% of sequence in fragments shorter than     752 bp
  70% of sequence in fragments shorter than     830 bp
  75% of sequence in fragments shorter than     922 bp
  80% of sequence in fragments shorter than    1031 bp
  85% of sequence in fragments shorter than    1166 bp
  90% of sequence in fragments shorter than    1354 bp
  95% of sequence in fragments shorter than    1677 bp

# HinfI.SgrAI (GANTC+CRCCGGYG) double digest, 8930 thousand fragments (8916054+15270 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     455 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     752 bp
  70% of sequence in fragments shorter than     830 bp
  75% of sequence in fragments shorter than     922 bp
  80% of sequence in fragments shorter than    1031 bp
  85% of sequence in fragments shorter than    1166 bp
  90% of sequence in fragments shorter than    1355 bp
  95% of sequence in fragments shorter than    1677 bp

# HinfI.NruI (GANTC+TCGCGA) double digest, 8930 thousand fragments (8916054+14872 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     753 bp
  70% of sequence in fragments shorter than     831 bp
  75% of sequence in fragments shorter than     923 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1167 bp
  90% of sequence in fragments shorter than    1356 bp
  95% of sequence in fragments shorter than    1679 bp

# BspNCI.PsiI (CCAGA+TTATAA) double digest, 8929 thousand fragments (7282436+1647912 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     324 bp
  30% of sequence in fragments shorter than     371 bp
  35% of sequence in fragments shorter than     420 bp
  40% of sequence in fragments shorter than     471 bp
  45% of sequence in fragments shorter than     524 bp
  50% of sequence in fragments shorter than     579 bp
  55% of sequence in fragments shorter than     636 bp
  60% of sequence in fragments shorter than     700 bp
  65% of sequence in fragments shorter than     771 bp
  70% of sequence in fragments shorter than     855 bp
  75% of sequence in fragments shorter than     947 bp
  80% of sequence in fragments shorter than    1060 bp
  85% of sequence in fragments shorter than    1196 bp
  90% of sequence in fragments shorter than    1396 bp
  95% of sequence in fragments shorter than    1742 bp

# HinfI.PvuI (GANTC+CGATCG) double digest, 8929 thousand fragments (8916054+14186 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     753 bp
  70% of sequence in fragments shorter than     831 bp
  75% of sequence in fragments shorter than     923 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1167 bp
  90% of sequence in fragments shorter than    1356 bp
  95% of sequence in fragments shorter than    1679 bp

# FseI.HinfI (GGCCGGCC+GANTC) double digest, 8929 thousand fragments (13662+8916054 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     752 bp
  70% of sequence in fragments shorter than     830 bp
  75% of sequence in fragments shorter than     922 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1167 bp
  90% of sequence in fragments shorter than    1355 bp
  95% of sequence in fragments shorter than    1677 bp

# BamHI.HaeIII (GGATCC+GGCC) double digest, 8927 thousand fragments (365999+8562230 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     170 bp
  15% of sequence in fragments shorter than     234 bp
  20% of sequence in fragments shorter than     298 bp
  25% of sequence in fragments shorter than     351 bp
  30% of sequence in fragments shorter than     416 bp
  35% of sequence in fragments shorter than     480 bp
  40% of sequence in fragments shorter than     549 bp
  45% of sequence in fragments shorter than     626 bp
  50% of sequence in fragments shorter than     708 bp
  55% of sequence in fragments shorter than     802 bp
  60% of sequence in fragments shorter than     901 bp
  65% of sequence in fragments shorter than    1017 bp
  70% of sequence in fragments shorter than    1146 bp
  75% of sequence in fragments shorter than    1296 bp
  80% of sequence in fragments shorter than    1487 bp
  85% of sequence in fragments shorter than    1733 bp
  90% of sequence in fragments shorter than    2082 bp
  95% of sequence in fragments shorter than    2699 bp

# HinfI.NotI (GANTC+GCGGCCGC) double digest, 8925 thousand fragments (8916054+9795 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     753 bp
  70% of sequence in fragments shorter than     831 bp
  75% of sequence in fragments shorter than     923 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1167 bp
  90% of sequence in fragments shorter than    1356 bp
  95% of sequence in fragments shorter than    1678 bp

# HinfI.RsrII (GANTC+CGGWCCG) double digest, 8925 thousand fragments (8916054+9764 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     321 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     406 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     563 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     753 bp
  70% of sequence in fragments shorter than     831 bp
  75% of sequence in fragments shorter than     923 bp
  80% of sequence in fragments shorter than    1032 bp
  85% of sequence in fragments shorter than    1168 bp
  90% of sequence in fragments shorter than    1356 bp
  95% of sequence in fragments shorter than    1679 bp

# PacI.TspRI (TTAATTAA+CASTG) double digest, 8924 thousand fragments (159455+8765240 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     183 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     325 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     423 bp
  40% of sequence in fragments shorter than     476 bp
  45% of sequence in fragments shorter than     531 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     654 bp
  60% of sequence in fragments shorter than     722 bp
  65% of sequence in fragments shorter than     800 bp
  70% of sequence in fragments shorter than     890 bp
  75% of sequence in fragments shorter than     989 bp
  80% of sequence in fragments shorter than    1112 bp
  85% of sequence in fragments shorter than    1273 bp
  90% of sequence in fragments shorter than    1496 bp
  95% of sequence in fragments shorter than    1901 bp

# Hpy99I.TspRI (CGWCG+CASTG) double digest, 8922 thousand fragments (157917+8765240 sites), 333 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     325 bp
  30% of sequence in fragments shorter than     374 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     478 bp
  45% of sequence in fragments shorter than     534 bp
  50% of sequence in fragments shorter than     593 bp
  55% of sequence in fragments shorter than     658 bp
  60% of sequence in fragments shorter than     727 bp
  65% of sequence in fragments shorter than     806 bp
  70% of sequence in fragments shorter than     897 bp
  75% of sequence in fragments shorter than     997 bp
  80% of sequence in fragments shorter than    1123 bp
  85% of sequence in fragments shorter than    1285 bp
  90% of sequence in fragments shorter than    1505 bp
  95% of sequence in fragments shorter than    1924 bp

# AscI.HinfI (GGCGCGCC+GANTC) double digest, 8920 thousand fragments (4751+8916054 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     359 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     509 bp
  50% of sequence in fragments shorter than     564 bp
  55% of sequence in fragments shorter than     623 bp
  60% of sequence in fragments shorter than     685 bp
  65% of sequence in fragments shorter than     753 bp
  70% of sequence in fragments shorter than     831 bp
  75% of sequence in fragments shorter than     923 bp
  80% of sequence in fragments shorter than    1033 bp
  85% of sequence in fragments shorter than    1168 bp
  90% of sequence in fragments shorter than    1357 bp
  95% of sequence in fragments shorter than    1680 bp

# AvaII.BccI (GGWCC+CCATC) double digest, 8918 thousand fragments (2747690+6170927 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     330 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     434 bp
  40% of sequence in fragments shorter than     488 bp
  45% of sequence in fragments shorter than     550 bp
  50% of sequence in fragments shorter than     616 bp
  55% of sequence in fragments shorter than     687 bp
  60% of sequence in fragments shorter than     763 bp
  65% of sequence in fragments shorter than     851 bp
  70% of sequence in fragments shorter than     951 bp
  75% of sequence in fragments shorter than    1069 bp
  80% of sequence in fragments shorter than    1212 bp
  85% of sequence in fragments shorter than    1398 bp
  90% of sequence in fragments shorter than    1673 bp
  95% of sequence in fragments shorter than    2173 bp

# MlyI.TfiI (GAGTC+GAWTC) double digest, 8915 thousand fragments (3931692+4984362 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     510 bp
  50% of sequence in fragments shorter than     564 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     686 bp
  65% of sequence in fragments shorter than     754 bp
  70% of sequence in fragments shorter than     832 bp
  75% of sequence in fragments shorter than     924 bp
  80% of sequence in fragments shorter than    1033 bp
  85% of sequence in fragments shorter than    1169 bp
  90% of sequence in fragments shorter than    1358 bp
  95% of sequence in fragments shorter than    1681 bp

# HinfI.TfiI (GANTC+GAWTC) double digest, 8915 thousand fragments (8916054+4984362 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     510 bp
  50% of sequence in fragments shorter than     564 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     686 bp
  65% of sequence in fragments shorter than     754 bp
  70% of sequence in fragments shorter than     832 bp
  75% of sequence in fragments shorter than     924 bp
  80% of sequence in fragments shorter than    1033 bp
  85% of sequence in fragments shorter than    1169 bp
  90% of sequence in fragments shorter than    1358 bp
  95% of sequence in fragments shorter than    1681 bp

# HinfI.MlyI (GANTC+GAGTC) double digest, 8915 thousand fragments (8916054+3931692 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     276 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     510 bp
  50% of sequence in fragments shorter than     564 bp
  55% of sequence in fragments shorter than     624 bp
  60% of sequence in fragments shorter than     686 bp
  65% of sequence in fragments shorter than     754 bp
  70% of sequence in fragments shorter than     832 bp
  75% of sequence in fragments shorter than     924 bp
  80% of sequence in fragments shorter than    1033 bp
  85% of sequence in fragments shorter than    1169 bp
  90% of sequence in fragments shorter than    1358 bp
  95% of sequence in fragments shorter than    1681 bp

# BfiI.BspKT6I (ACTGGG+GATC) double digest, 8905 thousand fragments (1706594+7199434 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     213 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     314 bp
  30% of sequence in fragments shorter than     360 bp
  35% of sequence in fragments shorter than     409 bp
  40% of sequence in fragments shorter than     459 bp
  45% of sequence in fragments shorter than     511 bp
  50% of sequence in fragments shorter than     568 bp
  55% of sequence in fragments shorter than     628 bp
  60% of sequence in fragments shorter than     694 bp
  65% of sequence in fragments shorter than     769 bp
  70% of sequence in fragments shorter than     855 bp
  75% of sequence in fragments shorter than     952 bp
  80% of sequence in fragments shorter than    1068 bp
  85% of sequence in fragments shorter than    1217 bp
  90% of sequence in fragments shorter than    1430 bp
  95% of sequence in fragments shorter than    1819 bp

# BccI.SmlI (CCATC+CTYRAG) double digest, 8898 thousand fragments (6170927+2727867 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     224 bp
  20% of sequence in fragments shorter than     275 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     370 bp
  35% of sequence in fragments shorter than     420 bp
  40% of sequence in fragments shorter than     471 bp
  45% of sequence in fragments shorter than     527 bp
  50% of sequence in fragments shorter than     587 bp
  55% of sequence in fragments shorter than     650 bp
  60% of sequence in fragments shorter than     720 bp
  65% of sequence in fragments shorter than     798 bp
  70% of sequence in fragments shorter than     887 bp
  75% of sequence in fragments shorter than     991 bp
  80% of sequence in fragments shorter than    1113 bp
  85% of sequence in fragments shorter than    1275 bp
  90% of sequence in fragments shorter than    1512 bp
  95% of sequence in fragments shorter than    1946 bp

# BstEII.HaeIII (GGTNACC+GGCC) double digest, 8897 thousand fragments (335865+8562230 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than      97 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     235 bp
  20% of sequence in fragments shorter than     300 bp
  25% of sequence in fragments shorter than     352 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     487 bp
  40% of sequence in fragments shorter than     555 bp
  45% of sequence in fragments shorter than     631 bp
  50% of sequence in fragments shorter than     713 bp
  55% of sequence in fragments shorter than     806 bp
  60% of sequence in fragments shorter than     902 bp
  65% of sequence in fragments shorter than    1017 bp
  70% of sequence in fragments shorter than    1147 bp
  75% of sequence in fragments shorter than    1295 bp
  80% of sequence in fragments shorter than    1484 bp
  85% of sequence in fragments shorter than    1728 bp
  90% of sequence in fragments shorter than    2072 bp
  95% of sequence in fragments shorter than    2682 bp

# SnaBI.TspRI (TACGTA+CASTG) double digest, 8897 thousand fragments (132299+8765240 sites), 334 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     183 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     326 bp
  30% of sequence in fragments shorter than     374 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     478 bp
  45% of sequence in fragments shorter than     533 bp
  50% of sequence in fragments shorter than     592 bp
  55% of sequence in fragments shorter than     656 bp
  60% of sequence in fragments shorter than     726 bp
  65% of sequence in fragments shorter than     804 bp
  70% of sequence in fragments shorter than     894 bp
  75% of sequence in fragments shorter than     993 bp
  80% of sequence in fragments shorter than    1117 bp
  85% of sequence in fragments shorter than    1277 bp
  90% of sequence in fragments shorter than    1497 bp
  95% of sequence in fragments shorter than    1897 bp

# NaeI.TspRI (GCCGGC+CASTG) double digest, 8890 thousand fragments (125692+8765240 sites), 335 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     183 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     327 bp
  30% of sequence in fragments shorter than     375 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     480 bp
  45% of sequence in fragments shorter than     536 bp
  50% of sequence in fragments shorter than     596 bp
  55% of sequence in fragments shorter than     660 bp
  60% of sequence in fragments shorter than     730 bp
  65% of sequence in fragments shorter than     810 bp
  70% of sequence in fragments shorter than     901 bp
  75% of sequence in fragments shorter than    1001 bp
  80% of sequence in fragments shorter than    1128 bp
  85% of sequence in fragments shorter than    1291 bp
  90% of sequence in fragments shorter than    1511 bp
  95% of sequence in fragments shorter than    1933 bp

# TspRI.XhoI (CASTG+CTCGAG) double digest, 8886 thousand fragments (8765240+121308 sites), 335 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     183 bp
  15% of sequence in fragments shorter than     228 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     327 bp
  30% of sequence in fragments shorter than     375 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     479 bp
  45% of sequence in fragments shorter than     535 bp
  50% of sequence in fragments shorter than     595 bp
  55% of sequence in fragments shorter than     660 bp
  60% of sequence in fragments shorter than     730 bp
  65% of sequence in fragments shorter than     809 bp
  70% of sequence in fragments shorter than     900 bp
  75% of sequence in fragments shorter than    1000 bp
  80% of sequence in fragments shorter than    1125 bp
  85% of sequence in fragments shorter than    1288 bp
  90% of sequence in fragments shorter than    1511 bp
  95% of sequence in fragments shorter than    1932 bp

# BspNCI.EsaBC3I (CCAGA+TCGA) double digest, 8885 thousand fragments (7282436+1603346 sites), 335 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     278 bp
  25% of sequence in fragments shorter than     326 bp
  30% of sequence in fragments shorter than     373 bp
  35% of sequence in fragments shorter than     423 bp
  40% of sequence in fragments shorter than     475 bp
  45% of sequence in fragments shorter than     529 bp
  50% of sequence in fragments shorter than     586 bp
  55% of sequence in fragments shorter than     648 bp
  60% of sequence in fragments shorter than     716 bp
  65% of sequence in fragments shorter than     792 bp
  70% of sequence in fragments shorter than     880 bp
  75% of sequence in fragments shorter than     983 bp
  80% of sequence in fragments shorter than    1107 bp
  85% of sequence in fragments shorter than    1255 bp
  90% of sequence in fragments shorter than    1476 bp
  95% of sequence in fragments shorter than    1856 bp

# HaeIII.SexAI (GGCC+ACCWGGT) double digest, 8859 thousand fragments (8562230+297900 sites), 336 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     236 bp
  20% of sequence in fragments shorter than     300 bp
  25% of sequence in fragments shorter than     353 bp
  30% of sequence in fragments shorter than     421 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     557 bp
  45% of sequence in fragments shorter than     635 bp
  50% of sequence in fragments shorter than     717 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     908 bp
  65% of sequence in fragments shorter than    1024 bp
  70% of sequence in fragments shorter than    1155 bp
  75% of sequence in fragments shorter than    1304 bp
  80% of sequence in fragments shorter than    1495 bp
  85% of sequence in fragments shorter than    1742 bp
  90% of sequence in fragments shorter than    2093 bp
  95% of sequence in fragments shorter than    2713 bp

# BsrI.StyI (ACTGG+CCWWGG) double digest, 8854 thousand fragments (5741310+3114107 sites), 336 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     275 bp
  25% of sequence in fragments shorter than     325 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     480 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     665 bp
  60% of sequence in fragments shorter than     733 bp
  65% of sequence in fragments shorter than     815 bp
  70% of sequence in fragments shorter than     905 bp
  75% of sequence in fragments shorter than    1014 bp
  80% of sequence in fragments shorter than    1144 bp
  85% of sequence in fragments shorter than    1317 bp
  90% of sequence in fragments shorter than    1553 bp
  95% of sequence in fragments shorter than    1984 bp

# BscAI.FokI (GCATC+GGATG) double digest, 8853 thousand fragments (3652926+5201116 sites), 336 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     316 bp
  25% of sequence in fragments shorter than     371 bp
  30% of sequence in fragments shorter than     429 bp
  35% of sequence in fragments shorter than     490 bp
  40% of sequence in fragments shorter than     551 bp
  45% of sequence in fragments shorter than     615 bp
  50% of sequence in fragments shorter than     684 bp
  55% of sequence in fragments shorter than     756 bp
  60% of sequence in fragments shorter than     832 bp
  65% of sequence in fragments shorter than     921 bp
  70% of sequence in fragments shorter than    1020 bp
  75% of sequence in fragments shorter than    1134 bp
  80% of sequence in fragments shorter than    1277 bp
  85% of sequence in fragments shorter than    1449 bp
  90% of sequence in fragments shorter than    1704 bp
  95% of sequence in fragments shorter than    2147 bp

# ClaI.TspRI (ATCGAT+CASTG) double digest, 8851 thousand fragments (86477+8765240 sites), 336 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     328 bp
  30% of sequence in fragments shorter than     376 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     481 bp
  45% of sequence in fragments shorter than     536 bp
  50% of sequence in fragments shorter than     596 bp
  55% of sequence in fragments shorter than     660 bp
  60% of sequence in fragments shorter than     730 bp
  65% of sequence in fragments shorter than     808 bp
  70% of sequence in fragments shorter than     898 bp
  75% of sequence in fragments shorter than     998 bp
  80% of sequence in fragments shorter than    1123 bp
  85% of sequence in fragments shorter than    1285 bp
  90% of sequence in fragments shorter than    1505 bp
  95% of sequence in fragments shorter than    1923 bp

# HaeIII.KpnI (GGCC+GGTACC) double digest, 8849 thousand fragments (8562230+287974 sites), 336 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     236 bp
  20% of sequence in fragments shorter than     301 bp
  25% of sequence in fragments shorter than     353 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     558 bp
  45% of sequence in fragments shorter than     634 bp
  50% of sequence in fragments shorter than     713 bp
  55% of sequence in fragments shorter than     807 bp
  60% of sequence in fragments shorter than     907 bp
  65% of sequence in fragments shorter than    1024 bp
  70% of sequence in fragments shorter than    1154 bp
  75% of sequence in fragments shorter than    1301 bp
  80% of sequence in fragments shorter than    1492 bp
  85% of sequence in fragments shorter than    1737 bp
  90% of sequence in fragments shorter than    2088 bp
  95% of sequence in fragments shorter than    2710 bp

# HaeIII.NheI (GGCC+GCTAGC) double digest, 8849 thousand fragments (8562230+287384 sites), 336 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     237 bp
  20% of sequence in fragments shorter than     301 bp
  25% of sequence in fragments shorter than     354 bp
  30% of sequence in fragments shorter than     418 bp
  35% of sequence in fragments shorter than     483 bp
  40% of sequence in fragments shorter than     549 bp
  45% of sequence in fragments shorter than     626 bp
  50% of sequence in fragments shorter than     709 bp
  55% of sequence in fragments shorter than     799 bp
  60% of sequence in fragments shorter than     897 bp
  65% of sequence in fragments shorter than    1012 bp
  70% of sequence in fragments shorter than    1137 bp
  75% of sequence in fragments shorter than    1291 bp
  80% of sequence in fragments shorter than    1481 bp
  85% of sequence in fragments shorter than    1729 bp
  90% of sequence in fragments shorter than    2080 bp
  95% of sequence in fragments shorter than    2696 bp

# BspKT6I.PsiI (GATC+TTATAA) double digest, 8846 thousand fragments (7199434+1647912 sites), 336 bp average fragment length
   5% of sequence in fragments shorter than     124 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     266 bp
  25% of sequence in fragments shorter than     313 bp
  30% of sequence in fragments shorter than     358 bp
  35% of sequence in fragments shorter than     407 bp
  40% of sequence in fragments shorter than     456 bp
  45% of sequence in fragments shorter than     505 bp
  50% of sequence in fragments shorter than     561 bp
  55% of sequence in fragments shorter than     619 bp
  60% of sequence in fragments shorter than     683 bp
  65% of sequence in fragments shorter than     754 bp
  70% of sequence in fragments shorter than     836 bp
  75% of sequence in fragments shorter than     930 bp
  80% of sequence in fragments shorter than    1040 bp
  85% of sequence in fragments shorter than    1181 bp
  90% of sequence in fragments shorter than    1384 bp
  95% of sequence in fragments shorter than    1755 bp

# BsrI.NspI (ACTGG+RCATGY) double digest, 8845 thousand fragments (5741310+3104359 sites), 336 bp average fragment length
   5% of sequence in fragments shorter than     116 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     278 bp
  25% of sequence in fragments shorter than     325 bp
  30% of sequence in fragments shorter than     375 bp
  35% of sequence in fragments shorter than     426 bp
  40% of sequence in fragments shorter than     477 bp
  45% of sequence in fragments shorter than     532 bp
  50% of sequence in fragments shorter than     591 bp
  55% of sequence in fragments shorter than     652 bp
  60% of sequence in fragments shorter than     714 bp
  65% of sequence in fragments shorter than     788 bp
  70% of sequence in fragments shorter than     871 bp
  75% of sequence in fragments shorter than     970 bp
  80% of sequence in fragments shorter than    1087 bp
  85% of sequence in fragments shorter than    1237 bp
  90% of sequence in fragments shorter than    1449 bp
  95% of sequence in fragments shorter than    1827 bp

# AatII.TspRI (GACGTC+CASTG) double digest, 8835 thousand fragments (71089+8765240 sites), 337 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     328 bp
  30% of sequence in fragments shorter than     377 bp
  35% of sequence in fragments shorter than     429 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     663 bp
  60% of sequence in fragments shorter than     734 bp
  65% of sequence in fragments shorter than     813 bp
  70% of sequence in fragments shorter than     905 bp
  75% of sequence in fragments shorter than    1005 bp
  80% of sequence in fragments shorter than    1132 bp
  85% of sequence in fragments shorter than    1295 bp
  90% of sequence in fragments shorter than    1515 bp
  95% of sequence in fragments shorter than    1936 bp

# BspNCI.HgiCI (CCAGA+GGYRCC) double digest, 8832 thousand fragments (7282436+1550877 sites), 337 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     230 bp
  20% of sequence in fragments shorter than     283 bp
  25% of sequence in fragments shorter than     335 bp
  30% of sequence in fragments shorter than     385 bp
  35% of sequence in fragments shorter than     438 bp
  40% of sequence in fragments shorter than     492 bp
  45% of sequence in fragments shorter than     550 bp
  50% of sequence in fragments shorter than     608 bp
  55% of sequence in fragments shorter than     672 bp
  60% of sequence in fragments shorter than     744 bp
  65% of sequence in fragments shorter than     827 bp
  70% of sequence in fragments shorter than     919 bp
  75% of sequence in fragments shorter than    1029 bp
  80% of sequence in fragments shorter than    1160 bp
  85% of sequence in fragments shorter than    1330 bp
  90% of sequence in fragments shorter than    1572 bp
  95% of sequence in fragments shorter than    1998 bp

# SacII.TspRI (CCGCGG+CASTG) double digest, 8831 thousand fragments (66901+8765240 sites), 337 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     329 bp
  30% of sequence in fragments shorter than     377 bp
  35% of sequence in fragments shorter than     429 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     663 bp
  60% of sequence in fragments shorter than     734 bp
  65% of sequence in fragments shorter than     813 bp
  70% of sequence in fragments shorter than     905 bp
  75% of sequence in fragments shorter than    1005 bp
  80% of sequence in fragments shorter than    1132 bp
  85% of sequence in fragments shorter than    1295 bp
  90% of sequence in fragments shorter than    1516 bp
  95% of sequence in fragments shorter than    1939 bp

# BsmI.BspNCI (GAATGC+CCAGA) double digest, 8831 thousand fragments (1549350+7282436 sites), 337 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     332 bp
  30% of sequence in fragments shorter than     385 bp
  35% of sequence in fragments shorter than     439 bp
  40% of sequence in fragments shorter than     495 bp
  45% of sequence in fragments shorter than     552 bp
  50% of sequence in fragments shorter than     613 bp
  55% of sequence in fragments shorter than     679 bp
  60% of sequence in fragments shorter than     750 bp
  65% of sequence in fragments shorter than     832 bp
  70% of sequence in fragments shorter than     925 bp
  75% of sequence in fragments shorter than    1027 bp
  80% of sequence in fragments shorter than    1153 bp
  85% of sequence in fragments shorter than    1317 bp
  90% of sequence in fragments shorter than    1548 bp
  95% of sequence in fragments shorter than    1941 bp

# PspXI.TspRI (VCTCGAGB+CASTG) double digest, 8823 thousand fragments (59193+8765240 sites), 337 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     230 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     329 bp
  30% of sequence in fragments shorter than     378 bp
  35% of sequence in fragments shorter than     429 bp
  40% of sequence in fragments shorter than     483 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     600 bp
  55% of sequence in fragments shorter than     665 bp
  60% of sequence in fragments shorter than     735 bp
  65% of sequence in fragments shorter than     815 bp
  70% of sequence in fragments shorter than     907 bp
  75% of sequence in fragments shorter than    1008 bp
  80% of sequence in fragments shorter than    1134 bp
  85% of sequence in fragments shorter than    1298 bp
  90% of sequence in fragments shorter than    1519 bp
  95% of sequence in fragments shorter than    1942 bp

# BbvI.SfeI (GCAGC+CTRYAG) double digest, 8820 thousand fragments (5290051+3531009 sites), 337 bp average fragment length
   5% of sequence in fragments shorter than     124 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     316 bp
  25% of sequence in fragments shorter than     374 bp
  30% of sequence in fragments shorter than     435 bp
  35% of sequence in fragments shorter than     495 bp
  40% of sequence in fragments shorter than     559 bp
  45% of sequence in fragments shorter than     626 bp
  50% of sequence in fragments shorter than     697 bp
  55% of sequence in fragments shorter than     776 bp
  60% of sequence in fragments shorter than     861 bp
  65% of sequence in fragments shorter than     953 bp
  70% of sequence in fragments shorter than    1051 bp
  75% of sequence in fragments shorter than    1171 bp
  80% of sequence in fragments shorter than    1323 bp
  85% of sequence in fragments shorter than    1513 bp
  90% of sequence in fragments shorter than    1782 bp
  95% of sequence in fragments shorter than    2248 bp

# AgeI.TspRI (ACCGGT+CASTG) double digest, 8818 thousand fragments (53386+8765240 sites), 337 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     230 bp
  20% of sequence in fragments shorter than     280 bp
  25% of sequence in fragments shorter than     329 bp
  30% of sequence in fragments shorter than     378 bp
  35% of sequence in fragments shorter than     429 bp
  40% of sequence in fragments shorter than     483 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     600 bp
  55% of sequence in fragments shorter than     664 bp
  60% of sequence in fragments shorter than     735 bp
  65% of sequence in fragments shorter than     814 bp
  70% of sequence in fragments shorter than     906 bp
  75% of sequence in fragments shorter than    1007 bp
  80% of sequence in fragments shorter than    1133 bp
  85% of sequence in fragments shorter than    1296 bp
  90% of sequence in fragments shorter than    1518 bp
  95% of sequence in fragments shorter than    1941 bp

# HhaI.Hin4II (GCGC+CCTTC) double digest, 8815 thousand fragments (1756502+7059915 sites), 338 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     181 bp
  15% of sequence in fragments shorter than     239 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     349 bp
  30% of sequence in fragments shorter than     405 bp
  35% of sequence in fragments shorter than     463 bp
  40% of sequence in fragments shorter than     524 bp
  45% of sequence in fragments shorter than     585 bp
  50% of sequence in fragments shorter than     654 bp
  55% of sequence in fragments shorter than     728 bp
  60% of sequence in fragments shorter than     810 bp
  65% of sequence in fragments shorter than     902 bp
  70% of sequence in fragments shorter than    1008 bp
  75% of sequence in fragments shorter than    1128 bp
  80% of sequence in fragments shorter than    1277 bp
  85% of sequence in fragments shorter than    1467 bp
  90% of sequence in fragments shorter than    1734 bp
  95% of sequence in fragments shorter than    2184 bp

# PmeI.TspRI (GTTTAAAC+CASTG) double digest, 8806 thousand fragments (42094+8765240 sites), 338 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     230 bp
  20% of sequence in fragments shorter than     280 bp
  25% of sequence in fragments shorter than     329 bp
  30% of sequence in fragments shorter than     378 bp
  35% of sequence in fragments shorter than     430 bp
  40% of sequence in fragments shorter than     483 bp
  45% of sequence in fragments shorter than     540 bp
  50% of sequence in fragments shorter than     600 bp
  55% of sequence in fragments shorter than     665 bp
  60% of sequence in fragments shorter than     735 bp
  65% of sequence in fragments shorter than     814 bp
  70% of sequence in fragments shorter than     906 bp
  75% of sequence in fragments shorter than    1006 bp
  80% of sequence in fragments shorter than    1133 bp
  85% of sequence in fragments shorter than    1296 bp
  90% of sequence in fragments shorter than    1516 bp
  95% of sequence in fragments shorter than    1936 bp

# BspKT6I.EsaBC3I (GATC+TCGA) double digest, 8802 thousand fragments (7199434+1603346 sites), 338 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     282 bp
  25% of sequence in fragments shorter than     332 bp
  30% of sequence in fragments shorter than     382 bp
  35% of sequence in fragments shorter than     433 bp
  40% of sequence in fragments shorter than     484 bp
  45% of sequence in fragments shorter than     540 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     662 bp
  60% of sequence in fragments shorter than     730 bp
  65% of sequence in fragments shorter than     808 bp
  70% of sequence in fragments shorter than     894 bp
  75% of sequence in fragments shorter than     995 bp
  80% of sequence in fragments shorter than    1108 bp
  85% of sequence in fragments shorter than    1262 bp
  90% of sequence in fragments shorter than    1475 bp
  95% of sequence in fragments shorter than    1851 bp

# BsrBI.HaeIII (CCGCTC+GGCC) double digest, 8797 thousand fragments (235523+8562230 sites), 338 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     172 bp
  15% of sequence in fragments shorter than     240 bp
  20% of sequence in fragments shorter than     306 bp
  25% of sequence in fragments shorter than     360 bp
  30% of sequence in fragments shorter than     428 bp
  35% of sequence in fragments shorter than     498 bp
  40% of sequence in fragments shorter than     570 bp
  45% of sequence in fragments shorter than     649 bp
  50% of sequence in fragments shorter than     735 bp
  55% of sequence in fragments shorter than     830 bp
  60% of sequence in fragments shorter than     929 bp
  65% of sequence in fragments shorter than    1046 bp
  70% of sequence in fragments shorter than    1184 bp
  75% of sequence in fragments shorter than    1342 bp
  80% of sequence in fragments shorter than    1540 bp
  85% of sequence in fragments shorter than    1796 bp
  90% of sequence in fragments shorter than    2160 bp
  95% of sequence in fragments shorter than    2800 bp

# SalI.TspRI (GTCGAC+CASTG) double digest, 8796 thousand fragments (31396+8765240 sites), 338 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     230 bp
  20% of sequence in fragments shorter than     280 bp
  25% of sequence in fragments shorter than     330 bp
  30% of sequence in fragments shorter than     379 bp
  35% of sequence in fragments shorter than     431 bp
  40% of sequence in fragments shorter than     484 bp
  45% of sequence in fragments shorter than     541 bp
  50% of sequence in fragments shorter than     601 bp
  55% of sequence in fragments shorter than     666 bp
  60% of sequence in fragments shorter than     737 bp
  65% of sequence in fragments shorter than     817 bp
  70% of sequence in fragments shorter than     908 bp
  75% of sequence in fragments shorter than    1010 bp
  80% of sequence in fragments shorter than    1136 bp
  85% of sequence in fragments shorter than    1301 bp
  90% of sequence in fragments shorter than    1522 bp
  95% of sequence in fragments shorter than    1945 bp

# HaeIII.KasI (GGCC+GGCGCC) double digest, 8792 thousand fragments (8562230+230381 sites), 338 bp average fragment length
   5% of sequence in fragments shorter than      98 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     237 bp
  20% of sequence in fragments shorter than     304 bp
  25% of sequence in fragments shorter than     358 bp
  30% of sequence in fragments shorter than     427 bp
  35% of sequence in fragments shorter than     498 bp
  40% of sequence in fragments shorter than     571 bp
  45% of sequence in fragments shorter than     651 bp
  50% of sequence in fragments shorter than     737 bp
  55% of sequence in fragments shorter than     836 bp
  60% of sequence in fragments shorter than     936 bp
  65% of sequence in fragments shorter than    1054 bp
  70% of sequence in fragments shorter than    1193 bp
  75% of sequence in fragments shorter than    1349 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1806 bp
  90% of sequence in fragments shorter than    2173 bp
  95% of sequence in fragments shorter than    2816 bp

# HaeIII.SwaI (GGCC+ATTTAAAT) double digest, 8788 thousand fragments (8562230+226602 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     239 bp
  20% of sequence in fragments shorter than     304 bp
  25% of sequence in fragments shorter than     356 bp
  30% of sequence in fragments shorter than     422 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     556 bp
  45% of sequence in fragments shorter than     632 bp
  50% of sequence in fragments shorter than     712 bp
  55% of sequence in fragments shorter than     803 bp
  60% of sequence in fragments shorter than     896 bp
  65% of sequence in fragments shorter than    1007 bp
  70% of sequence in fragments shorter than    1132 bp
  75% of sequence in fragments shorter than    1274 bp
  80% of sequence in fragments shorter than    1455 bp
  85% of sequence in fragments shorter than    1691 bp
  90% of sequence in fragments shorter than    2016 bp
  95% of sequence in fragments shorter than    2591 bp

# MluI.TspRI (ACGCGT+CASTG) double digest, 8786 thousand fragments (21415+8765240 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     330 bp
  30% of sequence in fragments shorter than     379 bp
  35% of sequence in fragments shorter than     431 bp
  40% of sequence in fragments shorter than     485 bp
  45% of sequence in fragments shorter than     541 bp
  50% of sequence in fragments shorter than     602 bp
  55% of sequence in fragments shorter than     667 bp
  60% of sequence in fragments shorter than     738 bp
  65% of sequence in fragments shorter than     818 bp
  70% of sequence in fragments shorter than     910 bp
  75% of sequence in fragments shorter than    1011 bp
  80% of sequence in fragments shorter than    1138 bp
  85% of sequence in fragments shorter than    1302 bp
  90% of sequence in fragments shorter than    1524 bp
  95% of sequence in fragments shorter than    1948 bp

# SgrAI.TspRI (CRCCGGYG+CASTG) double digest, 8780 thousand fragments (15270+8765240 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     330 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     431 bp
  40% of sequence in fragments shorter than     485 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     602 bp
  55% of sequence in fragments shorter than     667 bp
  60% of sequence in fragments shorter than     738 bp
  65% of sequence in fragments shorter than     818 bp
  70% of sequence in fragments shorter than     910 bp
  75% of sequence in fragments shorter than    1011 bp
  80% of sequence in fragments shorter than    1138 bp
  85% of sequence in fragments shorter than    1302 bp
  90% of sequence in fragments shorter than    1524 bp
  95% of sequence in fragments shorter than    1948 bp

# NruI.TspRI (TCGCGA+CASTG) double digest, 8779 thousand fragments (14872+8765240 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     330 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     431 bp
  40% of sequence in fragments shorter than     485 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     603 bp
  55% of sequence in fragments shorter than     668 bp
  60% of sequence in fragments shorter than     738 bp
  65% of sequence in fragments shorter than     818 bp
  70% of sequence in fragments shorter than     910 bp
  75% of sequence in fragments shorter than    1012 bp
  80% of sequence in fragments shorter than    1139 bp
  85% of sequence in fragments shorter than    1303 bp
  90% of sequence in fragments shorter than    1525 bp
  95% of sequence in fragments shorter than    1949 bp

# PvuI.TspRI (CGATCG+CASTG) double digest, 8778 thousand fragments (14186+8765240 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     431 bp
  40% of sequence in fragments shorter than     485 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     603 bp
  55% of sequence in fragments shorter than     668 bp
  60% of sequence in fragments shorter than     738 bp
  65% of sequence in fragments shorter than     818 bp
  70% of sequence in fragments shorter than     910 bp
  75% of sequence in fragments shorter than    1012 bp
  80% of sequence in fragments shorter than    1138 bp
  85% of sequence in fragments shorter than    1302 bp
  90% of sequence in fragments shorter than    1524 bp
  95% of sequence in fragments shorter than    1941 bp

# FseI.TspRI (GGCCGGCC+CASTG) double digest, 8778 thousand fragments (13662+8765240 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     330 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     431 bp
  40% of sequence in fragments shorter than     485 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     603 bp
  55% of sequence in fragments shorter than     668 bp
  60% of sequence in fragments shorter than     739 bp
  65% of sequence in fragments shorter than     818 bp
  70% of sequence in fragments shorter than     911 bp
  75% of sequence in fragments shorter than    1012 bp
  80% of sequence in fragments shorter than    1139 bp
  85% of sequence in fragments shorter than    1303 bp
  90% of sequence in fragments shorter than    1525 bp
  95% of sequence in fragments shorter than    1949 bp

# NotI.TspRI (GCGGCCGC+CASTG) double digest, 8774 thousand fragments (9795+8765240 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     432 bp
  40% of sequence in fragments shorter than     486 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     603 bp
  55% of sequence in fragments shorter than     668 bp
  60% of sequence in fragments shorter than     738 bp
  65% of sequence in fragments shorter than     818 bp
  70% of sequence in fragments shorter than     910 bp
  75% of sequence in fragments shorter than    1012 bp
  80% of sequence in fragments shorter than    1138 bp
  85% of sequence in fragments shorter than    1303 bp
  90% of sequence in fragments shorter than    1524 bp
  95% of sequence in fragments shorter than    1948 bp

# RsrII.TspRI (CGGWCCG+CASTG) double digest, 8774 thousand fragments (9764+8765240 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     432 bp
  40% of sequence in fragments shorter than     486 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     603 bp
  55% of sequence in fragments shorter than     668 bp
  60% of sequence in fragments shorter than     739 bp
  65% of sequence in fragments shorter than     819 bp
  70% of sequence in fragments shorter than     911 bp
  75% of sequence in fragments shorter than    1012 bp
  80% of sequence in fragments shorter than    1139 bp
  85% of sequence in fragments shorter than    1303 bp
  90% of sequence in fragments shorter than    1525 bp
  95% of sequence in fragments shorter than    1949 bp

# AscI.TspRI (GGCGCGCC+CASTG) double digest, 8769 thousand fragments (4751+8765240 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     380 bp
  35% of sequence in fragments shorter than     432 bp
  40% of sequence in fragments shorter than     486 bp
  45% of sequence in fragments shorter than     542 bp
  50% of sequence in fragments shorter than     603 bp
  55% of sequence in fragments shorter than     668 bp
  60% of sequence in fragments shorter than     739 bp
  65% of sequence in fragments shorter than     819 bp
  70% of sequence in fragments shorter than     911 bp
  75% of sequence in fragments shorter than    1013 bp
  80% of sequence in fragments shorter than    1140 bp
  85% of sequence in fragments shorter than    1304 bp
  90% of sequence in fragments shorter than    1526 bp
  95% of sequence in fragments shorter than    1951 bp

# BscAI.CviQI (GCATC+GTAC) double digest, 8767 thousand fragments (3652926+5115078 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     282 bp
  25% of sequence in fragments shorter than     332 bp
  30% of sequence in fragments shorter than     382 bp
  35% of sequence in fragments shorter than     433 bp
  40% of sequence in fragments shorter than     486 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     597 bp
  55% of sequence in fragments shorter than     657 bp
  60% of sequence in fragments shorter than     722 bp
  65% of sequence in fragments shorter than     796 bp
  70% of sequence in fragments shorter than     878 bp
  75% of sequence in fragments shorter than     976 bp
  80% of sequence in fragments shorter than    1092 bp
  85% of sequence in fragments shorter than    1243 bp
  90% of sequence in fragments shorter than    1439 bp
  95% of sequence in fragments shorter than    1804 bp

# AflIII.BspNCI (ACRYGT+CCAGA) double digest, 8767 thousand fragments (1485394+7282436 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     283 bp
  25% of sequence in fragments shorter than     334 bp
  30% of sequence in fragments shorter than     384 bp
  35% of sequence in fragments shorter than     434 bp
  40% of sequence in fragments shorter than     487 bp
  45% of sequence in fragments shorter than     543 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     663 bp
  60% of sequence in fragments shorter than     731 bp
  65% of sequence in fragments shorter than     810 bp
  70% of sequence in fragments shorter than     896 bp
  75% of sequence in fragments shorter than     999 bp
  80% of sequence in fragments shorter than    1120 bp
  85% of sequence in fragments shorter than    1268 bp
  90% of sequence in fragments shorter than    1487 bp
  95% of sequence in fragments shorter than    1873 bp

# BfiI.Hin4II (ACTGGG+CCTTC) double digest, 8766 thousand fragments (1706594+7059915 sites), 339 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     239 bp
  20% of sequence in fragments shorter than     294 bp
  25% of sequence in fragments shorter than     345 bp
  30% of sequence in fragments shorter than     398 bp
  35% of sequence in fragments shorter than     454 bp
  40% of sequence in fragments shorter than     514 bp
  45% of sequence in fragments shorter than     574 bp
  50% of sequence in fragments shorter than     642 bp
  55% of sequence in fragments shorter than     713 bp
  60% of sequence in fragments shorter than     794 bp
  65% of sequence in fragments shorter than     882 bp
  70% of sequence in fragments shorter than     984 bp
  75% of sequence in fragments shorter than    1100 bp
  80% of sequence in fragments shorter than    1243 bp
  85% of sequence in fragments shorter than    1424 bp
  90% of sequence in fragments shorter than    1682 bp
  95% of sequence in fragments shorter than    2116 bp

# ApoI.SspI (RAATTY+AATATT) double digest, 8759 thousand fragments (6382433+2377267 sites), 340 bp average fragment length
   5% of sequence in fragments shorter than     106 bp
  10% of sequence in fragments shorter than     169 bp
  15% of sequence in fragments shorter than     227 bp
  20% of sequence in fragments shorter than     280 bp
  25% of sequence in fragments shorter than     335 bp
  30% of sequence in fragments shorter than     393 bp
  35% of sequence in fragments shorter than     452 bp
  40% of sequence in fragments shorter than     517 bp
  45% of sequence in fragments shorter than     585 bp
  50% of sequence in fragments shorter than     656 bp
  55% of sequence in fragments shorter than     737 bp
  60% of sequence in fragments shorter than     829 bp
  65% of sequence in fragments shorter than     928 bp
  70% of sequence in fragments shorter than    1050 bp
  75% of sequence in fragments shorter than    1199 bp
  80% of sequence in fragments shorter than    1377 bp
  85% of sequence in fragments shorter than    1624 bp
  90% of sequence in fragments shorter than    2023 bp
  95% of sequence in fragments shorter than    2853 bp

# AvaII.HphI (GGWCC+GGTGA) double digest, 8754 thousand fragments (2747690+6007336 sites), 340 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     226 bp
  20% of sequence in fragments shorter than     279 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     377 bp
  35% of sequence in fragments shorter than     431 bp
  40% of sequence in fragments shorter than     488 bp
  45% of sequence in fragments shorter than     545 bp
  50% of sequence in fragments shorter than     606 bp
  55% of sequence in fragments shorter than     675 bp
  60% of sequence in fragments shorter than     745 bp
  65% of sequence in fragments shorter than     830 bp
  70% of sequence in fragments shorter than     925 bp
  75% of sequence in fragments shorter than    1037 bp
  80% of sequence in fragments shorter than    1176 bp
  85% of sequence in fragments shorter than    1354 bp
  90% of sequence in fragments shorter than    1595 bp
  95% of sequence in fragments shorter than    2032 bp

# BspKT6I.HgiCI (GATC+GGYRCC) double digest, 8749 thousand fragments (7199434+1550877 sites), 340 bp average fragment length
   5% of sequence in fragments shorter than     110 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     219 bp
  20% of sequence in fragments shorter than     272 bp
  25% of sequence in fragments shorter than     322 bp
  30% of sequence in fragments shorter than     371 bp
  35% of sequence in fragments shorter than     423 bp
  40% of sequence in fragments shorter than     474 bp
  45% of sequence in fragments shorter than     530 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     654 bp
  60% of sequence in fragments shorter than     724 bp
  65% of sequence in fragments shorter than     803 bp
  70% of sequence in fragments shorter than     891 bp
  75% of sequence in fragments shorter than     994 bp
  80% of sequence in fragments shorter than    1112 bp
  85% of sequence in fragments shorter than    1271 bp
  90% of sequence in fragments shorter than    1498 bp
  95% of sequence in fragments shorter than    1904 bp

# BsmI.BspKT6I (GAATGC+GATC) double digest, 8748 thousand fragments (1549350+7199434 sites), 340 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     214 bp
  20% of sequence in fragments shorter than     269 bp
  25% of sequence in fragments shorter than     317 bp
  30% of sequence in fragments shorter than     363 bp
  35% of sequence in fragments shorter than     414 bp
  40% of sequence in fragments shorter than     464 bp
  45% of sequence in fragments shorter than     516 bp
  50% of sequence in fragments shorter than     571 bp
  55% of sequence in fragments shorter than     632 bp
  60% of sequence in fragments shorter than     697 bp
  65% of sequence in fragments shorter than     770 bp
  70% of sequence in fragments shorter than     852 bp
  75% of sequence in fragments shorter than     948 bp
  80% of sequence in fragments shorter than    1060 bp
  85% of sequence in fragments shorter than    1206 bp
  90% of sequence in fragments shorter than    1409 bp
  95% of sequence in fragments shorter than    1750 bp

# HphI.SmlI (GGTGA+CTYRAG) double digest, 8734 thousand fragments (6007336+2727867 sites), 341 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     171 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     327 bp
  30% of sequence in fragments shorter than     374 bp
  35% of sequence in fragments shorter than     425 bp
  40% of sequence in fragments shorter than     479 bp
  45% of sequence in fragments shorter than     532 bp
  50% of sequence in fragments shorter than     591 bp
  55% of sequence in fragments shorter than     656 bp
  60% of sequence in fragments shorter than     724 bp
  65% of sequence in fragments shorter than     801 bp
  70% of sequence in fragments shorter than     885 bp
  75% of sequence in fragments shorter than     988 bp
  80% of sequence in fragments shorter than    1111 bp
  85% of sequence in fragments shorter than    1271 bp
  90% of sequence in fragments shorter than    1488 bp
  95% of sequence in fragments shorter than    1892 bp

# AclI.HaeIII (AACGTT+GGCC) double digest, 8734 thousand fragments (172706+8562230 sites), 341 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     241 bp
  20% of sequence in fragments shorter than     305 bp
  25% of sequence in fragments shorter than     359 bp
  30% of sequence in fragments shorter than     426 bp
  35% of sequence in fragments shorter than     493 bp
  40% of sequence in fragments shorter than     563 bp
  45% of sequence in fragments shorter than     641 bp
  50% of sequence in fragments shorter than     724 bp
  55% of sequence in fragments shorter than     818 bp
  60% of sequence in fragments shorter than     916 bp
  65% of sequence in fragments shorter than    1032 bp
  70% of sequence in fragments shorter than    1164 bp
  75% of sequence in fragments shorter than    1312 bp
  80% of sequence in fragments shorter than    1503 bp
  85% of sequence in fragments shorter than    1748 bp
  90% of sequence in fragments shorter than    2098 bp
  95% of sequence in fragments shorter than    2713 bp

# FokI.SfeI (GGATG+CTRYAG) double digest, 8731 thousand fragments (5201116+3531009 sites), 341 bp average fragment length
   5% of sequence in fragments shorter than     116 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     233 bp
  20% of sequence in fragments shorter than     284 bp
  25% of sequence in fragments shorter than     334 bp
  30% of sequence in fragments shorter than     382 bp
  35% of sequence in fragments shorter than     435 bp
  40% of sequence in fragments shorter than     488 bp
  45% of sequence in fragments shorter than     545 bp
  50% of sequence in fragments shorter than     606 bp
  55% of sequence in fragments shorter than     669 bp
  60% of sequence in fragments shorter than     736 bp
  65% of sequence in fragments shorter than     812 bp
  70% of sequence in fragments shorter than     901 bp
  75% of sequence in fragments shorter than    1003 bp
  80% of sequence in fragments shorter than    1121 bp
  85% of sequence in fragments shorter than    1275 bp
  90% of sequence in fragments shorter than    1490 bp
  95% of sequence in fragments shorter than    1874 bp

# BspNCI.FauI (CCAGA+CCCGC) double digest, 8721 thousand fragments (7282436+1439772 sites), 341 bp average fragment length
   5% of sequence in fragments shorter than     111 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     233 bp
  20% of sequence in fragments shorter than     289 bp
  25% of sequence in fragments shorter than     343 bp
  30% of sequence in fragments shorter than     396 bp
  35% of sequence in fragments shorter than     453 bp
  40% of sequence in fragments shorter than     512 bp
  45% of sequence in fragments shorter than     571 bp
  50% of sequence in fragments shorter than     632 bp
  55% of sequence in fragments shorter than     702 bp
  60% of sequence in fragments shorter than     778 bp
  65% of sequence in fragments shorter than     865 bp
  70% of sequence in fragments shorter than     965 bp
  75% of sequence in fragments shorter than    1079 bp
  80% of sequence in fragments shorter than    1212 bp
  85% of sequence in fragments shorter than    1389 bp
  90% of sequence in fragments shorter than    1648 bp
  95% of sequence in fragments shorter than    2092 bp

# HaeIII.PacI (GGCC+TTAATTAA) double digest, 8721 thousand fragments (8562230+159455 sites), 341 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     241 bp
  20% of sequence in fragments shorter than     306 bp
  25% of sequence in fragments shorter than     359 bp
  30% of sequence in fragments shorter than     426 bp
  35% of sequence in fragments shorter than     495 bp
  40% of sequence in fragments shorter than     565 bp
  45% of sequence in fragments shorter than     642 bp
  50% of sequence in fragments shorter than     725 bp
  55% of sequence in fragments shorter than     819 bp
  60% of sequence in fragments shorter than     914 bp
  65% of sequence in fragments shorter than    1030 bp
  70% of sequence in fragments shorter than    1160 bp
  75% of sequence in fragments shorter than    1307 bp
  80% of sequence in fragments shorter than    1495 bp
  85% of sequence in fragments shorter than    1737 bp
  90% of sequence in fragments shorter than    2081 bp
  95% of sequence in fragments shorter than    2687 bp

# BspMI.BspNCI (ACCTGC+CCAGA) double digest, 8720 thousand fragments (1438129+7282436 sites), 341 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     232 bp
  20% of sequence in fragments shorter than     285 bp
  25% of sequence in fragments shorter than     337 bp
  30% of sequence in fragments shorter than     388 bp
  35% of sequence in fragments shorter than     442 bp
  40% of sequence in fragments shorter than     499 bp
  45% of sequence in fragments shorter than     559 bp
  50% of sequence in fragments shorter than     618 bp
  55% of sequence in fragments shorter than     684 bp
  60% of sequence in fragments shorter than     757 bp
  65% of sequence in fragments shorter than     839 bp
  70% of sequence in fragments shorter than     931 bp
  75% of sequence in fragments shorter than    1041 bp
  80% of sequence in fragments shorter than    1165 bp
  85% of sequence in fragments shorter than    1335 bp
  90% of sequence in fragments shorter than    1573 bp
  95% of sequence in fragments shorter than    1988 bp

# HaeIII.Hpy99I (GGCC+CGWCG) double digest, 8719 thousand fragments (8562230+157917 sites), 341 bp average fragment length
   5% of sequence in fragments shorter than      99 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     242 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     362 bp
  30% of sequence in fragments shorter than     430 bp
  35% of sequence in fragments shorter than     501 bp
  40% of sequence in fragments shorter than     574 bp
  45% of sequence in fragments shorter than     654 bp
  50% of sequence in fragments shorter than     739 bp
  55% of sequence in fragments shorter than     837 bp
  60% of sequence in fragments shorter than     936 bp
  65% of sequence in fragments shorter than    1054 bp
  70% of sequence in fragments shorter than    1192 bp
  75% of sequence in fragments shorter than    1348 bp
  80% of sequence in fragments shorter than    1547 bp
  85% of sequence in fragments shorter than    1803 bp
  90% of sequence in fragments shorter than    2168 bp
  95% of sequence in fragments shorter than    2808 bp

# Hin4II.PsiI (CCTTC+TTATAA) double digest, 8707 thousand fragments (7059915+1647912 sites), 342 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     242 bp
  20% of sequence in fragments shorter than     295 bp
  25% of sequence in fragments shorter than     345 bp
  30% of sequence in fragments shorter than     396 bp
  35% of sequence in fragments shorter than     449 bp
  40% of sequence in fragments shorter than     506 bp
  45% of sequence in fragments shorter than     561 bp
  50% of sequence in fragments shorter than     624 bp
  55% of sequence in fragments shorter than     690 bp
  60% of sequence in fragments shorter than     762 bp
  65% of sequence in fragments shorter than     843 bp
  70% of sequence in fragments shorter than     933 bp
  75% of sequence in fragments shorter than    1035 bp
  80% of sequence in fragments shorter than    1162 bp
  85% of sequence in fragments shorter than    1324 bp
  90% of sequence in fragments shorter than    1548 bp
  95% of sequence in fragments shorter than    1924 bp

# ApoI.HpaII (RAATTY+CCGG) double digest, 8699 thousand fragments (6382433+2317722 sites), 342 bp average fragment length
   5% of sequence in fragments shorter than     115 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     231 bp
  20% of sequence in fragments shorter than     281 bp
  25% of sequence in fragments shorter than     333 bp
  30% of sequence in fragments shorter than     384 bp
  35% of sequence in fragments shorter than     437 bp
  40% of sequence in fragments shorter than     493 bp
  45% of sequence in fragments shorter than     552 bp
  50% of sequence in fragments shorter than     614 bp
  55% of sequence in fragments shorter than     680 bp
  60% of sequence in fragments shorter than     750 bp
  65% of sequence in fragments shorter than     832 bp
  70% of sequence in fragments shorter than     919 bp
  75% of sequence in fragments shorter than    1024 bp
  80% of sequence in fragments shorter than    1150 bp
  85% of sequence in fragments shorter than    1308 bp
  90% of sequence in fragments shorter than    1526 bp
  95% of sequence in fragments shorter than    1922 bp

# HaeIII.SnaBI (GGCC+TACGTA) double digest, 8694 thousand fragments (8562230+132299 sites), 342 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     242 bp
  20% of sequence in fragments shorter than     307 bp
  25% of sequence in fragments shorter than     361 bp
  30% of sequence in fragments shorter than     427 bp
  35% of sequence in fragments shorter than     497 bp
  40% of sequence in fragments shorter than     566 bp
  45% of sequence in fragments shorter than     644 bp
  50% of sequence in fragments shorter than     727 bp
  55% of sequence in fragments shorter than     822 bp
  60% of sequence in fragments shorter than     918 bp
  65% of sequence in fragments shorter than    1034 bp
  70% of sequence in fragments shorter than    1168 bp
  75% of sequence in fragments shorter than    1317 bp
  80% of sequence in fragments shorter than    1509 bp
  85% of sequence in fragments shorter than    1755 bp
  90% of sequence in fragments shorter than    2105 bp
  95% of sequence in fragments shorter than    2719 bp

# HaeIII.NaeI (GGCC+GCCGGC) double digest, 8687 thousand fragments (8562230+125692 sites), 343 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     244 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     434 bp
  35% of sequence in fragments shorter than     506 bp
  40% of sequence in fragments shorter than     579 bp
  45% of sequence in fragments shorter than     660 bp
  50% of sequence in fragments shorter than     746 bp
  55% of sequence in fragments shorter than     844 bp
  60% of sequence in fragments shorter than     945 bp
  65% of sequence in fragments shorter than    1063 bp
  70% of sequence in fragments shorter than    1203 bp
  75% of sequence in fragments shorter than    1360 bp
  80% of sequence in fragments shorter than    1559 bp
  85% of sequence in fragments shorter than    1817 bp
  90% of sequence in fragments shorter than    2185 bp
  95% of sequence in fragments shorter than    2832 bp

# AflIII.BspKT6I (ACRYGT+GATC) double digest, 8684 thousand fragments (1485394+7199434 sites), 343 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     221 bp
  20% of sequence in fragments shorter than     274 bp
  25% of sequence in fragments shorter than     323 bp
  30% of sequence in fragments shorter than     370 bp
  35% of sequence in fragments shorter than     418 bp
  40% of sequence in fragments shorter than     467 bp
  45% of sequence in fragments shorter than     517 bp
  50% of sequence in fragments shorter than     574 bp
  55% of sequence in fragments shorter than     635 bp
  60% of sequence in fragments shorter than     701 bp
  65% of sequence in fragments shorter than     775 bp
  70% of sequence in fragments shorter than     860 bp
  75% of sequence in fragments shorter than     957 bp
  80% of sequence in fragments shorter than    1068 bp
  85% of sequence in fragments shorter than    1214 bp
  90% of sequence in fragments shorter than    1421 bp
  95% of sequence in fragments shorter than    1791 bp

# HaeIII.XhoI (GGCC+CTCGAG) double digest, 8683 thousand fragments (8562230+121308 sites), 343 bp average fragment length
   5% of sequence in fragments shorter than     100 bp
  10% of sequence in fragments shorter than     173 bp
  15% of sequence in fragments shorter than     242 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     362 bp
  30% of sequence in fragments shorter than     430 bp
  35% of sequence in fragments shorter than     501 bp
  40% of sequence in fragments shorter than     573 bp
  45% of sequence in fragments shorter than     653 bp
  50% of sequence in fragments shorter than     738 bp
  55% of sequence in fragments shorter than     835 bp
  60% of sequence in fragments shorter than     935 bp
  65% of sequence in fragments shorter than    1053 bp
  70% of sequence in fragments shorter than    1192 bp
  75% of sequence in fragments shorter than    1347 bp
  80% of sequence in fragments shorter than    1545 bp
  85% of sequence in fragments shorter than    1800 bp
  90% of sequence in fragments shorter than    2164 bp
  95% of sequence in fragments shorter than    2804 bp

# EsaBC3I.Hin4II (TCGA+CCTTC) double digest, 8662 thousand fragments (1603346+7059915 sites), 344 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     187 bp
  15% of sequence in fragments shorter than     244 bp
  20% of sequence in fragments shorter than     297 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     400 bp
  35% of sequence in fragments shorter than     453 bp
  40% of sequence in fragments shorter than     510 bp
  45% of sequence in fragments shorter than     567 bp
  50% of sequence in fragments shorter than     633 bp
  55% of sequence in fragments shorter than     701 bp
  60% of sequence in fragments shorter than     779 bp
  65% of sequence in fragments shorter than     864 bp
  70% of sequence in fragments shorter than     960 bp
  75% of sequence in fragments shorter than    1068 bp
  80% of sequence in fragments shorter than    1204 bp
  85% of sequence in fragments shorter than    1379 bp
  90% of sequence in fragments shorter than    1620 bp
  95% of sequence in fragments shorter than    2029 bp

# ClaI.HaeIII (ATCGAT+GGCC) double digest, 8648 thousand fragments (86477+8562230 sites), 344 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     243 bp
  20% of sequence in fragments shorter than     309 bp
  25% of sequence in fragments shorter than     363 bp
  30% of sequence in fragments shorter than     430 bp
  35% of sequence in fragments shorter than     501 bp
  40% of sequence in fragments shorter than     573 bp
  45% of sequence in fragments shorter than     652 bp
  50% of sequence in fragments shorter than     737 bp
  55% of sequence in fragments shorter than     833 bp
  60% of sequence in fragments shorter than     932 bp
  65% of sequence in fragments shorter than    1047 bp
  70% of sequence in fragments shorter than    1184 bp
  75% of sequence in fragments shorter than    1337 bp
  80% of sequence in fragments shorter than    1532 bp
  85% of sequence in fragments shorter than    1784 bp
  90% of sequence in fragments shorter than    2142 bp
  95% of sequence in fragments shorter than    2771 bp

# CviQI.SfeI (GTAC+CTRYAG) double digest, 8645 thousand fragments (5115078+3531009 sites), 344 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     238 bp
  20% of sequence in fragments shorter than     288 bp
  25% of sequence in fragments shorter than     336 bp
  30% of sequence in fragments shorter than     386 bp
  35% of sequence in fragments shorter than     435 bp
  40% of sequence in fragments shorter than     482 bp
  45% of sequence in fragments shorter than     535 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     649 bp
  60% of sequence in fragments shorter than     712 bp
  65% of sequence in fragments shorter than     783 bp
  70% of sequence in fragments shorter than     863 bp
  75% of sequence in fragments shorter than     953 bp
  80% of sequence in fragments shorter than    1066 bp
  85% of sequence in fragments shorter than    1205 bp
  90% of sequence in fragments shorter than    1393 bp
  95% of sequence in fragments shorter than    1741 bp

# BspKT6I.FauI (GATC+CCCGC) double digest, 8638 thousand fragments (7199434+1439772 sites), 344 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     177 bp
  15% of sequence in fragments shorter than     229 bp
  20% of sequence in fragments shorter than     287 bp
  25% of sequence in fragments shorter than     339 bp
  30% of sequence in fragments shorter than     392 bp
  35% of sequence in fragments shorter than     446 bp
  40% of sequence in fragments shorter than     498 bp
  45% of sequence in fragments shorter than     559 bp
  50% of sequence in fragments shorter than     621 bp
  55% of sequence in fragments shorter than     689 bp
  60% of sequence in fragments shorter than     763 bp
  65% of sequence in fragments shorter than     846 bp
  70% of sequence in fragments shorter than     936 bp
  75% of sequence in fragments shorter than    1042 bp
  80% of sequence in fragments shorter than    1172 bp
  85% of sequence in fragments shorter than    1340 bp
  90% of sequence in fragments shorter than    1568 bp
  95% of sequence in fragments shorter than    1985 bp

# BspKT6I.BspMI (GATC+ACCTGC) double digest, 8637 thousand fragments (7199434+1438129 sites), 345 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     223 bp
  20% of sequence in fragments shorter than     277 bp
  25% of sequence in fragments shorter than     325 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     423 bp
  40% of sequence in fragments shorter than     473 bp
  45% of sequence in fragments shorter than     523 bp
  50% of sequence in fragments shorter than     580 bp
  55% of sequence in fragments shorter than     643 bp
  60% of sequence in fragments shorter than     709 bp
  65% of sequence in fragments shorter than     783 bp
  70% of sequence in fragments shorter than     867 bp
  75% of sequence in fragments shorter than     967 bp
  80% of sequence in fragments shorter than    1077 bp
  85% of sequence in fragments shorter than    1229 bp
  90% of sequence in fragments shorter than    1437 bp
  95% of sequence in fragments shorter than    1794 bp

# BscAI.TfiI (GCATC+GAWTC) double digest, 8636 thousand fragments (3652926+4984362 sites), 345 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     180 bp
  15% of sequence in fragments shorter than     232 bp
  20% of sequence in fragments shorter than     283 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     372 bp
  35% of sequence in fragments shorter than     421 bp
  40% of sequence in fragments shorter than     474 bp
  45% of sequence in fragments shorter than     529 bp
  50% of sequence in fragments shorter than     588 bp
  55% of sequence in fragments shorter than     651 bp
  60% of sequence in fragments shorter than     718 bp
  65% of sequence in fragments shorter than     792 bp
  70% of sequence in fragments shorter than     876 bp
  75% of sequence in fragments shorter than     973 bp
  80% of sequence in fragments shorter than    1089 bp
  85% of sequence in fragments shorter than    1236 bp
  90% of sequence in fragments shorter than    1439 bp
  95% of sequence in fragments shorter than    1776 bp

# AatII.HaeIII (GACGTC+GGCC) double digest, 8632 thousand fragments (71089+8562230 sites), 345 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     244 bp
  20% of sequence in fragments shorter than     310 bp
  25% of sequence in fragments shorter than     365 bp
  30% of sequence in fragments shorter than     433 bp
  35% of sequence in fragments shorter than     504 bp
  40% of sequence in fragments shorter than     576 bp
  45% of sequence in fragments shorter than     656 bp
  50% of sequence in fragments shorter than     742 bp
  55% of sequence in fragments shorter than     840 bp
  60% of sequence in fragments shorter than     939 bp
  65% of sequence in fragments shorter than    1057 bp
  70% of sequence in fragments shorter than    1196 bp
  75% of sequence in fragments shorter than    1351 bp
  80% of sequence in fragments shorter than    1549 bp
  85% of sequence in fragments shorter than    1806 bp
  90% of sequence in fragments shorter than    2171 bp
  95% of sequence in fragments shorter than    2812 bp

# HaeIII.SacII (GGCC+CCGCGG) double digest, 8628 thousand fragments (8562230+66901 sites), 345 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     435 bp
  35% of sequence in fragments shorter than     507 bp
  40% of sequence in fragments shorter than     580 bp
  45% of sequence in fragments shorter than     661 bp
  50% of sequence in fragments shorter than     747 bp
  55% of sequence in fragments shorter than     846 bp
  60% of sequence in fragments shorter than     947 bp
  65% of sequence in fragments shorter than    1065 bp
  70% of sequence in fragments shorter than    1205 bp
  75% of sequence in fragments shorter than    1362 bp
  80% of sequence in fragments shorter than    1561 bp
  85% of sequence in fragments shorter than    1820 bp
  90% of sequence in fragments shorter than    2189 bp
  95% of sequence in fragments shorter than    2836 bp

# HaeIII.PspXI (GGCC+VCTCGAGB) double digest, 8620 thousand fragments (8562230+59193 sites), 345 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     244 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     433 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     578 bp
  45% of sequence in fragments shorter than     658 bp
  50% of sequence in fragments shorter than     744 bp
  55% of sequence in fragments shorter than     842 bp
  60% of sequence in fragments shorter than     942 bp
  65% of sequence in fragments shorter than    1060 bp
  70% of sequence in fragments shorter than    1199 bp
  75% of sequence in fragments shorter than    1356 bp
  80% of sequence in fragments shorter than    1554 bp
  85% of sequence in fragments shorter than    1811 bp
  90% of sequence in fragments shorter than    2178 bp
  95% of sequence in fragments shorter than    2821 bp

# AgeI.HaeIII (ACCGGT+GGCC) double digest, 8615 thousand fragments (53386+8562230 sites), 345 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     244 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     433 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     577 bp
  45% of sequence in fragments shorter than     658 bp
  50% of sequence in fragments shorter than     743 bp
  55% of sequence in fragments shorter than     841 bp
  60% of sequence in fragments shorter than     942 bp
  65% of sequence in fragments shorter than    1059 bp
  70% of sequence in fragments shorter than    1198 bp
  75% of sequence in fragments shorter than    1354 bp
  80% of sequence in fragments shorter than    1552 bp
  85% of sequence in fragments shorter than    1809 bp
  90% of sequence in fragments shorter than    2175 bp
  95% of sequence in fragments shorter than    2818 bp

# HgiCI.Hin4II (GGYRCC+CCTTC) double digest, 8610 thousand fragments (1550877+7059915 sites), 346 bp average fragment length
   5% of sequence in fragments shorter than     119 bp
  10% of sequence in fragments shorter than     187 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     299 bp
  25% of sequence in fragments shorter than     349 bp
  30% of sequence in fragments shorter than     404 bp
  35% of sequence in fragments shorter than     462 bp
  40% of sequence in fragments shorter than     522 bp
  45% of sequence in fragments shorter than     585 bp
  50% of sequence in fragments shorter than     653 bp
  55% of sequence in fragments shorter than     727 bp
  60% of sequence in fragments shorter than     809 bp
  65% of sequence in fragments shorter than     899 bp
  70% of sequence in fragments shorter than    1003 bp
  75% of sequence in fragments shorter than    1119 bp
  80% of sequence in fragments shorter than    1265 bp
  85% of sequence in fragments shorter than    1449 bp
  90% of sequence in fragments shorter than    1712 bp
  95% of sequence in fragments shorter than    2150 bp

# BsmI.Hin4II (GAATGC+CCTTC) double digest, 8608 thousand fragments (1549350+7059915 sites), 346 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     240 bp
  20% of sequence in fragments shorter than     298 bp
  25% of sequence in fragments shorter than     352 bp
  30% of sequence in fragments shorter than     409 bp
  35% of sequence in fragments shorter than     468 bp
  40% of sequence in fragments shorter than     528 bp
  45% of sequence in fragments shorter than     591 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     736 bp
  60% of sequence in fragments shorter than     819 bp
  65% of sequence in fragments shorter than     911 bp
  70% of sequence in fragments shorter than    1014 bp
  75% of sequence in fragments shorter than    1134 bp
  80% of sequence in fragments shorter than    1280 bp
  85% of sequence in fragments shorter than    1466 bp
  90% of sequence in fragments shorter than    1725 bp
  95% of sequence in fragments shorter than    2158 bp

# HaeIII.PmeI (GGCC+GTTTAAAC) double digest, 8603 thousand fragments (8562230+42094 sites), 346 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     433 bp
  35% of sequence in fragments shorter than     504 bp
  40% of sequence in fragments shorter than     577 bp
  45% of sequence in fragments shorter than     657 bp
  50% of sequence in fragments shorter than     742 bp
  55% of sequence in fragments shorter than     839 bp
  60% of sequence in fragments shorter than     939 bp
  65% of sequence in fragments shorter than    1056 bp
  70% of sequence in fragments shorter than    1194 bp
  75% of sequence in fragments shorter than    1349 bp
  80% of sequence in fragments shorter than    1545 bp
  85% of sequence in fragments shorter than    1800 bp
  90% of sequence in fragments shorter than    2163 bp
  95% of sequence in fragments shorter than    2800 bp

# BspNCI.PstI (CCAGA+CTGCAG) double digest, 8603 thousand fragments (7282436+1321469 sites), 346 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     234 bp
  20% of sequence in fragments shorter than     288 bp
  25% of sequence in fragments shorter than     342 bp
  30% of sequence in fragments shorter than     396 bp
  35% of sequence in fragments shorter than     451 bp
  40% of sequence in fragments shorter than     509 bp
  45% of sequence in fragments shorter than     568 bp
  50% of sequence in fragments shorter than     629 bp
  55% of sequence in fragments shorter than     697 bp
  60% of sequence in fragments shorter than     772 bp
  65% of sequence in fragments shorter than     859 bp
  70% of sequence in fragments shorter than     955 bp
  75% of sequence in fragments shorter than    1065 bp
  80% of sequence in fragments shorter than    1193 bp
  85% of sequence in fragments shorter than    1369 bp
  90% of sequence in fragments shorter than    1616 bp
  95% of sequence in fragments shorter than    2043 bp

# HaeIII.SalI (GGCC+GTCGAC) double digest, 8593 thousand fragments (8562230+31396 sites), 346 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     434 bp
  35% of sequence in fragments shorter than     506 bp
  40% of sequence in fragments shorter than     579 bp
  45% of sequence in fragments shorter than     659 bp
  50% of sequence in fragments shorter than     745 bp
  55% of sequence in fragments shorter than     843 bp
  60% of sequence in fragments shorter than     944 bp
  65% of sequence in fragments shorter than    1061 bp
  70% of sequence in fragments shorter than    1201 bp
  75% of sequence in fragments shorter than    1357 bp
  80% of sequence in fragments shorter than    1555 bp
  85% of sequence in fragments shorter than    1813 bp
  90% of sequence in fragments shorter than    2179 bp
  95% of sequence in fragments shorter than    2824 bp

# BthCI.SspI (GCNGC+AATATT) double digest, 8586 thousand fragments (6209929+2377267 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     119 bp
  10% of sequence in fragments shorter than     190 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     427 bp
  35% of sequence in fragments shorter than     487 bp
  40% of sequence in fragments shorter than     549 bp
  45% of sequence in fragments shorter than     616 bp
  50% of sequence in fragments shorter than     684 bp
  55% of sequence in fragments shorter than     757 bp
  60% of sequence in fragments shorter than     837 bp
  65% of sequence in fragments shorter than     923 bp
  70% of sequence in fragments shorter than    1025 bp
  75% of sequence in fragments shorter than    1137 bp
  80% of sequence in fragments shorter than    1274 bp
  85% of sequence in fragments shorter than    1453 bp
  90% of sequence in fragments shorter than    1685 bp
  95% of sequence in fragments shorter than    2102 bp

# HaeIII.MluI (GGCC+ACGCGT) double digest, 8583 thousand fragments (8562230+21415 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     435 bp
  35% of sequence in fragments shorter than     507 bp
  40% of sequence in fragments shorter than     580 bp
  45% of sequence in fragments shorter than     661 bp
  50% of sequence in fragments shorter than     747 bp
  55% of sequence in fragments shorter than     845 bp
  60% of sequence in fragments shorter than     946 bp
  65% of sequence in fragments shorter than    1064 bp
  70% of sequence in fragments shorter than    1204 bp
  75% of sequence in fragments shorter than    1360 bp
  80% of sequence in fragments shorter than    1559 bp
  85% of sequence in fragments shorter than    1818 bp
  90% of sequence in fragments shorter than    2186 bp
  95% of sequence in fragments shorter than    2833 bp

# HaeIII.SgrAI (GGCC+CRCCGGYG) double digest, 8577 thousand fragments (8562230+15270 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     581 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     847 bp
  60% of sequence in fragments shorter than     947 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1205 bp
  75% of sequence in fragments shorter than    1362 bp
  80% of sequence in fragments shorter than    1562 bp
  85% of sequence in fragments shorter than    1821 bp
  90% of sequence in fragments shorter than    2189 bp
  95% of sequence in fragments shorter than    2836 bp

# HaeIII.NruI (GGCC+TCGCGA) double digest, 8576 thousand fragments (8562230+14872 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     507 bp
  40% of sequence in fragments shorter than     580 bp
  45% of sequence in fragments shorter than     661 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     846 bp
  60% of sequence in fragments shorter than     947 bp
  65% of sequence in fragments shorter than    1065 bp
  70% of sequence in fragments shorter than    1205 bp
  75% of sequence in fragments shorter than    1362 bp
  80% of sequence in fragments shorter than    1561 bp
  85% of sequence in fragments shorter than    1820 bp
  90% of sequence in fragments shorter than    2189 bp
  95% of sequence in fragments shorter than    2835 bp

# HaeIII.PvuI (GGCC+CGATCG) double digest, 8575 thousand fragments (8562230+14186 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     174 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     507 bp
  40% of sequence in fragments shorter than     580 bp
  45% of sequence in fragments shorter than     661 bp
  50% of sequence in fragments shorter than     747 bp
  55% of sequence in fragments shorter than     846 bp
  60% of sequence in fragments shorter than     947 bp
  65% of sequence in fragments shorter than    1065 bp
  70% of sequence in fragments shorter than    1204 bp
  75% of sequence in fragments shorter than    1362 bp
  80% of sequence in fragments shorter than    1561 bp
  85% of sequence in fragments shorter than    1820 bp
  90% of sequence in fragments shorter than    2186 bp
  95% of sequence in fragments shorter than    2831 bp

# FseI.HaeIII (GGCCGGCC+GGCC) double digest, 8575 thousand fragments (13662+8562230 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     581 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     749 bp
  55% of sequence in fragments shorter than     847 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1206 bp
  75% of sequence in fragments shorter than    1363 bp
  80% of sequence in fragments shorter than    1563 bp
  85% of sequence in fragments shorter than    1822 bp
  90% of sequence in fragments shorter than    2191 bp
  95% of sequence in fragments shorter than    2838 bp

# HaeIII.NotI (GGCC+GCGGCCGC) double digest, 8571 thousand fragments (8562230+9795 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     581 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     749 bp
  55% of sequence in fragments shorter than     847 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1206 bp
  75% of sequence in fragments shorter than    1363 bp
  80% of sequence in fragments shorter than    1563 bp
  85% of sequence in fragments shorter than    1822 bp
  90% of sequence in fragments shorter than    2191 bp
  95% of sequence in fragments shorter than    2838 bp

# HaeIII.RsrII (GGCC+CGGWCCG) double digest, 8571 thousand fragments (8562230+9764 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     101 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     581 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     847 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1206 bp
  75% of sequence in fragments shorter than    1363 bp
  80% of sequence in fragments shorter than    1562 bp
  85% of sequence in fragments shorter than    1821 bp
  90% of sequence in fragments shorter than    2190 bp
  95% of sequence in fragments shorter than    2837 bp

# AscI.HaeIII (GGCGCGCC+GGCC) double digest, 8566 thousand fragments (4751+8562230 sites), 347 bp average fragment length
   5% of sequence in fragments shorter than     102 bp
  10% of sequence in fragments shorter than     175 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     581 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     847 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1206 bp
  75% of sequence in fragments shorter than    1363 bp
  80% of sequence in fragments shorter than    1563 bp
  85% of sequence in fragments shorter than    1822 bp
  90% of sequence in fragments shorter than    2191 bp
  95% of sequence in fragments shorter than    2838 bp

# BccI.SspI (CCATC+AATATT) double digest, 8547 thousand fragments (6170927+2377267 sites), 348 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     237 bp
  20% of sequence in fragments shorter than     287 bp
  25% of sequence in fragments shorter than     335 bp
  30% of sequence in fragments shorter than     384 bp
  35% of sequence in fragments shorter than     433 bp
  40% of sequence in fragments shorter than     484 bp
  45% of sequence in fragments shorter than     538 bp
  50% of sequence in fragments shorter than     595 bp
  55% of sequence in fragments shorter than     655 bp
  60% of sequence in fragments shorter than     721 bp
  65% of sequence in fragments shorter than     794 bp
  70% of sequence in fragments shorter than     877 bp
  75% of sequence in fragments shorter than     974 bp
  80% of sequence in fragments shorter than    1087 bp
  85% of sequence in fragments shorter than    1235 bp
  90% of sequence in fragments shorter than    1439 bp
  95% of sequence in fragments shorter than    1814 bp

# AflIII.Hin4II (ACRYGT+CCTTC) double digest, 8544 thousand fragments (1485394+7059915 sites), 348 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     188 bp
  15% of sequence in fragments shorter than     247 bp
  20% of sequence in fragments shorter than     301 bp
  25% of sequence in fragments shorter than     354 bp
  30% of sequence in fragments shorter than     408 bp
  35% of sequence in fragments shorter than     464 bp
  40% of sequence in fragments shorter than     524 bp
  45% of sequence in fragments shorter than     583 bp
  50% of sequence in fragments shorter than     651 bp
  55% of sequence in fragments shorter than     722 bp
  60% of sequence in fragments shorter than     798 bp
  65% of sequence in fragments shorter than     883 bp
  70% of sequence in fragments shorter than     982 bp
  75% of sequence in fragments shorter than    1092 bp
  80% of sequence in fragments shorter than    1230 bp
  85% of sequence in fragments shorter than    1403 bp
  90% of sequence in fragments shorter than    1648 bp
  95% of sequence in fragments shorter than    2057 bp

# BthCI.HpaII (GCNGC+CCGG) double digest, 8527 thousand fragments (6209929+2317722 sites), 349 bp average fragment length
   5% of sequence in fragments shorter than     113 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     339 bp
  25% of sequence in fragments shorter than     414 bp
  30% of sequence in fragments shorter than     494 bp
  35% of sequence in fragments shorter than     576 bp
  40% of sequence in fragments shorter than     663 bp
  45% of sequence in fragments shorter than     757 bp
  50% of sequence in fragments shorter than     856 bp
  55% of sequence in fragments shorter than     963 bp
  60% of sequence in fragments shorter than    1082 bp
  65% of sequence in fragments shorter than    1219 bp
  70% of sequence in fragments shorter than    1375 bp
  75% of sequence in fragments shorter than    1560 bp
  80% of sequence in fragments shorter than    1773 bp
  85% of sequence in fragments shorter than    2059 bp
  90% of sequence in fragments shorter than    2467 bp
  95% of sequence in fragments shorter than    3169 bp

# BspKT6I.PstI (GATC+CTGCAG) double digest, 8520 thousand fragments (7199434+1321469 sites), 349 bp average fragment length
   5% of sequence in fragments shorter than     129 bp
  10% of sequence in fragments shorter than     177 bp
  15% of sequence in fragments shorter than     225 bp
  20% of sequence in fragments shorter than     280 bp
  25% of sequence in fragments shorter than     331 bp
  30% of sequence in fragments shorter than     381 bp
  35% of sequence in fragments shorter than     433 bp
  40% of sequence in fragments shorter than     484 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     599 bp
  55% of sequence in fragments shorter than     663 bp
  60% of sequence in fragments shorter than     731 bp
  65% of sequence in fragments shorter than     809 bp
  70% of sequence in fragments shorter than     897 bp
  75% of sequence in fragments shorter than    1001 bp
  80% of sequence in fragments shorter than    1116 bp
  85% of sequence in fragments shorter than    1273 bp
  90% of sequence in fragments shorter than    1489 bp
  95% of sequence in fragments shorter than    1867 bp

# SfeI.TfiI (CTRYAG+GAWTC) double digest, 8514 thousand fragments (3531009+4984362 sites), 349 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     239 bp
  20% of sequence in fragments shorter than     290 bp
  25% of sequence in fragments shorter than     335 bp
  30% of sequence in fragments shorter than     376 bp
  35% of sequence in fragments shorter than     427 bp
  40% of sequence in fragments shorter than     479 bp
  45% of sequence in fragments shorter than     532 bp
  50% of sequence in fragments shorter than     590 bp
  55% of sequence in fragments shorter than     652 bp
  60% of sequence in fragments shorter than     713 bp
  65% of sequence in fragments shorter than     786 bp
  70% of sequence in fragments shorter than     864 bp
  75% of sequence in fragments shorter than     957 bp
  80% of sequence in fragments shorter than    1068 bp
  85% of sequence in fragments shorter than    1211 bp
  90% of sequence in fragments shorter than    1407 bp
  95% of sequence in fragments shorter than    1738 bp

# BscGI.BspNCI (CCCGT+CCAGA) double digest, 8513 thousand fragments (1231070+7282436 sites), 350 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     179 bp
  15% of sequence in fragments shorter than     235 bp
  20% of sequence in fragments shorter than     290 bp
  25% of sequence in fragments shorter than     343 bp
  30% of sequence in fragments shorter than     390 bp
  35% of sequence in fragments shorter than     446 bp
  40% of sequence in fragments shorter than     503 bp
  45% of sequence in fragments shorter than     562 bp
  50% of sequence in fragments shorter than     621 bp
  55% of sequence in fragments shorter than     690 bp
  60% of sequence in fragments shorter than     764 bp
  65% of sequence in fragments shorter than     850 bp
  70% of sequence in fragments shorter than     947 bp
  75% of sequence in fragments shorter than    1060 bp
  80% of sequence in fragments shorter than    1187 bp
  85% of sequence in fragments shorter than    1361 bp
  90% of sequence in fragments shorter than    1605 bp
  95% of sequence in fragments shorter than    2015 bp

# FauI.Hin4II (CCCGC+CCTTC) double digest, 8499 thousand fragments (1439772+7059915 sites), 350 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     189 bp
  15% of sequence in fragments shorter than     250 bp
  20% of sequence in fragments shorter than     309 bp
  25% of sequence in fragments shorter than     364 bp
  30% of sequence in fragments shorter than     422 bp
  35% of sequence in fragments shorter than     485 bp
  40% of sequence in fragments shorter than     546 bp
  45% of sequence in fragments shorter than     614 bp
  50% of sequence in fragments shorter than     687 bp
  55% of sequence in fragments shorter than     766 bp
  60% of sequence in fragments shorter than     852 bp
  65% of sequence in fragments shorter than     947 bp
  70% of sequence in fragments shorter than    1053 bp
  75% of sequence in fragments shorter than    1182 bp
  80% of sequence in fragments shorter than    1338 bp
  85% of sequence in fragments shorter than    1535 bp
  90% of sequence in fragments shorter than    1813 bp
  95% of sequence in fragments shorter than    2287 bp

# BspMI.Hin4II (ACCTGC+CCTTC) double digest, 8497 thousand fragments (1438129+7059915 sites), 350 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     187 bp
  15% of sequence in fragments shorter than     247 bp
  20% of sequence in fragments shorter than     303 bp
  25% of sequence in fragments shorter than     355 bp
  30% of sequence in fragments shorter than     413 bp
  35% of sequence in fragments shorter than     471 bp
  40% of sequence in fragments shorter than     529 bp
  45% of sequence in fragments shorter than     594 bp
  50% of sequence in fragments shorter than     664 bp
  55% of sequence in fragments shorter than     740 bp
  60% of sequence in fragments shorter than     822 bp
  65% of sequence in fragments shorter than     914 bp
  70% of sequence in fragments shorter than    1018 bp
  75% of sequence in fragments shorter than    1140 bp
  80% of sequence in fragments shorter than    1286 bp
  85% of sequence in fragments shorter than    1475 bp
  90% of sequence in fragments shorter than    1740 bp
  95% of sequence in fragments shorter than    2188 bp

# AvaII.BsrI (GGWCC+ACTGG) double digest, 8488 thousand fragments (2747690+5741310 sites), 351 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     238 bp
  20% of sequence in fragments shorter than     295 bp
  25% of sequence in fragments shorter than     346 bp
  30% of sequence in fragments shorter than     402 bp
  35% of sequence in fragments shorter than     458 bp
  40% of sequence in fragments shorter than     519 bp
  45% of sequence in fragments shorter than     583 bp
  50% of sequence in fragments shorter than     650 bp
  55% of sequence in fragments shorter than     718 bp
  60% of sequence in fragments shorter than     800 bp
  65% of sequence in fragments shorter than     888 bp
  70% of sequence in fragments shorter than     991 bp
  75% of sequence in fragments shorter than    1109 bp
  80% of sequence in fragments shorter than    1254 bp
  85% of sequence in fragments shorter than    1439 bp
  90% of sequence in fragments shorter than    1708 bp
  95% of sequence in fragments shorter than    2170 bp

# BccI.HpaII (CCATC+CCGG) double digest, 8488 thousand fragments (6170927+2317722 sites), 351 bp average fragment length
   5% of sequence in fragments shorter than     112 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     239 bp
  20% of sequence in fragments shorter than     298 bp
  25% of sequence in fragments shorter than     356 bp
  30% of sequence in fragments shorter than     416 bp
  35% of sequence in fragments shorter than     478 bp
  40% of sequence in fragments shorter than     545 bp
  45% of sequence in fragments shorter than     616 bp
  50% of sequence in fragments shorter than     689 bp
  55% of sequence in fragments shorter than     771 bp
  60% of sequence in fragments shorter than     862 bp
  65% of sequence in fragments shorter than     965 bp
  70% of sequence in fragments shorter than    1077 bp
  75% of sequence in fragments shorter than    1213 bp
  80% of sequence in fragments shorter than    1382 bp
  85% of sequence in fragments shorter than    1605 bp
  90% of sequence in fragments shorter than    1927 bp
  95% of sequence in fragments shorter than    2511 bp

# BsrI.SmlI (ACTGG+CTYRAG) double digest, 8468 thousand fragments (5741310+2727867 sites), 351 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     180 bp
  15% of sequence in fragments shorter than     234 bp
  20% of sequence in fragments shorter than     287 bp
  25% of sequence in fragments shorter than     337 bp
  30% of sequence in fragments shorter than     388 bp
  35% of sequence in fragments shorter than     440 bp
  40% of sequence in fragments shorter than     492 bp
  45% of sequence in fragments shorter than     547 bp
  50% of sequence in fragments shorter than     608 bp
  55% of sequence in fragments shorter than     671 bp
  60% of sequence in fragments shorter than     737 bp
  65% of sequence in fragments shorter than     815 bp
  70% of sequence in fragments shorter than     901 bp
  75% of sequence in fragments shorter than    1005 bp
  80% of sequence in fragments shorter than    1129 bp
  85% of sequence in fragments shorter than    1288 bp
  90% of sequence in fragments shorter than    1513 bp
  95% of sequence in fragments shorter than    1916 bp

# BscGI.BspKT6I (CCCGT+GATC) double digest, 8430 thousand fragments (1231070+7199434 sites), 353 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     177 bp
  15% of sequence in fragments shorter than     233 bp
  20% of sequence in fragments shorter than     288 bp
  25% of sequence in fragments shorter than     339 bp
  30% of sequence in fragments shorter than     384 bp
  35% of sequence in fragments shorter than     437 bp
  40% of sequence in fragments shorter than     489 bp
  45% of sequence in fragments shorter than     546 bp
  50% of sequence in fragments shorter than     607 bp
  55% of sequence in fragments shorter than     672 bp
  60% of sequence in fragments shorter than     742 bp
  65% of sequence in fragments shorter than     822 bp
  70% of sequence in fragments shorter than     911 bp
  75% of sequence in fragments shorter than    1016 bp
  80% of sequence in fragments shorter than    1135 bp
  85% of sequence in fragments shorter than    1292 bp
  90% of sequence in fragments shorter than    1511 bp
  95% of sequence in fragments shorter than    1880 bp

# BbvI.StyI (GCAGC+CCWWGG) double digest, 8403 thousand fragments (5290051+3114107 sites), 354 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     310 bp
  25% of sequence in fragments shorter than     371 bp
  30% of sequence in fragments shorter than     435 bp
  35% of sequence in fragments shorter than     501 bp
  40% of sequence in fragments shorter than     574 bp
  45% of sequence in fragments shorter than     648 bp
  50% of sequence in fragments shorter than     725 bp
  55% of sequence in fragments shorter than     814 bp
  60% of sequence in fragments shorter than     908 bp
  65% of sequence in fragments shorter than    1020 bp
  70% of sequence in fragments shorter than    1145 bp
  75% of sequence in fragments shorter than    1292 bp
  80% of sequence in fragments shorter than    1471 bp
  85% of sequence in fragments shorter than    1709 bp
  90% of sequence in fragments shorter than    2042 bp
  95% of sequence in fragments shorter than    2606 bp

# BbvI.NspI (GCAGC+RCATGY) double digest, 8393 thousand fragments (5290051+3104359 sites), 355 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     191 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     369 bp
  30% of sequence in fragments shorter than     430 bp
  35% of sequence in fragments shorter than     494 bp
  40% of sequence in fragments shorter than     559 bp
  45% of sequence in fragments shorter than     628 bp
  50% of sequence in fragments shorter than     701 bp
  55% of sequence in fragments shorter than     781 bp
  60% of sequence in fragments shorter than     864 bp
  65% of sequence in fragments shorter than     960 bp
  70% of sequence in fragments shorter than    1069 bp
  75% of sequence in fragments shorter than    1200 bp
  80% of sequence in fragments shorter than    1358 bp
  85% of sequence in fragments shorter than    1558 bp
  90% of sequence in fragments shorter than    1818 bp
  95% of sequence in fragments shorter than    2304 bp

# BsmAI.HhaI (GTCTC+GCGC) double digest, 8387 thousand fragments (6631585+1756502 sites), 355 bp average fragment length
   5% of sequence in fragments shorter than     114 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     243 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     377 bp
  30% of sequence in fragments shorter than     446 bp
  35% of sequence in fragments shorter than     517 bp
  40% of sequence in fragments shorter than     587 bp
  45% of sequence in fragments shorter than     664 bp
  50% of sequence in fragments shorter than     743 bp
  55% of sequence in fragments shorter than     836 bp
  60% of sequence in fragments shorter than     936 bp
  65% of sequence in fragments shorter than    1048 bp
  70% of sequence in fragments shorter than    1178 bp
  75% of sequence in fragments shorter than    1332 bp
  80% of sequence in fragments shorter than    1517 bp
  85% of sequence in fragments shorter than    1756 bp
  90% of sequence in fragments shorter than    2078 bp
  95% of sequence in fragments shorter than    2642 bp

# HphI.SspI (GGTGA+AATATT) double digest, 8384 thousand fragments (6007336+2377267 sites), 355 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     237 bp
  20% of sequence in fragments shorter than     288 bp
  25% of sequence in fragments shorter than     337 bp
  30% of sequence in fragments shorter than     384 bp
  35% of sequence in fragments shorter than     434 bp
  40% of sequence in fragments shorter than     485 bp
  45% of sequence in fragments shorter than     539 bp
  50% of sequence in fragments shorter than     595 bp
  55% of sequence in fragments shorter than     657 bp
  60% of sequence in fragments shorter than     722 bp
  65% of sequence in fragments shorter than     795 bp
  70% of sequence in fragments shorter than     874 bp
  75% of sequence in fragments shorter than     972 bp
  80% of sequence in fragments shorter than    1088 bp
  85% of sequence in fragments shorter than    1233 bp
  90% of sequence in fragments shorter than    1434 bp
  95% of sequence in fragments shorter than    1798 bp

# AccI.BspNCI (GTMKAC+CCAGA) double digest, 8383 thousand fragments (1101063+7282436 sites), 355 bp average fragment length
   5% of sequence in fragments shorter than     119 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     241 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     401 bp
  35% of sequence in fragments shorter than     456 bp
  40% of sequence in fragments shorter than     511 bp
  45% of sequence in fragments shorter than     569 bp
  50% of sequence in fragments shorter than     627 bp
  55% of sequence in fragments shorter than     694 bp
  60% of sequence in fragments shorter than     766 bp
  65% of sequence in fragments shorter than     846 bp
  70% of sequence in fragments shorter than     935 bp
  75% of sequence in fragments shorter than    1042 bp
  80% of sequence in fragments shorter than    1161 bp
  85% of sequence in fragments shorter than    1324 bp
  90% of sequence in fragments shorter than    1553 bp
  95% of sequence in fragments shorter than    1956 bp

# Hin4II.PstI (CCTTC+CTGCAG) double digest, 8380 thousand fragments (7059915+1321469 sites), 355 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     191 bp
  15% of sequence in fragments shorter than     250 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     363 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     482 bp
  40% of sequence in fragments shorter than     541 bp
  45% of sequence in fragments shorter than     608 bp
  50% of sequence in fragments shorter than     679 bp
  55% of sequence in fragments shorter than     756 bp
  60% of sequence in fragments shorter than     841 bp
  65% of sequence in fragments shorter than     934 bp
  70% of sequence in fragments shorter than    1040 bp
  75% of sequence in fragments shorter than    1167 bp
  80% of sequence in fragments shorter than    1320 bp
  85% of sequence in fragments shorter than    1513 bp
  90% of sequence in fragments shorter than    1784 bp
  95% of sequence in fragments shorter than    2250 bp

# BspNCI.PpuMI (CCAGA+RGGWCCY) double digest, 8367 thousand fragments (7282436+1085138 sites), 356 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     242 bp
  20% of sequence in fragments shorter than     300 bp
  25% of sequence in fragments shorter than     351 bp
  30% of sequence in fragments shorter than     409 bp
  35% of sequence in fragments shorter than     468 bp
  40% of sequence in fragments shorter than     528 bp
  45% of sequence in fragments shorter than     589 bp
  50% of sequence in fragments shorter than     655 bp
  55% of sequence in fragments shorter than     727 bp
  60% of sequence in fragments shorter than     808 bp
  65% of sequence in fragments shorter than     890 bp
  70% of sequence in fragments shorter than     993 bp
  75% of sequence in fragments shorter than    1111 bp
  80% of sequence in fragments shorter than    1253 bp
  85% of sequence in fragments shorter than    1437 bp
  90% of sequence in fragments shorter than    1703 bp
  95% of sequence in fragments shorter than    2165 bp

# BspNCI.PvuII (CCAGA+CAGCTG) double digest, 8366 thousand fragments (7282436+1084260 sites), 356 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     241 bp
  20% of sequence in fragments shorter than     298 bp
  25% of sequence in fragments shorter than     350 bp
  30% of sequence in fragments shorter than     408 bp
  35% of sequence in fragments shorter than     465 bp
  40% of sequence in fragments shorter than     524 bp
  45% of sequence in fragments shorter than     585 bp
  50% of sequence in fragments shorter than     649 bp
  55% of sequence in fragments shorter than     721 bp
  60% of sequence in fragments shorter than     799 bp
  65% of sequence in fragments shorter than     885 bp
  70% of sequence in fragments shorter than     986 bp
  75% of sequence in fragments shorter than    1102 bp
  80% of sequence in fragments shorter than    1234 bp
  85% of sequence in fragments shorter than    1411 bp
  90% of sequence in fragments shorter than    1667 bp
  95% of sequence in fragments shorter than    2107 bp

# BfiI.BsmAI (ACTGGG+GTCTC) double digest, 8337 thousand fragments (1706594+6631585 sites), 357 bp average fragment length
   5% of sequence in fragments shorter than     124 bp
  10% of sequence in fragments shorter than     179 bp
  15% of sequence in fragments shorter than     235 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     355 bp
  30% of sequence in fragments shorter than     416 bp
  35% of sequence in fragments shorter than     478 bp
  40% of sequence in fragments shorter than     541 bp
  45% of sequence in fragments shorter than     608 bp
  50% of sequence in fragments shorter than     680 bp
  55% of sequence in fragments shorter than     759 bp
  60% of sequence in fragments shorter than     847 bp
  65% of sequence in fragments shorter than     946 bp
  70% of sequence in fragments shorter than    1056 bp
  75% of sequence in fragments shorter than    1188 bp
  80% of sequence in fragments shorter than    1348 bp
  85% of sequence in fragments shorter than    1537 bp
  90% of sequence in fragments shorter than    1832 bp
  95% of sequence in fragments shorter than    2356 bp

# HpaII.HphI (CCGG+GGTGA) double digest, 8324 thousand fragments (2317722+6007336 sites), 357 bp average fragment length
   5% of sequence in fragments shorter than     109 bp
  10% of sequence in fragments shorter than     177 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     431 bp
  35% of sequence in fragments shorter than     496 bp
  40% of sequence in fragments shorter than     563 bp
  45% of sequence in fragments shorter than     634 bp
  50% of sequence in fragments shorter than     710 bp
  55% of sequence in fragments shorter than     794 bp
  60% of sequence in fragments shorter than     882 bp
  65% of sequence in fragments shorter than     989 bp
  70% of sequence in fragments shorter than    1108 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1403 bp
  85% of sequence in fragments shorter than    1619 bp
  90% of sequence in fragments shorter than    1930 bp
  95% of sequence in fragments shorter than    2459 bp

# FokI.StyI (GGATG+CCWWGG) double digest, 8314 thousand fragments (5201116+3114107 sites), 358 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     188 bp
  15% of sequence in fragments shorter than     249 bp
  20% of sequence in fragments shorter than     304 bp
  25% of sequence in fragments shorter than     358 bp
  30% of sequence in fragments shorter than     415 bp
  35% of sequence in fragments shorter than     469 bp
  40% of sequence in fragments shorter than     529 bp
  45% of sequence in fragments shorter than     593 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     734 bp
  60% of sequence in fragments shorter than     814 bp
  65% of sequence in fragments shorter than     904 bp
  70% of sequence in fragments shorter than    1005 bp
  75% of sequence in fragments shorter than    1124 bp
  80% of sequence in fragments shorter than    1268 bp
  85% of sequence in fragments shorter than    1450 bp
  90% of sequence in fragments shorter than    1717 bp
  95% of sequence in fragments shorter than    2187 bp

# FokI.NspI (GGATG+RCATGY) double digest, 8304 thousand fragments (5201116+3104359 sites), 358 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     188 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     299 bp
  25% of sequence in fragments shorter than     352 bp
  30% of sequence in fragments shorter than     405 bp
  35% of sequence in fragments shorter than     460 bp
  40% of sequence in fragments shorter than     516 bp
  45% of sequence in fragments shorter than     576 bp
  50% of sequence in fragments shorter than     639 bp
  55% of sequence in fragments shorter than     707 bp
  60% of sequence in fragments shorter than     781 bp
  65% of sequence in fragments shorter than     863 bp
  70% of sequence in fragments shorter than     953 bp
  75% of sequence in fragments shorter than    1059 bp
  80% of sequence in fragments shorter than    1184 bp
  85% of sequence in fragments shorter than    1348 bp
  90% of sequence in fragments shorter than    1565 bp
  95% of sequence in fragments shorter than    1964 bp

# AccI.BspKT6I (GTMKAC+GATC) double digest, 8299 thousand fragments (1101063+7199434 sites), 359 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     232 bp
  20% of sequence in fragments shorter than     286 bp
  25% of sequence in fragments shorter than     335 bp
  30% of sequence in fragments shorter than     385 bp
  35% of sequence in fragments shorter than     435 bp
  40% of sequence in fragments shorter than     484 bp
  45% of sequence in fragments shorter than     537 bp
  50% of sequence in fragments shorter than     596 bp
  55% of sequence in fragments shorter than     658 bp
  60% of sequence in fragments shorter than     727 bp
  65% of sequence in fragments shorter than     803 bp
  70% of sequence in fragments shorter than     889 bp
  75% of sequence in fragments shorter than     990 bp
  80% of sequence in fragments shorter than    1103 bp
  85% of sequence in fragments shorter than    1254 bp
  90% of sequence in fragments shorter than    1472 bp
  95% of sequence in fragments shorter than    1854 bp

# BscGI.Hin4II (CCCGT+CCTTC) double digest, 8290 thousand fragments (1231070+7059915 sites), 359 bp average fragment length
   5% of sequence in fragments shorter than     124 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     362 bp
  30% of sequence in fragments shorter than     419 bp
  35% of sequence in fragments shorter than     482 bp
  40% of sequence in fragments shorter than     541 bp
  45% of sequence in fragments shorter than     608 bp
  50% of sequence in fragments shorter than     679 bp
  55% of sequence in fragments shorter than     757 bp
  60% of sequence in fragments shorter than     842 bp
  65% of sequence in fragments shorter than     937 bp
  70% of sequence in fragments shorter than    1042 bp
  75% of sequence in fragments shorter than    1169 bp
  80% of sequence in fragments shorter than    1322 bp
  85% of sequence in fragments shorter than    1518 bp
  90% of sequence in fragments shorter than    1792 bp
  95% of sequence in fragments shorter than    2253 bp

# AvaI.BspNCI (CYCGRG+CCAGA) double digest, 8287 thousand fragments (1005714+7282436 sites), 359 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     244 bp
  20% of sequence in fragments shorter than     299 bp
  25% of sequence in fragments shorter than     350 bp
  30% of sequence in fragments shorter than     405 bp
  35% of sequence in fragments shorter than     461 bp
  40% of sequence in fragments shorter than     519 bp
  45% of sequence in fragments shorter than     579 bp
  50% of sequence in fragments shorter than     641 bp
  55% of sequence in fragments shorter than     711 bp
  60% of sequence in fragments shorter than     788 bp
  65% of sequence in fragments shorter than     874 bp
  70% of sequence in fragments shorter than     975 bp
  75% of sequence in fragments shorter than    1089 bp
  80% of sequence in fragments shorter than    1220 bp
  85% of sequence in fragments shorter than    1398 bp
  90% of sequence in fragments shorter than    1656 bp
  95% of sequence in fragments shorter than    2104 bp

# BspKT6I.PpuMI (GATC+RGGWCCY) double digest, 8284 thousand fragments (7199434+1085138 sites), 359 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     232 bp
  20% of sequence in fragments shorter than     287 bp
  25% of sequence in fragments shorter than     337 bp
  30% of sequence in fragments shorter than     388 bp
  35% of sequence in fragments shorter than     438 bp
  40% of sequence in fragments shorter than     489 bp
  45% of sequence in fragments shorter than     546 bp
  50% of sequence in fragments shorter than     606 bp
  55% of sequence in fragments shorter than     668 bp
  60% of sequence in fragments shorter than     739 bp
  65% of sequence in fragments shorter than     819 bp
  70% of sequence in fragments shorter than     907 bp
  75% of sequence in fragments shorter than    1012 bp
  80% of sequence in fragments shorter than    1136 bp
  85% of sequence in fragments shorter than    1299 bp
  90% of sequence in fragments shorter than    1528 bp
  95% of sequence in fragments shorter than    1940 bp

# BspKT6I.PvuII (GATC+CAGCTG) double digest, 8283 thousand fragments (7199434+1084260 sites), 359 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     233 bp
  20% of sequence in fragments shorter than     288 bp
  25% of sequence in fragments shorter than     338 bp
  30% of sequence in fragments shorter than     389 bp
  35% of sequence in fragments shorter than     441 bp
  40% of sequence in fragments shorter than     492 bp
  45% of sequence in fragments shorter than     550 bp
  50% of sequence in fragments shorter than     610 bp
  55% of sequence in fragments shorter than     675 bp
  60% of sequence in fragments shorter than     746 bp
  65% of sequence in fragments shorter than     827 bp
  70% of sequence in fragments shorter than     915 bp
  75% of sequence in fragments shorter than    1019 bp
  80% of sequence in fragments shorter than    1139 bp
  85% of sequence in fragments shorter than    1299 bp
  90% of sequence in fragments shorter than    1522 bp
  95% of sequence in fragments shorter than    1918 bp

# BsmAI.PsiI (GTCTC+TTATAA) double digest, 8279 thousand fragments (6631585+1647912 sites), 359 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     181 bp
  15% of sequence in fragments shorter than     236 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     353 bp
  30% of sequence in fragments shorter than     409 bp
  35% of sequence in fragments shorter than     467 bp
  40% of sequence in fragments shorter than     525 bp
  45% of sequence in fragments shorter than     586 bp
  50% of sequence in fragments shorter than     651 bp
  55% of sequence in fragments shorter than     722 bp
  60% of sequence in fragments shorter than     801 bp
  65% of sequence in fragments shorter than     888 bp
  70% of sequence in fragments shorter than     988 bp
  75% of sequence in fragments shorter than    1103 bp
  80% of sequence in fragments shorter than    1245 bp
  85% of sequence in fragments shorter than    1420 bp
  90% of sequence in fragments shorter than    1677 bp
  95% of sequence in fragments shorter than    2153 bp

# BspHI.BspNCI (TCATGA+CCAGA) double digest, 8260 thousand fragments (978290+7282436 sites), 360 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     244 bp
  20% of sequence in fragments shorter than     299 bp
  25% of sequence in fragments shorter than     350 bp
  30% of sequence in fragments shorter than     406 bp
  35% of sequence in fragments shorter than     461 bp
  40% of sequence in fragments shorter than     517 bp
  45% of sequence in fragments shorter than     575 bp
  50% of sequence in fragments shorter than     635 bp
  55% of sequence in fragments shorter than     702 bp
  60% of sequence in fragments shorter than     774 bp
  65% of sequence in fragments shorter than     859 bp
  70% of sequence in fragments shorter than     946 bp
  75% of sequence in fragments shorter than    1053 bp
  80% of sequence in fragments shorter than    1183 bp
  85% of sequence in fragments shorter than    1351 bp
  90% of sequence in fragments shorter than    1590 bp
  95% of sequence in fragments shorter than    2007 bp

# BsmAI.EsaBC3I (GTCTC+TCGA) double digest, 8234 thousand fragments (6631585+1603346 sites), 361 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     188 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     376 bp
  30% of sequence in fragments shorter than     438 bp
  35% of sequence in fragments shorter than     502 bp
  40% of sequence in fragments shorter than     567 bp
  45% of sequence in fragments shorter than     635 bp
  50% of sequence in fragments shorter than     710 bp
  55% of sequence in fragments shorter than     791 bp
  60% of sequence in fragments shorter than     881 bp
  65% of sequence in fragments shorter than     982 bp
  70% of sequence in fragments shorter than    1097 bp
  75% of sequence in fragments shorter than    1233 bp
  80% of sequence in fragments shorter than    1396 bp
  85% of sequence in fragments shorter than    1613 bp
  90% of sequence in fragments shorter than    1922 bp
  95% of sequence in fragments shorter than    2439 bp

# CviQI.StyI (GTAC+CCWWGG) double digest, 8228 thousand fragments (5115078+3114107 sites), 362 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     300 bp
  25% of sequence in fragments shorter than     349 bp
  30% of sequence in fragments shorter than     402 bp
  35% of sequence in fragments shorter than     456 bp
  40% of sequence in fragments shorter than     511 bp
  45% of sequence in fragments shorter than     568 bp
  50% of sequence in fragments shorter than     629 bp
  55% of sequence in fragments shorter than     692 bp
  60% of sequence in fragments shorter than     757 bp
  65% of sequence in fragments shorter than     835 bp
  70% of sequence in fragments shorter than     919 bp
  75% of sequence in fragments shorter than    1021 bp
  80% of sequence in fragments shorter than    1138 bp
  85% of sequence in fragments shorter than    1294 bp
  90% of sequence in fragments shorter than    1511 bp
  95% of sequence in fragments shorter than    1899 bp

# AciI.Tsp45I (CCGC+GTSAC) double digest, 8227 thousand fragments (4206130+4021761 sites), 362 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     395 bp
  30% of sequence in fragments shorter than     464 bp
  35% of sequence in fragments shorter than     537 bp
  40% of sequence in fragments shorter than     611 bp
  45% of sequence in fragments shorter than     691 bp
  50% of sequence in fragments shorter than     777 bp
  55% of sequence in fragments shorter than     870 bp
  60% of sequence in fragments shorter than     974 bp
  65% of sequence in fragments shorter than    1088 bp
  70% of sequence in fragments shorter than    1223 bp
  75% of sequence in fragments shorter than    1379 bp
  80% of sequence in fragments shorter than    1571 bp
  85% of sequence in fragments shorter than    1821 bp
  90% of sequence in fragments shorter than    2175 bp
  95% of sequence in fragments shorter than    2806 bp

# CviQI.NspI (GTAC+RCATGY) double digest, 8218 thousand fragments (5115078+3104359 sites), 362 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     476 bp
  40% of sequence in fragments shorter than     533 bp
  45% of sequence in fragments shorter than     592 bp
  50% of sequence in fragments shorter than     655 bp
  55% of sequence in fragments shorter than     721 bp
  60% of sequence in fragments shorter than     794 bp
  65% of sequence in fragments shorter than     877 bp
  70% of sequence in fragments shorter than     963 bp
  75% of sequence in fragments shorter than    1068 bp
  80% of sequence in fragments shorter than    1198 bp
  85% of sequence in fragments shorter than    1356 bp
  90% of sequence in fragments shorter than    1587 bp
  95% of sequence in fragments shorter than    2000 bp

# AvaIII.BspNCI (ATGCAT+CCAGA) double digest, 8215 thousand fragments (933357+7282436 sites), 362 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     188 bp
  15% of sequence in fragments shorter than     247 bp
  20% of sequence in fragments shorter than     302 bp
  25% of sequence in fragments shorter than     354 bp
  30% of sequence in fragments shorter than     410 bp
  35% of sequence in fragments shorter than     464 bp
  40% of sequence in fragments shorter than     521 bp
  45% of sequence in fragments shorter than     580 bp
  50% of sequence in fragments shorter than     640 bp
  55% of sequence in fragments shorter than     707 bp
  60% of sequence in fragments shorter than     781 bp
  65% of sequence in fragments shorter than     864 bp
  70% of sequence in fragments shorter than     957 bp
  75% of sequence in fragments shorter than    1065 bp
  80% of sequence in fragments shorter than    1186 bp
  85% of sequence in fragments shorter than    1353 bp
  90% of sequence in fragments shorter than    1591 bp
  95% of sequence in fragments shorter than    2004 bp

# BspNCI.StuI (CCAGA+AGGCCT) double digest, 8207 thousand fragments (7282436+925729 sites), 363 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     187 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     305 bp
  25% of sequence in fragments shorter than     354 bp
  30% of sequence in fragments shorter than     411 bp
  35% of sequence in fragments shorter than     468 bp
  40% of sequence in fragments shorter than     525 bp
  45% of sequence in fragments shorter than     587 bp
  50% of sequence in fragments shorter than     655 bp
  55% of sequence in fragments shorter than     722 bp
  60% of sequence in fragments shorter than     801 bp
  65% of sequence in fragments shorter than     889 bp
  70% of sequence in fragments shorter than     991 bp
  75% of sequence in fragments shorter than    1106 bp
  80% of sequence in fragments shorter than    1239 bp
  85% of sequence in fragments shorter than    1419 bp
  90% of sequence in fragments shorter than    1672 bp
  95% of sequence in fragments shorter than    2097 bp

# AvaI.BspKT6I (CYCGRG+GATC) double digest, 8204 thousand fragments (1005714+7199434 sites), 363 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     238 bp
  20% of sequence in fragments shorter than     296 bp
  25% of sequence in fragments shorter than     349 bp
  30% of sequence in fragments shorter than     406 bp
  35% of sequence in fragments shorter than     460 bp
  40% of sequence in fragments shorter than     516 bp
  45% of sequence in fragments shorter than     578 bp
  50% of sequence in fragments shorter than     642 bp
  55% of sequence in fragments shorter than     711 bp
  60% of sequence in fragments shorter than     789 bp
  65% of sequence in fragments shorter than     873 bp
  70% of sequence in fragments shorter than     970 bp
  75% of sequence in fragments shorter than    1076 bp
  80% of sequence in fragments shorter than    1209 bp
  85% of sequence in fragments shorter than    1383 bp
  90% of sequence in fragments shorter than    1626 bp
  95% of sequence in fragments shorter than    2061 bp

# BspNCI.NdeI (CCAGA+CATATG) double digest, 8185 thousand fragments (7282436+903360 sites), 364 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     189 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     301 bp
  25% of sequence in fragments shorter than     351 bp
  30% of sequence in fragments shorter than     407 bp
  35% of sequence in fragments shorter than     462 bp
  40% of sequence in fragments shorter than     519 bp
  45% of sequence in fragments shorter than     575 bp
  50% of sequence in fragments shorter than     635 bp
  55% of sequence in fragments shorter than     702 bp
  60% of sequence in fragments shorter than     776 bp
  65% of sequence in fragments shorter than     857 bp
  70% of sequence in fragments shorter than     948 bp
  75% of sequence in fragments shorter than    1055 bp
  80% of sequence in fragments shorter than    1184 bp
  85% of sequence in fragments shorter than    1351 bp
  90% of sequence in fragments shorter than    1587 bp
  95% of sequence in fragments shorter than    2001 bp

# BsmAI.HgiCI (GTCTC+GGYRCC) double digest, 8181 thousand fragments (6631585+1550877 sites), 364 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     240 bp
  20% of sequence in fragments shorter than     306 bp
  25% of sequence in fragments shorter than     370 bp
  30% of sequence in fragments shorter than     433 bp
  35% of sequence in fragments shorter than     497 bp
  40% of sequence in fragments shorter than     565 bp
  45% of sequence in fragments shorter than     635 bp
  50% of sequence in fragments shorter than     714 bp
  55% of sequence in fragments shorter than     801 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than    1008 bp
  70% of sequence in fragments shorter than    1132 bp
  75% of sequence in fragments shorter than    1277 bp
  80% of sequence in fragments shorter than    1460 bp
  85% of sequence in fragments shorter than    1691 bp
  90% of sequence in fragments shorter than    2027 bp
  95% of sequence in fragments shorter than    2643 bp

# BsmAI.BsmI (GTCTC+GAATGC) double digest, 8180 thousand fragments (6631585+1549350 sites), 364 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     176 bp
  15% of sequence in fragments shorter than     233 bp
  20% of sequence in fragments shorter than     299 bp
  25% of sequence in fragments shorter than     359 bp
  30% of sequence in fragments shorter than     418 bp
  35% of sequence in fragments shorter than     481 bp
  40% of sequence in fragments shorter than     544 bp
  45% of sequence in fragments shorter than     613 bp
  50% of sequence in fragments shorter than     686 bp
  55% of sequence in fragments shorter than     764 bp
  60% of sequence in fragments shorter than     850 bp
  65% of sequence in fragments shorter than     943 bp
  70% of sequence in fragments shorter than    1051 bp
  75% of sequence in fragments shorter than    1178 bp
  80% of sequence in fragments shorter than    1333 bp
  85% of sequence in fragments shorter than    1534 bp
  90% of sequence in fragments shorter than    1818 bp
  95% of sequence in fragments shorter than    2275 bp

# BspHI.BspKT6I (TCATGA+GATC) double digest, 8177 thousand fragments (978290+7199434 sites), 364 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     180 bp
  15% of sequence in fragments shorter than     241 bp
  20% of sequence in fragments shorter than     297 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     400 bp
  35% of sequence in fragments shorter than     453 bp
  40% of sequence in fragments shorter than     504 bp
  45% of sequence in fragments shorter than     563 bp
  50% of sequence in fragments shorter than     620 bp
  55% of sequence in fragments shorter than     686 bp
  60% of sequence in fragments shorter than     759 bp
  65% of sequence in fragments shorter than     839 bp
  70% of sequence in fragments shorter than     929 bp
  75% of sequence in fragments shorter than    1034 bp
  80% of sequence in fragments shorter than    1159 bp
  85% of sequence in fragments shorter than    1321 bp
  90% of sequence in fragments shorter than    1549 bp
  95% of sequence in fragments shorter than    1955 bp

# AccI.Hin4II (GTMKAC+CCTTC) double digest, 8160 thousand fragments (1101063+7059915 sites), 365 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     257 bp
  20% of sequence in fragments shorter than     316 bp
  25% of sequence in fragments shorter than     370 bp
  30% of sequence in fragments shorter than     427 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     546 bp
  45% of sequence in fragments shorter than     611 bp
  50% of sequence in fragments shorter than     680 bp
  55% of sequence in fragments shorter than     754 bp
  60% of sequence in fragments shorter than     834 bp
  65% of sequence in fragments shorter than     922 bp
  70% of sequence in fragments shorter than    1022 bp
  75% of sequence in fragments shorter than    1136 bp
  80% of sequence in fragments shorter than    1278 bp
  85% of sequence in fragments shorter than    1459 bp
  90% of sequence in fragments shorter than    1710 bp
  95% of sequence in fragments shorter than    2135 bp

# Hin4II.PpuMI (CCTTC+RGGWCCY) double digest, 8144 thousand fragments (7059915+1085138 sites), 365 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     323 bp
  25% of sequence in fragments shorter than     380 bp
  30% of sequence in fragments shorter than     442 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     572 bp
  45% of sequence in fragments shorter than     643 bp
  50% of sequence in fragments shorter than     721 bp
  55% of sequence in fragments shorter than     805 bp
  60% of sequence in fragments shorter than     895 bp
  65% of sequence in fragments shorter than     999 bp
  70% of sequence in fragments shorter than    1110 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1409 bp
  85% of sequence in fragments shorter than    1621 bp
  90% of sequence in fragments shorter than    1911 bp
  95% of sequence in fragments shorter than    2412 bp

# Hin4II.PvuII (CCTTC+CAGCTG) double digest, 8143 thousand fragments (7059915+1084260 sites), 365 bp average fragment length
   5% of sequence in fragments shorter than     124 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     257 bp
  20% of sequence in fragments shorter than     318 bp
  25% of sequence in fragments shorter than     373 bp
  30% of sequence in fragments shorter than     434 bp
  35% of sequence in fragments shorter than     498 bp
  40% of sequence in fragments shorter than     559 bp
  45% of sequence in fragments shorter than     631 bp
  50% of sequence in fragments shorter than     703 bp
  55% of sequence in fragments shorter than     785 bp
  60% of sequence in fragments shorter than     872 bp
  65% of sequence in fragments shorter than     972 bp
  70% of sequence in fragments shorter than    1079 bp
  75% of sequence in fragments shorter than    1212 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1572 bp
  90% of sequence in fragments shorter than    1853 bp
  95% of sequence in fragments shorter than    2333 bp

# BspNCI.HindIII (CCAGA+AAGCTT) double digest, 8142 thousand fragments (7282436+860365 sites), 365 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     189 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     305 bp
  25% of sequence in fragments shorter than     357 bp
  30% of sequence in fragments shorter than     413 bp
  35% of sequence in fragments shorter than     470 bp
  40% of sequence in fragments shorter than     528 bp
  45% of sequence in fragments shorter than     588 bp
  50% of sequence in fragments shorter than     653 bp
  55% of sequence in fragments shorter than     723 bp
  60% of sequence in fragments shorter than     799 bp
  65% of sequence in fragments shorter than     884 bp
  70% of sequence in fragments shorter than     982 bp
  75% of sequence in fragments shorter than    1092 bp
  80% of sequence in fragments shorter than    1219 bp
  85% of sequence in fragments shorter than    1391 bp
  90% of sequence in fragments shorter than    1633 bp
  95% of sequence in fragments shorter than    2053 bp

# ApoI.HhaI (RAATTY+GCGC) double digest, 8138 thousand fragments (6382433+1756502 sites), 366 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     190 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     304 bp
  25% of sequence in fragments shorter than     357 bp
  30% of sequence in fragments shorter than     413 bp
  35% of sequence in fragments shorter than     473 bp
  40% of sequence in fragments shorter than     534 bp
  45% of sequence in fragments shorter than     597 bp
  50% of sequence in fragments shorter than     665 bp
  55% of sequence in fragments shorter than     735 bp
  60% of sequence in fragments shorter than     815 bp
  65% of sequence in fragments shorter than     900 bp
  70% of sequence in fragments shorter than     999 bp
  75% of sequence in fragments shorter than    1114 bp
  80% of sequence in fragments shorter than    1254 bp
  85% of sequence in fragments shorter than    1419 bp
  90% of sequence in fragments shorter than    1662 bp
  95% of sequence in fragments shorter than    2085 bp

# AciI.MlyI (CCGC+GAGTC) double digest, 8137 thousand fragments (4206130+3931692 sites), 366 bp average fragment length
   5% of sequence in fragments shorter than     117 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     332 bp
  25% of sequence in fragments shorter than     404 bp
  30% of sequence in fragments shorter than     479 bp
  35% of sequence in fragments shorter than     557 bp
  40% of sequence in fragments shorter than     638 bp
  45% of sequence in fragments shorter than     724 bp
  50% of sequence in fragments shorter than     817 bp
  55% of sequence in fragments shorter than     913 bp
  60% of sequence in fragments shorter than    1025 bp
  65% of sequence in fragments shorter than    1147 bp
  70% of sequence in fragments shorter than    1287 bp
  75% of sequence in fragments shorter than    1449 bp
  80% of sequence in fragments shorter than    1653 bp
  85% of sequence in fragments shorter than    1913 bp
  90% of sequence in fragments shorter than    2282 bp
  95% of sequence in fragments shorter than    2927 bp

# BspNCI.XbaI (CCAGA+TCTAGA) double digest, 8132 thousand fragments (7282436+850998 sites), 366 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     189 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     305 bp
  25% of sequence in fragments shorter than     356 bp
  30% of sequence in fragments shorter than     412 bp
  35% of sequence in fragments shorter than     466 bp
  40% of sequence in fragments shorter than     525 bp
  45% of sequence in fragments shorter than     586 bp
  50% of sequence in fragments shorter than     648 bp
  55% of sequence in fragments shorter than     717 bp
  60% of sequence in fragments shorter than     794 bp
  65% of sequence in fragments shorter than     880 bp
  70% of sequence in fragments shorter than     978 bp
  75% of sequence in fragments shorter than    1085 bp
  80% of sequence in fragments shorter than    1217 bp
  85% of sequence in fragments shorter than    1389 bp
  90% of sequence in fragments shorter than    1630 bp
  95% of sequence in fragments shorter than    2049 bp

# AvaIII.BspKT6I (ATGCAT+GATC) double digest, 8132 thousand fragments (933357+7199434 sites), 366 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     179 bp
  15% of sequence in fragments shorter than     237 bp
  20% of sequence in fragments shorter than     292 bp
  25% of sequence in fragments shorter than     342 bp
  30% of sequence in fragments shorter than     393 bp
  35% of sequence in fragments shorter than     445 bp
  40% of sequence in fragments shorter than     496 bp
  45% of sequence in fragments shorter than     553 bp
  50% of sequence in fragments shorter than     612 bp
  55% of sequence in fragments shorter than     676 bp
  60% of sequence in fragments shorter than     746 bp
  65% of sequence in fragments shorter than     825 bp
  70% of sequence in fragments shorter than     912 bp
  75% of sequence in fragments shorter than    1016 bp
  80% of sequence in fragments shorter than    1131 bp
  85% of sequence in fragments shorter than    1290 bp
  90% of sequence in fragments shorter than    1510 bp
  95% of sequence in fragments shorter than    1904 bp

# BspNCI.EcoRI (CCAGA+GAATTC) double digest, 8129 thousand fragments (7282436+847401 sites), 366 bp average fragment length
   5% of sequence in fragments shorter than     124 bp
  10% of sequence in fragments shorter than     191 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     309 bp
  25% of sequence in fragments shorter than     359 bp
  30% of sequence in fragments shorter than     416 bp
  35% of sequence in fragments shorter than     474 bp
  40% of sequence in fragments shorter than     531 bp
  45% of sequence in fragments shorter than     589 bp
  50% of sequence in fragments shorter than     654 bp
  55% of sequence in fragments shorter than     723 bp
  60% of sequence in fragments shorter than     798 bp
  65% of sequence in fragments shorter than     883 bp
  70% of sequence in fragments shorter than     982 bp
  75% of sequence in fragments shorter than    1092 bp
  80% of sequence in fragments shorter than    1219 bp
  85% of sequence in fragments shorter than    1391 bp
  90% of sequence in fragments shorter than    1630 bp
  95% of sequence in fragments shorter than    2033 bp

# BspKT6I.StuI (GATC+AGGCCT) double digest, 8124 thousand fragments (7199434+925729 sites), 366 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     178 bp
  15% of sequence in fragments shorter than     237 bp
  20% of sequence in fragments shorter than     293 bp
  25% of sequence in fragments shorter than     344 bp
  30% of sequence in fragments shorter than     393 bp
  35% of sequence in fragments shorter than     446 bp
  40% of sequence in fragments shorter than     498 bp
  45% of sequence in fragments shorter than     556 bp
  50% of sequence in fragments shorter than     617 bp
  55% of sequence in fragments shorter than     681 bp
  60% of sequence in fragments shorter than     749 bp
  65% of sequence in fragments shorter than     828 bp
  70% of sequence in fragments shorter than     915 bp
  75% of sequence in fragments shorter than    1016 bp
  80% of sequence in fragments shorter than    1135 bp
  85% of sequence in fragments shorter than    1290 bp
  90% of sequence in fragments shorter than    1508 bp
  95% of sequence in fragments shorter than    1875 bp

# BsrI.SspI (ACTGG+AATATT) double digest, 8118 thousand fragments (5741310+2377267 sites), 367 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     190 bp
  15% of sequence in fragments shorter than     246 bp
  20% of sequence in fragments shorter than     301 bp
  25% of sequence in fragments shorter than     350 bp
  30% of sequence in fragments shorter than     403 bp
  35% of sequence in fragments shorter than     456 bp
  40% of sequence in fragments shorter than     508 bp
  45% of sequence in fragments shorter than     565 bp
  50% of sequence in fragments shorter than     626 bp
  55% of sequence in fragments shorter than     690 bp
  60% of sequence in fragments shorter than     755 bp
  65% of sequence in fragments shorter than     833 bp
  70% of sequence in fragments shorter than     920 bp
  75% of sequence in fragments shorter than    1024 bp
  80% of sequence in fragments shorter than    1143 bp
  85% of sequence in fragments shorter than    1302 bp
  90% of sequence in fragments shorter than    1520 bp
  95% of sequence in fragments shorter than    1914 bp

# AflIII.BsmAI (ACRYGT+GTCTC) double digest, 8116 thousand fragments (1485394+6631585 sites), 367 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     243 bp
  20% of sequence in fragments shorter than     306 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     424 bp
  35% of sequence in fragments shorter than     485 bp
  40% of sequence in fragments shorter than     546 bp
  45% of sequence in fragments shorter than     610 bp
  50% of sequence in fragments shorter than     680 bp
  55% of sequence in fragments shorter than     755 bp
  60% of sequence in fragments shorter than     838 bp
  65% of sequence in fragments shorter than     931 bp
  70% of sequence in fragments shorter than    1038 bp
  75% of sequence in fragments shorter than    1160 bp
  80% of sequence in fragments shorter than    1311 bp
  85% of sequence in fragments shorter than    1493 bp
  90% of sequence in fragments shorter than    1757 bp
  95% of sequence in fragments shorter than    2227 bp

# BspKT6I.NdeI (GATC+CATATG) double digest, 8102 thousand fragments (7199434+903360 sites), 367 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     179 bp
  15% of sequence in fragments shorter than     238 bp
  20% of sequence in fragments shorter than     294 bp
  25% of sequence in fragments shorter than     344 bp
  30% of sequence in fragments shorter than     397 bp
  35% of sequence in fragments shorter than     449 bp
  40% of sequence in fragments shorter than     499 bp
  45% of sequence in fragments shorter than     556 bp
  50% of sequence in fragments shorter than     617 bp
  55% of sequence in fragments shorter than     682 bp
  60% of sequence in fragments shorter than     754 bp
  65% of sequence in fragments shorter than     833 bp
  70% of sequence in fragments shorter than     918 bp
  75% of sequence in fragments shorter than    1023 bp
  80% of sequence in fragments shorter than    1146 bp
  85% of sequence in fragments shorter than    1307 bp
  90% of sequence in fragments shorter than    1532 bp
  95% of sequence in fragments shorter than    1938 bp

# StyI.TfiI (CCWWGG+GAWTC) double digest, 8097 thousand fragments (3114107+4984362 sites), 367 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     190 bp
  15% of sequence in fragments shorter than     247 bp
  20% of sequence in fragments shorter than     302 bp
  25% of sequence in fragments shorter than     346 bp
  30% of sequence in fragments shorter than     397 bp
  35% of sequence in fragments shorter than     453 bp
  40% of sequence in fragments shorter than     508 bp
  45% of sequence in fragments shorter than     566 bp
  50% of sequence in fragments shorter than     629 bp
  55% of sequence in fragments shorter than     693 bp
  60% of sequence in fragments shorter than     766 bp
  65% of sequence in fragments shorter than     842 bp
  70% of sequence in fragments shorter than     931 bp
  75% of sequence in fragments shorter than    1034 bp
  80% of sequence in fragments shorter than    1159 bp
  85% of sequence in fragments shorter than    1316 bp
  90% of sequence in fragments shorter than    1534 bp
  95% of sequence in fragments shorter than    1903 bp

# ApoI.BfiI (RAATTY+ACTGGG) double digest, 8088 thousand fragments (6382433+1706594 sites), 368 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     191 bp
  15% of sequence in fragments shorter than     249 bp
  20% of sequence in fragments shorter than     302 bp
  25% of sequence in fragments shorter than     353 bp
  30% of sequence in fragments shorter than     405 bp
  35% of sequence in fragments shorter than     463 bp
  40% of sequence in fragments shorter than     521 bp
  45% of sequence in fragments shorter than     582 bp
  50% of sequence in fragments shorter than     646 bp
  55% of sequence in fragments shorter than     714 bp
  60% of sequence in fragments shorter than     790 bp
  65% of sequence in fragments shorter than     876 bp
  70% of sequence in fragments shorter than     972 bp
  75% of sequence in fragments shorter than    1086 bp
  80% of sequence in fragments shorter than    1226 bp
  85% of sequence in fragments shorter than    1390 bp
  90% of sequence in fragments shorter than    1647 bp
  95% of sequence in fragments shorter than    2095 bp

# NspI.TfiI (RCATGY+GAWTC) double digest, 8088 thousand fragments (3104359+4984362 sites), 368 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     306 bp
  25% of sequence in fragments shorter than     347 bp
  30% of sequence in fragments shorter than     397 bp
  35% of sequence in fragments shorter than     450 bp
  40% of sequence in fragments shorter than     505 bp
  45% of sequence in fragments shorter than     564 bp
  50% of sequence in fragments shorter than     626 bp
  55% of sequence in fragments shorter than     690 bp
  60% of sequence in fragments shorter than     760 bp
  65% of sequence in fragments shorter than     836 bp
  70% of sequence in fragments shorter than     924 bp
  75% of sequence in fragments shorter than    1027 bp
  80% of sequence in fragments shorter than    1148 bp
  85% of sequence in fragments shorter than    1304 bp
  90% of sequence in fragments shorter than    1522 bp
  95% of sequence in fragments shorter than    1887 bp

# BsmAI.FauI (GTCTC+CCCGC) double digest, 8070 thousand fragments (6631585+1439772 sites), 369 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     320 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     458 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     602 bp
  45% of sequence in fragments shorter than     681 bp
  50% of sequence in fragments shorter than     768 bp
  55% of sequence in fragments shorter than     864 bp
  60% of sequence in fragments shorter than     969 bp
  65% of sequence in fragments shorter than    1087 bp
  70% of sequence in fragments shorter than    1223 bp
  75% of sequence in fragments shorter than    1382 bp
  80% of sequence in fragments shorter than    1575 bp
  85% of sequence in fragments shorter than    1823 bp
  90% of sequence in fragments shorter than    2169 bp
  95% of sequence in fragments shorter than    2797 bp

# BsmAI.BspMI (GTCTC+ACCTGC) double digest, 8069 thousand fragments (6631585+1438129 sites), 369 bp average fragment length
   5% of sequence in fragments shorter than     129 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     243 bp
  20% of sequence in fragments shorter than     307 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     429 bp
  35% of sequence in fragments shorter than     494 bp
  40% of sequence in fragments shorter than     559 bp
  45% of sequence in fragments shorter than     627 bp
  50% of sequence in fragments shorter than     701 bp
  55% of sequence in fragments shorter than     784 bp
  60% of sequence in fragments shorter than     872 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1090 bp
  75% of sequence in fragments shorter than    1226 bp
  80% of sequence in fragments shorter than    1386 bp
  85% of sequence in fragments shorter than    1603 bp
  90% of sequence in fragments shorter than    1878 bp
  95% of sequence in fragments shorter than    2383 bp

# AvaI.Hin4II (CYCGRG+CCTTC) double digest, 8065 thousand fragments (1005714+7059915 sites), 369 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     321 bp
  25% of sequence in fragments shorter than     374 bp
  30% of sequence in fragments shorter than     434 bp
  35% of sequence in fragments shorter than     496 bp
  40% of sequence in fragments shorter than     555 bp
  45% of sequence in fragments shorter than     625 bp
  50% of sequence in fragments shorter than     696 bp
  55% of sequence in fragments shorter than     775 bp
  60% of sequence in fragments shorter than     861 bp
  65% of sequence in fragments shorter than     957 bp
  70% of sequence in fragments shorter than    1061 bp
  75% of sequence in fragments shorter than    1190 bp
  80% of sequence in fragments shorter than    1346 bp
  85% of sequence in fragments shorter than    1542 bp
  90% of sequence in fragments shorter than    1816 bp
  95% of sequence in fragments shorter than    2286 bp

# BspKT6I.HindIII (GATC+AAGCTT) double digest, 8059 thousand fragments (7199434+860365 sites), 369 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     179 bp
  15% of sequence in fragments shorter than     239 bp
  20% of sequence in fragments shorter than     295 bp
  25% of sequence in fragments shorter than     346 bp
  30% of sequence in fragments shorter than     398 bp
  35% of sequence in fragments shorter than     451 bp
  40% of sequence in fragments shorter than     502 bp
  45% of sequence in fragments shorter than     561 bp
  50% of sequence in fragments shorter than     621 bp
  55% of sequence in fragments shorter than     686 bp
  60% of sequence in fragments shorter than     757 bp
  65% of sequence in fragments shorter than     837 bp
  70% of sequence in fragments shorter than     927 bp
  75% of sequence in fragments shorter than    1029 bp
  80% of sequence in fragments shorter than    1146 bp
  85% of sequence in fragments shorter than    1303 bp
  90% of sequence in fragments shorter than    1523 bp
  95% of sequence in fragments shorter than    1908 bp

# BsrI.HpaII (ACTGG+CCGG) double digest, 8058 thousand fragments (5741310+2317722 sites), 369 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     187 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     428 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     552 bp
  45% of sequence in fragments shorter than     620 bp
  50% of sequence in fragments shorter than     689 bp
  55% of sequence in fragments shorter than     759 bp
  60% of sequence in fragments shorter than     844 bp
  65% of sequence in fragments shorter than     937 bp
  70% of sequence in fragments shorter than    1044 bp
  75% of sequence in fragments shorter than    1171 bp
  80% of sequence in fragments shorter than    1327 bp
  85% of sequence in fragments shorter than    1520 bp
  90% of sequence in fragments shorter than    1803 bp
  95% of sequence in fragments shorter than    2320 bp

# BglII.BspNCI (AGATCT+CCAGA) double digest, 8056 thousand fragments (774732+7282436 sites), 369 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     424 bp
  35% of sequence in fragments shorter than     481 bp
  40% of sequence in fragments shorter than     539 bp
  45% of sequence in fragments shorter than     599 bp
  50% of sequence in fragments shorter than     666 bp
  55% of sequence in fragments shorter than     735 bp
  60% of sequence in fragments shorter than     815 bp
  65% of sequence in fragments shorter than     901 bp
  70% of sequence in fragments shorter than    1001 bp
  75% of sequence in fragments shorter than    1116 bp
  80% of sequence in fragments shorter than    1247 bp
  85% of sequence in fragments shorter than    1424 bp
  90% of sequence in fragments shorter than    1681 bp
  95% of sequence in fragments shorter than    2123 bp

# BspKT6I.XbaI (GATC+TCTAGA) double digest, 8049 thousand fragments (7199434+850998 sites), 370 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     181 bp
  15% of sequence in fragments shorter than     244 bp
  20% of sequence in fragments shorter than     300 bp
  25% of sequence in fragments shorter than     349 bp
  30% of sequence in fragments shorter than     403 bp
  35% of sequence in fragments shorter than     455 bp
  40% of sequence in fragments shorter than     508 bp
  45% of sequence in fragments shorter than     567 bp
  50% of sequence in fragments shorter than     626 bp
  55% of sequence in fragments shorter than     690 bp
  60% of sequence in fragments shorter than     763 bp
  65% of sequence in fragments shorter than     843 bp
  70% of sequence in fragments shorter than     933 bp
  75% of sequence in fragments shorter than    1036 bp
  80% of sequence in fragments shorter than    1160 bp
  85% of sequence in fragments shorter than    1318 bp
  90% of sequence in fragments shorter than    1539 bp
  95% of sequence in fragments shorter than    1913 bp

# BspKT6I.EcoRI (GATC+GAATTC) double digest, 8046 thousand fragments (7199434+847401 sites), 370 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     180 bp
  15% of sequence in fragments shorter than     241 bp
  20% of sequence in fragments shorter than     295 bp
  25% of sequence in fragments shorter than     345 bp
  30% of sequence in fragments shorter than     396 bp
  35% of sequence in fragments shorter than     450 bp
  40% of sequence in fragments shorter than     502 bp
  45% of sequence in fragments shorter than     561 bp
  50% of sequence in fragments shorter than     622 bp
  55% of sequence in fragments shorter than     686 bp
  60% of sequence in fragments shorter than     756 bp
  65% of sequence in fragments shorter than     836 bp
  70% of sequence in fragments shorter than     924 bp
  75% of sequence in fragments shorter than    1025 bp
  80% of sequence in fragments shorter than    1147 bp
  85% of sequence in fragments shorter than    1302 bp
  90% of sequence in fragments shorter than    1515 bp
  95% of sequence in fragments shorter than    1894 bp

# BspNCI.NcoI (CCAGA+CCATGG) double digest, 8041 thousand fragments (7282436+759594 sites), 370 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     190 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     309 bp
  25% of sequence in fragments shorter than     363 bp
  30% of sequence in fragments shorter than     421 bp
  35% of sequence in fragments shorter than     480 bp
  40% of sequence in fragments shorter than     538 bp
  45% of sequence in fragments shorter than     596 bp
  50% of sequence in fragments shorter than     663 bp
  55% of sequence in fragments shorter than     735 bp
  60% of sequence in fragments shorter than     816 bp
  65% of sequence in fragments shorter than     905 bp
  70% of sequence in fragments shorter than    1008 bp
  75% of sequence in fragments shorter than    1125 bp
  80% of sequence in fragments shorter than    1259 bp
  85% of sequence in fragments shorter than    1443 bp
  90% of sequence in fragments shorter than    1703 bp
  95% of sequence in fragments shorter than    2157 bp

# BspHI.Hin4II (TCATGA+CCTTC) double digest, 8037 thousand fragments (978290+7059915 sites), 370 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     263 bp
  20% of sequence in fragments shorter than     324 bp
  25% of sequence in fragments shorter than     379 bp
  30% of sequence in fragments shorter than     437 bp
  35% of sequence in fragments shorter than     500 bp
  40% of sequence in fragments shorter than     561 bp
  45% of sequence in fragments shorter than     629 bp
  50% of sequence in fragments shorter than     698 bp
  55% of sequence in fragments shorter than     776 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     952 bp
  70% of sequence in fragments shorter than    1054 bp
  75% of sequence in fragments shorter than    1177 bp
  80% of sequence in fragments shorter than    1325 bp
  85% of sequence in fragments shorter than    1510 bp
  90% of sequence in fragments shorter than    1772 bp
  95% of sequence in fragments shorter than    2212 bp

# AvaII.BbvI (GGWCC+GCAGC) double digest, 8037 thousand fragments (2747690+5290051 sites), 370 bp average fragment length
   5% of sequence in fragments shorter than     118 bp
  10% of sequence in fragments shorter than     190 bp
  15% of sequence in fragments shorter than     259 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     396 bp
  30% of sequence in fragments shorter than     470 bp
  35% of sequence in fragments shorter than     548 bp
  40% of sequence in fragments shorter than     629 bp
  45% of sequence in fragments shorter than     710 bp
  50% of sequence in fragments shorter than     803 bp
  55% of sequence in fragments shorter than     899 bp
  60% of sequence in fragments shorter than    1000 bp
  65% of sequence in fragments shorter than    1123 bp
  70% of sequence in fragments shorter than    1262 bp
  75% of sequence in fragments shorter than    1425 bp
  80% of sequence in fragments shorter than    1625 bp
  85% of sequence in fragments shorter than    1879 bp
  90% of sequence in fragments shorter than    2244 bp
  95% of sequence in fragments shorter than    2881 bp

# ApoI.PsiI (RAATTY+TTATAA) double digest, 8029 thousand fragments (6382433+1647912 sites), 371 bp average fragment length
   5% of sequence in fragments shorter than     120 bp
  10% of sequence in fragments shorter than     188 bp
  15% of sequence in fragments shorter than     249 bp
  20% of sequence in fragments shorter than     307 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     425 bp
  35% of sequence in fragments shorter than     490 bp
  40% of sequence in fragments shorter than     558 bp
  45% of sequence in fragments shorter than     630 bp
  50% of sequence in fragments shorter than     704 bp
  55% of sequence in fragments shorter than     792 bp
  60% of sequence in fragments shorter than     888 bp
  65% of sequence in fragments shorter than     999 bp
  70% of sequence in fragments shorter than    1129 bp
  75% of sequence in fragments shorter than    1287 bp
  80% of sequence in fragments shorter than    1471 bp
  85% of sequence in fragments shorter than    1747 bp
  90% of sequence in fragments shorter than    2176 bp
  95% of sequence in fragments shorter than    3097 bp

# BclI.BspNCI (TGATCA+CCAGA) double digest, 8020 thousand fragments (738785+7282436 sites), 371 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     360 bp
  30% of sequence in fragments shorter than     417 bp
  35% of sequence in fragments shorter than     475 bp
  40% of sequence in fragments shorter than     533 bp
  45% of sequence in fragments shorter than     593 bp
  50% of sequence in fragments shorter than     658 bp
  55% of sequence in fragments shorter than     727 bp
  60% of sequence in fragments shorter than     804 bp
  65% of sequence in fragments shorter than     891 bp
  70% of sequence in fragments shorter than     990 bp
  75% of sequence in fragments shorter than    1102 bp
  80% of sequence in fragments shorter than    1229 bp
  85% of sequence in fragments shorter than    1402 bp
  90% of sequence in fragments shorter than    1652 bp
  95% of sequence in fragments shorter than    2085 bp

# BbvI.SmlI (GCAGC+CTYRAG) double digest, 8017 thousand fragments (5290051+2727867 sites), 371 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     259 bp
  20% of sequence in fragments shorter than     320 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     446 bp
  35% of sequence in fragments shorter than     510 bp
  40% of sequence in fragments shorter than     578 bp
  45% of sequence in fragments shorter than     650 bp
  50% of sequence in fragments shorter than     726 bp
  55% of sequence in fragments shorter than     810 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than     999 bp
  70% of sequence in fragments shorter than    1115 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1415 bp
  85% of sequence in fragments shorter than    1632 bp
  90% of sequence in fragments shorter than    1932 bp
  95% of sequence in fragments shorter than    2438 bp

# BspNCI.FnuDII (CCAGA+CGCG) double digest, 8015 thousand fragments (7282436+733940 sites), 371 bp average fragment length
   5% of sequence in fragments shorter than     124 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     255 bp
  20% of sequence in fragments shorter than     315 bp
  25% of sequence in fragments shorter than     371 bp
  30% of sequence in fragments shorter than     430 bp
  35% of sequence in fragments shorter than     491 bp
  40% of sequence in fragments shorter than     552 bp
  45% of sequence in fragments shorter than     613 bp
  50% of sequence in fragments shorter than     682 bp
  55% of sequence in fragments shorter than     755 bp
  60% of sequence in fragments shorter than     840 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1036 bp
  75% of sequence in fragments shorter than    1147 bp
  80% of sequence in fragments shorter than    1293 bp
  85% of sequence in fragments shorter than    1482 bp
  90% of sequence in fragments shorter than    1747 bp
  95% of sequence in fragments shorter than    2215 bp

# BsaAI.BspNCI (YACGTR+CCAGA) double digest, 7995 thousand fragments (713801+7282436 sites), 372 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     253 bp
  20% of sequence in fragments shorter than     310 bp
  25% of sequence in fragments shorter than     364 bp
  30% of sequence in fragments shorter than     421 bp
  35% of sequence in fragments shorter than     477 bp
  40% of sequence in fragments shorter than     536 bp
  45% of sequence in fragments shorter than     595 bp
  50% of sequence in fragments shorter than     660 bp
  55% of sequence in fragments shorter than     730 bp
  60% of sequence in fragments shorter than     810 bp
  65% of sequence in fragments shorter than     895 bp
  70% of sequence in fragments shorter than     996 bp
  75% of sequence in fragments shorter than    1108 bp
  80% of sequence in fragments shorter than    1237 bp
  85% of sequence in fragments shorter than    1414 bp
  90% of sequence in fragments shorter than    1665 bp
  95% of sequence in fragments shorter than    2089 bp

# AvaIII.Hin4II (ATGCAT+CCTTC) double digest, 7992 thousand fragments (933357+7059915 sites), 372 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     324 bp
  25% of sequence in fragments shorter than     378 bp
  30% of sequence in fragments shorter than     436 bp
  35% of sequence in fragments shorter than     498 bp
  40% of sequence in fragments shorter than     556 bp
  45% of sequence in fragments shorter than     624 bp
  50% of sequence in fragments shorter than     693 bp
  55% of sequence in fragments shorter than     770 bp
  60% of sequence in fragments shorter than     854 bp
  65% of sequence in fragments shorter than     945 bp
  70% of sequence in fragments shorter than    1045 bp
  75% of sequence in fragments shorter than    1167 bp
  80% of sequence in fragments shorter than    1313 bp
  85% of sequence in fragments shorter than    1499 bp
  90% of sequence in fragments shorter than    1757 bp
  95% of sequence in fragments shorter than    2200 bp

# BceAI.BspNCI (ACGGC+CCAGA) double digest, 7987 thousand fragments (705178+7282436 sites), 373 bp average fragment length
   5% of sequence in fragments shorter than     124 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     253 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     426 bp
  35% of sequence in fragments shorter than     486 bp
  40% of sequence in fragments shorter than     547 bp
  45% of sequence in fragments shorter than     608 bp
  50% of sequence in fragments shorter than     676 bp
  55% of sequence in fragments shorter than     748 bp
  60% of sequence in fragments shorter than     832 bp
  65% of sequence in fragments shorter than     921 bp
  70% of sequence in fragments shorter than    1027 bp
  75% of sequence in fragments shorter than    1143 bp
  80% of sequence in fragments shorter than    1282 bp
  85% of sequence in fragments shorter than    1469 bp
  90% of sequence in fragments shorter than    1732 bp
  95% of sequence in fragments shorter than    2188 bp

# ApoI.EsaBC3I (RAATTY+TCGA) double digest, 7985 thousand fragments (6382433+1603346 sites), 373 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     307 bp
  25% of sequence in fragments shorter than     361 bp
  30% of sequence in fragments shorter than     417 bp
  35% of sequence in fragments shorter than     475 bp
  40% of sequence in fragments shorter than     536 bp
  45% of sequence in fragments shorter than     599 bp
  50% of sequence in fragments shorter than     667 bp
  55% of sequence in fragments shorter than     739 bp
  60% of sequence in fragments shorter than     821 bp
  65% of sequence in fragments shorter than     907 bp
  70% of sequence in fragments shorter than    1009 bp
  75% of sequence in fragments shorter than    1128 bp
  80% of sequence in fragments shorter than    1274 bp
  85% of sequence in fragments shorter than    1445 bp
  90% of sequence in fragments shorter than    1712 bp
  95% of sequence in fragments shorter than    2191 bp

# Hin4II.StuI (CCTTC+AGGCCT) double digest, 7985 thousand fragments (7059915+925729 sites), 373 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     269 bp
  20% of sequence in fragments shorter than     332 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     450 bp
  35% of sequence in fragments shorter than     516 bp
  40% of sequence in fragments shorter than     581 bp
  45% of sequence in fragments shorter than     654 bp
  50% of sequence in fragments shorter than     731 bp
  55% of sequence in fragments shorter than     815 bp
  60% of sequence in fragments shorter than     908 bp
  65% of sequence in fragments shorter than    1011 bp
  70% of sequence in fragments shorter than    1126 bp
  75% of sequence in fragments shorter than    1263 bp
  80% of sequence in fragments shorter than    1426 bp
  85% of sequence in fragments shorter than    1638 bp
  90% of sequence in fragments shorter than    1933 bp
  95% of sequence in fragments shorter than    2432 bp

# BciVI.BspNCI (GTATCC+CCAGA) double digest, 7978 thousand fragments (696093+7282436 sites), 373 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     424 bp
  35% of sequence in fragments shorter than     482 bp
  40% of sequence in fragments shorter than     541 bp
  45% of sequence in fragments shorter than     600 bp
  50% of sequence in fragments shorter than     666 bp
  55% of sequence in fragments shorter than     736 bp
  60% of sequence in fragments shorter than     812 bp
  65% of sequence in fragments shorter than     897 bp
  70% of sequence in fragments shorter than     994 bp
  75% of sequence in fragments shorter than    1106 bp
  80% of sequence in fragments shorter than    1245 bp
  85% of sequence in fragments shorter than    1424 bp
  90% of sequence in fragments shorter than    1679 bp
  95% of sequence in fragments shorter than    2118 bp

# BglII.BspKT6I (AGATCT+GATC) double digest, 7973 thousand fragments (774732+7199434 sites), 373 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     634 bp
  50% of sequence in fragments shorter than     703 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     862 bp
  65% of sequence in fragments shorter than     952 bp
  70% of sequence in fragments shorter than    1050 bp
  75% of sequence in fragments shorter than    1166 bp
  80% of sequence in fragments shorter than    1309 bp
  85% of sequence in fragments shorter than    1491 bp
  90% of sequence in fragments shorter than    1749 bp
  95% of sequence in fragments shorter than    2213 bp

# BthCI.HhaI (GCNGC+GCGC) double digest, 7965 thousand fragments (6209929+1756502 sites), 374 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     289 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     444 bp
  30% of sequence in fragments shorter than     528 bp
  35% of sequence in fragments shorter than     616 bp
  40% of sequence in fragments shorter than     704 bp
  45% of sequence in fragments shorter than     804 bp
  50% of sequence in fragments shorter than     905 bp
  55% of sequence in fragments shorter than    1020 bp
  60% of sequence in fragments shorter than    1146 bp
  65% of sequence in fragments shorter than    1292 bp
  70% of sequence in fragments shorter than    1457 bp
  75% of sequence in fragments shorter than    1643 bp
  80% of sequence in fragments shorter than    1875 bp
  85% of sequence in fragments shorter than    2165 bp
  90% of sequence in fragments shorter than    2596 bp
  95% of sequence in fragments shorter than    3326 bp

# Hin4II.NdeI (CCTTC+CATATG) double digest, 7962 thousand fragments (7059915+903360 sites), 374 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     323 bp
  25% of sequence in fragments shorter than     378 bp
  30% of sequence in fragments shorter than     437 bp
  35% of sequence in fragments shorter than     499 bp
  40% of sequence in fragments shorter than     561 bp
  45% of sequence in fragments shorter than     628 bp
  50% of sequence in fragments shorter than     697 bp
  55% of sequence in fragments shorter than     773 bp
  60% of sequence in fragments shorter than     857 bp
  65% of sequence in fragments shorter than     948 bp
  70% of sequence in fragments shorter than    1048 bp
  75% of sequence in fragments shorter than    1168 bp
  80% of sequence in fragments shorter than    1314 bp
  85% of sequence in fragments shorter than    1498 bp
  90% of sequence in fragments shorter than    1754 bp
  95% of sequence in fragments shorter than    2190 bp

# BspKT6I.NcoI (GATC+CCATGG) double digest, 7958 thousand fragments (7199434+759594 sites), 374 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     181 bp
  15% of sequence in fragments shorter than     242 bp
  20% of sequence in fragments shorter than     299 bp
  25% of sequence in fragments shorter than     350 bp
  30% of sequence in fragments shorter than     404 bp
  35% of sequence in fragments shorter than     458 bp
  40% of sequence in fragments shorter than     510 bp
  45% of sequence in fragments shorter than     569 bp
  50% of sequence in fragments shorter than     631 bp
  55% of sequence in fragments shorter than     697 bp
  60% of sequence in fragments shorter than     771 bp
  65% of sequence in fragments shorter than     853 bp
  70% of sequence in fragments shorter than     945 bp
  75% of sequence in fragments shorter than    1047 bp
  80% of sequence in fragments shorter than    1174 bp
  85% of sequence in fragments shorter than    1341 bp
  90% of sequence in fragments shorter than    1577 bp
  95% of sequence in fragments shorter than    1997 bp

# MlyI.Tsp45I (GAGTC+GTSAC) double digest, 7952 thousand fragments (3931692+4021761 sites), 374 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     323 bp
  25% of sequence in fragments shorter than     375 bp
  30% of sequence in fragments shorter than     431 bp
  35% of sequence in fragments shorter than     489 bp
  40% of sequence in fragments shorter than     549 bp
  45% of sequence in fragments shorter than     612 bp
  50% of sequence in fragments shorter than     680 bp
  55% of sequence in fragments shorter than     752 bp
  60% of sequence in fragments shorter than     834 bp
  65% of sequence in fragments shorter than     921 bp
  70% of sequence in fragments shorter than    1024 bp
  75% of sequence in fragments shorter than    1146 bp
  80% of sequence in fragments shorter than    1297 bp
  85% of sequence in fragments shorter than    1495 bp
  90% of sequence in fragments shorter than    1791 bp
  95% of sequence in fragments shorter than    2356 bp

# BsmAI.PstI (GTCTC+CTGCAG) double digest, 7952 thousand fragments (6631585+1321469 sites), 374 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     314 bp
  25% of sequence in fragments shorter than     378 bp
  30% of sequence in fragments shorter than     443 bp
  35% of sequence in fragments shorter than     509 bp
  40% of sequence in fragments shorter than     576 bp
  45% of sequence in fragments shorter than     646 bp
  50% of sequence in fragments shorter than     722 bp
  55% of sequence in fragments shorter than     809 bp
  60% of sequence in fragments shorter than     904 bp
  65% of sequence in fragments shorter than    1012 bp
  70% of sequence in fragments shorter than    1134 bp
  75% of sequence in fragments shorter than    1280 bp
  80% of sequence in fragments shorter than    1455 bp
  85% of sequence in fragments shorter than    1693 bp
  90% of sequence in fragments shorter than    2031 bp
  95% of sequence in fragments shorter than    2599 bp

# AvaII.FokI (GGWCC+GGATG) double digest, 7948 thousand fragments (2747690+5201116 sites), 374 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     257 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     371 bp
  30% of sequence in fragments shorter than     434 bp
  35% of sequence in fragments shorter than     499 bp
  40% of sequence in fragments shorter than     565 bp
  45% of sequence in fragments shorter than     636 bp
  50% of sequence in fragments shorter than     710 bp
  55% of sequence in fragments shorter than     792 bp
  60% of sequence in fragments shorter than     882 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1084 bp
  75% of sequence in fragments shorter than    1209 bp
  80% of sequence in fragments shorter than    1364 bp
  85% of sequence in fragments shorter than    1564 bp
  90% of sequence in fragments shorter than    1847 bp
  95% of sequence in fragments shorter than    2349 bp

# BclI.BspKT6I (TGATCA+GATC) double digest, 7937 thousand fragments (738785+7199434 sites), 375 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     634 bp
  50% of sequence in fragments shorter than     703 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     861 bp
  65% of sequence in fragments shorter than     952 bp
  70% of sequence in fragments shorter than    1050 bp
  75% of sequence in fragments shorter than    1166 bp
  80% of sequence in fragments shorter than    1309 bp
  85% of sequence in fragments shorter than    1491 bp
  90% of sequence in fragments shorter than    1749 bp
  95% of sequence in fragments shorter than    2213 bp

# BspKT6I.FnuDII (GATC+CGCG) double digest, 7932 thousand fragments (7199434+733940 sites), 375 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     424 bp
  35% of sequence in fragments shorter than     479 bp
  40% of sequence in fragments shorter than     539 bp
  45% of sequence in fragments shorter than     602 bp
  50% of sequence in fragments shorter than     669 bp
  55% of sequence in fragments shorter than     741 bp
  60% of sequence in fragments shorter than     821 bp
  65% of sequence in fragments shorter than     908 bp
  70% of sequence in fragments shorter than    1008 bp
  75% of sequence in fragments shorter than    1115 bp
  80% of sequence in fragments shorter than    1253 bp
  85% of sequence in fragments shorter than    1427 bp
  90% of sequence in fragments shorter than    1681 bp
  95% of sequence in fragments shorter than    2121 bp

# ApoI.HgiCI (RAATTY+GGYRCC) double digest, 7932 thousand fragments (6382433+1550877 sites), 375 bp average fragment length
   5% of sequence in fragments shorter than     129 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     305 bp
  25% of sequence in fragments shorter than     358 bp
  30% of sequence in fragments shorter than     412 bp
  35% of sequence in fragments shorter than     468 bp
  40% of sequence in fragments shorter than     526 bp
  45% of sequence in fragments shorter than     588 bp
  50% of sequence in fragments shorter than     653 bp
  55% of sequence in fragments shorter than     722 bp
  60% of sequence in fragments shorter than     800 bp
  65% of sequence in fragments shorter than     880 bp
  70% of sequence in fragments shorter than     972 bp
  75% of sequence in fragments shorter than    1082 bp
  80% of sequence in fragments shorter than    1216 bp
  85% of sequence in fragments shorter than    1383 bp
  90% of sequence in fragments shorter than    1625 bp
  95% of sequence in fragments shorter than    2054 bp

# ApoI.BsmI (RAATTY+GAATGC) double digest, 7931 thousand fragments (6382433+1549350 sites), 375 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     247 bp
  20% of sequence in fragments shorter than     305 bp
  25% of sequence in fragments shorter than     361 bp
  30% of sequence in fragments shorter than     422 bp
  35% of sequence in fragments shorter than     487 bp
  40% of sequence in fragments shorter than     553 bp
  45% of sequence in fragments shorter than     622 bp
  50% of sequence in fragments shorter than     696 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     867 bp
  65% of sequence in fragments shorter than     958 bp
  70% of sequence in fragments shorter than    1071 bp
  75% of sequence in fragments shorter than    1204 bp
  80% of sequence in fragments shorter than    1371 bp
  85% of sequence in fragments shorter than    1590 bp
  90% of sequence in fragments shorter than    1916 bp
  95% of sequence in fragments shorter than    2536 bp

# FokI.SmlI (GGATG+CTYRAG) double digest, 7928 thousand fragments (5201116+2727867 sites), 375 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     256 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     364 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     476 bp
  40% of sequence in fragments shorter than     533 bp
  45% of sequence in fragments shorter than     594 bp
  50% of sequence in fragments shorter than     657 bp
  55% of sequence in fragments shorter than     725 bp
  60% of sequence in fragments shorter than     802 bp
  65% of sequence in fragments shorter than     885 bp
  70% of sequence in fragments shorter than     977 bp
  75% of sequence in fragments shorter than    1085 bp
  80% of sequence in fragments shorter than    1216 bp
  85% of sequence in fragments shorter than    1386 bp
  90% of sequence in fragments shorter than    1619 bp
  95% of sequence in fragments shorter than    2035 bp

# BccI.HhaI (CCATC+GCGC) double digest, 7926 thousand fragments (6170927+1756502 sites), 375 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     323 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     444 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     577 bp
  45% of sequence in fragments shorter than     647 bp
  50% of sequence in fragments shorter than     719 bp
  55% of sequence in fragments shorter than     803 bp
  60% of sequence in fragments shorter than     894 bp
  65% of sequence in fragments shorter than     998 bp
  70% of sequence in fragments shorter than    1106 bp
  75% of sequence in fragments shorter than    1241 bp
  80% of sequence in fragments shorter than    1404 bp
  85% of sequence in fragments shorter than    1617 bp
  90% of sequence in fragments shorter than    1917 bp
  95% of sequence in fragments shorter than    2435 bp

# AflII.BspNCI (CTTAAG+CCAGA) double digest, 7925 thousand fragments (643284+7282436 sites), 376 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     255 bp
  20% of sequence in fragments shorter than     314 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     425 bp
  35% of sequence in fragments shorter than     484 bp
  40% of sequence in fragments shorter than     543 bp
  45% of sequence in fragments shorter than     602 bp
  50% of sequence in fragments shorter than     668 bp
  55% of sequence in fragments shorter than     737 bp
  60% of sequence in fragments shorter than     817 bp
  65% of sequence in fragments shorter than     903 bp
  70% of sequence in fragments shorter than    1003 bp
  75% of sequence in fragments shorter than    1109 bp
  80% of sequence in fragments shorter than    1246 bp
  85% of sequence in fragments shorter than    1421 bp
  90% of sequence in fragments shorter than    1675 bp
  95% of sequence in fragments shorter than    2117 bp

# Hin4II.HindIII (CCTTC+AAGCTT) double digest, 7919 thousand fragments (7059915+860365 sites), 376 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     202 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     327 bp
  25% of sequence in fragments shorter than     383 bp
  30% of sequence in fragments shorter than     443 bp
  35% of sequence in fragments shorter than     507 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     638 bp
  50% of sequence in fragments shorter than     709 bp
  55% of sequence in fragments shorter than     789 bp
  60% of sequence in fragments shorter than     875 bp
  65% of sequence in fragments shorter than     971 bp
  70% of sequence in fragments shorter than    1076 bp
  75% of sequence in fragments shorter than    1203 bp
  80% of sequence in fragments shorter than    1356 bp
  85% of sequence in fragments shorter than    1548 bp
  90% of sequence in fragments shorter than    1817 bp
  95% of sequence in fragments shorter than    2275 bp

# BfiI.BthCI (ACTGGG+GCNGC) double digest, 7916 thousand fragments (1706594+6209929 sites), 376 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     202 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     423 bp
  30% of sequence in fragments shorter than     502 bp
  35% of sequence in fragments shorter than     582 bp
  40% of sequence in fragments shorter than     667 bp
  45% of sequence in fragments shorter than     758 bp
  50% of sequence in fragments shorter than     853 bp
  55% of sequence in fragments shorter than     950 bp
  60% of sequence in fragments shorter than    1064 bp
  65% of sequence in fragments shorter than    1190 bp
  70% of sequence in fragments shorter than    1335 bp
  75% of sequence in fragments shorter than    1509 bp
  80% of sequence in fragments shorter than    1711 bp
  85% of sequence in fragments shorter than    1975 bp
  90% of sequence in fragments shorter than    2353 bp
  95% of sequence in fragments shorter than    2990 bp

# BsaAI.BspKT6I (YACGTR+GATC) double digest, 7912 thousand fragments (713801+7199434 sites), 376 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     302 bp
  25% of sequence in fragments shorter than     354 bp
  30% of sequence in fragments shorter than     408 bp
  35% of sequence in fragments shorter than     461 bp
  40% of sequence in fragments shorter than     515 bp
  45% of sequence in fragments shorter than     573 bp
  50% of sequence in fragments shorter than     635 bp
  55% of sequence in fragments shorter than     702 bp
  60% of sequence in fragments shorter than     776 bp
  65% of sequence in fragments shorter than     859 bp
  70% of sequence in fragments shorter than     949 bp
  75% of sequence in fragments shorter than    1053 bp
  80% of sequence in fragments shorter than    1177 bp
  85% of sequence in fragments shorter than    1342 bp
  90% of sequence in fragments shorter than    1572 bp
  95% of sequence in fragments shorter than    1980 bp

# BspNCI.HaeII (CCAGA+RGCGCY) double digest, 7912 thousand fragments (7282436+630101 sites), 376 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     257 bp
  20% of sequence in fragments shorter than     316 bp
  25% of sequence in fragments shorter than     369 bp
  30% of sequence in fragments shorter than     429 bp
  35% of sequence in fragments shorter than     489 bp
  40% of sequence in fragments shorter than     550 bp
  45% of sequence in fragments shorter than     611 bp
  50% of sequence in fragments shorter than     678 bp
  55% of sequence in fragments shorter than     748 bp
  60% of sequence in fragments shorter than     832 bp
  65% of sequence in fragments shorter than     922 bp
  70% of sequence in fragments shorter than    1026 bp
  75% of sequence in fragments shorter than    1142 bp
  80% of sequence in fragments shorter than    1281 bp
  85% of sequence in fragments shorter than    1466 bp
  90% of sequence in fragments shorter than    1723 bp
  95% of sequence in fragments shorter than    2156 bp

# Hin4II.XbaI (CCTTC+TCTAGA) double digest, 7910 thousand fragments (7059915+850998 sites), 376 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     266 bp
  20% of sequence in fragments shorter than     327 bp
  25% of sequence in fragments shorter than     381 bp
  30% of sequence in fragments shorter than     443 bp
  35% of sequence in fragments shorter than     506 bp
  40% of sequence in fragments shorter than     566 bp
  45% of sequence in fragments shorter than     636 bp
  50% of sequence in fragments shorter than     706 bp
  55% of sequence in fragments shorter than     785 bp
  60% of sequence in fragments shorter than     871 bp
  65% of sequence in fragments shorter than     967 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1199 bp
  80% of sequence in fragments shorter than    1352 bp
  85% of sequence in fragments shorter than    1547 bp
  90% of sequence in fragments shorter than    1819 bp
  95% of sequence in fragments shorter than    2278 bp

# EcoRI.Hin4II (GAATTC+CCTTC) double digest, 7906 thousand fragments (847401+7059915 sites), 376 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     327 bp
  25% of sequence in fragments shorter than     385 bp
  30% of sequence in fragments shorter than     446 bp
  35% of sequence in fragments shorter than     509 bp
  40% of sequence in fragments shorter than     571 bp
  45% of sequence in fragments shorter than     641 bp
  50% of sequence in fragments shorter than     713 bp
  55% of sequence in fragments shorter than     792 bp
  60% of sequence in fragments shorter than     877 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1081 bp
  75% of sequence in fragments shorter than    1209 bp
  80% of sequence in fragments shorter than    1364 bp
  85% of sequence in fragments shorter than    1558 bp
  90% of sequence in fragments shorter than    1831 bp
  95% of sequence in fragments shorter than    2294 bp

# BceAI.BspKT6I (ACGGC+GATC) double digest, 7904 thousand fragments (705178+7199434 sites), 377 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     302 bp
  25% of sequence in fragments shorter than     354 bp
  30% of sequence in fragments shorter than     409 bp
  35% of sequence in fragments shorter than     462 bp
  40% of sequence in fragments shorter than     517 bp
  45% of sequence in fragments shorter than     578 bp
  50% of sequence in fragments shorter than     641 bp
  55% of sequence in fragments shorter than     709 bp
  60% of sequence in fragments shorter than     784 bp
  65% of sequence in fragments shorter than     865 bp
  70% of sequence in fragments shorter than     961 bp
  75% of sequence in fragments shorter than    1066 bp
  80% of sequence in fragments shorter than    1194 bp
  85% of sequence in fragments shorter than    1364 bp
  90% of sequence in fragments shorter than    1595 bp
  95% of sequence in fragments shorter than    2007 bp

# BciVI.BspKT6I (GTATCC+GATC) double digest, 7895 thousand fragments (696093+7199434 sites), 377 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     182 bp
  15% of sequence in fragments shorter than     245 bp
  20% of sequence in fragments shorter than     302 bp
  25% of sequence in fragments shorter than     352 bp
  30% of sequence in fragments shorter than     405 bp
  35% of sequence in fragments shorter than     459 bp
  40% of sequence in fragments shorter than     511 bp
  45% of sequence in fragments shorter than     568 bp
  50% of sequence in fragments shorter than     630 bp
  55% of sequence in fragments shorter than     696 bp
  60% of sequence in fragments shorter than     770 bp
  65% of sequence in fragments shorter than     851 bp
  70% of sequence in fragments shorter than     942 bp
  75% of sequence in fragments shorter than    1046 bp
  80% of sequence in fragments shorter than    1175 bp
  85% of sequence in fragments shorter than    1341 bp
  90% of sequence in fragments shorter than    1573 bp
  95% of sequence in fragments shorter than    1983 bp

# BspNCI.SacI (CCAGA+GAGCTC) double digest, 7885 thousand fragments (7282436+603323 sites), 377 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     373 bp
  30% of sequence in fragments shorter than     432 bp
  35% of sequence in fragments shorter than     493 bp
  40% of sequence in fragments shorter than     552 bp
  45% of sequence in fragments shorter than     613 bp
  50% of sequence in fragments shorter than     683 bp
  55% of sequence in fragments shorter than     756 bp
  60% of sequence in fragments shorter than     840 bp
  65% of sequence in fragments shorter than     931 bp
  70% of sequence in fragments shorter than    1034 bp
  75% of sequence in fragments shorter than    1146 bp
  80% of sequence in fragments shorter than    1290 bp
  85% of sequence in fragments shorter than    1479 bp
  90% of sequence in fragments shorter than    1744 bp
  95% of sequence in fragments shorter than    2212 bp

# BccI.BfiI (CCATC+ACTGGG) double digest, 7877 thousand fragments (6170927+1706594 sites), 378 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     367 bp
  30% of sequence in fragments shorter than     424 bp
  35% of sequence in fragments shorter than     482 bp
  40% of sequence in fragments shorter than     545 bp
  45% of sequence in fragments shorter than     612 bp
  50% of sequence in fragments shorter than     678 bp
  55% of sequence in fragments shorter than     754 bp
  60% of sequence in fragments shorter than     839 bp
  65% of sequence in fragments shorter than     932 bp
  70% of sequence in fragments shorter than    1039 bp
  75% of sequence in fragments shorter than    1164 bp
  80% of sequence in fragments shorter than    1316 bp
  85% of sequence in fragments shorter than    1518 bp
  90% of sequence in fragments shorter than    1803 bp
  95% of sequence in fragments shorter than    2337 bp

# AvrII.BspNCI (CCTAGG+CCAGA) double digest, 7876 thousand fragments (594956+7282436 sites), 378 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     257 bp
  20% of sequence in fragments shorter than     314 bp
  25% of sequence in fragments shorter than     369 bp
  30% of sequence in fragments shorter than     427 bp
  35% of sequence in fragments shorter than     487 bp
  40% of sequence in fragments shorter than     548 bp
  45% of sequence in fragments shorter than     608 bp
  50% of sequence in fragments shorter than     673 bp
  55% of sequence in fragments shorter than     744 bp
  60% of sequence in fragments shorter than     826 bp
  65% of sequence in fragments shorter than     913 bp
  70% of sequence in fragments shorter than    1015 bp
  75% of sequence in fragments shorter than    1130 bp
  80% of sequence in fragments shorter than    1273 bp
  85% of sequence in fragments shorter than    1456 bp
  90% of sequence in fragments shorter than    1720 bp
  95% of sequence in fragments shorter than    2178 bp

# AflIII.ApoI (ACRYGT+RAATTY) double digest, 7867 thousand fragments (1485394+6382433 sites), 378 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     253 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     370 bp
  30% of sequence in fragments shorter than     426 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     553 bp
  45% of sequence in fragments shorter than     621 bp
  50% of sequence in fragments shorter than     693 bp
  55% of sequence in fragments shorter than     773 bp
  60% of sequence in fragments shorter than     859 bp
  65% of sequence in fragments shorter than     958 bp
  70% of sequence in fragments shorter than    1076 bp
  75% of sequence in fragments shorter than    1214 bp
  80% of sequence in fragments shorter than    1378 bp
  85% of sequence in fragments shorter than    1599 bp
  90% of sequence in fragments shorter than    1939 bp
  95% of sequence in fragments shorter than    2578 bp

# AvaII.CviQI (GGWCC+GTAC) double digest, 7862 thousand fragments (2747690+5115078 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     259 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     425 bp
  35% of sequence in fragments shorter than     483 bp
  40% of sequence in fragments shorter than     540 bp
  45% of sequence in fragments shorter than     601 bp
  50% of sequence in fragments shorter than     664 bp
  55% of sequence in fragments shorter than     732 bp
  60% of sequence in fragments shorter than     807 bp
  65% of sequence in fragments shorter than     889 bp
  70% of sequence in fragments shorter than     982 bp
  75% of sequence in fragments shorter than    1089 bp
  80% of sequence in fragments shorter than    1219 bp
  85% of sequence in fragments shorter than    1373 bp
  90% of sequence in fragments shorter than    1599 bp
  95% of sequence in fragments shorter than    1993 bp

# BscGI.BsmAI (CCCGT+GTCTC) double digest, 7862 thousand fragments (1231070+6631585 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     259 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     384 bp
  30% of sequence in fragments shorter than     453 bp
  35% of sequence in fragments shorter than     521 bp
  40% of sequence in fragments shorter than     590 bp
  45% of sequence in fragments shorter than     666 bp
  50% of sequence in fragments shorter than     746 bp
  55% of sequence in fragments shorter than     835 bp
  60% of sequence in fragments shorter than     932 bp
  65% of sequence in fragments shorter than    1043 bp
  70% of sequence in fragments shorter than    1170 bp
  75% of sequence in fragments shorter than    1320 bp
  80% of sequence in fragments shorter than    1500 bp
  85% of sequence in fragments shorter than    1734 bp
  90% of sequence in fragments shorter than    2062 bp
  95% of sequence in fragments shorter than    2607 bp

# AciI.BscAI (CCGC+GCATC) double digest, 7858 thousand fragments (4206130+3652926 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     125 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     281 bp
  20% of sequence in fragments shorter than     354 bp
  25% of sequence in fragments shorter than     427 bp
  30% of sequence in fragments shorter than     502 bp
  35% of sequence in fragments shorter than     577 bp
  40% of sequence in fragments shorter than     654 bp
  45% of sequence in fragments shorter than     737 bp
  50% of sequence in fragments shorter than     823 bp
  55% of sequence in fragments shorter than     918 bp
  60% of sequence in fragments shorter than    1023 bp
  65% of sequence in fragments shorter than    1138 bp
  70% of sequence in fragments shorter than    1268 bp
  75% of sequence in fragments shorter than    1419 bp
  80% of sequence in fragments shorter than    1604 bp
  85% of sequence in fragments shorter than    1845 bp
  90% of sequence in fragments shorter than    2185 bp
  95% of sequence in fragments shorter than    2764 bp

# BthCI.PsiI (GCNGC+TTATAA) double digest, 7857 thousand fragments (6209929+1647912 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     345 bp
  25% of sequence in fragments shorter than     414 bp
  30% of sequence in fragments shorter than     482 bp
  35% of sequence in fragments shorter than     552 bp
  40% of sequence in fragments shorter than     625 bp
  45% of sequence in fragments shorter than     700 bp
  50% of sequence in fragments shorter than     782 bp
  55% of sequence in fragments shorter than     865 bp
  60% of sequence in fragments shorter than     960 bp
  65% of sequence in fragments shorter than    1059 bp
  70% of sequence in fragments shorter than    1176 bp
  75% of sequence in fragments shorter than    1312 bp
  80% of sequence in fragments shorter than    1476 bp
  85% of sequence in fragments shorter than    1678 bp
  90% of sequence in fragments shorter than    1967 bp
  95% of sequence in fragments shorter than    2467 bp

# BspNCI.HgaI (CCAGA+GACGC) double digest, 7853 thousand fragments (7282436+571889 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     318 bp
  25% of sequence in fragments shorter than     374 bp
  30% of sequence in fragments shorter than     434 bp
  35% of sequence in fragments shorter than     495 bp
  40% of sequence in fragments shorter than     558 bp
  45% of sequence in fragments shorter than     620 bp
  50% of sequence in fragments shorter than     690 bp
  55% of sequence in fragments shorter than     764 bp
  60% of sequence in fragments shorter than     850 bp
  65% of sequence in fragments shorter than     941 bp
  70% of sequence in fragments shorter than    1046 bp
  75% of sequence in fragments shorter than    1161 bp
  80% of sequence in fragments shorter than    1309 bp
  85% of sequence in fragments shorter than    1500 bp
  90% of sequence in fragments shorter than    1768 bp
  95% of sequence in fragments shorter than    2239 bp

# BspNCI.EciI (CCAGA+GGCGGA) double digest, 7853 thousand fragments (7282436+571273 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     129 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     256 bp
  20% of sequence in fragments shorter than     314 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     426 bp
  35% of sequence in fragments shorter than     484 bp
  40% of sequence in fragments shorter than     544 bp
  45% of sequence in fragments shorter than     603 bp
  50% of sequence in fragments shorter than     670 bp
  55% of sequence in fragments shorter than     740 bp
  60% of sequence in fragments shorter than     821 bp
  65% of sequence in fragments shorter than     909 bp
  70% of sequence in fragments shorter than    1012 bp
  75% of sequence in fragments shorter than    1128 bp
  80% of sequence in fragments shorter than    1262 bp
  85% of sequence in fragments shorter than    1445 bp
  90% of sequence in fragments shorter than    1708 bp
  95% of sequence in fragments shorter than    2167 bp

# BspNCI.MfeI (CCAGA+CAATTG) double digest, 7846 thousand fragments (7282436+564303 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     316 bp
  25% of sequence in fragments shorter than     371 bp
  30% of sequence in fragments shorter than     430 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     548 bp
  45% of sequence in fragments shorter than     608 bp
  50% of sequence in fragments shorter than     673 bp
  55% of sequence in fragments shorter than     744 bp
  60% of sequence in fragments shorter than     824 bp
  65% of sequence in fragments shorter than     905 bp
  70% of sequence in fragments shorter than    1006 bp
  75% of sequence in fragments shorter than    1116 bp
  80% of sequence in fragments shorter than    1254 bp
  85% of sequence in fragments shorter than    1431 bp
  90% of sequence in fragments shorter than    1683 bp
  95% of sequence in fragments shorter than    2114 bp

# CviQI.SmlI (GTAC+CTYRAG) double digest, 7842 thousand fragments (5115078+2727867 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     133 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     315 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     423 bp
  35% of sequence in fragments shorter than     478 bp
  40% of sequence in fragments shorter than     534 bp
  45% of sequence in fragments shorter than     593 bp
  50% of sequence in fragments shorter than     656 bp
  55% of sequence in fragments shorter than     718 bp
  60% of sequence in fragments shorter than     792 bp
  65% of sequence in fragments shorter than     871 bp
  70% of sequence in fragments shorter than     960 bp
  75% of sequence in fragments shorter than    1065 bp
  80% of sequence in fragments shorter than    1194 bp
  85% of sequence in fragments shorter than    1353 bp
  90% of sequence in fragments shorter than    1569 bp
  95% of sequence in fragments shorter than    1977 bp

# AflII.BspKT6I (CTTAAG+GATC) double digest, 7842 thousand fragments (643284+7199434 sites), 379 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     305 bp
  25% of sequence in fragments shorter than     356 bp
  30% of sequence in fragments shorter than     411 bp
  35% of sequence in fragments shorter than     465 bp
  40% of sequence in fragments shorter than     518 bp
  45% of sequence in fragments shorter than     576 bp
  50% of sequence in fragments shorter than     637 bp
  55% of sequence in fragments shorter than     704 bp
  60% of sequence in fragments shorter than     779 bp
  65% of sequence in fragments shorter than     862 bp
  70% of sequence in fragments shorter than     953 bp
  75% of sequence in fragments shorter than    1058 bp
  80% of sequence in fragments shorter than    1188 bp
  85% of sequence in fragments shorter than    1358 bp
  90% of sequence in fragments shorter than    1592 bp
  95% of sequence in fragments shorter than    2017 bp

# BglII.Hin4II (AGATCT+CCTTC) double digest, 7834 thousand fragments (774732+7059915 sites), 380 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     388 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     514 bp
  40% of sequence in fragments shorter than     576 bp
  45% of sequence in fragments shorter than     645 bp
  50% of sequence in fragments shorter than     719 bp
  55% of sequence in fragments shorter than     800 bp
  60% of sequence in fragments shorter than     886 bp
  65% of sequence in fragments shorter than     985 bp
  70% of sequence in fragments shorter than    1091 bp
  75% of sequence in fragments shorter than    1220 bp
  80% of sequence in fragments shorter than    1377 bp
  85% of sequence in fragments shorter than    1571 bp
  90% of sequence in fragments shorter than    1847 bp
  95% of sequence in fragments shorter than    2314 bp

# BspNCI.SphI (CCAGA+GCATGC) double digest, 7832 thousand fragments (7282436+550952 sites), 380 bp average fragment length
   5% of sequence in fragments shorter than     127 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     372 bp
  30% of sequence in fragments shorter than     430 bp
  35% of sequence in fragments shorter than     489 bp
  40% of sequence in fragments shorter than     550 bp
  45% of sequence in fragments shorter than     610 bp
  50% of sequence in fragments shorter than     677 bp
  55% of sequence in fragments shorter than     748 bp
  60% of sequence in fragments shorter than     830 bp
  65% of sequence in fragments shorter than     919 bp
  70% of sequence in fragments shorter than    1022 bp
  75% of sequence in fragments shorter than    1137 bp
  80% of sequence in fragments shorter than    1272 bp
  85% of sequence in fragments shorter than    1455 bp
  90% of sequence in fragments shorter than    1716 bp
  95% of sequence in fragments shorter than    2168 bp

# BspKT6I.HaeII (GATC+RGCGCY) double digest, 7829 thousand fragments (7199434+630101 sites), 380 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     249 bp
  20% of sequence in fragments shorter than     307 bp
  25% of sequence in fragments shorter than     357 bp
  30% of sequence in fragments shorter than     413 bp
  35% of sequence in fragments shorter than     468 bp
  40% of sequence in fragments shorter than     524 bp
  45% of sequence in fragments shorter than     586 bp
  50% of sequence in fragments shorter than     650 bp
  55% of sequence in fragments shorter than     717 bp
  60% of sequence in fragments shorter than     793 bp
  65% of sequence in fragments shorter than     876 bp
  70% of sequence in fragments shorter than     972 bp
  75% of sequence in fragments shorter than    1075 bp
  80% of sequence in fragments shorter than    1205 bp
  85% of sequence in fragments shorter than    1372 bp
  90% of sequence in fragments shorter than    1600 bp
  95% of sequence in fragments shorter than    1997 bp

# BspNCI.ScaI (CCAGA+AGTACT) double digest, 7825 thousand fragments (7282436+543793 sites), 380 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     259 bp
  20% of sequence in fragments shorter than     318 bp
  25% of sequence in fragments shorter than     373 bp
  30% of sequence in fragments shorter than     431 bp
  35% of sequence in fragments shorter than     490 bp
  40% of sequence in fragments shorter than     550 bp
  45% of sequence in fragments shorter than     609 bp
  50% of sequence in fragments shorter than     675 bp
  55% of sequence in fragments shorter than     745 bp
  60% of sequence in fragments shorter than     826 bp
  65% of sequence in fragments shorter than     913 bp
  70% of sequence in fragments shorter than    1014 bp
  75% of sequence in fragments shorter than    1128 bp
  80% of sequence in fragments shorter than    1259 bp
  85% of sequence in fragments shorter than    1437 bp
  90% of sequence in fragments shorter than    1691 bp
  95% of sequence in fragments shorter than    2135 bp

# ApoI.FauI (RAATTY+CCCGC) double digest, 7821 thousand fragments (6382433+1439772 sites), 380 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     199 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     316 bp
  25% of sequence in fragments shorter than     373 bp
  30% of sequence in fragments shorter than     432 bp
  35% of sequence in fragments shorter than     493 bp
  40% of sequence in fragments shorter than     556 bp
  45% of sequence in fragments shorter than     619 bp
  50% of sequence in fragments shorter than     689 bp
  55% of sequence in fragments shorter than     761 bp
  60% of sequence in fragments shorter than     844 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1032 bp
  75% of sequence in fragments shorter than    1152 bp
  80% of sequence in fragments shorter than    1296 bp
  85% of sequence in fragments shorter than    1471 bp
  90% of sequence in fragments shorter than    1732 bp
  95% of sequence in fragments shorter than    2174 bp

# ApoI.BspMI (RAATTY+ACCTGC) double digest, 7820 thousand fragments (6382433+1438129 sites), 381 bp average fragment length
   5% of sequence in fragments shorter than     129 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     255 bp
  20% of sequence in fragments shorter than     313 bp
  25% of sequence in fragments shorter than     366 bp
  30% of sequence in fragments shorter than     423 bp
  35% of sequence in fragments shorter than     483 bp
  40% of sequence in fragments shorter than     545 bp
  45% of sequence in fragments shorter than     607 bp
  50% of sequence in fragments shorter than     675 bp
  55% of sequence in fragments shorter than     746 bp
  60% of sequence in fragments shorter than     822 bp
  65% of sequence in fragments shorter than     902 bp
  70% of sequence in fragments shorter than    1000 bp
  75% of sequence in fragments shorter than    1115 bp
  80% of sequence in fragments shorter than    1257 bp
  85% of sequence in fragments shorter than    1420 bp
  90% of sequence in fragments shorter than    1669 bp
  95% of sequence in fragments shorter than    2097 bp

# Hin4II.NcoI (CCTTC+CCATGG) double digest, 7819 thousand fragments (7059915+759594 sites), 381 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     269 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     451 bp
  35% of sequence in fragments shorter than     515 bp
  40% of sequence in fragments shorter than     579 bp
  45% of sequence in fragments shorter than     652 bp
  50% of sequence in fragments shorter than     728 bp
  55% of sequence in fragments shorter than     811 bp
  60% of sequence in fragments shorter than     896 bp
  65% of sequence in fragments shorter than     998 bp
  70% of sequence in fragments shorter than    1110 bp
  75% of sequence in fragments shorter than    1241 bp
  80% of sequence in fragments shorter than    1402 bp
  85% of sequence in fragments shorter than    1611 bp
  90% of sequence in fragments shorter than    1895 bp
  95% of sequence in fragments shorter than    2388 bp

# BccI.PsiI (CCATC+TTATAA) double digest, 7818 thousand fragments (6170927+1647912 sites), 381 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     314 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     423 bp
  35% of sequence in fragments shorter than     477 bp
  40% of sequence in fragments shorter than     535 bp
  45% of sequence in fragments shorter than     595 bp
  50% of sequence in fragments shorter than     657 bp
  55% of sequence in fragments shorter than     725 bp
  60% of sequence in fragments shorter than     799 bp
  65% of sequence in fragments shorter than     881 bp
  70% of sequence in fragments shorter than     975 bp
  75% of sequence in fragments shorter than    1083 bp
  80% of sequence in fragments shorter than    1213 bp
  85% of sequence in fragments shorter than    1381 bp
  90% of sequence in fragments shorter than    1625 bp
  95% of sequence in fragments shorter than    2074 bp

# BthCI.EsaBC3I (GCNGC+TCGA) double digest, 7812 thousand fragments (6209929+1603346 sites), 381 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     281 bp
  20% of sequence in fragments shorter than     348 bp
  25% of sequence in fragments shorter than     419 bp
  30% of sequence in fragments shorter than     493 bp
  35% of sequence in fragments shorter than     568 bp
  40% of sequence in fragments shorter than     647 bp
  45% of sequence in fragments shorter than     731 bp
  50% of sequence in fragments shorter than     822 bp
  55% of sequence in fragments shorter than     920 bp
  60% of sequence in fragments shorter than    1030 bp
  65% of sequence in fragments shorter than    1146 bp
  70% of sequence in fragments shorter than    1284 bp
  75% of sequence in fragments shorter than    1445 bp
  80% of sequence in fragments shorter than    1635 bp
  85% of sequence in fragments shorter than    1880 bp
  90% of sequence in fragments shorter than    2221 bp
  95% of sequence in fragments shorter than    2818 bp

# BspKT6I.SacI (GATC+GAGCTC) double digest, 7802 thousand fragments (7199434+603323 sites), 381 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     183 bp
  15% of sequence in fragments shorter than     248 bp
  20% of sequence in fragments shorter than     305 bp
  25% of sequence in fragments shorter than     358 bp
  30% of sequence in fragments shorter than     414 bp
  35% of sequence in fragments shorter than     467 bp
  40% of sequence in fragments shorter than     523 bp
  45% of sequence in fragments shorter than     584 bp
  50% of sequence in fragments shorter than     648 bp
  55% of sequence in fragments shorter than     716 bp
  60% of sequence in fragments shorter than     792 bp
  65% of sequence in fragments shorter than     875 bp
  70% of sequence in fragments shorter than     970 bp
  75% of sequence in fragments shorter than    1074 bp
  80% of sequence in fragments shorter than    1206 bp
  85% of sequence in fragments shorter than    1378 bp
  90% of sequence in fragments shorter than    1617 bp
  95% of sequence in fragments shorter than    2042 bp

# BclI.Hin4II (TGATCA+CCTTC) double digest, 7798 thousand fragments (738785+7059915 sites), 382 bp average fragment length
   5% of sequence in fragments shorter than     133 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     332 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     513 bp
  40% of sequence in fragments shorter than     574 bp
  45% of sequence in fragments shorter than     643 bp
  50% of sequence in fragments shorter than     716 bp
  55% of sequence in fragments shorter than     796 bp
  60% of sequence in fragments shorter than     884 bp
  65% of sequence in fragments shorter than     981 bp
  70% of sequence in fragments shorter than    1086 bp
  75% of sequence in fragments shorter than    1214 bp
  80% of sequence in fragments shorter than    1369 bp
  85% of sequence in fragments shorter than    1562 bp
  90% of sequence in fragments shorter than    1834 bp
  95% of sequence in fragments shorter than    2295 bp

# AvrII.BspKT6I (CCTAGG+GATC) double digest, 7793 thousand fragments (594956+7199434 sites), 382 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     250 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     361 bp
  30% of sequence in fragments shorter than     415 bp
  35% of sequence in fragments shorter than     468 bp
  40% of sequence in fragments shorter than     524 bp
  45% of sequence in fragments shorter than     585 bp
  50% of sequence in fragments shorter than     647 bp
  55% of sequence in fragments shorter than     716 bp
  60% of sequence in fragments shorter than     793 bp
  65% of sequence in fragments shorter than     876 bp
  70% of sequence in fragments shorter than     971 bp
  75% of sequence in fragments shorter than    1079 bp
  80% of sequence in fragments shorter than    1211 bp
  85% of sequence in fragments shorter than    1383 bp
  90% of sequence in fragments shorter than    1627 bp
  95% of sequence in fragments shorter than    2063 bp

# FnuDII.Hin4II (CGCG+CCTTC) double digest, 7793 thousand fragments (733940+7059915 sites), 382 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     275 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     463 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     596 bp
  45% of sequence in fragments shorter than     669 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     833 bp
  60% of sequence in fragments shorter than     924 bp
  65% of sequence in fragments shorter than    1026 bp
  70% of sequence in fragments shorter than    1142 bp
  75% of sequence in fragments shorter than    1279 bp
  80% of sequence in fragments shorter than    1442 bp
  85% of sequence in fragments shorter than    1655 bp
  90% of sequence in fragments shorter than    1944 bp
  95% of sequence in fragments shorter than    2440 bp

# ApaLI.BspNCI (GTGCAC+CCAGA) double digest, 7778 thousand fragments (496255+7282436 sites), 383 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     260 bp
  20% of sequence in fragments shorter than     320 bp
  25% of sequence in fragments shorter than     376 bp
  30% of sequence in fragments shorter than     434 bp
  35% of sequence in fragments shorter than     494 bp
  40% of sequence in fragments shorter than     556 bp
  45% of sequence in fragments shorter than     616 bp
  50% of sequence in fragments shorter than     685 bp
  55% of sequence in fragments shorter than     757 bp
  60% of sequence in fragments shorter than     840 bp
  65% of sequence in fragments shorter than     926 bp
  70% of sequence in fragments shorter than    1031 bp
  75% of sequence in fragments shorter than    1145 bp
  80% of sequence in fragments shorter than    1283 bp
  85% of sequence in fragments shorter than    1469 bp
  90% of sequence in fragments shorter than    1731 bp
  95% of sequence in fragments shorter than    2188 bp

# BccI.EsaBC3I (CCATC+TCGA) double digest, 7773 thousand fragments (6170927+1603346 sites), 383 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     323 bp
  25% of sequence in fragments shorter than     381 bp
  30% of sequence in fragments shorter than     439 bp
  35% of sequence in fragments shorter than     498 bp
  40% of sequence in fragments shorter than     561 bp
  45% of sequence in fragments shorter than     626 bp
  50% of sequence in fragments shorter than     696 bp
  55% of sequence in fragments shorter than     772 bp
  60% of sequence in fragments shorter than     856 bp
  65% of sequence in fragments shorter than     949 bp
  70% of sequence in fragments shorter than    1056 bp
  75% of sequence in fragments shorter than    1174 bp
  80% of sequence in fragments shorter than    1323 bp
  85% of sequence in fragments shorter than    1513 bp
  90% of sequence in fragments shorter than    1788 bp
  95% of sequence in fragments shorter than    2282 bp

# BsaAI.Hin4II (YACGTR+CCTTC) double digest, 7773 thousand fragments (713801+7059915 sites), 383 bp average fragment length
   5% of sequence in fragments shorter than     133 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     272 bp
  20% of sequence in fragments shorter than     333 bp
  25% of sequence in fragments shorter than     390 bp
  30% of sequence in fragments shorter than     452 bp
  35% of sequence in fragments shorter than     517 bp
  40% of sequence in fragments shorter than     580 bp
  45% of sequence in fragments shorter than     651 bp
  50% of sequence in fragments shorter than     727 bp
  55% of sequence in fragments shorter than     806 bp
  60% of sequence in fragments shorter than     893 bp
  65% of sequence in fragments shorter than     993 bp
  70% of sequence in fragments shorter than    1102 bp
  75% of sequence in fragments shorter than    1232 bp
  80% of sequence in fragments shorter than    1389 bp
  85% of sequence in fragments shorter than    1592 bp
  90% of sequence in fragments shorter than    1872 bp
  95% of sequence in fragments shorter than    2349 bp

# BspKT6I.HgaI (GATC+GACGC) double digest, 7770 thousand fragments (7199434+571889 sites), 383 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     184 bp
  15% of sequence in fragments shorter than     250 bp
  20% of sequence in fragments shorter than     307 bp
  25% of sequence in fragments shorter than     360 bp
  30% of sequence in fragments shorter than     416 bp
  35% of sequence in fragments shorter than     470 bp
  40% of sequence in fragments shorter than     527 bp
  45% of sequence in fragments shorter than     588 bp
  50% of sequence in fragments shorter than     652 bp
  55% of sequence in fragments shorter than     722 bp
  60% of sequence in fragments shorter than     799 bp
  65% of sequence in fragments shorter than     883 bp
  70% of sequence in fragments shorter than     978 bp
  75% of sequence in fragments shorter than    1082 bp
  80% of sequence in fragments shorter than    1215 bp
  85% of sequence in fragments shorter than    1386 bp
  90% of sequence in fragments shorter than    1632 bp
  95% of sequence in fragments shorter than    2060 bp

# BspKT6I.EciI (GATC+GGCGGA) double digest, 7770 thousand fragments (7199434+571273 sites), 383 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     255 bp
  20% of sequence in fragments shorter than     316 bp
  25% of sequence in fragments shorter than     372 bp
  30% of sequence in fragments shorter than     429 bp
  35% of sequence in fragments shorter than     485 bp
  40% of sequence in fragments shorter than     546 bp
  45% of sequence in fragments shorter than     610 bp
  50% of sequence in fragments shorter than     677 bp
  55% of sequence in fragments shorter than     750 bp
  60% of sequence in fragments shorter than     830 bp
  65% of sequence in fragments shorter than     918 bp
  70% of sequence in fragments shorter than    1018 bp
  75% of sequence in fragments shorter than    1126 bp
  80% of sequence in fragments shorter than    1265 bp
  85% of sequence in fragments shorter than    1441 bp
  90% of sequence in fragments shorter than    1697 bp
  95% of sequence in fragments shorter than    2136 bp

# BceAI.Hin4II (ACGGC+CCTTC) double digest, 7764 thousand fragments (705178+7059915 sites), 383 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     272 bp
  20% of sequence in fragments shorter than     335 bp
  25% of sequence in fragments shorter than     394 bp
  30% of sequence in fragments shorter than     457 bp
  35% of sequence in fragments shorter than     523 bp
  40% of sequence in fragments shorter than     588 bp
  45% of sequence in fragments shorter than     661 bp
  50% of sequence in fragments shorter than     739 bp
  55% of sequence in fragments shorter than     824 bp
  60% of sequence in fragments shorter than     915 bp
  65% of sequence in fragments shorter than    1017 bp
  70% of sequence in fragments shorter than    1132 bp
  75% of sequence in fragments shorter than    1270 bp
  80% of sequence in fragments shorter than    1433 bp
  85% of sequence in fragments shorter than    1646 bp
  90% of sequence in fragments shorter than    1937 bp
  95% of sequence in fragments shorter than    2435 bp

# HhaI.HphI (GCGC+GGTGA) double digest, 7763 thousand fragments (1756502+6007336 sites), 383 bp average fragment length
   5% of sequence in fragments shorter than     122 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     330 bp
  25% of sequence in fragments shorter than     390 bp
  30% of sequence in fragments shorter than     456 bp
  35% of sequence in fragments shorter than     523 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     665 bp
  50% of sequence in fragments shorter than     741 bp
  55% of sequence in fragments shorter than     823 bp
  60% of sequence in fragments shorter than     917 bp
  65% of sequence in fragments shorter than    1023 bp
  70% of sequence in fragments shorter than    1141 bp
  75% of sequence in fragments shorter than    1280 bp
  80% of sequence in fragments shorter than    1438 bp
  85% of sequence in fragments shorter than    1656 bp
  90% of sequence in fragments shorter than    1957 bp
  95% of sequence in fragments shorter than    2466 bp

# BspKT6I.MfeI (GATC+CAATTG) double digest, 7763 thousand fragments (7199434+564303 sites), 383 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     186 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     360 bp
  30% of sequence in fragments shorter than     415 bp
  35% of sequence in fragments shorter than     468 bp
  40% of sequence in fragments shorter than     522 bp
  45% of sequence in fragments shorter than     583 bp
  50% of sequence in fragments shorter than     643 bp
  55% of sequence in fragments shorter than     711 bp
  60% of sequence in fragments shorter than     785 bp
  65% of sequence in fragments shorter than     866 bp
  70% of sequence in fragments shorter than     960 bp
  75% of sequence in fragments shorter than    1067 bp
  80% of sequence in fragments shorter than    1195 bp
  85% of sequence in fragments shorter than    1362 bp
  90% of sequence in fragments shorter than    1594 bp
  95% of sequence in fragments shorter than    1998 bp

# BthCI.HgiCI (GCNGC+GGYRCC) double digest, 7760 thousand fragments (6209929+1550877 sites), 384 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     286 bp
  20% of sequence in fragments shorter than     357 bp
  25% of sequence in fragments shorter than     435 bp
  30% of sequence in fragments shorter than     516 bp
  35% of sequence in fragments shorter than     597 bp
  40% of sequence in fragments shorter than     682 bp
  45% of sequence in fragments shorter than     774 bp
  50% of sequence in fragments shorter than     867 bp
  55% of sequence in fragments shorter than     975 bp
  60% of sequence in fragments shorter than    1085 bp
  65% of sequence in fragments shorter than    1219 bp
  70% of sequence in fragments shorter than    1371 bp
  75% of sequence in fragments shorter than    1549 bp
  80% of sequence in fragments shorter than    1759 bp
  85% of sequence in fragments shorter than    2036 bp
  90% of sequence in fragments shorter than    2434 bp
  95% of sequence in fragments shorter than    3115 bp

# BsmI.BthCI (GAATGC+GCNGC) double digest, 7758 thousand fragments (1549350+6209929 sites), 384 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     349 bp
  25% of sequence in fragments shorter than     428 bp
  30% of sequence in fragments shorter than     508 bp
  35% of sequence in fragments shorter than     589 bp
  40% of sequence in fragments shorter than     676 bp
  45% of sequence in fragments shorter than     767 bp
  50% of sequence in fragments shorter than     863 bp
  55% of sequence in fragments shorter than     967 bp
  60% of sequence in fragments shorter than    1076 bp
  65% of sequence in fragments shorter than    1204 bp
  70% of sequence in fragments shorter than    1348 bp
  75% of sequence in fragments shorter than    1512 bp
  80% of sequence in fragments shorter than    1715 bp
  85% of sequence in fragments shorter than    1971 bp
  90% of sequence in fragments shorter than    2319 bp
  95% of sequence in fragments shorter than    2921 bp

# BciVI.Hin4II (GTATCC+CCTTC) double digest, 7755 thousand fragments (696093+7059915 sites), 384 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     272 bp
  20% of sequence in fragments shorter than     335 bp
  25% of sequence in fragments shorter than     392 bp
  30% of sequence in fragments shorter than     454 bp
  35% of sequence in fragments shorter than     519 bp
  40% of sequence in fragments shorter than     584 bp
  45% of sequence in fragments shorter than     654 bp
  50% of sequence in fragments shorter than     729 bp
  55% of sequence in fragments shorter than     810 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than     998 bp
  70% of sequence in fragments shorter than    1104 bp
  75% of sequence in fragments shorter than    1234 bp
  80% of sequence in fragments shorter than    1389 bp
  85% of sequence in fragments shorter than    1589 bp
  90% of sequence in fragments shorter than    1863 bp
  95% of sequence in fragments shorter than    2335 bp

# BspKT6I.SphI (GATC+GCATGC) double digest, 7749 thousand fragments (7199434+550952 sites), 384 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     309 bp
  25% of sequence in fragments shorter than     362 bp
  30% of sequence in fragments shorter than     419 bp
  35% of sequence in fragments shorter than     473 bp
  40% of sequence in fragments shorter than     530 bp
  45% of sequence in fragments shorter than     591 bp
  50% of sequence in fragments shorter than     655 bp
  55% of sequence in fragments shorter than     725 bp
  60% of sequence in fragments shorter than     802 bp
  65% of sequence in fragments shorter than     886 bp
  70% of sequence in fragments shorter than     983 bp
  75% of sequence in fragments shorter than    1086 bp
  80% of sequence in fragments shorter than    1219 bp
  85% of sequence in fragments shorter than    1387 bp
  90% of sequence in fragments shorter than    1632 bp
  95% of sequence in fragments shorter than    2060 bp

# BspKT6I.ScaI (GATC+AGTACT) double digest, 7742 thousand fragments (7199434+543793 sites), 384 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     185 bp
  15% of sequence in fragments shorter than     250 bp
  20% of sequence in fragments shorter than     308 bp
  25% of sequence in fragments shorter than     360 bp
  30% of sequence in fragments shorter than     415 bp
  35% of sequence in fragments shorter than     469 bp
  40% of sequence in fragments shorter than     525 bp
  45% of sequence in fragments shorter than     585 bp
  50% of sequence in fragments shorter than     649 bp
  55% of sequence in fragments shorter than     717 bp
  60% of sequence in fragments shorter than     793 bp
  65% of sequence in fragments shorter than     875 bp
  70% of sequence in fragments shorter than     970 bp
  75% of sequence in fragments shorter than    1074 bp
  80% of sequence in fragments shorter than    1203 bp
  85% of sequence in fragments shorter than    1374 bp
  90% of sequence in fragments shorter than    1610 bp
  95% of sequence in fragments shorter than    2035 bp

# ApaI.BspNCI (GGGCCC+CCAGA) double digest, 7742 thousand fragments (460339+7282436 sites), 384 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     324 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     443 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     632 bp
  50% of sequence in fragments shorter than     704 bp
  55% of sequence in fragments shorter than     781 bp
  60% of sequence in fragments shorter than     866 bp
  65% of sequence in fragments shorter than     963 bp
  70% of sequence in fragments shorter than    1071 bp
  75% of sequence in fragments shorter than    1188 bp
  80% of sequence in fragments shorter than    1341 bp
  85% of sequence in fragments shorter than    1537 bp
  90% of sequence in fragments shorter than    1814 bp
  95% of sequence in fragments shorter than    2301 bp

# AciI.SfeI (CCGC+CTRYAG) double digest, 7736 thousand fragments (4206130+3531009 sites), 385 bp average fragment length
   5% of sequence in fragments shorter than     123 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     441 bp
  30% of sequence in fragments shorter than     515 bp
  35% of sequence in fragments shorter than     594 bp
  40% of sequence in fragments shorter than     675 bp
  45% of sequence in fragments shorter than     762 bp
  50% of sequence in fragments shorter than     856 bp
  55% of sequence in fragments shorter than     955 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1187 bp
  70% of sequence in fragments shorter than    1331 bp
  75% of sequence in fragments shorter than    1490 bp
  80% of sequence in fragments shorter than    1690 bp
  85% of sequence in fragments shorter than    1938 bp
  90% of sequence in fragments shorter than    2286 bp
  95% of sequence in fragments shorter than    2888 bp

# AccI.BsmAI (GTMKAC+GTCTC) double digest, 7732 thousand fragments (1101063+6631585 sites), 385 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     255 bp
  20% of sequence in fragments shorter than     320 bp
  25% of sequence in fragments shorter than     381 bp
  30% of sequence in fragments shorter than     444 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     571 bp
  45% of sequence in fragments shorter than     637 bp
  50% of sequence in fragments shorter than     711 bp
  55% of sequence in fragments shorter than     791 bp
  60% of sequence in fragments shorter than     879 bp
  65% of sequence in fragments shorter than     976 bp
  70% of sequence in fragments shorter than    1085 bp
  75% of sequence in fragments shorter than    1215 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1568 bp
  90% of sequence in fragments shorter than    1823 bp
  95% of sequence in fragments shorter than    2319 bp

# AvaII.TfiI (GGWCC+GAWTC) double digest, 7731 thousand fragments (2747690+4984362 sites), 385 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     319 bp
  25% of sequence in fragments shorter than     364 bp
  30% of sequence in fragments shorter than     421 bp
  35% of sequence in fragments shorter than     481 bp
  40% of sequence in fragments shorter than     542 bp
  45% of sequence in fragments shorter than     607 bp
  50% of sequence in fragments shorter than     672 bp
  55% of sequence in fragments shorter than     741 bp
  60% of sequence in fragments shorter than     817 bp
  65% of sequence in fragments shorter than     903 bp
  70% of sequence in fragments shorter than    1000 bp
  75% of sequence in fragments shorter than    1112 bp
  80% of sequence in fragments shorter than    1248 bp
  85% of sequence in fragments shorter than    1419 bp
  90% of sequence in fragments shorter than    1657 bp
  95% of sequence in fragments shorter than    2057 bp

# BspNCI.EcoRV (CCAGA+GATATC) double digest, 7728 thousand fragments (7282436+446473 sites), 385 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     322 bp
  25% of sequence in fragments shorter than     376 bp
  30% of sequence in fragments shorter than     435 bp
  35% of sequence in fragments shorter than     496 bp
  40% of sequence in fragments shorter than     558 bp
  45% of sequence in fragments shorter than     620 bp
  50% of sequence in fragments shorter than     689 bp
  55% of sequence in fragments shorter than     762 bp
  60% of sequence in fragments shorter than     845 bp
  65% of sequence in fragments shorter than     934 bp
  70% of sequence in fragments shorter than    1038 bp
  75% of sequence in fragments shorter than    1148 bp
  80% of sequence in fragments shorter than    1290 bp
  85% of sequence in fragments shorter than    1474 bp
  90% of sequence in fragments shorter than    1733 bp
  95% of sequence in fragments shorter than    2186 bp

# BccI.HgiCI (CCATC+GGYRCC) double digest, 7721 thousand fragments (6170927+1550877 sites), 385 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     199 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     322 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     443 bp
  35% of sequence in fragments shorter than     507 bp
  40% of sequence in fragments shorter than     574 bp
  45% of sequence in fragments shorter than     644 bp
  50% of sequence in fragments shorter than     718 bp
  55% of sequence in fragments shorter than     800 bp
  60% of sequence in fragments shorter than     889 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1101 bp
  75% of sequence in fragments shorter than    1236 bp
  80% of sequence in fragments shorter than    1402 bp
  85% of sequence in fragments shorter than    1621 bp
  90% of sequence in fragments shorter than    1939 bp
  95% of sequence in fragments shorter than    2542 bp

# BccI.BsmI (CCATC+GAATGC) double digest, 7719 thousand fragments (6170927+1549350 sites), 386 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     259 bp
  20% of sequence in fragments shorter than     318 bp
  25% of sequence in fragments shorter than     375 bp
  30% of sequence in fragments shorter than     435 bp
  35% of sequence in fragments shorter than     495 bp
  40% of sequence in fragments shorter than     558 bp
  45% of sequence in fragments shorter than     626 bp
  50% of sequence in fragments shorter than     696 bp
  55% of sequence in fragments shorter than     771 bp
  60% of sequence in fragments shorter than     853 bp
  65% of sequence in fragments shorter than     945 bp
  70% of sequence in fragments shorter than    1047 bp
  75% of sequence in fragments shorter than    1163 bp
  80% of sequence in fragments shorter than    1308 bp
  85% of sequence in fragments shorter than    1493 bp
  90% of sequence in fragments shorter than    1748 bp
  95% of sequence in fragments shorter than    2189 bp

# BsmAI.PpuMI (GTCTC+RGGWCCY) double digest, 7716 thousand fragments (6631585+1085138 sites), 386 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     190 bp
  15% of sequence in fragments shorter than     251 bp
  20% of sequence in fragments shorter than     318 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     517 bp
  40% of sequence in fragments shorter than     584 bp
  45% of sequence in fragments shorter than     658 bp
  50% of sequence in fragments shorter than     740 bp
  55% of sequence in fragments shorter than     829 bp
  60% of sequence in fragments shorter than     928 bp
  65% of sequence in fragments shorter than    1039 bp
  70% of sequence in fragments shorter than    1165 bp
  75% of sequence in fragments shorter than    1318 bp
  80% of sequence in fragments shorter than    1503 bp
  85% of sequence in fragments shorter than    1750 bp
  90% of sequence in fragments shorter than    2093 bp
  95% of sequence in fragments shorter than    2735 bp

# BsmAI.PvuII (GTCTC+CAGCTG) double digest, 7715 thousand fragments (6631585+1084260 sites), 386 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     191 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     321 bp
  25% of sequence in fragments shorter than     385 bp
  30% of sequence in fragments shorter than     451 bp
  35% of sequence in fragments shorter than     518 bp
  40% of sequence in fragments shorter than     585 bp
  45% of sequence in fragments shorter than     658 bp
  50% of sequence in fragments shorter than     739 bp
  55% of sequence in fragments shorter than     827 bp
  60% of sequence in fragments shorter than     924 bp
  65% of sequence in fragments shorter than    1035 bp
  70% of sequence in fragments shorter than    1160 bp
  75% of sequence in fragments shorter than    1311 bp
  80% of sequence in fragments shorter than    1495 bp
  85% of sequence in fragments shorter than    1742 bp
  90% of sequence in fragments shorter than    2093 bp
  95% of sequence in fragments shorter than    2726 bp

# BfiI.HphI (ACTGGG+GGTGA) double digest, 7713 thousand fragments (1706594+6007336 sites), 386 bp average fragment length
   5% of sequence in fragments shorter than     133 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     259 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     371 bp
  30% of sequence in fragments shorter than     428 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     549 bp
  45% of sequence in fragments shorter than     613 bp
  50% of sequence in fragments shorter than     683 bp
  55% of sequence in fragments shorter than     756 bp
  60% of sequence in fragments shorter than     835 bp
  65% of sequence in fragments shorter than     926 bp
  70% of sequence in fragments shorter than    1029 bp
  75% of sequence in fragments shorter than    1150 bp
  80% of sequence in fragments shorter than    1297 bp
  85% of sequence in fragments shorter than    1475 bp
  90% of sequence in fragments shorter than    1729 bp
  95% of sequence in fragments shorter than    2186 bp

# SmlI.TfiI (CTYRAG+GAWTC) double digest, 7711 thousand fragments (2727867+4984362 sites), 386 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     199 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     316 bp
  25% of sequence in fragments shorter than     359 bp
  30% of sequence in fragments shorter than     415 bp
  35% of sequence in fragments shorter than     472 bp
  40% of sequence in fragments shorter than     528 bp
  45% of sequence in fragments shorter than     590 bp
  50% of sequence in fragments shorter than     655 bp
  55% of sequence in fragments shorter than     721 bp
  60% of sequence in fragments shorter than     795 bp
  65% of sequence in fragments shorter than     874 bp
  70% of sequence in fragments shorter than     967 bp
  75% of sequence in fragments shorter than    1072 bp
  80% of sequence in fragments shorter than    1200 bp
  85% of sequence in fragments shorter than    1362 bp
  90% of sequence in fragments shorter than    1588 bp
  95% of sequence in fragments shorter than    1968 bp

# ApoI.PstI (RAATTY+CTGCAG) double digest, 7703 thousand fragments (6382433+1321469 sites), 386 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     259 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     373 bp
  30% of sequence in fragments shorter than     431 bp
  35% of sequence in fragments shorter than     492 bp
  40% of sequence in fragments shorter than     555 bp
  45% of sequence in fragments shorter than     620 bp
  50% of sequence in fragments shorter than     689 bp
  55% of sequence in fragments shorter than     763 bp
  60% of sequence in fragments shorter than     846 bp
  65% of sequence in fragments shorter than     934 bp
  70% of sequence in fragments shorter than    1035 bp
  75% of sequence in fragments shorter than    1153 bp
  80% of sequence in fragments shorter than    1298 bp
  85% of sequence in fragments shorter than    1469 bp
  90% of sequence in fragments shorter than    1732 bp
  95% of sequence in fragments shorter than    2196 bp

# AflII.Hin4II (CTTAAG+CCTTC) double digest, 7702 thousand fragments (643284+7059915 sites), 386 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     337 bp
  25% of sequence in fragments shorter than     395 bp
  30% of sequence in fragments shorter than     456 bp
  35% of sequence in fragments shorter than     522 bp
  40% of sequence in fragments shorter than     585 bp
  45% of sequence in fragments shorter than     656 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     900 bp
  65% of sequence in fragments shorter than    1000 bp
  70% of sequence in fragments shorter than    1106 bp
  75% of sequence in fragments shorter than    1236 bp
  80% of sequence in fragments shorter than    1392 bp
  85% of sequence in fragments shorter than    1593 bp
  90% of sequence in fragments shorter than    1870 bp
  95% of sequence in fragments shorter than    2345 bp

# ApaLI.BspKT6I (GTGCAC+GATC) double digest, 7695 thousand fragments (496255+7199434 sites), 387 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     187 bp
  15% of sequence in fragments shorter than     253 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     364 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     474 bp
  40% of sequence in fragments shorter than     528 bp
  45% of sequence in fragments shorter than     589 bp
  50% of sequence in fragments shorter than     654 bp
  55% of sequence in fragments shorter than     724 bp
  60% of sequence in fragments shorter than     801 bp
  65% of sequence in fragments shorter than     885 bp
  70% of sequence in fragments shorter than     982 bp
  75% of sequence in fragments shorter than    1085 bp
  80% of sequence in fragments shorter than    1219 bp
  85% of sequence in fragments shorter than    1388 bp
  90% of sequence in fragments shorter than    1633 bp
  95% of sequence in fragments shorter than    2063 bp

# AflIII.BthCI (ACRYGT+GCNGC) double digest, 7694 thousand fragments (1485394+6209929 sites), 387 bp average fragment length
   5% of sequence in fragments shorter than     129 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     289 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     435 bp
  30% of sequence in fragments shorter than     512 bp
  35% of sequence in fragments shorter than     591 bp
  40% of sequence in fragments shorter than     673 bp
  45% of sequence in fragments shorter than     761 bp
  50% of sequence in fragments shorter than     851 bp
  55% of sequence in fragments shorter than     949 bp
  60% of sequence in fragments shorter than    1056 bp
  65% of sequence in fragments shorter than    1178 bp
  70% of sequence in fragments shorter than    1315 bp
  75% of sequence in fragments shorter than    1475 bp
  80% of sequence in fragments shorter than    1664 bp
  85% of sequence in fragments shorter than    1912 bp
  90% of sequence in fragments shorter than    2243 bp
  95% of sequence in fragments shorter than    2843 bp

# HaeII.Hin4II (RGCGCY+CCTTC) double digest, 7689 thousand fragments (630101+7059915 sites), 387 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     338 bp
  25% of sequence in fragments shorter than     396 bp
  30% of sequence in fragments shorter than     459 bp
  35% of sequence in fragments shorter than     524 bp
  40% of sequence in fragments shorter than     589 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     740 bp
  55% of sequence in fragments shorter than     824 bp
  60% of sequence in fragments shorter than     915 bp
  65% of sequence in fragments shorter than    1016 bp
  70% of sequence in fragments shorter than    1133 bp
  75% of sequence in fragments shorter than    1271 bp
  80% of sequence in fragments shorter than    1437 bp
  85% of sequence in fragments shorter than    1648 bp
  90% of sequence in fragments shorter than    1941 bp
  95% of sequence in fragments shorter than    2435 bp

# BspNCI.SpeI (CCAGA+ACTAGT) double digest, 7682 thousand fragments (7282436+400286 sites), 387 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     323 bp
  25% of sequence in fragments shorter than     380 bp
  30% of sequence in fragments shorter than     437 bp
  35% of sequence in fragments shorter than     497 bp
  40% of sequence in fragments shorter than     560 bp
  45% of sequence in fragments shorter than     619 bp
  50% of sequence in fragments shorter than     688 bp
  55% of sequence in fragments shorter than     762 bp
  60% of sequence in fragments shorter than     844 bp
  65% of sequence in fragments shorter than     933 bp
  70% of sequence in fragments shorter than    1037 bp
  75% of sequence in fragments shorter than    1146 bp
  80% of sequence in fragments shorter than    1287 bp
  85% of sequence in fragments shorter than    1470 bp
  90% of sequence in fragments shorter than    1728 bp
  95% of sequence in fragments shorter than    2179 bp

# BscAI.Tsp45I (GCATC+GTSAC) double digest, 7674 thousand fragments (3652926+4021761 sites), 388 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     322 bp
  25% of sequence in fragments shorter than     375 bp
  30% of sequence in fragments shorter than     433 bp
  35% of sequence in fragments shorter than     492 bp
  40% of sequence in fragments shorter than     552 bp
  45% of sequence in fragments shorter than     615 bp
  50% of sequence in fragments shorter than     681 bp
  55% of sequence in fragments shorter than     751 bp
  60% of sequence in fragments shorter than     828 bp
  65% of sequence in fragments shorter than     917 bp
  70% of sequence in fragments shorter than    1016 bp
  75% of sequence in fragments shorter than    1134 bp
  80% of sequence in fragments shorter than    1271 bp
  85% of sequence in fragments shorter than    1450 bp
  90% of sequence in fragments shorter than    1696 bp
  95% of sequence in fragments shorter than    2124 bp

# BspNCI.HpaI (CCAGA+GTTAAC) double digest, 7671 thousand fragments (7282436+389418 sites), 388 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     325 bp
  25% of sequence in fragments shorter than     380 bp
  30% of sequence in fragments shorter than     439 bp
  35% of sequence in fragments shorter than     500 bp
  40% of sequence in fragments shorter than     562 bp
  45% of sequence in fragments shorter than     622 bp
  50% of sequence in fragments shorter than     691 bp
  55% of sequence in fragments shorter than     764 bp
  60% of sequence in fragments shorter than     848 bp
  65% of sequence in fragments shorter than     936 bp
  70% of sequence in fragments shorter than    1040 bp
  75% of sequence in fragments shorter than    1150 bp
  80% of sequence in fragments shorter than    1292 bp
  85% of sequence in fragments shorter than    1477 bp
  90% of sequence in fragments shorter than    1737 bp
  95% of sequence in fragments shorter than    2195 bp

# BbvI.SspI (GCAGC+AATATT) double digest, 7666 thousand fragments (5290051+2377267 sites), 388 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     273 bp
  20% of sequence in fragments shorter than     334 bp
  25% of sequence in fragments shorter than     395 bp
  30% of sequence in fragments shorter than     456 bp
  35% of sequence in fragments shorter than     519 bp
  40% of sequence in fragments shorter than     585 bp
  45% of sequence in fragments shorter than     654 bp
  50% of sequence in fragments shorter than     725 bp
  55% of sequence in fragments shorter than     802 bp
  60% of sequence in fragments shorter than     882 bp
  65% of sequence in fragments shorter than     977 bp
  70% of sequence in fragments shorter than    1082 bp
  75% of sequence in fragments shorter than    1205 bp
  80% of sequence in fragments shorter than    1351 bp
  85% of sequence in fragments shorter than    1540 bp
  90% of sequence in fragments shorter than    1780 bp
  95% of sequence in fragments shorter than    2226 bp

# Hin4II.SacI (CCTTC+GAGCTC) double digest, 7662 thousand fragments (7059915+603323 sites), 388 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     276 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     399 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     527 bp
  40% of sequence in fragments shorter than     594 bp
  45% of sequence in fragments shorter than     667 bp
  50% of sequence in fragments shorter than     745 bp
  55% of sequence in fragments shorter than     830 bp
  60% of sequence in fragments shorter than     921 bp
  65% of sequence in fragments shorter than    1023 bp
  70% of sequence in fragments shorter than    1138 bp
  75% of sequence in fragments shorter than    1275 bp
  80% of sequence in fragments shorter than    1438 bp
  85% of sequence in fragments shorter than    1650 bp
  90% of sequence in fragments shorter than    1944 bp
  95% of sequence in fragments shorter than    2444 bp

# ApaI.BspKT6I (GGGCCC+GATC) double digest, 7659 thousand fragments (460339+7199434 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     187 bp
  15% of sequence in fragments shorter than     253 bp
  20% of sequence in fragments shorter than     311 bp
  25% of sequence in fragments shorter than     364 bp
  30% of sequence in fragments shorter than     420 bp
  35% of sequence in fragments shorter than     474 bp
  40% of sequence in fragments shorter than     532 bp
  45% of sequence in fragments shorter than     593 bp
  50% of sequence in fragments shorter than     658 bp
  55% of sequence in fragments shorter than     729 bp
  60% of sequence in fragments shorter than     806 bp
  65% of sequence in fragments shorter than     891 bp
  70% of sequence in fragments shorter than     988 bp
  75% of sequence in fragments shorter than    1092 bp
  80% of sequence in fragments shorter than    1227 bp
  85% of sequence in fragments shorter than    1399 bp
  90% of sequence in fragments shorter than    1649 bp
  95% of sequence in fragments shorter than    2085 bp

# BspNCI.SapI (CCAGA+GCTCTTC) double digest, 7659 thousand fragments (7282436+377161 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     327 bp
  25% of sequence in fragments shorter than     384 bp
  30% of sequence in fragments shorter than     444 bp
  35% of sequence in fragments shorter than     506 bp
  40% of sequence in fragments shorter than     567 bp
  45% of sequence in fragments shorter than     629 bp
  50% of sequence in fragments shorter than     700 bp
  55% of sequence in fragments shorter than     776 bp
  60% of sequence in fragments shorter than     862 bp
  65% of sequence in fragments shorter than     954 bp
  70% of sequence in fragments shorter than    1059 bp
  75% of sequence in fragments shorter than    1175 bp
  80% of sequence in fragments shorter than    1324 bp
  85% of sequence in fragments shorter than    1513 bp
  90% of sequence in fragments shorter than    1782 bp
  95% of sequence in fragments shorter than    2255 bp

# BspNCI.SmaI (CCAGA+CCCGGG) double digest, 7658 thousand fragments (7282436+376940 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     324 bp
  25% of sequence in fragments shorter than     380 bp
  30% of sequence in fragments shorter than     439 bp
  35% of sequence in fragments shorter than     499 bp
  40% of sequence in fragments shorter than     562 bp
  45% of sequence in fragments shorter than     622 bp
  50% of sequence in fragments shorter than     692 bp
  55% of sequence in fragments shorter than     766 bp
  60% of sequence in fragments shorter than     851 bp
  65% of sequence in fragments shorter than     941 bp
  70% of sequence in fragments shorter than    1046 bp
  75% of sequence in fragments shorter than    1159 bp
  80% of sequence in fragments shorter than    1306 bp
  85% of sequence in fragments shorter than    1496 bp
  90% of sequence in fragments shorter than    1764 bp
  95% of sequence in fragments shorter than    2240 bp

# AflIII.BccI (ACRYGT+CCATC) double digest, 7655 thousand fragments (1485394+6170927 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     266 bp
  20% of sequence in fragments shorter than     324 bp
  25% of sequence in fragments shorter than     381 bp
  30% of sequence in fragments shorter than     438 bp
  35% of sequence in fragments shorter than     496 bp
  40% of sequence in fragments shorter than     559 bp
  45% of sequence in fragments shorter than     624 bp
  50% of sequence in fragments shorter than     692 bp
  55% of sequence in fragments shorter than     767 bp
  60% of sequence in fragments shorter than     848 bp
  65% of sequence in fragments shorter than     939 bp
  70% of sequence in fragments shorter than    1042 bp
  75% of sequence in fragments shorter than    1156 bp
  80% of sequence in fragments shorter than    1300 bp
  85% of sequence in fragments shorter than    1489 bp
  90% of sequence in fragments shorter than    1756 bp
  95% of sequence in fragments shorter than    2248 bp

# HphI.PsiI (GGTGA+TTATAA) double digest, 7654 thousand fragments (6007336+1647912 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     369 bp
  30% of sequence in fragments shorter than     424 bp
  35% of sequence in fragments shorter than     480 bp
  40% of sequence in fragments shorter than     537 bp
  45% of sequence in fragments shorter than     594 bp
  50% of sequence in fragments shorter than     658 bp
  55% of sequence in fragments shorter than     723 bp
  60% of sequence in fragments shorter than     797 bp
  65% of sequence in fragments shorter than     872 bp
  70% of sequence in fragments shorter than     966 bp
  75% of sequence in fragments shorter than    1072 bp
  80% of sequence in fragments shorter than    1202 bp
  85% of sequence in fragments shorter than    1364 bp
  90% of sequence in fragments shorter than    1581 bp
  95% of sequence in fragments shorter than    1984 bp

# AvrII.Hin4II (CCTAGG+CCTTC) double digest, 7654 thousand fragments (594956+7059915 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     463 bp
  35% of sequence in fragments shorter than     527 bp
  40% of sequence in fragments shorter than     593 bp
  45% of sequence in fragments shorter than     665 bp
  50% of sequence in fragments shorter than     742 bp
  55% of sequence in fragments shorter than     825 bp
  60% of sequence in fragments shorter than     915 bp
  65% of sequence in fragments shorter than    1014 bp
  70% of sequence in fragments shorter than    1127 bp
  75% of sequence in fragments shorter than    1262 bp
  80% of sequence in fragments shorter than    1422 bp
  85% of sequence in fragments shorter than    1629 bp
  90% of sequence in fragments shorter than    1913 bp
  95% of sequence in fragments shorter than    2402 bp

# BthCI.FauI (GCNGC+CCCGC) double digest, 7649 thousand fragments (6209929+1439772 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     220 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     385 bp
  25% of sequence in fragments shorter than     471 bp
  30% of sequence in fragments shorter than     560 bp
  35% of sequence in fragments shorter than     652 bp
  40% of sequence in fragments shorter than     747 bp
  45% of sequence in fragments shorter than     848 bp
  50% of sequence in fragments shorter than     954 bp
  55% of sequence in fragments shorter than    1072 bp
  60% of sequence in fragments shorter than    1206 bp
  65% of sequence in fragments shorter than    1355 bp
  70% of sequence in fragments shorter than    1525 bp
  75% of sequence in fragments shorter than    1722 bp
  80% of sequence in fragments shorter than    1964 bp
  85% of sequence in fragments shorter than    2265 bp
  90% of sequence in fragments shorter than    2713 bp
  95% of sequence in fragments shorter than    3468 bp

# BamHI.BspNCI (GGATCC+CCAGA) double digest, 7647 thousand fragments (365999+7282436 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     202 bp
  15% of sequence in fragments shorter than     266 bp
  20% of sequence in fragments shorter than     326 bp
  25% of sequence in fragments shorter than     384 bp
  30% of sequence in fragments shorter than     444 bp
  35% of sequence in fragments shorter than     506 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     631 bp
  50% of sequence in fragments shorter than     702 bp
  55% of sequence in fragments shorter than     778 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     952 bp
  70% of sequence in fragments shorter than    1057 bp
  75% of sequence in fragments shorter than    1177 bp
  80% of sequence in fragments shorter than    1327 bp
  85% of sequence in fragments shorter than    1520 bp
  90% of sequence in fragments shorter than    1792 bp
  95% of sequence in fragments shorter than    2271 bp

# BspMI.BthCI (ACCTGC+GCNGC) double digest, 7647 thousand fragments (1438129+6209929 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     443 bp
  30% of sequence in fragments shorter than     525 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     697 bp
  45% of sequence in fragments shorter than     793 bp
  50% of sequence in fragments shorter than     891 bp
  55% of sequence in fragments shorter than     998 bp
  60% of sequence in fragments shorter than    1116 bp
  65% of sequence in fragments shorter than    1251 bp
  70% of sequence in fragments shorter than    1407 bp
  75% of sequence in fragments shorter than    1591 bp
  80% of sequence in fragments shorter than    1804 bp
  85% of sequence in fragments shorter than    2082 bp
  90% of sequence in fragments shorter than    2484 bp
  95% of sequence in fragments shorter than    3158 bp

# BspKT6I.EcoRV (GATC+GATATC) double digest, 7645 thousand fragments (7199434+446473 sites), 389 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     188 bp
  15% of sequence in fragments shorter than     254 bp
  20% of sequence in fragments shorter than     312 bp
  25% of sequence in fragments shorter than     365 bp
  30% of sequence in fragments shorter than     421 bp
  35% of sequence in fragments shorter than     475 bp
  40% of sequence in fragments shorter than     532 bp
  45% of sequence in fragments shorter than     594 bp
  50% of sequence in fragments shorter than     658 bp
  55% of sequence in fragments shorter than     728 bp
  60% of sequence in fragments shorter than     805 bp
  65% of sequence in fragments shorter than     888 bp
  70% of sequence in fragments shorter than     984 bp
  75% of sequence in fragments shorter than    1087 bp
  80% of sequence in fragments shorter than    1219 bp
  85% of sequence in fragments shorter than    1385 bp
  90% of sequence in fragments shorter than    1625 bp
  95% of sequence in fragments shorter than    2050 bp

# AvaI.BsmAI (CYCGRG+GTCTC) double digest, 7636 thousand fragments (1005714+6631585 sites), 390 bp average fragment length
   5% of sequence in fragments shorter than     121 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     267 bp
  20% of sequence in fragments shorter than     341 bp
  25% of sequence in fragments shorter than     412 bp
  30% of sequence in fragments shorter than     484 bp
  35% of sequence in fragments shorter than     556 bp
  40% of sequence in fragments shorter than     629 bp
  45% of sequence in fragments shorter than     711 bp
  50% of sequence in fragments shorter than     801 bp
  55% of sequence in fragments shorter than     897 bp
  60% of sequence in fragments shorter than    1005 bp
  65% of sequence in fragments shorter than    1125 bp
  70% of sequence in fragments shorter than    1265 bp
  75% of sequence in fragments shorter than    1432 bp
  80% of sequence in fragments shorter than    1636 bp
  85% of sequence in fragments shorter than    1904 bp
  90% of sequence in fragments shorter than    2275 bp
  95% of sequence in fragments shorter than    2962 bp

# HgaI.Hin4II (GACGC+CCTTC) double digest, 7631 thousand fragments (571889+7059915 sites), 390 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     402 bp
  30% of sequence in fragments shorter than     467 bp
  35% of sequence in fragments shorter than     530 bp
  40% of sequence in fragments shorter than     601 bp
  45% of sequence in fragments shorter than     675 bp
  50% of sequence in fragments shorter than     755 bp
  55% of sequence in fragments shorter than     840 bp
  60% of sequence in fragments shorter than     933 bp
  65% of sequence in fragments shorter than    1033 bp
  70% of sequence in fragments shorter than    1153 bp
  75% of sequence in fragments shorter than    1294 bp
  80% of sequence in fragments shorter than    1461 bp
  85% of sequence in fragments shorter than    1677 bp
  90% of sequence in fragments shorter than    1974 bp
  95% of sequence in fragments shorter than    2480 bp

# EciI.Hin4II (GGCGGA+CCTTC) double digest, 7630 thousand fragments (571273+7059915 sites), 390 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     276 bp
  20% of sequence in fragments shorter than     339 bp
  25% of sequence in fragments shorter than     396 bp
  30% of sequence in fragments shorter than     457 bp
  35% of sequence in fragments shorter than     521 bp
  40% of sequence in fragments shorter than     584 bp
  45% of sequence in fragments shorter than     654 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     900 bp
  65% of sequence in fragments shorter than    1000 bp
  70% of sequence in fragments shorter than    1108 bp
  75% of sequence in fragments shorter than    1240 bp
  80% of sequence in fragments shorter than    1397 bp
  85% of sequence in fragments shorter than    1602 bp
  90% of sequence in fragments shorter than    1882 bp
  95% of sequence in fragments shorter than    2367 bp

# Hin4II.MfeI (CCTTC+CAATTG) double digest, 7623 thousand fragments (7059915+564303 sites), 390 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     401 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     526 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     737 bp
  55% of sequence in fragments shorter than     819 bp
  60% of sequence in fragments shorter than     906 bp
  65% of sequence in fragments shorter than    1006 bp
  70% of sequence in fragments shorter than    1114 bp
  75% of sequence in fragments shorter than    1244 bp
  80% of sequence in fragments shorter than    1399 bp
  85% of sequence in fragments shorter than    1601 bp
  90% of sequence in fragments shorter than    1876 bp
  95% of sequence in fragments shorter than    2347 bp

# BspNCI.BstEII (CCAGA+GGTNACC) double digest, 7617 thousand fragments (7282436+335865 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     329 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     447 bp
  35% of sequence in fragments shorter than     509 bp
  40% of sequence in fragments shorter than     572 bp
  45% of sequence in fragments shorter than     635 bp
  50% of sequence in fragments shorter than     705 bp
  55% of sequence in fragments shorter than     782 bp
  60% of sequence in fragments shorter than     866 bp
  65% of sequence in fragments shorter than     961 bp
  70% of sequence in fragments shorter than    1067 bp
  75% of sequence in fragments shorter than    1182 bp
  80% of sequence in fragments shorter than    1332 bp
  85% of sequence in fragments shorter than    1526 bp
  90% of sequence in fragments shorter than    1799 bp
  95% of sequence in fragments shorter than    2279 bp

# ApoI.BscGI (RAATTY+CCCGT) double digest, 7612 thousand fragments (6382433+1231070 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     263 bp
  20% of sequence in fragments shorter than     321 bp
  25% of sequence in fragments shorter than     373 bp
  30% of sequence in fragments shorter than     432 bp
  35% of sequence in fragments shorter than     494 bp
  40% of sequence in fragments shorter than     557 bp
  45% of sequence in fragments shorter than     623 bp
  50% of sequence in fragments shorter than     693 bp
  55% of sequence in fragments shorter than     768 bp
  60% of sequence in fragments shorter than     851 bp
  65% of sequence in fragments shorter than     939 bp
  70% of sequence in fragments shorter than    1042 bp
  75% of sequence in fragments shorter than    1161 bp
  80% of sequence in fragments shorter than    1305 bp
  85% of sequence in fragments shorter than    1480 bp
  90% of sequence in fragments shorter than    1736 bp
  95% of sequence in fragments shorter than    2177 bp

# Hin4II.SphI (CCTTC+GCATGC) double digest, 7610 thousand fragments (7059915+550952 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     341 bp
  25% of sequence in fragments shorter than     399 bp
  30% of sequence in fragments shorter than     461 bp
  35% of sequence in fragments shorter than     526 bp
  40% of sequence in fragments shorter than     590 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     738 bp
  55% of sequence in fragments shorter than     821 bp
  60% of sequence in fragments shorter than     910 bp
  65% of sequence in fragments shorter than    1011 bp
  70% of sequence in fragments shorter than    1121 bp
  75% of sequence in fragments shorter than    1255 bp
  80% of sequence in fragments shorter than    1414 bp
  85% of sequence in fragments shorter than    1620 bp
  90% of sequence in fragments shorter than    1904 bp
  95% of sequence in fragments shorter than    2388 bp

# BccI.FauI (CCATC+CCCGC) double digest, 7610 thousand fragments (6170927+1439772 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     130 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     334 bp
  25% of sequence in fragments shorter than     397 bp
  30% of sequence in fragments shorter than     460 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     600 bp
  45% of sequence in fragments shorter than     673 bp
  50% of sequence in fragments shorter than     753 bp
  55% of sequence in fragments shorter than     841 bp
  60% of sequence in fragments shorter than     936 bp
  65% of sequence in fragments shorter than    1044 bp
  70% of sequence in fragments shorter than    1162 bp
  75% of sequence in fragments shorter than    1305 bp
  80% of sequence in fragments shorter than    1479 bp
  85% of sequence in fragments shorter than    1710 bp
  90% of sequence in fragments shorter than    2038 bp
  95% of sequence in fragments shorter than    2632 bp

# EsaBC3I.HphI (TCGA+GGTGA) double digest, 7610 thousand fragments (1603346+6007336 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     202 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     384 bp
  30% of sequence in fragments shorter than     443 bp
  35% of sequence in fragments shorter than     503 bp
  40% of sequence in fragments shorter than     565 bp
  45% of sequence in fragments shorter than     632 bp
  50% of sequence in fragments shorter than     700 bp
  55% of sequence in fragments shorter than     776 bp
  60% of sequence in fragments shorter than     859 bp
  65% of sequence in fragments shorter than     953 bp
  70% of sequence in fragments shorter than    1060 bp
  75% of sequence in fragments shorter than    1184 bp
  80% of sequence in fragments shorter than    1329 bp
  85% of sequence in fragments shorter than    1511 bp
  90% of sequence in fragments shorter than    1785 bp
  95% of sequence in fragments shorter than    2256 bp

# BsmAI.BspHI (GTCTC+TCATGA) double digest, 7609 thousand fragments (6631585+978290 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     393 bp
  30% of sequence in fragments shorter than     459 bp
  35% of sequence in fragments shorter than     524 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     663 bp
  50% of sequence in fragments shorter than     740 bp
  55% of sequence in fragments shorter than     825 bp
  60% of sequence in fragments shorter than     918 bp
  65% of sequence in fragments shorter than    1023 bp
  70% of sequence in fragments shorter than    1140 bp
  75% of sequence in fragments shorter than    1277 bp
  80% of sequence in fragments shorter than    1448 bp
  85% of sequence in fragments shorter than    1662 bp
  90% of sequence in fragments shorter than    1973 bp
  95% of sequence in fragments shorter than    2511 bp

# BccI.BspMI (CCATC+ACCTGC) double digest, 7608 thousand fragments (6170927+1438129 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     325 bp
  25% of sequence in fragments shorter than     381 bp
  30% of sequence in fragments shorter than     441 bp
  35% of sequence in fragments shorter than     499 bp
  40% of sequence in fragments shorter than     563 bp
  45% of sequence in fragments shorter than     629 bp
  50% of sequence in fragments shorter than     699 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     864 bp
  65% of sequence in fragments shorter than     960 bp
  70% of sequence in fragments shorter than    1069 bp
  75% of sequence in fragments shorter than    1198 bp
  80% of sequence in fragments shorter than    1353 bp
  85% of sequence in fragments shorter than    1550 bp
  90% of sequence in fragments shorter than    1838 bp
  95% of sequence in fragments shorter than    2344 bp

# BbvI.HpaII (GCAGC+CCGG) double digest, 7607 thousand fragments (5290051+2317722 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     126 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     283 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     441 bp
  30% of sequence in fragments shorter than     524 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     701 bp
  45% of sequence in fragments shorter than     800 bp
  50% of sequence in fragments shorter than     900 bp
  55% of sequence in fragments shorter than    1015 bp
  60% of sequence in fragments shorter than    1142 bp
  65% of sequence in fragments shorter than    1285 bp
  70% of sequence in fragments shorter than    1447 bp
  75% of sequence in fragments shorter than    1632 bp
  80% of sequence in fragments shorter than    1860 bp
  85% of sequence in fragments shorter than    2153 bp
  90% of sequence in fragments shorter than    2591 bp
  95% of sequence in fragments shorter than    3338 bp

# Hin4II.ScaI (CCTTC+AGTACT) double digest, 7603 thousand fragments (7059915+543793 sites), 391 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     463 bp
  35% of sequence in fragments shorter than     526 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     738 bp
  55% of sequence in fragments shorter than     820 bp
  60% of sequence in fragments shorter than     907 bp
  65% of sequence in fragments shorter than    1007 bp
  70% of sequence in fragments shorter than    1114 bp
  75% of sequence in fragments shorter than    1245 bp
  80% of sequence in fragments shorter than    1399 bp
  85% of sequence in fragments shorter than    1601 bp
  90% of sequence in fragments shorter than    1878 bp
  95% of sequence in fragments shorter than    2354 bp

# BspKT6I.SpeI (GATC+ACTAGT) double digest, 7599 thousand fragments (7199434+400286 sites), 392 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     189 bp
  15% of sequence in fragments shorter than     256 bp
  20% of sequence in fragments shorter than     314 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     423 bp
  35% of sequence in fragments shorter than     478 bp
  40% of sequence in fragments shorter than     537 bp
  45% of sequence in fragments shorter than     598 bp
  50% of sequence in fragments shorter than     663 bp
  55% of sequence in fragments shorter than     734 bp
  60% of sequence in fragments shorter than     811 bp
  65% of sequence in fragments shorter than     896 bp
  70% of sequence in fragments shorter than     993 bp
  75% of sequence in fragments shorter than    1096 bp
  80% of sequence in fragments shorter than    1230 bp
  85% of sequence in fragments shorter than    1398 bp
  90% of sequence in fragments shorter than    1643 bp
  95% of sequence in fragments shorter than    2063 bp

# BspKT6I.HpaI (GATC+GTTAAC) double digest, 7588 thousand fragments (7199434+389418 sites), 392 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     189 bp
  15% of sequence in fragments shorter than     256 bp
  20% of sequence in fragments shorter than     314 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     424 bp
  35% of sequence in fragments shorter than     478 bp
  40% of sequence in fragments shorter than     536 bp
  45% of sequence in fragments shorter than     598 bp
  50% of sequence in fragments shorter than     663 bp
  55% of sequence in fragments shorter than     733 bp
  60% of sequence in fragments shorter than     811 bp
  65% of sequence in fragments shorter than     895 bp
  70% of sequence in fragments shorter than     992 bp
  75% of sequence in fragments shorter than    1096 bp
  80% of sequence in fragments shorter than    1230 bp
  85% of sequence in fragments shorter than    1399 bp
  90% of sequence in fragments shorter than    1647 bp
  95% of sequence in fragments shorter than    2077 bp

# BscAI.MlyI (GCATC+GAGTC) double digest, 7584 thousand fragments (3652926+3931692 sites), 392 bp average fragment length
   5% of sequence in fragments shorter than     133 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     266 bp
  20% of sequence in fragments shorter than     323 bp
  25% of sequence in fragments shorter than     378 bp
  30% of sequence in fragments shorter than     435 bp
  35% of sequence in fragments shorter than     493 bp
  40% of sequence in fragments shorter than     553 bp
  45% of sequence in fragments shorter than     617 bp
  50% of sequence in fragments shorter than     683 bp
  55% of sequence in fragments shorter than     754 bp
  60% of sequence in fragments shorter than     831 bp
  65% of sequence in fragments shorter than     918 bp
  70% of sequence in fragments shorter than    1019 bp
  75% of sequence in fragments shorter than    1137 bp
  80% of sequence in fragments shorter than    1279 bp
  85% of sequence in fragments shorter than    1459 bp
  90% of sequence in fragments shorter than    1713 bp
  95% of sequence in fragments shorter than    2174 bp

# BspNCI.SexAI (CCAGA+ACCWGGT) double digest, 7579 thousand fragments (7282436+297900 sites), 393 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     329 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     447 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     570 bp
  45% of sequence in fragments shorter than     633 bp
  50% of sequence in fragments shorter than     703 bp
  55% of sequence in fragments shorter than     780 bp
  60% of sequence in fragments shorter than     864 bp
  65% of sequence in fragments shorter than     958 bp
  70% of sequence in fragments shorter than    1064 bp
  75% of sequence in fragments shorter than    1179 bp
  80% of sequence in fragments shorter than    1328 bp
  85% of sequence in fragments shorter than    1519 bp
  90% of sequence in fragments shorter than    1790 bp
  95% of sequence in fragments shorter than    2264 bp

# FokI.SspI (GGATG+AATATT) double digest, 7577 thousand fragments (5201116+2377267 sites), 393 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     271 bp
  20% of sequence in fragments shorter than     327 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     440 bp
  35% of sequence in fragments shorter than     498 bp
  40% of sequence in fragments shorter than     558 bp
  45% of sequence in fragments shorter than     621 bp
  50% of sequence in fragments shorter than     687 bp
  55% of sequence in fragments shorter than     756 bp
  60% of sequence in fragments shorter than     833 bp
  65% of sequence in fragments shorter than     917 bp
  70% of sequence in fragments shorter than    1008 bp
  75% of sequence in fragments shorter than    1114 bp
  80% of sequence in fragments shorter than    1245 bp
  85% of sequence in fragments shorter than    1407 bp
  90% of sequence in fragments shorter than    1640 bp
  95% of sequence in fragments shorter than    2047 bp

# BspKT6I.SapI (GATC+GCTCTTC) double digest, 7576 thousand fragments (7199434+377161 sites), 393 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     189 bp
  15% of sequence in fragments shorter than     257 bp
  20% of sequence in fragments shorter than     314 bp
  25% of sequence in fragments shorter than     368 bp
  30% of sequence in fragments shorter than     425 bp
  35% of sequence in fragments shorter than     480 bp
  40% of sequence in fragments shorter than     538 bp
  45% of sequence in fragments shorter than     600 bp
  50% of sequence in fragments shorter than     666 bp
  55% of sequence in fragments shorter than     737 bp
  60% of sequence in fragments shorter than     815 bp
  65% of sequence in fragments shorter than     900 bp
  70% of sequence in fragments shorter than     999 bp
  75% of sequence in fragments shorter than    1104 bp
  80% of sequence in fragments shorter than    1239 bp
  85% of sequence in fragments shorter than    1408 bp
  90% of sequence in fragments shorter than    1657 bp
  95% of sequence in fragments shorter than    2087 bp

# BspKT6I.SmaI (GATC+CCCGGG) double digest, 7575 thousand fragments (7199434+376940 sites), 393 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     188 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     318 bp
  25% of sequence in fragments shorter than     374 bp
  30% of sequence in fragments shorter than     431 bp
  35% of sequence in fragments shorter than     486 bp
  40% of sequence in fragments shorter than     548 bp
  45% of sequence in fragments shorter than     612 bp
  50% of sequence in fragments shorter than     679 bp
  55% of sequence in fragments shorter than     752 bp
  60% of sequence in fragments shorter than     832 bp
  65% of sequence in fragments shorter than     920 bp
  70% of sequence in fragments shorter than    1019 bp
  75% of sequence in fragments shorter than    1129 bp
  80% of sequence in fragments shorter than    1268 bp
  85% of sequence in fragments shorter than    1445 bp
  90% of sequence in fragments shorter than    1702 bp
  95% of sequence in fragments shorter than    2148 bp

# BspNCI.KpnI (CCAGA+GGTACC) double digest, 7569 thousand fragments (7282436+287974 sites), 393 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     332 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     450 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     569 bp
  45% of sequence in fragments shorter than     635 bp
  50% of sequence in fragments shorter than     702 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     863 bp
  65% of sequence in fragments shorter than     957 bp
  70% of sequence in fragments shorter than    1062 bp
  75% of sequence in fragments shorter than    1184 bp
  80% of sequence in fragments shorter than    1334 bp
  85% of sequence in fragments shorter than    1528 bp
  90% of sequence in fragments shorter than    1801 bp
  95% of sequence in fragments shorter than    2283 bp

# BspNCI.NheI (CCAGA+GCTAGC) double digest, 7569 thousand fragments (7282436+287384 sites), 393 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     329 bp
  25% of sequence in fragments shorter than     385 bp
  30% of sequence in fragments shorter than     445 bp
  35% of sequence in fragments shorter than     507 bp
  40% of sequence in fragments shorter than     572 bp
  45% of sequence in fragments shorter than     636 bp
  50% of sequence in fragments shorter than     706 bp
  55% of sequence in fragments shorter than     783 bp
  60% of sequence in fragments shorter than     863 bp
  65% of sequence in fragments shorter than     956 bp
  70% of sequence in fragments shorter than    1062 bp
  75% of sequence in fragments shorter than    1184 bp
  80% of sequence in fragments shorter than    1334 bp
  85% of sequence in fragments shorter than    1525 bp
  90% of sequence in fragments shorter than    1798 bp
  95% of sequence in fragments shorter than    2277 bp

# BamHI.BspKT6I (GGATCC+GATC) double digest, 7564 thousand fragments (365999+7199434 sites), 393 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     390 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     569 bp
  45% of sequence in fragments shorter than     634 bp
  50% of sequence in fragments shorter than     703 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     862 bp
  65% of sequence in fragments shorter than     952 bp
  70% of sequence in fragments shorter than    1050 bp
  75% of sequence in fragments shorter than    1167 bp
  80% of sequence in fragments shorter than    1309 bp
  85% of sequence in fragments shorter than    1491 bp
  90% of sequence in fragments shorter than    1749 bp
  95% of sequence in fragments shorter than    2213 bp

# AvaIII.BsmAI (ATGCAT+GTCTC) double digest, 7564 thousand fragments (933357+6631585 sites), 393 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     392 bp
  30% of sequence in fragments shorter than     456 bp
  35% of sequence in fragments shorter than     521 bp
  40% of sequence in fragments shorter than     586 bp
  45% of sequence in fragments shorter than     656 bp
  50% of sequence in fragments shorter than     732 bp
  55% of sequence in fragments shorter than     815 bp
  60% of sequence in fragments shorter than     905 bp
  65% of sequence in fragments shorter than    1006 bp
  70% of sequence in fragments shorter than    1121 bp
  75% of sequence in fragments shorter than    1256 bp
  80% of sequence in fragments shorter than    1424 bp
  85% of sequence in fragments shorter than    1635 bp
  90% of sequence in fragments shorter than    1944 bp
  95% of sequence in fragments shorter than    2471 bp

# HgiCI.HphI (GGYRCC+GGTGA) double digest, 7557 thousand fragments (1550877+6007336 sites), 394 bp average fragment length
   5% of sequence in fragments shorter than     129 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     266 bp
  20% of sequence in fragments shorter than     326 bp
  25% of sequence in fragments shorter than     385 bp
  30% of sequence in fragments shorter than     447 bp
  35% of sequence in fragments shorter than     511 bp
  40% of sequence in fragments shorter than     573 bp
  45% of sequence in fragments shorter than     641 bp
  50% of sequence in fragments shorter than     710 bp
  55% of sequence in fragments shorter than     790 bp
  60% of sequence in fragments shorter than     876 bp
  65% of sequence in fragments shorter than     977 bp
  70% of sequence in fragments shorter than    1089 bp
  75% of sequence in fragments shorter than    1223 bp
  80% of sequence in fragments shorter than    1377 bp
  85% of sequence in fragments shorter than    1575 bp
  90% of sequence in fragments shorter than    1869 bp
  95% of sequence in fragments shorter than    2377 bp

# BsmAI.StuI (GTCTC+AGGCCT) double digest, 7556 thousand fragments (6631585+925729 sites), 394 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     193 bp
  15% of sequence in fragments shorter than     260 bp
  20% of sequence in fragments shorter than     329 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     458 bp
  35% of sequence in fragments shorter than     524 bp
  40% of sequence in fragments shorter than     594 bp
  45% of sequence in fragments shorter than     671 bp
  50% of sequence in fragments shorter than     746 bp
  55% of sequence in fragments shorter than     835 bp
  60% of sequence in fragments shorter than     932 bp
  65% of sequence in fragments shorter than    1042 bp
  70% of sequence in fragments shorter than    1168 bp
  75% of sequence in fragments shorter than    1313 bp
  80% of sequence in fragments shorter than    1487 bp
  85% of sequence in fragments shorter than    1718 bp
  90% of sequence in fragments shorter than    2042 bp
  95% of sequence in fragments shorter than    2582 bp

# BsmI.HphI (GAATGC+GGTGA) double digest, 7556 thousand fragments (1549350+6007336 sites), 394 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     322 bp
  25% of sequence in fragments shorter than     380 bp
  30% of sequence in fragments shorter than     441 bp
  35% of sequence in fragments shorter than     503 bp
  40% of sequence in fragments shorter than     565 bp
  45% of sequence in fragments shorter than     631 bp
  50% of sequence in fragments shorter than     701 bp
  55% of sequence in fragments shorter than     774 bp
  60% of sequence in fragments shorter than     857 bp
  65% of sequence in fragments shorter than     949 bp
  70% of sequence in fragments shorter than    1049 bp
  75% of sequence in fragments shorter than    1169 bp
  80% of sequence in fragments shorter than    1314 bp
  85% of sequence in fragments shorter than    1492 bp
  90% of sequence in fragments shorter than    1749 bp
  95% of sequence in fragments shorter than    2179 bp

# ApaLI.Hin4II (GTGCAC+CCTTC) double digest, 7555 thousand fragments (496255+7059915 sites), 394 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     343 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     467 bp
  35% of sequence in fragments shorter than     529 bp
  40% of sequence in fragments shorter than     599 bp
  45% of sequence in fragments shorter than     672 bp
  50% of sequence in fragments shorter than     749 bp
  55% of sequence in fragments shorter than     833 bp
  60% of sequence in fragments shorter than     924 bp
  65% of sequence in fragments shorter than    1025 bp
  70% of sequence in fragments shorter than    1139 bp
  75% of sequence in fragments shorter than    1274 bp
  80% of sequence in fragments shorter than    1436 bp
  85% of sequence in fragments shorter than    1647 bp
  90% of sequence in fragments shorter than    1936 bp
  95% of sequence in fragments shorter than    2432 bp

# SfeI.Tsp45I (CTRYAG+GTSAC) double digest, 7552 thousand fragments (3531009+4021761 sites), 394 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     269 bp
  20% of sequence in fragments shorter than     327 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     438 bp
  35% of sequence in fragments shorter than     496 bp
  40% of sequence in fragments shorter than     556 bp
  45% of sequence in fragments shorter than     620 bp
  50% of sequence in fragments shorter than     685 bp
  55% of sequence in fragments shorter than     757 bp
  60% of sequence in fragments shorter than     838 bp
  65% of sequence in fragments shorter than     925 bp
  70% of sequence in fragments shorter than    1026 bp
  75% of sequence in fragments shorter than    1144 bp
  80% of sequence in fragments shorter than    1288 bp
  85% of sequence in fragments shorter than    1473 bp
  90% of sequence in fragments shorter than    1733 bp
  95% of sequence in fragments shorter than    2145 bp

# BspKT6I.BstEII (GATC+GGTNACC) double digest, 7534 thousand fragments (7199434+335865 sites), 395 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     189 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     371 bp
  30% of sequence in fragments shorter than     427 bp
  35% of sequence in fragments shorter than     482 bp
  40% of sequence in fragments shorter than     541 bp
  45% of sequence in fragments shorter than     604 bp
  50% of sequence in fragments shorter than     669 bp
  55% of sequence in fragments shorter than     741 bp
  60% of sequence in fragments shorter than     820 bp
  65% of sequence in fragments shorter than     906 bp
  70% of sequence in fragments shorter than    1005 bp
  75% of sequence in fragments shorter than    1110 bp
  80% of sequence in fragments shorter than    1247 bp
  85% of sequence in fragments shorter than    1419 bp
  90% of sequence in fragments shorter than    1670 bp
  95% of sequence in fragments shorter than    2112 bp

# BsmAI.NdeI (GTCTC+CATATG) double digest, 7534 thousand fragments (6631585+903360 sites), 395 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     330 bp
  25% of sequence in fragments shorter than     392 bp
  30% of sequence in fragments shorter than     455 bp
  35% of sequence in fragments shorter than     520 bp
  40% of sequence in fragments shorter than     585 bp
  45% of sequence in fragments shorter than     654 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     810 bp
  60% of sequence in fragments shorter than     900 bp
  65% of sequence in fragments shorter than     999 bp
  70% of sequence in fragments shorter than    1110 bp
  75% of sequence in fragments shorter than    1234 bp
  80% of sequence in fragments shorter than    1393 bp
  85% of sequence in fragments shorter than    1591 bp
  90% of sequence in fragments shorter than    1878 bp
  95% of sequence in fragments shorter than    2405 bp

# BthCI.PstI (GCNGC+CTGCAG) double digest, 7530 thousand fragments (6209929+1321469 sites), 395 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     239 bp
  15% of sequence in fragments shorter than     325 bp
  20% of sequence in fragments shorter than     413 bp
  25% of sequence in fragments shorter than     503 bp
  30% of sequence in fragments shorter than     594 bp
  35% of sequence in fragments shorter than     689 bp
  40% of sequence in fragments shorter than     790 bp
  45% of sequence in fragments shorter than     891 bp
  50% of sequence in fragments shorter than    1005 bp
  55% of sequence in fragments shorter than    1124 bp
  60% of sequence in fragments shorter than    1260 bp
  65% of sequence in fragments shorter than    1410 bp
  70% of sequence in fragments shorter than    1585 bp
  75% of sequence in fragments shorter than    1777 bp
  80% of sequence in fragments shorter than    2020 bp
  85% of sequence in fragments shorter than    2319 bp
  90% of sequence in fragments shorter than    2762 bp
  95% of sequence in fragments shorter than    3513 bp

# ApaI.Hin4II (GGGCCC+CCTTC) double digest, 7519 thousand fragments (460339+7059915 sites), 396 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     282 bp
  20% of sequence in fragments shorter than     345 bp
  25% of sequence in fragments shorter than     410 bp
  30% of sequence in fragments shorter than     476 bp
  35% of sequence in fragments shorter than     541 bp
  40% of sequence in fragments shorter than     615 bp
  45% of sequence in fragments shorter than     691 bp
  50% of sequence in fragments shorter than     774 bp
  55% of sequence in fragments shorter than     862 bp
  60% of sequence in fragments shorter than     959 bp
  65% of sequence in fragments shorter than    1063 bp
  70% of sequence in fragments shorter than    1188 bp
  75% of sequence in fragments shorter than    1333 bp
  80% of sequence in fragments shorter than    1507 bp
  85% of sequence in fragments shorter than    1729 bp
  90% of sequence in fragments shorter than    2039 bp
  95% of sequence in fragments shorter than    2569 bp

# FokI.HpaII (GGATG+CCGG) double digest, 7518 thousand fragments (5201116+2317722 sites), 396 bp average fragment length
   5% of sequence in fragments shorter than     128 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     276 bp
  20% of sequence in fragments shorter than     345 bp
  25% of sequence in fragments shorter than     411 bp
  30% of sequence in fragments shorter than     481 bp
  35% of sequence in fragments shorter than     553 bp
  40% of sequence in fragments shorter than     628 bp
  45% of sequence in fragments shorter than     707 bp
  50% of sequence in fragments shorter than     792 bp
  55% of sequence in fragments shorter than     884 bp
  60% of sequence in fragments shorter than     980 bp
  65% of sequence in fragments shorter than    1089 bp
  70% of sequence in fragments shorter than    1213 bp
  75% of sequence in fragments shorter than    1361 bp
  80% of sequence in fragments shorter than    1533 bp
  85% of sequence in fragments shorter than    1764 bp
  90% of sequence in fragments shorter than    2094 bp
  95% of sequence in fragments shorter than    2696 bp

# BspNCI.BsrBI (CCAGA+CCGCTC) double digest, 7517 thousand fragments (7282436+235523 sites), 396 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     332 bp
  25% of sequence in fragments shorter than     390 bp
  30% of sequence in fragments shorter than     452 bp
  35% of sequence in fragments shorter than     515 bp
  40% of sequence in fragments shorter than     578 bp
  45% of sequence in fragments shorter than     643 bp
  50% of sequence in fragments shorter than     714 bp
  55% of sequence in fragments shorter than     792 bp
  60% of sequence in fragments shorter than     877 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1080 bp
  75% of sequence in fragments shorter than    1199 bp
  80% of sequence in fragments shorter than    1352 bp
  85% of sequence in fragments shorter than    1547 bp
  90% of sequence in fragments shorter than    1823 bp
  95% of sequence in fragments shorter than    2303 bp

# BspNCI.KasI (CCAGA+GGCGCC) double digest, 7512 thousand fragments (7282436+230381 sites), 396 bp average fragment length
   5% of sequence in fragments shorter than     133 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     511 bp
  40% of sequence in fragments shorter than     573 bp
  45% of sequence in fragments shorter than     636 bp
  50% of sequence in fragments shorter than     707 bp
  55% of sequence in fragments shorter than     783 bp
  60% of sequence in fragments shorter than     867 bp
  65% of sequence in fragments shorter than     962 bp
  70% of sequence in fragments shorter than    1068 bp
  75% of sequence in fragments shorter than    1183 bp
  80% of sequence in fragments shorter than    1334 bp
  85% of sequence in fragments shorter than    1527 bp
  90% of sequence in fragments shorter than    1799 bp
  95% of sequence in fragments shorter than    2280 bp

# BspNCI.SwaI (CCAGA+ATTTAAAT) double digest, 7508 thousand fragments (7282436+226602 sites), 396 bp average fragment length
   5% of sequence in fragments shorter than     133 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     271 bp
  20% of sequence in fragments shorter than     332 bp
  25% of sequence in fragments shorter than     390 bp
  30% of sequence in fragments shorter than     450 bp
  35% of sequence in fragments shorter than     511 bp
  40% of sequence in fragments shorter than     573 bp
  45% of sequence in fragments shorter than     635 bp
  50% of sequence in fragments shorter than     705 bp
  55% of sequence in fragments shorter than     780 bp
  60% of sequence in fragments shorter than     863 bp
  65% of sequence in fragments shorter than     955 bp
  70% of sequence in fragments shorter than    1060 bp
  75% of sequence in fragments shorter than    1172 bp
  80% of sequence in fragments shorter than    1319 bp
  85% of sequence in fragments shorter than    1507 bp
  90% of sequence in fragments shorter than    1770 bp
  95% of sequence in fragments shorter than    2237 bp

# EcoRV.Hin4II (GATATC+CCTTC) double digest, 7505 thousand fragments (446473+7059915 sites), 397 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     282 bp
  20% of sequence in fragments shorter than     344 bp
  25% of sequence in fragments shorter than     405 bp
  30% of sequence in fragments shorter than     471 bp
  35% of sequence in fragments shorter than     532 bp
  40% of sequence in fragments shorter than     601 bp
  45% of sequence in fragments shorter than     674 bp
  50% of sequence in fragments shorter than     752 bp
  55% of sequence in fragments shorter than     836 bp
  60% of sequence in fragments shorter than     925 bp
  65% of sequence in fragments shorter than    1025 bp
  70% of sequence in fragments shorter than    1137 bp
  75% of sequence in fragments shorter than    1272 bp
  80% of sequence in fragments shorter than    1431 bp
  85% of sequence in fragments shorter than    1637 bp
  90% of sequence in fragments shorter than    1920 bp
  95% of sequence in fragments shorter than    2407 bp

# BsrI.HhaI (ACTGG+GCGC) double digest, 7497 thousand fragments (5741310+1756502 sites), 397 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     273 bp
  20% of sequence in fragments shorter than     334 bp
  25% of sequence in fragments shorter than     394 bp
  30% of sequence in fragments shorter than     457 bp
  35% of sequence in fragments shorter than     520 bp
  40% of sequence in fragments shorter than     587 bp
  45% of sequence in fragments shorter than     657 bp
  50% of sequence in fragments shorter than     719 bp
  55% of sequence in fragments shorter than     798 bp
  60% of sequence in fragments shorter than     880 bp
  65% of sequence in fragments shorter than     977 bp
  70% of sequence in fragments shorter than    1086 bp
  75% of sequence in fragments shorter than    1213 bp
  80% of sequence in fragments shorter than    1368 bp
  85% of sequence in fragments shorter than    1562 bp
  90% of sequence in fragments shorter than    1833 bp
  95% of sequence in fragments shorter than    2304 bp

# BspKT6I.SexAI (GATC+ACCWGGT) double digest, 7496 thousand fragments (7199434+297900 sites), 397 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     190 bp
  15% of sequence in fragments shorter than     260 bp
  20% of sequence in fragments shorter than     318 bp
  25% of sequence in fragments shorter than     374 bp
  30% of sequence in fragments shorter than     430 bp
  35% of sequence in fragments shorter than     485 bp
  40% of sequence in fragments shorter than     546 bp
  45% of sequence in fragments shorter than     608 bp
  50% of sequence in fragments shorter than     675 bp
  55% of sequence in fragments shorter than     747 bp
  60% of sequence in fragments shorter than     826 bp
  65% of sequence in fragments shorter than     912 bp
  70% of sequence in fragments shorter than    1012 bp
  75% of sequence in fragments shorter than    1118 bp
  80% of sequence in fragments shorter than    1256 bp
  85% of sequence in fragments shorter than    1428 bp
  90% of sequence in fragments shorter than    1679 bp
  95% of sequence in fragments shorter than    2119 bp

# AflIII.HphI (ACRYGT+GGTGA) double digest, 7492 thousand fragments (1485394+6007336 sites), 397 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     269 bp
  20% of sequence in fragments shorter than     327 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     438 bp
  35% of sequence in fragments shorter than     496 bp
  40% of sequence in fragments shorter than     556 bp
  45% of sequence in fragments shorter than     619 bp
  50% of sequence in fragments shorter than     685 bp
  55% of sequence in fragments shorter than     756 bp
  60% of sequence in fragments shorter than     833 bp
  65% of sequence in fragments shorter than     920 bp
  70% of sequence in fragments shorter than    1021 bp
  75% of sequence in fragments shorter than    1138 bp
  80% of sequence in fragments shorter than    1277 bp
  85% of sequence in fragments shorter than    1440 bp
  90% of sequence in fragments shorter than    1679 bp
  95% of sequence in fragments shorter than    2108 bp

# BccI.PstI (CCATC+CTGCAG) double digest, 7491 thousand fragments (6170927+1321469 sites), 397 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     269 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     388 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     511 bp
  40% of sequence in fragments shorter than     577 bp
  45% of sequence in fragments shorter than     645 bp
  50% of sequence in fragments shorter than     718 bp
  55% of sequence in fragments shorter than     799 bp
  60% of sequence in fragments shorter than     887 bp
  65% of sequence in fragments shorter than     987 bp
  70% of sequence in fragments shorter than    1095 bp
  75% of sequence in fragments shorter than    1226 bp
  80% of sequence in fragments shorter than    1384 bp
  85% of sequence in fragments shorter than    1595 bp
  90% of sequence in fragments shorter than    1895 bp
  95% of sequence in fragments shorter than    2443 bp

# CviQI.SspI (GTAC+AATATT) double digest, 7491 thousand fragments (5115078+2377267 sites), 397 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     391 bp
  30% of sequence in fragments shorter than     452 bp
  35% of sequence in fragments shorter than     513 bp
  40% of sequence in fragments shorter than     577 bp
  45% of sequence in fragments shorter than     646 bp
  50% of sequence in fragments shorter than     716 bp
  55% of sequence in fragments shorter than     792 bp
  60% of sequence in fragments shorter than     878 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1080 bp
  75% of sequence in fragments shorter than    1211 bp
  80% of sequence in fragments shorter than    1359 bp
  85% of sequence in fragments shorter than    1552 bp
  90% of sequence in fragments shorter than    1845 bp
  95% of sequence in fragments shorter than    2379 bp

# BsmAI.HindIII (GTCTC+AAGCTT) double digest, 7491 thousand fragments (6631585+860365 sites), 397 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     395 bp
  30% of sequence in fragments shorter than     461 bp
  35% of sequence in fragments shorter than     524 bp
  40% of sequence in fragments shorter than     592 bp
  45% of sequence in fragments shorter than     664 bp
  50% of sequence in fragments shorter than     743 bp
  55% of sequence in fragments shorter than     828 bp
  60% of sequence in fragments shorter than     921 bp
  65% of sequence in fragments shorter than    1026 bp
  70% of sequence in fragments shorter than    1143 bp
  75% of sequence in fragments shorter than    1283 bp
  80% of sequence in fragments shorter than    1455 bp
  85% of sequence in fragments shorter than    1683 bp
  90% of sequence in fragments shorter than    2008 bp
  95% of sequence in fragments shorter than    2581 bp

# BspKT6I.KpnI (GATC+GGTACC) double digest, 7486 thousand fragments (7199434+287974 sites), 398 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     191 bp
  15% of sequence in fragments shorter than     258 bp
  20% of sequence in fragments shorter than     317 bp
  25% of sequence in fragments shorter than     372 bp
  30% of sequence in fragments shorter than     429 bp
  35% of sequence in fragments shorter than     485 bp
  40% of sequence in fragments shorter than     546 bp
  45% of sequence in fragments shorter than     609 bp
  50% of sequence in fragments shorter than     676 bp
  55% of sequence in fragments shorter than     748 bp
  60% of sequence in fragments shorter than     828 bp
  65% of sequence in fragments shorter than     915 bp
  70% of sequence in fragments shorter than    1009 bp
  75% of sequence in fragments shorter than    1120 bp
  80% of sequence in fragments shorter than    1257 bp
  85% of sequence in fragments shorter than    1432 bp
  90% of sequence in fragments shorter than    1689 bp
  95% of sequence in fragments shorter than    2134 bp

# BspKT6I.NheI (GATC+GCTAGC) double digest, 7486 thousand fragments (7199434+287384 sites), 398 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     191 bp
  15% of sequence in fragments shorter than     261 bp
  20% of sequence in fragments shorter than     321 bp
  25% of sequence in fragments shorter than     376 bp
  30% of sequence in fragments shorter than     433 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     549 bp
  45% of sequence in fragments shorter than     612 bp
  50% of sequence in fragments shorter than     678 bp
  55% of sequence in fragments shorter than     751 bp
  60% of sequence in fragments shorter than     830 bp
  65% of sequence in fragments shorter than     917 bp
  70% of sequence in fragments shorter than    1017 bp
  75% of sequence in fragments shorter than    1123 bp
  80% of sequence in fragments shorter than    1261 bp
  85% of sequence in fragments shorter than    1434 bp
  90% of sequence in fragments shorter than    1685 bp
  95% of sequence in fragments shorter than    2117 bp

# AccI.ApoI (GTMKAC+RAATTY) double digest, 7482 thousand fragments (1101063+6382433 sites), 398 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     203 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     326 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     450 bp
  35% of sequence in fragments shorter than     516 bp
  40% of sequence in fragments shorter than     585 bp
  45% of sequence in fragments shorter than     657 bp
  50% of sequence in fragments shorter than     734 bp
  55% of sequence in fragments shorter than     814 bp
  60% of sequence in fragments shorter than     904 bp
  65% of sequence in fragments shorter than    1011 bp
  70% of sequence in fragments shorter than    1133 bp
  75% of sequence in fragments shorter than    1282 bp
  80% of sequence in fragments shorter than    1449 bp
  85% of sequence in fragments shorter than    1697 bp
  90% of sequence in fragments shorter than    2069 bp
  95% of sequence in fragments shorter than    2779 bp

# BsmAI.XbaI (GTCTC+TCTAGA) double digest, 7482 thousand fragments (6631585+850998 sites), 398 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     266 bp
  20% of sequence in fragments shorter than     333 bp
  25% of sequence in fragments shorter than     397 bp
  30% of sequence in fragments shorter than     465 bp
  35% of sequence in fragments shorter than     532 bp
  40% of sequence in fragments shorter than     600 bp
  45% of sequence in fragments shorter than     672 bp
  50% of sequence in fragments shorter than     749 bp
  55% of sequence in fragments shorter than     833 bp
  60% of sequence in fragments shorter than     921 bp
  65% of sequence in fragments shorter than    1024 bp
  70% of sequence in fragments shorter than    1136 bp
  75% of sequence in fragments shorter than    1273 bp
  80% of sequence in fragments shorter than    1422 bp
  85% of sequence in fragments shorter than    1626 bp
  90% of sequence in fragments shorter than    1910 bp
  95% of sequence in fragments shorter than    2423 bp

# BsmAI.EcoRI (GTCTC+GAATTC) double digest, 7478 thousand fragments (6631585+847401 sites), 398 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     333 bp
  25% of sequence in fragments shorter than     394 bp
  30% of sequence in fragments shorter than     461 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     596 bp
  45% of sequence in fragments shorter than     669 bp
  50% of sequence in fragments shorter than     745 bp
  55% of sequence in fragments shorter than     830 bp
  60% of sequence in fragments shorter than     924 bp
  65% of sequence in fragments shorter than    1030 bp
  70% of sequence in fragments shorter than    1150 bp
  75% of sequence in fragments shorter than    1292 bp
  80% of sequence in fragments shorter than    1462 bp
  85% of sequence in fragments shorter than    1679 bp
  90% of sequence in fragments shorter than    1994 bp
  95% of sequence in fragments shorter than    2525 bp

# ApoI.PpuMI (RAATTY+RGGWCCY) double digest, 7467 thousand fragments (6382433+1085138 sites), 399 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     267 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     448 bp
  35% of sequence in fragments shorter than     512 bp
  40% of sequence in fragments shorter than     575 bp
  45% of sequence in fragments shorter than     643 bp
  50% of sequence in fragments shorter than     714 bp
  55% of sequence in fragments shorter than     791 bp
  60% of sequence in fragments shorter than     874 bp
  65% of sequence in fragments shorter than     965 bp
  70% of sequence in fragments shorter than    1071 bp
  75% of sequence in fragments shorter than    1196 bp
  80% of sequence in fragments shorter than    1347 bp
  85% of sequence in fragments shorter than    1539 bp
  90% of sequence in fragments shorter than    1820 bp
  95% of sequence in fragments shorter than    2307 bp

# ApoI.PvuII (RAATTY+CAGCTG) double digest, 7466 thousand fragments (6382433+1084260 sites), 399 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     267 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     448 bp
  35% of sequence in fragments shorter than     512 bp
  40% of sequence in fragments shorter than     578 bp
  45% of sequence in fragments shorter than     646 bp
  50% of sequence in fragments shorter than     717 bp
  55% of sequence in fragments shorter than     798 bp
  60% of sequence in fragments shorter than     882 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1083 bp
  75% of sequence in fragments shorter than    1210 bp
  80% of sequence in fragments shorter than    1356 bp
  85% of sequence in fragments shorter than    1550 bp
  90% of sequence in fragments shorter than    1835 bp
  95% of sequence in fragments shorter than    2344 bp

# MlyI.SfeI (GAGTC+CTRYAG) double digest, 7462 thousand fragments (3931692+3531009 sites), 399 bp average fragment length
   5% of sequence in fragments shorter than     129 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     271 bp
  20% of sequence in fragments shorter than     329 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     446 bp
  35% of sequence in fragments shorter than     504 bp
  40% of sequence in fragments shorter than     566 bp
  45% of sequence in fragments shorter than     632 bp
  50% of sequence in fragments shorter than     697 bp
  55% of sequence in fragments shorter than     773 bp
  60% of sequence in fragments shorter than     857 bp
  65% of sequence in fragments shorter than     949 bp
  70% of sequence in fragments shorter than    1051 bp
  75% of sequence in fragments shorter than    1174 bp
  80% of sequence in fragments shorter than    1318 bp
  85% of sequence in fragments shorter than    1497 bp
  90% of sequence in fragments shorter than    1763 bp
  95% of sequence in fragments shorter than    2202 bp

# Hin4II.SpeI (CCTTC+ACTAGT) double digest, 7459 thousand fragments (7059915+400286 sites), 399 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     218 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     409 bp
  30% of sequence in fragments shorter than     471 bp
  35% of sequence in fragments shorter than     533 bp
  40% of sequence in fragments shorter than     602 bp
  45% of sequence in fragments shorter than     677 bp
  50% of sequence in fragments shorter than     753 bp
  55% of sequence in fragments shorter than     838 bp
  60% of sequence in fragments shorter than     928 bp
  65% of sequence in fragments shorter than    1031 bp
  70% of sequence in fragments shorter than    1146 bp
  75% of sequence in fragments shorter than    1281 bp
  80% of sequence in fragments shorter than    1443 bp
  85% of sequence in fragments shorter than    1652 bp
  90% of sequence in fragments shorter than    1939 bp
  95% of sequence in fragments shorter than    2430 bp

# AclI.BspNCI (AACGTT+CCAGA) double digest, 7454 thousand fragments (172706+7282436 sites), 399 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     272 bp
  20% of sequence in fragments shorter than     335 bp
  25% of sequence in fragments shorter than     393 bp
  30% of sequence in fragments shorter than     454 bp
  35% of sequence in fragments shorter than     516 bp
  40% of sequence in fragments shorter than     579 bp
  45% of sequence in fragments shorter than     643 bp
  50% of sequence in fragments shorter than     714 bp
  55% of sequence in fragments shorter than     791 bp
  60% of sequence in fragments shorter than     874 bp
  65% of sequence in fragments shorter than     968 bp
  70% of sequence in fragments shorter than    1071 bp
  75% of sequence in fragments shorter than    1188 bp
  80% of sequence in fragments shorter than    1338 bp
  85% of sequence in fragments shorter than    1528 bp
  90% of sequence in fragments shorter than    1795 bp
  95% of sequence in fragments shorter than    2251 bp

# Hin4II.HpaI (CCTTC+GTTAAC) double digest, 7448 thousand fragments (7059915+389418 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     217 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     410 bp
  30% of sequence in fragments shorter than     474 bp
  35% of sequence in fragments shorter than     537 bp
  40% of sequence in fragments shorter than     608 bp
  45% of sequence in fragments shorter than     681 bp
  50% of sequence in fragments shorter than     759 bp
  55% of sequence in fragments shorter than     844 bp
  60% of sequence in fragments shorter than     935 bp
  65% of sequence in fragments shorter than    1034 bp
  70% of sequence in fragments shorter than    1151 bp
  75% of sequence in fragments shorter than    1287 bp
  80% of sequence in fragments shorter than    1448 bp
  85% of sequence in fragments shorter than    1658 bp
  90% of sequence in fragments shorter than    1947 bp
  95% of sequence in fragments shorter than    2438 bp

# BfiI.BsrI (ACTGGG+ACTGG) double digest, 7447 thousand fragments (1706594+5741310 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     350 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     585 bp
  35% of sequence in fragments shorter than     663 bp
  40% of sequence in fragments shorter than     736 bp
  45% of sequence in fragments shorter than     822 bp
  50% of sequence in fragments shorter than     909 bp
  55% of sequence in fragments shorter than    1008 bp
  60% of sequence in fragments shorter than    1112 bp
  65% of sequence in fragments shorter than    1231 bp
  70% of sequence in fragments shorter than    1367 bp
  75% of sequence in fragments shorter than    1519 bp
  80% of sequence in fragments shorter than    1708 bp
  85% of sequence in fragments shorter than    1948 bp
  90% of sequence in fragments shorter than    2287 bp
  95% of sequence in fragments shorter than    2902 bp

# FauI.HphI (CCCGC+GGTGA) double digest, 7446 thousand fragments (1439772+6007336 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     275 bp
  20% of sequence in fragments shorter than     343 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     474 bp
  35% of sequence in fragments shorter than     542 bp
  40% of sequence in fragments shorter than     610 bp
  45% of sequence in fragments shorter than     686 bp
  50% of sequence in fragments shorter than     766 bp
  55% of sequence in fragments shorter than     851 bp
  60% of sequence in fragments shorter than     946 bp
  65% of sequence in fragments shorter than    1054 bp
  70% of sequence in fragments shorter than    1180 bp
  75% of sequence in fragments shorter than    1320 bp
  80% of sequence in fragments shorter than    1484 bp
  85% of sequence in fragments shorter than    1710 bp
  90% of sequence in fragments shorter than    2021 bp
  95% of sequence in fragments shorter than    2575 bp

# BspMI.HphI (ACCTGC+GGTGA) double digest, 7444 thousand fragments (1438129+6007336 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     275 bp
  20% of sequence in fragments shorter than     336 bp
  25% of sequence in fragments shorter than     394 bp
  30% of sequence in fragments shorter than     454 bp
  35% of sequence in fragments shorter than     516 bp
  40% of sequence in fragments shorter than     579 bp
  45% of sequence in fragments shorter than     646 bp
  50% of sequence in fragments shorter than     718 bp
  55% of sequence in fragments shorter than     796 bp
  60% of sequence in fragments shorter than     880 bp
  65% of sequence in fragments shorter than     975 bp
  70% of sequence in fragments shorter than    1086 bp
  75% of sequence in fragments shorter than    1216 bp
  80% of sequence in fragments shorter than    1366 bp
  85% of sequence in fragments shorter than    1563 bp
  90% of sequence in fragments shorter than    1852 bp
  95% of sequence in fragments shorter than    2327 bp

# BspNCI.PacI (CCAGA+TTAATTAA) double digest, 7441 thousand fragments (7282436+159455 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     273 bp
  20% of sequence in fragments shorter than     336 bp
  25% of sequence in fragments shorter than     394 bp
  30% of sequence in fragments shorter than     455 bp
  35% of sequence in fragments shorter than     517 bp
  40% of sequence in fragments shorter than     580 bp
  45% of sequence in fragments shorter than     644 bp
  50% of sequence in fragments shorter than     714 bp
  55% of sequence in fragments shorter than     791 bp
  60% of sequence in fragments shorter than     874 bp
  65% of sequence in fragments shorter than     970 bp
  70% of sequence in fragments shorter than    1075 bp
  75% of sequence in fragments shorter than    1191 bp
  80% of sequence in fragments shorter than    1341 bp
  85% of sequence in fragments shorter than    1532 bp
  90% of sequence in fragments shorter than    1802 bp
  95% of sequence in fragments shorter than    2280 bp

# BscGI.BthCI (CCCGT+GCNGC) double digest, 7440 thousand fragments (1231070+6209929 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     132 bp
  10% of sequence in fragments shorter than     218 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     447 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     715 bp
  45% of sequence in fragments shorter than     816 bp
  50% of sequence in fragments shorter than     923 bp
  55% of sequence in fragments shorter than    1040 bp
  60% of sequence in fragments shorter than    1168 bp
  65% of sequence in fragments shorter than    1315 bp
  70% of sequence in fragments shorter than    1481 bp
  75% of sequence in fragments shorter than    1668 bp
  80% of sequence in fragments shorter than    1902 bp
  85% of sequence in fragments shorter than    2194 bp
  90% of sequence in fragments shorter than    2619 bp
  95% of sequence in fragments shorter than    3341 bp

# BspNCI.Hpy99I (CCAGA+CGWCG) double digest, 7439 thousand fragments (7282436+157917 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     273 bp
  20% of sequence in fragments shorter than     335 bp
  25% of sequence in fragments shorter than     394 bp
  30% of sequence in fragments shorter than     456 bp
  35% of sequence in fragments shorter than     519 bp
  40% of sequence in fragments shorter than     582 bp
  45% of sequence in fragments shorter than     648 bp
  50% of sequence in fragments shorter than     720 bp
  55% of sequence in fragments shorter than     798 bp
  60% of sequence in fragments shorter than     883 bp
  65% of sequence in fragments shorter than     982 bp
  70% of sequence in fragments shorter than    1089 bp
  75% of sequence in fragments shorter than    1208 bp
  80% of sequence in fragments shorter than    1362 bp
  85% of sequence in fragments shorter than    1559 bp
  90% of sequence in fragments shorter than    1838 bp
  95% of sequence in fragments shorter than    2326 bp

# Hin4II.SapI (CCTTC+GCTCTTC) double digest, 7436 thousand fragments (7059915+377161 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     217 bp
  15% of sequence in fragments shorter than     286 bp
  20% of sequence in fragments shorter than     348 bp
  25% of sequence in fragments shorter than     413 bp
  30% of sequence in fragments shorter than     479 bp
  35% of sequence in fragments shorter than     543 bp
  40% of sequence in fragments shorter than     616 bp
  45% of sequence in fragments shorter than     691 bp
  50% of sequence in fragments shorter than     772 bp
  55% of sequence in fragments shorter than     859 bp
  60% of sequence in fragments shorter than     955 bp
  65% of sequence in fragments shorter than    1056 bp
  70% of sequence in fragments shorter than    1178 bp
  75% of sequence in fragments shorter than    1320 bp
  80% of sequence in fragments shorter than    1489 bp
  85% of sequence in fragments shorter than    1709 bp
  90% of sequence in fragments shorter than    2009 bp
  95% of sequence in fragments shorter than    2527 bp

# Hin4II.SmaI (CCTTC+CCCGGG) double digest, 7436 thousand fragments (7059915+376940 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     218 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     409 bp
  30% of sequence in fragments shorter than     473 bp
  35% of sequence in fragments shorter than     536 bp
  40% of sequence in fragments shorter than     607 bp
  45% of sequence in fragments shorter than     681 bp
  50% of sequence in fragments shorter than     759 bp
  55% of sequence in fragments shorter than     845 bp
  60% of sequence in fragments shorter than     937 bp
  65% of sequence in fragments shorter than    1036 bp
  70% of sequence in fragments shorter than    1155 bp
  75% of sequence in fragments shorter than    1293 bp
  80% of sequence in fragments shorter than    1458 bp
  85% of sequence in fragments shorter than    1672 bp
  90% of sequence in fragments shorter than    1964 bp
  95% of sequence in fragments shorter than    2467 bp

# BspKT6I.BsrBI (GATC+CCGCTC) double digest, 7434 thousand fragments (7199434+235523 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     321 bp
  25% of sequence in fragments shorter than     377 bp
  30% of sequence in fragments shorter than     434 bp
  35% of sequence in fragments shorter than     489 bp
  40% of sequence in fragments shorter than     551 bp
  45% of sequence in fragments shorter than     614 bp
  50% of sequence in fragments shorter than     681 bp
  55% of sequence in fragments shorter than     753 bp
  60% of sequence in fragments shorter than     833 bp
  65% of sequence in fragments shorter than     920 bp
  70% of sequence in fragments shorter than    1019 bp
  75% of sequence in fragments shorter than    1128 bp
  80% of sequence in fragments shorter than    1266 bp
  85% of sequence in fragments shorter than    1440 bp
  90% of sequence in fragments shorter than    1694 bp
  95% of sequence in fragments shorter than    2125 bp

# CviQI.HpaII (GTAC+CCGG) double digest, 7432 thousand fragments (5115078+2317722 sites), 400 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     404 bp
  30% of sequence in fragments shorter than     466 bp
  35% of sequence in fragments shorter than     526 bp
  40% of sequence in fragments shorter than     590 bp
  45% of sequence in fragments shorter than     657 bp
  50% of sequence in fragments shorter than     727 bp
  55% of sequence in fragments shorter than     803 bp
  60% of sequence in fragments shorter than     888 bp
  65% of sequence in fragments shorter than     979 bp
  70% of sequence in fragments shorter than    1082 bp
  75% of sequence in fragments shorter than    1204 bp
  80% of sequence in fragments shorter than    1342 bp
  85% of sequence in fragments shorter than    1516 bp
  90% of sequence in fragments shorter than    1775 bp
  95% of sequence in fragments shorter than    2235 bp

# BspKT6I.KasI (GATC+GGCGCC) double digest, 7429 thousand fragments (7199434+230381 sites), 401 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     323 bp
  25% of sequence in fragments shorter than     380 bp
  30% of sequence in fragments shorter than     438 bp
  35% of sequence in fragments shorter than     494 bp
  40% of sequence in fragments shorter than     557 bp
  45% of sequence in fragments shorter than     621 bp
  50% of sequence in fragments shorter than     689 bp
  55% of sequence in fragments shorter than     763 bp
  60% of sequence in fragments shorter than     844 bp
  65% of sequence in fragments shorter than     933 bp
  70% of sequence in fragments shorter than    1032 bp
  75% of sequence in fragments shorter than    1144 bp
  80% of sequence in fragments shorter than    1285 bp
  85% of sequence in fragments shorter than    1463 bp
  90% of sequence in fragments shorter than    1721 bp
  95% of sequence in fragments shorter than    2170 bp

# BspKT6I.SwaI (GATC+ATTTAAAT) double digest, 7425 thousand fragments (7199434+226602 sites), 401 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     192 bp
  15% of sequence in fragments shorter than     262 bp
  20% of sequence in fragments shorter than     321 bp
  25% of sequence in fragments shorter than     376 bp
  30% of sequence in fragments shorter than     433 bp
  35% of sequence in fragments shorter than     488 bp
  40% of sequence in fragments shorter than     549 bp
  45% of sequence in fragments shorter than     612 bp
  50% of sequence in fragments shorter than     678 bp
  55% of sequence in fragments shorter than     750 bp
  60% of sequence in fragments shorter than     829 bp
  65% of sequence in fragments shorter than     915 bp
  70% of sequence in fragments shorter than    1014 bp
  75% of sequence in fragments shorter than    1120 bp
  80% of sequence in fragments shorter than    1257 bp
  85% of sequence in fragments shorter than    1430 bp
  90% of sequence in fragments shorter than    1681 bp
  95% of sequence in fragments shorter than    2121 bp

# BamHI.Hin4II (GGATCC+CCTTC) double digest, 7425 thousand fragments (365999+7059915 sites), 401 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     218 bp
  15% of sequence in fragments shorter than     287 bp
  20% of sequence in fragments shorter than     349 bp
  25% of sequence in fragments shorter than     414 bp
  30% of sequence in fragments shorter than     481 bp
  35% of sequence in fragments shorter than     545 bp
  40% of sequence in fragments shorter than     617 bp
  45% of sequence in fragments shorter than     692 bp
  50% of sequence in fragments shorter than     774 bp
  55% of sequence in fragments shorter than     861 bp
  60% of sequence in fragments shorter than     957 bp
  65% of sequence in fragments shorter than    1058 bp
  70% of sequence in fragments shorter than    1182 bp
  75% of sequence in fragments shorter than    1324 bp
  80% of sequence in fragments shorter than    1493 bp
  85% of sequence in fragments shorter than    1713 bp
  90% of sequence in fragments shorter than    2014 bp
  95% of sequence in fragments shorter than    2534 bp

# BspNCI.SnaBI (CCAGA+TACGTA) double digest, 7414 thousand fragments (7282436+132299 sites), 401 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     336 bp
  25% of sequence in fragments shorter than     396 bp
  30% of sequence in fragments shorter than     457 bp
  35% of sequence in fragments shorter than     519 bp
  40% of sequence in fragments shorter than     582 bp
  45% of sequence in fragments shorter than     646 bp
  50% of sequence in fragments shorter than     717 bp
  55% of sequence in fragments shorter than     795 bp
  60% of sequence in fragments shorter than     878 bp
  65% of sequence in fragments shorter than     975 bp
  70% of sequence in fragments shorter than    1080 bp
  75% of sequence in fragments shorter than    1197 bp
  80% of sequence in fragments shorter than    1348 bp
  85% of sequence in fragments shorter than    1542 bp
  90% of sequence in fragments shorter than    1815 bp
  95% of sequence in fragments shorter than    2295 bp

# BspNCI.NaeI (CCAGA+GCCGGC) double digest, 7407 thousand fragments (7282436+125692 sites), 402 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     337 bp
  25% of sequence in fragments shorter than     397 bp
  30% of sequence in fragments shorter than     458 bp
  35% of sequence in fragments shorter than     521 bp
  40% of sequence in fragments shorter than     585 bp
  45% of sequence in fragments shorter than     651 bp
  50% of sequence in fragments shorter than     724 bp
  55% of sequence in fragments shorter than     802 bp
  60% of sequence in fragments shorter than     888 bp
  65% of sequence in fragments shorter than     987 bp
  70% of sequence in fragments shorter than    1094 bp
  75% of sequence in fragments shorter than    1214 bp
  80% of sequence in fragments shorter than    1369 bp
  85% of sequence in fragments shorter than    1568 bp
  90% of sequence in fragments shorter than    1849 bp
  95% of sequence in fragments shorter than    2341 bp

# BglII.BsmAI (AGATCT+GTCTC) double digest, 7405 thousand fragments (774732+6631585 sites), 402 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     267 bp
  20% of sequence in fragments shorter than     336 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     471 bp
  35% of sequence in fragments shorter than     537 bp
  40% of sequence in fragments shorter than     607 bp
  45% of sequence in fragments shorter than     682 bp
  50% of sequence in fragments shorter than     763 bp
  55% of sequence in fragments shorter than     851 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1056 bp
  70% of sequence in fragments shorter than    1179 bp
  75% of sequence in fragments shorter than    1324 bp
  80% of sequence in fragments shorter than    1503 bp
  85% of sequence in fragments shorter than    1736 bp
  90% of sequence in fragments shorter than    2068 bp
  95% of sequence in fragments shorter than    2673 bp

# BspNCI.XhoI (CCAGA+CTCGAG) double digest, 7403 thousand fragments (7282436+121308 sites), 402 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     275 bp
  20% of sequence in fragments shorter than     337 bp
  25% of sequence in fragments shorter than     396 bp
  30% of sequence in fragments shorter than     458 bp
  35% of sequence in fragments shorter than     520 bp
  40% of sequence in fragments shorter than     584 bp
  45% of sequence in fragments shorter than     648 bp
  50% of sequence in fragments shorter than     720 bp
  55% of sequence in fragments shorter than     798 bp
  60% of sequence in fragments shorter than     884 bp
  65% of sequence in fragments shorter than     982 bp
  70% of sequence in fragments shorter than    1090 bp
  75% of sequence in fragments shorter than    1208 bp
  80% of sequence in fragments shorter than    1362 bp
  85% of sequence in fragments shorter than    1559 bp
  90% of sequence in fragments shorter than    1837 bp
  95% of sequence in fragments shorter than    2325 bp

# BccI.BscGI (CCATC+CCCGT) double digest, 7401 thousand fragments (6170927+1231070 sites), 402 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     337 bp
  25% of sequence in fragments shorter than     390 bp
  30% of sequence in fragments shorter than     452 bp
  35% of sequence in fragments shorter than     517 bp
  40% of sequence in fragments shorter than     585 bp
  45% of sequence in fragments shorter than     655 bp
  50% of sequence in fragments shorter than     729 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     903 bp
  65% of sequence in fragments shorter than    1005 bp
  70% of sequence in fragments shorter than    1114 bp
  75% of sequence in fragments shorter than    1248 bp
  80% of sequence in fragments shorter than    1410 bp
  85% of sequence in fragments shorter than    1621 bp
  90% of sequence in fragments shorter than    1915 bp
  95% of sequence in fragments shorter than    2426 bp

# BstEII.Hin4II (GGTNACC+CCTTC) double digest, 7395 thousand fragments (335865+7059915 sites), 402 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     288 bp
  20% of sequence in fragments shorter than     350 bp
  25% of sequence in fragments shorter than     415 bp
  30% of sequence in fragments shorter than     483 bp
  35% of sequence in fragments shorter than     547 bp
  40% of sequence in fragments shorter than     620 bp
  45% of sequence in fragments shorter than     694 bp
  50% of sequence in fragments shorter than     776 bp
  55% of sequence in fragments shorter than     863 bp
  60% of sequence in fragments shorter than     960 bp
  65% of sequence in fragments shorter than    1062 bp
  70% of sequence in fragments shorter than    1185 bp
  75% of sequence in fragments shorter than    1327 bp
  80% of sequence in fragments shorter than    1497 bp
  85% of sequence in fragments shorter than    1717 bp
  90% of sequence in fragments shorter than    2018 bp
  95% of sequence in fragments shorter than    2536 bp

# BsmAI.NcoI (GTCTC+CCATGG) double digest, 7390 thousand fragments (6631585+759594 sites), 403 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     338 bp
  25% of sequence in fragments shorter than     405 bp
  30% of sequence in fragments shorter than     472 bp
  35% of sequence in fragments shorter than     539 bp
  40% of sequence in fragments shorter than     608 bp
  45% of sequence in fragments shorter than     684 bp
  50% of sequence in fragments shorter than     766 bp
  55% of sequence in fragments shorter than     855 bp
  60% of sequence in fragments shorter than     952 bp
  65% of sequence in fragments shorter than    1060 bp
  70% of sequence in fragments shorter than    1185 bp
  75% of sequence in fragments shorter than    1333 bp
  80% of sequence in fragments shorter than    1508 bp
  85% of sequence in fragments shorter than    1720 bp
  90% of sequence in fragments shorter than    2046 bp
  95% of sequence in fragments shorter than    2646 bp

# BsrI.PsiI (ACTGG+TTATAA) double digest, 7388 thousand fragments (5741310+1647912 sites), 403 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     273 bp
  20% of sequence in fragments shorter than     332 bp
  25% of sequence in fragments shorter than     391 bp
  30% of sequence in fragments shorter than     451 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     569 bp
  45% of sequence in fragments shorter than     633 bp
  50% of sequence in fragments shorter than     697 bp
  55% of sequence in fragments shorter than     766 bp
  60% of sequence in fragments shorter than     845 bp
  65% of sequence in fragments shorter than     930 bp
  70% of sequence in fragments shorter than    1029 bp
  75% of sequence in fragments shorter than    1141 bp
  80% of sequence in fragments shorter than    1280 bp
  85% of sequence in fragments shorter than    1456 bp
  90% of sequence in fragments shorter than    1709 bp
  95% of sequence in fragments shorter than    2159 bp

# ApoI.AvaI (RAATTY+CYCGRG) double digest, 7387 thousand fragments (6382433+1005714 sites), 403 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     269 bp
  20% of sequence in fragments shorter than     330 bp
  25% of sequence in fragments shorter than     388 bp
  30% of sequence in fragments shorter than     448 bp
  35% of sequence in fragments shorter than     512 bp
  40% of sequence in fragments shorter than     577 bp
  45% of sequence in fragments shorter than     646 bp
  50% of sequence in fragments shorter than     717 bp
  55% of sequence in fragments shorter than     797 bp
  60% of sequence in fragments shorter than     882 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1081 bp
  75% of sequence in fragments shorter than    1208 bp
  80% of sequence in fragments shorter than    1352 bp
  85% of sequence in fragments shorter than    1542 bp
  90% of sequence in fragments shorter than    1818 bp
  95% of sequence in fragments shorter than    2305 bp

# AclI.BspKT6I (AACGTT+GATC) double digest, 7371 thousand fragments (172706+7199434 sites), 404 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     324 bp
  25% of sequence in fragments shorter than     379 bp
  30% of sequence in fragments shorter than     437 bp
  35% of sequence in fragments shorter than     492 bp
  40% of sequence in fragments shorter than     554 bp
  45% of sequence in fragments shorter than     618 bp
  50% of sequence in fragments shorter than     685 bp
  55% of sequence in fragments shorter than     757 bp
  60% of sequence in fragments shorter than     837 bp
  65% of sequence in fragments shorter than     924 bp
  70% of sequence in fragments shorter than    1022 bp
  75% of sequence in fragments shorter than    1134 bp
  80% of sequence in fragments shorter than    1272 bp
  85% of sequence in fragments shorter than    1446 bp
  90% of sequence in fragments shorter than    1699 bp
  95% of sequence in fragments shorter than    2133 bp

# BclI.BsmAI (TGATCA+GTCTC) double digest, 7369 thousand fragments (738785+6631585 sites), 404 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     473 bp
  35% of sequence in fragments shorter than     540 bp
  40% of sequence in fragments shorter than     611 bp
  45% of sequence in fragments shorter than     685 bp
  50% of sequence in fragments shorter than     767 bp
  55% of sequence in fragments shorter than     855 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1055 bp
  70% of sequence in fragments shorter than    1177 bp
  75% of sequence in fragments shorter than    1319 bp
  80% of sequence in fragments shorter than    1497 bp
  85% of sequence in fragments shorter than    1725 bp
  90% of sequence in fragments shorter than    2049 bp
  95% of sequence in fragments shorter than    2624 bp

# BspNCI.ClaI (CCAGA+ATCGAT) double digest, 7368 thousand fragments (7282436+86477 sites), 404 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     276 bp
  20% of sequence in fragments shorter than     339 bp
  25% of sequence in fragments shorter than     398 bp
  30% of sequence in fragments shorter than     459 bp
  35% of sequence in fragments shorter than     522 bp
  40% of sequence in fragments shorter than     585 bp
  45% of sequence in fragments shorter than     652 bp
  50% of sequence in fragments shorter than     724 bp
  55% of sequence in fragments shorter than     802 bp
  60% of sequence in fragments shorter than     887 bp
  65% of sequence in fragments shorter than     985 bp
  70% of sequence in fragments shorter than    1091 bp
  75% of sequence in fragments shorter than    1210 bp
  80% of sequence in fragments shorter than    1363 bp
  85% of sequence in fragments shorter than    1559 bp
  90% of sequence in fragments shorter than    1837 bp
  95% of sequence in fragments shorter than    2321 bp

# BsmAI.FnuDII (GTCTC+CGCG) double digest, 7365 thousand fragments (6631585+733940 sites), 404 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     276 bp
  20% of sequence in fragments shorter than     353 bp
  25% of sequence in fragments shorter than     427 bp
  30% of sequence in fragments shorter than     503 bp
  35% of sequence in fragments shorter than     578 bp
  40% of sequence in fragments shorter than     655 bp
  45% of sequence in fragments shorter than     741 bp
  50% of sequence in fragments shorter than     834 bp
  55% of sequence in fragments shorter than     934 bp
  60% of sequence in fragments shorter than    1045 bp
  65% of sequence in fragments shorter than    1169 bp
  70% of sequence in fragments shorter than    1312 bp
  75% of sequence in fragments shorter than    1483 bp
  80% of sequence in fragments shorter than    1687 bp
  85% of sequence in fragments shorter than    1956 bp
  90% of sequence in fragments shorter than    2332 bp
  95% of sequence in fragments shorter than    3021 bp

# SspI.TfiI (AATATT+GAWTC) double digest, 7361 thousand fragments (2377267+4984362 sites), 404 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     330 bp
  25% of sequence in fragments shorter than     376 bp
  30% of sequence in fragments shorter than     437 bp
  35% of sequence in fragments shorter than     498 bp
  40% of sequence in fragments shorter than     562 bp
  45% of sequence in fragments shorter than     628 bp
  50% of sequence in fragments shorter than     693 bp
  55% of sequence in fragments shorter than     768 bp
  60% of sequence in fragments shorter than     849 bp
  65% of sequence in fragments shorter than     939 bp
  70% of sequence in fragments shorter than    1041 bp
  75% of sequence in fragments shorter than    1162 bp
  80% of sequence in fragments shorter than    1309 bp
  85% of sequence in fragments shorter than    1500 bp
  90% of sequence in fragments shorter than    1772 bp
  95% of sequence in fragments shorter than    2242 bp

# ApoI.BspHI (RAATTY+TCATGA) double digest, 7360 thousand fragments (6382433+978290 sites), 404 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     333 bp
  25% of sequence in fragments shorter than     395 bp
  30% of sequence in fragments shorter than     457 bp
  35% of sequence in fragments shorter than     525 bp
  40% of sequence in fragments shorter than     596 bp
  45% of sequence in fragments shorter than     670 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     837 bp
  60% of sequence in fragments shorter than     925 bp
  65% of sequence in fragments shorter than    1034 bp
  70% of sequence in fragments shorter than    1157 bp
  75% of sequence in fragments shorter than    1305 bp
  80% of sequence in fragments shorter than    1488 bp
  85% of sequence in fragments shorter than    1743 bp
  90% of sequence in fragments shorter than    2133 bp
  95% of sequence in fragments shorter than    2920 bp

# BspKT6I.PacI (GATC+TTAATTAA) double digest, 7358 thousand fragments (7199434+159455 sites), 404 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     324 bp
  25% of sequence in fragments shorter than     381 bp
  30% of sequence in fragments shorter than     438 bp
  35% of sequence in fragments shorter than     493 bp
  40% of sequence in fragments shorter than     556 bp
  45% of sequence in fragments shorter than     619 bp
  50% of sequence in fragments shorter than     687 bp
  55% of sequence in fragments shorter than     760 bp
  60% of sequence in fragments shorter than     841 bp
  65% of sequence in fragments shorter than     928 bp
  70% of sequence in fragments shorter than    1027 bp
  75% of sequence in fragments shorter than    1138 bp
  80% of sequence in fragments shorter than    1277 bp
  85% of sequence in fragments shorter than    1453 bp
  90% of sequence in fragments shorter than    1709 bp
  95% of sequence in fragments shorter than    2157 bp

# Hin4II.SexAI (CCTTC+ACCWGGT) double digest, 7357 thousand fragments (7059915+297900 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     288 bp
  20% of sequence in fragments shorter than     351 bp
  25% of sequence in fragments shorter than     415 bp
  30% of sequence in fragments shorter than     483 bp
  35% of sequence in fragments shorter than     547 bp
  40% of sequence in fragments shorter than     620 bp
  45% of sequence in fragments shorter than     694 bp
  50% of sequence in fragments shorter than     776 bp
  55% of sequence in fragments shorter than     863 bp
  60% of sequence in fragments shorter than     960 bp
  65% of sequence in fragments shorter than    1061 bp
  70% of sequence in fragments shorter than    1185 bp
  75% of sequence in fragments shorter than    1327 bp
  80% of sequence in fragments shorter than    1496 bp
  85% of sequence in fragments shorter than    1716 bp
  90% of sequence in fragments shorter than    2018 bp
  95% of sequence in fragments shorter than    2538 bp

# BspKT6I.Hpy99I (GATC+CGWCG) double digest, 7356 thousand fragments (7199434+157917 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     194 bp
  15% of sequence in fragments shorter than     264 bp
  20% of sequence in fragments shorter than     325 bp
  25% of sequence in fragments shorter than     381 bp
  30% of sequence in fragments shorter than     439 bp
  35% of sequence in fragments shorter than     494 bp
  40% of sequence in fragments shorter than     557 bp
  45% of sequence in fragments shorter than     621 bp
  50% of sequence in fragments shorter than     688 bp
  55% of sequence in fragments shorter than     762 bp
  60% of sequence in fragments shorter than     843 bp
  65% of sequence in fragments shorter than     931 bp
  70% of sequence in fragments shorter than    1030 bp
  75% of sequence in fragments shorter than    1142 bp
  80% of sequence in fragments shorter than    1282 bp
  85% of sequence in fragments shorter than    1459 bp
  90% of sequence in fragments shorter than    1716 bp
  95% of sequence in fragments shorter than    2160 bp

# AatII.BspNCI (GACGTC+CCAGA) double digest, 7353 thousand fragments (71089+7282436 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     524 bp
  40% of sequence in fragments shorter than     587 bp
  45% of sequence in fragments shorter than     655 bp
  50% of sequence in fragments shorter than     727 bp
  55% of sequence in fragments shorter than     806 bp
  60% of sequence in fragments shorter than     891 bp
  65% of sequence in fragments shorter than     990 bp
  70% of sequence in fragments shorter than    1097 bp
  75% of sequence in fragments shorter than    1216 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1570 bp
  90% of sequence in fragments shorter than    1850 bp
  95% of sequence in fragments shorter than    2341 bp

# BspNCI.SacII (CCAGA+CCGCGG) double digest, 7348 thousand fragments (7282436+66901 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     276 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     525 bp
  40% of sequence in fragments shorter than     588 bp
  45% of sequence in fragments shorter than     656 bp
  50% of sequence in fragments shorter than     727 bp
  55% of sequence in fragments shorter than     808 bp
  60% of sequence in fragments shorter than     894 bp
  65% of sequence in fragments shorter than     993 bp
  70% of sequence in fragments shorter than    1101 bp
  75% of sequence in fragments shorter than    1221 bp
  80% of sequence in fragments shorter than    1377 bp
  85% of sequence in fragments shorter than    1577 bp
  90% of sequence in fragments shorter than    1858 bp
  95% of sequence in fragments shorter than    2352 bp

# Hin4II.KpnI (CCTTC+GGTACC) double digest, 7347 thousand fragments (7059915+287974 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     220 bp
  15% of sequence in fragments shorter than     289 bp
  20% of sequence in fragments shorter than     350 bp
  25% of sequence in fragments shorter than     414 bp
  30% of sequence in fragments shorter than     483 bp
  35% of sequence in fragments shorter than     550 bp
  40% of sequence in fragments shorter than     624 bp
  45% of sequence in fragments shorter than     698 bp
  50% of sequence in fragments shorter than     780 bp
  55% of sequence in fragments shorter than     866 bp
  60% of sequence in fragments shorter than     963 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1189 bp
  75% of sequence in fragments shorter than    1331 bp
  80% of sequence in fragments shorter than    1501 bp
  85% of sequence in fragments shorter than    1722 bp
  90% of sequence in fragments shorter than    2025 bp
  95% of sequence in fragments shorter than    2547 bp

# Hin4II.NheI (CCTTC+GCTAGC) double digest, 7346 thousand fragments (7059915+287384 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     221 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     352 bp
  25% of sequence in fragments shorter than     417 bp
  30% of sequence in fragments shorter than     486 bp
  35% of sequence in fragments shorter than     550 bp
  40% of sequence in fragments shorter than     623 bp
  45% of sequence in fragments shorter than     698 bp
  50% of sequence in fragments shorter than     780 bp
  55% of sequence in fragments shorter than     866 bp
  60% of sequence in fragments shorter than     963 bp
  65% of sequence in fragments shorter than    1064 bp
  70% of sequence in fragments shorter than    1187 bp
  75% of sequence in fragments shorter than    1329 bp
  80% of sequence in fragments shorter than    1498 bp
  85% of sequence in fragments shorter than    1717 bp
  90% of sequence in fragments shorter than    2019 bp
  95% of sequence in fragments shorter than    2539 bp

# BsaAI.BsmAI (YACGTR+GTCTC) double digest, 7344 thousand fragments (713801+6631585 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     271 bp
  20% of sequence in fragments shorter than     341 bp
  25% of sequence in fragments shorter than     409 bp
  30% of sequence in fragments shorter than     478 bp
  35% of sequence in fragments shorter than     546 bp
  40% of sequence in fragments shorter than     616 bp
  45% of sequence in fragments shorter than     691 bp
  50% of sequence in fragments shorter than     773 bp
  55% of sequence in fragments shorter than     861 bp
  60% of sequence in fragments shorter than     957 bp
  65% of sequence in fragments shorter than    1063 bp
  70% of sequence in fragments shorter than    1188 bp
  75% of sequence in fragments shorter than    1334 bp
  80% of sequence in fragments shorter than    1498 bp
  85% of sequence in fragments shorter than    1723 bp
  90% of sequence in fragments shorter than    2037 bp
  95% of sequence in fragments shorter than    2593 bp

# BsrI.EsaBC3I (ACTGG+TCGA) double digest, 7344 thousand fragments (5741310+1603346 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     275 bp
  20% of sequence in fragments shorter than     333 bp
  25% of sequence in fragments shorter than     392 bp
  30% of sequence in fragments shorter than     450 bp
  35% of sequence in fragments shorter than     508 bp
  40% of sequence in fragments shorter than     569 bp
  45% of sequence in fragments shorter than     634 bp
  50% of sequence in fragments shorter than     700 bp
  55% of sequence in fragments shorter than     769 bp
  60% of sequence in fragments shorter than     849 bp
  65% of sequence in fragments shorter than     935 bp
  70% of sequence in fragments shorter than    1035 bp
  75% of sequence in fragments shorter than    1152 bp
  80% of sequence in fragments shorter than    1292 bp
  85% of sequence in fragments shorter than    1469 bp
  90% of sequence in fragments shorter than    1717 bp
  95% of sequence in fragments shorter than    2144 bp

# BspNCI.PspXI (CCAGA+VCTCGAGB) double digest, 7341 thousand fragments (7282436+59193 sites), 405 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     525 bp
  40% of sequence in fragments shorter than     588 bp
  45% of sequence in fragments shorter than     655 bp
  50% of sequence in fragments shorter than     727 bp
  55% of sequence in fragments shorter than     807 bp
  60% of sequence in fragments shorter than     893 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1099 bp
  75% of sequence in fragments shorter than    1218 bp
  80% of sequence in fragments shorter than    1374 bp
  85% of sequence in fragments shorter than    1572 bp
  90% of sequence in fragments shorter than    1853 bp
  95% of sequence in fragments shorter than    2343 bp

# BceAI.BsmAI (ACGGC+GTCTC) double digest, 7336 thousand fragments (705178+6631585 sites), 406 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     411 bp
  30% of sequence in fragments shorter than     481 bp
  35% of sequence in fragments shorter than     551 bp
  40% of sequence in fragments shorter than     623 bp
  45% of sequence in fragments shorter than     703 bp
  50% of sequence in fragments shorter than     789 bp
  55% of sequence in fragments shorter than     883 bp
  60% of sequence in fragments shorter than     987 bp
  65% of sequence in fragments shorter than    1103 bp
  70% of sequence in fragments shorter than    1236 bp
  75% of sequence in fragments shorter than    1396 bp
  80% of sequence in fragments shorter than    1588 bp
  85% of sequence in fragments shorter than    1844 bp
  90% of sequence in fragments shorter than    2199 bp
  95% of sequence in fragments shorter than    2846 bp

# AgeI.BspNCI (ACCGGT+CCAGA) double digest, 7335 thousand fragments (53386+7282436 sites), 406 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     525 bp
  40% of sequence in fragments shorter than     587 bp
  45% of sequence in fragments shorter than     656 bp
  50% of sequence in fragments shorter than     727 bp
  55% of sequence in fragments shorter than     807 bp
  60% of sequence in fragments shorter than     893 bp
  65% of sequence in fragments shorter than     992 bp
  70% of sequence in fragments shorter than    1099 bp
  75% of sequence in fragments shorter than    1219 bp
  80% of sequence in fragments shorter than    1374 bp
  85% of sequence in fragments shorter than    1573 bp
  90% of sequence in fragments shorter than    1853 bp
  95% of sequence in fragments shorter than    2345 bp

# BspKT6I.SnaBI (GATC+TACGTA) double digest, 7331 thousand fragments (7199434+132299 sites), 406 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     265 bp
  20% of sequence in fragments shorter than     325 bp
  25% of sequence in fragments shorter than     382 bp
  30% of sequence in fragments shorter than     440 bp
  35% of sequence in fragments shorter than     495 bp
  40% of sequence in fragments shorter than     557 bp
  45% of sequence in fragments shorter than     621 bp
  50% of sequence in fragments shorter than     689 bp
  55% of sequence in fragments shorter than     762 bp
  60% of sequence in fragments shorter than     843 bp
  65% of sequence in fragments shorter than     931 bp
  70% of sequence in fragments shorter than    1029 bp
  75% of sequence in fragments shorter than    1139 bp
  80% of sequence in fragments shorter than    1277 bp
  85% of sequence in fragments shorter than    1456 bp
  90% of sequence in fragments shorter than    1711 bp
  95% of sequence in fragments shorter than    2156 bp

# HphI.PstI (GGTGA+CTGCAG) double digest, 7328 thousand fragments (6007336+1321469 sites), 406 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     272 bp
  20% of sequence in fragments shorter than     334 bp
  25% of sequence in fragments shorter than     392 bp
  30% of sequence in fragments shorter than     454 bp
  35% of sequence in fragments shorter than     515 bp
  40% of sequence in fragments shorter than     580 bp
  45% of sequence in fragments shorter than     649 bp
  50% of sequence in fragments shorter than     722 bp
  55% of sequence in fragments shorter than     801 bp
  60% of sequence in fragments shorter than     886 bp
  65% of sequence in fragments shorter than     987 bp
  70% of sequence in fragments shorter than    1099 bp
  75% of sequence in fragments shorter than    1231 bp
  80% of sequence in fragments shorter than    1379 bp
  85% of sequence in fragments shorter than    1582 bp
  90% of sequence in fragments shorter than    1883 bp
  95% of sequence in fragments shorter than    2397 bp

# BciVI.BsmAI (GTATCC+GTCTC) double digest, 7327 thousand fragments (696093+6631585 sites), 406 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     271 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     475 bp
  35% of sequence in fragments shorter than     542 bp
  40% of sequence in fragments shorter than     612 bp
  45% of sequence in fragments shorter than     687 bp
  50% of sequence in fragments shorter than     768 bp
  55% of sequence in fragments shorter than     856 bp
  60% of sequence in fragments shorter than     952 bp
  65% of sequence in fragments shorter than    1059 bp
  70% of sequence in fragments shorter than    1183 bp
  75% of sequence in fragments shorter than    1329 bp
  80% of sequence in fragments shorter than    1506 bp
  85% of sequence in fragments shorter than    1732 bp
  90% of sequence in fragments shorter than    2049 bp
  95% of sequence in fragments shorter than    2608 bp

# BspKT6I.NaeI (GATC+GCCGGC) double digest, 7324 thousand fragments (7199434+125692 sites), 406 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     266 bp
  20% of sequence in fragments shorter than     326 bp
  25% of sequence in fragments shorter than     383 bp
  30% of sequence in fragments shorter than     441 bp
  35% of sequence in fragments shorter than     496 bp
  40% of sequence in fragments shorter than     559 bp
  45% of sequence in fragments shorter than     623 bp
  50% of sequence in fragments shorter than     691 bp
  55% of sequence in fragments shorter than     765 bp
  60% of sequence in fragments shorter than     846 bp
  65% of sequence in fragments shorter than     934 bp
  70% of sequence in fragments shorter than    1033 bp
  75% of sequence in fragments shorter than    1145 bp
  80% of sequence in fragments shorter than    1286 bp
  85% of sequence in fragments shorter than    1464 bp
  90% of sequence in fragments shorter than    1721 bp
  95% of sequence in fragments shorter than    2169 bp

# BspNCI.PmeI (CCAGA+GTTTAAAC) double digest, 7324 thousand fragments (7282436+42094 sites), 406 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     341 bp
  25% of sequence in fragments shorter than     401 bp
  30% of sequence in fragments shorter than     463 bp
  35% of sequence in fragments shorter than     526 bp
  40% of sequence in fragments shorter than     588 bp
  45% of sequence in fragments shorter than     656 bp
  50% of sequence in fragments shorter than     727 bp
  55% of sequence in fragments shorter than     807 bp
  60% of sequence in fragments shorter than     893 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1098 bp
  75% of sequence in fragments shorter than    1217 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1570 bp
  90% of sequence in fragments shorter than    1848 bp
  95% of sequence in fragments shorter than    2336 bp

# BspKT6I.XhoI (GATC+CTCGAG) double digest, 7320 thousand fragments (7199434+121308 sites), 407 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     195 bp
  15% of sequence in fragments shorter than     266 bp
  20% of sequence in fragments shorter than     326 bp
  25% of sequence in fragments shorter than     383 bp
  30% of sequence in fragments shorter than     442 bp
  35% of sequence in fragments shorter than     498 bp
  40% of sequence in fragments shorter than     561 bp
  45% of sequence in fragments shorter than     625 bp
  50% of sequence in fragments shorter than     693 bp
  55% of sequence in fragments shorter than     768 bp
  60% of sequence in fragments shorter than     849 bp
  65% of sequence in fragments shorter than     938 bp
  70% of sequence in fragments shorter than    1035 bp
  75% of sequence in fragments shorter than    1150 bp
  80% of sequence in fragments shorter than    1290 bp
  85% of sequence in fragments shorter than    1469 bp
  90% of sequence in fragments shorter than    1724 bp
  95% of sequence in fragments shorter than    2178 bp

# AciI.StyI (CCGC+CCWWGG) double digest, 7319 thousand fragments (4206130+3114107 sites), 407 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     215 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     387 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     558 bp
  35% of sequence in fragments shorter than     648 bp
  40% of sequence in fragments shorter than     740 bp
  45% of sequence in fragments shorter than     838 bp
  50% of sequence in fragments shorter than     948 bp
  55% of sequence in fragments shorter than    1067 bp
  60% of sequence in fragments shorter than    1200 bp
  65% of sequence in fragments shorter than    1351 bp
  70% of sequence in fragments shorter than    1516 bp
  75% of sequence in fragments shorter than    1721 bp
  80% of sequence in fragments shorter than    1969 bp
  85% of sequence in fragments shorter than    2291 bp
  90% of sequence in fragments shorter than    2749 bp
  95% of sequence in fragments shorter than    3529 bp

# ApoI.AvaIII (RAATTY+ATGCAT) double digest, 7315 thousand fragments (6382433+933357 sites), 407 bp average fragment length
   5% of sequence in fragments shorter than     133 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     334 bp
  25% of sequence in fragments shorter than     397 bp
  30% of sequence in fragments shorter than     463 bp
  35% of sequence in fragments shorter than     532 bp
  40% of sequence in fragments shorter than     602 bp
  45% of sequence in fragments shorter than     679 bp
  50% of sequence in fragments shorter than     759 bp
  55% of sequence in fragments shorter than     849 bp
  60% of sequence in fragments shorter than     943 bp
  65% of sequence in fragments shorter than    1053 bp
  70% of sequence in fragments shorter than    1182 bp
  75% of sequence in fragments shorter than    1328 bp
  80% of sequence in fragments shorter than    1516 bp
  85% of sequence in fragments shorter than    1777 bp
  90% of sequence in fragments shorter than    2178 bp
  95% of sequence in fragments shorter than    3005 bp

# BspNCI.SalI (CCAGA+GTCGAC) double digest, 7313 thousand fragments (7282436+31396 sites), 407 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     402 bp
  30% of sequence in fragments shorter than     464 bp
  35% of sequence in fragments shorter than     527 bp
  40% of sequence in fragments shorter than     589 bp
  45% of sequence in fragments shorter than     658 bp
  50% of sequence in fragments shorter than     728 bp
  55% of sequence in fragments shorter than     810 bp
  60% of sequence in fragments shorter than     896 bp
  65% of sequence in fragments shorter than     995 bp
  70% of sequence in fragments shorter than    1102 bp
  75% of sequence in fragments shorter than    1221 bp
  80% of sequence in fragments shorter than    1378 bp
  85% of sequence in fragments shorter than    1577 bp
  90% of sequence in fragments shorter than    1857 bp
  95% of sequence in fragments shorter than    2350 bp

# AccI.BthCI (GTMKAC+GCNGC) double digest, 7310 thousand fragments (1101063+6209929 sites), 407 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     457 bp
  30% of sequence in fragments shorter than     538 bp
  35% of sequence in fragments shorter than     620 bp
  40% of sequence in fragments shorter than     705 bp
  45% of sequence in fragments shorter than     797 bp
  50% of sequence in fragments shorter than     887 bp
  55% of sequence in fragments shorter than     990 bp
  60% of sequence in fragments shorter than    1100 bp
  65% of sequence in fragments shorter than    1226 bp
  70% of sequence in fragments shorter than    1370 bp
  75% of sequence in fragments shorter than    1534 bp
  80% of sequence in fragments shorter than    1728 bp
  85% of sequence in fragments shorter than    1980 bp
  90% of sequence in fragments shorter than    2324 bp
  95% of sequence in fragments shorter than    2930 bp

# AciI.NspI (CCGC+RCATGY) double digest, 7309 thousand fragments (4206130+3104359 sites), 407 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     222 bp
  15% of sequence in fragments shorter than     308 bp
  20% of sequence in fragments shorter than     386 bp
  25% of sequence in fragments shorter than     466 bp
  30% of sequence in fragments shorter than     545 bp
  35% of sequence in fragments shorter than     627 bp
  40% of sequence in fragments shorter than     711 bp
  45% of sequence in fragments shorter than     801 bp
  50% of sequence in fragments shorter than     892 bp
  55% of sequence in fragments shorter than     995 bp
  60% of sequence in fragments shorter than    1103 bp
  65% of sequence in fragments shorter than    1229 bp
  70% of sequence in fragments shorter than    1373 bp
  75% of sequence in fragments shorter than    1536 bp
  80% of sequence in fragments shorter than    1739 bp
  85% of sequence in fragments shorter than    1997 bp
  90% of sequence in fragments shorter than    2364 bp
  95% of sequence in fragments shorter than    2984 bp

# ApoI.StuI (RAATTY+AGGCCT) double digest, 7307 thousand fragments (6382433+925729 sites), 407 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     337 bp
  25% of sequence in fragments shorter than     397 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     527 bp
  40% of sequence in fragments shorter than     594 bp
  45% of sequence in fragments shorter than     665 bp
  50% of sequence in fragments shorter than     738 bp
  55% of sequence in fragments shorter than     823 bp
  60% of sequence in fragments shorter than     908 bp
  65% of sequence in fragments shorter than    1008 bp
  70% of sequence in fragments shorter than    1121 bp
  75% of sequence in fragments shorter than    1255 bp
  80% of sequence in fragments shorter than    1403 bp
  85% of sequence in fragments shorter than    1612 bp
  90% of sequence in fragments shorter than    1911 bp
  95% of sequence in fragments shorter than    2439 bp

# BspNCI.MluI (CCAGA+ACGCGT) double digest, 7303 thousand fragments (7282436+21415 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     402 bp
  30% of sequence in fragments shorter than     465 bp
  35% of sequence in fragments shorter than     527 bp
  40% of sequence in fragments shorter than     590 bp
  45% of sequence in fragments shorter than     658 bp
  50% of sequence in fragments shorter than     729 bp
  55% of sequence in fragments shorter than     810 bp
  60% of sequence in fragments shorter than     897 bp
  65% of sequence in fragments shorter than     996 bp
  70% of sequence in fragments shorter than    1103 bp
  75% of sequence in fragments shorter than    1223 bp
  80% of sequence in fragments shorter than    1379 bp
  85% of sequence in fragments shorter than    1579 bp
  90% of sequence in fragments shorter than    1860 bp
  95% of sequence in fragments shorter than    2353 bp

# HpaII.TfiI (CCGG+GAWTC) double digest, 7301 thousand fragments (2317722+4984362 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     282 bp
  20% of sequence in fragments shorter than     343 bp
  25% of sequence in fragments shorter than     397 bp
  30% of sequence in fragments shorter than     464 bp
  35% of sequence in fragments shorter than     530 bp
  40% of sequence in fragments shorter than     600 bp
  45% of sequence in fragments shorter than     672 bp
  50% of sequence in fragments shorter than     745 bp
  55% of sequence in fragments shorter than     824 bp
  60% of sequence in fragments shorter than     913 bp
  65% of sequence in fragments shorter than    1011 bp
  70% of sequence in fragments shorter than    1120 bp
  75% of sequence in fragments shorter than    1248 bp
  80% of sequence in fragments shorter than    1402 bp
  85% of sequence in fragments shorter than    1597 bp
  90% of sequence in fragments shorter than    1867 bp
  95% of sequence in fragments shorter than    2319 bp

# BspNCI.SgrAI (CCAGA+CRCCGGYG) double digest, 7297 thousand fragments (7282436+15270 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     402 bp
  30% of sequence in fragments shorter than     465 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     590 bp
  45% of sequence in fragments shorter than     659 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     811 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than     997 bp
  70% of sequence in fragments shorter than    1105 bp
  75% of sequence in fragments shorter than    1224 bp
  80% of sequence in fragments shorter than    1381 bp
  85% of sequence in fragments shorter than    1581 bp
  90% of sequence in fragments shorter than    1862 bp
  95% of sequence in fragments shorter than    2357 bp

# BspNCI.NruI (CCAGA+TCGCGA) double digest, 7296 thousand fragments (7282436+14872 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     465 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     590 bp
  45% of sequence in fragments shorter than     659 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     811 bp
  60% of sequence in fragments shorter than     897 bp
  65% of sequence in fragments shorter than     996 bp
  70% of sequence in fragments shorter than    1104 bp
  75% of sequence in fragments shorter than    1223 bp
  80% of sequence in fragments shorter than    1380 bp
  85% of sequence in fragments shorter than    1580 bp
  90% of sequence in fragments shorter than    1861 bp
  95% of sequence in fragments shorter than    2355 bp

# BspNCI.PvuI (CCAGA+CGATCG) double digest, 7296 thousand fragments (7282436+14186 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     465 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     659 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     811 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than     997 bp
  70% of sequence in fragments shorter than    1105 bp
  75% of sequence in fragments shorter than    1224 bp
  80% of sequence in fragments shorter than    1381 bp
  85% of sequence in fragments shorter than    1580 bp
  90% of sequence in fragments shorter than    1861 bp
  95% of sequence in fragments shorter than    2355 bp

# BspNCI.FseI (CCAGA+GGCCGGCC) double digest, 7295 thousand fragments (7282436+13662 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     465 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     659 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than     997 bp
  70% of sequence in fragments shorter than    1105 bp
  75% of sequence in fragments shorter than    1225 bp
  80% of sequence in fragments shorter than    1381 bp
  85% of sequence in fragments shorter than    1581 bp
  90% of sequence in fragments shorter than    1863 bp
  95% of sequence in fragments shorter than    2358 bp

# BsrBI.Hin4II (CCGCTC+CCTTC) double digest, 7294 thousand fragments (235523+7059915 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     222 bp
  15% of sequence in fragments shorter than     291 bp
  20% of sequence in fragments shorter than     355 bp
  25% of sequence in fragments shorter than     420 bp
  30% of sequence in fragments shorter than     489 bp
  35% of sequence in fragments shorter than     555 bp
  40% of sequence in fragments shorter than     630 bp
  45% of sequence in fragments shorter than     706 bp
  50% of sequence in fragments shorter than     790 bp
  55% of sequence in fragments shorter than     878 bp
  60% of sequence in fragments shorter than     978 bp
  65% of sequence in fragments shorter than    1082 bp
  70% of sequence in fragments shorter than    1207 bp
  75% of sequence in fragments shorter than    1353 bp
  80% of sequence in fragments shorter than    1528 bp
  85% of sequence in fragments shorter than    1750 bp
  90% of sequence in fragments shorter than    2060 bp
  95% of sequence in fragments shorter than    2591 bp

# BthCI.PpuMI (GCNGC+RGGWCCY) double digest, 7294 thousand fragments (6209929+1085138 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     131 bp
  10% of sequence in fragments shorter than     222 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     387 bp
  25% of sequence in fragments shorter than     474 bp
  30% of sequence in fragments shorter than     564 bp
  35% of sequence in fragments shorter than     658 bp
  40% of sequence in fragments shorter than     751 bp
  45% of sequence in fragments shorter than     853 bp
  50% of sequence in fragments shorter than     957 bp
  55% of sequence in fragments shorter than    1076 bp
  60% of sequence in fragments shorter than    1210 bp
  65% of sequence in fragments shorter than    1356 bp
  70% of sequence in fragments shorter than    1525 bp
  75% of sequence in fragments shorter than    1725 bp
  80% of sequence in fragments shorter than    1967 bp
  85% of sequence in fragments shorter than    2266 bp
  90% of sequence in fragments shorter than    2710 bp
  95% of sequence in fragments shorter than    3451 bp

# BthCI.PvuII (GCNGC+CAGCTG) double digest, 7293 thousand fragments (6209929+1084260 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     242 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     417 bp
  25% of sequence in fragments shorter than     509 bp
  30% of sequence in fragments shorter than     602 bp
  35% of sequence in fragments shorter than     697 bp
  40% of sequence in fragments shorter than     802 bp
  45% of sequence in fragments shorter than     903 bp
  50% of sequence in fragments shorter than    1020 bp
  55% of sequence in fragments shorter than    1142 bp
  60% of sequence in fragments shorter than    1278 bp
  65% of sequence in fragments shorter than    1431 bp
  70% of sequence in fragments shorter than    1609 bp
  75% of sequence in fragments shorter than    1805 bp
  80% of sequence in fragments shorter than    2050 bp
  85% of sequence in fragments shorter than    2359 bp
  90% of sequence in fragments shorter than    2810 bp
  95% of sequence in fragments shorter than    3578 bp

# BspNCI.NotI (CCAGA+GCGGCCGC) double digest, 7291 thousand fragments (7282436+9795 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     465 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     659 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than     997 bp
  70% of sequence in fragments shorter than    1105 bp
  75% of sequence in fragments shorter than    1225 bp
  80% of sequence in fragments shorter than    1382 bp
  85% of sequence in fragments shorter than    1582 bp
  90% of sequence in fragments shorter than    1863 bp
  95% of sequence in fragments shorter than    2359 bp

# BspNCI.RsrII (CCAGA+CGGWCCG) double digest, 7291 thousand fragments (7282436+9764 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     465 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     660 bp
  50% of sequence in fragments shorter than     730 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than     997 bp
  70% of sequence in fragments shorter than    1105 bp
  75% of sequence in fragments shorter than    1225 bp
  80% of sequence in fragments shorter than    1382 bp
  85% of sequence in fragments shorter than    1582 bp
  90% of sequence in fragments shorter than    1863 bp
  95% of sequence in fragments shorter than    2358 bp

# BsrI.HgiCI (ACTGG+GGYRCC) double digest, 7291 thousand fragments (5741310+1550877 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     338 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     460 bp
  35% of sequence in fragments shorter than     523 bp
  40% of sequence in fragments shorter than     590 bp
  45% of sequence in fragments shorter than     658 bp
  50% of sequence in fragments shorter than     722 bp
  55% of sequence in fragments shorter than     802 bp
  60% of sequence in fragments shorter than     888 bp
  65% of sequence in fragments shorter than     985 bp
  70% of sequence in fragments shorter than    1094 bp
  75% of sequence in fragments shorter than    1223 bp
  80% of sequence in fragments shorter than    1380 bp
  85% of sequence in fragments shorter than    1580 bp
  90% of sequence in fragments shorter than    1873 bp
  95% of sequence in fragments shorter than    2393 bp

# BsmI.BsrI (GAATGC+ACTGG) double digest, 7290 thousand fragments (1549350+5741310 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     336 bp
  25% of sequence in fragments shorter than     398 bp
  30% of sequence in fragments shorter than     458 bp
  35% of sequence in fragments shorter than     522 bp
  40% of sequence in fragments shorter than     588 bp
  45% of sequence in fragments shorter than     656 bp
  50% of sequence in fragments shorter than     725 bp
  55% of sequence in fragments shorter than     803 bp
  60% of sequence in fragments shorter than     886 bp
  65% of sequence in fragments shorter than     981 bp
  70% of sequence in fragments shorter than    1085 bp
  75% of sequence in fragments shorter than    1209 bp
  80% of sequence in fragments shorter than    1357 bp
  85% of sequence in fragments shorter than    1540 bp
  90% of sequence in fragments shorter than    1797 bp
  95% of sequence in fragments shorter than    2232 bp

# Hin4II.KasI (CCTTC+GGCGCC) double digest, 7289 thousand fragments (7059915+230381 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     291 bp
  20% of sequence in fragments shorter than     353 bp
  25% of sequence in fragments shorter than     417 bp
  30% of sequence in fragments shorter than     485 bp
  35% of sequence in fragments shorter than     549 bp
  40% of sequence in fragments shorter than     622 bp
  45% of sequence in fragments shorter than     696 bp
  50% of sequence in fragments shorter than     778 bp
  55% of sequence in fragments shorter than     864 bp
  60% of sequence in fragments shorter than     960 bp
  65% of sequence in fragments shorter than    1062 bp
  70% of sequence in fragments shorter than    1184 bp
  75% of sequence in fragments shorter than    1325 bp
  80% of sequence in fragments shorter than    1493 bp
  85% of sequence in fragments shorter than    1712 bp
  90% of sequence in fragments shorter than    2011 bp
  95% of sequence in fragments shorter than    2525 bp

# AscI.BspNCI (GGCGCGCC+CCAGA) double digest, 7286 thousand fragments (4751+7282436 sites), 408 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     343 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     466 bp
  35% of sequence in fragments shorter than     529 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     660 bp
  50% of sequence in fragments shorter than     731 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     899 bp
  65% of sequence in fragments shorter than     998 bp
  70% of sequence in fragments shorter than    1106 bp
  75% of sequence in fragments shorter than    1225 bp
  80% of sequence in fragments shorter than    1382 bp
  85% of sequence in fragments shorter than    1582 bp
  90% of sequence in fragments shorter than    1864 bp
  95% of sequence in fragments shorter than    2359 bp

# Hin4II.SwaI (CCTTC+ATTTAAAT) double digest, 7286 thousand fragments (7059915+226602 sites), 409 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     291 bp
  20% of sequence in fragments shorter than     354 bp
  25% of sequence in fragments shorter than     418 bp
  30% of sequence in fragments shorter than     486 bp
  35% of sequence in fragments shorter than     550 bp
  40% of sequence in fragments shorter than     622 bp
  45% of sequence in fragments shorter than     696 bp
  50% of sequence in fragments shorter than     777 bp
  55% of sequence in fragments shorter than     862 bp
  60% of sequence in fragments shorter than     956 bp
  65% of sequence in fragments shorter than    1056 bp
  70% of sequence in fragments shorter than    1176 bp
  75% of sequence in fragments shorter than    1315 bp
  80% of sequence in fragments shorter than    1479 bp
  85% of sequence in fragments shorter than    1693 bp
  90% of sequence in fragments shorter than    1986 bp
  95% of sequence in fragments shorter than    2491 bp

# BspKT6I.ClaI (GATC+ATCGAT) double digest, 7285 thousand fragments (7199434+86477 sites), 409 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     386 bp
  30% of sequence in fragments shorter than     445 bp
  35% of sequence in fragments shorter than     501 bp
  40% of sequence in fragments shorter than     564 bp
  45% of sequence in fragments shorter than     628 bp
  50% of sequence in fragments shorter than     697 bp
  55% of sequence in fragments shorter than     772 bp
  60% of sequence in fragments shorter than     854 bp
  65% of sequence in fragments shorter than     943 bp
  70% of sequence in fragments shorter than    1040 bp
  75% of sequence in fragments shorter than    1155 bp
  80% of sequence in fragments shorter than    1297 bp
  85% of sequence in fragments shorter than    1476 bp
  90% of sequence in fragments shorter than    1732 bp
  95% of sequence in fragments shorter than    2192 bp

# ApoI.NdeI (RAATTY+CATATG) double digest, 7285 thousand fragments (6382433+903360 sites), 409 bp average fragment length
   5% of sequence in fragments shorter than     134 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     273 bp
  20% of sequence in fragments shorter than     333 bp
  25% of sequence in fragments shorter than     396 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     531 bp
  40% of sequence in fragments shorter than     603 bp
  45% of sequence in fragments shorter than     679 bp
  50% of sequence in fragments shorter than     756 bp
  55% of sequence in fragments shorter than     841 bp
  60% of sequence in fragments shorter than     937 bp
  65% of sequence in fragments shorter than    1051 bp
  70% of sequence in fragments shorter than    1185 bp
  75% of sequence in fragments shorter than    1346 bp
  80% of sequence in fragments shorter than    1545 bp
  85% of sequence in fragments shorter than    1825 bp
  90% of sequence in fragments shorter than    2245 bp
  95% of sequence in fragments shorter than    3121 bp

# AflII.BsmAI (CTTAAG+GTCTC) double digest, 7274 thousand fragments (643284+6631585 sites), 409 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     273 bp
  20% of sequence in fragments shorter than     344 bp
  25% of sequence in fragments shorter than     412 bp
  30% of sequence in fragments shorter than     481 bp
  35% of sequence in fragments shorter than     549 bp
  40% of sequence in fragments shorter than     619 bp
  45% of sequence in fragments shorter than     696 bp
  50% of sequence in fragments shorter than     779 bp
  55% of sequence in fragments shorter than     869 bp
  60% of sequence in fragments shorter than     968 bp
  65% of sequence in fragments shorter than    1078 bp
  70% of sequence in fragments shorter than    1205 bp
  75% of sequence in fragments shorter than    1354 bp
  80% of sequence in fragments shorter than    1536 bp
  85% of sequence in fragments shorter than    1774 bp
  90% of sequence in fragments shorter than    2109 bp
  95% of sequence in fragments shorter than    2720 bp

# AccI.BccI (GTMKAC+CCATC) double digest, 7271 thousand fragments (1101063+6170927 sites), 409 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     215 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     339 bp
  25% of sequence in fragments shorter than     399 bp
  30% of sequence in fragments shorter than     457 bp
  35% of sequence in fragments shorter than     518 bp
  40% of sequence in fragments shorter than     583 bp
  45% of sequence in fragments shorter than     650 bp
  50% of sequence in fragments shorter than     721 bp
  55% of sequence in fragments shorter than     798 bp
  60% of sequence in fragments shorter than     882 bp
  65% of sequence in fragments shorter than     977 bp
  70% of sequence in fragments shorter than    1079 bp
  75% of sequence in fragments shorter than    1201 bp
  80% of sequence in fragments shorter than    1348 bp
  85% of sequence in fragments shorter than    1536 bp
  90% of sequence in fragments shorter than    1807 bp
  95% of sequence in fragments shorter than    2294 bp

# AatII.BspKT6I (GACGTC+GATC) double digest, 7270 thousand fragments (71089+7199434 sites), 409 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     386 bp
  30% of sequence in fragments shorter than     444 bp
  35% of sequence in fragments shorter than     500 bp
  40% of sequence in fragments shorter than     563 bp
  45% of sequence in fragments shorter than     628 bp
  50% of sequence in fragments shorter than     696 bp
  55% of sequence in fragments shorter than     771 bp
  60% of sequence in fragments shorter than     853 bp
  65% of sequence in fragments shorter than     941 bp
  70% of sequence in fragments shorter than    1039 bp
  75% of sequence in fragments shorter than    1154 bp
  80% of sequence in fragments shorter than    1295 bp
  85% of sequence in fragments shorter than    1475 bp
  90% of sequence in fragments shorter than    1730 bp
  95% of sequence in fragments shorter than    2187 bp

# BspKT6I.SacII (GATC+CCGCGG) double digest, 7265 thousand fragments (7199434+66901 sites), 410 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     196 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     328 bp
  25% of sequence in fragments shorter than     386 bp
  30% of sequence in fragments shorter than     445 bp
  35% of sequence in fragments shorter than     500 bp
  40% of sequence in fragments shorter than     564 bp
  45% of sequence in fragments shorter than     628 bp
  50% of sequence in fragments shorter than     696 bp
  55% of sequence in fragments shorter than     771 bp
  60% of sequence in fragments shorter than     853 bp
  65% of sequence in fragments shorter than     942 bp
  70% of sequence in fragments shorter than    1040 bp
  75% of sequence in fragments shorter than    1155 bp
  80% of sequence in fragments shorter than    1296 bp
  85% of sequence in fragments shorter than    1476 bp
  90% of sequence in fragments shorter than    1731 bp
  95% of sequence in fragments shorter than    2189 bp

# BsmAI.HaeII (GTCTC+RGCGCY) double digest, 7261 thousand fragments (6631585+630101 sites), 410 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     419 bp
  30% of sequence in fragments shorter than     492 bp
  35% of sequence in fragments shorter than     564 bp
  40% of sequence in fragments shorter than     640 bp
  45% of sequence in fragments shorter than     717 bp
  50% of sequence in fragments shorter than     807 bp
  55% of sequence in fragments shorter than     902 bp
  60% of sequence in fragments shorter than    1009 bp
  65% of sequence in fragments shorter than    1125 bp
  70% of sequence in fragments shorter than    1261 bp
  75% of sequence in fragments shorter than    1422 bp
  80% of sequence in fragments shorter than    1616 bp
  85% of sequence in fragments shorter than    1872 bp
  90% of sequence in fragments shorter than    2212 bp
  95% of sequence in fragments shorter than    2808 bp

# BspKT6I.PspXI (GATC+VCTCGAGB) double digest, 7258 thousand fragments (7199434+59193 sites), 410 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     329 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     445 bp
  35% of sequence in fragments shorter than     501 bp
  40% of sequence in fragments shorter than     564 bp
  45% of sequence in fragments shorter than     629 bp
  50% of sequence in fragments shorter than     697 bp
  55% of sequence in fragments shorter than     772 bp
  60% of sequence in fragments shorter than     854 bp
  65% of sequence in fragments shorter than     944 bp
  70% of sequence in fragments shorter than    1041 bp
  75% of sequence in fragments shorter than    1157 bp
  80% of sequence in fragments shorter than    1298 bp
  85% of sequence in fragments shorter than    1478 bp
  90% of sequence in fragments shorter than    1733 bp
  95% of sequence in fragments shorter than    2191 bp

# BccI.PpuMI (CCATC+RGGWCCY) double digest, 7255 thousand fragments (6170927+1085138 sites), 410 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     339 bp
  25% of sequence in fragments shorter than     402 bp
  30% of sequence in fragments shorter than     463 bp
  35% of sequence in fragments shorter than     527 bp
  40% of sequence in fragments shorter than     598 bp
  45% of sequence in fragments shorter than     670 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     833 bp
  60% of sequence in fragments shorter than     925 bp
  65% of sequence in fragments shorter than    1030 bp
  70% of sequence in fragments shorter than    1144 bp
  75% of sequence in fragments shorter than    1283 bp
  80% of sequence in fragments shorter than    1455 bp
  85% of sequence in fragments shorter than    1681 bp
  90% of sequence in fragments shorter than    2008 bp
  95% of sequence in fragments shorter than    2622 bp

# BccI.PvuII (CCATC+CAGCTG) double digest, 7254 thousand fragments (6170927+1084260 sites), 410 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     402 bp
  30% of sequence in fragments shorter than     463 bp
  35% of sequence in fragments shorter than     528 bp
  40% of sequence in fragments shorter than     597 bp
  45% of sequence in fragments shorter than     666 bp
  50% of sequence in fragments shorter than     743 bp
  55% of sequence in fragments shorter than     827 bp
  60% of sequence in fragments shorter than     917 bp
  65% of sequence in fragments shorter than    1020 bp
  70% of sequence in fragments shorter than    1131 bp
  75% of sequence in fragments shorter than    1267 bp
  80% of sequence in fragments shorter than    1434 bp
  85% of sequence in fragments shorter than    1654 bp
  90% of sequence in fragments shorter than    1974 bp
  95% of sequence in fragments shorter than    2575 bp

# AgeI.BspKT6I (ACCGGT+GATC) double digest, 7252 thousand fragments (53386+7199434 sites), 410 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     268 bp
  20% of sequence in fragments shorter than     329 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     445 bp
  35% of sequence in fragments shorter than     502 bp
  40% of sequence in fragments shorter than     565 bp
  45% of sequence in fragments shorter than     629 bp
  50% of sequence in fragments shorter than     698 bp
  55% of sequence in fragments shorter than     773 bp
  60% of sequence in fragments shorter than     855 bp
  65% of sequence in fragments shorter than     944 bp
  70% of sequence in fragments shorter than    1042 bp
  75% of sequence in fragments shorter than    1158 bp
  80% of sequence in fragments shorter than    1299 bp
  85% of sequence in fragments shorter than    1480 bp
  90% of sequence in fragments shorter than    1735 bp
  95% of sequence in fragments shorter than    2195 bp

# ApoI.HindIII (RAATTY+AAGCTT) double digest, 7242 thousand fragments (6382433+860365 sites), 411 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     337 bp
  25% of sequence in fragments shorter than     401 bp
  30% of sequence in fragments shorter than     467 bp
  35% of sequence in fragments shorter than     537 bp
  40% of sequence in fragments shorter than     608 bp
  45% of sequence in fragments shorter than     684 bp
  50% of sequence in fragments shorter than     766 bp
  55% of sequence in fragments shorter than     856 bp
  60% of sequence in fragments shorter than     951 bp
  65% of sequence in fragments shorter than    1061 bp
  70% of sequence in fragments shorter than    1191 bp
  75% of sequence in fragments shorter than    1336 bp
  80% of sequence in fragments shorter than    1520 bp
  85% of sequence in fragments shorter than    1778 bp
  90% of sequence in fragments shorter than    2164 bp
  95% of sequence in fragments shorter than    2971 bp

# BspKT6I.PmeI (GATC+GTTTAAAC) double digest, 7241 thousand fragments (7199434+42094 sites), 411 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     269 bp
  20% of sequence in fragments shorter than     329 bp
  25% of sequence in fragments shorter than     387 bp
  30% of sequence in fragments shorter than     446 bp
  35% of sequence in fragments shorter than     502 bp
  40% of sequence in fragments shorter than     565 bp
  45% of sequence in fragments shorter than     630 bp
  50% of sequence in fragments shorter than     698 bp
  55% of sequence in fragments shorter than     773 bp
  60% of sequence in fragments shorter than     856 bp
  65% of sequence in fragments shorter than     945 bp
  70% of sequence in fragments shorter than    1042 bp
  75% of sequence in fragments shorter than    1158 bp
  80% of sequence in fragments shorter than    1299 bp
  85% of sequence in fragments shorter than    1480 bp
  90% of sequence in fragments shorter than    1735 bp
  95% of sequence in fragments shorter than    2193 bp

# BscGI.HphI (CCCGT+GGTGA) double digest, 7237 thousand fragments (1231070+6007336 sites), 411 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     280 bp
  20% of sequence in fragments shorter than     344 bp
  25% of sequence in fragments shorter than     401 bp
  30% of sequence in fragments shorter than     466 bp
  35% of sequence in fragments shorter than     529 bp
  40% of sequence in fragments shorter than     597 bp
  45% of sequence in fragments shorter than     670 bp
  50% of sequence in fragments shorter than     746 bp
  55% of sequence in fragments shorter than     827 bp
  60% of sequence in fragments shorter than     919 bp
  65% of sequence in fragments shorter than    1024 bp
  70% of sequence in fragments shorter than    1142 bp
  75% of sequence in fragments shorter than    1279 bp
  80% of sequence in fragments shorter than    1437 bp
  85% of sequence in fragments shorter than    1651 bp
  90% of sequence in fragments shorter than    1952 bp
  95% of sequence in fragments shorter than    2456 bp

# BsmAI.SacI (GTCTC+GAGCTC) double digest, 7234 thousand fragments (6631585+603323 sites), 411 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     274 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     415 bp
  30% of sequence in fragments shorter than     486 bp
  35% of sequence in fragments shorter than     556 bp
  40% of sequence in fragments shorter than     628 bp
  45% of sequence in fragments shorter than     708 bp
  50% of sequence in fragments shorter than     795 bp
  55% of sequence in fragments shorter than     889 bp
  60% of sequence in fragments shorter than     993 bp
  65% of sequence in fragments shorter than    1108 bp
  70% of sequence in fragments shorter than    1239 bp
  75% of sequence in fragments shorter than    1399 bp
  80% of sequence in fragments shorter than    1594 bp
  85% of sequence in fragments shorter than    1854 bp
  90% of sequence in fragments shorter than    2201 bp
  95% of sequence in fragments shorter than    2847 bp

# ApoI.XbaI (RAATTY+TCTAGA) double digest, 7232 thousand fragments (6382433+850998 sites), 412 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     339 bp
  25% of sequence in fragments shorter than     400 bp
  30% of sequence in fragments shorter than     468 bp
  35% of sequence in fragments shorter than     538 bp
  40% of sequence in fragments shorter than     609 bp
  45% of sequence in fragments shorter than     684 bp
  50% of sequence in fragments shorter than     763 bp
  55% of sequence in fragments shorter than     853 bp
  60% of sequence in fragments shorter than     947 bp
  65% of sequence in fragments shorter than    1053 bp
  70% of sequence in fragments shorter than    1182 bp
  75% of sequence in fragments shorter than    1333 bp
  80% of sequence in fragments shorter than    1523 bp
  85% of sequence in fragments shorter than    1779 bp
  90% of sequence in fragments shorter than    2162 bp
  95% of sequence in fragments shorter than    2935 bp

# AclI.Hin4II (AACGTT+CCTTC) double digest, 7232 thousand fragments (172706+7059915 sites), 412 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     224 bp
  15% of sequence in fragments shorter than     293 bp
  20% of sequence in fragments shorter than     358 bp
  25% of sequence in fragments shorter than     423 bp
  30% of sequence in fragments shorter than     492 bp
  35% of sequence in fragments shorter than     557 bp
  40% of sequence in fragments shorter than     631 bp
  45% of sequence in fragments shorter than     706 bp
  50% of sequence in fragments shorter than     789 bp
  55% of sequence in fragments shorter than     876 bp
  60% of sequence in fragments shorter than     973 bp
  65% of sequence in fragments shorter than    1077 bp
  70% of sequence in fragments shorter than    1200 bp
  75% of sequence in fragments shorter than    1343 bp
  80% of sequence in fragments shorter than    1514 bp
  85% of sequence in fragments shorter than    1733 bp
  90% of sequence in fragments shorter than    2037 bp
  95% of sequence in fragments shorter than    2559 bp

# BspKT6I.SalI (GATC+GTCGAC) double digest, 7230 thousand fragments (7199434+31396 sites), 412 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     197 bp
  15% of sequence in fragments shorter than     269 bp
  20% of sequence in fragments shorter than     330 bp
  25% of sequence in fragments shorter than     388 bp
  30% of sequence in fragments shorter than     447 bp
  35% of sequence in fragments shorter than     503 bp
  40% of sequence in fragments shorter than     566 bp
  45% of sequence in fragments shorter than     631 bp
  50% of sequence in fragments shorter than     700 bp
  55% of sequence in fragments shorter than     775 bp
  60% of sequence in fragments shorter than     858 bp
  65% of sequence in fragments shorter than     947 bp
  70% of sequence in fragments shorter than    1045 bp
  75% of sequence in fragments shorter than    1161 bp
  80% of sequence in fragments shorter than    1303 bp
  85% of sequence in fragments shorter than    1484 bp
  90% of sequence in fragments shorter than    1740 bp
  95% of sequence in fragments shorter than    2202 bp

# AflIII.BsrI (ACRYGT+ACTGG) double digest, 7226 thousand fragments (1485394+5741310 sites), 412 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     280 bp
  20% of sequence in fragments shorter than     340 bp
  25% of sequence in fragments shorter than     402 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     525 bp
  40% of sequence in fragments shorter than     591 bp
  45% of sequence in fragments shorter than     659 bp
  50% of sequence in fragments shorter than     723 bp
  55% of sequence in fragments shorter than     800 bp
  60% of sequence in fragments shorter than     880 bp
  65% of sequence in fragments shorter than     974 bp
  70% of sequence in fragments shorter than    1076 bp
  75% of sequence in fragments shorter than    1197 bp
  80% of sequence in fragments shorter than    1345 bp
  85% of sequence in fragments shorter than    1533 bp
  90% of sequence in fragments shorter than    1793 bp
  95% of sequence in fragments shorter than    2250 bp

# AvrII.BsmAI (CCTAGG+GTCTC) double digest, 7226 thousand fragments (594956+6631585 sites), 412 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     276 bp
  20% of sequence in fragments shorter than     349 bp
  25% of sequence in fragments shorter than     419 bp
  30% of sequence in fragments shorter than     490 bp
  35% of sequence in fragments shorter than     561 bp
  40% of sequence in fragments shorter than     632 bp
  45% of sequence in fragments shorter than     709 bp
  50% of sequence in fragments shorter than     793 bp
  55% of sequence in fragments shorter than     886 bp
  60% of sequence in fragments shorter than     989 bp
  65% of sequence in fragments shorter than    1103 bp
  70% of sequence in fragments shorter than    1232 bp
  75% of sequence in fragments shorter than    1384 bp
  80% of sequence in fragments shorter than    1567 bp
  85% of sequence in fragments shorter than    1803 bp
  90% of sequence in fragments shorter than    2143 bp
  95% of sequence in fragments shorter than    2782 bp

# BspKT6I.MluI (GATC+ACGCGT) double digest, 7220 thousand fragments (7199434+21415 sites), 412 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     330 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     447 bp
  35% of sequence in fragments shorter than     504 bp
  40% of sequence in fragments shorter than     567 bp
  45% of sequence in fragments shorter than     632 bp
  50% of sequence in fragments shorter than     701 bp
  55% of sequence in fragments shorter than     776 bp
  60% of sequence in fragments shorter than     859 bp
  65% of sequence in fragments shorter than     949 bp
  70% of sequence in fragments shorter than    1046 bp
  75% of sequence in fragments shorter than    1163 bp
  80% of sequence in fragments shorter than    1304 bp
  85% of sequence in fragments shorter than    1486 bp
  90% of sequence in fragments shorter than    1743 bp
  95% of sequence in fragments shorter than    2205 bp

# Hin4II.PacI (CCTTC+TTAATTAA) double digest, 7218 thousand fragments (7059915+159455 sites), 412 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     294 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     423 bp
  30% of sequence in fragments shorter than     493 bp
  35% of sequence in fragments shorter than     557 bp
  40% of sequence in fragments shorter than     631 bp
  45% of sequence in fragments shorter than     706 bp
  50% of sequence in fragments shorter than     788 bp
  55% of sequence in fragments shorter than     874 bp
  60% of sequence in fragments shorter than     972 bp
  65% of sequence in fragments shorter than    1074 bp
  70% of sequence in fragments shorter than    1197 bp
  75% of sequence in fragments shorter than    1339 bp
  80% of sequence in fragments shorter than    1507 bp
  85% of sequence in fragments shorter than    1725 bp
  90% of sequence in fragments shorter than    2026 bp
  95% of sequence in fragments shorter than    2542 bp

# Hin4II.Hpy99I (CCTTC+CGWCG) double digest, 7217 thousand fragments (7059915+157917 sites), 412 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     224 bp
  15% of sequence in fragments shorter than     294 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     424 bp
  30% of sequence in fragments shorter than     495 bp
  35% of sequence in fragments shorter than     560 bp
  40% of sequence in fragments shorter than     635 bp
  45% of sequence in fragments shorter than     712 bp
  50% of sequence in fragments shorter than     795 bp
  55% of sequence in fragments shorter than     884 bp
  60% of sequence in fragments shorter than     984 bp
  65% of sequence in fragments shorter than    1090 bp
  70% of sequence in fragments shorter than    1215 bp
  75% of sequence in fragments shorter than    1362 bp
  80% of sequence in fragments shorter than    1537 bp
  85% of sequence in fragments shorter than    1761 bp
  90% of sequence in fragments shorter than    2071 bp
  95% of sequence in fragments shorter than    2606 bp

# AvaI.BthCI (CYCGRG+GCNGC) double digest, 7215 thousand fragments (1005714+6209929 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     313 bp
  20% of sequence in fragments shorter than     395 bp
  25% of sequence in fragments shorter than     480 bp
  30% of sequence in fragments shorter than     568 bp
  35% of sequence in fragments shorter than     660 bp
  40% of sequence in fragments shorter than     756 bp
  45% of sequence in fragments shorter than     858 bp
  50% of sequence in fragments shorter than     966 bp
  55% of sequence in fragments shorter than    1083 bp
  60% of sequence in fragments shorter than    1216 bp
  65% of sequence in fragments shorter than    1367 bp
  70% of sequence in fragments shorter than    1538 bp
  75% of sequence in fragments shorter than    1731 bp
  80% of sequence in fragments shorter than    1974 bp
  85% of sequence in fragments shorter than    2275 bp
  90% of sequence in fragments shorter than    2725 bp
  95% of sequence in fragments shorter than    3485 bp

# BspKT6I.SgrAI (GATC+CRCCGGYG) double digest, 7214 thousand fragments (7199434+15270 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     330 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     448 bp
  35% of sequence in fragments shorter than     504 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     632 bp
  50% of sequence in fragments shorter than     701 bp
  55% of sequence in fragments shorter than     777 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     949 bp
  70% of sequence in fragments shorter than    1047 bp
  75% of sequence in fragments shorter than    1163 bp
  80% of sequence in fragments shorter than    1305 bp
  85% of sequence in fragments shorter than    1487 bp
  90% of sequence in fragments shorter than    1744 bp
  95% of sequence in fragments shorter than    2205 bp

# BspKT6I.NruI (GATC+TCGCGA) double digest, 7213 thousand fragments (7199434+14872 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     448 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     633 bp
  50% of sequence in fragments shorter than     702 bp
  55% of sequence in fragments shorter than     778 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     950 bp
  70% of sequence in fragments shorter than    1048 bp
  75% of sequence in fragments shorter than    1164 bp
  80% of sequence in fragments shorter than    1307 bp
  85% of sequence in fragments shorter than    1488 bp
  90% of sequence in fragments shorter than    1746 bp
  95% of sequence in fragments shorter than    2208 bp

# BspKT6I.PvuI (GATC+CGATCG) double digest, 7213 thousand fragments (7199434+14186 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     390 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     569 bp
  45% of sequence in fragments shorter than     634 bp
  50% of sequence in fragments shorter than     703 bp
  55% of sequence in fragments shorter than     779 bp
  60% of sequence in fragments shorter than     862 bp
  65% of sequence in fragments shorter than     952 bp
  70% of sequence in fragments shorter than    1050 bp
  75% of sequence in fragments shorter than    1167 bp
  80% of sequence in fragments shorter than    1309 bp
  85% of sequence in fragments shorter than    1491 bp
  90% of sequence in fragments shorter than    1749 bp
  95% of sequence in fragments shorter than    2213 bp

# BspKT6I.FseI (GATC+GGCCGGCC) double digest, 7212 thousand fragments (7199434+13662 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     330 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     448 bp
  35% of sequence in fragments shorter than     504 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     633 bp
  50% of sequence in fragments shorter than     702 bp
  55% of sequence in fragments shorter than     777 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     950 bp
  70% of sequence in fragments shorter than    1047 bp
  75% of sequence in fragments shorter than    1164 bp
  80% of sequence in fragments shorter than    1306 bp
  85% of sequence in fragments shorter than    1487 bp
  90% of sequence in fragments shorter than    1744 bp
  95% of sequence in fragments shorter than    2206 bp

# BspKT6I.NotI (GATC+GCGGCCGC) double digest, 7208 thousand fragments (7199434+9795 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     448 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     633 bp
  50% of sequence in fragments shorter than     702 bp
  55% of sequence in fragments shorter than     777 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     950 bp
  70% of sequence in fragments shorter than    1048 bp
  75% of sequence in fragments shorter than    1164 bp
  80% of sequence in fragments shorter than    1306 bp
  85% of sequence in fragments shorter than    1488 bp
  90% of sequence in fragments shorter than    1745 bp
  95% of sequence in fragments shorter than    2208 bp

# BspKT6I.RsrII (GATC+CGGWCCG) double digest, 7208 thousand fragments (7199434+9764 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     448 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     633 bp
  50% of sequence in fragments shorter than     702 bp
  55% of sequence in fragments shorter than     778 bp
  60% of sequence in fragments shorter than     860 bp
  65% of sequence in fragments shorter than     950 bp
  70% of sequence in fragments shorter than    1048 bp
  75% of sequence in fragments shorter than    1165 bp
  80% of sequence in fragments shorter than    1307 bp
  85% of sequence in fragments shorter than    1488 bp
  90% of sequence in fragments shorter than    1746 bp
  95% of sequence in fragments shorter than    2208 bp

# AscI.BspKT6I (GGCGCGCC+GATC) double digest, 7203 thousand fragments (4751+7199434 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     198 bp
  15% of sequence in fragments shorter than     270 bp
  20% of sequence in fragments shorter than     331 bp
  25% of sequence in fragments shorter than     389 bp
  30% of sequence in fragments shorter than     449 bp
  35% of sequence in fragments shorter than     505 bp
  40% of sequence in fragments shorter than     568 bp
  45% of sequence in fragments shorter than     634 bp
  50% of sequence in fragments shorter than     703 bp
  55% of sequence in fragments shorter than     778 bp
  60% of sequence in fragments shorter than     861 bp
  65% of sequence in fragments shorter than     951 bp
  70% of sequence in fragments shorter than    1049 bp
  75% of sequence in fragments shorter than    1166 bp
  80% of sequence in fragments shorter than    1308 bp
  85% of sequence in fragments shorter than    1490 bp
  90% of sequence in fragments shorter than    1748 bp
  95% of sequence in fragments shorter than    2210 bp

# BsmAI.HgaI (GTCTC+GACGC) double digest, 7202 thousand fragments (6631585+571889 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     200 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     351 bp
  25% of sequence in fragments shorter than     422 bp
  30% of sequence in fragments shorter than     495 bp
  35% of sequence in fragments shorter than     567 bp
  40% of sequence in fragments shorter than     642 bp
  45% of sequence in fragments shorter than     724 bp
  50% of sequence in fragments shorter than     814 bp
  55% of sequence in fragments shorter than     910 bp
  60% of sequence in fragments shorter than    1018 bp
  65% of sequence in fragments shorter than    1136 bp
  70% of sequence in fragments shorter than    1276 bp
  75% of sequence in fragments shorter than    1439 bp
  80% of sequence in fragments shorter than    1635 bp
  85% of sequence in fragments shorter than    1899 bp
  90% of sequence in fragments shorter than    2262 bp
  95% of sequence in fragments shorter than    2920 bp

# BsmAI.EciI (GTCTC+GGCGGA) double digest, 7202 thousand fragments (6631585+571273 sites), 413 bp average fragment length
   5% of sequence in fragments shorter than     135 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     283 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     436 bp
  30% of sequence in fragments shorter than     512 bp
  35% of sequence in fragments shorter than     585 bp
  40% of sequence in fragments shorter than     664 bp
  45% of sequence in fragments shorter than     751 bp
  50% of sequence in fragments shorter than     844 bp
  55% of sequence in fragments shorter than     944 bp
  60% of sequence in fragments shorter than    1054 bp
  65% of sequence in fragments shorter than    1179 bp
  70% of sequence in fragments shorter than    1323 bp
  75% of sequence in fragments shorter than    1494 bp
  80% of sequence in fragments shorter than    1698 bp
  85% of sequence in fragments shorter than    1968 bp
  90% of sequence in fragments shorter than    2344 bp
  95% of sequence in fragments shorter than    3037 bp

# BsmAI.MfeI (GTCTC+CAATTG) double digest, 7195 thousand fragments (6631585+564303 sites), 414 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     348 bp
  25% of sequence in fragments shorter than     417 bp
  30% of sequence in fragments shorter than     487 bp
  35% of sequence in fragments shorter than     555 bp
  40% of sequence in fragments shorter than     625 bp
  45% of sequence in fragments shorter than     701 bp
  50% of sequence in fragments shorter than     783 bp
  55% of sequence in fragments shorter than     873 bp
  60% of sequence in fragments shorter than     970 bp
  65% of sequence in fragments shorter than    1078 bp
  70% of sequence in fragments shorter than    1203 bp
  75% of sequence in fragments shorter than    1351 bp
  80% of sequence in fragments shorter than    1527 bp
  85% of sequence in fragments shorter than    1757 bp
  90% of sequence in fragments shorter than    2073 bp
  95% of sequence in fragments shorter than    2633 bp

# Hin4II.SnaBI (CCTTC+TACGTA) double digest, 7191 thousand fragments (7059915+132299 sites), 414 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     425 bp
  30% of sequence in fragments shorter than     495 bp
  35% of sequence in fragments shorter than     560 bp
  40% of sequence in fragments shorter than     634 bp
  45% of sequence in fragments shorter than     709 bp
  50% of sequence in fragments shorter than     791 bp
  55% of sequence in fragments shorter than     878 bp
  60% of sequence in fragments shorter than     976 bp
  65% of sequence in fragments shorter than    1078 bp
  70% of sequence in fragments shorter than    1202 bp
  75% of sequence in fragments shorter than    1345 bp
  80% of sequence in fragments shorter than    1517 bp
  85% of sequence in fragments shorter than    1736 bp
  90% of sequence in fragments shorter than    2042 bp
  95% of sequence in fragments shorter than    2564 bp

# BspHI.BthCI (TCATGA+GCNGC) double digest, 7187 thousand fragments (978290+6209929 sites), 414 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     307 bp
  20% of sequence in fragments shorter than     385 bp
  25% of sequence in fragments shorter than     465 bp
  30% of sequence in fragments shorter than     545 bp
  35% of sequence in fragments shorter than     624 bp
  40% of sequence in fragments shorter than     710 bp
  45% of sequence in fragments shorter than     802 bp
  50% of sequence in fragments shorter than     894 bp
  55% of sequence in fragments shorter than    1001 bp
  60% of sequence in fragments shorter than    1113 bp
  65% of sequence in fragments shorter than    1242 bp
  70% of sequence in fragments shorter than    1385 bp
  75% of sequence in fragments shorter than    1560 bp
  80% of sequence in fragments shorter than    1765 bp
  85% of sequence in fragments shorter than    2031 bp
  90% of sequence in fragments shorter than    2390 bp
  95% of sequence in fragments shorter than    3007 bp

# Hin4II.NaeI (CCTTC+GCCGGC) double digest, 7185 thousand fragments (7059915+125692 sites), 414 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     426 bp
  30% of sequence in fragments shorter than     497 bp
  35% of sequence in fragments shorter than     563 bp
  40% of sequence in fragments shorter than     639 bp
  45% of sequence in fragments shorter than     716 bp
  50% of sequence in fragments shorter than     800 bp
  55% of sequence in fragments shorter than     890 bp
  60% of sequence in fragments shorter than     990 bp
  65% of sequence in fragments shorter than    1096 bp
  70% of sequence in fragments shorter than    1222 bp
  75% of sequence in fragments shorter than    1370 bp
  80% of sequence in fragments shorter than    1546 bp
  85% of sequence in fragments shorter than    1771 bp
  90% of sequence in fragments shorter than    2083 bp
  95% of sequence in fragments shorter than    2621 bp

# BscAI.SfeI (GCATC+CTRYAG) double digest, 7183 thousand fragments (3652926+3531009 sites), 414 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     284 bp
  20% of sequence in fragments shorter than     344 bp
  25% of sequence in fragments shorter than     404 bp
  30% of sequence in fragments shorter than     464 bp
  35% of sequence in fragments shorter than     524 bp
  40% of sequence in fragments shorter than     587 bp
  45% of sequence in fragments shorter than     651 bp
  50% of sequence in fragments shorter than     718 bp
  55% of sequence in fragments shorter than     794 bp
  60% of sequence in fragments shorter than     877 bp
  65% of sequence in fragments shorter than     969 bp
  70% of sequence in fragments shorter than    1069 bp
  75% of sequence in fragments shorter than    1189 bp
  80% of sequence in fragments shorter than    1332 bp
  85% of sequence in fragments shorter than    1513 bp
  90% of sequence in fragments shorter than    1764 bp
  95% of sequence in fragments shorter than    2197 bp

# BsmAI.SphI (GTCTC+GCATGC) double digest, 7182 thousand fragments (6631585+550952 sites), 414 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     352 bp
  25% of sequence in fragments shorter than     423 bp
  30% of sequence in fragments shorter than     495 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     638 bp
  45% of sequence in fragments shorter than     718 bp
  50% of sequence in fragments shorter than     805 bp
  55% of sequence in fragments shorter than     899 bp
  60% of sequence in fragments shorter than    1003 bp
  65% of sequence in fragments shorter than    1118 bp
  70% of sequence in fragments shorter than    1251 bp
  75% of sequence in fragments shorter than    1410 bp
  80% of sequence in fragments shorter than    1601 bp
  85% of sequence in fragments shorter than    1856 bp
  90% of sequence in fragments shorter than    2207 bp
  95% of sequence in fragments shorter than    2858 bp

# Hin4II.XhoI (CCTTC+CTCGAG) double digest, 7180 thousand fragments (7059915+121308 sites), 415 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     296 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     426 bp
  30% of sequence in fragments shorter than     496 bp
  35% of sequence in fragments shorter than     562 bp
  40% of sequence in fragments shorter than     637 bp
  45% of sequence in fragments shorter than     714 bp
  50% of sequence in fragments shorter than     798 bp
  55% of sequence in fragments shorter than     886 bp
  60% of sequence in fragments shorter than     986 bp
  65% of sequence in fragments shorter than    1090 bp
  70% of sequence in fragments shorter than    1215 bp
  75% of sequence in fragments shorter than    1362 bp
  80% of sequence in fragments shorter than    1536 bp
  85% of sequence in fragments shorter than    1758 bp
  90% of sequence in fragments shorter than    2069 bp
  95% of sequence in fragments shorter than    2601 bp

# BsrI.FauI (ACTGG+CCCGC) double digest, 7180 thousand fragments (5741310+1439772 sites), 415 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     284 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     414 bp
  30% of sequence in fragments shorter than     478 bp
  35% of sequence in fragments shorter than     545 bp
  40% of sequence in fragments shorter than     615 bp
  45% of sequence in fragments shorter than     687 bp
  50% of sequence in fragments shorter than     757 bp
  55% of sequence in fragments shorter than     841 bp
  60% of sequence in fragments shorter than     930 bp
  65% of sequence in fragments shorter than    1033 bp
  70% of sequence in fragments shorter than    1148 bp
  75% of sequence in fragments shorter than    1283 bp
  80% of sequence in fragments shorter than    1444 bp
  85% of sequence in fragments shorter than    1655 bp
  90% of sequence in fragments shorter than    1950 bp
  95% of sequence in fragments shorter than    2479 bp

# BspMI.BsrI (ACCTGC+ACTGG) double digest, 7178 thousand fragments (1438129+5741310 sites), 415 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     281 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     401 bp
  30% of sequence in fragments shorter than     462 bp
  35% of sequence in fragments shorter than     526 bp
  40% of sequence in fragments shorter than     593 bp
  45% of sequence in fragments shorter than     663 bp
  50% of sequence in fragments shorter than     729 bp
  55% of sequence in fragments shorter than     809 bp
  60% of sequence in fragments shorter than     889 bp
  65% of sequence in fragments shorter than     986 bp
  70% of sequence in fragments shorter than    1092 bp
  75% of sequence in fragments shorter than    1217 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1558 bp
  90% of sequence in fragments shorter than    1827 bp
  95% of sequence in fragments shorter than    2291 bp

# AvaI.BccI (CYCGRG+CCATC) double digest, 7176 thousand fragments (1005714+6170927 sites), 415 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     283 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     412 bp
  30% of sequence in fragments shorter than     476 bp
  35% of sequence in fragments shorter than     546 bp
  40% of sequence in fragments shorter than     618 bp
  45% of sequence in fragments shorter than     691 bp
  50% of sequence in fragments shorter than     773 bp
  55% of sequence in fragments shorter than     861 bp
  60% of sequence in fragments shorter than     958 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1186 bp
  75% of sequence in fragments shorter than    1331 bp
  80% of sequence in fragments shorter than    1514 bp
  85% of sequence in fragments shorter than    1747 bp
  90% of sequence in fragments shorter than    2095 bp
  95% of sequence in fragments shorter than    2736 bp

# BsmAI.ScaI (GTCTC+AGTACT) double digest, 7174 thousand fragments (6631585+543793 sites), 415 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     277 bp
  20% of sequence in fragments shorter than     349 bp
  25% of sequence in fragments shorter than     418 bp
  30% of sequence in fragments shorter than     487 bp
  35% of sequence in fragments shorter than     556 bp
  40% of sequence in fragments shorter than     626 bp
  45% of sequence in fragments shorter than     704 bp
  50% of sequence in fragments shorter than     787 bp
  55% of sequence in fragments shorter than     878 bp
  60% of sequence in fragments shorter than     977 bp
  65% of sequence in fragments shorter than    1088 bp
  70% of sequence in fragments shorter than    1214 bp
  75% of sequence in fragments shorter than    1366 bp
  80% of sequence in fragments shorter than    1549 bp
  85% of sequence in fragments shorter than    1785 bp
  90% of sequence in fragments shorter than    2122 bp
  95% of sequence in fragments shorter than    2732 bp

# ApoI.BglII (RAATTY+AGATCT) double digest, 7156 thousand fragments (6382433+774732 sites), 416 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     276 bp
  20% of sequence in fragments shorter than     341 bp
  25% of sequence in fragments shorter than     404 bp
  30% of sequence in fragments shorter than     471 bp
  35% of sequence in fragments shorter than     540 bp
  40% of sequence in fragments shorter than     611 bp
  45% of sequence in fragments shorter than     687 bp
  50% of sequence in fragments shorter than     766 bp
  55% of sequence in fragments shorter than     857 bp
  60% of sequence in fragments shorter than     954 bp
  65% of sequence in fragments shorter than    1065 bp
  70% of sequence in fragments shorter than    1194 bp
  75% of sequence in fragments shorter than    1341 bp
  80% of sequence in fragments shorter than    1531 bp
  85% of sequence in fragments shorter than    1792 bp
  90% of sequence in fragments shorter than    2187 bp
  95% of sequence in fragments shorter than    2990 bp

# BccI.BspHI (CCATC+TCATGA) double digest, 7148 thousand fragments (6170927+978290 sites), 416 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     409 bp
  30% of sequence in fragments shorter than     471 bp
  35% of sequence in fragments shorter than     535 bp
  40% of sequence in fragments shorter than     603 bp
  45% of sequence in fragments shorter than     673 bp
  50% of sequence in fragments shorter than     747 bp
  55% of sequence in fragments shorter than     828 bp
  60% of sequence in fragments shorter than     916 bp
  65% of sequence in fragments shorter than    1014 bp
  70% of sequence in fragments shorter than    1119 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1405 bp
  85% of sequence in fragments shorter than    1608 bp
  90% of sequence in fragments shorter than    1902 bp
  95% of sequence in fragments shorter than    2449 bp

# ClaI.Hin4II (ATCGAT+CCTTC) double digest, 7145 thousand fragments (86477+7059915 sites), 417 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     297 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     428 bp
  30% of sequence in fragments shorter than     498 bp
  35% of sequence in fragments shorter than     564 bp
  40% of sequence in fragments shorter than     640 bp
  45% of sequence in fragments shorter than     716 bp
  50% of sequence in fragments shorter than     800 bp
  55% of sequence in fragments shorter than     889 bp
  60% of sequence in fragments shorter than     989 bp
  65% of sequence in fragments shorter than    1093 bp
  70% of sequence in fragments shorter than    1218 bp
  75% of sequence in fragments shorter than    1365 bp
  80% of sequence in fragments shorter than    1538 bp
  85% of sequence in fragments shorter than    1760 bp
  90% of sequence in fragments shorter than    2070 bp
  95% of sequence in fragments shorter than    2602 bp

# AvaIII.BthCI (ATGCAT+GCNGC) double digest, 7142 thousand fragments (933357+6209929 sites), 417 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     310 bp
  20% of sequence in fragments shorter than     389 bp
  25% of sequence in fragments shorter than     469 bp
  30% of sequence in fragments shorter than     550 bp
  35% of sequence in fragments shorter than     635 bp
  40% of sequence in fragments shorter than     722 bp
  45% of sequence in fragments shorter than     816 bp
  50% of sequence in fragments shorter than     909 bp
  55% of sequence in fragments shorter than    1016 bp
  60% of sequence in fragments shorter than    1129 bp
  65% of sequence in fragments shorter than    1260 bp
  70% of sequence in fragments shorter than    1405 bp
  75% of sequence in fragments shorter than    1580 bp
  80% of sequence in fragments shorter than    1779 bp
  85% of sequence in fragments shorter than    2045 bp
  90% of sequence in fragments shorter than    2402 bp
  95% of sequence in fragments shorter than    3021 bp

# ApoI.NcoI (RAATTY+CCATGG) double digest, 7141 thousand fragments (6382433+759594 sites), 417 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     280 bp
  20% of sequence in fragments shorter than     343 bp
  25% of sequence in fragments shorter than     405 bp
  30% of sequence in fragments shorter than     470 bp
  35% of sequence in fragments shorter than     533 bp
  40% of sequence in fragments shorter than     602 bp
  45% of sequence in fragments shorter than     675 bp
  50% of sequence in fragments shorter than     751 bp
  55% of sequence in fragments shorter than     836 bp
  60% of sequence in fragments shorter than     926 bp
  65% of sequence in fragments shorter than    1032 bp
  70% of sequence in fragments shorter than    1153 bp
  75% of sequence in fragments shorter than    1295 bp
  80% of sequence in fragments shorter than    1452 bp
  85% of sequence in fragments shorter than    1682 bp
  90% of sequence in fragments shorter than    2010 bp
  95% of sequence in fragments shorter than    2618 bp

# StyI.Tsp45I (CCWWGG+GTSAC) double digest, 7135 thousand fragments (3114107+4021761 sites), 417 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     283 bp
  20% of sequence in fragments shorter than     344 bp
  25% of sequence in fragments shorter than     405 bp
  30% of sequence in fragments shorter than     468 bp
  35% of sequence in fragments shorter than     531 bp
  40% of sequence in fragments shorter than     597 bp
  45% of sequence in fragments shorter than     667 bp
  50% of sequence in fragments shorter than     741 bp
  55% of sequence in fragments shorter than     818 bp
  60% of sequence in fragments shorter than     907 bp
  65% of sequence in fragments shorter than    1006 bp
  70% of sequence in fragments shorter than    1120 bp
  75% of sequence in fragments shorter than    1253 bp
  80% of sequence in fragments shorter than    1416 bp
  85% of sequence in fragments shorter than    1632 bp
  90% of sequence in fragments shorter than    1934 bp
  95% of sequence in fragments shorter than    2464 bp

# BthCI.StuI (GCNGC+AGGCCT) double digest, 7135 thousand fragments (6209929+925729 sites), 417 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     388 bp
  25% of sequence in fragments shorter than     477 bp
  30% of sequence in fragments shorter than     563 bp
  35% of sequence in fragments shorter than     655 bp
  40% of sequence in fragments shorter than     749 bp
  45% of sequence in fragments shorter than     852 bp
  50% of sequence in fragments shorter than     958 bp
  55% of sequence in fragments shorter than    1079 bp
  60% of sequence in fragments shorter than    1212 bp
  65% of sequence in fragments shorter than    1362 bp
  70% of sequence in fragments shorter than    1529 bp
  75% of sequence in fragments shorter than    1729 bp
  80% of sequence in fragments shorter than    1955 bp
  85% of sequence in fragments shorter than    2260 bp
  90% of sequence in fragments shorter than    2693 bp
  95% of sequence in fragments shorter than    3426 bp

# AatII.Hin4II (GACGTC+CCTTC) double digest, 7130 thousand fragments (71089+7059915 sites), 417 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     363 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     642 bp
  45% of sequence in fragments shorter than     720 bp
  50% of sequence in fragments shorter than     804 bp
  55% of sequence in fragments shorter than     893 bp
  60% of sequence in fragments shorter than     994 bp
  65% of sequence in fragments shorter than    1099 bp
  70% of sequence in fragments shorter than    1225 bp
  75% of sequence in fragments shorter than    1373 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1773 bp
  90% of sequence in fragments shorter than    2085 bp
  95% of sequence in fragments shorter than    2623 bp

# ApaLI.BsmAI (GTGCAC+GTCTC) double digest, 7127 thousand fragments (496255+6631585 sites), 418 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     202 bp
  15% of sequence in fragments shorter than     280 bp
  20% of sequence in fragments shorter than     354 bp
  25% of sequence in fragments shorter than     425 bp
  30% of sequence in fragments shorter than     497 bp
  35% of sequence in fragments shorter than     568 bp
  40% of sequence in fragments shorter than     641 bp
  45% of sequence in fragments shorter than     722 bp
  50% of sequence in fragments shorter than     809 bp
  55% of sequence in fragments shorter than     903 bp
  60% of sequence in fragments shorter than    1007 bp
  65% of sequence in fragments shorter than    1122 bp
  70% of sequence in fragments shorter than    1256 bp
  75% of sequence in fragments shorter than    1413 bp
  80% of sequence in fragments shorter than    1601 bp
  85% of sequence in fragments shorter than    1842 bp
  90% of sequence in fragments shorter than    2169 bp
  95% of sequence in fragments shorter than    2808 bp

# Hin4II.SacII (CCTTC+CCGCGG) double digest, 7126 thousand fragments (7059915+66901 sites), 418 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     363 bp
  25% of sequence in fragments shorter than     430 bp
  30% of sequence in fragments shorter than     501 bp
  35% of sequence in fragments shorter than     568 bp
  40% of sequence in fragments shorter than     643 bp
  45% of sequence in fragments shorter than     722 bp
  50% of sequence in fragments shorter than     807 bp
  55% of sequence in fragments shorter than     897 bp
  60% of sequence in fragments shorter than     999 bp
  65% of sequence in fragments shorter than    1105 bp
  70% of sequence in fragments shorter than    1232 bp
  75% of sequence in fragments shorter than    1380 bp
  80% of sequence in fragments shorter than    1557 bp
  85% of sequence in fragments shorter than    1784 bp
  90% of sequence in fragments shorter than    2098 bp
  95% of sequence in fragments shorter than    2642 bp

# NspI.Tsp45I (RCATGY+GTSAC) double digest, 7125 thousand fragments (3104359+4021761 sites), 418 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     222 bp
  15% of sequence in fragments shorter than     288 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     467 bp
  35% of sequence in fragments shorter than     530 bp
  40% of sequence in fragments shorter than     594 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     732 bp
  55% of sequence in fragments shorter than     810 bp
  60% of sequence in fragments shorter than     893 bp
  65% of sequence in fragments shorter than     987 bp
  70% of sequence in fragments shorter than    1093 bp
  75% of sequence in fragments shorter than    1218 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1572 bp
  90% of sequence in fragments shorter than    1860 bp
  95% of sequence in fragments shorter than    2411 bp

# ApoI.BclI (RAATTY+TGATCA) double digest, 7120 thousand fragments (6382433+738785 sites), 418 bp average fragment length
   5% of sequence in fragments shorter than     136 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     278 bp
  20% of sequence in fragments shorter than     343 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     475 bp
  35% of sequence in fragments shorter than     546 bp
  40% of sequence in fragments shorter than     618 bp
  45% of sequence in fragments shorter than     694 bp
  50% of sequence in fragments shorter than     777 bp
  55% of sequence in fragments shorter than     866 bp
  60% of sequence in fragments shorter than     963 bp
  65% of sequence in fragments shorter than    1076 bp
  70% of sequence in fragments shorter than    1208 bp
  75% of sequence in fragments shorter than    1356 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1813 bp
  90% of sequence in fragments shorter than    2208 bp
  95% of sequence in fragments shorter than    3018 bp

# Hin4II.PspXI (CCTTC+VCTCGAGB) double digest, 7118 thousand fragments (7059915+59193 sites), 418 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     430 bp
  30% of sequence in fragments shorter than     501 bp
  35% of sequence in fragments shorter than     567 bp
  40% of sequence in fragments shorter than     642 bp
  45% of sequence in fragments shorter than     721 bp
  50% of sequence in fragments shorter than     805 bp
  55% of sequence in fragments shorter than     895 bp
  60% of sequence in fragments shorter than     995 bp
  65% of sequence in fragments shorter than    1101 bp
  70% of sequence in fragments shorter than    1227 bp
  75% of sequence in fragments shorter than    1375 bp
  80% of sequence in fragments shorter than    1550 bp
  85% of sequence in fragments shorter than    1776 bp
  90% of sequence in fragments shorter than    2088 bp
  95% of sequence in fragments shorter than    2628 bp

# ApoI.FnuDII (RAATTY+CGCG) double digest, 7115 thousand fragments (6382433+733940 sites), 418 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     283 bp
  20% of sequence in fragments shorter than     348 bp
  25% of sequence in fragments shorter than     414 bp
  30% of sequence in fragments shorter than     482 bp
  35% of sequence in fragments shorter than     553 bp
  40% of sequence in fragments shorter than     624 bp
  45% of sequence in fragments shorter than     699 bp
  50% of sequence in fragments shorter than     782 bp
  55% of sequence in fragments shorter than     870 bp
  60% of sequence in fragments shorter than     963 bp
  65% of sequence in fragments shorter than    1070 bp
  70% of sequence in fragments shorter than    1194 bp
  75% of sequence in fragments shorter than    1332 bp
  80% of sequence in fragments shorter than    1505 bp
  85% of sequence in fragments shorter than    1736 bp
  90% of sequence in fragments shorter than    2072 bp
  95% of sequence in fragments shorter than    2681 bp

# BthCI.NdeI (GCNGC+CATATG) double digest, 7112 thousand fragments (6209929+903360 sites), 418 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     312 bp
  20% of sequence in fragments shorter than     392 bp
  25% of sequence in fragments shorter than     471 bp
  30% of sequence in fragments shorter than     555 bp
  35% of sequence in fragments shorter than     641 bp
  40% of sequence in fragments shorter than     730 bp
  45% of sequence in fragments shorter than     823 bp
  50% of sequence in fragments shorter than     912 bp
  55% of sequence in fragments shorter than    1020 bp
  60% of sequence in fragments shorter than    1134 bp
  65% of sequence in fragments shorter than    1261 bp
  70% of sequence in fragments shorter than    1407 bp
  75% of sequence in fragments shorter than    1581 bp
  80% of sequence in fragments shorter than    1787 bp
  85% of sequence in fragments shorter than    2054 bp
  90% of sequence in fragments shorter than    2411 bp
  95% of sequence in fragments shorter than    3018 bp

# AgeI.Hin4II (ACCGGT+CCTTC) double digest, 7112 thousand fragments (53386+7059915 sites), 418 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     431 bp
  30% of sequence in fragments shorter than     502 bp
  35% of sequence in fragments shorter than     568 bp
  40% of sequence in fragments shorter than     642 bp
  45% of sequence in fragments shorter than     721 bp
  50% of sequence in fragments shorter than     806 bp
  55% of sequence in fragments shorter than     895 bp
  60% of sequence in fragments shorter than     996 bp
  65% of sequence in fragments shorter than    1101 bp
  70% of sequence in fragments shorter than    1227 bp
  75% of sequence in fragments shorter than    1376 bp
  80% of sequence in fragments shorter than    1551 bp
  85% of sequence in fragments shorter than    1776 bp
  90% of sequence in fragments shorter than    2089 bp
  95% of sequence in fragments shorter than    2628 bp

# AccI.HphI (GTMKAC+GGTGA) double digest, 7107 thousand fragments (1101063+6007336 sites), 419 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     215 bp
  15% of sequence in fragments shorter than     281 bp
  20% of sequence in fragments shorter than     342 bp
  25% of sequence in fragments shorter than     401 bp
  30% of sequence in fragments shorter than     461 bp
  35% of sequence in fragments shorter than     521 bp
  40% of sequence in fragments shorter than     584 bp
  45% of sequence in fragments shorter than     648 bp
  50% of sequence in fragments shorter than     716 bp
  55% of sequence in fragments shorter than     792 bp
  60% of sequence in fragments shorter than     870 bp
  65% of sequence in fragments shorter than     963 bp
  70% of sequence in fragments shorter than    1068 bp
  75% of sequence in fragments shorter than    1191 bp
  80% of sequence in fragments shorter than    1335 bp
  85% of sequence in fragments shorter than    1506 bp
  90% of sequence in fragments shorter than    1771 bp
  95% of sequence in fragments shorter than    2199 bp

# AvaIII.BccI (ATGCAT+CCATC) double digest, 7103 thousand fragments (933357+6170927 sites), 419 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     468 bp
  35% of sequence in fragments shorter than     531 bp
  40% of sequence in fragments shorter than     599 bp
  45% of sequence in fragments shorter than     666 bp
  50% of sequence in fragments shorter than     739 bp
  55% of sequence in fragments shorter than     817 bp
  60% of sequence in fragments shorter than     903 bp
  65% of sequence in fragments shorter than     999 bp
  70% of sequence in fragments shorter than    1102 bp
  75% of sequence in fragments shorter than    1228 bp
  80% of sequence in fragments shorter than    1381 bp
  85% of sequence in fragments shorter than    1581 bp
  90% of sequence in fragments shorter than    1864 bp
  95% of sequence in fragments shorter than    2389 bp

# Hin4II.PmeI (CCTTC+GTTTAAAC) double digest, 7101 thousand fragments (7059915+42094 sites), 419 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     431 bp
  30% of sequence in fragments shorter than     502 bp
  35% of sequence in fragments shorter than     568 bp
  40% of sequence in fragments shorter than     642 bp
  45% of sequence in fragments shorter than     722 bp
  50% of sequence in fragments shorter than     806 bp
  55% of sequence in fragments shorter than     895 bp
  60% of sequence in fragments shorter than     995 bp
  65% of sequence in fragments shorter than    1101 bp
  70% of sequence in fragments shorter than    1226 bp
  75% of sequence in fragments shorter than    1374 bp
  80% of sequence in fragments shorter than    1549 bp
  85% of sequence in fragments shorter than    1774 bp
  90% of sequence in fragments shorter than    2085 bp
  95% of sequence in fragments shorter than    2623 bp

# BccI.StuI (CCATC+AGGCCT) double digest, 7096 thousand fragments (6170927+925729 sites), 419 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     218 bp
  15% of sequence in fragments shorter than     286 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     471 bp
  35% of sequence in fragments shorter than     536 bp
  40% of sequence in fragments shorter than     607 bp
  45% of sequence in fragments shorter than     679 bp
  50% of sequence in fragments shorter than     752 bp
  55% of sequence in fragments shorter than     837 bp
  60% of sequence in fragments shorter than     928 bp
  65% of sequence in fragments shorter than    1032 bp
  70% of sequence in fragments shorter than    1141 bp
  75% of sequence in fragments shorter than    1276 bp
  80% of sequence in fragments shorter than    1435 bp
  85% of sequence in fragments shorter than    1644 bp
  90% of sequence in fragments shorter than    1937 bp
  95% of sequence in fragments shorter than    2442 bp

# ApoI.BsaAI (RAATTY+YACGTR) double digest, 7095 thousand fragments (6382433+713801 sites), 419 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     281 bp
  20% of sequence in fragments shorter than     345 bp
  25% of sequence in fragments shorter than     409 bp
  30% of sequence in fragments shorter than     475 bp
  35% of sequence in fragments shorter than     544 bp
  40% of sequence in fragments shorter than     615 bp
  45% of sequence in fragments shorter than     690 bp
  50% of sequence in fragments shorter than     769 bp
  55% of sequence in fragments shorter than     856 bp
  60% of sequence in fragments shorter than     951 bp
  65% of sequence in fragments shorter than    1061 bp
  70% of sequence in fragments shorter than    1185 bp
  75% of sequence in fragments shorter than    1324 bp
  80% of sequence in fragments shorter than    1499 bp
  85% of sequence in fragments shorter than    1736 bp
  90% of sequence in fragments shorter than    2086 bp
  95% of sequence in fragments shorter than    2743 bp

# HphI.PpuMI (GGTGA+RGGWCCY) double digest, 7091 thousand fragments (6007336+1085138 sites), 420 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     281 bp
  20% of sequence in fragments shorter than     343 bp
  25% of sequence in fragments shorter than     403 bp
  30% of sequence in fragments shorter than     467 bp
  35% of sequence in fragments shorter than     531 bp
  40% of sequence in fragments shorter than     598 bp
  45% of sequence in fragments shorter than     670 bp
  50% of sequence in fragments shorter than     745 bp
  55% of sequence in fragments shorter than     825 bp
  60% of sequence in fragments shorter than     914 bp
  65% of sequence in fragments shorter than    1016 bp
  70% of sequence in fragments shorter than    1131 bp
  75% of sequence in fragments shorter than    1266 bp
  80% of sequence in fragments shorter than    1420 bp
  85% of sequence in fragments shorter than    1632 bp
  90% of sequence in fragments shorter than    1936 bp
  95% of sequence in fragments shorter than    2454 bp

# ApaI.BsmAI (GGGCCC+GTCTC) double digest, 7091 thousand fragments (460339+6631585 sites), 420 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     201 bp
  15% of sequence in fragments shorter than     279 bp
  20% of sequence in fragments shorter than     352 bp
  25% of sequence in fragments shorter than     424 bp
  30% of sequence in fragments shorter than     497 bp
  35% of sequence in fragments shorter than     569 bp
  40% of sequence in fragments shorter than     644 bp
  45% of sequence in fragments shorter than     727 bp
  50% of sequence in fragments shorter than     818 bp
  55% of sequence in fragments shorter than     916 bp
  60% of sequence in fragments shorter than    1026 bp
  65% of sequence in fragments shorter than    1147 bp
  70% of sequence in fragments shorter than    1288 bp
  75% of sequence in fragments shorter than    1458 bp
  80% of sequence in fragments shorter than    1658 bp
  85% of sequence in fragments shorter than    1927 bp
  90% of sequence in fragments shorter than    2300 bp
  95% of sequence in fragments shorter than    3001 bp

# HphI.PvuII (GGTGA+CAGCTG) double digest, 7091 thousand fragments (6007336+1084260 sites), 420 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     281 bp
  20% of sequence in fragments shorter than     344 bp
  25% of sequence in fragments shorter than     405 bp
  30% of sequence in fragments shorter than     468 bp
  35% of sequence in fragments shorter than     532 bp
  40% of sequence in fragments shorter than     597 bp
  45% of sequence in fragments shorter than     668 bp
  50% of sequence in fragments shorter than     742 bp
  55% of sequence in fragments shorter than     821 bp
  60% of sequence in fragments shorter than     910 bp
  65% of sequence in fragments shorter than    1013 bp
  70% of sequence in fragments shorter than    1128 bp
  75% of sequence in fragments shorter than    1263 bp
  80% of sequence in fragments shorter than    1413 bp
  85% of sequence in fragments shorter than    1626 bp
  90% of sequence in fragments shorter than    1932 bp
  95% of sequence in fragments shorter than    2448 bp

# Hin4II.SalI (CCTTC+GTCGAC) double digest, 7090 thousand fragments (7059915+31396 sites), 420 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     432 bp
  30% of sequence in fragments shorter than     503 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     644 bp
  45% of sequence in fragments shorter than     724 bp
  50% of sequence in fragments shorter than     808 bp
  55% of sequence in fragments shorter than     898 bp
  60% of sequence in fragments shorter than     999 bp
  65% of sequence in fragments shorter than    1105 bp
  70% of sequence in fragments shorter than    1231 bp
  75% of sequence in fragments shorter than    1379 bp
  80% of sequence in fragments shorter than    1555 bp
  85% of sequence in fragments shorter than    1782 bp
  90% of sequence in fragments shorter than    2094 bp
  95% of sequence in fragments shorter than    2635 bp

# ApoI.BceAI (RAATTY+ACGGC) double digest, 7087 thousand fragments (6382433+705178 sites), 420 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     218 bp
  15% of sequence in fragments shorter than     282 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     409 bp
  30% of sequence in fragments shorter than     476 bp
  35% of sequence in fragments shorter than     544 bp
  40% of sequence in fragments shorter than     613 bp
  45% of sequence in fragments shorter than     686 bp
  50% of sequence in fragments shorter than     764 bp
  55% of sequence in fragments shorter than     849 bp
  60% of sequence in fragments shorter than     937 bp
  65% of sequence in fragments shorter than    1040 bp
  70% of sequence in fragments shorter than    1156 bp
  75% of sequence in fragments shorter than    1294 bp
  80% of sequence in fragments shorter than    1448 bp
  85% of sequence in fragments shorter than    1664 bp
  90% of sequence in fragments shorter than    1970 bp
  95% of sequence in fragments shorter than    2517 bp

# Hin4II.MluI (CCTTC+ACGCGT) double digest, 7080 thousand fragments (7059915+21415 sites), 420 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     645 bp
  45% of sequence in fragments shorter than     725 bp
  50% of sequence in fragments shorter than     809 bp
  55% of sequence in fragments shorter than     899 bp
  60% of sequence in fragments shorter than    1001 bp
  65% of sequence in fragments shorter than    1106 bp
  70% of sequence in fragments shorter than    1233 bp
  75% of sequence in fragments shorter than    1381 bp
  80% of sequence in fragments shorter than    1558 bp
  85% of sequence in fragments shorter than    1785 bp
  90% of sequence in fragments shorter than    2098 bp
  95% of sequence in fragments shorter than    2641 bp

# ApoI.BciVI (RAATTY+GTATCC) double digest, 7078 thousand fragments (6382433+696093 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     215 bp
  15% of sequence in fragments shorter than     280 bp
  20% of sequence in fragments shorter than     345 bp
  25% of sequence in fragments shorter than     408 bp
  30% of sequence in fragments shorter than     476 bp
  35% of sequence in fragments shorter than     546 bp
  40% of sequence in fragments shorter than     618 bp
  45% of sequence in fragments shorter than     694 bp
  50% of sequence in fragments shorter than     775 bp
  55% of sequence in fragments shorter than     863 bp
  60% of sequence in fragments shorter than     958 bp
  65% of sequence in fragments shorter than    1071 bp
  70% of sequence in fragments shorter than    1202 bp
  75% of sequence in fragments shorter than    1357 bp
  80% of sequence in fragments shorter than    1549 bp
  85% of sequence in fragments shorter than    1814 bp
  90% of sequence in fragments shorter than    2215 bp
  95% of sequence in fragments shorter than    3012 bp

# BsmAI.EcoRV (GTCTC+GATATC) double digest, 7077 thousand fragments (6631585+446473 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     202 bp
  15% of sequence in fragments shorter than     281 bp
  20% of sequence in fragments shorter than     355 bp
  25% of sequence in fragments shorter than     424 bp
  30% of sequence in fragments shorter than     495 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     638 bp
  45% of sequence in fragments shorter than     717 bp
  50% of sequence in fragments shorter than     802 bp
  55% of sequence in fragments shorter than     893 bp
  60% of sequence in fragments shorter than     993 bp
  65% of sequence in fragments shorter than    1106 bp
  70% of sequence in fragments shorter than    1234 bp
  75% of sequence in fragments shorter than    1385 bp
  80% of sequence in fragments shorter than    1569 bp
  85% of sequence in fragments shorter than    1809 bp
  90% of sequence in fragments shorter than    2141 bp
  95% of sequence in fragments shorter than    2745 bp

# Hin4II.SgrAI (CCTTC+CRCCGGYG) double digest, 7074 thousand fragments (7059915+15270 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     726 bp
  50% of sequence in fragments shorter than     811 bp
  55% of sequence in fragments shorter than     900 bp
  60% of sequence in fragments shorter than    1002 bp
  65% of sequence in fragments shorter than    1108 bp
  70% of sequence in fragments shorter than    1235 bp
  75% of sequence in fragments shorter than    1383 bp
  80% of sequence in fragments shorter than    1561 bp
  85% of sequence in fragments shorter than    1788 bp
  90% of sequence in fragments shorter than    2102 bp
  95% of sequence in fragments shorter than    2646 bp

# Hin4II.NruI (CCTTC+TCGCGA) double digest, 7074 thousand fragments (7059915+14872 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     725 bp
  50% of sequence in fragments shorter than     810 bp
  55% of sequence in fragments shorter than     900 bp
  60% of sequence in fragments shorter than    1001 bp
  65% of sequence in fragments shorter than    1107 bp
  70% of sequence in fragments shorter than    1234 bp
  75% of sequence in fragments shorter than    1382 bp
  80% of sequence in fragments shorter than    1559 bp
  85% of sequence in fragments shorter than    1786 bp
  90% of sequence in fragments shorter than    2100 bp
  95% of sequence in fragments shorter than    2642 bp

# BccI.NdeI (CCATC+CATATG) double digest, 7073 thousand fragments (6170927+903360 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     220 bp
  15% of sequence in fragments shorter than     287 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     410 bp
  30% of sequence in fragments shorter than     472 bp
  35% of sequence in fragments shorter than     536 bp
  40% of sequence in fragments shorter than     605 bp
  45% of sequence in fragments shorter than     674 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     829 bp
  60% of sequence in fragments shorter than     916 bp
  65% of sequence in fragments shorter than    1014 bp
  70% of sequence in fragments shorter than    1119 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1404 bp
  85% of sequence in fragments shorter than    1606 bp
  90% of sequence in fragments shorter than    1899 bp
  95% of sequence in fragments shorter than    2448 bp

# Hin4II.PvuI (CCTTC+CGATCG) double digest, 7073 thousand fragments (7059915+14186 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     725 bp
  50% of sequence in fragments shorter than     810 bp
  55% of sequence in fragments shorter than     900 bp
  60% of sequence in fragments shorter than    1001 bp
  65% of sequence in fragments shorter than    1107 bp
  70% of sequence in fragments shorter than    1234 bp
  75% of sequence in fragments shorter than    1382 bp
  80% of sequence in fragments shorter than    1559 bp
  85% of sequence in fragments shorter than    1786 bp
  90% of sequence in fragments shorter than    2100 bp
  95% of sequence in fragments shorter than    2643 bp

# FseI.Hin4II (GGCCGGCC+CCTTC) double digest, 7073 thousand fragments (13662+7059915 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     726 bp
  50% of sequence in fragments shorter than     811 bp
  55% of sequence in fragments shorter than     901 bp
  60% of sequence in fragments shorter than    1002 bp
  65% of sequence in fragments shorter than    1108 bp
  70% of sequence in fragments shorter than    1235 bp
  75% of sequence in fragments shorter than    1383 bp
  80% of sequence in fragments shorter than    1561 bp
  85% of sequence in fragments shorter than    1787 bp
  90% of sequence in fragments shorter than    2102 bp
  95% of sequence in fragments shorter than    2646 bp

# BthCI.HindIII (GCNGC+AAGCTT) double digest, 7069 thousand fragments (6209929+860365 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     314 bp
  20% of sequence in fragments shorter than     394 bp
  25% of sequence in fragments shorter than     477 bp
  30% of sequence in fragments shorter than     560 bp
  35% of sequence in fragments shorter than     646 bp
  40% of sequence in fragments shorter than     738 bp
  45% of sequence in fragments shorter than     833 bp
  50% of sequence in fragments shorter than     930 bp
  55% of sequence in fragments shorter than    1037 bp
  60% of sequence in fragments shorter than    1156 bp
  65% of sequence in fragments shorter than    1289 bp
  70% of sequence in fragments shorter than    1441 bp
  75% of sequence in fragments shorter than    1614 bp
  80% of sequence in fragments shorter than    1826 bp
  85% of sequence in fragments shorter than    2079 bp
  90% of sequence in fragments shorter than    2459 bp
  95% of sequence in fragments shorter than    3112 bp

# Hin4II.NotI (CCTTC+GCGGCCGC) double digest, 7069 thousand fragments (7059915+9795 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     505 bp
  35% of sequence in fragments shorter than     572 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     726 bp
  50% of sequence in fragments shorter than     811 bp
  55% of sequence in fragments shorter than     901 bp
  60% of sequence in fragments shorter than    1003 bp
  65% of sequence in fragments shorter than    1109 bp
  70% of sequence in fragments shorter than    1236 bp
  75% of sequence in fragments shorter than    1384 bp
  80% of sequence in fragments shorter than    1562 bp
  85% of sequence in fragments shorter than    1789 bp
  90% of sequence in fragments shorter than    2103 bp
  95% of sequence in fragments shorter than    2648 bp

# Hin4II.RsrII (CCTTC+CGGWCCG) double digest, 7069 thousand fragments (7059915+9764 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     505 bp
  35% of sequence in fragments shorter than     572 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     726 bp
  50% of sequence in fragments shorter than     811 bp
  55% of sequence in fragments shorter than     901 bp
  60% of sequence in fragments shorter than    1002 bp
  65% of sequence in fragments shorter than    1109 bp
  70% of sequence in fragments shorter than    1236 bp
  75% of sequence in fragments shorter than    1383 bp
  80% of sequence in fragments shorter than    1561 bp
  85% of sequence in fragments shorter than    1788 bp
  90% of sequence in fragments shorter than    2103 bp
  95% of sequence in fragments shorter than    2647 bp

# AscI.Hin4II (GGCGCGCC+CCTTC) double digest, 7064 thousand fragments (4751+7059915 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     434 bp
  30% of sequence in fragments shorter than     505 bp
  35% of sequence in fragments shorter than     572 bp
  40% of sequence in fragments shorter than     647 bp
  45% of sequence in fragments shorter than     727 bp
  50% of sequence in fragments shorter than     812 bp
  55% of sequence in fragments shorter than     901 bp
  60% of sequence in fragments shorter than    1003 bp
  65% of sequence in fragments shorter than    1109 bp
  70% of sequence in fragments shorter than    1236 bp
  75% of sequence in fragments shorter than    1384 bp
  80% of sequence in fragments shorter than    1562 bp
  85% of sequence in fragments shorter than    1789 bp
  90% of sequence in fragments shorter than    2104 bp
  95% of sequence in fragments shorter than    2648 bp

# BsrI.PstI (ACTGG+CTGCAG) double digest, 7062 thousand fragments (5741310+1321469 sites), 421 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     284 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     413 bp
  30% of sequence in fragments shorter than     475 bp
  35% of sequence in fragments shorter than     539 bp
  40% of sequence in fragments shorter than     608 bp
  45% of sequence in fragments shorter than     679 bp
  50% of sequence in fragments shorter than     747 bp
  55% of sequence in fragments shorter than     830 bp
  60% of sequence in fragments shorter than     917 bp
  65% of sequence in fragments shorter than    1017 bp
  70% of sequence in fragments shorter than    1129 bp
  75% of sequence in fragments shorter than    1260 bp
  80% of sequence in fragments shorter than    1411 bp
  85% of sequence in fragments shorter than    1611 bp
  90% of sequence in fragments shorter than    1898 bp
  95% of sequence in fragments shorter than    2404 bp

# BthCI.XbaI (GCNGC+TCTAGA) double digest, 7060 thousand fragments (6209929+850998 sites), 422 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     315 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     473 bp
  30% of sequence in fragments shorter than     558 bp
  35% of sequence in fragments shorter than     644 bp
  40% of sequence in fragments shorter than     735 bp
  45% of sequence in fragments shorter than     831 bp
  50% of sequence in fragments shorter than     929 bp
  55% of sequence in fragments shorter than    1039 bp
  60% of sequence in fragments shorter than    1161 bp
  65% of sequence in fragments shorter than    1296 bp
  70% of sequence in fragments shorter than    1446 bp
  75% of sequence in fragments shorter than    1623 bp
  80% of sequence in fragments shorter than    1829 bp
  85% of sequence in fragments shorter than    2097 bp
  90% of sequence in fragments shorter than    2482 bp
  95% of sequence in fragments shorter than    3134 bp

# BthCI.EcoRI (GCNGC+GAATTC) double digest, 7056 thousand fragments (6209929+847401 sites), 422 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     313 bp
  20% of sequence in fragments shorter than     392 bp
  25% of sequence in fragments shorter than     475 bp
  30% of sequence in fragments shorter than     560 bp
  35% of sequence in fragments shorter than     646 bp
  40% of sequence in fragments shorter than     735 bp
  45% of sequence in fragments shorter than     832 bp
  50% of sequence in fragments shorter than     929 bp
  55% of sequence in fragments shorter than    1038 bp
  60% of sequence in fragments shorter than    1158 bp
  65% of sequence in fragments shorter than    1291 bp
  70% of sequence in fragments shorter than    1443 bp
  75% of sequence in fragments shorter than    1618 bp
  80% of sequence in fragments shorter than    1826 bp
  85% of sequence in fragments shorter than    2082 bp
  90% of sequence in fragments shorter than    2457 bp
  95% of sequence in fragments shorter than    3095 bp

# BbvI.HhaI (GCAGC+GCGC) double digest, 7046 thousand fragments (5290051+1756502 sites), 422 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     392 bp
  25% of sequence in fragments shorter than     478 bp
  30% of sequence in fragments shorter than     566 bp
  35% of sequence in fragments shorter than     658 bp
  40% of sequence in fragments shorter than     750 bp
  45% of sequence in fragments shorter than     853 bp
  50% of sequence in fragments shorter than     958 bp
  55% of sequence in fragments shorter than    1078 bp
  60% of sequence in fragments shorter than    1212 bp
  65% of sequence in fragments shorter than    1362 bp
  70% of sequence in fragments shorter than    1534 bp
  75% of sequence in fragments shorter than    1723 bp
  80% of sequence in fragments shorter than    1960 bp
  85% of sequence in fragments shorter than    2262 bp
  90% of sequence in fragments shorter than    2706 bp
  95% of sequence in fragments shorter than    3465 bp

# MlyI.StyI (GAGTC+CCWWGG) double digest, 7045 thousand fragments (3931692+3114107 sites), 422 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     221 bp
  15% of sequence in fragments shorter than     282 bp
  20% of sequence in fragments shorter than     345 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     472 bp
  35% of sequence in fragments shorter than     534 bp
  40% of sequence in fragments shorter than     603 bp
  45% of sequence in fragments shorter than     674 bp
  50% of sequence in fragments shorter than     750 bp
  55% of sequence in fragments shorter than     828 bp
  60% of sequence in fragments shorter than     914 bp
  65% of sequence in fragments shorter than    1015 bp
  70% of sequence in fragments shorter than    1132 bp
  75% of sequence in fragments shorter than    1271 bp
  80% of sequence in fragments shorter than    1437 bp
  85% of sequence in fragments shorter than    1662 bp
  90% of sequence in fragments shorter than    1973 bp
  95% of sequence in fragments shorter than    2589 bp

# MlyI.NspI (GAGTC+RCATGY) double digest, 7035 thousand fragments (3931692+3104359 sites), 423 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     222 bp
  15% of sequence in fragments shorter than     287 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     404 bp
  30% of sequence in fragments shorter than     463 bp
  35% of sequence in fragments shorter than     524 bp
  40% of sequence in fragments shorter than     587 bp
  45% of sequence in fragments shorter than     653 bp
  50% of sequence in fragments shorter than     722 bp
  55% of sequence in fragments shorter than     798 bp
  60% of sequence in fragments shorter than     879 bp
  65% of sequence in fragments shorter than     971 bp
  70% of sequence in fragments shorter than    1073 bp
  75% of sequence in fragments shorter than    1196 bp
  80% of sequence in fragments shorter than    1347 bp
  85% of sequence in fragments shorter than    1540 bp
  90% of sequence in fragments shorter than    1819 bp
  95% of sequence in fragments shorter than    2307 bp

# BsmAI.SpeI (GTCTC+ACTAGT) double digest, 7031 thousand fragments (6631585+400286 sites), 423 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     501 bp
  35% of sequence in fragments shorter than     572 bp
  40% of sequence in fragments shorter than     644 bp
  45% of sequence in fragments shorter than     724 bp
  50% of sequence in fragments shorter than     809 bp
  55% of sequence in fragments shorter than     901 bp
  60% of sequence in fragments shorter than    1002 bp
  65% of sequence in fragments shorter than    1114 bp
  70% of sequence in fragments shorter than    1242 bp
  75% of sequence in fragments shorter than    1382 bp
  80% of sequence in fragments shorter than    1563 bp
  85% of sequence in fragments shorter than    1796 bp
  90% of sequence in fragments shorter than    2123 bp
  95% of sequence in fragments shorter than    2727 bp

# BccI.HindIII (CCATC+AAGCTT) double digest, 7030 thousand fragments (6170927+860365 sites), 423 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     220 bp
  15% of sequence in fragments shorter than     287 bp
  20% of sequence in fragments shorter than     348 bp
  25% of sequence in fragments shorter than     409 bp
  30% of sequence in fragments shorter than     471 bp
  35% of sequence in fragments shorter than     536 bp
  40% of sequence in fragments shorter than     604 bp
  45% of sequence in fragments shorter than     672 bp
  50% of sequence in fragments shorter than     747 bp
  55% of sequence in fragments shorter than     827 bp
  60% of sequence in fragments shorter than     914 bp
  65% of sequence in fragments shorter than    1012 bp
  70% of sequence in fragments shorter than    1118 bp
  75% of sequence in fragments shorter than    1245 bp
  80% of sequence in fragments shorter than    1401 bp
  85% of sequence in fragments shorter than    1604 bp
  90% of sequence in fragments shorter than    1894 bp
  95% of sequence in fragments shorter than    2431 bp

# AflII.ApoI (CTTAAG+RAATTY) double digest, 7025 thousand fragments (643284+6382433 sites), 424 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     217 bp
  15% of sequence in fragments shorter than     283 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     412 bp
  30% of sequence in fragments shorter than     482 bp
  35% of sequence in fragments shorter than     554 bp
  40% of sequence in fragments shorter than     627 bp
  45% of sequence in fragments shorter than     705 bp
  50% of sequence in fragments shorter than     792 bp
  55% of sequence in fragments shorter than     883 bp
  60% of sequence in fragments shorter than     977 bp
  65% of sequence in fragments shorter than    1092 bp
  70% of sequence in fragments shorter than    1227 bp
  75% of sequence in fragments shorter than    1384 bp
  80% of sequence in fragments shorter than    1586 bp
  85% of sequence in fragments shorter than    1863 bp
  90% of sequence in fragments shorter than    2277 bp
  95% of sequence in fragments shorter than    3148 bp

# BccI.XbaI (CCATC+TCTAGA) double digest, 7021 thousand fragments (6170927+850998 sites), 424 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     222 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     352 bp
  25% of sequence in fragments shorter than     414 bp
  30% of sequence in fragments shorter than     476 bp
  35% of sequence in fragments shorter than     542 bp
  40% of sequence in fragments shorter than     611 bp
  45% of sequence in fragments shorter than     682 bp
  50% of sequence in fragments shorter than     755 bp
  55% of sequence in fragments shorter than     837 bp
  60% of sequence in fragments shorter than     925 bp
  65% of sequence in fragments shorter than    1024 bp
  70% of sequence in fragments shorter than    1127 bp
  75% of sequence in fragments shorter than    1253 bp
  80% of sequence in fragments shorter than    1409 bp
  85% of sequence in fragments shorter than    1612 bp
  90% of sequence in fragments shorter than    1894 bp
  95% of sequence in fragments shorter than    2392 bp

# BsmAI.HpaI (GTCTC+GTTAAC) double digest, 7020 thousand fragments (6631585+389418 sites), 424 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     284 bp
  20% of sequence in fragments shorter than     358 bp
  25% of sequence in fragments shorter than     428 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     643 bp
  45% of sequence in fragments shorter than     724 bp
  50% of sequence in fragments shorter than     810 bp
  55% of sequence in fragments shorter than     904 bp
  60% of sequence in fragments shorter than    1007 bp
  65% of sequence in fragments shorter than    1121 bp
  70% of sequence in fragments shorter than    1253 bp
  75% of sequence in fragments shorter than    1411 bp
  80% of sequence in fragments shorter than    1599 bp
  85% of sequence in fragments shorter than    1850 bp
  90% of sequence in fragments shorter than    2193 bp
  95% of sequence in fragments shorter than    2829 bp

# BccI.EcoRI (CCATC+GAATTC) double digest, 7017 thousand fragments (6170927+847401 sites), 424 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     348 bp
  25% of sequence in fragments shorter than     411 bp
  30% of sequence in fragments shorter than     473 bp
  35% of sequence in fragments shorter than     539 bp
  40% of sequence in fragments shorter than     607 bp
  45% of sequence in fragments shorter than     677 bp
  50% of sequence in fragments shorter than     748 bp
  55% of sequence in fragments shorter than     828 bp
  60% of sequence in fragments shorter than     915 bp
  65% of sequence in fragments shorter than    1013 bp
  70% of sequence in fragments shorter than    1117 bp
  75% of sequence in fragments shorter than    1244 bp
  80% of sequence in fragments shorter than    1394 bp
  85% of sequence in fragments shorter than    1592 bp
  90% of sequence in fragments shorter than    1869 bp
  95% of sequence in fragments shorter than    2358 bp

# AvaI.HphI (CYCGRG+GGTGA) double digest, 7012 thousand fragments (1005714+6007336 sites), 424 bp average fragment length
   5% of sequence in fragments shorter than     137 bp
  10% of sequence in fragments shorter than     215 bp
  15% of sequence in fragments shorter than     288 bp
  20% of sequence in fragments shorter than     353 bp
  25% of sequence in fragments shorter than     421 bp
  30% of sequence in fragments shorter than     489 bp
  35% of sequence in fragments shorter than     558 bp
  40% of sequence in fragments shorter than     630 bp
  45% of sequence in fragments shorter than     705 bp
  50% of sequence in fragments shorter than     788 bp
  55% of sequence in fragments shorter than     872 bp
  60% of sequence in fragments shorter than     972 bp
  65% of sequence in fragments shorter than    1081 bp
  70% of sequence in fragments shorter than    1208 bp
  75% of sequence in fragments shorter than    1354 bp
  80% of sequence in fragments shorter than    1517 bp
  85% of sequence in fragments shorter than    1748 bp
  90% of sequence in fragments shorter than    2068 bp
  95% of sequence in fragments shorter than    2632 bp

# ApoI.HaeII (RAATTY+RGCGCY) double digest, 7012 thousand fragments (6382433+630101 sites), 424 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     284 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     411 bp
  30% of sequence in fragments shorter than     479 bp
  35% of sequence in fragments shorter than     547 bp
  40% of sequence in fragments shorter than     617 bp
  45% of sequence in fragments shorter than     690 bp
  50% of sequence in fragments shorter than     769 bp
  55% of sequence in fragments shorter than     857 bp
  60% of sequence in fragments shorter than     946 bp
  65% of sequence in fragments shorter than    1050 bp
  70% of sequence in fragments shorter than    1170 bp
  75% of sequence in fragments shorter than    1308 bp
  80% of sequence in fragments shorter than    1468 bp
  85% of sequence in fragments shorter than    1684 bp
  90% of sequence in fragments shorter than    1997 bp
  95% of sequence in fragments shorter than    2555 bp

# BsmAI.SapI (GTCTC+GCTCTTC) double digest, 7008 thousand fragments (6631585+377161 sites), 425 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     430 bp
  30% of sequence in fragments shorter than     503 bp
  35% of sequence in fragments shorter than     575 bp
  40% of sequence in fragments shorter than     648 bp
  45% of sequence in fragments shorter than     729 bp
  50% of sequence in fragments shorter than     818 bp
  55% of sequence in fragments shorter than     912 bp
  60% of sequence in fragments shorter than    1018 bp
  65% of sequence in fragments shorter than    1135 bp
  70% of sequence in fragments shorter than    1270 bp
  75% of sequence in fragments shorter than    1433 bp
  80% of sequence in fragments shorter than    1632 bp
  85% of sequence in fragments shorter than    1893 bp
  90% of sequence in fragments shorter than    2249 bp
  95% of sequence in fragments shorter than    2918 bp

# BsmAI.SmaI (GTCTC+CCCGGG) double digest, 7008 thousand fragments (6631585+376940 sites), 425 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     289 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     441 bp
  30% of sequence in fragments shorter than     517 bp
  35% of sequence in fragments shorter than     590 bp
  40% of sequence in fragments shorter than     670 bp
  45% of sequence in fragments shorter than     756 bp
  50% of sequence in fragments shorter than     850 bp
  55% of sequence in fragments shorter than     950 bp
  60% of sequence in fragments shorter than    1061 bp
  65% of sequence in fragments shorter than    1187 bp
  70% of sequence in fragments shorter than    1332 bp
  75% of sequence in fragments shorter than    1502 bp
  80% of sequence in fragments shorter than    1710 bp
  85% of sequence in fragments shorter than    1982 bp
  90% of sequence in fragments shorter than    2362 bp
  95% of sequence in fragments shorter than    3058 bp

# BamHI.BsmAI (GGATCC+GTCTC) double digest, 6997 thousand fragments (365999+6631585 sites), 425 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     204 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     432 bp
  30% of sequence in fragments shorter than     505 bp
  35% of sequence in fragments shorter than     578 bp
  40% of sequence in fragments shorter than     652 bp
  45% of sequence in fragments shorter than     731 bp
  50% of sequence in fragments shorter than     820 bp
  55% of sequence in fragments shorter than     915 bp
  60% of sequence in fragments shorter than    1022 bp
  65% of sequence in fragments shorter than    1140 bp
  70% of sequence in fragments shorter than    1276 bp
  75% of sequence in fragments shorter than    1434 bp
  80% of sequence in fragments shorter than    1622 bp
  85% of sequence in fragments shorter than    1874 bp
  90% of sequence in fragments shorter than    2218 bp
  95% of sequence in fragments shorter than    2865 bp

# BbvI.BfiI (GCAGC+ACTGGG) double digest, 6996 thousand fragments (5290051+1706594 sites), 425 bp average fragment length
   5% of sequence in fragments shorter than     139 bp
  10% of sequence in fragments shorter than     224 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     377 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     539 bp
  35% of sequence in fragments shorter than     624 bp
  40% of sequence in fragments shorter than     711 bp
  45% of sequence in fragments shorter than     807 bp
  50% of sequence in fragments shorter than     896 bp
  55% of sequence in fragments shorter than    1006 bp
  60% of sequence in fragments shorter than    1125 bp
  65% of sequence in fragments shorter than    1255 bp
  70% of sequence in fragments shorter than    1408 bp
  75% of sequence in fragments shorter than    1591 bp
  80% of sequence in fragments shorter than    1794 bp
  85% of sequence in fragments shorter than    2067 bp
  90% of sequence in fragments shorter than    2462 bp
  95% of sequence in fragments shorter than    3126 bp

# ApoI.SacI (RAATTY+GAGCTC) double digest, 6985 thousand fragments (6382433+603323 sites), 426 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     350 bp
  25% of sequence in fragments shorter than     415 bp
  30% of sequence in fragments shorter than     483 bp
  35% of sequence in fragments shorter than     553 bp
  40% of sequence in fragments shorter than     624 bp
  45% of sequence in fragments shorter than     698 bp
  50% of sequence in fragments shorter than     781 bp
  55% of sequence in fragments shorter than     869 bp
  60% of sequence in fragments shorter than     962 bp
  65% of sequence in fragments shorter than    1068 bp
  70% of sequence in fragments shorter than    1191 bp
  75% of sequence in fragments shorter than    1329 bp
  80% of sequence in fragments shorter than    1501 bp
  85% of sequence in fragments shorter than    1735 bp
  90% of sequence in fragments shorter than    2075 bp
  95% of sequence in fragments shorter than    2708 bp

# BspHI.HphI (TCATGA+GGTGA) double digest, 6985 thousand fragments (978290+6007336 sites), 426 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     220 bp
  15% of sequence in fragments shorter than     287 bp
  20% of sequence in fragments shorter than     348 bp
  25% of sequence in fragments shorter than     410 bp
  30% of sequence in fragments shorter than     473 bp
  35% of sequence in fragments shorter than     535 bp
  40% of sequence in fragments shorter than     599 bp
  45% of sequence in fragments shorter than     668 bp
  50% of sequence in fragments shorter than     738 bp
  55% of sequence in fragments shorter than     813 bp
  60% of sequence in fragments shorter than     898 bp
  65% of sequence in fragments shorter than     993 bp
  70% of sequence in fragments shorter than    1099 bp
  75% of sequence in fragments shorter than    1225 bp
  80% of sequence in fragments shorter than    1374 bp
  85% of sequence in fragments shorter than    1557 bp
  90% of sequence in fragments shorter than    1832 bp
  95% of sequence in fragments shorter than    2297 bp

# BglII.BthCI (AGATCT+GCNGC) double digest, 6984 thousand fragments (774732+6209929 sites), 426 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     319 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     486 bp
  30% of sequence in fragments shorter than     570 bp
  35% of sequence in fragments shorter than     658 bp
  40% of sequence in fragments shorter than     748 bp
  45% of sequence in fragments shorter than     844 bp
  50% of sequence in fragments shorter than     945 bp
  55% of sequence in fragments shorter than    1055 bp
  60% of sequence in fragments shorter than    1178 bp
  65% of sequence in fragments shorter than    1313 bp
  70% of sequence in fragments shorter than    1465 bp
  75% of sequence in fragments shorter than    1636 bp
  80% of sequence in fragments shorter than    1855 bp
  85% of sequence in fragments shorter than    2135 bp
  90% of sequence in fragments shorter than    2530 bp
  95% of sequence in fragments shorter than    3194 bp

# ApoI.AvrII (RAATTY+CCTAGG) double digest, 6976 thousand fragments (6382433+594956 sites), 427 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     284 bp
  20% of sequence in fragments shorter than     349 bp
  25% of sequence in fragments shorter than     415 bp
  30% of sequence in fragments shorter than     484 bp
  35% of sequence in fragments shorter than     552 bp
  40% of sequence in fragments shorter than     625 bp
  45% of sequence in fragments shorter than     698 bp
  50% of sequence in fragments shorter than     782 bp
  55% of sequence in fragments shorter than     871 bp
  60% of sequence in fragments shorter than     966 bp
  65% of sequence in fragments shorter than    1076 bp
  70% of sequence in fragments shorter than    1204 bp
  75% of sequence in fragments shorter than    1355 bp
  80% of sequence in fragments shorter than    1539 bp
  85% of sequence in fragments shorter than    1790 bp
  90% of sequence in fragments shorter than    2155 bp
  95% of sequence in fragments shorter than    2869 bp

# BscGI.BsrI (CCCGT+ACTGG) double digest, 6971 thousand fragments (1231070+5741310 sites), 427 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     220 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     346 bp
  25% of sequence in fragments shorter than     407 bp
  30% of sequence in fragments shorter than     471 bp
  35% of sequence in fragments shorter than     535 bp
  40% of sequence in fragments shorter than     604 bp
  45% of sequence in fragments shorter than     675 bp
  50% of sequence in fragments shorter than     740 bp
  55% of sequence in fragments shorter than     822 bp
  60% of sequence in fragments shorter than     905 bp
  65% of sequence in fragments shorter than    1004 bp
  70% of sequence in fragments shorter than    1115 bp
  75% of sequence in fragments shorter than    1243 bp
  80% of sequence in fragments shorter than    1398 bp
  85% of sequence in fragments shorter than    1595 bp
  90% of sequence in fragments shorter than    1872 bp
  95% of sequence in fragments shorter than    2339 bp

# BthCI.NcoI (GCNGC+CCATGG) double digest, 6969 thousand fragments (6209929+759594 sites), 427 bp average fragment length
   5% of sequence in fragments shorter than     140 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     409 bp
  25% of sequence in fragments shorter than     498 bp
  30% of sequence in fragments shorter than     590 bp
  35% of sequence in fragments shorter than     686 bp
  40% of sequence in fragments shorter than     787 bp
  45% of sequence in fragments shorter than     888 bp
  50% of sequence in fragments shorter than    1003 bp
  55% of sequence in fragments shorter than    1122 bp
  60% of sequence in fragments shorter than    1258 bp
  65% of sequence in fragments shorter than    1407 bp
  70% of sequence in fragments shorter than    1584 bp
  75% of sequence in fragments shorter than    1777 bp
  80% of sequence in fragments shorter than    2024 bp
  85% of sequence in fragments shorter than    2327 bp
  90% of sequence in fragments shorter than    2765 bp
  95% of sequence in fragments shorter than    3517 bp

# BsmAI.BstEII (GTCTC+GGTNACC) double digest, 6966 thousand fragments (6631585+335865 sites), 427 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     205 bp
  15% of sequence in fragments shorter than     286 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     505 bp
  35% of sequence in fragments shorter than     577 bp
  40% of sequence in fragments shorter than     652 bp
  45% of sequence in fragments shorter than     735 bp
  50% of sequence in fragments shorter than     824 bp
  55% of sequence in fragments shorter than     920 bp
  60% of sequence in fragments shorter than    1026 bp
  65% of sequence in fragments shorter than    1144 bp
  70% of sequence in fragments shorter than    1280 bp
  75% of sequence in fragments shorter than    1444 bp
  80% of sequence in fragments shorter than    1647 bp
  85% of sequence in fragments shorter than    1909 bp
  90% of sequence in fragments shorter than    2274 bp
  95% of sequence in fragments shorter than    2951 bp

# FokI.HhaI (GGATG+GCGC) double digest, 6957 thousand fragments (5201116+1756502 sites), 428 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     441 bp
  30% of sequence in fragments shorter than     515 bp
  35% of sequence in fragments shorter than     590 bp
  40% of sequence in fragments shorter than     669 bp
  45% of sequence in fragments shorter than     747 bp
  50% of sequence in fragments shorter than     835 bp
  55% of sequence in fragments shorter than     928 bp
  60% of sequence in fragments shorter than    1027 bp
  65% of sequence in fragments shorter than    1138 bp
  70% of sequence in fragments shorter than    1265 bp
  75% of sequence in fragments shorter than    1408 bp
  80% of sequence in fragments shorter than    1587 bp
  85% of sequence in fragments shorter than    1818 bp
  90% of sequence in fragments shorter than    2143 bp
  95% of sequence in fragments shorter than    2699 bp

# ApoI.HgaI (RAATTY+GACGC) double digest, 6953 thousand fragments (6382433+571889 sites), 428 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     221 bp
  15% of sequence in fragments shorter than     287 bp
  20% of sequence in fragments shorter than     352 bp
  25% of sequence in fragments shorter than     418 bp
  30% of sequence in fragments shorter than     487 bp
  35% of sequence in fragments shorter than     557 bp
  40% of sequence in fragments shorter than     628 bp
  45% of sequence in fragments shorter than     703 bp
  50% of sequence in fragments shorter than     785 bp
  55% of sequence in fragments shorter than     871 bp
  60% of sequence in fragments shorter than     963 bp
  65% of sequence in fragments shorter than    1070 bp
  70% of sequence in fragments shorter than    1192 bp
  75% of sequence in fragments shorter than    1327 bp
  80% of sequence in fragments shorter than    1494 bp
  85% of sequence in fragments shorter than    1721 bp
  90% of sequence in fragments shorter than    2045 bp
  95% of sequence in fragments shorter than    2625 bp

# AciI.AvaII (CCGC+GGWCC) double digest, 6953 thousand fragments (4206130+2747690 sites), 428 bp average fragment length
   5% of sequence in fragments shorter than     138 bp
  10% of sequence in fragments shorter than     231 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     421 bp
  25% of sequence in fragments shorter than     520 bp
  30% of sequence in fragments shorter than     622 bp
  35% of sequence in fragments shorter than     725 bp
  40% of sequence in fragments shorter than     840 bp
  45% of sequence in fragments shorter than     949 bp
  50% of sequence in fragments shorter than    1077 bp
  55% of sequence in fragments shorter than    1219 bp
  60% of sequence in fragments shorter than    1375 bp
  65% of sequence in fragments shorter than    1550 bp
  70% of sequence in fragments shorter than    1754 bp
  75% of sequence in fragments shorter than    1994 bp
  80% of sequence in fragments shorter than    2293 bp
  85% of sequence in fragments shorter than    2676 bp
  90% of sequence in fragments shorter than    3218 bp
  95% of sequence in fragments shorter than    4167 bp

# ApoI.EciI (RAATTY+GGCGGA) double digest, 6953 thousand fragments (6382433+571273 sites), 428 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     220 bp
  15% of sequence in fragments shorter than     283 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     411 bp
  30% of sequence in fragments shorter than     479 bp
  35% of sequence in fragments shorter than     548 bp
  40% of sequence in fragments shorter than     619 bp
  45% of sequence in fragments shorter than     693 bp
  50% of sequence in fragments shorter than     774 bp
  55% of sequence in fragments shorter than     861 bp
  60% of sequence in fragments shorter than     954 bp
  65% of sequence in fragments shorter than    1062 bp
  70% of sequence in fragments shorter than    1185 bp
  75% of sequence in fragments shorter than    1323 bp
  80% of sequence in fragments shorter than    1497 bp
  85% of sequence in fragments shorter than    1733 bp
  90% of sequence in fragments shorter than    2078 bp
  95% of sequence in fragments shorter than    2717 bp

# BclI.BthCI (TGATCA+GCNGC) double digest, 6948 thousand fragments (738785+6209929 sites), 428 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     320 bp
  20% of sequence in fragments shorter than     403 bp
  25% of sequence in fragments shorter than     489 bp
  30% of sequence in fragments shorter than     574 bp
  35% of sequence in fragments shorter than     663 bp
  40% of sequence in fragments shorter than     756 bp
  45% of sequence in fragments shorter than     855 bp
  50% of sequence in fragments shorter than     959 bp
  55% of sequence in fragments shorter than    1067 bp
  60% of sequence in fragments shorter than    1194 bp
  65% of sequence in fragments shorter than    1331 bp
  70% of sequence in fragments shorter than    1487 bp
  75% of sequence in fragments shorter than    1663 bp
  80% of sequence in fragments shorter than    1882 bp
  85% of sequence in fragments shorter than    2149 bp
  90% of sequence in fragments shorter than    2544 bp
  95% of sequence in fragments shorter than    3213 bp

# ApoI.MfeI (RAATTY+CAATTG) double digest, 6946 thousand fragments (6382433+564303 sites), 429 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     285 bp
  20% of sequence in fragments shorter than     352 bp
  25% of sequence in fragments shorter than     419 bp
  30% of sequence in fragments shorter than     487 bp
  35% of sequence in fragments shorter than     560 bp
  40% of sequence in fragments shorter than     635 bp
  45% of sequence in fragments shorter than     714 bp
  50% of sequence in fragments shorter than     802 bp
  55% of sequence in fragments shorter than     890 bp
  60% of sequence in fragments shorter than     992 bp
  65% of sequence in fragments shorter than    1110 bp
  70% of sequence in fragments shorter than    1247 bp
  75% of sequence in fragments shorter than    1408 bp
  80% of sequence in fragments shorter than    1618 bp
  85% of sequence in fragments shorter than    1901 bp
  90% of sequence in fragments shorter than    2334 bp
  95% of sequence in fragments shorter than    3241 bp

# BccI.BglII (CCATC+AGATCT) double digest, 6945 thousand fragments (6170927+774732 sites), 429 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     293 bp
  20% of sequence in fragments shorter than     355 bp
  25% of sequence in fragments shorter than     418 bp
  30% of sequence in fragments shorter than     480 bp
  35% of sequence in fragments shorter than     547 bp
  40% of sequence in fragments shorter than     616 bp
  45% of sequence in fragments shorter than     686 bp
  50% of sequence in fragments shorter than     761 bp
  55% of sequence in fragments shorter than     843 bp
  60% of sequence in fragments shorter than     933 bp
  65% of sequence in fragments shorter than    1034 bp
  70% of sequence in fragments shorter than    1145 bp
  75% of sequence in fragments shorter than    1278 bp
  80% of sequence in fragments shorter than    1442 bp
  85% of sequence in fragments shorter than    1654 bp
  90% of sequence in fragments shorter than    1961 bp
  95% of sequence in fragments shorter than    2536 bp

# BthCI.FnuDII (GCNGC+CGCG) double digest, 6943 thousand fragments (6209929+733940 sites), 429 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     248 bp
  15% of sequence in fragments shorter than     338 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     524 bp
  30% of sequence in fragments shorter than     619 bp
  35% of sequence in fragments shorter than     719 bp
  40% of sequence in fragments shorter than     825 bp
  45% of sequence in fragments shorter than     932 bp
  50% of sequence in fragments shorter than    1048 bp
  55% of sequence in fragments shorter than    1180 bp
  60% of sequence in fragments shorter than    1322 bp
  65% of sequence in fragments shorter than    1482 bp
  70% of sequence in fragments shorter than    1659 bp
  75% of sequence in fragments shorter than    1873 bp
  80% of sequence in fragments shorter than    2120 bp
  85% of sequence in fragments shorter than    2459 bp
  90% of sequence in fragments shorter than    2929 bp
  95% of sequence in fragments shorter than    3734 bp

# AvaIII.HphI (ATGCAT+GGTGA) double digest, 6940 thousand fragments (933357+6007336 sites), 429 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     221 bp
  15% of sequence in fragments shorter than     289 bp
  20% of sequence in fragments shorter than     349 bp
  25% of sequence in fragments shorter than     411 bp
  30% of sequence in fragments shorter than     473 bp
  35% of sequence in fragments shorter than     536 bp
  40% of sequence in fragments shorter than     599 bp
  45% of sequence in fragments shorter than     667 bp
  50% of sequence in fragments shorter than     737 bp
  55% of sequence in fragments shorter than     812 bp
  60% of sequence in fragments shorter than     896 bp
  65% of sequence in fragments shorter than     991 bp
  70% of sequence in fragments shorter than    1097 bp
  75% of sequence in fragments shorter than    1225 bp
  80% of sequence in fragments shorter than    1372 bp
  85% of sequence in fragments shorter than    1551 bp
  90% of sequence in fragments shorter than    1825 bp
  95% of sequence in fragments shorter than    2297 bp

# BbvI.PsiI (GCAGC+TTATAA) double digest, 6937 thousand fragments (5290051+1647912 sites), 429 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     374 bp
  25% of sequence in fragments shorter than     445 bp
  30% of sequence in fragments shorter than     518 bp
  35% of sequence in fragments shorter than     591 bp
  40% of sequence in fragments shorter than     667 bp
  45% of sequence in fragments shorter than     747 bp
  50% of sequence in fragments shorter than     831 bp
  55% of sequence in fragments shorter than     919 bp
  60% of sequence in fragments shorter than    1015 bp
  65% of sequence in fragments shorter than    1122 bp
  70% of sequence in fragments shorter than    1246 bp
  75% of sequence in fragments shorter than    1388 bp
  80% of sequence in fragments shorter than    1565 bp
  85% of sequence in fragments shorter than    1774 bp
  90% of sequence in fragments shorter than    2077 bp
  95% of sequence in fragments shorter than    2621 bp

# AciI.SmlI (CCGC+CTYRAG) double digest, 6933 thousand fragments (4206130+2727867 sites), 429 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     231 bp
  15% of sequence in fragments shorter than     323 bp
  20% of sequence in fragments shorter than     407 bp
  25% of sequence in fragments shorter than     492 bp
  30% of sequence in fragments shorter than     577 bp
  35% of sequence in fragments shorter than     666 bp
  40% of sequence in fragments shorter than     757 bp
  45% of sequence in fragments shorter than     855 bp
  50% of sequence in fragments shorter than     958 bp
  55% of sequence in fragments shorter than    1066 bp
  60% of sequence in fragments shorter than    1193 bp
  65% of sequence in fragments shorter than    1333 bp
  70% of sequence in fragments shorter than    1486 bp
  75% of sequence in fragments shorter than    1675 bp
  80% of sequence in fragments shorter than    1907 bp
  85% of sequence in fragments shorter than    2203 bp
  90% of sequence in fragments shorter than    2614 bp
  95% of sequence in fragments shorter than    3324 bp

# ApoI.SphI (RAATTY+GCATGC) double digest, 6932 thousand fragments (6382433+550952 sites), 429 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     220 bp
  15% of sequence in fragments shorter than     286 bp
  20% of sequence in fragments shorter than     350 bp
  25% of sequence in fragments shorter than     416 bp
  30% of sequence in fragments shorter than     485 bp
  35% of sequence in fragments shorter than     556 bp
  40% of sequence in fragments shorter than     628 bp
  45% of sequence in fragments shorter than     704 bp
  50% of sequence in fragments shorter than     788 bp
  55% of sequence in fragments shorter than     877 bp
  60% of sequence in fragments shorter than     973 bp
  65% of sequence in fragments shorter than    1084 bp
  70% of sequence in fragments shorter than    1213 bp
  75% of sequence in fragments shorter than    1358 bp
  80% of sequence in fragments shorter than    1542 bp
  85% of sequence in fragments shorter than    1793 bp
  90% of sequence in fragments shorter than    2162 bp
  95% of sequence in fragments shorter than    2894 bp

# HphI.StuI (GGTGA+AGGCCT) double digest, 6932 thousand fragments (6007336+925729 sites), 429 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     221 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     347 bp
  25% of sequence in fragments shorter than     410 bp
  30% of sequence in fragments shorter than     475 bp
  35% of sequence in fragments shorter than     539 bp
  40% of sequence in fragments shorter than     606 bp
  45% of sequence in fragments shorter than     677 bp
  50% of sequence in fragments shorter than     749 bp
  55% of sequence in fragments shorter than     832 bp
  60% of sequence in fragments shorter than     923 bp
  65% of sequence in fragments shorter than    1027 bp
  70% of sequence in fragments shorter than    1143 bp
  75% of sequence in fragments shorter than    1277 bp
  80% of sequence in fragments shorter than    1429 bp
  85% of sequence in fragments shorter than    1638 bp
  90% of sequence in fragments shorter than    1933 bp
  95% of sequence in fragments shorter than    2425 bp

# BccI.NcoI (CCATC+CCATGG) double digest, 6930 thousand fragments (6170927+759594 sites), 430 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     292 bp
  20% of sequence in fragments shorter than     356 bp
  25% of sequence in fragments shorter than     422 bp
  30% of sequence in fragments shorter than     486 bp
  35% of sequence in fragments shorter than     555 bp
  40% of sequence in fragments shorter than     625 bp
  45% of sequence in fragments shorter than     700 bp
  50% of sequence in fragments shorter than     779 bp
  55% of sequence in fragments shorter than     865 bp
  60% of sequence in fragments shorter than     961 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1181 bp
  75% of sequence in fragments shorter than    1321 bp
  80% of sequence in fragments shorter than    1491 bp
  85% of sequence in fragments shorter than    1715 bp
  90% of sequence in fragments shorter than    2039 bp
  95% of sequence in fragments shorter than    2633 bp

# BsmAI.SexAI (GTCTC+ACCWGGT) double digest, 6929 thousand fragments (6631585+297900 sites), 430 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     206 bp
  15% of sequence in fragments shorter than     288 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     437 bp
  30% of sequence in fragments shorter than     511 bp
  35% of sequence in fragments shorter than     583 bp
  40% of sequence in fragments shorter than     660 bp
  45% of sequence in fragments shorter than     744 bp
  50% of sequence in fragments shorter than     835 bp
  55% of sequence in fragments shorter than     933 bp
  60% of sequence in fragments shorter than    1041 bp
  65% of sequence in fragments shorter than    1162 bp
  70% of sequence in fragments shorter than    1302 bp
  75% of sequence in fragments shorter than    1469 bp
  80% of sequence in fragments shorter than    1666 bp
  85% of sequence in fragments shorter than    1930 bp
  90% of sequence in fragments shorter than    2294 bp
  95% of sequence in fragments shorter than    2977 bp

# ApoI.ScaI (RAATTY+AGTACT) double digest, 6925 thousand fragments (6382433+543793 sites), 430 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     219 bp
  15% of sequence in fragments shorter than     286 bp
  20% of sequence in fragments shorter than     352 bp
  25% of sequence in fragments shorter than     419 bp
  30% of sequence in fragments shorter than     490 bp
  35% of sequence in fragments shorter than     562 bp
  40% of sequence in fragments shorter than     637 bp
  45% of sequence in fragments shorter than     715 bp
  50% of sequence in fragments shorter than     804 bp
  55% of sequence in fragments shorter than     894 bp
  60% of sequence in fragments shorter than     998 bp
  65% of sequence in fragments shorter than    1116 bp
  70% of sequence in fragments shorter than    1254 bp
  75% of sequence in fragments shorter than    1405 bp
  80% of sequence in fragments shorter than    1614 bp
  85% of sequence in fragments shorter than    1896 bp
  90% of sequence in fragments shorter than    2325 bp
  95% of sequence in fragments shorter than    3213 bp

# BsaAI.BthCI (YACGTR+GCNGC) double digest, 6923 thousand fragments (713801+6209929 sites), 430 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     323 bp
  20% of sequence in fragments shorter than     408 bp
  25% of sequence in fragments shorter than     496 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     676 bp
  40% of sequence in fragments shorter than     774 bp
  45% of sequence in fragments shorter than     873 bp
  50% of sequence in fragments shorter than     983 bp
  55% of sequence in fragments shorter than    1099 bp
  60% of sequence in fragments shorter than    1232 bp
  65% of sequence in fragments shorter than    1379 bp
  70% of sequence in fragments shorter than    1543 bp
  75% of sequence in fragments shorter than    1731 bp
  80% of sequence in fragments shorter than    1966 bp
  85% of sequence in fragments shorter than    2260 bp
  90% of sequence in fragments shorter than    2686 bp
  95% of sequence in fragments shorter than    3407 bp

# BsmAI.KpnI (GTCTC+GGTACC) double digest, 6919 thousand fragments (6631585+287974 sites), 430 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     289 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     436 bp
  30% of sequence in fragments shorter than     508 bp
  35% of sequence in fragments shorter than     581 bp
  40% of sequence in fragments shorter than     657 bp
  45% of sequence in fragments shorter than     740 bp
  50% of sequence in fragments shorter than     830 bp
  55% of sequence in fragments shorter than     927 bp
  60% of sequence in fragments shorter than    1036 bp
  65% of sequence in fragments shorter than    1155 bp
  70% of sequence in fragments shorter than    1291 bp
  75% of sequence in fragments shorter than    1453 bp
  80% of sequence in fragments shorter than    1645 bp
  85% of sequence in fragments shorter than    1895 bp
  90% of sequence in fragments shorter than    2252 bp
  95% of sequence in fragments shorter than    2922 bp

# BsmAI.NheI (GTCTC+GCTAGC) double digest, 6918 thousand fragments (6631585+287384 sites), 430 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     207 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     439 bp
  30% of sequence in fragments shorter than     512 bp
  35% of sequence in fragments shorter than     583 bp
  40% of sequence in fragments shorter than     657 bp
  45% of sequence in fragments shorter than     740 bp
  50% of sequence in fragments shorter than     829 bp
  55% of sequence in fragments shorter than     926 bp
  60% of sequence in fragments shorter than    1032 bp
  65% of sequence in fragments shorter than    1150 bp
  70% of sequence in fragments shorter than    1287 bp
  75% of sequence in fragments shorter than    1444 bp
  80% of sequence in fragments shorter than    1629 bp
  85% of sequence in fragments shorter than    1876 bp
  90% of sequence in fragments shorter than    2220 bp
  95% of sequence in fragments shorter than    2869 bp

# BceAI.BthCI (ACGGC+GCNGC) double digest, 6914 thousand fragments (705178+6209929 sites), 430 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     329 bp
  20% of sequence in fragments shorter than     418 bp
  25% of sequence in fragments shorter than     512 bp
  30% of sequence in fragments shorter than     606 bp
  35% of sequence in fragments shorter than     703 bp
  40% of sequence in fragments shorter than     809 bp
  45% of sequence in fragments shorter than     912 bp
  50% of sequence in fragments shorter than    1030 bp
  55% of sequence in fragments shorter than    1154 bp
  60% of sequence in fragments shorter than    1293 bp
  65% of sequence in fragments shorter than    1449 bp
  70% of sequence in fragments shorter than    1625 bp
  75% of sequence in fragments shorter than    1831 bp
  80% of sequence in fragments shorter than    2074 bp
  85% of sequence in fragments shorter than    2400 bp
  90% of sequence in fragments shorter than    2850 bp
  95% of sequence in fragments shorter than    3640 bp

# HphI.NdeI (GGTGA+CATATG) double digest, 6910 thousand fragments (6007336+903360 sites), 431 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     350 bp
  25% of sequence in fragments shorter than     411 bp
  30% of sequence in fragments shorter than     473 bp
  35% of sequence in fragments shorter than     534 bp
  40% of sequence in fragments shorter than     594 bp
  45% of sequence in fragments shorter than     662 bp
  50% of sequence in fragments shorter than     731 bp
  55% of sequence in fragments shorter than     806 bp
  60% of sequence in fragments shorter than     887 bp
  65% of sequence in fragments shorter than     982 bp
  70% of sequence in fragments shorter than    1087 bp
  75% of sequence in fragments shorter than    1209 bp
  80% of sequence in fragments shorter than    1357 bp
  85% of sequence in fragments shorter than    1532 bp
  90% of sequence in fragments shorter than    1798 bp
  95% of sequence in fragments shorter than    2245 bp

# BccI.BclI (CCATC+TGATCA) double digest, 6909 thousand fragments (6170927+738785 sites), 431 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     224 bp
  15% of sequence in fragments shorter than     293 bp
  20% of sequence in fragments shorter than     355 bp
  25% of sequence in fragments shorter than     419 bp
  30% of sequence in fragments shorter than     482 bp
  35% of sequence in fragments shorter than     550 bp
  40% of sequence in fragments shorter than     619 bp
  45% of sequence in fragments shorter than     690 bp
  50% of sequence in fragments shorter than     767 bp
  55% of sequence in fragments shorter than     849 bp
  60% of sequence in fragments shorter than     940 bp
  65% of sequence in fragments shorter than    1042 bp
  70% of sequence in fragments shorter than    1151 bp
  75% of sequence in fragments shorter than    1284 bp
  80% of sequence in fragments shorter than    1448 bp
  85% of sequence in fragments shorter than    1660 bp
  90% of sequence in fragments shorter than    1966 bp
  95% of sequence in fragments shorter than    2542 bp

# BfiI.FokI (ACTGGG+GGATG) double digest, 6907 thousand fragments (1706594+5201116 sites), 431 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     296 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     498 bp
  35% of sequence in fragments shorter than     568 bp
  40% of sequence in fragments shorter than     638 bp
  45% of sequence in fragments shorter than     714 bp
  50% of sequence in fragments shorter than     793 bp
  55% of sequence in fragments shorter than     881 bp
  60% of sequence in fragments shorter than     971 bp
  65% of sequence in fragments shorter than    1075 bp
  70% of sequence in fragments shorter than    1192 bp
  75% of sequence in fragments shorter than    1331 bp
  80% of sequence in fragments shorter than    1494 bp
  85% of sequence in fragments shorter than    1713 bp
  90% of sequence in fragments shorter than    2011 bp
  95% of sequence in fragments shorter than    2553 bp

# BciVI.BthCI (GTATCC+GCNGC) double digest, 6905 thousand fragments (696093+6209929 sites), 431 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     405 bp
  25% of sequence in fragments shorter than     491 bp
  30% of sequence in fragments shorter than     575 bp
  35% of sequence in fragments shorter than     664 bp
  40% of sequence in fragments shorter than     758 bp
  45% of sequence in fragments shorter than     856 bp
  50% of sequence in fragments shorter than     956 bp
  55% of sequence in fragments shorter than    1069 bp
  60% of sequence in fragments shorter than    1192 bp
  65% of sequence in fragments shorter than    1330 bp
  70% of sequence in fragments shorter than    1484 bp
  75% of sequence in fragments shorter than    1670 bp
  80% of sequence in fragments shorter than    1894 bp
  85% of sequence in fragments shorter than    2171 bp
  90% of sequence in fragments shorter than    2574 bp
  95% of sequence in fragments shorter than    3246 bp

# BccI.FnuDII (CCATC+CGCG) double digest, 6904 thousand fragments (6170927+733940 sites), 431 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     435 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     578 bp
  40% of sequence in fragments shorter than     652 bp
  45% of sequence in fragments shorter than     731 bp
  50% of sequence in fragments shorter than     816 bp
  55% of sequence in fragments shorter than     907 bp
  60% of sequence in fragments shorter than    1009 bp
  65% of sequence in fragments shorter than    1116 bp
  70% of sequence in fragments shorter than    1243 bp
  75% of sequence in fragments shorter than    1395 bp
  80% of sequence in fragments shorter than    1582 bp
  85% of sequence in fragments shorter than    1823 bp
  90% of sequence in fragments shorter than    2178 bp
  95% of sequence in fragments shorter than    2834 bp

# BbvI.EsaBC3I (GCAGC+TCGA) double digest, 6892 thousand fragments (5290051+1603346 sites), 432 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     450 bp
  30% of sequence in fragments shorter than     527 bp
  35% of sequence in fragments shorter than     607 bp
  40% of sequence in fragments shorter than     689 bp
  45% of sequence in fragments shorter than     777 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     974 bp
  60% of sequence in fragments shorter than    1085 bp
  65% of sequence in fragments shorter than    1210 bp
  70% of sequence in fragments shorter than    1356 bp
  75% of sequence in fragments shorter than    1521 bp
  80% of sequence in fragments shorter than    1713 bp
  85% of sequence in fragments shorter than    1966 bp
  90% of sequence in fragments shorter than    2323 bp
  95% of sequence in fragments shorter than    2951 bp

# BccI.BsaAI (CCATC+YACGTR) double digest, 6884 thousand fragments (6170927+713801 sites), 432 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     425 bp
  30% of sequence in fragments shorter than     489 bp
  35% of sequence in fragments shorter than     557 bp
  40% of sequence in fragments shorter than     627 bp
  45% of sequence in fragments shorter than     700 bp
  50% of sequence in fragments shorter than     778 bp
  55% of sequence in fragments shorter than     862 bp
  60% of sequence in fragments shorter than     955 bp
  65% of sequence in fragments shorter than    1056 bp
  70% of sequence in fragments shorter than    1168 bp
  75% of sequence in fragments shorter than    1304 bp
  80% of sequence in fragments shorter than    1470 bp
  85% of sequence in fragments shorter than    1686 bp
  90% of sequence in fragments shorter than    1993 bp
  95% of sequence in fragments shorter than    2542 bp

# ApaLI.ApoI (GTGCAC+RAATTY) double digest, 6878 thousand fragments (496255+6382433 sites), 433 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     289 bp
  20% of sequence in fragments shorter than     354 bp
  25% of sequence in fragments shorter than     421 bp
  30% of sequence in fragments shorter than     491 bp
  35% of sequence in fragments shorter than     563 bp
  40% of sequence in fragments shorter than     635 bp
  45% of sequence in fragments shorter than     712 bp
  50% of sequence in fragments shorter than     797 bp
  55% of sequence in fragments shorter than     880 bp
  60% of sequence in fragments shorter than     977 bp
  65% of sequence in fragments shorter than    1089 bp
  70% of sequence in fragments shorter than    1220 bp
  75% of sequence in fragments shorter than    1366 bp
  80% of sequence in fragments shorter than    1549 bp
  85% of sequence in fragments shorter than    1800 bp
  90% of sequence in fragments shorter than    2165 bp
  95% of sequence in fragments shorter than    2871 bp

# BccI.BceAI (CCATC+ACGGC) double digest, 6875 thousand fragments (6170927+705178 sites), 433 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     224 bp
  15% of sequence in fragments shorter than     294 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     425 bp
  30% of sequence in fragments shorter than     489 bp
  35% of sequence in fragments shorter than     559 bp
  40% of sequence in fragments shorter than     630 bp
  45% of sequence in fragments shorter than     705 bp
  50% of sequence in fragments shorter than     787 bp
  55% of sequence in fragments shorter than     874 bp
  60% of sequence in fragments shorter than     971 bp
  65% of sequence in fragments shorter than    1075 bp
  70% of sequence in fragments shorter than    1197 bp
  75% of sequence in fragments shorter than    1339 bp
  80% of sequence in fragments shorter than    1518 bp
  85% of sequence in fragments shorter than    1746 bp
  90% of sequence in fragments shorter than    2081 bp
  95% of sequence in fragments shorter than    2700 bp

# CviQI.HhaI (GTAC+GCGC) double digest, 6871 thousand fragments (5115078+1756502 sites), 433 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     233 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     370 bp
  25% of sequence in fragments shorter than     436 bp
  30% of sequence in fragments shorter than     501 bp
  35% of sequence in fragments shorter than     567 bp
  40% of sequence in fragments shorter than     637 bp
  45% of sequence in fragments shorter than     705 bp
  50% of sequence in fragments shorter than     779 bp
  55% of sequence in fragments shorter than     862 bp
  60% of sequence in fragments shorter than     948 bp
  65% of sequence in fragments shorter than    1043 bp
  70% of sequence in fragments shorter than    1155 bp
  75% of sequence in fragments shorter than    1276 bp
  80% of sequence in fragments shorter than    1413 bp
  85% of sequence in fragments shorter than    1601 bp
  90% of sequence in fragments shorter than    1858 bp
  95% of sequence in fragments shorter than    2301 bp

# HindIII.HphI (AAGCTT+GGTGA) double digest, 6867 thousand fragments (860365+6007336 sites), 433 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     222 bp
  15% of sequence in fragments shorter than     292 bp
  20% of sequence in fragments shorter than     351 bp
  25% of sequence in fragments shorter than     414 bp
  30% of sequence in fragments shorter than     477 bp
  35% of sequence in fragments shorter than     539 bp
  40% of sequence in fragments shorter than     604 bp
  45% of sequence in fragments shorter than     674 bp
  50% of sequence in fragments shorter than     746 bp
  55% of sequence in fragments shorter than     826 bp
  60% of sequence in fragments shorter than     912 bp
  65% of sequence in fragments shorter than    1011 bp
  70% of sequence in fragments shorter than    1121 bp
  75% of sequence in fragments shorter than    1247 bp
  80% of sequence in fragments shorter than    1391 bp
  85% of sequence in fragments shorter than    1588 bp
  90% of sequence in fragments shorter than    1872 bp
  95% of sequence in fragments shorter than    2344 bp

# BsmAI.BsrBI (GTCTC+CCGCTC) double digest, 6866 thousand fragments (6631585+235523 sites), 433 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     208 bp
  15% of sequence in fragments shorter than     291 bp
  20% of sequence in fragments shorter than     367 bp
  25% of sequence in fragments shorter than     442 bp
  30% of sequence in fragments shorter than     517 bp
  35% of sequence in fragments shorter than     590 bp
  40% of sequence in fragments shorter than     669 bp
  45% of sequence in fragments shorter than     755 bp
  50% of sequence in fragments shorter than     848 bp
  55% of sequence in fragments shorter than     947 bp
  60% of sequence in fragments shorter than    1057 bp
  65% of sequence in fragments shorter than    1181 bp
  70% of sequence in fragments shorter than    1325 bp
  75% of sequence in fragments shorter than    1494 bp
  80% of sequence in fragments shorter than    1698 bp
  85% of sequence in fragments shorter than    1966 bp
  90% of sequence in fragments shorter than    2341 bp
  95% of sequence in fragments shorter than    3028 bp

# BccI.BciVI (CCATC+GTATCC) double digest, 6866 thousand fragments (6170927+696093 sites), 433 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     358 bp
  25% of sequence in fragments shorter than     423 bp
  30% of sequence in fragments shorter than     487 bp
  35% of sequence in fragments shorter than     555 bp
  40% of sequence in fragments shorter than     626 bp
  45% of sequence in fragments shorter than     699 bp
  50% of sequence in fragments shorter than     775 bp
  55% of sequence in fragments shorter than     859 bp
  60% of sequence in fragments shorter than     950 bp
  65% of sequence in fragments shorter than    1053 bp
  70% of sequence in fragments shorter than    1166 bp
  75% of sequence in fragments shorter than    1301 bp
  80% of sequence in fragments shorter than    1468 bp
  85% of sequence in fragments shorter than    1683 bp
  90% of sequence in fragments shorter than    1993 bp
  95% of sequence in fragments shorter than    2570 bp

# BsmAI.KasI (GTCTC+GGCGCC) double digest, 6861 thousand fragments (6631585+230381 sites), 434 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     449 bp
  30% of sequence in fragments shorter than     523 bp
  35% of sequence in fragments shorter than     599 bp
  40% of sequence in fragments shorter than     680 bp
  45% of sequence in fragments shorter than     767 bp
  50% of sequence in fragments shorter than     861 bp
  55% of sequence in fragments shorter than     963 bp
  60% of sequence in fragments shorter than    1074 bp
  65% of sequence in fragments shorter than    1200 bp
  70% of sequence in fragments shorter than    1347 bp
  75% of sequence in fragments shorter than    1515 bp
  80% of sequence in fragments shorter than    1725 bp
  85% of sequence in fragments shorter than    1997 bp
  90% of sequence in fragments shorter than    2377 bp
  95% of sequence in fragments shorter than    3075 bp

# HphI.XbaI (GGTGA+TCTAGA) double digest, 6857 thousand fragments (6007336+850998 sites), 434 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     221 bp
  15% of sequence in fragments shorter than     291 bp
  20% of sequence in fragments shorter than     353 bp
  25% of sequence in fragments shorter than     416 bp
  30% of sequence in fragments shorter than     480 bp
  35% of sequence in fragments shorter than     543 bp
  40% of sequence in fragments shorter than     607 bp
  45% of sequence in fragments shorter than     675 bp
  50% of sequence in fragments shorter than     744 bp
  55% of sequence in fragments shorter than     822 bp
  60% of sequence in fragments shorter than     909 bp
  65% of sequence in fragments shorter than    1005 bp
  70% of sequence in fragments shorter than    1114 bp
  75% of sequence in fragments shorter than    1240 bp
  80% of sequence in fragments shorter than    1383 bp
  85% of sequence in fragments shorter than    1568 bp
  90% of sequence in fragments shorter than    1844 bp
  95% of sequence in fragments shorter than    2310 bp

# BsmAI.SwaI (GTCTC+ATTTAAAT) double digest, 6857 thousand fragments (6631585+226602 sites), 434 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     209 bp
  15% of sequence in fragments shorter than     292 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     441 bp
  30% of sequence in fragments shorter than     515 bp
  35% of sequence in fragments shorter than     586 bp
  40% of sequence in fragments shorter than     662 bp
  45% of sequence in fragments shorter than     745 bp
  50% of sequence in fragments shorter than     835 bp
  55% of sequence in fragments shorter than     930 bp
  60% of sequence in fragments shorter than    1037 bp
  65% of sequence in fragments shorter than    1154 bp
  70% of sequence in fragments shorter than    1290 bp
  75% of sequence in fragments shorter than    1452 bp
  80% of sequence in fragments shorter than    1642 bp
  85% of sequence in fragments shorter than    1898 bp
  90% of sequence in fragments shorter than    2251 bp
  95% of sequence in fragments shorter than    2901 bp

# EcoRI.HphI (GAATTC+GGTGA) double digest, 6854 thousand fragments (847401+6007336 sites), 434 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     294 bp
  20% of sequence in fragments shorter than     351 bp
  25% of sequence in fragments shorter than     415 bp
  30% of sequence in fragments shorter than     480 bp
  35% of sequence in fragments shorter than     543 bp
  40% of sequence in fragments shorter than     605 bp
  45% of sequence in fragments shorter than     675 bp
  50% of sequence in fragments shorter than     745 bp
  55% of sequence in fragments shorter than     824 bp
  60% of sequence in fragments shorter than     910 bp
  65% of sequence in fragments shorter than    1008 bp
  70% of sequence in fragments shorter than    1117 bp
  75% of sequence in fragments shorter than    1242 bp
  80% of sequence in fragments shorter than    1386 bp
  85% of sequence in fragments shorter than    1578 bp
  90% of sequence in fragments shorter than    1855 bp
  95% of sequence in fragments shorter than    2304 bp

# AflII.BthCI (CTTAAG+GCNGC) double digest, 6852 thousand fragments (643284+6209929 sites), 434 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     243 bp
  15% of sequence in fragments shorter than     326 bp
  20% of sequence in fragments shorter than     411 bp
  25% of sequence in fragments shorter than     498 bp
  30% of sequence in fragments shorter than     581 bp
  35% of sequence in fragments shorter than     672 bp
  40% of sequence in fragments shorter than     766 bp
  45% of sequence in fragments shorter than     862 bp
  50% of sequence in fragments shorter than     967 bp
  55% of sequence in fragments shorter than    1081 bp
  60% of sequence in fragments shorter than    1205 bp
  65% of sequence in fragments shorter than    1344 bp
  70% of sequence in fragments shorter than    1501 bp
  75% of sequence in fragments shorter than    1688 bp
  80% of sequence in fragments shorter than    1913 bp
  85% of sequence in fragments shorter than    2193 bp
  90% of sequence in fragments shorter than    2596 bp
  95% of sequence in fragments shorter than    3277 bp

# FokI.PsiI (GGATG+TTATAA) double digest, 6848 thousand fragments (5201116+1647912 sites), 435 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     427 bp
  30% of sequence in fragments shorter than     492 bp
  35% of sequence in fragments shorter than     556 bp
  40% of sequence in fragments shorter than     622 bp
  45% of sequence in fragments shorter than     692 bp
  50% of sequence in fragments shorter than     766 bp
  55% of sequence in fragments shorter than     846 bp
  60% of sequence in fragments shorter than     928 bp
  65% of sequence in fragments shorter than    1021 bp
  70% of sequence in fragments shorter than    1122 bp
  75% of sequence in fragments shorter than    1240 bp
  80% of sequence in fragments shorter than    1383 bp
  85% of sequence in fragments shorter than    1563 bp
  90% of sequence in fragments shorter than    1817 bp
  95% of sequence in fragments shorter than    2279 bp

# ApaI.ApoI (GGGCCC+RAATTY) double digest, 6842 thousand fragments (460339+6382433 sites), 435 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     293 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     426 bp
  30% of sequence in fragments shorter than     496 bp
  35% of sequence in fragments shorter than     567 bp
  40% of sequence in fragments shorter than     640 bp
  45% of sequence in fragments shorter than     715 bp
  50% of sequence in fragments shorter than     799 bp
  55% of sequence in fragments shorter than     886 bp
  60% of sequence in fragments shorter than     980 bp
  65% of sequence in fragments shorter than    1087 bp
  70% of sequence in fragments shorter than    1211 bp
  75% of sequence in fragments shorter than    1346 bp
  80% of sequence in fragments shorter than    1515 bp
  85% of sequence in fragments shorter than    1738 bp
  90% of sequence in fragments shorter than    2061 bp
  95% of sequence in fragments shorter than    2629 bp

# AccI.BsrI (GTMKAC+ACTGG) double digest, 6841 thousand fragments (1101063+5741310 sites), 435 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     425 bp
  30% of sequence in fragments shorter than     487 bp
  35% of sequence in fragments shorter than     553 bp
  40% of sequence in fragments shorter than     621 bp
  45% of sequence in fragments shorter than     690 bp
  50% of sequence in fragments shorter than     758 bp
  55% of sequence in fragments shorter than     838 bp
  60% of sequence in fragments shorter than     923 bp
  65% of sequence in fragments shorter than    1020 bp
  70% of sequence in fragments shorter than    1127 bp
  75% of sequence in fragments shorter than    1252 bp
  80% of sequence in fragments shorter than    1401 bp
  85% of sequence in fragments shorter than    1591 bp
  90% of sequence in fragments shorter than    1858 bp
  95% of sequence in fragments shorter than    2319 bp

# BbvI.HgiCI (GCAGC+GGYRCC) double digest, 6840 thousand fragments (5290051+1550877 sites), 435 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     231 bp
  15% of sequence in fragments shorter than     307 bp
  20% of sequence in fragments shorter than     386 bp
  25% of sequence in fragments shorter than     466 bp
  30% of sequence in fragments shorter than     550 bp
  35% of sequence in fragments shorter than     633 bp
  40% of sequence in fragments shorter than     723 bp
  45% of sequence in fragments shorter than     821 bp
  50% of sequence in fragments shorter than     917 bp
  55% of sequence in fragments shorter than    1027 bp
  60% of sequence in fragments shorter than    1147 bp
  65% of sequence in fragments shorter than    1283 bp
  70% of sequence in fragments shorter than    1442 bp
  75% of sequence in fragments shorter than    1621 bp
  80% of sequence in fragments shorter than    1849 bp
  85% of sequence in fragments shorter than    2138 bp
  90% of sequence in fragments shorter than    2563 bp
  95% of sequence in fragments shorter than    3282 bp

# BthCI.HaeII (GCNGC+RGCGCY) double digest, 6839 thousand fragments (6209929+630101 sites), 435 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     245 bp
  15% of sequence in fragments shorter than     333 bp
  20% of sequence in fragments shorter than     418 bp
  25% of sequence in fragments shorter than     513 bp
  30% of sequence in fragments shorter than     608 bp
  35% of sequence in fragments shorter than     703 bp
  40% of sequence in fragments shorter than     810 bp
  45% of sequence in fragments shorter than     914 bp
  50% of sequence in fragments shorter than    1033 bp
  55% of sequence in fragments shorter than    1161 bp
  60% of sequence in fragments shorter than    1304 bp
  65% of sequence in fragments shorter than    1464 bp
  70% of sequence in fragments shorter than    1640 bp
  75% of sequence in fragments shorter than    1850 bp
  80% of sequence in fragments shorter than    2095 bp
  85% of sequence in fragments shorter than    2426 bp
  90% of sequence in fragments shorter than    2892 bp
  95% of sequence in fragments shorter than    3693 bp

# BbvI.BsmI (GCAGC+GAATGC) double digest, 6838 thousand fragments (5290051+1549350 sites), 435 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     382 bp
  25% of sequence in fragments shorter than     465 bp
  30% of sequence in fragments shorter than     549 bp
  35% of sequence in fragments shorter than     636 bp
  40% of sequence in fragments shorter than     727 bp
  45% of sequence in fragments shorter than     822 bp
  50% of sequence in fragments shorter than     922 bp
  55% of sequence in fragments shorter than    1029 bp
  60% of sequence in fragments shorter than    1142 bp
  65% of sequence in fragments shorter than    1275 bp
  70% of sequence in fragments shorter than    1421 bp
  75% of sequence in fragments shorter than    1596 bp
  80% of sequence in fragments shorter than    1805 bp
  85% of sequence in fragments shorter than    2067 bp
  90% of sequence in fragments shorter than    2434 bp
  95% of sequence in fragments shorter than    3062 bp

# ApoI.EcoRV (RAATTY+GATATC) double digest, 6828 thousand fragments (6382433+446473 sites), 436 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     290 bp
  20% of sequence in fragments shorter than     355 bp
  25% of sequence in fragments shorter than     425 bp
  30% of sequence in fragments shorter than     497 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     648 bp
  45% of sequence in fragments shorter than     728 bp
  50% of sequence in fragments shorter than     818 bp
  55% of sequence in fragments shorter than     911 bp
  60% of sequence in fragments shorter than    1018 bp
  65% of sequence in fragments shorter than    1139 bp
  70% of sequence in fragments shorter than    1281 bp
  75% of sequence in fragments shorter than    1436 bp
  80% of sequence in fragments shorter than    1651 bp
  85% of sequence in fragments shorter than    1938 bp
  90% of sequence in fragments shorter than    2382 bp
  95% of sequence in fragments shorter than    3283 bp

# BsrI.PpuMI (ACTGG+RGGWCCY) double digest, 6825 thousand fragments (5741310+1085138 sites), 436 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     296 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     428 bp
  30% of sequence in fragments shorter than     493 bp
  35% of sequence in fragments shorter than     563 bp
  40% of sequence in fragments shorter than     636 bp
  45% of sequence in fragments shorter than     708 bp
  50% of sequence in fragments shorter than     787 bp
  55% of sequence in fragments shorter than     873 bp
  60% of sequence in fragments shorter than     969 bp
  65% of sequence in fragments shorter than    1074 bp
  70% of sequence in fragments shorter than    1195 bp
  75% of sequence in fragments shorter than    1338 bp
  80% of sequence in fragments shorter than    1506 bp
  85% of sequence in fragments shorter than    1726 bp
  90% of sequence in fragments shorter than    2039 bp
  95% of sequence in fragments shorter than    2604 bp

# BsrI.PvuII (ACTGG+CAGCTG) double digest, 6825 thousand fragments (5741310+1084260 sites), 436 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     223 bp
  15% of sequence in fragments shorter than     294 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     428 bp
  30% of sequence in fragments shorter than     492 bp
  35% of sequence in fragments shorter than     560 bp
  40% of sequence in fragments shorter than     631 bp
  45% of sequence in fragments shorter than     702 bp
  50% of sequence in fragments shorter than     776 bp
  55% of sequence in fragments shorter than     860 bp
  60% of sequence in fragments shorter than     951 bp
  65% of sequence in fragments shorter than    1052 bp
  70% of sequence in fragments shorter than    1170 bp
  75% of sequence in fragments shorter than    1307 bp
  80% of sequence in fragments shorter than    1469 bp
  85% of sequence in fragments shorter than    1683 bp
  90% of sequence in fragments shorter than    1983 bp
  95% of sequence in fragments shorter than    2523 bp

# BfiI.CviQI (ACTGGG+GTAC) double digest, 6821 thousand fragments (1706594+5115078 sites), 436 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     306 bp
  20% of sequence in fragments shorter than     371 bp
  25% of sequence in fragments shorter than     435 bp
  30% of sequence in fragments shorter than     496 bp
  35% of sequence in fragments shorter than     558 bp
  40% of sequence in fragments shorter than     626 bp
  45% of sequence in fragments shorter than     693 bp
  50% of sequence in fragments shorter than     766 bp
  55% of sequence in fragments shorter than     844 bp
  60% of sequence in fragments shorter than     922 bp
  65% of sequence in fragments shorter than    1016 bp
  70% of sequence in fragments shorter than    1121 bp
  75% of sequence in fragments shorter than    1238 bp
  80% of sequence in fragments shorter than    1381 bp
  85% of sequence in fragments shorter than    1568 bp
  90% of sequence in fragments shorter than    1827 bp
  95% of sequence in fragments shorter than    2281 bp

# AflII.BccI (CTTAAG+CCATC) double digest, 6813 thousand fragments (643284+6170927 sites), 437 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     297 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     424 bp
  30% of sequence in fragments shorter than     488 bp
  35% of sequence in fragments shorter than     556 bp
  40% of sequence in fragments shorter than     626 bp
  45% of sequence in fragments shorter than     699 bp
  50% of sequence in fragments shorter than     776 bp
  55% of sequence in fragments shorter than     859 bp
  60% of sequence in fragments shorter than     950 bp
  65% of sequence in fragments shorter than    1053 bp
  70% of sequence in fragments shorter than    1163 bp
  75% of sequence in fragments shorter than    1296 bp
  80% of sequence in fragments shorter than    1463 bp
  85% of sequence in fragments shorter than    1677 bp
  90% of sequence in fragments shorter than    1986 bp
  95% of sequence in fragments shorter than    2569 bp

# BthCI.SacI (GCNGC+GAGCTC) double digest, 6812 thousand fragments (6209929+603323 sites), 437 bp average fragment length
   5% of sequence in fragments shorter than     143 bp
  10% of sequence in fragments shorter than     242 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     416 bp
  25% of sequence in fragments shorter than     508 bp
  30% of sequence in fragments shorter than     601 bp
  35% of sequence in fragments shorter than     695 bp
  40% of sequence in fragments shorter than     800 bp
  45% of sequence in fragments shorter than     900 bp
  50% of sequence in fragments shorter than    1016 bp
  55% of sequence in fragments shorter than    1136 bp
  60% of sequence in fragments shorter than    1275 bp
  65% of sequence in fragments shorter than    1427 bp
  70% of sequence in fragments shorter than    1606 bp
  75% of sequence in fragments shorter than    1802 bp
  80% of sequence in fragments shorter than    2042 bp
  85% of sequence in fragments shorter than    2360 bp
  90% of sequence in fragments shorter than    2805 bp
  95% of sequence in fragments shorter than    3575 bp

# AvrII.BthCI (CCTAGG+GCNGC) double digest, 6804 thousand fragments (594956+6209929 sites), 437 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     244 bp
  15% of sequence in fragments shorter than     327 bp
  20% of sequence in fragments shorter than     415 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     599 bp
  35% of sequence in fragments shorter than     686 bp
  40% of sequence in fragments shorter than     786 bp
  45% of sequence in fragments shorter than     886 bp
  50% of sequence in fragments shorter than     999 bp
  55% of sequence in fragments shorter than    1121 bp
  60% of sequence in fragments shorter than    1255 bp
  65% of sequence in fragments shorter than    1401 bp
  70% of sequence in fragments shorter than    1577 bp
  75% of sequence in fragments shorter than    1777 bp
  80% of sequence in fragments shorter than    2028 bp
  85% of sequence in fragments shorter than    2329 bp
  90% of sequence in fragments shorter than    2765 bp
  95% of sequence in fragments shorter than    3513 bp

# EsaBC3I.FokI (TCGA+GGATG) double digest, 6803 thousand fragments (1603346+5201116 sites), 437 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     369 bp
  25% of sequence in fragments shorter than     434 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     567 bp
  40% of sequence in fragments shorter than     637 bp
  45% of sequence in fragments shorter than     711 bp
  50% of sequence in fragments shorter than     788 bp
  55% of sequence in fragments shorter than     873 bp
  60% of sequence in fragments shorter than     960 bp
  65% of sequence in fragments shorter than    1060 bp
  70% of sequence in fragments shorter than    1172 bp
  75% of sequence in fragments shorter than    1307 bp
  80% of sequence in fragments shorter than    1462 bp
  85% of sequence in fragments shorter than    1668 bp
  90% of sequence in fragments shorter than    1956 bp
  95% of sequence in fragments shorter than    2458 bp

# AclI.BsmAI (AACGTT+GTCTC) double digest, 6803 thousand fragments (172706+6631585 sites), 437 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     372 bp
  25% of sequence in fragments shorter than     446 bp
  30% of sequence in fragments shorter than     520 bp
  35% of sequence in fragments shorter than     592 bp
  40% of sequence in fragments shorter than     671 bp
  45% of sequence in fragments shorter than     755 bp
  50% of sequence in fragments shorter than     846 bp
  55% of sequence in fragments shorter than     943 bp
  60% of sequence in fragments shorter than    1049 bp
  65% of sequence in fragments shorter than    1169 bp
  70% of sequence in fragments shorter than    1307 bp
  75% of sequence in fragments shorter than    1470 bp
  80% of sequence in fragments shorter than    1664 bp
  85% of sequence in fragments shorter than    1922 bp
  90% of sequence in fragments shorter than    2275 bp
  95% of sequence in fragments shorter than    2924 bp

# BccI.HaeII (CCATC+RGCGCY) double digest, 6800 thousand fragments (6170927+630101 sites), 438 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     302 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     432 bp
  30% of sequence in fragments shorter than     499 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     643 bp
  45% of sequence in fragments shorter than     716 bp
  50% of sequence in fragments shorter than     799 bp
  55% of sequence in fragments shorter than     887 bp
  60% of sequence in fragments shorter than     986 bp
  65% of sequence in fragments shorter than    1087 bp
  70% of sequence in fragments shorter than    1210 bp
  75% of sequence in fragments shorter than    1353 bp
  80% of sequence in fragments shorter than    1527 bp
  85% of sequence in fragments shorter than    1749 bp
  90% of sequence in fragments shorter than    2066 bp
  95% of sequence in fragments shorter than    2614 bp

# BsmAI.PacI (GTCTC+TTAATTAA) double digest, 6790 thousand fragments (6631585+159455 sites), 438 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     296 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     448 bp
  30% of sequence in fragments shorter than     522 bp
  35% of sequence in fragments shorter than     595 bp
  40% of sequence in fragments shorter than     674 bp
  45% of sequence in fragments shorter than     759 bp
  50% of sequence in fragments shorter than     850 bp
  55% of sequence in fragments shorter than     948 bp
  60% of sequence in fragments shorter than    1056 bp
  65% of sequence in fragments shorter than    1177 bp
  70% of sequence in fragments shorter than    1317 bp
  75% of sequence in fragments shorter than    1483 bp
  80% of sequence in fragments shorter than    1679 bp
  85% of sequence in fragments shorter than    1939 bp
  90% of sequence in fragments shorter than    2300 bp
  95% of sequence in fragments shorter than    2972 bp

# BsmAI.Hpy99I (GTCTC+CGWCG) double digest, 6789 thousand fragments (6631585+157917 sites), 438 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     210 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     448 bp
  30% of sequence in fragments shorter than     522 bp
  35% of sequence in fragments shorter than     597 bp
  40% of sequence in fragments shorter than     677 bp
  45% of sequence in fragments shorter than     764 bp
  50% of sequence in fragments shorter than     858 bp
  55% of sequence in fragments shorter than     958 bp
  60% of sequence in fragments shorter than    1069 bp
  65% of sequence in fragments shorter than    1194 bp
  70% of sequence in fragments shorter than    1339 bp
  75% of sequence in fragments shorter than    1508 bp
  80% of sequence in fragments shorter than    1715 bp
  85% of sequence in fragments shorter than    1984 bp
  90% of sequence in fragments shorter than    2357 bp
  95% of sequence in fragments shorter than    3051 bp

# ApoI.SpeI (RAATTY+ACTAGT) double digest, 6782 thousand fragments (6382433+400286 sites), 439 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     292 bp
  20% of sequence in fragments shorter than     359 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     501 bp
  35% of sequence in fragments shorter than     575 bp
  40% of sequence in fragments shorter than     650 bp
  45% of sequence in fragments shorter than     729 bp
  50% of sequence in fragments shorter than     819 bp
  55% of sequence in fragments shorter than     910 bp
  60% of sequence in fragments shorter than    1019 bp
  65% of sequence in fragments shorter than    1141 bp
  70% of sequence in fragments shorter than    1284 bp
  75% of sequence in fragments shorter than    1443 bp
  80% of sequence in fragments shorter than    1661 bp
  85% of sequence in fragments shorter than    1952 bp
  90% of sequence in fragments shorter than    2407 bp
  95% of sequence in fragments shorter than    3354 bp

# BglII.HphI (AGATCT+GGTGA) double digest, 6781 thousand fragments (774732+6007336 sites), 439 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     296 bp
  20% of sequence in fragments shorter than     357 bp
  25% of sequence in fragments shorter than     421 bp
  30% of sequence in fragments shorter than     485 bp
  35% of sequence in fragments shorter than     549 bp
  40% of sequence in fragments shorter than     615 bp
  45% of sequence in fragments shorter than     686 bp
  50% of sequence in fragments shorter than     760 bp
  55% of sequence in fragments shorter than     838 bp
  60% of sequence in fragments shorter than     926 bp
  65% of sequence in fragments shorter than    1026 bp
  70% of sequence in fragments shorter than    1133 bp
  75% of sequence in fragments shorter than    1264 bp
  80% of sequence in fragments shorter than    1414 bp
  85% of sequence in fragments shorter than    1616 bp
  90% of sequence in fragments shorter than    1909 bp
  95% of sequence in fragments shorter than    2405 bp

# BthCI.HgaI (GCNGC+GACGC) double digest, 6781 thousand fragments (6209929+571889 sites), 439 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     336 bp
  20% of sequence in fragments shorter than     427 bp
  25% of sequence in fragments shorter than     522 bp
  30% of sequence in fragments shorter than     619 bp
  35% of sequence in fragments shorter than     719 bp
  40% of sequence in fragments shorter than     825 bp
  45% of sequence in fragments shorter than     932 bp
  50% of sequence in fragments shorter than    1049 bp
  55% of sequence in fragments shorter than    1180 bp
  60% of sequence in fragments shorter than    1322 bp
  65% of sequence in fragments shorter than    1481 bp
  70% of sequence in fragments shorter than    1655 bp
  75% of sequence in fragments shorter than    1870 bp
  80% of sequence in fragments shorter than    2118 bp
  85% of sequence in fragments shorter than    2450 bp
  90% of sequence in fragments shorter than    2915 bp
  95% of sequence in fragments shorter than    3718 bp

# BthCI.EciI (GCNGC+GGCGGA) double digest, 6780 thousand fragments (6209929+571273 sites), 439 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     243 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     415 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     596 bp
  35% of sequence in fragments shorter than     690 bp
  40% of sequence in fragments shorter than     792 bp
  45% of sequence in fragments shorter than     893 bp
  50% of sequence in fragments shorter than    1008 bp
  55% of sequence in fragments shorter than    1129 bp
  60% of sequence in fragments shorter than    1267 bp
  65% of sequence in fragments shorter than    1420 bp
  70% of sequence in fragments shorter than    1599 bp
  75% of sequence in fragments shorter than    1796 bp
  80% of sequence in fragments shorter than    2050 bp
  85% of sequence in fragments shorter than    2363 bp
  90% of sequence in fragments shorter than    2817 bp
  95% of sequence in fragments shorter than    3612 bp

# AflIII.BbvI (ACRYGT+GCAGC) double digest, 6774 thousand fragments (1485394+5290051 sites), 439 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     314 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     470 bp
  30% of sequence in fragments shorter than     550 bp
  35% of sequence in fragments shorter than     634 bp
  40% of sequence in fragments shorter than     720 bp
  45% of sequence in fragments shorter than     811 bp
  50% of sequence in fragments shorter than     904 bp
  55% of sequence in fragments shorter than    1009 bp
  60% of sequence in fragments shorter than    1123 bp
  65% of sequence in fragments shorter than    1249 bp
  70% of sequence in fragments shorter than    1393 bp
  75% of sequence in fragments shorter than    1565 bp
  80% of sequence in fragments shorter than    1760 bp
  85% of sequence in fragments shorter than    2017 bp
  90% of sequence in fragments shorter than    2370 bp
  95% of sequence in fragments shorter than    3008 bp

# BccI.SacI (CCATC+GAGCTC) double digest, 6773 thousand fragments (6170927+603323 sites), 439 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     430 bp
  30% of sequence in fragments shorter than     496 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     637 bp
  45% of sequence in fragments shorter than     712 bp
  50% of sequence in fragments shorter than     792 bp
  55% of sequence in fragments shorter than     879 bp
  60% of sequence in fragments shorter than     976 bp
  65% of sequence in fragments shorter than    1079 bp
  70% of sequence in fragments shorter than    1200 bp
  75% of sequence in fragments shorter than    1341 bp
  80% of sequence in fragments shorter than    1518 bp
  85% of sequence in fragments shorter than    1744 bp
  90% of sequence in fragments shorter than    2077 bp
  95% of sequence in fragments shorter than    2691 bp

# BthCI.MfeI (GCNGC+CAATTG) double digest, 6773 thousand fragments (6209929+564303 sites), 439 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     416 bp
  25% of sequence in fragments shorter than     504 bp
  30% of sequence in fragments shorter than     593 bp
  35% of sequence in fragments shorter than     680 bp
  40% of sequence in fragments shorter than     777 bp
  45% of sequence in fragments shorter than     872 bp
  50% of sequence in fragments shorter than     979 bp
  55% of sequence in fragments shorter than    1090 bp
  60% of sequence in fragments shorter than    1218 bp
  65% of sequence in fragments shorter than    1358 bp
  70% of sequence in fragments shorter than    1517 bp
  75% of sequence in fragments shorter than    1704 bp
  80% of sequence in fragments shorter than    1929 bp
  85% of sequence in fragments shorter than    2208 bp
  90% of sequence in fragments shorter than    2609 bp
  95% of sequence in fragments shorter than    3273 bp

# ApoI.HpaI (RAATTY+GTTAAC) double digest, 6771 thousand fragments (6382433+389418 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     225 bp
  15% of sequence in fragments shorter than     293 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     501 bp
  35% of sequence in fragments shorter than     576 bp
  40% of sequence in fragments shorter than     653 bp
  45% of sequence in fragments shorter than     733 bp
  50% of sequence in fragments shorter than     824 bp
  55% of sequence in fragments shorter than     918 bp
  60% of sequence in fragments shorter than    1025 bp
  65% of sequence in fragments shorter than    1147 bp
  70% of sequence in fragments shorter than    1290 bp
  75% of sequence in fragments shorter than    1446 bp
  80% of sequence in fragments shorter than    1662 bp
  85% of sequence in fragments shorter than    1951 bp
  90% of sequence in fragments shorter than    2398 bp
  95% of sequence in fragments shorter than    3335 bp

# AvaII.Tsp45I (GGWCC+GTSAC) double digest, 6768 thousand fragments (2747690+4021761 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     569 bp
  40% of sequence in fragments shorter than     645 bp
  45% of sequence in fragments shorter than     720 bp
  50% of sequence in fragments shorter than     806 bp
  55% of sequence in fragments shorter than     896 bp
  60% of sequence in fragments shorter than     991 bp
  65% of sequence in fragments shorter than    1101 bp
  70% of sequence in fragments shorter than    1231 bp
  75% of sequence in fragments shorter than    1381 bp
  80% of sequence in fragments shorter than    1568 bp
  85% of sequence in fragments shorter than    1803 bp
  90% of sequence in fragments shorter than    2127 bp
  95% of sequence in fragments shorter than    2697 bp

# BscAI.StyI (GCATC+CCWWGG) double digest, 6766 thousand fragments (3652926+3114107 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     496 bp
  35% of sequence in fragments shorter than     565 bp
  40% of sequence in fragments shorter than     637 bp
  45% of sequence in fragments shorter than     713 bp
  50% of sequence in fragments shorter than     788 bp
  55% of sequence in fragments shorter than     874 bp
  60% of sequence in fragments shorter than     970 bp
  65% of sequence in fragments shorter than    1078 bp
  70% of sequence in fragments shorter than    1199 bp
  75% of sequence in fragments shorter than    1339 bp
  80% of sequence in fragments shorter than    1514 bp
  85% of sequence in fragments shorter than    1727 bp
  90% of sequence in fragments shorter than    2042 bp
  95% of sequence in fragments shorter than    2622 bp

# HphI.NcoI (GGTGA+CCATGG) double digest, 6766 thousand fragments (6007336+759594 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     296 bp
  20% of sequence in fragments shorter than     358 bp
  25% of sequence in fragments shorter than     424 bp
  30% of sequence in fragments shorter than     489 bp
  35% of sequence in fragments shorter than     555 bp
  40% of sequence in fragments shorter than     623 bp
  45% of sequence in fragments shorter than     694 bp
  50% of sequence in fragments shorter than     772 bp
  55% of sequence in fragments shorter than     854 bp
  60% of sequence in fragments shorter than     945 bp
  65% of sequence in fragments shorter than    1048 bp
  70% of sequence in fragments shorter than    1166 bp
  75% of sequence in fragments shorter than    1303 bp
  80% of sequence in fragments shorter than    1454 bp
  85% of sequence in fragments shorter than    1665 bp
  90% of sequence in fragments shorter than    1959 bp
  95% of sequence in fragments shorter than    2469 bp

# AvrII.BccI (CCTAGG+CCATC) double digest, 6765 thousand fragments (594956+6170927 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     431 bp
  30% of sequence in fragments shorter than     496 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     639 bp
  45% of sequence in fragments shorter than     714 bp
  50% of sequence in fragments shorter than     795 bp
  55% of sequence in fragments shorter than     882 bp
  60% of sequence in fragments shorter than     978 bp
  65% of sequence in fragments shorter than    1081 bp
  70% of sequence in fragments shorter than    1202 bp
  75% of sequence in fragments shorter than    1343 bp
  80% of sequence in fragments shorter than    1521 bp
  85% of sequence in fragments shorter than    1749 bp
  90% of sequence in fragments shorter than    2085 bp
  95% of sequence in fragments shorter than    2708 bp

# BsmAI.SnaBI (GTCTC+TACGTA) double digest, 6763 thousand fragments (6631585+132299 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     297 bp
  20% of sequence in fragments shorter than     374 bp
  25% of sequence in fragments shorter than     449 bp
  30% of sequence in fragments shorter than     523 bp
  35% of sequence in fragments shorter than     597 bp
  40% of sequence in fragments shorter than     676 bp
  45% of sequence in fragments shorter than     762 bp
  50% of sequence in fragments shorter than     854 bp
  55% of sequence in fragments shorter than     953 bp
  60% of sequence in fragments shorter than    1061 bp
  65% of sequence in fragments shorter than    1184 bp
  70% of sequence in fragments shorter than    1324 bp
  75% of sequence in fragments shorter than    1487 bp
  80% of sequence in fragments shorter than    1684 bp
  85% of sequence in fragments shorter than    1942 bp
  90% of sequence in fragments shorter than    2301 bp
  95% of sequence in fragments shorter than    2962 bp

# CviQI.PsiI (GTAC+TTATAA) double digest, 6762 thousand fragments (5115078+1647912 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     371 bp
  25% of sequence in fragments shorter than     437 bp
  30% of sequence in fragments shorter than     501 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     637 bp
  45% of sequence in fragments shorter than     708 bp
  50% of sequence in fragments shorter than     783 bp
  55% of sequence in fragments shorter than     866 bp
  60% of sequence in fragments shorter than     955 bp
  65% of sequence in fragments shorter than    1055 bp
  70% of sequence in fragments shorter than    1174 bp
  75% of sequence in fragments shorter than    1303 bp
  80% of sequence in fragments shorter than    1459 bp
  85% of sequence in fragments shorter than    1676 bp
  90% of sequence in fragments shorter than    1992 bp
  95% of sequence in fragments shorter than    2571 bp

# BthCI.SphI (GCNGC+GCATGC) double digest, 6760 thousand fragments (6209929+550952 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     333 bp
  20% of sequence in fragments shorter than     420 bp
  25% of sequence in fragments shorter than     512 bp
  30% of sequence in fragments shorter than     604 bp
  35% of sequence in fragments shorter than     697 bp
  40% of sequence in fragments shorter than     801 bp
  45% of sequence in fragments shorter than     902 bp
  50% of sequence in fragments shorter than    1016 bp
  55% of sequence in fragments shorter than    1136 bp
  60% of sequence in fragments shorter than    1272 bp
  65% of sequence in fragments shorter than    1423 bp
  70% of sequence in fragments shorter than    1599 bp
  75% of sequence in fragments shorter than    1793 bp
  80% of sequence in fragments shorter than    2040 bp
  85% of sequence in fragments shorter than    2346 bp
  90% of sequence in fragments shorter than    2789 bp
  95% of sequence in fragments shorter than    3548 bp

# ApoI.SapI (RAATTY+GCTCTTC) double digest, 6759 thousand fragments (6382433+377161 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     430 bp
  30% of sequence in fragments shorter than     502 bp
  35% of sequence in fragments shorter than     575 bp
  40% of sequence in fragments shorter than     650 bp
  45% of sequence in fragments shorter than     729 bp
  50% of sequence in fragments shorter than     818 bp
  55% of sequence in fragments shorter than     909 bp
  60% of sequence in fragments shorter than    1014 bp
  65% of sequence in fragments shorter than    1131 bp
  70% of sequence in fragments shorter than    1267 bp
  75% of sequence in fragments shorter than    1414 bp
  80% of sequence in fragments shorter than    1614 bp
  85% of sequence in fragments shorter than    1881 bp
  90% of sequence in fragments shorter than    2274 bp
  95% of sequence in fragments shorter than    3058 bp

# ApoI.SmaI (RAATTY+CCCGGG) double digest, 6758 thousand fragments (6382433+376940 sites), 440 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     294 bp
  20% of sequence in fragments shorter than     360 bp
  25% of sequence in fragments shorter than     428 bp
  30% of sequence in fragments shorter than     498 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     643 bp
  45% of sequence in fragments shorter than     720 bp
  50% of sequence in fragments shorter than     806 bp
  55% of sequence in fragments shorter than     893 bp
  60% of sequence in fragments shorter than     991 bp
  65% of sequence in fragments shorter than    1102 bp
  70% of sequence in fragments shorter than    1230 bp
  75% of sequence in fragments shorter than    1372 bp
  80% of sequence in fragments shorter than    1549 bp
  85% of sequence in fragments shorter than    1787 bp
  90% of sequence in fragments shorter than    2125 bp
  95% of sequence in fragments shorter than    2766 bp

# BscAI.NspI (GCATC+RCATGY) double digest, 6756 thousand fragments (3652926+3104359 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     302 bp
  20% of sequence in fragments shorter than     369 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     499 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     634 bp
  45% of sequence in fragments shorter than     703 bp
  50% of sequence in fragments shorter than     778 bp
  55% of sequence in fragments shorter than     858 bp
  60% of sequence in fragments shorter than     947 bp
  65% of sequence in fragments shorter than    1041 bp
  70% of sequence in fragments shorter than    1153 bp
  75% of sequence in fragments shorter than    1281 bp
  80% of sequence in fragments shorter than    1431 bp
  85% of sequence in fragments shorter than    1625 bp
  90% of sequence in fragments shorter than    1905 bp
  95% of sequence in fragments shorter than    2400 bp

# BsmAI.NaeI (GTCTC+GCCGGC) double digest, 6756 thousand fragments (6631585+125692 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     211 bp
  15% of sequence in fragments shorter than     297 bp
  20% of sequence in fragments shorter than     374 bp
  25% of sequence in fragments shorter than     450 bp
  30% of sequence in fragments shorter than     525 bp
  35% of sequence in fragments shorter than     600 bp
  40% of sequence in fragments shorter than     681 bp
  45% of sequence in fragments shorter than     768 bp
  50% of sequence in fragments shorter than     862 bp
  55% of sequence in fragments shorter than     963 bp
  60% of sequence in fragments shorter than    1074 bp
  65% of sequence in fragments shorter than    1201 bp
  70% of sequence in fragments shorter than    1346 bp
  75% of sequence in fragments shorter than    1517 bp
  80% of sequence in fragments shorter than    1726 bp
  85% of sequence in fragments shorter than    1998 bp
  90% of sequence in fragments shorter than    2378 bp
  95% of sequence in fragments shorter than    3078 bp

# BthCI.ScaI (GCNGC+AGTACT) double digest, 6753 thousand fragments (6209929+543793 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     417 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     595 bp
  35% of sequence in fragments shorter than     687 bp
  40% of sequence in fragments shorter than     783 bp
  45% of sequence in fragments shorter than     879 bp
  50% of sequence in fragments shorter than     988 bp
  55% of sequence in fragments shorter than    1100 bp
  60% of sequence in fragments shorter than    1228 bp
  65% of sequence in fragments shorter than    1370 bp
  70% of sequence in fragments shorter than    1531 bp
  75% of sequence in fragments shorter than    1711 bp
  80% of sequence in fragments shorter than    1937 bp
  85% of sequence in fragments shorter than    2215 bp
  90% of sequence in fragments shorter than    2625 bp
  95% of sequence in fragments shorter than    3310 bp

# BsmAI.XhoI (GTCTC+CTCGAG) double digest, 6752 thousand fragments (6631585+121308 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     212 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     452 bp
  30% of sequence in fragments shorter than     526 bp
  35% of sequence in fragments shorter than     601 bp
  40% of sequence in fragments shorter than     682 bp
  45% of sequence in fragments shorter than     768 bp
  50% of sequence in fragments shorter than     861 bp
  55% of sequence in fragments shorter than     961 bp
  60% of sequence in fragments shorter than    1071 bp
  65% of sequence in fragments shorter than    1197 bp
  70% of sequence in fragments shorter than    1341 bp
  75% of sequence in fragments shorter than    1511 bp
  80% of sequence in fragments shorter than    1721 bp
  85% of sequence in fragments shorter than    1991 bp
  90% of sequence in fragments shorter than    2368 bp
  95% of sequence in fragments shorter than    3062 bp

# FokI.HgiCI (GGATG+GGYRCC) double digest, 6751 thousand fragments (5201116+1550877 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     367 bp
  25% of sequence in fragments shorter than     438 bp
  30% of sequence in fragments shorter than     509 bp
  35% of sequence in fragments shorter than     581 bp
  40% of sequence in fragments shorter than     656 bp
  45% of sequence in fragments shorter than     734 bp
  50% of sequence in fragments shorter than     816 bp
  55% of sequence in fragments shorter than     906 bp
  60% of sequence in fragments shorter than    1006 bp
  65% of sequence in fragments shorter than    1114 bp
  70% of sequence in fragments shorter than    1238 bp
  75% of sequence in fragments shorter than    1379 bp
  80% of sequence in fragments shorter than    1555 bp
  85% of sequence in fragments shorter than    1784 bp
  90% of sequence in fragments shorter than    2111 bp
  95% of sequence in fragments shorter than    2710 bp

# BsmI.FokI (GAATGC+GGATG) double digest, 6749 thousand fragments (1549350+5201116 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     369 bp
  25% of sequence in fragments shorter than     441 bp
  30% of sequence in fragments shorter than     513 bp
  35% of sequence in fragments shorter than     586 bp
  40% of sequence in fragments shorter than     661 bp
  45% of sequence in fragments shorter than     739 bp
  50% of sequence in fragments shorter than     822 bp
  55% of sequence in fragments shorter than     910 bp
  60% of sequence in fragments shorter than     998 bp
  65% of sequence in fragments shorter than    1102 bp
  70% of sequence in fragments shorter than    1220 bp
  75% of sequence in fragments shorter than    1358 bp
  80% of sequence in fragments shorter than    1518 bp
  85% of sequence in fragments shorter than    1727 bp
  90% of sequence in fragments shorter than    2021 bp
  95% of sequence in fragments shorter than    2515 bp

# SmlI.Tsp45I (CTYRAG+GTSAC) double digest, 6749 thousand fragments (2727867+4021761 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     358 bp
  25% of sequence in fragments shorter than     424 bp
  30% of sequence in fragments shorter than     489 bp
  35% of sequence in fragments shorter than     554 bp
  40% of sequence in fragments shorter than     622 bp
  45% of sequence in fragments shorter than     691 bp
  50% of sequence in fragments shorter than     767 bp
  55% of sequence in fragments shorter than     850 bp
  60% of sequence in fragments shorter than     938 bp
  65% of sequence in fragments shorter than    1038 bp
  70% of sequence in fragments shorter than    1152 bp
  75% of sequence in fragments shorter than    1286 bp
  80% of sequence in fragments shorter than    1446 bp
  85% of sequence in fragments shorter than    1662 bp
  90% of sequence in fragments shorter than    1949 bp
  95% of sequence in fragments shorter than    2436 bp

# ApoI.BamHI (RAATTY+GGATCC) double digest, 6747 thousand fragments (6382433+365999 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     295 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     431 bp
  30% of sequence in fragments shorter than     502 bp
  35% of sequence in fragments shorter than     576 bp
  40% of sequence in fragments shorter than     652 bp
  45% of sequence in fragments shorter than     730 bp
  50% of sequence in fragments shorter than     819 bp
  55% of sequence in fragments shorter than     908 bp
  60% of sequence in fragments shorter than    1011 bp
  65% of sequence in fragments shorter than    1120 bp
  70% of sequence in fragments shorter than    1253 bp
  75% of sequence in fragments shorter than    1405 bp
  80% of sequence in fragments shorter than    1602 bp
  85% of sequence in fragments shorter than    1857 bp
  90% of sequence in fragments shorter than    2245 bp
  95% of sequence in fragments shorter than    2997 bp

# AvaI.BsrI (CYCGRG+ACTGG) double digest, 6746 thousand fragments (1005714+5741310 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     493 bp
  35% of sequence in fragments shorter than     559 bp
  40% of sequence in fragments shorter than     630 bp
  45% of sequence in fragments shorter than     700 bp
  50% of sequence in fragments shorter than     773 bp
  55% of sequence in fragments shorter than     857 bp
  60% of sequence in fragments shorter than     947 bp
  65% of sequence in fragments shorter than    1048 bp
  70% of sequence in fragments shorter than    1166 bp
  75% of sequence in fragments shorter than    1303 bp
  80% of sequence in fragments shorter than    1467 bp
  85% of sequence in fragments shorter than    1681 bp
  90% of sequence in fragments shorter than    1984 bp
  95% of sequence in fragments shorter than    2533 bp

# BclI.HphI (TGATCA+GGTGA) double digest, 6745 thousand fragments (738785+6007336 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     428 bp
  30% of sequence in fragments shorter than     492 bp
  35% of sequence in fragments shorter than     556 bp
  40% of sequence in fragments shorter than     623 bp
  45% of sequence in fragments shorter than     692 bp
  50% of sequence in fragments shorter than     769 bp
  55% of sequence in fragments shorter than     852 bp
  60% of sequence in fragments shorter than     943 bp
  65% of sequence in fragments shorter than    1043 bp
  70% of sequence in fragments shorter than    1159 bp
  75% of sequence in fragments shorter than    1292 bp
  80% of sequence in fragments shorter than    1439 bp
  85% of sequence in fragments shorter than    1644 bp
  90% of sequence in fragments shorter than    1938 bp
  95% of sequence in fragments shorter than    2435 bp

# BccI.HgaI (CCATC+GACGC) double digest, 6742 thousand fragments (6170927+571889 sites), 441 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     367 bp
  25% of sequence in fragments shorter than     434 bp
  30% of sequence in fragments shorter than     501 bp
  35% of sequence in fragments shorter than     572 bp
  40% of sequence in fragments shorter than     644 bp
  45% of sequence in fragments shorter than     721 bp
  50% of sequence in fragments shorter than     804 bp
  55% of sequence in fragments shorter than     893 bp
  60% of sequence in fragments shorter than     993 bp
  65% of sequence in fragments shorter than    1099 bp
  70% of sequence in fragments shorter than    1223 bp
  75% of sequence in fragments shorter than    1371 bp
  80% of sequence in fragments shorter than    1550 bp
  85% of sequence in fragments shorter than    1785 bp
  90% of sequence in fragments shorter than    2124 bp
  95% of sequence in fragments shorter than    2742 bp

# BccI.EciI (CCATC+GGCGGA) double digest, 6741 thousand fragments (6170927+571273 sites), 442 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     302 bp
  20% of sequence in fragments shorter than     370 bp
  25% of sequence in fragments shorter than     439 bp
  30% of sequence in fragments shorter than     507 bp
  35% of sequence in fragments shorter than     580 bp
  40% of sequence in fragments shorter than     653 bp
  45% of sequence in fragments shorter than     732 bp
  50% of sequence in fragments shorter than     816 bp
  55% of sequence in fragments shorter than     906 bp
  60% of sequence in fragments shorter than    1007 bp
  65% of sequence in fragments shorter than    1113 bp
  70% of sequence in fragments shorter than    1240 bp
  75% of sequence in fragments shorter than    1390 bp
  80% of sequence in fragments shorter than    1575 bp
  85% of sequence in fragments shorter than    1815 bp
  90% of sequence in fragments shorter than    2169 bp
  95% of sequence in fragments shorter than    2818 bp

# FnuDII.HphI (CGCG+GGTGA) double digest, 6740 thousand fragments (733940+6007336 sites), 442 bp average fragment length
   5% of sequence in fragments shorter than     147 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     372 bp
  25% of sequence in fragments shorter than     444 bp
  30% of sequence in fragments shorter than     515 bp
  35% of sequence in fragments shorter than     586 bp
  40% of sequence in fragments shorter than     661 bp
  45% of sequence in fragments shorter than     738 bp
  50% of sequence in fragments shorter than     820 bp
  55% of sequence in fragments shorter than     911 bp
  60% of sequence in fragments shorter than    1014 bp
  65% of sequence in fragments shorter than    1126 bp
  70% of sequence in fragments shorter than    1254 bp
  75% of sequence in fragments shorter than    1391 bp
  80% of sequence in fragments shorter than    1572 bp
  85% of sequence in fragments shorter than    1810 bp
  90% of sequence in fragments shorter than    2136 bp
  95% of sequence in fragments shorter than    2714 bp

# HhaI.TfiI (GCGC+GAWTC) double digest, 6740 thousand fragments (1756502+4984362 sites), 442 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     306 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     437 bp
  30% of sequence in fragments shorter than     507 bp
  35% of sequence in fragments shorter than     581 bp
  40% of sequence in fragments shorter than     656 bp
  45% of sequence in fragments shorter than     730 bp
  50% of sequence in fragments shorter than     809 bp
  55% of sequence in fragments shorter than     895 bp
  60% of sequence in fragments shorter than     990 bp
  65% of sequence in fragments shorter than    1094 bp
  70% of sequence in fragments shorter than    1211 bp
  75% of sequence in fragments shorter than    1346 bp
  80% of sequence in fragments shorter than    1509 bp
  85% of sequence in fragments shorter than    1715 bp
  90% of sequence in fragments shorter than    1996 bp
  95% of sequence in fragments shorter than    2471 bp

# BccI.MfeI (CCATC+CAATTG) double digest, 6734 thousand fragments (6170927+564303 sites), 442 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     431 bp
  30% of sequence in fragments shorter than     495 bp
  35% of sequence in fragments shorter than     564 bp
  40% of sequence in fragments shorter than     635 bp
  45% of sequence in fragments shorter than     708 bp
  50% of sequence in fragments shorter than     786 bp
  55% of sequence in fragments shorter than     870 bp
  60% of sequence in fragments shorter than     962 bp
  65% of sequence in fragments shorter than    1064 bp
  70% of sequence in fragments shorter than    1174 bp
  75% of sequence in fragments shorter than    1308 bp
  80% of sequence in fragments shorter than    1474 bp
  85% of sequence in fragments shorter than    1689 bp
  90% of sequence in fragments shorter than    1992 bp
  95% of sequence in fragments shorter than    2540 bp

# BbvI.FauI (GCAGC+CCCGC) double digest, 6729 thousand fragments (5290051+1439772 sites), 442 bp average fragment length
   5% of sequence in fragments shorter than     142 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     326 bp
  20% of sequence in fragments shorter than     416 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     600 bp
  35% of sequence in fragments shorter than     695 bp
  40% of sequence in fragments shorter than     797 bp
  45% of sequence in fragments shorter than     899 bp
  50% of sequence in fragments shorter than    1012 bp
  55% of sequence in fragments shorter than    1137 bp
  60% of sequence in fragments shorter than    1277 bp
  65% of sequence in fragments shorter than    1433 bp
  70% of sequence in fragments shorter than    1614 bp
  75% of sequence in fragments shorter than    1814 bp
  80% of sequence in fragments shorter than    2066 bp
  85% of sequence in fragments shorter than    2389 bp
  90% of sequence in fragments shorter than    2860 bp
  95% of sequence in fragments shorter than    3667 bp

# BbvI.BspMI (GCAGC+ACCTGC) double digest, 6727 thousand fragments (5290051+1438129 sites), 442 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     314 bp
  20% of sequence in fragments shorter than     395 bp
  25% of sequence in fragments shorter than     478 bp
  30% of sequence in fragments shorter than     565 bp
  35% of sequence in fragments shorter than     655 bp
  40% of sequence in fragments shorter than     746 bp
  45% of sequence in fragments shorter than     845 bp
  50% of sequence in fragments shorter than     943 bp
  55% of sequence in fragments shorter than    1059 bp
  60% of sequence in fragments shorter than    1184 bp
  65% of sequence in fragments shorter than    1329 bp
  70% of sequence in fragments shorter than    1495 bp
  75% of sequence in fragments shorter than    1675 bp
  80% of sequence in fragments shorter than    1909 bp
  85% of sequence in fragments shorter than    2199 bp
  90% of sequence in fragments shorter than    2621 bp
  95% of sequence in fragments shorter than    3345 bp

# BccI.SphI (CCATC+GCATGC) double digest, 6721 thousand fragments (6170927+550952 sites), 443 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     231 bp
  15% of sequence in fragments shorter than     302 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     434 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     641 bp
  45% of sequence in fragments shorter than     717 bp
  50% of sequence in fragments shorter than     797 bp
  55% of sequence in fragments shorter than     885 bp
  60% of sequence in fragments shorter than     981 bp
  65% of sequence in fragments shorter than    1084 bp
  70% of sequence in fragments shorter than    1205 bp
  75% of sequence in fragments shorter than    1347 bp
  80% of sequence in fragments shorter than    1523 bp
  85% of sequence in fragments shorter than    1751 bp
  90% of sequence in fragments shorter than    2081 bp
  95% of sequence in fragments shorter than    2693 bp

# BsaAI.HphI (YACGTR+GGTGA) double digest, 6720 thousand fragments (713801+6007336 sites), 443 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     228 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     426 bp
  30% of sequence in fragments shorter than     491 bp
  35% of sequence in fragments shorter than     557 bp
  40% of sequence in fragments shorter than     625 bp
  45% of sequence in fragments shorter than     695 bp
  50% of sequence in fragments shorter than     772 bp
  55% of sequence in fragments shorter than     853 bp
  60% of sequence in fragments shorter than     943 bp
  65% of sequence in fragments shorter than    1042 bp
  70% of sequence in fragments shorter than    1156 bp
  75% of sequence in fragments shorter than    1291 bp
  80% of sequence in fragments shorter than    1439 bp
  85% of sequence in fragments shorter than    1637 bp
  90% of sequence in fragments shorter than    1924 bp
  95% of sequence in fragments shorter than    2411 bp

# BspHI.BsrI (TCATGA+ACTGG) double digest, 6719 thousand fragments (978290+5741310 sites), 443 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     428 bp
  30% of sequence in fragments shorter than     492 bp
  35% of sequence in fragments shorter than     559 bp
  40% of sequence in fragments shorter than     628 bp
  45% of sequence in fragments shorter than     697 bp
  50% of sequence in fragments shorter than     771 bp
  55% of sequence in fragments shorter than     853 bp
  60% of sequence in fragments shorter than     941 bp
  65% of sequence in fragments shorter than    1039 bp
  70% of sequence in fragments shorter than    1152 bp
  75% of sequence in fragments shorter than    1282 bp
  80% of sequence in fragments shorter than    1438 bp
  85% of sequence in fragments shorter than    1638 bp
  90% of sequence in fragments shorter than    1921 bp
  95% of sequence in fragments shorter than    2427 bp

# CviQI.EsaBC3I (GTAC+TCGA) double digest, 6717 thousand fragments (5115078+1603346 sites), 443 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     236 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     369 bp
  25% of sequence in fragments shorter than     431 bp
  30% of sequence in fragments shorter than     492 bp
  35% of sequence in fragments shorter than     555 bp
  40% of sequence in fragments shorter than     622 bp
  45% of sequence in fragments shorter than     690 bp
  50% of sequence in fragments shorter than     762 bp
  55% of sequence in fragments shorter than     838 bp
  60% of sequence in fragments shorter than     920 bp
  65% of sequence in fragments shorter than    1013 bp
  70% of sequence in fragments shorter than    1114 bp
  75% of sequence in fragments shorter than    1233 bp
  80% of sequence in fragments shorter than    1365 bp
  85% of sequence in fragments shorter than    1543 bp
  90% of sequence in fragments shorter than    1795 bp
  95% of sequence in fragments shorter than    2226 bp

# ApoI.BstEII (RAATTY+GGTNACC) double digest, 6717 thousand fragments (6382433+335865 sites), 443 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     297 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     435 bp
  30% of sequence in fragments shorter than     507 bp
  35% of sequence in fragments shorter than     580 bp
  40% of sequence in fragments shorter than     657 bp
  45% of sequence in fragments shorter than     736 bp
  50% of sequence in fragments shorter than     825 bp
  55% of sequence in fragments shorter than     915 bp
  60% of sequence in fragments shorter than    1020 bp
  65% of sequence in fragments shorter than    1136 bp
  70% of sequence in fragments shorter than    1272 bp
  75% of sequence in fragments shorter than    1418 bp
  80% of sequence in fragments shorter than    1618 bp
  85% of sequence in fragments shorter than    1882 bp
  90% of sequence in fragments shorter than    2265 bp
  95% of sequence in fragments shorter than    3015 bp

# BsmAI.ClaI (GTCTC+ATCGAT) double digest, 6717 thousand fragments (6631585+86477 sites), 443 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     299 bp
  20% of sequence in fragments shorter than     377 bp
  25% of sequence in fragments shorter than     453 bp
  30% of sequence in fragments shorter than     527 bp
  35% of sequence in fragments shorter than     602 bp
  40% of sequence in fragments shorter than     683 bp
  45% of sequence in fragments shorter than     770 bp
  50% of sequence in fragments shorter than     863 bp
  55% of sequence in fragments shorter than     963 bp
  60% of sequence in fragments shorter than    1073 bp
  65% of sequence in fragments shorter than    1198 bp
  70% of sequence in fragments shorter than    1342 bp
  75% of sequence in fragments shorter than    1509 bp
  80% of sequence in fragments shorter than    1714 bp
  85% of sequence in fragments shorter than    1981 bp
  90% of sequence in fragments shorter than    2353 bp
  95% of sequence in fragments shorter than    3041 bp

# BccI.ScaI (CCATC+AGTACT) double digest, 6714 thousand fragments (6170927+543793 sites), 443 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     302 bp
  20% of sequence in fragments shorter than     367 bp
  25% of sequence in fragments shorter than     432 bp
  30% of sequence in fragments shorter than     497 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     635 bp
  45% of sequence in fragments shorter than     709 bp
  50% of sequence in fragments shorter than     787 bp
  55% of sequence in fragments shorter than     871 bp
  60% of sequence in fragments shorter than     964 bp
  65% of sequence in fragments shorter than    1067 bp
  70% of sequence in fragments shorter than    1179 bp
  75% of sequence in fragments shorter than    1314 bp
  80% of sequence in fragments shorter than    1482 bp
  85% of sequence in fragments shorter than    1697 bp
  90% of sequence in fragments shorter than    2008 bp
  95% of sequence in fragments shorter than    2579 bp

# BceAI.HphI (ACGGC+GGTGA) double digest, 6712 thousand fragments (705178+6007336 sites), 443 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     226 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     362 bp
  25% of sequence in fragments shorter than     430 bp
  30% of sequence in fragments shorter than     497 bp
  35% of sequence in fragments shorter than     564 bp
  40% of sequence in fragments shorter than     634 bp
  45% of sequence in fragments shorter than     708 bp
  50% of sequence in fragments shorter than     789 bp
  55% of sequence in fragments shorter than     871 bp
  60% of sequence in fragments shorter than     968 bp
  65% of sequence in fragments shorter than    1074 bp
  70% of sequence in fragments shorter than    1199 bp
  75% of sequence in fragments shorter than    1340 bp
  80% of sequence in fragments shorter than    1500 bp
  85% of sequence in fragments shorter than    1724 bp
  90% of sequence in fragments shorter than    2035 bp
  95% of sequence in fragments shorter than    2578 bp

# ApaLI.BthCI (GTGCAC+GCNGC) double digest, 6705 thousand fragments (496255+6209929 sites), 444 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     248 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     423 bp
  25% of sequence in fragments shorter than     518 bp
  30% of sequence in fragments shorter than     613 bp
  35% of sequence in fragments shorter than     709 bp
  40% of sequence in fragments shorter than     815 bp
  45% of sequence in fragments shorter than     918 bp
  50% of sequence in fragments shorter than    1035 bp
  55% of sequence in fragments shorter than    1160 bp
  60% of sequence in fragments shorter than    1300 bp
  65% of sequence in fragments shorter than    1455 bp
  70% of sequence in fragments shorter than    1628 bp
  75% of sequence in fragments shorter than    1835 bp
  80% of sequence in fragments shorter than    2075 bp
  85% of sequence in fragments shorter than    2400 bp
  90% of sequence in fragments shorter than    2853 bp
  95% of sequence in fragments shorter than    3632 bp

# BciVI.HphI (GTATCC+GGTGA) double digest, 6702 thousand fragments (696093+6007336 sites), 444 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     227 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     361 bp
  25% of sequence in fragments shorter than     426 bp
  30% of sequence in fragments shorter than     491 bp
  35% of sequence in fragments shorter than     556 bp
  40% of sequence in fragments shorter than     623 bp
  45% of sequence in fragments shorter than     692 bp
  50% of sequence in fragments shorter than     766 bp
  55% of sequence in fragments shorter than     845 bp
  60% of sequence in fragments shorter than     933 bp
  65% of sequence in fragments shorter than    1032 bp
  70% of sequence in fragments shorter than    1143 bp
  75% of sequence in fragments shorter than    1275 bp
  80% of sequence in fragments shorter than    1426 bp
  85% of sequence in fragments shorter than    1627 bp
  90% of sequence in fragments shorter than    1915 bp
  95% of sequence in fragments shorter than    2414 bp

# AatII.BsmAI (GACGTC+GTCTC) double digest, 6702 thousand fragments (71089+6631585 sites), 444 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     455 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     606 bp
  40% of sequence in fragments shorter than     687 bp
  45% of sequence in fragments shorter than     775 bp
  50% of sequence in fragments shorter than     869 bp
  55% of sequence in fragments shorter than     971 bp
  60% of sequence in fragments shorter than    1082 bp
  65% of sequence in fragments shorter than    1208 bp
  70% of sequence in fragments shorter than    1354 bp
  75% of sequence in fragments shorter than    1524 bp
  80% of sequence in fragments shorter than    1731 bp
  85% of sequence in fragments shorter than    2001 bp
  90% of sequence in fragments shorter than    2379 bp
  95% of sequence in fragments shorter than    3076 bp

# BsmAI.SacII (GTCTC+CCGCGG) double digest, 6698 thousand fragments (6631585+66901 sites), 444 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     213 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     378 bp
  25% of sequence in fragments shorter than     455 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     606 bp
  40% of sequence in fragments shorter than     688 bp
  45% of sequence in fragments shorter than     775 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     972 bp
  60% of sequence in fragments shorter than    1084 bp
  65% of sequence in fragments shorter than    1210 bp
  70% of sequence in fragments shorter than    1358 bp
  75% of sequence in fragments shorter than    1528 bp
  80% of sequence in fragments shorter than    1737 bp
  85% of sequence in fragments shorter than    2010 bp
  90% of sequence in fragments shorter than    2390 bp
  95% of sequence in fragments shorter than    3092 bp

# BfiI.TfiI (ACTGGG+GAWTC) double digest, 6690 thousand fragments (1706594+4984362 sites), 445 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     355 bp
  25% of sequence in fragments shorter than     417 bp
  30% of sequence in fragments shorter than     484 bp
  35% of sequence in fragments shorter than     551 bp
  40% of sequence in fragments shorter than     620 bp
  45% of sequence in fragments shorter than     687 bp
  50% of sequence in fragments shorter than     760 bp
  55% of sequence in fragments shorter than     836 bp
  60% of sequence in fragments shorter than     921 bp
  65% of sequence in fragments shorter than    1015 bp
  70% of sequence in fragments shorter than    1121 bp
  75% of sequence in fragments shorter than    1245 bp
  80% of sequence in fragments shorter than    1392 bp
  85% of sequence in fragments shorter than    1580 bp
  90% of sequence in fragments shorter than    1838 bp
  95% of sequence in fragments shorter than    2265 bp

# BsmAI.PspXI (GTCTC+VCTCGAGB) double digest, 6690 thousand fragments (6631585+59193 sites), 445 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     606 bp
  40% of sequence in fragments shorter than     688 bp
  45% of sequence in fragments shorter than     775 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     971 bp
  60% of sequence in fragments shorter than    1083 bp
  65% of sequence in fragments shorter than    1209 bp
  70% of sequence in fragments shorter than    1356 bp
  75% of sequence in fragments shorter than    1525 bp
  80% of sequence in fragments shorter than    1733 bp
  85% of sequence in fragments shorter than    2004 bp
  90% of sequence in fragments shorter than    2382 bp
  95% of sequence in fragments shorter than    3079 bp

# AflIII.FokI (ACRYGT+GGATG) double digest, 6686 thousand fragments (1485394+5201116 sites), 445 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     307 bp
  20% of sequence in fragments shorter than     375 bp
  25% of sequence in fragments shorter than     443 bp
  30% of sequence in fragments shorter than     512 bp
  35% of sequence in fragments shorter than     582 bp
  40% of sequence in fragments shorter than     654 bp
  45% of sequence in fragments shorter than     730 bp
  50% of sequence in fragments shorter than     812 bp
  55% of sequence in fragments shorter than     899 bp
  60% of sequence in fragments shorter than     990 bp
  65% of sequence in fragments shorter than    1093 bp
  70% of sequence in fragments shorter than    1206 bp
  75% of sequence in fragments shorter than    1340 bp
  80% of sequence in fragments shorter than    1494 bp
  85% of sequence in fragments shorter than    1703 bp
  90% of sequence in fragments shorter than    1986 bp
  95% of sequence in fragments shorter than    2506 bp

# AgeI.BsmAI (ACCGGT+GTCTC) double digest, 6684 thousand fragments (53386+6631585 sites), 445 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     214 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     531 bp
  35% of sequence in fragments shorter than     607 bp
  40% of sequence in fragments shorter than     688 bp
  45% of sequence in fragments shorter than     776 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     972 bp
  60% of sequence in fragments shorter than    1083 bp
  65% of sequence in fragments shorter than    1209 bp
  70% of sequence in fragments shorter than    1354 bp
  75% of sequence in fragments shorter than    1525 bp
  80% of sequence in fragments shorter than    1732 bp
  85% of sequence in fragments shorter than    2002 bp
  90% of sequence in fragments shorter than    2380 bp
  95% of sequence in fragments shorter than    3077 bp

# ApoI.SexAI (RAATTY+ACCWGGT) double digest, 6679 thousand fragments (6382433+297900 sites), 446 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     298 bp
  20% of sequence in fragments shorter than     366 bp
  25% of sequence in fragments shorter than     436 bp
  30% of sequence in fragments shorter than     509 bp
  35% of sequence in fragments shorter than     583 bp
  40% of sequence in fragments shorter than     659 bp
  45% of sequence in fragments shorter than     739 bp
  50% of sequence in fragments shorter than     829 bp
  55% of sequence in fragments shorter than     920 bp
  60% of sequence in fragments shorter than    1025 bp
  65% of sequence in fragments shorter than    1143 bp
  70% of sequence in fragments shorter than    1282 bp
  75% of sequence in fragments shorter than    1430 bp
  80% of sequence in fragments shorter than    1634 bp
  85% of sequence in fragments shorter than    1903 bp
  90% of sequence in fragments shorter than    2304 bp
  95% of sequence in fragments shorter than    3096 bp

# AvaII.MlyI (GGWCC+GAGTC) double digest, 6678 thousand fragments (2747690+3931692 sites), 446 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     302 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     503 bp
  35% of sequence in fragments shorter than     575 bp
  40% of sequence in fragments shorter than     652 bp
  45% of sequence in fragments shorter than     730 bp
  50% of sequence in fragments shorter than     818 bp
  55% of sequence in fragments shorter than     910 bp
  60% of sequence in fragments shorter than    1009 bp
  65% of sequence in fragments shorter than    1125 bp
  70% of sequence in fragments shorter than    1260 bp
  75% of sequence in fragments shorter than    1411 bp
  80% of sequence in fragments shorter than    1607 bp
  85% of sequence in fragments shorter than    1858 bp
  90% of sequence in fragments shorter than    2217 bp
  95% of sequence in fragments shorter than    2883 bp

# AvaIII.BsrI (ATGCAT+ACTGG) double digest, 6674 thousand fragments (933357+5741310 sites), 446 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     231 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     367 bp
  25% of sequence in fragments shorter than     434 bp
  30% of sequence in fragments shorter than     498 bp
  35% of sequence in fragments shorter than     565 bp
  40% of sequence in fragments shorter than     633 bp
  45% of sequence in fragments shorter than     702 bp
  50% of sequence in fragments shorter than     772 bp
  55% of sequence in fragments shorter than     853 bp
  60% of sequence in fragments shorter than     939 bp
  65% of sequence in fragments shorter than    1037 bp
  70% of sequence in fragments shorter than    1148 bp
  75% of sequence in fragments shorter than    1278 bp
  80% of sequence in fragments shorter than    1431 bp
  85% of sequence in fragments shorter than    1630 bp
  90% of sequence in fragments shorter than    1905 bp
  95% of sequence in fragments shorter than    2398 bp

# BsmAI.PmeI (GTCTC+GTTTAAAC) double digest, 6673 thousand fragments (6631585+42094 sites), 446 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     215 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     531 bp
  35% of sequence in fragments shorter than     607 bp
  40% of sequence in fragments shorter than     689 bp
  45% of sequence in fragments shorter than     776 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     971 bp
  60% of sequence in fragments shorter than    1083 bp
  65% of sequence in fragments shorter than    1208 bp
  70% of sequence in fragments shorter than    1354 bp
  75% of sequence in fragments shorter than    1523 bp
  80% of sequence in fragments shorter than    1729 bp
  85% of sequence in fragments shorter than    1998 bp
  90% of sequence in fragments shorter than    2375 bp
  95% of sequence in fragments shorter than    3066 bp

# ApoI.KpnI (RAATTY+GGTACC) double digest, 6669 thousand fragments (6382433+287974 sites), 446 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     367 bp
  25% of sequence in fragments shorter than     438 bp
  30% of sequence in fragments shorter than     509 bp
  35% of sequence in fragments shorter than     583 bp
  40% of sequence in fragments shorter than     661 bp
  45% of sequence in fragments shorter than     743 bp
  50% of sequence in fragments shorter than     833 bp
  55% of sequence in fragments shorter than     921 bp
  60% of sequence in fragments shorter than    1028 bp
  65% of sequence in fragments shorter than    1146 bp
  70% of sequence in fragments shorter than    1287 bp
  75% of sequence in fragments shorter than    1448 bp
  80% of sequence in fragments shorter than    1660 bp
  85% of sequence in fragments shorter than    1940 bp
  90% of sequence in fragments shorter than    2364 bp
  95% of sequence in fragments shorter than    3210 bp

# ApaI.BthCI (GGGCCC+GCNGC) double digest, 6669 thousand fragments (460339+6209929 sites), 446 bp average fragment length
   5% of sequence in fragments shorter than     144 bp
  10% of sequence in fragments shorter than     248 bp
  15% of sequence in fragments shorter than     339 bp
  20% of sequence in fragments shorter than     433 bp
  25% of sequence in fragments shorter than     532 bp
  30% of sequence in fragments shorter than     632 bp
  35% of sequence in fragments shorter than     735 bp
  40% of sequence in fragments shorter than     844 bp
  45% of sequence in fragments shorter than     956 bp
  50% of sequence in fragments shorter than    1078 bp
  55% of sequence in fragments shorter than    1212 bp
  60% of sequence in fragments shorter than    1360 bp
  65% of sequence in fragments shorter than    1526 bp
  70% of sequence in fragments shorter than    1708 bp
  75% of sequence in fragments shorter than    1928 bp
  80% of sequence in fragments shorter than    2185 bp
  85% of sequence in fragments shorter than    2535 bp
  90% of sequence in fragments shorter than    3010 bp
  95% of sequence in fragments shorter than    3843 bp

# ApoI.NheI (RAATTY+GCTAGC) double digest, 6669 thousand fragments (6382433+287384 sites), 446 bp average fragment length
   5% of sequence in fragments shorter than     146 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     297 bp
  20% of sequence in fragments shorter than     363 bp
  25% of sequence in fragments shorter than     435 bp
  30% of sequence in fragments shorter than     509 bp
  35% of sequence in fragments shorter than     585 bp
  40% of sequence in fragments shorter than     663 bp
  45% of sequence in fragments shorter than     744 bp
  50% of sequence in fragments shorter than     835 bp
  55% of sequence in fragments shorter than     928 bp
  60% of sequence in fragments shorter than    1030 bp
  65% of sequence in fragments shorter than    1150 bp
  70% of sequence in fragments shorter than    1291 bp
  75% of sequence in fragments shorter than    1455 bp
  80% of sequence in fragments shorter than    1670 bp
  85% of sequence in fragments shorter than    1954 bp
  90% of sequence in fragments shorter than    2388 bp
  95% of sequence in fragments shorter than    3280 bp

# ApaLI.BccI (GTGCAC+CCATC) double digest, 6666 thousand fragments (496255+6170927 sites), 446 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     233 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     370 bp
  25% of sequence in fragments shorter than     437 bp
  30% of sequence in fragments shorter than     503 bp
  35% of sequence in fragments shorter than     574 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     722 bp
  50% of sequence in fragments shorter than     804 bp
  55% of sequence in fragments shorter than     892 bp
  60% of sequence in fragments shorter than     990 bp
  65% of sequence in fragments shorter than    1094 bp
  70% of sequence in fragments shorter than    1216 bp
  75% of sequence in fragments shorter than    1361 bp
  80% of sequence in fragments shorter than    1540 bp
  85% of sequence in fragments shorter than    1771 bp
  90% of sequence in fragments shorter than    2107 bp
  95% of sequence in fragments shorter than    2722 bp

# BsrI.StuI (ACTGG+AGGCCT) double digest, 6666 thousand fragments (5741310+925729 sites), 447 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     436 bp
  30% of sequence in fragments shorter than     503 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     642 bp
  45% of sequence in fragments shorter than     709 bp
  50% of sequence in fragments shorter than     786 bp
  55% of sequence in fragments shorter than     869 bp
  60% of sequence in fragments shorter than     962 bp
  65% of sequence in fragments shorter than    1065 bp
  70% of sequence in fragments shorter than    1184 bp
  75% of sequence in fragments shorter than    1319 bp
  80% of sequence in fragments shorter than    1480 bp
  85% of sequence in fragments shorter than    1686 bp
  90% of sequence in fragments shorter than    1974 bp
  95% of sequence in fragments shorter than    2464 bp

# CviQI.HgiCI (GTAC+GGYRCC) double digest, 6665 thousand fragments (5115078+1550877 sites), 447 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     320 bp
  20% of sequence in fragments shorter than     388 bp
  25% of sequence in fragments shorter than     455 bp
  30% of sequence in fragments shorter than     518 bp
  35% of sequence in fragments shorter than     584 bp
  40% of sequence in fragments shorter than     653 bp
  45% of sequence in fragments shorter than     723 bp
  50% of sequence in fragments shorter than     798 bp
  55% of sequence in fragments shorter than     880 bp
  60% of sequence in fragments shorter than     967 bp
  65% of sequence in fragments shorter than    1063 bp
  70% of sequence in fragments shorter than    1175 bp
  75% of sequence in fragments shorter than    1297 bp
  80% of sequence in fragments shorter than    1434 bp
  85% of sequence in fragments shorter than    1626 bp
  90% of sequence in fragments shorter than    1902 bp
  95% of sequence in fragments shorter than    2388 bp

# BsmI.CviQI (GAATGC+GTAC) double digest, 6663 thousand fragments (1549350+5115078 sites), 447 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     443 bp
  30% of sequence in fragments shorter than     505 bp
  35% of sequence in fragments shorter than     575 bp
  40% of sequence in fragments shorter than     647 bp
  45% of sequence in fragments shorter than     720 bp
  50% of sequence in fragments shorter than     797 bp
  55% of sequence in fragments shorter than     882 bp
  60% of sequence in fragments shorter than     971 bp
  65% of sequence in fragments shorter than    1067 bp
  70% of sequence in fragments shorter than    1182 bp
  75% of sequence in fragments shorter than    1313 bp
  80% of sequence in fragments shorter than    1456 bp
  85% of sequence in fragments shorter than    1652 bp
  90% of sequence in fragments shorter than    1928 bp
  95% of sequence in fragments shorter than    2393 bp

# BsmAI.SalI (GTCTC+GTCGAC) double digest, 6662 thousand fragments (6631585+31396 sites), 447 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     215 bp
  15% of sequence in fragments shorter than     302 bp
  20% of sequence in fragments shorter than     380 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     532 bp
  35% of sequence in fragments shorter than     609 bp
  40% of sequence in fragments shorter than     690 bp
  45% of sequence in fragments shorter than     778 bp
  50% of sequence in fragments shorter than     873 bp
  55% of sequence in fragments shorter than     975 bp
  60% of sequence in fragments shorter than    1087 bp
  65% of sequence in fragments shorter than    1212 bp
  70% of sequence in fragments shorter than    1360 bp
  75% of sequence in fragments shorter than    1530 bp
  80% of sequence in fragments shorter than    1738 bp
  85% of sequence in fragments shorter than    2009 bp
  90% of sequence in fragments shorter than    2387 bp
  95% of sequence in fragments shorter than    3085 bp

# MlyI.SmlI (GAGTC+CTYRAG) double digest, 6659 thousand fragments (3931692+2727867 sites), 447 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     434 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     564 bp
  40% of sequence in fragments shorter than     633 bp
  45% of sequence in fragments shorter than     704 bp
  50% of sequence in fragments shorter than     780 bp
  55% of sequence in fragments shorter than     862 bp
  60% of sequence in fragments shorter than     953 bp
  65% of sequence in fragments shorter than    1051 bp
  70% of sequence in fragments shorter than    1168 bp
  75% of sequence in fragments shorter than    1308 bp
  80% of sequence in fragments shorter than    1473 bp
  85% of sequence in fragments shorter than    1696 bp
  90% of sequence in fragments shorter than    2002 bp
  95% of sequence in fragments shorter than    2574 bp

# BthCI.EcoRV (GCNGC+GATATC) double digest, 6655 thousand fragments (6209929+446473 sites), 447 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     337 bp
  20% of sequence in fragments shorter than     425 bp
  25% of sequence in fragments shorter than     514 bp
  30% of sequence in fragments shorter than     606 bp
  35% of sequence in fragments shorter than     698 bp
  40% of sequence in fragments shorter than     799 bp
  45% of sequence in fragments shorter than     896 bp
  50% of sequence in fragments shorter than    1009 bp
  55% of sequence in fragments shorter than    1125 bp
  60% of sequence in fragments shorter than    1253 bp
  65% of sequence in fragments shorter than    1396 bp
  70% of sequence in fragments shorter than    1564 bp
  75% of sequence in fragments shorter than    1746 bp
  80% of sequence in fragments shorter than    1978 bp
  85% of sequence in fragments shorter than    2268 bp
  90% of sequence in fragments shorter than    2683 bp
  95% of sequence in fragments shorter than    3382 bp

# BsmAI.MluI (GTCTC+ACGCGT) double digest, 6652 thousand fragments (6631585+21415 sites), 447 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     215 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     533 bp
  35% of sequence in fragments shorter than     610 bp
  40% of sequence in fragments shorter than     692 bp
  45% of sequence in fragments shorter than     780 bp
  50% of sequence in fragments shorter than     875 bp
  55% of sequence in fragments shorter than     977 bp
  60% of sequence in fragments shorter than    1090 bp
  65% of sequence in fragments shorter than    1215 bp
  70% of sequence in fragments shorter than    1363 bp
  75% of sequence in fragments shorter than    1534 bp
  80% of sequence in fragments shorter than    1742 bp
  85% of sequence in fragments shorter than    2014 bp
  90% of sequence in fragments shorter than    2393 bp
  95% of sequence in fragments shorter than    3093 bp

# AflII.HphI (CTTAAG+GGTGA) double digest, 6650 thousand fragments (643284+6007336 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     301 bp
  20% of sequence in fragments shorter than     364 bp
  25% of sequence in fragments shorter than     430 bp
  30% of sequence in fragments shorter than     495 bp
  35% of sequence in fragments shorter than     560 bp
  40% of sequence in fragments shorter than     628 bp
  45% of sequence in fragments shorter than     698 bp
  50% of sequence in fragments shorter than     775 bp
  55% of sequence in fragments shorter than     855 bp
  60% of sequence in fragments shorter than     944 bp
  65% of sequence in fragments shorter than    1043 bp
  70% of sequence in fragments shorter than    1156 bp
  75% of sequence in fragments shorter than    1288 bp
  80% of sequence in fragments shorter than    1436 bp
  85% of sequence in fragments shorter than    1643 bp
  90% of sequence in fragments shorter than    1937 bp
  95% of sequence in fragments shorter than    2425 bp

# BsmAI.SgrAI (GTCTC+CRCCGGYG) double digest, 6646 thousand fragments (6631585+15270 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     692 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     876 bp
  55% of sequence in fragments shorter than     978 bp
  60% of sequence in fragments shorter than    1090 bp
  65% of sequence in fragments shorter than    1216 bp
  70% of sequence in fragments shorter than    1364 bp
  75% of sequence in fragments shorter than    1535 bp
  80% of sequence in fragments shorter than    1744 bp
  85% of sequence in fragments shorter than    2016 bp
  90% of sequence in fragments shorter than    2396 bp
  95% of sequence in fragments shorter than    3097 bp

# BsmAI.NruI (GTCTC+TCGCGA) double digest, 6645 thousand fragments (6631585+14872 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     876 bp
  55% of sequence in fragments shorter than     979 bp
  60% of sequence in fragments shorter than    1091 bp
  65% of sequence in fragments shorter than    1217 bp
  70% of sequence in fragments shorter than    1365 bp
  75% of sequence in fragments shorter than    1536 bp
  80% of sequence in fragments shorter than    1745 bp
  85% of sequence in fragments shorter than    2016 bp
  90% of sequence in fragments shorter than    2396 bp
  95% of sequence in fragments shorter than    3095 bp

# BsmAI.PvuI (GTCTC+CGATCG) double digest, 6645 thousand fragments (6631585+14186 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     875 bp
  55% of sequence in fragments shorter than     978 bp
  60% of sequence in fragments shorter than    1090 bp
  65% of sequence in fragments shorter than    1216 bp
  70% of sequence in fragments shorter than    1364 bp
  75% of sequence in fragments shorter than    1535 bp
  80% of sequence in fragments shorter than    1744 bp
  85% of sequence in fragments shorter than    2016 bp
  90% of sequence in fragments shorter than    2395 bp
  95% of sequence in fragments shorter than    3095 bp

# BsmAI.FseI (GTCTC+GGCCGGCC) double digest, 6644 thousand fragments (6631585+13662 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     876 bp
  55% of sequence in fragments shorter than     978 bp
  60% of sequence in fragments shorter than    1091 bp
  65% of sequence in fragments shorter than    1217 bp
  70% of sequence in fragments shorter than    1365 bp
  75% of sequence in fragments shorter than    1536 bp
  80% of sequence in fragments shorter than    1745 bp
  85% of sequence in fragments shorter than    2017 bp
  90% of sequence in fragments shorter than    2397 bp
  95% of sequence in fragments shorter than    3099 bp

# SfeI.StyI (CTRYAG+CCWWGG) double digest, 6644 thousand fragments (3531009+3114107 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     229 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     365 bp
  25% of sequence in fragments shorter than     432 bp
  30% of sequence in fragments shorter than     499 bp
  35% of sequence in fragments shorter than     567 bp
  40% of sequence in fragments shorter than     636 bp
  45% of sequence in fragments shorter than     708 bp
  50% of sequence in fragments shorter than     784 bp
  55% of sequence in fragments shorter than     870 bp
  60% of sequence in fragments shorter than     964 bp
  65% of sequence in fragments shorter than    1066 bp
  70% of sequence in fragments shorter than    1184 bp
  75% of sequence in fragments shorter than    1324 bp
  80% of sequence in fragments shorter than    1490 bp
  85% of sequence in fragments shorter than    1709 bp
  90% of sequence in fragments shorter than    1988 bp
  95% of sequence in fragments shorter than    2517 bp

# BsrI.NdeI (ACTGG+CATATG) double digest, 6644 thousand fragments (5741310+903360 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     369 bp
  25% of sequence in fragments shorter than     437 bp
  30% of sequence in fragments shorter than     502 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     638 bp
  45% of sequence in fragments shorter than     708 bp
  50% of sequence in fragments shorter than     783 bp
  55% of sequence in fragments shorter than     865 bp
  60% of sequence in fragments shorter than     954 bp
  65% of sequence in fragments shorter than    1053 bp
  70% of sequence in fragments shorter than    1168 bp
  75% of sequence in fragments shorter than    1300 bp
  80% of sequence in fragments shorter than    1456 bp
  85% of sequence in fragments shorter than    1661 bp
  90% of sequence in fragments shorter than    1946 bp
  95% of sequence in fragments shorter than    2455 bp

# BsmAI.NotI (GTCTC+GCGGCCGC) double digest, 6640 thousand fragments (6631585+9795 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     877 bp
  55% of sequence in fragments shorter than     979 bp
  60% of sequence in fragments shorter than    1092 bp
  65% of sequence in fragments shorter than    1218 bp
  70% of sequence in fragments shorter than    1366 bp
  75% of sequence in fragments shorter than    1537 bp
  80% of sequence in fragments shorter than    1746 bp
  85% of sequence in fragments shorter than    2018 bp
  90% of sequence in fragments shorter than    2398 bp
  95% of sequence in fragments shorter than    3100 bp

# BsmAI.RsrII (GTCTC+CGGWCCG) double digest, 6640 thousand fragments (6631585+9764 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     877 bp
  55% of sequence in fragments shorter than     979 bp
  60% of sequence in fragments shorter than    1092 bp
  65% of sequence in fragments shorter than    1218 bp
  70% of sequence in fragments shorter than    1366 bp
  75% of sequence in fragments shorter than    1537 bp
  80% of sequence in fragments shorter than    1746 bp
  85% of sequence in fragments shorter than    2018 bp
  90% of sequence in fragments shorter than    2398 bp
  95% of sequence in fragments shorter than    3099 bp

# FauI.FokI (CCCGC+GGATG) double digest, 6640 thousand fragments (1439772+5201116 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     239 bp
  15% of sequence in fragments shorter than     318 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     467 bp
  30% of sequence in fragments shorter than     545 bp
  35% of sequence in fragments shorter than     623 bp
  40% of sequence in fragments shorter than     705 bp
  45% of sequence in fragments shorter than     792 bp
  50% of sequence in fragments shorter than     882 bp
  55% of sequence in fragments shorter than     979 bp
  60% of sequence in fragments shorter than    1087 bp
  65% of sequence in fragments shorter than    1205 bp
  70% of sequence in fragments shorter than    1340 bp
  75% of sequence in fragments shorter than    1491 bp
  80% of sequence in fragments shorter than    1683 bp
  85% of sequence in fragments shorter than    1930 bp
  90% of sequence in fragments shorter than    2283 bp
  95% of sequence in fragments shorter than    2913 bp

# BspMI.FokI (ACCTGC+GGATG) double digest, 6638 thousand fragments (1438129+5201116 sites), 448 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     306 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     445 bp
  30% of sequence in fragments shorter than     516 bp
  35% of sequence in fragments shorter than     589 bp
  40% of sequence in fragments shorter than     662 bp
  45% of sequence in fragments shorter than     741 bp
  50% of sequence in fragments shorter than     822 bp
  55% of sequence in fragments shorter than     911 bp
  60% of sequence in fragments shorter than    1004 bp
  65% of sequence in fragments shorter than    1111 bp
  70% of sequence in fragments shorter than    1233 bp
  75% of sequence in fragments shorter than    1374 bp
  80% of sequence in fragments shorter than    1544 bp
  85% of sequence in fragments shorter than    1765 bp
  90% of sequence in fragments shorter than    2075 bp
  95% of sequence in fragments shorter than    2623 bp

# HaeII.HphI (RGCGCY+GGTGA) double digest, 6636 thousand fragments (630101+6007336 sites), 449 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     231 bp
  15% of sequence in fragments shorter than     306 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     438 bp
  30% of sequence in fragments shorter than     507 bp
  35% of sequence in fragments shorter than     577 bp
  40% of sequence in fragments shorter than     650 bp
  45% of sequence in fragments shorter than     725 bp
  50% of sequence in fragments shorter than     805 bp
  55% of sequence in fragments shorter than     893 bp
  60% of sequence in fragments shorter than     993 bp
  65% of sequence in fragments shorter than    1101 bp
  70% of sequence in fragments shorter than    1228 bp
  75% of sequence in fragments shorter than    1370 bp
  80% of sequence in fragments shorter than    1537 bp
  85% of sequence in fragments shorter than    1767 bp
  90% of sequence in fragments shorter than    2081 bp
  95% of sequence in fragments shorter than    2617 bp

# AscI.BsmAI (GGCGCGCC+GTCTC) double digest, 6635 thousand fragments (4751+6631585 sites), 449 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     216 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     382 bp
  25% of sequence in fragments shorter than     460 bp
  30% of sequence in fragments shorter than     535 bp
  35% of sequence in fragments shorter than     612 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     877 bp
  55% of sequence in fragments shorter than     980 bp
  60% of sequence in fragments shorter than    1092 bp
  65% of sequence in fragments shorter than    1219 bp
  70% of sequence in fragments shorter than    1367 bp
  75% of sequence in fragments shorter than    1538 bp
  80% of sequence in fragments shorter than    1747 bp
  85% of sequence in fragments shorter than    2019 bp
  90% of sequence in fragments shorter than    2399 bp
  95% of sequence in fragments shorter than    3100 bp

# NspI.SfeI (RCATGY+CTRYAG) double digest, 6634 thousand fragments (3104359+3531009 sites), 449 bp average fragment length
   5% of sequence in fragments shorter than     164 bp
  10% of sequence in fragments shorter than     239 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     436 bp
  30% of sequence in fragments shorter than     495 bp
  35% of sequence in fragments shorter than     560 bp
  40% of sequence in fragments shorter than     628 bp
  45% of sequence in fragments shorter than     695 bp
  50% of sequence in fragments shorter than     769 bp
  55% of sequence in fragments shorter than     849 bp
  60% of sequence in fragments shorter than     936 bp
  65% of sequence in fragments shorter than    1029 bp
  70% of sequence in fragments shorter than    1136 bp
  75% of sequence in fragments shorter than    1263 bp
  80% of sequence in fragments shorter than    1410 bp
  85% of sequence in fragments shorter than    1605 bp
  90% of sequence in fragments shorter than    1880 bp
  95% of sequence in fragments shorter than    2313 bp

# PsiI.TfiI (TTATAA+GAWTC) double digest, 6631 thousand fragments (1647912+4984362 sites), 449 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     233 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     354 bp
  25% of sequence in fragments shorter than     420 bp
  30% of sequence in fragments shorter than     486 bp
  35% of sequence in fragments shorter than     553 bp
  40% of sequence in fragments shorter than     623 bp
  45% of sequence in fragments shorter than     692 bp
  50% of sequence in fragments shorter than     767 bp
  55% of sequence in fragments shorter than     845 bp
  60% of sequence in fragments shorter than     934 bp
  65% of sequence in fragments shorter than    1033 bp
  70% of sequence in fragments shorter than    1143 bp
  75% of sequence in fragments shorter than    1274 bp
  80% of sequence in fragments shorter than    1431 bp
  85% of sequence in fragments shorter than    1635 bp
  90% of sequence in fragments shorter than    1913 bp
  95% of sequence in fragments shorter than    2401 bp

# ApaI.BccI (GGGCCC+CCATC) double digest, 6630 thousand fragments (460339+6170927 sites), 449 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     233 bp
  15% of sequence in fragments shorter than     306 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     441 bp
  30% of sequence in fragments shorter than     509 bp
  35% of sequence in fragments shorter than     583 bp
  40% of sequence in fragments shorter than     656 bp
  45% of sequence in fragments shorter than     735 bp
  50% of sequence in fragments shorter than     819 bp
  55% of sequence in fragments shorter than     911 bp
  60% of sequence in fragments shorter than    1012 bp
  65% of sequence in fragments shorter than    1119 bp
  70% of sequence in fragments shorter than    1247 bp
  75% of sequence in fragments shorter than    1399 bp
  80% of sequence in fragments shorter than    1585 bp
  85% of sequence in fragments shorter than    1827 bp
  90% of sequence in fragments shorter than    2184 bp
  95% of sequence in fragments shorter than    2844 bp

# ApoI.BsrBI (RAATTY+CCGCTC) double digest, 6617 thousand fragments (6382433+235523 sites), 450 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     302 bp
  20% of sequence in fragments shorter than     371 bp
  25% of sequence in fragments shorter than     441 bp
  30% of sequence in fragments shorter than     515 bp
  35% of sequence in fragments shorter than     589 bp
  40% of sequence in fragments shorter than     666 bp
  45% of sequence in fragments shorter than     746 bp
  50% of sequence in fragments shorter than     835 bp
  55% of sequence in fragments shorter than     926 bp
  60% of sequence in fragments shorter than    1031 bp
  65% of sequence in fragments shorter than    1147 bp
  70% of sequence in fragments shorter than    1283 bp
  75% of sequence in fragments shorter than    1428 bp
  80% of sequence in fragments shorter than    1623 bp
  85% of sequence in fragments shorter than    1880 bp
  90% of sequence in fragments shorter than    2251 bp
  95% of sequence in fragments shorter than    2957 bp

# BccI.EcoRV (CCATC+GATATC) double digest, 6616 thousand fragments (6170927+446473 sites), 450 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     308 bp
  20% of sequence in fragments shorter than     374 bp
  25% of sequence in fragments shorter than     440 bp
  30% of sequence in fragments shorter than     507 bp
  35% of sequence in fragments shorter than     577 bp
  40% of sequence in fragments shorter than     648 bp
  45% of sequence in fragments shorter than     722 bp
  50% of sequence in fragments shorter than     802 bp
  55% of sequence in fragments shorter than     888 bp
  60% of sequence in fragments shorter than     984 bp
  65% of sequence in fragments shorter than    1085 bp
  70% of sequence in fragments shorter than    1203 bp
  75% of sequence in fragments shorter than    1342 bp
  80% of sequence in fragments shorter than    1512 bp
  85% of sequence in fragments shorter than    1729 bp
  90% of sequence in fragments shorter than    2041 bp
  95% of sequence in fragments shorter than    2607 bp

# ApoI.KasI (RAATTY+GGCGCC) double digest, 6612 thousand fragments (6382433+230381 sites), 450 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     300 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     439 bp
  30% of sequence in fragments shorter than     512 bp
  35% of sequence in fragments shorter than     586 bp
  40% of sequence in fragments shorter than     662 bp
  45% of sequence in fragments shorter than     742 bp
  50% of sequence in fragments shorter than     831 bp
  55% of sequence in fragments shorter than     921 bp
  60% of sequence in fragments shorter than    1026 bp
  65% of sequence in fragments shorter than    1144 bp
  70% of sequence in fragments shorter than    1280 bp
  75% of sequence in fragments shorter than    1426 bp
  80% of sequence in fragments shorter than    1625 bp
  85% of sequence in fragments shorter than    1890 bp
  90% of sequence in fragments shorter than    2275 bp
  95% of sequence in fragments shorter than    3032 bp

# BbvI.PstI (GCAGC+CTGCAG) double digest, 6611 thousand fragments (5290051+1321469 sites), 450 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     264 bp
  15% of sequence in fragments shorter than     357 bp
  20% of sequence in fragments shorter than     451 bp
  25% of sequence in fragments shorter than     546 bp
  30% of sequence in fragments shorter than     645 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     849 bp
  45% of sequence in fragments shorter than     958 bp
  50% of sequence in fragments shorter than    1076 bp
  55% of sequence in fragments shorter than    1207 bp
  60% of sequence in fragments shorter than    1351 bp
  65% of sequence in fragments shorter than    1512 bp
  70% of sequence in fragments shorter than    1684 bp
  75% of sequence in fragments shorter than    1894 bp
  80% of sequence in fragments shorter than    2136 bp
  85% of sequence in fragments shorter than    2465 bp
  90% of sequence in fragments shorter than    2932 bp
  95% of sequence in fragments shorter than    3739 bp

# HphI.SacI (GGTGA+GAGCTC) double digest, 6610 thousand fragments (6007336+603323 sites), 450 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     231 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     436 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     572 bp
  40% of sequence in fragments shorter than     641 bp
  45% of sequence in fragments shorter than     714 bp
  50% of sequence in fragments shorter than     794 bp
  55% of sequence in fragments shorter than     876 bp
  60% of sequence in fragments shorter than     973 bp
  65% of sequence in fragments shorter than    1078 bp
  70% of sequence in fragments shorter than    1201 bp
  75% of sequence in fragments shorter than    1338 bp
  80% of sequence in fragments shorter than    1497 bp
  85% of sequence in fragments shorter than    1720 bp
  90% of sequence in fragments shorter than    2033 bp
  95% of sequence in fragments shorter than    2579 bp

# BthCI.SpeI (GCNGC+ACTAGT) double digest, 6609 thousand fragments (6209929+400286 sites), 450 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     250 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     430 bp
  25% of sequence in fragments shorter than     523 bp
  30% of sequence in fragments shorter than     616 bp
  35% of sequence in fragments shorter than     711 bp
  40% of sequence in fragments shorter than     814 bp
  45% of sequence in fragments shorter than     913 bp
  50% of sequence in fragments shorter than    1027 bp
  55% of sequence in fragments shorter than    1148 bp
  60% of sequence in fragments shorter than    1281 bp
  65% of sequence in fragments shorter than    1431 bp
  70% of sequence in fragments shorter than    1603 bp
  75% of sequence in fragments shorter than    1792 bp
  80% of sequence in fragments shorter than    2034 bp
  85% of sequence in fragments shorter than    2329 bp
  90% of sequence in fragments shorter than    2756 bp
  95% of sequence in fragments shorter than    3482 bp

# ApoI.SwaI (RAATTY+ATTTAAAT) double digest, 6608 thousand fragments (6382433+226602 sites), 450 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     233 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     374 bp
  25% of sequence in fragments shorter than     447 bp
  30% of sequence in fragments shorter than     523 bp
  35% of sequence in fragments shorter than     601 bp
  40% of sequence in fragments shorter than     682 bp
  45% of sequence in fragments shorter than     767 bp
  50% of sequence in fragments shorter than     861 bp
  55% of sequence in fragments shorter than     958 bp
  60% of sequence in fragments shorter than    1071 bp
  65% of sequence in fragments shorter than    1201 bp
  70% of sequence in fragments shorter than    1341 bp
  75% of sequence in fragments shorter than    1515 bp
  80% of sequence in fragments shorter than    1741 bp
  85% of sequence in fragments shorter than    2052 bp
  90% of sequence in fragments shorter than    2528 bp
  95% of sequence in fragments shorter than    3531 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# AvrII.HphI (CCTAGG+GGTGA) double digest, 6601 thousand fragments (594956+6007336 sites), 451 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     233 bp
  15% of sequence in fragments shorter than     306 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     435 bp
  30% of sequence in fragments shorter than     503 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     639 bp
  45% of sequence in fragments shorter than     709 bp
  50% of sequence in fragments shorter than     789 bp
  55% of sequence in fragments shorter than     871 bp
  60% of sequence in fragments shorter than     965 bp
  65% of sequence in fragments shorter than    1068 bp
  70% of sequence in fragments shorter than    1188 bp
  75% of sequence in fragments shorter than    1327 bp
  80% of sequence in fragments shorter than    1484 bp
  85% of sequence in fragments shorter than    1700 bp
  90% of sequence in fragments shorter than    2005 bp
  95% of sequence in fragments shorter than    2530 bp

# BsrI.HindIII (ACTGG+AAGCTT) double digest, 6601 thousand fragments (5741310+860365 sites), 451 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     233 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     371 bp
  25% of sequence in fragments shorter than     440 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     571 bp
  40% of sequence in fragments shorter than     641 bp
  45% of sequence in fragments shorter than     710 bp
  50% of sequence in fragments shorter than     784 bp
  55% of sequence in fragments shorter than     867 bp
  60% of sequence in fragments shorter than     956 bp
  65% of sequence in fragments shorter than    1055 bp
  70% of sequence in fragments shorter than    1169 bp
  75% of sequence in fragments shorter than    1300 bp
  80% of sequence in fragments shorter than    1458 bp
  85% of sequence in fragments shorter than    1663 bp
  90% of sequence in fragments shorter than    1946 bp
  95% of sequence in fragments shorter than    2450 bp

# AflIII.CviQI (ACRYGT+GTAC) double digest, 6599 thousand fragments (1485394+5115078 sites), 451 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     324 bp
  20% of sequence in fragments shorter than     395 bp
  25% of sequence in fragments shorter than     463 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     600 bp
  40% of sequence in fragments shorter than     672 bp
  45% of sequence in fragments shorter than     745 bp
  50% of sequence in fragments shorter than     824 bp
  55% of sequence in fragments shorter than     908 bp
  60% of sequence in fragments shorter than     998 bp
  65% of sequence in fragments shorter than    1100 bp
  70% of sequence in fragments shorter than    1214 bp
  75% of sequence in fragments shorter than    1343 bp
  80% of sequence in fragments shorter than    1505 bp
  85% of sequence in fragments shorter than    1712 bp
  90% of sequence in fragments shorter than    2010 bp
  95% of sequence in fragments shorter than    2520 bp

# BthCI.HpaI (GCNGC+GTTAAC) double digest, 6598 thousand fragments (6209929+389418 sites), 451 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     429 bp
  25% of sequence in fragments shorter than     522 bp
  30% of sequence in fragments shorter than     615 bp
  35% of sequence in fragments shorter than     709 bp
  40% of sequence in fragments shorter than     812 bp
  45% of sequence in fragments shorter than     911 bp
  50% of sequence in fragments shorter than    1025 bp
  55% of sequence in fragments shorter than    1143 bp
  60% of sequence in fragments shorter than    1273 bp
  65% of sequence in fragments shorter than    1422 bp
  70% of sequence in fragments shorter than    1594 bp
  75% of sequence in fragments shorter than    1783 bp
  80% of sequence in fragments shorter than    2024 bp
  85% of sequence in fragments shorter than    2326 bp
  90% of sequence in fragments shorter than    2756 bp
  95% of sequence in fragments shorter than    3482 bp

# BsrI.XbaI (ACTGG+TCTAGA) double digest, 6591 thousand fragments (5741310+850998 sites), 452 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     308 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     442 bp
  30% of sequence in fragments shorter than     506 bp
  35% of sequence in fragments shorter than     572 bp
  40% of sequence in fragments shorter than     643 bp
  45% of sequence in fragments shorter than     711 bp
  50% of sequence in fragments shorter than     787 bp
  55% of sequence in fragments shorter than     868 bp
  60% of sequence in fragments shorter than     959 bp
  65% of sequence in fragments shorter than    1055 bp
  70% of sequence in fragments shorter than    1167 bp
  75% of sequence in fragments shorter than    1297 bp
  80% of sequence in fragments shorter than    1451 bp
  85% of sequence in fragments shorter than    1648 bp
  90% of sequence in fragments shorter than    1920 bp
  95% of sequence in fragments shorter than    2392 bp

# BsrI.EcoRI (ACTGG+GAATTC) double digest, 6588 thousand fragments (5741310+847401 sites), 452 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     307 bp
  20% of sequence in fragments shorter than     371 bp
  25% of sequence in fragments shorter than     440 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     572 bp
  40% of sequence in fragments shorter than     643 bp
  45% of sequence in fragments shorter than     710 bp
  50% of sequence in fragments shorter than     784 bp
  55% of sequence in fragments shorter than     865 bp
  60% of sequence in fragments shorter than     956 bp
  65% of sequence in fragments shorter than    1057 bp
  70% of sequence in fragments shorter than    1172 bp
  75% of sequence in fragments shorter than    1305 bp
  80% of sequence in fragments shorter than    1456 bp
  85% of sequence in fragments shorter than    1657 bp
  90% of sequence in fragments shorter than    1941 bp
  95% of sequence in fragments shorter than    2436 bp

# EsaBC3I.TfiI (TCGA+GAWTC) double digest, 6587 thousand fragments (1603346+4984362 sites), 452 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     239 bp
  15% of sequence in fragments shorter than     313 bp
  20% of sequence in fragments shorter than     367 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     641 bp
  45% of sequence in fragments shorter than     711 bp
  50% of sequence in fragments shorter than     787 bp
  55% of sequence in fragments shorter than     869 bp
  60% of sequence in fragments shorter than     958 bp
  65% of sequence in fragments shorter than    1056 bp
  70% of sequence in fragments shorter than    1168 bp
  75% of sequence in fragments shorter than    1297 bp
  80% of sequence in fragments shorter than    1451 bp
  85% of sequence in fragments shorter than    1647 bp
  90% of sequence in fragments shorter than    1915 bp
  95% of sequence in fragments shorter than    2364 bp

# BthCI.SapI (GCNGC+GCTCTTC) double digest, 6586 thousand fragments (6209929+377161 sites), 452 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     435 bp
  25% of sequence in fragments shorter than     531 bp
  30% of sequence in fragments shorter than     626 bp
  35% of sequence in fragments shorter than     724 bp
  40% of sequence in fragments shorter than     829 bp
  45% of sequence in fragments shorter than     936 bp
  50% of sequence in fragments shorter than    1052 bp
  55% of sequence in fragments shorter than    1182 bp
  60% of sequence in fragments shorter than    1323 bp
  65% of sequence in fragments shorter than    1471 bp
  70% of sequence in fragments shorter than    1646 bp
  75% of sequence in fragments shorter than    1858 bp
  80% of sequence in fragments shorter than    2109 bp
  85% of sequence in fragments shorter than    2439 bp
  90% of sequence in fragments shorter than    2903 bp
  95% of sequence in fragments shorter than    3689 bp

# BthCI.SmaI (GCNGC+CCCGGG) double digest, 6586 thousand fragments (6209929+376940 sites), 452 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     255 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     436 bp
  25% of sequence in fragments shorter than     532 bp
  30% of sequence in fragments shorter than     628 bp
  35% of sequence in fragments shorter than     728 bp
  40% of sequence in fragments shorter than     833 bp
  45% of sequence in fragments shorter than     941 bp
  50% of sequence in fragments shorter than    1060 bp
  55% of sequence in fragments shorter than    1191 bp
  60% of sequence in fragments shorter than    1334 bp
  65% of sequence in fragments shorter than    1495 bp
  70% of sequence in fragments shorter than    1673 bp
  75% of sequence in fragments shorter than    1889 bp
  80% of sequence in fragments shorter than    2137 bp
  85% of sequence in fragments shorter than    2478 bp
  90% of sequence in fragments shorter than    2944 bp
  95% of sequence in fragments shorter than    3763 bp

# AciI.SspI (CCGC+AATATT) double digest, 6582 thousand fragments (4206130+2377267 sites), 452 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     254 bp
  15% of sequence in fragments shorter than     344 bp
  20% of sequence in fragments shorter than     430 bp
  25% of sequence in fragments shorter than     514 bp
  30% of sequence in fragments shorter than     598 bp
  35% of sequence in fragments shorter than     686 bp
  40% of sequence in fragments shorter than     773 bp
  45% of sequence in fragments shorter than     865 bp
  50% of sequence in fragments shorter than     963 bp
  55% of sequence in fragments shorter than    1066 bp
  60% of sequence in fragments shorter than    1182 bp
  65% of sequence in fragments shorter than    1310 bp
  70% of sequence in fragments shorter than    1449 bp
  75% of sequence in fragments shorter than    1618 bp
  80% of sequence in fragments shorter than    1826 bp
  85% of sequence in fragments shorter than    2085 bp
  90% of sequence in fragments shorter than    2449 bp
  95% of sequence in fragments shorter than    3077 bp

# HgaI.HphI (GACGC+GGTGA) double digest, 6578 thousand fragments (571889+6007336 sites), 452 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     371 bp
  25% of sequence in fragments shorter than     440 bp
  30% of sequence in fragments shorter than     509 bp
  35% of sequence in fragments shorter than     578 bp
  40% of sequence in fragments shorter than     649 bp
  45% of sequence in fragments shorter than     724 bp
  50% of sequence in fragments shorter than     804 bp
  55% of sequence in fragments shorter than     892 bp
  60% of sequence in fragments shorter than     991 bp
  65% of sequence in fragments shorter than    1100 bp
  70% of sequence in fragments shorter than    1226 bp
  75% of sequence in fragments shorter than    1369 bp
  80% of sequence in fragments shorter than    1535 bp
  85% of sequence in fragments shorter than    1766 bp
  90% of sequence in fragments shorter than    2084 bp
  95% of sequence in fragments shorter than    2646 bp

# EciI.HphI (GGCGGA+GGTGA) double digest, 6578 thousand fragments (571273+6007336 sites), 453 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     230 bp
  15% of sequence in fragments shorter than     308 bp
  20% of sequence in fragments shorter than     377 bp
  25% of sequence in fragments shorter than     448 bp
  30% of sequence in fragments shorter than     519 bp
  35% of sequence in fragments shorter than     589 bp
  40% of sequence in fragments shorter than     663 bp
  45% of sequence in fragments shorter than     741 bp
  50% of sequence in fragments shorter than     822 bp
  55% of sequence in fragments shorter than     913 bp
  60% of sequence in fragments shorter than    1015 bp
  65% of sequence in fragments shorter than    1127 bp
  70% of sequence in fragments shorter than    1254 bp
  75% of sequence in fragments shorter than    1391 bp
  80% of sequence in fragments shorter than    1571 bp
  85% of sequence in fragments shorter than    1808 bp
  90% of sequence in fragments shorter than    2133 bp
  95% of sequence in fragments shorter than    2710 bp

# BamHI.BthCI (GGATCC+GCNGC) double digest, 6575 thousand fragments (365999+6209929 sites), 453 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     252 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     432 bp
  25% of sequence in fragments shorter than     528 bp
  30% of sequence in fragments shorter than     627 bp
  35% of sequence in fragments shorter than     726 bp
  40% of sequence in fragments shorter than     833 bp
  45% of sequence in fragments shorter than     940 bp
  50% of sequence in fragments shorter than    1058 bp
  55% of sequence in fragments shorter than    1187 bp
  60% of sequence in fragments shorter than    1326 bp
  65% of sequence in fragments shorter than    1486 bp
  70% of sequence in fragments shorter than    1669 bp
  75% of sequence in fragments shorter than    1883 bp
  80% of sequence in fragments shorter than    2132 bp
  85% of sequence in fragments shorter than    2470 bp
  90% of sequence in fragments shorter than    2934 bp
  95% of sequence in fragments shorter than    3741 bp

# HphI.MfeI (GGTGA+CAATTG) double digest, 6571 thousand fragments (6007336+564303 sites), 453 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     304 bp
  20% of sequence in fragments shorter than     368 bp
  25% of sequence in fragments shorter than     434 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     566 bp
  40% of sequence in fragments shorter than     634 bp
  45% of sequence in fragments shorter than     705 bp
  50% of sequence in fragments shorter than     782 bp
  55% of sequence in fragments shorter than     862 bp
  60% of sequence in fragments shorter than     951 bp
  65% of sequence in fragments shorter than    1049 bp
  70% of sequence in fragments shorter than    1162 bp
  75% of sequence in fragments shorter than    1295 bp
  80% of sequence in fragments shorter than    1444 bp
  85% of sequence in fragments shorter than    1650 bp
  90% of sequence in fragments shorter than    1938 bp
  95% of sequence in fragments shorter than    2413 bp

# BccI.SpeI (CCATC+ACTAGT) double digest, 6570 thousand fragments (6170927+400286 sites), 453 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     377 bp
  25% of sequence in fragments shorter than     444 bp
  30% of sequence in fragments shorter than     511 bp
  35% of sequence in fragments shorter than     583 bp
  40% of sequence in fragments shorter than     654 bp
  45% of sequence in fragments shorter than     730 bp
  50% of sequence in fragments shorter than     811 bp
  55% of sequence in fragments shorter than     899 bp
  60% of sequence in fragments shorter than     995 bp
  65% of sequence in fragments shorter than    1096 bp
  70% of sequence in fragments shorter than    1216 bp
  75% of sequence in fragments shorter than    1357 bp
  80% of sequence in fragments shorter than    1530 bp
  85% of sequence in fragments shorter than    1752 bp
  90% of sequence in fragments shorter than    2069 bp
  95% of sequence in fragments shorter than    2644 bp

# BccI.HpaI (CCATC+GTTAAC) double digest, 6559 thousand fragments (6170927+389418 sites), 454 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     443 bp
  30% of sequence in fragments shorter than     510 bp
  35% of sequence in fragments shorter than     581 bp
  40% of sequence in fragments shorter than     652 bp
  45% of sequence in fragments shorter than     728 bp
  50% of sequence in fragments shorter than     807 bp
  55% of sequence in fragments shorter than     894 bp
  60% of sequence in fragments shorter than     990 bp
  65% of sequence in fragments shorter than    1094 bp
  70% of sequence in fragments shorter than    1214 bp
  75% of sequence in fragments shorter than    1356 bp
  80% of sequence in fragments shorter than    1530 bp
  85% of sequence in fragments shorter than    1754 bp
  90% of sequence in fragments shorter than    2081 bp
  95% of sequence in fragments shorter than    2685 bp

# HphI.SphI (GGTGA+GCATGC) double digest, 6557 thousand fragments (6007336+550952 sites), 454 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     307 bp
  20% of sequence in fragments shorter than     372 bp
  25% of sequence in fragments shorter than     440 bp
  30% of sequence in fragments shorter than     508 bp
  35% of sequence in fragments shorter than     576 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     720 bp
  50% of sequence in fragments shorter than     800 bp
  55% of sequence in fragments shorter than     883 bp
  60% of sequence in fragments shorter than     979 bp
  65% of sequence in fragments shorter than    1085 bp
  70% of sequence in fragments shorter than    1208 bp
  75% of sequence in fragments shorter than    1348 bp
  80% of sequence in fragments shorter than    1504 bp
  85% of sequence in fragments shorter than    1726 bp
  90% of sequence in fragments shorter than    2036 bp
  95% of sequence in fragments shorter than    2575 bp

# AclI.ApoI (AACGTT+RAATTY) double digest, 6554 thousand fragments (172706+6382433 sites), 454 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     232 bp
  15% of sequence in fragments shorter than     303 bp
  20% of sequence in fragments shorter than     373 bp
  25% of sequence in fragments shorter than     445 bp
  30% of sequence in fragments shorter than     521 bp
  35% of sequence in fragments shorter than     597 bp
  40% of sequence in fragments shorter than     678 bp
  45% of sequence in fragments shorter than     762 bp
  50% of sequence in fragments shorter than     855 bp
  55% of sequence in fragments shorter than     951 bp
  60% of sequence in fragments shorter than    1062 bp
  65% of sequence in fragments shorter than    1186 bp
  70% of sequence in fragments shorter than    1327 bp
  75% of sequence in fragments shorter than    1498 bp
  80% of sequence in fragments shorter than    1721 bp
  85% of sequence in fragments shorter than    2019 bp
  90% of sequence in fragments shorter than    2478 bp
  95% of sequence in fragments shorter than    3443 bp

# CviQI.FauI (GTAC+CCCGC) double digest, 6554 thousand fragments (5115078+1439772 sites), 454 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     243 bp
  15% of sequence in fragments shorter than     319 bp
  20% of sequence in fragments shorter than     388 bp
  25% of sequence in fragments shorter than     457 bp
  30% of sequence in fragments shorter than     524 bp
  35% of sequence in fragments shorter than     592 bp
  40% of sequence in fragments shorter than     665 bp
  45% of sequence in fragments shorter than     738 bp
  50% of sequence in fragments shorter than     815 bp
  55% of sequence in fragments shorter than     898 bp
  60% of sequence in fragments shorter than     991 bp
  65% of sequence in fragments shorter than    1092 bp
  70% of sequence in fragments shorter than    1205 bp
  75% of sequence in fragments shorter than    1332 bp
  80% of sequence in fragments shorter than    1478 bp
  85% of sequence in fragments shorter than    1677 bp
  90% of sequence in fragments shorter than    1963 bp
  95% of sequence in fragments shorter than    2449 bp

# BspMI.CviQI (ACCTGC+GTAC) double digest, 6552 thousand fragments (1438129+5115078 sites), 454 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     244 bp
  15% of sequence in fragments shorter than     319 bp
  20% of sequence in fragments shorter than     385 bp
  25% of sequence in fragments shorter than     453 bp
  30% of sequence in fragments shorter than     518 bp
  35% of sequence in fragments shorter than     584 bp
  40% of sequence in fragments shorter than     653 bp
  45% of sequence in fragments shorter than     722 bp
  50% of sequence in fragments shorter than     797 bp
  55% of sequence in fragments shorter than     875 bp
  60% of sequence in fragments shorter than     964 bp
  65% of sequence in fragments shorter than    1061 bp
  70% of sequence in fragments shorter than    1172 bp
  75% of sequence in fragments shorter than    1293 bp
  80% of sequence in fragments shorter than    1426 bp
  85% of sequence in fragments shorter than    1617 bp
  90% of sequence in fragments shorter than    1884 bp
  95% of sequence in fragments shorter than    2326 bp

# HphI.ScaI (GGTGA+AGTACT) double digest, 6550 thousand fragments (6007336+543793 sites), 454 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     306 bp
  20% of sequence in fragments shorter than     370 bp
  25% of sequence in fragments shorter than     437 bp
  30% of sequence in fragments shorter than     504 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     638 bp
  45% of sequence in fragments shorter than     710 bp
  50% of sequence in fragments shorter than     788 bp
  55% of sequence in fragments shorter than     866 bp
  60% of sequence in fragments shorter than     959 bp
  65% of sequence in fragments shorter than    1062 bp
  70% of sequence in fragments shorter than    1179 bp
  75% of sequence in fragments shorter than    1313 bp
  80% of sequence in fragments shorter than    1462 bp
  85% of sequence in fragments shorter than    1671 bp
  90% of sequence in fragments shorter than    1971 bp
  95% of sequence in fragments shorter than    2473 bp

# BccI.SapI (CCATC+GCTCTTC) double digest, 6547 thousand fragments (6170927+377161 sites), 455 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     377 bp
  25% of sequence in fragments shorter than     445 bp
  30% of sequence in fragments shorter than     514 bp
  35% of sequence in fragments shorter than     587 bp
  40% of sequence in fragments shorter than     659 bp
  45% of sequence in fragments shorter than     737 bp
  50% of sequence in fragments shorter than     820 bp
  55% of sequence in fragments shorter than     909 bp
  60% of sequence in fragments shorter than    1005 bp
  65% of sequence in fragments shorter than    1113 bp
  70% of sequence in fragments shorter than    1238 bp
  75% of sequence in fragments shorter than    1385 bp
  80% of sequence in fragments shorter than    1566 bp
  85% of sequence in fragments shorter than    1800 bp
  90% of sequence in fragments shorter than    2144 bp
  95% of sequence in fragments shorter than    2777 bp

# BccI.SmaI (CCATC+CCCGGG) double digest, 6547 thousand fragments (6170927+376940 sites), 455 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     236 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     448 bp
  30% of sequence in fragments shorter than     518 bp
  35% of sequence in fragments shorter than     592 bp
  40% of sequence in fragments shorter than     666 bp
  45% of sequence in fragments shorter than     746 bp
  50% of sequence in fragments shorter than     831 bp
  55% of sequence in fragments shorter than     923 bp
  60% of sequence in fragments shorter than    1026 bp
  65% of sequence in fragments shorter than    1134 bp
  70% of sequence in fragments shorter than    1262 bp
  75% of sequence in fragments shorter than    1415 bp
  80% of sequence in fragments shorter than    1603 bp
  85% of sequence in fragments shorter than    1847 bp
  90% of sequence in fragments shorter than    2208 bp
  95% of sequence in fragments shorter than    2869 bp

# BstEII.BthCI (GGTNACC+GCNGC) double digest, 6545 thousand fragments (335865+6209929 sites), 455 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     254 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     437 bp
  25% of sequence in fragments shorter than     533 bp
  30% of sequence in fragments shorter than     631 bp
  35% of sequence in fragments shorter than     731 bp
  40% of sequence in fragments shorter than     836 bp
  45% of sequence in fragments shorter than     945 bp
  50% of sequence in fragments shorter than    1063 bp
  55% of sequence in fragments shorter than    1194 bp
  60% of sequence in fragments shorter than    1337 bp
  65% of sequence in fragments shorter than    1497 bp
  70% of sequence in fragments shorter than    1674 bp
  75% of sequence in fragments shorter than    1889 bp
  80% of sequence in fragments shorter than    2133 bp
  85% of sequence in fragments shorter than    2468 bp
  90% of sequence in fragments shorter than    2933 bp
  95% of sequence in fragments shorter than    3735 bp

# ApoI.PacI (RAATTY+TTAATTAA) double digest, 6541 thousand fragments (6382433+159455 sites), 455 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     375 bp
  25% of sequence in fragments shorter than     448 bp
  30% of sequence in fragments shorter than     524 bp
  35% of sequence in fragments shorter than     601 bp
  40% of sequence in fragments shorter than     683 bp
  45% of sequence in fragments shorter than     768 bp
  50% of sequence in fragments shorter than     861 bp
  55% of sequence in fragments shorter than     958 bp
  60% of sequence in fragments shorter than    1071 bp
  65% of sequence in fragments shorter than    1200 bp
  70% of sequence in fragments shorter than    1339 bp
  75% of sequence in fragments shorter than    1513 bp
  80% of sequence in fragments shorter than    1738 bp
  85% of sequence in fragments shorter than    2047 bp
  90% of sequence in fragments shorter than    2520 bp
  95% of sequence in fragments shorter than    3519 bp

# ApoI.Hpy99I (RAATTY+CGWCG) double digest, 6539 thousand fragments (6382433+157917 sites), 455 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     375 bp
  25% of sequence in fragments shorter than     447 bp
  30% of sequence in fragments shorter than     522 bp
  35% of sequence in fragments shorter than     598 bp
  40% of sequence in fragments shorter than     677 bp
  45% of sequence in fragments shorter than     760 bp
  50% of sequence in fragments shorter than     850 bp
  55% of sequence in fragments shorter than     943 bp
  60% of sequence in fragments shorter than    1051 bp
  65% of sequence in fragments shorter than    1172 bp
  70% of sequence in fragments shorter than    1309 bp
  75% of sequence in fragments shorter than    1464 bp
  80% of sequence in fragments shorter than    1673 bp
  85% of sequence in fragments shorter than    1943 bp
  90% of sequence in fragments shorter than    2347 bp
  95% of sequence in fragments shorter than    3126 bp

# BamHI.BccI (GGATCC+CCATC) double digest, 6536 thousand fragments (365999+6170927 sites), 455 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     239 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     378 bp
  25% of sequence in fragments shorter than     448 bp
  30% of sequence in fragments shorter than     516 bp
  35% of sequence in fragments shorter than     589 bp
  40% of sequence in fragments shorter than     662 bp
  45% of sequence in fragments shorter than     740 bp
  50% of sequence in fragments shorter than     824 bp
  55% of sequence in fragments shorter than     915 bp
  60% of sequence in fragments shorter than    1015 bp
  65% of sequence in fragments shorter than    1121 bp
  70% of sequence in fragments shorter than    1247 bp
  75% of sequence in fragments shorter than    1395 bp
  80% of sequence in fragments shorter than    1578 bp
  85% of sequence in fragments shorter than    1815 bp
  90% of sequence in fragments shorter than    2161 bp
  95% of sequence in fragments shorter than    2804 bp

# HgiCI.TfiI (GGYRCC+GAWTC) double digest, 6534 thousand fragments (1550877+4984362 sites), 456 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     312 bp
  20% of sequence in fragments shorter than     363 bp
  25% of sequence in fragments shorter than     429 bp
  30% of sequence in fragments shorter than     494 bp
  35% of sequence in fragments shorter than     563 bp
  40% of sequence in fragments shorter than     633 bp
  45% of sequence in fragments shorter than     701 bp
  50% of sequence in fragments shorter than     776 bp
  55% of sequence in fragments shorter than     855 bp
  60% of sequence in fragments shorter than     942 bp
  65% of sequence in fragments shorter than    1037 bp
  70% of sequence in fragments shorter than    1146 bp
  75% of sequence in fragments shorter than    1272 bp
  80% of sequence in fragments shorter than    1424 bp
  85% of sequence in fragments shorter than    1615 bp
  90% of sequence in fragments shorter than    1879 bp
  95% of sequence in fragments shorter than    2322 bp

# BsmI.TfiI (GAATGC+GAWTC) double digest, 6533 thousand fragments (1549350+4984362 sites), 456 bp average fragment length
   5% of sequence in fragments shorter than     148 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     375 bp
  25% of sequence in fragments shorter than     447 bp
  30% of sequence in fragments shorter than     518 bp
  35% of sequence in fragments shorter than     591 bp
  40% of sequence in fragments shorter than     665 bp
  45% of sequence in fragments shorter than     741 bp
  50% of sequence in fragments shorter than     820 bp
  55% of sequence in fragments shorter than     906 bp
  60% of sequence in fragments shorter than    1001 bp
  65% of sequence in fragments shorter than    1105 bp
  70% of sequence in fragments shorter than    1223 bp
  75% of sequence in fragments shorter than    1357 bp
  80% of sequence in fragments shorter than    1519 bp
  85% of sequence in fragments shorter than    1724 bp
  90% of sequence in fragments shorter than    2005 bp
  95% of sequence in fragments shorter than    2485 bp

# AciI.HpaII (CCGC+CCGG) double digest, 6523 thousand fragments (4206130+2317722 sites), 456 bp average fragment length
   5% of sequence in fragments shorter than     141 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     401 bp
  20% of sequence in fragments shorter than     539 bp
  25% of sequence in fragments shorter than     682 bp
  30% of sequence in fragments shorter than     832 bp
  35% of sequence in fragments shorter than     986 bp
  40% of sequence in fragments shorter than    1151 bp
  45% of sequence in fragments shorter than    1331 bp
  50% of sequence in fragments shorter than    1530 bp
  55% of sequence in fragments shorter than    1754 bp
  60% of sequence in fragments shorter than    2006 bp
  65% of sequence in fragments shorter than    2289 bp
  70% of sequence in fragments shorter than    2621 bp
  75% of sequence in fragments shorter than    3015 bp
  80% of sequence in fragments shorter than    3498 bp
  85% of sequence in fragments shorter than    4127 bp
  90% of sequence in fragments shorter than    5017 bp
  95% of sequence in fragments shorter than    6577 bp

# FokI.PstI (GGATG+CTGCAG) double digest, 6522 thousand fragments (5201116+1321469 sites), 456 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     314 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     454 bp
  30% of sequence in fragments shorter than     526 bp
  35% of sequence in fragments shorter than     601 bp
  40% of sequence in fragments shorter than     677 bp
  45% of sequence in fragments shorter than     755 bp
  50% of sequence in fragments shorter than     840 bp
  55% of sequence in fragments shorter than     929 bp
  60% of sequence in fragments shorter than    1030 bp
  65% of sequence in fragments shorter than    1139 bp
  70% of sequence in fragments shorter than    1265 bp
  75% of sequence in fragments shorter than    1405 bp
  80% of sequence in fragments shorter than    1582 bp
  85% of sequence in fragments shorter than    1813 bp
  90% of sequence in fragments shorter than    2133 bp
  95% of sequence in fragments shorter than    2715 bp

# BbvI.BscGI (GCAGC+CCCGT) double digest, 6520 thousand fragments (5290051+1231070 sites), 457 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     243 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     395 bp
  25% of sequence in fragments shorter than     482 bp
  30% of sequence in fragments shorter than     572 bp
  35% of sequence in fragments shorter than     665 bp
  40% of sequence in fragments shorter than     762 bp
  45% of sequence in fragments shorter than     866 bp
  50% of sequence in fragments shorter than     978 bp
  55% of sequence in fragments shorter than    1101 bp
  60% of sequence in fragments shorter than    1238 bp
  65% of sequence in fragments shorter than    1389 bp
  70% of sequence in fragments shorter than    1563 bp
  75% of sequence in fragments shorter than    1753 bp
  80% of sequence in fragments shorter than    1993 bp
  85% of sequence in fragments shorter than    2298 bp
  90% of sequence in fragments shorter than    2740 bp
  95% of sequence in fragments shorter than    3485 bp

# BglII.BsrI (AGATCT+ACTGG) double digest, 6515 thousand fragments (774732+5741310 sites), 457 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     308 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     445 bp
  30% of sequence in fragments shorter than     511 bp
  35% of sequence in fragments shorter than     581 bp
  40% of sequence in fragments shorter than     651 bp
  45% of sequence in fragments shorter than     718 bp
  50% of sequence in fragments shorter than     797 bp
  55% of sequence in fragments shorter than     880 bp
  60% of sequence in fragments shorter than     974 bp
  65% of sequence in fragments shorter than    1075 bp
  70% of sequence in fragments shorter than    1193 bp
  75% of sequence in fragments shorter than    1330 bp
  80% of sequence in fragments shorter than    1492 bp
  85% of sequence in fragments shorter than    1706 bp
  90% of sequence in fragments shorter than    2004 bp
  95% of sequence in fragments shorter than    2541 bp

# ApoI.SnaBI (RAATTY+TACGTA) double digest, 6514 thousand fragments (6382433+132299 sites), 457 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     234 bp
  15% of sequence in fragments shorter than     305 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     449 bp
  30% of sequence in fragments shorter than     525 bp
  35% of sequence in fragments shorter than     601 bp
  40% of sequence in fragments shorter than     683 bp
  45% of sequence in fragments shorter than     767 bp
  50% of sequence in fragments shorter than     860 bp
  55% of sequence in fragments shorter than     957 bp
  60% of sequence in fragments shorter than    1069 bp
  65% of sequence in fragments shorter than    1197 bp
  70% of sequence in fragments shorter than    1336 bp
  75% of sequence in fragments shorter than    1506 bp
  80% of sequence in fragments shorter than    1728 bp
  85% of sequence in fragments shorter than    2030 bp
  90% of sequence in fragments shorter than    2491 bp
  95% of sequence in fragments shorter than    3441 bp

# ApoI.NaeI (RAATTY+GCCGGC) double digest, 6507 thousand fragments (6382433+125692 sites), 457 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     236 bp
  15% of sequence in fragments shorter than     307 bp
  20% of sequence in fragments shorter than     377 bp
  25% of sequence in fragments shorter than     449 bp
  30% of sequence in fragments shorter than     525 bp
  35% of sequence in fragments shorter than     601 bp
  40% of sequence in fragments shorter than     681 bp
  45% of sequence in fragments shorter than     764 bp
  50% of sequence in fragments shorter than     854 bp
  55% of sequence in fragments shorter than     949 bp
  60% of sequence in fragments shorter than    1057 bp
  65% of sequence in fragments shorter than    1179 bp
  70% of sequence in fragments shorter than    1312 bp
  75% of sequence in fragments shorter than    1471 bp
  80% of sequence in fragments shorter than    1678 bp
  85% of sequence in fragments shorter than    1949 bp
  90% of sequence in fragments shorter than    2350 bp
  95% of sequence in fragments shorter than    3125 bp

# BthCI.SexAI (GCNGC+ACCWGGT) double digest, 6507 thousand fragments (6209929+297900 sites), 457 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     255 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     439 bp
  25% of sequence in fragments shorter than     535 bp
  30% of sequence in fragments shorter than     633 bp
  35% of sequence in fragments shorter than     734 bp
  40% of sequence in fragments shorter than     839 bp
  45% of sequence in fragments shorter than     948 bp
  50% of sequence in fragments shorter than    1067 bp
  55% of sequence in fragments shorter than    1199 bp
  60% of sequence in fragments shorter than    1342 bp
  65% of sequence in fragments shorter than    1502 bp
  70% of sequence in fragments shorter than    1680 bp
  75% of sequence in fragments shorter than    1895 bp
  80% of sequence in fragments shorter than    2143 bp
  85% of sequence in fragments shorter than    2481 bp
  90% of sequence in fragments shorter than    2941 bp
  95% of sequence in fragments shorter than    3755 bp

# BccI.BstEII (CCATC+GGTNACC) double digest, 6506 thousand fragments (6170927+335865 sites), 458 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     312 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     450 bp
  30% of sequence in fragments shorter than     519 bp
  35% of sequence in fragments shorter than     592 bp
  40% of sequence in fragments shorter than     666 bp
  45% of sequence in fragments shorter than     745 bp
  50% of sequence in fragments shorter than     829 bp
  55% of sequence in fragments shorter than     919 bp
  60% of sequence in fragments shorter than    1020 bp
  65% of sequence in fragments shorter than    1126 bp
  70% of sequence in fragments shorter than    1252 bp
  75% of sequence in fragments shorter than    1400 bp
  80% of sequence in fragments shorter than    1582 bp
  85% of sequence in fragments shorter than    1819 bp
  90% of sequence in fragments shorter than    2169 bp
  95% of sequence in fragments shorter than    2811 bp

# ApoI.XhoI (RAATTY+CTCGAG) double digest, 6503 thousand fragments (6382433+121308 sites), 458 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     306 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     448 bp
  30% of sequence in fragments shorter than     524 bp
  35% of sequence in fragments shorter than     600 bp
  40% of sequence in fragments shorter than     682 bp
  45% of sequence in fragments shorter than     766 bp
  50% of sequence in fragments shorter than     858 bp
  55% of sequence in fragments shorter than     953 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1189 bp
  70% of sequence in fragments shorter than    1324 bp
  75% of sequence in fragments shorter than    1490 bp
  80% of sequence in fragments shorter than    1707 bp
  85% of sequence in fragments shorter than    1994 bp
  90% of sequence in fragments shorter than    2426 bp
  95% of sequence in fragments shorter than    3302 bp

# ApaLI.HphI (GTGCAC+GGTGA) double digest, 6503 thousand fragments (496255+6007336 sites), 458 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     236 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     375 bp
  25% of sequence in fragments shorter than     444 bp
  30% of sequence in fragments shorter than     512 bp
  35% of sequence in fragments shorter than     580 bp
  40% of sequence in fragments shorter than     650 bp
  45% of sequence in fragments shorter than     723 bp
  50% of sequence in fragments shorter than     802 bp
  55% of sequence in fragments shorter than     885 bp
  60% of sequence in fragments shorter than     982 bp
  65% of sequence in fragments shorter than    1088 bp
  70% of sequence in fragments shorter than    1211 bp
  75% of sequence in fragments shorter than    1351 bp
  80% of sequence in fragments shorter than    1507 bp
  85% of sequence in fragments shorter than    1729 bp
  90% of sequence in fragments shorter than    2033 bp
  95% of sequence in fragments shorter than    2562 bp

# BsrI.NcoI (ACTGG+CCATGG) double digest, 6500 thousand fragments (5741310+759594 sites), 458 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     310 bp
  20% of sequence in fragments shorter than     380 bp
  25% of sequence in fragments shorter than     451 bp
  30% of sequence in fragments shorter than     518 bp
  35% of sequence in fragments shorter than     590 bp
  40% of sequence in fragments shorter than     663 bp
  45% of sequence in fragments shorter than     732 bp
  50% of sequence in fragments shorter than     815 bp
  55% of sequence in fragments shorter than     899 bp
  60% of sequence in fragments shorter than     996 bp
  65% of sequence in fragments shorter than    1102 bp
  70% of sequence in fragments shorter than    1224 bp
  75% of sequence in fragments shorter than    1369 bp
  80% of sequence in fragments shorter than    1533 bp
  85% of sequence in fragments shorter than    1751 bp
  90% of sequence in fragments shorter than    2060 bp
  95% of sequence in fragments shorter than    2613 bp

# BthCI.KpnI (GCNGC+GGTACC) double digest, 6497 thousand fragments (6209929+287974 sites), 458 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     344 bp
  20% of sequence in fragments shorter than     440 bp
  25% of sequence in fragments shorter than     538 bp
  30% of sequence in fragments shorter than     633 bp
  35% of sequence in fragments shorter than     734 bp
  40% of sequence in fragments shorter than     839 bp
  45% of sequence in fragments shorter than     948 bp
  50% of sequence in fragments shorter than    1059 bp
  55% of sequence in fragments shorter than    1193 bp
  60% of sequence in fragments shorter than    1334 bp
  65% of sequence in fragments shorter than    1494 bp
  70% of sequence in fragments shorter than    1670 bp
  75% of sequence in fragments shorter than    1882 bp
  80% of sequence in fragments shorter than    2142 bp
  85% of sequence in fragments shorter than    2480 bp
  90% of sequence in fragments shorter than    2940 bp
  95% of sequence in fragments shorter than    3759 bp

# BthCI.NheI (GCNGC+GCTAGC) double digest, 6496 thousand fragments (6209929+287384 sites), 458 bp average fragment length
   5% of sequence in fragments shorter than     152 bp
  10% of sequence in fragments shorter than     257 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     442 bp
  25% of sequence in fragments shorter than     536 bp
  30% of sequence in fragments shorter than     635 bp
  35% of sequence in fragments shorter than     734 bp
  40% of sequence in fragments shorter than     838 bp
  45% of sequence in fragments shorter than     947 bp
  50% of sequence in fragments shorter than    1065 bp
  55% of sequence in fragments shorter than    1188 bp
  60% of sequence in fragments shorter than    1330 bp
  65% of sequence in fragments shorter than    1483 bp
  70% of sequence in fragments shorter than    1665 bp
  75% of sequence in fragments shorter than    1879 bp
  80% of sequence in fragments shorter than    2139 bp
  85% of sequence in fragments shorter than    2473 bp
  90% of sequence in fragments shorter than    2936 bp
  95% of sequence in fragments shorter than    3748 bp

# BclI.BsrI (TGATCA+ACTGG) double digest, 6479 thousand fragments (738785+5741310 sites), 459 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     380 bp
  25% of sequence in fragments shorter than     449 bp
  30% of sequence in fragments shorter than     515 bp
  35% of sequence in fragments shorter than     585 bp
  40% of sequence in fragments shorter than     656 bp
  45% of sequence in fragments shorter than     723 bp
  50% of sequence in fragments shorter than     802 bp
  55% of sequence in fragments shorter than     882 bp
  60% of sequence in fragments shorter than     975 bp
  65% of sequence in fragments shorter than    1076 bp
  70% of sequence in fragments shorter than    1192 bp
  75% of sequence in fragments shorter than    1328 bp
  80% of sequence in fragments shorter than    1488 bp
  85% of sequence in fragments shorter than    1699 bp
  90% of sequence in fragments shorter than    1993 bp
  95% of sequence in fragments shorter than    2514 bp

# BsrI.FnuDII (ACTGG+CGCG) double digest, 6474 thousand fragments (5741310+733940 sites), 460 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     239 bp
  15% of sequence in fragments shorter than     315 bp
  20% of sequence in fragments shorter than     388 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     529 bp
  35% of sequence in fragments shorter than     603 bp
  40% of sequence in fragments shorter than     677 bp
  45% of sequence in fragments shorter than     748 bp
  50% of sequence in fragments shorter than     831 bp
  55% of sequence in fragments shorter than     919 bp
  60% of sequence in fragments shorter than    1019 bp
  65% of sequence in fragments shorter than    1127 bp
  70% of sequence in fragments shorter than    1251 bp
  75% of sequence in fragments shorter than    1397 bp
  80% of sequence in fragments shorter than    1571 bp
  85% of sequence in fragments shorter than    1795 bp
  90% of sequence in fragments shorter than    2112 bp
  95% of sequence in fragments shorter than    2693 bp

# AflIII.TfiI (ACRYGT+GAWTC) double digest, 6469 thousand fragments (1485394+4984362 sites), 460 bp average fragment length
   5% of sequence in fragments shorter than     164 bp
  10% of sequence in fragments shorter than     243 bp
  15% of sequence in fragments shorter than     317 bp
  20% of sequence in fragments shorter than     369 bp
  25% of sequence in fragments shorter than     433 bp
  30% of sequence in fragments shorter than     500 bp
  35% of sequence in fragments shorter than     570 bp
  40% of sequence in fragments shorter than     642 bp
  45% of sequence in fragments shorter than     712 bp
  50% of sequence in fragments shorter than     789 bp
  55% of sequence in fragments shorter than     869 bp
  60% of sequence in fragments shorter than     960 bp
  65% of sequence in fragments shorter than    1059 bp
  70% of sequence in fragments shorter than    1170 bp
  75% of sequence in fragments shorter than    1300 bp
  80% of sequence in fragments shorter than    1456 bp
  85% of sequence in fragments shorter than    1655 bp
  90% of sequence in fragments shorter than    1923 bp
  95% of sequence in fragments shorter than    2376 bp

# ApoI.ClaI (RAATTY+ATCGAT) double digest, 6468 thousand fragments (6382433+86477 sites), 460 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     307 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     452 bp
  30% of sequence in fragments shorter than     528 bp
  35% of sequence in fragments shorter than     606 bp
  40% of sequence in fragments shorter than     687 bp
  45% of sequence in fragments shorter than     773 bp
  50% of sequence in fragments shorter than     866 bp
  55% of sequence in fragments shorter than     964 bp
  60% of sequence in fragments shorter than    1076 bp
  65% of sequence in fragments shorter than    1205 bp
  70% of sequence in fragments shorter than    1346 bp
  75% of sequence in fragments shorter than    1519 bp
  80% of sequence in fragments shorter than    1743 bp
  85% of sequence in fragments shorter than    2050 bp
  90% of sequence in fragments shorter than    2517 bp
  95% of sequence in fragments shorter than    3495 bp

# BccI.SexAI (CCATC+ACCWGGT) double digest, 6468 thousand fragments (6170927+297900 sites), 460 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     313 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     451 bp
  30% of sequence in fragments shorter than     520 bp
  35% of sequence in fragments shorter than     594 bp
  40% of sequence in fragments shorter than     667 bp
  45% of sequence in fragments shorter than     746 bp
  50% of sequence in fragments shorter than     831 bp
  55% of sequence in fragments shorter than     921 bp
  60% of sequence in fragments shorter than    1022 bp
  65% of sequence in fragments shorter than    1128 bp
  70% of sequence in fragments shorter than    1254 bp
  75% of sequence in fragments shorter than    1403 bp
  80% of sequence in fragments shorter than    1586 bp
  85% of sequence in fragments shorter than    1823 bp
  90% of sequence in fragments shorter than    2170 bp
  95% of sequence in fragments shorter than    2808 bp

# ApaI.HphI (GGGCCC+GGTGA) double digest, 6467 thousand fragments (460339+6007336 sites), 460 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     235 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     446 bp
  30% of sequence in fragments shorter than     515 bp
  35% of sequence in fragments shorter than     585 bp
  40% of sequence in fragments shorter than     658 bp
  45% of sequence in fragments shorter than     734 bp
  50% of sequence in fragments shorter than     814 bp
  55% of sequence in fragments shorter than     903 bp
  60% of sequence in fragments shorter than    1004 bp
  65% of sequence in fragments shorter than    1115 bp
  70% of sequence in fragments shorter than    1242 bp
  75% of sequence in fragments shorter than    1380 bp
  80% of sequence in fragments shorter than    1555 bp
  85% of sequence in fragments shorter than    1791 bp
  90% of sequence in fragments shorter than    2116 bp
  95% of sequence in fragments shorter than    2694 bp

# BccI.KpnI (CCATC+GGTACC) double digest, 6458 thousand fragments (6170927+287974 sites), 461 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     239 bp
  15% of sequence in fragments shorter than     312 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     453 bp
  30% of sequence in fragments shorter than     524 bp
  35% of sequence in fragments shorter than     599 bp
  40% of sequence in fragments shorter than     673 bp
  45% of sequence in fragments shorter than     752 bp
  50% of sequence in fragments shorter than     838 bp
  55% of sequence in fragments shorter than     929 bp
  60% of sequence in fragments shorter than    1031 bp
  65% of sequence in fragments shorter than    1138 bp
  70% of sequence in fragments shorter than    1264 bp
  75% of sequence in fragments shorter than    1414 bp
  80% of sequence in fragments shorter than    1599 bp
  85% of sequence in fragments shorter than    1839 bp
  90% of sequence in fragments shorter than    2191 bp
  95% of sequence in fragments shorter than    2840 bp

# BccI.NheI (CCATC+GCTAGC) double digest, 6457 thousand fragments (6170927+287384 sites), 461 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     314 bp
  20% of sequence in fragments shorter than     382 bp
  25% of sequence in fragments shorter than     452 bp
  30% of sequence in fragments shorter than     522 bp
  35% of sequence in fragments shorter than     596 bp
  40% of sequence in fragments shorter than     669 bp
  45% of sequence in fragments shorter than     748 bp
  50% of sequence in fragments shorter than     833 bp
  55% of sequence in fragments shorter than     920 bp
  60% of sequence in fragments shorter than    1021 bp
  65% of sequence in fragments shorter than    1132 bp
  70% of sequence in fragments shorter than    1258 bp
  75% of sequence in fragments shorter than    1409 bp
  80% of sequence in fragments shorter than    1593 bp
  85% of sequence in fragments shorter than    1831 bp
  90% of sequence in fragments shorter than    2172 bp
  95% of sequence in fragments shorter than    2799 bp

# BsaAI.BsrI (YACGTR+ACTGG) double digest, 6454 thousand fragments (713801+5741310 sites), 461 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     312 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     452 bp
  30% of sequence in fragments shorter than     519 bp
  35% of sequence in fragments shorter than     590 bp
  40% of sequence in fragments shorter than     662 bp
  45% of sequence in fragments shorter than     730 bp
  50% of sequence in fragments shorter than     811 bp
  55% of sequence in fragments shorter than     894 bp
  60% of sequence in fragments shorter than     988 bp
  65% of sequence in fragments shorter than    1092 bp
  70% of sequence in fragments shorter than    1211 bp
  75% of sequence in fragments shorter than    1349 bp
  80% of sequence in fragments shorter than    1511 bp
  85% of sequence in fragments shorter than    1724 bp
  90% of sequence in fragments shorter than    2023 bp
  95% of sequence in fragments shorter than    2559 bp

# EcoRV.HphI (GATATC+GGTGA) double digest, 6453 thousand fragments (446473+6007336 sites), 461 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     239 bp
  15% of sequence in fragments shorter than     312 bp
  20% of sequence in fragments shorter than     378 bp
  25% of sequence in fragments shorter than     446 bp
  30% of sequence in fragments shorter than     514 bp
  35% of sequence in fragments shorter than     581 bp
  40% of sequence in fragments shorter than     651 bp
  45% of sequence in fragments shorter than     722 bp
  50% of sequence in fragments shorter than     801 bp
  55% of sequence in fragments shorter than     882 bp
  60% of sequence in fragments shorter than     977 bp
  65% of sequence in fragments shorter than    1081 bp
  70% of sequence in fragments shorter than    1201 bp
  75% of sequence in fragments shorter than    1336 bp
  80% of sequence in fragments shorter than    1492 bp
  85% of sequence in fragments shorter than    1705 bp
  90% of sequence in fragments shorter than    2005 bp
  95% of sequence in fragments shorter than    2512 bp

# AatII.ApoI (GACGTC+RAATTY) double digest, 6453 thousand fragments (71089+6382433 sites), 461 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     308 bp
  20% of sequence in fragments shorter than     380 bp
  25% of sequence in fragments shorter than     453 bp
  30% of sequence in fragments shorter than     529 bp
  35% of sequence in fragments shorter than     607 bp
  40% of sequence in fragments shorter than     687 bp
  45% of sequence in fragments shorter than     773 bp
  50% of sequence in fragments shorter than     866 bp
  55% of sequence in fragments shorter than     963 bp
  60% of sequence in fragments shorter than    1075 bp
  65% of sequence in fragments shorter than    1202 bp
  70% of sequence in fragments shorter than    1340 bp
  75% of sequence in fragments shorter than    1510 bp
  80% of sequence in fragments shorter than    1732 bp
  85% of sequence in fragments shorter than    2028 bp
  90% of sequence in fragments shorter than    2476 bp
  95% of sequence in fragments shorter than    3393 bp

# ApoI.SacII (RAATTY+CCGCGG) double digest, 6448 thousand fragments (6382433+66901 sites), 462 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     454 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     607 bp
  40% of sequence in fragments shorter than     688 bp
  45% of sequence in fragments shorter than     774 bp
  50% of sequence in fragments shorter than     866 bp
  55% of sequence in fragments shorter than     962 bp
  60% of sequence in fragments shorter than    1073 bp
  65% of sequence in fragments shorter than    1200 bp
  70% of sequence in fragments shorter than    1336 bp
  75% of sequence in fragments shorter than    1504 bp
  80% of sequence in fragments shorter than    1722 bp
  85% of sequence in fragments shorter than    2009 bp
  90% of sequence in fragments shorter than    2445 bp
  95% of sequence in fragments shorter than    3330 bp

# BceAI.BsrI (ACGGC+ACTGG) double digest, 6445 thousand fragments (705178+5741310 sites), 462 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     382 bp
  25% of sequence in fragments shorter than     453 bp
  30% of sequence in fragments shorter than     521 bp
  35% of sequence in fragments shorter than     594 bp
  40% of sequence in fragments shorter than     668 bp
  45% of sequence in fragments shorter than     737 bp
  50% of sequence in fragments shorter than     820 bp
  55% of sequence in fragments shorter than     906 bp
  60% of sequence in fragments shorter than    1004 bp
  65% of sequence in fragments shorter than    1111 bp
  70% of sequence in fragments shorter than    1234 bp
  75% of sequence in fragments shorter than    1378 bp
  80% of sequence in fragments shorter than    1550 bp
  85% of sequence in fragments shorter than    1771 bp
  90% of sequence in fragments shorter than    2083 bp
  95% of sequence in fragments shorter than    2646 bp

# BsrBI.BthCI (CCGCTC+GCNGC) double digest, 6444 thousand fragments (235523+6209929 sites), 462 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     353 bp
  20% of sequence in fragments shorter than     452 bp
  25% of sequence in fragments shorter than     552 bp
  30% of sequence in fragments shorter than     655 bp
  35% of sequence in fragments shorter than     761 bp
  40% of sequence in fragments shorter than     866 bp
  45% of sequence in fragments shorter than     982 bp
  50% of sequence in fragments shorter than    1104 bp
  55% of sequence in fragments shorter than    1239 bp
  60% of sequence in fragments shorter than    1386 bp
  65% of sequence in fragments shorter than    1556 bp
  70% of sequence in fragments shorter than    1740 bp
  75% of sequence in fragments shorter than    1962 bp
  80% of sequence in fragments shorter than    2228 bp
  85% of sequence in fragments shorter than    2579 bp
  90% of sequence in fragments shorter than    3064 bp
  95% of sequence in fragments shorter than    3907 bp

# ApoI.PspXI (RAATTY+VCTCGAGB) double digest, 6441 thousand fragments (6382433+59193 sites), 462 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     454 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     608 bp
  40% of sequence in fragments shorter than     689 bp
  45% of sequence in fragments shorter than     775 bp
  50% of sequence in fragments shorter than     868 bp
  55% of sequence in fragments shorter than     965 bp
  60% of sequence in fragments shorter than    1077 bp
  65% of sequence in fragments shorter than    1205 bp
  70% of sequence in fragments shorter than    1343 bp
  75% of sequence in fragments shorter than    1514 bp
  80% of sequence in fragments shorter than    1734 bp
  85% of sequence in fragments shorter than    2032 bp
  90% of sequence in fragments shorter than    2481 bp
  95% of sequence in fragments shorter than    3403 bp

# BthCI.KasI (GCNGC+GGCGCC) double digest, 6439 thousand fragments (6209929+230381 sites), 462 bp average fragment length
   5% of sequence in fragments shorter than     154 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     350 bp
  20% of sequence in fragments shorter than     447 bp
  25% of sequence in fragments shorter than     544 bp
  30% of sequence in fragments shorter than     644 bp
  35% of sequence in fragments shorter than     748 bp
  40% of sequence in fragments shorter than     855 bp
  45% of sequence in fragments shorter than     966 bp
  50% of sequence in fragments shorter than    1085 bp
  55% of sequence in fragments shorter than    1220 bp
  60% of sequence in fragments shorter than    1368 bp
  65% of sequence in fragments shorter than    1531 bp
  70% of sequence in fragments shorter than    1713 bp
  75% of sequence in fragments shorter than    1932 bp
  80% of sequence in fragments shorter than    2186 bp
  85% of sequence in fragments shorter than    2533 bp
  90% of sequence in fragments shorter than    3006 bp
  95% of sequence in fragments shorter than    3835 bp

# BciVI.BsrI (GTATCC+ACTGG) double digest, 6436 thousand fragments (696093+5741310 sites), 462 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     312 bp
  20% of sequence in fragments shorter than     382 bp
  25% of sequence in fragments shorter than     451 bp
  30% of sequence in fragments shorter than     516 bp
  35% of sequence in fragments shorter than     587 bp
  40% of sequence in fragments shorter than     660 bp
  45% of sequence in fragments shorter than     732 bp
  50% of sequence in fragments shorter than     814 bp
  55% of sequence in fragments shorter than     898 bp
  60% of sequence in fragments shorter than     994 bp
  65% of sequence in fragments shorter than    1098 bp
  70% of sequence in fragments shorter than    1217 bp
  75% of sequence in fragments shorter than    1356 bp
  80% of sequence in fragments shorter than    1521 bp
  85% of sequence in fragments shorter than    1734 bp
  90% of sequence in fragments shorter than    2033 bp
  95% of sequence in fragments shorter than    2568 bp

# BthCI.SwaI (GCNGC+ATTTAAAT) double digest, 6436 thousand fragments (6209929+226602 sites), 463 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     444 bp
  25% of sequence in fragments shorter than     540 bp
  30% of sequence in fragments shorter than     636 bp
  35% of sequence in fragments shorter than     735 bp
  40% of sequence in fragments shorter than     838 bp
  45% of sequence in fragments shorter than     945 bp
  50% of sequence in fragments shorter than    1060 bp
  55% of sequence in fragments shorter than    1188 bp
  60% of sequence in fragments shorter than    1326 bp
  65% of sequence in fragments shorter than    1480 bp
  70% of sequence in fragments shorter than    1650 bp
  75% of sequence in fragments shorter than    1855 bp
  80% of sequence in fragments shorter than    2091 bp
  85% of sequence in fragments shorter than    2412 bp
  90% of sequence in fragments shorter than    2857 bp
  95% of sequence in fragments shorter than    3616 bp

# CviQI.PstI (GTAC+CTGCAG) double digest, 6436 thousand fragments (5115078+1321469 sites), 463 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     321 bp
  20% of sequence in fragments shorter than     390 bp
  25% of sequence in fragments shorter than     457 bp
  30% of sequence in fragments shorter than     521 bp
  35% of sequence in fragments shorter than     587 bp
  40% of sequence in fragments shorter than     657 bp
  45% of sequence in fragments shorter than     728 bp
  50% of sequence in fragments shorter than     804 bp
  55% of sequence in fragments shorter than     886 bp
  60% of sequence in fragments shorter than     973 bp
  65% of sequence in fragments shorter than    1071 bp
  70% of sequence in fragments shorter than    1184 bp
  75% of sequence in fragments shorter than    1307 bp
  80% of sequence in fragments shorter than    1448 bp
  85% of sequence in fragments shorter than    1642 bp
  90% of sequence in fragments shorter than    1918 bp
  95% of sequence in fragments shorter than    2376 bp

# AgeI.ApoI (ACCGGT+RAATTY) double digest, 6435 thousand fragments (53386+6382433 sites), 463 bp average fragment length
   5% of sequence in fragments shorter than     150 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     454 bp
  30% of sequence in fragments shorter than     531 bp
  35% of sequence in fragments shorter than     609 bp
  40% of sequence in fragments shorter than     690 bp
  45% of sequence in fragments shorter than     777 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     967 bp
  60% of sequence in fragments shorter than    1080 bp
  65% of sequence in fragments shorter than    1209 bp
  70% of sequence in fragments shorter than    1349 bp
  75% of sequence in fragments shorter than    1522 bp
  80% of sequence in fragments shorter than    1744 bp
  85% of sequence in fragments shorter than    2048 bp
  90% of sequence in fragments shorter than    2508 bp
  95% of sequence in fragments shorter than    3463 bp

# BscGI.FokI (CCCGT+GGATG) double digest, 6431 thousand fragments (1231070+5201116 sites), 463 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     460 bp
  30% of sequence in fragments shorter than     533 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     689 bp
  45% of sequence in fragments shorter than     772 bp
  50% of sequence in fragments shorter than     861 bp
  55% of sequence in fragments shorter than     952 bp
  60% of sequence in fragments shorter than    1057 bp
  65% of sequence in fragments shorter than    1170 bp
  70% of sequence in fragments shorter than    1300 bp
  75% of sequence in fragments shorter than    1444 bp
  80% of sequence in fragments shorter than    1625 bp
  85% of sequence in fragments shorter than    1858 bp
  90% of sequence in fragments shorter than    2182 bp
  95% of sequence in fragments shorter than    2737 bp

# ApoI.PmeI (RAATTY+GTTTAAAC) double digest, 6424 thousand fragments (6382433+42094 sites), 463 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     237 bp
  15% of sequence in fragments shorter than     309 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     455 bp
  30% of sequence in fragments shorter than     532 bp
  35% of sequence in fragments shorter than     610 bp
  40% of sequence in fragments shorter than     691 bp
  45% of sequence in fragments shorter than     779 bp
  50% of sequence in fragments shorter than     873 bp
  55% of sequence in fragments shorter than     972 bp
  60% of sequence in fragments shorter than    1086 bp
  65% of sequence in fragments shorter than    1215 bp
  70% of sequence in fragments shorter than    1358 bp
  75% of sequence in fragments shorter than    1533 bp
  80% of sequence in fragments shorter than    1761 bp
  85% of sequence in fragments shorter than    2073 bp
  90% of sequence in fragments shorter than    2552 bp
  95% of sequence in fragments shorter than    3556 bp

# FauI.TfiI (CCCGC+GAWTC) double digest, 6423 thousand fragments (1439772+4984362 sites), 463 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     243 bp
  15% of sequence in fragments shorter than     320 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     451 bp
  30% of sequence in fragments shorter than     522 bp
  35% of sequence in fragments shorter than     598 bp
  40% of sequence in fragments shorter than     673 bp
  45% of sequence in fragments shorter than     748 bp
  50% of sequence in fragments shorter than     828 bp
  55% of sequence in fragments shorter than     916 bp
  60% of sequence in fragments shorter than    1013 bp
  65% of sequence in fragments shorter than    1117 bp
  70% of sequence in fragments shorter than    1237 bp
  75% of sequence in fragments shorter than    1374 bp
  80% of sequence in fragments shorter than    1539 bp
  85% of sequence in fragments shorter than    1749 bp
  90% of sequence in fragments shorter than    2035 bp
  95% of sequence in fragments shorter than    2517 bp

# BspMI.TfiI (ACCTGC+GAWTC) double digest, 6421 thousand fragments (1438129+4984362 sites), 464 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     316 bp
  20% of sequence in fragments shorter than     372 bp
  25% of sequence in fragments shorter than     438 bp
  30% of sequence in fragments shorter than     507 bp
  35% of sequence in fragments shorter than     573 bp
  40% of sequence in fragments shorter than     646 bp
  45% of sequence in fragments shorter than     716 bp
  50% of sequence in fragments shorter than     793 bp
  55% of sequence in fragments shorter than     871 bp
  60% of sequence in fragments shorter than     962 bp
  65% of sequence in fragments shorter than    1062 bp
  70% of sequence in fragments shorter than    1175 bp
  75% of sequence in fragments shorter than    1303 bp
  80% of sequence in fragments shorter than    1459 bp
  85% of sequence in fragments shorter than    1656 bp
  90% of sequence in fragments shorter than    1924 bp
  95% of sequence in fragments shorter than    2374 bp

# ApoI.SalI (RAATTY+GTCGAC) double digest, 6413 thousand fragments (6382433+31396 sites), 464 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     310 bp
  20% of sequence in fragments shorter than     382 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     533 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     692 bp
  45% of sequence in fragments shorter than     780 bp
  50% of sequence in fragments shorter than     873 bp
  55% of sequence in fragments shorter than     972 bp
  60% of sequence in fragments shorter than    1085 bp
  65% of sequence in fragments shorter than    1215 bp
  70% of sequence in fragments shorter than    1356 bp
  75% of sequence in fragments shorter than    1530 bp
  80% of sequence in fragments shorter than    1756 bp
  85% of sequence in fragments shorter than    2064 bp
  90% of sequence in fragments shorter than    2534 bp
  95% of sequence in fragments shorter than    3514 bp

# HphI.SpeI (GGTGA+ACTAGT) double digest, 6407 thousand fragments (6007336+400286 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     313 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     448 bp
  30% of sequence in fragments shorter than     516 bp
  35% of sequence in fragments shorter than     584 bp
  40% of sequence in fragments shorter than     653 bp
  45% of sequence in fragments shorter than     726 bp
  50% of sequence in fragments shorter than     803 bp
  55% of sequence in fragments shorter than     887 bp
  60% of sequence in fragments shorter than     981 bp
  65% of sequence in fragments shorter than    1086 bp
  70% of sequence in fragments shorter than    1204 bp
  75% of sequence in fragments shorter than    1338 bp
  80% of sequence in fragments shorter than    1488 bp
  85% of sequence in fragments shorter than    1700 bp
  90% of sequence in fragments shorter than    1999 bp
  95% of sequence in fragments shorter than    2504 bp

# BccI.BsrBI (CCATC+CCGCTC) double digest, 6405 thousand fragments (6170927+235523 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     242 bp
  15% of sequence in fragments shorter than     316 bp
  20% of sequence in fragments shorter than     387 bp
  25% of sequence in fragments shorter than     455 bp
  30% of sequence in fragments shorter than     526 bp
  35% of sequence in fragments shorter than     600 bp
  40% of sequence in fragments shorter than     675 bp
  45% of sequence in fragments shorter than     756 bp
  50% of sequence in fragments shorter than     842 bp
  55% of sequence in fragments shorter than     934 bp
  60% of sequence in fragments shorter than    1037 bp
  65% of sequence in fragments shorter than    1147 bp
  70% of sequence in fragments shorter than    1276 bp
  75% of sequence in fragments shorter than    1429 bp
  80% of sequence in fragments shorter than    1617 bp
  85% of sequence in fragments shorter than    1860 bp
  90% of sequence in fragments shorter than    2213 bp
  95% of sequence in fragments shorter than    2863 bp

# ApoI.MluI (RAATTY+ACGCGT) double digest, 6403 thousand fragments (6382433+21415 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     310 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     457 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     612 bp
  40% of sequence in fragments shorter than     692 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     875 bp
  55% of sequence in fragments shorter than     973 bp
  60% of sequence in fragments shorter than    1087 bp
  65% of sequence in fragments shorter than    1216 bp
  70% of sequence in fragments shorter than    1358 bp
  75% of sequence in fragments shorter than    1532 bp
  80% of sequence in fragments shorter than    1758 bp
  85% of sequence in fragments shorter than    2065 bp
  90% of sequence in fragments shorter than    2534 bp
  95% of sequence in fragments shorter than    3507 bp

# BccI.KasI (CCATC+GGCGCC) double digest, 6400 thousand fragments (6170927+230381 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     243 bp
  15% of sequence in fragments shorter than     318 bp
  20% of sequence in fragments shorter than     390 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     531 bp
  35% of sequence in fragments shorter than     606 bp
  40% of sequence in fragments shorter than     682 bp
  45% of sequence in fragments shorter than     763 bp
  50% of sequence in fragments shorter than     850 bp
  55% of sequence in fragments shorter than     944 bp
  60% of sequence in fragments shorter than    1048 bp
  65% of sequence in fragments shorter than    1157 bp
  70% of sequence in fragments shorter than    1287 bp
  75% of sequence in fragments shorter than    1443 bp
  80% of sequence in fragments shorter than    1632 bp
  85% of sequence in fragments shorter than    1879 bp
  90% of sequence in fragments shorter than    2237 bp
  95% of sequence in fragments shorter than    2909 bp

# AvaII.BscAI (GGWCC+GCATC) double digest, 6400 thousand fragments (2747690+3652926 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     153 bp
  10% of sequence in fragments shorter than     236 bp
  15% of sequence in fragments shorter than     313 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     462 bp
  30% of sequence in fragments shorter than     538 bp
  35% of sequence in fragments shorter than     617 bp
  40% of sequence in fragments shorter than     697 bp
  45% of sequence in fragments shorter than     785 bp
  50% of sequence in fragments shorter than     877 bp
  55% of sequence in fragments shorter than     978 bp
  60% of sequence in fragments shorter than    1089 bp
  65% of sequence in fragments shorter than    1211 bp
  70% of sequence in fragments shorter than    1353 bp
  75% of sequence in fragments shorter than    1511 bp
  80% of sequence in fragments shorter than    1709 bp
  85% of sequence in fragments shorter than    1965 bp
  90% of sequence in fragments shorter than    2323 bp
  95% of sequence in fragments shorter than    2958 bp

# SspI.Tsp45I (AATATT+GTSAC) double digest, 6398 thousand fragments (2377267+4021761 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     245 bp
  15% of sequence in fragments shorter than     316 bp
  20% of sequence in fragments shorter than     379 bp
  25% of sequence in fragments shorter than     445 bp
  30% of sequence in fragments shorter than     512 bp
  35% of sequence in fragments shorter than     579 bp
  40% of sequence in fragments shorter than     649 bp
  45% of sequence in fragments shorter than     720 bp
  50% of sequence in fragments shorter than     798 bp
  55% of sequence in fragments shorter than     879 bp
  60% of sequence in fragments shorter than     971 bp
  65% of sequence in fragments shorter than    1073 bp
  70% of sequence in fragments shorter than    1190 bp
  75% of sequence in fragments shorter than    1327 bp
  80% of sequence in fragments shorter than    1491 bp
  85% of sequence in fragments shorter than    1710 bp
  90% of sequence in fragments shorter than    2021 bp
  95% of sequence in fragments shorter than    2611 bp

# ApoI.SgrAI (RAATTY+CRCCGGYG) double digest, 6397 thousand fragments (6382433+15270 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     457 bp
  30% of sequence in fragments shorter than     535 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     875 bp
  55% of sequence in fragments shorter than     974 bp
  60% of sequence in fragments shorter than    1088 bp
  65% of sequence in fragments shorter than    1217 bp
  70% of sequence in fragments shorter than    1359 bp
  75% of sequence in fragments shorter than    1532 bp
  80% of sequence in fragments shorter than    1757 bp
  85% of sequence in fragments shorter than    2064 bp
  90% of sequence in fragments shorter than    2531 bp
  95% of sequence in fragments shorter than    3498 bp

# BccI.SwaI (CCATC+ATTTAAAT) double digest, 6397 thousand fragments (6170927+226602 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     243 bp
  15% of sequence in fragments shorter than     317 bp
  20% of sequence in fragments shorter than     387 bp
  25% of sequence in fragments shorter than     454 bp
  30% of sequence in fragments shorter than     524 bp
  35% of sequence in fragments shorter than     598 bp
  40% of sequence in fragments shorter than     670 bp
  45% of sequence in fragments shorter than     749 bp
  50% of sequence in fragments shorter than     832 bp
  55% of sequence in fragments shorter than     921 bp
  60% of sequence in fragments shorter than    1020 bp
  65% of sequence in fragments shorter than    1124 bp
  70% of sequence in fragments shorter than    1247 bp
  75% of sequence in fragments shorter than    1392 bp
  80% of sequence in fragments shorter than    1570 bp
  85% of sequence in fragments shorter than    1798 bp
  90% of sequence in fragments shorter than    2133 bp
  95% of sequence in fragments shorter than    2749 bp

# ApoI.NruI (RAATTY+TCGCGA) double digest, 6396 thousand fragments (6382433+14872 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     457 bp
  30% of sequence in fragments shorter than     535 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     875 bp
  55% of sequence in fragments shorter than     974 bp
  60% of sequence in fragments shorter than    1088 bp
  65% of sequence in fragments shorter than    1218 bp
  70% of sequence in fragments shorter than    1360 bp
  75% of sequence in fragments shorter than    1535 bp
  80% of sequence in fragments shorter than    1762 bp
  85% of sequence in fragments shorter than    2072 bp
  90% of sequence in fragments shorter than    2546 bp
  95% of sequence in fragments shorter than    3532 bp

# HpaI.HphI (GTTAAC+GGTGA) double digest, 6396 thousand fragments (389418+6007336 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     314 bp
  20% of sequence in fragments shorter than     380 bp
  25% of sequence in fragments shorter than     449 bp
  30% of sequence in fragments shorter than     516 bp
  35% of sequence in fragments shorter than     584 bp
  40% of sequence in fragments shorter than     653 bp
  45% of sequence in fragments shorter than     726 bp
  50% of sequence in fragments shorter than     803 bp
  55% of sequence in fragments shorter than     887 bp
  60% of sequence in fragments shorter than     982 bp
  65% of sequence in fragments shorter than    1087 bp
  70% of sequence in fragments shorter than    1208 bp
  75% of sequence in fragments shorter than    1344 bp
  80% of sequence in fragments shorter than    1503 bp
  85% of sequence in fragments shorter than    1721 bp
  90% of sequence in fragments shorter than    2029 bp
  95% of sequence in fragments shorter than    2552 bp

# ApoI.PvuI (RAATTY+CGATCG) double digest, 6396 thousand fragments (6382433+14186 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     457 bp
  30% of sequence in fragments shorter than     535 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     876 bp
  55% of sequence in fragments shorter than     975 bp
  60% of sequence in fragments shorter than    1089 bp
  65% of sequence in fragments shorter than    1219 bp
  70% of sequence in fragments shorter than    1362 bp
  75% of sequence in fragments shorter than    1537 bp
  80% of sequence in fragments shorter than    1764 bp
  85% of sequence in fragments shorter than    2075 bp
  90% of sequence in fragments shorter than    2550 bp
  95% of sequence in fragments shorter than    3543 bp

# ApoI.FseI (RAATTY+GGCCGGCC) double digest, 6395 thousand fragments (6382433+13662 sites), 465 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     383 bp
  25% of sequence in fragments shorter than     457 bp
  30% of sequence in fragments shorter than     535 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     876 bp
  55% of sequence in fragments shorter than     975 bp
  60% of sequence in fragments shorter than    1088 bp
  65% of sequence in fragments shorter than    1218 bp
  70% of sequence in fragments shorter than    1359 bp
  75% of sequence in fragments shorter than    1533 bp
  80% of sequence in fragments shorter than    1759 bp
  85% of sequence in fragments shorter than    2065 bp
  90% of sequence in fragments shorter than    2532 bp
  95% of sequence in fragments shorter than    3494 bp

# ApoI.NotI (RAATTY+GCGGCCGC) double digest, 6391 thousand fragments (6382433+9795 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     535 bp
  35% of sequence in fragments shorter than     614 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     783 bp
  50% of sequence in fragments shorter than     876 bp
  55% of sequence in fragments shorter than     975 bp
  60% of sequence in fragments shorter than    1089 bp
  65% of sequence in fragments shorter than    1219 bp
  70% of sequence in fragments shorter than    1361 bp
  75% of sequence in fragments shorter than    1535 bp
  80% of sequence in fragments shorter than    1761 bp
  85% of sequence in fragments shorter than    2069 bp
  90% of sequence in fragments shorter than    2540 bp
  95% of sequence in fragments shorter than    3517 bp

# ApoI.RsrII (RAATTY+CGGWCCG) double digest, 6391 thousand fragments (6382433+9764 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     535 bp
  35% of sequence in fragments shorter than     614 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     783 bp
  50% of sequence in fragments shorter than     876 bp
  55% of sequence in fragments shorter than     976 bp
  60% of sequence in fragments shorter than    1089 bp
  65% of sequence in fragments shorter than    1219 bp
  70% of sequence in fragments shorter than    1361 bp
  75% of sequence in fragments shorter than    1536 bp
  80% of sequence in fragments shorter than    1763 bp
  85% of sequence in fragments shorter than    2072 bp
  90% of sequence in fragments shorter than    2544 bp
  95% of sequence in fragments shorter than    3525 bp

# AccI.BbvI (GTMKAC+GCAGC) double digest, 6390 thousand fragments (1101063+5290051 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     332 bp
  20% of sequence in fragments shorter than     414 bp
  25% of sequence in fragments shorter than     496 bp
  30% of sequence in fragments shorter than     581 bp
  35% of sequence in fragments shorter than     667 bp
  40% of sequence in fragments shorter than     757 bp
  45% of sequence in fragments shorter than     850 bp
  50% of sequence in fragments shorter than     947 bp
  55% of sequence in fragments shorter than    1055 bp
  60% of sequence in fragments shorter than    1176 bp
  65% of sequence in fragments shorter than    1306 bp
  70% of sequence in fragments shorter than    1456 bp
  75% of sequence in fragments shorter than    1625 bp
  80% of sequence in fragments shorter than    1827 bp
  85% of sequence in fragments shorter than    2081 bp
  90% of sequence in fragments shorter than    2462 bp
  95% of sequence in fragments shorter than    3097 bp

# ApoI.AscI (RAATTY+GGCGCGCC) double digest, 6386 thousand fragments (6382433+4751 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     536 bp
  35% of sequence in fragments shorter than     614 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     784 bp
  50% of sequence in fragments shorter than     877 bp
  55% of sequence in fragments shorter than     977 bp
  60% of sequence in fragments shorter than    1091 bp
  65% of sequence in fragments shorter than    1221 bp
  70% of sequence in fragments shorter than    1364 bp
  75% of sequence in fragments shorter than    1539 bp
  80% of sequence in fragments shorter than    1767 bp
  85% of sequence in fragments shorter than    2078 bp
  90% of sequence in fragments shorter than    2555 bp
  95% of sequence in fragments shorter than    3548 bp

# AflII.BsrI (CTTAAG+ACTGG) double digest, 6384 thousand fragments (643284+5741310 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     240 bp
  15% of sequence in fragments shorter than     315 bp
  20% of sequence in fragments shorter than     385 bp
  25% of sequence in fragments shorter than     454 bp
  30% of sequence in fragments shorter than     519 bp
  35% of sequence in fragments shorter than     589 bp
  40% of sequence in fragments shorter than     661 bp
  45% of sequence in fragments shorter than     730 bp
  50% of sequence in fragments shorter than     811 bp
  55% of sequence in fragments shorter than     894 bp
  60% of sequence in fragments shorter than     989 bp
  65% of sequence in fragments shorter than    1093 bp
  70% of sequence in fragments shorter than    1211 bp
  75% of sequence in fragments shorter than    1350 bp
  80% of sequence in fragments shorter than    1512 bp
  85% of sequence in fragments shorter than    1725 bp
  90% of sequence in fragments shorter than    2022 bp
  95% of sequence in fragments shorter than    2570 bp

# HphI.SapI (GGTGA+GCTCTTC) double digest, 6384 thousand fragments (6007336+377161 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     315 bp
  20% of sequence in fragments shorter than     381 bp
  25% of sequence in fragments shorter than     451 bp
  30% of sequence in fragments shorter than     519 bp
  35% of sequence in fragments shorter than     586 bp
  40% of sequence in fragments shorter than     659 bp
  45% of sequence in fragments shorter than     733 bp
  50% of sequence in fragments shorter than     812 bp
  55% of sequence in fragments shorter than     896 bp
  60% of sequence in fragments shorter than     994 bp
  65% of sequence in fragments shorter than    1101 bp
  70% of sequence in fragments shorter than    1225 bp
  75% of sequence in fragments shorter than    1367 bp
  80% of sequence in fragments shorter than    1535 bp
  85% of sequence in fragments shorter than    1761 bp
  90% of sequence in fragments shorter than    2071 bp
  95% of sequence in fragments shorter than    2625 bp

# HphI.SmaI (GGTGA+CCCGGG) double digest, 6383 thousand fragments (6007336+376940 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     315 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     527 bp
  35% of sequence in fragments shorter than     598 bp
  40% of sequence in fragments shorter than     674 bp
  45% of sequence in fragments shorter than     752 bp
  50% of sequence in fragments shorter than     835 bp
  55% of sequence in fragments shorter than     926 bp
  60% of sequence in fragments shorter than    1029 bp
  65% of sequence in fragments shorter than    1142 bp
  70% of sequence in fragments shorter than    1271 bp
  75% of sequence in fragments shorter than    1409 bp
  80% of sequence in fragments shorter than    1590 bp
  85% of sequence in fragments shorter than    1831 bp
  90% of sequence in fragments shorter than    2160 bp
  95% of sequence in fragments shorter than    2744 bp

# AclI.BthCI (AACGTT+GCNGC) double digest, 6382 thousand fragments (172706+6209929 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     351 bp
  20% of sequence in fragments shorter than     447 bp
  25% of sequence in fragments shorter than     545 bp
  30% of sequence in fragments shorter than     645 bp
  35% of sequence in fragments shorter than     748 bp
  40% of sequence in fragments shorter than     856 bp
  45% of sequence in fragments shorter than     966 bp
  50% of sequence in fragments shorter than    1086 bp
  55% of sequence in fragments shorter than    1220 bp
  60% of sequence in fragments shorter than    1364 bp
  65% of sequence in fragments shorter than    1526 bp
  70% of sequence in fragments shorter than    1705 bp
  75% of sequence in fragments shorter than    1920 bp
  80% of sequence in fragments shorter than    2170 bp
  85% of sequence in fragments shorter than    2507 bp
  90% of sequence in fragments shorter than    2973 bp
  95% of sequence in fragments shorter than    3777 bp

# ApoI.EcoRI (RAATTY+GAATTC) double digest, 6381 thousand fragments (6382433+847401 sites), 466 bp average fragment length
   5% of sequence in fragments shorter than     151 bp
  10% of sequence in fragments shorter than     238 bp
  15% of sequence in fragments shorter than     311 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     536 bp
  35% of sequence in fragments shorter than     615 bp
  40% of sequence in fragments shorter than     695 bp
  45% of sequence in fragments shorter than     785 bp
  50% of sequence in fragments shorter than     878 bp
  55% of sequence in fragments shorter than     978 bp
  60% of sequence in fragments shorter than    1093 bp
  65% of sequence in fragments shorter than    1223 bp
  70% of sequence in fragments shorter than    1367 bp
  75% of sequence in fragments shorter than    1543 bp
  80% of sequence in fragments shorter than    1773 bp
  85% of sequence in fragments shorter than    2086 bp
  90% of sequence in fragments shorter than    2571 bp
  95% of sequence in fragments shorter than    3582 bp

# BscAI.SmlI (GCATC+CTYRAG) double digest, 6380 thousand fragments (3652926+2727867 sites), 467 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     242 bp
  15% of sequence in fragments shorter than     315 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     451 bp
  30% of sequence in fragments shorter than     518 bp
  35% of sequence in fragments shorter than     588 bp
  40% of sequence in fragments shorter than     659 bp
  45% of sequence in fragments shorter than     733 bp
  50% of sequence in fragments shorter than     812 bp
  55% of sequence in fragments shorter than     897 bp
  60% of sequence in fragments shorter than     990 bp
  65% of sequence in fragments shorter than    1094 bp
  70% of sequence in fragments shorter than    1211 bp
  75% of sequence in fragments shorter than    1345 bp
  80% of sequence in fragments shorter than    1507 bp
  85% of sequence in fragments shorter than    1707 bp
  90% of sequence in fragments shorter than    1999 bp
  95% of sequence in fragments shorter than    2513 bp

# BbvI.PpuMI (GCAGC+RGGWCCY) double digest, 6374 thousand fragments (5290051+1085138 sites), 467 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     332 bp
  20% of sequence in fragments shorter than     422 bp
  25% of sequence in fragments shorter than     515 bp
  30% of sequence in fragments shorter than     610 bp
  35% of sequence in fragments shorter than     703 bp
  40% of sequence in fragments shorter than     807 bp
  45% of sequence in fragments shorter than     911 bp
  50% of sequence in fragments shorter than    1023 bp
  55% of sequence in fragments shorter than    1150 bp
  60% of sequence in fragments shorter than    1285 bp
  65% of sequence in fragments shorter than    1441 bp
  70% of sequence in fragments shorter than    1622 bp
  75% of sequence in fragments shorter than    1830 bp
  80% of sequence in fragments shorter than    2076 bp
  85% of sequence in fragments shorter than    2407 bp
  90% of sequence in fragments shorter than    2867 bp
  95% of sequence in fragments shorter than    3679 bp

# BbvI.PvuII (GCAGC+CAGCTG) double digest, 6373 thousand fragments (5290051+1084260 sites), 467 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     456 bp
  25% of sequence in fragments shorter than     554 bp
  30% of sequence in fragments shorter than     653 bp
  35% of sequence in fragments shorter than     756 bp
  40% of sequence in fragments shorter than     861 bp
  45% of sequence in fragments shorter than     972 bp
  50% of sequence in fragments shorter than    1094 bp
  55% of sequence in fragments shorter than    1226 bp
  60% of sequence in fragments shorter than    1372 bp
  65% of sequence in fragments shorter than    1535 bp
  70% of sequence in fragments shorter than    1710 bp
  75% of sequence in fragments shorter than    1922 bp
  80% of sequence in fragments shorter than    2170 bp
  85% of sequence in fragments shorter than    2511 bp
  90% of sequence in fragments shorter than    2984 bp
  95% of sequence in fragments shorter than    3817 bp

# BamHI.HphI (GGATCC+GGTGA) double digest, 6372 thousand fragments (365999+6007336 sites), 467 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     315 bp
  20% of sequence in fragments shorter than     382 bp
  25% of sequence in fragments shorter than     451 bp
  30% of sequence in fragments shorter than     520 bp
  35% of sequence in fragments shorter than     589 bp
  40% of sequence in fragments shorter than     661 bp
  45% of sequence in fragments shorter than     736 bp
  50% of sequence in fragments shorter than     815 bp
  55% of sequence in fragments shorter than     899 bp
  60% of sequence in fragments shorter than     998 bp
  65% of sequence in fragments shorter than    1106 bp
  70% of sequence in fragments shorter than    1231 bp
  75% of sequence in fragments shorter than    1373 bp
  80% of sequence in fragments shorter than    1536 bp
  85% of sequence in fragments shorter than    1760 bp
  90% of sequence in fragments shorter than    2069 bp
  95% of sequence in fragments shorter than    2624 bp

# BsrI.HaeII (ACTGG+RGCGCY) double digest, 6370 thousand fragments (5741310+630101 sites), 467 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     317 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     455 bp
  30% of sequence in fragments shorter than     524 bp
  35% of sequence in fragments shorter than     597 bp
  40% of sequence in fragments shorter than     670 bp
  45% of sequence in fragments shorter than     735 bp
  50% of sequence in fragments shorter than     818 bp
  55% of sequence in fragments shorter than     902 bp
  60% of sequence in fragments shorter than     999 bp
  65% of sequence in fragments shorter than    1105 bp
  70% of sequence in fragments shorter than    1225 bp
  75% of sequence in fragments shorter than    1367 bp
  80% of sequence in fragments shorter than    1535 bp
  85% of sequence in fragments shorter than    1746 bp
  90% of sequence in fragments shorter than    2050 bp
  95% of sequence in fragments shorter than    2559 bp

# BthCI.PacI (GCNGC+TTAATTAA) double digest, 6368 thousand fragments (6209929+159455 sites), 467 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     260 bp
  15% of sequence in fragments shorter than     353 bp
  20% of sequence in fragments shorter than     450 bp
  25% of sequence in fragments shorter than     548 bp
  30% of sequence in fragments shorter than     647 bp
  35% of sequence in fragments shorter than     750 bp
  40% of sequence in fragments shorter than     856 bp
  45% of sequence in fragments shorter than     966 bp
  50% of sequence in fragments shorter than    1084 bp
  55% of sequence in fragments shorter than    1216 bp
  60% of sequence in fragments shorter than    1361 bp
  65% of sequence in fragments shorter than    1522 bp
  70% of sequence in fragments shorter than    1698 bp
  75% of sequence in fragments shorter than    1911 bp
  80% of sequence in fragments shorter than    2158 bp
  85% of sequence in fragments shorter than    2493 bp
  90% of sequence in fragments shorter than    2949 bp
  95% of sequence in fragments shorter than    3752 bp

# BthCI.Hpy99I (GCNGC+CGWCG) double digest, 6367 thousand fragments (6209929+157917 sites), 468 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     260 bp
  15% of sequence in fragments shorter than     355 bp
  20% of sequence in fragments shorter than     455 bp
  25% of sequence in fragments shorter than     556 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     767 bp
  40% of sequence in fragments shorter than     873 bp
  45% of sequence in fragments shorter than     992 bp
  50% of sequence in fragments shorter than    1115 bp
  55% of sequence in fragments shorter than    1252 bp
  60% of sequence in fragments shorter than    1402 bp
  65% of sequence in fragments shorter than    1574 bp
  70% of sequence in fragments shorter than    1760 bp
  75% of sequence in fragments shorter than    1984 bp
  80% of sequence in fragments shorter than    2248 bp
  85% of sequence in fragments shorter than    2603 bp
  90% of sequence in fragments shorter than    3087 bp
  95% of sequence in fragments shorter than    3938 bp

# BscGI.CviQI (CCCGT+GTAC) double digest, 6345 thousand fragments (1231070+5115078 sites), 469 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     326 bp
  20% of sequence in fragments shorter than     387 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     521 bp
  35% of sequence in fragments shorter than     589 bp
  40% of sequence in fragments shorter than     661 bp
  45% of sequence in fragments shorter than     732 bp
  50% of sequence in fragments shorter than     809 bp
  55% of sequence in fragments shorter than     892 bp
  60% of sequence in fragments shorter than     982 bp
  65% of sequence in fragments shorter than    1082 bp
  70% of sequence in fragments shorter than    1194 bp
  75% of sequence in fragments shorter than    1317 bp
  80% of sequence in fragments shorter than    1459 bp
  85% of sequence in fragments shorter than    1650 bp
  90% of sequence in fragments shorter than    1917 bp
  95% of sequence in fragments shorter than    2362 bp

# BsrI.SacI (ACTGG+GAGCTC) double digest, 6344 thousand fragments (5741310+603323 sites), 469 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     317 bp
  20% of sequence in fragments shorter than     388 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     528 bp
  35% of sequence in fragments shorter than     601 bp
  40% of sequence in fragments shorter than     674 bp
  45% of sequence in fragments shorter than     744 bp
  50% of sequence in fragments shorter than     828 bp
  55% of sequence in fragments shorter than     915 bp
  60% of sequence in fragments shorter than    1013 bp
  65% of sequence in fragments shorter than    1119 bp
  70% of sequence in fragments shorter than    1242 bp
  75% of sequence in fragments shorter than    1385 bp
  80% of sequence in fragments shorter than    1558 bp
  85% of sequence in fragments shorter than    1779 bp
  90% of sequence in fragments shorter than    2090 bp
  95% of sequence in fragments shorter than    2658 bp

# AclI.BccI (AACGTT+CCATC) double digest, 6343 thousand fragments (172706+6170927 sites), 469 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     245 bp
  15% of sequence in fragments shorter than     320 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     531 bp
  35% of sequence in fragments shorter than     606 bp
  40% of sequence in fragments shorter than     681 bp
  45% of sequence in fragments shorter than     760 bp
  50% of sequence in fragments shorter than     845 bp
  55% of sequence in fragments shorter than     937 bp
  60% of sequence in fragments shorter than    1036 bp
  65% of sequence in fragments shorter than    1143 bp
  70% of sequence in fragments shorter than    1269 bp
  75% of sequence in fragments shorter than    1418 bp
  80% of sequence in fragments shorter than    1600 bp
  85% of sequence in fragments shorter than    1834 bp
  90% of sequence in fragments shorter than    2167 bp
  95% of sequence in fragments shorter than    2773 bp

# BstEII.HphI (GGTNACC+GGTGA) double digest, 6342 thousand fragments (335865+6007336 sites), 469 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     327 bp
  20% of sequence in fragments shorter than     397 bp
  25% of sequence in fragments shorter than     469 bp
  30% of sequence in fragments shorter than     540 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     686 bp
  45% of sequence in fragments shorter than     764 bp
  50% of sequence in fragments shorter than     847 bp
  55% of sequence in fragments shorter than     938 bp
  60% of sequence in fragments shorter than    1039 bp
  65% of sequence in fragments shorter than    1152 bp
  70% of sequence in fragments shorter than    1280 bp
  75% of sequence in fragments shorter than    1415 bp
  80% of sequence in fragments shorter than    1594 bp
  85% of sequence in fragments shorter than    1831 bp
  90% of sequence in fragments shorter than    2153 bp
  95% of sequence in fragments shorter than    2728 bp

# BthCI.SnaBI (GCNGC+TACGTA) double digest, 6341 thousand fragments (6209929+132299 sites), 469 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     261 bp
  15% of sequence in fragments shorter than     354 bp
  20% of sequence in fragments shorter than     452 bp
  25% of sequence in fragments shorter than     552 bp
  30% of sequence in fragments shorter than     652 bp
  35% of sequence in fragments shorter than     756 bp
  40% of sequence in fragments shorter than     862 bp
  45% of sequence in fragments shorter than     975 bp
  50% of sequence in fragments shorter than    1094 bp
  55% of sequence in fragments shorter than    1228 bp
  60% of sequence in fragments shorter than    1376 bp
  65% of sequence in fragments shorter than    1539 bp
  70% of sequence in fragments shorter than    1720 bp
  75% of sequence in fragments shorter than    1936 bp
  80% of sequence in fragments shorter than    2189 bp
  85% of sequence in fragments shorter than    2531 bp
  90% of sequence in fragments shorter than    2994 bp
  95% of sequence in fragments shorter than    3808 bp

# HpaII.Tsp45I (CCGG+GTSAC) double digest, 6338 thousand fragments (2317722+4021761 sites), 470 bp average fragment length
   5% of sequence in fragments shorter than     149 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     323 bp
  20% of sequence in fragments shorter than     399 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     558 bp
  35% of sequence in fragments shorter than     642 bp
  40% of sequence in fragments shorter than     728 bp
  45% of sequence in fragments shorter than     822 bp
  50% of sequence in fragments shorter than     921 bp
  55% of sequence in fragments shorter than    1030 bp
  60% of sequence in fragments shorter than    1151 bp
  65% of sequence in fragments shorter than    1284 bp
  70% of sequence in fragments shorter than    1441 bp
  75% of sequence in fragments shorter than    1630 bp
  80% of sequence in fragments shorter than    1861 bp
  85% of sequence in fragments shorter than    2163 bp
  90% of sequence in fragments shorter than    2611 bp
  95% of sequence in fragments shorter than    3443 bp

# AvrII.BsrI (CCTAGG+ACTGG) double digest, 6335 thousand fragments (594956+5741310 sites), 470 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     242 bp
  15% of sequence in fragments shorter than     317 bp
  20% of sequence in fragments shorter than     382 bp
  25% of sequence in fragments shorter than     453 bp
  30% of sequence in fragments shorter than     524 bp
  35% of sequence in fragments shorter than     597 bp
  40% of sequence in fragments shorter than     670 bp
  45% of sequence in fragments shorter than     743 bp
  50% of sequence in fragments shorter than     826 bp
  55% of sequence in fragments shorter than     913 bp
  60% of sequence in fragments shorter than    1011 bp
  65% of sequence in fragments shorter than    1118 bp
  70% of sequence in fragments shorter than    1241 bp
  75% of sequence in fragments shorter than    1384 bp
  80% of sequence in fragments shorter than    1555 bp
  85% of sequence in fragments shorter than    1776 bp
  90% of sequence in fragments shorter than    2089 bp
  95% of sequence in fragments shorter than    2661 bp

# BthCI.NaeI (GCNGC+GCCGGC) double digest, 6335 thousand fragments (6209929+125692 sites), 470 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     358 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     561 bp
  30% of sequence in fragments shorter than     665 bp
  35% of sequence in fragments shorter than     774 bp
  40% of sequence in fragments shorter than     880 bp
  45% of sequence in fragments shorter than    1000 bp
  50% of sequence in fragments shorter than    1124 bp
  55% of sequence in fragments shorter than    1262 bp
  60% of sequence in fragments shorter than    1413 bp
  65% of sequence in fragments shorter than    1587 bp
  70% of sequence in fragments shorter than    1772 bp
  75% of sequence in fragments shorter than    2000 bp
  80% of sequence in fragments shorter than    2265 bp
  85% of sequence in fragments shorter than    2624 bp
  90% of sequence in fragments shorter than    3110 bp
  95% of sequence in fragments shorter than    3968 bp

# BthCI.XhoI (GCNGC+CTCGAG) double digest, 6330 thousand fragments (6209929+121308 sites), 470 bp average fragment length
   5% of sequence in fragments shorter than     155 bp
  10% of sequence in fragments shorter than     261 bp
  15% of sequence in fragments shorter than     355 bp
  20% of sequence in fragments shorter than     454 bp
  25% of sequence in fragments shorter than     554 bp
  30% of sequence in fragments shorter than     657 bp
  35% of sequence in fragments shorter than     763 bp
  40% of sequence in fragments shorter than     870 bp
  45% of sequence in fragments shorter than     988 bp
  50% of sequence in fragments shorter than    1111 bp
  55% of sequence in fragments shorter than    1248 bp
  60% of sequence in fragments shorter than    1396 bp
  65% of sequence in fragments shorter than    1568 bp
  70% of sequence in fragments shorter than    1753 bp
  75% of sequence in fragments shorter than    1976 bp
  80% of sequence in fragments shorter than    2238 bp
  85% of sequence in fragments shorter than    2590 bp
  90% of sequence in fragments shorter than    3072 bp
  95% of sequence in fragments shorter than    3914 bp

# BccI.PacI (CCATC+TTAATTAA) double digest, 6329 thousand fragments (6170927+159455 sites), 470 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     246 bp
  15% of sequence in fragments shorter than     320 bp
  20% of sequence in fragments shorter than     392 bp
  25% of sequence in fragments shorter than     460 bp
  30% of sequence in fragments shorter than     532 bp
  35% of sequence in fragments shorter than     607 bp
  40% of sequence in fragments shorter than     681 bp
  45% of sequence in fragments shorter than     761 bp
  50% of sequence in fragments shorter than     845 bp
  55% of sequence in fragments shorter than     937 bp
  60% of sequence in fragments shorter than    1039 bp
  65% of sequence in fragments shorter than    1145 bp
  70% of sequence in fragments shorter than    1271 bp
  75% of sequence in fragments shorter than    1421 bp
  80% of sequence in fragments shorter than    1604 bp
  85% of sequence in fragments shorter than    1841 bp
  90% of sequence in fragments shorter than    2186 bp
  95% of sequence in fragments shorter than    2823 bp

# BccI.Hpy99I (CCATC+CGWCG) double digest, 6328 thousand fragments (6170927+157917 sites), 470 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     245 bp
  15% of sequence in fragments shorter than     320 bp
  20% of sequence in fragments shorter than     392 bp
  25% of sequence in fragments shorter than     461 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     609 bp
  40% of sequence in fragments shorter than     685 bp
  45% of sequence in fragments shorter than     766 bp
  50% of sequence in fragments shorter than     853 bp
  55% of sequence in fragments shorter than     947 bp
  60% of sequence in fragments shorter than    1051 bp
  65% of sequence in fragments shorter than    1160 bp
  70% of sequence in fragments shorter than    1289 bp
  75% of sequence in fragments shorter than    1445 bp
  80% of sequence in fragments shorter than    1633 bp
  85% of sequence in fragments shorter than    1880 bp
  90% of sequence in fragments shorter than    2237 bp
  95% of sequence in fragments shorter than    2903 bp

# BsrI.HgaI (ACTGG+GACGC) double digest, 6312 thousand fragments (5741310+571889 sites), 472 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     242 bp
  15% of sequence in fragments shorter than     318 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     461 bp
  30% of sequence in fragments shorter than     532 bp
  35% of sequence in fragments shorter than     606 bp
  40% of sequence in fragments shorter than     681 bp
  45% of sequence in fragments shorter than     752 bp
  50% of sequence in fragments shorter than     836 bp
  55% of sequence in fragments shorter than     925 bp
  60% of sequence in fragments shorter than    1026 bp
  65% of sequence in fragments shorter than    1134 bp
  70% of sequence in fragments shorter than    1259 bp
  75% of sequence in fragments shorter than    1403 bp
  80% of sequence in fragments shorter than    1579 bp
  85% of sequence in fragments shorter than    1803 bp
  90% of sequence in fragments shorter than    2120 bp
  95% of sequence in fragments shorter than    2689 bp

# BsrI.EciI (ACTGG+GGCGGA) double digest, 6312 thousand fragments (5741310+571273 sites), 472 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     316 bp
  20% of sequence in fragments shorter than     386 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     523 bp
  35% of sequence in fragments shorter than     595 bp
  40% of sequence in fragments shorter than     668 bp
  45% of sequence in fragments shorter than     736 bp
  50% of sequence in fragments shorter than     818 bp
  55% of sequence in fragments shorter than     901 bp
  60% of sequence in fragments shorter than     998 bp
  65% of sequence in fragments shorter than    1103 bp
  70% of sequence in fragments shorter than    1223 bp
  75% of sequence in fragments shorter than    1366 bp
  80% of sequence in fragments shorter than    1534 bp
  85% of sequence in fragments shorter than    1751 bp
  90% of sequence in fragments shorter than    2062 bp
  95% of sequence in fragments shorter than    2632 bp

# MlyI.SspI (GAGTC+AATATT) double digest, 6308 thousand fragments (3931692+2377267 sites), 472 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     318 bp
  20% of sequence in fragments shorter than     384 bp
  25% of sequence in fragments shorter than     449 bp
  30% of sequence in fragments shorter than     513 bp
  35% of sequence in fragments shorter than     580 bp
  40% of sequence in fragments shorter than     648 bp
  45% of sequence in fragments shorter than     719 bp
  50% of sequence in fragments shorter than     795 bp
  55% of sequence in fragments shorter than     875 bp
  60% of sequence in fragments shorter than     965 bp
  65% of sequence in fragments shorter than    1062 bp
  70% of sequence in fragments shorter than    1174 bp
  75% of sequence in fragments shorter than    1308 bp
  80% of sequence in fragments shorter than    1464 bp
  85% of sequence in fragments shorter than    1672 bp
  90% of sequence in fragments shorter than    1974 bp
  95% of sequence in fragments shorter than    2563 bp

# PstI.TfiI (CTGCAG+GAWTC) double digest, 6305 thousand fragments (1321469+4984362 sites), 472 bp average fragment length
   5% of sequence in fragments shorter than     169 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     376 bp
  25% of sequence in fragments shorter than     446 bp
  30% of sequence in fragments shorter than     515 bp
  35% of sequence in fragments shorter than     586 bp
  40% of sequence in fragments shorter than     658 bp
  45% of sequence in fragments shorter than     726 bp
  50% of sequence in fragments shorter than     801 bp
  55% of sequence in fragments shorter than     883 bp
  60% of sequence in fragments shorter than     973 bp
  65% of sequence in fragments shorter than    1072 bp
  70% of sequence in fragments shorter than    1186 bp
  75% of sequence in fragments shorter than    1316 bp
  80% of sequence in fragments shorter than    1472 bp
  85% of sequence in fragments shorter than    1670 bp
  90% of sequence in fragments shorter than    1941 bp
  95% of sequence in fragments shorter than    2398 bp

# BsrI.MfeI (ACTGG+CAATTG) double digest, 6305 thousand fragments (5741310+564303 sites), 472 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     244 bp
  15% of sequence in fragments shorter than     319 bp
  20% of sequence in fragments shorter than     389 bp
  25% of sequence in fragments shorter than     456 bp
  30% of sequence in fragments shorter than     526 bp
  35% of sequence in fragments shorter than     597 bp
  40% of sequence in fragments shorter than     669 bp
  45% of sequence in fragments shorter than     740 bp
  50% of sequence in fragments shorter than     822 bp
  55% of sequence in fragments shorter than     906 bp
  60% of sequence in fragments shorter than    1001 bp
  65% of sequence in fragments shorter than    1105 bp
  70% of sequence in fragments shorter than    1224 bp
  75% of sequence in fragments shorter than    1364 bp
  80% of sequence in fragments shorter than    1528 bp
  85% of sequence in fragments shorter than    1740 bp
  90% of sequence in fragments shorter than    2035 bp
  95% of sequence in fragments shorter than    2566 bp

# HphI.SexAI (GGTGA+ACCWGGT) double digest, 6304 thousand fragments (6007336+297900 sites), 472 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     245 bp
  15% of sequence in fragments shorter than     321 bp
  20% of sequence in fragments shorter than     390 bp
  25% of sequence in fragments shorter than     461 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     600 bp
  40% of sequence in fragments shorter than     674 bp
  45% of sequence in fragments shorter than     752 bp
  50% of sequence in fragments shorter than     833 bp
  55% of sequence in fragments shorter than     922 bp
  60% of sequence in fragments shorter than    1023 bp
  65% of sequence in fragments shorter than    1134 bp
  70% of sequence in fragments shorter than    1259 bp
  75% of sequence in fragments shorter than    1393 bp
  80% of sequence in fragments shorter than    1571 bp
  85% of sequence in fragments shorter than    1805 bp
  90% of sequence in fragments shorter than    2125 bp
  95% of sequence in fragments shorter than    2694 bp

# BccI.SnaBI (CCATC+TACGTA) double digest, 6302 thousand fragments (6170927+132299 sites), 472 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     393 bp
  25% of sequence in fragments shorter than     462 bp
  30% of sequence in fragments shorter than     534 bp
  35% of sequence in fragments shorter than     610 bp
  40% of sequence in fragments shorter than     685 bp
  45% of sequence in fragments shorter than     765 bp
  50% of sequence in fragments shorter than     850 bp
  55% of sequence in fragments shorter than     942 bp
  60% of sequence in fragments shorter than    1045 bp
  65% of sequence in fragments shorter than    1152 bp
  70% of sequence in fragments shorter than    1279 bp
  75% of sequence in fragments shorter than    1427 bp
  80% of sequence in fragments shorter than    1612 bp
  85% of sequence in fragments shorter than    1851 bp
  90% of sequence in fragments shorter than    2199 bp
  95% of sequence in fragments shorter than    2826 bp

# AccI.FokI (GTMKAC+GGATG) double digest, 6301 thousand fragments (1101063+5201116 sites), 472 bp average fragment length
   5% of sequence in fragments shorter than     164 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     324 bp
  20% of sequence in fragments shorter than     396 bp
  25% of sequence in fragments shorter than     468 bp
  30% of sequence in fragments shorter than     540 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     689 bp
  45% of sequence in fragments shorter than     768 bp
  50% of sequence in fragments shorter than     852 bp
  55% of sequence in fragments shorter than     938 bp
  60% of sequence in fragments shorter than    1036 bp
  65% of sequence in fragments shorter than    1140 bp
  70% of sequence in fragments shorter than    1257 bp
  75% of sequence in fragments shorter than    1391 bp
  80% of sequence in fragments shorter than    1556 bp
  85% of sequence in fragments shorter than    1766 bp
  90% of sequence in fragments shorter than    2065 bp
  95% of sequence in fragments shorter than    2594 bp

# BccI.NaeI (CCATC+GCCGGC) double digest, 6296 thousand fragments (6170927+125692 sites), 473 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     246 bp
  15% of sequence in fragments shorter than     321 bp
  20% of sequence in fragments shorter than     394 bp
  25% of sequence in fragments shorter than     463 bp
  30% of sequence in fragments shorter than     536 bp
  35% of sequence in fragments shorter than     612 bp
  40% of sequence in fragments shorter than     688 bp
  45% of sequence in fragments shorter than     770 bp
  50% of sequence in fragments shorter than     857 bp
  55% of sequence in fragments shorter than     951 bp
  60% of sequence in fragments shorter than    1056 bp
  65% of sequence in fragments shorter than    1165 bp
  70% of sequence in fragments shorter than    1295 bp
  75% of sequence in fragments shorter than    1452 bp
  80% of sequence in fragments shorter than    1642 bp
  85% of sequence in fragments shorter than    1889 bp
  90% of sequence in fragments shorter than    2249 bp
  95% of sequence in fragments shorter than    2922 bp

# BthCI.ClaI (GCNGC+ATCGAT) double digest, 6295 thousand fragments (6209929+86477 sites), 473 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     357 bp
  20% of sequence in fragments shorter than     457 bp
  25% of sequence in fragments shorter than     557 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     766 bp
  40% of sequence in fragments shorter than     871 bp
  45% of sequence in fragments shorter than     989 bp
  50% of sequence in fragments shorter than    1110 bp
  55% of sequence in fragments shorter than    1246 bp
  60% of sequence in fragments shorter than    1393 bp
  65% of sequence in fragments shorter than    1563 bp
  70% of sequence in fragments shorter than    1744 bp
  75% of sequence in fragments shorter than    1964 bp
  80% of sequence in fragments shorter than    2224 bp
  85% of sequence in fragments shorter than    2572 bp
  90% of sequence in fragments shorter than    3046 bp
  95% of sequence in fragments shorter than    3876 bp

# AvaI.BbvI (CYCGRG+GCAGC) double digest, 6295 thousand fragments (1005714+5290051 sites), 473 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     257 bp
  15% of sequence in fragments shorter than     341 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     519 bp
  30% of sequence in fragments shorter than     611 bp
  35% of sequence in fragments shorter than     707 bp
  40% of sequence in fragments shorter than     810 bp
  45% of sequence in fragments shorter than     913 bp
  50% of sequence in fragments shorter than    1030 bp
  55% of sequence in fragments shorter than    1156 bp
  60% of sequence in fragments shorter than    1294 bp
  65% of sequence in fragments shorter than    1452 bp
  70% of sequence in fragments shorter than    1626 bp
  75% of sequence in fragments shorter than    1829 bp
  80% of sequence in fragments shorter than    2073 bp
  85% of sequence in fragments shorter than    2405 bp
  90% of sequence in fragments shorter than    2877 bp
  95% of sequence in fragments shorter than    3692 bp

# HphI.KpnI (GGTGA+GGTACC) double digest, 6294 thousand fragments (6007336+287974 sites), 473 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     245 bp
  15% of sequence in fragments shorter than     319 bp
  20% of sequence in fragments shorter than     387 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     527 bp
  35% of sequence in fragments shorter than     594 bp
  40% of sequence in fragments shorter than     667 bp
  45% of sequence in fragments shorter than     740 bp
  50% of sequence in fragments shorter than     822 bp
  55% of sequence in fragments shorter than     910 bp
  60% of sequence in fragments shorter than    1010 bp
  65% of sequence in fragments shorter than    1119 bp
  70% of sequence in fragments shorter than    1243 bp
  75% of sequence in fragments shorter than    1379 bp
  80% of sequence in fragments shorter than    1551 bp
  85% of sequence in fragments shorter than    1775 bp
  90% of sequence in fragments shorter than    2082 bp
  95% of sequence in fragments shorter than    2643 bp

# HphI.NheI (GGTGA+GCTAGC) double digest, 6294 thousand fragments (6007336+287384 sites), 473 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     244 bp
  15% of sequence in fragments shorter than     319 bp
  20% of sequence in fragments shorter than     388 bp
  25% of sequence in fragments shorter than     458 bp
  30% of sequence in fragments shorter than     525 bp
  35% of sequence in fragments shorter than     593 bp
  40% of sequence in fragments shorter than     667 bp
  45% of sequence in fragments shorter than     744 bp
  50% of sequence in fragments shorter than     824 bp
  55% of sequence in fragments shorter than     907 bp
  60% of sequence in fragments shorter than    1005 bp
  65% of sequence in fragments shorter than    1111 bp
  70% of sequence in fragments shorter than    1230 bp
  75% of sequence in fragments shorter than    1373 bp
  80% of sequence in fragments shorter than    1539 bp
  85% of sequence in fragments shorter than    1760 bp
  90% of sequence in fragments shorter than    2066 bp
  95% of sequence in fragments shorter than    2620 bp

# BsrI.SphI (ACTGG+GCATGC) double digest, 6291 thousand fragments (5741310+550952 sites), 473 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     244 bp
  15% of sequence in fragments shorter than     319 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     602 bp
  40% of sequence in fragments shorter than     675 bp
  45% of sequence in fragments shorter than     744 bp
  50% of sequence in fragments shorter than     826 bp
  55% of sequence in fragments shorter than     911 bp
  60% of sequence in fragments shorter than    1008 bp
  65% of sequence in fragments shorter than    1113 bp
  70% of sequence in fragments shorter than    1234 bp
  75% of sequence in fragments shorter than    1377 bp
  80% of sequence in fragments shorter than    1545 bp
  85% of sequence in fragments shorter than    1762 bp
  90% of sequence in fragments shorter than    2069 bp
  95% of sequence in fragments shorter than    2626 bp

# BccI.XhoI (CCATC+CTCGAG) double digest, 6291 thousand fragments (6170927+121308 sites), 473 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     394 bp
  25% of sequence in fragments shorter than     463 bp
  30% of sequence in fragments shorter than     537 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     688 bp
  45% of sequence in fragments shorter than     770 bp
  50% of sequence in fragments shorter than     856 bp
  55% of sequence in fragments shorter than     951 bp
  60% of sequence in fragments shorter than    1054 bp
  65% of sequence in fragments shorter than    1164 bp
  70% of sequence in fragments shorter than    1293 bp
  75% of sequence in fragments shorter than    1449 bp
  80% of sequence in fragments shorter than    1638 bp
  85% of sequence in fragments shorter than    1883 bp
  90% of sequence in fragments shorter than    2241 bp
  95% of sequence in fragments shorter than    2908 bp

# FokI.PpuMI (GGATG+RGGWCCY) double digest, 6285 thousand fragments (5201116+1085138 sites), 474 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     248 bp
  15% of sequence in fragments shorter than     326 bp
  20% of sequence in fragments shorter than     399 bp
  25% of sequence in fragments shorter than     475 bp
  30% of sequence in fragments shorter than     553 bp
  35% of sequence in fragments shorter than     631 bp
  40% of sequence in fragments shorter than     713 bp
  45% of sequence in fragments shorter than     799 bp
  50% of sequence in fragments shorter than     892 bp
  55% of sequence in fragments shorter than     987 bp
  60% of sequence in fragments shorter than    1090 bp
  65% of sequence in fragments shorter than    1207 bp
  70% of sequence in fragments shorter than    1342 bp
  75% of sequence in fragments shorter than    1493 bp
  80% of sequence in fragments shorter than    1686 bp
  85% of sequence in fragments shorter than    1929 bp
  90% of sequence in fragments shorter than    2284 bp
  95% of sequence in fragments shorter than    2918 bp

# FokI.PvuII (GGATG+CAGCTG) double digest, 6284 thousand fragments (5201116+1084260 sites), 474 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     326 bp
  20% of sequence in fragments shorter than     400 bp
  25% of sequence in fragments shorter than     474 bp
  30% of sequence in fragments shorter than     550 bp
  35% of sequence in fragments shorter than     627 bp
  40% of sequence in fragments shorter than     708 bp
  45% of sequence in fragments shorter than     793 bp
  50% of sequence in fragments shorter than     883 bp
  55% of sequence in fragments shorter than     976 bp
  60% of sequence in fragments shorter than    1079 bp
  65% of sequence in fragments shorter than    1193 bp
  70% of sequence in fragments shorter than    1324 bp
  75% of sequence in fragments shorter than    1468 bp
  80% of sequence in fragments shorter than    1653 bp
  85% of sequence in fragments shorter than    1887 bp
  90% of sequence in fragments shorter than    2229 bp
  95% of sequence in fragments shorter than    2832 bp

# BsrI.ScaI (ACTGG+AGTACT) double digest, 6284 thousand fragments (5741310+543793 sites), 474 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     248 bp
  15% of sequence in fragments shorter than     324 bp
  20% of sequence in fragments shorter than     397 bp
  25% of sequence in fragments shorter than     467 bp
  30% of sequence in fragments shorter than     537 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     684 bp
  45% of sequence in fragments shorter than     753 bp
  50% of sequence in fragments shorter than     836 bp
  55% of sequence in fragments shorter than     921 bp
  60% of sequence in fragments shorter than    1019 bp
  65% of sequence in fragments shorter than    1124 bp
  70% of sequence in fragments shorter than    1246 bp
  75% of sequence in fragments shorter than    1386 bp
  80% of sequence in fragments shorter than    1555 bp
  85% of sequence in fragments shorter than    1771 bp
  90% of sequence in fragments shorter than    2076 bp
  95% of sequence in fragments shorter than    2629 bp

# AatII.BthCI (GACGTC+GCNGC) double digest, 6280 thousand fragments (71089+6209929 sites), 474 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     359 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     561 bp
  30% of sequence in fragments shorter than     664 bp
  35% of sequence in fragments shorter than     772 bp
  40% of sequence in fragments shorter than     878 bp
  45% of sequence in fragments shorter than     997 bp
  50% of sequence in fragments shorter than    1121 bp
  55% of sequence in fragments shorter than    1258 bp
  60% of sequence in fragments shorter than    1408 bp
  65% of sequence in fragments shorter than    1581 bp
  70% of sequence in fragments shorter than    1767 bp
  75% of sequence in fragments shorter than    1991 bp
  80% of sequence in fragments shorter than    2256 bp
  85% of sequence in fragments shorter than    2611 bp
  90% of sequence in fragments shorter than    3095 bp
  95% of sequence in fragments shorter than    3948 bp

# AvaII.SfeI (GGWCC+CTRYAG) double digest, 6278 thousand fragments (2747690+3531009 sites), 474 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     248 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     392 bp
  25% of sequence in fragments shorter than     466 bp
  30% of sequence in fragments shorter than     540 bp
  35% of sequence in fragments shorter than     618 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     780 bp
  50% of sequence in fragments shorter than     869 bp
  55% of sequence in fragments shorter than     956 bp
  60% of sequence in fragments shorter than    1060 bp
  65% of sequence in fragments shorter than    1175 bp
  70% of sequence in fragments shorter than    1306 bp
  75% of sequence in fragments shorter than    1455 bp
  80% of sequence in fragments shorter than    1642 bp
  85% of sequence in fragments shorter than    1873 bp
  90% of sequence in fragments shorter than    2203 bp
  95% of sequence in fragments shorter than    2776 bp

# BthCI.SacII (GCNGC+CCGCGG) double digest, 6276 thousand fragments (6209929+66901 sites), 474 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     362 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     672 bp
  35% of sequence in fragments shorter than     781 bp
  40% of sequence in fragments shorter than     888 bp
  45% of sequence in fragments shorter than    1009 bp
  50% of sequence in fragments shorter than    1134 bp
  55% of sequence in fragments shorter than    1273 bp
  60% of sequence in fragments shorter than    1425 bp
  65% of sequence in fragments shorter than    1599 bp
  70% of sequence in fragments shorter than    1786 bp
  75% of sequence in fragments shorter than    2015 bp
  80% of sequence in fragments shorter than    2282 bp
  85% of sequence in fragments shorter than    2643 bp
  90% of sequence in fragments shorter than    3132 bp
  95% of sequence in fragments shorter than    3993 bp

# BthCI.PspXI (GCNGC+VCTCGAGB) double digest, 6268 thousand fragments (6209929+59193 sites), 475 bp average fragment length
   5% of sequence in fragments shorter than     157 bp
  10% of sequence in fragments shorter than     264 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     461 bp
  25% of sequence in fragments shorter than     563 bp
  30% of sequence in fragments shorter than     666 bp
  35% of sequence in fragments shorter than     774 bp
  40% of sequence in fragments shorter than     881 bp
  45% of sequence in fragments shorter than    1000 bp
  50% of sequence in fragments shorter than    1124 bp
  55% of sequence in fragments shorter than    1263 bp
  60% of sequence in fragments shorter than    1413 bp
  65% of sequence in fragments shorter than    1587 bp
  70% of sequence in fragments shorter than    1771 bp
  75% of sequence in fragments shorter than    2000 bp
  80% of sequence in fragments shorter than    2265 bp
  85% of sequence in fragments shorter than    2622 bp
  90% of sequence in fragments shorter than    3108 bp
  95% of sequence in fragments shorter than    3962 bp

# BbvI.BspHI (GCAGC+TCATGA) double digest, 6267 thousand fragments (5290051+978290 sites), 475 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     338 bp
  20% of sequence in fragments shorter than     421 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     590 bp
  35% of sequence in fragments shorter than     673 bp
  40% of sequence in fragments shorter than     762 bp
  45% of sequence in fragments shorter than     856 bp
  50% of sequence in fragments shorter than     956 bp
  55% of sequence in fragments shorter than    1062 bp
  60% of sequence in fragments shorter than    1186 bp
  65% of sequence in fragments shorter than    1321 bp
  70% of sequence in fragments shorter than    1473 bp
  75% of sequence in fragments shorter than    1654 bp
  80% of sequence in fragments shorter than    1869 bp
  85% of sequence in fragments shorter than    2136 bp
  90% of sequence in fragments shorter than    2532 bp
  95% of sequence in fragments shorter than    3192 bp

# AgeI.BthCI (ACCGGT+GCNGC) double digest, 6262 thousand fragments (53386+6209929 sites), 475 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     264 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     461 bp
  25% of sequence in fragments shorter than     563 bp
  30% of sequence in fragments shorter than     666 bp
  35% of sequence in fragments shorter than     774 bp
  40% of sequence in fragments shorter than     880 bp
  45% of sequence in fragments shorter than    1000 bp
  50% of sequence in fragments shorter than    1124 bp
  55% of sequence in fragments shorter than    1262 bp
  60% of sequence in fragments shorter than    1412 bp
  65% of sequence in fragments shorter than    1585 bp
  70% of sequence in fragments shorter than    1769 bp
  75% of sequence in fragments shorter than    1993 bp
  80% of sequence in fragments shorter than    2261 bp
  85% of sequence in fragments shorter than    2619 bp
  90% of sequence in fragments shorter than    3104 bp
  95% of sequence in fragments shorter than    3956 bp

# SfeI.SmlI (CTRYAG+CTYRAG) double digest, 6258 thousand fragments (3531009+2727867 sites), 476 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     323 bp
  20% of sequence in fragments shorter than     394 bp
  25% of sequence in fragments shorter than     463 bp
  30% of sequence in fragments shorter than     531 bp
  35% of sequence in fragments shorter than     603 bp
  40% of sequence in fragments shorter than     675 bp
  45% of sequence in fragments shorter than     750 bp
  50% of sequence in fragments shorter than     832 bp
  55% of sequence in fragments shorter than     919 bp
  60% of sequence in fragments shorter than    1012 bp
  65% of sequence in fragments shorter than    1116 bp
  70% of sequence in fragments shorter than    1237 bp
  75% of sequence in fragments shorter than    1376 bp
  80% of sequence in fragments shorter than    1540 bp
  85% of sequence in fragments shorter than    1735 bp
  90% of sequence in fragments shorter than    2024 bp
  95% of sequence in fragments shorter than    2527 bp

# BccI.ClaI (CCATC+ATCGAT) double digest, 6256 thousand fragments (6170927+86477 sites), 476 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     248 bp
  15% of sequence in fragments shorter than     324 bp
  20% of sequence in fragments shorter than     396 bp
  25% of sequence in fragments shorter than     466 bp
  30% of sequence in fragments shorter than     540 bp
  35% of sequence in fragments shorter than     616 bp
  40% of sequence in fragments shorter than     692 bp
  45% of sequence in fragments shorter than     774 bp
  50% of sequence in fragments shorter than     860 bp
  55% of sequence in fragments shorter than     954 bp
  60% of sequence in fragments shorter than    1058 bp
  65% of sequence in fragments shorter than    1167 bp
  70% of sequence in fragments shorter than    1296 bp
  75% of sequence in fragments shorter than    1451 bp
  80% of sequence in fragments shorter than    1639 bp
  85% of sequence in fragments shorter than    1884 bp
  90% of sequence in fragments shorter than    2239 bp
  95% of sequence in fragments shorter than    2902 bp

# BthCI.PmeI (GCNGC+GTTTAAAC) double digest, 6251 thousand fragments (6209929+42094 sites), 476 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     461 bp
  25% of sequence in fragments shorter than     563 bp
  30% of sequence in fragments shorter than     666 bp
  35% of sequence in fragments shorter than     773 bp
  40% of sequence in fragments shorter than     879 bp
  45% of sequence in fragments shorter than     998 bp
  50% of sequence in fragments shorter than    1121 bp
  55% of sequence in fragments shorter than    1258 bp
  60% of sequence in fragments shorter than    1408 bp
  65% of sequence in fragments shorter than    1580 bp
  70% of sequence in fragments shorter than    1765 bp
  75% of sequence in fragments shorter than    1988 bp
  80% of sequence in fragments shorter than    2251 bp
  85% of sequence in fragments shorter than    2604 bp
  90% of sequence in fragments shorter than    3085 bp
  95% of sequence in fragments shorter than    3933 bp

# HpaII.MlyI (CCGG+GAGTC) double digest, 6248 thousand fragments (2317722+3931692 sites), 476 bp average fragment length
   5% of sequence in fragments shorter than     145 bp
  10% of sequence in fragments shorter than     241 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     491 bp
  30% of sequence in fragments shorter than     577 bp
  35% of sequence in fragments shorter than     666 bp
  40% of sequence in fragments shorter than     760 bp
  45% of sequence in fragments shorter than     860 bp
  50% of sequence in fragments shorter than     966 bp
  55% of sequence in fragments shorter than    1082 bp
  60% of sequence in fragments shorter than    1212 bp
  65% of sequence in fragments shorter than    1360 bp
  70% of sequence in fragments shorter than    1526 bp
  75% of sequence in fragments shorter than    1726 bp
  80% of sequence in fragments shorter than    1975 bp
  85% of sequence in fragments shorter than    2303 bp
  90% of sequence in fragments shorter than    2791 bp
  95% of sequence in fragments shorter than    3684 bp

# BsrBI.HphI (CCGCTC+GGTGA) double digest, 6242 thousand fragments (235523+6007336 sites), 477 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     246 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     463 bp
  30% of sequence in fragments shorter than     533 bp
  35% of sequence in fragments shorter than     604 bp
  40% of sequence in fragments shorter than     680 bp
  45% of sequence in fragments shorter than     758 bp
  50% of sequence in fragments shorter than     840 bp
  55% of sequence in fragments shorter than     930 bp
  60% of sequence in fragments shorter than    1033 bp
  65% of sequence in fragments shorter than    1145 bp
  70% of sequence in fragments shorter than    1273 bp
  75% of sequence in fragments shorter than    1412 bp
  80% of sequence in fragments shorter than    1594 bp
  85% of sequence in fragments shorter than    1833 bp
  90% of sequence in fragments shorter than    2156 bp
  95% of sequence in fragments shorter than    2730 bp

# AatII.BccI (GACGTC+CCATC) double digest, 6241 thousand fragments (71089+6170927 sites), 477 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     325 bp
  20% of sequence in fragments shorter than     397 bp
  25% of sequence in fragments shorter than     467 bp
  30% of sequence in fragments shorter than     541 bp
  35% of sequence in fragments shorter than     617 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     775 bp
  50% of sequence in fragments shorter than     862 bp
  55% of sequence in fragments shorter than     957 bp
  60% of sequence in fragments shorter than    1061 bp
  65% of sequence in fragments shorter than    1171 bp
  70% of sequence in fragments shorter than    1302 bp
  75% of sequence in fragments shorter than    1458 bp
  80% of sequence in fragments shorter than    1648 bp
  85% of sequence in fragments shorter than    1894 bp
  90% of sequence in fragments shorter than    2252 bp
  95% of sequence in fragments shorter than    2923 bp

# BthCI.SalI (GCNGC+GTCGAC) double digest, 6240 thousand fragments (6209929+31396 sites), 477 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     361 bp
  20% of sequence in fragments shorter than     463 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     669 bp
  35% of sequence in fragments shorter than     777 bp
  40% of sequence in fragments shorter than     884 bp
  45% of sequence in fragments shorter than    1004 bp
  50% of sequence in fragments shorter than    1128 bp
  55% of sequence in fragments shorter than    1266 bp
  60% of sequence in fragments shorter than    1418 bp
  65% of sequence in fragments shorter than    1591 bp
  70% of sequence in fragments shorter than    1776 bp
  75% of sequence in fragments shorter than    2004 bp
  80% of sequence in fragments shorter than    2269 bp
  85% of sequence in fragments shorter than    2628 bp
  90% of sequence in fragments shorter than    3114 bp
  95% of sequence in fragments shorter than    3971 bp

# BccI.SacII (CCATC+CCGCGG) double digest, 6237 thousand fragments (6170927+66901 sites), 477 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     325 bp
  20% of sequence in fragments shorter than     397 bp
  25% of sequence in fragments shorter than     467 bp
  30% of sequence in fragments shorter than     541 bp
  35% of sequence in fragments shorter than     618 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     777 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     959 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1174 bp
  70% of sequence in fragments shorter than    1305 bp
  75% of sequence in fragments shorter than    1462 bp
  80% of sequence in fragments shorter than    1652 bp
  85% of sequence in fragments shorter than    1900 bp
  90% of sequence in fragments shorter than    2261 bp
  95% of sequence in fragments shorter than    2937 bp

# HphI.KasI (GGTGA+GGCGCC) double digest, 6237 thousand fragments (6007336+230381 sites), 477 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     246 bp
  15% of sequence in fragments shorter than     323 bp
  20% of sequence in fragments shorter than     394 bp
  25% of sequence in fragments shorter than     466 bp
  30% of sequence in fragments shorter than     537 bp
  35% of sequence in fragments shorter than     609 bp
  40% of sequence in fragments shorter than     685 bp
  45% of sequence in fragments shorter than     765 bp
  50% of sequence in fragments shorter than     848 bp
  55% of sequence in fragments shorter than     941 bp
  60% of sequence in fragments shorter than    1043 bp
  65% of sequence in fragments shorter than    1158 bp
  70% of sequence in fragments shorter than    1288 bp
  75% of sequence in fragments shorter than    1425 bp
  80% of sequence in fragments shorter than    1609 bp
  85% of sequence in fragments shorter than    1850 bp
  90% of sequence in fragments shorter than    2178 bp
  95% of sequence in fragments shorter than    2764 bp

# ApaLI.BsrI (GTGCAC+ACTGG) double digest, 6237 thousand fragments (496255+5741310 sites), 477 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     323 bp
  20% of sequence in fragments shorter than     395 bp
  25% of sequence in fragments shorter than     465 bp
  30% of sequence in fragments shorter than     536 bp
  35% of sequence in fragments shorter than     610 bp
  40% of sequence in fragments shorter than     684 bp
  45% of sequence in fragments shorter than     755 bp
  50% of sequence in fragments shorter than     839 bp
  55% of sequence in fragments shorter than     927 bp
  60% of sequence in fragments shorter than    1027 bp
  65% of sequence in fragments shorter than    1134 bp
  70% of sequence in fragments shorter than    1258 bp
  75% of sequence in fragments shorter than    1402 bp
  80% of sequence in fragments shorter than    1575 bp
  85% of sequence in fragments shorter than    1796 bp
  90% of sequence in fragments shorter than    2108 bp
  95% of sequence in fragments shorter than    2675 bp

# HphI.SwaI (GGTGA+ATTTAAAT) double digest, 6233 thousand fragments (6007336+226602 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     322 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     461 bp
  30% of sequence in fragments shorter than     530 bp
  35% of sequence in fragments shorter than     599 bp
  40% of sequence in fragments shorter than     672 bp
  45% of sequence in fragments shorter than     748 bp
  50% of sequence in fragments shorter than     826 bp
  55% of sequence in fragments shorter than     914 bp
  60% of sequence in fragments shorter than    1012 bp
  65% of sequence in fragments shorter than    1120 bp
  70% of sequence in fragments shorter than    1242 bp
  75% of sequence in fragments shorter than    1379 bp
  80% of sequence in fragments shorter than    1543 bp
  85% of sequence in fragments shorter than    1766 bp
  90% of sequence in fragments shorter than    2072 bp
  95% of sequence in fragments shorter than    2611 bp

# BthCI.MluI (GCNGC+ACGCGT) double digest, 6230 thousand fragments (6209929+21415 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     158 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     362 bp
  20% of sequence in fragments shorter than     464 bp
  25% of sequence in fragments shorter than     566 bp
  30% of sequence in fragments shorter than     671 bp
  35% of sequence in fragments shorter than     779 bp
  40% of sequence in fragments shorter than     886 bp
  45% of sequence in fragments shorter than    1007 bp
  50% of sequence in fragments shorter than    1131 bp
  55% of sequence in fragments shorter than    1270 bp
  60% of sequence in fragments shorter than    1422 bp
  65% of sequence in fragments shorter than    1596 bp
  70% of sequence in fragments shorter than    1781 bp
  75% of sequence in fragments shorter than    2011 bp
  80% of sequence in fragments shorter than    2277 bp
  85% of sequence in fragments shorter than    2637 bp
  90% of sequence in fragments shorter than    3126 bp
  95% of sequence in fragments shorter than    3986 bp

# BccI.PspXI (CCATC+VCTCGAGB) double digest, 6229 thousand fragments (6170927+59193 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     325 bp
  20% of sequence in fragments shorter than     398 bp
  25% of sequence in fragments shorter than     468 bp
  30% of sequence in fragments shorter than     542 bp
  35% of sequence in fragments shorter than     618 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     777 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     959 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1174 bp
  70% of sequence in fragments shorter than    1304 bp
  75% of sequence in fragments shorter than    1461 bp
  80% of sequence in fragments shorter than    1651 bp
  85% of sequence in fragments shorter than    1898 bp
  90% of sequence in fragments shorter than    2258 bp
  95% of sequence in fragments shorter than    2931 bp

# BthCI.SgrAI (GCNGC+CRCCGGYG) double digest, 6224 thousand fragments (6209929+15270 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     363 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     672 bp
  35% of sequence in fragments shorter than     781 bp
  40% of sequence in fragments shorter than     888 bp
  45% of sequence in fragments shorter than    1009 bp
  50% of sequence in fragments shorter than    1134 bp
  55% of sequence in fragments shorter than    1273 bp
  60% of sequence in fragments shorter than    1425 bp
  65% of sequence in fragments shorter than    1599 bp
  70% of sequence in fragments shorter than    1785 bp
  75% of sequence in fragments shorter than    2015 bp
  80% of sequence in fragments shorter than    2282 bp
  85% of sequence in fragments shorter than    2643 bp
  90% of sequence in fragments shorter than    3132 bp
  95% of sequence in fragments shorter than    3993 bp

# BthCI.NruI (GCNGC+TCGCGA) double digest, 6224 thousand fragments (6209929+14872 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     362 bp
  20% of sequence in fragments shorter than     464 bp
  25% of sequence in fragments shorter than     567 bp
  30% of sequence in fragments shorter than     671 bp
  35% of sequence in fragments shorter than     780 bp
  40% of sequence in fragments shorter than     887 bp
  45% of sequence in fragments shorter than    1008 bp
  50% of sequence in fragments shorter than    1133 bp
  55% of sequence in fragments shorter than    1271 bp
  60% of sequence in fragments shorter than    1423 bp
  65% of sequence in fragments shorter than    1597 bp
  70% of sequence in fragments shorter than    1783 bp
  75% of sequence in fragments shorter than    2012 bp
  80% of sequence in fragments shorter than    2278 bp
  85% of sequence in fragments shorter than    2639 bp
  90% of sequence in fragments shorter than    3127 bp
  95% of sequence in fragments shorter than    3987 bp

# AgeI.BccI (ACCGGT+CCATC) double digest, 6223 thousand fragments (53386+6170927 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     325 bp
  20% of sequence in fragments shorter than     398 bp
  25% of sequence in fragments shorter than     469 bp
  30% of sequence in fragments shorter than     542 bp
  35% of sequence in fragments shorter than     619 bp
  40% of sequence in fragments shorter than     695 bp
  45% of sequence in fragments shorter than     777 bp
  50% of sequence in fragments shorter than     865 bp
  55% of sequence in fragments shorter than     960 bp
  60% of sequence in fragments shorter than    1064 bp
  65% of sequence in fragments shorter than    1175 bp
  70% of sequence in fragments shorter than    1305 bp
  75% of sequence in fragments shorter than    1461 bp
  80% of sequence in fragments shorter than    1652 bp
  85% of sequence in fragments shorter than    1899 bp
  90% of sequence in fragments shorter than    2257 bp
  95% of sequence in fragments shorter than    2930 bp

# BthCI.PvuI (GCNGC+CGATCG) double digest, 6223 thousand fragments (6209929+14186 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     362 bp
  20% of sequence in fragments shorter than     464 bp
  25% of sequence in fragments shorter than     567 bp
  30% of sequence in fragments shorter than     672 bp
  35% of sequence in fragments shorter than     780 bp
  40% of sequence in fragments shorter than     887 bp
  45% of sequence in fragments shorter than    1008 bp
  50% of sequence in fragments shorter than    1133 bp
  55% of sequence in fragments shorter than    1272 bp
  60% of sequence in fragments shorter than    1424 bp
  65% of sequence in fragments shorter than    1598 bp
  70% of sequence in fragments shorter than    1784 bp
  75% of sequence in fragments shorter than    2014 bp
  80% of sequence in fragments shorter than    2280 bp
  85% of sequence in fragments shorter than    2641 bp
  90% of sequence in fragments shorter than    3129 bp
  95% of sequence in fragments shorter than    3990 bp

# BthCI.FseI (GCNGC+GGCCGGCC) double digest, 6223 thousand fragments (6209929+13662 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     363 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     672 bp
  35% of sequence in fragments shorter than     781 bp
  40% of sequence in fragments shorter than     888 bp
  45% of sequence in fragments shorter than    1009 bp
  50% of sequence in fragments shorter than    1134 bp
  55% of sequence in fragments shorter than    1273 bp
  60% of sequence in fragments shorter than    1426 bp
  65% of sequence in fragments shorter than    1600 bp
  70% of sequence in fragments shorter than    1786 bp
  75% of sequence in fragments shorter than    2016 bp
  80% of sequence in fragments shorter than    2282 bp
  85% of sequence in fragments shorter than    2644 bp
  90% of sequence in fragments shorter than    3133 bp
  95% of sequence in fragments shorter than    3994 bp

# AvaIII.BbvI (ATGCAT+GCAGC) double digest, 6222 thousand fragments (933357+5290051 sites), 478 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     258 bp
  15% of sequence in fragments shorter than     341 bp
  20% of sequence in fragments shorter than     425 bp
  25% of sequence in fragments shorter than     509 bp
  30% of sequence in fragments shorter than     595 bp
  35% of sequence in fragments shorter than     684 bp
  40% of sequence in fragments shorter than     776 bp
  45% of sequence in fragments shorter than     868 bp
  50% of sequence in fragments shorter than     972 bp
  55% of sequence in fragments shorter than    1083 bp
  60% of sequence in fragments shorter than    1208 bp
  65% of sequence in fragments shorter than    1345 bp
  70% of sequence in fragments shorter than    1501 bp
  75% of sequence in fragments shorter than    1671 bp
  80% of sequence in fragments shorter than    1890 bp
  85% of sequence in fragments shorter than    2156 bp
  90% of sequence in fragments shorter than    2551 bp
  95% of sequence in fragments shorter than    3209 bp

# BthCI.NotI (GCNGC+GCGGCCGC) double digest, 6219 thousand fragments (6209929+9795 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     363 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     673 bp
  35% of sequence in fragments shorter than     782 bp
  40% of sequence in fragments shorter than     889 bp
  45% of sequence in fragments shorter than    1010 bp
  50% of sequence in fragments shorter than    1135 bp
  55% of sequence in fragments shorter than    1274 bp
  60% of sequence in fragments shorter than    1427 bp
  65% of sequence in fragments shorter than    1601 bp
  70% of sequence in fragments shorter than    1787 bp
  75% of sequence in fragments shorter than    2017 bp
  80% of sequence in fragments shorter than    2284 bp
  85% of sequence in fragments shorter than    2645 bp
  90% of sequence in fragments shorter than    3134 bp
  95% of sequence in fragments shorter than    3996 bp

# BthCI.RsrII (GCNGC+CGGWCCG) double digest, 6219 thousand fragments (6209929+9764 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     363 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     672 bp
  35% of sequence in fragments shorter than     781 bp
  40% of sequence in fragments shorter than     889 bp
  45% of sequence in fragments shorter than    1010 bp
  50% of sequence in fragments shorter than    1134 bp
  55% of sequence in fragments shorter than    1274 bp
  60% of sequence in fragments shorter than    1426 bp
  65% of sequence in fragments shorter than    1600 bp
  70% of sequence in fragments shorter than    1786 bp
  75% of sequence in fragments shorter than    2016 bp
  80% of sequence in fragments shorter than    2283 bp
  85% of sequence in fragments shorter than    2644 bp
  90% of sequence in fragments shorter than    3133 bp
  95% of sequence in fragments shorter than    3995 bp

# NspI.StyI (RCATGY+CCWWGG) double digest, 6217 thousand fragments (3104359+3114107 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     327 bp
  20% of sequence in fragments shorter than     399 bp
  25% of sequence in fragments shorter than     471 bp
  30% of sequence in fragments shorter than     541 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     683 bp
  45% of sequence in fragments shorter than     761 bp
  50% of sequence in fragments shorter than     846 bp
  55% of sequence in fragments shorter than     936 bp
  60% of sequence in fragments shorter than    1035 bp
  65% of sequence in fragments shorter than    1144 bp
  70% of sequence in fragments shorter than    1270 bp
  75% of sequence in fragments shorter than    1415 bp
  80% of sequence in fragments shorter than    1595 bp
  85% of sequence in fragments shorter than    1827 bp
  90% of sequence in fragments shorter than    2160 bp
  95% of sequence in fragments shorter than    2761 bp

# AccI.CviQI (GTMKAC+GTAC) double digest, 6215 thousand fragments (1101063+5115078 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     255 bp
  15% of sequence in fragments shorter than     333 bp
  20% of sequence in fragments shorter than     407 bp
  25% of sequence in fragments shorter than     477 bp
  30% of sequence in fragments shorter than     545 bp
  35% of sequence in fragments shorter than     619 bp
  40% of sequence in fragments shorter than     691 bp
  45% of sequence in fragments shorter than     768 bp
  50% of sequence in fragments shorter than     851 bp
  55% of sequence in fragments shorter than     938 bp
  60% of sequence in fragments shorter than    1033 bp
  65% of sequence in fragments shorter than    1140 bp
  70% of sequence in fragments shorter than    1256 bp
  75% of sequence in fragments shorter than    1382 bp
  80% of sequence in fragments shorter than    1550 bp
  85% of sequence in fragments shorter than    1764 bp
  90% of sequence in fragments shorter than    2073 bp
  95% of sequence in fragments shorter than    2619 bp

# BbvI.StuI (GCAGC+AGGCCT) double digest, 6215 thousand fragments (5290051+925729 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     156 bp
  10% of sequence in fragments shorter than     254 bp
  15% of sequence in fragments shorter than     341 bp
  20% of sequence in fragments shorter than     425 bp
  25% of sequence in fragments shorter than     518 bp
  30% of sequence in fragments shorter than     612 bp
  35% of sequence in fragments shorter than     701 bp
  40% of sequence in fragments shorter than     804 bp
  45% of sequence in fragments shorter than     909 bp
  50% of sequence in fragments shorter than    1025 bp
  55% of sequence in fragments shorter than    1151 bp
  60% of sequence in fragments shorter than    1292 bp
  65% of sequence in fragments shorter than    1449 bp
  70% of sequence in fragments shorter than    1622 bp
  75% of sequence in fragments shorter than    1820 bp
  80% of sequence in fragments shorter than    2060 bp
  85% of sequence in fragments shorter than    2381 bp
  90% of sequence in fragments shorter than    2830 bp
  95% of sequence in fragments shorter than    3601 bp

# BscGI.TfiI (CCCGT+GAWTC) double digest, 6214 thousand fragments (1231070+4984362 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     319 bp
  20% of sequence in fragments shorter than     385 bp
  25% of sequence in fragments shorter than     459 bp
  30% of sequence in fragments shorter than     531 bp
  35% of sequence in fragments shorter than     607 bp
  40% of sequence in fragments shorter than     681 bp
  45% of sequence in fragments shorter than     758 bp
  50% of sequence in fragments shorter than     837 bp
  55% of sequence in fragments shorter than     925 bp
  60% of sequence in fragments shorter than    1021 bp
  65% of sequence in fragments shorter than    1127 bp
  70% of sequence in fragments shorter than    1246 bp
  75% of sequence in fragments shorter than    1383 bp
  80% of sequence in fragments shorter than    1548 bp
  85% of sequence in fragments shorter than    1758 bp
  90% of sequence in fragments shorter than    2043 bp
  95% of sequence in fragments shorter than    2518 bp

# AscI.BthCI (GGCGCGCC+GCNGC) double digest, 6214 thousand fragments (4751+6209929 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     363 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     673 bp
  35% of sequence in fragments shorter than     782 bp
  40% of sequence in fragments shorter than     889 bp
  45% of sequence in fragments shorter than    1010 bp
  50% of sequence in fragments shorter than    1135 bp
  55% of sequence in fragments shorter than    1274 bp
  60% of sequence in fragments shorter than    1426 bp
  65% of sequence in fragments shorter than    1600 bp
  70% of sequence in fragments shorter than    1787 bp
  75% of sequence in fragments shorter than    2017 bp
  80% of sequence in fragments shorter than    2283 bp
  85% of sequence in fragments shorter than    2645 bp
  90% of sequence in fragments shorter than    3134 bp
  95% of sequence in fragments shorter than    3996 bp

# BccI.PmeI (CCATC+GTTTAAAC) double digest, 6212 thousand fragments (6170927+42094 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     250 bp
  15% of sequence in fragments shorter than     326 bp
  20% of sequence in fragments shorter than     399 bp
  25% of sequence in fragments shorter than     469 bp
  30% of sequence in fragments shorter than     543 bp
  35% of sequence in fragments shorter than     619 bp
  40% of sequence in fragments shorter than     695 bp
  45% of sequence in fragments shorter than     778 bp
  50% of sequence in fragments shorter than     865 bp
  55% of sequence in fragments shorter than     960 bp
  60% of sequence in fragments shorter than    1064 bp
  65% of sequence in fragments shorter than    1174 bp
  70% of sequence in fragments shorter than    1304 bp
  75% of sequence in fragments shorter than    1460 bp
  80% of sequence in fragments shorter than    1649 bp
  85% of sequence in fragments shorter than    1895 bp
  90% of sequence in fragments shorter than    2252 bp
  95% of sequence in fragments shorter than    2919 bp

# BbvI.BthCI (GCAGC+GCNGC) double digest, 6209 thousand fragments (5290051+6209929 sites), 479 bp average fragment length
   5% of sequence in fragments shorter than     159 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     363 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     673 bp
  35% of sequence in fragments shorter than     782 bp
  40% of sequence in fragments shorter than     889 bp
  45% of sequence in fragments shorter than    1010 bp
  50% of sequence in fragments shorter than    1135 bp
  55% of sequence in fragments shorter than    1274 bp
  60% of sequence in fragments shorter than    1427 bp
  65% of sequence in fragments shorter than    1601 bp
  70% of sequence in fragments shorter than    1787 bp
  75% of sequence in fragments shorter than    2017 bp
  80% of sequence in fragments shorter than    2284 bp
  85% of sequence in fragments shorter than    2645 bp
  90% of sequence in fragments shorter than    3134 bp
  95% of sequence in fragments shorter than    3996 bp

# AvaI.FokI (CYCGRG+GGATG) double digest, 6206 thousand fragments (1005714+5201116 sites), 480 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     252 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     405 bp
  25% of sequence in fragments shorter than     481 bp
  30% of sequence in fragments shorter than     558 bp
  35% of sequence in fragments shorter than     636 bp
  40% of sequence in fragments shorter than     717 bp
  45% of sequence in fragments shorter than     804 bp
  50% of sequence in fragments shorter than     897 bp
  55% of sequence in fragments shorter than     992 bp
  60% of sequence in fragments shorter than    1097 bp
  65% of sequence in fragments shorter than    1215 bp
  70% of sequence in fragments shorter than    1354 bp
  75% of sequence in fragments shorter than    1505 bp
  80% of sequence in fragments shorter than    1698 bp
  85% of sequence in fragments shorter than    1942 bp
  90% of sequence in fragments shorter than    2299 bp
  95% of sequence in fragments shorter than    2944 bp

# BccI.SalI (CCATC+GTCGAC) double digest, 6201 thousand fragments (6170927+31396 sites), 480 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     250 bp
  15% of sequence in fragments shorter than     327 bp
  20% of sequence in fragments shorter than     400 bp
  25% of sequence in fragments shorter than     470 bp
  30% of sequence in fragments shorter than     544 bp
  35% of sequence in fragments shorter than     620 bp
  40% of sequence in fragments shorter than     697 bp
  45% of sequence in fragments shorter than     780 bp
  50% of sequence in fragments shorter than     868 bp
  55% of sequence in fragments shorter than     963 bp
  60% of sequence in fragments shorter than    1067 bp
  65% of sequence in fragments shorter than    1178 bp
  70% of sequence in fragments shorter than    1309 bp
  75% of sequence in fragments shorter than    1466 bp
  80% of sequence in fragments shorter than    1657 bp
  85% of sequence in fragments shorter than    1904 bp
  90% of sequence in fragments shorter than    2265 bp
  95% of sequence in fragments shorter than    2938 bp

# ApaI.BsrI (GGGCCC+ACTGG) double digest, 6201 thousand fragments (460339+5741310 sites), 480 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     247 bp
  15% of sequence in fragments shorter than     325 bp
  20% of sequence in fragments shorter than     400 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     545 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     696 bp
  45% of sequence in fragments shorter than     772 bp
  50% of sequence in fragments shorter than     859 bp
  55% of sequence in fragments shorter than     951 bp
  60% of sequence in fragments shorter than    1052 bp
  65% of sequence in fragments shorter than    1167 bp
  70% of sequence in fragments shorter than    1297 bp
  75% of sequence in fragments shorter than    1446 bp
  80% of sequence in fragments shorter than    1629 bp
  85% of sequence in fragments shorter than    1866 bp
  90% of sequence in fragments shorter than    2196 bp
  95% of sequence in fragments shorter than    2790 bp

# CviQI.PpuMI (GTAC+RGGWCCY) double digest, 6199 thousand fragments (5115078+1085138 sites), 480 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     255 bp
  15% of sequence in fragments shorter than     332 bp
  20% of sequence in fragments shorter than     402 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     540 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     682 bp
  45% of sequence in fragments shorter than     758 bp
  50% of sequence in fragments shorter than     838 bp
  55% of sequence in fragments shorter than     921 bp
  60% of sequence in fragments shorter than    1015 bp
  65% of sequence in fragments shorter than    1117 bp
  70% of sequence in fragments shorter than    1232 bp
  75% of sequence in fragments shorter than    1359 bp
  80% of sequence in fragments shorter than    1516 bp
  85% of sequence in fragments shorter than    1721 bp
  90% of sequence in fragments shorter than    2017 bp
  95% of sequence in fragments shorter than    2532 bp

# CviQI.PvuII (GTAC+CAGCTG) double digest, 6198 thousand fragments (5115078+1084260 sites), 480 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     333 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     475 bp
  30% of sequence in fragments shorter than     543 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     684 bp
  45% of sequence in fragments shorter than     759 bp
  50% of sequence in fragments shorter than     839 bp
  55% of sequence in fragments shorter than     924 bp
  60% of sequence in fragments shorter than    1017 bp
  65% of sequence in fragments shorter than    1118 bp
  70% of sequence in fragments shorter than    1229 bp
  75% of sequence in fragments shorter than    1358 bp
  80% of sequence in fragments shorter than    1509 bp
  85% of sequence in fragments shorter than    1712 bp
  90% of sequence in fragments shorter than    2001 bp
  95% of sequence in fragments shorter than    2498 bp

# BbvI.NdeI (GCAGC+CATATG) double digest, 6192 thousand fragments (5290051+903360 sites), 481 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     260 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     429 bp
  25% of sequence in fragments shorter than     512 bp
  30% of sequence in fragments shorter than     601 bp
  35% of sequence in fragments shorter than     690 bp
  40% of sequence in fragments shorter than     782 bp
  45% of sequence in fragments shorter than     874 bp
  50% of sequence in fragments shorter than     975 bp
  55% of sequence in fragments shorter than    1086 bp
  60% of sequence in fragments shorter than    1208 bp
  65% of sequence in fragments shorter than    1344 bp
  70% of sequence in fragments shorter than    1499 bp
  75% of sequence in fragments shorter than    1681 bp
  80% of sequence in fragments shorter than    1898 bp
  85% of sequence in fragments shorter than    2166 bp
  90% of sequence in fragments shorter than    2562 bp
  95% of sequence in fragments shorter than    3219 bp

# BccI.MluI (CCATC+ACGCGT) double digest, 6191 thousand fragments (6170927+21415 sites), 481 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     327 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     471 bp
  30% of sequence in fragments shorter than     545 bp
  35% of sequence in fragments shorter than     621 bp
  40% of sequence in fragments shorter than     699 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     869 bp
  55% of sequence in fragments shorter than     965 bp
  60% of sequence in fragments shorter than    1069 bp
  65% of sequence in fragments shorter than    1181 bp
  70% of sequence in fragments shorter than    1312 bp
  75% of sequence in fragments shorter than    1469 bp
  80% of sequence in fragments shorter than    1661 bp
  85% of sequence in fragments shorter than    1908 bp
  90% of sequence in fragments shorter than    2270 bp
  95% of sequence in fragments shorter than    2944 bp

# BsrI.EcoRV (ACTGG+GATATC) double digest, 6187 thousand fragments (5741310+446473 sites), 481 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     325 bp
  20% of sequence in fragments shorter than     399 bp
  25% of sequence in fragments shorter than     466 bp
  30% of sequence in fragments shorter than     537 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     684 bp
  45% of sequence in fragments shorter than     757 bp
  50% of sequence in fragments shorter than     839 bp
  55% of sequence in fragments shorter than     928 bp
  60% of sequence in fragments shorter than    1026 bp
  65% of sequence in fragments shorter than    1131 bp
  70% of sequence in fragments shorter than    1253 bp
  75% of sequence in fragments shorter than    1393 bp
  80% of sequence in fragments shorter than    1564 bp
  85% of sequence in fragments shorter than    1782 bp
  90% of sequence in fragments shorter than    2087 bp
  95% of sequence in fragments shorter than    2627 bp

# BccI.SgrAI (CCATC+CRCCGGYG) double digest, 6185 thousand fragments (6170927+15270 sites), 481 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     471 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     699 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     966 bp
  60% of sequence in fragments shorter than    1070 bp
  65% of sequence in fragments shorter than    1182 bp
  70% of sequence in fragments shorter than    1313 bp
  75% of sequence in fragments shorter than    1470 bp
  80% of sequence in fragments shorter than    1662 bp
  85% of sequence in fragments shorter than    1910 bp
  90% of sequence in fragments shorter than    2272 bp
  95% of sequence in fragments shorter than    2948 bp

# BccI.NruI (CCATC+TCGCGA) double digest, 6185 thousand fragments (6170927+14872 sites), 481 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     966 bp
  60% of sequence in fragments shorter than    1070 bp
  65% of sequence in fragments shorter than    1182 bp
  70% of sequence in fragments shorter than    1313 bp
  75% of sequence in fragments shorter than    1471 bp
  80% of sequence in fragments shorter than    1662 bp
  85% of sequence in fragments shorter than    1910 bp
  90% of sequence in fragments shorter than    2272 bp
  95% of sequence in fragments shorter than    2948 bp

# BccI.PvuI (CCATC+CGATCG) double digest, 6184 thousand fragments (6170927+14186 sites), 481 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     871 bp
  55% of sequence in fragments shorter than     967 bp
  60% of sequence in fragments shorter than    1070 bp
  65% of sequence in fragments shorter than    1183 bp
  70% of sequence in fragments shorter than    1313 bp
  75% of sequence in fragments shorter than    1471 bp
  80% of sequence in fragments shorter than    1662 bp
  85% of sequence in fragments shorter than    1907 bp
  90% of sequence in fragments shorter than    2267 bp
  95% of sequence in fragments shorter than    2933 bp

# BccI.FseI (CCATC+GGCCGGCC) double digest, 6184 thousand fragments (6170927+13662 sites), 481 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     967 bp
  60% of sequence in fragments shorter than    1070 bp
  65% of sequence in fragments shorter than    1183 bp
  70% of sequence in fragments shorter than    1314 bp
  75% of sequence in fragments shorter than    1471 bp
  80% of sequence in fragments shorter than    1663 bp
  85% of sequence in fragments shorter than    1912 bp
  90% of sequence in fragments shorter than    2274 bp
  95% of sequence in fragments shorter than    2950 bp

# BccI.NotI (CCATC+GCGGCCGC) double digest, 6180 thousand fragments (6170927+9795 sites), 482 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     783 bp
  50% of sequence in fragments shorter than     871 bp
  55% of sequence in fragments shorter than     967 bp
  60% of sequence in fragments shorter than    1070 bp
  65% of sequence in fragments shorter than    1183 bp
  70% of sequence in fragments shorter than    1314 bp
  75% of sequence in fragments shorter than    1471 bp
  80% of sequence in fragments shorter than    1663 bp
  85% of sequence in fragments shorter than    1912 bp
  90% of sequence in fragments shorter than    2273 bp
  95% of sequence in fragments shorter than    2950 bp

# BccI.RsrII (CCATC+CGGWCCG) double digest, 6180 thousand fragments (6170927+9764 sites), 482 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     783 bp
  50% of sequence in fragments shorter than     871 bp
  55% of sequence in fragments shorter than     967 bp
  60% of sequence in fragments shorter than    1070 bp
  65% of sequence in fragments shorter than    1183 bp
  70% of sequence in fragments shorter than    1314 bp
  75% of sequence in fragments shorter than    1472 bp
  80% of sequence in fragments shorter than    1663 bp
  85% of sequence in fragments shorter than    1912 bp
  90% of sequence in fragments shorter than    2274 bp
  95% of sequence in fragments shorter than    2950 bp

# AclI.HphI (AACGTT+GGTGA) double digest, 6179 thousand fragments (172706+6007336 sites), 482 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     325 bp
  20% of sequence in fragments shorter than     394 bp
  25% of sequence in fragments shorter than     466 bp
  30% of sequence in fragments shorter than     536 bp
  35% of sequence in fragments shorter than     606 bp
  40% of sequence in fragments shorter than     681 bp
  45% of sequence in fragments shorter than     759 bp
  50% of sequence in fragments shorter than     840 bp
  55% of sequence in fragments shorter than     928 bp
  60% of sequence in fragments shorter than    1029 bp
  65% of sequence in fragments shorter than    1136 bp
  70% of sequence in fragments shorter than    1261 bp
  75% of sequence in fragments shorter than    1394 bp
  80% of sequence in fragments shorter than    1568 bp
  85% of sequence in fragments shorter than    1795 bp
  90% of sequence in fragments shorter than    2101 bp
  95% of sequence in fragments shorter than    2638 bp

# BspHI.FokI (TCATGA+GGATG) double digest, 6178 thousand fragments (978290+5201116 sites), 482 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     257 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     407 bp
  25% of sequence in fragments shorter than     481 bp
  30% of sequence in fragments shorter than     554 bp
  35% of sequence in fragments shorter than     629 bp
  40% of sequence in fragments shorter than     706 bp
  45% of sequence in fragments shorter than     786 bp
  50% of sequence in fragments shorter than     873 bp
  55% of sequence in fragments shorter than     961 bp
  60% of sequence in fragments shorter than    1060 bp
  65% of sequence in fragments shorter than    1169 bp
  70% of sequence in fragments shorter than    1292 bp
  75% of sequence in fragments shorter than    1428 bp
  80% of sequence in fragments shorter than    1603 bp
  85% of sequence in fragments shorter than    1824 bp
  90% of sequence in fragments shorter than    2144 bp
  95% of sequence in fragments shorter than    2718 bp

# AscI.BccI (GGCGCGCC+CCATC) double digest, 6175 thousand fragments (4751+6170927 sites), 482 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     252 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     402 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     547 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     701 bp
  45% of sequence in fragments shorter than     783 bp
  50% of sequence in fragments shorter than     872 bp
  55% of sequence in fragments shorter than     968 bp
  60% of sequence in fragments shorter than    1071 bp
  65% of sequence in fragments shorter than    1184 bp
  70% of sequence in fragments shorter than    1315 bp
  75% of sequence in fragments shorter than    1473 bp
  80% of sequence in fragments shorter than    1665 bp
  85% of sequence in fragments shorter than    1913 bp
  90% of sequence in fragments shorter than    2275 bp
  95% of sequence in fragments shorter than    2952 bp

# HphI.PacI (GGTGA+TTAATTAA) double digest, 6166 thousand fragments (6007336+159455 sites), 483 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     250 bp
  15% of sequence in fragments shorter than     326 bp
  20% of sequence in fragments shorter than     395 bp
  25% of sequence in fragments shorter than     467 bp
  30% of sequence in fragments shorter than     537 bp
  35% of sequence in fragments shorter than     607 bp
  40% of sequence in fragments shorter than     683 bp
  45% of sequence in fragments shorter than     759 bp
  50% of sequence in fragments shorter than     841 bp
  55% of sequence in fragments shorter than     930 bp
  60% of sequence in fragments shorter than    1031 bp
  65% of sequence in fragments shorter than    1141 bp
  70% of sequence in fragments shorter than    1266 bp
  75% of sequence in fragments shorter than    1400 bp
  80% of sequence in fragments shorter than    1576 bp
  85% of sequence in fragments shorter than    1808 bp
  90% of sequence in fragments shorter than    2123 bp
  95% of sequence in fragments shorter than    2680 bp

# HphI.Hpy99I (GGTGA+CGWCG) double digest, 6164 thousand fragments (6007336+157917 sites), 483 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     249 bp
  15% of sequence in fragments shorter than     326 bp
  20% of sequence in fragments shorter than     396 bp
  25% of sequence in fragments shorter than     468 bp
  30% of sequence in fragments shorter than     539 bp
  35% of sequence in fragments shorter than     611 bp
  40% of sequence in fragments shorter than     687 bp
  45% of sequence in fragments shorter than     766 bp
  50% of sequence in fragments shorter than     850 bp
  55% of sequence in fragments shorter than     941 bp
  60% of sequence in fragments shorter than    1043 bp
  65% of sequence in fragments shorter than    1158 bp
  70% of sequence in fragments shorter than    1287 bp
  75% of sequence in fragments shorter than    1424 bp
  80% of sequence in fragments shorter than    1607 bp
  85% of sequence in fragments shorter than    1848 bp
  90% of sequence in fragments shorter than    2175 bp
  95% of sequence in fragments shorter than    2759 bp

# BbvI.HindIII (GCAGC+AAGCTT) double digest, 6149 thousand fragments (5290051+860365 sites), 484 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     518 bp
  30% of sequence in fragments shorter than     606 bp
  35% of sequence in fragments shorter than     697 bp
  40% of sequence in fragments shorter than     794 bp
  45% of sequence in fragments shorter than     889 bp
  50% of sequence in fragments shorter than     995 bp
  55% of sequence in fragments shorter than    1109 bp
  60% of sequence in fragments shorter than    1236 bp
  65% of sequence in fragments shorter than    1378 bp
  70% of sequence in fragments shorter than    1541 bp
  75% of sequence in fragments shorter than    1717 bp
  80% of sequence in fragments shorter than    1925 bp
  85% of sequence in fragments shorter than    2206 bp
  90% of sequence in fragments shorter than    2609 bp
  95% of sequence in fragments shorter than    3305 bp

# BsrI.SpeI (ACTGG+ACTAGT) double digest, 6141 thousand fragments (5741310+400286 sites), 485 bp average fragment length
   5% of sequence in fragments shorter than     164 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     329 bp
  20% of sequence in fragments shorter than     403 bp
  25% of sequence in fragments shorter than     474 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     620 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     766 bp
  50% of sequence in fragments shorter than     849 bp
  55% of sequence in fragments shorter than     937 bp
  60% of sequence in fragments shorter than    1034 bp
  65% of sequence in fragments shorter than    1141 bp
  70% of sequence in fragments shorter than    1265 bp
  75% of sequence in fragments shorter than    1406 bp
  80% of sequence in fragments shorter than    1577 bp
  85% of sequence in fragments shorter than    1796 bp
  90% of sequence in fragments shorter than    2107 bp
  95% of sequence in fragments shorter than    2663 bp

# BbvI.XbaI (GCAGC+TCTAGA) double digest, 6140 thousand fragments (5290051+850998 sites), 485 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     258 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     516 bp
  30% of sequence in fragments shorter than     605 bp
  35% of sequence in fragments shorter than     696 bp
  40% of sequence in fragments shorter than     793 bp
  45% of sequence in fragments shorter than     889 bp
  50% of sequence in fragments shorter than     997 bp
  55% of sequence in fragments shorter than    1116 bp
  60% of sequence in fragments shorter than    1239 bp
  65% of sequence in fragments shorter than    1379 bp
  70% of sequence in fragments shorter than    1540 bp
  75% of sequence in fragments shorter than    1717 bp
  80% of sequence in fragments shorter than    1940 bp
  85% of sequence in fragments shorter than    2228 bp
  90% of sequence in fragments shorter than    2633 bp
  95% of sequence in fragments shorter than    3336 bp

# HphI.SnaBI (GGTGA+TACGTA) double digest, 6139 thousand fragments (6007336+132299 sites), 485 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     250 bp
  15% of sequence in fragments shorter than     327 bp
  20% of sequence in fragments shorter than     397 bp
  25% of sequence in fragments shorter than     469 bp
  30% of sequence in fragments shorter than     539 bp
  35% of sequence in fragments shorter than     610 bp
  40% of sequence in fragments shorter than     685 bp
  45% of sequence in fragments shorter than     763 bp
  50% of sequence in fragments shorter than     845 bp
  55% of sequence in fragments shorter than     935 bp
  60% of sequence in fragments shorter than    1036 bp
  65% of sequence in fragments shorter than    1148 bp
  70% of sequence in fragments shorter than    1274 bp
  75% of sequence in fragments shorter than    1409 bp
  80% of sequence in fragments shorter than    1587 bp
  85% of sequence in fragments shorter than    1821 bp
  90% of sequence in fragments shorter than    2139 bp
  95% of sequence in fragments shorter than    2705 bp

# BbvI.EcoRI (GCAGC+GAATTC) double digest, 6136 thousand fragments (5290051+847401 sites), 485 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     344 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     517 bp
  30% of sequence in fragments shorter than     605 bp
  35% of sequence in fragments shorter than     693 bp
  40% of sequence in fragments shorter than     789 bp
  45% of sequence in fragments shorter than     885 bp
  50% of sequence in fragments shorter than     992 bp
  55% of sequence in fragments shorter than    1106 bp
  60% of sequence in fragments shorter than    1232 bp
  65% of sequence in fragments shorter than    1371 bp
  70% of sequence in fragments shorter than    1533 bp
  75% of sequence in fragments shorter than    1704 bp
  80% of sequence in fragments shorter than    1917 bp
  85% of sequence in fragments shorter than    2191 bp
  90% of sequence in fragments shorter than    2585 bp
  95% of sequence in fragments shorter than    3250 bp

# AvaIII.FokI (ATGCAT+GGATG) double digest, 6133 thousand fragments (933357+5201116 sites), 485 bp average fragment length
   5% of sequence in fragments shorter than     170 bp
  10% of sequence in fragments shorter than     261 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     413 bp
  25% of sequence in fragments shorter than     488 bp
  30% of sequence in fragments shorter than     562 bp
  35% of sequence in fragments shorter than     637 bp
  40% of sequence in fragments shorter than     715 bp
  45% of sequence in fragments shorter than     797 bp
  50% of sequence in fragments shorter than     884 bp
  55% of sequence in fragments shorter than     973 bp
  60% of sequence in fragments shorter than    1073 bp
  65% of sequence in fragments shorter than    1181 bp
  70% of sequence in fragments shorter than    1306 bp
  75% of sequence in fragments shorter than    1442 bp
  80% of sequence in fragments shorter than    1616 bp
  85% of sequence in fragments shorter than    1834 bp
  90% of sequence in fragments shorter than    2153 bp
  95% of sequence in fragments shorter than    2716 bp

# HphI.NaeI (GGTGA+GCCGGC) double digest, 6132 thousand fragments (6007336+125692 sites), 485 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     250 bp
  15% of sequence in fragments shorter than     327 bp
  20% of sequence in fragments shorter than     398 bp
  25% of sequence in fragments shorter than     471 bp
  30% of sequence in fragments shorter than     542 bp
  35% of sequence in fragments shorter than     614 bp
  40% of sequence in fragments shorter than     690 bp
  45% of sequence in fragments shorter than     770 bp
  50% of sequence in fragments shorter than     854 bp
  55% of sequence in fragments shorter than     946 bp
  60% of sequence in fragments shorter than    1049 bp
  65% of sequence in fragments shorter than    1164 bp
  70% of sequence in fragments shorter than    1294 bp
  75% of sequence in fragments shorter than    1432 bp
  80% of sequence in fragments shorter than    1616 bp
  85% of sequence in fragments shorter than    1858 bp
  90% of sequence in fragments shorter than    2184 bp
  95% of sequence in fragments shorter than    2774 bp

# BsrI.HpaI (ACTGG+GTTAAC) double digest, 6130 thousand fragments (5741310+389418 sites), 486 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     252 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     403 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     544 bp
  35% of sequence in fragments shorter than     618 bp
  40% of sequence in fragments shorter than     692 bp
  45% of sequence in fragments shorter than     765 bp
  50% of sequence in fragments shorter than     849 bp
  55% of sequence in fragments shorter than     937 bp
  60% of sequence in fragments shorter than    1034 bp
  65% of sequence in fragments shorter than    1144 bp
  70% of sequence in fragments shorter than    1267 bp
  75% of sequence in fragments shorter than    1410 bp
  80% of sequence in fragments shorter than    1583 bp
  85% of sequence in fragments shorter than    1807 bp
  90% of sequence in fragments shorter than    2117 bp
  95% of sequence in fragments shorter than    2691 bp

# HphI.XhoI (GGTGA+CTCGAG) double digest, 6128 thousand fragments (6007336+121308 sites), 486 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     398 bp
  25% of sequence in fragments shorter than     471 bp
  30% of sequence in fragments shorter than     542 bp
  35% of sequence in fragments shorter than     614 bp
  40% of sequence in fragments shorter than     689 bp
  45% of sequence in fragments shorter than     769 bp
  50% of sequence in fragments shorter than     853 bp
  55% of sequence in fragments shorter than     945 bp
  60% of sequence in fragments shorter than    1046 bp
  65% of sequence in fragments shorter than    1161 bp
  70% of sequence in fragments shorter than    1290 bp
  75% of sequence in fragments shorter than    1427 bp
  80% of sequence in fragments shorter than    1610 bp
  85% of sequence in fragments shorter than    1850 bp
  90% of sequence in fragments shorter than    2176 bp
  95% of sequence in fragments shorter than    2758 bp

# FokI.StuI (GGATG+AGGCCT) double digest, 6126 thousand fragments (5201116+925729 sites), 486 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     257 bp
  15% of sequence in fragments shorter than     336 bp
  20% of sequence in fragments shorter than     405 bp
  25% of sequence in fragments shorter than     481 bp
  30% of sequence in fragments shorter than     557 bp
  35% of sequence in fragments shorter than     636 bp
  40% of sequence in fragments shorter than     711 bp
  45% of sequence in fragments shorter than     797 bp
  50% of sequence in fragments shorter than     886 bp
  55% of sequence in fragments shorter than     978 bp
  60% of sequence in fragments shorter than    1081 bp
  65% of sequence in fragments shorter than    1198 bp
  70% of sequence in fragments shorter than    1332 bp
  75% of sequence in fragments shorter than    1479 bp
  80% of sequence in fragments shorter than    1663 bp
  85% of sequence in fragments shorter than    1896 bp
  90% of sequence in fragments shorter than    2223 bp
  95% of sequence in fragments shorter than    2778 bp

# AvaI.CviQI (CYCGRG+GTAC) double digest, 6120 thousand fragments (1005714+5115078 sites), 486 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     260 bp
  15% of sequence in fragments shorter than     337 bp
  20% of sequence in fragments shorter than     408 bp
  25% of sequence in fragments shorter than     477 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     617 bp
  40% of sequence in fragments shorter than     688 bp
  45% of sequence in fragments shorter than     763 bp
  50% of sequence in fragments shorter than     843 bp
  55% of sequence in fragments shorter than     929 bp
  60% of sequence in fragments shorter than    1023 bp
  65% of sequence in fragments shorter than    1125 bp
  70% of sequence in fragments shorter than    1237 bp
  75% of sequence in fragments shorter than    1361 bp
  80% of sequence in fragments shorter than    1519 bp
  85% of sequence in fragments shorter than    1724 bp
  90% of sequence in fragments shorter than    2015 bp
  95% of sequence in fragments shorter than    2524 bp

# BsrI.SapI (ACTGG+GCTCTTC) double digest, 6117 thousand fragments (5741310+377161 sites), 487 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     250 bp
  15% of sequence in fragments shorter than     328 bp
  20% of sequence in fragments shorter than     402 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     544 bp
  35% of sequence in fragments shorter than     619 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     771 bp
  50% of sequence in fragments shorter than     857 bp
  55% of sequence in fragments shorter than     947 bp
  60% of sequence in fragments shorter than    1045 bp
  65% of sequence in fragments shorter than    1158 bp
  70% of sequence in fragments shorter than    1285 bp
  75% of sequence in fragments shorter than    1431 bp
  80% of sequence in fragments shorter than    1612 bp
  85% of sequence in fragments shorter than    1842 bp
  90% of sequence in fragments shorter than    2165 bp
  95% of sequence in fragments shorter than    2752 bp

# BsrI.SmaI (ACTGG+CCCGGG) double digest, 6117 thousand fragments (5741310+376940 sites), 487 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     251 bp
  15% of sequence in fragments shorter than     327 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     471 bp
  30% of sequence in fragments shorter than     543 bp
  35% of sequence in fragments shorter than     617 bp
  40% of sequence in fragments shorter than     691 bp
  45% of sequence in fragments shorter than     763 bp
  50% of sequence in fragments shorter than     847 bp
  55% of sequence in fragments shorter than     936 bp
  60% of sequence in fragments shorter than    1035 bp
  65% of sequence in fragments shorter than    1145 bp
  70% of sequence in fragments shorter than    1271 bp
  75% of sequence in fragments shorter than    1415 bp
  80% of sequence in fragments shorter than    1593 bp
  85% of sequence in fragments shorter than    1819 bp
  90% of sequence in fragments shorter than    2137 bp
  95% of sequence in fragments shorter than    2728 bp

# BamHI.BsrI (GGATCC+ACTGG) double digest, 6106 thousand fragments (365999+5741310 sites), 487 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     407 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     552 bp
  35% of sequence in fragments shorter than     628 bp
  40% of sequence in fragments shorter than     702 bp
  45% of sequence in fragments shorter than     778 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     954 bp
  60% of sequence in fragments shorter than    1054 bp
  65% of sequence in fragments shorter than    1168 bp
  70% of sequence in fragments shorter than    1295 bp
  75% of sequence in fragments shorter than    1443 bp
  80% of sequence in fragments shorter than    1623 bp
  85% of sequence in fragments shorter than    1856 bp
  90% of sequence in fragments shorter than    2181 bp
  95% of sequence in fragments shorter than    2765 bp

# FokI.NdeI (GGATG+CATATG) double digest, 6103 thousand fragments (5201116+903360 sites), 488 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     257 bp
  15% of sequence in fragments shorter than     335 bp
  20% of sequence in fragments shorter than     410 bp
  25% of sequence in fragments shorter than     484 bp
  30% of sequence in fragments shorter than     559 bp
  35% of sequence in fragments shorter than     631 bp
  40% of sequence in fragments shorter than     710 bp
  45% of sequence in fragments shorter than     792 bp
  50% of sequence in fragments shorter than     878 bp
  55% of sequence in fragments shorter than     972 bp
  60% of sequence in fragments shorter than    1073 bp
  65% of sequence in fragments shorter than    1182 bp
  70% of sequence in fragments shorter than    1308 bp
  75% of sequence in fragments shorter than    1445 bp
  80% of sequence in fragments shorter than    1622 bp
  85% of sequence in fragments shorter than    1846 bp
  90% of sequence in fragments shorter than    2168 bp
  95% of sequence in fragments shorter than    2740 bp

# ClaI.HphI (ATCGAT+GGTGA) double digest, 6093 thousand fragments (86477+6007336 sites), 489 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     330 bp
  20% of sequence in fragments shorter than     400 bp
  25% of sequence in fragments shorter than     472 bp
  30% of sequence in fragments shorter than     543 bp
  35% of sequence in fragments shorter than     615 bp
  40% of sequence in fragments shorter than     690 bp
  45% of sequence in fragments shorter than     769 bp
  50% of sequence in fragments shorter than     853 bp
  55% of sequence in fragments shorter than     945 bp
  60% of sequence in fragments shorter than    1046 bp
  65% of sequence in fragments shorter than    1160 bp
  70% of sequence in fragments shorter than    1288 bp
  75% of sequence in fragments shorter than    1424 bp
  80% of sequence in fragments shorter than    1605 bp
  85% of sequence in fragments shorter than    1842 bp
  90% of sequence in fragments shorter than    2165 bp
  95% of sequence in fragments shorter than    2743 bp

# BspHI.CviQI (TCATGA+GTAC) double digest, 6092 thousand fragments (978290+5115078 sites), 489 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     336 bp
  20% of sequence in fragments shorter than     409 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     548 bp
  35% of sequence in fragments shorter than     621 bp
  40% of sequence in fragments shorter than     692 bp
  45% of sequence in fragments shorter than     769 bp
  50% of sequence in fragments shorter than     850 bp
  55% of sequence in fragments shorter than     938 bp
  60% of sequence in fragments shorter than    1033 bp
  65% of sequence in fragments shorter than    1139 bp
  70% of sequence in fragments shorter than    1259 bp
  75% of sequence in fragments shorter than    1387 bp
  80% of sequence in fragments shorter than    1556 bp
  85% of sequence in fragments shorter than    1772 bp
  90% of sequence in fragments shorter than    2081 bp
  95% of sequence in fragments shorter than    2624 bp

# AccI.TfiI (GTMKAC+GAWTC) double digest, 6084 thousand fragments (1101063+4984362 sites), 489 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     258 bp
  15% of sequence in fragments shorter than     330 bp
  20% of sequence in fragments shorter than     388 bp
  25% of sequence in fragments shorter than     462 bp
  30% of sequence in fragments shorter than     528 bp
  35% of sequence in fragments shorter than     603 bp
  40% of sequence in fragments shorter than     676 bp
  45% of sequence in fragments shorter than     751 bp
  50% of sequence in fragments shorter than     829 bp
  55% of sequence in fragments shorter than     915 bp
  60% of sequence in fragments shorter than    1011 bp
  65% of sequence in fragments shorter than    1114 bp
  70% of sequence in fragments shorter than    1233 bp
  75% of sequence in fragments shorter than    1369 bp
  80% of sequence in fragments shorter than    1532 bp
  85% of sequence in fragments shorter than    1739 bp
  90% of sequence in fragments shorter than    2022 bp
  95% of sequence in fragments shorter than    2507 bp

# AatII.HphI (GACGTC+GGTGA) double digest, 6077 thousand fragments (71089+6007336 sites), 490 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     402 bp
  25% of sequence in fragments shorter than     475 bp
  30% of sequence in fragments shorter than     546 bp
  35% of sequence in fragments shorter than     618 bp
  40% of sequence in fragments shorter than     693 bp
  45% of sequence in fragments shorter than     774 bp
  50% of sequence in fragments shorter than     858 bp
  55% of sequence in fragments shorter than     951 bp
  60% of sequence in fragments shorter than    1053 bp
  65% of sequence in fragments shorter than    1168 bp
  70% of sequence in fragments shorter than    1298 bp
  75% of sequence in fragments shorter than    1435 bp
  80% of sequence in fragments shorter than    1618 bp
  85% of sequence in fragments shorter than    1859 bp
  90% of sequence in fragments shorter than    2184 bp
  95% of sequence in fragments shorter than    2769 bp

# BsrI.BstEII (ACTGG+GGTNACC) double digest, 6076 thousand fragments (5741310+335865 sites), 490 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     478 bp
  30% of sequence in fragments shorter than     551 bp
  35% of sequence in fragments shorter than     627 bp
  40% of sequence in fragments shorter than     702 bp
  45% of sequence in fragments shorter than     778 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     956 bp
  60% of sequence in fragments shorter than    1055 bp
  65% of sequence in fragments shorter than    1169 bp
  70% of sequence in fragments shorter than    1297 bp
  75% of sequence in fragments shorter than    1444 bp
  80% of sequence in fragments shorter than    1625 bp
  85% of sequence in fragments shorter than    1858 bp
  90% of sequence in fragments shorter than    2182 bp
  95% of sequence in fragments shorter than    2769 bp

# HphI.SacII (GGTGA+CCGCGG) double digest, 6073 thousand fragments (6007336+66901 sites), 490 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     402 bp
  25% of sequence in fragments shorter than     475 bp
  30% of sequence in fragments shorter than     547 bp
  35% of sequence in fragments shorter than     620 bp
  40% of sequence in fragments shorter than     695 bp
  45% of sequence in fragments shorter than     776 bp
  50% of sequence in fragments shorter than     860 bp
  55% of sequence in fragments shorter than     954 bp
  60% of sequence in fragments shorter than    1056 bp
  65% of sequence in fragments shorter than    1172 bp
  70% of sequence in fragments shorter than    1303 bp
  75% of sequence in fragments shorter than    1441 bp
  80% of sequence in fragments shorter than    1626 bp
  85% of sequence in fragments shorter than    1868 bp
  90% of sequence in fragments shorter than    2194 bp
  95% of sequence in fragments shorter than    2785 bp

# PpuMI.TfiI (RGGWCCY+GAWTC) double digest, 6068 thousand fragments (1085138+4984362 sites), 491 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     333 bp
  20% of sequence in fragments shorter than     390 bp
  25% of sequence in fragments shorter than     465 bp
  30% of sequence in fragments shorter than     537 bp
  35% of sequence in fragments shorter than     612 bp
  40% of sequence in fragments shorter than     686 bp
  45% of sequence in fragments shorter than     760 bp
  50% of sequence in fragments shorter than     839 bp
  55% of sequence in fragments shorter than     928 bp
  60% of sequence in fragments shorter than    1023 bp
  65% of sequence in fragments shorter than    1127 bp
  70% of sequence in fragments shorter than    1246 bp
  75% of sequence in fragments shorter than    1383 bp
  80% of sequence in fragments shorter than    1547 bp
  85% of sequence in fragments shorter than    1755 bp
  90% of sequence in fragments shorter than    2039 bp
  95% of sequence in fragments shorter than    2516 bp

# PvuII.TfiI (CAGCTG+GAWTC) double digest, 6068 thousand fragments (1084260+4984362 sites), 491 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     259 bp
  15% of sequence in fragments shorter than     332 bp
  20% of sequence in fragments shorter than     391 bp
  25% of sequence in fragments shorter than     466 bp
  30% of sequence in fragments shorter than     538 bp
  35% of sequence in fragments shorter than     613 bp
  40% of sequence in fragments shorter than     686 bp
  45% of sequence in fragments shorter than     760 bp
  50% of sequence in fragments shorter than     838 bp
  55% of sequence in fragments shorter than     927 bp
  60% of sequence in fragments shorter than    1022 bp
  65% of sequence in fragments shorter than    1125 bp
  70% of sequence in fragments shorter than    1243 bp
  75% of sequence in fragments shorter than    1378 bp
  80% of sequence in fragments shorter than    1541 bp
  85% of sequence in fragments shorter than    1748 bp
  90% of sequence in fragments shorter than    2029 bp
  95% of sequence in fragments shorter than    2503 bp

# HphI.PspXI (GGTGA+VCTCGAGB) double digest, 6066 thousand fragments (6007336+59193 sites), 491 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     253 bp
  15% of sequence in fragments shorter than     331 bp
  20% of sequence in fragments shorter than     402 bp
  25% of sequence in fragments shorter than     475 bp
  30% of sequence in fragments shorter than     547 bp
  35% of sequence in fragments shorter than     619 bp
  40% of sequence in fragments shorter than     694 bp
  45% of sequence in fragments shorter than     776 bp
  50% of sequence in fragments shorter than     859 bp
  55% of sequence in fragments shorter than     952 bp
  60% of sequence in fragments shorter than    1055 bp
  65% of sequence in fragments shorter than    1170 bp
  70% of sequence in fragments shorter than    1300 bp
  75% of sequence in fragments shorter than    1438 bp
  80% of sequence in fragments shorter than    1622 bp
  85% of sequence in fragments shorter than    1863 bp
  90% of sequence in fragments shorter than    2189 bp
  95% of sequence in fragments shorter than    2776 bp

# BbvI.BglII (GCAGC+AGATCT) double digest, 6064 thousand fragments (5290051+774732 sites), 491 bp average fragment length
   5% of sequence in fragments shorter than     164 bp
  10% of sequence in fragments shorter than     262 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     439 bp
  25% of sequence in fragments shorter than     528 bp
  30% of sequence in fragments shorter than     617 bp
  35% of sequence in fragments shorter than     709 bp
  40% of sequence in fragments shorter than     805 bp
  45% of sequence in fragments shorter than     902 bp
  50% of sequence in fragments shorter than    1012 bp
  55% of sequence in fragments shorter than    1130 bp
  60% of sequence in fragments shorter than    1258 bp
  65% of sequence in fragments shorter than    1399 bp
  70% of sequence in fragments shorter than    1559 bp
  75% of sequence in fragments shorter than    1739 bp
  80% of sequence in fragments shorter than    1970 bp
  85% of sequence in fragments shorter than    2266 bp
  90% of sequence in fragments shorter than    2685 bp
  95% of sequence in fragments shorter than    3400 bp

# FokI.HindIII (GGATG+AAGCTT) double digest, 6060 thousand fragments (5201116+860365 sites), 491 bp average fragment length
   5% of sequence in fragments shorter than     170 bp
  10% of sequence in fragments shorter than     262 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     412 bp
  25% of sequence in fragments shorter than     487 bp
  30% of sequence in fragments shorter than     562 bp
  35% of sequence in fragments shorter than     639 bp
  40% of sequence in fragments shorter than     718 bp
  45% of sequence in fragments shorter than     800 bp
  50% of sequence in fragments shorter than     887 bp
  55% of sequence in fragments shorter than     976 bp
  60% of sequence in fragments shorter than    1075 bp
  65% of sequence in fragments shorter than    1186 bp
  70% of sequence in fragments shorter than    1313 bp
  75% of sequence in fragments shorter than    1450 bp
  80% of sequence in fragments shorter than    1627 bp
  85% of sequence in fragments shorter than    1846 bp
  90% of sequence in fragments shorter than    2163 bp
  95% of sequence in fragments shorter than    2723 bp

# AgeI.HphI (ACCGGT+GGTGA) double digest, 6060 thousand fragments (53386+6007336 sites), 491 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     254 bp
  15% of sequence in fragments shorter than     332 bp
  20% of sequence in fragments shorter than     403 bp
  25% of sequence in fragments shorter than     477 bp
  30% of sequence in fragments shorter than     548 bp
  35% of sequence in fragments shorter than     621 bp
  40% of sequence in fragments shorter than     696 bp
  45% of sequence in fragments shorter than     777 bp
  50% of sequence in fragments shorter than     861 bp
  55% of sequence in fragments shorter than     954 bp
  60% of sequence in fragments shorter than    1057 bp
  65% of sequence in fragments shorter than    1172 bp
  70% of sequence in fragments shorter than    1302 bp
  75% of sequence in fragments shorter than    1439 bp
  80% of sequence in fragments shorter than    1623 bp
  85% of sequence in fragments shorter than    1865 bp
  90% of sequence in fragments shorter than    2189 bp
  95% of sequence in fragments shorter than    2777 bp

# FokI.XbaI (GGATG+TCTAGA) double digest, 6051 thousand fragments (5201116+850998 sites), 492 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     261 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     415 bp
  25% of sequence in fragments shorter than     489 bp
  30% of sequence in fragments shorter than     566 bp
  35% of sequence in fragments shorter than     643 bp
  40% of sequence in fragments shorter than     722 bp
  45% of sequence in fragments shorter than     805 bp
  50% of sequence in fragments shorter than     893 bp
  55% of sequence in fragments shorter than     983 bp
  60% of sequence in fragments shorter than    1083 bp
  65% of sequence in fragments shorter than    1188 bp
  70% of sequence in fragments shorter than    1316 bp
  75% of sequence in fragments shorter than    1459 bp
  80% of sequence in fragments shorter than    1638 bp
  85% of sequence in fragments shorter than    1859 bp
  90% of sequence in fragments shorter than    2177 bp
  95% of sequence in fragments shorter than    2723 bp

# BbvI.NcoI (GCAGC+CCATGG) double digest, 6049 thousand fragments (5290051+759594 sites), 492 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     353 bp
  20% of sequence in fragments shorter than     446 bp
  25% of sequence in fragments shorter than     542 bp
  30% of sequence in fragments shorter than     640 bp
  35% of sequence in fragments shorter than     741 bp
  40% of sequence in fragments shorter than     846 bp
  45% of sequence in fragments shorter than     955 bp
  50% of sequence in fragments shorter than    1074 bp
  55% of sequence in fragments shorter than    1206 bp
  60% of sequence in fragments shorter than    1348 bp
  65% of sequence in fragments shorter than    1509 bp
  70% of sequence in fragments shorter than    1681 bp
  75% of sequence in fragments shorter than    1895 bp
  80% of sequence in fragments shorter than    2138 bp
  85% of sequence in fragments shorter than    2475 bp
  90% of sequence in fragments shorter than    2935 bp
  95% of sequence in fragments shorter than    3744 bp

# HphI.PmeI (GGTGA+GTTTAAAC) double digest, 6048 thousand fragments (6007336+42094 sites), 492 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     254 bp
  15% of sequence in fragments shorter than     332 bp
  20% of sequence in fragments shorter than     403 bp
  25% of sequence in fragments shorter than     476 bp
  30% of sequence in fragments shorter than     548 bp
  35% of sequence in fragments shorter than     620 bp
  40% of sequence in fragments shorter than     695 bp
  45% of sequence in fragments shorter than     776 bp
  50% of sequence in fragments shorter than     859 bp
  55% of sequence in fragments shorter than     952 bp
  60% of sequence in fragments shorter than    1054 bp
  65% of sequence in fragments shorter than    1169 bp
  70% of sequence in fragments shorter than    1299 bp
  75% of sequence in fragments shorter than    1435 bp
  80% of sequence in fragments shorter than    1618 bp
  85% of sequence in fragments shorter than    1858 bp
  90% of sequence in fragments shorter than    2181 bp
  95% of sequence in fragments shorter than    2763 bp

# EcoRI.FokI (GAATTC+GGATG) double digest, 6048 thousand fragments (847401+5201116 sites), 492 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     411 bp
  25% of sequence in fragments shorter than     488 bp
  30% of sequence in fragments shorter than     563 bp
  35% of sequence in fragments shorter than     639 bp
  40% of sequence in fragments shorter than     715 bp
  45% of sequence in fragments shorter than     797 bp
  50% of sequence in fragments shorter than     885 bp
  55% of sequence in fragments shorter than     975 bp
  60% of sequence in fragments shorter than    1074 bp
  65% of sequence in fragments shorter than    1182 bp
  70% of sequence in fragments shorter than    1303 bp
  75% of sequence in fragments shorter than    1441 bp
  80% of sequence in fragments shorter than    1617 bp
  85% of sequence in fragments shorter than    1839 bp
  90% of sequence in fragments shorter than    2150 bp
  95% of sequence in fragments shorter than    2680 bp

# AvaIII.CviQI (ATGCAT+GTAC) double digest, 6047 thousand fragments (933357+5115078 sites), 492 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     262 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     418 bp
  25% of sequence in fragments shorter than     489 bp
  30% of sequence in fragments shorter than     559 bp
  35% of sequence in fragments shorter than     635 bp
  40% of sequence in fragments shorter than     709 bp
  45% of sequence in fragments shorter than     788 bp
  50% of sequence in fragments shorter than     874 bp
  55% of sequence in fragments shorter than     964 bp
  60% of sequence in fragments shorter than    1061 bp
  65% of sequence in fragments shorter than    1173 bp
  70% of sequence in fragments shorter than    1293 bp
  75% of sequence in fragments shorter than    1424 bp
  80% of sequence in fragments shorter than    1599 bp
  85% of sequence in fragments shorter than    1826 bp
  90% of sequence in fragments shorter than    2139 bp
  95% of sequence in fragments shorter than    2706 bp

# CviQI.StuI (GTAC+AGGCCT) double digest, 6040 thousand fragments (5115078+925729 sites), 493 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     410 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     550 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     690 bp
  45% of sequence in fragments shorter than     768 bp
  50% of sequence in fragments shorter than     851 bp
  55% of sequence in fragments shorter than     934 bp
  60% of sequence in fragments shorter than    1029 bp
  65% of sequence in fragments shorter than    1135 bp
  70% of sequence in fragments shorter than    1252 bp
  75% of sequence in fragments shorter than    1377 bp
  80% of sequence in fragments shorter than    1539 bp
  85% of sequence in fragments shorter than    1741 bp
  90% of sequence in fragments shorter than    2024 bp
  95% of sequence in fragments shorter than    2494 bp

# BsrI.SexAI (ACTGG+ACCWGGT) double digest, 6038 thousand fragments (5741310+297900 sites), 493 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     254 bp
  15% of sequence in fragments shorter than     332 bp
  20% of sequence in fragments shorter than     408 bp
  25% of sequence in fragments shorter than     481 bp
  30% of sequence in fragments shorter than     555 bp
  35% of sequence in fragments shorter than     631 bp
  40% of sequence in fragments shorter than     705 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     866 bp
  55% of sequence in fragments shorter than     958 bp
  60% of sequence in fragments shorter than    1058 bp
  65% of sequence in fragments shorter than    1171 bp
  70% of sequence in fragments shorter than    1300 bp
  75% of sequence in fragments shorter than    1446 bp
  80% of sequence in fragments shorter than    1627 bp
  85% of sequence in fragments shorter than    1860 bp
  90% of sequence in fragments shorter than    2182 bp
  95% of sequence in fragments shorter than    2760 bp

# HphI.SalI (GGTGA+GTCGAC) double digest, 6038 thousand fragments (6007336+31396 sites), 493 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     255 bp
  15% of sequence in fragments shorter than     333 bp
  20% of sequence in fragments shorter than     404 bp
  25% of sequence in fragments shorter than     477 bp
  30% of sequence in fragments shorter than     549 bp
  35% of sequence in fragments shorter than     622 bp
  40% of sequence in fragments shorter than     697 bp
  45% of sequence in fragments shorter than     779 bp
  50% of sequence in fragments shorter than     862 bp
  55% of sequence in fragments shorter than     956 bp
  60% of sequence in fragments shorter than    1058 bp
  65% of sequence in fragments shorter than    1174 bp
  70% of sequence in fragments shorter than    1304 bp
  75% of sequence in fragments shorter than    1442 bp
  80% of sequence in fragments shorter than    1626 bp
  85% of sequence in fragments shorter than    1868 bp
  90% of sequence in fragments shorter than    2193 bp
  95% of sequence in fragments shorter than    2782 bp

# BscAI.SspI (GCATC+AATATT) double digest, 6029 thousand fragments (3652926+2377267 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     170 bp
  10% of sequence in fragments shorter than     257 bp
  15% of sequence in fragments shorter than     336 bp
  20% of sequence in fragments shorter than     411 bp
  25% of sequence in fragments shorter than     483 bp
  30% of sequence in fragments shorter than     556 bp
  35% of sequence in fragments shorter than     629 bp
  40% of sequence in fragments shorter than     703 bp
  45% of sequence in fragments shorter than     784 bp
  50% of sequence in fragments shorter than     868 bp
  55% of sequence in fragments shorter than     961 bp
  60% of sequence in fragments shorter than    1062 bp
  65% of sequence in fragments shorter than    1170 bp
  70% of sequence in fragments shorter than    1295 bp
  75% of sequence in fragments shorter than    1440 bp
  80% of sequence in fragments shorter than    1607 bp
  85% of sequence in fragments shorter than    1831 bp
  90% of sequence in fragments shorter than    2146 bp
  95% of sequence in fragments shorter than    2729 bp

# BsrI.KpnI (ACTGG+GGTACC) double digest, 6028 thousand fragments (5741310+287974 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     169 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     336 bp
  20% of sequence in fragments shorter than     413 bp
  25% of sequence in fragments shorter than     485 bp
  30% of sequence in fragments shorter than     560 bp
  35% of sequence in fragments shorter than     637 bp
  40% of sequence in fragments shorter than     708 bp
  45% of sequence in fragments shorter than     786 bp
  50% of sequence in fragments shorter than     873 bp
  55% of sequence in fragments shorter than     967 bp
  60% of sequence in fragments shorter than    1068 bp
  65% of sequence in fragments shorter than    1183 bp
  70% of sequence in fragments shorter than    1313 bp
  75% of sequence in fragments shorter than    1460 bp
  80% of sequence in fragments shorter than    1643 bp
  85% of sequence in fragments shorter than    1879 bp
  90% of sequence in fragments shorter than    2207 bp
  95% of sequence in fragments shorter than    2798 bp

# BbvI.BclI (GCAGC+TGATCA) double digest, 6028 thousand fragments (5290051+738785 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     351 bp
  20% of sequence in fragments shorter than     442 bp
  25% of sequence in fragments shorter than     532 bp
  30% of sequence in fragments shorter than     622 bp
  35% of sequence in fragments shorter than     715 bp
  40% of sequence in fragments shorter than     816 bp
  45% of sequence in fragments shorter than     917 bp
  50% of sequence in fragments shorter than    1028 bp
  55% of sequence in fragments shorter than    1147 bp
  60% of sequence in fragments shorter than    1275 bp
  65% of sequence in fragments shorter than    1421 bp
  70% of sequence in fragments shorter than    1588 bp
  75% of sequence in fragments shorter than    1767 bp
  80% of sequence in fragments shorter than    1998 bp
  85% of sequence in fragments shorter than    2281 bp
  90% of sequence in fragments shorter than    2704 bp
  95% of sequence in fragments shorter than    3419 bp

# HphI.MluI (GGTGA+ACGCGT) double digest, 6028 thousand fragments (6007336+21415 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     255 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     405 bp
  25% of sequence in fragments shorter than     478 bp
  30% of sequence in fragments shorter than     550 bp
  35% of sequence in fragments shorter than     623 bp
  40% of sequence in fragments shorter than     698 bp
  45% of sequence in fragments shorter than     780 bp
  50% of sequence in fragments shorter than     863 bp
  55% of sequence in fragments shorter than     958 bp
  60% of sequence in fragments shorter than    1061 bp
  65% of sequence in fragments shorter than    1177 bp
  70% of sequence in fragments shorter than    1307 bp
  75% of sequence in fragments shorter than    1445 bp
  80% of sequence in fragments shorter than    1630 bp
  85% of sequence in fragments shorter than    1872 bp
  90% of sequence in fragments shorter than    2198 bp
  95% of sequence in fragments shorter than    2788 bp

# BsrI.NheI (ACTGG+GCTAGC) double digest, 6028 thousand fragments (5741310+287384 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     255 bp
  15% of sequence in fragments shorter than     335 bp
  20% of sequence in fragments shorter than     411 bp
  25% of sequence in fragments shorter than     483 bp
  30% of sequence in fragments shorter than     557 bp
  35% of sequence in fragments shorter than     634 bp
  40% of sequence in fragments shorter than     708 bp
  45% of sequence in fragments shorter than     785 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     964 bp
  60% of sequence in fragments shorter than    1065 bp
  65% of sequence in fragments shorter than    1179 bp
  70% of sequence in fragments shorter than    1308 bp
  75% of sequence in fragments shorter than    1455 bp
  80% of sequence in fragments shorter than    1636 bp
  85% of sequence in fragments shorter than    1868 bp
  90% of sequence in fragments shorter than    2195 bp
  95% of sequence in fragments shorter than    2776 bp

# BbvI.FnuDII (GCAGC+CGCG) double digest, 6023 thousand fragments (5290051+733940 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     164 bp
  10% of sequence in fragments shorter than     269 bp
  15% of sequence in fragments shorter than     365 bp
  20% of sequence in fragments shorter than     464 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     667 bp
  35% of sequence in fragments shorter than     773 bp
  40% of sequence in fragments shorter than     878 bp
  45% of sequence in fragments shorter than     996 bp
  50% of sequence in fragments shorter than    1122 bp
  55% of sequence in fragments shorter than    1259 bp
  60% of sequence in fragments shorter than    1409 bp
  65% of sequence in fragments shorter than    1580 bp
  70% of sequence in fragments shorter than    1762 bp
  75% of sequence in fragments shorter than    1983 bp
  80% of sequence in fragments shorter than    2247 bp
  85% of sequence in fragments shorter than    2603 bp
  90% of sequence in fragments shorter than    3094 bp
  95% of sequence in fragments shorter than    3955 bp

# HphI.SgrAI (GGTGA+CRCCGGYG) double digest, 6022 thousand fragments (6007336+15270 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     551 bp
  35% of sequence in fragments shorter than     624 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     959 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1179 bp
  70% of sequence in fragments shorter than    1309 bp
  75% of sequence in fragments shorter than    1447 bp
  80% of sequence in fragments shorter than    1632 bp
  85% of sequence in fragments shorter than    1875 bp
  90% of sequence in fragments shorter than    2201 bp
  95% of sequence in fragments shorter than    2792 bp

# HphI.NruI (GGTGA+TCGCGA) double digest, 6021 thousand fragments (6007336+14872 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     405 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     551 bp
  35% of sequence in fragments shorter than     624 bp
  40% of sequence in fragments shorter than     699 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     863 bp
  55% of sequence in fragments shorter than     959 bp
  60% of sequence in fragments shorter than    1062 bp
  65% of sequence in fragments shorter than    1178 bp
  70% of sequence in fragments shorter than    1308 bp
  75% of sequence in fragments shorter than    1447 bp
  80% of sequence in fragments shorter than    1632 bp
  85% of sequence in fragments shorter than    1874 bp
  90% of sequence in fragments shorter than    2200 bp
  95% of sequence in fragments shorter than    2790 bp

# HphI.PvuI (GGTGA+CGATCG) double digest, 6021 thousand fragments (6007336+14186 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     551 bp
  35% of sequence in fragments shorter than     624 bp
  40% of sequence in fragments shorter than     699 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     959 bp
  60% of sequence in fragments shorter than    1062 bp
  65% of sequence in fragments shorter than    1178 bp
  70% of sequence in fragments shorter than    1308 bp
  75% of sequence in fragments shorter than    1447 bp
  80% of sequence in fragments shorter than    1632 bp
  85% of sequence in fragments shorter than    1874 bp
  90% of sequence in fragments shorter than    2200 bp
  95% of sequence in fragments shorter than    2790 bp

# FseI.HphI (GGCCGGCC+GGTGA) double digest, 6020 thousand fragments (13662+6007336 sites), 494 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     405 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     551 bp
  35% of sequence in fragments shorter than     624 bp
  40% of sequence in fragments shorter than     699 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     959 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1179 bp
  70% of sequence in fragments shorter than    1309 bp
  75% of sequence in fragments shorter than    1447 bp
  80% of sequence in fragments shorter than    1632 bp
  85% of sequence in fragments shorter than    1875 bp
  90% of sequence in fragments shorter than    2201 bp
  95% of sequence in fragments shorter than    2793 bp

# CviQI.NdeI (GTAC+CATATG) double digest, 6017 thousand fragments (5115078+903360 sites), 495 bp average fragment length
   5% of sequence in fragments shorter than     169 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     419 bp
  25% of sequence in fragments shorter than     493 bp
  30% of sequence in fragments shorter than     566 bp
  35% of sequence in fragments shorter than     642 bp
  40% of sequence in fragments shorter than     717 bp
  45% of sequence in fragments shorter than     797 bp
  50% of sequence in fragments shorter than     882 bp
  55% of sequence in fragments shorter than     973 bp
  60% of sequence in fragments shorter than    1073 bp
  65% of sequence in fragments shorter than    1179 bp
  70% of sequence in fragments shorter than    1303 bp
  75% of sequence in fragments shorter than    1449 bp
  80% of sequence in fragments shorter than    1628 bp
  85% of sequence in fragments shorter than    1863 bp
  90% of sequence in fragments shorter than    2192 bp
  95% of sequence in fragments shorter than    2801 bp

# HphI.NotI (GGTGA+GCGGCCGC) double digest, 6016 thousand fragments (6007336+9795 sites), 495 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     551 bp
  35% of sequence in fragments shorter than     624 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     959 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1179 bp
  70% of sequence in fragments shorter than    1309 bp
  75% of sequence in fragments shorter than    1448 bp
  80% of sequence in fragments shorter than    1633 bp
  85% of sequence in fragments shorter than    1875 bp
  90% of sequence in fragments shorter than    2201 bp
  95% of sequence in fragments shorter than    2792 bp

# HphI.RsrII (GGTGA+CGGWCCG) double digest, 6016 thousand fragments (6007336+9764 sites), 495 bp average fragment length
   5% of sequence in fragments shorter than     163 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     334 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     479 bp
  30% of sequence in fragments shorter than     552 bp
  35% of sequence in fragments shorter than     624 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     960 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1179 bp
  70% of sequence in fragments shorter than    1309 bp
  75% of sequence in fragments shorter than    1448 bp
  80% of sequence in fragments shorter than    1633 bp
  85% of sequence in fragments shorter than    1875 bp
  90% of sequence in fragments shorter than    2201 bp
  95% of sequence in fragments shorter than    2792 bp

# AscI.HphI (GGCGCGCC+GGTGA) double digest, 6011 thousand fragments (4751+6007336 sites), 495 bp average fragment length
   5% of sequence in fragments shorter than     164 bp
  10% of sequence in fragments shorter than     256 bp
  15% of sequence in fragments shorter than     335 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     480 bp
  30% of sequence in fragments shorter than     552 bp
  35% of sequence in fragments shorter than     625 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     782 bp
  50% of sequence in fragments shorter than     864 bp
  55% of sequence in fragments shorter than     960 bp
  60% of sequence in fragments shorter than    1063 bp
  65% of sequence in fragments shorter than    1180 bp
  70% of sequence in fragments shorter than    1310 bp
  75% of sequence in fragments shorter than    1449 bp
  80% of sequence in fragments shorter than    1634 bp
  85% of sequence in fragments shorter than    1876 bp
  90% of sequence in fragments shorter than    2203 bp
  95% of sequence in fragments shorter than    2794 bp

# BbvI.BsaAI (GCAGC+YACGTR) double digest, 6003 thousand fragments (5290051+713801 sites), 496 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     354 bp
  20% of sequence in fragments shorter than     446 bp
  25% of sequence in fragments shorter than     539 bp
  30% of sequence in fragments shorter than     634 bp
  35% of sequence in fragments shorter than     732 bp
  40% of sequence in fragments shorter than     834 bp
  45% of sequence in fragments shorter than     939 bp
  50% of sequence in fragments shorter than    1051 bp
  55% of sequence in fragments shorter than    1179 bp
  60% of sequence in fragments shorter than    1317 bp
  65% of sequence in fragments shorter than    1470 bp
  70% of sequence in fragments shorter than    1630 bp
  75% of sequence in fragments shorter than    1831 bp
  80% of sequence in fragments shorter than    2071 bp
  85% of sequence in fragments shorter than    2385 bp
  90% of sequence in fragments shorter than    2821 bp
  95% of sequence in fragments shorter than    3580 bp

# BbvI.BceAI (GCAGC+ACGGC) double digest, 5994 thousand fragments (5290051+705178 sites), 497 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     358 bp
  20% of sequence in fragments shorter than     456 bp
  25% of sequence in fragments shorter than     555 bp
  30% of sequence in fragments shorter than     655 bp
  35% of sequence in fragments shorter than     759 bp
  40% of sequence in fragments shorter than     863 bp
  45% of sequence in fragments shorter than     978 bp
  50% of sequence in fragments shorter than    1101 bp
  55% of sequence in fragments shorter than    1235 bp
  60% of sequence in fragments shorter than    1382 bp
  65% of sequence in fragments shorter than    1550 bp
  70% of sequence in fragments shorter than    1728 bp
  75% of sequence in fragments shorter than    1946 bp
  80% of sequence in fragments shorter than    2201 bp
  85% of sequence in fragments shorter than    2548 bp
  90% of sequence in fragments shorter than    3020 bp
  95% of sequence in fragments shorter than    3854 bp

# AvaI.TfiI (CYCGRG+GAWTC) double digest, 5989 thousand fragments (1005714+4984362 sites), 497 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     269 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     404 bp
  25% of sequence in fragments shorter than     480 bp
  30% of sequence in fragments shorter than     554 bp
  35% of sequence in fragments shorter than     631 bp
  40% of sequence in fragments shorter than     703 bp
  45% of sequence in fragments shorter than     784 bp
  50% of sequence in fragments shorter than     865 bp
  55% of sequence in fragments shorter than     956 bp
  60% of sequence in fragments shorter than    1053 bp
  65% of sequence in fragments shorter than    1163 bp
  70% of sequence in fragments shorter than    1286 bp
  75% of sequence in fragments shorter than    1428 bp
  80% of sequence in fragments shorter than    1598 bp
  85% of sequence in fragments shorter than    1813 bp
  90% of sequence in fragments shorter than    2106 bp
  95% of sequence in fragments shorter than    2597 bp

# BbvI.BciVI (GCAGC+GTATCC) double digest, 5985 thousand fragments (5290051+696093 sites), 497 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     353 bp
  20% of sequence in fragments shorter than     444 bp
  25% of sequence in fragments shorter than     535 bp
  30% of sequence in fragments shorter than     624 bp
  35% of sequence in fragments shorter than     719 bp
  40% of sequence in fragments shorter than     818 bp
  45% of sequence in fragments shorter than     913 bp
  50% of sequence in fragments shorter than    1025 bp
  55% of sequence in fragments shorter than    1142 bp
  60% of sequence in fragments shorter than    1270 bp
  65% of sequence in fragments shorter than    1414 bp
  70% of sequence in fragments shorter than    1580 bp
  75% of sequence in fragments shorter than    1774 bp
  80% of sequence in fragments shorter than    2013 bp
  85% of sequence in fragments shorter than    2307 bp
  90% of sequence in fragments shorter than    2733 bp
  95% of sequence in fragments shorter than    3458 bp

# BsrBI.BsrI (CCGCTC+ACTGG) double digest, 5976 thousand fragments (235523+5741310 sites), 498 bp average fragment length
   5% of sequence in fragments shorter than     169 bp
  10% of sequence in fragments shorter than     257 bp
  15% of sequence in fragments shorter than     337 bp
  20% of sequence in fragments shorter than     414 bp
  25% of sequence in fragments shorter than     487 bp
  30% of sequence in fragments shorter than     562 bp
  35% of sequence in fragments shorter than     639 bp
  40% of sequence in fragments shorter than     711 bp
  45% of sequence in fragments shorter than     792 bp
  50% of sequence in fragments shorter than     876 bp
  55% of sequence in fragments shorter than     972 bp
  60% of sequence in fragments shorter than    1074 bp
  65% of sequence in fragments shorter than    1189 bp
  70% of sequence in fragments shorter than    1321 bp
  75% of sequence in fragments shorter than    1469 bp
  80% of sequence in fragments shorter than    1653 bp
  85% of sequence in fragments shorter than    1890 bp
  90% of sequence in fragments shorter than    2222 bp
  95% of sequence in fragments shorter than    2815 bp

# BglII.FokI (AGATCT+GGATG) double digest, 5975 thousand fragments (774732+5201116 sites), 498 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     345 bp
  20% of sequence in fragments shorter than     420 bp
  25% of sequence in fragments shorter than     496 bp
  30% of sequence in fragments shorter than     571 bp
  35% of sequence in fragments shorter than     646 bp
  40% of sequence in fragments shorter than     726 bp
  45% of sequence in fragments shorter than     809 bp
  50% of sequence in fragments shorter than     898 bp
  55% of sequence in fragments shorter than     990 bp
  60% of sequence in fragments shorter than    1090 bp
  65% of sequence in fragments shorter than    1203 bp
  70% of sequence in fragments shorter than    1332 bp
  75% of sequence in fragments shorter than    1477 bp
  80% of sequence in fragments shorter than    1658 bp
  85% of sequence in fragments shorter than    1888 bp
  90% of sequence in fragments shorter than    2222 bp
  95% of sequence in fragments shorter than    2819 bp

# CviQI.HindIII (GTAC+AAGCTT) double digest, 5974 thousand fragments (5115078+860365 sites), 498 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     344 bp
  20% of sequence in fragments shorter than     417 bp
  25% of sequence in fragments shorter than     489 bp
  30% of sequence in fragments shorter than     559 bp
  35% of sequence in fragments shorter than     634 bp
  40% of sequence in fragments shorter than     708 bp
  45% of sequence in fragments shorter than     788 bp
  50% of sequence in fragments shorter than     873 bp
  55% of sequence in fragments shorter than     962 bp
  60% of sequence in fragments shorter than    1054 bp
  65% of sequence in fragments shorter than    1164 bp
  70% of sequence in fragments shorter than    1286 bp
  75% of sequence in fragments shorter than    1414 bp
  80% of sequence in fragments shorter than    1585 bp
  85% of sequence in fragments shorter than    1807 bp
  90% of sequence in fragments shorter than    2119 bp
  95% of sequence in fragments shorter than    2672 bp

# BsrI.KasI (ACTGG+GGCGCC) double digest, 5971 thousand fragments (5741310+230381 sites), 499 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     258 bp
  15% of sequence in fragments shorter than     336 bp
  20% of sequence in fragments shorter than     412 bp
  25% of sequence in fragments shorter than     485 bp
  30% of sequence in fragments shorter than     559 bp
  35% of sequence in fragments shorter than     635 bp
  40% of sequence in fragments shorter than     708 bp
  45% of sequence in fragments shorter than     785 bp
  50% of sequence in fragments shorter than     869 bp
  55% of sequence in fragments shorter than     962 bp
  60% of sequence in fragments shorter than    1062 bp
  65% of sequence in fragments shorter than    1176 bp
  70% of sequence in fragments shorter than    1305 bp
  75% of sequence in fragments shorter than    1452 bp
  80% of sequence in fragments shorter than    1633 bp
  85% of sequence in fragments shorter than    1866 bp
  90% of sequence in fragments shorter than    2190 bp
  95% of sequence in fragments shorter than    2775 bp

# BscAI.HpaII (GCATC+CCGG) double digest, 5970 thousand fragments (3652926+2317722 sites), 499 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     258 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     435 bp
  25% of sequence in fragments shorter than     520 bp
  30% of sequence in fragments shorter than     604 bp
  35% of sequence in fragments shorter than     690 bp
  40% of sequence in fragments shorter than     780 bp
  45% of sequence in fragments shorter than     875 bp
  50% of sequence in fragments shorter than     979 bp
  55% of sequence in fragments shorter than    1092 bp
  60% of sequence in fragments shorter than    1214 bp
  65% of sequence in fragments shorter than    1347 bp
  70% of sequence in fragments shorter than    1500 bp
  75% of sequence in fragments shorter than    1672 bp
  80% of sequence in fragments shorter than    1892 bp
  85% of sequence in fragments shorter than    2169 bp
  90% of sequence in fragments shorter than    2579 bp
  95% of sequence in fragments shorter than    3316 bp

# BsrI.SwaI (ACTGG+ATTTAAAT) double digest, 5967 thousand fragments (5741310+226602 sites), 499 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     258 bp
  15% of sequence in fragments shorter than     337 bp
  20% of sequence in fragments shorter than     414 bp
  25% of sequence in fragments shorter than     486 bp
  30% of sequence in fragments shorter than     560 bp
  35% of sequence in fragments shorter than     635 bp
  40% of sequence in fragments shorter than     708 bp
  45% of sequence in fragments shorter than     784 bp
  50% of sequence in fragments shorter than     868 bp
  55% of sequence in fragments shorter than     960 bp
  60% of sequence in fragments shorter than    1059 bp
  65% of sequence in fragments shorter than    1172 bp
  70% of sequence in fragments shorter than    1298 bp
  75% of sequence in fragments shorter than    1442 bp
  80% of sequence in fragments shorter than    1619 bp
  85% of sequence in fragments shorter than    1846 bp
  90% of sequence in fragments shorter than    2162 bp
  95% of sequence in fragments shorter than    2742 bp

# CviQI.XbaI (GTAC+TCTAGA) double digest, 5965 thousand fragments (5115078+850998 sites), 499 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     345 bp
  20% of sequence in fragments shorter than     421 bp
  25% of sequence in fragments shorter than     493 bp
  30% of sequence in fragments shorter than     557 bp
  35% of sequence in fragments shorter than     633 bp
  40% of sequence in fragments shorter than     705 bp
  45% of sequence in fragments shorter than     785 bp
  50% of sequence in fragments shorter than     863 bp
  55% of sequence in fragments shorter than     951 bp
  60% of sequence in fragments shorter than    1048 bp
  65% of sequence in fragments shorter than    1159 bp
  70% of sequence in fragments shorter than    1280 bp
  75% of sequence in fragments shorter than    1416 bp
  80% of sequence in fragments shorter than    1589 bp
  85% of sequence in fragments shorter than    1811 bp
  90% of sequence in fragments shorter than    2122 bp
  95% of sequence in fragments shorter than    2674 bp

# BspHI.TfiI (TCATGA+GAWTC) double digest, 5962 thousand fragments (978290+4984362 sites), 499 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     402 bp
  25% of sequence in fragments shorter than     478 bp
  30% of sequence in fragments shorter than     551 bp
  35% of sequence in fragments shorter than     625 bp
  40% of sequence in fragments shorter than     700 bp
  45% of sequence in fragments shorter than     781 bp
  50% of sequence in fragments shorter than     863 bp
  55% of sequence in fragments shorter than     955 bp
  60% of sequence in fragments shorter than    1054 bp
  65% of sequence in fragments shorter than    1164 bp
  70% of sequence in fragments shorter than    1288 bp
  75% of sequence in fragments shorter than    1431 bp
  80% of sequence in fragments shorter than    1605 bp
  85% of sequence in fragments shorter than    1822 bp
  90% of sequence in fragments shorter than    2118 bp
  95% of sequence in fragments shorter than    2625 bp

# AciI.HhaI (CCGC+GCGC) double digest, 5962 thousand fragments (4206130+1756502 sites), 499 bp average fragment length
   5% of sequence in fragments shorter than     160 bp
  10% of sequence in fragments shorter than     310 bp
  15% of sequence in fragments shorter than     452 bp
  20% of sequence in fragments shorter than     604 bp
  25% of sequence in fragments shorter than     753 bp
  30% of sequence in fragments shorter than     913 bp
  35% of sequence in fragments shorter than    1087 bp
  40% of sequence in fragments shorter than    1275 bp
  45% of sequence in fragments shorter than    1478 bp
  50% of sequence in fragments shorter than    1705 bp
  55% of sequence in fragments shorter than    1953 bp
  60% of sequence in fragments shorter than    2233 bp
  65% of sequence in fragments shorter than    2552 bp
  70% of sequence in fragments shorter than    2915 bp
  75% of sequence in fragments shorter than    3351 bp
  80% of sequence in fragments shorter than    3889 bp
  85% of sequence in fragments shorter than    4576 bp
  90% of sequence in fragments shorter than    5540 bp
  95% of sequence in fragments shorter than    7212 bp

# CviQI.EcoRI (GTAC+GAATTC) double digest, 5961 thousand fragments (5115078+847401 sites), 499 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     345 bp
  20% of sequence in fragments shorter than     419 bp
  25% of sequence in fragments shorter than     491 bp
  30% of sequence in fragments shorter than     561 bp
  35% of sequence in fragments shorter than     636 bp
  40% of sequence in fragments shorter than     708 bp
  45% of sequence in fragments shorter than     786 bp
  50% of sequence in fragments shorter than     870 bp
  55% of sequence in fragments shorter than     959 bp
  60% of sequence in fragments shorter than    1057 bp
  65% of sequence in fragments shorter than    1169 bp
  70% of sequence in fragments shorter than    1287 bp
  75% of sequence in fragments shorter than    1416 bp
  80% of sequence in fragments shorter than    1586 bp
  85% of sequence in fragments shorter than    1806 bp
  90% of sequence in fragments shorter than    2114 bp
  95% of sequence in fragments shorter than    2647 bp

# FokI.NcoI (GGATG+CCATGG) double digest, 5960 thousand fragments (5201116+759594 sites), 499 bp average fragment length
   5% of sequence in fragments shorter than     169 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     426 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     585 bp
  35% of sequence in fragments shorter than     667 bp
  40% of sequence in fragments shorter than     752 bp
  45% of sequence in fragments shorter than     839 bp
  50% of sequence in fragments shorter than     930 bp
  55% of sequence in fragments shorter than    1031 bp
  60% of sequence in fragments shorter than    1138 bp
  65% of sequence in fragments shorter than    1257 bp
  70% of sequence in fragments shorter than    1390 bp
  75% of sequence in fragments shorter than    1548 bp
  80% of sequence in fragments shorter than    1739 bp
  85% of sequence in fragments shorter than    1990 bp
  90% of sequence in fragments shorter than    2348 bp
  95% of sequence in fragments shorter than    2985 bp

# BclI.FokI (TGATCA+GGATG) double digest, 5939 thousand fragments (738785+5201116 sites), 501 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     424 bp
  25% of sequence in fragments shorter than     501 bp
  30% of sequence in fragments shorter than     578 bp
  35% of sequence in fragments shorter than     657 bp
  40% of sequence in fragments shorter than     738 bp
  45% of sequence in fragments shorter than     823 bp
  50% of sequence in fragments shorter than     911 bp
  55% of sequence in fragments shorter than    1003 bp
  60% of sequence in fragments shorter than    1104 bp
  65% of sequence in fragments shorter than    1215 bp
  70% of sequence in fragments shorter than    1346 bp
  75% of sequence in fragments shorter than    1490 bp
  80% of sequence in fragments shorter than    1671 bp
  85% of sequence in fragments shorter than    1900 bp
  90% of sequence in fragments shorter than    2235 bp
  95% of sequence in fragments shorter than    2819 bp

# FnuDII.FokI (CGCG+GGATG) double digest, 5934 thousand fragments (733940+5201116 sites), 502 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     352 bp
  20% of sequence in fragments shorter than     437 bp
  25% of sequence in fragments shorter than     520 bp
  30% of sequence in fragments shorter than     604 bp
  35% of sequence in fragments shorter than     690 bp
  40% of sequence in fragments shorter than     779 bp
  45% of sequence in fragments shorter than     872 bp
  50% of sequence in fragments shorter than     967 bp
  55% of sequence in fragments shorter than    1072 bp
  60% of sequence in fragments shorter than    1183 bp
  65% of sequence in fragments shorter than    1312 bp
  70% of sequence in fragments shorter than    1447 bp
  75% of sequence in fragments shorter than    1616 bp
  80% of sequence in fragments shorter than    1818 bp
  85% of sequence in fragments shorter than    2082 bp
  90% of sequence in fragments shorter than    2456 bp
  95% of sequence in fragments shorter than    3124 bp

# AflII.BbvI (CTTAAG+GCAGC) double digest, 5932 thousand fragments (643284+5290051 sites), 502 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     269 bp
  15% of sequence in fragments shorter than     358 bp
  20% of sequence in fragments shorter than     449 bp
  25% of sequence in fragments shorter than     539 bp
  30% of sequence in fragments shorter than     632 bp
  35% of sequence in fragments shorter than     727 bp
  40% of sequence in fragments shorter than     826 bp
  45% of sequence in fragments shorter than     927 bp
  50% of sequence in fragments shorter than    1038 bp
  55% of sequence in fragments shorter than    1154 bp
  60% of sequence in fragments shorter than    1285 bp
  65% of sequence in fragments shorter than    1431 bp
  70% of sequence in fragments shorter than    1600 bp
  75% of sequence in fragments shorter than    1793 bp
  80% of sequence in fragments shorter than    2037 bp
  85% of sequence in fragments shorter than    2330 bp
  90% of sequence in fragments shorter than    2756 bp
  95% of sequence in fragments shorter than    3489 bp

# BbvI.HaeII (GCAGC+RGCGCY) double digest, 5919 thousand fragments (5290051+630101 sites), 503 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     558 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     761 bp
  40% of sequence in fragments shorter than     867 bp
  45% of sequence in fragments shorter than     982 bp
  50% of sequence in fragments shorter than    1104 bp
  55% of sequence in fragments shorter than    1242 bp
  60% of sequence in fragments shorter than    1391 bp
  65% of sequence in fragments shorter than    1559 bp
  70% of sequence in fragments shorter than    1738 bp
  75% of sequence in fragments shorter than    1954 bp
  80% of sequence in fragments shorter than    2212 bp
  85% of sequence in fragments shorter than    2559 bp
  90% of sequence in fragments shorter than    3043 bp
  95% of sequence in fragments shorter than    3876 bp

# AvaIII.TfiI (ATGCAT+GAWTC) double digest, 5917 thousand fragments (933357+4984362 sites), 503 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     401 bp
  25% of sequence in fragments shorter than     477 bp
  30% of sequence in fragments shorter than     550 bp
  35% of sequence in fragments shorter than     626 bp
  40% of sequence in fragments shorter than     699 bp
  45% of sequence in fragments shorter than     779 bp
  50% of sequence in fragments shorter than     860 bp
  55% of sequence in fragments shorter than     951 bp
  60% of sequence in fragments shorter than    1048 bp
  65% of sequence in fragments shorter than    1157 bp
  70% of sequence in fragments shorter than    1280 bp
  75% of sequence in fragments shorter than    1423 bp
  80% of sequence in fragments shorter than    1595 bp
  85% of sequence in fragments shorter than    1811 bp
  90% of sequence in fragments shorter than    2107 bp
  95% of sequence in fragments shorter than    2612 bp

# BsaAI.FokI (YACGTR+GGATG) double digest, 5914 thousand fragments (713801+5201116 sites), 503 bp average fragment length
   5% of sequence in fragments shorter than     172 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     426 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     663 bp
  40% of sequence in fragments shorter than     746 bp
  45% of sequence in fragments shorter than     833 bp
  50% of sequence in fragments shorter than     925 bp
  55% of sequence in fragments shorter than    1019 bp
  60% of sequence in fragments shorter than    1121 bp
  65% of sequence in fragments shorter than    1237 bp
  70% of sequence in fragments shorter than    1369 bp
  75% of sequence in fragments shorter than    1519 bp
  80% of sequence in fragments shorter than    1705 bp
  85% of sequence in fragments shorter than    1939 bp
  90% of sequence in fragments shorter than    2275 bp
  95% of sequence in fragments shorter than    2868 bp

# AclI.BsrI (AACGTT+ACTGG) double digest, 5913 thousand fragments (172706+5741310 sites), 503 bp average fragment length
   5% of sequence in fragments shorter than     172 bp
  10% of sequence in fragments shorter than     260 bp
  15% of sequence in fragments shorter than     341 bp
  20% of sequence in fragments shorter than     417 bp
  25% of sequence in fragments shorter than     491 bp
  30% of sequence in fragments shorter than     567 bp
  35% of sequence in fragments shorter than     643 bp
  40% of sequence in fragments shorter than     714 bp
  45% of sequence in fragments shorter than     796 bp
  50% of sequence in fragments shorter than     881 bp
  55% of sequence in fragments shorter than     975 bp
  60% of sequence in fragments shorter than    1077 bp
  65% of sequence in fragments shorter than    1192 bp
  70% of sequence in fragments shorter than    1322 bp
  75% of sequence in fragments shorter than    1469 bp
  80% of sequence in fragments shorter than    1649 bp
  85% of sequence in fragments shorter than    1882 bp
  90% of sequence in fragments shorter than    2209 bp
  95% of sequence in fragments shorter than    2792 bp

# AciI.BfiI (CCGC+ACTGGG) double digest, 5912 thousand fragments (4206130+1706594 sites), 504 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     278 bp
  15% of sequence in fragments shorter than     390 bp
  20% of sequence in fragments shorter than     505 bp
  25% of sequence in fragments shorter than     620 bp
  30% of sequence in fragments shorter than     739 bp
  35% of sequence in fragments shorter than     859 bp
  40% of sequence in fragments shorter than     985 bp
  45% of sequence in fragments shorter than    1123 bp
  50% of sequence in fragments shorter than    1273 bp
  55% of sequence in fragments shorter than    1437 bp
  60% of sequence in fragments shorter than    1617 bp
  65% of sequence in fragments shorter than    1822 bp
  70% of sequence in fragments shorter than    2058 bp
  75% of sequence in fragments shorter than    2335 bp
  80% of sequence in fragments shorter than    2673 bp
  85% of sequence in fragments shorter than    3105 bp
  90% of sequence in fragments shorter than    3731 bp
  95% of sequence in fragments shorter than    4832 bp

# StuI.TfiI (AGGCCT+GAWTC) double digest, 5909 thousand fragments (925729+4984362 sites), 504 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     258 bp
  15% of sequence in fragments shorter than     335 bp
  20% of sequence in fragments shorter than     407 bp
  25% of sequence in fragments shorter than     485 bp
  30% of sequence in fragments shorter than     558 bp
  35% of sequence in fragments shorter than     635 bp
  40% of sequence in fragments shorter than     712 bp
  45% of sequence in fragments shorter than     793 bp
  50% of sequence in fragments shorter than     879 bp
  55% of sequence in fragments shorter than     973 bp
  60% of sequence in fragments shorter than    1070 bp
  65% of sequence in fragments shorter than    1180 bp
  70% of sequence in fragments shorter than    1304 bp
  75% of sequence in fragments shorter than    1448 bp
  80% of sequence in fragments shorter than    1620 bp
  85% of sequence in fragments shorter than    1836 bp
  90% of sequence in fragments shorter than    2128 bp
  95% of sequence in fragments shorter than    2621 bp

# SfeI.SspI (CTRYAG+AATATT) double digest, 5907 thousand fragments (3531009+2377267 sites), 504 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     264 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     412 bp
  25% of sequence in fragments shorter than     482 bp
  30% of sequence in fragments shorter than     551 bp
  35% of sequence in fragments shorter than     625 bp
  40% of sequence in fragments shorter than     697 bp
  45% of sequence in fragments shorter than     777 bp
  50% of sequence in fragments shorter than     861 bp
  55% of sequence in fragments shorter than     951 bp
  60% of sequence in fragments shorter than    1045 bp
  65% of sequence in fragments shorter than    1154 bp
  70% of sequence in fragments shorter than    1276 bp
  75% of sequence in fragments shorter than    1416 bp
  80% of sequence in fragments shorter than    1584 bp
  85% of sequence in fragments shorter than    1801 bp
  90% of sequence in fragments shorter than    2102 bp
  95% of sequence in fragments shorter than    2596 bp

# BceAI.FokI (ACGGC+GGATG) double digest, 5905 thousand fragments (705178+5201116 sites), 504 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     427 bp
  25% of sequence in fragments shorter than     507 bp
  30% of sequence in fragments shorter than     587 bp
  35% of sequence in fragments shorter than     669 bp
  40% of sequence in fragments shorter than     754 bp
  45% of sequence in fragments shorter than     844 bp
  50% of sequence in fragments shorter than     935 bp
  55% of sequence in fragments shorter than    1038 bp
  60% of sequence in fragments shorter than    1147 bp
  65% of sequence in fragments shorter than    1268 bp
  70% of sequence in fragments shorter than    1399 bp
  75% of sequence in fragments shorter than    1561 bp
  80% of sequence in fragments shorter than    1756 bp
  85% of sequence in fragments shorter than    2011 bp
  90% of sequence in fragments shorter than    2369 bp
  95% of sequence in fragments shorter than    3007 bp

# BsrI.PacI (ACTGG+TTAATTAA) double digest, 5900 thousand fragments (5741310+159455 sites), 505 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     261 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     420 bp
  25% of sequence in fragments shorter than     493 bp
  30% of sequence in fragments shorter than     568 bp
  35% of sequence in fragments shorter than     645 bp
  40% of sequence in fragments shorter than     715 bp
  45% of sequence in fragments shorter than     797 bp
  50% of sequence in fragments shorter than     881 bp
  55% of sequence in fragments shorter than     976 bp
  60% of sequence in fragments shorter than    1077 bp
  65% of sequence in fragments shorter than    1191 bp
  70% of sequence in fragments shorter than    1321 bp
  75% of sequence in fragments shorter than    1468 bp
  80% of sequence in fragments shorter than    1649 bp
  85% of sequence in fragments shorter than    1883 bp
  90% of sequence in fragments shorter than    2207 bp
  95% of sequence in fragments shorter than    2790 bp

# BsrI.Hpy99I (ACTGG+CGWCG) double digest, 5898 thousand fragments (5741310+157917 sites), 505 bp average fragment length
   5% of sequence in fragments shorter than     172 bp
  10% of sequence in fragments shorter than     261 bp
  15% of sequence in fragments shorter than     341 bp
  20% of sequence in fragments shorter than     420 bp
  25% of sequence in fragments shorter than     493 bp
  30% of sequence in fragments shorter than     569 bp
  35% of sequence in fragments shorter than     647 bp
  40% of sequence in fragments shorter than     718 bp
  45% of sequence in fragments shorter than     802 bp
  50% of sequence in fragments shorter than     887 bp
  55% of sequence in fragments shorter than     983 bp
  60% of sequence in fragments shorter than    1087 bp
  65% of sequence in fragments shorter than    1203 bp
  70% of sequence in fragments shorter than    1336 bp
  75% of sequence in fragments shorter than    1486 bp
  80% of sequence in fragments shorter than    1672 bp
  85% of sequence in fragments shorter than    1908 bp
  90% of sequence in fragments shorter than    2242 bp
  95% of sequence in fragments shorter than    2843 bp

# BciVI.FokI (GTATCC+GGATG) double digest, 5896 thousand fragments (696093+5201116 sites), 505 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     426 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     584 bp
  35% of sequence in fragments shorter than     663 bp
  40% of sequence in fragments shorter than     746 bp
  45% of sequence in fragments shorter than     831 bp
  50% of sequence in fragments shorter than     923 bp
  55% of sequence in fragments shorter than    1017 bp
  60% of sequence in fragments shorter than    1120 bp
  65% of sequence in fragments shorter than    1233 bp
  70% of sequence in fragments shorter than    1366 bp
  75% of sequence in fragments shorter than    1516 bp
  80% of sequence in fragments shorter than    1705 bp
  85% of sequence in fragments shorter than    1938 bp
  90% of sequence in fragments shorter than    2277 bp
  95% of sequence in fragments shorter than    2872 bp

# BbvI.SacI (GCAGC+GAGCTC) double digest, 5892 thousand fragments (5290051+603323 sites), 505 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     455 bp
  25% of sequence in fragments shorter than     553 bp
  30% of sequence in fragments shorter than     651 bp
  35% of sequence in fragments shorter than     754 bp
  40% of sequence in fragments shorter than     858 bp
  45% of sequence in fragments shorter than     968 bp
  50% of sequence in fragments shorter than    1088 bp
  55% of sequence in fragments shorter than    1222 bp
  60% of sequence in fragments shorter than    1368 bp
  65% of sequence in fragments shorter than    1531 bp
  70% of sequence in fragments shorter than    1706 bp
  75% of sequence in fragments shorter than    1911 bp
  80% of sequence in fragments shorter than    2170 bp
  85% of sequence in fragments shorter than    2511 bp
  90% of sequence in fragments shorter than    2979 bp
  95% of sequence in fragments shorter than    3812 bp

# BglII.CviQI (AGATCT+GTAC) double digest, 5889 thousand fragments (774732+5115078 sites), 506 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     424 bp
  25% of sequence in fragments shorter than     497 bp
  30% of sequence in fragments shorter than     566 bp
  35% of sequence in fragments shorter than     641 bp
  40% of sequence in fragments shorter than     717 bp
  45% of sequence in fragments shorter than     797 bp
  50% of sequence in fragments shorter than     883 bp
  55% of sequence in fragments shorter than     976 bp
  60% of sequence in fragments shorter than    1073 bp
  65% of sequence in fragments shorter than    1186 bp
  70% of sequence in fragments shorter than    1305 bp
  75% of sequence in fragments shorter than    1437 bp
  80% of sequence in fragments shorter than    1614 bp
  85% of sequence in fragments shorter than    1841 bp
  90% of sequence in fragments shorter than    2162 bp
  95% of sequence in fragments shorter than    2749 bp

# NdeI.TfiI (CATATG+GAWTC) double digest, 5887 thousand fragments (903360+4984362 sites), 506 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     340 bp
  20% of sequence in fragments shorter than     404 bp
  25% of sequence in fragments shorter than     480 bp
  30% of sequence in fragments shorter than     552 bp
  35% of sequence in fragments shorter than     629 bp
  40% of sequence in fragments shorter than     704 bp
  45% of sequence in fragments shorter than     785 bp
  50% of sequence in fragments shorter than     867 bp
  55% of sequence in fragments shorter than     960 bp
  60% of sequence in fragments shorter than    1059 bp
  65% of sequence in fragments shorter than    1170 bp
  70% of sequence in fragments shorter than    1296 bp
  75% of sequence in fragments shorter than    1443 bp
  80% of sequence in fragments shorter than    1620 bp
  85% of sequence in fragments shorter than    1840 bp
  90% of sequence in fragments shorter than    2145 bp
  95% of sequence in fragments shorter than    2664 bp

# AvrII.BbvI (CCTAGG+GCAGC) double digest, 5884 thousand fragments (594956+5290051 sites), 506 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     270 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     455 bp
  25% of sequence in fragments shorter than     552 bp
  30% of sequence in fragments shorter than     646 bp
  35% of sequence in fragments shorter than     740 bp
  40% of sequence in fragments shorter than     844 bp
  45% of sequence in fragments shorter than     952 bp
  50% of sequence in fragments shorter than    1070 bp
  55% of sequence in fragments shorter than    1203 bp
  60% of sequence in fragments shorter than    1339 bp
  65% of sequence in fragments shorter than    1498 bp
  70% of sequence in fragments shorter than    1682 bp
  75% of sequence in fragments shorter than    1894 bp
  80% of sequence in fragments shorter than    2142 bp
  85% of sequence in fragments shorter than    2477 bp
  90% of sequence in fragments shorter than    2934 bp
  95% of sequence in fragments shorter than    3750 bp

# CviQI.NcoI (GTAC+CCATGG) double digest, 5874 thousand fragments (5115078+759594 sites), 507 bp average fragment length
   5% of sequence in fragments shorter than     178 bp
  10% of sequence in fragments shorter than     271 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     501 bp
  30% of sequence in fragments shorter than     574 bp
  35% of sequence in fragments shorter than     650 bp
  40% of sequence in fragments shorter than     726 bp
  45% of sequence in fragments shorter than     803 bp
  50% of sequence in fragments shorter than     890 bp
  55% of sequence in fragments shorter than     981 bp
  60% of sequence in fragments shorter than    1070 bp
  65% of sequence in fragments shorter than    1181 bp
  70% of sequence in fragments shorter than    1298 bp
  75% of sequence in fragments shorter than    1437 bp
  80% of sequence in fragments shorter than    1608 bp
  85% of sequence in fragments shorter than    1830 bp
  90% of sequence in fragments shorter than    2141 bp
  95% of sequence in fragments shorter than    2693 bp

# BsrI.SnaBI (ACTGG+TACGTA) double digest, 5873 thousand fragments (5741310+132299 sites), 507 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     421 bp
  25% of sequence in fragments shorter than     495 bp
  30% of sequence in fragments shorter than     571 bp
  35% of sequence in fragments shorter than     648 bp
  40% of sequence in fragments shorter than     718 bp
  45% of sequence in fragments shorter than     802 bp
  50% of sequence in fragments shorter than     886 bp
  55% of sequence in fragments shorter than     982 bp
  60% of sequence in fragments shorter than    1084 bp
  65% of sequence in fragments shorter than    1199 bp
  70% of sequence in fragments shorter than    1329 bp
  75% of sequence in fragments shorter than    1474 bp
  80% of sequence in fragments shorter than    1656 bp
  85% of sequence in fragments shorter than    1890 bp
  90% of sequence in fragments shorter than    2216 bp
  95% of sequence in fragments shorter than    2792 bp

# BsrI.NaeI (ACTGG+GCCGGC) double digest, 5866 thousand fragments (5741310+125692 sites), 507 bp average fragment length
   5% of sequence in fragments shorter than     172 bp
  10% of sequence in fragments shorter than     262 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     422 bp
  25% of sequence in fragments shorter than     496 bp
  30% of sequence in fragments shorter than     573 bp
  35% of sequence in fragments shorter than     650 bp
  40% of sequence in fragments shorter than     722 bp
  45% of sequence in fragments shorter than     806 bp
  50% of sequence in fragments shorter than     892 bp
  55% of sequence in fragments shorter than     989 bp
  60% of sequence in fragments shorter than    1093 bp
  65% of sequence in fragments shorter than    1210 bp
  70% of sequence in fragments shorter than    1344 bp
  75% of sequence in fragments shorter than    1495 bp
  80% of sequence in fragments shorter than    1682 bp
  85% of sequence in fragments shorter than    1919 bp
  90% of sequence in fragments shorter than    2254 bp
  95% of sequence in fragments shorter than    2862 bp

# BsrI.XhoI (ACTGG+CTCGAG) double digest, 5862 thousand fragments (5741310+121308 sites), 508 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     263 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     421 bp
  25% of sequence in fragments shorter than     495 bp
  30% of sequence in fragments shorter than     571 bp
  35% of sequence in fragments shorter than     649 bp
  40% of sequence in fragments shorter than     720 bp
  45% of sequence in fragments shorter than     804 bp
  50% of sequence in fragments shorter than     890 bp
  55% of sequence in fragments shorter than     987 bp
  60% of sequence in fragments shorter than    1090 bp
  65% of sequence in fragments shorter than    1206 bp
  70% of sequence in fragments shorter than    1339 bp
  75% of sequence in fragments shorter than    1489 bp
  80% of sequence in fragments shorter than    1675 bp
  85% of sequence in fragments shorter than    1910 bp
  90% of sequence in fragments shorter than    2244 bp
  95% of sequence in fragments shorter than    2846 bp

# BbvI.HgaI (GCAGC+GACGC) double digest, 5861 thousand fragments (5290051+571889 sites), 508 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     271 bp
  15% of sequence in fragments shorter than     367 bp
  20% of sequence in fragments shorter than     467 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     671 bp
  35% of sequence in fragments shorter than     778 bp
  40% of sequence in fragments shorter than     884 bp
  45% of sequence in fragments shorter than    1002 bp
  50% of sequence in fragments shorter than    1128 bp
  55% of sequence in fragments shorter than    1265 bp
  60% of sequence in fragments shorter than    1415 bp
  65% of sequence in fragments shorter than    1585 bp
  70% of sequence in fragments shorter than    1766 bp
  75% of sequence in fragments shorter than    1990 bp
  80% of sequence in fragments shorter than    2254 bp
  85% of sequence in fragments shorter than    2609 bp
  90% of sequence in fragments shorter than    3101 bp
  95% of sequence in fragments shorter than    3961 bp

# AvaII.StyI (GGWCC+CCWWGG) double digest, 5861 thousand fragments (2747690+3114107 sites), 508 bp average fragment length
   5% of sequence in fragments shorter than     169 bp
  10% of sequence in fragments shorter than     269 bp
  15% of sequence in fragments shorter than     352 bp
  20% of sequence in fragments shorter than     439 bp
  25% of sequence in fragments shorter than     522 bp
  30% of sequence in fragments shorter than     611 bp
  35% of sequence in fragments shorter than     700 bp
  40% of sequence in fragments shorter than     790 bp
  45% of sequence in fragments shorter than     891 bp
  50% of sequence in fragments shorter than     990 bp
  55% of sequence in fragments shorter than    1108 bp
  60% of sequence in fragments shorter than    1240 bp
  65% of sequence in fragments shorter than    1382 bp
  70% of sequence in fragments shorter than    1550 bp
  75% of sequence in fragments shorter than    1750 bp
  80% of sequence in fragments shorter than    1992 bp
  85% of sequence in fragments shorter than    2300 bp
  90% of sequence in fragments shorter than    2752 bp
  95% of sequence in fragments shorter than    3571 bp

# BbvI.EciI (GCAGC+GGCGGA) double digest, 5860 thousand fragments (5290051+571273 sites), 508 bp average fragment length
   5% of sequence in fragments shorter than     165 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     359 bp
  20% of sequence in fragments shorter than     453 bp
  25% of sequence in fragments shorter than     548 bp
  30% of sequence in fragments shorter than     644 bp
  35% of sequence in fragments shorter than     745 bp
  40% of sequence in fragments shorter than     849 bp
  45% of sequence in fragments shorter than     957 bp
  50% of sequence in fragments shorter than    1077 bp
  55% of sequence in fragments shorter than    1210 bp
  60% of sequence in fragments shorter than    1354 bp
  65% of sequence in fragments shorter than    1516 bp
  70% of sequence in fragments shorter than    1691 bp
  75% of sequence in fragments shorter than    1907 bp
  80% of sequence in fragments shorter than    2158 bp
  85% of sequence in fragments shorter than    2501 bp
  90% of sequence in fragments shorter than    2985 bp
  95% of sequence in fragments shorter than    3831 bp

# BbvI.MfeI (GCAGC+CAATTG) double digest, 5853 thousand fragments (5290051+564303 sites), 509 bp average fragment length
   5% of sequence in fragments shorter than     172 bp
  10% of sequence in fragments shorter than     273 bp
  15% of sequence in fragments shorter than     364 bp
  20% of sequence in fragments shorter than     456 bp
  25% of sequence in fragments shorter than     550 bp
  30% of sequence in fragments shorter than     641 bp
  35% of sequence in fragments shorter than     738 bp
  40% of sequence in fragments shorter than     837 bp
  45% of sequence in fragments shorter than     939 bp
  50% of sequence in fragments shorter than    1044 bp
  55% of sequence in fragments shorter than    1169 bp
  60% of sequence in fragments shorter than    1300 bp
  65% of sequence in fragments shorter than    1446 bp
  70% of sequence in fragments shorter than    1616 bp
  75% of sequence in fragments shorter than    1809 bp
  80% of sequence in fragments shorter than    2049 bp
  85% of sequence in fragments shorter than    2341 bp
  90% of sequence in fragments shorter than    2756 bp
  95% of sequence in fragments shorter than    3460 bp

# AciI.PsiI (CCGC+TTATAA) double digest, 5853 thousand fragments (4206130+1647912 sites), 509 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     291 bp
  15% of sequence in fragments shorter than     399 bp
  20% of sequence in fragments shorter than     504 bp
  25% of sequence in fragments shorter than     606 bp
  30% of sequence in fragments shorter than     708 bp
  35% of sequence in fragments shorter than     813 bp
  40% of sequence in fragments shorter than     917 bp
  45% of sequence in fragments shorter than    1031 bp
  50% of sequence in fragments shorter than    1150 bp
  55% of sequence in fragments shorter than    1280 bp
  60% of sequence in fragments shorter than    1419 bp
  65% of sequence in fragments shorter than    1575 bp
  70% of sequence in fragments shorter than    1754 bp
  75% of sequence in fragments shorter than    1958 bp
  80% of sequence in fragments shorter than    2209 bp
  85% of sequence in fragments shorter than    2525 bp
  90% of sequence in fragments shorter than    2968 bp
  95% of sequence in fragments shorter than    3756 bp

# BclI.CviQI (TGATCA+GTAC) double digest, 5853 thousand fragments (738785+5115078 sites), 509 bp average fragment length
   5% of sequence in fragments shorter than     177 bp
  10% of sequence in fragments shorter than     269 bp
  15% of sequence in fragments shorter than     351 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     502 bp
  30% of sequence in fragments shorter than     573 bp
  35% of sequence in fragments shorter than     649 bp
  40% of sequence in fragments shorter than     723 bp
  45% of sequence in fragments shorter than     804 bp
  50% of sequence in fragments shorter than     889 bp
  55% of sequence in fragments shorter than     981 bp
  60% of sequence in fragments shorter than    1077 bp
  65% of sequence in fragments shorter than    1190 bp
  70% of sequence in fragments shorter than    1313 bp
  75% of sequence in fragments shorter than    1447 bp
  80% of sequence in fragments shorter than    1622 bp
  85% of sequence in fragments shorter than    1852 bp
  90% of sequence in fragments shorter than    2171 bp
  95% of sequence in fragments shorter than    2754 bp

# AvaII.NspI (GGWCC+RCATGY) double digest, 5851 thousand fragments (2747690+3104359 sites), 509 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     346 bp
  20% of sequence in fragments shorter than     423 bp
  25% of sequence in fragments shorter than     502 bp
  30% of sequence in fragments shorter than     580 bp
  35% of sequence in fragments shorter than     660 bp
  40% of sequence in fragments shorter than     740 bp
  45% of sequence in fragments shorter than     828 bp
  50% of sequence in fragments shorter than     918 bp
  55% of sequence in fragments shorter than    1013 bp
  60% of sequence in fragments shorter than    1122 bp
  65% of sequence in fragments shorter than    1243 bp
  70% of sequence in fragments shorter than    1371 bp
  75% of sequence in fragments shorter than    1526 bp
  80% of sequence in fragments shorter than    1719 bp
  85% of sequence in fragments shorter than    1962 bp
  90% of sequence in fragments shorter than    2311 bp
  95% of sequence in fragments shorter than    2914 bp

# CviQI.FnuDII (GTAC+CGCG) double digest, 5848 thousand fragments (5115078+733940 sites), 509 bp average fragment length
   5% of sequence in fragments shorter than     179 bp
  10% of sequence in fragments shorter than     275 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     441 bp
  25% of sequence in fragments shorter than     515 bp
  30% of sequence in fragments shorter than     592 bp
  35% of sequence in fragments shorter than     670 bp
  40% of sequence in fragments shorter than     748 bp
  45% of sequence in fragments shorter than     830 bp
  50% of sequence in fragments shorter than     918 bp
  55% of sequence in fragments shorter than    1013 bp
  60% of sequence in fragments shorter than    1114 bp
  65% of sequence in fragments shorter than    1224 bp
  70% of sequence in fragments shorter than    1348 bp
  75% of sequence in fragments shorter than    1484 bp
  80% of sequence in fragments shorter than    1659 bp
  85% of sequence in fragments shorter than    1888 bp
  90% of sequence in fragments shorter than    2200 bp
  95% of sequence in fragments shorter than    2768 bp

# HpaII.SfeI (CCGG+CTRYAG) double digest, 5848 thousand fragments (2317722+3531009 sites), 509 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     361 bp
  20% of sequence in fragments shorter than     451 bp
  25% of sequence in fragments shorter than     536 bp
  30% of sequence in fragments shorter than     626 bp
  35% of sequence in fragments shorter than     714 bp
  40% of sequence in fragments shorter than     810 bp
  45% of sequence in fragments shorter than     913 bp
  50% of sequence in fragments shorter than    1022 bp
  55% of sequence in fragments shorter than    1133 bp
  60% of sequence in fragments shorter than    1261 bp
  65% of sequence in fragments shorter than    1403 bp
  70% of sequence in fragments shorter than    1568 bp
  75% of sequence in fragments shorter than    1760 bp
  80% of sequence in fragments shorter than    1989 bp
  85% of sequence in fragments shorter than    2265 bp
  90% of sequence in fragments shorter than    2685 bp
  95% of sequence in fragments shorter than    3420 bp

# HindIII.TfiI (AAGCTT+GAWTC) double digest, 5844 thousand fragments (860365+4984362 sites), 509 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     406 bp
  25% of sequence in fragments shorter than     483 bp
  30% of sequence in fragments shorter than     558 bp
  35% of sequence in fragments shorter than     633 bp
  40% of sequence in fragments shorter than     706 bp
  45% of sequence in fragments shorter than     788 bp
  50% of sequence in fragments shorter than     874 bp
  55% of sequence in fragments shorter than     967 bp
  60% of sequence in fragments shorter than    1066 bp
  65% of sequence in fragments shorter than    1176 bp
  70% of sequence in fragments shorter than    1302 bp
  75% of sequence in fragments shorter than    1446 bp
  80% of sequence in fragments shorter than    1622 bp
  85% of sequence in fragments shorter than    1840 bp
  90% of sequence in fragments shorter than    2140 bp
  95% of sequence in fragments shorter than    2652 bp

# AflII.FokI (CTTAAG+GGATG) double digest, 5843 thousand fragments (643284+5201116 sites), 509 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     269 bp
  15% of sequence in fragments shorter than     350 bp
  20% of sequence in fragments shorter than     429 bp
  25% of sequence in fragments shorter than     508 bp
  30% of sequence in fragments shorter than     586 bp
  35% of sequence in fragments shorter than     665 bp
  40% of sequence in fragments shorter than     747 bp
  45% of sequence in fragments shorter than     833 bp
  50% of sequence in fragments shorter than     924 bp
  55% of sequence in fragments shorter than    1018 bp
  60% of sequence in fragments shorter than    1121 bp
  65% of sequence in fragments shorter than    1233 bp
  70% of sequence in fragments shorter than    1363 bp
  75% of sequence in fragments shorter than    1509 bp
  80% of sequence in fragments shorter than    1692 bp
  85% of sequence in fragments shorter than    1922 bp
  90% of sequence in fragments shorter than    2257 bp
  95% of sequence in fragments shorter than    2857 bp

# SmlI.StyI (CTYRAG+CCWWGG) double digest, 5841 thousand fragments (2727867+3114107 sites), 510 bp average fragment length
   5% of sequence in fragments shorter than     169 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     346 bp
  20% of sequence in fragments shorter than     424 bp
  25% of sequence in fragments shorter than     497 bp
  30% of sequence in fragments shorter than     571 bp
  35% of sequence in fragments shorter than     647 bp
  40% of sequence in fragments shorter than     727 bp
  45% of sequence in fragments shorter than     809 bp
  50% of sequence in fragments shorter than     900 bp
  55% of sequence in fragments shorter than     998 bp
  60% of sequence in fragments shorter than    1104 bp
  65% of sequence in fragments shorter than    1225 bp
  70% of sequence in fragments shorter than    1363 bp
  75% of sequence in fragments shorter than    1522 bp
  80% of sequence in fragments shorter than    1723 bp
  85% of sequence in fragments shorter than    1986 bp
  90% of sequence in fragments shorter than    2362 bp
  95% of sequence in fragments shorter than    2985 bp

# BbvI.SphI (GCAGC+GCATGC) double digest, 5840 thousand fragments (5290051+550952 sites), 510 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     273 bp
  15% of sequence in fragments shorter than     366 bp
  20% of sequence in fragments shorter than     460 bp
  25% of sequence in fragments shorter than     557 bp
  30% of sequence in fragments shorter than     655 bp
  35% of sequence in fragments shorter than     756 bp
  40% of sequence in fragments shorter than     860 bp
  45% of sequence in fragments shorter than     970 bp
  50% of sequence in fragments shorter than    1089 bp
  55% of sequence in fragments shorter than    1221 bp
  60% of sequence in fragments shorter than    1364 bp
  65% of sequence in fragments shorter than    1525 bp
  70% of sequence in fragments shorter than    1698 bp
  75% of sequence in fragments shorter than    1910 bp
  80% of sequence in fragments shorter than    2155 bp
  85% of sequence in fragments shorter than    2493 bp
  90% of sequence in fragments shorter than    2958 bp
  95% of sequence in fragments shorter than    3775 bp

# TfiI.XbaI (GAWTC+TCTAGA) double digest, 5834 thousand fragments (4984362+850998 sites), 510 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     272 bp
  15% of sequence in fragments shorter than     343 bp
  20% of sequence in fragments shorter than     414 bp
  25% of sequence in fragments shorter than     491 bp
  30% of sequence in fragments shorter than     569 bp
  35% of sequence in fragments shorter than     648 bp
  40% of sequence in fragments shorter than     725 bp
  45% of sequence in fragments shorter than     806 bp
  50% of sequence in fragments shorter than     894 bp
  55% of sequence in fragments shorter than     990 bp
  60% of sequence in fragments shorter than    1092 bp
  65% of sequence in fragments shorter than    1206 bp
  70% of sequence in fragments shorter than    1333 bp
  75% of sequence in fragments shorter than    1482 bp
  80% of sequence in fragments shorter than    1661 bp
  85% of sequence in fragments shorter than    1884 bp
  90% of sequence in fragments shorter than    2193 bp
  95% of sequence in fragments shorter than    2717 bp

# BbvI.ScaI (GCAGC+AGTACT) double digest, 5833 thousand fragments (5290051+543793 sites), 510 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     273 bp
  15% of sequence in fragments shorter than     364 bp
  20% of sequence in fragments shorter than     457 bp
  25% of sequence in fragments shorter than     551 bp
  30% of sequence in fragments shorter than     645 bp
  35% of sequence in fragments shorter than     743 bp
  40% of sequence in fragments shorter than     843 bp
  45% of sequence in fragments shorter than     946 bp
  50% of sequence in fragments shorter than    1059 bp
  55% of sequence in fragments shorter than    1184 bp
  60% of sequence in fragments shorter than    1316 bp
  65% of sequence in fragments shorter than    1465 bp
  70% of sequence in fragments shorter than    1627 bp
  75% of sequence in fragments shorter than    1821 bp
  80% of sequence in fragments shorter than    2059 bp
  85% of sequence in fragments shorter than    2358 bp
  90% of sequence in fragments shorter than    2787 bp
  95% of sequence in fragments shorter than    3537 bp

# NspI.SmlI (RCATGY+CTYRAG) double digest, 5831 thousand fragments (3104359+2727867 sites), 510 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     424 bp
  25% of sequence in fragments shorter than     499 bp
  30% of sequence in fragments shorter than     572 bp
  35% of sequence in fragments shorter than     647 bp
  40% of sequence in fragments shorter than     723 bp
  45% of sequence in fragments shorter than     804 bp
  50% of sequence in fragments shorter than     890 bp
  55% of sequence in fragments shorter than     983 bp
  60% of sequence in fragments shorter than    1083 bp
  65% of sequence in fragments shorter than    1194 bp
  70% of sequence in fragments shorter than    1320 bp
  75% of sequence in fragments shorter than    1462 bp
  80% of sequence in fragments shorter than    1643 bp
  85% of sequence in fragments shorter than    1873 bp
  90% of sequence in fragments shorter than    2185 bp
  95% of sequence in fragments shorter than    2740 bp

# EcoRI.TfiI (GAATTC+GAWTC) double digest, 5831 thousand fragments (847401+4984362 sites), 511 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     342 bp
  20% of sequence in fragments shorter than     410 bp
  25% of sequence in fragments shorter than     488 bp
  30% of sequence in fragments shorter than     564 bp
  35% of sequence in fragments shorter than     643 bp
  40% of sequence in fragments shorter than     719 bp
  45% of sequence in fragments shorter than     800 bp
  50% of sequence in fragments shorter than     886 bp
  55% of sequence in fragments shorter than     981 bp
  60% of sequence in fragments shorter than    1081 bp
  65% of sequence in fragments shorter than    1193 bp
  70% of sequence in fragments shorter than    1320 bp
  75% of sequence in fragments shorter than    1466 bp
  80% of sequence in fragments shorter than    1641 bp
  85% of sequence in fragments shorter than    1863 bp
  90% of sequence in fragments shorter than    2169 bp
  95% of sequence in fragments shorter than    2687 bp

# FokI.HaeII (GGATG+RGCGCY) double digest, 5830 thousand fragments (5201116+630101 sites), 511 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     269 bp
  15% of sequence in fragments shorter than     350 bp
  20% of sequence in fragments shorter than     432 bp
  25% of sequence in fragments shorter than     514 bp
  30% of sequence in fragments shorter than     596 bp
  35% of sequence in fragments shorter than     681 bp
  40% of sequence in fragments shorter than     764 bp
  45% of sequence in fragments shorter than     856 bp
  50% of sequence in fragments shorter than     948 bp
  55% of sequence in fragments shorter than    1049 bp
  60% of sequence in fragments shorter than    1161 bp
  65% of sequence in fragments shorter than    1286 bp
  70% of sequence in fragments shorter than    1420 bp
  75% of sequence in fragments shorter than    1582 bp
  80% of sequence in fragments shorter than    1782 bp
  85% of sequence in fragments shorter than    2037 bp
  90% of sequence in fragments shorter than    2390 bp
  95% of sequence in fragments shorter than    3003 bp

# BsaAI.CviQI (YACGTR+GTAC) double digest, 5828 thousand fragments (713801+5115078 sites), 511 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     278 bp
  15% of sequence in fragments shorter than     361 bp
  20% of sequence in fragments shorter than     439 bp
  25% of sequence in fragments shorter than     512 bp
  30% of sequence in fragments shorter than     589 bp
  35% of sequence in fragments shorter than     666 bp
  40% of sequence in fragments shorter than     743 bp
  45% of sequence in fragments shorter than     825 bp
  50% of sequence in fragments shorter than     912 bp
  55% of sequence in fragments shorter than    1003 bp
  60% of sequence in fragments shorter than    1102 bp
  65% of sequence in fragments shorter than    1213 bp
  70% of sequence in fragments shorter than    1335 bp
  75% of sequence in fragments shorter than    1476 bp
  80% of sequence in fragments shorter than    1652 bp
  85% of sequence in fragments shorter than    1878 bp
  90% of sequence in fragments shorter than    2186 bp
  95% of sequence in fragments shorter than    2747 bp

# BsrI.ClaI (ACTGG+ATCGAT) double digest, 5827 thousand fragments (5741310+86477 sites), 511 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     264 bp
  15% of sequence in fragments shorter than     345 bp
  20% of sequence in fragments shorter than     425 bp
  25% of sequence in fragments shorter than     499 bp
  30% of sequence in fragments shorter than     576 bp
  35% of sequence in fragments shorter than     653 bp
  40% of sequence in fragments shorter than     725 bp
  45% of sequence in fragments shorter than     809 bp
  50% of sequence in fragments shorter than     894 bp
  55% of sequence in fragments shorter than     991 bp
  60% of sequence in fragments shorter than    1095 bp
  65% of sequence in fragments shorter than    1211 bp
  70% of sequence in fragments shorter than    1344 bp
  75% of sequence in fragments shorter than    1494 bp
  80% of sequence in fragments shorter than    1680 bp
  85% of sequence in fragments shorter than    1914 bp
  90% of sequence in fragments shorter than    2247 bp
  95% of sequence in fragments shorter than    2846 bp

# BceAI.CviQI (ACGGC+GTAC) double digest, 5819 thousand fragments (705178+5115078 sites), 512 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     274 bp
  15% of sequence in fragments shorter than     356 bp
  20% of sequence in fragments shorter than     434 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     579 bp
  35% of sequence in fragments shorter than     655 bp
  40% of sequence in fragments shorter than     731 bp
  45% of sequence in fragments shorter than     811 bp
  50% of sequence in fragments shorter than     895 bp
  55% of sequence in fragments shorter than     987 bp
  60% of sequence in fragments shorter than    1084 bp
  65% of sequence in fragments shorter than    1195 bp
  70% of sequence in fragments shorter than    1313 bp
  75% of sequence in fragments shorter than    1444 bp
  80% of sequence in fragments shorter than    1614 bp
  85% of sequence in fragments shorter than    1832 bp
  90% of sequence in fragments shorter than    2136 bp
  95% of sequence in fragments shorter than    2672 bp

# AatII.BsrI (GACGTC+ACTGG) double digest, 5811 thousand fragments (71089+5741310 sites), 512 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     346 bp
  20% of sequence in fragments shorter than     426 bp
  25% of sequence in fragments shorter than     501 bp
  30% of sequence in fragments shorter than     577 bp
  35% of sequence in fragments shorter than     655 bp
  40% of sequence in fragments shorter than     727 bp
  45% of sequence in fragments shorter than     812 bp
  50% of sequence in fragments shorter than     898 bp
  55% of sequence in fragments shorter than     996 bp
  60% of sequence in fragments shorter than    1100 bp
  65% of sequence in fragments shorter than    1217 bp
  70% of sequence in fragments shorter than    1351 bp
  75% of sequence in fragments shorter than    1502 bp
  80% of sequence in fragments shorter than    1689 bp
  85% of sequence in fragments shorter than    1926 bp
  90% of sequence in fragments shorter than    2262 bp
  95% of sequence in fragments shorter than    2869 bp

# BciVI.CviQI (GTATCC+GTAC) double digest, 5810 thousand fragments (696093+5115078 sites), 512 bp average fragment length
   5% of sequence in fragments shorter than     179 bp
  10% of sequence in fragments shorter than     272 bp
  15% of sequence in fragments shorter than     354 bp
  20% of sequence in fragments shorter than     433 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     580 bp
  35% of sequence in fragments shorter than     657 bp
  40% of sequence in fragments shorter than     733 bp
  45% of sequence in fragments shorter than     813 bp
  50% of sequence in fragments shorter than     901 bp
  55% of sequence in fragments shorter than     995 bp
  60% of sequence in fragments shorter than    1094 bp
  65% of sequence in fragments shorter than    1207 bp
  70% of sequence in fragments shorter than    1333 bp
  75% of sequence in fragments shorter than    1468 bp
  80% of sequence in fragments shorter than    1646 bp
  85% of sequence in fragments shorter than    1877 bp
  90% of sequence in fragments shorter than    2201 bp
  95% of sequence in fragments shorter than    2785 bp

# AciI.EsaBC3I (CCGC+TCGA) double digest, 5808 thousand fragments (4206130+1603346 sites), 512 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     296 bp
  15% of sequence in fragments shorter than     418 bp
  20% of sequence in fragments shorter than     538 bp
  25% of sequence in fragments shorter than     661 bp
  30% of sequence in fragments shorter than     783 bp
  35% of sequence in fragments shorter than     910 bp
  40% of sequence in fragments shorter than    1047 bp
  45% of sequence in fragments shorter than    1197 bp
  50% of sequence in fragments shorter than    1356 bp
  55% of sequence in fragments shorter than    1525 bp
  60% of sequence in fragments shorter than    1723 bp
  65% of sequence in fragments shorter than    1945 bp
  70% of sequence in fragments shorter than    2200 bp
  75% of sequence in fragments shorter than    2500 bp
  80% of sequence in fragments shorter than    2862 bp
  85% of sequence in fragments shorter than    3332 bp
  90% of sequence in fragments shorter than    3982 bp
  95% of sequence in fragments shorter than    5106 bp

# BsrI.SacII (ACTGG+CCGCGG) double digest, 5807 thousand fragments (5741310+66901 sites), 513 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     265 bp
  15% of sequence in fragments shorter than     346 bp
  20% of sequence in fragments shorter than     426 bp
  25% of sequence in fragments shorter than     501 bp
  30% of sequence in fragments shorter than     578 bp
  35% of sequence in fragments shorter than     657 bp
  40% of sequence in fragments shorter than     729 bp
  45% of sequence in fragments shorter than     814 bp
  50% of sequence in fragments shorter than     900 bp
  55% of sequence in fragments shorter than     998 bp
  60% of sequence in fragments shorter than    1103 bp
  65% of sequence in fragments shorter than    1220 bp
  70% of sequence in fragments shorter than    1356 bp
  75% of sequence in fragments shorter than    1507 bp
  80% of sequence in fragments shorter than    1696 bp
  85% of sequence in fragments shorter than    1934 bp
  90% of sequence in fragments shorter than    2272 bp
  95% of sequence in fragments shorter than    2885 bp

# FokI.SacI (GGATG+GAGCTC) double digest, 5803 thousand fragments (5201116+603323 sites), 513 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     271 bp
  15% of sequence in fragments shorter than     351 bp
  20% of sequence in fragments shorter than     432 bp
  25% of sequence in fragments shorter than     512 bp
  30% of sequence in fragments shorter than     593 bp
  35% of sequence in fragments shorter than     675 bp
  40% of sequence in fragments shorter than     760 bp
  45% of sequence in fragments shorter than     849 bp
  50% of sequence in fragments shorter than     938 bp
  55% of sequence in fragments shorter than    1038 bp
  60% of sequence in fragments shorter than    1147 bp
  65% of sequence in fragments shorter than    1268 bp
  70% of sequence in fragments shorter than    1400 bp
  75% of sequence in fragments shorter than    1560 bp
  80% of sequence in fragments shorter than    1752 bp
  85% of sequence in fragments shorter than    2002 bp
  90% of sequence in fragments shorter than    2361 bp
  95% of sequence in fragments shorter than    3009 bp

# BsrI.PspXI (ACTGG+VCTCGAGB) double digest, 5800 thousand fragments (5741310+59193 sites), 513 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     427 bp
  25% of sequence in fragments shorter than     502 bp
  30% of sequence in fragments shorter than     579 bp
  35% of sequence in fragments shorter than     657 bp
  40% of sequence in fragments shorter than     729 bp
  45% of sequence in fragments shorter than     814 bp
  50% of sequence in fragments shorter than     900 bp
  55% of sequence in fragments shorter than     998 bp
  60% of sequence in fragments shorter than    1102 bp
  65% of sequence in fragments shorter than    1219 bp
  70% of sequence in fragments shorter than    1354 bp
  75% of sequence in fragments shorter than    1505 bp
  80% of sequence in fragments shorter than    1693 bp
  85% of sequence in fragments shorter than    1930 bp
  90% of sequence in fragments shorter than    2266 bp
  95% of sequence in fragments shorter than    2875 bp

# AvrII.FokI (CCTAGG+GGATG) double digest, 5795 thousand fragments (594956+5201116 sites), 514 bp average fragment length
   5% of sequence in fragments shorter than     178 bp
  10% of sequence in fragments shorter than     274 bp
  15% of sequence in fragments shorter than     354 bp
  20% of sequence in fragments shorter than     436 bp
  25% of sequence in fragments shorter than     510 bp
  30% of sequence in fragments shorter than     591 bp
  35% of sequence in fragments shorter than     671 bp
  40% of sequence in fragments shorter than     756 bp
  45% of sequence in fragments shorter than     844 bp
  50% of sequence in fragments shorter than     939 bp
  55% of sequence in fragments shorter than    1041 bp
  60% of sequence in fragments shorter than    1150 bp
  65% of sequence in fragments shorter than    1268 bp
  70% of sequence in fragments shorter than    1398 bp
  75% of sequence in fragments shorter than    1557 bp
  80% of sequence in fragments shorter than    1751 bp
  85% of sequence in fragments shorter than    2003 bp
  90% of sequence in fragments shorter than    2359 bp
  95% of sequence in fragments shorter than    2998 bp

# AgeI.BsrI (ACCGGT+ACTGG) double digest, 5794 thousand fragments (53386+5741310 sites), 514 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     266 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     502 bp
  30% of sequence in fragments shorter than     579 bp
  35% of sequence in fragments shorter than     657 bp
  40% of sequence in fragments shorter than     729 bp
  45% of sequence in fragments shorter than     814 bp
  50% of sequence in fragments shorter than     901 bp
  55% of sequence in fragments shorter than     999 bp
  60% of sequence in fragments shorter than    1103 bp
  65% of sequence in fragments shorter than    1220 bp
  70% of sequence in fragments shorter than    1355 bp
  75% of sequence in fragments shorter than    1506 bp
  80% of sequence in fragments shorter than    1695 bp
  85% of sequence in fragments shorter than    1932 bp
  90% of sequence in fragments shorter than    2269 bp
  95% of sequence in fragments shorter than    2878 bp

# ApaLI.BbvI (GTGCAC+GCAGC) double digest, 5785 thousand fragments (496255+5290051 sites), 515 bp average fragment length
   5% of sequence in fragments shorter than     170 bp
  10% of sequence in fragments shorter than     274 bp
  15% of sequence in fragments shorter than     368 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     666 bp
  35% of sequence in fragments shorter than     770 bp
  40% of sequence in fragments shorter than     873 bp
  45% of sequence in fragments shorter than     990 bp
  50% of sequence in fragments shorter than    1112 bp
  55% of sequence in fragments shorter than    1247 bp
  60% of sequence in fragments shorter than    1393 bp
  65% of sequence in fragments shorter than    1561 bp
  70% of sequence in fragments shorter than    1739 bp
  75% of sequence in fragments shorter than    1955 bp
  80% of sequence in fragments shorter than    2209 bp
  85% of sequence in fragments shorter than    2557 bp
  90% of sequence in fragments shorter than    3030 bp
  95% of sequence in fragments shorter than    3865 bp

# BsrI.PmeI (ACTGG+GTTTAAAC) double digest, 5782 thousand fragments (5741310+42094 sites), 515 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     503 bp
  30% of sequence in fragments shorter than     580 bp
  35% of sequence in fragments shorter than     658 bp
  40% of sequence in fragments shorter than     730 bp
  45% of sequence in fragments shorter than     816 bp
  50% of sequence in fragments shorter than     902 bp
  55% of sequence in fragments shorter than     999 bp
  60% of sequence in fragments shorter than    1104 bp
  65% of sequence in fragments shorter than    1220 bp
  70% of sequence in fragments shorter than    1355 bp
  75% of sequence in fragments shorter than    1506 bp
  80% of sequence in fragments shorter than    1693 bp
  85% of sequence in fragments shorter than    1930 bp
  90% of sequence in fragments shorter than    2265 bp
  95% of sequence in fragments shorter than    2873 bp

# HhaI.Tsp45I (GCGC+GTSAC) double digest, 5777 thousand fragments (1756502+4021761 sites), 515 bp average fragment length
   5% of sequence in fragments shorter than     171 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     353 bp
  20% of sequence in fragments shorter than     437 bp
  25% of sequence in fragments shorter than     522 bp
  30% of sequence in fragments shorter than     607 bp
  35% of sequence in fragments shorter than     692 bp
  40% of sequence in fragments shorter than     785 bp
  45% of sequence in fragments shorter than     882 bp
  50% of sequence in fragments shorter than     986 bp
  55% of sequence in fragments shorter than    1098 bp
  60% of sequence in fragments shorter than    1223 bp
  65% of sequence in fragments shorter than    1365 bp
  70% of sequence in fragments shorter than    1528 bp
  75% of sequence in fragments shorter than    1722 bp
  80% of sequence in fragments shorter than    1960 bp
  85% of sequence in fragments shorter than    2274 bp
  90% of sequence in fragments shorter than    2733 bp
  95% of sequence in fragments shorter than    3564 bp

# FokI.HgaI (GGATG+GACGC) double digest, 5772 thousand fragments (5201116+571889 sites), 516 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     272 bp
  15% of sequence in fragments shorter than     354 bp
  20% of sequence in fragments shorter than     437 bp
  25% of sequence in fragments shorter than     519 bp
  30% of sequence in fragments shorter than     601 bp
  35% of sequence in fragments shorter than     686 bp
  40% of sequence in fragments shorter than     773 bp
  45% of sequence in fragments shorter than     864 bp
  50% of sequence in fragments shorter than     957 bp
  55% of sequence in fragments shorter than    1059 bp
  60% of sequence in fragments shorter than    1169 bp
  65% of sequence in fragments shorter than    1296 bp
  70% of sequence in fragments shorter than    1432 bp
  75% of sequence in fragments shorter than    1600 bp
  80% of sequence in fragments shorter than    1801 bp
  85% of sequence in fragments shorter than    2062 bp
  90% of sequence in fragments shorter than    2431 bp
  95% of sequence in fragments shorter than    3097 bp

# BsrI.SalI (ACTGG+GTCGAC) double digest, 5772 thousand fragments (5741310+31396 sites), 516 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     267 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     429 bp
  25% of sequence in fragments shorter than     504 bp
  30% of sequence in fragments shorter than     582 bp
  35% of sequence in fragments shorter than     660 bp
  40% of sequence in fragments shorter than     732 bp
  45% of sequence in fragments shorter than     818 bp
  50% of sequence in fragments shorter than     904 bp
  55% of sequence in fragments shorter than    1002 bp
  60% of sequence in fragments shorter than    1107 bp
  65% of sequence in fragments shorter than    1225 bp
  70% of sequence in fragments shorter than    1360 bp
  75% of sequence in fragments shorter than    1512 bp
  80% of sequence in fragments shorter than    1700 bp
  85% of sequence in fragments shorter than    1938 bp
  90% of sequence in fragments shorter than    2276 bp
  95% of sequence in fragments shorter than    2888 bp

# EciI.FokI (GGCGGA+GGATG) double digest, 5771 thousand fragments (571273+5201116 sites), 516 bp average fragment length
   5% of sequence in fragments shorter than     172 bp
  10% of sequence in fragments shorter than     272 bp
  15% of sequence in fragments shorter than     354 bp
  20% of sequence in fragments shorter than     437 bp
  25% of sequence in fragments shorter than     518 bp
  30% of sequence in fragments shorter than     599 bp
  35% of sequence in fragments shorter than     683 bp
  40% of sequence in fragments shorter than     769 bp
  45% of sequence in fragments shorter than     860 bp
  50% of sequence in fragments shorter than     952 bp
  55% of sequence in fragments shorter than    1054 bp
  60% of sequence in fragments shorter than    1165 bp
  65% of sequence in fragments shorter than    1287 bp
  70% of sequence in fragments shorter than    1418 bp
  75% of sequence in fragments shorter than    1581 bp
  80% of sequence in fragments shorter than    1778 bp
  85% of sequence in fragments shorter than    2035 bp
  90% of sequence in fragments shorter than    2402 bp
  95% of sequence in fragments shorter than    3068 bp

# FokI.MfeI (GGATG+CAATTG) double digest, 5764 thousand fragments (5201116+564303 sites), 516 bp average fragment length
   5% of sequence in fragments shorter than     179 bp
  10% of sequence in fragments shorter than     275 bp
  15% of sequence in fragments shorter than     356 bp
  20% of sequence in fragments shorter than     437 bp
  25% of sequence in fragments shorter than     517 bp
  30% of sequence in fragments shorter than     596 bp
  35% of sequence in fragments shorter than     677 bp
  40% of sequence in fragments shorter than     759 bp
  45% of sequence in fragments shorter than     846 bp
  50% of sequence in fragments shorter than     934 bp
  55% of sequence in fragments shorter than    1033 bp
  60% of sequence in fragments shorter than    1136 bp
  65% of sequence in fragments shorter than    1250 bp
  70% of sequence in fragments shorter than    1380 bp
  75% of sequence in fragments shorter than    1529 bp
  80% of sequence in fragments shorter than    1715 bp
  85% of sequence in fragments shorter than    1946 bp
  90% of sequence in fragments shorter than    2279 bp
  95% of sequence in fragments shorter than    2857 bp

# BsrI.MluI (ACTGG+ACGCGT) double digest, 5762 thousand fragments (5741310+21415 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     430 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     661 bp
  40% of sequence in fragments shorter than     733 bp
  45% of sequence in fragments shorter than     819 bp
  50% of sequence in fragments shorter than     905 bp
  55% of sequence in fragments shorter than    1004 bp
  60% of sequence in fragments shorter than    1109 bp
  65% of sequence in fragments shorter than    1226 bp
  70% of sequence in fragments shorter than    1362 bp
  75% of sequence in fragments shorter than    1514 bp
  80% of sequence in fragments shorter than    1702 bp
  85% of sequence in fragments shorter than    1941 bp
  90% of sequence in fragments shorter than    2278 bp
  95% of sequence in fragments shorter than    2891 bp

# BglII.TfiI (AGATCT+GAWTC) double digest, 5758 thousand fragments (774732+4984362 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     177 bp
  10% of sequence in fragments shorter than     272 bp
  15% of sequence in fragments shorter than     345 bp
  20% of sequence in fragments shorter than     414 bp
  25% of sequence in fragments shorter than     492 bp
  30% of sequence in fragments shorter than     568 bp
  35% of sequence in fragments shorter than     645 bp
  40% of sequence in fragments shorter than     719 bp
  45% of sequence in fragments shorter than     801 bp
  50% of sequence in fragments shorter than     887 bp
  55% of sequence in fragments shorter than     980 bp
  60% of sequence in fragments shorter than    1079 bp
  65% of sequence in fragments shorter than    1191 bp
  70% of sequence in fragments shorter than    1318 bp
  75% of sequence in fragments shorter than    1463 bp
  80% of sequence in fragments shorter than    1638 bp
  85% of sequence in fragments shorter than    1859 bp
  90% of sequence in fragments shorter than    2162 bp
  95% of sequence in fragments shorter than    2678 bp

# AflII.CviQI (CTTAAG+GTAC) double digest, 5757 thousand fragments (643284+5115078 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     276 bp
  15% of sequence in fragments shorter than     359 bp
  20% of sequence in fragments shorter than     438 bp
  25% of sequence in fragments shorter than     511 bp
  30% of sequence in fragments shorter than     584 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     736 bp
  45% of sequence in fragments shorter than     819 bp
  50% of sequence in fragments shorter than     905 bp
  55% of sequence in fragments shorter than    1000 bp
  60% of sequence in fragments shorter than    1101 bp
  65% of sequence in fragments shorter than    1215 bp
  70% of sequence in fragments shorter than    1342 bp
  75% of sequence in fragments shorter than    1478 bp
  80% of sequence in fragments shorter than    1658 bp
  85% of sequence in fragments shorter than    1895 bp
  90% of sequence in fragments shorter than    2223 bp
  95% of sequence in fragments shorter than    2831 bp

# AciI.HgiCI (CCGC+GGYRCC) double digest, 5756 thousand fragments (4206130+1550877 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     161 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     432 bp
  20% of sequence in fragments shorter than     565 bp
  25% of sequence in fragments shorter than     699 bp
  30% of sequence in fragments shorter than     838 bp
  35% of sequence in fragments shorter than     978 bp
  40% of sequence in fragments shorter than    1126 bp
  45% of sequence in fragments shorter than    1284 bp
  50% of sequence in fragments shorter than    1461 bp
  55% of sequence in fragments shorter than    1658 bp
  60% of sequence in fragments shorter than    1883 bp
  65% of sequence in fragments shorter than    2133 bp
  70% of sequence in fragments shorter than    2424 bp
  75% of sequence in fragments shorter than    2766 bp
  80% of sequence in fragments shorter than    3187 bp
  85% of sequence in fragments shorter than    3738 bp
  90% of sequence in fragments shorter than    4517 bp
  95% of sequence in fragments shorter than    5879 bp

# BsrI.SgrAI (ACTGG+CRCCGGYG) double digest, 5756 thousand fragments (5741310+15270 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     430 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     734 bp
  45% of sequence in fragments shorter than     820 bp
  50% of sequence in fragments shorter than     907 bp
  55% of sequence in fragments shorter than    1005 bp
  60% of sequence in fragments shorter than    1110 bp
  65% of sequence in fragments shorter than    1228 bp
  70% of sequence in fragments shorter than    1364 bp
  75% of sequence in fragments shorter than    1516 bp
  80% of sequence in fragments shorter than    1705 bp
  85% of sequence in fragments shorter than    1944 bp
  90% of sequence in fragments shorter than    2282 bp
  95% of sequence in fragments shorter than    2896 bp

# BsrI.NruI (ACTGG+TCGCGA) double digest, 5755 thousand fragments (5741310+14872 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     430 bp
  25% of sequence in fragments shorter than     505 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     734 bp
  45% of sequence in fragments shorter than     820 bp
  50% of sequence in fragments shorter than     906 bp
  55% of sequence in fragments shorter than    1005 bp
  60% of sequence in fragments shorter than    1110 bp
  65% of sequence in fragments shorter than    1227 bp
  70% of sequence in fragments shorter than    1363 bp
  75% of sequence in fragments shorter than    1515 bp
  80% of sequence in fragments shorter than    1704 bp
  85% of sequence in fragments shorter than    1943 bp
  90% of sequence in fragments shorter than    2280 bp
  95% of sequence in fragments shorter than    2894 bp

# BsrI.PvuI (ACTGG+CGATCG) double digest, 5755 thousand fragments (5741310+14186 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     734 bp
  45% of sequence in fragments shorter than     820 bp
  50% of sequence in fragments shorter than     907 bp
  55% of sequence in fragments shorter than    1005 bp
  60% of sequence in fragments shorter than    1110 bp
  65% of sequence in fragments shorter than    1227 bp
  70% of sequence in fragments shorter than    1363 bp
  75% of sequence in fragments shorter than    1515 bp
  80% of sequence in fragments shorter than    1703 bp
  85% of sequence in fragments shorter than    1940 bp
  90% of sequence in fragments shorter than    2276 bp
  95% of sequence in fragments shorter than    2883 bp

# AciI.BsmI (CCGC+GAATGC) double digest, 5754 thousand fragments (4206130+1549350 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     162 bp
  10% of sequence in fragments shorter than     282 bp
  15% of sequence in fragments shorter than     403 bp
  20% of sequence in fragments shorter than     526 bp
  25% of sequence in fragments shorter than     649 bp
  30% of sequence in fragments shorter than     773 bp
  35% of sequence in fragments shorter than     896 bp
  40% of sequence in fragments shorter than    1033 bp
  45% of sequence in fragments shorter than    1172 bp
  50% of sequence in fragments shorter than    1322 bp
  55% of sequence in fragments shorter than    1485 bp
  60% of sequence in fragments shorter than    1666 bp
  65% of sequence in fragments shorter than    1871 bp
  70% of sequence in fragments shorter than    2104 bp
  75% of sequence in fragments shorter than    2378 bp
  80% of sequence in fragments shorter than    2707 bp
  85% of sequence in fragments shorter than    3125 bp
  90% of sequence in fragments shorter than    3705 bp
  95% of sequence in fragments shorter than    4710 bp

# BsrI.FseI (ACTGG+GGCCGGCC) double digest, 5754 thousand fragments (5741310+13662 sites), 517 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     734 bp
  45% of sequence in fragments shorter than     820 bp
  50% of sequence in fragments shorter than     907 bp
  55% of sequence in fragments shorter than    1006 bp
  60% of sequence in fragments shorter than    1110 bp
  65% of sequence in fragments shorter than    1228 bp
  70% of sequence in fragments shorter than    1364 bp
  75% of sequence in fragments shorter than    1516 bp
  80% of sequence in fragments shorter than    1705 bp
  85% of sequence in fragments shorter than    1945 bp
  90% of sequence in fragments shorter than    2282 bp
  95% of sequence in fragments shorter than    2896 bp

# FokI.SphI (GGATG+GCATGC) double digest, 5751 thousand fragments (5201116+550952 sites), 518 bp average fragment length
   5% of sequence in fragments shorter than     179 bp
  10% of sequence in fragments shorter than     274 bp
  15% of sequence in fragments shorter than     354 bp
  20% of sequence in fragments shorter than     436 bp
  25% of sequence in fragments shorter than     515 bp
  30% of sequence in fragments shorter than     595 bp
  35% of sequence in fragments shorter than     677 bp
  40% of sequence in fragments shorter than     761 bp
  45% of sequence in fragments shorter than     849 bp
  50% of sequence in fragments shorter than     939 bp
  55% of sequence in fragments shorter than    1039 bp
  60% of sequence in fragments shorter than    1147 bp
  65% of sequence in fragments shorter than    1264 bp
  70% of sequence in fragments shorter than    1393 bp
  75% of sequence in fragments shorter than    1549 bp
  80% of sequence in fragments shorter than    1739 bp
  85% of sequence in fragments shorter than    1987 bp
  90% of sequence in fragments shorter than    2338 bp
  95% of sequence in fragments shorter than    2970 bp

# BsrI.NotI (ACTGG+GCGGCCGC) double digest, 5750 thousand fragments (5741310+9795 sites), 518 bp average fragment length
   5% of sequence in fragments shorter than     177 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     350 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     584 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     735 bp
  45% of sequence in fragments shorter than     821 bp
  50% of sequence in fragments shorter than     907 bp
  55% of sequence in fragments shorter than    1006 bp
  60% of sequence in fragments shorter than    1111 bp
  65% of sequence in fragments shorter than    1228 bp
  70% of sequence in fragments shorter than    1364 bp
  75% of sequence in fragments shorter than    1517 bp
  80% of sequence in fragments shorter than    1706 bp
  85% of sequence in fragments shorter than    1945 bp
  90% of sequence in fragments shorter than    2283 bp
  95% of sequence in fragments shorter than    2898 bp

# BsrI.RsrII (ACTGG+CGGWCCG) double digest, 5750 thousand fragments (5741310+9764 sites), 518 bp average fragment length
   5% of sequence in fragments shorter than     177 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     350 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     584 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     735 bp
  45% of sequence in fragments shorter than     821 bp
  50% of sequence in fragments shorter than     907 bp
  55% of sequence in fragments shorter than    1006 bp
  60% of sequence in fragments shorter than    1111 bp
  65% of sequence in fragments shorter than    1229 bp
  70% of sequence in fragments shorter than    1365 bp
  75% of sequence in fragments shorter than    1517 bp
  80% of sequence in fragments shorter than    1706 bp
  85% of sequence in fragments shorter than    1946 bp
  90% of sequence in fragments shorter than    2283 bp
  95% of sequence in fragments shorter than    2898 bp

# ApaI.BbvI (GGGCCC+GCAGC) double digest, 5749 thousand fragments (460339+5290051 sites), 518 bp average fragment length
   5% of sequence in fragments shorter than     167 bp
  10% of sequence in fragments shorter than     273 bp
  15% of sequence in fragments shorter than     373 bp
  20% of sequence in fragments shorter than     475 bp
  25% of sequence in fragments shorter than     581 bp
  30% of sequence in fragments shorter than     688 bp
  35% of sequence in fragments shorter than     799 bp
  40% of sequence in fragments shorter than     908 bp
  45% of sequence in fragments shorter than    1031 bp
  50% of sequence in fragments shorter than    1163 bp
  55% of sequence in fragments shorter than    1304 bp
  60% of sequence in fragments shorter than    1462 bp
  65% of sequence in fragments shorter than    1628 bp
  70% of sequence in fragments shorter than    1825 bp
  75% of sequence in fragments shorter than    2059 bp
  80% of sequence in fragments shorter than    2335 bp
  85% of sequence in fragments shorter than    2707 bp
  90% of sequence in fragments shorter than    3214 bp
  95% of sequence in fragments shorter than    4112 bp

# AscI.BsrI (GGCGCGCC+ACTGG) double digest, 5745 thousand fragments (4751+5741310 sites), 518 bp average fragment length
   5% of sequence in fragments shorter than     177 bp
  10% of sequence in fragments shorter than     268 bp
  15% of sequence in fragments shorter than     350 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     584 bp
  35% of sequence in fragments shorter than     663 bp
  40% of sequence in fragments shorter than     735 bp
  45% of sequence in fragments shorter than     821 bp
  50% of sequence in fragments shorter than     908 bp
  55% of sequence in fragments shorter than    1007 bp
  60% of sequence in fragments shorter than    1111 bp
  65% of sequence in fragments shorter than    1230 bp
  70% of sequence in fragments shorter than    1366 bp
  75% of sequence in fragments shorter than    1518 bp
  80% of sequence in fragments shorter than    1707 bp
  85% of sequence in fragments shorter than    1947 bp
  90% of sequence in fragments shorter than    2284 bp
  95% of sequence in fragments shorter than    2900 bp

# CviQI.HaeII (GTAC+RGCGCY) double digest, 5744 thousand fragments (5115078+630101 sites), 518 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     277 bp
  15% of sequence in fragments shorter than     355 bp
  20% of sequence in fragments shorter than     434 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     582 bp
  35% of sequence in fragments shorter than     659 bp
  40% of sequence in fragments shorter than     733 bp
  45% of sequence in fragments shorter than     813 bp
  50% of sequence in fragments shorter than     898 bp
  55% of sequence in fragments shorter than     992 bp
  60% of sequence in fragments shorter than    1090 bp
  65% of sequence in fragments shorter than    1202 bp
  70% of sequence in fragments shorter than    1321 bp
  75% of sequence in fragments shorter than    1451 bp
  80% of sequence in fragments shorter than    1620 bp
  85% of sequence in fragments shorter than    1837 bp
  90% of sequence in fragments shorter than    2129 bp
  95% of sequence in fragments shorter than    2636 bp

# FokI.ScaI (GGATG+AGTACT) double digest, 5744 thousand fragments (5201116+543793 sites), 518 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     276 bp
  15% of sequence in fragments shorter than     356 bp
  20% of sequence in fragments shorter than     438 bp
  25% of sequence in fragments shorter than     517 bp
  30% of sequence in fragments shorter than     596 bp
  35% of sequence in fragments shorter than     678 bp
  40% of sequence in fragments shorter than     761 bp
  45% of sequence in fragments shorter than     848 bp
  50% of sequence in fragments shorter than     936 bp
  55% of sequence in fragments shorter than    1036 bp
  60% of sequence in fragments shorter than    1140 bp
  65% of sequence in fragments shorter than    1254 bp
  70% of sequence in fragments shorter than    1384 bp
  75% of sequence in fragments shorter than    1535 bp
  80% of sequence in fragments shorter than    1719 bp
  85% of sequence in fragments shorter than    1955 bp
  90% of sequence in fragments shorter than    2294 bp
  95% of sequence in fragments shorter than    2898 bp

# NcoI.TfiI (CCATGG+GAWTC) double digest, 5743 thousand fragments (759594+4984362 sites), 518 bp average fragment length
   5% of sequence in fragments shorter than     179 bp
  10% of sequence in fragments shorter than     273 bp
  15% of sequence in fragments shorter than     345 bp
  20% of sequence in fragments shorter than     414 bp
  25% of sequence in fragments shorter than     491 bp
  30% of sequence in fragments shorter than     566 bp
  35% of sequence in fragments shorter than     645 bp
  40% of sequence in fragments shorter than     720 bp
  45% of sequence in fragments shorter than     801 bp
  50% of sequence in fragments shorter than     886 bp
  55% of sequence in fragments shorter than     980 bp
  60% of sequence in fragments shorter than    1079 bp
  65% of sequence in fragments shorter than    1191 bp
  70% of sequence in fragments shorter than    1316 bp
  75% of sequence in fragments shorter than    1461 bp
  80% of sequence in fragments shorter than    1637 bp
  85% of sequence in fragments shorter than    1855 bp
  90% of sequence in fragments shorter than    2155 bp
  95% of sequence in fragments shorter than    2658 bp

# BbvI.EcoRV (GCAGC+GATATC) double digest, 5736 thousand fragments (5290051+446473 sites), 519 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     277 bp
  15% of sequence in fragments shorter than     371 bp
  20% of sequence in fragments shorter than     467 bp
  25% of sequence in fragments shorter than     562 bp
  30% of sequence in fragments shorter than     659 bp
  35% of sequence in fragments shorter than     758 bp
  40% of sequence in fragments shorter than     861 bp
  45% of sequence in fragments shorter than     967 bp
  50% of sequence in fragments shorter than    1083 bp
  55% of sequence in fragments shorter than    1210 bp
  60% of sequence in fragments shorter than    1345 bp
  65% of sequence in fragments shorter than    1499 bp
  70% of sequence in fragments shorter than    1659 bp
  75% of sequence in fragments shorter than    1862 bp
  80% of sequence in fragments shorter than    2099 bp
  85% of sequence in fragments shorter than    2410 bp
  90% of sequence in fragments shorter than    2845 bp
  95% of sequence in fragments shorter than    3593 bp

# BfiI.Tsp45I (ACTGGG+GTSAC) double digest, 5727 thousand fragments (1706594+4021761 sites), 520 bp average fragment length
   5% of sequence in fragments shorter than     181 bp
  10% of sequence in fragments shorter than     274 bp
  15% of sequence in fragments shorter than     350 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     663 bp
  40% of sequence in fragments shorter than     743 bp
  45% of sequence in fragments shorter than     830 bp
  50% of sequence in fragments shorter than     920 bp
  55% of sequence in fragments shorter than    1018 bp
  60% of sequence in fragments shorter than    1126 bp
  65% of sequence in fragments shorter than    1246 bp
  70% of sequence in fragments shorter than    1384 bp
  75% of sequence in fragments shorter than    1552 bp
  80% of sequence in fragments shorter than    1750 bp
  85% of sequence in fragments shorter than    2009 bp
  90% of sequence in fragments shorter than    2381 bp
  95% of sequence in fragments shorter than    2978 bp

# BclI.TfiI (TGATCA+GAWTC) double digest, 5722 thousand fragments (738785+4984362 sites), 520 bp average fragment length
   5% of sequence in fragments shorter than     179 bp
  10% of sequence in fragments shorter than     273 bp
  15% of sequence in fragments shorter than     345 bp
  20% of sequence in fragments shorter than     414 bp
  25% of sequence in fragments shorter than     492 bp
  30% of sequence in fragments shorter than     569 bp
  35% of sequence in fragments shorter than     645 bp
  40% of sequence in fragments shorter than     722 bp
  45% of sequence in fragments shorter than     802 bp
  50% of sequence in fragments shorter than     889 bp
  55% of sequence in fragments shorter than     984 bp
  60% of sequence in fragments shorter than    1084 bp
  65% of sequence in fragments shorter than    1197 bp
  70% of sequence in fragments shorter than    1324 bp
  75% of sequence in fragments shorter than    1470 bp
  80% of sequence in fragments shorter than    1647 bp
  85% of sequence in fragments shorter than    1869 bp
  90% of sequence in fragments shorter than    2175 bp
  95% of sequence in fragments shorter than    2699 bp

# CviQI.SacI (GTAC+GAGCTC) double digest, 5717 thousand fragments (5115078+603323 sites), 521 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     278 bp
  15% of sequence in fragments shorter than     361 bp
  20% of sequence in fragments shorter than     440 bp
  25% of sequence in fragments shorter than     513 bp
  30% of sequence in fragments shorter than     588 bp
  35% of sequence in fragments shorter than     665 bp
  40% of sequence in fragments shorter than     742 bp
  45% of sequence in fragments shorter than     821 bp
  50% of sequence in fragments shorter than     906 bp
  55% of sequence in fragments shorter than    1000 bp
  60% of sequence in fragments shorter than    1099 bp
  65% of sequence in fragments shorter than    1210 bp
  70% of sequence in fragments shorter than    1330 bp
  75% of sequence in fragments shorter than    1462 bp
  80% of sequence in fragments shorter than    1635 bp
  85% of sequence in fragments shorter than    1860 bp
  90% of sequence in fragments shorter than    2166 bp
  95% of sequence in fragments shorter than    2721 bp

# FnuDII.TfiI (CGCG+GAWTC) double digest, 5717 thousand fragments (733940+4984362 sites), 521 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     278 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     429 bp
  25% of sequence in fragments shorter than     511 bp
  30% of sequence in fragments shorter than     594 bp
  35% of sequence in fragments shorter than     675 bp
  40% of sequence in fragments shorter than     756 bp
  45% of sequence in fragments shorter than     840 bp
  50% of sequence in fragments shorter than     932 bp
  55% of sequence in fragments shorter than    1032 bp
  60% of sequence in fragments shorter than    1138 bp
  65% of sequence in fragments shorter than    1256 bp
  70% of sequence in fragments shorter than    1387 bp
  75% of sequence in fragments shorter than    1541 bp
  80% of sequence in fragments shorter than    1725 bp
  85% of sequence in fragments shorter than    1953 bp
  90% of sequence in fragments shorter than    2268 bp
  95% of sequence in fragments shorter than    2794 bp

# AvrII.CviQI (CCTAGG+GTAC) double digest, 5709 thousand fragments (594956+5115078 sites), 521 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     277 bp
  15% of sequence in fragments shorter than     361 bp
  20% of sequence in fragments shorter than     439 bp
  25% of sequence in fragments shorter than     514 bp
  30% of sequence in fragments shorter than     590 bp
  35% of sequence in fragments shorter than     667 bp
  40% of sequence in fragments shorter than     745 bp
  45% of sequence in fragments shorter than     827 bp
  50% of sequence in fragments shorter than     911 bp
  55% of sequence in fragments shorter than    1007 bp
  60% of sequence in fragments shorter than    1100 bp
  65% of sequence in fragments shorter than    1213 bp
  70% of sequence in fragments shorter than    1339 bp
  75% of sequence in fragments shorter than    1484 bp
  80% of sequence in fragments shorter than    1662 bp
  85% of sequence in fragments shorter than    1895 bp
  90% of sequence in fragments shorter than    2214 bp
  95% of sequence in fragments shorter than    2795 bp

# BsaAI.TfiI (YACGTR+GAWTC) double digest, 5697 thousand fragments (713801+4984362 sites), 523 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     276 bp
  15% of sequence in fragments shorter than     346 bp
  20% of sequence in fragments shorter than     419 bp
  25% of sequence in fragments shorter than     496 bp
  30% of sequence in fragments shorter than     574 bp
  35% of sequence in fragments shorter than     653 bp
  40% of sequence in fragments shorter than     729 bp
  45% of sequence in fragments shorter than     810 bp
  50% of sequence in fragments shorter than     898 bp
  55% of sequence in fragments shorter than     993 bp
  60% of sequence in fragments shorter than    1095 bp
  65% of sequence in fragments shorter than    1208 bp
  70% of sequence in fragments shorter than    1335 bp
  75% of sequence in fragments shorter than    1482 bp
  80% of sequence in fragments shorter than    1658 bp
  85% of sequence in fragments shorter than    1878 bp
  90% of sequence in fragments shorter than    2179 bp
  95% of sequence in fragments shorter than    2685 bp

# ApaLI.FokI (GTGCAC+GGATG) double digest, 5696 thousand fragments (496255+5201116 sites), 523 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     274 bp
  15% of sequence in fragments shorter than     356 bp
  20% of sequence in fragments shorter than     440 bp
  25% of sequence in fragments shorter than     521 bp
  30% of sequence in fragments shorter than     604 bp
  35% of sequence in fragments shorter than     688 bp
  40% of sequence in fragments shorter than     774 bp
  45% of sequence in fragments shorter than     865 bp
  50% of sequence in fragments shorter than     957 bp
  55% of sequence in fragments shorter than    1058 bp
  60% of sequence in fragments shorter than    1168 bp
  65% of sequence in fragments shorter than    1290 bp
  70% of sequence in fragments shorter than    1421 bp
  75% of sequence in fragments shorter than    1582 bp
  80% of sequence in fragments shorter than    1777 bp
  85% of sequence in fragments shorter than    2032 bp
  90% of sequence in fragments shorter than    2391 bp
  95% of sequence in fragments shorter than    3038 bp

# AciI.AflIII (CCGC+ACRYGT) double digest, 5691 thousand fragments (4206130+1485394 sites), 523 bp average fragment length
   5% of sequence in fragments shorter than     170 bp
  10% of sequence in fragments shorter than     299 bp
  15% of sequence in fragments shorter than     417 bp
  20% of sequence in fragments shorter than     534 bp
  25% of sequence in fragments shorter than     650 bp
  30% of sequence in fragments shorter than     766 bp
  35% of sequence in fragments shorter than     883 bp
  40% of sequence in fragments shorter than    1011 bp
  45% of sequence in fragments shorter than    1141 bp
  50% of sequence in fragments shorter than    1284 bp
  55% of sequence in fragments shorter than    1435 bp
  60% of sequence in fragments shorter than    1602 bp
  65% of sequence in fragments shorter than    1794 bp
  70% of sequence in fragments shorter than    2009 bp
  75% of sequence in fragments shorter than    2262 bp
  80% of sequence in fragments shorter than    2568 bp
  85% of sequence in fragments shorter than    2959 bp
  90% of sequence in fragments shorter than    3512 bp
  95% of sequence in fragments shorter than    4461 bp

# BbvI.SpeI (GCAGC+ACTAGT) double digest, 5689 thousand fragments (5290051+400286 sites), 523 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     277 bp
  15% of sequence in fragments shorter than     375 bp
  20% of sequence in fragments shorter than     473 bp
  25% of sequence in fragments shorter than     571 bp
  30% of sequence in fragments shorter than     670 bp
  35% of sequence in fragments shorter than     772 bp
  40% of sequence in fragments shorter than     873 bp
  45% of sequence in fragments shorter than     985 bp
  50% of sequence in fragments shorter than    1105 bp
  55% of sequence in fragments shorter than    1234 bp
  60% of sequence in fragments shorter than    1377 bp
  65% of sequence in fragments shorter than    1537 bp
  70% of sequence in fragments shorter than    1706 bp
  75% of sequence in fragments shorter than    1914 bp
  80% of sequence in fragments shorter than    2156 bp
  85% of sequence in fragments shorter than    2483 bp
  90% of sequence in fragments shorter than    2933 bp
  95% of sequence in fragments shorter than    3719 bp

# BceAI.TfiI (ACGGC+GAWTC) double digest, 5689 thousand fragments (705178+4984362 sites), 523 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     276 bp
  15% of sequence in fragments shorter than     346 bp
  20% of sequence in fragments shorter than     419 bp
  25% of sequence in fragments shorter than     496 bp
  30% of sequence in fragments shorter than     575 bp
  35% of sequence in fragments shorter than     654 bp
  40% of sequence in fragments shorter than     729 bp
  45% of sequence in fragments shorter than     809 bp
  50% of sequence in fragments shorter than     896 bp
  55% of sequence in fragments shorter than     990 bp
  60% of sequence in fragments shorter than    1090 bp
  65% of sequence in fragments shorter than    1203 bp
  70% of sequence in fragments shorter than    1328 bp
  75% of sequence in fragments shorter than    1473 bp
  80% of sequence in fragments shorter than    1649 bp
  85% of sequence in fragments shorter than    1868 bp
  90% of sequence in fragments shorter than    2168 bp
  95% of sequence in fragments shorter than    2671 bp

# HhaI.MlyI (GCGC+GAGTC) double digest, 5687 thousand fragments (1756502+3931692 sites), 523 bp average fragment length
   5% of sequence in fragments shorter than     170 bp
  10% of sequence in fragments shorter than     271 bp
  15% of sequence in fragments shorter than     358 bp
  20% of sequence in fragments shorter than     450 bp
  25% of sequence in fragments shorter than     539 bp
  30% of sequence in fragments shorter than     630 bp
  35% of sequence in fragments shorter than     715 bp
  40% of sequence in fragments shorter than     812 bp
  45% of sequence in fragments shorter than     913 bp
  50% of sequence in fragments shorter than    1022 bp
  55% of sequence in fragments shorter than    1139 bp
  60% of sequence in fragments shorter than    1271 bp
  65% of sequence in fragments shorter than    1417 bp
  70% of sequence in fragments shorter than    1587 bp
  75% of sequence in fragments shorter than    1780 bp
  80% of sequence in fragments shorter than    2022 bp
  85% of sequence in fragments shorter than    2341 bp
  90% of sequence in fragments shorter than    2786 bp
  95% of sequence in fragments shorter than    3573 bp

# CviQI.HgaI (GTAC+GACGC) double digest, 5686 thousand fragments (5115078+571889 sites), 524 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     279 bp
  15% of sequence in fragments shorter than     363 bp
  20% of sequence in fragments shorter than     442 bp
  25% of sequence in fragments shorter than     516 bp
  30% of sequence in fragments shorter than     592 bp
  35% of sequence in fragments shorter than     669 bp
  40% of sequence in fragments shorter than     748 bp
  45% of sequence in fragments shorter than     830 bp
  50% of sequence in fragments shorter than     917 bp
  55% of sequence in fragments shorter than    1012 bp
  60% of sequence in fragments shorter than    1112 bp
  65% of sequence in fragments shorter than    1221 bp
  70% of sequence in fragments shorter than    1344 bp
  75% of sequence in fragments shorter than    1479 bp
  80% of sequence in fragments shorter than    1655 bp
  85% of sequence in fragments shorter than    1883 bp
  90% of sequence in fragments shorter than    2195 bp
  95% of sequence in fragments shorter than    2760 bp

# CviQI.EciI (GTAC+GGCGGA) double digest, 5685 thousand fragments (5115078+571273 sites), 524 bp average fragment length
   5% of sequence in fragments shorter than     181 bp
  10% of sequence in fragments shorter than     277 bp
  15% of sequence in fragments shorter than     359 bp
  20% of sequence in fragments shorter than     438 bp
  25% of sequence in fragments shorter than     510 bp
  30% of sequence in fragments shorter than     585 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     738 bp
  45% of sequence in fragments shorter than     818 bp
  50% of sequence in fragments shorter than     904 bp
  55% of sequence in fragments shorter than     998 bp
  60% of sequence in fragments shorter than    1097 bp
  65% of sequence in fragments shorter than    1207 bp
  70% of sequence in fragments shorter than    1327 bp
  75% of sequence in fragments shorter than    1459 bp
  80% of sequence in fragments shorter than    1632 bp
  85% of sequence in fragments shorter than    1858 bp
  90% of sequence in fragments shorter than    2170 bp
  95% of sequence in fragments shorter than    2730 bp

# BciVI.TfiI (GTATCC+GAWTC) double digest, 5679 thousand fragments (696093+4984362 sites), 524 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     276 bp
  15% of sequence in fragments shorter than     346 bp
  20% of sequence in fragments shorter than     419 bp
  25% of sequence in fragments shorter than     497 bp
  30% of sequence in fragments shorter than     573 bp
  35% of sequence in fragments shorter than     653 bp
  40% of sequence in fragments shorter than     730 bp
  45% of sequence in fragments shorter than     811 bp
  50% of sequence in fragments shorter than     900 bp
  55% of sequence in fragments shorter than     996 bp
  60% of sequence in fragments shorter than    1098 bp
  65% of sequence in fragments shorter than    1211 bp
  70% of sequence in fragments shorter than    1340 bp
  75% of sequence in fragments shorter than    1488 bp
  80% of sequence in fragments shorter than    1666 bp
  85% of sequence in fragments shorter than    1888 bp
  90% of sequence in fragments shorter than    2195 bp
  95% of sequence in fragments shorter than    2714 bp

# BbvI.HpaI (GCAGC+GTTAAC) double digest, 5679 thousand fragments (5290051+389418 sites), 524 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     280 bp
  15% of sequence in fragments shorter than     375 bp
  20% of sequence in fragments shorter than     472 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     668 bp
  35% of sequence in fragments shorter than     770 bp
  40% of sequence in fragments shorter than     871 bp
  45% of sequence in fragments shorter than     982 bp
  50% of sequence in fragments shorter than    1100 bp
  55% of sequence in fragments shorter than    1226 bp
  60% of sequence in fragments shorter than    1369 bp
  65% of sequence in fragments shorter than    1528 bp
  70% of sequence in fragments shorter than    1697 bp
  75% of sequence in fragments shorter than    1905 bp
  80% of sequence in fragments shorter than    2152 bp
  85% of sequence in fragments shorter than    2480 bp
  90% of sequence in fragments shorter than    2931 bp
  95% of sequence in fragments shorter than    3727 bp

# CviQI.MfeI (GTAC+CAATTG) double digest, 5678 thousand fragments (5115078+564303 sites), 524 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     281 bp
  15% of sequence in fragments shorter than     364 bp
  20% of sequence in fragments shorter than     443 bp
  25% of sequence in fragments shorter than     518 bp
  30% of sequence in fragments shorter than     595 bp
  35% of sequence in fragments shorter than     673 bp
  40% of sequence in fragments shorter than     749 bp
  45% of sequence in fragments shorter than     832 bp
  50% of sequence in fragments shorter than     919 bp
  55% of sequence in fragments shorter than    1015 bp
  60% of sequence in fragments shorter than    1118 bp
  65% of sequence in fragments shorter than    1233 bp
  70% of sequence in fragments shorter than    1358 bp
  75% of sequence in fragments shorter than    1502 bp
  80% of sequence in fragments shorter than    1684 bp
  85% of sequence in fragments shorter than    1921 bp
  90% of sequence in fragments shorter than    2253 bp
  95% of sequence in fragments shorter than    2856 bp

# PsiI.Tsp45I (TTATAA+GTSAC) double digest, 5669 thousand fragments (1647912+4021761 sites), 525 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     278 bp
  15% of sequence in fragments shorter than     353 bp
  20% of sequence in fragments shorter than     429 bp
  25% of sequence in fragments shorter than     504 bp
  30% of sequence in fragments shorter than     577 bp
  35% of sequence in fragments shorter than     653 bp
  40% of sequence in fragments shorter than     729 bp
  45% of sequence in fragments shorter than     811 bp
  50% of sequence in fragments shorter than     894 bp
  55% of sequence in fragments shorter than     987 bp
  60% of sequence in fragments shorter than    1089 bp
  65% of sequence in fragments shorter than    1203 bp
  70% of sequence in fragments shorter than    1331 bp
  75% of sequence in fragments shorter than    1478 bp
  80% of sequence in fragments shorter than    1660 bp
  85% of sequence in fragments shorter than    1897 bp
  90% of sequence in fragments shorter than    2239 bp
  95% of sequence in fragments shorter than    2891 bp

# BbvI.SapI (GCAGC+GCTCTTC) double digest, 5666 thousand fragments (5290051+377161 sites), 525 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     280 bp
  15% of sequence in fragments shorter than     379 bp
  20% of sequence in fragments shorter than     478 bp
  25% of sequence in fragments shorter than     580 bp
  30% of sequence in fragments shorter than     683 bp
  35% of sequence in fragments shorter than     785 bp
  40% of sequence in fragments shorter than     892 bp
  45% of sequence in fragments shorter than    1010 bp
  50% of sequence in fragments shorter than    1136 bp
  55% of sequence in fragments shorter than    1271 bp
  60% of sequence in fragments shorter than    1412 bp
  65% of sequence in fragments shorter than    1581 bp
  70% of sequence in fragments shorter than    1761 bp
  75% of sequence in fragments shorter than    1983 bp
  80% of sequence in fragments shorter than    2251 bp
  85% of sequence in fragments shorter than    2606 bp
  90% of sequence in fragments shorter than    3090 bp
  95% of sequence in fragments shorter than    3951 bp

# BbvI.SmaI (GCAGC+CCCGGG) double digest, 5666 thousand fragments (5290051+376940 sites), 525 bp average fragment length
   5% of sequence in fragments shorter than     175 bp
  10% of sequence in fragments shorter than     281 bp
  15% of sequence in fragments shorter than     378 bp
  20% of sequence in fragments shorter than     478 bp
  25% of sequence in fragments shorter than     580 bp
  30% of sequence in fragments shorter than     682 bp
  35% of sequence in fragments shorter than     789 bp
  40% of sequence in fragments shorter than     895 bp
  45% of sequence in fragments shorter than    1014 bp
  50% of sequence in fragments shorter than    1141 bp
  55% of sequence in fragments shorter than    1278 bp
  60% of sequence in fragments shorter than    1430 bp
  65% of sequence in fragments shorter than    1602 bp
  70% of sequence in fragments shorter than    1781 bp
  75% of sequence in fragments shorter than    2010 bp
  80% of sequence in fragments shorter than    2274 bp
  85% of sequence in fragments shorter than    2638 bp
  90% of sequence in fragments shorter than    3129 bp
  95% of sequence in fragments shorter than    4008 bp

# CviQI.SphI (GTAC+GCATGC) double digest, 5665 thousand fragments (5115078+550952 sites), 525 bp average fragment length
   5% of sequence in fragments shorter than     184 bp
  10% of sequence in fragments shorter than     280 bp
  15% of sequence in fragments shorter than     364 bp
  20% of sequence in fragments shorter than     444 bp
  25% of sequence in fragments shorter than     517 bp
  30% of sequence in fragments shorter than     593 bp
  35% of sequence in fragments shorter than     670 bp
  40% of sequence in fragments shorter than     748 bp
  45% of sequence in fragments shorter than     830 bp
  50% of sequence in fragments shorter than     917 bp
  55% of sequence in fragments shorter than    1012 bp
  60% of sequence in fragments shorter than    1113 bp
  65% of sequence in fragments shorter than    1222 bp
  70% of sequence in fragments shorter than    1347 bp
  75% of sequence in fragments shorter than    1482 bp
  80% of sequence in fragments shorter than    1659 bp
  85% of sequence in fragments shorter than    1890 bp
  90% of sequence in fragments shorter than    2209 bp
  95% of sequence in fragments shorter than    2788 bp

# ApaI.FokI (GGGCCC+GGATG) double digest, 5660 thousand fragments (460339+5201116 sites), 526 bp average fragment length
   5% of sequence in fragments shorter than     178 bp
  10% of sequence in fragments shorter than     276 bp
  15% of sequence in fragments shorter than     361 bp
  20% of sequence in fragments shorter than     446 bp
  25% of sequence in fragments shorter than     530 bp
  30% of sequence in fragments shorter than     615 bp
  35% of sequence in fragments shorter than     702 bp
  40% of sequence in fragments shorter than     792 bp
  45% of sequence in fragments shorter than     887 bp
  50% of sequence in fragments shorter than     984 bp
  55% of sequence in fragments shorter than    1089 bp
  60% of sequence in fragments shorter than    1203 bp
  65% of sequence in fragments shorter than    1333 bp
  70% of sequence in fragments shorter than    1471 bp
  75% of sequence in fragments shorter than    1643 bp
  80% of sequence in fragments shorter than    1849 bp
  85% of sequence in fragments shorter than    2118 bp
  90% of sequence in fragments shorter than    2501 bp
  95% of sequence in fragments shorter than    3188 bp

# CviQI.ScaI (GTAC+AGTACT) double digest, 5658 thousand fragments (5115078+543793 sites), 526 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     312 bp
  15% of sequence in fragments shorter than     406 bp
  20% of sequence in fragments shorter than     492 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     662 bp
  35% of sequence in fragments shorter than     746 bp
  40% of sequence in fragments shorter than     833 bp
  45% of sequence in fragments shorter than     926 bp
  50% of sequence in fragments shorter than    1025 bp
  55% of sequence in fragments shorter than    1129 bp
  60% of sequence in fragments shorter than    1240 bp
  65% of sequence in fragments shorter than    1359 bp
  70% of sequence in fragments shorter than    1496 bp
  75% of sequence in fragments shorter than    1663 bp
  80% of sequence in fragments shorter than    1869 bp
  85% of sequence in fragments shorter than    2128 bp
  90% of sequence in fragments shorter than    2499 bp
  95% of sequence in fragments shorter than    3169 bp

# BamHI.BbvI (GGATCC+GCAGC) double digest, 5655 thousand fragments (365999+5290051 sites), 526 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     280 bp
  15% of sequence in fragments shorter than     378 bp
  20% of sequence in fragments shorter than     476 bp
  25% of sequence in fragments shorter than     578 bp
  30% of sequence in fragments shorter than     681 bp
  35% of sequence in fragments shorter than     788 bp
  40% of sequence in fragments shorter than     893 bp
  45% of sequence in fragments shorter than    1013 bp
  50% of sequence in fragments shorter than    1138 bp
  55% of sequence in fragments shorter than    1269 bp
  60% of sequence in fragments shorter than    1420 bp
  65% of sequence in fragments shorter than    1592 bp
  70% of sequence in fragments shorter than    1780 bp
  75% of sequence in fragments shorter than    2009 bp
  80% of sequence in fragments shorter than    2275 bp
  85% of sequence in fragments shorter than    2636 bp
  90% of sequence in fragments shorter than    3124 bp
  95% of sequence in fragments shorter than    3991 bp

# EcoRV.FokI (GATATC+GGATG) double digest, 5647 thousand fragments (446473+5201116 sites), 527 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     280 bp
  15% of sequence in fragments shorter than     362 bp
  20% of sequence in fragments shorter than     444 bp
  25% of sequence in fragments shorter than     524 bp
  30% of sequence in fragments shorter than     606 bp
  35% of sequence in fragments shorter than     689 bp
  40% of sequence in fragments shorter than     773 bp
  45% of sequence in fragments shorter than     862 bp
  50% of sequence in fragments shorter than     953 bp
  55% of sequence in fragments shorter than    1050 bp
  60% of sequence in fragments shorter than    1158 bp
  65% of sequence in fragments shorter than    1278 bp
  70% of sequence in fragments shorter than    1410 bp
  75% of sequence in fragments shorter than    1571 bp
  80% of sequence in fragments shorter than    1764 bp
  85% of sequence in fragments shorter than    2012 bp
  90% of sequence in fragments shorter than    2361 bp
  95% of sequence in fragments shorter than    2990 bp

# AciI.FauI (CCGC+CCCGC) double digest, 5645 thousand fragments (4206130+1439772 sites), 527 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     628 bp
  20% of sequence in fragments shorter than     826 bp
  25% of sequence in fragments shorter than    1022 bp
  30% of sequence in fragments shorter than    1227 bp
  35% of sequence in fragments shorter than    1444 bp
  40% of sequence in fragments shorter than    1680 bp
  45% of sequence in fragments shorter than    1938 bp
  50% of sequence in fragments shorter than    2224 bp
  55% of sequence in fragments shorter than    2538 bp
  60% of sequence in fragments shorter than    2888 bp
  65% of sequence in fragments shorter than    3289 bp
  70% of sequence in fragments shorter than    3757 bp
  75% of sequence in fragments shorter than    4309 bp
  80% of sequence in fragments shorter than    4987 bp
  85% of sequence in fragments shorter than    5863 bp
  90% of sequence in fragments shorter than    7098 bp
  95% of sequence in fragments shorter than    9264 bp

# AciI.BspMI (CCGC+ACCTGC) double digest, 5643 thousand fragments (4206130+1438129 sites), 528 bp average fragment length
   5% of sequence in fragments shorter than     164 bp
  10% of sequence in fragments shorter than     296 bp
  15% of sequence in fragments shorter than     416 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     669 bp
  30% of sequence in fragments shorter than     800 bp
  35% of sequence in fragments shorter than     929 bp
  40% of sequence in fragments shorter than    1071 bp
  45% of sequence in fragments shorter than    1227 bp
  50% of sequence in fragments shorter than    1390 bp
  55% of sequence in fragments shorter than    1575 bp
  60% of sequence in fragments shorter than    1784 bp
  65% of sequence in fragments shorter than    2013 bp
  70% of sequence in fragments shorter than    2285 bp
  75% of sequence in fragments shorter than    2603 bp
  80% of sequence in fragments shorter than    2985 bp
  85% of sequence in fragments shorter than    3475 bp
  90% of sequence in fragments shorter than    4185 bp
  95% of sequence in fragments shorter than    5379 bp

# BfiI.MlyI (ACTGGG+GAGTC) double digest, 5637 thousand fragments (1706594+3931692 sites), 528 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     277 bp
  15% of sequence in fragments shorter than     355 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     509 bp
  30% of sequence in fragments shorter than     587 bp
  35% of sequence in fragments shorter than     665 bp
  40% of sequence in fragments shorter than     747 bp
  45% of sequence in fragments shorter than     834 bp
  50% of sequence in fragments shorter than     927 bp
  55% of sequence in fragments shorter than    1028 bp
  60% of sequence in fragments shorter than    1138 bp
  65% of sequence in fragments shorter than    1265 bp
  70% of sequence in fragments shorter than    1405 bp
  75% of sequence in fragments shorter than    1567 bp
  80% of sequence in fragments shorter than    1770 bp
  85% of sequence in fragments shorter than    2039 bp
  90% of sequence in fragments shorter than    2412 bp
  95% of sequence in fragments shorter than    3107 bp

# AflII.TfiI (CTTAAG+GAWTC) double digest, 5627 thousand fragments (643284+4984362 sites), 529 bp average fragment length
   5% of sequence in fragments shorter than     181 bp
  10% of sequence in fragments shorter than     279 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     423 bp
  25% of sequence in fragments shorter than     501 bp
  30% of sequence in fragments shorter than     578 bp
  35% of sequence in fragments shorter than     658 bp
  40% of sequence in fragments shorter than     737 bp
  45% of sequence in fragments shorter than     819 bp
  50% of sequence in fragments shorter than     908 bp
  55% of sequence in fragments shorter than    1005 bp
  60% of sequence in fragments shorter than    1108 bp
  65% of sequence in fragments shorter than    1223 bp
  70% of sequence in fragments shorter than    1352 bp
  75% of sequence in fragments shorter than    1504 bp
  80% of sequence in fragments shorter than    1685 bp
  85% of sequence in fragments shorter than    1911 bp
  90% of sequence in fragments shorter than    2226 bp
  95% of sequence in fragments shorter than    2760 bp

# BbvI.BstEII (GCAGC+GGTNACC) double digest, 5625 thousand fragments (5290051+335865 sites), 529 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     281 bp
  15% of sequence in fragments shorter than     380 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     584 bp
  30% of sequence in fragments shorter than     687 bp
  35% of sequence in fragments shorter than     794 bp
  40% of sequence in fragments shorter than     900 bp
  45% of sequence in fragments shorter than    1020 bp
  50% of sequence in fragments shorter than    1148 bp
  55% of sequence in fragments shorter than    1285 bp
  60% of sequence in fragments shorter than    1438 bp
  65% of sequence in fragments shorter than    1609 bp
  70% of sequence in fragments shorter than    1788 bp
  75% of sequence in fragments shorter than    2016 bp
  80% of sequence in fragments shorter than    2278 bp
  85% of sequence in fragments shorter than    2636 bp
  90% of sequence in fragments shorter than    3127 bp
  95% of sequence in fragments shorter than    3995 bp

# EsaBC3I.Tsp45I (TCGA+GTSAC) double digest, 5624 thousand fragments (1603346+4021761 sites), 529 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     275 bp
  15% of sequence in fragments shorter than     353 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     506 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     662 bp
  40% of sequence in fragments shorter than     742 bp
  45% of sequence in fragments shorter than     829 bp
  50% of sequence in fragments shorter than     919 bp
  55% of sequence in fragments shorter than    1017 bp
  60% of sequence in fragments shorter than    1126 bp
  65% of sequence in fragments shorter than    1249 bp
  70% of sequence in fragments shorter than    1386 bp
  75% of sequence in fragments shorter than    1551 bp
  80% of sequence in fragments shorter than    1752 bp
  85% of sequence in fragments shorter than    2013 bp
  90% of sequence in fragments shorter than    2386 bp
  95% of sequence in fragments shorter than    3029 bp

# HaeII.TfiI (RGCGCY+GAWTC) double digest, 5613 thousand fragments (630101+4984362 sites), 530 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     278 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     428 bp
  25% of sequence in fragments shorter than     508 bp
  30% of sequence in fragments shorter than     588 bp
  35% of sequence in fragments shorter than     669 bp
  40% of sequence in fragments shorter than     748 bp
  45% of sequence in fragments shorter than     831 bp
  50% of sequence in fragments shorter than     922 bp
  55% of sequence in fragments shorter than    1020 bp
  60% of sequence in fragments shorter than    1124 bp
  65% of sequence in fragments shorter than    1240 bp
  70% of sequence in fragments shorter than    1370 bp
  75% of sequence in fragments shorter than    1520 bp
  80% of sequence in fragments shorter than    1701 bp
  85% of sequence in fragments shorter than    1926 bp
  90% of sequence in fragments shorter than    2235 bp
  95% of sequence in fragments shorter than    2752 bp

# ApaLI.CviQI (GTGCAC+GTAC) double digest, 5610 thousand fragments (496255+5115078 sites), 531 bp average fragment length
   5% of sequence in fragments shorter than     187 bp
  10% of sequence in fragments shorter than     285 bp
  15% of sequence in fragments shorter than     371 bp
  20% of sequence in fragments shorter than     452 bp
  25% of sequence in fragments shorter than     527 bp
  30% of sequence in fragments shorter than     605 bp
  35% of sequence in fragments shorter than     682 bp
  40% of sequence in fragments shorter than     761 bp
  45% of sequence in fragments shorter than     846 bp
  50% of sequence in fragments shorter than     935 bp
  55% of sequence in fragments shorter than    1031 bp
  60% of sequence in fragments shorter than    1134 bp
  65% of sequence in fragments shorter than    1245 bp
  70% of sequence in fragments shorter than    1362 bp
  75% of sequence in fragments shorter than    1509 bp
  80% of sequence in fragments shorter than    1689 bp
  85% of sequence in fragments shorter than    1922 bp
  90% of sequence in fragments shorter than    2244 bp
  95% of sequence in fragments shorter than    2828 bp

# FokI.SpeI (GGATG+ACTAGT) double digest, 5600 thousand fragments (5201116+400286 sites), 532 bp average fragment length
   5% of sequence in fragments shorter than     184 bp
  10% of sequence in fragments shorter than     282 bp
  15% of sequence in fragments shorter than     367 bp
  20% of sequence in fragments shorter than     451 bp
  25% of sequence in fragments shorter than     533 bp
  30% of sequence in fragments shorter than     615 bp
  35% of sequence in fragments shorter than     699 bp
  40% of sequence in fragments shorter than     786 bp
  45% of sequence in fragments shorter than     876 bp
  50% of sequence in fragments shorter than     967 bp
  55% of sequence in fragments shorter than    1067 bp
  60% of sequence in fragments shorter than    1175 bp
  65% of sequence in fragments shorter than    1297 bp
  70% of sequence in fragments shorter than    1425 bp
  75% of sequence in fragments shorter than    1581 bp
  80% of sequence in fragments shorter than    1776 bp
  85% of sequence in fragments shorter than    2023 bp
  90% of sequence in fragments shorter than    2373 bp
  95% of sequence in fragments shorter than    2991 bp

# FokI.HpaI (GGATG+GTTAAC) double digest, 5590 thousand fragments (5201116+389418 sites), 533 bp average fragment length
   5% of sequence in fragments shorter than     185 bp
  10% of sequence in fragments shorter than     283 bp
  15% of sequence in fragments shorter than     366 bp
  20% of sequence in fragments shorter than     450 bp
  25% of sequence in fragments shorter than     532 bp
  30% of sequence in fragments shorter than     614 bp
  35% of sequence in fragments shorter than     698 bp
  40% of sequence in fragments shorter than     784 bp
  45% of sequence in fragments shorter than     874 bp
  50% of sequence in fragments shorter than     966 bp
  55% of sequence in fragments shorter than    1066 bp
  60% of sequence in fragments shorter than    1172 bp
  65% of sequence in fragments shorter than    1294 bp
  70% of sequence in fragments shorter than    1425 bp
  75% of sequence in fragments shorter than    1585 bp
  80% of sequence in fragments shorter than    1777 bp
  85% of sequence in fragments shorter than    2026 bp
  90% of sequence in fragments shorter than    2380 bp
  95% of sequence in fragments shorter than    3020 bp

# BbvI.SexAI (GCAGC+ACCWGGT) double digest, 5587 thousand fragments (5290051+297900 sites), 533 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     283 bp
  15% of sequence in fragments shorter than     382 bp
  20% of sequence in fragments shorter than     483 bp
  25% of sequence in fragments shorter than     586 bp
  30% of sequence in fragments shorter than     689 bp
  35% of sequence in fragments shorter than     798 bp
  40% of sequence in fragments shorter than     904 bp
  45% of sequence in fragments shorter than    1025 bp
  50% of sequence in fragments shorter than    1153 bp
  55% of sequence in fragments shorter than    1290 bp
  60% of sequence in fragments shorter than    1442 bp
  65% of sequence in fragments shorter than    1614 bp
  70% of sequence in fragments shorter than    1795 bp
  75% of sequence in fragments shorter than    2023 bp
  80% of sequence in fragments shorter than    2288 bp
  85% of sequence in fragments shorter than    2648 bp
  90% of sequence in fragments shorter than    3137 bp
  95% of sequence in fragments shorter than    4012 bp

# SacI.TfiI (GAGCTC+GAWTC) double digest, 5587 thousand fragments (603323+4984362 sites), 533 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     282 bp
  15% of sequence in fragments shorter than     347 bp
  20% of sequence in fragments shorter than     426 bp
  25% of sequence in fragments shorter than     504 bp
  30% of sequence in fragments shorter than     583 bp
  35% of sequence in fragments shorter than     663 bp
  40% of sequence in fragments shorter than     740 bp
  45% of sequence in fragments shorter than     824 bp
  50% of sequence in fragments shorter than     914 bp
  55% of sequence in fragments shorter than    1010 bp
  60% of sequence in fragments shorter than    1113 bp
  65% of sequence in fragments shorter than    1228 bp
  70% of sequence in fragments shorter than    1357 bp
  75% of sequence in fragments shorter than    1507 bp
  80% of sequence in fragments shorter than    1687 bp
  85% of sequence in fragments shorter than    1911 bp
  90% of sequence in fragments shorter than    2220 bp
  95% of sequence in fragments shorter than    2738 bp

# MlyI.PsiI (GAGTC+TTATAA) double digest, 5579 thousand fragments (3931692+1647912 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     283 bp
  15% of sequence in fragments shorter than     359 bp
  20% of sequence in fragments shorter than     434 bp
  25% of sequence in fragments shorter than     508 bp
  30% of sequence in fragments shorter than     582 bp
  35% of sequence in fragments shorter than     656 bp
  40% of sequence in fragments shorter than     733 bp
  45% of sequence in fragments shorter than     813 bp
  50% of sequence in fragments shorter than     897 bp
  55% of sequence in fragments shorter than     990 bp
  60% of sequence in fragments shorter than    1091 bp
  65% of sequence in fragments shorter than    1203 bp
  70% of sequence in fragments shorter than    1331 bp
  75% of sequence in fragments shorter than    1480 bp
  80% of sequence in fragments shorter than    1666 bp
  85% of sequence in fragments shorter than    1894 bp
  90% of sequence in fragments shorter than    2243 bp
  95% of sequence in fragments shorter than    2919 bp

# AvrII.TfiI (CCTAGG+GAWTC) double digest, 5578 thousand fragments (594956+4984362 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     281 bp
  15% of sequence in fragments shorter than     348 bp
  20% of sequence in fragments shorter than     430 bp
  25% of sequence in fragments shorter than     509 bp
  30% of sequence in fragments shorter than     589 bp
  35% of sequence in fragments shorter than     668 bp
  40% of sequence in fragments shorter than     749 bp
  45% of sequence in fragments shorter than     831 bp
  50% of sequence in fragments shorter than     922 bp
  55% of sequence in fragments shorter than    1018 bp
  60% of sequence in fragments shorter than    1122 bp
  65% of sequence in fragments shorter than    1237 bp
  70% of sequence in fragments shorter than    1367 bp
  75% of sequence in fragments shorter than    1518 bp
  80% of sequence in fragments shorter than    1699 bp
  85% of sequence in fragments shorter than    1924 bp
  90% of sequence in fragments shorter than    2233 bp
  95% of sequence in fragments shorter than    2754 bp

# FokI.SapI (GGATG+GCTCTTC) double digest, 5577 thousand fragments (5201116+377161 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     282 bp
  15% of sequence in fragments shorter than     368 bp
  20% of sequence in fragments shorter than     453 bp
  25% of sequence in fragments shorter than     536 bp
  30% of sequence in fragments shorter than     619 bp
  35% of sequence in fragments shorter than     702 bp
  40% of sequence in fragments shorter than     790 bp
  45% of sequence in fragments shorter than     882 bp
  50% of sequence in fragments shorter than     977 bp
  55% of sequence in fragments shorter than    1079 bp
  60% of sequence in fragments shorter than    1193 bp
  65% of sequence in fragments shorter than    1316 bp
  70% of sequence in fragments shorter than    1451 bp
  75% of sequence in fragments shorter than    1620 bp
  80% of sequence in fragments shorter than    1819 bp
  85% of sequence in fragments shorter than    2077 bp
  90% of sequence in fragments shorter than    2443 bp
  95% of sequence in fragments shorter than    3102 bp

# FokI.SmaI (GGATG+CCCGGG) double digest, 5577 thousand fragments (5201116+376940 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     280 bp
  15% of sequence in fragments shorter than     365 bp
  20% of sequence in fragments shorter than     451 bp
  25% of sequence in fragments shorter than     535 bp
  30% of sequence in fragments shorter than     619 bp
  35% of sequence in fragments shorter than     705 bp
  40% of sequence in fragments shorter than     795 bp
  45% of sequence in fragments shorter than     889 bp
  50% of sequence in fragments shorter than     985 bp
  55% of sequence in fragments shorter than    1088 bp
  60% of sequence in fragments shorter than    1202 bp
  65% of sequence in fragments shorter than    1331 bp
  70% of sequence in fragments shorter than    1467 bp
  75% of sequence in fragments shorter than    1637 bp
  80% of sequence in fragments shorter than    1839 bp
  85% of sequence in fragments shorter than    2104 bp
  90% of sequence in fragments shorter than    2484 bp
  95% of sequence in fragments shorter than    3158 bp

# BbvI.KpnI (GCAGC+GGTACC) double digest, 5577 thousand fragments (5290051+287974 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     177 bp
  10% of sequence in fragments shorter than     284 bp
  15% of sequence in fragments shorter than     383 bp
  20% of sequence in fragments shorter than     485 bp
  25% of sequence in fragments shorter than     589 bp
  30% of sequence in fragments shorter than     689 bp
  35% of sequence in fragments shorter than     798 bp
  40% of sequence in fragments shorter than     903 bp
  45% of sequence in fragments shorter than    1022 bp
  50% of sequence in fragments shorter than    1143 bp
  55% of sequence in fragments shorter than    1278 bp
  60% of sequence in fragments shorter than    1431 bp
  65% of sequence in fragments shorter than    1594 bp
  70% of sequence in fragments shorter than    1786 bp
  75% of sequence in fragments shorter than    2011 bp
  80% of sequence in fragments shorter than    2287 bp
  85% of sequence in fragments shorter than    2648 bp
  90% of sequence in fragments shorter than    3136 bp
  95% of sequence in fragments shorter than    4024 bp

# BbvI.NheI (GCAGC+GCTAGC) double digest, 5576 thousand fragments (5290051+287384 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     178 bp
  10% of sequence in fragments shorter than     287 bp
  15% of sequence in fragments shorter than     387 bp
  20% of sequence in fragments shorter than     485 bp
  25% of sequence in fragments shorter than     587 bp
  30% of sequence in fragments shorter than     690 bp
  35% of sequence in fragments shorter than     797 bp
  40% of sequence in fragments shorter than     902 bp
  45% of sequence in fragments shorter than    1022 bp
  50% of sequence in fragments shorter than    1147 bp
  55% of sequence in fragments shorter than    1274 bp
  60% of sequence in fragments shorter than    1419 bp
  65% of sequence in fragments shorter than    1589 bp
  70% of sequence in fragments shorter than    1780 bp
  75% of sequence in fragments shorter than    2009 bp
  80% of sequence in fragments shorter than    2284 bp
  85% of sequence in fragments shorter than    2642 bp
  90% of sequence in fragments shorter than    3133 bp
  95% of sequence in fragments shorter than    4013 bp

# ApaI.CviQI (GGGCCC+GTAC) double digest, 5574 thousand fragments (460339+5115078 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     187 bp
  10% of sequence in fragments shorter than     285 bp
  15% of sequence in fragments shorter than     371 bp
  20% of sequence in fragments shorter than     452 bp
  25% of sequence in fragments shorter than     527 bp
  30% of sequence in fragments shorter than     604 bp
  35% of sequence in fragments shorter than     681 bp
  40% of sequence in fragments shorter than     761 bp
  45% of sequence in fragments shorter than     845 bp
  50% of sequence in fragments shorter than     933 bp
  55% of sequence in fragments shorter than    1029 bp
  60% of sequence in fragments shorter than    1132 bp
  65% of sequence in fragments shorter than    1241 bp
  70% of sequence in fragments shorter than    1360 bp
  75% of sequence in fragments shorter than    1504 bp
  80% of sequence in fragments shorter than    1682 bp
  85% of sequence in fragments shorter than    1912 bp
  90% of sequence in fragments shorter than    2231 bp
  95% of sequence in fragments shorter than    2807 bp

# HgiCI.Tsp45I (GGYRCC+GTSAC) double digest, 5572 thousand fragments (1550877+4021761 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     181 bp
  10% of sequence in fragments shorter than     277 bp
  15% of sequence in fragments shorter than     356 bp
  20% of sequence in fragments shorter than     437 bp
  25% of sequence in fragments shorter than     516 bp
  30% of sequence in fragments shorter than     596 bp
  35% of sequence in fragments shorter than     678 bp
  40% of sequence in fragments shorter than     763 bp
  45% of sequence in fragments shorter than     855 bp
  50% of sequence in fragments shorter than     951 bp
  55% of sequence in fragments shorter than    1055 bp
  60% of sequence in fragments shorter than    1169 bp
  65% of sequence in fragments shorter than    1290 bp
  70% of sequence in fragments shorter than    1438 bp
  75% of sequence in fragments shorter than    1609 bp
  80% of sequence in fragments shorter than    1815 bp
  85% of sequence in fragments shorter than    2085 bp
  90% of sequence in fragments shorter than    2469 bp
  95% of sequence in fragments shorter than    3158 bp

# BsmI.Tsp45I (GAATGC+GTSAC) double digest, 5570 thousand fragments (1549350+4021761 sites), 534 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     278 bp
  15% of sequence in fragments shorter than     361 bp
  20% of sequence in fragments shorter than     446 bp
  25% of sequence in fragments shorter than     529 bp
  30% of sequence in fragments shorter than     611 bp
  35% of sequence in fragments shorter than     695 bp
  40% of sequence in fragments shorter than     781 bp
  45% of sequence in fragments shorter than     870 bp
  50% of sequence in fragments shorter than     965 bp
  55% of sequence in fragments shorter than    1066 bp
  60% of sequence in fragments shorter than    1177 bp
  65% of sequence in fragments shorter than    1302 bp
  70% of sequence in fragments shorter than    1444 bp
  75% of sequence in fragments shorter than    1607 bp
  80% of sequence in fragments shorter than    1805 bp
  85% of sequence in fragments shorter than    2053 bp
  90% of sequence in fragments shorter than    2381 bp
  95% of sequence in fragments shorter than    2950 bp

# BamHI.FokI (GGATCC+GGATG) double digest, 5566 thousand fragments (365999+5201116 sites), 535 bp average fragment length
   5% of sequence in fragments shorter than     184 bp
  10% of sequence in fragments shorter than     283 bp
  15% of sequence in fragments shorter than     369 bp
  20% of sequence in fragments shorter than     452 bp
  25% of sequence in fragments shorter than     535 bp
  30% of sequence in fragments shorter than     615 bp
  35% of sequence in fragments shorter than     700 bp
  40% of sequence in fragments shorter than     790 bp
  45% of sequence in fragments shorter than     883 bp
  50% of sequence in fragments shorter than     982 bp
  55% of sequence in fragments shorter than    1087 bp
  60% of sequence in fragments shorter than    1200 bp
  65% of sequence in fragments shorter than    1328 bp
  70% of sequence in fragments shorter than    1464 bp
  75% of sequence in fragments shorter than    1633 bp
  80% of sequence in fragments shorter than    1834 bp
  85% of sequence in fragments shorter than    2096 bp
  90% of sequence in fragments shorter than    2470 bp
  95% of sequence in fragments shorter than    3145 bp

# CviQI.EcoRV (GTAC+GATATC) double digest, 5561 thousand fragments (5115078+446473 sites), 535 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     284 bp
  15% of sequence in fragments shorter than     370 bp
  20% of sequence in fragments shorter than     451 bp
  25% of sequence in fragments shorter than     527 bp
  30% of sequence in fragments shorter than     605 bp
  35% of sequence in fragments shorter than     684 bp
  40% of sequence in fragments shorter than     765 bp
  45% of sequence in fragments shorter than     851 bp
  50% of sequence in fragments shorter than     942 bp
  55% of sequence in fragments shorter than    1038 bp
  60% of sequence in fragments shorter than    1146 bp
  65% of sequence in fragments shorter than    1259 bp
  70% of sequence in fragments shorter than    1381 bp
  75% of sequence in fragments shorter than    1537 bp
  80% of sequence in fragments shorter than    1724 bp
  85% of sequence in fragments shorter than    1969 bp
  90% of sequence in fragments shorter than    2310 bp
  95% of sequence in fragments shorter than    2935 bp

# HgaI.TfiI (GACGC+GAWTC) double digest, 5555 thousand fragments (571889+4984362 sites), 536 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     284 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     430 bp
  25% of sequence in fragments shorter than     510 bp
  30% of sequence in fragments shorter than     592 bp
  35% of sequence in fragments shorter than     671 bp
  40% of sequence in fragments shorter than     750 bp
  45% of sequence in fragments shorter than     833 bp
  50% of sequence in fragments shorter than     924 bp
  55% of sequence in fragments shorter than    1021 bp
  60% of sequence in fragments shorter than    1126 bp
  65% of sequence in fragments shorter than    1241 bp
  70% of sequence in fragments shorter than    1370 bp
  75% of sequence in fragments shorter than    1521 bp
  80% of sequence in fragments shorter than    1701 bp
  85% of sequence in fragments shorter than    1925 bp
  90% of sequence in fragments shorter than    2233 bp
  95% of sequence in fragments shorter than    2749 bp

# EciI.TfiI (GGCGGA+GAWTC) double digest, 5555 thousand fragments (571273+4984362 sites), 536 bp average fragment length
   5% of sequence in fragments shorter than     179 bp
  10% of sequence in fragments shorter than     283 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     432 bp
  25% of sequence in fragments shorter than     513 bp
  30% of sequence in fragments shorter than     595 bp
  35% of sequence in fragments shorter than     675 bp
  40% of sequence in fragments shorter than     756 bp
  45% of sequence in fragments shorter than     839 bp
  50% of sequence in fragments shorter than     930 bp
  55% of sequence in fragments shorter than    1029 bp
  60% of sequence in fragments shorter than    1134 bp
  65% of sequence in fragments shorter than    1251 bp
  70% of sequence in fragments shorter than    1383 bp
  75% of sequence in fragments shorter than    1537 bp
  80% of sequence in fragments shorter than    1722 bp
  85% of sequence in fragments shorter than    1950 bp
  90% of sequence in fragments shorter than    2268 bp
  95% of sequence in fragments shorter than    2800 bp

# MfeI.TfiI (CAATTG+GAWTC) double digest, 5548 thousand fragments (564303+4984362 sites), 537 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     283 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     431 bp
  25% of sequence in fragments shorter than     511 bp
  30% of sequence in fragments shorter than     592 bp
  35% of sequence in fragments shorter than     671 bp
  40% of sequence in fragments shorter than     751 bp
  45% of sequence in fragments shorter than     835 bp
  50% of sequence in fragments shorter than     926 bp
  55% of sequence in fragments shorter than    1025 bp
  60% of sequence in fragments shorter than    1131 bp
  65% of sequence in fragments shorter than    1248 bp
  70% of sequence in fragments shorter than    1381 bp
  75% of sequence in fragments shorter than    1535 bp
  80% of sequence in fragments shorter than    1720 bp
  85% of sequence in fragments shorter than    1950 bp
  90% of sequence in fragments shorter than    2271 bp
  95% of sequence in fragments shorter than    2816 bp

# BstEII.FokI (GGTNACC+GGATG) double digest, 5536 thousand fragments (335865+5201116 sites), 538 bp average fragment length
   5% of sequence in fragments shorter than     185 bp
  10% of sequence in fragments shorter than     285 bp
  15% of sequence in fragments shorter than     371 bp
  20% of sequence in fragments shorter than     457 bp
  25% of sequence in fragments shorter than     540 bp
  30% of sequence in fragments shorter than     623 bp
  35% of sequence in fragments shorter than     708 bp
  40% of sequence in fragments shorter than     797 bp
  45% of sequence in fragments shorter than     891 bp
  50% of sequence in fragments shorter than     986 bp
  55% of sequence in fragments shorter than    1089 bp
  60% of sequence in fragments shorter than    1205 bp
  65% of sequence in fragments shorter than    1333 bp
  70% of sequence in fragments shorter than    1470 bp
  75% of sequence in fragments shorter than    1639 bp
  80% of sequence in fragments shorter than    1840 bp
  85% of sequence in fragments shorter than    2101 bp
  90% of sequence in fragments shorter than    2476 bp
  95% of sequence in fragments shorter than    3148 bp

# SphI.TfiI (GCATGC+GAWTC) double digest, 5534 thousand fragments (550952+4984362 sites), 538 bp average fragment length
   5% of sequence in fragments shorter than     185 bp
  10% of sequence in fragments shorter than     286 bp
  15% of sequence in fragments shorter than     351 bp
  20% of sequence in fragments shorter than     432 bp
  25% of sequence in fragments shorter than     512 bp
  30% of sequence in fragments shorter than     593 bp
  35% of sequence in fragments shorter than     672 bp
  40% of sequence in fragments shorter than     751 bp
  45% of sequence in fragments shorter than     834 bp
  50% of sequence in fragments shorter than     924 bp
  55% of sequence in fragments shorter than    1022 bp
  60% of sequence in fragments shorter than    1126 bp
  65% of sequence in fragments shorter than    1242 bp
  70% of sequence in fragments shorter than    1372 bp
  75% of sequence in fragments shorter than    1524 bp
  80% of sequence in fragments shorter than    1706 bp
  85% of sequence in fragments shorter than    1932 bp
  90% of sequence in fragments shorter than    2244 bp
  95% of sequence in fragments shorter than    2770 bp

# EsaBC3I.MlyI (TCGA+GAGTC) double digest, 5534 thousand fragments (1603346+3931692 sites), 538 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     275 bp
  15% of sequence in fragments shorter than     361 bp
  20% of sequence in fragments shorter than     442 bp
  25% of sequence in fragments shorter than     522 bp
  30% of sequence in fragments shorter than     603 bp
  35% of sequence in fragments shorter than     685 bp
  40% of sequence in fragments shorter than     770 bp
  45% of sequence in fragments shorter than     860 bp
  50% of sequence in fragments shorter than     955 bp
  55% of sequence in fragments shorter than    1059 bp
  60% of sequence in fragments shorter than    1174 bp
  65% of sequence in fragments shorter than    1302 bp
  70% of sequence in fragments shorter than    1443 bp
  75% of sequence in fragments shorter than    1615 bp
  80% of sequence in fragments shorter than    1828 bp
  85% of sequence in fragments shorter than    2105 bp
  90% of sequence in fragments shorter than    2505 bp
  95% of sequence in fragments shorter than    3192 bp

# ScaI.TfiI (AGTACT+GAWTC) double digest, 5527 thousand fragments (543793+4984362 sites), 539 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     284 bp
  15% of sequence in fragments shorter than     349 bp
  20% of sequence in fragments shorter than     430 bp
  25% of sequence in fragments shorter than     510 bp
  30% of sequence in fragments shorter than     591 bp
  35% of sequence in fragments shorter than     671 bp
  40% of sequence in fragments shorter than     750 bp
  45% of sequence in fragments shorter than     833 bp
  50% of sequence in fragments shorter than     924 bp
  55% of sequence in fragments shorter than    1022 bp
  60% of sequence in fragments shorter than    1125 bp
  65% of sequence in fragments shorter than    1242 bp
  70% of sequence in fragments shorter than    1374 bp
  75% of sequence in fragments shorter than    1527 bp
  80% of sequence in fragments shorter than    1711 bp
  85% of sequence in fragments shorter than    1941 bp
  90% of sequence in fragments shorter than    2259 bp
  95% of sequence in fragments shorter than    2801 bp

# AciI.PstI (CCGC+CTGCAG) double digest, 5527 thousand fragments (4206130+1321469 sites), 539 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     304 bp
  15% of sequence in fragments shorter than     432 bp
  20% of sequence in fragments shorter than     560 bp
  25% of sequence in fragments shorter than     689 bp
  30% of sequence in fragments shorter than     825 bp
  35% of sequence in fragments shorter than     963 bp
  40% of sequence in fragments shorter than    1109 bp
  45% of sequence in fragments shorter than    1274 bp
  50% of sequence in fragments shorter than    1451 bp
  55% of sequence in fragments shorter than    1649 bp
  60% of sequence in fragments shorter than    1871 bp
  65% of sequence in fragments shorter than    2121 bp
  70% of sequence in fragments shorter than    2414 bp
  75% of sequence in fragments shorter than    2756 bp
  80% of sequence in fragments shorter than    3168 bp
  85% of sequence in fragments shorter than    3722 bp
  90% of sequence in fragments shorter than    4489 bp
  95% of sequence in fragments shorter than    5833 bp

# BbvI.BsrBI (GCAGC+CCGCTC) double digest, 5525 thousand fragments (5290051+235523 sites), 539 bp average fragment length
   5% of sequence in fragments shorter than     177 bp
  10% of sequence in fragments shorter than     288 bp
  15% of sequence in fragments shorter than     391 bp
  20% of sequence in fragments shorter than     496 bp
  25% of sequence in fragments shorter than     603 bp
  30% of sequence in fragments shorter than     710 bp
  35% of sequence in fragments shorter than     823 bp
  40% of sequence in fragments shorter than     933 bp
  45% of sequence in fragments shorter than    1054 bp
  50% of sequence in fragments shorter than    1188 bp
  55% of sequence in fragments shorter than    1330 bp
  60% of sequence in fragments shorter than    1487 bp
  65% of sequence in fragments shorter than    1655 bp
  70% of sequence in fragments shorter than    1858 bp
  75% of sequence in fragments shorter than    2082 bp
  80% of sequence in fragments shorter than    2373 bp
  85% of sequence in fragments shorter than    2744 bp
  90% of sequence in fragments shorter than    3260 bp
  95% of sequence in fragments shorter than    4154 bp

# BbvI.KasI (GCAGC+GGCGCC) double digest, 5519 thousand fragments (5290051+230381 sites), 539 bp average fragment length
   5% of sequence in fragments shorter than     179 bp
  10% of sequence in fragments shorter than     288 bp
  15% of sequence in fragments shorter than     389 bp
  20% of sequence in fragments shorter than     492 bp
  25% of sequence in fragments shorter than     596 bp
  30% of sequence in fragments shorter than     700 bp
  35% of sequence in fragments shorter than     812 bp
  40% of sequence in fragments shorter than     920 bp
  45% of sequence in fragments shorter than    1040 bp
  50% of sequence in fragments shorter than    1173 bp
  55% of sequence in fragments shorter than    1313 bp
  60% of sequence in fragments shorter than    1468 bp
  65% of sequence in fragments shorter than    1632 bp
  70% of sequence in fragments shorter than    1828 bp
  75% of sequence in fragments shorter than    2060 bp
  80% of sequence in fragments shorter than    2332 bp
  85% of sequence in fragments shorter than    2700 bp
  90% of sequence in fragments shorter than    3202 bp
  95% of sequence in fragments shorter than    4094 bp

# BbvI.SwaI (GCAGC+ATTTAAAT) double digest, 5516 thousand fragments (5290051+226602 sites), 540 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     290 bp
  15% of sequence in fragments shorter than     389 bp
  20% of sequence in fragments shorter than     490 bp
  25% of sequence in fragments shorter than     592 bp
  30% of sequence in fragments shorter than     692 bp
  35% of sequence in fragments shorter than     800 bp
  40% of sequence in fragments shorter than     904 bp
  45% of sequence in fragments shorter than    1022 bp
  50% of sequence in fragments shorter than    1146 bp
  55% of sequence in fragments shorter than    1278 bp
  60% of sequence in fragments shorter than    1426 bp
  65% of sequence in fragments shorter than    1592 bp
  70% of sequence in fragments shorter than    1767 bp
  75% of sequence in fragments shorter than    1982 bp
  80% of sequence in fragments shorter than    2235 bp
  85% of sequence in fragments shorter than    2579 bp
  90% of sequence in fragments shorter than    3045 bp
  95% of sequence in fragments shorter than    3867 bp

# CviQI.SpeI (GTAC+ACTAGT) double digest, 5514 thousand fragments (5115078+400286 sites), 540 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     290 bp
  15% of sequence in fragments shorter than     378 bp
  20% of sequence in fragments shorter than     461 bp
  25% of sequence in fragments shorter than     537 bp
  30% of sequence in fragments shorter than     613 bp
  35% of sequence in fragments shorter than     691 bp
  40% of sequence in fragments shorter than     773 bp
  45% of sequence in fragments shorter than     855 bp
  50% of sequence in fragments shorter than     947 bp
  55% of sequence in fragments shorter than    1044 bp
  60% of sequence in fragments shorter than    1154 bp
  65% of sequence in fragments shorter than    1268 bp
  70% of sequence in fragments shorter than    1398 bp
  75% of sequence in fragments shorter than    1557 bp
  80% of sequence in fragments shorter than    1747 bp
  85% of sequence in fragments shorter than    1997 bp
  90% of sequence in fragments shorter than    2345 bp
  95% of sequence in fragments shorter than    2988 bp

# AflIII.Tsp45I (ACRYGT+GTSAC) double digest, 5506 thousand fragments (1485394+4021761 sites), 541 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     293 bp
  15% of sequence in fragments shorter than     373 bp
  20% of sequence in fragments shorter than     451 bp
  25% of sequence in fragments shorter than     532 bp
  30% of sequence in fragments shorter than     611 bp
  35% of sequence in fragments shorter than     691 bp
  40% of sequence in fragments shorter than     774 bp
  45% of sequence in fragments shorter than     862 bp
  50% of sequence in fragments shorter than     954 bp
  55% of sequence in fragments shorter than    1052 bp
  60% of sequence in fragments shorter than    1162 bp
  65% of sequence in fragments shorter than    1284 bp
  70% of sequence in fragments shorter than    1421 bp
  75% of sequence in fragments shorter than    1584 bp
  80% of sequence in fragments shorter than    1782 bp
  85% of sequence in fragments shorter than    2039 bp
  90% of sequence in fragments shorter than    2409 bp
  95% of sequence in fragments shorter than    3082 bp

# CviQI.HpaI (GTAC+GTTAAC) double digest, 5504 thousand fragments (5115078+389418 sites), 541 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     289 bp
  15% of sequence in fragments shorter than     375 bp
  20% of sequence in fragments shorter than     458 bp
  25% of sequence in fragments shorter than     535 bp
  30% of sequence in fragments shorter than     612 bp
  35% of sequence in fragments shorter than     691 bp
  40% of sequence in fragments shorter than     773 bp
  45% of sequence in fragments shorter than     860 bp
  50% of sequence in fragments shorter than     952 bp
  55% of sequence in fragments shorter than    1049 bp
  60% of sequence in fragments shorter than    1159 bp
  65% of sequence in fragments shorter than    1272 bp
  70% of sequence in fragments shorter than    1393 bp
  75% of sequence in fragments shorter than    1550 bp
  80% of sequence in fragments shorter than    1739 bp
  85% of sequence in fragments shorter than    1986 bp
  90% of sequence in fragments shorter than    2331 bp
  95% of sequence in fragments shorter than    2970 bp

# FokI.SexAI (GGATG+ACCWGGT) double digest, 5498 thousand fragments (5201116+297900 sites), 541 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     286 bp
  15% of sequence in fragments shorter than     373 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     543 bp
  30% of sequence in fragments shorter than     628 bp
  35% of sequence in fragments shorter than     714 bp
  40% of sequence in fragments shorter than     803 bp
  45% of sequence in fragments shorter than     897 bp
  50% of sequence in fragments shorter than     992 bp
  55% of sequence in fragments shorter than    1096 bp
  60% of sequence in fragments shorter than    1208 bp
  65% of sequence in fragments shorter than    1336 bp
  70% of sequence in fragments shorter than    1472 bp
  75% of sequence in fragments shorter than    1640 bp
  80% of sequence in fragments shorter than    1841 bp
  85% of sequence in fragments shorter than    2103 bp
  90% of sequence in fragments shorter than    2477 bp
  95% of sequence in fragments shorter than    3134 bp

# CviQI.SapI (GTAC+GCTCTTC) double digest, 5491 thousand fragments (5115078+377161 sites), 542 bp average fragment length
   5% of sequence in fragments shorter than     189 bp
  10% of sequence in fragments shorter than     290 bp
  15% of sequence in fragments shorter than     376 bp
  20% of sequence in fragments shorter than     457 bp
  25% of sequence in fragments shorter than     534 bp
  30% of sequence in fragments shorter than     612 bp
  35% of sequence in fragments shorter than     690 bp
  40% of sequence in fragments shorter than     771 bp
  45% of sequence in fragments shorter than     857 bp
  50% of sequence in fragments shorter than     949 bp
  55% of sequence in fragments shorter than    1045 bp
  60% of sequence in fragments shorter than    1152 bp
  65% of sequence in fragments shorter than    1265 bp
  70% of sequence in fragments shorter than    1382 bp
  75% of sequence in fragments shorter than    1536 bp
  80% of sequence in fragments shorter than    1721 bp
  85% of sequence in fragments shorter than    1961 bp
  90% of sequence in fragments shorter than    2292 bp
  95% of sequence in fragments shorter than    2895 bp

# CviQI.SmaI (GTAC+CCCGGG) double digest, 5491 thousand fragments (5115078+376940 sites), 542 bp average fragment length
   5% of sequence in fragments shorter than     192 bp
  10% of sequence in fragments shorter than     290 bp
  15% of sequence in fragments shorter than     375 bp
  20% of sequence in fragments shorter than     457 bp
  25% of sequence in fragments shorter than     532 bp
  30% of sequence in fragments shorter than     609 bp
  35% of sequence in fragments shorter than     686 bp
  40% of sequence in fragments shorter than     766 bp
  45% of sequence in fragments shorter than     851 bp
  50% of sequence in fragments shorter than     940 bp
  55% of sequence in fragments shorter than    1034 bp
  60% of sequence in fragments shorter than    1139 bp
  65% of sequence in fragments shorter than    1248 bp
  70% of sequence in fragments shorter than    1366 bp
  75% of sequence in fragments shorter than    1513 bp
  80% of sequence in fragments shorter than    1692 bp
  85% of sequence in fragments shorter than    1923 bp
  90% of sequence in fragments shorter than    2242 bp
  95% of sequence in fragments shorter than    2821 bp

# SspI.StyI (AATATT+CCWWGG) double digest, 5490 thousand fragments (2377267+3114107 sites), 542 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     287 bp
  15% of sequence in fragments shorter than     370 bp
  20% of sequence in fragments shorter than     448 bp
  25% of sequence in fragments shorter than     521 bp
  30% of sequence in fragments shorter than     596 bp
  35% of sequence in fragments shorter than     673 bp
  40% of sequence in fragments shorter than     750 bp
  45% of sequence in fragments shorter than     830 bp
  50% of sequence in fragments shorter than     919 bp
  55% of sequence in fragments shorter than    1014 bp
  60% of sequence in fragments shorter than    1116 bp
  65% of sequence in fragments shorter than    1230 bp
  70% of sequence in fragments shorter than    1359 bp
  75% of sequence in fragments shorter than    1505 bp
  80% of sequence in fragments shorter than    1687 bp
  85% of sequence in fragments shorter than    1913 bp
  90% of sequence in fragments shorter than    2246 bp
  95% of sequence in fragments shorter than    2811 bp

# FokI.KpnI (GGATG+GGTACC) double digest, 5488 thousand fragments (5201116+287974 sites), 542 bp average fragment length
   5% of sequence in fragments shorter than     187 bp
  10% of sequence in fragments shorter than     285 bp
  15% of sequence in fragments shorter than     372 bp
  20% of sequence in fragments shorter than     460 bp
  25% of sequence in fragments shorter than     545 bp
  30% of sequence in fragments shorter than     629 bp
  35% of sequence in fragments shorter than     715 bp
  40% of sequence in fragments shorter than     800 bp
  45% of sequence in fragments shorter than     893 bp
  50% of sequence in fragments shorter than     992 bp
  55% of sequence in fragments shorter than    1095 bp
  60% of sequence in fragments shorter than    1210 bp
  65% of sequence in fragments shorter than    1335 bp
  70% of sequence in fragments shorter than    1477 bp
  75% of sequence in fragments shorter than    1650 bp
  80% of sequence in fragments shorter than    1853 bp
  85% of sequence in fragments shorter than    2120 bp
  90% of sequence in fragments shorter than    2497 bp
  95% of sequence in fragments shorter than    3176 bp

# FokI.NheI (GGATG+GCTAGC) double digest, 5488 thousand fragments (5201116+287384 sites), 543 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     288 bp
  15% of sequence in fragments shorter than     373 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     542 bp
  30% of sequence in fragments shorter than     620 bp
  35% of sequence in fragments shorter than     706 bp
  40% of sequence in fragments shorter than     794 bp
  45% of sequence in fragments shorter than     884 bp
  50% of sequence in fragments shorter than     982 bp
  55% of sequence in fragments shorter than    1086 bp
  60% of sequence in fragments shorter than    1199 bp
  65% of sequence in fragments shorter than    1328 bp
  70% of sequence in fragments shorter than    1469 bp
  75% of sequence in fragments shorter than    1640 bp
  80% of sequence in fragments shorter than    1844 bp
  85% of sequence in fragments shorter than    2109 bp
  90% of sequence in fragments shorter than    2485 bp
  95% of sequence in fragments shorter than    3161 bp

# HgiCI.MlyI (GGYRCC+GAGTC) double digest, 5482 thousand fragments (1550877+3931692 sites), 543 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     276 bp
  15% of sequence in fragments shorter than     362 bp
  20% of sequence in fragments shorter than     446 bp
  25% of sequence in fragments shorter than     529 bp
  30% of sequence in fragments shorter than     615 bp
  35% of sequence in fragments shorter than     701 bp
  40% of sequence in fragments shorter than     791 bp
  45% of sequence in fragments shorter than     886 bp
  50% of sequence in fragments shorter than     990 bp
  55% of sequence in fragments shorter than    1099 bp
  60% of sequence in fragments shorter than    1220 bp
  65% of sequence in fragments shorter than    1352 bp
  70% of sequence in fragments shorter than    1506 bp
  75% of sequence in fragments shorter than    1692 bp
  80% of sequence in fragments shorter than    1920 bp
  85% of sequence in fragments shorter than    2224 bp
  90% of sequence in fragments shorter than    2668 bp
  95% of sequence in fragments shorter than    3508 bp

# NspI.SspI (RCATGY+AATATT) double digest, 5481 thousand fragments (3104359+2377267 sites), 543 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     282 bp
  15% of sequence in fragments shorter than     370 bp
  20% of sequence in fragments shorter than     451 bp
  25% of sequence in fragments shorter than     534 bp
  30% of sequence in fragments shorter than     618 bp
  35% of sequence in fragments shorter than     702 bp
  40% of sequence in fragments shorter than     790 bp
  45% of sequence in fragments shorter than     884 bp
  50% of sequence in fragments shorter than     986 bp
  55% of sequence in fragments shorter than    1093 bp
  60% of sequence in fragments shorter than    1210 bp
  65% of sequence in fragments shorter than    1347 bp
  70% of sequence in fragments shorter than    1499 bp
  75% of sequence in fragments shorter than    1679 bp
  80% of sequence in fragments shorter than    1902 bp
  85% of sequence in fragments shorter than    2197 bp
  90% of sequence in fragments shorter than    2629 bp
  95% of sequence in fragments shorter than    3427 bp

# BamHI.CviQI (GGATCC+GTAC) double digest, 5480 thousand fragments (365999+5115078 sites), 543 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     289 bp
  15% of sequence in fragments shorter than     377 bp
  20% of sequence in fragments shorter than     460 bp
  25% of sequence in fragments shorter than     537 bp
  30% of sequence in fragments shorter than     615 bp
  35% of sequence in fragments shorter than     693 bp
  40% of sequence in fragments shorter than     775 bp
  45% of sequence in fragments shorter than     861 bp
  50% of sequence in fragments shorter than     951 bp
  55% of sequence in fragments shorter than    1046 bp
  60% of sequence in fragments shorter than    1154 bp
  65% of sequence in fragments shorter than    1258 bp
  70% of sequence in fragments shorter than    1385 bp
  75% of sequence in fragments shorter than    1538 bp
  80% of sequence in fragments shorter than    1722 bp
  85% of sequence in fragments shorter than    1959 bp
  90% of sequence in fragments shorter than    2282 bp
  95% of sequence in fragments shorter than    2870 bp

# BsmI.MlyI (GAATGC+GAGTC) double digest, 5480 thousand fragments (1549350+3931692 sites), 543 bp average fragment length
   5% of sequence in fragments shorter than     176 bp
  10% of sequence in fragments shorter than     283 bp
  15% of sequence in fragments shorter than     364 bp
  20% of sequence in fragments shorter than     448 bp
  25% of sequence in fragments shorter than     528 bp
  30% of sequence in fragments shorter than     607 bp
  35% of sequence in fragments shorter than     690 bp
  40% of sequence in fragments shorter than     775 bp
  45% of sequence in fragments shorter than     864 bp
  50% of sequence in fragments shorter than     957 bp
  55% of sequence in fragments shorter than    1055 bp
  60% of sequence in fragments shorter than    1165 bp
  65% of sequence in fragments shorter than    1289 bp
  70% of sequence in fragments shorter than    1428 bp
  75% of sequence in fragments shorter than    1587 bp
  80% of sequence in fragments shorter than    1784 bp
  85% of sequence in fragments shorter than    2027 bp
  90% of sequence in fragments shorter than    2367 bp
  95% of sequence in fragments shorter than    2946 bp

# ApaLI.TfiI (GTGCAC+GAWTC) double digest, 5480 thousand fragments (496255+4984362 sites), 543 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     289 bp
  15% of sequence in fragments shorter than     355 bp
  20% of sequence in fragments shorter than     437 bp
  25% of sequence in fragments shorter than     517 bp
  30% of sequence in fragments shorter than     595 bp
  35% of sequence in fragments shorter than     675 bp
  40% of sequence in fragments shorter than     755 bp
  45% of sequence in fragments shorter than     838 bp
  50% of sequence in fragments shorter than     928 bp
  55% of sequence in fragments shorter than    1026 bp
  60% of sequence in fragments shorter than    1131 bp
  65% of sequence in fragments shorter than    1247 bp
  70% of sequence in fragments shorter than    1378 bp
  75% of sequence in fragments shorter than    1530 bp
  80% of sequence in fragments shorter than    1713 bp
  85% of sequence in fragments shorter than    1940 bp
  90% of sequence in fragments shorter than    2255 bp
  95% of sequence in fragments shorter than    2783 bp

# AvaII.SmlI (GGWCC+CTYRAG) double digest, 5475 thousand fragments (2747690+2727867 sites), 544 bp average fragment length
   5% of sequence in fragments shorter than     178 bp
  10% of sequence in fragments shorter than     281 bp
  15% of sequence in fragments shorter than     366 bp
  20% of sequence in fragments shorter than     452 bp
  25% of sequence in fragments shorter than     536 bp
  30% of sequence in fragments shorter than     623 bp
  35% of sequence in fragments shorter than     708 bp
  40% of sequence in fragments shorter than     796 bp
  45% of sequence in fragments shorter than     892 bp
  50% of sequence in fragments shorter than     991 bp
  55% of sequence in fragments shorter than    1101 bp
  60% of sequence in fragments shorter than    1221 bp
  65% of sequence in fragments shorter than    1359 bp
  70% of sequence in fragments shorter than    1513 bp
  75% of sequence in fragments shorter than    1698 bp
  80% of sequence in fragments shorter than    1910 bp
  85% of sequence in fragments shorter than    2190 bp
  90% of sequence in fragments shorter than    2593 bp
  95% of sequence in fragments shorter than    3310 bp

# AclI.BbvI (AACGTT+GCAGC) double digest, 5462 thousand fragments (172706+5290051 sites), 545 bp average fragment length
   5% of sequence in fragments shorter than     181 bp
  10% of sequence in fragments shorter than     292 bp
  15% of sequence in fragments shorter than     392 bp
  20% of sequence in fragments shorter than     496 bp
  25% of sequence in fragments shorter than     601 bp
  30% of sequence in fragments shorter than     705 bp
  35% of sequence in fragments shorter than     816 bp
  40% of sequence in fragments shorter than     924 bp
  45% of sequence in fragments shorter than    1042 bp
  50% of sequence in fragments shorter than    1173 bp
  55% of sequence in fragments shorter than    1309 bp
  60% of sequence in fragments shorter than    1462 bp
  65% of sequence in fragments shorter than    1628 bp
  70% of sequence in fragments shorter than    1817 bp
  75% of sequence in fragments shorter than    2045 bp
  80% of sequence in fragments shorter than    2309 bp
  85% of sequence in fragments shorter than    2667 bp
  90% of sequence in fragments shorter than    3154 bp
  95% of sequence in fragments shorter than    4003 bp

# FauI.Tsp45I (CCCGC+GTSAC) double digest, 5461 thousand fragments (1439772+4021761 sites), 545 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     284 bp
  15% of sequence in fragments shorter than     372 bp
  20% of sequence in fragments shorter than     460 bp
  25% of sequence in fragments shorter than     549 bp
  30% of sequence in fragments shorter than     636 bp
  35% of sequence in fragments shorter than     727 bp
  40% of sequence in fragments shorter than     824 bp
  45% of sequence in fragments shorter than     925 bp
  50% of sequence in fragments shorter than    1034 bp
  55% of sequence in fragments shorter than    1153 bp
  60% of sequence in fragments shorter than    1285 bp
  65% of sequence in fragments shorter than    1432 bp
  70% of sequence in fragments shorter than    1605 bp
  75% of sequence in fragments shorter than    1806 bp
  80% of sequence in fragments shorter than    2052 bp
  85% of sequence in fragments shorter than    2365 bp
  90% of sequence in fragments shorter than    2833 bp
  95% of sequence in fragments shorter than    3711 bp

# BspMI.Tsp45I (ACCTGC+GTSAC) double digest, 5459 thousand fragments (1438129+4021761 sites), 545 bp average fragment length
   5% of sequence in fragments shorter than     189 bp
  10% of sequence in fragments shorter than     288 bp
  15% of sequence in fragments shorter than     369 bp
  20% of sequence in fragments shorter than     450 bp
  25% of sequence in fragments shorter than     532 bp
  30% of sequence in fragments shorter than     614 bp
  35% of sequence in fragments shorter than     695 bp
  40% of sequence in fragments shorter than     784 bp
  45% of sequence in fragments shorter than     874 bp
  50% of sequence in fragments shorter than     970 bp
  55% of sequence in fragments shorter than    1076 bp
  60% of sequence in fragments shorter than    1194 bp
  65% of sequence in fragments shorter than    1326 bp
  70% of sequence in fragments shorter than    1475 bp
  75% of sequence in fragments shorter than    1654 bp
  80% of sequence in fragments shorter than    1871 bp
  85% of sequence in fragments shorter than    2156 bp
  90% of sequence in fragments shorter than    2568 bp
  95% of sequence in fragments shorter than    3271 bp

# BstEII.CviQI (GGTNACC+GTAC) double digest, 5450 thousand fragments (335865+5115078 sites), 546 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     290 bp
  15% of sequence in fragments shorter than     379 bp
  20% of sequence in fragments shorter than     462 bp
  25% of sequence in fragments shorter than     540 bp
  30% of sequence in fragments shorter than     620 bp
  35% of sequence in fragments shorter than     698 bp
  40% of sequence in fragments shorter than     780 bp
  45% of sequence in fragments shorter than     867 bp
  50% of sequence in fragments shorter than     958 bp
  55% of sequence in fragments shorter than    1054 bp
  60% of sequence in fragments shorter than    1162 bp
  65% of sequence in fragments shorter than    1275 bp
  70% of sequence in fragments shorter than    1395 bp
  75% of sequence in fragments shorter than    1550 bp
  80% of sequence in fragments shorter than    1735 bp
  85% of sequence in fragments shorter than    1977 bp
  90% of sequence in fragments shorter than    2310 bp
  95% of sequence in fragments shorter than    2920 bp

# BbvI.PacI (GCAGC+TTAATTAA) double digest, 5449 thousand fragments (5290051+159455 sites), 546 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     294 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     499 bp
  25% of sequence in fragments shorter than     603 bp
  30% of sequence in fragments shorter than     707 bp
  35% of sequence in fragments shorter than     817 bp
  40% of sequence in fragments shorter than     925 bp
  45% of sequence in fragments shorter than    1043 bp
  50% of sequence in fragments shorter than    1175 bp
  55% of sequence in fragments shorter than    1313 bp
  60% of sequence in fragments shorter than    1466 bp
  65% of sequence in fragments shorter than    1627 bp
  70% of sequence in fragments shorter than    1817 bp
  75% of sequence in fragments shorter than    2045 bp
  80% of sequence in fragments shorter than    2308 bp
  85% of sequence in fragments shorter than    2666 bp
  90% of sequence in fragments shorter than    3148 bp
  95% of sequence in fragments shorter than    4015 bp

# BbvI.Hpy99I (GCAGC+CGWCG) double digest, 5447 thousand fragments (5290051+157917 sites), 547 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     293 bp
  15% of sequence in fragments shorter than     396 bp
  20% of sequence in fragments shorter than     503 bp
  25% of sequence in fragments shorter than     611 bp
  30% of sequence in fragments shorter than     719 bp
  35% of sequence in fragments shorter than     833 bp
  40% of sequence in fragments shorter than     947 bp
  45% of sequence in fragments shorter than    1072 bp
  50% of sequence in fragments shorter than    1208 bp
  55% of sequence in fragments shorter than    1353 bp
  60% of sequence in fragments shorter than    1514 bp
  65% of sequence in fragments shorter than    1683 bp
  70% of sequence in fragments shorter than    1887 bp
  75% of sequence in fragments shorter than    2111 bp
  80% of sequence in fragments shorter than    2406 bp
  85% of sequence in fragments shorter than    2778 bp
  90% of sequence in fragments shorter than    3296 bp
  95% of sequence in fragments shorter than    4212 bp

# ApaI.TfiI (GGGCCC+GAWTC) double digest, 5444 thousand fragments (460339+4984362 sites), 547 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     290 bp
  15% of sequence in fragments shorter than     356 bp
  20% of sequence in fragments shorter than     438 bp
  25% of sequence in fragments shorter than     519 bp
  30% of sequence in fragments shorter than     602 bp
  35% of sequence in fragments shorter than     683 bp
  40% of sequence in fragments shorter than     763 bp
  45% of sequence in fragments shorter than     846 bp
  50% of sequence in fragments shorter than     939 bp
  55% of sequence in fragments shorter than    1036 bp
  60% of sequence in fragments shorter than    1142 bp
  65% of sequence in fragments shorter than    1260 bp
  70% of sequence in fragments shorter than    1392 bp
  75% of sequence in fragments shorter than    1545 bp
  80% of sequence in fragments shorter than    1729 bp
  85% of sequence in fragments shorter than    1957 bp
  90% of sequence in fragments shorter than    2271 bp
  95% of sequence in fragments shorter than    2796 bp

# AciI.BscGI (CCGC+CCCGT) double digest, 5436 thousand fragments (4206130+1231070 sites), 548 bp average fragment length
   5% of sequence in fragments shorter than     166 bp
  10% of sequence in fragments shorter than     317 bp
  15% of sequence in fragments shorter than     441 bp
  20% of sequence in fragments shorter than     589 bp
  25% of sequence in fragments shorter than     740 bp
  30% of sequence in fragments shorter than     899 bp
  35% of sequence in fragments shorter than    1070 bp
  40% of sequence in fragments shorter than    1253 bp
  45% of sequence in fragments shorter than    1453 bp
  50% of sequence in fragments shorter than    1669 bp
  55% of sequence in fragments shorter than    1909 bp
  60% of sequence in fragments shorter than    2179 bp
  65% of sequence in fragments shorter than    2485 bp
  70% of sequence in fragments shorter than    2835 bp
  75% of sequence in fragments shorter than    3250 bp
  80% of sequence in fragments shorter than    3757 bp
  85% of sequence in fragments shorter than    4410 bp
  90% of sequence in fragments shorter than    5328 bp
  95% of sequence in fragments shorter than    6911 bp

# BsrBI.FokI (CCGCTC+GGATG) double digest, 5436 thousand fragments (235523+5201116 sites), 548 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     289 bp
  15% of sequence in fragments shorter than     378 bp
  20% of sequence in fragments shorter than     466 bp
  25% of sequence in fragments shorter than     553 bp
  30% of sequence in fragments shorter than     638 bp
  35% of sequence in fragments shorter than     727 bp
  40% of sequence in fragments shorter than     818 bp
  45% of sequence in fragments shorter than     915 bp
  50% of sequence in fragments shorter than    1012 bp
  55% of sequence in fragments shorter than    1117 bp
  60% of sequence in fragments shorter than    1232 bp
  65% of sequence in fragments shorter than    1363 bp
  70% of sequence in fragments shorter than    1507 bp
  75% of sequence in fragments shorter than    1682 bp
  80% of sequence in fragments shorter than    1889 bp
  85% of sequence in fragments shorter than    2161 bp
  90% of sequence in fragments shorter than    2545 bp
  95% of sequence in fragments shorter than    3232 bp

# HpaII.StyI (CCGG+CCWWGG) double digest, 5431 thousand fragments (2317722+3114107 sites), 548 bp average fragment length
   5% of sequence in fragments shorter than     168 bp
  10% of sequence in fragments shorter than     278 bp
  15% of sequence in fragments shorter than     382 bp
  20% of sequence in fragments shorter than     482 bp
  25% of sequence in fragments shorter than     579 bp
  30% of sequence in fragments shorter than     681 bp
  35% of sequence in fragments shorter than     778 bp
  40% of sequence in fragments shorter than     892 bp
  45% of sequence in fragments shorter than    1013 bp
  50% of sequence in fragments shorter than    1144 bp
  55% of sequence in fragments shorter than    1288 bp
  60% of sequence in fragments shorter than    1444 bp
  65% of sequence in fragments shorter than    1626 bp
  70% of sequence in fragments shorter than    1833 bp
  75% of sequence in fragments shorter than    2079 bp
  80% of sequence in fragments shorter than    2386 bp
  85% of sequence in fragments shorter than    2769 bp
  90% of sequence in fragments shorter than    3338 bp
  95% of sequence in fragments shorter than    4438 bp

# FokI.KasI (GGATG+GGCGCC) double digest, 5431 thousand fragments (5201116+230381 sites), 548 bp average fragment length
   5% of sequence in fragments shorter than     187 bp
  10% of sequence in fragments shorter than     289 bp
  15% of sequence in fragments shorter than     378 bp
  20% of sequence in fragments shorter than     466 bp
  25% of sequence in fragments shorter than     552 bp
  30% of sequence in fragments shorter than     638 bp
  35% of sequence in fragments shorter than     727 bp
  40% of sequence in fragments shorter than     819 bp
  45% of sequence in fragments shorter than     916 bp
  50% of sequence in fragments shorter than    1013 bp
  55% of sequence in fragments shorter than    1119 bp
  60% of sequence in fragments shorter than    1234 bp
  65% of sequence in fragments shorter than    1365 bp
  70% of sequence in fragments shorter than    1507 bp
  75% of sequence in fragments shorter than    1681 bp
  80% of sequence in fragments shorter than    1887 bp
  85% of sequence in fragments shorter than    2159 bp
  90% of sequence in fragments shorter than    2545 bp
  95% of sequence in fragments shorter than    3234 bp

# EcoRV.TfiI (GATATC+GAWTC) double digest, 5430 thousand fragments (446473+4984362 sites), 548 bp average fragment length
   5% of sequence in fragments shorter than     187 bp
  10% of sequence in fragments shorter than     290 bp
  15% of sequence in fragments shorter than     357 bp
  20% of sequence in fragments shorter than     440 bp
  25% of sequence in fragments shorter than     523 bp
  30% of sequence in fragments shorter than     606 bp
  35% of sequence in fragments shorter than     687 bp
  40% of sequence in fragments shorter than     770 bp
  45% of sequence in fragments shorter than     854 bp
  50% of sequence in fragments shorter than     947 bp
  55% of sequence in fragments shorter than    1046 bp
  60% of sequence in fragments shorter than    1156 bp
  65% of sequence in fragments shorter than    1276 bp
  70% of sequence in fragments shorter than    1412 bp
  75% of sequence in fragments shorter than    1569 bp
  80% of sequence in fragments shorter than    1758 bp
  85% of sequence in fragments shorter than    1991 bp
  90% of sequence in fragments shorter than    2320 bp
  95% of sequence in fragments shorter than    2871 bp

# FokI.SwaI (GGATG+ATTTAAAT) double digest, 5427 thousand fragments (5201116+226602 sites), 549 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     291 bp
  15% of sequence in fragments shorter than     378 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     549 bp
  30% of sequence in fragments shorter than     633 bp
  35% of sequence in fragments shorter than     719 bp
  40% of sequence in fragments shorter than     808 bp
  45% of sequence in fragments shorter than     901 bp
  50% of sequence in fragments shorter than     995 bp
  55% of sequence in fragments shorter than    1097 bp
  60% of sequence in fragments shorter than    1208 bp
  65% of sequence in fragments shorter than    1333 bp
  70% of sequence in fragments shorter than    1467 bp
  75% of sequence in fragments shorter than    1631 bp
  80% of sequence in fragments shorter than    1826 bp
  85% of sequence in fragments shorter than    2084 bp
  90% of sequence in fragments shorter than    2444 bp
  95% of sequence in fragments shorter than    3096 bp

# BbvI.SnaBI (GCAGC+TACGTA) double digest, 5421 thousand fragments (5290051+132299 sites), 549 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     295 bp
  15% of sequence in fragments shorter than     397 bp
  20% of sequence in fragments shorter than     501 bp
  25% of sequence in fragments shorter than     607 bp
  30% of sequence in fragments shorter than     713 bp
  35% of sequence in fragments shorter than     824 bp
  40% of sequence in fragments shorter than     933 bp
  45% of sequence in fragments shorter than    1054 bp
  50% of sequence in fragments shorter than    1187 bp
  55% of sequence in fragments shorter than    1327 bp
  60% of sequence in fragments shorter than    1482 bp
  65% of sequence in fragments shorter than    1646 bp
  70% of sequence in fragments shorter than    1842 bp
  75% of sequence in fragments shorter than    2067 bp
  80% of sequence in fragments shorter than    2343 bp
  85% of sequence in fragments shorter than    2706 bp
  90% of sequence in fragments shorter than    3199 bp
  95% of sequence in fragments shorter than    4078 bp

# HpaII.NspI (CCGG+RCATGY) double digest, 5421 thousand fragments (2317722+3104359 sites), 549 bp average fragment length
   5% of sequence in fragments shorter than     181 bp
  10% of sequence in fragments shorter than     290 bp
  15% of sequence in fragments shorter than     390 bp
  20% of sequence in fragments shorter than     482 bp
  25% of sequence in fragments shorter than     576 bp
  30% of sequence in fragments shorter than     670 bp
  35% of sequence in fragments shorter than     765 bp
  40% of sequence in fragments shorter than     865 bp
  45% of sequence in fragments shorter than     970 bp
  50% of sequence in fragments shorter than    1081 bp
  55% of sequence in fragments shorter than    1202 bp
  60% of sequence in fragments shorter than    1334 bp
  65% of sequence in fragments shorter than    1482 bp
  70% of sequence in fragments shorter than    1649 bp
  75% of sequence in fragments shorter than    1845 bp
  80% of sequence in fragments shorter than    2076 bp
  85% of sequence in fragments shorter than    2386 bp
  90% of sequence in fragments shorter than    2835 bp
  95% of sequence in fragments shorter than    3638 bp

# AflIII.MlyI (ACRYGT+GAGTC) double digest, 5416 thousand fragments (1485394+3931692 sites), 550 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     293 bp
  15% of sequence in fragments shorter than     374 bp
  20% of sequence in fragments shorter than     450 bp
  25% of sequence in fragments shorter than     528 bp
  30% of sequence in fragments shorter than     606 bp
  35% of sequence in fragments shorter than     685 bp
  40% of sequence in fragments shorter than     766 bp
  45% of sequence in fragments shorter than     852 bp
  50% of sequence in fragments shorter than     942 bp
  55% of sequence in fragments shorter than    1038 bp
  60% of sequence in fragments shorter than    1146 bp
  65% of sequence in fragments shorter than    1267 bp
  70% of sequence in fragments shorter than    1402 bp
  75% of sequence in fragments shorter than    1562 bp
  80% of sequence in fragments shorter than    1751 bp
  85% of sequence in fragments shorter than    2000 bp
  90% of sequence in fragments shorter than    2336 bp
  95% of sequence in fragments shorter than    2960 bp

# BbvI.NaeI (GCAGC+GCCGGC) double digest, 5415 thousand fragments (5290051+125692 sites), 550 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     295 bp
  15% of sequence in fragments shorter than     400 bp
  20% of sequence in fragments shorter than     508 bp
  25% of sequence in fragments shorter than     617 bp
  30% of sequence in fragments shorter than     726 bp
  35% of sequence in fragments shorter than     839 bp
  40% of sequence in fragments shorter than     955 bp
  45% of sequence in fragments shorter than    1081 bp
  50% of sequence in fragments shorter than    1218 bp
  55% of sequence in fragments shorter than    1365 bp
  60% of sequence in fragments shorter than    1528 bp
  65% of sequence in fragments shorter than    1698 bp
  70% of sequence in fragments shorter than    1903 bp
  75% of sequence in fragments shorter than    2130 bp
  80% of sequence in fragments shorter than    2426 bp
  85% of sequence in fragments shorter than    2801 bp
  90% of sequence in fragments shorter than    3325 bp
  95% of sequence in fragments shorter than    4251 bp

# CviQI.SexAI (GTAC+ACCWGGT) double digest, 5412 thousand fragments (5115078+297900 sites), 550 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     296 bp
  15% of sequence in fragments shorter than     384 bp
  20% of sequence in fragments shorter than     467 bp
  25% of sequence in fragments shorter than     545 bp
  30% of sequence in fragments shorter than     626 bp
  35% of sequence in fragments shorter than     705 bp
  40% of sequence in fragments shorter than     788 bp
  45% of sequence in fragments shorter than     876 bp
  50% of sequence in fragments shorter than     968 bp
  55% of sequence in fragments shorter than    1066 bp
  60% of sequence in fragments shorter than    1176 bp
  65% of sequence in fragments shorter than    1290 bp
  70% of sequence in fragments shorter than    1411 bp
  75% of sequence in fragments shorter than    1567 bp
  80% of sequence in fragments shorter than    1755 bp
  85% of sequence in fragments shorter than    2001 bp
  90% of sequence in fragments shorter than    2340 bp
  95% of sequence in fragments shorter than    2959 bp

# BbvI.XhoI (GCAGC+CTCGAG) double digest, 5410 thousand fragments (5290051+121308 sites), 550 bp average fragment length
   5% of sequence in fragments shorter than     182 bp
  10% of sequence in fragments shorter than     294 bp
  15% of sequence in fragments shorter than     397 bp
  20% of sequence in fragments shorter than     503 bp
  25% of sequence in fragments shorter than     609 bp
  30% of sequence in fragments shorter than     718 bp
  35% of sequence in fragments shorter than     832 bp
  40% of sequence in fragments shorter than     945 bp
  45% of sequence in fragments shorter than    1069 bp
  50% of sequence in fragments shorter than    1205 bp
  55% of sequence in fragments shorter than    1348 bp
  60% of sequence in fragments shorter than    1509 bp
  65% of sequence in fragments shorter than    1677 bp
  70% of sequence in fragments shorter than    1880 bp
  75% of sequence in fragments shorter than    2103 bp
  80% of sequence in fragments shorter than    2394 bp
  85% of sequence in fragments shorter than    2765 bp
  90% of sequence in fragments shorter than    3282 bp
  95% of sequence in fragments shorter than    4188 bp

# BscAI.HhaI (GCATC+GCGC) double digest, 5408 thousand fragments (3652926+1756502 sites), 550 bp average fragment length
   5% of sequence in fragments shorter than     184 bp
  10% of sequence in fragments shorter than     294 bp
  15% of sequence in fragments shorter than     388 bp
  20% of sequence in fragments shorter than     482 bp
  25% of sequence in fragments shorter than     573 bp
  30% of sequence in fragments shorter than     662 bp
  35% of sequence in fragments shorter than     748 bp
  40% of sequence in fragments shorter than     845 bp
  45% of sequence in fragments shorter than     946 bp
  50% of sequence in fragments shorter than    1053 bp
  55% of sequence in fragments shorter than    1171 bp
  60% of sequence in fragments shorter than    1298 bp
  65% of sequence in fragments shorter than    1435 bp
  70% of sequence in fragments shorter than    1594 bp
  75% of sequence in fragments shorter than    1773 bp
  80% of sequence in fragments shorter than    1993 bp
  85% of sequence in fragments shorter than    2275 bp
  90% of sequence in fragments shorter than    2666 bp
  95% of sequence in fragments shorter than    3333 bp

# CviQI.KpnI (GTAC+GGTACC) double digest, 5402 thousand fragments (5115078+287974 sites), 551 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     312 bp
  15% of sequence in fragments shorter than     406 bp
  20% of sequence in fragments shorter than     493 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     662 bp
  35% of sequence in fragments shorter than     746 bp
  40% of sequence in fragments shorter than     833 bp
  45% of sequence in fragments shorter than     926 bp
  50% of sequence in fragments shorter than    1025 bp
  55% of sequence in fragments shorter than    1129 bp
  60% of sequence in fragments shorter than    1240 bp
  65% of sequence in fragments shorter than    1359 bp
  70% of sequence in fragments shorter than    1497 bp
  75% of sequence in fragments shorter than    1663 bp
  80% of sequence in fragments shorter than    1869 bp
  85% of sequence in fragments shorter than    2128 bp
  90% of sequence in fragments shorter than    2499 bp
  95% of sequence in fragments shorter than    3169 bp

# CviQI.NheI (GTAC+GCTAGC) double digest, 5401 thousand fragments (5115078+287384 sites), 551 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     293 bp
  15% of sequence in fragments shorter than     381 bp
  20% of sequence in fragments shorter than     464 bp
  25% of sequence in fragments shorter than     542 bp
  30% of sequence in fragments shorter than     623 bp
  35% of sequence in fragments shorter than     706 bp
  40% of sequence in fragments shorter than     790 bp
  45% of sequence in fragments shorter than     879 bp
  50% of sequence in fragments shorter than     971 bp
  55% of sequence in fragments shorter than    1071 bp
  60% of sequence in fragments shorter than    1180 bp
  65% of sequence in fragments shorter than    1286 bp
  70% of sequence in fragments shorter than    1418 bp
  75% of sequence in fragments shorter than    1576 bp
  80% of sequence in fragments shorter than    1769 bp
  85% of sequence in fragments shorter than    2020 bp
  90% of sequence in fragments shorter than    2370 bp
  95% of sequence in fragments shorter than    3009 bp

# SpeI.TfiI (ACTAGT+GAWTC) double digest, 5384 thousand fragments (400286+4984362 sites), 553 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     292 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     444 bp
  25% of sequence in fragments shorter than     527 bp
  30% of sequence in fragments shorter than     611 bp
  35% of sequence in fragments shorter than     691 bp
  40% of sequence in fragments shorter than     775 bp
  45% of sequence in fragments shorter than     860 bp
  50% of sequence in fragments shorter than     954 bp
  55% of sequence in fragments shorter than    1054 bp
  60% of sequence in fragments shorter than    1164 bp
  65% of sequence in fragments shorter than    1285 bp
  70% of sequence in fragments shorter than    1422 bp
  75% of sequence in fragments shorter than    1581 bp
  80% of sequence in fragments shorter than    1772 bp
  85% of sequence in fragments shorter than    2007 bp
  90% of sequence in fragments shorter than    2339 bp
  95% of sequence in fragments shorter than    2895 bp

# BbvI.ClaI (GCAGC+ATCGAT) double digest, 5376 thousand fragments (5290051+86477 sites), 554 bp average fragment length
   5% of sequence in fragments shorter than     184 bp
  10% of sequence in fragments shorter than     297 bp
  15% of sequence in fragments shorter than     400 bp
  20% of sequence in fragments shorter than     507 bp
  25% of sequence in fragments shorter than     614 bp
  30% of sequence in fragments shorter than     721 bp
  35% of sequence in fragments shorter than     833 bp
  40% of sequence in fragments shorter than     946 bp
  45% of sequence in fragments shorter than    1069 bp
  50% of sequence in fragments shorter than    1205 bp
  55% of sequence in fragments shorter than    1347 bp
  60% of sequence in fragments shorter than    1506 bp
  65% of sequence in fragments shorter than    1671 bp
  70% of sequence in fragments shorter than    1871 bp
  75% of sequence in fragments shorter than    2092 bp
  80% of sequence in fragments shorter than    2381 bp
  85% of sequence in fragments shorter than    2751 bp
  90% of sequence in fragments shorter than    3257 bp
  95% of sequence in fragments shorter than    4147 bp

# AclI.FokI (AACGTT+GGATG) double digest, 5373 thousand fragments (172706+5201116 sites), 554 bp average fragment length
   5% of sequence in fragments shorter than     192 bp
  10% of sequence in fragments shorter than     294 bp
  15% of sequence in fragments shorter than     383 bp
  20% of sequence in fragments shorter than     470 bp
  25% of sequence in fragments shorter than     555 bp
  30% of sequence in fragments shorter than     640 bp
  35% of sequence in fragments shorter than     728 bp
  40% of sequence in fragments shorter than     819 bp
  45% of sequence in fragments shorter than     914 bp
  50% of sequence in fragments shorter than    1013 bp
  55% of sequence in fragments shorter than    1115 bp
  60% of sequence in fragments shorter than    1228 bp
  65% of sequence in fragments shorter than    1356 bp
  70% of sequence in fragments shorter than    1495 bp
  75% of sequence in fragments shorter than    1664 bp
  80% of sequence in fragments shorter than    1866 bp
  85% of sequence in fragments shorter than    2127 bp
  90% of sequence in fragments shorter than    2497 bp
  95% of sequence in fragments shorter than    3139 bp

# HpaI.TfiI (GTTAAC+GAWTC) double digest, 5373 thousand fragments (389418+4984362 sites), 554 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     293 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     442 bp
  25% of sequence in fragments shorter than     525 bp
  30% of sequence in fragments shorter than     610 bp
  35% of sequence in fragments shorter than     690 bp
  40% of sequence in fragments shorter than     773 bp
  45% of sequence in fragments shorter than     858 bp
  50% of sequence in fragments shorter than     952 bp
  55% of sequence in fragments shorter than    1051 bp
  60% of sequence in fragments shorter than    1160 bp
  65% of sequence in fragments shorter than    1281 bp
  70% of sequence in fragments shorter than    1417 bp
  75% of sequence in fragments shorter than    1575 bp
  80% of sequence in fragments shorter than    1764 bp
  85% of sequence in fragments shorter than    2000 bp
  90% of sequence in fragments shorter than    2330 bp
  95% of sequence in fragments shorter than    2885 bp

# FauI.MlyI (CCCGC+GAGTC) double digest, 5370 thousand fragments (1439772+3931692 sites), 554 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     282 bp
  15% of sequence in fragments shorter than     377 bp
  20% of sequence in fragments shorter than     471 bp
  25% of sequence in fragments shorter than     564 bp
  30% of sequence in fragments shorter than     659 bp
  35% of sequence in fragments shorter than     755 bp
  40% of sequence in fragments shorter than     858 bp
  45% of sequence in fragments shorter than     963 bp
  50% of sequence in fragments shorter than    1078 bp
  55% of sequence in fragments shorter than    1203 bp
  60% of sequence in fragments shorter than    1343 bp
  65% of sequence in fragments shorter than    1496 bp
  70% of sequence in fragments shorter than    1673 bp
  75% of sequence in fragments shorter than    1880 bp
  80% of sequence in fragments shorter than    2133 bp
  85% of sequence in fragments shorter than    2473 bp
  90% of sequence in fragments shorter than    2956 bp
  95% of sequence in fragments shorter than    3854 bp

# BspMI.MlyI (ACCTGC+GAGTC) double digest, 5369 thousand fragments (1438129+3931692 sites), 555 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     288 bp
  15% of sequence in fragments shorter than     366 bp
  20% of sequence in fragments shorter than     450 bp
  25% of sequence in fragments shorter than     531 bp
  30% of sequence in fragments shorter than     614 bp
  35% of sequence in fragments shorter than     695 bp
  40% of sequence in fragments shorter than     782 bp
  45% of sequence in fragments shorter than     872 bp
  50% of sequence in fragments shorter than     972 bp
  55% of sequence in fragments shorter than    1080 bp
  60% of sequence in fragments shorter than    1200 bp
  65% of sequence in fragments shorter than    1327 bp
  70% of sequence in fragments shorter than    1471 bp
  75% of sequence in fragments shorter than    1648 bp
  80% of sequence in fragments shorter than    1865 bp
  85% of sequence in fragments shorter than    2142 bp
  90% of sequence in fragments shorter than    2546 bp
  95% of sequence in fragments shorter than    3202 bp

# SapI.TfiI (GCTCTTC+GAWTC) double digest, 5361 thousand fragments (377161+4984362 sites), 555 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     295 bp
  15% of sequence in fragments shorter than     362 bp
  20% of sequence in fragments shorter than     447 bp
  25% of sequence in fragments shorter than     529 bp
  30% of sequence in fragments shorter than     614 bp
  35% of sequence in fragments shorter than     693 bp
  40% of sequence in fragments shorter than     776 bp
  45% of sequence in fragments shorter than     863 bp
  50% of sequence in fragments shorter than     958 bp
  55% of sequence in fragments shorter than    1058 bp
  60% of sequence in fragments shorter than    1167 bp
  65% of sequence in fragments shorter than    1288 bp
  70% of sequence in fragments shorter than    1424 bp
  75% of sequence in fragments shorter than    1582 bp
  80% of sequence in fragments shorter than    1771 bp
  85% of sequence in fragments shorter than    2005 bp
  90% of sequence in fragments shorter than    2331 bp
  95% of sequence in fragments shorter than    2878 bp

# SmaI.TfiI (CCCGGG+GAWTC) double digest, 5360 thousand fragments (376940+4984362 sites), 555 bp average fragment length
   5% of sequence in fragments shorter than     194 bp
  10% of sequence in fragments shorter than     298 bp
  15% of sequence in fragments shorter than     364 bp
  20% of sequence in fragments shorter than     449 bp
  25% of sequence in fragments shorter than     531 bp
  30% of sequence in fragments shorter than     615 bp
  35% of sequence in fragments shorter than     694 bp
  40% of sequence in fragments shorter than     779 bp
  45% of sequence in fragments shorter than     864 bp
  50% of sequence in fragments shorter than     958 bp
  55% of sequence in fragments shorter than    1057 bp
  60% of sequence in fragments shorter than    1165 bp
  65% of sequence in fragments shorter than    1285 bp
  70% of sequence in fragments shorter than    1420 bp
  75% of sequence in fragments shorter than    1576 bp
  80% of sequence in fragments shorter than    1763 bp
  85% of sequence in fragments shorter than    1993 bp
  90% of sequence in fragments shorter than    2313 bp
  95% of sequence in fragments shorter than    2847 bp

# AatII.BbvI (GACGTC+GCAGC) double digest, 5360 thousand fragments (71089+5290051 sites), 555 bp average fragment length
   5% of sequence in fragments shorter than     184 bp
  10% of sequence in fragments shorter than     297 bp
  15% of sequence in fragments shorter than     402 bp
  20% of sequence in fragments shorter than     509 bp
  25% of sequence in fragments shorter than     617 bp
  30% of sequence in fragments shorter than     726 bp
  35% of sequence in fragments shorter than     838 bp
  40% of sequence in fragments shorter than     954 bp
  45% of sequence in fragments shorter than    1079 bp
  50% of sequence in fragments shorter than    1215 bp
  55% of sequence in fragments shorter than    1361 bp
  60% of sequence in fragments shorter than    1523 bp
  65% of sequence in fragments shorter than    1693 bp
  70% of sequence in fragments shorter than    1896 bp
  75% of sequence in fragments shorter than    2121 bp
  80% of sequence in fragments shorter than    2415 bp
  85% of sequence in fragments shorter than    2787 bp
  90% of sequence in fragments shorter than    3308 bp
  95% of sequence in fragments shorter than    4224 bp

# FokI.PacI (GGATG+TTAATTAA) double digest, 5360 thousand fragments (5201116+159455 sites), 555 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     296 bp
  15% of sequence in fragments shorter than     384 bp
  20% of sequence in fragments shorter than     472 bp
  25% of sequence in fragments shorter than     558 bp
  30% of sequence in fragments shorter than     643 bp
  35% of sequence in fragments shorter than     731 bp
  40% of sequence in fragments shorter than     822 bp
  45% of sequence in fragments shorter than     917 bp
  50% of sequence in fragments shorter than    1013 bp
  55% of sequence in fragments shorter than    1117 bp
  60% of sequence in fragments shorter than    1230 bp
  65% of sequence in fragments shorter than    1359 bp
  70% of sequence in fragments shorter than    1498 bp
  75% of sequence in fragments shorter than    1668 bp
  80% of sequence in fragments shorter than    1869 bp
  85% of sequence in fragments shorter than    2132 bp
  90% of sequence in fragments shorter than    2507 bp
  95% of sequence in fragments shorter than    3173 bp

# BfiI.BscAI (ACTGGG+GCATC) double digest, 5359 thousand fragments (1706594+3652926 sites), 556 bp average fragment length
   5% of sequence in fragments shorter than     187 bp
  10% of sequence in fragments shorter than     289 bp
  15% of sequence in fragments shorter than     378 bp
  20% of sequence in fragments shorter than     465 bp
  25% of sequence in fragments shorter than     550 bp
  30% of sequence in fragments shorter than     633 bp
  35% of sequence in fragments shorter than     717 bp
  40% of sequence in fragments shorter than     801 bp
  45% of sequence in fragments shorter than     892 bp
  50% of sequence in fragments shorter than     987 bp
  55% of sequence in fragments shorter than    1094 bp
  60% of sequence in fragments shorter than    1210 bp
  65% of sequence in fragments shorter than    1340 bp
  70% of sequence in fragments shorter than    1483 bp
  75% of sequence in fragments shorter than    1655 bp
  80% of sequence in fragments shorter than    1864 bp
  85% of sequence in fragments shorter than    2129 bp
  90% of sequence in fragments shorter than    2509 bp
  95% of sequence in fragments shorter than    3189 bp

# FokI.Hpy99I (GGATG+CGWCG) double digest, 5358 thousand fragments (5201116+157917 sites), 556 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     294 bp
  15% of sequence in fragments shorter than     383 bp
  20% of sequence in fragments shorter than     473 bp
  25% of sequence in fragments shorter than     560 bp
  30% of sequence in fragments shorter than     647 bp
  35% of sequence in fragments shorter than     736 bp
  40% of sequence in fragments shorter than     829 bp
  45% of sequence in fragments shorter than     926 bp
  50% of sequence in fragments shorter than    1026 bp
  55% of sequence in fragments shorter than    1132 bp
  60% of sequence in fragments shorter than    1248 bp
  65% of sequence in fragments shorter than    1380 bp
  70% of sequence in fragments shorter than    1525 bp
  75% of sequence in fragments shorter than    1700 bp
  80% of sequence in fragments shorter than    1909 bp
  85% of sequence in fragments shorter than    2184 bp
  90% of sequence in fragments shorter than    2573 bp
  95% of sequence in fragments shorter than    3270 bp

# BbvI.SacII (GCAGC+CCGCGG) double digest, 5356 thousand fragments (5290051+66901 sites), 556 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     298 bp
  15% of sequence in fragments shorter than     404 bp
  20% of sequence in fragments shorter than     513 bp
  25% of sequence in fragments shorter than     623 bp
  30% of sequence in fragments shorter than     733 bp
  35% of sequence in fragments shorter than     847 bp
  40% of sequence in fragments shorter than     964 bp
  45% of sequence in fragments shorter than    1090 bp
  50% of sequence in fragments shorter than    1227 bp
  55% of sequence in fragments shorter than    1376 bp
  60% of sequence in fragments shorter than    1540 bp
  65% of sequence in fragments shorter than    1711 bp
  70% of sequence in fragments shorter than    1917 bp
  75% of sequence in fragments shorter than    2146 bp
  80% of sequence in fragments shorter than    2444 bp
  85% of sequence in fragments shorter than    2822 bp
  90% of sequence in fragments shorter than    3349 bp
  95% of sequence in fragments shorter than    4278 bp

# BsrBI.CviQI (CCGCTC+GTAC) double digest, 5350 thousand fragments (235523+5115078 sites), 556 bp average fragment length
   5% of sequence in fragments shorter than     195 bp
  10% of sequence in fragments shorter than     299 bp
  15% of sequence in fragments shorter than     388 bp
  20% of sequence in fragments shorter than     471 bp
  25% of sequence in fragments shorter than     550 bp
  30% of sequence in fragments shorter than     631 bp
  35% of sequence in fragments shorter than     711 bp
  40% of sequence in fragments shorter than     794 bp
  45% of sequence in fragments shorter than     882 bp
  50% of sequence in fragments shorter than     975 bp
  55% of sequence in fragments shorter than    1072 bp
  60% of sequence in fragments shorter than    1183 bp
  65% of sequence in fragments shorter than    1296 bp
  70% of sequence in fragments shorter than    1417 bp
  75% of sequence in fragments shorter than    1573 bp
  80% of sequence in fragments shorter than    1761 bp
  85% of sequence in fragments shorter than    2005 bp
  90% of sequence in fragments shorter than    2340 bp
  95% of sequence in fragments shorter than    2940 bp

# BamHI.TfiI (GGATCC+GAWTC) double digest, 5349 thousand fragments (365999+4984362 sites), 557 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     296 bp
  15% of sequence in fragments shorter than     360 bp
  20% of sequence in fragments shorter than     447 bp
  25% of sequence in fragments shorter than     530 bp
  30% of sequence in fragments shorter than     615 bp
  35% of sequence in fragments shorter than     694 bp
  40% of sequence in fragments shorter than     780 bp
  45% of sequence in fragments shorter than     866 bp
  50% of sequence in fragments shorter than     961 bp
  55% of sequence in fragments shorter than    1060 bp
  60% of sequence in fragments shorter than    1169 bp
  65% of sequence in fragments shorter than    1289 bp
  70% of sequence in fragments shorter than    1425 bp
  75% of sequence in fragments shorter than    1582 bp
  80% of sequence in fragments shorter than    1770 bp
  85% of sequence in fragments shorter than    2002 bp
  90% of sequence in fragments shorter than    2327 bp
  95% of sequence in fragments shorter than    2869 bp

# BbvI.PspXI (GCAGC+VCTCGAGB) double digest, 5348 thousand fragments (5290051+59193 sites), 557 bp average fragment length
   5% of sequence in fragments shorter than     184 bp
  10% of sequence in fragments shorter than     298 bp
  15% of sequence in fragments shorter than     403 bp
  20% of sequence in fragments shorter than     511 bp
  25% of sequence in fragments shorter than     619 bp
  30% of sequence in fragments shorter than     729 bp
  35% of sequence in fragments shorter than     842 bp
  40% of sequence in fragments shorter than     957 bp
  45% of sequence in fragments shorter than    1083 bp
  50% of sequence in fragments shorter than    1219 bp
  55% of sequence in fragments shorter than    1366 bp
  60% of sequence in fragments shorter than    1529 bp
  65% of sequence in fragments shorter than    1699 bp
  70% of sequence in fragments shorter than    1904 bp
  75% of sequence in fragments shorter than    2131 bp
  80% of sequence in fragments shorter than    2426 bp
  85% of sequence in fragments shorter than    2801 bp
  90% of sequence in fragments shorter than    3324 bp
  95% of sequence in fragments shorter than    4246 bp

# CviQI.KasI (GTAC+GGCGCC) double digest, 5344 thousand fragments (5115078+230381 sites), 557 bp average fragment length
   5% of sequence in fragments shorter than     196 bp
  10% of sequence in fragments shorter than     298 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     468 bp
  25% of sequence in fragments shorter than     546 bp
  30% of sequence in fragments shorter than     627 bp
  35% of sequence in fragments shorter than     706 bp
  40% of sequence in fragments shorter than     790 bp
  45% of sequence in fragments shorter than     878 bp
  50% of sequence in fragments shorter than     969 bp
  55% of sequence in fragments shorter than    1066 bp
  60% of sequence in fragments shorter than    1176 bp
  65% of sequence in fragments shorter than    1289 bp
  70% of sequence in fragments shorter than    1409 bp
  75% of sequence in fragments shorter than    1565 bp
  80% of sequence in fragments shorter than    1752 bp
  85% of sequence in fragments shorter than    1995 bp
  90% of sequence in fragments shorter than    2330 bp
  95% of sequence in fragments shorter than    2937 bp

# AgeI.BbvI (ACCGGT+GCAGC) double digest, 5342 thousand fragments (53386+5290051 sites), 557 bp average fragment length
   5% of sequence in fragments shorter than     185 bp
  10% of sequence in fragments shorter than     298 bp
  15% of sequence in fragments shorter than     403 bp
  20% of sequence in fragments shorter than     511 bp
  25% of sequence in fragments shorter than     619 bp
  30% of sequence in fragments shorter than     728 bp
  35% of sequence in fragments shorter than     841 bp
  40% of sequence in fragments shorter than     957 bp
  45% of sequence in fragments shorter than    1082 bp
  50% of sequence in fragments shorter than    1219 bp
  55% of sequence in fragments shorter than    1365 bp
  60% of sequence in fragments shorter than    1527 bp
  65% of sequence in fragments shorter than    1697 bp
  70% of sequence in fragments shorter than    1897 bp
  75% of sequence in fragments shorter than    2127 bp
  80% of sequence in fragments shorter than    2422 bp
  85% of sequence in fragments shorter than    2797 bp
  90% of sequence in fragments shorter than    3320 bp
  95% of sequence in fragments shorter than    4241 bp

# PstI.Tsp45I (CTGCAG+GTSAC) double digest, 5342 thousand fragments (1321469+4021761 sites), 557 bp average fragment length
   5% of sequence in fragments shorter than     192 bp
  10% of sequence in fragments shorter than     293 bp
  15% of sequence in fragments shorter than     376 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     544 bp
  30% of sequence in fragments shorter than     626 bp
  35% of sequence in fragments shorter than     709 bp
  40% of sequence in fragments shorter than     801 bp
  45% of sequence in fragments shorter than     895 bp
  50% of sequence in fragments shorter than     996 bp
  55% of sequence in fragments shorter than    1107 bp
  60% of sequence in fragments shorter than    1231 bp
  65% of sequence in fragments shorter than    1370 bp
  70% of sequence in fragments shorter than    1533 bp
  75% of sequence in fragments shorter than    1723 bp
  80% of sequence in fragments shorter than    1959 bp
  85% of sequence in fragments shorter than    2272 bp
  90% of sequence in fragments shorter than    2734 bp
  95% of sequence in fragments shorter than    3625 bp

# CviQI.SwaI (GTAC+ATTTAAAT) double digest, 5341 thousand fragments (5115078+226602 sites), 557 bp average fragment length
   5% of sequence in fragments shorter than     194 bp
  10% of sequence in fragments shorter than     298 bp
  15% of sequence in fragments shorter than     388 bp
  20% of sequence in fragments shorter than     471 bp
  25% of sequence in fragments shorter than     550 bp
  30% of sequence in fragments shorter than     633 bp
  35% of sequence in fragments shorter than     713 bp
  40% of sequence in fragments shorter than     797 bp
  45% of sequence in fragments shorter than     887 bp
  50% of sequence in fragments shorter than     981 bp
  55% of sequence in fragments shorter than    1081 bp
  60% of sequence in fragments shorter than    1194 bp
  65% of sequence in fragments shorter than    1311 bp
  70% of sequence in fragments shorter than    1436 bp
  75% of sequence in fragments shorter than    1597 bp
  80% of sequence in fragments shorter than    1794 bp
  85% of sequence in fragments shorter than    2051 bp
  90% of sequence in fragments shorter than    2409 bp
  95% of sequence in fragments shorter than    3067 bp

# FokI.SnaBI (GGATG+TACGTA) double digest, 5332 thousand fragments (5201116+132299 sites), 558 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     296 bp
  15% of sequence in fragments shorter than     385 bp
  20% of sequence in fragments shorter than     474 bp
  25% of sequence in fragments shorter than     561 bp
  30% of sequence in fragments shorter than     648 bp
  35% of sequence in fragments shorter than     737 bp
  40% of sequence in fragments shorter than     828 bp
  45% of sequence in fragments shorter than     925 bp
  50% of sequence in fragments shorter than    1023 bp
  55% of sequence in fragments shorter than    1128 bp
  60% of sequence in fragments shorter than    1242 bp
  65% of sequence in fragments shorter than    1369 bp
  70% of sequence in fragments shorter than    1512 bp
  75% of sequence in fragments shorter than    1684 bp
  80% of sequence in fragments shorter than    1888 bp
  85% of sequence in fragments shorter than    2156 bp
  90% of sequence in fragments shorter than    2536 bp
  95% of sequence in fragments shorter than    3214 bp

# BbvI.PmeI (GCAGC+GTTTAAAC) double digest, 5331 thousand fragments (5290051+42094 sites), 558 bp average fragment length
   5% of sequence in fragments shorter than     185 bp
  10% of sequence in fragments shorter than     299 bp
  15% of sequence in fragments shorter than     404 bp
  20% of sequence in fragments shorter than     511 bp
  25% of sequence in fragments shorter than     619 bp
  30% of sequence in fragments shorter than     728 bp
  35% of sequence in fragments shorter than     841 bp
  40% of sequence in fragments shorter than     956 bp
  45% of sequence in fragments shorter than    1081 bp
  50% of sequence in fragments shorter than    1216 bp
  55% of sequence in fragments shorter than    1362 bp
  60% of sequence in fragments shorter than    1523 bp
  65% of sequence in fragments shorter than    1692 bp
  70% of sequence in fragments shorter than    1895 bp
  75% of sequence in fragments shorter than    2118 bp
  80% of sequence in fragments shorter than    2411 bp
  85% of sequence in fragments shorter than    2782 bp
  90% of sequence in fragments shorter than    3299 bp
  95% of sequence in fragments shorter than    4210 bp

# FokI.NaeI (GGATG+GCCGGC) double digest, 5326 thousand fragments (5201116+125692 sites), 559 bp average fragment length
   5% of sequence in fragments shorter than     192 bp
  10% of sequence in fragments shorter than     296 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     475 bp
  25% of sequence in fragments shorter than     563 bp
  30% of sequence in fragments shorter than     651 bp
  35% of sequence in fragments shorter than     741 bp
  40% of sequence in fragments shorter than     834 bp
  45% of sequence in fragments shorter than     929 bp
  50% of sequence in fragments shorter than    1032 bp
  55% of sequence in fragments shorter than    1140 bp
  60% of sequence in fragments shorter than    1257 bp
  65% of sequence in fragments shorter than    1385 bp
  70% of sequence in fragments shorter than    1534 bp
  75% of sequence in fragments shorter than    1711 bp
  80% of sequence in fragments shorter than    1921 bp
  85% of sequence in fragments shorter than    2199 bp
  90% of sequence in fragments shorter than    2590 bp
  95% of sequence in fragments shorter than    3295 bp

# FokI.XhoI (GGATG+CTCGAG) double digest, 5321 thousand fragments (5201116+121308 sites), 559 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     297 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     475 bp
  25% of sequence in fragments shorter than     562 bp
  30% of sequence in fragments shorter than     647 bp
  35% of sequence in fragments shorter than     736 bp
  40% of sequence in fragments shorter than     829 bp
  45% of sequence in fragments shorter than     926 bp
  50% of sequence in fragments shorter than    1025 bp
  55% of sequence in fragments shorter than    1132 bp
  60% of sequence in fragments shorter than    1249 bp
  65% of sequence in fragments shorter than    1381 bp
  70% of sequence in fragments shorter than    1525 bp
  75% of sequence in fragments shorter than    1701 bp
  80% of sequence in fragments shorter than    1908 bp
  85% of sequence in fragments shorter than    2181 bp
  90% of sequence in fragments shorter than    2568 bp
  95% of sequence in fragments shorter than    3264 bp

# BbvI.SalI (GCAGC+GTCGAC) double digest, 5320 thousand fragments (5290051+31396 sites), 560 bp average fragment length
   5% of sequence in fragments shorter than     185 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     513 bp
  25% of sequence in fragments shorter than     622 bp
  30% of sequence in fragments shorter than     732 bp
  35% of sequence in fragments shorter than     845 bp
  40% of sequence in fragments shorter than     961 bp
  45% of sequence in fragments shorter than    1087 bp
  50% of sequence in fragments shorter than    1224 bp
  55% of sequence in fragments shorter than    1371 bp
  60% of sequence in fragments shorter than    1534 bp
  65% of sequence in fragments shorter than    1704 bp
  70% of sequence in fragments shorter than    1909 bp
  75% of sequence in fragments shorter than    2136 bp
  80% of sequence in fragments shorter than    2432 bp
  85% of sequence in fragments shorter than    2808 bp
  90% of sequence in fragments shorter than    3332 bp
  95% of sequence in fragments shorter than    4256 bp

# BstEII.TfiI (GGTNACC+GAWTC) double digest, 5319 thousand fragments (335865+4984362 sites), 560 bp average fragment length
   5% of sequence in fragments shorter than     192 bp
  10% of sequence in fragments shorter than     297 bp
  15% of sequence in fragments shorter than     365 bp
  20% of sequence in fragments shorter than     450 bp
  25% of sequence in fragments shorter than     533 bp
  30% of sequence in fragments shorter than     618 bp
  35% of sequence in fragments shorter than     697 bp
  40% of sequence in fragments shorter than     780 bp
  45% of sequence in fragments shorter than     868 bp
  50% of sequence in fragments shorter than     964 bp
  55% of sequence in fragments shorter than    1063 bp
  60% of sequence in fragments shorter than    1173 bp
  65% of sequence in fragments shorter than    1294 bp
  70% of sequence in fragments shorter than    1431 bp
  75% of sequence in fragments shorter than    1590 bp
  80% of sequence in fragments shorter than    1779 bp
  85% of sequence in fragments shorter than    2012 bp
  90% of sequence in fragments shorter than    2337 bp
  95% of sequence in fragments shorter than    2879 bp

# BbvI.MluI (GCAGC+ACGCGT) double digest, 5311 thousand fragments (5290051+21415 sites), 561 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     406 bp
  20% of sequence in fragments shorter than     514 bp
  25% of sequence in fragments shorter than     624 bp
  30% of sequence in fragments shorter than     734 bp
  35% of sequence in fragments shorter than     847 bp
  40% of sequence in fragments shorter than     963 bp
  45% of sequence in fragments shorter than    1090 bp
  50% of sequence in fragments shorter than    1226 bp
  55% of sequence in fragments shorter than    1375 bp
  60% of sequence in fragments shorter than    1539 bp
  65% of sequence in fragments shorter than    1709 bp
  70% of sequence in fragments shorter than    1915 bp
  75% of sequence in fragments shorter than    2143 bp
  80% of sequence in fragments shorter than    2441 bp
  85% of sequence in fragments shorter than    2818 bp
  90% of sequence in fragments shorter than    3345 bp
  95% of sequence in fragments shorter than    4272 bp

# AccI.AciI (GTMKAC+CCGC) double digest, 5306 thousand fragments (1101063+4206130 sites), 561 bp average fragment length
   5% of sequence in fragments shorter than     181 bp
  10% of sequence in fragments shorter than     326 bp
  15% of sequence in fragments shorter than     454 bp
  20% of sequence in fragments shorter than     578 bp
  25% of sequence in fragments shorter than     704 bp
  30% of sequence in fragments shorter than     832 bp
  35% of sequence in fragments shorter than     958 bp
  40% of sequence in fragments shorter than    1090 bp
  45% of sequence in fragments shorter than    1234 bp
  50% of sequence in fragments shorter than    1383 bp
  55% of sequence in fragments shorter than    1545 bp
  60% of sequence in fragments shorter than    1728 bp
  65% of sequence in fragments shorter than    1933 bp
  70% of sequence in fragments shorter than    2167 bp
  75% of sequence in fragments shorter than    2441 bp
  80% of sequence in fragments shorter than    2770 bp
  85% of sequence in fragments shorter than    3192 bp
  90% of sequence in fragments shorter than    3788 bp
  95% of sequence in fragments shorter than    4800 bp

# BbvI.SgrAI (GCAGC+CRCCGGYG) double digest, 5304 thousand fragments (5290051+15270 sites), 561 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     301 bp
  15% of sequence in fragments shorter than     407 bp
  20% of sequence in fragments shorter than     516 bp
  25% of sequence in fragments shorter than     625 bp
  30% of sequence in fragments shorter than     736 bp
  35% of sequence in fragments shorter than     849 bp
  40% of sequence in fragments shorter than     966 bp
  45% of sequence in fragments shorter than    1092 bp
  50% of sequence in fragments shorter than    1229 bp
  55% of sequence in fragments shorter than    1378 bp
  60% of sequence in fragments shorter than    1542 bp
  65% of sequence in fragments shorter than    1714 bp
  70% of sequence in fragments shorter than    1919 bp
  75% of sequence in fragments shorter than    2149 bp
  80% of sequence in fragments shorter than    2447 bp
  85% of sequence in fragments shorter than    2824 bp
  90% of sequence in fragments shorter than    3352 bp
  95% of sequence in fragments shorter than    4283 bp

# BbvI.NruI (GCAGC+TCGCGA) double digest, 5304 thousand fragments (5290051+14872 sites), 561 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     301 bp
  15% of sequence in fragments shorter than     407 bp
  20% of sequence in fragments shorter than     515 bp
  25% of sequence in fragments shorter than     624 bp
  30% of sequence in fragments shorter than     734 bp
  35% of sequence in fragments shorter than     848 bp
  40% of sequence in fragments shorter than     965 bp
  45% of sequence in fragments shorter than    1091 bp
  50% of sequence in fragments shorter than    1227 bp
  55% of sequence in fragments shorter than    1376 bp
  60% of sequence in fragments shorter than    1540 bp
  65% of sequence in fragments shorter than    1711 bp
  70% of sequence in fragments shorter than    1916 bp
  75% of sequence in fragments shorter than    2145 bp
  80% of sequence in fragments shorter than    2442 bp
  85% of sequence in fragments shorter than    2820 bp
  90% of sequence in fragments shorter than    3346 bp
  95% of sequence in fragments shorter than    4276 bp

# BbvI.PvuI (GCAGC+CGATCG) double digest, 5303 thousand fragments (5290051+14186 sites), 561 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     407 bp
  20% of sequence in fragments shorter than     515 bp
  25% of sequence in fragments shorter than     625 bp
  30% of sequence in fragments shorter than     735 bp
  35% of sequence in fragments shorter than     849 bp
  40% of sequence in fragments shorter than     965 bp
  45% of sequence in fragments shorter than    1091 bp
  50% of sequence in fragments shorter than    1228 bp
  55% of sequence in fragments shorter than    1377 bp
  60% of sequence in fragments shorter than    1541 bp
  65% of sequence in fragments shorter than    1712 bp
  70% of sequence in fragments shorter than    1917 bp
  75% of sequence in fragments shorter than    2147 bp
  80% of sequence in fragments shorter than    2444 bp
  85% of sequence in fragments shorter than    2822 bp
  90% of sequence in fragments shorter than    3348 bp
  95% of sequence in fragments shorter than    4275 bp

# BbvI.FseI (GCAGC+GGCCGGCC) double digest, 5303 thousand fragments (5290051+13662 sites), 561 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     301 bp
  15% of sequence in fragments shorter than     407 bp
  20% of sequence in fragments shorter than     516 bp
  25% of sequence in fragments shorter than     625 bp
  30% of sequence in fragments shorter than     736 bp
  35% of sequence in fragments shorter than     849 bp
  40% of sequence in fragments shorter than     966 bp
  45% of sequence in fragments shorter than    1092 bp
  50% of sequence in fragments shorter than    1229 bp
  55% of sequence in fragments shorter than    1378 bp
  60% of sequence in fragments shorter than    1542 bp
  65% of sequence in fragments shorter than    1714 bp
  70% of sequence in fragments shorter than    1920 bp
  75% of sequence in fragments shorter than    2149 bp
  80% of sequence in fragments shorter than    2447 bp
  85% of sequence in fragments shorter than    2825 bp
  90% of sequence in fragments shorter than    3353 bp
  95% of sequence in fragments shorter than    4283 bp

# BscAI.PsiI (GCATC+TTATAA) double digest, 5300 thousand fragments (3652926+1647912 sites), 562 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     295 bp
  15% of sequence in fragments shorter than     385 bp
  20% of sequence in fragments shorter than     471 bp
  25% of sequence in fragments shorter than     552 bp
  30% of sequence in fragments shorter than     633 bp
  35% of sequence in fragments shorter than     713 bp
  40% of sequence in fragments shorter than     797 bp
  45% of sequence in fragments shorter than     885 bp
  50% of sequence in fragments shorter than     978 bp
  55% of sequence in fragments shorter than    1081 bp
  60% of sequence in fragments shorter than    1191 bp
  65% of sequence in fragments shorter than    1313 bp
  70% of sequence in fragments shorter than    1449 bp
  75% of sequence in fragments shorter than    1608 bp
  80% of sequence in fragments shorter than    1804 bp
  85% of sequence in fragments shorter than    2053 bp
  90% of sequence in fragments shorter than    2413 bp
  95% of sequence in fragments shorter than    3066 bp

# BbvI.NotI (GCAGC+GCGGCCGC) double digest, 5299 thousand fragments (5290051+9795 sites), 562 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     301 bp
  15% of sequence in fragments shorter than     407 bp
  20% of sequence in fragments shorter than     516 bp
  25% of sequence in fragments shorter than     626 bp
  30% of sequence in fragments shorter than     736 bp
  35% of sequence in fragments shorter than     850 bp
  40% of sequence in fragments shorter than     967 bp
  45% of sequence in fragments shorter than    1093 bp
  50% of sequence in fragments shorter than    1230 bp
  55% of sequence in fragments shorter than    1378 bp
  60% of sequence in fragments shorter than    1544 bp
  65% of sequence in fragments shorter than    1715 bp
  70% of sequence in fragments shorter than    1921 bp
  75% of sequence in fragments shorter than    2151 bp
  80% of sequence in fragments shorter than    2449 bp
  85% of sequence in fragments shorter than    2827 bp
  90% of sequence in fragments shorter than    3355 bp
  95% of sequence in fragments shorter than    4287 bp

# BbvI.RsrII (GCAGC+CGGWCCG) double digest, 5299 thousand fragments (5290051+9764 sites), 562 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     301 bp
  15% of sequence in fragments shorter than     407 bp
  20% of sequence in fragments shorter than     516 bp
  25% of sequence in fragments shorter than     626 bp
  30% of sequence in fragments shorter than     736 bp
  35% of sequence in fragments shorter than     850 bp
  40% of sequence in fragments shorter than     966 bp
  45% of sequence in fragments shorter than    1093 bp
  50% of sequence in fragments shorter than    1229 bp
  55% of sequence in fragments shorter than    1378 bp
  60% of sequence in fragments shorter than    1543 bp
  65% of sequence in fragments shorter than    1714 bp
  70% of sequence in fragments shorter than    1920 bp
  75% of sequence in fragments shorter than    2150 bp
  80% of sequence in fragments shorter than    2448 bp
  85% of sequence in fragments shorter than    2826 bp
  90% of sequence in fragments shorter than    3354 bp
  95% of sequence in fragments shorter than    4285 bp

# AscI.BbvI (GGCGCGCC+GCAGC) double digest, 5294 thousand fragments (4751+5290051 sites), 562 bp average fragment length
   5% of sequence in fragments shorter than     186 bp
  10% of sequence in fragments shorter than     301 bp
  15% of sequence in fragments shorter than     407 bp
  20% of sequence in fragments shorter than     516 bp
  25% of sequence in fragments shorter than     626 bp
  30% of sequence in fragments shorter than     736 bp
  35% of sequence in fragments shorter than     850 bp
  40% of sequence in fragments shorter than     967 bp
  45% of sequence in fragments shorter than    1094 bp
  50% of sequence in fragments shorter than    1230 bp
  55% of sequence in fragments shorter than    1379 bp
  60% of sequence in fragments shorter than    1544 bp
  65% of sequence in fragments shorter than    1715 bp
  70% of sequence in fragments shorter than    1921 bp
  75% of sequence in fragments shorter than    2151 bp
  80% of sequence in fragments shorter than    2449 bp
  85% of sequence in fragments shorter than    2827 bp
  90% of sequence in fragments shorter than    3355 bp
  95% of sequence in fragments shorter than    4286 bp

# AciI.PpuMI (CCGC+RGGWCCY) double digest, 5290 thousand fragments (4206130+1085138 sites), 563 bp average fragment length
   5% of sequence in fragments shorter than     173 bp
  10% of sequence in fragments shorter than     320 bp
  15% of sequence in fragments shorter than     460 bp
  20% of sequence in fragments shorter than     601 bp
  25% of sequence in fragments shorter than     745 bp
  30% of sequence in fragments shorter than     889 bp
  35% of sequence in fragments shorter than    1043 bp
  40% of sequence in fragments shorter than    1210 bp
  45% of sequence in fragments shorter than    1380 bp
  50% of sequence in fragments shorter than    1569 bp
  55% of sequence in fragments shorter than    1779 bp
  60% of sequence in fragments shorter than    2016 bp
  65% of sequence in fragments shorter than    2284 bp
  70% of sequence in fragments shorter than    2594 bp
  75% of sequence in fragments shorter than    2962 bp
  80% of sequence in fragments shorter than    3414 bp
  85% of sequence in fragments shorter than    3991 bp
  90% of sequence in fragments shorter than    4828 bp
  95% of sequence in fragments shorter than    6286 bp

# AciI.PvuII (CCGC+CAGCTG) double digest, 5289 thousand fragments (4206130+1084260 sites), 563 bp average fragment length
   5% of sequence in fragments shorter than     174 bp
  10% of sequence in fragments shorter than     322 bp
  15% of sequence in fragments shorter than     460 bp
  20% of sequence in fragments shorter than     596 bp
  25% of sequence in fragments shorter than     736 bp
  30% of sequence in fragments shorter than     877 bp
  35% of sequence in fragments shorter than    1028 bp
  40% of sequence in fragments shorter than    1185 bp
  45% of sequence in fragments shorter than    1360 bp
  50% of sequence in fragments shorter than    1539 bp
  55% of sequence in fragments shorter than    1745 bp
  60% of sequence in fragments shorter than    1974 bp
  65% of sequence in fragments shorter than    2239 bp
  70% of sequence in fragments shorter than    2543 bp
  75% of sequence in fragments shorter than    2896 bp
  80% of sequence in fragments shorter than    3337 bp
  85% of sequence in fragments shorter than    3909 bp
  90% of sequence in fragments shorter than    4720 bp
  95% of sequence in fragments shorter than    6125 bp

# AclI.CviQI (AACGTT+GTAC) double digest, 5287 thousand fragments (172706+5115078 sites), 563 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     301 bp
  15% of sequence in fragments shorter than     392 bp
  20% of sequence in fragments shorter than     477 bp
  25% of sequence in fragments shorter than     556 bp
  30% of sequence in fragments shorter than     640 bp
  35% of sequence in fragments shorter than     721 bp
  40% of sequence in fragments shorter than     805 bp
  45% of sequence in fragments shorter than     895 bp
  50% of sequence in fragments shorter than     991 bp
  55% of sequence in fragments shorter than    1089 bp
  60% of sequence in fragments shorter than    1199 bp
  65% of sequence in fragments shorter than    1315 bp
  70% of sequence in fragments shorter than    1444 bp
  75% of sequence in fragments shorter than    1605 bp
  80% of sequence in fragments shorter than    1801 bp
  85% of sequence in fragments shorter than    2053 bp
  90% of sequence in fragments shorter than    2407 bp
  95% of sequence in fragments shorter than    3043 bp

# ClaI.FokI (ATCGAT+GGATG) double digest, 5287 thousand fragments (86477+5201116 sites), 563 bp average fragment length
   5% of sequence in fragments shorter than     195 bp
  10% of sequence in fragments shorter than     299 bp
  15% of sequence in fragments shorter than     389 bp
  20% of sequence in fragments shorter than     479 bp
  25% of sequence in fragments shorter than     567 bp
  30% of sequence in fragments shorter than     655 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     837 bp
  45% of sequence in fragments shorter than     930 bp
  50% of sequence in fragments shorter than    1033 bp
  55% of sequence in fragments shorter than    1139 bp
  60% of sequence in fragments shorter than    1255 bp
  65% of sequence in fragments shorter than    1384 bp
  70% of sequence in fragments shorter than    1530 bp
  75% of sequence in fragments shorter than    1705 bp
  80% of sequence in fragments shorter than    1912 bp
  85% of sequence in fragments shorter than    2186 bp
  90% of sequence in fragments shorter than    2571 bp
  95% of sequence in fragments shorter than    3262 bp

# HhaI.SfeI (GCGC+CTRYAG) double digest, 5287 thousand fragments (1756502+3531009 sites), 563 bp average fragment length
   5% of sequence in fragments shorter than     189 bp
  10% of sequence in fragments shorter than     308 bp
  15% of sequence in fragments shorter than     402 bp
  20% of sequence in fragments shorter than     497 bp
  25% of sequence in fragments shorter than     592 bp
  30% of sequence in fragments shorter than     686 bp
  35% of sequence in fragments shorter than     782 bp
  40% of sequence in fragments shorter than     884 bp
  45% of sequence in fragments shorter than     993 bp
  50% of sequence in fragments shorter than    1103 bp
  55% of sequence in fragments shorter than    1228 bp
  60% of sequence in fragments shorter than    1361 bp
  65% of sequence in fragments shorter than    1513 bp
  70% of sequence in fragments shorter than    1684 bp
  75% of sequence in fragments shorter than    1881 bp
  80% of sequence in fragments shorter than    2120 bp
  85% of sequence in fragments shorter than    2401 bp
  90% of sequence in fragments shorter than    2822 bp
  95% of sequence in fragments shorter than    3541 bp

# SexAI.TfiI (ACCWGGT+GAWTC) double digest, 5281 thousand fragments (297900+4984362 sites), 564 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     299 bp
  15% of sequence in fragments shorter than     367 bp
  20% of sequence in fragments shorter than     453 bp
  25% of sequence in fragments shorter than     537 bp
  30% of sequence in fragments shorter than     623 bp
  35% of sequence in fragments shorter than     703 bp
  40% of sequence in fragments shorter than     789 bp
  45% of sequence in fragments shorter than     876 bp
  50% of sequence in fragments shorter than     973 bp
  55% of sequence in fragments shorter than    1073 bp
  60% of sequence in fragments shorter than    1184 bp
  65% of sequence in fragments shorter than    1306 bp
  70% of sequence in fragments shorter than    1444 bp
  75% of sequence in fragments shorter than    1604 bp
  80% of sequence in fragments shorter than    1795 bp
  85% of sequence in fragments shorter than    2031 bp
  90% of sequence in fragments shorter than    2360 bp
  95% of sequence in fragments shorter than    2912 bp

# CviQI.PacI (GTAC+TTAATTAA) double digest, 5274 thousand fragments (5115078+159455 sites), 565 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     394 bp
  20% of sequence in fragments shorter than     478 bp
  25% of sequence in fragments shorter than     558 bp
  30% of sequence in fragments shorter than     642 bp
  35% of sequence in fragments shorter than     724 bp
  40% of sequence in fragments shorter than     809 bp
  45% of sequence in fragments shorter than     898 bp
  50% of sequence in fragments shorter than     996 bp
  55% of sequence in fragments shorter than    1097 bp
  60% of sequence in fragments shorter than    1209 bp
  65% of sequence in fragments shorter than    1327 bp
  70% of sequence in fragments shorter than    1455 bp
  75% of sequence in fragments shorter than    1617 bp
  80% of sequence in fragments shorter than    1816 bp
  85% of sequence in fragments shorter than    2071 bp
  90% of sequence in fragments shorter than    2434 bp
  95% of sequence in fragments shorter than    3092 bp

# CviQI.Hpy99I (GTAC+CGWCG) double digest, 5272 thousand fragments (5115078+157917 sites), 565 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     479 bp
  25% of sequence in fragments shorter than     558 bp
  30% of sequence in fragments shorter than     642 bp
  35% of sequence in fragments shorter than     724 bp
  40% of sequence in fragments shorter than     808 bp
  45% of sequence in fragments shorter than     897 bp
  50% of sequence in fragments shorter than     994 bp
  55% of sequence in fragments shorter than    1094 bp
  60% of sequence in fragments shorter than    1205 bp
  65% of sequence in fragments shorter than    1321 bp
  70% of sequence in fragments shorter than    1446 bp
  75% of sequence in fragments shorter than    1606 bp
  80% of sequence in fragments shorter than    1798 bp
  85% of sequence in fragments shorter than    2048 bp
  90% of sequence in fragments shorter than    2396 bp
  95% of sequence in fragments shorter than    3023 bp

# KpnI.TfiI (GGTACC+GAWTC) double digest, 5271 thousand fragments (287974+4984362 sites), 565 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     298 bp
  15% of sequence in fragments shorter than     368 bp
  20% of sequence in fragments shorter than     456 bp
  25% of sequence in fragments shorter than     539 bp
  30% of sequence in fragments shorter than     626 bp
  35% of sequence in fragments shorter than     706 bp
  40% of sequence in fragments shorter than     793 bp
  45% of sequence in fragments shorter than     883 bp
  50% of sequence in fragments shorter than     980 bp
  55% of sequence in fragments shorter than    1082 bp
  60% of sequence in fragments shorter than    1194 bp
  65% of sequence in fragments shorter than    1317 bp
  70% of sequence in fragments shorter than    1454 bp
  75% of sequence in fragments shorter than    1615 bp
  80% of sequence in fragments shorter than    1806 bp
  85% of sequence in fragments shorter than    2046 bp
  90% of sequence in fragments shorter than    2381 bp
  95% of sequence in fragments shorter than    2940 bp

# AatII.FokI (GACGTC+GGATG) double digest, 5271 thousand fragments (71089+5201116 sites), 565 bp average fragment length
   5% of sequence in fragments shorter than     195 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     390 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     569 bp
  30% of sequence in fragments shorter than     657 bp
  35% of sequence in fragments shorter than     747 bp
  40% of sequence in fragments shorter than     841 bp
  45% of sequence in fragments shorter than     935 bp
  50% of sequence in fragments shorter than    1038 bp
  55% of sequence in fragments shorter than    1147 bp
  60% of sequence in fragments shorter than    1264 bp
  65% of sequence in fragments shorter than    1390 bp
  70% of sequence in fragments shorter than    1540 bp
  75% of sequence in fragments shorter than    1717 bp
  80% of sequence in fragments shorter than    1926 bp
  85% of sequence in fragments shorter than    2204 bp
  90% of sequence in fragments shorter than    2593 bp
  95% of sequence in fragments shorter than    3294 bp

# NheI.TfiI (GCTAGC+GAWTC) double digest, 5271 thousand fragments (287384+4984362 sites), 565 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     302 bp
  15% of sequence in fragments shorter than     366 bp
  20% of sequence in fragments shorter than     456 bp
  25% of sequence in fragments shorter than     541 bp
  30% of sequence in fragments shorter than     628 bp
  35% of sequence in fragments shorter than     708 bp
  40% of sequence in fragments shorter than     796 bp
  45% of sequence in fragments shorter than     885 bp
  50% of sequence in fragments shorter than     982 bp
  55% of sequence in fragments shorter than    1084 bp
  60% of sequence in fragments shorter than    1196 bp
  65% of sequence in fragments shorter than    1318 bp
  70% of sequence in fragments shorter than    1457 bp
  75% of sequence in fragments shorter than    1619 bp
  80% of sequence in fragments shorter than    1813 bp
  85% of sequence in fragments shorter than    2055 bp
  90% of sequence in fragments shorter than    2392 bp
  95% of sequence in fragments shorter than    2957 bp

# FokI.SacII (GGATG+CCGCGG) double digest, 5267 thousand fragments (5201116+66901 sites), 565 bp average fragment length
   5% of sequence in fragments shorter than     195 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     390 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     658 bp
  35% of sequence in fragments shorter than     749 bp
  40% of sequence in fragments shorter than     843 bp
  45% of sequence in fragments shorter than     938 bp
  50% of sequence in fragments shorter than    1042 bp
  55% of sequence in fragments shorter than    1151 bp
  60% of sequence in fragments shorter than    1269 bp
  65% of sequence in fragments shorter than    1395 bp
  70% of sequence in fragments shorter than    1547 bp
  75% of sequence in fragments shorter than    1723 bp
  80% of sequence in fragments shorter than    1937 bp
  85% of sequence in fragments shorter than    2217 bp
  90% of sequence in fragments shorter than    2610 bp
  95% of sequence in fragments shorter than    3316 bp

# FokI.PspXI (GGATG+VCTCGAGB) double digest, 5259 thousand fragments (5201116+59193 sites), 566 bp average fragment length
   5% of sequence in fragments shorter than     195 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     391 bp
  20% of sequence in fragments shorter than     482 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     658 bp
  35% of sequence in fragments shorter than     748 bp
  40% of sequence in fragments shorter than     842 bp
  45% of sequence in fragments shorter than     936 bp
  50% of sequence in fragments shorter than    1040 bp
  55% of sequence in fragments shorter than    1149 bp
  60% of sequence in fragments shorter than    1266 bp
  65% of sequence in fragments shorter than    1392 bp
  70% of sequence in fragments shorter than    1543 bp
  75% of sequence in fragments shorter than    1718 bp
  80% of sequence in fragments shorter than    1930 bp
  85% of sequence in fragments shorter than    2208 bp
  90% of sequence in fragments shorter than    2598 bp
  95% of sequence in fragments shorter than    3299 bp

# BscAI.EsaBC3I (GCATC+TCGA) double digest, 5255 thousand fragments (3652926+1603346 sites), 566 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     389 bp
  20% of sequence in fragments shorter than     472 bp
  25% of sequence in fragments shorter than     555 bp
  30% of sequence in fragments shorter than     636 bp
  35% of sequence in fragments shorter than     720 bp
  40% of sequence in fragments shorter than     806 bp
  45% of sequence in fragments shorter than     898 bp
  50% of sequence in fragments shorter than     996 bp
  55% of sequence in fragments shorter than    1098 bp
  60% of sequence in fragments shorter than    1212 bp
  65% of sequence in fragments shorter than    1338 bp
  70% of sequence in fragments shorter than    1479 bp
  75% of sequence in fragments shorter than    1641 bp
  80% of sequence in fragments shorter than    1841 bp
  85% of sequence in fragments shorter than    2094 bp
  90% of sequence in fragments shorter than    2451 bp
  95% of sequence in fragments shorter than    3058 bp

# AgeI.FokI (ACCGGT+GGATG) double digest, 5254 thousand fragments (53386+5201116 sites), 567 bp average fragment length
   5% of sequence in fragments shorter than     195 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     391 bp
  20% of sequence in fragments shorter than     482 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     659 bp
  35% of sequence in fragments shorter than     749 bp
  40% of sequence in fragments shorter than     843 bp
  45% of sequence in fragments shorter than     936 bp
  50% of sequence in fragments shorter than    1040 bp
  55% of sequence in fragments shorter than    1148 bp
  60% of sequence in fragments shorter than    1267 bp
  65% of sequence in fragments shorter than    1393 bp
  70% of sequence in fragments shorter than    1545 bp
  75% of sequence in fragments shorter than    1719 bp
  80% of sequence in fragments shorter than    1931 bp
  85% of sequence in fragments shorter than    2209 bp
  90% of sequence in fragments shorter than    2599 bp
  95% of sequence in fragments shorter than    3301 bp

# MlyI.PstI (GAGTC+CTGCAG) double digest, 5252 thousand fragments (3931692+1321469 sites), 567 bp average fragment length
   5% of sequence in fragments shorter than     194 bp
  10% of sequence in fragments shorter than     298 bp
  15% of sequence in fragments shorter than     383 bp
  20% of sequence in fragments shorter than     467 bp
  25% of sequence in fragments shorter than     550 bp
  30% of sequence in fragments shorter than     635 bp
  35% of sequence in fragments shorter than     717 bp
  40% of sequence in fragments shorter than     808 bp
  45% of sequence in fragments shorter than     903 bp
  50% of sequence in fragments shorter than    1006 bp
  55% of sequence in fragments shorter than    1117 bp
  60% of sequence in fragments shorter than    1242 bp
  65% of sequence in fragments shorter than    1379 bp
  70% of sequence in fragments shorter than    1539 bp
  75% of sequence in fragments shorter than    1731 bp
  80% of sequence in fragments shorter than    1971 bp
  85% of sequence in fragments shorter than    2278 bp
  90% of sequence in fragments shorter than    2739 bp
  95% of sequence in fragments shorter than    3574 bp

# BscGI.Tsp45I (CCCGT+GTSAC) double digest, 5252 thousand fragments (1231070+4021761 sites), 567 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     293 bp
  15% of sequence in fragments shorter than     374 bp
  20% of sequence in fragments shorter than     462 bp
  25% of sequence in fragments shorter than     548 bp
  30% of sequence in fragments shorter than     634 bp
  35% of sequence in fragments shorter than     722 bp
  40% of sequence in fragments shorter than     816 bp
  45% of sequence in fragments shorter than     915 bp
  50% of sequence in fragments shorter than    1020 bp
  55% of sequence in fragments shorter than    1136 bp
  60% of sequence in fragments shorter than    1263 bp
  65% of sequence in fragments shorter than    1405 bp
  70% of sequence in fragments shorter than    1570 bp
  75% of sequence in fragments shorter than    1763 bp
  80% of sequence in fragments shorter than    2000 bp
  85% of sequence in fragments shorter than    2311 bp
  90% of sequence in fragments shorter than    2758 bp
  95% of sequence in fragments shorter than    3563 bp

# CviQI.SnaBI (GTAC+TACGTA) double digest, 5246 thousand fragments (5115078+132299 sites), 567 bp average fragment length
   5% of sequence in fragments shorter than     199 bp
  10% of sequence in fragments shorter than     306 bp
  15% of sequence in fragments shorter than     399 bp
  20% of sequence in fragments shorter than     484 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     650 bp
  35% of sequence in fragments shorter than     733 bp
  40% of sequence in fragments shorter than     819 bp
  45% of sequence in fragments shorter than     910 bp
  50% of sequence in fragments shorter than    1008 bp
  55% of sequence in fragments shorter than    1110 bp
  60% of sequence in fragments shorter than    1220 bp
  65% of sequence in fragments shorter than    1342 bp
  70% of sequence in fragments shorter than    1472 bp
  75% of sequence in fragments shorter than    1636 bp
  80% of sequence in fragments shorter than    1837 bp
  85% of sequence in fragments shorter than    2092 bp
  90% of sequence in fragments shorter than    2460 bp
  95% of sequence in fragments shorter than    3121 bp

# FokI.PmeI (GGATG+GTTTAAAC) double digest, 5242 thousand fragments (5201116+42094 sites), 568 bp average fragment length
   5% of sequence in fragments shorter than     196 bp
  10% of sequence in fragments shorter than     302 bp
  15% of sequence in fragments shorter than     393 bp
  20% of sequence in fragments shorter than     483 bp
  25% of sequence in fragments shorter than     572 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     750 bp
  40% of sequence in fragments shorter than     844 bp
  45% of sequence in fragments shorter than     938 bp
  50% of sequence in fragments shorter than    1041 bp
  55% of sequence in fragments shorter than    1150 bp
  60% of sequence in fragments shorter than    1267 bp
  65% of sequence in fragments shorter than    1392 bp
  70% of sequence in fragments shorter than    1543 bp
  75% of sequence in fragments shorter than    1717 bp
  80% of sequence in fragments shorter than    1927 bp
  85% of sequence in fragments shorter than    2203 bp
  90% of sequence in fragments shorter than    2589 bp
  95% of sequence in fragments shorter than    3284 bp

# CviQI.NaeI (GTAC+GCCGGC) double digest, 5240 thousand fragments (5115078+125692 sites), 568 bp average fragment length
   5% of sequence in fragments shorter than     199 bp
  10% of sequence in fragments shorter than     305 bp
  15% of sequence in fragments shorter than     397 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     561 bp
  30% of sequence in fragments shorter than     645 bp
  35% of sequence in fragments shorter than     727 bp
  40% of sequence in fragments shorter than     811 bp
  45% of sequence in fragments shorter than     900 bp
  50% of sequence in fragments shorter than     997 bp
  55% of sequence in fragments shorter than    1097 bp
  60% of sequence in fragments shorter than    1208 bp
  65% of sequence in fragments shorter than    1325 bp
  70% of sequence in fragments shorter than    1450 bp
  75% of sequence in fragments shorter than    1610 bp
  80% of sequence in fragments shorter than    1804 bp
  85% of sequence in fragments shorter than    2054 bp
  90% of sequence in fragments shorter than    2402 bp
  95% of sequence in fragments shorter than    3027 bp

# BfiI.SfeI (ACTGGG+CTRYAG) double digest, 5237 thousand fragments (1706594+3531009 sites), 569 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     300 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     466 bp
  25% of sequence in fragments shorter than     549 bp
  30% of sequence in fragments shorter than     633 bp
  35% of sequence in fragments shorter than     713 bp
  40% of sequence in fragments shorter than     801 bp
  45% of sequence in fragments shorter than     892 bp
  50% of sequence in fragments shorter than     988 bp
  55% of sequence in fragments shorter than    1090 bp
  60% of sequence in fragments shorter than    1202 bp
  65% of sequence in fragments shorter than    1331 bp
  70% of sequence in fragments shorter than    1469 bp
  75% of sequence in fragments shorter than    1636 bp
  80% of sequence in fragments shorter than    1836 bp
  85% of sequence in fragments shorter than    2068 bp
  90% of sequence in fragments shorter than    2414 bp
  95% of sequence in fragments shorter than    3021 bp

# CviQI.XhoI (GTAC+CTCGAG) double digest, 5235 thousand fragments (5115078+121308 sites), 569 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     305 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     480 bp
  25% of sequence in fragments shorter than     562 bp
  30% of sequence in fragments shorter than     646 bp
  35% of sequence in fragments shorter than     728 bp
  40% of sequence in fragments shorter than     813 bp
  45% of sequence in fragments shorter than     902 bp
  50% of sequence in fragments shorter than     999 bp
  55% of sequence in fragments shorter than    1100 bp
  60% of sequence in fragments shorter than    1211 bp
  65% of sequence in fragments shorter than    1329 bp
  70% of sequence in fragments shorter than    1455 bp
  75% of sequence in fragments shorter than    1616 bp
  80% of sequence in fragments shorter than    1813 bp
  85% of sequence in fragments shorter than    2065 bp
  90% of sequence in fragments shorter than    2419 bp
  95% of sequence in fragments shorter than    3058 bp

# FokI.SalI (GGATG+GTCGAC) double digest, 5232 thousand fragments (5201116+31396 sites), 569 bp average fragment length
   5% of sequence in fragments shorter than     196 bp
  10% of sequence in fragments shorter than     302 bp
  15% of sequence in fragments shorter than     393 bp
  20% of sequence in fragments shorter than     484 bp
  25% of sequence in fragments shorter than     573 bp
  30% of sequence in fragments shorter than     662 bp
  35% of sequence in fragments shorter than     753 bp
  40% of sequence in fragments shorter than     847 bp
  45% of sequence in fragments shorter than     941 bp
  50% of sequence in fragments shorter than    1045 bp
  55% of sequence in fragments shorter than    1154 bp
  60% of sequence in fragments shorter than    1272 bp
  65% of sequence in fragments shorter than    1398 bp
  70% of sequence in fragments shorter than    1548 bp
  75% of sequence in fragments shorter than    1724 bp
  80% of sequence in fragments shorter than    1938 bp
  85% of sequence in fragments shorter than    2217 bp
  90% of sequence in fragments shorter than    2609 bp
  95% of sequence in fragments shorter than    3311 bp

# FokI.MluI (GGATG+ACGCGT) double digest, 5222 thousand fragments (5201116+21415 sites), 570 bp average fragment length
   5% of sequence in fragments shorter than     196 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     394 bp
  20% of sequence in fragments shorter than     485 bp
  25% of sequence in fragments shorter than     574 bp
  30% of sequence in fragments shorter than     664 bp
  35% of sequence in fragments shorter than     754 bp
  40% of sequence in fragments shorter than     849 bp
  45% of sequence in fragments shorter than     943 bp
  50% of sequence in fragments shorter than    1047 bp
  55% of sequence in fragments shorter than    1157 bp
  60% of sequence in fragments shorter than    1275 bp
  65% of sequence in fragments shorter than    1401 bp
  70% of sequence in fragments shorter than    1551 bp
  75% of sequence in fragments shorter than    1727 bp
  80% of sequence in fragments shorter than    1942 bp
  85% of sequence in fragments shorter than    2222 bp
  90% of sequence in fragments shorter than    2615 bp
  95% of sequence in fragments shorter than    3320 bp

# BsrBI.TfiI (CCGCTC+GAWTC) double digest, 5219 thousand fragments (235523+4984362 sites), 570 bp average fragment length
   5% of sequence in fragments shorter than     196 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     372 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     545 bp
  30% of sequence in fragments shorter than     631 bp
  35% of sequence in fragments shorter than     712 bp
  40% of sequence in fragments shorter than     799 bp
  45% of sequence in fragments shorter than     887 bp
  50% of sequence in fragments shorter than     985 bp
  55% of sequence in fragments shorter than    1087 bp
  60% of sequence in fragments shorter than    1199 bp
  65% of sequence in fragments shorter than    1322 bp
  70% of sequence in fragments shorter than    1461 bp
  75% of sequence in fragments shorter than    1622 bp
  80% of sequence in fragments shorter than    1813 bp
  85% of sequence in fragments shorter than    2049 bp
  90% of sequence in fragments shorter than    2378 bp
  95% of sequence in fragments shorter than    2923 bp

# FokI.SgrAI (GGATG+CRCCGGYG) double digest, 5215 thousand fragments (5201116+15270 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     486 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     664 bp
  35% of sequence in fragments shorter than     755 bp
  40% of sequence in fragments shorter than     850 bp
  45% of sequence in fragments shorter than     944 bp
  50% of sequence in fragments shorter than    1048 bp
  55% of sequence in fragments shorter than    1158 bp
  60% of sequence in fragments shorter than    1276 bp
  65% of sequence in fragments shorter than    1402 bp
  70% of sequence in fragments shorter than    1553 bp
  75% of sequence in fragments shorter than    1730 bp
  80% of sequence in fragments shorter than    1945 bp
  85% of sequence in fragments shorter than    2226 bp
  90% of sequence in fragments shorter than    2619 bp
  95% of sequence in fragments shorter than    3324 bp

# FokI.NruI (GGATG+TCGCGA) double digest, 5215 thousand fragments (5201116+14872 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     486 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     664 bp
  35% of sequence in fragments shorter than     755 bp
  40% of sequence in fragments shorter than     850 bp
  45% of sequence in fragments shorter than     944 bp
  50% of sequence in fragments shorter than    1048 bp
  55% of sequence in fragments shorter than    1158 bp
  60% of sequence in fragments shorter than    1276 bp
  65% of sequence in fragments shorter than    1402 bp
  70% of sequence in fragments shorter than    1553 bp
  75% of sequence in fragments shorter than    1729 bp
  80% of sequence in fragments shorter than    1944 bp
  85% of sequence in fragments shorter than    2225 bp
  90% of sequence in fragments shorter than    2617 bp
  95% of sequence in fragments shorter than    3320 bp

# FokI.PvuI (GGATG+CGATCG) double digest, 5214 thousand fragments (5201116+14186 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     486 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     665 bp
  35% of sequence in fragments shorter than     755 bp
  40% of sequence in fragments shorter than     850 bp
  45% of sequence in fragments shorter than     944 bp
  50% of sequence in fragments shorter than    1048 bp
  55% of sequence in fragments shorter than    1157 bp
  60% of sequence in fragments shorter than    1276 bp
  65% of sequence in fragments shorter than    1402 bp
  70% of sequence in fragments shorter than    1553 bp
  75% of sequence in fragments shorter than    1730 bp
  80% of sequence in fragments shorter than    1945 bp
  85% of sequence in fragments shorter than    2225 bp
  90% of sequence in fragments shorter than    2618 bp
  95% of sequence in fragments shorter than    3323 bp

# FokI.FseI (GGATG+GGCCGGCC) double digest, 5214 thousand fragments (5201116+13662 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     486 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     665 bp
  35% of sequence in fragments shorter than     756 bp
  40% of sequence in fragments shorter than     850 bp
  45% of sequence in fragments shorter than     945 bp
  50% of sequence in fragments shorter than    1048 bp
  55% of sequence in fragments shorter than    1158 bp
  60% of sequence in fragments shorter than    1277 bp
  65% of sequence in fragments shorter than    1403 bp
  70% of sequence in fragments shorter than    1554 bp
  75% of sequence in fragments shorter than    1730 bp
  80% of sequence in fragments shorter than    1946 bp
  85% of sequence in fragments shorter than    2227 bp
  90% of sequence in fragments shorter than    2620 bp
  95% of sequence in fragments shorter than    3327 bp

# KasI.TfiI (GGCGCC+GAWTC) double digest, 5214 thousand fragments (230381+4984362 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     304 bp
  15% of sequence in fragments shorter than     372 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     544 bp
  30% of sequence in fragments shorter than     630 bp
  35% of sequence in fragments shorter than     710 bp
  40% of sequence in fragments shorter than     797 bp
  45% of sequence in fragments shorter than     885 bp
  50% of sequence in fragments shorter than     981 bp
  55% of sequence in fragments shorter than    1081 bp
  60% of sequence in fragments shorter than    1192 bp
  65% of sequence in fragments shorter than    1314 bp
  70% of sequence in fragments shorter than    1453 bp
  75% of sequence in fragments shorter than    1613 bp
  80% of sequence in fragments shorter than    1804 bp
  85% of sequence in fragments shorter than    2041 bp
  90% of sequence in fragments shorter than    2371 bp
  95% of sequence in fragments shorter than    2923 bp

# AciI.AvaI (CCGC+CYCGRG) double digest, 5211 thousand fragments (4206130+1005714 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     170 bp
  10% of sequence in fragments shorter than     341 bp
  15% of sequence in fragments shorter than     502 bp
  20% of sequence in fragments shorter than     667 bp
  25% of sequence in fragments shorter than     836 bp
  30% of sequence in fragments shorter than    1007 bp
  35% of sequence in fragments shorter than    1192 bp
  40% of sequence in fragments shorter than    1387 bp
  45% of sequence in fragments shorter than    1605 bp
  50% of sequence in fragments shorter than    1849 bp
  55% of sequence in fragments shorter than    2114 bp
  60% of sequence in fragments shorter than    2416 bp
  65% of sequence in fragments shorter than    2757 bp
  70% of sequence in fragments shorter than    3154 bp
  75% of sequence in fragments shorter than    3630 bp
  80% of sequence in fragments shorter than    4214 bp
  85% of sequence in fragments shorter than    4965 bp
  90% of sequence in fragments shorter than    6042 bp
  95% of sequence in fragments shorter than    7896 bp

# SwaI.TfiI (ATTTAAAT+GAWTC) double digest, 5210 thousand fragments (226602+4984362 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     196 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     372 bp
  20% of sequence in fragments shorter than     459 bp
  25% of sequence in fragments shorter than     544 bp
  30% of sequence in fragments shorter than     631 bp
  35% of sequence in fragments shorter than     711 bp
  40% of sequence in fragments shorter than     798 bp
  45% of sequence in fragments shorter than     887 bp
  50% of sequence in fragments shorter than     985 bp
  55% of sequence in fragments shorter than    1088 bp
  60% of sequence in fragments shorter than    1200 bp
  65% of sequence in fragments shorter than    1325 bp
  70% of sequence in fragments shorter than    1466 bp
  75% of sequence in fragments shorter than    1630 bp
  80% of sequence in fragments shorter than    1824 bp
  85% of sequence in fragments shorter than    2067 bp
  90% of sequence in fragments shorter than    2407 bp
  95% of sequence in fragments shorter than    2982 bp

# FokI.NotI (GGATG+GCGGCCGC) double digest, 5210 thousand fragments (5201116+9795 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     486 bp
  25% of sequence in fragments shorter than     576 bp
  30% of sequence in fragments shorter than     665 bp
  35% of sequence in fragments shorter than     756 bp
  40% of sequence in fragments shorter than     851 bp
  45% of sequence in fragments shorter than     945 bp
  50% of sequence in fragments shorter than    1049 bp
  55% of sequence in fragments shorter than    1159 bp
  60% of sequence in fragments shorter than    1278 bp
  65% of sequence in fragments shorter than    1404 bp
  70% of sequence in fragments shorter than    1555 bp
  75% of sequence in fragments shorter than    1731 bp
  80% of sequence in fragments shorter than    1947 bp
  85% of sequence in fragments shorter than    2228 bp
  90% of sequence in fragments shorter than    2621 bp
  95% of sequence in fragments shorter than    3328 bp

# FokI.RsrII (GGATG+CGGWCCG) double digest, 5210 thousand fragments (5201116+9764 sites), 571 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     487 bp
  25% of sequence in fragments shorter than     576 bp
  30% of sequence in fragments shorter than     665 bp
  35% of sequence in fragments shorter than     756 bp
  40% of sequence in fragments shorter than     851 bp
  45% of sequence in fragments shorter than     945 bp
  50% of sequence in fragments shorter than    1049 bp
  55% of sequence in fragments shorter than    1159 bp
  60% of sequence in fragments shorter than    1278 bp
  65% of sequence in fragments shorter than    1404 bp
  70% of sequence in fragments shorter than    1555 bp
  75% of sequence in fragments shorter than    1731 bp
  80% of sequence in fragments shorter than    1947 bp
  85% of sequence in fragments shorter than    2227 bp
  90% of sequence in fragments shorter than    2621 bp
  95% of sequence in fragments shorter than    3328 bp

# AscI.FokI (GGCGCGCC+GGATG) double digest, 5205 thousand fragments (4751+5201116 sites), 572 bp average fragment length
   5% of sequence in fragments shorter than     197 bp
  10% of sequence in fragments shorter than     304 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     487 bp
  25% of sequence in fragments shorter than     576 bp
  30% of sequence in fragments shorter than     666 bp
  35% of sequence in fragments shorter than     757 bp
  40% of sequence in fragments shorter than     851 bp
  45% of sequence in fragments shorter than     946 bp
  50% of sequence in fragments shorter than    1049 bp
  55% of sequence in fragments shorter than    1160 bp
  60% of sequence in fragments shorter than    1279 bp
  65% of sequence in fragments shorter than    1405 bp
  70% of sequence in fragments shorter than    1556 bp
  75% of sequence in fragments shorter than    1732 bp
  80% of sequence in fragments shorter than    1948 bp
  85% of sequence in fragments shorter than    2229 bp
  90% of sequence in fragments shorter than    2622 bp
  95% of sequence in fragments shorter than    3329 bp

# BscAI.HgiCI (GCATC+GGYRCC) double digest, 5203 thousand fragments (3652926+1550877 sites), 572 bp average fragment length
   5% of sequence in fragments shorter than     192 bp
  10% of sequence in fragments shorter than     296 bp
  15% of sequence in fragments shorter than     387 bp
  20% of sequence in fragments shorter than     477 bp
  25% of sequence in fragments shorter than     563 bp
  30% of sequence in fragments shorter than     646 bp
  35% of sequence in fragments shorter than     733 bp
  40% of sequence in fragments shorter than     825 bp
  45% of sequence in fragments shorter than     923 bp
  50% of sequence in fragments shorter than    1030 bp
  55% of sequence in fragments shorter than    1142 bp
  60% of sequence in fragments shorter than    1263 bp
  65% of sequence in fragments shorter than    1395 bp
  70% of sequence in fragments shorter than    1545 bp
  75% of sequence in fragments shorter than    1724 bp
  80% of sequence in fragments shorter than    1945 bp
  85% of sequence in fragments shorter than    2230 bp
  90% of sequence in fragments shorter than    2637 bp
  95% of sequence in fragments shorter than    3384 bp

# BscAI.BsmI (GCATC+GAATGC) double digest, 5201 thousand fragments (3652926+1549350 sites), 572 bp average fragment length
   5% of sequence in fragments shorter than     180 bp
  10% of sequence in fragments shorter than     295 bp
  15% of sequence in fragments shorter than     390 bp
  20% of sequence in fragments shorter than     486 bp
  25% of sequence in fragments shorter than     578 bp
  30% of sequence in fragments shorter than     672 bp
  35% of sequence in fragments shorter than     765 bp
  40% of sequence in fragments shorter than     860 bp
  45% of sequence in fragments shorter than     961 bp
  50% of sequence in fragments shorter than    1068 bp
  55% of sequence in fragments shorter than    1183 bp
  60% of sequence in fragments shorter than    1307 bp
  65% of sequence in fragments shorter than    1444 bp
  70% of sequence in fragments shorter than    1597 bp
  75% of sequence in fragments shorter than    1772 bp
  80% of sequence in fragments shorter than    1985 bp
  85% of sequence in fragments shorter than    2252 bp
  90% of sequence in fragments shorter than    2629 bp
  95% of sequence in fragments shorter than    3263 bp

# ClaI.CviQI (ATCGAT+GTAC) double digest, 5201 thousand fragments (86477+5115078 sites), 572 bp average fragment length
   5% of sequence in fragments shorter than     200 bp
  10% of sequence in fragments shorter than     307 bp
  15% of sequence in fragments shorter than     399 bp
  20% of sequence in fragments shorter than     484 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     650 bp
  35% of sequence in fragments shorter than     733 bp
  40% of sequence in fragments shorter than     819 bp
  45% of sequence in fragments shorter than     910 bp
  50% of sequence in fragments shorter than    1008 bp
  55% of sequence in fragments shorter than    1109 bp
  60% of sequence in fragments shorter than    1220 bp
  65% of sequence in fragments shorter than    1342 bp
  70% of sequence in fragments shorter than    1471 bp
  75% of sequence in fragments shorter than    1635 bp
  80% of sequence in fragments shorter than    1836 bp
  85% of sequence in fragments shorter than    2092 bp
  90% of sequence in fragments shorter than    2456 bp
  95% of sequence in fragments shorter than    3109 bp

# AatII.CviQI (GACGTC+GTAC) double digest, 5185 thousand fragments (71089+5115078 sites), 574 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     308 bp
  15% of sequence in fragments shorter than     401 bp
  20% of sequence in fragments shorter than     486 bp
  25% of sequence in fragments shorter than     567 bp
  30% of sequence in fragments shorter than     652 bp
  35% of sequence in fragments shorter than     735 bp
  40% of sequence in fragments shorter than     821 bp
  45% of sequence in fragments shorter than     911 bp
  50% of sequence in fragments shorter than    1009 bp
  55% of sequence in fragments shorter than    1112 bp
  60% of sequence in fragments shorter than    1221 bp
  65% of sequence in fragments shorter than    1343 bp
  70% of sequence in fragments shorter than    1471 bp
  75% of sequence in fragments shorter than    1634 bp
  80% of sequence in fragments shorter than    1834 bp
  85% of sequence in fragments shorter than    2087 bp
  90% of sequence in fragments shorter than    2447 bp
  95% of sequence in fragments shorter than    3094 bp

# AciI.BspHI (CCGC+TCATGA) double digest, 5183 thousand fragments (4206130+978290 sites), 574 bp average fragment length
   5% of sequence in fragments shorter than     187 bp
  10% of sequence in fragments shorter than     340 bp
  15% of sequence in fragments shorter than     475 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     735 bp
  30% of sequence in fragments shorter than     865 bp
  35% of sequence in fragments shorter than    1002 bp
  40% of sequence in fragments shorter than    1139 bp
  45% of sequence in fragments shorter than    1286 bp
  50% of sequence in fragments shorter than    1444 bp
  55% of sequence in fragments shorter than    1616 bp
  60% of sequence in fragments shorter than    1809 bp
  65% of sequence in fragments shorter than    2022 bp
  70% of sequence in fragments shorter than    2266 bp
  75% of sequence in fragments shorter than    2549 bp
  80% of sequence in fragments shorter than    2896 bp
  85% of sequence in fragments shorter than    3344 bp
  90% of sequence in fragments shorter than    3972 bp
  95% of sequence in fragments shorter than    5057 bp

# CviQI.SacII (GTAC+CCGCGG) double digest, 5181 thousand fragments (5115078+66901 sites), 575 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     308 bp
  15% of sequence in fragments shorter than     401 bp
  20% of sequence in fragments shorter than     487 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     653 bp
  35% of sequence in fragments shorter than     737 bp
  40% of sequence in fragments shorter than     823 bp
  45% of sequence in fragments shorter than     913 bp
  50% of sequence in fragments shorter than    1011 bp
  55% of sequence in fragments shorter than    1114 bp
  60% of sequence in fragments shorter than    1223 bp
  65% of sequence in fragments shorter than    1345 bp
  70% of sequence in fragments shorter than    1473 bp
  75% of sequence in fragments shorter than    1636 bp
  80% of sequence in fragments shorter than    1836 bp
  85% of sequence in fragments shorter than    2088 bp
  90% of sequence in fragments shorter than    2448 bp
  95% of sequence in fragments shorter than    3096 bp

# PsiI.SfeI (TTATAA+CTRYAG) double digest, 5178 thousand fragments (1647912+3531009 sites), 575 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     303 bp
  15% of sequence in fragments shorter than     390 bp
  20% of sequence in fragments shorter than     468 bp
  25% of sequence in fragments shorter than     547 bp
  30% of sequence in fragments shorter than     628 bp
  35% of sequence in fragments shorter than     706 bp
  40% of sequence in fragments shorter than     790 bp
  45% of sequence in fragments shorter than     879 bp
  50% of sequence in fragments shorter than     971 bp
  55% of sequence in fragments shorter than    1070 bp
  60% of sequence in fragments shorter than    1176 bp
  65% of sequence in fragments shorter than    1296 bp
  70% of sequence in fragments shorter than    1426 bp
  75% of sequence in fragments shorter than    1583 bp
  80% of sequence in fragments shorter than    1770 bp
  85% of sequence in fragments shorter than    2002 bp
  90% of sequence in fragments shorter than    2311 bp
  95% of sequence in fragments shorter than    2887 bp

# CviQI.PspXI (GTAC+VCTCGAGB) double digest, 5173 thousand fragments (5115078+59193 sites), 575 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     309 bp
  15% of sequence in fragments shorter than     402 bp
  20% of sequence in fragments shorter than     487 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     654 bp
  35% of sequence in fragments shorter than     737 bp
  40% of sequence in fragments shorter than     823 bp
  45% of sequence in fragments shorter than     914 bp
  50% of sequence in fragments shorter than    1012 bp
  55% of sequence in fragments shorter than    1114 bp
  60% of sequence in fragments shorter than    1223 bp
  65% of sequence in fragments shorter than    1345 bp
  70% of sequence in fragments shorter than    1474 bp
  75% of sequence in fragments shorter than    1638 bp
  80% of sequence in fragments shorter than    1838 bp
  85% of sequence in fragments shorter than    2092 bp
  90% of sequence in fragments shorter than    2454 bp
  95% of sequence in fragments shorter than    3103 bp

# AgeI.CviQI (ACCGGT+GTAC) double digest, 5167 thousand fragments (53386+5115078 sites), 576 bp average fragment length
   5% of sequence in fragments shorter than     202 bp
  10% of sequence in fragments shorter than     309 bp
  15% of sequence in fragments shorter than     403 bp
  20% of sequence in fragments shorter than     488 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     656 bp
  35% of sequence in fragments shorter than     739 bp
  40% of sequence in fragments shorter than     825 bp
  45% of sequence in fragments shorter than     917 bp
  50% of sequence in fragments shorter than    1015 bp
  55% of sequence in fragments shorter than    1118 bp
  60% of sequence in fragments shorter than    1228 bp
  65% of sequence in fragments shorter than    1350 bp
  70% of sequence in fragments shorter than    1481 bp
  75% of sequence in fragments shorter than    1646 bp
  80% of sequence in fragments shorter than    1848 bp
  85% of sequence in fragments shorter than    2104 bp
  90% of sequence in fragments shorter than    2470 bp
  95% of sequence in fragments shorter than    3124 bp

# BscGI.MlyI (CCCGT+GAGTC) double digest, 5162 thousand fragments (1231070+3931692 sites), 577 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     294 bp
  15% of sequence in fragments shorter than     368 bp
  20% of sequence in fragments shorter than     460 bp
  25% of sequence in fragments shorter than     549 bp
  30% of sequence in fragments shorter than     640 bp
  35% of sequence in fragments shorter than     728 bp
  40% of sequence in fragments shorter than     825 bp
  45% of sequence in fragments shorter than     926 bp
  50% of sequence in fragments shorter than    1033 bp
  55% of sequence in fragments shorter than    1151 bp
  60% of sequence in fragments shorter than    1282 bp
  65% of sequence in fragments shorter than    1426 bp
  70% of sequence in fragments shorter than    1593 bp
  75% of sequence in fragments shorter than    1788 bp
  80% of sequence in fragments shorter than    2029 bp
  85% of sequence in fragments shorter than    2343 bp
  90% of sequence in fragments shorter than    2786 bp
  95% of sequence in fragments shorter than    3546 bp

# CviQI.PmeI (GTAC+GTTTAAAC) double digest, 5156 thousand fragments (5115078+42094 sites), 577 bp average fragment length
   5% of sequence in fragments shorter than     202 bp
  10% of sequence in fragments shorter than     310 bp
  15% of sequence in fragments shorter than     403 bp
  20% of sequence in fragments shorter than     488 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     656 bp
  35% of sequence in fragments shorter than     740 bp
  40% of sequence in fragments shorter than     826 bp
  45% of sequence in fragments shorter than     918 bp
  50% of sequence in fragments shorter than    1016 bp
  55% of sequence in fragments shorter than    1120 bp
  60% of sequence in fragments shorter than    1230 bp
  65% of sequence in fragments shorter than    1352 bp
  70% of sequence in fragments shorter than    1484 bp
  75% of sequence in fragments shorter than    1649 bp
  80% of sequence in fragments shorter than    1853 bp
  85% of sequence in fragments shorter than    2110 bp
  90% of sequence in fragments shorter than    2478 bp
  95% of sequence in fragments shorter than    3142 bp

# AclI.TfiI (AACGTT+GAWTC) double digest, 5156 thousand fragments (172706+4984362 sites), 577 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     305 bp
  15% of sequence in fragments shorter than     375 bp
  20% of sequence in fragments shorter than     466 bp
  25% of sequence in fragments shorter than     553 bp
  30% of sequence in fragments shorter than     641 bp
  35% of sequence in fragments shorter than     723 bp
  40% of sequence in fragments shorter than     809 bp
  45% of sequence in fragments shorter than     901 bp
  50% of sequence in fragments shorter than    1001 bp
  55% of sequence in fragments shorter than    1105 bp
  60% of sequence in fragments shorter than    1219 bp
  65% of sequence in fragments shorter than    1346 bp
  70% of sequence in fragments shorter than    1489 bp
  75% of sequence in fragments shorter than    1655 bp
  80% of sequence in fragments shorter than    1851 bp
  85% of sequence in fragments shorter than    2098 bp
  90% of sequence in fragments shorter than    2443 bp
  95% of sequence in fragments shorter than    3020 bp

# CviQI.SalI (GTAC+GTCGAC) double digest, 5145 thousand fragments (5115078+31396 sites), 579 bp average fragment length
   5% of sequence in fragments shorter than     202 bp
  10% of sequence in fragments shorter than     310 bp
  15% of sequence in fragments shorter than     404 bp
  20% of sequence in fragments shorter than     490 bp
  25% of sequence in fragments shorter than     572 bp
  30% of sequence in fragments shorter than     658 bp
  35% of sequence in fragments shorter than     741 bp
  40% of sequence in fragments shorter than     828 bp
  45% of sequence in fragments shorter than     919 bp
  50% of sequence in fragments shorter than    1018 bp
  55% of sequence in fragments shorter than    1122 bp
  60% of sequence in fragments shorter than    1231 bp
  65% of sequence in fragments shorter than    1353 bp
  70% of sequence in fragments shorter than    1485 bp
  75% of sequence in fragments shorter than    1651 bp
  80% of sequence in fragments shorter than    1854 bp
  85% of sequence in fragments shorter than    2111 bp
  90% of sequence in fragments shorter than    2478 bp
  95% of sequence in fragments shorter than    3138 bp

# PacI.TfiI (TTAATTAA+GAWTC) double digest, 5143 thousand fragments (159455+4984362 sites), 579 bp average fragment length
   5% of sequence in fragments shorter than     200 bp
  10% of sequence in fragments shorter than     308 bp
  15% of sequence in fragments shorter than     375 bp
  20% of sequence in fragments shorter than     467 bp
  25% of sequence in fragments shorter than     553 bp
  30% of sequence in fragments shorter than     641 bp
  35% of sequence in fragments shorter than     723 bp
  40% of sequence in fragments shorter than     809 bp
  45% of sequence in fragments shorter than     902 bp
  50% of sequence in fragments shorter than    1002 bp
  55% of sequence in fragments shorter than    1106 bp
  60% of sequence in fragments shorter than    1221 bp
  65% of sequence in fragments shorter than    1348 bp
  70% of sequence in fragments shorter than    1491 bp
  75% of sequence in fragments shorter than    1658 bp
  80% of sequence in fragments shorter than    1855 bp
  85% of sequence in fragments shorter than    2102 bp
  90% of sequence in fragments shorter than    2448 bp
  95% of sequence in fragments shorter than    3030 bp

# Hpy99I.TfiI (CGWCG+GAWTC) double digest, 5141 thousand fragments (157917+4984362 sites), 579 bp average fragment length
   5% of sequence in fragments shorter than     200 bp
  10% of sequence in fragments shorter than     308 bp
  15% of sequence in fragments shorter than     376 bp
  20% of sequence in fragments shorter than     467 bp
  25% of sequence in fragments shorter than     554 bp
  30% of sequence in fragments shorter than     642 bp
  35% of sequence in fragments shorter than     723 bp
  40% of sequence in fragments shorter than     809 bp
  45% of sequence in fragments shorter than     902 bp
  50% of sequence in fragments shorter than    1001 bp
  55% of sequence in fragments shorter than    1105 bp
  60% of sequence in fragments shorter than    1219 bp
  65% of sequence in fragments shorter than    1344 bp
  70% of sequence in fragments shorter than    1485 bp
  75% of sequence in fragments shorter than    1649 bp
  80% of sequence in fragments shorter than    1844 bp
  85% of sequence in fragments shorter than    2086 bp
  90% of sequence in fragments shorter than    2424 bp
  95% of sequence in fragments shorter than    2986 bp

# AciI.AvaIII (CCGC+ATGCAT) double digest, 5138 thousand fragments (4206130+933357 sites), 579 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     341 bp
  15% of sequence in fragments shorter than     475 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     736 bp
  30% of sequence in fragments shorter than     866 bp
  35% of sequence in fragments shorter than    1003 bp
  40% of sequence in fragments shorter than    1138 bp
  45% of sequence in fragments shorter than    1287 bp
  50% of sequence in fragments shorter than    1446 bp
  55% of sequence in fragments shorter than    1619 bp
  60% of sequence in fragments shorter than    1814 bp
  65% of sequence in fragments shorter than    2028 bp
  70% of sequence in fragments shorter than    2275 bp
  75% of sequence in fragments shorter than    2566 bp
  80% of sequence in fragments shorter than    2913 bp
  85% of sequence in fragments shorter than    3363 bp
  90% of sequence in fragments shorter than    3999 bp
  95% of sequence in fragments shorter than    5093 bp

# AflIII.BscAI (ACRYGT+GCATC) double digest, 5137 thousand fragments (1485394+3652926 sites), 580 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     307 bp
  15% of sequence in fragments shorter than     399 bp
  20% of sequence in fragments shorter than     489 bp
  25% of sequence in fragments shorter than     577 bp
  30% of sequence in fragments shorter than     661 bp
  35% of sequence in fragments shorter than     748 bp
  40% of sequence in fragments shorter than     838 bp
  45% of sequence in fragments shorter than     934 bp
  50% of sequence in fragments shorter than    1032 bp
  55% of sequence in fragments shorter than    1141 bp
  60% of sequence in fragments shorter than    1257 bp
  65% of sequence in fragments shorter than    1385 bp
  70% of sequence in fragments shorter than    1533 bp
  75% of sequence in fragments shorter than    1697 bp
  80% of sequence in fragments shorter than    1904 bp
  85% of sequence in fragments shorter than    2164 bp
  90% of sequence in fragments shorter than    2544 bp
  95% of sequence in fragments shorter than    3204 bp

# CviQI.MluI (GTAC+ACGCGT) double digest, 5136 thousand fragments (5115078+21415 sites), 580 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     311 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     491 bp
  25% of sequence in fragments shorter than     573 bp
  30% of sequence in fragments shorter than     659 bp
  35% of sequence in fragments shorter than     743 bp
  40% of sequence in fragments shorter than     830 bp
  45% of sequence in fragments shorter than     922 bp
  50% of sequence in fragments shorter than    1020 bp
  55% of sequence in fragments shorter than    1124 bp
  60% of sequence in fragments shorter than    1234 bp
  65% of sequence in fragments shorter than    1356 bp
  70% of sequence in fragments shorter than    1489 bp
  75% of sequence in fragments shorter than    1654 bp
  80% of sequence in fragments shorter than    1858 bp
  85% of sequence in fragments shorter than    2116 bp
  90% of sequence in fragments shorter than    2484 bp
  95% of sequence in fragments shorter than    3144 bp

# EsaBC3I.SfeI (TCGA+CTRYAG) double digest, 5133 thousand fragments (1603346+3531009 sites), 580 bp average fragment length
   5% of sequence in fragments shorter than     199 bp
  10% of sequence in fragments shorter than     307 bp
  15% of sequence in fragments shorter than     398 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     651 bp
  35% of sequence in fragments shorter than     737 bp
  40% of sequence in fragments shorter than     827 bp
  45% of sequence in fragments shorter than     921 bp
  50% of sequence in fragments shorter than    1019 bp
  55% of sequence in fragments shorter than    1123 bp
  60% of sequence in fragments shorter than    1241 bp
  65% of sequence in fragments shorter than    1368 bp
  70% of sequence in fragments shorter than    1510 bp
  75% of sequence in fragments shorter than    1680 bp
  80% of sequence in fragments shorter than    1884 bp
  85% of sequence in fragments shorter than    2134 bp
  90% of sequence in fragments shorter than    2494 bp
  95% of sequence in fragments shorter than    3103 bp

# AciI.StuI (CCGC+AGGCCT) double digest, 5131 thousand fragments (4206130+925729 sites), 580 bp average fragment length
   5% of sequence in fragments shorter than     177 bp
  10% of sequence in fragments shorter than     334 bp
  15% of sequence in fragments shorter than     472 bp
  20% of sequence in fragments shorter than     613 bp
  25% of sequence in fragments shorter than     752 bp
  30% of sequence in fragments shorter than     898 bp
  35% of sequence in fragments shorter than    1058 bp
  40% of sequence in fragments shorter than    1225 bp
  45% of sequence in fragments shorter than    1404 bp
  50% of sequence in fragments shorter than    1606 bp
  55% of sequence in fragments shorter than    1829 bp
  60% of sequence in fragments shorter than    2071 bp
  65% of sequence in fragments shorter than    2355 bp
  70% of sequence in fragments shorter than    2681 bp
  75% of sequence in fragments shorter than    3063 bp
  80% of sequence in fragments shorter than    3533 bp
  85% of sequence in fragments shorter than    4140 bp
  90% of sequence in fragments shorter than    4987 bp
  95% of sequence in fragments shorter than    6435 bp

# CviQI.SgrAI (GTAC+CRCCGGYG) double digest, 5129 thousand fragments (5115078+15270 sites), 580 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     311 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     491 bp
  25% of sequence in fragments shorter than     574 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     831 bp
  45% of sequence in fragments shorter than     923 bp
  50% of sequence in fragments shorter than    1021 bp
  55% of sequence in fragments shorter than    1125 bp
  60% of sequence in fragments shorter than    1235 bp
  65% of sequence in fragments shorter than    1356 bp
  70% of sequence in fragments shorter than    1490 bp
  75% of sequence in fragments shorter than    1656 bp
  80% of sequence in fragments shorter than    1860 bp
  85% of sequence in fragments shorter than    2117 bp
  90% of sequence in fragments shorter than    2485 bp
  95% of sequence in fragments shorter than    3145 bp

# CviQI.NruI (GTAC+TCGCGA) double digest, 5129 thousand fragments (5115078+14872 sites), 580 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     311 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     491 bp
  25% of sequence in fragments shorter than     574 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     831 bp
  45% of sequence in fragments shorter than     922 bp
  50% of sequence in fragments shorter than    1021 bp
  55% of sequence in fragments shorter than    1125 bp
  60% of sequence in fragments shorter than    1235 bp
  65% of sequence in fragments shorter than    1357 bp
  70% of sequence in fragments shorter than    1491 bp
  75% of sequence in fragments shorter than    1656 bp
  80% of sequence in fragments shorter than    1861 bp
  85% of sequence in fragments shorter than    2118 bp
  90% of sequence in fragments shorter than    2488 bp
  95% of sequence in fragments shorter than    3151 bp

# CviQI.PvuI (GTAC+CGATCG) double digest, 5128 thousand fragments (5115078+14186 sites), 581 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     311 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     491 bp
  25% of sequence in fragments shorter than     573 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     831 bp
  45% of sequence in fragments shorter than     923 bp
  50% of sequence in fragments shorter than    1022 bp
  55% of sequence in fragments shorter than    1126 bp
  60% of sequence in fragments shorter than    1236 bp
  65% of sequence in fragments shorter than    1357 bp
  70% of sequence in fragments shorter than    1492 bp
  75% of sequence in fragments shorter than    1657 bp
  80% of sequence in fragments shorter than    1862 bp
  85% of sequence in fragments shorter than    2120 bp
  90% of sequence in fragments shorter than    2489 bp
  95% of sequence in fragments shorter than    3152 bp

# CviQI.FseI (GTAC+GGCCGGCC) double digest, 5128 thousand fragments (5115078+13662 sites), 581 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     312 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     491 bp
  25% of sequence in fragments shorter than     574 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     831 bp
  45% of sequence in fragments shorter than     923 bp
  50% of sequence in fragments shorter than    1021 bp
  55% of sequence in fragments shorter than    1125 bp
  60% of sequence in fragments shorter than    1235 bp
  65% of sequence in fragments shorter than    1356 bp
  70% of sequence in fragments shorter than    1490 bp
  75% of sequence in fragments shorter than    1655 bp
  80% of sequence in fragments shorter than    1859 bp
  85% of sequence in fragments shorter than    2117 bp
  90% of sequence in fragments shorter than    2484 bp
  95% of sequence in fragments shorter than    3144 bp

# AvaII.SspI (GGWCC+AATATT) double digest, 5124 thousand fragments (2747690+2377267 sites), 581 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     307 bp
  15% of sequence in fragments shorter than     395 bp
  20% of sequence in fragments shorter than     484 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     658 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     837 bp
  45% of sequence in fragments shorter than     927 bp
  50% of sequence in fragments shorter than    1023 bp
  55% of sequence in fragments shorter than    1131 bp
  60% of sequence in fragments shorter than    1249 bp
  65% of sequence in fragments shorter than    1381 bp
  70% of sequence in fragments shorter than    1525 bp
  75% of sequence in fragments shorter than    1700 bp
  80% of sequence in fragments shorter than    1905 bp
  85% of sequence in fragments shorter than    2165 bp
  90% of sequence in fragments shorter than    2540 bp
  95% of sequence in fragments shorter than    3190 bp

# CviQI.NotI (GTAC+GCGGCCGC) double digest, 5124 thousand fragments (5115078+9795 sites), 581 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     312 bp
  15% of sequence in fragments shorter than     406 bp
  20% of sequence in fragments shorter than     492 bp
  25% of sequence in fragments shorter than     574 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     832 bp
  45% of sequence in fragments shorter than     924 bp
  50% of sequence in fragments shorter than    1022 bp
  55% of sequence in fragments shorter than    1127 bp
  60% of sequence in fragments shorter than    1236 bp
  65% of sequence in fragments shorter than    1357 bp
  70% of sequence in fragments shorter than    1492 bp
  75% of sequence in fragments shorter than    1657 bp
  80% of sequence in fragments shorter than    1862 bp
  85% of sequence in fragments shorter than    2120 bp
  90% of sequence in fragments shorter than    2489 bp
  95% of sequence in fragments shorter than    3151 bp

# CviQI.RsrII (GTAC+CGGWCCG) double digest, 5124 thousand fragments (5115078+9764 sites), 581 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     312 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     492 bp
  25% of sequence in fragments shorter than     574 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     832 bp
  45% of sequence in fragments shorter than     924 bp
  50% of sequence in fragments shorter than    1022 bp
  55% of sequence in fragments shorter than    1127 bp
  60% of sequence in fragments shorter than    1237 bp
  65% of sequence in fragments shorter than    1358 bp
  70% of sequence in fragments shorter than    1492 bp
  75% of sequence in fragments shorter than    1658 bp
  80% of sequence in fragments shorter than    1862 bp
  85% of sequence in fragments shorter than    2121 bp
  90% of sequence in fragments shorter than    2490 bp
  95% of sequence in fragments shorter than    3153 bp

# AccI.Tsp45I (GTMKAC+GTSAC) double digest, 5122 thousand fragments (1101063+4021761 sites), 581 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     307 bp
  15% of sequence in fragments shorter than     392 bp
  20% of sequence in fragments shorter than     476 bp
  25% of sequence in fragments shorter than     559 bp
  30% of sequence in fragments shorter than     641 bp
  35% of sequence in fragments shorter than     724 bp
  40% of sequence in fragments shorter than     812 bp
  45% of sequence in fragments shorter than     902 bp
  50% of sequence in fragments shorter than     998 bp
  55% of sequence in fragments shorter than    1104 bp
  60% of sequence in fragments shorter than    1218 bp
  65% of sequence in fragments shorter than    1343 bp
  70% of sequence in fragments shorter than    1486 bp
  75% of sequence in fragments shorter than    1655 bp
  80% of sequence in fragments shorter than    1859 bp
  85% of sequence in fragments shorter than    2126 bp
  90% of sequence in fragments shorter than    2510 bp
  95% of sequence in fragments shorter than    3214 bp

# AscI.CviQI (GGCGCGCC+GTAC) double digest, 5119 thousand fragments (4751+5115078 sites), 582 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     312 bp
  15% of sequence in fragments shorter than     406 bp
  20% of sequence in fragments shorter than     492 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     661 bp
  35% of sequence in fragments shorter than     745 bp
  40% of sequence in fragments shorter than     833 bp
  45% of sequence in fragments shorter than     925 bp
  50% of sequence in fragments shorter than    1024 bp
  55% of sequence in fragments shorter than    1128 bp
  60% of sequence in fragments shorter than    1238 bp
  65% of sequence in fragments shorter than    1358 bp
  70% of sequence in fragments shorter than    1494 bp
  75% of sequence in fragments shorter than    1660 bp
  80% of sequence in fragments shorter than    1865 bp
  85% of sequence in fragments shorter than    2124 bp
  90% of sequence in fragments shorter than    2494 bp
  95% of sequence in fragments shorter than    3160 bp

# SnaBI.TfiI (TACGTA+GAWTC) double digest, 5116 thousand fragments (132299+4984362 sites), 582 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     309 bp
  15% of sequence in fragments shorter than     377 bp
  20% of sequence in fragments shorter than     469 bp
  25% of sequence in fragments shorter than     556 bp
  30% of sequence in fragments shorter than     645 bp
  35% of sequence in fragments shorter than     727 bp
  40% of sequence in fragments shorter than     813 bp
  45% of sequence in fragments shorter than     906 bp
  50% of sequence in fragments shorter than    1007 bp
  55% of sequence in fragments shorter than    1112 bp
  60% of sequence in fragments shorter than    1227 bp
  65% of sequence in fragments shorter than    1354 bp
  70% of sequence in fragments shorter than    1499 bp
  75% of sequence in fragments shorter than    1666 bp
  80% of sequence in fragments shorter than    1864 bp
  85% of sequence in fragments shorter than    2111 bp
  90% of sequence in fragments shorter than    2458 bp
  95% of sequence in fragments shorter than    3041 bp

# NaeI.TfiI (GCCGGC+GAWTC) double digest, 5109 thousand fragments (125692+4984362 sites), 583 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     310 bp
  15% of sequence in fragments shorter than     377 bp
  20% of sequence in fragments shorter than     470 bp
  25% of sequence in fragments shorter than     557 bp
  30% of sequence in fragments shorter than     646 bp
  35% of sequence in fragments shorter than     728 bp
  40% of sequence in fragments shorter than     813 bp
  45% of sequence in fragments shorter than     907 bp
  50% of sequence in fragments shorter than    1006 bp
  55% of sequence in fragments shorter than    1110 bp
  60% of sequence in fragments shorter than    1224 bp
  65% of sequence in fragments shorter than    1350 bp
  70% of sequence in fragments shorter than    1492 bp
  75% of sequence in fragments shorter than    1657 bp
  80% of sequence in fragments shorter than    1851 bp
  85% of sequence in fragments shorter than    2094 bp
  90% of sequence in fragments shorter than    2431 bp
  95% of sequence in fragments shorter than    2992 bp

# AciI.NdeI (CCGC+CATATG) double digest, 5108 thousand fragments (4206130+903360 sites), 583 bp average fragment length
   5% of sequence in fragments shorter than     190 bp
  10% of sequence in fragments shorter than     342 bp
  15% of sequence in fragments shorter than     478 bp
  20% of sequence in fragments shorter than     611 bp
  25% of sequence in fragments shorter than     743 bp
  30% of sequence in fragments shorter than     867 bp
  35% of sequence in fragments shorter than    1005 bp
  40% of sequence in fragments shorter than    1144 bp
  45% of sequence in fragments shorter than    1295 bp
  50% of sequence in fragments shorter than    1450 bp
  55% of sequence in fragments shorter than    1626 bp
  60% of sequence in fragments shorter than    1820 bp
  65% of sequence in fragments shorter than    2034 bp
  70% of sequence in fragments shorter than    2277 bp
  75% of sequence in fragments shorter than    2565 bp
  80% of sequence in fragments shorter than    2909 bp
  85% of sequence in fragments shorter than    3350 bp
  90% of sequence in fragments shorter than    3970 bp
  95% of sequence in fragments shorter than    5031 bp

# PpuMI.Tsp45I (RGGWCCY+GTSAC) double digest, 5106 thousand fragments (1085138+4021761 sites), 583 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     306 bp
  15% of sequence in fragments shorter than     391 bp
  20% of sequence in fragments shorter than     480 bp
  25% of sequence in fragments shorter than     566 bp
  30% of sequence in fragments shorter than     655 bp
  35% of sequence in fragments shorter than     745 bp
  40% of sequence in fragments shorter than     841 bp
  45% of sequence in fragments shorter than     939 bp
  50% of sequence in fragments shorter than    1044 bp
  55% of sequence in fragments shorter than    1162 bp
  60% of sequence in fragments shorter than    1290 bp
  65% of sequence in fragments shorter than    1434 bp
  70% of sequence in fragments shorter than    1602 bp
  75% of sequence in fragments shorter than    1796 bp
  80% of sequence in fragments shorter than    2018 bp
  85% of sequence in fragments shorter than    2316 bp
  90% of sequence in fragments shorter than    2731 bp
  95% of sequence in fragments shorter than    3496 bp

# PvuII.Tsp45I (CAGCTG+GTSAC) double digest, 5105 thousand fragments (1084260+4021761 sites), 583 bp average fragment length
   5% of sequence in fragments shorter than     200 bp
  10% of sequence in fragments shorter than     306 bp
  15% of sequence in fragments shorter than     392 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     567 bp
  30% of sequence in fragments shorter than     654 bp
  35% of sequence in fragments shorter than     743 bp
  40% of sequence in fragments shorter than     838 bp
  45% of sequence in fragments shorter than     935 bp
  50% of sequence in fragments shorter than    1040 bp
  55% of sequence in fragments shorter than    1156 bp
  60% of sequence in fragments shorter than    1282 bp
  65% of sequence in fragments shorter than    1422 bp
  70% of sequence in fragments shorter than    1590 bp
  75% of sequence in fragments shorter than    1786 bp
  80% of sequence in fragments shorter than    2029 bp
  85% of sequence in fragments shorter than    2347 bp
  90% of sequence in fragments shorter than    2815 bp
  95% of sequence in fragments shorter than    3702 bp

# TfiI.XhoI (GAWTC+CTCGAG) double digest, 5105 thousand fragments (4984362+121308 sites), 583 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     310 bp
  15% of sequence in fragments shorter than     377 bp
  20% of sequence in fragments shorter than     471 bp
  25% of sequence in fragments shorter than     558 bp
  30% of sequence in fragments shorter than     647 bp
  35% of sequence in fragments shorter than     729 bp
  40% of sequence in fragments shorter than     816 bp
  45% of sequence in fragments shorter than     909 bp
  50% of sequence in fragments shorter than    1010 bp
  55% of sequence in fragments shorter than    1114 bp
  60% of sequence in fragments shorter than    1230 bp
  65% of sequence in fragments shorter than    1356 bp
  70% of sequence in fragments shorter than    1500 bp
  75% of sequence in fragments shorter than    1666 bp
  80% of sequence in fragments shorter than    1863 bp
  85% of sequence in fragments shorter than    2108 bp
  90% of sequence in fragments shorter than    2452 bp
  95% of sequence in fragments shorter than    3028 bp

# SmlI.SspI (CTYRAG+AATATT) double digest, 5104 thousand fragments (2727867+2377267 sites), 583 bp average fragment length
   5% of sequence in fragments shorter than     194 bp
  10% of sequence in fragments shorter than     301 bp
  15% of sequence in fragments shorter than     392 bp
  20% of sequence in fragments shorter than     479 bp
  25% of sequence in fragments shorter than     563 bp
  30% of sequence in fragments shorter than     647 bp
  35% of sequence in fragments shorter than     732 bp
  40% of sequence in fragments shorter than     823 bp
  45% of sequence in fragments shorter than     918 bp
  50% of sequence in fragments shorter than    1018 bp
  55% of sequence in fragments shorter than    1127 bp
  60% of sequence in fragments shorter than    1247 bp
  65% of sequence in fragments shorter than    1379 bp
  70% of sequence in fragments shorter than    1526 bp
  75% of sequence in fragments shorter than    1706 bp
  80% of sequence in fragments shorter than    1914 bp
  85% of sequence in fragments shorter than    2169 bp
  90% of sequence in fragments shorter than    2560 bp
  95% of sequence in fragments shorter than    3286 bp

# BscAI.FauI (GCATC+CCCGC) double digest, 5092 thousand fragments (3652926+1439772 sites), 585 bp average fragment length
   5% of sequence in fragments shorter than     194 bp
  10% of sequence in fragments shorter than     311 bp
  15% of sequence in fragments shorter than     413 bp
  20% of sequence in fragments shorter than     513 bp
  25% of sequence in fragments shorter than     607 bp
  30% of sequence in fragments shorter than     702 bp
  35% of sequence in fragments shorter than     800 bp
  40% of sequence in fragments shorter than     903 bp
  45% of sequence in fragments shorter than    1013 bp
  50% of sequence in fragments shorter than    1130 bp
  55% of sequence in fragments shorter than    1253 bp
  60% of sequence in fragments shorter than    1384 bp
  65% of sequence in fragments shorter than    1536 bp
  70% of sequence in fragments shorter than    1698 bp
  75% of sequence in fragments shorter than    1898 bp
  80% of sequence in fragments shorter than    2134 bp
  85% of sequence in fragments shorter than    2442 bp
  90% of sequence in fragments shorter than    2876 bp
  95% of sequence in fragments shorter than    3653 bp

# BscAI.BspMI (GCATC+ACCTGC) double digest, 5090 thousand fragments (3652926+1438129 sites), 585 bp average fragment length
   5% of sequence in fragments shorter than     199 bp
  10% of sequence in fragments shorter than     308 bp
  15% of sequence in fragments shorter than     401 bp
  20% of sequence in fragments shorter than     492 bp
  25% of sequence in fragments shorter than     582 bp
  30% of sequence in fragments shorter than     666 bp
  35% of sequence in fragments shorter than     755 bp
  40% of sequence in fragments shorter than     841 bp
  45% of sequence in fragments shorter than     938 bp
  50% of sequence in fragments shorter than    1044 bp
  55% of sequence in fragments shorter than    1159 bp
  60% of sequence in fragments shorter than    1281 bp
  65% of sequence in fragments shorter than    1407 bp
  70% of sequence in fragments shorter than    1559 bp
  75% of sequence in fragments shorter than    1736 bp
  80% of sequence in fragments shorter than    1953 bp
  85% of sequence in fragments shorter than    2222 bp
  90% of sequence in fragments shorter than    2609 bp
  95% of sequence in fragments shorter than    3272 bp

# HgiCI.SfeI (GGYRCC+CTRYAG) double digest, 5081 thousand fragments (1550877+3531009 sites), 586 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     314 bp
  15% of sequence in fragments shorter than     408 bp
  20% of sequence in fragments shorter than     496 bp
  25% of sequence in fragments shorter than     584 bp
  30% of sequence in fragments shorter than     670 bp
  35% of sequence in fragments shorter than     761 bp
  40% of sequence in fragments shorter than     857 bp
  45% of sequence in fragments shorter than     953 bp
  50% of sequence in fragments shorter than    1057 bp
  55% of sequence in fragments shorter than    1171 bp
  60% of sequence in fragments shorter than    1287 bp
  65% of sequence in fragments shorter than    1421 bp
  70% of sequence in fragments shorter than    1579 bp
  75% of sequence in fragments shorter than    1762 bp
  80% of sequence in fragments shorter than    1982 bp
  85% of sequence in fragments shorter than    2258 bp
  90% of sequence in fragments shorter than    2661 bp
  95% of sequence in fragments shorter than    3365 bp

# BsmI.SfeI (GAATGC+CTRYAG) double digest, 5079 thousand fragments (1549350+3531009 sites), 586 bp average fragment length
   5% of sequence in fragments shorter than     192 bp
  10% of sequence in fragments shorter than     306 bp
  15% of sequence in fragments shorter than     400 bp
  20% of sequence in fragments shorter than     487 bp
  25% of sequence in fragments shorter than     576 bp
  30% of sequence in fragments shorter than     667 bp
  35% of sequence in fragments shorter than     758 bp
  40% of sequence in fragments shorter than     852 bp
  45% of sequence in fragments shorter than     948 bp
  50% of sequence in fragments shorter than    1047 bp
  55% of sequence in fragments shorter than    1154 bp
  60% of sequence in fragments shorter than    1273 bp
  65% of sequence in fragments shorter than    1405 bp
  70% of sequence in fragments shorter than    1545 bp
  75% of sequence in fragments shorter than    1712 bp
  80% of sequence in fragments shorter than    1911 bp
  85% of sequence in fragments shorter than    2166 bp
  90% of sequence in fragments shorter than    2515 bp
  95% of sequence in fragments shorter than    3099 bp

# ClaI.TfiI (ATCGAT+GAWTC) double digest, 5070 thousand fragments (86477+4984362 sites), 587 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     312 bp
  15% of sequence in fragments shorter than     381 bp
  20% of sequence in fragments shorter than     474 bp
  25% of sequence in fragments shorter than     563 bp
  30% of sequence in fragments shorter than     653 bp
  35% of sequence in fragments shorter than     735 bp
  40% of sequence in fragments shorter than     823 bp
  45% of sequence in fragments shorter than     919 bp
  50% of sequence in fragments shorter than    1020 bp
  55% of sequence in fragments shorter than    1126 bp
  60% of sequence in fragments shorter than    1243 bp
  65% of sequence in fragments shorter than    1371 bp
  70% of sequence in fragments shorter than    1518 bp
  75% of sequence in fragments shorter than    1687 bp
  80% of sequence in fragments shorter than    1886 bp
  85% of sequence in fragments shorter than    2137 bp
  90% of sequence in fragments shorter than    2488 bp
  95% of sequence in fragments shorter than    3078 bp

# AciI.HindIII (CCGC+AAGCTT) double digest, 5066 thousand fragments (4206130+860365 sites), 588 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     344 bp
  15% of sequence in fragments shorter than     484 bp
  20% of sequence in fragments shorter than     616 bp
  25% of sequence in fragments shorter than     751 bp
  30% of sequence in fragments shorter than     884 bp
  35% of sequence in fragments shorter than    1023 bp
  40% of sequence in fragments shorter than    1164 bp
  45% of sequence in fragments shorter than    1321 bp
  50% of sequence in fragments shorter than    1483 bp
  55% of sequence in fragments shorter than    1669 bp
  60% of sequence in fragments shorter than    1874 bp
  65% of sequence in fragments shorter than    2095 bp
  70% of sequence in fragments shorter than    2358 bp
  75% of sequence in fragments shorter than    2661 bp
  80% of sequence in fragments shorter than    3033 bp
  85% of sequence in fragments shorter than    3512 bp
  90% of sequence in fragments shorter than    4185 bp
  95% of sequence in fragments shorter than    5331 bp

# AvaII.HpaII (GGWCC+CCGG) double digest, 5064 thousand fragments (2747690+2317722 sites), 588 bp average fragment length
   5% of sequence in fragments shorter than     183 bp
  10% of sequence in fragments shorter than     310 bp
  15% of sequence in fragments shorter than     424 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     664 bp
  30% of sequence in fragments shorter than     787 bp
  35% of sequence in fragments shorter than     913 bp
  40% of sequence in fragments shorter than    1046 bp
  45% of sequence in fragments shorter than    1195 bp
  50% of sequence in fragments shorter than    1357 bp
  55% of sequence in fragments shorter than    1532 bp
  60% of sequence in fragments shorter than    1726 bp
  65% of sequence in fragments shorter than    1943 bp
  70% of sequence in fragments shorter than    2197 bp
  75% of sequence in fragments shorter than    2497 bp
  80% of sequence in fragments shorter than    2862 bp
  85% of sequence in fragments shorter than    3344 bp
  90% of sequence in fragments shorter than    4041 bp
  95% of sequence in fragments shorter than    5321 bp

# AciI.XbaI (CCGC+TCTAGA) double digest, 5056 thousand fragments (4206130+850998 sites), 589 bp average fragment length
   5% of sequence in fragments shorter than     192 bp
  10% of sequence in fragments shorter than     345 bp
  15% of sequence in fragments shorter than     481 bp
  20% of sequence in fragments shorter than     616 bp
  25% of sequence in fragments shorter than     748 bp
  30% of sequence in fragments shorter than     875 bp
  35% of sequence in fragments shorter than    1018 bp
  40% of sequence in fragments shorter than    1165 bp
  45% of sequence in fragments shorter than    1324 bp
  50% of sequence in fragments shorter than    1488 bp
  55% of sequence in fragments shorter than    1675 bp
  60% of sequence in fragments shorter than    1881 bp
  65% of sequence in fragments shorter than    2110 bp
  70% of sequence in fragments shorter than    2368 bp
  75% of sequence in fragments shorter than    2672 bp
  80% of sequence in fragments shorter than    3040 bp
  85% of sequence in fragments shorter than    3513 bp
  90% of sequence in fragments shorter than    4176 bp
  95% of sequence in fragments shorter than    5298 bp

# AatII.TfiI (GACGTC+GAWTC) double digest, 5054 thousand fragments (71089+4984362 sites), 589 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     313 bp
  15% of sequence in fragments shorter than     381 bp
  20% of sequence in fragments shorter than     475 bp
  25% of sequence in fragments shorter than     564 bp
  30% of sequence in fragments shorter than     653 bp
  35% of sequence in fragments shorter than     736 bp
  40% of sequence in fragments shorter than     823 bp
  45% of sequence in fragments shorter than     918 bp
  50% of sequence in fragments shorter than    1019 bp
  55% of sequence in fragments shorter than    1125 bp
  60% of sequence in fragments shorter than    1242 bp
  65% of sequence in fragments shorter than    1370 bp
  70% of sequence in fragments shorter than    1516 bp
  75% of sequence in fragments shorter than    1684 bp
  80% of sequence in fragments shorter than    1882 bp
  85% of sequence in fragments shorter than    2131 bp
  90% of sequence in fragments shorter than    2481 bp
  95% of sequence in fragments shorter than    3065 bp

# AciI.EcoRI (CCGC+GAATTC) double digest, 5053 thousand fragments (4206130+847401 sites), 589 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     343 bp
  15% of sequence in fragments shorter than     484 bp
  20% of sequence in fragments shorter than     623 bp
  25% of sequence in fragments shorter than     756 bp
  30% of sequence in fragments shorter than     895 bp
  35% of sequence in fragments shorter than    1042 bp
  40% of sequence in fragments shorter than    1192 bp
  45% of sequence in fragments shorter than    1353 bp
  50% of sequence in fragments shorter than    1525 bp
  55% of sequence in fragments shorter than    1717 bp
  60% of sequence in fragments shorter than    1924 bp
  65% of sequence in fragments shorter than    2160 bp
  70% of sequence in fragments shorter than    2424 bp
  75% of sequence in fragments shorter than    2737 bp
  80% of sequence in fragments shorter than    3115 bp
  85% of sequence in fragments shorter than    3604 bp
  90% of sequence in fragments shorter than    4292 bp
  95% of sequence in fragments shorter than    5462 bp

# SacII.TfiI (CCGCGG+GAWTC) double digest, 5050 thousand fragments (66901+4984362 sites), 590 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     313 bp
  15% of sequence in fragments shorter than     382 bp
  20% of sequence in fragments shorter than     476 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     654 bp
  35% of sequence in fragments shorter than     737 bp
  40% of sequence in fragments shorter than     824 bp
  45% of sequence in fragments shorter than     920 bp
  50% of sequence in fragments shorter than    1021 bp
  55% of sequence in fragments shorter than    1126 bp
  60% of sequence in fragments shorter than    1243 bp
  65% of sequence in fragments shorter than    1371 bp
  70% of sequence in fragments shorter than    1516 bp
  75% of sequence in fragments shorter than    1684 bp
  80% of sequence in fragments shorter than    1882 bp
  85% of sequence in fragments shorter than    2130 bp
  90% of sequence in fragments shorter than    2476 bp
  95% of sequence in fragments shorter than    3053 bp

# HpaII.SmlI (CCGG+CTYRAG) double digest, 5045 thousand fragments (2317722+2727867 sites), 590 bp average fragment length
   5% of sequence in fragments shorter than     185 bp
  10% of sequence in fragments shorter than     306 bp
  15% of sequence in fragments shorter than     412 bp
  20% of sequence in fragments shorter than     515 bp
  25% of sequence in fragments shorter than     613 bp
  30% of sequence in fragments shorter than     716 bp
  35% of sequence in fragments shorter than     823 bp
  40% of sequence in fragments shorter than     934 bp
  45% of sequence in fragments shorter than    1051 bp
  50% of sequence in fragments shorter than    1179 bp
  55% of sequence in fragments shorter than    1318 bp
  60% of sequence in fragments shorter than    1469 bp
  65% of sequence in fragments shorter than    1641 bp
  70% of sequence in fragments shorter than    1834 bp
  75% of sequence in fragments shorter than    2064 bp
  80% of sequence in fragments shorter than    2335 bp
  85% of sequence in fragments shorter than    2687 bp
  90% of sequence in fragments shorter than    3210 bp
  95% of sequence in fragments shorter than    4189 bp

# PspXI.TfiI (VCTCGAGB+GAWTC) double digest, 5043 thousand fragments (59193+4984362 sites), 590 bp average fragment length
   5% of sequence in fragments shorter than     204 bp
  10% of sequence in fragments shorter than     313 bp
  15% of sequence in fragments shorter than     382 bp
  20% of sequence in fragments shorter than     476 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     655 bp
  35% of sequence in fragments shorter than     738 bp
  40% of sequence in fragments shorter than     825 bp
  45% of sequence in fragments shorter than     921 bp
  50% of sequence in fragments shorter than    1022 bp
  55% of sequence in fragments shorter than    1128 bp
  60% of sequence in fragments shorter than    1244 bp
  65% of sequence in fragments shorter than    1373 bp
  70% of sequence in fragments shorter than    1519 bp
  75% of sequence in fragments shorter than    1688 bp
  80% of sequence in fragments shorter than    1886 bp
  85% of sequence in fragments shorter than    2135 bp
  90% of sequence in fragments shorter than    2483 bp
  95% of sequence in fragments shorter than    3068 bp

# AgeI.TfiI (ACCGGT+GAWTC) double digest, 5037 thousand fragments (53386+4984362 sites), 591 bp average fragment length
   5% of sequence in fragments shorter than     204 bp
  10% of sequence in fragments shorter than     314 bp
  15% of sequence in fragments shorter than     382 bp
  20% of sequence in fragments shorter than     476 bp
  25% of sequence in fragments shorter than     565 bp
  30% of sequence in fragments shorter than     655 bp
  35% of sequence in fragments shorter than     738 bp
  40% of sequence in fragments shorter than     827 bp
  45% of sequence in fragments shorter than     923 bp
  50% of sequence in fragments shorter than    1024 bp
  55% of sequence in fragments shorter than    1131 bp
  60% of sequence in fragments shorter than    1248 bp
  65% of sequence in fragments shorter than    1377 bp
  70% of sequence in fragments shorter than    1523 bp
  75% of sequence in fragments shorter than    1693 bp
  80% of sequence in fragments shorter than    1892 bp
  85% of sequence in fragments shorter than    2143 bp
  90% of sequence in fragments shorter than    2495 bp
  95% of sequence in fragments shorter than    3083 bp

# AccI.MlyI (GTMKAC+GAGTC) double digest, 5032 thousand fragments (1101063+3931692 sites), 592 bp average fragment length
   5% of sequence in fragments shorter than     209 bp
  10% of sequence in fragments shorter than     315 bp
  15% of sequence in fragments shorter than     402 bp
  20% of sequence in fragments shorter than     487 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     653 bp
  35% of sequence in fragments shorter than     738 bp
  40% of sequence in fragments shorter than     825 bp
  45% of sequence in fragments shorter than     915 bp
  50% of sequence in fragments shorter than    1012 bp
  55% of sequence in fragments shorter than    1117 bp
  60% of sequence in fragments shorter than    1231 bp
  65% of sequence in fragments shorter than    1358 bp
  70% of sequence in fragments shorter than    1499 bp
  75% of sequence in fragments shorter than    1669 bp
  80% of sequence in fragments shorter than    1868 bp
  85% of sequence in fragments shorter than    2130 bp
  90% of sequence in fragments shorter than    2513 bp
  95% of sequence in fragments shorter than    3161 bp

# AvaI.Tsp45I (CYCGRG+GTSAC) double digest, 5026 thousand fragments (1005714+4021761 sites), 592 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     311 bp
  15% of sequence in fragments shorter than     400 bp
  20% of sequence in fragments shorter than     490 bp
  25% of sequence in fragments shorter than     578 bp
  30% of sequence in fragments shorter than     669 bp
  35% of sequence in fragments shorter than     761 bp
  40% of sequence in fragments shorter than     859 bp
  45% of sequence in fragments shorter than     960 bp
  50% of sequence in fragments shorter than    1070 bp
  55% of sequence in fragments shorter than    1191 bp
  60% of sequence in fragments shorter than    1324 bp
  65% of sequence in fragments shorter than    1474 bp
  70% of sequence in fragments shorter than    1651 bp
  75% of sequence in fragments shorter than    1857 bp
  80% of sequence in fragments shorter than    2112 bp
  85% of sequence in fragments shorter than    2451 bp
  90% of sequence in fragments shorter than    2958 bp
  95% of sequence in fragments shorter than    3920 bp

# PmeI.TfiI (GTTTAAAC+GAWTC) double digest, 5025 thousand fragments (42094+4984362 sites), 592 bp average fragment length
   5% of sequence in fragments shorter than     204 bp
  10% of sequence in fragments shorter than     314 bp
  15% of sequence in fragments shorter than     384 bp
  20% of sequence in fragments shorter than     478 bp
  25% of sequence in fragments shorter than     567 bp
  30% of sequence in fragments shorter than     657 bp
  35% of sequence in fragments shorter than     740 bp
  40% of sequence in fragments shorter than     828 bp
  45% of sequence in fragments shorter than     924 bp
  50% of sequence in fragments shorter than    1026 bp
  55% of sequence in fragments shorter than    1133 bp
  60% of sequence in fragments shorter than    1251 bp
  65% of sequence in fragments shorter than    1380 bp
  70% of sequence in fragments shorter than    1527 bp
  75% of sequence in fragments shorter than    1698 bp
  80% of sequence in fragments shorter than    1899 bp
  85% of sequence in fragments shorter than    2151 bp
  90% of sequence in fragments shorter than    2505 bp
  95% of sequence in fragments shorter than    3099 bp

# MlyI.PpuMI (GAGTC+RGGWCCY) double digest, 5016 thousand fragments (3931692+1085138 sites), 594 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     308 bp
  15% of sequence in fragments shorter than     397 bp
  20% of sequence in fragments shorter than     486 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     665 bp
  35% of sequence in fragments shorter than     757 bp
  40% of sequence in fragments shorter than     853 bp
  45% of sequence in fragments shorter than     951 bp
  50% of sequence in fragments shorter than    1059 bp
  55% of sequence in fragments shorter than    1178 bp
  60% of sequence in fragments shorter than    1307 bp
  65% of sequence in fragments shorter than    1453 bp
  70% of sequence in fragments shorter than    1622 bp
  75% of sequence in fragments shorter than    1822 bp
  80% of sequence in fragments shorter than    2058 bp
  85% of sequence in fragments shorter than    2375 bp
  90% of sequence in fragments shorter than    2848 bp
  95% of sequence in fragments shorter than    3748 bp

# AflIII.SfeI (ACRYGT+CTRYAG) double digest, 5015 thousand fragments (1485394+3531009 sites), 594 bp average fragment length
   5% of sequence in fragments shorter than     210 bp
  10% of sequence in fragments shorter than     317 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     488 bp
  25% of sequence in fragments shorter than     571 bp
  30% of sequence in fragments shorter than     657 bp
  35% of sequence in fragments shorter than     738 bp
  40% of sequence in fragments shorter than     827 bp
  45% of sequence in fragments shorter than     916 bp
  50% of sequence in fragments shorter than    1011 bp
  55% of sequence in fragments shorter than    1110 bp
  60% of sequence in fragments shorter than    1224 bp
  65% of sequence in fragments shorter than    1349 bp
  70% of sequence in fragments shorter than    1484 bp
  75% of sequence in fragments shorter than    1650 bp
  80% of sequence in fragments shorter than    1845 bp
  85% of sequence in fragments shorter than    2093 bp
  90% of sequence in fragments shorter than    2413 bp
  95% of sequence in fragments shorter than    2997 bp

# MlyI.PvuII (GAGTC+CAGCTG) double digest, 5015 thousand fragments (3931692+1084260 sites), 594 bp average fragment length
   5% of sequence in fragments shorter than     202 bp
  10% of sequence in fragments shorter than     308 bp
  15% of sequence in fragments shorter than     399 bp
  20% of sequence in fragments shorter than     487 bp
  25% of sequence in fragments shorter than     575 bp
  30% of sequence in fragments shorter than     663 bp
  35% of sequence in fragments shorter than     754 bp
  40% of sequence in fragments shorter than     849 bp
  45% of sequence in fragments shorter than     948 bp
  50% of sequence in fragments shorter than    1054 bp
  55% of sequence in fragments shorter than    1172 bp
  60% of sequence in fragments shorter than    1304 bp
  65% of sequence in fragments shorter than    1444 bp
  70% of sequence in fragments shorter than    1613 bp
  75% of sequence in fragments shorter than    1814 bp
  80% of sequence in fragments shorter than    2068 bp
  85% of sequence in fragments shorter than    2404 bp
  90% of sequence in fragments shorter than    2888 bp
  95% of sequence in fragments shorter than    3808 bp

# SalI.TfiI (GTCGAC+GAWTC) double digest, 5015 thousand fragments (31396+4984362 sites), 594 bp average fragment length
   5% of sequence in fragments shorter than     205 bp
  10% of sequence in fragments shorter than     315 bp
  15% of sequence in fragments shorter than     384 bp
  20% of sequence in fragments shorter than     479 bp
  25% of sequence in fragments shorter than     568 bp
  30% of sequence in fragments shorter than     659 bp
  35% of sequence in fragments shorter than     742 bp
  40% of sequence in fragments shorter than     830 bp
  45% of sequence in fragments shorter than     927 bp
  50% of sequence in fragments shorter than    1028 bp
  55% of sequence in fragments shorter than    1135 bp
  60% of sequence in fragments shorter than    1253 bp
  65% of sequence in fragments shorter than    1383 bp
  70% of sequence in fragments shorter than    1530 bp
  75% of sequence in fragments shorter than    1701 bp
  80% of sequence in fragments shorter than    1902 bp
  85% of sequence in fragments shorter than    2154 bp
  90% of sequence in fragments shorter than    2508 bp
  95% of sequence in fragments shorter than    3100 bp

# MluI.TfiI (ACGCGT+GAWTC) double digest, 5005 thousand fragments (21415+4984362 sites), 595 bp average fragment length
   5% of sequence in fragments shorter than     205 bp
  10% of sequence in fragments shorter than     315 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     480 bp
  25% of sequence in fragments shorter than     570 bp
  30% of sequence in fragments shorter than     660 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     832 bp
  45% of sequence in fragments shorter than     929 bp
  50% of sequence in fragments shorter than    1031 bp
  55% of sequence in fragments shorter than    1138 bp
  60% of sequence in fragments shorter than    1256 bp
  65% of sequence in fragments shorter than    1385 bp
  70% of sequence in fragments shorter than    1533 bp
  75% of sequence in fragments shorter than    1704 bp
  80% of sequence in fragments shorter than    1905 bp
  85% of sequence in fragments shorter than    2158 bp
  90% of sequence in fragments shorter than    2511 bp
  95% of sequence in fragments shorter than    3104 bp

# BspHI.Tsp45I (TCATGA+GTSAC) double digest, 4999 thousand fragments (978290+4021761 sites), 596 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     315 bp
  15% of sequence in fragments shorter than     402 bp
  20% of sequence in fragments shorter than     490 bp
  25% of sequence in fragments shorter than     573 bp
  30% of sequence in fragments shorter than     659 bp
  35% of sequence in fragments shorter than     744 bp
  40% of sequence in fragments shorter than     835 bp
  45% of sequence in fragments shorter than     928 bp
  50% of sequence in fragments shorter than    1029 bp
  55% of sequence in fragments shorter than    1135 bp
  60% of sequence in fragments shorter than    1254 bp
  65% of sequence in fragments shorter than    1384 bp
  70% of sequence in fragments shorter than    1536 bp
  75% of sequence in fragments shorter than    1709 bp
  80% of sequence in fragments shorter than    1909 bp
  85% of sequence in fragments shorter than    2172 bp
  90% of sequence in fragments shorter than    2527 bp
  95% of sequence in fragments shorter than    3178 bp

# SgrAI.TfiI (CRCCGGYG+GAWTC) double digest, 4999 thousand fragments (15270+4984362 sites), 596 bp average fragment length
   5% of sequence in fragments shorter than     205 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     571 bp
  30% of sequence in fragments shorter than     661 bp
  35% of sequence in fragments shorter than     745 bp
  40% of sequence in fragments shorter than     833 bp
  45% of sequence in fragments shorter than     929 bp
  50% of sequence in fragments shorter than    1031 bp
  55% of sequence in fragments shorter than    1138 bp
  60% of sequence in fragments shorter than    1257 bp
  65% of sequence in fragments shorter than    1386 bp
  70% of sequence in fragments shorter than    1534 bp
  75% of sequence in fragments shorter than    1705 bp
  80% of sequence in fragments shorter than    1905 bp
  85% of sequence in fragments shorter than    2158 bp
  90% of sequence in fragments shorter than    2511 bp
  95% of sequence in fragments shorter than    3103 bp

# NruI.TfiI (TCGCGA+GAWTC) double digest, 4998 thousand fragments (14872+4984362 sites), 596 bp average fragment length
   5% of sequence in fragments shorter than     205 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     571 bp
  30% of sequence in fragments shorter than     661 bp
  35% of sequence in fragments shorter than     745 bp
  40% of sequence in fragments shorter than     834 bp
  45% of sequence in fragments shorter than     930 bp
  50% of sequence in fragments shorter than    1032 bp
  55% of sequence in fragments shorter than    1140 bp
  60% of sequence in fragments shorter than    1258 bp
  65% of sequence in fragments shorter than    1388 bp
  70% of sequence in fragments shorter than    1536 bp
  75% of sequence in fragments shorter than    1708 bp
  80% of sequence in fragments shorter than    1909 bp
  85% of sequence in fragments shorter than    2163 bp
  90% of sequence in fragments shorter than    2517 bp
  95% of sequence in fragments shorter than    3113 bp

# PvuI.TfiI (CGATCG+GAWTC) double digest, 4998 thousand fragments (14186+4984362 sites), 596 bp average fragment length
   5% of sequence in fragments shorter than     205 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     571 bp
  30% of sequence in fragments shorter than     662 bp
  35% of sequence in fragments shorter than     745 bp
  40% of sequence in fragments shorter than     834 bp
  45% of sequence in fragments shorter than     930 bp
  50% of sequence in fragments shorter than    1032 bp
  55% of sequence in fragments shorter than    1140 bp
  60% of sequence in fragments shorter than    1259 bp
  65% of sequence in fragments shorter than    1389 bp
  70% of sequence in fragments shorter than    1537 bp
  75% of sequence in fragments shorter than    1708 bp
  80% of sequence in fragments shorter than    1910 bp
  85% of sequence in fragments shorter than    2164 bp
  90% of sequence in fragments shorter than    2518 bp
  95% of sequence in fragments shorter than    3114 bp

# FseI.TfiI (GGCCGGCC+GAWTC) double digest, 4997 thousand fragments (13662+4984362 sites), 596 bp average fragment length
   5% of sequence in fragments shorter than     205 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     571 bp
  30% of sequence in fragments shorter than     661 bp
  35% of sequence in fragments shorter than     745 bp
  40% of sequence in fragments shorter than     833 bp
  45% of sequence in fragments shorter than     930 bp
  50% of sequence in fragments shorter than    1032 bp
  55% of sequence in fragments shorter than    1139 bp
  60% of sequence in fragments shorter than    1257 bp
  65% of sequence in fragments shorter than    1387 bp
  70% of sequence in fragments shorter than    1534 bp
  75% of sequence in fragments shorter than    1705 bp
  80% of sequence in fragments shorter than    1906 bp
  85% of sequence in fragments shorter than    2159 bp
  90% of sequence in fragments shorter than    2512 bp
  95% of sequence in fragments shorter than    3103 bp

# NotI.TfiI (GCGGCCGC+GAWTC) double digest, 4993 thousand fragments (9795+4984362 sites), 596 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     571 bp
  30% of sequence in fragments shorter than     662 bp
  35% of sequence in fragments shorter than     746 bp
  40% of sequence in fragments shorter than     834 bp
  45% of sequence in fragments shorter than     931 bp
  50% of sequence in fragments shorter than    1032 bp
  55% of sequence in fragments shorter than    1140 bp
  60% of sequence in fragments shorter than    1258 bp
  65% of sequence in fragments shorter than    1388 bp
  70% of sequence in fragments shorter than    1536 bp
  75% of sequence in fragments shorter than    1707 bp
  80% of sequence in fragments shorter than    1908 bp
  85% of sequence in fragments shorter than    2162 bp
  90% of sequence in fragments shorter than    2515 bp
  95% of sequence in fragments shorter than    3108 bp

# RsrII.TfiI (CGGWCCG+GAWTC) double digest, 4993 thousand fragments (9764+4984362 sites), 596 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     386 bp
  20% of sequence in fragments shorter than     481 bp
  25% of sequence in fragments shorter than     571 bp
  30% of sequence in fragments shorter than     662 bp
  35% of sequence in fragments shorter than     746 bp
  40% of sequence in fragments shorter than     834 bp
  45% of sequence in fragments shorter than     931 bp
  50% of sequence in fragments shorter than    1033 bp
  55% of sequence in fragments shorter than    1140 bp
  60% of sequence in fragments shorter than    1259 bp
  65% of sequence in fragments shorter than    1389 bp
  70% of sequence in fragments shorter than    1536 bp
  75% of sequence in fragments shorter than    1708 bp
  80% of sequence in fragments shorter than    1909 bp
  85% of sequence in fragments shorter than    2163 bp
  90% of sequence in fragments shorter than    2517 bp
  95% of sequence in fragments shorter than    3111 bp

# AscI.TfiI (GGCGCGCC+GAWTC) double digest, 4988 thousand fragments (4751+4984362 sites), 597 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     387 bp
  20% of sequence in fragments shorter than     482 bp
  25% of sequence in fragments shorter than     572 bp
  30% of sequence in fragments shorter than     663 bp
  35% of sequence in fragments shorter than     747 bp
  40% of sequence in fragments shorter than     835 bp
  45% of sequence in fragments shorter than     932 bp
  50% of sequence in fragments shorter than    1033 bp
  55% of sequence in fragments shorter than    1142 bp
  60% of sequence in fragments shorter than    1260 bp
  65% of sequence in fragments shorter than    1391 bp
  70% of sequence in fragments shorter than    1539 bp
  75% of sequence in fragments shorter than    1711 bp
  80% of sequence in fragments shorter than    1912 bp
  85% of sequence in fragments shorter than    2166 bp
  90% of sequence in fragments shorter than    2521 bp
  95% of sequence in fragments shorter than    3118 bp

# AciI.BglII (CCGC+AGATCT) double digest, 4980 thousand fragments (4206130+774732 sites), 598 bp average fragment length
   5% of sequence in fragments shorter than     194 bp
  10% of sequence in fragments shorter than     349 bp
  15% of sequence in fragments shorter than     496 bp
  20% of sequence in fragments shorter than     635 bp
  25% of sequence in fragments shorter than     774 bp
  30% of sequence in fragments shorter than     914 bp
  35% of sequence in fragments shorter than    1057 bp
  40% of sequence in fragments shorter than    1213 bp
  45% of sequence in fragments shorter than    1378 bp
  50% of sequence in fragments shorter than    1545 bp
  55% of sequence in fragments shorter than    1737 bp
  60% of sequence in fragments shorter than    1950 bp
  65% of sequence in fragments shorter than    2190 bp
  70% of sequence in fragments shorter than    2459 bp
  75% of sequence in fragments shorter than    2775 bp
  80% of sequence in fragments shorter than    3161 bp
  85% of sequence in fragments shorter than    3663 bp
  90% of sequence in fragments shorter than    4368 bp
  95% of sequence in fragments shorter than    5601 bp

# BscAI.PstI (GCATC+CTGCAG) double digest, 4973 thousand fragments (3652926+1321469 sites), 599 bp average fragment length
   5% of sequence in fragments shorter than     202 bp
  10% of sequence in fragments shorter than     313 bp
  15% of sequence in fragments shorter than     411 bp
  20% of sequence in fragments shorter than     506 bp
  25% of sequence in fragments shorter than     597 bp
  30% of sequence in fragments shorter than     686 bp
  35% of sequence in fragments shorter than     780 bp
  40% of sequence in fragments shorter than     877 bp
  45% of sequence in fragments shorter than     982 bp
  50% of sequence in fragments shorter than    1093 bp
  55% of sequence in fragments shorter than    1211 bp
  60% of sequence in fragments shorter than    1340 bp
  65% of sequence in fragments shorter than    1480 bp
  70% of sequence in fragments shorter than    1636 bp
  75% of sequence in fragments shorter than    1824 bp
  80% of sequence in fragments shorter than    2053 bp
  85% of sequence in fragments shorter than    2340 bp
  90% of sequence in fragments shorter than    2753 bp
  95% of sequence in fragments shorter than    3470 bp

# FauI.SfeI (CCCGC+CTRYAG) double digest, 4970 thousand fragments (1439772+3531009 sites), 599 bp average fragment length
   5% of sequence in fragments shorter than     201 bp
  10% of sequence in fragments shorter than     322 bp
  15% of sequence in fragments shorter than     427 bp
  20% of sequence in fragments shorter than     525 bp
  25% of sequence in fragments shorter than     626 bp
  30% of sequence in fragments shorter than     722 bp
  35% of sequence in fragments shorter than     826 bp
  40% of sequence in fragments shorter than     932 bp
  45% of sequence in fragments shorter than    1044 bp
  50% of sequence in fragments shorter than    1162 bp
  55% of sequence in fragments shorter than    1290 bp
  60% of sequence in fragments shorter than    1426 bp
  65% of sequence in fragments shorter than    1584 bp
  70% of sequence in fragments shorter than    1761 bp
  75% of sequence in fragments shorter than    1967 bp
  80% of sequence in fragments shorter than    2196 bp
  85% of sequence in fragments shorter than    2513 bp
  90% of sequence in fragments shorter than    2957 bp
  95% of sequence in fragments shorter than    3742 bp

# BspMI.SfeI (ACCTGC+CTRYAG) double digest, 4968 thousand fragments (1438129+3531009 sites), 599 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     325 bp
  15% of sequence in fragments shorter than     417 bp
  20% of sequence in fragments shorter than     505 bp
  25% of sequence in fragments shorter than     597 bp
  30% of sequence in fragments shorter than     684 bp
  35% of sequence in fragments shorter than     774 bp
  40% of sequence in fragments shorter than     869 bp
  45% of sequence in fragments shorter than     963 bp
  50% of sequence in fragments shorter than    1068 bp
  55% of sequence in fragments shorter than    1180 bp
  60% of sequence in fragments shorter than    1305 bp
  65% of sequence in fragments shorter than    1434 bp
  70% of sequence in fragments shorter than    1589 bp
  75% of sequence in fragments shorter than    1770 bp
  80% of sequence in fragments shorter than    1963 bp
  85% of sequence in fragments shorter than    2232 bp
  90% of sequence in fragments shorter than    2614 bp
  95% of sequence in fragments shorter than    3270 bp

# AciI.NcoI (CCGC+CCATGG) double digest, 4965 thousand fragments (4206130+759594 sites), 600 bp average fragment length
   5% of sequence in fragments shorter than     187 bp
  10% of sequence in fragments shorter than     346 bp
  15% of sequence in fragments shorter than     498 bp
  20% of sequence in fragments shorter than     646 bp
  25% of sequence in fragments shorter than     797 bp
  30% of sequence in fragments shorter than     946 bp
  35% of sequence in fragments shorter than    1104 bp
  40% of sequence in fragments shorter than    1275 bp
  45% of sequence in fragments shorter than    1451 bp
  50% of sequence in fragments shorter than    1649 bp
  55% of sequence in fragments shorter than    1870 bp
  60% of sequence in fragments shorter than    2111 bp
  65% of sequence in fragments shorter than    2388 bp
  70% of sequence in fragments shorter than    2701 bp
  75% of sequence in fragments shorter than    3070 bp
  80% of sequence in fragments shorter than    3523 bp
  85% of sequence in fragments shorter than    4111 bp
  90% of sequence in fragments shorter than    4936 bp
  95% of sequence in fragments shorter than    6376 bp

# AvaIII.Tsp45I (ATGCAT+GTSAC) double digest, 4954 thousand fragments (933357+4021761 sites), 601 bp average fragment length
   5% of sequence in fragments shorter than     210 bp
  10% of sequence in fragments shorter than     318 bp
  15% of sequence in fragments shorter than     406 bp
  20% of sequence in fragments shorter than     494 bp
  25% of sequence in fragments shorter than     578 bp
  30% of sequence in fragments shorter than     665 bp
  35% of sequence in fragments shorter than     750 bp
  40% of sequence in fragments shorter than     842 bp
  45% of sequence in fragments shorter than     935 bp
  50% of sequence in fragments shorter than    1036 bp
  55% of sequence in fragments shorter than    1144 bp
  60% of sequence in fragments shorter than    1265 bp
  65% of sequence in fragments shorter than    1398 bp
  70% of sequence in fragments shorter than    1553 bp
  75% of sequence in fragments shorter than    1730 bp
  80% of sequence in fragments shorter than    1953 bp
  85% of sequence in fragments shorter than    2243 bp
  90% of sequence in fragments shorter than    2668 bp
  95% of sequence in fragments shorter than    3480 bp

# StuI.Tsp45I (AGGCCT+GTSAC) double digest, 4946 thousand fragments (925729+4021761 sites), 602 bp average fragment length
   5% of sequence in fragments shorter than     207 bp
  10% of sequence in fragments shorter than     315 bp
  15% of sequence in fragments shorter than     402 bp
  20% of sequence in fragments shorter than     491 bp
  25% of sequence in fragments shorter than     579 bp
  30% of sequence in fragments shorter than     669 bp
  35% of sequence in fragments shorter than     758 bp
  40% of sequence in fragments shorter than     856 bp
  45% of sequence in fragments shorter than     956 bp
  50% of sequence in fragments shorter than    1063 bp
  55% of sequence in fragments shorter than    1182 bp
  60% of sequence in fragments shorter than    1312 bp
  65% of sequence in fragments shorter than    1452 bp
  70% of sequence in fragments shorter than    1618 bp
  75% of sequence in fragments shorter than    1812 bp
  80% of sequence in fragments shorter than    2037 bp
  85% of sequence in fragments shorter than    2337 bp
  90% of sequence in fragments shorter than    2754 bp
  95% of sequence in fragments shorter than    3473 bp

# AciI.BclI (CCGC+TGATCA) double digest, 4944 thousand fragments (4206130+738785 sites), 602 bp average fragment length
   5% of sequence in fragments shorter than     196 bp
  10% of sequence in fragments shorter than     354 bp
  15% of sequence in fragments shorter than     501 bp
  20% of sequence in fragments shorter than     640 bp
  25% of sequence in fragments shorter than     781 bp
  30% of sequence in fragments shorter than     915 bp
  35% of sequence in fragments shorter than    1059 bp
  40% of sequence in fragments shorter than    1216 bp
  45% of sequence in fragments shorter than    1380 bp
  50% of sequence in fragments shorter than    1556 bp
  55% of sequence in fragments shorter than    1751 bp
  60% of sequence in fragments shorter than    1965 bp
  65% of sequence in fragments shorter than    2206 bp
  70% of sequence in fragments shorter than    2479 bp
  75% of sequence in fragments shorter than    2802 bp
  80% of sequence in fragments shorter than    3199 bp
  85% of sequence in fragments shorter than    3707 bp
  90% of sequence in fragments shorter than    4425 bp
  95% of sequence in fragments shorter than    5651 bp

# AciI.FnuDII (CCGC+CGCG) double digest, 4939 thousand fragments (4206130+733940 sites), 603 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     404 bp
  15% of sequence in fragments shorter than     595 bp
  20% of sequence in fragments shorter than     785 bp
  25% of sequence in fragments shorter than     974 bp
  30% of sequence in fragments shorter than    1172 bp
  35% of sequence in fragments shorter than    1381 bp
  40% of sequence in fragments shorter than    1608 bp
  45% of sequence in fragments shorter than    1861 bp
  50% of sequence in fragments shorter than    2135 bp
  55% of sequence in fragments shorter than    2440 bp
  60% of sequence in fragments shorter than    2779 bp
  65% of sequence in fragments shorter than    3165 bp
  70% of sequence in fragments shorter than    3619 bp
  75% of sequence in fragments shorter than    4151 bp
  80% of sequence in fragments shorter than    4807 bp
  85% of sequence in fragments shorter than    5655 bp
  90% of sequence in fragments shorter than    6853 bp
  95% of sequence in fragments shorter than    8934 bp

# AvaI.MlyI (CYCGRG+GAGTC) double digest, 4936 thousand fragments (1005714+3931692 sites), 603 bp average fragment length
   5% of sequence in fragments shorter than     202 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     414 bp
  20% of sequence in fragments shorter than     510 bp
  25% of sequence in fragments shorter than     603 bp
  30% of sequence in fragments shorter than     699 bp
  35% of sequence in fragments shorter than     798 bp
  40% of sequence in fragments shorter than     899 bp
  45% of sequence in fragments shorter than    1008 bp
  50% of sequence in fragments shorter than    1124 bp
  55% of sequence in fragments shorter than    1255 bp
  60% of sequence in fragments shorter than    1397 bp
  65% of sequence in fragments shorter than    1559 bp
  70% of sequence in fragments shorter than    1744 bp
  75% of sequence in fragments shorter than    1967 bp
  80% of sequence in fragments shorter than    2246 bp
  85% of sequence in fragments shorter than    2621 bp
  90% of sequence in fragments shorter than    3161 bp
  95% of sequence in fragments shorter than    4188 bp

# NdeI.Tsp45I (CATATG+GTSAC) double digest, 4924 thousand fragments (903360+4021761 sites), 605 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     321 bp
  15% of sequence in fragments shorter than     410 bp
  20% of sequence in fragments shorter than     498 bp
  25% of sequence in fragments shorter than     583 bp
  30% of sequence in fragments shorter than     670 bp
  35% of sequence in fragments shorter than     755 bp
  40% of sequence in fragments shorter than     843 bp
  45% of sequence in fragments shorter than     935 bp
  50% of sequence in fragments shorter than    1034 bp
  55% of sequence in fragments shorter than    1141 bp
  60% of sequence in fragments shorter than    1250 bp
  65% of sequence in fragments shorter than    1377 bp
  70% of sequence in fragments shorter than    1523 bp
  75% of sequence in fragments shorter than    1691 bp
  80% of sequence in fragments shorter than    1893 bp
  85% of sequence in fragments shorter than    2149 bp
  90% of sequence in fragments shorter than    2495 bp
  95% of sequence in fragments shorter than    3107 bp

# AciI.BsaAI (CCGC+YACGTR) double digest, 4919 thousand fragments (4206130+713801 sites), 605 bp average fragment length
   5% of sequence in fragments shorter than     191 bp
  10% of sequence in fragments shorter than     353 bp
  15% of sequence in fragments shorter than     504 bp
  20% of sequence in fragments shorter than     653 bp
  25% of sequence in fragments shorter than     804 bp
  30% of sequence in fragments shorter than     955 bp
  35% of sequence in fragments shorter than    1117 bp
  40% of sequence in fragments shorter than    1290 bp
  45% of sequence in fragments shorter than    1468 bp
  50% of sequence in fragments shorter than    1672 bp
  55% of sequence in fragments shorter than    1895 bp
  60% of sequence in fragments shorter than    2141 bp
  65% of sequence in fragments shorter than    2419 bp
  70% of sequence in fragments shorter than    2736 bp
  75% of sequence in fragments shorter than    3109 bp
  80% of sequence in fragments shorter than    3571 bp
  85% of sequence in fragments shorter than    4166 bp
  90% of sequence in fragments shorter than    4997 bp
  95% of sequence in fragments shorter than    6429 bp

# AciI.BceAI (CCGC+ACGGC) double digest, 4910 thousand fragments (4206130+705178 sites), 606 bp average fragment length
   5% of sequence in fragments shorter than     188 bp
  10% of sequence in fragments shorter than     355 bp
  15% of sequence in fragments shorter than     519 bp
  20% of sequence in fragments shorter than     683 bp
  25% of sequence in fragments shorter than     846 bp
  30% of sequence in fragments shorter than    1017 bp
  35% of sequence in fragments shorter than    1195 bp
  40% of sequence in fragments shorter than    1385 bp
  45% of sequence in fragments shorter than    1593 bp
  50% of sequence in fragments shorter than    1825 bp
  55% of sequence in fragments shorter than    2077 bp
  60% of sequence in fragments shorter than    2364 bp
  65% of sequence in fragments shorter than    2684 bp
  70% of sequence in fragments shorter than    3056 bp
  75% of sequence in fragments shorter than    3500 bp
  80% of sequence in fragments shorter than    4051 bp
  85% of sequence in fragments shorter than    4759 bp
  90% of sequence in fragments shorter than    5759 bp
  95% of sequence in fragments shorter than    7490 bp

# BspHI.MlyI (TCATGA+GAGTC) double digest, 4909 thousand fragments (978290+3931692 sites), 606 bp average fragment length
   5% of sequence in fragments shorter than     211 bp
  10% of sequence in fragments shorter than     317 bp
  15% of sequence in fragments shorter than     410 bp
  20% of sequence in fragments shorter than     498 bp
  25% of sequence in fragments shorter than     585 bp
  30% of sequence in fragments shorter than     673 bp
  35% of sequence in fragments shorter than     760 bp
  40% of sequence in fragments shorter than     852 bp
  45% of sequence in fragments shorter than     945 bp
  50% of sequence in fragments shorter than    1046 bp
  55% of sequence in fragments shorter than    1156 bp
  60% of sequence in fragments shorter than    1278 bp
  65% of sequence in fragments shorter than    1412 bp
  70% of sequence in fragments shorter than    1566 bp
  75% of sequence in fragments shorter than    1743 bp
  80% of sequence in fragments shorter than    1951 bp
  85% of sequence in fragments shorter than    2221 bp
  90% of sequence in fragments shorter than    2620 bp
  95% of sequence in fragments shorter than    3346 bp

# AciI.BciVI (CCGC+GTATCC) double digest, 4901 thousand fragments (4206130+696093 sites), 607 bp average fragment length
   5% of sequence in fragments shorter than     196 bp
  10% of sequence in fragments shorter than     355 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     650 bp
  25% of sequence in fragments shorter than     796 bp
  30% of sequence in fragments shorter than     940 bp
  35% of sequence in fragments shorter than    1087 bp
  40% of sequence in fragments shorter than    1245 bp
  45% of sequence in fragments shorter than    1409 bp
  50% of sequence in fragments shorter than    1591 bp
  55% of sequence in fragments shorter than    1789 bp
  60% of sequence in fragments shorter than    2012 bp
  65% of sequence in fragments shorter than    2260 bp
  70% of sequence in fragments shorter than    2547 bp
  75% of sequence in fragments shorter than    2882 bp
  80% of sequence in fragments shorter than    3292 bp
  85% of sequence in fragments shorter than    3811 bp
  90% of sequence in fragments shorter than    4546 bp
  95% of sequence in fragments shorter than    5840 bp

# BscAI.BscGI (GCATC+CCCGT) double digest, 4883 thousand fragments (3652926+1231070 sites), 610 bp average fragment length
   5% of sequence in fragments shorter than     205 bp
  10% of sequence in fragments shorter than     320 bp
  15% of sequence in fragments shorter than     405 bp
  20% of sequence in fragments shorter than     504 bp
  25% of sequence in fragments shorter than     594 bp
  30% of sequence in fragments shorter than     687 bp
  35% of sequence in fragments shorter than     780 bp
  40% of sequence in fragments shorter than     879 bp
  45% of sequence in fragments shorter than     986 bp
  50% of sequence in fragments shorter than    1100 bp
  55% of sequence in fragments shorter than    1219 bp
  60% of sequence in fragments shorter than    1349 bp
  65% of sequence in fragments shorter than    1494 bp
  70% of sequence in fragments shorter than    1652 bp
  75% of sequence in fragments shorter than    1841 bp
  80% of sequence in fragments shorter than    2068 bp
  85% of sequence in fragments shorter than    2356 bp
  90% of sequence in fragments shorter than    2756 bp
  95% of sequence in fragments shorter than    3428 bp

# HindIII.Tsp45I (AAGCTT+GTSAC) double digest, 4881 thousand fragments (860365+4021761 sites), 610 bp average fragment length
   5% of sequence in fragments shorter than     212 bp
  10% of sequence in fragments shorter than     323 bp
  15% of sequence in fragments shorter than     411 bp
  20% of sequence in fragments shorter than     502 bp
  25% of sequence in fragments shorter than     588 bp
  30% of sequence in fragments shorter than     677 bp
  35% of sequence in fragments shorter than     764 bp
  40% of sequence in fragments shorter than     858 bp
  45% of sequence in fragments shorter than     953 bp
  50% of sequence in fragments shorter than    1055 bp
  55% of sequence in fragments shorter than    1166 bp
  60% of sequence in fragments shorter than    1288 bp
  65% of sequence in fragments shorter than    1418 bp
  70% of sequence in fragments shorter than    1574 bp
  75% of sequence in fragments shorter than    1755 bp
  80% of sequence in fragments shorter than    1969 bp
  85% of sequence in fragments shorter than    2251 bp
  90% of sequence in fragments shorter than    2648 bp
  95% of sequence in fragments shorter than    3328 bp

# Tsp45I.XbaI (GTSAC+TCTAGA) double digest, 4872 thousand fragments (4021761+850998 sites), 611 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     323 bp
  15% of sequence in fragments shorter than     415 bp
  20% of sequence in fragments shorter than     505 bp
  25% of sequence in fragments shorter than     592 bp
  30% of sequence in fragments shorter than     679 bp
  35% of sequence in fragments shorter than     768 bp
  40% of sequence in fragments shorter than     858 bp
  45% of sequence in fragments shorter than     949 bp
  50% of sequence in fragments shorter than    1050 bp
  55% of sequence in fragments shorter than    1161 bp
  60% of sequence in fragments shorter than    1279 bp
  65% of sequence in fragments shorter than    1402 bp
  70% of sequence in fragments shorter than    1548 bp
  75% of sequence in fragments shorter than    1720 bp
  80% of sequence in fragments shorter than    1927 bp
  85% of sequence in fragments shorter than    2194 bp
  90% of sequence in fragments shorter than    2556 bp
  95% of sequence in fragments shorter than    3204 bp

# HhaI.StyI (GCGC+CCWWGG) double digest, 4870 thousand fragments (1756502+3114107 sites), 611 bp average fragment length
   5% of sequence in fragments shorter than     199 bp
  10% of sequence in fragments shorter than     323 bp
  15% of sequence in fragments shorter than     429 bp
  20% of sequence in fragments shorter than     534 bp
  25% of sequence in fragments shorter than     643 bp
  30% of sequence in fragments shorter than     743 bp
  35% of sequence in fragments shorter than     852 bp
  40% of sequence in fragments shorter than     970 bp
  45% of sequence in fragments shorter than    1094 bp
  50% of sequence in fragments shorter than    1232 bp
  55% of sequence in fragments shorter than    1380 bp
  60% of sequence in fragments shorter than    1546 bp
  65% of sequence in fragments shorter than    1731 bp
  70% of sequence in fragments shorter than    1941 bp
  75% of sequence in fragments shorter than    2193 bp
  80% of sequence in fragments shorter than    2495 bp
  85% of sequence in fragments shorter than    2881 bp
  90% of sequence in fragments shorter than    3400 bp
  95% of sequence in fragments shorter than    4329 bp

# EcoRI.Tsp45I (GAATTC+GTSAC) double digest, 4868 thousand fragments (847401+4021761 sites), 612 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     325 bp
  15% of sequence in fragments shorter than     410 bp
  20% of sequence in fragments shorter than     500 bp
  25% of sequence in fragments shorter than     586 bp
  30% of sequence in fragments shorter than     676 bp
  35% of sequence in fragments shorter than     759 bp
  40% of sequence in fragments shorter than     853 bp
  45% of sequence in fragments shorter than     948 bp
  50% of sequence in fragments shorter than    1052 bp
  55% of sequence in fragments shorter than    1162 bp
  60% of sequence in fragments shorter than    1283 bp
  65% of sequence in fragments shorter than    1418 bp
  70% of sequence in fragments shorter than    1573 bp
  75% of sequence in fragments shorter than    1748 bp
  80% of sequence in fragments shorter than    1964 bp
  85% of sequence in fragments shorter than    2245 bp
  90% of sequence in fragments shorter than    2641 bp
  95% of sequence in fragments shorter than    3299 bp

# AvaIII.MlyI (ATGCAT+GAGTC) double digest, 4864 thousand fragments (933357+3931692 sites), 612 bp average fragment length
   5% of sequence in fragments shorter than     216 bp
  10% of sequence in fragments shorter than     321 bp
  15% of sequence in fragments shorter than     412 bp
  20% of sequence in fragments shorter than     499 bp
  25% of sequence in fragments shorter than     584 bp
  30% of sequence in fragments shorter than     669 bp
  35% of sequence in fragments shorter than     756 bp
  40% of sequence in fragments shorter than     846 bp
  45% of sequence in fragments shorter than     939 bp
  50% of sequence in fragments shorter than    1039 bp
  55% of sequence in fragments shorter than    1148 bp
  60% of sequence in fragments shorter than    1269 bp
  65% of sequence in fragments shorter than    1401 bp
  70% of sequence in fragments shorter than    1554 bp
  75% of sequence in fragments shorter than    1732 bp
  80% of sequence in fragments shorter than    1949 bp
  85% of sequence in fragments shorter than    2233 bp
  90% of sequence in fragments shorter than    2660 bp
  95% of sequence in fragments shorter than    3430 bp

# HhaI.NspI (GCGC+RCATGY) double digest, 4860 thousand fragments (1756502+3104359 sites), 613 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     338 bp
  15% of sequence in fragments shorter than     441 bp
  20% of sequence in fragments shorter than     547 bp
  25% of sequence in fragments shorter than     651 bp
  30% of sequence in fragments shorter than     745 bp
  35% of sequence in fragments shorter than     850 bp
  40% of sequence in fragments shorter than     956 bp
  45% of sequence in fragments shorter than    1067 bp
  50% of sequence in fragments shorter than    1188 bp
  55% of sequence in fragments shorter than    1316 bp
  60% of sequence in fragments shorter than    1455 bp
  65% of sequence in fragments shorter than    1612 bp
  70% of sequence in fragments shorter than    1786 bp
  75% of sequence in fragments shorter than    1991 bp
  80% of sequence in fragments shorter than    2241 bp
  85% of sequence in fragments shorter than    2562 bp
  90% of sequence in fragments shorter than    3011 bp
  95% of sequence in fragments shorter than    3740 bp

# MlyI.StuI (GAGTC+AGGCCT) double digest, 4856 thousand fragments (3931692+925729 sites), 613 bp average fragment length
   5% of sequence in fragments shorter than     213 bp
  10% of sequence in fragments shorter than     320 bp
  15% of sequence in fragments shorter than     406 bp
  20% of sequence in fragments shorter than     500 bp
  25% of sequence in fragments shorter than     589 bp
  30% of sequence in fragments shorter than     681 bp
  35% of sequence in fragments shorter than     765 bp
  40% of sequence in fragments shorter than     862 bp
  45% of sequence in fragments shorter than     965 bp
  50% of sequence in fragments shorter than    1073 bp
  55% of sequence in fragments shorter than    1193 bp
  60% of sequence in fragments shorter than    1324 bp
  65% of sequence in fragments shorter than    1456 bp
  70% of sequence in fragments shorter than    1620 bp
  75% of sequence in fragments shorter than    1813 bp
  80% of sequence in fragments shorter than    2051 bp
  85% of sequence in fragments shorter than    2360 bp
  90% of sequence in fragments shorter than    2783 bp
  95% of sequence in fragments shorter than    3500 bp

# AciI.AflII (CCGC+CTTAAG) double digest, 4848 thousand fragments (4206130+643284 sites), 614 bp average fragment length
   5% of sequence in fragments shorter than     200 bp
  10% of sequence in fragments shorter than     363 bp
  15% of sequence in fragments shorter than     513 bp
  20% of sequence in fragments shorter than     659 bp
  25% of sequence in fragments shorter than     806 bp
  30% of sequence in fragments shorter than     952 bp
  35% of sequence in fragments shorter than    1098 bp
  40% of sequence in fragments shorter than    1261 bp
  45% of sequence in fragments shorter than    1432 bp
  50% of sequence in fragments shorter than    1613 bp
  55% of sequence in fragments shorter than    1814 bp
  60% of sequence in fragments shorter than    2041 bp
  65% of sequence in fragments shorter than    2293 bp
  70% of sequence in fragments shorter than    2583 bp
  75% of sequence in fragments shorter than    2926 bp
  80% of sequence in fragments shorter than    3344 bp
  85% of sequence in fragments shorter than    3873 bp
  90% of sequence in fragments shorter than    4640 bp
  95% of sequence in fragments shorter than    5960 bp

# AciI.HaeII (CCGC+RGCGCY) double digest, 4835 thousand fragments (4206130+630101 sites), 616 bp average fragment length
   5% of sequence in fragments shorter than     193 bp
  10% of sequence in fragments shorter than     362 bp
  15% of sequence in fragments shorter than     539 bp
  20% of sequence in fragments shorter than     707 bp
  25% of sequence in fragments shorter than     883 bp
  30% of sequence in fragments shorter than    1073 bp
  35% of sequence in fragments shorter than    1274 bp
  40% of sequence in fragments shorter than    1489 bp
  45% of sequence in fragments shorter than    1726 bp
  50% of sequence in fragments shorter than    1988 bp
  55% of sequence in fragments shorter than    2276 bp
  60% of sequence in fragments shorter than    2599 bp
  65% of sequence in fragments shorter than    2960 bp
  70% of sequence in fragments shorter than    3388 bp
  75% of sequence in fragments shorter than    3895 bp
  80% of sequence in fragments shorter than    4507 bp
  85% of sequence in fragments shorter than    5295 bp
  90% of sequence in fragments shorter than    6431 bp
  95% of sequence in fragments shorter than    8373 bp

# MlyI.NdeI (GAGTC+CATATG) double digest, 4834 thousand fragments (3931692+903360 sites), 616 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     323 bp
  15% of sequence in fragments shorter than     415 bp
  20% of sequence in fragments shorter than     502 bp
  25% of sequence in fragments shorter than     588 bp
  30% of sequence in fragments shorter than     674 bp
  35% of sequence in fragments shorter than     759 bp
  40% of sequence in fragments shorter than     846 bp
  45% of sequence in fragments shorter than     938 bp
  50% of sequence in fragments shorter than    1036 bp
  55% of sequence in fragments shorter than    1140 bp
  60% of sequence in fragments shorter than    1249 bp
  65% of sequence in fragments shorter than    1376 bp
  70% of sequence in fragments shorter than    1518 bp
  75% of sequence in fragments shorter than    1686 bp
  80% of sequence in fragments shorter than    1884 bp
  85% of sequence in fragments shorter than    2148 bp
  90% of sequence in fragments shorter than    2523 bp
  95% of sequence in fragments shorter than    3237 bp

# BfiI.StyI (ACTGGG+CCWWGG) double digest, 4820 thousand fragments (1706594+3114107 sites), 618 bp average fragment length
   5% of sequence in fragments shorter than     202 bp
  10% of sequence in fragments shorter than     316 bp
  15% of sequence in fragments shorter than     417 bp
  20% of sequence in fragments shorter than     510 bp
  25% of sequence in fragments shorter than     601 bp
  30% of sequence in fragments shorter than     695 bp
  35% of sequence in fragments shorter than     794 bp
  40% of sequence in fragments shorter than     900 bp
  45% of sequence in fragments shorter than    1012 bp
  50% of sequence in fragments shorter than    1129 bp
  55% of sequence in fragments shorter than    1257 bp
  60% of sequence in fragments shorter than    1397 bp
  65% of sequence in fragments shorter than    1550 bp
  70% of sequence in fragments shorter than    1725 bp
  75% of sequence in fragments shorter than    1940 bp
  80% of sequence in fragments shorter than    2202 bp
  85% of sequence in fragments shorter than    2545 bp
  90% of sequence in fragments shorter than    3043 bp
  95% of sequence in fragments shorter than    3958 bp

# BfiI.NspI (ACTGGG+RCATGY) double digest, 4810 thousand fragments (1706594+3104359 sites), 619 bp average fragment length
   5% of sequence in fragments shorter than     217 bp
  10% of sequence in fragments shorter than     327 bp
  15% of sequence in fragments shorter than     425 bp
  20% of sequence in fragments shorter than     521 bp
  25% of sequence in fragments shorter than     613 bp
  30% of sequence in fragments shorter than     703 bp
  35% of sequence in fragments shorter than     797 bp
  40% of sequence in fragments shorter than     894 bp
  45% of sequence in fragments shorter than     996 bp
  50% of sequence in fragments shorter than    1102 bp
  55% of sequence in fragments shorter than    1218 bp
  60% of sequence in fragments shorter than    1345 bp
  65% of sequence in fragments shorter than    1486 bp
  70% of sequence in fragments shorter than    1645 bp
  75% of sequence in fragments shorter than    1828 bp
  80% of sequence in fragments shorter than    2051 bp
  85% of sequence in fragments shorter than    2329 bp
  90% of sequence in fragments shorter than    2745 bp
  95% of sequence in fragments shorter than    3431 bp

# AciI.SacI (CCGC+GAGCTC) double digest, 4808 thousand fragments (4206130+603323 sites), 619 bp average fragment length
   5% of sequence in fragments shorter than     195 bp
  10% of sequence in fragments shorter than     362 bp
  15% of sequence in fragments shorter than     522 bp
  20% of sequence in fragments shorter than     678 bp
  25% of sequence in fragments shorter than     836 bp
  30% of sequence in fragments shorter than     996 bp
  35% of sequence in fragments shorter than    1162 bp
  40% of sequence in fragments shorter than    1344 bp
  45% of sequence in fragments shorter than    1533 bp
  50% of sequence in fragments shorter than    1752 bp
  55% of sequence in fragments shorter than    1996 bp
  60% of sequence in fragments shorter than    2264 bp
  65% of sequence in fragments shorter than    2569 bp
  70% of sequence in fragments shorter than    2913 bp
  75% of sequence in fragments shorter than    3331 bp
  80% of sequence in fragments shorter than    3845 bp
  85% of sequence in fragments shorter than    4503 bp
  90% of sequence in fragments shorter than    5442 bp
  95% of sequence in fragments shorter than    7078 bp

# AciI.AvrII (CCGC+CCTAGG) double digest, 4800 thousand fragments (4206130+594956 sites), 620 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     361 bp
  15% of sequence in fragments shorter than     519 bp
  20% of sequence in fragments shorter than     667 bp
  25% of sequence in fragments shorter than     825 bp
  30% of sequence in fragments shorter than     981 bp
  35% of sequence in fragments shorter than    1150 bp
  40% of sequence in fragments shorter than    1328 bp
  45% of sequence in fragments shorter than    1517 bp
  50% of sequence in fragments shorter than    1725 bp
  55% of sequence in fragments shorter than    1956 bp
  60% of sequence in fragments shorter than    2217 bp
  65% of sequence in fragments shorter than    2505 bp
  70% of sequence in fragments shorter than    2839 bp
  75% of sequence in fragments shorter than    3240 bp
  80% of sequence in fragments shorter than    3724 bp
  85% of sequence in fragments shorter than    4349 bp
  90% of sequence in fragments shorter than    5240 bp
  95% of sequence in fragments shorter than    6790 bp

# BglII.Tsp45I (AGATCT+GTSAC) double digest, 4796 thousand fragments (774732+4021761 sites), 621 bp average fragment length
   5% of sequence in fragments shorter than     217 bp
  10% of sequence in fragments shorter than     329 bp
  15% of sequence in fragments shorter than     417 bp
  20% of sequence in fragments shorter than     508 bp
  25% of sequence in fragments shorter than     597 bp
  30% of sequence in fragments shorter than     687 bp
  35% of sequence in fragments shorter than     776 bp
  40% of sequence in fragments shorter than     868 bp
  45% of sequence in fragments shorter than     968 bp
  50% of sequence in fragments shorter than    1072 bp
  55% of sequence in fragments shorter than    1184 bp
  60% of sequence in fragments shorter than    1308 bp
  65% of sequence in fragments shorter than    1445 bp
  70% of sequence in fragments shorter than    1606 bp
  75% of sequence in fragments shorter than    1791 bp
  80% of sequence in fragments shorter than    2018 bp
  85% of sequence in fragments shorter than    2313 bp
  90% of sequence in fragments shorter than    2745 bp
  95% of sequence in fragments shorter than    3537 bp

# HindIII.MlyI (AAGCTT+GAGTC) double digest, 4791 thousand fragments (860365+3931692 sites), 621 bp average fragment length
   5% of sequence in fragments shorter than     218 bp
  10% of sequence in fragments shorter than     323 bp
  15% of sequence in fragments shorter than     417 bp
  20% of sequence in fragments shorter than     506 bp
  25% of sequence in fragments shorter than     593 bp
  30% of sequence in fragments shorter than     680 bp
  35% of sequence in fragments shorter than     769 bp
  40% of sequence in fragments shorter than     861 bp
  45% of sequence in fragments shorter than     957 bp
  50% of sequence in fragments shorter than    1059 bp
  55% of sequence in fragments shorter than    1169 bp
  60% of sequence in fragments shorter than    1290 bp
  65% of sequence in fragments shorter than    1422 bp
  70% of sequence in fragments shorter than    1576 bp
  75% of sequence in fragments shorter than    1760 bp
  80% of sequence in fragments shorter than    1982 bp
  85% of sequence in fragments shorter than    2280 bp
  90% of sequence in fragments shorter than    2696 bp
  95% of sequence in fragments shorter than    3434 bp

# MlyI.XbaI (GAGTC+TCTAGA) double digest, 4782 thousand fragments (3931692+850998 sites), 623 bp average fragment length
   5% of sequence in fragments shorter than     221 bp
  10% of sequence in fragments shorter than     331 bp
  15% of sequence in fragments shorter than     424 bp
  20% of sequence in fragments shorter than     515 bp
  25% of sequence in fragments shorter than     604 bp
  30% of sequence in fragments shorter than     690 bp
  35% of sequence in fragments shorter than     775 bp
  40% of sequence in fragments shorter than     866 bp
  45% of sequence in fragments shorter than     959 bp
  50% of sequence in fragments shorter than    1060 bp
  55% of sequence in fragments shorter than    1171 bp
  60% of sequence in fragments shorter than    1292 bp
  65% of sequence in fragments shorter than    1415 bp
  70% of sequence in fragments shorter than    1566 bp
  75% of sequence in fragments shorter than    1734 bp
  80% of sequence in fragments shorter than    1939 bp
  85% of sequence in fragments shorter than    2204 bp
  90% of sequence in fragments shorter than    2577 bp
  95% of sequence in fragments shorter than    3226 bp

# NcoI.Tsp45I (CCATGG+GTSAC) double digest, 4780 thousand fragments (759594+4021761 sites), 623 bp average fragment length
   5% of sequence in fragments shorter than     216 bp
  10% of sequence in fragments shorter than     327 bp
  15% of sequence in fragments shorter than     421 bp
  20% of sequence in fragments shorter than     513 bp
  25% of sequence in fragments shorter than     604 bp
  30% of sequence in fragments shorter than     694 bp
  35% of sequence in fragments shorter than     788 bp
  40% of sequence in fragments shorter than     883 bp
  45% of sequence in fragments shorter than     986 bp
  50% of sequence in fragments shorter than    1095 bp
  55% of sequence in fragments shorter than    1213 bp
  60% of sequence in fragments shorter than    1342 bp
  65% of sequence in fragments shorter than    1487 bp
  70% of sequence in fragments shorter than    1652 bp
  75% of sequence in fragments shorter than    1847 bp
  80% of sequence in fragments shorter than    2084 bp
  85% of sequence in fragments shorter than    2394 bp
  90% of sequence in fragments shorter than    2845 bp
  95% of sequence in fragments shorter than    3603 bp

# EcoRI.MlyI (GAATTC+GAGTC) double digest, 4778 thousand fragments (847401+3931692 sites), 623 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     327 bp
  15% of sequence in fragments shorter than     417 bp
  20% of sequence in fragments shorter than     508 bp
  25% of sequence in fragments shorter than     597 bp
  30% of sequence in fragments shorter than     685 bp
  35% of sequence in fragments shorter than     770 bp
  40% of sequence in fragments shorter than     862 bp
  45% of sequence in fragments shorter than     960 bp
  50% of sequence in fragments shorter than    1061 bp
  55% of sequence in fragments shorter than    1174 bp
  60% of sequence in fragments shorter than    1299 bp
  65% of sequence in fragments shorter than    1433 bp
  70% of sequence in fragments shorter than    1591 bp
  75% of sequence in fragments shorter than    1772 bp
  80% of sequence in fragments shorter than    1994 bp
  85% of sequence in fragments shorter than    2286 bp
  90% of sequence in fragments shorter than    2709 bp
  95% of sequence in fragments shorter than    3408 bp

# AciI.HgaI (CCGC+GACGC) double digest, 4777 thousand fragments (4206130+571889 sites), 623 bp average fragment length
   5% of sequence in fragments shorter than     195 bp
  10% of sequence in fragments shorter than     372 bp
  15% of sequence in fragments shorter than     544 bp
  20% of sequence in fragments shorter than     715 bp
  25% of sequence in fragments shorter than     884 bp
  30% of sequence in fragments shorter than    1065 bp
  35% of sequence in fragments shorter than    1257 bp
  40% of sequence in fragments shorter than    1456 bp
  45% of sequence in fragments shorter than    1679 bp
  50% of sequence in fragments shorter than    1923 bp
  55% of sequence in fragments shorter than    2195 bp
  60% of sequence in fragments shorter than    2494 bp
  65% of sequence in fragments shorter than    2832 bp
  70% of sequence in fragments shorter than    3230 bp
  75% of sequence in fragments shorter than    3705 bp
  80% of sequence in fragments shorter than    4282 bp
  85% of sequence in fragments shorter than    5031 bp
  90% of sequence in fragments shorter than    6091 bp
  95% of sequence in fragments shorter than    7895 bp

# AciI.EciI (CCGC+GGCGGA) double digest, 4776 thousand fragments (4206130+571273 sites), 623 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     628 bp
  20% of sequence in fragments shorter than     826 bp
  25% of sequence in fragments shorter than    1022 bp
  30% of sequence in fragments shorter than    1227 bp
  35% of sequence in fragments shorter than    1444 bp
  40% of sequence in fragments shorter than    1680 bp
  45% of sequence in fragments shorter than    1938 bp
  50% of sequence in fragments shorter than    2224 bp
  55% of sequence in fragments shorter than    2538 bp
  60% of sequence in fragments shorter than    2888 bp
  65% of sequence in fragments shorter than    3289 bp
  70% of sequence in fragments shorter than    3757 bp
  75% of sequence in fragments shorter than    4309 bp
  80% of sequence in fragments shorter than    4987 bp
  85% of sequence in fragments shorter than    5863 bp
  90% of sequence in fragments shorter than    7098 bp
  95% of sequence in fragments shorter than    9264 bp

# AciI.MfeI (CCGC+CAATTG) double digest, 4769 thousand fragments (4206130+564303 sites), 624 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     369 bp
  15% of sequence in fragments shorter than     525 bp
  20% of sequence in fragments shorter than     676 bp
  25% of sequence in fragments shorter than     825 bp
  30% of sequence in fragments shorter than     976 bp
  35% of sequence in fragments shorter than    1132 bp
  40% of sequence in fragments shorter than    1296 bp
  45% of sequence in fragments shorter than    1467 bp
  50% of sequence in fragments shorter than    1651 bp
  55% of sequence in fragments shorter than    1858 bp
  60% of sequence in fragments shorter than    2085 bp
  65% of sequence in fragments shorter than    2339 bp
  70% of sequence in fragments shorter than    2631 bp
  75% of sequence in fragments shorter than    2975 bp
  80% of sequence in fragments shorter than    3394 bp
  85% of sequence in fragments shorter than    3926 bp
  90% of sequence in fragments shorter than    4670 bp
  95% of sequence in fragments shorter than    5952 bp

# BscGI.SfeI (CCCGT+CTRYAG) double digest, 4761 thousand fragments (1231070+3531009 sites), 625 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     330 bp
  15% of sequence in fragments shorter than     415 bp
  20% of sequence in fragments shorter than     510 bp
  25% of sequence in fragments shorter than     607 bp
  30% of sequence in fragments shorter than     700 bp
  35% of sequence in fragments shorter than     800 bp
  40% of sequence in fragments shorter than     902 bp
  45% of sequence in fragments shorter than    1011 bp
  50% of sequence in fragments shorter than    1121 bp
  55% of sequence in fragments shorter than    1246 bp
  60% of sequence in fragments shorter than    1382 bp
  65% of sequence in fragments shorter than    1533 bp
  70% of sequence in fragments shorter than    1702 bp
  75% of sequence in fragments shorter than    1899 bp
  80% of sequence in fragments shorter than    2129 bp
  85% of sequence in fragments shorter than    2414 bp
  90% of sequence in fragments shorter than    2829 bp
  95% of sequence in fragments shorter than    3529 bp

# PsiI.StyI (TTATAA+CCWWGG) double digest, 4761 thousand fragments (1647912+3114107 sites), 625 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     329 bp
  15% of sequence in fragments shorter than     425 bp
  20% of sequence in fragments shorter than     514 bp
  25% of sequence in fragments shorter than     604 bp
  30% of sequence in fragments shorter than     688 bp
  35% of sequence in fragments shorter than     775 bp
  40% of sequence in fragments shorter than     869 bp
  45% of sequence in fragments shorter than     967 bp
  50% of sequence in fragments shorter than    1070 bp
  55% of sequence in fragments shorter than    1182 bp
  60% of sequence in fragments shorter than    1303 bp
  65% of sequence in fragments shorter than    1433 bp
  70% of sequence in fragments shorter than    1580 bp
  75% of sequence in fragments shorter than    1748 bp
  80% of sequence in fragments shorter than    1960 bp
  85% of sequence in fragments shorter than    2237 bp
  90% of sequence in fragments shorter than    2635 bp
  95% of sequence in fragments shorter than    3364 bp

# BclI.Tsp45I (TGATCA+GTSAC) double digest, 4760 thousand fragments (738785+4021761 sites), 626 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     332 bp
  15% of sequence in fragments shorter than     425 bp
  20% of sequence in fragments shorter than     517 bp
  25% of sequence in fragments shorter than     606 bp
  30% of sequence in fragments shorter than     694 bp
  35% of sequence in fragments shorter than     786 bp
  40% of sequence in fragments shorter than     877 bp
  45% of sequence in fragments shorter than     977 bp
  50% of sequence in fragments shorter than    1082 bp
  55% of sequence in fragments shorter than    1198 bp
  60% of sequence in fragments shorter than    1319 bp
  65% of sequence in fragments shorter than    1457 bp
  70% of sequence in fragments shorter than    1616 bp
  75% of sequence in fragments shorter than    1798 bp
  80% of sequence in fragments shorter than    2018 bp
  85% of sequence in fragments shorter than    2305 bp
  90% of sequence in fragments shorter than    2706 bp
  95% of sequence in fragments shorter than    3421 bp

# AciI.SphI (CCGC+GCATGC) double digest, 4756 thousand fragments (4206130+550952 sites), 626 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     370 bp
  15% of sequence in fragments shorter than     533 bp
  20% of sequence in fragments shorter than     691 bp
  25% of sequence in fragments shorter than     852 bp
  30% of sequence in fragments shorter than    1017 bp
  35% of sequence in fragments shorter than    1188 bp
  40% of sequence in fragments shorter than    1374 bp
  45% of sequence in fragments shorter than    1566 bp
  50% of sequence in fragments shorter than    1787 bp
  55% of sequence in fragments shorter than    2026 bp
  60% of sequence in fragments shorter than    2293 bp
  65% of sequence in fragments shorter than    2597 bp
  70% of sequence in fragments shorter than    2945 bp
  75% of sequence in fragments shorter than    3363 bp
  80% of sequence in fragments shorter than    3872 bp
  85% of sequence in fragments shorter than    4525 bp
  90% of sequence in fragments shorter than    5450 bp
  95% of sequence in fragments shorter than    7056 bp

# FnuDII.Tsp45I (CGCG+GTSAC) double digest, 4755 thousand fragments (733940+4021761 sites), 626 bp average fragment length
   5% of sequence in fragments shorter than     218 bp
  10% of sequence in fragments shorter than     335 bp
  15% of sequence in fragments shorter than     434 bp
  20% of sequence in fragments shorter than     535 bp
  25% of sequence in fragments shorter than     632 bp
  30% of sequence in fragments shorter than     729 bp
  35% of sequence in fragments shorter than     831 bp
  40% of sequence in fragments shorter than     935 bp
  45% of sequence in fragments shorter than    1045 bp
  50% of sequence in fragments shorter than    1165 bp
  55% of sequence in fragments shorter than    1294 bp
  60% of sequence in fragments shorter than    1436 bp
  65% of sequence in fragments shorter than    1599 bp
  70% of sequence in fragments shorter than    1783 bp
  75% of sequence in fragments shorter than    2002 bp
  80% of sequence in fragments shorter than    2272 bp
  85% of sequence in fragments shorter than    2631 bp
  90% of sequence in fragments shorter than    3160 bp
  95% of sequence in fragments shorter than    4129 bp

# AccI.BscAI (GTMKAC+GCATC) double digest, 4753 thousand fragments (1101063+3652926 sites), 626 bp average fragment length
   5% of sequence in fragments shorter than     214 bp
  10% of sequence in fragments shorter than     330 bp
  15% of sequence in fragments shorter than     432 bp
  20% of sequence in fragments shorter than     528 bp
  25% of sequence in fragments shorter than     617 bp
  30% of sequence in fragments shorter than     707 bp
  35% of sequence in fragments shorter than     799 bp
  40% of sequence in fragments shorter than     896 bp
  45% of sequence in fragments shorter than     998 bp
  50% of sequence in fragments shorter than    1106 bp
  55% of sequence in fragments shorter than    1217 bp
  60% of sequence in fragments shorter than    1340 bp
  65% of sequence in fragments shorter than    1466 bp
  70% of sequence in fragments shorter than    1614 bp
  75% of sequence in fragments shorter than    1794 bp
  80% of sequence in fragments shorter than    2009 bp
  85% of sequence in fragments shorter than    2286 bp
  90% of sequence in fragments shorter than    2679 bp
  95% of sequence in fragments shorter than    3378 bp

# NspI.PsiI (RCATGY+TTATAA) double digest, 4751 thousand fragments (3104359+1647912 sites), 627 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     329 bp
  15% of sequence in fragments shorter than     431 bp
  20% of sequence in fragments shorter than     529 bp
  25% of sequence in fragments shorter than     624 bp
  30% of sequence in fragments shorter than     719 bp
  35% of sequence in fragments shorter than     817 bp
  40% of sequence in fragments shorter than     919 bp
  45% of sequence in fragments shorter than    1027 bp
  50% of sequence in fragments shorter than    1140 bp
  55% of sequence in fragments shorter than    1264 bp
  60% of sequence in fragments shorter than    1400 bp
  65% of sequence in fragments shorter than    1554 bp
  70% of sequence in fragments shorter than    1728 bp
  75% of sequence in fragments shorter than    1935 bp
  80% of sequence in fragments shorter than    2185 bp
  85% of sequence in fragments shorter than    2516 bp
  90% of sequence in fragments shorter than    2951 bp
  95% of sequence in fragments shorter than    3801 bp

# AciI.ScaI (CCGC+AGTACT) double digest, 4749 thousand fragments (4206130+543793 sites), 627 bp average fragment length
   5% of sequence in fragments shorter than     204 bp
  10% of sequence in fragments shorter than     372 bp
  15% of sequence in fragments shorter than     530 bp
  20% of sequence in fragments shorter than     683 bp
  25% of sequence in fragments shorter than     834 bp
  30% of sequence in fragments shorter than     985 bp
  35% of sequence in fragments shorter than    1142 bp
  40% of sequence in fragments shorter than    1311 bp
  45% of sequence in fragments shorter than    1484 bp
  50% of sequence in fragments shorter than    1680 bp
  55% of sequence in fragments shorter than    1891 bp
  60% of sequence in fragments shorter than    2123 bp
  65% of sequence in fragments shorter than    2390 bp
  70% of sequence in fragments shorter than    2690 bp
  75% of sequence in fragments shorter than    3044 bp
  80% of sequence in fragments shorter than    3478 bp
  85% of sequence in fragments shorter than    4036 bp
  90% of sequence in fragments shorter than    4834 bp
  95% of sequence in fragments shorter than    6191 bp

# BscAI.PpuMI (GCATC+RGGWCCY) double digest, 4737 thousand fragments (3652926+1085138 sites), 629 bp average fragment length
   5% of sequence in fragments shorter than     208 bp
  10% of sequence in fragments shorter than     324 bp
  15% of sequence in fragments shorter than     426 bp
  20% of sequence in fragments shorter than     527 bp
  25% of sequence in fragments shorter than     626 bp
  30% of sequence in fragments shorter than     725 bp
  35% of sequence in fragments shorter than     825 bp
  40% of sequence in fragments shorter than     933 bp
  45% of sequence in fragments shorter than    1046 bp
  50% of sequence in fragments shorter than    1166 bp
  55% of sequence in fragments shorter than    1293 bp
  60% of sequence in fragments shorter than    1431 bp
  65% of sequence in fragments shorter than    1587 bp
  70% of sequence in fragments shorter than    1761 bp
  75% of sequence in fragments shorter than    1969 bp
  80% of sequence in fragments shorter than    2225 bp
  85% of sequence in fragments shorter than    2551 bp
  90% of sequence in fragments shorter than    3017 bp
  95% of sequence in fragments shorter than    3881 bp

# BscAI.PvuII (GCATC+CAGCTG) double digest, 4736 thousand fragments (3652926+1084260 sites), 629 bp average fragment length
   5% of sequence in fragments shorter than     210 bp
  10% of sequence in fragments shorter than     328 bp
  15% of sequence in fragments shorter than     433 bp
  20% of sequence in fragments shorter than     533 bp
  25% of sequence in fragments shorter than     630 bp
  30% of sequence in fragments shorter than     725 bp
  35% of sequence in fragments shorter than     823 bp
  40% of sequence in fragments shorter than     927 bp
  45% of sequence in fragments shorter than    1039 bp
  50% of sequence in fragments shorter than    1156 bp
  55% of sequence in fragments shorter than    1278 bp
  60% of sequence in fragments shorter than    1411 bp
  65% of sequence in fragments shorter than    1561 bp
  70% of sequence in fragments shorter than    1728 bp
  75% of sequence in fragments shorter than    1930 bp
  80% of sequence in fragments shorter than    2176 bp
  85% of sequence in fragments shorter than    2488 bp
  90% of sequence in fragments shorter than    2933 bp
  95% of sequence in fragments shorter than    3735 bp

# BsaAI.Tsp45I (YACGTR+GTSAC) double digest, 4735 thousand fragments (713801+4021761 sites), 629 bp average fragment length
   5% of sequence in fragments shorter than     221 bp
  10% of sequence in fragments shorter than     335 bp
  15% of sequence in fragments shorter than     428 bp
  20% of sequence in fragments shorter than     523 bp
  25% of sequence in fragments shorter than     615 bp
  30% of sequence in fragments shorter than     705 bp
  35% of sequence in fragments shorter than     800 bp
  40% of sequence in fragments shorter than     896 bp
  45% of sequence in fragments shorter than     999 bp
  50% of sequence in fragments shorter than    1105 bp
  55% of sequence in fragments shorter than    1223 bp
  60% of sequence in fragments shorter than    1353 bp
  65% of sequence in fragments shorter than    1497 bp
  70% of sequence in fragments shorter than    1665 bp
  75% of sequence in fragments shorter than    1854 bp
  80% of sequence in fragments shorter than    2088 bp
  85% of sequence in fragments shorter than    2394 bp
  90% of sequence in fragments shorter than    2832 bp
  95% of sequence in fragments shorter than    3563 bp

# BceAI.Tsp45I (ACGGC+GTSAC) double digest, 4726 thousand fragments (705178+4021761 sites), 630 bp average fragment length
   5% of sequence in fragments shorter than     217 bp
  10% of sequence in fragments shorter than     332 bp
  15% of sequence in fragments shorter than     427 bp
  20% of sequence in fragments shorter than     522 bp
  25% of sequence in fragments shorter than     616 bp
  30% of sequence in fragments shorter than     709 bp
  35% of sequence in fragments shorter than     807 bp
  40% of sequence in fragments shorter than     907 bp
  45% of sequence in fragments shorter than    1014 bp
  50% of sequence in fragments shorter than    1129 bp
  55% of sequence in fragments shorter than    1252 bp
  60% of sequence in fragments shorter than    1389 bp
  65% of sequence in fragments shorter than    1546 bp
  70% of sequence in fragments shorter than    1723 bp
  75% of sequence in fragments shorter than    1933 bp
  80% of sequence in fragments shorter than    2193 bp
  85% of sequence in fragments shorter than    2532 bp
  90% of sequence in fragments shorter than    3031 bp
  95% of sequence in fragments shorter than    3943 bp

# BciVI.Tsp45I (GTATCC+GTSAC) double digest, 4717 thousand fragments (696093+4021761 sites), 631 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     334 bp
  15% of sequence in fragments shorter than     427 bp
  20% of sequence in fragments shorter than     520 bp
  25% of sequence in fragments shorter than     610 bp
  30% of sequence in fragments shorter than     699 bp
  35% of sequence in fragments shorter than     792 bp
  40% of sequence in fragments shorter than     887 bp
  45% of sequence in fragments shorter than     987 bp
  50% of sequence in fragments shorter than    1094 bp
  55% of sequence in fragments shorter than    1208 bp
  60% of sequence in fragments shorter than    1333 bp
  65% of sequence in fragments shorter than    1471 bp
  70% of sequence in fragments shorter than    1629 bp
  75% of sequence in fragments shorter than    1807 bp
  80% of sequence in fragments shorter than    2021 bp
  85% of sequence in fragments shorter than    2295 bp
  90% of sequence in fragments shorter than    2678 bp
  95% of sequence in fragments shorter than    3371 bp

# EsaBC3I.StyI (TCGA+CCWWGG) double digest, 4716 thousand fragments (1603346+3114107 sites), 631 bp average fragment length
   5% of sequence in fragments shorter than     217 bp
  10% of sequence in fragments shorter than     332 bp
  15% of sequence in fragments shorter than     431 bp
  20% of sequence in fragments shorter than     521 bp
  25% of sequence in fragments shorter than     616 bp
  30% of sequence in fragments shorter than     708 bp
  35% of sequence in fragments shorter than     798 bp
  40% of sequence in fragments shorter than     898 bp
  45% of sequence in fragments shorter than    1006 bp
  50% of sequence in fragments shorter than    1116 bp
  55% of sequence in fragments shorter than    1236 bp
  60% of sequence in fragments shorter than    1370 bp
  65% of sequence in fragments shorter than    1516 bp
  70% of sequence in fragments shorter than    1689 bp
  75% of sequence in fragments shorter than    1889 bp
  80% of sequence in fragments shorter than    2132 bp
  85% of sequence in fragments shorter than    2443 bp
  90% of sequence in fragments shorter than    2885 bp
  95% of sequence in fragments shorter than    3653 bp

# EsaBC3I.NspI (TCGA+RCATGY) double digest, 4707 thousand fragments (1603346+3104359 sites), 633 bp average fragment length
   5% of sequence in fragments shorter than     224 bp
  10% of sequence in fragments shorter than     338 bp
  15% of sequence in fragments shorter than     436 bp
  20% of sequence in fragments shorter than     530 bp
  25% of sequence in fragments shorter than     623 bp
  30% of sequence in fragments shorter than     715 bp
  35% of sequence in fragments shorter than     808 bp
  40% of sequence in fragments shorter than     903 bp
  45% of sequence in fragments shorter than    1005 bp
  50% of sequence in fragments shorter than    1110 bp
  55% of sequence in fragments shorter than    1223 bp
  60% of sequence in fragments shorter than    1348 bp
  65% of sequence in fragments shorter than    1480 bp
  70% of sequence in fragments shorter than    1633 bp
  75% of sequence in fragments shorter than    1809 bp
  80% of sequence in fragments shorter than    2020 bp
  85% of sequence in fragments shorter than    2291 bp
  90% of sequence in fragments shorter than    2660 bp
  95% of sequence in fragments shorter than    3306 bp

# BglII.MlyI (AGATCT+GAGTC) double digest, 4705 thousand fragments (774732+3931692 sites), 633 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     329 bp
  15% of sequence in fragments shorter than     426 bp
  20% of sequence in fragments shorter than     516 bp
  25% of sequence in fragments shorter than     604 bp
  30% of sequence in fragments shorter than     694 bp
  35% of sequence in fragments shorter than     786 bp
  40% of sequence in fragments shorter than     880 bp
  45% of sequence in fragments shorter than     981 bp
  50% of sequence in fragments shorter than    1088 bp
  55% of sequence in fragments shorter than    1204 bp
  60% of sequence in fragments shorter than    1332 bp
  65% of sequence in fragments shorter than    1472 bp
  70% of sequence in fragments shorter than    1632 bp
  75% of sequence in fragments shorter than    1823 bp
  80% of sequence in fragments shorter than    2062 bp
  85% of sequence in fragments shorter than    2379 bp
  90% of sequence in fragments shorter than    2846 bp
  95% of sequence in fragments shorter than    3697 bp

# AciI.ApaLI (CCGC+GTGCAC) double digest, 4701 thousand fragments (4206130+496255 sites), 633 bp average fragment length
   5% of sequence in fragments shorter than     199 bp
  10% of sequence in fragments shorter than     370 bp
  15% of sequence in fragments shorter than     536 bp
  20% of sequence in fragments shorter than     698 bp
  25% of sequence in fragments shorter than     863 bp
  30% of sequence in fragments shorter than    1033 bp
  35% of sequence in fragments shorter than    1212 bp
  40% of sequence in fragments shorter than    1399 bp
  45% of sequence in fragments shorter than    1604 bp
  50% of sequence in fragments shorter than    1834 bp
  55% of sequence in fragments shorter than    2082 bp
  60% of sequence in fragments shorter than    2363 bp
  65% of sequence in fragments shorter than    2676 bp
  70% of sequence in fragments shorter than    3040 bp
  75% of sequence in fragments shorter than    3471 bp
  80% of sequence in fragments shorter than    4011 bp
  85% of sequence in fragments shorter than    4695 bp
  90% of sequence in fragments shorter than    5659 bp
  95% of sequence in fragments shorter than    7333 bp

# HpaII.SspI (CCGG+AATATT) double digest, 4694 thousand fragments (2317722+2377267 sites), 634 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     334 bp
  15% of sequence in fragments shorter than     448 bp
  20% of sequence in fragments shorter than     555 bp
  25% of sequence in fragments shorter than     659 bp
  30% of sequence in fragments shorter than     763 bp
  35% of sequence in fragments shorter than     871 bp
  40% of sequence in fragments shorter than     984 bp
  45% of sequence in fragments shorter than    1100 bp
  50% of sequence in fragments shorter than    1226 bp
  55% of sequence in fragments shorter than    1362 bp
  60% of sequence in fragments shorter than    1504 bp
  65% of sequence in fragments shorter than    1666 bp
  70% of sequence in fragments shorter than    1855 bp
  75% of sequence in fragments shorter than    2073 bp
  80% of sequence in fragments shorter than    2348 bp
  85% of sequence in fragments shorter than    2698 bp
  90% of sequence in fragments shorter than    3202 bp
  95% of sequence in fragments shorter than    4144 bp

# MlyI.NcoI (GAGTC+CCATGG) double digest, 4690 thousand fragments (3931692+759594 sites), 635 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     329 bp
  15% of sequence in fragments shorter than     427 bp
  20% of sequence in fragments shorter than     520 bp
  25% of sequence in fragments shorter than     611 bp
  30% of sequence in fragments shorter than     702 bp
  35% of sequence in fragments shorter than     796 bp
  40% of sequence in fragments shorter than     891 bp
  45% of sequence in fragments shorter than     993 bp
  50% of sequence in fragments shorter than    1102 bp
  55% of sequence in fragments shorter than    1221 bp
  60% of sequence in fragments shorter than    1352 bp
  65% of sequence in fragments shorter than    1495 bp
  70% of sequence in fragments shorter than    1663 bp
  75% of sequence in fragments shorter than    1857 bp
  80% of sequence in fragments shorter than    2096 bp
  85% of sequence in fragments shorter than    2396 bp
  90% of sequence in fragments shorter than    2858 bp
  95% of sequence in fragments shorter than    3669 bp

# BclI.MlyI (TGATCA+GAGTC) double digest, 4669 thousand fragments (738785+3931692 sites), 638 bp average fragment length
   5% of sequence in fragments shorter than     222 bp
  10% of sequence in fragments shorter than     331 bp
  15% of sequence in fragments shorter than     428 bp
  20% of sequence in fragments shorter than     520 bp
  25% of sequence in fragments shorter than     610 bp
  30% of sequence in fragments shorter than     699 bp
  35% of sequence in fragments shorter than     789 bp
  40% of sequence in fragments shorter than     883 bp
  45% of sequence in fragments shorter than     984 bp
  50% of sequence in fragments shorter than    1088 bp
  55% of sequence in fragments shorter than    1202 bp
  60% of sequence in fragments shorter than    1327 bp
  65% of sequence in fragments shorter than    1468 bp
  70% of sequence in fragments shorter than    1627 bp
  75% of sequence in fragments shorter than    1813 bp
  80% of sequence in fragments shorter than    2047 bp
  85% of sequence in fragments shorter than    2359 bp
  90% of sequence in fragments shorter than    2800 bp
  95% of sequence in fragments shorter than    3627 bp

# AciI.ApaI (CCGC+GGGCCC) double digest, 4665 thousand fragments (4206130+460339 sites), 638 bp average fragment length
   5% of sequence in fragments shorter than     198 bp
  10% of sequence in fragments shorter than     376 bp
  15% of sequence in fragments shorter than     552 bp
  20% of sequence in fragments shorter than     726 bp
  25% of sequence in fragments shorter than     900 bp
  30% of sequence in fragments shorter than    1083 bp
  35% of sequence in fragments shorter than    1281 bp
  40% of sequence in fragments shorter than    1486 bp
  45% of sequence in fragments shorter than    1720 bp
  50% of sequence in fragments shorter than    1971 bp
  55% of sequence in fragments shorter than    2251 bp
  60% of sequence in fragments shorter than    2563 bp
  65% of sequence in fragments shorter than    2914 bp
  70% of sequence in fragments shorter than    3326 bp
  75% of sequence in fragments shorter than    3818 bp
  80% of sequence in fragments shorter than    4422 bp
  85% of sequence in fragments shorter than    5195 bp
  90% of sequence in fragments shorter than    6306 bp
  95% of sequence in fragments shorter than    8215 bp

# FnuDII.MlyI (CGCG+GAGTC) double digest, 4665 thousand fragments (733940+3931692 sites), 638 bp average fragment length
   5% of sequence in fragments shorter than     221 bp
  10% of sequence in fragments shorter than     332 bp
  15% of sequence in fragments shorter than     442 bp
  20% of sequence in fragments shorter than     547 bp
  25% of sequence in fragments shorter than     650 bp
  30% of sequence in fragments shorter than     752 bp
  35% of sequence in fragments shorter than     859 bp
  40% of sequence in fragments shorter than     968 bp
  45% of sequence in fragments shorter than    1083 bp
  50% of sequence in fragments shorter than    1209 bp
  55% of sequence in fragments shorter than    1346 bp
  60% of sequence in fragments shorter than    1496 bp
  65% of sequence in fragments shorter than    1666 bp
  70% of sequence in fragments shorter than    1857 bp
  75% of sequence in fragments shorter than    2086 bp
  80% of sequence in fragments shorter than    2377 bp
  85% of sequence in fragments shorter than    2765 bp
  90% of sequence in fragments shorter than    3314 bp
  95% of sequence in fragments shorter than    4351 bp

# AflII.Tsp45I (CTTAAG+GTSAC) double digest, 4664 thousand fragments (643284+4021761 sites), 638 bp average fragment length
   5% of sequence in fragments shorter than     224 bp
  10% of sequence in fragments shorter than     338 bp
  15% of sequence in fragments shorter than     431 bp
  20% of sequence in fragments shorter than     525 bp
  25% of sequence in fragments shorter than     616 bp
  30% of sequence in fragments shorter than     706 bp
  35% of sequence in fragments shorter than     801 bp
  40% of sequence in fragments shorter than     896 bp
  45% of sequence in fragments shorter than     999 bp
  50% of sequence in fragments shorter than    1107 bp
  55% of sequence in fragments shorter than    1224 bp
  60% of sequence in fragments shorter than    1352 bp
  65% of sequence in fragments shorter than    1495 bp
  70% of sequence in fragments shorter than    1658 bp
  75% of sequence in fragments shorter than    1841 bp
  80% of sequence in fragments shorter than    2069 bp
  85% of sequence in fragments shorter than    2349 bp
  90% of sequence in fragments shorter than    2757 bp
  95% of sequence in fragments shorter than    3493 bp

# HgiCI.StyI (GGYRCC+CCWWGG) double digest, 4664 thousand fragments (1550877+3114107 sites), 638 bp average fragment length
   5% of sequence in fragments shorter than     212 bp
  10% of sequence in fragments shorter than     326 bp
  15% of sequence in fragments shorter than     432 bp
  20% of sequence in fragments shorter than     534 bp
  25% of sequence in fragments shorter than     635 bp
  30% of sequence in fragments shorter than     737 bp
  35% of sequence in fragments shorter than     837 bp
  40% of sequence in fragments shorter than     945 bp
  45% of sequence in fragments shorter than    1062 bp
  50% of sequence in fragments shorter than    1192 bp
  55% of sequence in fragments shorter than    1333 bp
  60% of sequence in fragments shorter than    1485 bp
  65% of sequence in fragments shorter than    1653 bp
  70% of sequence in fragments shorter than    1856 bp
  75% of sequence in fragments shorter than    2093 bp
  80% of sequence in fragments shorter than    2384 bp
  85% of sequence in fragments shorter than    2769 bp
  90% of sequence in fragments shorter than    3337 bp
  95% of sequence in fragments shorter than    4458 bp

# BsmI.StyI (GAATGC+CCWWGG) double digest, 4662 thousand fragments (1549350+3114107 sites), 639 bp average fragment length
   5% of sequence in fragments shorter than     203 bp
  10% of sequence in fragments shorter than     325 bp
  15% of sequence in fragments shorter than     434 bp
  20% of sequence in fragments shorter than     534 bp
  25% of sequence in fragments shorter than     638 bp
  30% of sequence in fragments shorter than     739 bp
  35% of sequence in fragments shorter than     838 bp
  40% of sequence in fragments shorter than     947 bp
  45% of sequence in fragments shorter than    1058 bp
  50% of sequence in fragments shorter than    1177 bp
  55% of sequence in fragments shorter than    1306 bp
  60% of sequence in fragments shorter than    1446 bp
  65% of sequence in fragments shorter than    1603 bp
  70% of sequence in fragments shorter than    1777 bp
  75% of sequence in fragments shorter than    1986 bp
  80% of sequence in fragments shorter than    2235 bp
  85% of sequence in fragments shorter than    2541 bp
  90% of sequence in fragments shorter than    2967 bp
  95% of sequence in fragments shorter than    3700 bp

# AvaI.BscAI (CYCGRG+GCATC) double digest, 4658 thousand fragments (1005714+3652926 sites), 639 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     336 bp
  15% of sequence in fragments shorter than     438 bp
  20% of sequence in fragments shorter than     537 bp
  25% of sequence in fragments shorter than     632 bp
  30% of sequence in fragments shorter than     728 bp
  35% of sequence in fragments shorter than     825 bp
  40% of sequence in fragments shorter than     930 bp
  45% of sequence in fragments shorter than    1040 bp
  50% of sequence in fragments shorter than    1158 bp
  55% of sequence in fragments shorter than    1281 bp
  60% of sequence in fragments shorter than    1415 bp
  65% of sequence in fragments shorter than    1566 bp
  70% of sequence in fragments shorter than    1732 bp
  75% of sequence in fragments shorter than    1934 bp
  80% of sequence in fragments shorter than    2177 bp
  85% of sequence in fragments shorter than    2493 bp
  90% of sequence in fragments shorter than    2944 bp
  95% of sequence in fragments shorter than    3777 bp

# HgiCI.NspI (GGYRCC+RCATGY) double digest, 4654 thousand fragments (1550877+3104359 sites), 640 bp average fragment length
   5% of sequence in fragments shorter than     224 bp
  10% of sequence in fragments shorter than     336 bp
  15% of sequence in fragments shorter than     434 bp
  20% of sequence in fragments shorter than     528 bp
  25% of sequence in fragments shorter than     621 bp
  30% of sequence in fragments shorter than     713 bp
  35% of sequence in fragments shorter than     807 bp
  40% of sequence in fragments shorter than     905 bp
  45% of sequence in fragments shorter than    1000 bp
  50% of sequence in fragments shorter than    1108 bp
  55% of sequence in fragments shorter than    1219 bp
  60% of sequence in fragments shorter than    1339 bp
  65% of sequence in fragments shorter than    1477 bp
  70% of sequence in fragments shorter than    1630 bp
  75% of sequence in fragments shorter than    1815 bp
  80% of sequence in fragments shorter than    2042 bp
  85% of sequence in fragments shorter than    2321 bp
  90% of sequence in fragments shorter than    2739 bp
  95% of sequence in fragments shorter than    3503 bp

# BsmI.NspI (GAATGC+RCATGY) double digest, 4653 thousand fragments (1549350+3104359 sites), 640 bp average fragment length
   5% of sequence in fragments shorter than     208 bp
  10% of sequence in fragments shorter than     333 bp
  15% of sequence in fragments shorter than     442 bp
  20% of sequence in fragments shorter than     549 bp
  25% of sequence in fragments shorter than     653 bp
  30% of sequence in fragments shorter than     753 bp
  35% of sequence in fragments shorter than     856 bp
  40% of sequence in fragments shorter than     961 bp
  45% of sequence in fragments shorter than    1068 bp
  50% of sequence in fragments shorter than    1183 bp
  55% of sequence in fragments shorter than    1305 bp
  60% of sequence in fragments shorter than    1430 bp
  65% of sequence in fragments shorter than    1577 bp
  70% of sequence in fragments shorter than    1737 bp
  75% of sequence in fragments shorter than    1924 bp
  80% of sequence in fragments shorter than    2147 bp
  85% of sequence in fragments shorter than    2435 bp
  90% of sequence in fragments shorter than    2829 bp
  95% of sequence in fragments shorter than    3494 bp

# AciI.EcoRV (CCGC+GATATC) double digest, 4652 thousand fragments (4206130+446473 sites), 640 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     382 bp
  15% of sequence in fragments shorter than     544 bp
  20% of sequence in fragments shorter than     701 bp
  25% of sequence in fragments shorter than     857 bp
  30% of sequence in fragments shorter than    1021 bp
  35% of sequence in fragments shorter than    1187 bp
  40% of sequence in fragments shorter than    1363 bp
  45% of sequence in fragments shorter than    1543 bp
  50% of sequence in fragments shorter than    1749 bp
  55% of sequence in fragments shorter than    1973 bp
  60% of sequence in fragments shorter than    2219 bp
  65% of sequence in fragments shorter than    2491 bp
  70% of sequence in fragments shorter than    2805 bp
  75% of sequence in fragments shorter than    3181 bp
  80% of sequence in fragments shorter than    3634 bp
  85% of sequence in fragments shorter than    4217 bp
  90% of sequence in fragments shorter than    5033 bp
  95% of sequence in fragments shorter than    6439 bp

# HaeII.Tsp45I (RGCGCY+GTSAC) double digest, 4651 thousand fragments (630101+4021761 sites), 640 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     332 bp
  15% of sequence in fragments shorter than     428 bp
  20% of sequence in fragments shorter than     525 bp
  25% of sequence in fragments shorter than     620 bp
  30% of sequence in fragments shorter than     714 bp
  35% of sequence in fragments shorter than     815 bp
  40% of sequence in fragments shorter than     917 bp
  45% of sequence in fragments shorter than    1027 bp
  50% of sequence in fragments shorter than    1142 bp
  55% of sequence in fragments shorter than    1268 bp
  60% of sequence in fragments shorter than    1407 bp
  65% of sequence in fragments shorter than    1565 bp
  70% of sequence in fragments shorter than    1747 bp
  75% of sequence in fragments shorter than    1960 bp
  80% of sequence in fragments shorter than    2225 bp
  85% of sequence in fragments shorter than    2571 bp
  90% of sequence in fragments shorter than    3074 bp
  95% of sequence in fragments shorter than    3968 bp

# BsaAI.MlyI (YACGTR+GAGTC) double digest, 4645 thousand fragments (713801+3931692 sites), 641 bp average fragment length
   5% of sequence in fragments shorter than     224 bp
  10% of sequence in fragments shorter than     334 bp
  15% of sequence in fragments shorter than     432 bp
  20% of sequence in fragments shorter than     526 bp
  25% of sequence in fragments shorter than     618 bp
  30% of sequence in fragments shorter than     710 bp
  35% of sequence in fragments shorter than     802 bp
  40% of sequence in fragments shorter than     896 bp
  45% of sequence in fragments shorter than     998 bp
  50% of sequence in fragments shorter than    1103 bp
  55% of sequence in fragments shorter than    1221 bp
  60% of sequence in fragments shorter than    1351 bp
  65% of sequence in fragments shorter than    1493 bp
  70% of sequence in fragments shorter than    1659 bp
  75% of sequence in fragments shorter than    1850 bp
  80% of sequence in fragments shorter than    2083 bp
  85% of sequence in fragments shorter than    2378 bp
  90% of sequence in fragments shorter than    2807 bp
  95% of sequence in fragments shorter than    3576 bp

# BceAI.MlyI (ACGGC+GAGTC) double digest, 4636 thousand fragments (705178+3931692 sites), 642 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     330 bp
  15% of sequence in fragments shorter than     430 bp
  20% of sequence in fragments shorter than     526 bp
  25% of sequence in fragments shorter than     621 bp
  30% of sequence in fragments shorter than     716 bp
  35% of sequence in fragments shorter than     814 bp
  40% of sequence in fragments shorter than     915 bp
  45% of sequence in fragments shorter than    1022 bp
  50% of sequence in fragments shorter than    1139 bp
  55% of sequence in fragments shorter than    1267 bp
  60% of sequence in fragments shorter than    1404 bp
  65% of sequence in fragments shorter than    1561 bp
  70% of sequence in fragments shorter than    1739 bp
  75% of sequence in fragments shorter than    1955 bp
  80% of sequence in fragments shorter than    2222 bp
  85% of sequence in fragments shorter than    2579 bp
  90% of sequence in fragments shorter than    3089 bp
  95% of sequence in fragments shorter than    4046 bp

# AccI.SfeI (GTMKAC+CTRYAG) double digest, 4631 thousand fragments (1101063+3531009 sites), 643 bp average fragment length
   5% of sequence in fragments shorter than     231 bp
  10% of sequence in fragments shorter than     346 bp
  15% of sequence in fragments shorter than     446 bp
  20% of sequence in fragments shorter than     536 bp
  25% of sequence in fragments shorter than     629 bp
  30% of sequence in fragments shorter than     716 bp
  35% of sequence in fragments shorter than     811 bp
  40% of sequence in fragments shorter than     903 bp
  45% of sequence in fragments shorter than    1003 bp
  50% of sequence in fragments shorter than    1104 bp
  55% of sequence in fragments shorter than    1216 bp
  60% of sequence in fragments shorter than    1338 bp
  65% of sequence in fragments shorter than    1465 bp
  70% of sequence in fragments shorter than    1616 bp
  75% of sequence in fragments shorter than    1790 bp
  80% of sequence in fragments shorter than    1995 bp
  85% of sequence in fragments shorter than    2236 bp
  90% of sequence in fragments shorter than    2599 bp
  95% of sequence in fragments shorter than    3227 bp

# BscAI.BspHI (GCATC+TCATGA) double digest, 4630 thousand fragments (3652926+978290 sites), 643 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     338 bp
  15% of sequence in fragments shorter than     439 bp
  20% of sequence in fragments shorter than     538 bp
  25% of sequence in fragments shorter than     635 bp
  30% of sequence in fragments shorter than     730 bp
  35% of sequence in fragments shorter than     828 bp
  40% of sequence in fragments shorter than     930 bp
  45% of sequence in fragments shorter than    1037 bp
  50% of sequence in fragments shorter than    1151 bp
  55% of sequence in fragments shorter than    1269 bp
  60% of sequence in fragments shorter than    1398 bp
  65% of sequence in fragments shorter than    1545 bp
  70% of sequence in fragments shorter than    1702 bp
  75% of sequence in fragments shorter than    1892 bp
  80% of sequence in fragments shorter than    2115 bp
  85% of sequence in fragments shorter than    2407 bp
  90% of sequence in fragments shorter than    2819 bp
  95% of sequence in fragments shorter than    3567 bp

# BciVI.MlyI (GTATCC+GAGTC) double digest, 4627 thousand fragments (696093+3931692 sites), 644 bp average fragment length
   5% of sequence in fragments shorter than     225 bp
  10% of sequence in fragments shorter than     336 bp
  15% of sequence in fragments shorter than     433 bp
  20% of sequence in fragments shorter than     526 bp
  25% of sequence in fragments shorter than     617 bp
  30% of sequence in fragments shorter than     707 bp
  35% of sequence in fragments shorter than     797 bp
  40% of sequence in fragments shorter than     891 bp
  45% of sequence in fragments shorter than     991 bp
  50% of sequence in fragments shorter than    1098 bp
  55% of sequence in fragments shorter than    1214 bp
  60% of sequence in fragments shorter than    1340 bp
  65% of sequence in fragments shorter than    1481 bp
  70% of sequence in fragments shorter than    1644 bp
  75% of sequence in fragments shorter than    1820 bp
  80% of sequence in fragments shorter than    2041 bp
  85% of sequence in fragments shorter than    2327 bp
  90% of sequence in fragments shorter than    2744 bp
  95% of sequence in fragments shorter than    3505 bp

# SacI.Tsp45I (GAGCTC+GTSAC) double digest, 4624 thousand fragments (603323+4021761 sites), 644 bp average fragment length
   5% of sequence in fragments shorter than     221 bp
  10% of sequence in fragments shorter than     338 bp
  15% of sequence in fragments shorter than     433 bp
  20% of sequence in fragments shorter than     529 bp
  25% of sequence in fragments shorter than     623 bp
  30% of sequence in fragments shorter than     716 bp
  35% of sequence in fragments shorter than     814 bp
  40% of sequence in fragments shorter than     913 bp
  45% of sequence in fragments shorter than    1019 bp
  50% of sequence in fragments shorter than    1133 bp
  55% of sequence in fragments shorter than    1257 bp
  60% of sequence in fragments shorter than    1393 bp
  65% of sequence in fragments shorter than    1549 bp
  70% of sequence in fragments shorter than    1722 bp
  75% of sequence in fragments shorter than    1930 bp
  80% of sequence in fragments shorter than    2187 bp
  85% of sequence in fragments shorter than    2525 bp
  90% of sequence in fragments shorter than    3025 bp
  95% of sequence in fragments shorter than    3955 bp

# AvrII.Tsp45I (CCTAGG+GTSAC) double digest, 4616 thousand fragments (594956+4021761 sites), 645 bp average fragment length
   5% of sequence in fragments shorter than     226 bp
  10% of sequence in fragments shorter than     342 bp
  15% of sequence in fragments shorter than     437 bp
  20% of sequence in fragments shorter than     535 bp
  25% of sequence in fragments shorter than     628 bp
  30% of sequence in fragments shorter than     716 bp
  35% of sequence in fragments shorter than     813 bp
  40% of sequence in fragments shorter than     911 bp
  45% of sequence in fragments shorter than    1017 bp
  50% of sequence in fragments shorter than    1128 bp
  55% of sequence in fragments shorter than    1246 bp
  60% of sequence in fragments shorter than    1375 bp
  65% of sequence in fragments shorter than    1524 bp
  70% of sequence in fragments shorter than    1687 bp
  75% of sequence in fragments shorter than    1877 bp
  80% of sequence in fragments shorter than    2099 bp
  85% of sequence in fragments shorter than    2395 bp
  90% of sequence in fragments shorter than    2799 bp
  95% of sequence in fragments shorter than    3538 bp

# PpuMI.SfeI (RGGWCCY+CTRYAG) double digest, 4615 thousand fragments (1085138+3531009 sites), 645 bp average fragment length
   5% of sequence in fragments shorter than     223 bp
  10% of sequence in fragments shorter than     336 bp
  15% of sequence in fragments shorter than     439 bp
  20% of sequence in fragments shorter than     534 bp
  25% of sequence in fragments shorter than     633 bp
  30% of sequence in fragments shorter than     726 bp
  35% of sequence in fragments shorter than     825 bp
  40% of sequence in fragments shorter than     928 bp
  45% of sequence in fragments shorter than    1036 bp
  50% of sequence in fragments shorter than    1147 bp
  55% of sequence in fragments shorter than    1270 bp
  60% of sequence in fragments shorter than    1400 bp
  65% of sequence in fragments shorter than    1551 bp
  70% of sequence in fragments shorter than    1721 bp
  75% of sequence in fragments shorter than    1899 bp
  80% of sequence in fragments shorter than    2129 bp
  85% of sequence in fragments shorter than    2428 bp
  90% of sequence in fragments shorter than    2851 bp
  95% of sequence in fragments shorter than    3592 bp

# PvuII.SfeI (CAGCTG+CTRYAG) double digest, 4614 thousand fragments (1084260+3531009 sites), 645 bp average fragment length
   5% of sequence in fragments shorter than     227 bp
  10% of sequence in fragments shorter than     346 bp
  15% of sequence in fragments shorter than     450 bp
  20% of sequence in fragments shorter than     545 bp
  25% of sequence in fragments shorter than     642 bp
  30% of sequence in fragments shorter than     736 bp
  35% of sequence in fragments shorter than     837 bp
  40% of sequence in fragments shorter than     938 bp
  45% of sequence in fragments shorter than    1043 bp
  50% of sequence in fragments shorter than    1157 bp
  55% of sequence in fragments shorter than    1278 bp
  60% of sequence in fragments shorter than    1406 bp
  65% of sequence in fragments shorter than    1555 bp
  70% of sequence in fragments shorter than    1722 bp
  75% of sequence in fragments shorter than    1916 bp
  80% of sequence in fragments shorter than    2132 bp
  85% of sequence in fragments shorter than    2423 bp
  90% of sequence in fragments shorter than    2839 bp
  95% of sequence in fragments shorter than    3567 bp

# AciI.SpeI (CCGC+ACTAGT) double digest, 4605 thousand fragments (4206130+400286 sites), 646 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     385 bp
  15% of sequence in fragments shorter than     551 bp
  20% of sequence in fragments shorter than     712 bp
  25% of sequence in fragments shorter than     868 bp
  30% of sequence in fragments shorter than    1033 bp
  35% of sequence in fragments shorter than    1205 bp
  40% of sequence in fragments shorter than    1386 bp
  45% of sequence in fragments shorter than    1579 bp
  50% of sequence in fragments shorter than    1793 bp
  55% of sequence in fragments shorter than    2025 bp
  60% of sequence in fragments shorter than    2282 bp
  65% of sequence in fragments shorter than    2571 bp
  70% of sequence in fragments shorter than    2899 bp
  75% of sequence in fragments shorter than    3292 bp
  80% of sequence in fragments shorter than    3773 bp
  85% of sequence in fragments shorter than    4390 bp
  90% of sequence in fragments shorter than    5239 bp
  95% of sequence in fragments shorter than    6745 bp

# AflIII.StyI (ACRYGT+CCWWGG) double digest, 4599 thousand fragments (1485394+3114107 sites), 647 bp average fragment length
   5% of sequence in fragments shorter than     222 bp
  10% of sequence in fragments shorter than     340 bp
  15% of sequence in fragments shorter than     444 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     639 bp
  30% of sequence in fragments shorter than     732 bp
  35% of sequence in fragments shorter than     829 bp
  40% of sequence in fragments shorter than     932 bp
  45% of sequence in fragments shorter than    1040 bp
  50% of sequence in fragments shorter than    1155 bp
  55% of sequence in fragments shorter than    1280 bp
  60% of sequence in fragments shorter than    1414 bp
  65% of sequence in fragments shorter than    1567 bp
  70% of sequence in fragments shorter than    1741 bp
  75% of sequence in fragments shorter than    1942 bp
  80% of sequence in fragments shorter than    2185 bp
  85% of sequence in fragments shorter than    2506 bp
  90% of sequence in fragments shorter than    2947 bp
  95% of sequence in fragments shorter than    3726 bp

# AciI.HpaI (CCGC+GTTAAC) double digest, 4595 thousand fragments (4206130+389418 sites), 648 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     387 bp
  15% of sequence in fragments shorter than     554 bp
  20% of sequence in fragments shorter than     715 bp
  25% of sequence in fragments shorter than     876 bp
  30% of sequence in fragments shorter than    1039 bp
  35% of sequence in fragments shorter than    1216 bp
  40% of sequence in fragments shorter than    1395 bp
  45% of sequence in fragments shorter than    1592 bp
  50% of sequence in fragments shorter than    1806 bp
  55% of sequence in fragments shorter than    2042 bp
  60% of sequence in fragments shorter than    2299 bp
  65% of sequence in fragments shorter than    2593 bp
  70% of sequence in fragments shorter than    2929 bp
  75% of sequence in fragments shorter than    3326 bp
  80% of sequence in fragments shorter than    3812 bp
  85% of sequence in fragments shorter than    4442 bp
  90% of sequence in fragments shorter than    5335 bp
  95% of sequence in fragments shorter than    6880 bp

# HgaI.Tsp45I (GACGC+GTSAC) double digest, 4593 thousand fragments (571889+4021761 sites), 648 bp average fragment length
   5% of sequence in fragments shorter than     224 bp
  10% of sequence in fragments shorter than     342 bp
  15% of sequence in fragments shorter than     440 bp
  20% of sequence in fragments shorter than     540 bp
  25% of sequence in fragments shorter than     635 bp
  30% of sequence in fragments shorter than     732 bp
  35% of sequence in fragments shorter than     833 bp
  40% of sequence in fragments shorter than     936 bp
  45% of sequence in fragments shorter than    1046 bp
  50% of sequence in fragments shorter than    1166 bp
  55% of sequence in fragments shorter than    1293 bp
  60% of sequence in fragments shorter than    1433 bp
  65% of sequence in fragments shorter than    1592 bp
  70% of sequence in fragments shorter than    1773 bp
  75% of sequence in fragments shorter than    1988 bp
  80% of sequence in fragments shorter than    2251 bp
  85% of sequence in fragments shorter than    2600 bp
  90% of sequence in fragments shorter than    3102 bp
  95% of sequence in fragments shorter than    4013 bp

# EciI.Tsp45I (GGCGGA+GTSAC) double digest, 4592 thousand fragments (571273+4021761 sites), 648 bp average fragment length
   5% of sequence in fragments shorter than     217 bp
  10% of sequence in fragments shorter than     337 bp
  15% of sequence in fragments shorter than     433 bp
  20% of sequence in fragments shorter than     531 bp
  25% of sequence in fragments shorter than     625 bp
  30% of sequence in fragments shorter than     718 bp
  35% of sequence in fragments shorter than     818 bp
  40% of sequence in fragments shorter than     918 bp
  45% of sequence in fragments shorter than    1027 bp
  50% of sequence in fragments shorter than    1143 bp
  55% of sequence in fragments shorter than    1268 bp
  60% of sequence in fragments shorter than    1406 bp
  65% of sequence in fragments shorter than    1566 bp
  70% of sequence in fragments shorter than    1744 bp
  75% of sequence in fragments shorter than    1957 bp
  80% of sequence in fragments shorter than    2224 bp
  85% of sequence in fragments shorter than    2579 bp
  90% of sequence in fragments shorter than    3095 bp
  95% of sequence in fragments shorter than    4060 bp

# AvaIII.BscAI (ATGCAT+GCATC) double digest, 4585 thousand fragments (933357+3652926 sites), 649 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     364 bp
  15% of sequence in fragments shorter than     478 bp
  20% of sequence in fragments shorter than     583 bp
  25% of sequence in fragments shorter than     682 bp
  30% of sequence in fragments shorter than     783 bp
  35% of sequence in fragments shorter than     886 bp
  40% of sequence in fragments shorter than     995 bp
  45% of sequence in fragments shorter than    1110 bp
  50% of sequence in fragments shorter than    1227 bp
  55% of sequence in fragments shorter than    1356 bp
  60% of sequence in fragments shorter than    1493 bp
  65% of sequence in fragments shorter than    1641 bp
  70% of sequence in fragments shorter than    1816 bp
  75% of sequence in fragments shorter than    2017 bp
  80% of sequence in fragments shorter than    2257 bp
  85% of sequence in fragments shorter than    2575 bp
  90% of sequence in fragments shorter than    3023 bp
  95% of sequence in fragments shorter than    3846 bp

# MfeI.Tsp45I (CAATTG+GTSAC) double digest, 4585 thousand fragments (564303+4021761 sites), 649 bp average fragment length
   5% of sequence in fragments shorter than     229 bp
  10% of sequence in fragments shorter than     343 bp
  15% of sequence in fragments shorter than     440 bp
  20% of sequence in fragments shorter than     536 bp
  25% of sequence in fragments shorter than     629 bp
  30% of sequence in fragments shorter than     720 bp
  35% of sequence in fragments shorter than     816 bp
  40% of sequence in fragments shorter than     911 bp
  45% of sequence in fragments shorter than    1014 bp
  50% of sequence in fragments shorter than    1122 bp
  55% of sequence in fragments shorter than    1237 bp
  60% of sequence in fragments shorter than    1367 bp
  65% of sequence in fragments shorter than    1510 bp
  70% of sequence in fragments shorter than    1672 bp
  75% of sequence in fragments shorter than    1859 bp
  80% of sequence in fragments shorter than    2073 bp
  85% of sequence in fragments shorter than    2357 bp
  90% of sequence in fragments shorter than    2745 bp
  95% of sequence in fragments shorter than    3438 bp

# AciI.SapI (CCGC+GCTCTTC) double digest, 4582 thousand fragments (4206130+377161 sites), 650 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     387 bp
  15% of sequence in fragments shorter than     557 bp
  20% of sequence in fragments shorter than     724 bp
  25% of sequence in fragments shorter than     884 bp
  30% of sequence in fragments shorter than    1058 bp
  35% of sequence in fragments shorter than    1241 bp
  40% of sequence in fragments shorter than    1432 bp
  45% of sequence in fragments shorter than    1639 bp
  50% of sequence in fragments shorter than    1872 bp
  55% of sequence in fragments shorter than    2125 bp
  60% of sequence in fragments shorter than    2409 bp
  65% of sequence in fragments shorter than    2729 bp
  70% of sequence in fragments shorter than    3098 bp
  75% of sequence in fragments shorter than    3529 bp
  80% of sequence in fragments shorter than    4068 bp
  85% of sequence in fragments shorter than    4759 bp
  90% of sequence in fragments shorter than    5748 bp
  95% of sequence in fragments shorter than    7462 bp

# AciI.SmaI (CCGC+CCCGGG) double digest, 4582 thousand fragments (4206130+376940 sites), 650 bp average fragment length
   5% of sequence in fragments shorter than     200 bp
  10% of sequence in fragments shorter than     394 bp
  15% of sequence in fragments shorter than     580 bp
  20% of sequence in fragments shorter than     767 bp
  25% of sequence in fragments shorter than     953 bp
  30% of sequence in fragments shorter than    1149 bp
  35% of sequence in fragments shorter than    1361 bp
  40% of sequence in fragments shorter than    1580 bp
  45% of sequence in fragments shorter than    1831 bp
  50% of sequence in fragments shorter than    2101 bp
  55% of sequence in fragments shorter than    2405 bp
  60% of sequence in fragments shorter than    2740 bp
  65% of sequence in fragments shorter than    3123 bp
  70% of sequence in fragments shorter than    3574 bp
  75% of sequence in fragments shorter than    4105 bp
  80% of sequence in fragments shorter than    4760 bp
  85% of sequence in fragments shorter than    5606 bp
  90% of sequence in fragments shorter than    6809 bp
  95% of sequence in fragments shorter than    8886 bp

# BscAI.StuI (GCATC+AGGCCT) double digest, 4578 thousand fragments (3652926+925729 sites), 650 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     343 bp
  15% of sequence in fragments shorter than     445 bp
  20% of sequence in fragments shorter than     546 bp
  25% of sequence in fragments shorter than     642 bp
  30% of sequence in fragments shorter than     733 bp
  35% of sequence in fragments shorter than     835 bp
  40% of sequence in fragments shorter than     940 bp
  45% of sequence in fragments shorter than    1053 bp
  50% of sequence in fragments shorter than    1175 bp
  55% of sequence in fragments shorter than    1301 bp
  60% of sequence in fragments shorter than    1440 bp
  65% of sequence in fragments shorter than    1591 bp
  70% of sequence in fragments shorter than    1760 bp
  75% of sequence in fragments shorter than    1962 bp
  80% of sequence in fragments shorter than    2206 bp
  85% of sequence in fragments shorter than    2514 bp
  90% of sequence in fragments shorter than    2939 bp
  95% of sequence in fragments shorter than    3654 bp

# AflII.MlyI (CTTAAG+GAGTC) double digest, 4574 thousand fragments (643284+3931692 sites), 651 bp average fragment length
   5% of sequence in fragments shorter than     229 bp
  10% of sequence in fragments shorter than     339 bp
  15% of sequence in fragments shorter than     438 bp
  20% of sequence in fragments shorter than     532 bp
  25% of sequence in fragments shorter than     624 bp
  30% of sequence in fragments shorter than     715 bp
  35% of sequence in fragments shorter than     807 bp
  40% of sequence in fragments shorter than     902 bp
  45% of sequence in fragments shorter than    1006 bp
  50% of sequence in fragments shorter than    1113 bp
  55% of sequence in fragments shorter than    1233 bp
  60% of sequence in fragments shorter than    1363 bp
  65% of sequence in fragments shorter than    1508 bp
  70% of sequence in fragments shorter than    1675 bp
  75% of sequence in fragments shorter than    1860 bp
  80% of sequence in fragments shorter than    2092 bp
  85% of sequence in fragments shorter than    2397 bp
  90% of sequence in fragments shorter than    2840 bp
  95% of sequence in fragments shorter than    3689 bp

# SphI.Tsp45I (GCATGC+GTSAC) double digest, 4572 thousand fragments (550952+4021761 sites), 651 bp average fragment length
   5% of sequence in fragments shorter than     225 bp
  10% of sequence in fragments shorter than     342 bp
  15% of sequence in fragments shorter than     437 bp
  20% of sequence in fragments shorter than     534 bp
  25% of sequence in fragments shorter than     628 bp
  30% of sequence in fragments shorter than     721 bp
  35% of sequence in fragments shorter than     819 bp
  40% of sequence in fragments shorter than     917 bp
  45% of sequence in fragments shorter than    1025 bp
  50% of sequence in fragments shorter than    1138 bp
  55% of sequence in fragments shorter than    1261 bp
  60% of sequence in fragments shorter than    1395 bp
  65% of sequence in fragments shorter than    1551 bp
  70% of sequence in fragments shorter than    1724 bp
  75% of sequence in fragments shorter than    1930 bp
  80% of sequence in fragments shorter than    2185 bp
  85% of sequence in fragments shorter than    2520 bp
  90% of sequence in fragments shorter than    3018 bp
  95% of sequence in fragments shorter than    3949 bp

# AciI.BamHI (CCGC+GGATCC) double digest, 4571 thousand fragments (4206130+365999 sites), 651 bp average fragment length
   5% of sequence in fragments shorter than     205 bp
  10% of sequence in fragments shorter than     387 bp
  15% of sequence in fragments shorter than     560 bp
  20% of sequence in fragments shorter than     729 bp
  25% of sequence in fragments shorter than     895 bp
  30% of sequence in fragments shorter than    1069 bp
  35% of sequence in fragments shorter than    1259 bp
  40% of sequence in fragments shorter than    1456 bp
  45% of sequence in fragments shorter than    1674 bp
  50% of sequence in fragments shorter than    1909 bp
  55% of sequence in fragments shorter than    2170 bp
  60% of sequence in fragments shorter than    2460 bp
  65% of sequence in fragments shorter than    2794 bp
  70% of sequence in fragments shorter than    3183 bp
  75% of sequence in fragments shorter than    3643 bp
  80% of sequence in fragments shorter than    4203 bp
  85% of sequence in fragments shorter than    4925 bp
  90% of sequence in fragments shorter than    5963 bp
  95% of sequence in fragments shorter than    7746 bp

# ScaI.Tsp45I (AGTACT+GTSAC) double digest, 4565 thousand fragments (543793+4021761 sites), 652 bp average fragment length
   5% of sequence in fragments shorter than     228 bp
  10% of sequence in fragments shorter than     343 bp
  15% of sequence in fragments shorter than     439 bp
  20% of sequence in fragments shorter than     536 bp
  25% of sequence in fragments shorter than     628 bp
  30% of sequence in fragments shorter than     720 bp
  35% of sequence in fragments shorter than     817 bp
  40% of sequence in fragments shorter than     913 bp
  45% of sequence in fragments shorter than    1018 bp
  50% of sequence in fragments shorter than    1129 bp
  55% of sequence in fragments shorter than    1248 bp
  60% of sequence in fragments shorter than    1378 bp
  65% of sequence in fragments shorter than    1527 bp
  70% of sequence in fragments shorter than    1695 bp
  75% of sequence in fragments shorter than    1891 bp
  80% of sequence in fragments shorter than    2132 bp
  85% of sequence in fragments shorter than    2449 bp
  90% of sequence in fragments shorter than    2916 bp
  95% of sequence in fragments shorter than    3800 bp

# HaeII.MlyI (RGCGCY+GAGTC) double digest, 4561 thousand fragments (630101+3931692 sites), 653 bp average fragment length
   5% of sequence in fragments shorter than     221 bp
  10% of sequence in fragments shorter than     338 bp
  15% of sequence in fragments shorter than     438 bp
  20% of sequence in fragments shorter than     538 bp
  25% of sequence in fragments shorter than     637 bp
  30% of sequence in fragments shorter than     729 bp
  35% of sequence in fragments shorter than     832 bp
  40% of sequence in fragments shorter than     935 bp
  45% of sequence in fragments shorter than    1043 bp
  50% of sequence in fragments shorter than    1164 bp
  55% of sequence in fragments shorter than    1296 bp
  60% of sequence in fragments shorter than    1437 bp
  65% of sequence in fragments shorter than    1598 bp
  70% of sequence in fragments shorter than    1781 bp
  75% of sequence in fragments shorter than    1998 bp
  80% of sequence in fragments shorter than    2256 bp
  85% of sequence in fragments shorter than    2604 bp
  90% of sequence in fragments shorter than    3092 bp
  95% of sequence in fragments shorter than    3921 bp

# BscAI.NdeI (GCATC+CATATG) double digest, 4555 thousand fragments (3652926+903360 sites), 654 bp average fragment length
   5% of sequence in fragments shorter than     223 bp
  10% of sequence in fragments shorter than     344 bp
  15% of sequence in fragments shorter than     452 bp
  20% of sequence in fragments shorter than     553 bp
  25% of sequence in fragments shorter than     647 bp
  30% of sequence in fragments shorter than     743 bp
  35% of sequence in fragments shorter than     842 bp
  40% of sequence in fragments shorter than     945 bp
  45% of sequence in fragments shorter than    1052 bp
  50% of sequence in fragments shorter than    1165 bp
  55% of sequence in fragments shorter than    1280 bp
  60% of sequence in fragments shorter than    1409 bp
  65% of sequence in fragments shorter than    1555 bp
  70% of sequence in fragments shorter than    1717 bp
  75% of sequence in fragments shorter than    1911 bp
  80% of sequence in fragments shorter than    2145 bp
  85% of sequence in fragments shorter than    2447 bp
  90% of sequence in fragments shorter than    2879 bp
  95% of sequence in fragments shorter than    3672 bp

# FauI.StyI (CCCGC+CCWWGG) double digest, 4553 thousand fragments (1439772+3114107 sites), 654 bp average fragment length
   5% of sequence in fragments shorter than     213 bp
  10% of sequence in fragments shorter than     345 bp
  15% of sequence in fragments shorter than     462 bp
  20% of sequence in fragments shorter than     576 bp
  25% of sequence in fragments shorter than     690 bp
  30% of sequence in fragments shorter than     799 bp
  35% of sequence in fragments shorter than     921 bp
  40% of sequence in fragments shorter than    1049 bp
  45% of sequence in fragments shorter than    1187 bp
  50% of sequence in fragments shorter than    1334 bp
  55% of sequence in fragments shorter than    1488 bp
  60% of sequence in fragments shorter than    1667 bp
  65% of sequence in fragments shorter than    1869 bp
  70% of sequence in fragments shorter than    2098 bp
  75% of sequence in fragments shorter than    2374 bp
  80% of sequence in fragments shorter than    2712 bp
  85% of sequence in fragments shorter than    3136 bp
  90% of sequence in fragments shorter than    3724 bp
  95% of sequence in fragments shorter than    4863 bp

# BspMI.StyI (ACCTGC+CCWWGG) double digest, 4551 thousand fragments (1438129+3114107 sites), 654 bp average fragment length
   5% of sequence in fragments shorter than     221 bp
  10% of sequence in fragments shorter than     339 bp
  15% of sequence in fragments shorter than     442 bp
  20% of sequence in fragments shorter than     544 bp
  25% of sequence in fragments shorter than     646 bp
  30% of sequence in fragments shorter than     748 bp
  35% of sequence in fragments shorter than     850 bp
  40% of sequence in fragments shorter than     959 bp
  45% of sequence in fragments shorter than    1076 bp
  50% of sequence in fragments shorter than    1204 bp
  55% of sequence in fragments shorter than    1339 bp
  60% of sequence in fragments shorter than    1488 bp
  65% of sequence in fragments shorter than    1641 bp
  70% of sequence in fragments shorter than    1833 bp
  75% of sequence in fragments shorter than    2057 bp
  80% of sequence in fragments shorter than    2333 bp
  85% of sequence in fragments shorter than    2690 bp
  90% of sequence in fragments shorter than    3182 bp
  95% of sequence in fragments shorter than    4071 bp

# FauI.NspI (CCCGC+RCATGY) double digest, 4543 thousand fragments (1439772+3104359 sites), 655 bp average fragment length
   5% of sequence in fragments shorter than     225 bp
  10% of sequence in fragments shorter than     354 bp
  15% of sequence in fragments shorter than     467 bp
  20% of sequence in fragments shorter than     577 bp
  25% of sequence in fragments shorter than     684 bp
  30% of sequence in fragments shorter than     789 bp
  35% of sequence in fragments shorter than     898 bp
  40% of sequence in fragments shorter than    1010 bp
  45% of sequence in fragments shorter than    1124 bp
  50% of sequence in fragments shorter than    1247 bp
  55% of sequence in fragments shorter than    1381 bp
  60% of sequence in fragments shorter than    1528 bp
  65% of sequence in fragments shorter than    1691 bp
  70% of sequence in fragments shorter than    1877 bp
  75% of sequence in fragments shorter than    2095 bp
  80% of sequence in fragments shorter than    2362 bp
  85% of sequence in fragments shorter than    2702 bp
  90% of sequence in fragments shorter than    3190 bp
  95% of sequence in fragments shorter than    4059 bp

# BspMI.NspI (ACCTGC+RCATGY) double digest, 4542 thousand fragments (1438129+3104359 sites), 656 bp average fragment length
   5% of sequence in fragments shorter than     229 bp
  10% of sequence in fragments shorter than     348 bp
  15% of sequence in fragments shorter than     455 bp
  20% of sequence in fragments shorter than     556 bp
  25% of sequence in fragments shorter than     656 bp
  30% of sequence in fragments shorter than     752 bp
  35% of sequence in fragments shorter than     852 bp
  40% of sequence in fragments shorter than     952 bp
  45% of sequence in fragments shorter than    1060 bp
  50% of sequence in fragments shorter than    1173 bp
  55% of sequence in fragments shorter than    1296 bp
  60% of sequence in fragments shorter than    1426 bp
  65% of sequence in fragments shorter than    1575 bp
  70% of sequence in fragments shorter than    1743 bp
  75% of sequence in fragments shorter than    1934 bp
  80% of sequence in fragments shorter than    2162 bp
  85% of sequence in fragments shorter than    2459 bp
  90% of sequence in fragments shorter than    2851 bp
  95% of sequence in fragments shorter than    3531 bp

# AciI.BstEII (CCGC+GGTNACC) double digest, 4541 thousand fragments (4206130+335865 sites), 656 bp average fragment length
   5% of sequence in fragments shorter than     206 bp
  10% of sequence in fragments shorter than     391 bp
  15% of sequence in fragments shorter than     565 bp
  20% of sequence in fragments shorter than     737 bp
  25% of sequence in fragments shorter than     904 bp
  30% of sequence in fragments shorter than    1081 bp
  35% of sequence in fragments shorter than    1269 bp
  40% of sequence in fragments shorter than    1466 bp
  45% of sequence in fragments shorter than    1686 bp
  50% of sequence in fragments shorter than    1925 bp
  55% of sequence in fragments shorter than    2192 bp
  60% of sequence in fragments shorter than    2489 bp
  65% of sequence in fragments shorter than    2821 bp
  70% of sequence in fragments shorter than    3204 bp
  75% of sequence in fragments shorter than    3664 bp
  80% of sequence in fragments shorter than    4222 bp
  85% of sequence in fragments shorter than    4941 bp
  90% of sequence in fragments shorter than    5962 bp
  95% of sequence in fragments shorter than    7744 bp

# AvaI.SfeI (CYCGRG+CTRYAG) double digest, 4536 thousand fragments (1005714+3531009 sites), 656 bp average fragment length
   5% of sequence in fragments shorter than     233 bp
  10% of sequence in fragments shorter than     353 bp
  15% of sequence in fragments shorter than     460 bp
  20% of sequence in fragments shorter than     560 bp
  25% of sequence in fragments shorter than     660 bp
  30% of sequence in fragments shorter than     761 bp
  35% of sequence in fragments shorter than     867 bp
  40% of sequence in fragments shorter than     977 bp
  45% of sequence in fragments shorter than    1086 bp
  50% of sequence in fragments shorter than    1209 bp
  55% of sequence in fragments shorter than    1342 bp
  60% of sequence in fragments shorter than    1481 bp
  65% of sequence in fragments shorter than    1642 bp
  70% of sequence in fragments shorter than    1823 bp
  75% of sequence in fragments shorter than    2033 bp
  80% of sequence in fragments shorter than    2261 bp
  85% of sequence in fragments shorter than    2587 bp
  90% of sequence in fragments shorter than    3043 bp
  95% of sequence in fragments shorter than    3850 bp

# MlyI.SacI (GAGTC+GAGCTC) double digest, 4534 thousand fragments (3931692+603323 sites), 657 bp average fragment length
   5% of sequence in fragments shorter than     225 bp
  10% of sequence in fragments shorter than     337 bp
  15% of sequence in fragments shorter than     439 bp
  20% of sequence in fragments shorter than     535 bp
  25% of sequence in fragments shorter than     630 bp
  30% of sequence in fragments shorter than     725 bp
  35% of sequence in fragments shorter than     823 bp
  40% of sequence in fragments shorter than     923 bp
  45% of sequence in fragments shorter than    1030 bp
  50% of sequence in fragments shorter than    1145 bp
  55% of sequence in fragments shorter than    1271 bp
  60% of sequence in fragments shorter than    1408 bp
  65% of sequence in fragments shorter than    1563 bp
  70% of sequence in fragments shorter than    1740 bp
  75% of sequence in fragments shorter than    1953 bp
  80% of sequence in fragments shorter than    2215 bp
  85% of sequence in fragments shorter than    2570 bp
  90% of sequence in fragments shorter than    3076 bp
  95% of sequence in fragments shorter than    4023 bp

# AvrII.MlyI (CCTAGG+GAGTC) double digest, 4526 thousand fragments (594956+3931692 sites), 658 bp average fragment length
   5% of sequence in fragments shorter than     230 bp
  10% of sequence in fragments shorter than     343 bp
  15% of sequence in fragments shorter than     445 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     636 bp
  30% of sequence in fragments shorter than     727 bp
  35% of sequence in fragments shorter than     823 bp
  40% of sequence in fragments shorter than     921 bp
  45% of sequence in fragments shorter than    1026 bp
  50% of sequence in fragments shorter than    1140 bp
  55% of sequence in fragments shorter than    1264 bp
  60% of sequence in fragments shorter than    1397 bp
  65% of sequence in fragments shorter than    1547 bp
  70% of sequence in fragments shorter than    1716 bp
  75% of sequence in fragments shorter than    1909 bp
  80% of sequence in fragments shorter than    2147 bp
  85% of sequence in fragments shorter than    2468 bp
  90% of sequence in fragments shorter than    2928 bp
  95% of sequence in fragments shorter than    3818 bp

# ApaLI.Tsp45I (GTGCAC+GTSAC) double digest, 4517 thousand fragments (496255+4021761 sites), 659 bp average fragment length
   5% of sequence in fragments shorter than     228 bp
  10% of sequence in fragments shorter than     344 bp
  15% of sequence in fragments shorter than     444 bp
  20% of sequence in fragments shorter than     543 bp
  25% of sequence in fragments shorter than     638 bp
  30% of sequence in fragments shorter than     732 bp
  35% of sequence in fragments shorter than     832 bp
  40% of sequence in fragments shorter than     933 bp
  45% of sequence in fragments shorter than    1040 bp
  50% of sequence in fragments shorter than    1156 bp
  55% of sequence in fragments shorter than    1279 bp
  60% of sequence in fragments shorter than    1416 bp
  65% of sequence in fragments shorter than    1571 bp
  70% of sequence in fragments shorter than    1745 bp
  75% of sequence in fragments shorter than    1951 bp
  80% of sequence in fragments shorter than    2206 bp
  85% of sequence in fragments shorter than    2539 bp
  90% of sequence in fragments shorter than    3025 bp
  95% of sequence in fragments shorter than    3835 bp

# BscAI.HindIII (GCATC+AAGCTT) double digest, 4512 thousand fragments (3652926+860365 sites), 660 bp average fragment length
   5% of sequence in fragments shorter than     224 bp
  10% of sequence in fragments shorter than     347 bp
  15% of sequence in fragments shorter than     455 bp
  20% of sequence in fragments shorter than     556 bp
  25% of sequence in fragments shorter than     651 bp
  30% of sequence in fragments shorter than     749 bp
  35% of sequence in fragments shorter than     849 bp
  40% of sequence in fragments shorter than     954 bp
  45% of sequence in fragments shorter than    1063 bp
  50% of sequence in fragments shorter than    1179 bp
  55% of sequence in fragments shorter than    1302 bp
  60% of sequence in fragments shorter than    1433 bp
  65% of sequence in fragments shorter than    1581 bp
  70% of sequence in fragments shorter than    1740 bp
  75% of sequence in fragments shorter than    1935 bp
  80% of sequence in fragments shorter than    2169 bp
  85% of sequence in fragments shorter than    2470 bp
  90% of sequence in fragments shorter than    2897 bp
  95% of sequence in fragments shorter than    3656 bp

# BspHI.SfeI (TCATGA+CTRYAG) double digest, 4508 thousand fragments (978290+3531009 sites), 660 bp average fragment length
   5% of sequence in fragments shorter than     230 bp
  10% of sequence in fragments shorter than     347 bp
  15% of sequence in fragments shorter than     448 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     637 bp
  30% of sequence in fragments shorter than     727 bp
  35% of sequence in fragments shorter than     823 bp
  40% of sequence in fragments shorter than     923 bp
  45% of sequence in fragments shorter than    1027 bp
  50% of sequence in fragments shorter than    1131 bp
  55% of sequence in fragments shorter than    1248 bp
  60% of sequence in fragments shorter than    1375 bp
  65% of sequence in fragments shorter than    1509 bp
  70% of sequence in fragments shorter than    1666 bp
  75% of sequence in fragments shorter than    1832 bp
  80% of sequence in fragments shorter than    2042 bp
  85% of sequence in fragments shorter than    2312 bp
  90% of sequence in fragments shorter than    2692 bp
  95% of sequence in fragments shorter than    3342 bp

# AvaII.HhaI (GGWCC+GCGC) double digest, 4503 thousand fragments (2747690+1756502 sites), 661 bp average fragment length
   5% of sequence in fragments shorter than     207 bp
  10% of sequence in fragments shorter than     345 bp
  15% of sequence in fragments shorter than     472 bp
  20% of sequence in fragments shorter than     602 bp
  25% of sequence in fragments shorter than     724 bp
  30% of sequence in fragments shorter than     862 bp
  35% of sequence in fragments shorter than     990 bp
  40% of sequence in fragments shorter than    1138 bp
  45% of sequence in fragments shorter than    1299 bp
  50% of sequence in fragments shorter than    1471 bp
  55% of sequence in fragments shorter than    1654 bp
  60% of sequence in fragments shorter than    1856 bp
  65% of sequence in fragments shorter than    2088 bp
  70% of sequence in fragments shorter than    2352 bp
  75% of sequence in fragments shorter than    2658 bp
  80% of sequence in fragments shorter than    3037 bp
  85% of sequence in fragments shorter than    3526 bp
  90% of sequence in fragments shorter than    4218 bp
  95% of sequence in fragments shorter than    5408 bp

# AciI.SexAI (CCGC+ACCWGGT) double digest, 4503 thousand fragments (4206130+297900 sites), 661 bp average fragment length
   5% of sequence in fragments shorter than     207 bp
  10% of sequence in fragments shorter than     394 bp
  15% of sequence in fragments shorter than     569 bp
  20% of sequence in fragments shorter than     741 bp
  25% of sequence in fragments shorter than     913 bp
  30% of sequence in fragments shorter than    1090 bp
  35% of sequence in fragments shorter than    1283 bp
  40% of sequence in fragments shorter than    1481 bp
  45% of sequence in fragments shorter than    1705 bp
  50% of sequence in fragments shorter than    1944 bp
  55% of sequence in fragments shorter than    2211 bp
  60% of sequence in fragments shorter than    2509 bp
  65% of sequence in fragments shorter than    2846 bp
  70% of sequence in fragments shorter than    3238 bp
  75% of sequence in fragments shorter than    3705 bp
  80% of sequence in fragments shorter than    4273 bp
  85% of sequence in fragments shorter than    5006 bp
  90% of sequence in fragments shorter than    6045 bp
  95% of sequence in fragments shorter than    7841 bp

# BscAI.XbaI (GCATC+TCTAGA) double digest, 4503 thousand fragments (3652926+850998 sites), 661 bp average fragment length
   5% of sequence in fragments shorter than     227 bp
  10% of sequence in fragments shorter than     347 bp
  15% of sequence in fragments shorter than     455 bp
  20% of sequence in fragments shorter than     557 bp
  25% of sequence in fragments shorter than     657 bp
  30% of sequence in fragments shorter than     752 bp
  35% of sequence in fragments shorter than     851 bp
  40% of sequence in fragments shorter than     948 bp
  45% of sequence in fragments shorter than    1057 bp
  50% of sequence in fragments shorter than    1173 bp
  55% of sequence in fragments shorter than    1295 bp
  60% of sequence in fragments shorter than    1421 bp
  65% of sequence in fragments shorter than    1569 bp
  70% of sequence in fragments shorter than    1733 bp
  75% of sequence in fragments shorter than    1929 bp
  80% of sequence in fragments shorter than    2161 bp
  85% of sequence in fragments shorter than    2457 bp
  90% of sequence in fragments shorter than    2871 bp
  95% of sequence in fragments shorter than    3593 bp

# HgaI.MlyI (GACGC+GAGTC) double digest, 4503 thousand fragments (571889+3931692 sites), 661 bp average fragment length
   5% of sequence in fragments shorter than     226 bp
  10% of sequence in fragments shorter than     340 bp
  15% of sequence in fragments shorter than     444 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     640 bp
  30% of sequence in fragments shorter than     739 bp
  35% of sequence in fragments shorter than     839 bp
  40% of sequence in fragments shorter than     943 bp
  45% of sequence in fragments shorter than    1056 bp
  50% of sequence in fragments shorter than    1176 bp
  55% of sequence in fragments shorter than    1308 bp
  60% of sequence in fragments shorter than    1450 bp
  65% of sequence in fragments shorter than    1612 bp
  70% of sequence in fragments shorter than    1795 bp
  75% of sequence in fragments shorter than    2014 bp
  80% of sequence in fragments shorter than    2289 bp
  85% of sequence in fragments shorter than    2658 bp
  90% of sequence in fragments shorter than    3177 bp
  95% of sequence in fragments shorter than    4141 bp

# EciI.MlyI (GGCGGA+GAGTC) double digest, 4502 thousand fragments (571273+3931692 sites), 661 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     337 bp
  15% of sequence in fragments shorter than     445 bp
  20% of sequence in fragments shorter than     548 bp
  25% of sequence in fragments shorter than     649 bp
  30% of sequence in fragments shorter than     750 bp
  35% of sequence in fragments shorter than     855 bp
  40% of sequence in fragments shorter than     962 bp
  45% of sequence in fragments shorter than    1076 bp
  50% of sequence in fragments shorter than    1201 bp
  55% of sequence in fragments shorter than    1336 bp
  60% of sequence in fragments shorter than    1484 bp
  65% of sequence in fragments shorter than    1652 bp
  70% of sequence in fragments shorter than    1841 bp
  75% of sequence in fragments shorter than    2068 bp
  80% of sequence in fragments shorter than    2358 bp
  85% of sequence in fragments shorter than    2744 bp
  90% of sequence in fragments shorter than    3291 bp
  95% of sequence in fragments shorter than    4331 bp

# BscAI.EcoRI (GCATC+GAATTC) double digest, 4499 thousand fragments (3652926+847401 sites), 662 bp average fragment length
   5% of sequence in fragments shorter than     225 bp
  10% of sequence in fragments shorter than     345 bp
  15% of sequence in fragments shorter than     452 bp
  20% of sequence in fragments shorter than     555 bp
  25% of sequence in fragments shorter than     651 bp
  30% of sequence in fragments shorter than     746 bp
  35% of sequence in fragments shorter than     847 bp
  40% of sequence in fragments shorter than     952 bp
  45% of sequence in fragments shorter than    1062 bp
  50% of sequence in fragments shorter than    1178 bp
  55% of sequence in fragments shorter than    1300 bp
  60% of sequence in fragments shorter than    1429 bp
  65% of sequence in fragments shorter than    1572 bp
  70% of sequence in fragments shorter than    1738 bp
  75% of sequence in fragments shorter than    1930 bp
  80% of sequence in fragments shorter than    2164 bp
  85% of sequence in fragments shorter than    2457 bp
  90% of sequence in fragments shorter than    2871 bp
  95% of sequence in fragments shorter than    3588 bp

# MfeI.MlyI (CAATTG+GAGTC) double digest, 4495 thousand fragments (564303+3931692 sites), 662 bp average fragment length
   5% of sequence in fragments shorter than     233 bp
  10% of sequence in fragments shorter than     346 bp
  15% of sequence in fragments shorter than     447 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     636 bp
  30% of sequence in fragments shorter than     728 bp
  35% of sequence in fragments shorter than     822 bp
  40% of sequence in fragments shorter than     917 bp
  45% of sequence in fragments shorter than    1019 bp
  50% of sequence in fragments shorter than    1127 bp
  55% of sequence in fragments shorter than    1247 bp
  60% of sequence in fragments shorter than    1378 bp
  65% of sequence in fragments shorter than    1522 bp
  70% of sequence in fragments shorter than    1686 bp
  75% of sequence in fragments shorter than    1873 bp
  80% of sequence in fragments shorter than    2090 bp
  85% of sequence in fragments shorter than    2386 bp
  90% of sequence in fragments shorter than    2812 bp
  95% of sequence in fragments shorter than    3586 bp

# AciI.KpnI (CCGC+GGTACC) double digest, 4493 thousand fragments (4206130+287974 sites), 663 bp average fragment length
   5% of sequence in fragments shorter than     208 bp
  10% of sequence in fragments shorter than     396 bp
  15% of sequence in fragments shorter than     574 bp
  20% of sequence in fragments shorter than     746 bp
  25% of sequence in fragments shorter than     918 bp
  30% of sequence in fragments shorter than    1093 bp
  35% of sequence in fragments shorter than    1272 bp
  40% of sequence in fragments shorter than    1472 bp
  45% of sequence in fragments shorter than    1691 bp
  50% of sequence in fragments shorter than    1932 bp
  55% of sequence in fragments shorter than    2200 bp
  60% of sequence in fragments shorter than    2495 bp
  65% of sequence in fragments shorter than    2827 bp
  70% of sequence in fragments shorter than    3217 bp
  75% of sequence in fragments shorter than    3676 bp
  80% of sequence in fragments shorter than    4245 bp
  85% of sequence in fragments shorter than    4979 bp
  90% of sequence in fragments shorter than    6022 bp
  95% of sequence in fragments shorter than    7829 bp

# AciI.NheI (CCGC+GCTAGC) double digest, 4493 thousand fragments (4206130+287384 sites), 663 bp average fragment length
   5% of sequence in fragments shorter than     208 bp
  10% of sequence in fragments shorter than     398 bp
  15% of sequence in fragments shorter than     576 bp
  20% of sequence in fragments shorter than     750 bp
  25% of sequence in fragments shorter than     920 bp
  30% of sequence in fragments shorter than    1095 bp
  35% of sequence in fragments shorter than    1289 bp
  40% of sequence in fragments shorter than    1484 bp
  45% of sequence in fragments shorter than    1702 bp
  50% of sequence in fragments shorter than    1929 bp
  55% of sequence in fragments shorter than    2194 bp
  60% of sequence in fragments shorter than    2483 bp
  65% of sequence in fragments shorter than    2814 bp
  70% of sequence in fragments shorter than    3201 bp
  75% of sequence in fragments shorter than    3657 bp
  80% of sequence in fragments shorter than    4214 bp
  85% of sequence in fragments shorter than    4940 bp
  90% of sequence in fragments shorter than    5965 bp
  95% of sequence in fragments shorter than    7728 bp

# HhaI.SmlI (GCGC+CTYRAG) double digest, 4483 thousand fragments (1756502+2727867 sites), 664 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     348 bp
  15% of sequence in fragments shorter than     470 bp
  20% of sequence in fragments shorter than     584 bp
  25% of sequence in fragments shorter than     690 bp
  30% of sequence in fragments shorter than     802 bp
  35% of sequence in fragments shorter than     917 bp
  40% of sequence in fragments shorter than    1035 bp
  45% of sequence in fragments shorter than    1161 bp
  50% of sequence in fragments shorter than    1297 bp
  55% of sequence in fragments shorter than    1441 bp
  60% of sequence in fragments shorter than    1604 bp
  65% of sequence in fragments shorter than    1777 bp
  70% of sequence in fragments shorter than    1979 bp
  75% of sequence in fragments shorter than    2214 bp
  80% of sequence in fragments shorter than    2499 bp
  85% of sequence in fragments shorter than    2838 bp
  90% of sequence in fragments shorter than    3342 bp
  95% of sequence in fragments shorter than    4192 bp

# MlyI.SphI (GAGTC+GCATGC) double digest, 4482 thousand fragments (3931692+550952 sites), 664 bp average fragment length
   5% of sequence in fragments shorter than     229 bp
  10% of sequence in fragments shorter than     343 bp
  15% of sequence in fragments shorter than     445 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     637 bp
  30% of sequence in fragments shorter than     732 bp
  35% of sequence in fragments shorter than     829 bp
  40% of sequence in fragments shorter than     929 bp
  45% of sequence in fragments shorter than    1036 bp
  50% of sequence in fragments shorter than    1151 bp
  55% of sequence in fragments shorter than    1278 bp
  60% of sequence in fragments shorter than    1414 bp
  65% of sequence in fragments shorter than    1570 bp
  70% of sequence in fragments shorter than    1744 bp
  75% of sequence in fragments shorter than    1955 bp
  80% of sequence in fragments shorter than    2218 bp
  85% of sequence in fragments shorter than    2571 bp
  90% of sequence in fragments shorter than    3081 bp
  95% of sequence in fragments shorter than    4041 bp

# ApaI.Tsp45I (GGGCCC+GTSAC) double digest, 4481 thousand fragments (460339+4021761 sites), 664 bp average fragment length
   5% of sequence in fragments shorter than     227 bp
  10% of sequence in fragments shorter than     344 bp
  15% of sequence in fragments shorter than     446 bp
  20% of sequence in fragments shorter than     547 bp
  25% of sequence in fragments shorter than     645 bp
  30% of sequence in fragments shorter than     744 bp
  35% of sequence in fragments shorter than     849 bp
  40% of sequence in fragments shorter than     953 bp
  45% of sequence in fragments shorter than    1066 bp
  50% of sequence in fragments shorter than    1188 bp
  55% of sequence in fragments shorter than    1320 bp
  60% of sequence in fragments shorter than    1466 bp
  65% of sequence in fragments shorter than    1631 bp
  70% of sequence in fragments shorter than    1819 bp
  75% of sequence in fragments shorter than    2044 bp
  80% of sequence in fragments shorter than    2320 bp
  85% of sequence in fragments shorter than    2689 bp
  90% of sequence in fragments shorter than    3231 bp
  95% of sequence in fragments shorter than    4219 bp

# MlyI.ScaI (GAGTC+AGTACT) double digest, 4475 thousand fragments (3931692+543793 sites), 665 bp average fragment length
   5% of sequence in fragments shorter than     233 bp
  10% of sequence in fragments shorter than     345 bp
  15% of sequence in fragments shorter than     446 bp
  20% of sequence in fragments shorter than     542 bp
  25% of sequence in fragments shorter than     636 bp
  30% of sequence in fragments shorter than     729 bp
  35% of sequence in fragments shorter than     825 bp
  40% of sequence in fragments shorter than     923 bp
  45% of sequence in fragments shorter than    1026 bp
  50% of sequence in fragments shorter than    1137 bp
  55% of sequence in fragments shorter than    1259 bp
  60% of sequence in fragments shorter than    1391 bp
  65% of sequence in fragments shorter than    1540 bp
  70% of sequence in fragments shorter than    1708 bp
  75% of sequence in fragments shorter than    1902 bp
  80% of sequence in fragments shorter than    2151 bp
  85% of sequence in fragments shorter than    2481 bp
  90% of sequence in fragments shorter than    2956 bp
  95% of sequence in fragments shorter than    3858 bp

# EcoRV.Tsp45I (GATATC+GTSAC) double digest, 4467 thousand fragments (446473+4021761 sites), 667 bp average fragment length
   5% of sequence in fragments shorter than     235 bp
  10% of sequence in fragments shorter than     348 bp
  15% of sequence in fragments shorter than     451 bp
  20% of sequence in fragments shorter than     549 bp
  25% of sequence in fragments shorter than     643 bp
  30% of sequence in fragments shorter than     734 bp
  35% of sequence in fragments shorter than     831 bp
  40% of sequence in fragments shorter than     932 bp
  45% of sequence in fragments shorter than    1037 bp
  50% of sequence in fragments shorter than    1151 bp
  55% of sequence in fragments shorter than    1272 bp
  60% of sequence in fragments shorter than    1402 bp
  65% of sequence in fragments shorter than    1553 bp
  70% of sequence in fragments shorter than    1720 bp
  75% of sequence in fragments shorter than    1916 bp
  80% of sequence in fragments shorter than    2155 bp
  85% of sequence in fragments shorter than    2461 bp
  90% of sequence in fragments shorter than    2903 bp
  95% of sequence in fragments shorter than    3647 bp

# AvaIII.SfeI (ATGCAT+CTRYAG) double digest, 4463 thousand fragments (933357+3531009 sites), 667 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     353 bp
  15% of sequence in fragments shorter than     455 bp
  20% of sequence in fragments shorter than     547 bp
  25% of sequence in fragments shorter than     642 bp
  30% of sequence in fragments shorter than     733 bp
  35% of sequence in fragments shorter than     831 bp
  40% of sequence in fragments shorter than     929 bp
  45% of sequence in fragments shorter than    1031 bp
  50% of sequence in fragments shorter than    1136 bp
  55% of sequence in fragments shorter than    1253 bp
  60% of sequence in fragments shorter than    1379 bp
  65% of sequence in fragments shorter than    1515 bp
  70% of sequence in fragments shorter than    1671 bp
  75% of sequence in fragments shorter than    1850 bp
  80% of sequence in fragments shorter than    2065 bp
  85% of sequence in fragments shorter than    2316 bp
  90% of sequence in fragments shorter than    2698 bp
  95% of sequence in fragments shorter than    3357 bp

# SfeI.StuI (CTRYAG+AGGCCT) double digest, 4456 thousand fragments (3531009+925729 sites), 668 bp average fragment length
   5% of sequence in fragments shorter than     234 bp
  10% of sequence in fragments shorter than     347 bp
  15% of sequence in fragments shorter than     454 bp
  20% of sequence in fragments shorter than     550 bp
  25% of sequence in fragments shorter than     649 bp
  30% of sequence in fragments shorter than     738 bp
  35% of sequence in fragments shorter than     841 bp
  40% of sequence in fragments shorter than     942 bp
  45% of sequence in fragments shorter than    1049 bp
  50% of sequence in fragments shorter than    1166 bp
  55% of sequence in fragments shorter than    1287 bp
  60% of sequence in fragments shorter than    1421 bp
  65% of sequence in fragments shorter than    1573 bp
  70% of sequence in fragments shorter than    1743 bp
  75% of sequence in fragments shorter than    1940 bp
  80% of sequence in fragments shorter than    2169 bp
  85% of sequence in fragments shorter than    2469 bp
  90% of sequence in fragments shorter than    2884 bp
  95% of sequence in fragments shorter than    3596 bp

# AvaII.BfiI (GGWCC+ACTGGG) double digest, 4453 thousand fragments (2747690+1706594 sites), 669 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     344 bp
  15% of sequence in fragments shorter than     458 bp
  20% of sequence in fragments shorter than     574 bp
  25% of sequence in fragments shorter than     685 bp
  30% of sequence in fragments shorter than     799 bp
  35% of sequence in fragments shorter than     920 bp
  40% of sequence in fragments shorter than    1039 bp
  45% of sequence in fragments shorter than    1177 bp
  50% of sequence in fragments shorter than    1313 bp
  55% of sequence in fragments shorter than    1471 bp
  60% of sequence in fragments shorter than    1644 bp
  65% of sequence in fragments shorter than    1832 bp
  70% of sequence in fragments shorter than    2052 bp
  75% of sequence in fragments shorter than    2309 bp
  80% of sequence in fragments shorter than    2631 bp
  85% of sequence in fragments shorter than    3043 bp
  90% of sequence in fragments shorter than    3634 bp
  95% of sequence in fragments shorter than    4716 bp

# AciI.BsrBI (CCGC+CCGCTC) double digest, 4441 thousand fragments (4206130+235523 sites), 670 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     629 bp
  20% of sequence in fragments shorter than     827 bp
  25% of sequence in fragments shorter than    1023 bp
  30% of sequence in fragments shorter than    1227 bp
  35% of sequence in fragments shorter than    1444 bp
  40% of sequence in fragments shorter than    1680 bp
  45% of sequence in fragments shorter than    1938 bp
  50% of sequence in fragments shorter than    2224 bp
  55% of sequence in fragments shorter than    2538 bp
  60% of sequence in fragments shorter than    2888 bp
  65% of sequence in fragments shorter than    3289 bp
  70% of sequence in fragments shorter than    3758 bp
  75% of sequence in fragments shorter than    4309 bp
  80% of sequence in fragments shorter than    4987 bp
  85% of sequence in fragments shorter than    5863 bp
  90% of sequence in fragments shorter than    7098 bp
  95% of sequence in fragments shorter than    9264 bp

# AciI.KasI (CCGC+GGCGCC) double digest, 4436 thousand fragments (4206130+230381 sites), 671 bp average fragment length
   5% of sequence in fragments shorter than     210 bp
  10% of sequence in fragments shorter than     414 bp
  15% of sequence in fragments shorter than     608 bp
  20% of sequence in fragments shorter than     801 bp
  25% of sequence in fragments shorter than     993 bp
  30% of sequence in fragments shorter than    1195 bp
  35% of sequence in fragments shorter than    1406 bp
  40% of sequence in fragments shorter than    1637 bp
  45% of sequence in fragments shorter than    1894 bp
  50% of sequence in fragments shorter than    2173 bp
  55% of sequence in fragments shorter than    2481 bp
  60% of sequence in fragments shorter than    2825 bp
  65% of sequence in fragments shorter than    3220 bp
  70% of sequence in fragments shorter than    3681 bp
  75% of sequence in fragments shorter than    4225 bp
  80% of sequence in fragments shorter than    4891 bp
  85% of sequence in fragments shorter than    5752 bp
  90% of sequence in fragments shorter than    6969 bp
  95% of sequence in fragments shorter than    9099 bp

# PstI.StyI (CTGCAG+CCWWGG) double digest, 4435 thousand fragments (1321469+3114107 sites), 671 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     343 bp
  15% of sequence in fragments shorter than     455 bp
  20% of sequence in fragments shorter than     559 bp
  25% of sequence in fragments shorter than     664 bp
  30% of sequence in fragments shorter than     764 bp
  35% of sequence in fragments shorter than     875 bp
  40% of sequence in fragments shorter than     993 bp
  45% of sequence in fragments shorter than    1117 bp
  50% of sequence in fragments shorter than    1249 bp
  55% of sequence in fragments shorter than    1388 bp
  60% of sequence in fragments shorter than    1550 bp
  65% of sequence in fragments shorter than    1731 bp
  70% of sequence in fragments shorter than    1938 bp
  75% of sequence in fragments shorter than    2179 bp
  80% of sequence in fragments shorter than    2474 bp
  85% of sequence in fragments shorter than    2849 bp
  90% of sequence in fragments shorter than    3343 bp
  95% of sequence in fragments shorter than    4316 bp

# BfiI.SmlI (ACTGGG+CTYRAG) double digest, 4433 thousand fragments (1706594+2727867 sites), 672 bp average fragment length
   5% of sequence in fragments shorter than     228 bp
  10% of sequence in fragments shorter than     349 bp
  15% of sequence in fragments shorter than     456 bp
  20% of sequence in fragments shorter than     556 bp
  25% of sequence in fragments shorter than     652 bp
  30% of sequence in fragments shorter than     748 bp
  35% of sequence in fragments shorter than     849 bp
  40% of sequence in fragments shorter than     950 bp
  45% of sequence in fragments shorter than    1056 bp
  50% of sequence in fragments shorter than    1171 bp
  55% of sequence in fragments shorter than    1287 bp
  60% of sequence in fragments shorter than    1406 bp
  65% of sequence in fragments shorter than    1556 bp
  70% of sequence in fragments shorter than    1724 bp
  75% of sequence in fragments shorter than    1921 bp
  80% of sequence in fragments shorter than    2155 bp
  85% of sequence in fragments shorter than    2468 bp
  90% of sequence in fragments shorter than    2908 bp
  95% of sequence in fragments shorter than    3728 bp

# NdeI.SfeI (CATATG+CTRYAG) double digest, 4433 thousand fragments (903360+3531009 sites), 672 bp average fragment length
   5% of sequence in fragments shorter than     234 bp
  10% of sequence in fragments shorter than     351 bp
  15% of sequence in fragments shorter than     456 bp
  20% of sequence in fragments shorter than     552 bp
  25% of sequence in fragments shorter than     648 bp
  30% of sequence in fragments shorter than     739 bp
  35% of sequence in fragments shorter than     835 bp
  40% of sequence in fragments shorter than     931 bp
  45% of sequence in fragments shorter than    1033 bp
  50% of sequence in fragments shorter than    1139 bp
  55% of sequence in fragments shorter than    1250 bp
  60% of sequence in fragments shorter than    1377 bp
  65% of sequence in fragments shorter than    1513 bp
  70% of sequence in fragments shorter than    1671 bp
  75% of sequence in fragments shorter than    1853 bp
  80% of sequence in fragments shorter than    2055 bp
  85% of sequence in fragments shorter than    2325 bp
  90% of sequence in fragments shorter than    2712 bp
  95% of sequence in fragments shorter than    3379 bp

# AciI.SwaI (CCGC+ATTTAAAT) double digest, 4432 thousand fragments (4206130+226602 sites), 672 bp average fragment length
   5% of sequence in fragments shorter than     213 bp
  10% of sequence in fragments shorter than     406 bp
  15% of sequence in fragments shorter than     583 bp
  20% of sequence in fragments shorter than     758 bp
  25% of sequence in fragments shorter than     929 bp
  30% of sequence in fragments shorter than    1104 bp
  35% of sequence in fragments shorter than    1294 bp
  40% of sequence in fragments shorter than    1487 bp
  45% of sequence in fragments shorter than    1705 bp
  50% of sequence in fragments shorter than    1936 bp
  55% of sequence in fragments shorter than    2193 bp
  60% of sequence in fragments shorter than    2474 bp
  65% of sequence in fragments shorter than    2791 bp
  70% of sequence in fragments shorter than    3160 bp
  75% of sequence in fragments shorter than    3595 bp
  80% of sequence in fragments shorter than    4119 bp
  85% of sequence in fragments shorter than    4799 bp
  90% of sequence in fragments shorter than    5753 bp
  95% of sequence in fragments shorter than    7418 bp

# ApaLI.MlyI (GTGCAC+GAGTC) double digest, 4427 thousand fragments (496255+3931692 sites), 673 bp average fragment length
   5% of sequence in fragments shorter than     232 bp
  10% of sequence in fragments shorter than     348 bp
  15% of sequence in fragments shorter than     452 bp
  20% of sequence in fragments shorter than     550 bp
  25% of sequence in fragments shorter than     646 bp
  30% of sequence in fragments shorter than     743 bp
  35% of sequence in fragments shorter than     843 bp
  40% of sequence in fragments shorter than     944 bp
  45% of sequence in fragments shorter than    1052 bp
  50% of sequence in fragments shorter than    1168 bp
  55% of sequence in fragments shorter than    1296 bp
  60% of sequence in fragments shorter than    1434 bp
  65% of sequence in fragments shorter than    1590 bp
  70% of sequence in fragments shorter than    1765 bp
  75% of sequence in fragments shorter than    1973 bp
  80% of sequence in fragments shorter than    2232 bp
  85% of sequence in fragments shorter than    2579 bp
  90% of sequence in fragments shorter than    3047 bp
  95% of sequence in fragments shorter than    3934 bp

# BglII.BscAI (AGATCT+GCATC) double digest, 4427 thousand fragments (774732+3652926 sites), 673 bp average fragment length
   5% of sequence in fragments shorter than     230 bp
  10% of sequence in fragments shorter than     352 bp
  15% of sequence in fragments shorter than     462 bp
  20% of sequence in fragments shorter than     564 bp
  25% of sequence in fragments shorter than     659 bp
  30% of sequence in fragments shorter than     757 bp
  35% of sequence in fragments shorter than     856 bp
  40% of sequence in fragments shorter than     962 bp
  45% of sequence in fragments shorter than    1072 bp
  50% of sequence in fragments shorter than    1189 bp
  55% of sequence in fragments shorter than    1314 bp
  60% of sequence in fragments shorter than    1444 bp
  65% of sequence in fragments shorter than    1595 bp
  70% of sequence in fragments shorter than    1766 bp
  75% of sequence in fragments shorter than    1966 bp
  80% of sequence in fragments shorter than    2209 bp
  85% of sequence in fragments shorter than    2521 bp
  90% of sequence in fragments shorter than    2965 bp
  95% of sequence in fragments shorter than    3784 bp

# NspI.PstI (RCATGY+CTGCAG) double digest, 4425 thousand fragments (3104359+1321469 sites), 673 bp average fragment length
   5% of sequence in fragments shorter than     231 bp
  10% of sequence in fragments shorter than     357 bp
  15% of sequence in fragments shorter than     464 bp
  20% of sequence in fragments shorter than     567 bp
  25% of sequence in fragments shorter than     667 bp
  30% of sequence in fragments shorter than     764 bp
  35% of sequence in fragments shorter than     866 bp
  40% of sequence in fragments shorter than     972 bp
  45% of sequence in fragments shorter than    1082 bp
  50% of sequence in fragments shorter than    1199 bp
  55% of sequence in fragments shorter than    1325 bp
  60% of sequence in fragments shorter than    1460 bp
  65% of sequence in fragments shorter than    1614 bp
  70% of sequence in fragments shorter than    1789 bp
  75% of sequence in fragments shorter than    1992 bp
  80% of sequence in fragments shorter than    2236 bp
  85% of sequence in fragments shorter than    2558 bp
  90% of sequence in fragments shorter than    3005 bp
  95% of sequence in fragments shorter than    3775 bp

# SpeI.Tsp45I (ACTAGT+GTSAC) double digest, 4421 thousand fragments (400286+4021761 sites), 673 bp average fragment length
   5% of sequence in fragments shorter than     237 bp
  10% of sequence in fragments shorter than     353 bp
  15% of sequence in fragments shorter than     458 bp
  20% of sequence in fragments shorter than     557 bp
  25% of sequence in fragments shorter than     654 bp
  30% of sequence in fragments shorter than     749 bp
  35% of sequence in fragments shorter than     848 bp
  40% of sequence in fragments shorter than     948 bp
  45% of sequence in fragments shorter than    1055 bp
  50% of sequence in fragments shorter than    1169 bp
  55% of sequence in fragments shorter than    1291 bp
  60% of sequence in fragments shorter than    1426 bp
  65% of sequence in fragments shorter than    1577 bp
  70% of sequence in fragments shorter than    1750 bp
  75% of sequence in fragments shorter than    1952 bp
  80% of sequence in fragments shorter than    2200 bp
  85% of sequence in fragments shorter than    2518 bp
  90% of sequence in fragments shorter than    2978 bp
  95% of sequence in fragments shorter than    3732 bp

# BscAI.NcoI (GCATC+CCATGG) double digest, 4412 thousand fragments (3652926+759594 sites), 675 bp average fragment length
   5% of sequence in fragments shorter than     230 bp
  10% of sequence in fragments shorter than     353 bp
  15% of sequence in fragments shorter than     466 bp
  20% of sequence in fragments shorter than     571 bp
  25% of sequence in fragments shorter than     671 bp
  30% of sequence in fragments shorter than     772 bp
  35% of sequence in fragments shorter than     874 bp
  40% of sequence in fragments shorter than     984 bp
  45% of sequence in fragments shorter than    1094 bp
  50% of sequence in fragments shorter than    1213 bp
  55% of sequence in fragments shorter than    1343 bp
  60% of sequence in fragments shorter than    1486 bp
  65% of sequence in fragments shorter than    1639 bp
  70% of sequence in fragments shorter than    1818 bp
  75% of sequence in fragments shorter than    2029 bp
  80% of sequence in fragments shorter than    2283 bp
  85% of sequence in fragments shorter than    2614 bp
  90% of sequence in fragments shorter than    3088 bp
  95% of sequence in fragments shorter than    3947 bp

# HpaI.Tsp45I (GTTAAC+GTSAC) double digest, 4410 thousand fragments (389418+4021761 sites), 675 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     350 bp
  15% of sequence in fragments shorter than     455 bp
  20% of sequence in fragments shorter than     555 bp
  25% of sequence in fragments shorter than     651 bp
  30% of sequence in fragments shorter than     745 bp
  35% of sequence in fragments shorter than     846 bp
  40% of sequence in fragments shorter than     947 bp
  45% of sequence in fragments shorter than    1055 bp
  50% of sequence in fragments shorter than    1171 bp
  55% of sequence in fragments shorter than    1295 bp
  60% of sequence in fragments shorter than    1432 bp
  65% of sequence in fragments shorter than    1587 bp
  70% of sequence in fragments shorter than    1762 bp
  75% of sequence in fragments shorter than    1969 bp
  80% of sequence in fragments shorter than    2226 bp
  85% of sequence in fragments shorter than    2562 bp
  90% of sequence in fragments shorter than    3051 bp
  95% of sequence in fragments shorter than    3946 bp

# SapI.Tsp45I (GCTCTTC+GTSAC) double digest, 4398 thousand fragments (377161+4021761 sites), 677 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     349 bp
  15% of sequence in fragments shorter than     456 bp
  20% of sequence in fragments shorter than     555 bp
  25% of sequence in fragments shorter than     655 bp
  30% of sequence in fragments shorter than     751 bp
  35% of sequence in fragments shorter than     854 bp
  40% of sequence in fragments shorter than     957 bp
  45% of sequence in fragments shorter than    1068 bp
  50% of sequence in fragments shorter than    1187 bp
  55% of sequence in fragments shorter than    1313 bp
  60% of sequence in fragments shorter than    1452 bp
  65% of sequence in fragments shorter than    1612 bp
  70% of sequence in fragments shorter than    1794 bp
  75% of sequence in fragments shorter than    2007 bp
  80% of sequence in fragments shorter than    2266 bp
  85% of sequence in fragments shorter than    2611 bp
  90% of sequence in fragments shorter than    3113 bp
  95% of sequence in fragments shorter than    4012 bp

# SmaI.Tsp45I (CCCGGG+GTSAC) double digest, 4398 thousand fragments (376940+4021761 sites), 677 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     350 bp
  15% of sequence in fragments shorter than     456 bp
  20% of sequence in fragments shorter than     557 bp
  25% of sequence in fragments shorter than     655 bp
  30% of sequence in fragments shorter than     753 bp
  35% of sequence in fragments shorter than     857 bp
  40% of sequence in fragments shorter than     961 bp
  45% of sequence in fragments shorter than    1073 bp
  50% of sequence in fragments shorter than    1195 bp
  55% of sequence in fragments shorter than    1325 bp
  60% of sequence in fragments shorter than    1470 bp
  65% of sequence in fragments shorter than    1634 bp
  70% of sequence in fragments shorter than    1820 bp
  75% of sequence in fragments shorter than    2042 bp
  80% of sequence in fragments shorter than    2315 bp
  85% of sequence in fragments shorter than    2681 bp
  90% of sequence in fragments shorter than    3214 bp
  95% of sequence in fragments shorter than    4194 bp

# AvaII.PsiI (GGWCC+TTATAA) double digest, 4395 thousand fragments (2747690+1647912 sites), 678 bp average fragment length
   5% of sequence in fragments shorter than     229 bp
  10% of sequence in fragments shorter than     350 bp
  15% of sequence in fragments shorter than     464 bp
  20% of sequence in fragments shorter than     569 bp
  25% of sequence in fragments shorter than     673 bp
  30% of sequence in fragments shorter than     770 bp
  35% of sequence in fragments shorter than     875 bp
  40% of sequence in fragments shorter than     978 bp
  45% of sequence in fragments shorter than    1092 bp
  50% of sequence in fragments shorter than    1215 bp
  55% of sequence in fragments shorter than    1341 bp
  60% of sequence in fragments shorter than    1483 bp
  65% of sequence in fragments shorter than    1641 bp
  70% of sequence in fragments shorter than    1814 bp
  75% of sequence in fragments shorter than    2017 bp
  80% of sequence in fragments shorter than    2268 bp
  85% of sequence in fragments shorter than    2592 bp
  90% of sequence in fragments shorter than    3052 bp
  95% of sequence in fragments shorter than    3900 bp

# ApaI.MlyI (GGGCCC+GAGTC) double digest, 4391 thousand fragments (460339+3931692 sites), 678 bp average fragment length
   5% of sequence in fragments shorter than     231 bp
  10% of sequence in fragments shorter than     347 bp
  15% of sequence in fragments shorter than     453 bp
  20% of sequence in fragments shorter than     554 bp
  25% of sequence in fragments shorter than     654 bp
  30% of sequence in fragments shorter than     755 bp
  35% of sequence in fragments shorter than     860 bp
  40% of sequence in fragments shorter than     967 bp
  45% of sequence in fragments shorter than    1081 bp
  50% of sequence in fragments shorter than    1206 bp
  55% of sequence in fragments shorter than    1342 bp
  60% of sequence in fragments shorter than    1492 bp
  65% of sequence in fragments shorter than    1662 bp
  70% of sequence in fragments shorter than    1851 bp
  75% of sequence in fragments shorter than    2080 bp
  80% of sequence in fragments shorter than    2371 bp
  85% of sequence in fragments shorter than    2757 bp
  90% of sequence in fragments shorter than    3306 bp
  95% of sequence in fragments shorter than    4352 bp

# BclI.BscAI (TGATCA+GCATC) double digest, 4391 thousand fragments (738785+3652926 sites), 678 bp average fragment length
   5% of sequence in fragments shorter than     232 bp
  10% of sequence in fragments shorter than     359 bp
  15% of sequence in fragments shorter than     471 bp
  20% of sequence in fragments shorter than     575 bp
  25% of sequence in fragments shorter than     673 bp
  30% of sequence in fragments shorter than     770 bp
  35% of sequence in fragments shorter than     870 bp
  40% of sequence in fragments shorter than     977 bp
  45% of sequence in fragments shorter than    1090 bp
  50% of sequence in fragments shorter than    1207 bp
  55% of sequence in fragments shorter than    1331 bp
  60% of sequence in fragments shorter than    1466 bp
  65% of sequence in fragments shorter than    1612 bp
  70% of sequence in fragments shorter than    1783 bp
  75% of sequence in fragments shorter than    1982 bp
  80% of sequence in fragments shorter than    2226 bp
  85% of sequence in fragments shorter than    2537 bp
  90% of sequence in fragments shorter than    2976 bp
  95% of sequence in fragments shorter than    3783 bp

# HindIII.SfeI (AAGCTT+CTRYAG) double digest, 4390 thousand fragments (860365+3531009 sites), 678 bp average fragment length
   5% of sequence in fragments shorter than     240 bp
  10% of sequence in fragments shorter than     357 bp
  15% of sequence in fragments shorter than     461 bp
  20% of sequence in fragments shorter than     555 bp
  25% of sequence in fragments shorter than     653 bp
  30% of sequence in fragments shorter than     745 bp
  35% of sequence in fragments shorter than     841 bp
  40% of sequence in fragments shorter than     942 bp
  45% of sequence in fragments shorter than    1037 bp
  50% of sequence in fragments shorter than    1145 bp
  55% of sequence in fragments shorter than    1261 bp
  60% of sequence in fragments shorter than    1384 bp
  65% of sequence in fragments shorter than    1523 bp
  70% of sequence in fragments shorter than    1683 bp
  75% of sequence in fragments shorter than    1868 bp
  80% of sequence in fragments shorter than    2084 bp
  85% of sequence in fragments shorter than    2360 bp
  90% of sequence in fragments shorter than    2750 bp
  95% of sequence in fragments shorter than    3418 bp

# BamHI.Tsp45I (GGATCC+GTSAC) double digest, 4387 thousand fragments (365999+4021761 sites), 679 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     352 bp
  15% of sequence in fragments shorter than     458 bp
  20% of sequence in fragments shorter than     558 bp
  25% of sequence in fragments shorter than     657 bp
  30% of sequence in fragments shorter than     755 bp
  35% of sequence in fragments shorter than     857 bp
  40% of sequence in fragments shorter than     961 bp
  45% of sequence in fragments shorter than    1071 bp
  50% of sequence in fragments shorter than    1191 bp
  55% of sequence in fragments shorter than    1318 bp
  60% of sequence in fragments shorter than    1457 bp
  65% of sequence in fragments shorter than    1614 bp
  70% of sequence in fragments shorter than    1790 bp
  75% of sequence in fragments shorter than    1998 bp
  80% of sequence in fragments shorter than    2255 bp
  85% of sequence in fragments shorter than    2574 bp
  90% of sequence in fragments shorter than    3038 bp
  95% of sequence in fragments shorter than    3864 bp

# BscAI.FnuDII (GCATC+CGCG) double digest, 4386 thousand fragments (3652926+733940 sites), 679 bp average fragment length
   5% of sequence in fragments shorter than     232 bp
  10% of sequence in fragments shorter than     366 bp
  15% of sequence in fragments shorter than     486 bp
  20% of sequence in fragments shorter than     596 bp
  25% of sequence in fragments shorter than     702 bp
  30% of sequence in fragments shorter than     810 bp
  35% of sequence in fragments shorter than     922 bp
  40% of sequence in fragments shorter than    1039 bp
  45% of sequence in fragments shorter than    1161 bp
  50% of sequence in fragments shorter than    1287 bp
  55% of sequence in fragments shorter than    1423 bp
  60% of sequence in fragments shorter than    1573 bp
  65% of sequence in fragments shorter than    1734 bp
  70% of sequence in fragments shorter than    1922 bp
  75% of sequence in fragments shorter than    2141 bp
  80% of sequence in fragments shorter than    2408 bp
  85% of sequence in fragments shorter than    2751 bp
  90% of sequence in fragments shorter than    3240 bp
  95% of sequence in fragments shorter than    4137 bp

# SfeI.XbaI (CTRYAG+TCTAGA) double digest, 4381 thousand fragments (3531009+850998 sites), 680 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     359 bp
  15% of sequence in fragments shorter than     461 bp
  20% of sequence in fragments shorter than     554 bp
  25% of sequence in fragments shorter than     652 bp
  30% of sequence in fragments shorter than     743 bp
  35% of sequence in fragments shorter than     837 bp
  40% of sequence in fragments shorter than     931 bp
  45% of sequence in fragments shorter than    1035 bp
  50% of sequence in fragments shorter than    1141 bp
  55% of sequence in fragments shorter than    1259 bp
  60% of sequence in fragments shorter than    1383 bp
  65% of sequence in fragments shorter than    1523 bp
  70% of sequence in fragments shorter than    1680 bp
  75% of sequence in fragments shorter than    1859 bp
  80% of sequence in fragments shorter than    2075 bp
  85% of sequence in fragments shorter than    2350 bp
  90% of sequence in fragments shorter than    2735 bp
  95% of sequence in fragments shorter than    3392 bp

# AciI.AclI (CCGC+AACGTT) double digest, 4378 thousand fragments (4206130+172706 sites), 680 bp average fragment length
   5% of sequence in fragments shorter than     212 bp
  10% of sequence in fragments shorter than     408 bp
  15% of sequence in fragments shorter than     591 bp
  20% of sequence in fragments shorter than     769 bp
  25% of sequence in fragments shorter than     949 bp
  30% of sequence in fragments shorter than    1138 bp
  35% of sequence in fragments shorter than    1342 bp
  40% of sequence in fragments shorter than    1549 bp
  45% of sequence in fragments shorter than    1783 bp
  50% of sequence in fragments shorter than    2037 bp
  55% of sequence in fragments shorter than    2316 bp
  60% of sequence in fragments shorter than    2628 bp
  65% of sequence in fragments shorter than    2979 bp
  70% of sequence in fragments shorter than    3389 bp
  75% of sequence in fragments shorter than    3874 bp
  80% of sequence in fragments shorter than    4463 bp
  85% of sequence in fragments shorter than    5235 bp
  90% of sequence in fragments shorter than    6322 bp
  95% of sequence in fragments shorter than    8178 bp

# EcoRI.SfeI (GAATTC+CTRYAG) double digest, 4377 thousand fragments (847401+3531009 sites), 680 bp average fragment length
   5% of sequence in fragments shorter than     240 bp
  10% of sequence in fragments shorter than     355 bp
  15% of sequence in fragments shorter than     462 bp
  20% of sequence in fragments shorter than     559 bp
  25% of sequence in fragments shorter than     657 bp
  30% of sequence in fragments shorter than     747 bp
  35% of sequence in fragments shorter than     847 bp
  40% of sequence in fragments shorter than     944 bp
  45% of sequence in fragments shorter than    1049 bp
  50% of sequence in fragments shorter than    1158 bp
  55% of sequence in fragments shorter than    1270 bp
  60% of sequence in fragments shorter than    1394 bp
  65% of sequence in fragments shorter than    1537 bp
  70% of sequence in fragments shorter than    1697 bp
  75% of sequence in fragments shorter than    1879 bp
  80% of sequence in fragments shorter than    2096 bp
  85% of sequence in fragments shorter than    2369 bp
  90% of sequence in fragments shorter than    2754 bp
  95% of sequence in fragments shorter than    3399 bp

# EcoRV.MlyI (GATATC+GAGTC) double digest, 4377 thousand fragments (446473+3931692 sites), 680 bp average fragment length
   5% of sequence in fragments shorter than     239 bp
  10% of sequence in fragments shorter than     354 bp
  15% of sequence in fragments shorter than     458 bp
  20% of sequence in fragments shorter than     557 bp
  25% of sequence in fragments shorter than     652 bp
  30% of sequence in fragments shorter than     746 bp
  35% of sequence in fragments shorter than     841 bp
  40% of sequence in fragments shorter than     940 bp
  45% of sequence in fragments shorter than    1046 bp
  50% of sequence in fragments shorter than    1160 bp
  55% of sequence in fragments shorter than    1283 bp
  60% of sequence in fragments shorter than    1417 bp
  65% of sequence in fragments shorter than    1568 bp
  70% of sequence in fragments shorter than    1735 bp
  75% of sequence in fragments shorter than    1934 bp
  80% of sequence in fragments shorter than    2176 bp
  85% of sequence in fragments shorter than    2502 bp
  90% of sequence in fragments shorter than    2950 bp
  95% of sequence in fragments shorter than    3773 bp

# PsiI.SmlI (TTATAA+CTYRAG) double digest, 4375 thousand fragments (1647912+2727867 sites), 681 bp average fragment length
   5% of sequence in fragments shorter than     230 bp
  10% of sequence in fragments shorter than     354 bp
  15% of sequence in fragments shorter than     461 bp
  20% of sequence in fragments shorter than     560 bp
  25% of sequence in fragments shorter than     655 bp
  30% of sequence in fragments shorter than     751 bp
  35% of sequence in fragments shorter than     851 bp
  40% of sequence in fragments shorter than     954 bp
  45% of sequence in fragments shorter than    1059 bp
  50% of sequence in fragments shorter than    1169 bp
  55% of sequence in fragments shorter than    1293 bp
  60% of sequence in fragments shorter than    1424 bp
  65% of sequence in fragments shorter than    1566 bp
  70% of sequence in fragments shorter than    1737 bp
  75% of sequence in fragments shorter than    1936 bp
  80% of sequence in fragments shorter than    2173 bp
  85% of sequence in fragments shorter than    2489 bp
  90% of sequence in fragments shorter than    2933 bp
  95% of sequence in fragments shorter than    3807 bp

# BsaAI.BscAI (YACGTR+GCATC) double digest, 4366 thousand fragments (713801+3652926 sites), 682 bp average fragment length
   5% of sequence in fragments shorter than     233 bp
  10% of sequence in fragments shorter than     360 bp
  15% of sequence in fragments shorter than     474 bp
  20% of sequence in fragments shorter than     579 bp
  25% of sequence in fragments shorter than     678 bp
  30% of sequence in fragments shorter than     778 bp
  35% of sequence in fragments shorter than     881 bp
  40% of sequence in fragments shorter than     989 bp
  45% of sequence in fragments shorter than    1097 bp
  50% of sequence in fragments shorter than    1215 bp
  55% of sequence in fragments shorter than    1342 bp
  60% of sequence in fragments shorter than    1476 bp
  65% of sequence in fragments shorter than    1628 bp
  70% of sequence in fragments shorter than    1803 bp
  75% of sequence in fragments shorter than    2003 bp
  80% of sequence in fragments shorter than    2246 bp
  85% of sequence in fragments shorter than    2560 bp
  90% of sequence in fragments shorter than    2994 bp
  95% of sequence in fragments shorter than    3774 bp

# AciI.PacI (CCGC+TTAATTAA) double digest, 4365 thousand fragments (4206130+159455 sites), 682 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     413 bp
  15% of sequence in fragments shorter than     598 bp
  20% of sequence in fragments shorter than     780 bp
  25% of sequence in fragments shorter than     959 bp
  30% of sequence in fragments shorter than    1145 bp
  35% of sequence in fragments shorter than    1346 bp
  40% of sequence in fragments shorter than    1550 bp
  45% of sequence in fragments shorter than    1781 bp
  50% of sequence in fragments shorter than    2030 bp
  55% of sequence in fragments shorter than    2303 bp
  60% of sequence in fragments shorter than    2608 bp
  65% of sequence in fragments shorter than    2952 bp
  70% of sequence in fragments shorter than    3352 bp
  75% of sequence in fragments shorter than    3827 bp
  80% of sequence in fragments shorter than    4398 bp
  85% of sequence in fragments shorter than    5133 bp
  90% of sequence in fragments shorter than    6187 bp
  95% of sequence in fragments shorter than    7989 bp

# AciI.Hpy99I (CCGC+CGWCG) double digest, 4363 thousand fragments (4206130+157917 sites), 682 bp average fragment length
   5% of sequence in fragments shorter than     212 bp
  10% of sequence in fragments shorter than     415 bp
  15% of sequence in fragments shorter than     607 bp
  20% of sequence in fragments shorter than     799 bp
  25% of sequence in fragments shorter than     989 bp
  30% of sequence in fragments shorter than    1189 bp
  35% of sequence in fragments shorter than    1398 bp
  40% of sequence in fragments shorter than    1627 bp
  45% of sequence in fragments shorter than    1880 bp
  50% of sequence in fragments shorter than    2155 bp
  55% of sequence in fragments shorter than    2459 bp
  60% of sequence in fragments shorter than    2800 bp
  65% of sequence in fragments shorter than    3192 bp
  70% of sequence in fragments shorter than    3644 bp
  75% of sequence in fragments shorter than    4180 bp
  80% of sequence in fragments shorter than    4834 bp
  85% of sequence in fragments shorter than    5687 bp
  90% of sequence in fragments shorter than    6887 bp
  95% of sequence in fragments shorter than    8971 bp

# BceAI.BscAI (ACGGC+GCATC) double digest, 4357 thousand fragments (705178+3652926 sites), 683 bp average fragment length
   5% of sequence in fragments shorter than     230 bp
  10% of sequence in fragments shorter than     359 bp
  15% of sequence in fragments shorter than     474 bp
  20% of sequence in fragments shorter than     581 bp
  25% of sequence in fragments shorter than     683 bp
  30% of sequence in fragments shorter than     787 bp
  35% of sequence in fragments shorter than     895 bp
  40% of sequence in fragments shorter than    1010 bp
  45% of sequence in fragments shorter than    1130 bp
  50% of sequence in fragments shorter than    1253 bp
  55% of sequence in fragments shorter than    1385 bp
  60% of sequence in fragments shorter than    1533 bp
  65% of sequence in fragments shorter than    1690 bp
  70% of sequence in fragments shorter than    1876 bp
  75% of sequence in fragments shorter than    2091 bp
  80% of sequence in fragments shorter than    2355 bp
  85% of sequence in fragments shorter than    2693 bp
  90% of sequence in fragments shorter than    3168 bp
  95% of sequence in fragments shorter than    4028 bp

# BstEII.Tsp45I (GGTNACC+GTSAC) double digest, 4357 thousand fragments (335865+4021761 sites), 683 bp average fragment length
   5% of sequence in fragments shorter than     248 bp
  10% of sequence in fragments shorter than     370 bp
  15% of sequence in fragments shorter than     484 bp
  20% of sequence in fragments shorter than     589 bp
  25% of sequence in fragments shorter than     692 bp
  30% of sequence in fragments shorter than     796 bp
  35% of sequence in fragments shorter than     900 bp
  40% of sequence in fragments shorter than    1012 bp
  45% of sequence in fragments shorter than    1130 bp
  50% of sequence in fragments shorter than    1253 bp
  55% of sequence in fragments shorter than    1384 bp
  60% of sequence in fragments shorter than    1535 bp
  65% of sequence in fragments shorter than    1703 bp
  70% of sequence in fragments shorter than    1894 bp
  75% of sequence in fragments shorter than    2119 bp
  80% of sequence in fragments shorter than    2396 bp
  85% of sequence in fragments shorter than    2762 bp
  90% of sequence in fragments shorter than    3298 bp
  95% of sequence in fragments shorter than    4260 bp

# AvaII.EsaBC3I (GGWCC+TCGA) double digest, 4350 thousand fragments (2747690+1603346 sites), 684 bp average fragment length
   5% of sequence in fragments shorter than     227 bp
  10% of sequence in fragments shorter than     348 bp
  15% of sequence in fragments shorter than     462 bp
  20% of sequence in fragments shorter than     572 bp
  25% of sequence in fragments shorter than     683 bp
  30% of sequence in fragments shorter than     792 bp
  35% of sequence in fragments shorter than     907 bp
  40% of sequence in fragments shorter than    1025 bp
  45% of sequence in fragments shorter than    1156 bp
  50% of sequence in fragments shorter than    1296 bp
  55% of sequence in fragments shorter than    1444 bp
  60% of sequence in fragments shorter than    1611 bp
  65% of sequence in fragments shorter than    1797 bp
  70% of sequence in fragments shorter than    2006 bp
  75% of sequence in fragments shorter than    2253 bp
  80% of sequence in fragments shorter than    2553 bp
  85% of sequence in fragments shorter than    2938 bp
  90% of sequence in fragments shorter than    3488 bp
  95% of sequence in fragments shorter than    4449 bp

# BciVI.BscAI (GTATCC+GCATC) double digest, 4348 thousand fragments (696093+3652926 sites), 685 bp average fragment length
   5% of sequence in fragments shorter than     229 bp
  10% of sequence in fragments shorter than     358 bp
  15% of sequence in fragments shorter than     469 bp
  20% of sequence in fragments shorter than     574 bp
  25% of sequence in fragments shorter than     678 bp
  30% of sequence in fragments shorter than     779 bp
  35% of sequence in fragments shorter than     883 bp
  40% of sequence in fragments shorter than     993 bp
  45% of sequence in fragments shorter than    1107 bp
  50% of sequence in fragments shorter than    1224 bp
  55% of sequence in fragments shorter than    1352 bp
  60% of sequence in fragments shorter than    1490 bp
  65% of sequence in fragments shorter than    1642 bp
  70% of sequence in fragments shorter than    1818 bp
  75% of sequence in fragments shorter than    2021 bp
  80% of sequence in fragments shorter than    2264 bp
  85% of sequence in fragments shorter than    2584 bp
  90% of sequence in fragments shorter than    3036 bp
  95% of sequence in fragments shorter than    3836 bp

# BscGI.StyI (CCCGT+CCWWGG) double digest, 4344 thousand fragments (1231070+3114107 sites), 685 bp average fragment length
   5% of sequence in fragments shorter than     228 bp
  10% of sequence in fragments shorter than     345 bp
  15% of sequence in fragments shorter than     451 bp
  20% of sequence in fragments shorter than     557 bp
  25% of sequence in fragments shorter than     666 bp
  30% of sequence in fragments shorter than     767 bp
  35% of sequence in fragments shorter than     882 bp
  40% of sequence in fragments shorter than    1004 bp
  45% of sequence in fragments shorter than    1131 bp
  50% of sequence in fragments shorter than    1269 bp
  55% of sequence in fragments shorter than    1419 bp
  60% of sequence in fragments shorter than    1585 bp
  65% of sequence in fragments shorter than    1772 bp
  70% of sequence in fragments shorter than    1987 bp
  75% of sequence in fragments shorter than    2239 bp
  80% of sequence in fragments shorter than    2546 bp
  85% of sequence in fragments shorter than    2924 bp
  90% of sequence in fragments shorter than    3438 bp
  95% of sequence in fragments shorter than    4366 bp

# AciI.SnaBI (CCGC+TACGTA) double digest, 4337 thousand fragments (4206130+132299 sites), 686 bp average fragment length
   5% of sequence in fragments shorter than     215 bp
  10% of sequence in fragments shorter than     415 bp
  15% of sequence in fragments shorter than     602 bp
  20% of sequence in fragments shorter than     786 bp
  25% of sequence in fragments shorter than     968 bp
  30% of sequence in fragments shorter than    1157 bp
  35% of sequence in fragments shorter than    1361 bp
  40% of sequence in fragments shorter than    1566 bp
  45% of sequence in fragments shorter than    1803 bp
  50% of sequence in fragments shorter than    2059 bp
  55% of sequence in fragments shorter than    2340 bp
  60% of sequence in fragments shorter than    2653 bp
  65% of sequence in fragments shorter than    3010 bp
  70% of sequence in fragments shorter than    3425 bp
  75% of sequence in fragments shorter than    3917 bp
  80% of sequence in fragments shorter than    4511 bp
  85% of sequence in fragments shorter than    5278 bp
  90% of sequence in fragments shorter than    6372 bp
  95% of sequence in fragments shorter than    8236 bp

# BscGI.NspI (CCCGT+RCATGY) double digest, 4334 thousand fragments (1231070+3104359 sites), 687 bp average fragment length
   5% of sequence in fragments shorter than     239 bp
  10% of sequence in fragments shorter than     349 bp
  15% of sequence in fragments shorter than     463 bp
  20% of sequence in fragments shorter than     569 bp
  25% of sequence in fragments shorter than     676 bp
  30% of sequence in fragments shorter than     776 bp
  35% of sequence in fragments shorter than     882 bp
  40% of sequence in fragments shorter than     993 bp
  45% of sequence in fragments shorter than    1107 bp
  50% of sequence in fragments shorter than    1228 bp
  55% of sequence in fragments shorter than    1359 bp
  60% of sequence in fragments shorter than    1501 bp
  65% of sequence in fragments shorter than    1659 bp
  70% of sequence in fragments shorter than    1838 bp
  75% of sequence in fragments shorter than    2045 bp
  80% of sequence in fragments shorter than    2294 bp
  85% of sequence in fragments shorter than    2615 bp
  90% of sequence in fragments shorter than    3055 bp
  95% of sequence in fragments shorter than    3767 bp

# MlyI.SpeI (GAGTC+ACTAGT) double digest, 4331 thousand fragments (3931692+400286 sites), 687 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     358 bp
  15% of sequence in fragments shorter than     463 bp
  20% of sequence in fragments shorter than     563 bp
  25% of sequence in fragments shorter than     660 bp
  30% of sequence in fragments shorter than     756 bp
  35% of sequence in fragments shorter than     854 bp
  40% of sequence in fragments shorter than     955 bp
  45% of sequence in fragments shorter than    1061 bp
  50% of sequence in fragments shorter than    1175 bp
  55% of sequence in fragments shorter than    1300 bp
  60% of sequence in fragments shorter than    1434 bp
  65% of sequence in fragments shorter than    1586 bp
  70% of sequence in fragments shorter than    1755 bp
  75% of sequence in fragments shorter than    1955 bp
  80% of sequence in fragments shorter than    2190 bp
  85% of sequence in fragments shorter than    2512 bp
  90% of sequence in fragments shorter than    2971 bp
  95% of sequence in fragments shorter than    3802 bp

# AciI.NaeI (CCGC+GCCGGC) double digest, 4331 thousand fragments (4206130+125692 sites), 688 bp average fragment length
   5% of sequence in fragments shorter than     214 bp
  10% of sequence in fragments shorter than     420 bp
  15% of sequence in fragments shorter than     614 bp
  20% of sequence in fragments shorter than     809 bp
  25% of sequence in fragments shorter than    1002 bp
  30% of sequence in fragments shorter than    1205 bp
  35% of sequence in fragments shorter than    1417 bp
  40% of sequence in fragments shorter than    1650 bp
  45% of sequence in fragments shorter than    1907 bp
  50% of sequence in fragments shorter than    2188 bp
  55% of sequence in fragments shorter than    2497 bp
  60% of sequence in fragments shorter than    2842 bp
  65% of sequence in fragments shorter than    3239 bp
  70% of sequence in fragments shorter than    3701 bp
  75% of sequence in fragments shorter than    4246 bp
  80% of sequence in fragments shorter than    4916 bp
  85% of sequence in fragments shorter than    5779 bp
  90% of sequence in fragments shorter than    7000 bp
  95% of sequence in fragments shorter than    9141 bp

# EsaBC3I.SmlI (TCGA+CTYRAG) double digest, 4330 thousand fragments (1603346+2727867 sites), 688 bp average fragment length
   5% of sequence in fragments shorter than     246 bp
  10% of sequence in fragments shorter than     374 bp
  15% of sequence in fragments shorter than     484 bp
  20% of sequence in fragments shorter than     586 bp
  25% of sequence in fragments shorter than     688 bp
  30% of sequence in fragments shorter than     789 bp
  35% of sequence in fragments shorter than     891 bp
  40% of sequence in fragments shorter than    1001 bp
  45% of sequence in fragments shorter than    1112 bp
  50% of sequence in fragments shorter than    1232 bp
  55% of sequence in fragments shorter than    1361 bp
  60% of sequence in fragments shorter than    1495 bp
  65% of sequence in fragments shorter than    1653 bp
  70% of sequence in fragments shorter than    1829 bp
  75% of sequence in fragments shorter than    2033 bp
  80% of sequence in fragments shorter than    2269 bp
  85% of sequence in fragments shorter than    2580 bp
  90% of sequence in fragments shorter than    3000 bp
  95% of sequence in fragments shorter than    3747 bp

# AciI.XhoI (CCGC+CTCGAG) double digest, 4326 thousand fragments (4206130+121308 sites), 688 bp average fragment length
   5% of sequence in fragments shorter than     213 bp
  10% of sequence in fragments shorter than     415 bp
  15% of sequence in fragments shorter than     605 bp
  20% of sequence in fragments shorter than     793 bp
  25% of sequence in fragments shorter than     980 bp
  30% of sequence in fragments shorter than    1176 bp
  35% of sequence in fragments shorter than    1384 bp
  40% of sequence in fragments shorter than    1610 bp
  45% of sequence in fragments shorter than    1861 bp
  50% of sequence in fragments shorter than    2133 bp
  55% of sequence in fragments shorter than    2436 bp
  60% of sequence in fragments shorter than    2772 bp
  65% of sequence in fragments shorter than    3156 bp
  70% of sequence in fragments shorter than    3605 bp
  75% of sequence in fragments shorter than    4135 bp
  80% of sequence in fragments shorter than    4778 bp
  85% of sequence in fragments shorter than    5622 bp
  90% of sequence in fragments shorter than    6807 bp
  95% of sequence in fragments shorter than    8878 bp

# HpaI.MlyI (GTTAAC+GAGTC) double digest, 4320 thousand fragments (389418+3931692 sites), 689 bp average fragment length
   5% of sequence in fragments shorter than     241 bp
  10% of sequence in fragments shorter than     357 bp
  15% of sequence in fragments shorter than     462 bp
  20% of sequence in fragments shorter than     562 bp
  25% of sequence in fragments shorter than     659 bp
  30% of sequence in fragments shorter than     756 bp
  35% of sequence in fragments shorter than     856 bp
  40% of sequence in fragments shorter than     957 bp
  45% of sequence in fragments shorter than    1065 bp
  50% of sequence in fragments shorter than    1182 bp
  55% of sequence in fragments shorter than    1309 bp
  60% of sequence in fragments shorter than    1448 bp
  65% of sequence in fragments shorter than    1604 bp
  70% of sequence in fragments shorter than    1779 bp
  75% of sequence in fragments shorter than    1988 bp
  80% of sequence in fragments shorter than    2250 bp
  85% of sequence in fragments shorter than    2601 bp
  90% of sequence in fragments shorter than    3101 bp
  95% of sequence in fragments shorter than    4043 bp

# SexAI.Tsp45I (ACCWGGT+GTSAC) double digest, 4319 thousand fragments (297900+4021761 sites), 689 bp average fragment length
   5% of sequence in fragments shorter than     241 bp
  10% of sequence in fragments shorter than     358 bp
  15% of sequence in fragments shorter than     466 bp
  20% of sequence in fragments shorter than     568 bp
  25% of sequence in fragments shorter than     669 bp
  30% of sequence in fragments shorter than     768 bp
  35% of sequence in fragments shorter than     870 bp
  40% of sequence in fragments shorter than     978 bp
  45% of sequence in fragments shorter than    1091 bp
  50% of sequence in fragments shorter than    1213 bp
  55% of sequence in fragments shorter than    1343 bp
  60% of sequence in fragments shorter than    1488 bp
  65% of sequence in fragments shorter than    1651 bp
  70% of sequence in fragments shorter than    1836 bp
  75% of sequence in fragments shorter than    2056 bp
  80% of sequence in fragments shorter than    2326 bp
  85% of sequence in fragments shorter than    2687 bp
  90% of sequence in fragments shorter than    3212 bp
  95% of sequence in fragments shorter than    4176 bp

# KpnI.Tsp45I (GGTACC+GTSAC) double digest, 4309 thousand fragments (287974+4021761 sites), 691 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     360 bp
  15% of sequence in fragments shorter than     469 bp
  20% of sequence in fragments shorter than     570 bp
  25% of sequence in fragments shorter than     671 bp
  30% of sequence in fragments shorter than     771 bp
  35% of sequence in fragments shorter than     871 bp
  40% of sequence in fragments shorter than     979 bp
  45% of sequence in fragments shorter than    1090 bp
  50% of sequence in fragments shorter than    1208 bp
  55% of sequence in fragments shorter than    1329 bp
  60% of sequence in fragments shorter than    1470 bp
  65% of sequence in fragments shorter than    1622 bp
  70% of sequence in fragments shorter than    1794 bp
  75% of sequence in fragments shorter than    1998 bp
  80% of sequence in fragments shorter than    2245 bp
  85% of sequence in fragments shorter than    2566 bp
  90% of sequence in fragments shorter than    3011 bp
  95% of sequence in fragments shorter than    3827 bp

# NheI.Tsp45I (GCTAGC+GTSAC) double digest, 4308 thousand fragments (287384+4021761 sites), 691 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     360 bp
  15% of sequence in fragments shorter than     470 bp
  20% of sequence in fragments shorter than     572 bp
  25% of sequence in fragments shorter than     670 bp
  30% of sequence in fragments shorter than     769 bp
  35% of sequence in fragments shorter than     869 bp
  40% of sequence in fragments shorter than     976 bp
  45% of sequence in fragments shorter than    1086 bp
  50% of sequence in fragments shorter than    1202 bp
  55% of sequence in fragments shorter than    1329 bp
  60% of sequence in fragments shorter than    1468 bp
  65% of sequence in fragments shorter than    1623 bp
  70% of sequence in fragments shorter than    1788 bp
  75% of sequence in fragments shorter than    1983 bp
  80% of sequence in fragments shorter than    2228 bp
  85% of sequence in fragments shorter than    2545 bp
  90% of sequence in fragments shorter than    2990 bp
  95% of sequence in fragments shorter than    3787 bp

# MlyI.SapI (GAGTC+GCTCTTC) double digest, 4308 thousand fragments (3931692+377161 sites), 691 bp average fragment length
   5% of sequence in fragments shorter than     239 bp
  10% of sequence in fragments shorter than     358 bp
  15% of sequence in fragments shorter than     465 bp
  20% of sequence in fragments shorter than     564 bp
  25% of sequence in fragments shorter than     663 bp
  30% of sequence in fragments shorter than     763 bp
  35% of sequence in fragments shorter than     863 bp
  40% of sequence in fragments shorter than     969 bp
  45% of sequence in fragments shorter than    1080 bp
  50% of sequence in fragments shorter than    1198 bp
  55% of sequence in fragments shorter than    1328 bp
  60% of sequence in fragments shorter than    1469 bp
  65% of sequence in fragments shorter than    1632 bp
  70% of sequence in fragments shorter than    1817 bp
  75% of sequence in fragments shorter than    2038 bp
  80% of sequence in fragments shorter than    2312 bp
  85% of sequence in fragments shorter than    2680 bp
  90% of sequence in fragments shorter than    3200 bp
  95% of sequence in fragments shorter than    4182 bp

# MlyI.SmaI (GAGTC+CCCGGG) double digest, 4308 thousand fragments (3931692+376940 sites), 691 bp average fragment length
   5% of sequence in fragments shorter than     237 bp
  10% of sequence in fragments shorter than     358 bp
  15% of sequence in fragments shorter than     468 bp
  20% of sequence in fragments shorter than     573 bp
  25% of sequence in fragments shorter than     676 bp
  30% of sequence in fragments shorter than     778 bp
  35% of sequence in fragments shorter than     883 bp
  40% of sequence in fragments shorter than     995 bp
  45% of sequence in fragments shorter than    1111 bp
  50% of sequence in fragments shorter than    1239 bp
  55% of sequence in fragments shorter than    1377 bp
  60% of sequence in fragments shorter than    1529 bp
  65% of sequence in fragments shorter than    1700 bp
  70% of sequence in fragments shorter than    1892 bp
  75% of sequence in fragments shorter than    2125 bp
  80% of sequence in fragments shorter than    2421 bp
  85% of sequence in fragments shorter than    2809 bp
  90% of sequence in fragments shorter than    3365 bp
  95% of sequence in fragments shorter than    4422 bp

# BglII.SfeI (AGATCT+CTRYAG) double digest, 4305 thousand fragments (774732+3531009 sites), 692 bp average fragment length
   5% of sequence in fragments shorter than     244 bp
  10% of sequence in fragments shorter than     365 bp
  15% of sequence in fragments shorter than     468 bp
  20% of sequence in fragments shorter than     568 bp
  25% of sequence in fragments shorter than     666 bp
  30% of sequence in fragments shorter than     761 bp
  35% of sequence in fragments shorter than     862 bp
  40% of sequence in fragments shorter than     966 bp
  45% of sequence in fragments shorter than    1073 bp
  50% of sequence in fragments shorter than    1189 bp
  55% of sequence in fragments shorter than    1313 bp
  60% of sequence in fragments shorter than    1440 bp
  65% of sequence in fragments shorter than    1587 bp
  70% of sequence in fragments shorter than    1746 bp
  75% of sequence in fragments shorter than    1935 bp
  80% of sequence in fragments shorter than    2154 bp
  85% of sequence in fragments shorter than    2444 bp
  90% of sequence in fragments shorter than    2851 bp
  95% of sequence in fragments shorter than    3567 bp

# AvaII.HgiCI (GGWCC+GGYRCC) double digest, 4298 thousand fragments (2747690+1550877 sites), 693 bp average fragment length
   5% of sequence in fragments shorter than     222 bp
  10% of sequence in fragments shorter than     345 bp
  15% of sequence in fragments shorter than     469 bp
  20% of sequence in fragments shorter than     590 bp
  25% of sequence in fragments shorter than     707 bp
  30% of sequence in fragments shorter than     835 bp
  35% of sequence in fragments shorter than     953 bp
  40% of sequence in fragments shorter than    1091 bp
  45% of sequence in fragments shorter than    1234 bp
  50% of sequence in fragments shorter than    1392 bp
  55% of sequence in fragments shorter than    1559 bp
  60% of sequence in fragments shorter than    1749 bp
  65% of sequence in fragments shorter than    1962 bp
  70% of sequence in fragments shorter than    2208 bp
  75% of sequence in fragments shorter than    2499 bp
  80% of sequence in fragments shorter than    2851 bp
  85% of sequence in fragments shorter than    3319 bp
  90% of sequence in fragments shorter than    4007 bp
  95% of sequence in fragments shorter than    5287 bp

# BamHI.MlyI (GGATCC+GAGTC) double digest, 4297 thousand fragments (365999+3931692 sites), 693 bp average fragment length
   5% of sequence in fragments shorter than     241 bp
  10% of sequence in fragments shorter than     359 bp
  15% of sequence in fragments shorter than     466 bp
  20% of sequence in fragments shorter than     568 bp
  25% of sequence in fragments shorter than     667 bp
  30% of sequence in fragments shorter than     767 bp
  35% of sequence in fragments shorter than     868 bp
  40% of sequence in fragments shorter than     975 bp
  45% of sequence in fragments shorter than    1087 bp
  50% of sequence in fragments shorter than    1208 bp
  55% of sequence in fragments shorter than    1338 bp
  60% of sequence in fragments shorter than    1474 bp
  65% of sequence in fragments shorter than    1634 bp
  70% of sequence in fragments shorter than    1812 bp
  75% of sequence in fragments shorter than    2023 bp
  80% of sequence in fragments shorter than    2281 bp
  85% of sequence in fragments shorter than    2616 bp
  90% of sequence in fragments shorter than    3109 bp
  95% of sequence in fragments shorter than    4051 bp

# AvaII.BsmI (GGWCC+GAATGC) double digest, 4296 thousand fragments (2747690+1549350 sites), 693 bp average fragment length
   5% of sequence in fragments shorter than     211 bp
  10% of sequence in fragments shorter than     346 bp
  15% of sequence in fragments shorter than     476 bp
  20% of sequence in fragments shorter than     595 bp
  25% of sequence in fragments shorter than     719 bp
  30% of sequence in fragments shorter than     843 bp
  35% of sequence in fragments shorter than     970 bp
  40% of sequence in fragments shorter than    1103 bp
  45% of sequence in fragments shorter than    1245 bp
  50% of sequence in fragments shorter than    1395 bp
  55% of sequence in fragments shorter than    1557 bp
  60% of sequence in fragments shorter than    1734 bp
  65% of sequence in fragments shorter than    1928 bp
  70% of sequence in fragments shorter than    2149 bp
  75% of sequence in fragments shorter than    2405 bp
  80% of sequence in fragments shorter than    2710 bp
  85% of sequence in fragments shorter than    3102 bp
  90% of sequence in fragments shorter than    3649 bp
  95% of sequence in fragments shorter than    4584 bp

# AflII.BscAI (CTTAAG+GCATC) double digest, 4295 thousand fragments (643284+3652926 sites), 693 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     365 bp
  15% of sequence in fragments shorter than     475 bp
  20% of sequence in fragments shorter than     581 bp
  25% of sequence in fragments shorter than     686 bp
  30% of sequence in fragments shorter than     786 bp
  35% of sequence in fragments shorter than     892 bp
  40% of sequence in fragments shorter than    1002 bp
  45% of sequence in fragments shorter than    1117 bp
  50% of sequence in fragments shorter than    1234 bp
  55% of sequence in fragments shorter than    1364 bp
  60% of sequence in fragments shorter than    1503 bp
  65% of sequence in fragments shorter than    1650 bp
  70% of sequence in fragments shorter than    1826 bp
  75% of sequence in fragments shorter than    2028 bp
  80% of sequence in fragments shorter than    2273 bp
  85% of sequence in fragments shorter than    2592 bp
  90% of sequence in fragments shorter than    3043 bp
  95% of sequence in fragments shorter than    3881 bp

# AciI.ClaI (CCGC+ATCGAT) double digest, 4292 thousand fragments (4206130+86477 sites), 694 bp average fragment length
   5% of sequence in fragments shorter than     217 bp
  10% of sequence in fragments shorter than     421 bp
  15% of sequence in fragments shorter than     611 bp
  20% of sequence in fragments shorter than     799 bp
  25% of sequence in fragments shorter than     984 bp
  30% of sequence in fragments shorter than    1179 bp
  35% of sequence in fragments shorter than    1383 bp
  40% of sequence in fragments shorter than    1606 bp
  45% of sequence in fragments shorter than    1852 bp
  50% of sequence in fragments shorter than    2116 bp
  55% of sequence in fragments shorter than    2410 bp
  60% of sequence in fragments shorter than    2738 bp
  65% of sequence in fragments shorter than    3109 bp
  70% of sequence in fragments shorter than    3542 bp
  75% of sequence in fragments shorter than    4056 bp
  80% of sequence in fragments shorter than    4682 bp
  85% of sequence in fragments shorter than    5495 bp
  90% of sequence in fragments shorter than    6652 bp
  95% of sequence in fragments shorter than    8627 bp

# NcoI.SfeI (CCATGG+CTRYAG) double digest, 4290 thousand fragments (759594+3531009 sites), 694 bp average fragment length
   5% of sequence in fragments shorter than     243 bp
  10% of sequence in fragments shorter than     365 bp
  15% of sequence in fragments shorter than     471 bp
  20% of sequence in fragments shorter than     573 bp
  25% of sequence in fragments shorter than     666 bp
  30% of sequence in fragments shorter than     764 bp
  35% of sequence in fragments shorter than     868 bp
  40% of sequence in fragments shorter than     973 bp
  45% of sequence in fragments shorter than    1077 bp
  50% of sequence in fragments shorter than    1192 bp
  55% of sequence in fragments shorter than    1318 bp
  60% of sequence in fragments shorter than    1451 bp
  65% of sequence in fragments shorter than    1603 bp
  70% of sequence in fragments shorter than    1775 bp
  75% of sequence in fragments shorter than    1971 bp
  80% of sequence in fragments shorter than    2186 bp
  85% of sequence in fragments shorter than    2489 bp
  90% of sequence in fragments shorter than    2911 bp
  95% of sequence in fragments shorter than    3646 bp

# BscAI.HaeII (GCATC+RGCGCY) double digest, 4282 thousand fragments (3652926+630101 sites), 695 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     360 bp
  15% of sequence in fragments shorter than     477 bp
  20% of sequence in fragments shorter than     587 bp
  25% of sequence in fragments shorter than     688 bp
  30% of sequence in fragments shorter than     790 bp
  35% of sequence in fragments shorter than     899 bp
  40% of sequence in fragments shorter than    1014 bp
  45% of sequence in fragments shorter than    1131 bp
  50% of sequence in fragments shorter than    1255 bp
  55% of sequence in fragments shorter than    1389 bp
  60% of sequence in fragments shorter than    1538 bp
  65% of sequence in fragments shorter than    1693 bp
  70% of sequence in fragments shorter than    1877 bp
  75% of sequence in fragments shorter than    2087 bp
  80% of sequence in fragments shorter than    2341 bp
  85% of sequence in fragments shorter than    2666 bp
  90% of sequence in fragments shorter than    3114 bp
  95% of sequence in fragments shorter than    3870 bp

# HgiCI.SmlI (GGYRCC+CTYRAG) double digest, 4278 thousand fragments (1550877+2727867 sites), 696 bp average fragment length
   5% of sequence in fragments shorter than     232 bp
  10% of sequence in fragments shorter than     356 bp
  15% of sequence in fragments shorter than     465 bp
  20% of sequence in fragments shorter than     566 bp
  25% of sequence in fragments shorter than     668 bp
  30% of sequence in fragments shorter than     765 bp
  35% of sequence in fragments shorter than     869 bp
  40% of sequence in fragments shorter than     977 bp
  45% of sequence in fragments shorter than    1092 bp
  50% of sequence in fragments shorter than    1216 bp
  55% of sequence in fragments shorter than    1349 bp
  60% of sequence in fragments shorter than    1494 bp
  65% of sequence in fragments shorter than    1651 bp
  70% of sequence in fragments shorter than    1837 bp
  75% of sequence in fragments shorter than    2050 bp
  80% of sequence in fragments shorter than    2308 bp
  85% of sequence in fragments shorter than    2656 bp
  90% of sequence in fragments shorter than    3152 bp
  95% of sequence in fragments shorter than    4114 bp

# AatII.AciI (GACGTC+CCGC) double digest, 4276 thousand fragments (71089+4206130 sites), 696 bp average fragment length
   5% of sequence in fragments shorter than     216 bp
  10% of sequence in fragments shorter than     422 bp
  15% of sequence in fragments shorter than     615 bp
  20% of sequence in fragments shorter than     808 bp
  25% of sequence in fragments shorter than     999 bp
  30% of sequence in fragments shorter than    1199 bp
  35% of sequence in fragments shorter than    1409 bp
  40% of sequence in fragments shorter than    1639 bp
  45% of sequence in fragments shorter than    1893 bp
  50% of sequence in fragments shorter than    2170 bp
  55% of sequence in fragments shorter than    2474 bp
  60% of sequence in fragments shorter than    2814 bp
  65% of sequence in fragments shorter than    3204 bp
  70% of sequence in fragments shorter than    3658 bp
  75% of sequence in fragments shorter than    4193 bp
  80% of sequence in fragments shorter than    4847 bp
  85% of sequence in fragments shorter than    5697 bp
  90% of sequence in fragments shorter than    6892 bp
  95% of sequence in fragments shorter than    8975 bp

# BsmI.SmlI (GAATGC+CTYRAG) double digest, 4276 thousand fragments (1549350+2727867 sites), 696 bp average fragment length
   5% of sequence in fragments shorter than     216 bp
  10% of sequence in fragments shorter than     356 bp
  15% of sequence in fragments shorter than     480 bp
  20% of sequence in fragments shorter than     591 bp
  25% of sequence in fragments shorter than     697 bp
  30% of sequence in fragments shorter than     806 bp
  35% of sequence in fragments shorter than     914 bp
  40% of sequence in fragments shorter than    1025 bp
  45% of sequence in fragments shorter than    1140 bp
  50% of sequence in fragments shorter than    1265 bp
  55% of sequence in fragments shorter than    1393 bp
  60% of sequence in fragments shorter than    1539 bp
  65% of sequence in fragments shorter than    1699 bp
  70% of sequence in fragments shorter than    1876 bp
  75% of sequence in fragments shorter than    2080 bp
  80% of sequence in fragments shorter than    2326 bp
  85% of sequence in fragments shorter than    2612 bp
  90% of sequence in fragments shorter than    3024 bp
  95% of sequence in fragments shorter than    3730 bp

# AciI.SacII (CCGC+CCGCGG) double digest, 4272 thousand fragments (4206130+66901 sites), 697 bp average fragment length
   5% of sequence in fragments shorter than     221 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     629 bp
  20% of sequence in fragments shorter than     827 bp
  25% of sequence in fragments shorter than    1023 bp
  30% of sequence in fragments shorter than    1227 bp
  35% of sequence in fragments shorter than    1444 bp
  40% of sequence in fragments shorter than    1680 bp
  45% of sequence in fragments shorter than    1938 bp
  50% of sequence in fragments shorter than    2224 bp
  55% of sequence in fragments shorter than    2538 bp
  60% of sequence in fragments shorter than    2888 bp
  65% of sequence in fragments shorter than    3289 bp
  70% of sequence in fragments shorter than    3758 bp
  75% of sequence in fragments shorter than    4309 bp
  80% of sequence in fragments shorter than    4987 bp
  85% of sequence in fragments shorter than    5864 bp
  90% of sequence in fragments shorter than    7098 bp
  95% of sequence in fragments shorter than    9264 bp

# BclI.SfeI (TGATCA+CTRYAG) double digest, 4269 thousand fragments (738785+3531009 sites), 698 bp average fragment length
   5% of sequence in fragments shorter than     246 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     477 bp
  20% of sequence in fragments shorter than     578 bp
  25% of sequence in fragments shorter than     677 bp
  30% of sequence in fragments shorter than     775 bp
  35% of sequence in fragments shorter than     877 bp
  40% of sequence in fragments shorter than     977 bp
  45% of sequence in fragments shorter than    1084 bp
  50% of sequence in fragments shorter than    1192 bp
  55% of sequence in fragments shorter than    1315 bp
  60% of sequence in fragments shorter than    1442 bp
  65% of sequence in fragments shorter than    1588 bp
  70% of sequence in fragments shorter than    1754 bp
  75% of sequence in fragments shorter than    1942 bp
  80% of sequence in fragments shorter than    2166 bp
  85% of sequence in fragments shorter than    2455 bp
  90% of sequence in fragments shorter than    2862 bp
  95% of sequence in fragments shorter than    3577 bp

# BstEII.MlyI (GGTNACC+GAGTC) double digest, 4267 thousand fragments (335865+3931692 sites), 698 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     361 bp
  15% of sequence in fragments shorter than     466 bp
  20% of sequence in fragments shorter than     568 bp
  25% of sequence in fragments shorter than     668 bp
  30% of sequence in fragments shorter than     768 bp
  35% of sequence in fragments shorter than     868 bp
  40% of sequence in fragments shorter than     976 bp
  45% of sequence in fragments shorter than    1087 bp
  50% of sequence in fragments shorter than    1209 bp
  55% of sequence in fragments shorter than    1339 bp
  60% of sequence in fragments shorter than    1482 bp
  65% of sequence in fragments shorter than    1645 bp
  70% of sequence in fragments shorter than    1833 bp
  75% of sequence in fragments shorter than    2056 bp
  80% of sequence in fragments shorter than    2336 bp
  85% of sequence in fragments shorter than    2712 bp
  90% of sequence in fragments shorter than    3248 bp
  95% of sequence in fragments shorter than    4258 bp

# AciI.PspXI (CCGC+VCTCGAGB) double digest, 4264 thousand fragments (4206130+59193 sites), 698 bp average fragment length
   5% of sequence in fragments shorter than     217 bp
  10% of sequence in fragments shorter than     423 bp
  15% of sequence in fragments shorter than     617 bp
  20% of sequence in fragments shorter than     811 bp
  25% of sequence in fragments shorter than    1003 bp
  30% of sequence in fragments shorter than    1205 bp
  35% of sequence in fragments shorter than    1416 bp
  40% of sequence in fragments shorter than    1647 bp
  45% of sequence in fragments shorter than    1903 bp
  50% of sequence in fragments shorter than    2182 bp
  55% of sequence in fragments shorter than    2489 bp
  60% of sequence in fragments shorter than    2832 bp
  65% of sequence in fragments shorter than    3226 bp
  70% of sequence in fragments shorter than    3686 bp
  75% of sequence in fragments shorter than    4226 bp
  80% of sequence in fragments shorter than    4888 bp
  85% of sequence in fragments shorter than    5749 bp
  90% of sequence in fragments shorter than    6955 bp
  95% of sequence in fragments shorter than    9084 bp

# FnuDII.SfeI (CGCG+CTRYAG) double digest, 4264 thousand fragments (733940+3531009 sites), 698 bp average fragment length
   5% of sequence in fragments shorter than     240 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     496 bp
  20% of sequence in fragments shorter than     610 bp
  25% of sequence in fragments shorter than     718 bp
  30% of sequence in fragments shorter than     832 bp
  35% of sequence in fragments shorter than     947 bp
  40% of sequence in fragments shorter than    1063 bp
  45% of sequence in fragments shorter than    1185 bp
  50% of sequence in fragments shorter than    1318 bp
  55% of sequence in fragments shorter than    1454 bp
  60% of sequence in fragments shorter than    1608 bp
  65% of sequence in fragments shorter than    1778 bp
  70% of sequence in fragments shorter than    1967 bp
  75% of sequence in fragments shorter than    2169 bp
  80% of sequence in fragments shorter than    2436 bp
  85% of sequence in fragments shorter than    2776 bp
  90% of sequence in fragments shorter than    3253 bp
  95% of sequence in fragments shorter than    4098 bp

# AciI.AgeI (CCGC+ACCGGT) double digest, 4259 thousand fragments (4206130+53386 sites), 699 bp average fragment length
   5% of sequence in fragments shorter than     217 bp
  10% of sequence in fragments shorter than     424 bp
  15% of sequence in fragments shorter than     618 bp
  20% of sequence in fragments shorter than     812 bp
  25% of sequence in fragments shorter than    1004 bp
  30% of sequence in fragments shorter than    1204 bp
  35% of sequence in fragments shorter than    1414 bp
  40% of sequence in fragments shorter than    1645 bp
  45% of sequence in fragments shorter than    1900 bp
  50% of sequence in fragments shorter than    2179 bp
  55% of sequence in fragments shorter than    2486 bp
  60% of sequence in fragments shorter than    2827 bp
  65% of sequence in fragments shorter than    3220 bp
  70% of sequence in fragments shorter than    3676 bp
  75% of sequence in fragments shorter than    4213 bp
  80% of sequence in fragments shorter than    4874 bp
  85% of sequence in fragments shorter than    5730 bp
  90% of sequence in fragments shorter than    6935 bp
  95% of sequence in fragments shorter than    9046 bp

# BsrBI.Tsp45I (CCGCTC+GTSAC) double digest, 4256 thousand fragments (235523+4021761 sites), 700 bp average fragment length
   5% of sequence in fragments shorter than     243 bp
  10% of sequence in fragments shorter than     361 bp
  15% of sequence in fragments shorter than     471 bp
  20% of sequence in fragments shorter than     575 bp
  25% of sequence in fragments shorter than     679 bp
  30% of sequence in fragments shorter than     780 bp
  35% of sequence in fragments shorter than     884 bp
  40% of sequence in fragments shorter than     994 bp
  45% of sequence in fragments shorter than    1109 bp
  50% of sequence in fragments shorter than    1232 bp
  55% of sequence in fragments shorter than    1367 bp
  60% of sequence in fragments shorter than    1516 bp
  65% of sequence in fragments shorter than    1683 bp
  70% of sequence in fragments shorter than    1873 bp
  75% of sequence in fragments shorter than    2097 bp
  80% of sequence in fragments shorter than    2374 bp
  85% of sequence in fragments shorter than    2739 bp
  90% of sequence in fragments shorter than    3270 bp
  95% of sequence in fragments shorter than    4202 bp

# BscAI.SacI (GCATC+GAGCTC) double digest, 4255 thousand fragments (3652926+603323 sites), 700 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     367 bp
  15% of sequence in fragments shorter than     483 bp
  20% of sequence in fragments shorter than     592 bp
  25% of sequence in fragments shorter than     692 bp
  30% of sequence in fragments shorter than     797 bp
  35% of sequence in fragments shorter than     907 bp
  40% of sequence in fragments shorter than    1021 bp
  45% of sequence in fragments shorter than    1139 bp
  50% of sequence in fragments shorter than    1262 bp
  55% of sequence in fragments shorter than    1393 bp
  60% of sequence in fragments shorter than    1542 bp
  65% of sequence in fragments shorter than    1696 bp
  70% of sequence in fragments shorter than    1879 bp
  75% of sequence in fragments shorter than    2090 bp
  80% of sequence in fragments shorter than    2353 bp
  85% of sequence in fragments shorter than    2689 bp
  90% of sequence in fragments shorter than    3168 bp
  95% of sequence in fragments shorter than    4040 bp

# KasI.Tsp45I (GGCGCC+GTSAC) double digest, 4251 thousand fragments (230381+4021761 sites), 700 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     360 bp
  15% of sequence in fragments shorter than     471 bp
  20% of sequence in fragments shorter than     573 bp
  25% of sequence in fragments shorter than     676 bp
  30% of sequence in fragments shorter than     777 bp
  35% of sequence in fragments shorter than     880 bp
  40% of sequence in fragments shorter than     990 bp
  45% of sequence in fragments shorter than    1105 bp
  50% of sequence in fragments shorter than    1227 bp
  55% of sequence in fragments shorter than    1362 bp
  60% of sequence in fragments shorter than    1508 bp
  65% of sequence in fragments shorter than    1676 bp
  70% of sequence in fragments shorter than    1866 bp
  75% of sequence in fragments shorter than    2090 bp
  80% of sequence in fragments shorter than    2368 bp
  85% of sequence in fragments shorter than    2737 bp
  90% of sequence in fragments shorter than    3279 bp
  95% of sequence in fragments shorter than    4257 bp

# SwaI.Tsp45I (ATTTAAAT+GTSAC) double digest, 4247 thousand fragments (226602+4021761 sites), 701 bp average fragment length
   5% of sequence in fragments shorter than     246 bp
  10% of sequence in fragments shorter than     364 bp
  15% of sequence in fragments shorter than     473 bp
  20% of sequence in fragments shorter than     575 bp
  25% of sequence in fragments shorter than     676 bp
  30% of sequence in fragments shorter than     775 bp
  35% of sequence in fragments shorter than     876 bp
  40% of sequence in fragments shorter than     984 bp
  45% of sequence in fragments shorter than    1096 bp
  50% of sequence in fragments shorter than    1217 bp
  55% of sequence in fragments shorter than    1346 bp
  60% of sequence in fragments shorter than    1488 bp
  65% of sequence in fragments shorter than    1648 bp
  70% of sequence in fragments shorter than    1830 bp
  75% of sequence in fragments shorter than    2045 bp
  80% of sequence in fragments shorter than    2308 bp
  85% of sequence in fragments shorter than    2656 bp
  90% of sequence in fragments shorter than    3166 bp
  95% of sequence in fragments shorter than    4103 bp

# AciI.PmeI (CCGC+GTTTAAAC) double digest, 4247 thousand fragments (4206130+42094 sites), 701 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     426 bp
  15% of sequence in fragments shorter than     620 bp
  20% of sequence in fragments shorter than     813 bp
  25% of sequence in fragments shorter than    1004 bp
  30% of sequence in fragments shorter than    1204 bp
  35% of sequence in fragments shorter than    1413 bp
  40% of sequence in fragments shorter than    1641 bp
  45% of sequence in fragments shorter than    1894 bp
  50% of sequence in fragments shorter than    2168 bp
  55% of sequence in fragments shorter than    2470 bp
  60% of sequence in fragments shorter than    2805 bp
  65% of sequence in fragments shorter than    3192 bp
  70% of sequence in fragments shorter than    3641 bp
  75% of sequence in fragments shorter than    4170 bp
  80% of sequence in fragments shorter than    4820 bp
  85% of sequence in fragments shorter than    5661 bp
  90% of sequence in fragments shorter than    6849 bp
  95% of sequence in fragments shorter than    8901 bp

# AvrII.BscAI (CCTAGG+GCATC) double digest, 4247 thousand fragments (594956+3652926 sites), 701 bp average fragment length
   5% of sequence in fragments shorter than     240 bp
  10% of sequence in fragments shorter than     360 bp
  15% of sequence in fragments shorter than     477 bp
  20% of sequence in fragments shorter than     586 bp
  25% of sequence in fragments shorter than     692 bp
  30% of sequence in fragments shorter than     798 bp
  35% of sequence in fragments shorter than     908 bp
  40% of sequence in fragments shorter than    1022 bp
  45% of sequence in fragments shorter than    1141 bp
  50% of sequence in fragments shorter than    1262 bp
  55% of sequence in fragments shorter than    1392 bp
  60% of sequence in fragments shorter than    1540 bp
  65% of sequence in fragments shorter than    1694 bp
  70% of sequence in fragments shorter than    1879 bp
  75% of sequence in fragments shorter than    2090 bp
  80% of sequence in fragments shorter than    2347 bp
  85% of sequence in fragments shorter than    2683 bp
  90% of sequence in fragments shorter than    3157 bp
  95% of sequence in fragments shorter than    4031 bp

# BsaAI.SfeI (YACGTR+CTRYAG) double digest, 4244 thousand fragments (713801+3531009 sites), 702 bp average fragment length
   5% of sequence in fragments shorter than     247 bp
  10% of sequence in fragments shorter than     372 bp
  15% of sequence in fragments shorter than     476 bp
  20% of sequence in fragments shorter than     576 bp
  25% of sequence in fragments shorter than     675 bp
  30% of sequence in fragments shorter than     773 bp
  35% of sequence in fragments shorter than     875 bp
  40% of sequence in fragments shorter than     977 bp
  45% of sequence in fragments shorter than    1084 bp
  50% of sequence in fragments shorter than    1200 bp
  55% of sequence in fragments shorter than    1324 bp
  60% of sequence in fragments shorter than    1456 bp
  65% of sequence in fragments shorter than    1607 bp
  70% of sequence in fragments shorter than    1773 bp
  75% of sequence in fragments shorter than    1969 bp
  80% of sequence in fragments shorter than    2184 bp
  85% of sequence in fragments shorter than    2480 bp
  90% of sequence in fragments shorter than    2890 bp
  95% of sequence in fragments shorter than    3602 bp

# AciI.SalI (CCGC+GTCGAC) double digest, 4237 thousand fragments (4206130+31396 sites), 703 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     427 bp
  15% of sequence in fragments shorter than     622 bp
  20% of sequence in fragments shorter than     818 bp
  25% of sequence in fragments shorter than    1011 bp
  30% of sequence in fragments shorter than    1213 bp
  35% of sequence in fragments shorter than    1427 bp
  40% of sequence in fragments shorter than    1660 bp
  45% of sequence in fragments shorter than    1915 bp
  50% of sequence in fragments shorter than    2196 bp
  55% of sequence in fragments shorter than    2504 bp
  60% of sequence in fragments shorter than    2849 bp
  65% of sequence in fragments shorter than    3244 bp
  70% of sequence in fragments shorter than    3704 bp
  75% of sequence in fragments shorter than    4246 bp
  80% of sequence in fragments shorter than    4913 bp
  85% of sequence in fragments shorter than    5772 bp
  90% of sequence in fragments shorter than    6984 bp
  95% of sequence in fragments shorter than    9108 bp

# BceAI.SfeI (ACGGC+CTRYAG) double digest, 4235 thousand fragments (705178+3531009 sites), 703 bp average fragment length
   5% of sequence in fragments shorter than     243 bp
  10% of sequence in fragments shorter than     369 bp
  15% of sequence in fragments shorter than     479 bp
  20% of sequence in fragments shorter than     584 bp
  25% of sequence in fragments shorter than     686 bp
  30% of sequence in fragments shorter than     790 bp
  35% of sequence in fragments shorter than     898 bp
  40% of sequence in fragments shorter than    1009 bp
  45% of sequence in fragments shorter than    1120 bp
  50% of sequence in fragments shorter than    1244 bp
  55% of sequence in fragments shorter than    1377 bp
  60% of sequence in fragments shorter than    1516 bp
  65% of sequence in fragments shorter than    1676 bp
  70% of sequence in fragments shorter than    1856 bp
  75% of sequence in fragments shorter than    2064 bp
  80% of sequence in fragments shorter than    2292 bp
  85% of sequence in fragments shorter than    2610 bp
  90% of sequence in fragments shorter than    3060 bp
  95% of sequence in fragments shorter than    3844 bp

# AflIII.AvaII (ACRYGT+GGWCC) double digest, 4232 thousand fragments (1485394+2747690 sites), 704 bp average fragment length
   5% of sequence in fragments shorter than     236 bp
  10% of sequence in fragments shorter than     366 bp
  15% of sequence in fragments shorter than     486 bp
  20% of sequence in fragments shorter than     601 bp
  25% of sequence in fragments shorter than     713 bp
  30% of sequence in fragments shorter than     829 bp
  35% of sequence in fragments shorter than     938 bp
  40% of sequence in fragments shorter than    1059 bp
  45% of sequence in fragments shorter than    1189 bp
  50% of sequence in fragments shorter than    1319 bp
  55% of sequence in fragments shorter than    1465 bp
  60% of sequence in fragments shorter than    1627 bp
  65% of sequence in fragments shorter than    1799 bp
  70% of sequence in fragments shorter than    2001 bp
  75% of sequence in fragments shorter than    2240 bp
  80% of sequence in fragments shorter than    2529 bp
  85% of sequence in fragments shorter than    2889 bp
  90% of sequence in fragments shorter than    3413 bp
  95% of sequence in fragments shorter than    4350 bp

# MlyI.SexAI (GAGTC+ACCWGGT) double digest, 4229 thousand fragments (3931692+297900 sites), 704 bp average fragment length
   5% of sequence in fragments shorter than     243 bp
  10% of sequence in fragments shorter than     363 bp
  15% of sequence in fragments shorter than     471 bp
  20% of sequence in fragments shorter than     574 bp
  25% of sequence in fragments shorter than     675 bp
  30% of sequence in fragments shorter than     776 bp
  35% of sequence in fragments shorter than     879 bp
  40% of sequence in fragments shorter than     988 bp
  45% of sequence in fragments shorter than    1101 bp
  50% of sequence in fragments shorter than    1225 bp
  55% of sequence in fragments shorter than    1359 bp
  60% of sequence in fragments shorter than    1507 bp
  65% of sequence in fragments shorter than    1673 bp
  70% of sequence in fragments shorter than    1859 bp
  75% of sequence in fragments shorter than    2083 bp
  80% of sequence in fragments shorter than    2366 bp
  85% of sequence in fragments shorter than    2744 bp
  90% of sequence in fragments shorter than    3279 bp
  95% of sequence in fragments shorter than    4285 bp

# AciI.MluI (CCGC+ACGCGT) double digest, 4227 thousand fragments (4206130+21415 sites), 704 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     428 bp
  15% of sequence in fragments shorter than     625 bp
  20% of sequence in fragments shorter than     822 bp
  25% of sequence in fragments shorter than    1017 bp
  30% of sequence in fragments shorter than    1220 bp
  35% of sequence in fragments shorter than    1437 bp
  40% of sequence in fragments shorter than    1672 bp
  45% of sequence in fragments shorter than    1929 bp
  50% of sequence in fragments shorter than    2212 bp
  55% of sequence in fragments shorter than    2524 bp
  60% of sequence in fragments shorter than    2873 bp
  65% of sequence in fragments shorter than    3272 bp
  70% of sequence in fragments shorter than    3737 bp
  75% of sequence in fragments shorter than    4286 bp
  80% of sequence in fragments shorter than    4960 bp
  85% of sequence in fragments shorter than    5834 bp
  90% of sequence in fragments shorter than    7060 bp
  95% of sequence in fragments shorter than    9211 bp

# BciVI.SfeI (GTATCC+CTRYAG) double digest, 4226 thousand fragments (696093+3531009 sites), 705 bp average fragment length
   5% of sequence in fragments shorter than     249 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     476 bp
  20% of sequence in fragments shorter than     577 bp
  25% of sequence in fragments shorter than     675 bp
  30% of sequence in fragments shorter than     774 bp
  35% of sequence in fragments shorter than     877 bp
  40% of sequence in fragments shorter than     980 bp
  45% of sequence in fragments shorter than    1087 bp
  50% of sequence in fragments shorter than    1203 bp
  55% of sequence in fragments shorter than    1328 bp
  60% of sequence in fragments shorter than    1457 bp
  65% of sequence in fragments shorter than    1605 bp
  70% of sequence in fragments shorter than    1759 bp
  75% of sequence in fragments shorter than    1949 bp
  80% of sequence in fragments shorter than    2179 bp
  85% of sequence in fragments shorter than    2472 bp
  90% of sequence in fragments shorter than    2884 bp
  95% of sequence in fragments shorter than    3603 bp

# BscAI.HgaI (GCATC+GACGC) double digest, 4224 thousand fragments (3652926+571889 sites), 705 bp average fragment length
   5% of sequence in fragments shorter than     237 bp
  10% of sequence in fragments shorter than     369 bp
  15% of sequence in fragments shorter than     488 bp
  20% of sequence in fragments shorter than     598 bp
  25% of sequence in fragments shorter than     704 bp
  30% of sequence in fragments shorter than     812 bp
  35% of sequence in fragments shorter than     924 bp
  40% of sequence in fragments shorter than    1042 bp
  45% of sequence in fragments shorter than    1165 bp
  50% of sequence in fragments shorter than    1290 bp
  55% of sequence in fragments shorter than    1428 bp
  60% of sequence in fragments shorter than    1581 bp
  65% of sequence in fragments shorter than    1747 bp
  70% of sequence in fragments shorter than    1939 bp
  75% of sequence in fragments shorter than    2161 bp
  80% of sequence in fragments shorter than    2432 bp
  85% of sequence in fragments shorter than    2781 bp
  90% of sequence in fragments shorter than    3273 bp
  95% of sequence in fragments shorter than    4176 bp

# BscAI.EciI (GCATC+GGCGGA) double digest, 4223 thousand fragments (3652926+571273 sites), 705 bp average fragment length
   5% of sequence in fragments shorter than     238 bp
  10% of sequence in fragments shorter than     367 bp
  15% of sequence in fragments shorter than     482 bp
  20% of sequence in fragments shorter than     589 bp
  25% of sequence in fragments shorter than     689 bp
  30% of sequence in fragments shorter than     791 bp
  35% of sequence in fragments shorter than     898 bp
  40% of sequence in fragments shorter than    1010 bp
  45% of sequence in fragments shorter than    1128 bp
  50% of sequence in fragments shorter than    1248 bp
  55% of sequence in fragments shorter than    1380 bp
  60% of sequence in fragments shorter than    1523 bp
  65% of sequence in fragments shorter than    1674 bp
  70% of sequence in fragments shorter than    1856 bp
  75% of sequence in fragments shorter than    2065 bp
  80% of sequence in fragments shorter than    2320 bp
  85% of sequence in fragments shorter than    2650 bp
  90% of sequence in fragments shorter than    3113 bp
  95% of sequence in fragments shorter than    3968 bp

# AciI.SgrAI (CCGC+CRCCGGYG) double digest, 4220 thousand fragments (4206130+15270 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     627 bp
  20% of sequence in fragments shorter than     825 bp
  25% of sequence in fragments shorter than    1021 bp
  30% of sequence in fragments shorter than    1225 bp
  35% of sequence in fragments shorter than    1442 bp
  40% of sequence in fragments shorter than    1677 bp
  45% of sequence in fragments shorter than    1935 bp
  50% of sequence in fragments shorter than    2220 bp
  55% of sequence in fragments shorter than    2533 bp
  60% of sequence in fragments shorter than    2883 bp
  65% of sequence in fragments shorter than    3283 bp
  70% of sequence in fragments shorter than    3750 bp
  75% of sequence in fragments shorter than    4301 bp
  80% of sequence in fragments shorter than    4977 bp
  85% of sequence in fragments shorter than    5855 bp
  90% of sequence in fragments shorter than    7086 bp
  95% of sequence in fragments shorter than    9250 bp

# AciI.NruI (CCGC+TCGCGA) double digest, 4220 thousand fragments (4206130+14872 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     627 bp
  20% of sequence in fragments shorter than     824 bp
  25% of sequence in fragments shorter than    1019 bp
  30% of sequence in fragments shorter than    1223 bp
  35% of sequence in fragments shorter than    1440 bp
  40% of sequence in fragments shorter than    1675 bp
  45% of sequence in fragments shorter than    1933 bp
  50% of sequence in fragments shorter than    2217 bp
  55% of sequence in fragments shorter than    2530 bp
  60% of sequence in fragments shorter than    2880 bp
  65% of sequence in fragments shorter than    3279 bp
  70% of sequence in fragments shorter than    3747 bp
  75% of sequence in fragments shorter than    4297 bp
  80% of sequence in fragments shorter than    4972 bp
  85% of sequence in fragments shorter than    5848 bp
  90% of sequence in fragments shorter than    7076 bp
  95% of sequence in fragments shorter than    9237 bp

# AciI.PvuI (CCGC+CGATCG) double digest, 4219 thousand fragments (4206130+14186 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     626 bp
  20% of sequence in fragments shorter than     824 bp
  25% of sequence in fragments shorter than    1019 bp
  30% of sequence in fragments shorter than    1223 bp
  35% of sequence in fragments shorter than    1439 bp
  40% of sequence in fragments shorter than    1675 bp
  45% of sequence in fragments shorter than    1932 bp
  50% of sequence in fragments shorter than    2216 bp
  55% of sequence in fragments shorter than    2529 bp
  60% of sequence in fragments shorter than    2878 bp
  65% of sequence in fragments shorter than    3278 bp
  70% of sequence in fragments shorter than    3744 bp
  75% of sequence in fragments shorter than    4294 bp
  80% of sequence in fragments shorter than    4969 bp
  85% of sequence in fragments shorter than    5844 bp
  90% of sequence in fragments shorter than    7074 bp
  95% of sequence in fragments shorter than    9231 bp

# AciI.FseI (CCGC+GGCCGGCC) double digest, 4219 thousand fragments (4206130+13662 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     627 bp
  20% of sequence in fragments shorter than     825 bp
  25% of sequence in fragments shorter than    1021 bp
  30% of sequence in fragments shorter than    1225 bp
  35% of sequence in fragments shorter than    1442 bp
  40% of sequence in fragments shorter than    1678 bp
  45% of sequence in fragments shorter than    1936 bp
  50% of sequence in fragments shorter than    2221 bp
  55% of sequence in fragments shorter than    2535 bp
  60% of sequence in fragments shorter than    2885 bp
  65% of sequence in fragments shorter than    3285 bp
  70% of sequence in fragments shorter than    3754 bp
  75% of sequence in fragments shorter than    4305 bp
  80% of sequence in fragments shorter than    4982 bp
  85% of sequence in fragments shorter than    5859 bp
  90% of sequence in fragments shorter than    7092 bp
  95% of sequence in fragments shorter than    9259 bp

# KpnI.MlyI (GGTACC+GAGTC) double digest, 4219 thousand fragments (287974+3931692 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     245 bp
  10% of sequence in fragments shorter than     366 bp
  15% of sequence in fragments shorter than     476 bp
  20% of sequence in fragments shorter than     579 bp
  25% of sequence in fragments shorter than     680 bp
  30% of sequence in fragments shorter than     781 bp
  35% of sequence in fragments shorter than     884 bp
  40% of sequence in fragments shorter than     992 bp
  45% of sequence in fragments shorter than    1102 bp
  50% of sequence in fragments shorter than    1221 bp
  55% of sequence in fragments shorter than    1347 bp
  60% of sequence in fragments shorter than    1488 bp
  65% of sequence in fragments shorter than    1645 bp
  70% of sequence in fragments shorter than    1824 bp
  75% of sequence in fragments shorter than    2036 bp
  80% of sequence in fragments shorter than    2297 bp
  85% of sequence in fragments shorter than    2642 bp
  90% of sequence in fragments shorter than    3134 bp
  95% of sequence in fragments shorter than    4088 bp

# MlyI.NheI (GAGTC+GCTAGC) double digest, 4218 thousand fragments (3931692+287384 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     247 bp
  10% of sequence in fragments shorter than     367 bp
  15% of sequence in fragments shorter than     476 bp
  20% of sequence in fragments shorter than     580 bp
  25% of sequence in fragments shorter than     678 bp
  30% of sequence in fragments shorter than     778 bp
  35% of sequence in fragments shorter than     881 bp
  40% of sequence in fragments shorter than     989 bp
  45% of sequence in fragments shorter than    1104 bp
  50% of sequence in fragments shorter than    1226 bp
  55% of sequence in fragments shorter than    1355 bp
  60% of sequence in fragments shorter than    1491 bp
  65% of sequence in fragments shorter than    1648 bp
  70% of sequence in fragments shorter than    1817 bp
  75% of sequence in fragments shorter than    2020 bp
  80% of sequence in fragments shorter than    2273 bp
  85% of sequence in fragments shorter than    2607 bp
  90% of sequence in fragments shorter than    3085 bp
  95% of sequence in fragments shorter than    3975 bp

# BscAI.MfeI (GCATC+CAATTG) double digest, 4216 thousand fragments (3652926+564303 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     367 bp
  15% of sequence in fragments shorter than     484 bp
  20% of sequence in fragments shorter than     593 bp
  25% of sequence in fragments shorter than     698 bp
  30% of sequence in fragments shorter than     803 bp
  35% of sequence in fragments shorter than     912 bp
  40% of sequence in fragments shorter than    1025 bp
  45% of sequence in fragments shorter than    1141 bp
  50% of sequence in fragments shorter than    1261 bp
  55% of sequence in fragments shorter than    1390 bp
  60% of sequence in fragments shorter than    1535 bp
  65% of sequence in fragments shorter than    1687 bp
  70% of sequence in fragments shorter than    1867 bp
  75% of sequence in fragments shorter than    2069 bp
  80% of sequence in fragments shorter than    2319 bp
  85% of sequence in fragments shorter than    2640 bp
  90% of sequence in fragments shorter than    3094 bp
  95% of sequence in fragments shorter than    3900 bp

# AciI.NotI (CCGC+GCGGCCGC) double digest, 4215 thousand fragments (4206130+9795 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     221 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     629 bp
  20% of sequence in fragments shorter than     827 bp
  25% of sequence in fragments shorter than    1023 bp
  30% of sequence in fragments shorter than    1227 bp
  35% of sequence in fragments shorter than    1444 bp
  40% of sequence in fragments shorter than    1680 bp
  45% of sequence in fragments shorter than    1938 bp
  50% of sequence in fragments shorter than    2224 bp
  55% of sequence in fragments shorter than    2538 bp
  60% of sequence in fragments shorter than    2888 bp
  65% of sequence in fragments shorter than    3289 bp
  70% of sequence in fragments shorter than    3758 bp
  75% of sequence in fragments shorter than    4309 bp
  80% of sequence in fragments shorter than    4987 bp
  85% of sequence in fragments shorter than    5864 bp
  90% of sequence in fragments shorter than    7098 bp
  95% of sequence in fragments shorter than    9264 bp

# AciI.RsrII (CCGC+CGGWCCG) double digest, 4215 thousand fragments (4206130+9764 sites), 706 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     628 bp
  20% of sequence in fragments shorter than     826 bp
  25% of sequence in fragments shorter than    1022 bp
  30% of sequence in fragments shorter than    1226 bp
  35% of sequence in fragments shorter than    1443 bp
  40% of sequence in fragments shorter than    1679 bp
  45% of sequence in fragments shorter than    1937 bp
  50% of sequence in fragments shorter than    2222 bp
  55% of sequence in fragments shorter than    2536 bp
  60% of sequence in fragments shorter than    2886 bp
  65% of sequence in fragments shorter than    3287 bp
  70% of sequence in fragments shorter than    3755 bp
  75% of sequence in fragments shorter than    4307 bp
  80% of sequence in fragments shorter than    4984 bp
  85% of sequence in fragments shorter than    5861 bp
  90% of sequence in fragments shorter than    7094 bp
  95% of sequence in fragments shorter than    9260 bp

# AccI.StyI (GTMKAC+CCWWGG) double digest, 4214 thousand fragments (1101063+3114107 sites), 707 bp average fragment length
   5% of sequence in fragments shorter than     245 bp
  10% of sequence in fragments shorter than     368 bp
  15% of sequence in fragments shorter than     481 bp
  20% of sequence in fragments shorter than     584 bp
  25% of sequence in fragments shorter than     687 bp
  30% of sequence in fragments shorter than     783 bp
  35% of sequence in fragments shorter than     889 bp
  40% of sequence in fragments shorter than    1000 bp
  45% of sequence in fragments shorter than    1114 bp
  50% of sequence in fragments shorter than    1235 bp
  55% of sequence in fragments shorter than    1366 bp
  60% of sequence in fragments shorter than    1509 bp
  65% of sequence in fragments shorter than    1671 bp
  70% of sequence in fragments shorter than    1852 bp
  75% of sequence in fragments shorter than    2066 bp
  80% of sequence in fragments shorter than    2327 bp
  85% of sequence in fragments shorter than    2663 bp
  90% of sequence in fragments shorter than    3130 bp
  95% of sequence in fragments shorter than    3937 bp

# AflIII.SmlI (ACRYGT+CTYRAG) double digest, 4212 thousand fragments (1485394+2727867 sites), 707 bp average fragment length
   5% of sequence in fragments shorter than     243 bp
  10% of sequence in fragments shorter than     375 bp
  15% of sequence in fragments shorter than     484 bp
  20% of sequence in fragments shorter than     589 bp
  25% of sequence in fragments shorter than     689 bp
  30% of sequence in fragments shorter than     789 bp
  35% of sequence in fragments shorter than     893 bp
  40% of sequence in fragments shorter than     999 bp
  45% of sequence in fragments shorter than    1109 bp
  50% of sequence in fragments shorter than    1226 bp
  55% of sequence in fragments shorter than    1349 bp
  60% of sequence in fragments shorter than    1483 bp
  65% of sequence in fragments shorter than    1637 bp
  70% of sequence in fragments shorter than    1809 bp
  75% of sequence in fragments shorter than    2007 bp
  80% of sequence in fragments shorter than    2240 bp
  85% of sequence in fragments shorter than    2549 bp
  90% of sequence in fragments shorter than    2935 bp
  95% of sequence in fragments shorter than    3683 bp

# AciI.AscI (CCGC+GGCGCGCC) double digest, 4210 thousand fragments (4206130+4751 sites), 707 bp average fragment length
   5% of sequence in fragments shorter than     220 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     629 bp
  20% of sequence in fragments shorter than     827 bp
  25% of sequence in fragments shorter than    1022 bp
  30% of sequence in fragments shorter than    1227 bp
  35% of sequence in fragments shorter than    1444 bp
  40% of sequence in fragments shorter than    1680 bp
  45% of sequence in fragments shorter than    1938 bp
  50% of sequence in fragments shorter than    2224 bp
  55% of sequence in fragments shorter than    2537 bp
  60% of sequence in fragments shorter than    2888 bp
  65% of sequence in fragments shorter than    3288 bp
  70% of sequence in fragments shorter than    3757 bp
  75% of sequence in fragments shorter than    4308 bp
  80% of sequence in fragments shorter than    4986 bp
  85% of sequence in fragments shorter than    5863 bp
  90% of sequence in fragments shorter than    7097 bp
  95% of sequence in fragments shorter than    9263 bp

# AccI.NspI (GTMKAC+RCATGY) double digest, 4204 thousand fragments (1101063+3104359 sites), 708 bp average fragment length
   5% of sequence in fragments shorter than     248 bp
  10% of sequence in fragments shorter than     385 bp
  15% of sequence in fragments shorter than     500 bp
  20% of sequence in fragments shorter than     610 bp
  25% of sequence in fragments shorter than     716 bp
  30% of sequence in fragments shorter than     823 bp
  35% of sequence in fragments shorter than     932 bp
  40% of sequence in fragments shorter than    1045 bp
  45% of sequence in fragments shorter than    1162 bp
  50% of sequence in fragments shorter than    1285 bp
  55% of sequence in fragments shorter than    1418 bp
  60% of sequence in fragments shorter than    1562 bp
  65% of sequence in fragments shorter than    1725 bp
  70% of sequence in fragments shorter than    1908 bp
  75% of sequence in fragments shorter than    2116 bp
  80% of sequence in fragments shorter than    2376 bp
  85% of sequence in fragments shorter than    2717 bp
  90% of sequence in fragments shorter than    3196 bp
  95% of sequence in fragments shorter than    4054 bp

# BscAI.SphI (GCATC+GCATGC) double digest, 4203 thousand fragments (3652926+550952 sites), 708 bp average fragment length
   5% of sequence in fragments shorter than     243 bp
  10% of sequence in fragments shorter than     373 bp
  15% of sequence in fragments shorter than     491 bp
  20% of sequence in fragments shorter than     598 bp
  25% of sequence in fragments shorter than     701 bp
  30% of sequence in fragments shorter than     805 bp
  35% of sequence in fragments shorter than     913 bp
  40% of sequence in fragments shorter than    1028 bp
  45% of sequence in fragments shorter than    1146 bp
  50% of sequence in fragments shorter than    1268 bp
  55% of sequence in fragments shorter than    1398 bp
  60% of sequence in fragments shorter than    1544 bp
  65% of sequence in fragments shorter than    1697 bp
  70% of sequence in fragments shorter than    1880 bp
  75% of sequence in fragments shorter than    2089 bp
  80% of sequence in fragments shorter than    2345 bp
  85% of sequence in fragments shorter than    2676 bp
  90% of sequence in fragments shorter than    3145 bp
  95% of sequence in fragments shorter than    4013 bp

# PpuMI.StyI (RGGWCCY+CCWWGG) double digest, 4198 thousand fragments (1085138+3114107 sites), 709 bp average fragment length
   5% of sequence in fragments shorter than     237 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     490 bp
  20% of sequence in fragments shorter than     604 bp
  25% of sequence in fragments shorter than     718 bp
  30% of sequence in fragments shorter than     829 bp
  35% of sequence in fragments shorter than     952 bp
  40% of sequence in fragments shorter than    1080 bp
  45% of sequence in fragments shorter than    1218 bp
  50% of sequence in fragments shorter than    1364 bp
  55% of sequence in fragments shorter than    1521 bp
  60% of sequence in fragments shorter than    1700 bp
  65% of sequence in fragments shorter than    1899 bp
  70% of sequence in fragments shorter than    2131 bp
  75% of sequence in fragments shorter than    2404 bp
  80% of sequence in fragments shorter than    2738 bp
  85% of sequence in fragments shorter than    3144 bp
  90% of sequence in fragments shorter than    3751 bp
  95% of sequence in fragments shorter than    4983 bp

# PvuII.StyI (CAGCTG+CCWWGG) double digest, 4197 thousand fragments (1084260+3114107 sites), 709 bp average fragment length
   5% of sequence in fragments shorter than     235 bp
  10% of sequence in fragments shorter than     366 bp
  15% of sequence in fragments shorter than     482 bp
  20% of sequence in fragments shorter than     592 bp
  25% of sequence in fragments shorter than     702 bp
  30% of sequence in fragments shorter than     807 bp
  35% of sequence in fragments shorter than     923 bp
  40% of sequence in fragments shorter than    1046 bp
  45% of sequence in fragments shorter than    1176 bp
  50% of sequence in fragments shorter than    1316 bp
  55% of sequence in fragments shorter than    1462 bp
  60% of sequence in fragments shorter than    1631 bp
  65% of sequence in fragments shorter than    1819 bp
  70% of sequence in fragments shorter than    2038 bp
  75% of sequence in fragments shorter than    2293 bp
  80% of sequence in fragments shorter than    2606 bp
  85% of sequence in fragments shorter than    2995 bp
  90% of sequence in fragments shorter than    3548 bp
  95% of sequence in fragments shorter than    4658 bp

# BscAI.ScaI (GCATC+AGTACT) double digest, 4196 thousand fragments (3652926+543793 sites), 710 bp average fragment length
   5% of sequence in fragments shorter than     243 bp
  10% of sequence in fragments shorter than     374 bp
  15% of sequence in fragments shorter than     492 bp
  20% of sequence in fragments shorter than     598 bp
  25% of sequence in fragments shorter than     700 bp
  30% of sequence in fragments shorter than     804 bp
  35% of sequence in fragments shorter than     911 bp
  40% of sequence in fragments shorter than    1024 bp
  45% of sequence in fragments shorter than    1140 bp
  50% of sequence in fragments shorter than    1260 bp
  55% of sequence in fragments shorter than    1388 bp
  60% of sequence in fragments shorter than    1533 bp
  65% of sequence in fragments shorter than    1682 bp
  70% of sequence in fragments shorter than    1862 bp
  75% of sequence in fragments shorter than    2069 bp
  80% of sequence in fragments shorter than    2320 bp
  85% of sequence in fragments shorter than    2643 bp
  90% of sequence in fragments shorter than    3101 bp
  95% of sequence in fragments shorter than    3957 bp

# AclI.Tsp45I (AACGTT+GTSAC) double digest, 4193 thousand fragments (172706+4021761 sites), 710 bp average fragment length
   5% of sequence in fragments shorter than     249 bp
  10% of sequence in fragments shorter than     368 bp
  15% of sequence in fragments shorter than     480 bp
  20% of sequence in fragments shorter than     584 bp
  25% of sequence in fragments shorter than     687 bp
  30% of sequence in fragments shorter than     788 bp
  35% of sequence in fragments shorter than     891 bp
  40% of sequence in fragments shorter than    1002 bp
  45% of sequence in fragments shorter than    1114 bp
  50% of sequence in fragments shorter than    1234 bp
  55% of sequence in fragments shorter than    1367 bp
  60% of sequence in fragments shorter than    1512 bp
  65% of sequence in fragments shorter than    1676 bp
  70% of sequence in fragments shorter than    1860 bp
  75% of sequence in fragments shorter than    2074 bp
  80% of sequence in fragments shorter than    2339 bp
  85% of sequence in fragments shorter than    2674 bp
  90% of sequence in fragments shorter than    3167 bp
  95% of sequence in fragments shorter than    4055 bp

# NspI.PpuMI (RCATGY+RGGWCCY) double digest, 4189 thousand fragments (3104359+1085138 sites), 711 bp average fragment length
   5% of sequence in fragments shorter than     243 bp
  10% of sequence in fragments shorter than     376 bp
  15% of sequence in fragments shorter than     491 bp
  20% of sequence in fragments shorter than     600 bp
  25% of sequence in fragments shorter than     705 bp
  30% of sequence in fragments shorter than     812 bp
  35% of sequence in fragments shorter than     921 bp
  40% of sequence in fragments shorter than    1033 bp
  45% of sequence in fragments shorter than    1149 bp
  50% of sequence in fragments shorter than    1264 bp
  55% of sequence in fragments shorter than    1396 bp
  60% of sequence in fragments shorter than    1542 bp
  65% of sequence in fragments shorter than    1702 bp
  70% of sequence in fragments shorter than    1886 bp
  75% of sequence in fragments shorter than    2095 bp
  80% of sequence in fragments shorter than    2352 bp
  85% of sequence in fragments shorter than    2680 bp
  90% of sequence in fragments shorter than    3158 bp
  95% of sequence in fragments shorter than    3996 bp

# NspI.PvuII (RCATGY+CAGCTG) double digest, 4188 thousand fragments (3104359+1084260 sites), 711 bp average fragment length
   5% of sequence in fragments shorter than     245 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     490 bp
  20% of sequence in fragments shorter than     600 bp
  25% of sequence in fragments shorter than     706 bp
  30% of sequence in fragments shorter than     812 bp
  35% of sequence in fragments shorter than     918 bp
  40% of sequence in fragments shorter than    1030 bp
  45% of sequence in fragments shorter than    1144 bp
  50% of sequence in fragments shorter than    1267 bp
  55% of sequence in fragments shorter than    1397 bp
  60% of sequence in fragments shorter than    1541 bp
  65% of sequence in fragments shorter than    1703 bp
  70% of sequence in fragments shorter than    1887 bp
  75% of sequence in fragments shorter than    2097 bp
  80% of sequence in fragments shorter than    2353 bp
  85% of sequence in fragments shorter than    2689 bp
  90% of sequence in fragments shorter than    3162 bp
  95% of sequence in fragments shorter than    4012 bp

# AvaII.FauI (GGWCC+CCCGC) double digest, 4186 thousand fragments (2747690+1439772 sites), 711 bp average fragment length
   5% of sequence in fragments shorter than     227 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     517 bp
  20% of sequence in fragments shorter than     661 bp
  25% of sequence in fragments shorter than     802 bp
  30% of sequence in fragments shorter than     937 bp
  35% of sequence in fragments shorter than    1092 bp
  40% of sequence in fragments shorter than    1255 bp
  45% of sequence in fragments shorter than    1425 bp
  50% of sequence in fragments shorter than    1613 bp
  55% of sequence in fragments shorter than    1813 bp
  60% of sequence in fragments shorter than    2036 bp
  65% of sequence in fragments shorter than    2289 bp
  70% of sequence in fragments shorter than    2582 bp
  75% of sequence in fragments shorter than    2915 bp
  80% of sequence in fragments shorter than    3332 bp
  85% of sequence in fragments shorter than    3878 bp
  90% of sequence in fragments shorter than    4660 bp
  95% of sequence in fragments shorter than    6074 bp

# AvaII.BspMI (GGWCC+ACCTGC) double digest, 4185 thousand fragments (2747690+1438129 sites), 712 bp average fragment length
   5% of sequence in fragments shorter than     235 bp
  10% of sequence in fragments shorter than     360 bp
  15% of sequence in fragments shorter than     492 bp
  20% of sequence in fragments shorter than     616 bp
  25% of sequence in fragments shorter than     735 bp
  30% of sequence in fragments shorter than     864 bp
  35% of sequence in fragments shorter than     989 bp
  40% of sequence in fragments shorter than    1129 bp
  45% of sequence in fragments shorter than    1276 bp
  50% of sequence in fragments shorter than    1431 bp
  55% of sequence in fragments shorter than    1602 bp
  60% of sequence in fragments shorter than    1770 bp
  65% of sequence in fragments shorter than    1978 bp
  70% of sequence in fragments shorter than    2217 bp
  75% of sequence in fragments shorter than    2503 bp
  80% of sequence in fragments shorter than    2839 bp
  85% of sequence in fragments shorter than    3276 bp
  90% of sequence in fragments shorter than    3896 bp
  95% of sequence in fragments shorter than    4966 bp

# PacI.Tsp45I (TTAATTAA+GTSAC) double digest, 4180 thousand fragments (159455+4021761 sites), 712 bp average fragment length
   5% of sequence in fragments shorter than     250 bp
  10% of sequence in fragments shorter than     370 bp
  15% of sequence in fragments shorter than     482 bp
  20% of sequence in fragments shorter than     586 bp
  25% of sequence in fragments shorter than     689 bp
  30% of sequence in fragments shorter than     791 bp
  35% of sequence in fragments shorter than     894 bp
  40% of sequence in fragments shorter than    1004 bp
  45% of sequence in fragments shorter than    1120 bp
  50% of sequence in fragments shorter than    1241 bp
  55% of sequence in fragments shorter than    1375 bp
  60% of sequence in fragments shorter than    1524 bp
  65% of sequence in fragments shorter than    1687 bp
  70% of sequence in fragments shorter than    1875 bp
  75% of sequence in fragments shorter than    2095 bp
  80% of sequence in fragments shorter than    2368 bp
  85% of sequence in fragments shorter than    2729 bp
  90% of sequence in fragments shorter than    3254 bp
  95% of sequence in fragments shorter than    4207 bp

# Hpy99I.Tsp45I (CGWCG+GTSAC) double digest, 4179 thousand fragments (157917+4021761 sites), 713 bp average fragment length
   5% of sequence in fragments shorter than     248 bp
  10% of sequence in fragments shorter than     369 bp
  15% of sequence in fragments shorter than     481 bp
  20% of sequence in fragments shorter than     586 bp
  25% of sequence in fragments shorter than     691 bp
  30% of sequence in fragments shorter than     795 bp
  35% of sequence in fragments shorter than     900 bp
  40% of sequence in fragments shorter than    1013 bp
  45% of sequence in fragments shorter than    1131 bp
  50% of sequence in fragments shorter than    1256 bp
  55% of sequence in fragments shorter than    1392 bp
  60% of sequence in fragments shorter than    1545 bp
  65% of sequence in fragments shorter than    1714 bp
  70% of sequence in fragments shorter than    1907 bp
  75% of sequence in fragments shorter than    2136 bp
  80% of sequence in fragments shorter than    2417 bp
  85% of sequence in fragments shorter than    2791 bp
  90% of sequence in fragments shorter than    3337 bp
  95% of sequence in fragments shorter than    4311 bp

# AflII.SfeI (CTTAAG+CTRYAG) double digest, 4173 thousand fragments (643284+3531009 sites), 713 bp average fragment length
   5% of sequence in fragments shorter than     251 bp
  10% of sequence in fragments shorter than     377 bp
  15% of sequence in fragments shorter than     485 bp
  20% of sequence in fragments shorter than     584 bp
  25% of sequence in fragments shorter than     684 bp
  30% of sequence in fragments shorter than     785 bp
  35% of sequence in fragments shorter than     888 bp
  40% of sequence in fragments shorter than     994 bp
  45% of sequence in fragments shorter than    1102 bp
  50% of sequence in fragments shorter than    1217 bp
  55% of sequence in fragments shorter than    1344 bp
  60% of sequence in fragments shorter than    1475 bp
  65% of sequence in fragments shorter than    1623 bp
  70% of sequence in fragments shorter than    1778 bp
  75% of sequence in fragments shorter than    1969 bp
  80% of sequence in fragments shorter than    2199 bp
  85% of sequence in fragments shorter than    2495 bp
  90% of sequence in fragments shorter than    2908 bp
  95% of sequence in fragments shorter than    3640 bp

# FauI.SmlI (CCCGC+CTYRAG) double digest, 4167 thousand fragments (1439772+2727867 sites), 715 bp average fragment length
   5% of sequence in fragments shorter than     238 bp
  10% of sequence in fragments shorter than     380 bp
  15% of sequence in fragments shorter than     505 bp
  20% of sequence in fragments shorter than     622 bp
  25% of sequence in fragments shorter than     735 bp
  30% of sequence in fragments shorter than     855 bp
  35% of sequence in fragments shorter than     975 bp
  40% of sequence in fragments shorter than    1098 bp
  45% of sequence in fragments shorter than    1231 bp
  50% of sequence in fragments shorter than    1372 bp
  55% of sequence in fragments shorter than    1525 bp
  60% of sequence in fragments shorter than    1698 bp
  65% of sequence in fragments shorter than    1889 bp
  70% of sequence in fragments shorter than    2096 bp
  75% of sequence in fragments shorter than    2351 bp
  80% of sequence in fragments shorter than    2646 bp
  85% of sequence in fragments shorter than    3016 bp
  90% of sequence in fragments shorter than    3576 bp
  95% of sequence in fragments shorter than    4595 bp

# BsrBI.MlyI (CCGCTC+GAGTC) double digest, 4166 thousand fragments (235523+3931692 sites), 715 bp average fragment length
   5% of sequence in fragments shorter than     247 bp
  10% of sequence in fragments shorter than     368 bp
  15% of sequence in fragments shorter than     479 bp
  20% of sequence in fragments shorter than     585 bp
  25% of sequence in fragments shorter than     688 bp
  30% of sequence in fragments shorter than     791 bp
  35% of sequence in fragments shorter than     897 bp
  40% of sequence in fragments shorter than    1009 bp
  45% of sequence in fragments shorter than    1126 bp
  50% of sequence in fragments shorter than    1255 bp
  55% of sequence in fragments shorter than    1392 bp
  60% of sequence in fragments shorter than    1545 bp
  65% of sequence in fragments shorter than    1715 bp
  70% of sequence in fragments shorter than    1906 bp
  75% of sequence in fragments shorter than    2141 bp
  80% of sequence in fragments shorter than    2433 bp
  85% of sequence in fragments shorter than    2816 bp
  90% of sequence in fragments shorter than    3370 bp
  95% of sequence in fragments shorter than    4406 bp

# BspMI.SmlI (ACCTGC+CTYRAG) double digest, 4165 thousand fragments (1438129+2727867 sites), 715 bp average fragment length
   5% of sequence in fragments shorter than     244 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     485 bp
  20% of sequence in fragments shorter than     589 bp
  25% of sequence in fragments shorter than     691 bp
  30% of sequence in fragments shorter than     795 bp
  35% of sequence in fragments shorter than     898 bp
  40% of sequence in fragments shorter than    1008 bp
  45% of sequence in fragments shorter than    1123 bp
  50% of sequence in fragments shorter than    1239 bp
  55% of sequence in fragments shorter than    1370 bp
  60% of sequence in fragments shorter than    1500 bp
  65% of sequence in fragments shorter than    1657 bp
  70% of sequence in fragments shorter than    1836 bp
  75% of sequence in fragments shorter than    2032 bp
  80% of sequence in fragments shorter than    2279 bp
  85% of sequence in fragments shorter than    2600 bp
  90% of sequence in fragments shorter than    3034 bp
  95% of sequence in fragments shorter than    3792 bp

# KasI.MlyI (GGCGCC+GAGTC) double digest, 4161 thousand fragments (230381+3931692 sites), 716 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     368 bp
  15% of sequence in fragments shorter than     481 bp
  20% of sequence in fragments shorter than     589 bp
  25% of sequence in fragments shorter than     694 bp
  30% of sequence in fragments shorter than     799 bp
  35% of sequence in fragments shorter than     906 bp
  40% of sequence in fragments shorter than    1019 bp
  45% of sequence in fragments shorter than    1138 bp
  50% of sequence in fragments shorter than    1269 bp
  55% of sequence in fragments shorter than    1408 bp
  60% of sequence in fragments shorter than    1563 bp
  65% of sequence in fragments shorter than    1731 bp
  70% of sequence in fragments shorter than    1930 bp
  75% of sequence in fragments shorter than    2167 bp
  80% of sequence in fragments shorter than    2467 bp
  85% of sequence in fragments shorter than    2857 bp
  90% of sequence in fragments shorter than    3421 bp
  95% of sequence in fragments shorter than    4481 bp

# HaeII.SfeI (RGCGCY+CTRYAG) double digest, 4160 thousand fragments (630101+3531009 sites), 716 bp average fragment length
   5% of sequence in fragments shorter than     252 bp
  10% of sequence in fragments shorter than     376 bp
  15% of sequence in fragments shorter than     490 bp
  20% of sequence in fragments shorter than     600 bp
  25% of sequence in fragments shorter than     701 bp
  30% of sequence in fragments shorter than     812 bp
  35% of sequence in fragments shorter than     923 bp
  40% of sequence in fragments shorter than    1036 bp
  45% of sequence in fragments shorter than    1155 bp
  50% of sequence in fragments shorter than    1284 bp
  55% of sequence in fragments shorter than    1423 bp
  60% of sequence in fragments shorter than    1572 bp
  65% of sequence in fragments shorter than    1738 bp
  70% of sequence in fragments shorter than    1924 bp
  75% of sequence in fragments shorter than    2131 bp
  80% of sequence in fragments shorter than    2381 bp
  85% of sequence in fragments shorter than    2711 bp
  90% of sequence in fragments shorter than    3169 bp
  95% of sequence in fragments shorter than    3950 bp

# MlyI.SwaI (GAGTC+ATTTAAAT) double digest, 4157 thousand fragments (3931692+226602 sites), 716 bp average fragment length
   5% of sequence in fragments shorter than     251 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     481 bp
  20% of sequence in fragments shorter than     584 bp
  25% of sequence in fragments shorter than     685 bp
  30% of sequence in fragments shorter than     785 bp
  35% of sequence in fragments shorter than     887 bp
  40% of sequence in fragments shorter than     996 bp
  45% of sequence in fragments shorter than    1107 bp
  50% of sequence in fragments shorter than    1229 bp
  55% of sequence in fragments shorter than    1361 bp
  60% of sequence in fragments shorter than    1504 bp
  65% of sequence in fragments shorter than    1665 bp
  70% of sequence in fragments shorter than    1846 bp
  75% of sequence in fragments shorter than    2062 bp
  80% of sequence in fragments shorter than    2336 bp
  85% of sequence in fragments shorter than    2698 bp
  90% of sequence in fragments shorter than    3210 bp
  95% of sequence in fragments shorter than    4187 bp

# SnaBI.Tsp45I (TACGTA+GTSAC) double digest, 4153 thousand fragments (132299+4021761 sites), 717 bp average fragment length
   5% of sequence in fragments shorter than     251 bp
  10% of sequence in fragments shorter than     372 bp
  15% of sequence in fragments shorter than     485 bp
  20% of sequence in fragments shorter than     589 bp
  25% of sequence in fragments shorter than     692 bp
  30% of sequence in fragments shorter than     796 bp
  35% of sequence in fragments shorter than     900 bp
  40% of sequence in fragments shorter than    1011 bp
  45% of sequence in fragments shorter than    1128 bp
  50% of sequence in fragments shorter than    1251 bp
  55% of sequence in fragments shorter than    1384 bp
  60% of sequence in fragments shorter than    1536 bp
  65% of sequence in fragments shorter than    1700 bp
  70% of sequence in fragments shorter than    1889 bp
  75% of sequence in fragments shorter than    2112 bp
  80% of sequence in fragments shorter than    2387 bp
  85% of sequence in fragments shorter than    2750 bp
  90% of sequence in fragments shorter than    3279 bp
  95% of sequence in fragments shorter than    4226 bp

# ApaLI.BscAI (GTGCAC+GCATC) double digest, 4148 thousand fragments (496255+3652926 sites), 718 bp average fragment length
   5% of sequence in fragments shorter than     245 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     497 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     709 bp
  30% of sequence in fragments shorter than     814 bp
  35% of sequence in fragments shorter than     924 bp
  40% of sequence in fragments shorter than    1039 bp
  45% of sequence in fragments shorter than    1159 bp
  50% of sequence in fragments shorter than    1282 bp
  55% of sequence in fragments shorter than    1412 bp
  60% of sequence in fragments shorter than    1551 bp
  65% of sequence in fragments shorter than    1708 bp
  70% of sequence in fragments shorter than    1894 bp
  75% of sequence in fragments shorter than    2107 bp
  80% of sequence in fragments shorter than    2368 bp
  85% of sequence in fragments shorter than    2706 bp
  90% of sequence in fragments shorter than    3188 bp
  95% of sequence in fragments shorter than    4067 bp

# NaeI.Tsp45I (GCCGGC+GTSAC) double digest, 4146 thousand fragments (125692+4021761 sites), 718 bp average fragment length
   5% of sequence in fragments shorter than     250 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     485 bp
  20% of sequence in fragments shorter than     590 bp
  25% of sequence in fragments shorter than     694 bp
  30% of sequence in fragments shorter than     800 bp
  35% of sequence in fragments shorter than     906 bp
  40% of sequence in fragments shorter than    1019 bp
  45% of sequence in fragments shorter than    1138 bp
  50% of sequence in fragments shorter than    1264 bp
  55% of sequence in fragments shorter than    1401 bp
  60% of sequence in fragments shorter than    1556 bp
  65% of sequence in fragments shorter than    1724 bp
  70% of sequence in fragments shorter than    1919 bp
  75% of sequence in fragments shorter than    2151 bp
  80% of sequence in fragments shorter than    2436 bp
  85% of sequence in fragments shorter than    2812 bp
  90% of sequence in fragments shorter than    3367 bp
  95% of sequence in fragments shorter than    4353 bp

# Tsp45I.XhoI (GTSAC+CTCGAG) double digest, 4142 thousand fragments (4021761+121308 sites), 719 bp average fragment length
   5% of sequence in fragments shorter than     250 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     485 bp
  20% of sequence in fragments shorter than     591 bp
  25% of sequence in fragments shorter than     694 bp
  30% of sequence in fragments shorter than     799 bp
  35% of sequence in fragments shorter than     904 bp
  40% of sequence in fragments shorter than    1016 bp
  45% of sequence in fragments shorter than    1134 bp
  50% of sequence in fragments shorter than    1259 bp
  55% of sequence in fragments shorter than    1395 bp
  60% of sequence in fragments shorter than    1548 bp
  65% of sequence in fragments shorter than    1718 bp
  70% of sequence in fragments shorter than    1911 bp
  75% of sequence in fragments shorter than    2141 bp
  80% of sequence in fragments shorter than    2422 bp
  85% of sequence in fragments shorter than    2794 bp
  90% of sequence in fragments shorter than    3340 bp
  95% of sequence in fragments shorter than    4316 bp

# SacI.SfeI (GAGCTC+CTRYAG) double digest, 4133 thousand fragments (603323+3531009 sites), 720 bp average fragment length
   5% of sequence in fragments shorter than     251 bp
  10% of sequence in fragments shorter than     380 bp
  15% of sequence in fragments shorter than     490 bp
  20% of sequence in fragments shorter than     596 bp
  25% of sequence in fragments shorter than     694 bp
  30% of sequence in fragments shorter than     801 bp
  35% of sequence in fragments shorter than     909 bp
  40% of sequence in fragments shorter than    1020 bp
  45% of sequence in fragments shorter than    1134 bp
  50% of sequence in fragments shorter than    1258 bp
  55% of sequence in fragments shorter than    1385 bp
  60% of sequence in fragments shorter than    1529 bp
  65% of sequence in fragments shorter than    1688 bp
  70% of sequence in fragments shorter than    1870 bp
  75% of sequence in fragments shorter than    2075 bp
  80% of sequence in fragments shorter than    2304 bp
  85% of sequence in fragments shorter than    2619 bp
  90% of sequence in fragments shorter than    3065 bp
  95% of sequence in fragments shorter than    3851 bp

# HhaI.SspI (GCGC+AATATT) double digest, 4133 thousand fragments (1756502+2377267 sites), 720 bp average fragment length
   5% of sequence in fragments shorter than     245 bp
  10% of sequence in fragments shorter than     385 bp
  15% of sequence in fragments shorter than     517 bp
  20% of sequence in fragments shorter than     639 bp
  25% of sequence in fragments shorter than     751 bp
  30% of sequence in fragments shorter than     871 bp
  35% of sequence in fragments shorter than     998 bp
  40% of sequence in fragments shorter than    1124 bp
  45% of sequence in fragments shorter than    1257 bp
  50% of sequence in fragments shorter than    1401 bp
  55% of sequence in fragments shorter than    1558 bp
  60% of sequence in fragments shorter than    1729 bp
  65% of sequence in fragments shorter than    1919 bp
  70% of sequence in fragments shorter than    2137 bp
  75% of sequence in fragments shorter than    2394 bp
  80% of sequence in fragments shorter than    2702 bp
  85% of sequence in fragments shorter than    3097 bp
  90% of sequence in fragments shorter than    3656 bp
  95% of sequence in fragments shorter than    4551 bp

# AvrII.SfeI (CCTAGG+CTRYAG) double digest, 4125 thousand fragments (594956+3531009 sites), 722 bp average fragment length
   5% of sequence in fragments shorter than     251 bp
  10% of sequence in fragments shorter than     374 bp
  15% of sequence in fragments shorter than     490 bp
  20% of sequence in fragments shorter than     597 bp
  25% of sequence in fragments shorter than     698 bp
  30% of sequence in fragments shorter than     805 bp
  35% of sequence in fragments shorter than     914 bp
  40% of sequence in fragments shorter than    1025 bp
  45% of sequence in fragments shorter than    1136 bp
  50% of sequence in fragments shorter than    1259 bp
  55% of sequence in fragments shorter than    1385 bp
  60% of sequence in fragments shorter than    1528 bp
  65% of sequence in fragments shorter than    1686 bp
  70% of sequence in fragments shorter than    1848 bp
  75% of sequence in fragments shorter than    2051 bp
  80% of sequence in fragments shorter than    2293 bp
  85% of sequence in fragments shorter than    2608 bp
  90% of sequence in fragments shorter than    3049 bp
  95% of sequence in fragments shorter than    3830 bp

# AvaI.StyI (CYCGRG+CCWWGG) double digest, 4119 thousand fragments (1005714+3114107 sites), 723 bp average fragment length
   5% of sequence in fragments shorter than     235 bp
  10% of sequence in fragments shorter than     370 bp
  15% of sequence in fragments shorter than     489 bp
  20% of sequence in fragments shorter than     603 bp
  25% of sequence in fragments shorter than     717 bp
  30% of sequence in fragments shorter than     828 bp
  35% of sequence in fragments shorter than     953 bp
  40% of sequence in fragments shorter than    1082 bp
  45% of sequence in fragments shorter than    1221 bp
  50% of sequence in fragments shorter than    1369 bp
  55% of sequence in fragments shorter than    1527 bp
  60% of sequence in fragments shorter than    1710 bp
  65% of sequence in fragments shorter than    1914 bp
  70% of sequence in fragments shorter than    2153 bp
  75% of sequence in fragments shorter than    2434 bp
  80% of sequence in fragments shorter than    2775 bp
  85% of sequence in fragments shorter than    3192 bp
  90% of sequence in fragments shorter than    3838 bp
  95% of sequence in fragments shorter than    5133 bp

# ApaI.BscAI (GGGCCC+GCATC) double digest, 4112 thousand fragments (460339+3652926 sites), 724 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     501 bp
  20% of sequence in fragments shorter than     615 bp
  25% of sequence in fragments shorter than     724 bp
  30% of sequence in fragments shorter than     835 bp
  35% of sequence in fragments shorter than     953 bp
  40% of sequence in fragments shorter than    1076 bp
  45% of sequence in fragments shorter than    1202 bp
  50% of sequence in fragments shorter than    1336 bp
  55% of sequence in fragments shorter than    1479 bp
  60% of sequence in fragments shorter than    1631 bp
  65% of sequence in fragments shorter than    1806 bp
  70% of sequence in fragments shorter than    2005 bp
  75% of sequence in fragments shorter than    2239 bp
  80% of sequence in fragments shorter than    2523 bp
  85% of sequence in fragments shorter than    2887 bp
  90% of sequence in fragments shorter than    3415 bp
  95% of sequence in fragments shorter than    4379 bp

# AvaI.NspI (CYCGRG+RCATGY) double digest, 4109 thousand fragments (1005714+3104359 sites), 725 bp average fragment length
   5% of sequence in fragments shorter than     255 bp
  10% of sequence in fragments shorter than     389 bp
  15% of sequence in fragments shorter than     504 bp
  20% of sequence in fragments shorter than     616 bp
  25% of sequence in fragments shorter than     725 bp
  30% of sequence in fragments shorter than     834 bp
  35% of sequence in fragments shorter than     946 bp
  40% of sequence in fragments shorter than    1062 bp
  45% of sequence in fragments shorter than    1181 bp
  50% of sequence in fragments shorter than    1310 bp
  55% of sequence in fragments shorter than    1450 bp
  60% of sequence in fragments shorter than    1602 bp
  65% of sequence in fragments shorter than    1770 bp
  70% of sequence in fragments shorter than    1962 bp
  75% of sequence in fragments shorter than    2183 bp
  80% of sequence in fragments shorter than    2459 bp
  85% of sequence in fragments shorter than    2814 bp
  90% of sequence in fragments shorter than    3329 bp
  95% of sequence in fragments shorter than    4185 bp

# ClaI.Tsp45I (ATCGAT+GTSAC) double digest, 4107 thousand fragments (86477+4021761 sites), 725 bp average fragment length
   5% of sequence in fragments shorter than     253 bp
  10% of sequence in fragments shorter than     376 bp
  15% of sequence in fragments shorter than     490 bp
  20% of sequence in fragments shorter than     596 bp
  25% of sequence in fragments shorter than     699 bp
  30% of sequence in fragments shorter than     806 bp
  35% of sequence in fragments shorter than     911 bp
  40% of sequence in fragments shorter than    1024 bp
  45% of sequence in fragments shorter than    1141 bp
  50% of sequence in fragments shorter than    1266 bp
  55% of sequence in fragments shorter than    1401 bp
  60% of sequence in fragments shorter than    1554 bp
  65% of sequence in fragments shorter than    1719 bp
  70% of sequence in fragments shorter than    1908 bp
  75% of sequence in fragments shorter than    2132 bp
  80% of sequence in fragments shorter than    2407 bp
  85% of sequence in fragments shorter than    2767 bp
  90% of sequence in fragments shorter than    3285 bp
  95% of sequence in fragments shorter than    4208 bp

# AclI.MlyI (AACGTT+GAGTC) double digest, 4103 thousand fragments (172706+3931692 sites), 726 bp average fragment length
   5% of sequence in fragments shorter than     254 bp
  10% of sequence in fragments shorter than     376 bp
  15% of sequence in fragments shorter than     488 bp
  20% of sequence in fragments shorter than     594 bp
  25% of sequence in fragments shorter than     696 bp
  30% of sequence in fragments shorter than     799 bp
  35% of sequence in fragments shorter than     903 bp
  40% of sequence in fragments shorter than    1013 bp
  45% of sequence in fragments shorter than    1126 bp
  50% of sequence in fragments shorter than    1250 bp
  55% of sequence in fragments shorter than    1382 bp
  60% of sequence in fragments shorter than    1526 bp
  65% of sequence in fragments shorter than    1690 bp
  70% of sequence in fragments shorter than    1875 bp
  75% of sequence in fragments shorter than    2093 bp
  80% of sequence in fragments shorter than    2369 bp
  85% of sequence in fragments shorter than    2732 bp
  90% of sequence in fragments shorter than    3253 bp
  95% of sequence in fragments shorter than    4210 bp

# HgaI.SfeI (GACGC+CTRYAG) double digest, 4102 thousand fragments (571889+3531009 sites), 726 bp average fragment length
   5% of sequence in fragments shorter than     252 bp
  10% of sequence in fragments shorter than     382 bp
  15% of sequence in fragments shorter than     494 bp
  20% of sequence in fragments shorter than     604 bp
  25% of sequence in fragments shorter than     707 bp
  30% of sequence in fragments shorter than     817 bp
  35% of sequence in fragments shorter than     927 bp
  40% of sequence in fragments shorter than    1039 bp
  45% of sequence in fragments shorter than    1155 bp
  50% of sequence in fragments shorter than    1282 bp
  55% of sequence in fragments shorter than    1415 bp
  60% of sequence in fragments shorter than    1561 bp
  65% of sequence in fragments shorter than    1724 bp
  70% of sequence in fragments shorter than    1903 bp
  75% of sequence in fragments shorter than    2114 bp
  80% of sequence in fragments shorter than    2360 bp
  85% of sequence in fragments shorter than    2689 bp
  90% of sequence in fragments shorter than    3145 bp
  95% of sequence in fragments shorter than    3957 bp

# EciI.SfeI (GGCGGA+CTRYAG) double digest, 4101 thousand fragments (571273+3531009 sites), 726 bp average fragment length
   5% of sequence in fragments shorter than     250 bp
  10% of sequence in fragments shorter than     384 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     608 bp
  25% of sequence in fragments shorter than     713 bp
  30% of sequence in fragments shorter than     825 bp
  35% of sequence in fragments shorter than     937 bp
  40% of sequence in fragments shorter than    1050 bp
  45% of sequence in fragments shorter than    1168 bp
  50% of sequence in fragments shorter than    1297 bp
  55% of sequence in fragments shorter than    1430 bp
  60% of sequence in fragments shorter than    1582 bp
  65% of sequence in fragments shorter than    1751 bp
  70% of sequence in fragments shorter than    1935 bp
  75% of sequence in fragments shorter than    2135 bp
  80% of sequence in fragments shorter than    2397 bp
  85% of sequence in fragments shorter than    2734 bp
  90% of sequence in fragments shorter than    3207 bp
  95% of sequence in fragments shorter than    4046 bp

# BscAI.EcoRV (GCATC+GATATC) double digest, 4098 thousand fragments (3652926+446473 sites), 727 bp average fragment length
   5% of sequence in fragments shorter than     249 bp
  10% of sequence in fragments shorter than     383 bp
  15% of sequence in fragments shorter than     504 bp
  20% of sequence in fragments shorter than     612 bp
  25% of sequence in fragments shorter than     715 bp
  30% of sequence in fragments shorter than     819 bp
  35% of sequence in fragments shorter than     930 bp
  40% of sequence in fragments shorter than    1046 bp
  45% of sequence in fragments shorter than    1165 bp
  50% of sequence in fragments shorter than    1288 bp
  55% of sequence in fragments shorter than    1421 bp
  60% of sequence in fragments shorter than    1570 bp
  65% of sequence in fragments shorter than    1733 bp
  70% of sequence in fragments shorter than    1919 bp
  75% of sequence in fragments shorter than    2132 bp
  80% of sequence in fragments shorter than    2394 bp
  85% of sequence in fragments shorter than    2726 bp
  90% of sequence in fragments shorter than    3202 bp
  95% of sequence in fragments shorter than    4072 bp

# MfeI.SfeI (CAATTG+CTRYAG) double digest, 4094 thousand fragments (564303+3531009 sites), 727 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     381 bp
  15% of sequence in fragments shorter than     492 bp
  20% of sequence in fragments shorter than     597 bp
  25% of sequence in fragments shorter than     695 bp
  30% of sequence in fragments shorter than     800 bp
  35% of sequence in fragments shorter than     905 bp
  40% of sequence in fragments shorter than    1013 bp
  45% of sequence in fragments shorter than    1119 bp
  50% of sequence in fragments shorter than    1238 bp
  55% of sequence in fragments shorter than    1366 bp
  60% of sequence in fragments shorter than    1498 bp
  65% of sequence in fragments shorter than    1649 bp
  70% of sequence in fragments shorter than    1803 bp
  75% of sequence in fragments shorter than    1993 bp
  80% of sequence in fragments shorter than    2221 bp
  85% of sequence in fragments shorter than    2516 bp
  90% of sequence in fragments shorter than    2920 bp
  95% of sequence in fragments shorter than    3624 bp

# AatII.Tsp45I (GACGTC+GTSAC) double digest, 4092 thousand fragments (71089+4021761 sites), 728 bp average fragment length
   5% of sequence in fragments shorter than     254 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     493 bp
  20% of sequence in fragments shorter than     599 bp
  25% of sequence in fragments shorter than     703 bp
  30% of sequence in fragments shorter than     811 bp
  35% of sequence in fragments shorter than     917 bp
  40% of sequence in fragments shorter than    1031 bp
  45% of sequence in fragments shorter than    1150 bp
  50% of sequence in fragments shorter than    1277 bp
  55% of sequence in fragments shorter than    1415 bp
  60% of sequence in fragments shorter than    1570 bp
  65% of sequence in fragments shorter than    1738 bp
  70% of sequence in fragments shorter than    1933 bp
  75% of sequence in fragments shorter than    2165 bp
  80% of sequence in fragments shorter than    2449 bp
  85% of sequence in fragments shorter than    2823 bp
  90% of sequence in fragments shorter than    3374 bp
  95% of sequence in fragments shorter than    4348 bp

# BspHI.StyI (TCATGA+CCWWGG) double digest, 4091 thousand fragments (978290+3114107 sites), 728 bp average fragment length
   5% of sequence in fragments shorter than     253 bp
  10% of sequence in fragments shorter than     384 bp
  15% of sequence in fragments shorter than     495 bp
  20% of sequence in fragments shorter than     603 bp
  25% of sequence in fragments shorter than     707 bp
  30% of sequence in fragments shorter than     806 bp
  35% of sequence in fragments shorter than     916 bp
  40% of sequence in fragments shorter than    1029 bp
  45% of sequence in fragments shorter than    1149 bp
  50% of sequence in fragments shorter than    1276 bp
  55% of sequence in fragments shorter than    1411 bp
  60% of sequence in fragments shorter than    1562 bp
  65% of sequence in fragments shorter than    1732 bp
  70% of sequence in fragments shorter than    1924 bp
  75% of sequence in fragments shorter than    2147 bp
  80% of sequence in fragments shorter than    2419 bp
  85% of sequence in fragments shorter than    2767 bp
  90% of sequence in fragments shorter than    3228 bp
  95% of sequence in fragments shorter than    4152 bp

# MlyI.PacI (GAGTC+TTAATTAA) double digest, 4090 thousand fragments (3931692+159455 sites), 728 bp average fragment length
   5% of sequence in fragments shorter than     255 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     490 bp
  20% of sequence in fragments shorter than     595 bp
  25% of sequence in fragments shorter than     699 bp
  30% of sequence in fragments shorter than     802 bp
  35% of sequence in fragments shorter than     907 bp
  40% of sequence in fragments shorter than    1016 bp
  45% of sequence in fragments shorter than    1132 bp
  50% of sequence in fragments shorter than    1258 bp
  55% of sequence in fragments shorter than    1392 bp
  60% of sequence in fragments shorter than    1541 bp
  65% of sequence in fragments shorter than    1706 bp
  70% of sequence in fragments shorter than    1892 bp
  75% of sequence in fragments shorter than    2119 bp
  80% of sequence in fragments shorter than    2402 bp
  85% of sequence in fragments shorter than    2775 bp
  90% of sequence in fragments shorter than    3305 bp
  95% of sequence in fragments shorter than    4316 bp

# Hpy99I.MlyI (CGWCG+GAGTC) double digest, 4089 thousand fragments (157917+3931692 sites), 728 bp average fragment length
   5% of sequence in fragments shorter than     252 bp
  10% of sequence in fragments shorter than     376 bp
  15% of sequence in fragments shorter than     489 bp
  20% of sequence in fragments shorter than     597 bp
  25% of sequence in fragments shorter than     702 bp
  30% of sequence in fragments shorter than     807 bp
  35% of sequence in fragments shorter than     915 bp
  40% of sequence in fragments shorter than    1027 bp
  45% of sequence in fragments shorter than    1146 bp
  50% of sequence in fragments shorter than    1277 bp
  55% of sequence in fragments shorter than    1415 bp
  60% of sequence in fragments shorter than    1570 bp
  65% of sequence in fragments shorter than    1738 bp
  70% of sequence in fragments shorter than    1936 bp
  75% of sequence in fragments shorter than    2173 bp
  80% of sequence in fragments shorter than    2471 bp
  85% of sequence in fragments shorter than    2855 bp
  90% of sequence in fragments shorter than    3410 bp
  95% of sequence in fragments shorter than    4452 bp

# SacII.Tsp45I (CCGCGG+GTSAC) double digest, 4088 thousand fragments (66901+4021761 sites), 728 bp average fragment length
   5% of sequence in fragments shorter than     253 bp
  10% of sequence in fragments shorter than     377 bp
  15% of sequence in fragments shorter than     492 bp
  20% of sequence in fragments shorter than     599 bp
  25% of sequence in fragments shorter than     703 bp
  30% of sequence in fragments shorter than     812 bp
  35% of sequence in fragments shorter than     919 bp
  40% of sequence in fragments shorter than    1033 bp
  45% of sequence in fragments shorter than    1153 bp
  50% of sequence in fragments shorter than    1280 bp
  55% of sequence in fragments shorter than    1419 bp
  60% of sequence in fragments shorter than    1574 bp
  65% of sequence in fragments shorter than    1744 bp
  70% of sequence in fragments shorter than    1940 bp
  75% of sequence in fragments shorter than    2175 bp
  80% of sequence in fragments shorter than    2461 bp
  85% of sequence in fragments shorter than    2840 bp
  90% of sequence in fragments shorter than    3398 bp
  95% of sequence in fragments shorter than    4384 bp

# BfiI.SspI (ACTGGG+AATATT) double digest, 4083 thousand fragments (1706594+2377267 sites), 729 bp average fragment length
   5% of sequence in fragments shorter than     254 bp
  10% of sequence in fragments shorter than     381 bp
  15% of sequence in fragments shorter than     497 bp
  20% of sequence in fragments shorter than     604 bp
  25% of sequence in fragments shorter than     708 bp
  30% of sequence in fragments shorter than     811 bp
  35% of sequence in fragments shorter than     918 bp
  40% of sequence in fragments shorter than    1028 bp
  45% of sequence in fragments shorter than    1136 bp
  50% of sequence in fragments shorter than    1258 bp
  55% of sequence in fragments shorter than    1387 bp
  60% of sequence in fragments shorter than    1530 bp
  65% of sequence in fragments shorter than    1690 bp
  70% of sequence in fragments shorter than    1872 bp
  75% of sequence in fragments shorter than    2082 bp
  80% of sequence in fragments shorter than    2344 bp
  85% of sequence in fragments shorter than    2681 bp
  90% of sequence in fragments shorter than    3123 bp
  95% of sequence in fragments shorter than    3968 bp

# BspHI.NspI (TCATGA+RCATGY) double digest, 4082 thousand fragments (978290+3104359 sites), 730 bp average fragment length
   5% of sequence in fragments shorter than     253 bp
  10% of sequence in fragments shorter than     390 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     613 bp
  25% of sequence in fragments shorter than     720 bp
  30% of sequence in fragments shorter than     827 bp
  35% of sequence in fragments shorter than     936 bp
  40% of sequence in fragments shorter than    1049 bp
  45% of sequence in fragments shorter than    1165 bp
  50% of sequence in fragments shorter than    1289 bp
  55% of sequence in fragments shorter than    1418 bp
  60% of sequence in fragments shorter than    1566 bp
  65% of sequence in fragments shorter than    1727 bp
  70% of sequence in fragments shorter than    1911 bp
  75% of sequence in fragments shorter than    2122 bp
  80% of sequence in fragments shorter than    2373 bp
  85% of sequence in fragments shorter than    2712 bp
  90% of sequence in fragments shorter than    3197 bp
  95% of sequence in fragments shorter than    4131 bp

# SfeI.SphI (CTRYAG+GCATGC) double digest, 4081 thousand fragments (3531009+550952 sites), 730 bp average fragment length
   5% of sequence in fragments shorter than     259 bp
  10% of sequence in fragments shorter than     388 bp
  15% of sequence in fragments shorter than     498 bp
  20% of sequence in fragments shorter than     604 bp
  25% of sequence in fragments shorter than     706 bp
  30% of sequence in fragments shorter than     813 bp
  35% of sequence in fragments shorter than     921 bp
  40% of sequence in fragments shorter than    1031 bp
  45% of sequence in fragments shorter than    1143 bp
  50% of sequence in fragments shorter than    1266 bp
  55% of sequence in fragments shorter than    1393 bp
  60% of sequence in fragments shorter than    1537 bp
  65% of sequence in fragments shorter than    1695 bp
  70% of sequence in fragments shorter than    1874 bp
  75% of sequence in fragments shorter than    2079 bp
  80% of sequence in fragments shorter than    2304 bp
  85% of sequence in fragments shorter than    2619 bp
  90% of sequence in fragments shorter than    3059 bp
  95% of sequence in fragments shorter than    3838 bp

# PspXI.Tsp45I (VCTCGAGB+GTSAC) double digest, 4080 thousand fragments (59193+4021761 sites), 730 bp average fragment length
   5% of sequence in fragments shorter than     254 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     493 bp
  20% of sequence in fragments shorter than     600 bp
  25% of sequence in fragments shorter than     704 bp
  30% of sequence in fragments shorter than     812 bp
  35% of sequence in fragments shorter than     918 bp
  40% of sequence in fragments shorter than    1032 bp
  45% of sequence in fragments shorter than    1152 bp
  50% of sequence in fragments shorter than    1278 bp
  55% of sequence in fragments shorter than    1416 bp
  60% of sequence in fragments shorter than    1571 bp
  65% of sequence in fragments shorter than    1740 bp
  70% of sequence in fragments shorter than    1935 bp
  75% of sequence in fragments shorter than    2168 bp
  80% of sequence in fragments shorter than    2453 bp
  85% of sequence in fragments shorter than    2828 bp
  90% of sequence in fragments shorter than    3379 bp
  95% of sequence in fragments shorter than    4358 bp

# AgeI.Tsp45I (ACCGGT+GTSAC) double digest, 4074 thousand fragments (53386+4021761 sites), 731 bp average fragment length
   5% of sequence in fragments shorter than     255 bp
  10% of sequence in fragments shorter than     379 bp
  15% of sequence in fragments shorter than     494 bp
  20% of sequence in fragments shorter than     601 bp
  25% of sequence in fragments shorter than     705 bp
  30% of sequence in fragments shorter than     814 bp
  35% of sequence in fragments shorter than     920 bp
  40% of sequence in fragments shorter than    1034 bp
  45% of sequence in fragments shorter than    1154 bp
  50% of sequence in fragments shorter than    1281 bp
  55% of sequence in fragments shorter than    1419 bp
  60% of sequence in fragments shorter than    1573 bp
  65% of sequence in fragments shorter than    1742 bp
  70% of sequence in fragments shorter than    1937 bp
  75% of sequence in fragments shorter than    2170 bp
  80% of sequence in fragments shorter than    2455 bp
  85% of sequence in fragments shorter than    2830 bp
  90% of sequence in fragments shorter than    3380 bp
  95% of sequence in fragments shorter than    4359 bp

# ScaI.SfeI (AGTACT+CTRYAG) double digest, 4074 thousand fragments (543793+3531009 sites), 731 bp average fragment length
   5% of sequence in fragments shorter than     258 bp
  10% of sequence in fragments shorter than     386 bp
  15% of sequence in fragments shorter than     496 bp
  20% of sequence in fragments shorter than     601 bp
  25% of sequence in fragments shorter than     701 bp
  30% of sequence in fragments shorter than     807 bp
  35% of sequence in fragments shorter than     913 bp
  40% of sequence in fragments shorter than    1023 bp
  45% of sequence in fragments shorter than    1132 bp
  50% of sequence in fragments shorter than    1252 bp
  55% of sequence in fragments shorter than    1380 bp
  60% of sequence in fragments shorter than    1517 bp
  65% of sequence in fragments shorter than    1672 bp
  70% of sequence in fragments shorter than    1845 bp
  75% of sequence in fragments shorter than    2045 bp
  80% of sequence in fragments shorter than    2261 bp
  85% of sequence in fragments shorter than    2570 bp
  90% of sequence in fragments shorter than    2995 bp
  95% of sequence in fragments shorter than    3748 bp

# HhaI.HpaII (GCGC+CCGG) double digest, 4073 thousand fragments (1756502+2317722 sites), 731 bp average fragment length
   5% of sequence in fragments shorter than     226 bp
  10% of sequence in fragments shorter than     429 bp
  15% of sequence in fragments shorter than     636 bp
  20% of sequence in fragments shorter than     829 bp
  25% of sequence in fragments shorter than    1036 bp
  30% of sequence in fragments shorter than    1264 bp
  35% of sequence in fragments shorter than    1509 bp
  40% of sequence in fragments shorter than    1769 bp
  45% of sequence in fragments shorter than    2053 bp
  50% of sequence in fragments shorter than    2372 bp
  55% of sequence in fragments shorter than    2723 bp
  60% of sequence in fragments shorter than    3121 bp
  65% of sequence in fragments shorter than    3571 bp
  70% of sequence in fragments shorter than    4096 bp
  75% of sequence in fragments shorter than    4705 bp
  80% of sequence in fragments shorter than    5459 bp
  85% of sequence in fragments shorter than    6424 bp
  90% of sequence in fragments shorter than    7807 bp
  95% of sequence in fragments shorter than   10183 bp

# AvaII.PstI (GGWCC+CTGCAG) double digest, 4068 thousand fragments (2747690+1321469 sites), 732 bp average fragment length
   5% of sequence in fragments shorter than     235 bp
  10% of sequence in fragments shorter than     371 bp
  15% of sequence in fragments shorter than     507 bp
  20% of sequence in fragments shorter than     638 bp
  25% of sequence in fragments shorter than     763 bp
  30% of sequence in fragments shorter than     896 bp
  35% of sequence in fragments shorter than    1027 bp
  40% of sequence in fragments shorter than    1174 bp
  45% of sequence in fragments shorter than    1330 bp
  50% of sequence in fragments shorter than    1496 bp
  55% of sequence in fragments shorter than    1682 bp
  60% of sequence in fragments shorter than    1882 bp
  65% of sequence in fragments shorter than    2101 bp
  70% of sequence in fragments shorter than    2361 bp
  75% of sequence in fragments shorter than    2663 bp
  80% of sequence in fragments shorter than    3032 bp
  85% of sequence in fragments shorter than    3508 bp
  90% of sequence in fragments shorter than    4191 bp
  95% of sequence in fragments shorter than    5425 bp

# MlyI.SnaBI (GAGTC+TACGTA) double digest, 4063 thousand fragments (3931692+132299 sites), 733 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     380 bp
  15% of sequence in fragments shorter than     493 bp
  20% of sequence in fragments shorter than     599 bp
  25% of sequence in fragments shorter than     703 bp
  30% of sequence in fragments shorter than     807 bp
  35% of sequence in fragments shorter than     912 bp
  40% of sequence in fragments shorter than    1022 bp
  45% of sequence in fragments shorter than    1139 bp
  50% of sequence in fragments shorter than    1267 bp
  55% of sequence in fragments shorter than    1399 bp
  60% of sequence in fragments shorter than    1549 bp
  65% of sequence in fragments shorter than    1715 bp
  70% of sequence in fragments shorter than    1902 bp
  75% of sequence in fragments shorter than    2130 bp
  80% of sequence in fragments shorter than    2415 bp
  85% of sequence in fragments shorter than    2780 bp
  90% of sequence in fragments shorter than    3304 bp
  95% of sequence in fragments shorter than    4276 bp

# PmeI.Tsp45I (GTTTAAAC+GTSAC) double digest, 4063 thousand fragments (42094+4021761 sites), 733 bp average fragment length
   5% of sequence in fragments shorter than     256 bp
  10% of sequence in fragments shorter than     380 bp
  15% of sequence in fragments shorter than     495 bp
  20% of sequence in fragments shorter than     602 bp
  25% of sequence in fragments shorter than     706 bp
  30% of sequence in fragments shorter than     814 bp
  35% of sequence in fragments shorter than     920 bp
  40% of sequence in fragments shorter than    1034 bp
  45% of sequence in fragments shorter than    1153 bp
  50% of sequence in fragments shorter than    1280 bp
  55% of sequence in fragments shorter than    1418 bp
  60% of sequence in fragments shorter than    1572 bp
  65% of sequence in fragments shorter than    1741 bp
  70% of sequence in fragments shorter than    1936 bp
  75% of sequence in fragments shorter than    2168 bp
  80% of sequence in fragments shorter than    2451 bp
  85% of sequence in fragments shorter than    2826 bp
  90% of sequence in fragments shorter than    3375 bp
  95% of sequence in fragments shorter than    4352 bp

# MlyI.NaeI (GAGTC+GCCGGC) double digest, 4056 thousand fragments (3931692+125692 sites), 734 bp average fragment length
   5% of sequence in fragments shorter than     254 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     492 bp
  20% of sequence in fragments shorter than     600 bp
  25% of sequence in fragments shorter than     706 bp
  30% of sequence in fragments shorter than     812 bp
  35% of sequence in fragments shorter than     920 bp
  40% of sequence in fragments shorter than    1033 bp
  45% of sequence in fragments shorter than    1154 bp
  50% of sequence in fragments shorter than    1285 bp
  55% of sequence in fragments shorter than    1425 bp
  60% of sequence in fragments shorter than    1580 bp
  65% of sequence in fragments shorter than    1751 bp
  70% of sequence in fragments shorter than    1950 bp
  75% of sequence in fragments shorter than    2190 bp
  80% of sequence in fragments shorter than    2491 bp
  85% of sequence in fragments shorter than    2882 bp
  90% of sequence in fragments shorter than    3447 bp
  95% of sequence in fragments shorter than    4510 bp

# BscAI.SpeI (GCATC+ACTAGT) double digest, 4052 thousand fragments (3652926+400286 sites), 735 bp average fragment length
   5% of sequence in fragments shorter than     252 bp
  10% of sequence in fragments shorter than     389 bp
  15% of sequence in fragments shorter than     511 bp
  20% of sequence in fragments shorter than     623 bp
  25% of sequence in fragments shorter than     727 bp
  30% of sequence in fragments shorter than     834 bp
  35% of sequence in fragments shorter than     940 bp
  40% of sequence in fragments shorter than    1056 bp
  45% of sequence in fragments shorter than    1177 bp
  50% of sequence in fragments shorter than    1301 bp
  55% of sequence in fragments shorter than    1433 bp
  60% of sequence in fragments shorter than    1579 bp
  65% of sequence in fragments shorter than    1736 bp
  70% of sequence in fragments shorter than    1922 bp
  75% of sequence in fragments shorter than    2134 bp
  80% of sequence in fragments shorter than    2394 bp
  85% of sequence in fragments shorter than    2727 bp
  90% of sequence in fragments shorter than    3199 bp
  95% of sequence in fragments shorter than    4053 bp

# SalI.Tsp45I (GTCGAC+GTSAC) double digest, 4052 thousand fragments (31396+4021761 sites), 735 bp average fragment length
   5% of sequence in fragments shorter than     256 bp
  10% of sequence in fragments shorter than     381 bp
  15% of sequence in fragments shorter than     496 bp
  20% of sequence in fragments shorter than     604 bp
  25% of sequence in fragments shorter than     708 bp
  30% of sequence in fragments shorter than     817 bp
  35% of sequence in fragments shorter than     923 bp
  40% of sequence in fragments shorter than    1037 bp
  45% of sequence in fragments shorter than    1158 bp
  50% of sequence in fragments shorter than    1285 bp
  55% of sequence in fragments shorter than    1424 bp
  60% of sequence in fragments shorter than    1578 bp
  65% of sequence in fragments shorter than    1749 bp
  70% of sequence in fragments shorter than    1944 bp
  75% of sequence in fragments shorter than    2178 bp
  80% of sequence in fragments shorter than    2464 bp
  85% of sequence in fragments shorter than    2841 bp
  90% of sequence in fragments shorter than    3395 bp
  95% of sequence in fragments shorter than    4377 bp

# MlyI.XhoI (GAGTC+CTCGAG) double digest, 4052 thousand fragments (3931692+121308 sites), 735 bp average fragment length
   5% of sequence in fragments shorter than     256 bp
  10% of sequence in fragments shorter than     381 bp
  15% of sequence in fragments shorter than     495 bp
  20% of sequence in fragments shorter than     601 bp
  25% of sequence in fragments shorter than     706 bp
  30% of sequence in fragments shorter than     812 bp
  35% of sequence in fragments shorter than     918 bp
  40% of sequence in fragments shorter than    1029 bp
  45% of sequence in fragments shorter than    1148 bp
  50% of sequence in fragments shorter than    1278 bp
  55% of sequence in fragments shorter than    1415 bp
  60% of sequence in fragments shorter than    1570 bp
  65% of sequence in fragments shorter than    1741 bp
  70% of sequence in fragments shorter than    1940 bp
  75% of sequence in fragments shorter than    2177 bp
  80% of sequence in fragments shorter than    2475 bp
  85% of sequence in fragments shorter than    2861 bp
  90% of sequence in fragments shorter than    3420 bp
  95% of sequence in fragments shorter than    4477 bp

# PstI.SmlI (CTGCAG+CTYRAG) double digest, 4048 thousand fragments (1321469+2727867 sites), 736 bp average fragment length
   5% of sequence in fragments shorter than     245 bp
  10% of sequence in fragments shorter than     385 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     619 bp
  25% of sequence in fragments shorter than     726 bp
  30% of sequence in fragments shorter than     836 bp
  35% of sequence in fragments shorter than     950 bp
  40% of sequence in fragments shorter than    1068 bp
  45% of sequence in fragments shorter than    1193 bp
  50% of sequence in fragments shorter than    1327 bp
  55% of sequence in fragments shorter than    1467 bp
  60% of sequence in fragments shorter than    1626 bp
  65% of sequence in fragments shorter than    1801 bp
  70% of sequence in fragments shorter than    1998 bp
  75% of sequence in fragments shorter than    2223 bp
  80% of sequence in fragments shorter than    2500 bp
  85% of sequence in fragments shorter than    2817 bp
  90% of sequence in fragments shorter than    3310 bp
  95% of sequence in fragments shorter than    4191 bp

# AvaIII.StyI (ATGCAT+CCWWGG) double digest, 4046 thousand fragments (933357+3114107 sites), 736 bp average fragment length
   5% of sequence in fragments shorter than     254 bp
  10% of sequence in fragments shorter than     386 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     609 bp
  25% of sequence in fragments shorter than     715 bp
  30% of sequence in fragments shorter than     817 bp
  35% of sequence in fragments shorter than     928 bp
  40% of sequence in fragments shorter than    1041 bp
  45% of sequence in fragments shorter than    1162 bp
  50% of sequence in fragments shorter than    1288 bp
  55% of sequence in fragments shorter than    1423 bp
  60% of sequence in fragments shorter than    1573 bp
  65% of sequence in fragments shorter than    1741 bp
  70% of sequence in fragments shorter than    1931 bp
  75% of sequence in fragments shorter than    2151 bp
  80% of sequence in fragments shorter than    2420 bp
  85% of sequence in fragments shorter than    2764 bp
  90% of sequence in fragments shorter than    3226 bp
  95% of sequence in fragments shorter than    4136 bp

# MluI.Tsp45I (ACGCGT+GTSAC) double digest, 4042 thousand fragments (21415+4021761 sites), 737 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     382 bp
  15% of sequence in fragments shorter than     498 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     710 bp
  30% of sequence in fragments shorter than     819 bp
  35% of sequence in fragments shorter than     927 bp
  40% of sequence in fragments shorter than    1040 bp
  45% of sequence in fragments shorter than    1161 bp
  50% of sequence in fragments shorter than    1290 bp
  55% of sequence in fragments shorter than    1429 bp
  60% of sequence in fragments shorter than    1583 bp
  65% of sequence in fragments shorter than    1755 bp
  70% of sequence in fragments shorter than    1951 bp
  75% of sequence in fragments shorter than    2186 bp
  80% of sequence in fragments shorter than    2473 bp
  85% of sequence in fragments shorter than    2851 bp
  90% of sequence in fragments shorter than    3407 bp
  95% of sequence in fragments shorter than    4391 bp

# BscAI.HpaI (GCATC+GTTAAC) double digest, 4041 thousand fragments (3652926+389418 sites), 737 bp average fragment length
   5% of sequence in fragments shorter than     252 bp
  10% of sequence in fragments shorter than     389 bp
  15% of sequence in fragments shorter than     511 bp
  20% of sequence in fragments shorter than     620 bp
  25% of sequence in fragments shorter than     725 bp
  30% of sequence in fragments shorter than     833 bp
  35% of sequence in fragments shorter than     946 bp
  40% of sequence in fragments shorter than    1063 bp
  45% of sequence in fragments shorter than    1182 bp
  50% of sequence in fragments shorter than    1308 bp
  55% of sequence in fragments shorter than    1443 bp
  60% of sequence in fragments shorter than    1591 bp
  65% of sequence in fragments shorter than    1753 bp
  70% of sequence in fragments shorter than    1940 bp
  75% of sequence in fragments shorter than    2159 bp
  80% of sequence in fragments shorter than    2420 bp
  85% of sequence in fragments shorter than    2761 bp
  90% of sequence in fragments shorter than    3247 bp
  95% of sequence in fragments shorter than    4139 bp

# StuI.StyI (AGGCCT+CCWWGG) double digest, 4039 thousand fragments (925729+3114107 sites), 737 bp average fragment length
   5% of sequence in fragments shorter than     254 bp
  10% of sequence in fragments shorter than     378 bp
  15% of sequence in fragments shorter than     502 bp
  20% of sequence in fragments shorter than     618 bp
  25% of sequence in fragments shorter than     717 bp
  30% of sequence in fragments shorter than     828 bp
  35% of sequence in fragments shorter than     950 bp
  40% of sequence in fragments shorter than    1075 bp
  45% of sequence in fragments shorter than    1211 bp
  50% of sequence in fragments shorter than    1356 bp
  55% of sequence in fragments shorter than    1511 bp
  60% of sequence in fragments shorter than    1686 bp
  65% of sequence in fragments shorter than    1878 bp
  70% of sequence in fragments shorter than    2086 bp
  75% of sequence in fragments shorter than    2343 bp
  80% of sequence in fragments shorter than    2644 bp
  85% of sequence in fragments shorter than    3039 bp
  90% of sequence in fragments shorter than    3596 bp
  95% of sequence in fragments shorter than    4553 bp

# AvaIII.NspI (ATGCAT+RCATGY) double digest, 4037 thousand fragments (933357+3104359 sites), 738 bp average fragment length
   5% of sequence in fragments shorter than     270 bp
  10% of sequence in fragments shorter than     416 bp
  15% of sequence in fragments shorter than     544 bp
  20% of sequence in fragments shorter than     666 bp
  25% of sequence in fragments shorter than     781 bp
  30% of sequence in fragments shorter than     899 bp
  35% of sequence in fragments shorter than    1020 bp
  40% of sequence in fragments shorter than    1144 bp
  45% of sequence in fragments shorter than    1273 bp
  50% of sequence in fragments shorter than    1412 bp
  55% of sequence in fragments shorter than    1562 bp
  60% of sequence in fragments shorter than    1724 bp
  65% of sequence in fragments shorter than    1905 bp
  70% of sequence in fragments shorter than    2107 bp
  75% of sequence in fragments shorter than    2346 bp
  80% of sequence in fragments shorter than    2640 bp
  85% of sequence in fragments shorter than    3015 bp
  90% of sequence in fragments shorter than    3544 bp
  95% of sequence in fragments shorter than    4562 bp

# SgrAI.Tsp45I (CRCCGGYG+GTSAC) double digest, 4036 thousand fragments (15270+4021761 sites), 738 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     382 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     711 bp
  30% of sequence in fragments shorter than     820 bp
  35% of sequence in fragments shorter than     928 bp
  40% of sequence in fragments shorter than    1041 bp
  45% of sequence in fragments shorter than    1163 bp
  50% of sequence in fragments shorter than    1291 bp
  55% of sequence in fragments shorter than    1430 bp
  60% of sequence in fragments shorter than    1585 bp
  65% of sequence in fragments shorter than    1757 bp
  70% of sequence in fragments shorter than    1954 bp
  75% of sequence in fragments shorter than    2189 bp
  80% of sequence in fragments shorter than    2476 bp
  85% of sequence in fragments shorter than    2856 bp
  90% of sequence in fragments shorter than    3412 bp
  95% of sequence in fragments shorter than    4398 bp

# NruI.Tsp45I (TCGCGA+GTSAC) double digest, 4036 thousand fragments (14872+4021761 sites), 738 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     382 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     711 bp
  30% of sequence in fragments shorter than     820 bp
  35% of sequence in fragments shorter than     928 bp
  40% of sequence in fragments shorter than    1041 bp
  45% of sequence in fragments shorter than    1163 bp
  50% of sequence in fragments shorter than    1291 bp
  55% of sequence in fragments shorter than    1430 bp
  60% of sequence in fragments shorter than    1584 bp
  65% of sequence in fragments shorter than    1756 bp
  70% of sequence in fragments shorter than    1953 bp
  75% of sequence in fragments shorter than    2187 bp
  80% of sequence in fragments shorter than    2474 bp
  85% of sequence in fragments shorter than    2853 bp
  90% of sequence in fragments shorter than    3409 bp
  95% of sequence in fragments shorter than    4392 bp

# PvuI.Tsp45I (CGATCG+GTSAC) double digest, 4035 thousand fragments (14186+4021761 sites), 738 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     383 bp
  15% of sequence in fragments shorter than     498 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     711 bp
  30% of sequence in fragments shorter than     820 bp
  35% of sequence in fragments shorter than     927 bp
  40% of sequence in fragments shorter than    1042 bp
  45% of sequence in fragments shorter than    1163 bp
  50% of sequence in fragments shorter than    1291 bp
  55% of sequence in fragments shorter than    1431 bp
  60% of sequence in fragments shorter than    1586 bp
  65% of sequence in fragments shorter than    1757 bp
  70% of sequence in fragments shorter than    1954 bp
  75% of sequence in fragments shorter than    2189 bp
  80% of sequence in fragments shorter than    2476 bp
  85% of sequence in fragments shorter than    2854 bp
  90% of sequence in fragments shorter than    3409 bp
  95% of sequence in fragments shorter than    4391 bp

# FseI.Tsp45I (GGCCGGCC+GTSAC) double digest, 4034 thousand fragments (13662+4021761 sites), 738 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     382 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     711 bp
  30% of sequence in fragments shorter than     820 bp
  35% of sequence in fragments shorter than     928 bp
  40% of sequence in fragments shorter than    1042 bp
  45% of sequence in fragments shorter than    1163 bp
  50% of sequence in fragments shorter than    1292 bp
  55% of sequence in fragments shorter than    1431 bp
  60% of sequence in fragments shorter than    1586 bp
  65% of sequence in fragments shorter than    1758 bp
  70% of sequence in fragments shorter than    1955 bp
  75% of sequence in fragments shorter than    2190 bp
  80% of sequence in fragments shorter than    2478 bp
  85% of sequence in fragments shorter than    2857 bp
  90% of sequence in fragments shorter than    3414 bp
  95% of sequence in fragments shorter than    4399 bp

# NotI.Tsp45I (GCGGCCGC+GTSAC) double digest, 4031 thousand fragments (9795+4021761 sites), 739 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     383 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     607 bp
  25% of sequence in fragments shorter than     712 bp
  30% of sequence in fragments shorter than     821 bp
  35% of sequence in fragments shorter than     929 bp
  40% of sequence in fragments shorter than    1042 bp
  45% of sequence in fragments shorter than    1164 bp
  50% of sequence in fragments shorter than    1292 bp
  55% of sequence in fragments shorter than    1432 bp
  60% of sequence in fragments shorter than    1587 bp
  65% of sequence in fragments shorter than    1759 bp
  70% of sequence in fragments shorter than    1956 bp
  75% of sequence in fragments shorter than    2191 bp
  80% of sequence in fragments shorter than    2479 bp
  85% of sequence in fragments shorter than    2858 bp
  90% of sequence in fragments shorter than    3415 bp
  95% of sequence in fragments shorter than    4401 bp

# RsrII.Tsp45I (CGGWCCG+GTSAC) double digest, 4031 thousand fragments (9764+4021761 sites), 739 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     383 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     607 bp
  25% of sequence in fragments shorter than     712 bp
  30% of sequence in fragments shorter than     821 bp
  35% of sequence in fragments shorter than     929 bp
  40% of sequence in fragments shorter than    1043 bp
  45% of sequence in fragments shorter than    1164 bp
  50% of sequence in fragments shorter than    1293 bp
  55% of sequence in fragments shorter than    1432 bp
  60% of sequence in fragments shorter than    1587 bp
  65% of sequence in fragments shorter than    1759 bp
  70% of sequence in fragments shorter than    1956 bp
  75% of sequence in fragments shorter than    2191 bp
  80% of sequence in fragments shorter than    2479 bp
  85% of sequence in fragments shorter than    2858 bp
  90% of sequence in fragments shorter than    3415 bp
  95% of sequence in fragments shorter than    4399 bp

# BscAI.SapI (GCATC+GCTCTTC) double digest, 4029 thousand fragments (3652926+377161 sites), 739 bp average fragment length
   5% of sequence in fragments shorter than     252 bp
  10% of sequence in fragments shorter than     387 bp
  15% of sequence in fragments shorter than     510 bp
  20% of sequence in fragments shorter than     623 bp
  25% of sequence in fragments shorter than     732 bp
  30% of sequence in fragments shorter than     842 bp
  35% of sequence in fragments shorter than     957 bp
  40% of sequence in fragments shorter than    1078 bp
  45% of sequence in fragments shorter than    1204 bp
  50% of sequence in fragments shorter than    1332 bp
  55% of sequence in fragments shorter than    1470 bp
  60% of sequence in fragments shorter than    1621 bp
  65% of sequence in fragments shorter than    1793 bp
  70% of sequence in fragments shorter than    1989 bp
  75% of sequence in fragments shorter than    2218 bp
  80% of sequence in fragments shorter than    2493 bp
  85% of sequence in fragments shorter than    2847 bp
  90% of sequence in fragments shorter than    3357 bp
  95% of sequence in fragments shorter than    4286 bp

# NspI.StuI (RCATGY+AGGCCT) double digest, 4029 thousand fragments (3104359+925729 sites), 739 bp average fragment length
   5% of sequence in fragments shorter than     258 bp
  10% of sequence in fragments shorter than     387 bp
  15% of sequence in fragments shorter than     511 bp
  20% of sequence in fragments shorter than     623 bp
  25% of sequence in fragments shorter than     723 bp
  30% of sequence in fragments shorter than     835 bp
  35% of sequence in fragments shorter than     943 bp
  40% of sequence in fragments shorter than    1059 bp
  45% of sequence in fragments shorter than    1181 bp
  50% of sequence in fragments shorter than    1309 bp
  55% of sequence in fragments shorter than    1441 bp
  60% of sequence in fragments shorter than    1589 bp
  65% of sequence in fragments shorter than    1751 bp
  70% of sequence in fragments shorter than    1922 bp
  75% of sequence in fragments shorter than    2128 bp
  80% of sequence in fragments shorter than    2380 bp
  85% of sequence in fragments shorter than    2702 bp
  90% of sequence in fragments shorter than    3139 bp
  95% of sequence in fragments shorter than    3847 bp

# BscAI.SmaI (GCATC+CCCGGG) double digest, 4029 thousand fragments (3652926+376940 sites), 739 bp average fragment length
   5% of sequence in fragments shorter than     253 bp
  10% of sequence in fragments shorter than     388 bp
  15% of sequence in fragments shorter than     510 bp
  20% of sequence in fragments shorter than     621 bp
  25% of sequence in fragments shorter than     727 bp
  30% of sequence in fragments shorter than     835 bp
  35% of sequence in fragments shorter than     950 bp
  40% of sequence in fragments shorter than    1069 bp
  45% of sequence in fragments shorter than    1192 bp
  50% of sequence in fragments shorter than    1321 bp
  55% of sequence in fragments shorter than    1459 bp
  60% of sequence in fragments shorter than    1604 bp
  65% of sequence in fragments shorter than    1773 bp
  70% of sequence in fragments shorter than    1964 bp
  75% of sequence in fragments shorter than    2187 bp
  80% of sequence in fragments shorter than    2457 bp
  85% of sequence in fragments shorter than    2805 bp
  90% of sequence in fragments shorter than    3304 bp
  95% of sequence in fragments shorter than    4217 bp

# ApaLI.SfeI (GTGCAC+CTRYAG) double digest, 4026 thousand fragments (496255+3531009 sites), 740 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     393 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     616 bp
  25% of sequence in fragments shorter than     719 bp
  30% of sequence in fragments shorter than     829 bp
  35% of sequence in fragments shorter than     934 bp
  40% of sequence in fragments shorter than    1044 bp
  45% of sequence in fragments shorter than    1160 bp
  50% of sequence in fragments shorter than    1286 bp
  55% of sequence in fragments shorter than    1408 bp
  60% of sequence in fragments shorter than    1555 bp
  65% of sequence in fragments shorter than    1715 bp
  70% of sequence in fragments shorter than    1895 bp
  75% of sequence in fragments shorter than    2102 bp
  80% of sequence in fragments shorter than    2330 bp
  85% of sequence in fragments shorter than    2649 bp
  90% of sequence in fragments shorter than    3098 bp
  95% of sequence in fragments shorter than    3882 bp

# AscI.Tsp45I (GGCGCGCC+GTSAC) double digest, 4026 thousand fragments (4751+4021761 sites), 740 bp average fragment length
   5% of sequence in fragments shorter than     258 bp
  10% of sequence in fragments shorter than     383 bp
  15% of sequence in fragments shorter than     500 bp
  20% of sequence in fragments shorter than     608 bp
  25% of sequence in fragments shorter than     713 bp
  30% of sequence in fragments shorter than     822 bp
  35% of sequence in fragments shorter than     930 bp
  40% of sequence in fragments shorter than    1044 bp
  45% of sequence in fragments shorter than    1165 bp
  50% of sequence in fragments shorter than    1294 bp
  55% of sequence in fragments shorter than    1434 bp
  60% of sequence in fragments shorter than    1589 bp
  65% of sequence in fragments shorter than    1761 bp
  70% of sequence in fragments shorter than    1958 bp
  75% of sequence in fragments shorter than    2193 bp
  80% of sequence in fragments shorter than    2481 bp
  85% of sequence in fragments shorter than    2861 bp
  90% of sequence in fragments shorter than    3418 bp
  95% of sequence in fragments shorter than    4403 bp

# PsiI.SspI (TTATAA+AATATT) double digest, 4024 thousand fragments (1647912+2377267 sites), 740 bp average fragment length
   5% of sequence in fragments shorter than     240 bp
  10% of sequence in fragments shorter than     382 bp
  15% of sequence in fragments shorter than     511 bp
  20% of sequence in fragments shorter than     635 bp
  25% of sequence in fragments shorter than     756 bp
  30% of sequence in fragments shorter than     887 bp
  35% of sequence in fragments shorter than    1024 bp
  40% of sequence in fragments shorter than    1169 bp
  45% of sequence in fragments shorter than    1329 bp
  50% of sequence in fragments shorter than    1498 bp
  55% of sequence in fragments shorter than    1694 bp
  60% of sequence in fragments shorter than    1913 bp
  65% of sequence in fragments shorter than    2169 bp
  70% of sequence in fragments shorter than    2481 bp
  75% of sequence in fragments shorter than    2869 bp
  80% of sequence in fragments shorter than    3358 bp
  85% of sequence in fragments shorter than    4063 bp
  90% of sequence in fragments shorter than    5279 bp
  95% of sequence in fragments shorter than    8343 bp

# BfiI.HpaII (ACTGGG+CCGG) double digest, 4023 thousand fragments (1706594+2317722 sites), 740 bp average fragment length
   5% of sequence in fragments shorter than     228 bp
  10% of sequence in fragments shorter than     384 bp
  15% of sequence in fragments shorter than     533 bp
  20% of sequence in fragments shorter than     678 bp
  25% of sequence in fragments shorter than     820 bp
  30% of sequence in fragments shorter than     964 bp
  35% of sequence in fragments shorter than    1117 bp
  40% of sequence in fragments shorter than    1280 bp
  45% of sequence in fragments shorter than    1455 bp
  50% of sequence in fragments shorter than    1644 bp
  55% of sequence in fragments shorter than    1848 bp
  60% of sequence in fragments shorter than    2068 bp
  65% of sequence in fragments shorter than    2318 bp
  70% of sequence in fragments shorter than    2618 bp
  75% of sequence in fragments shorter than    2952 bp
  80% of sequence in fragments shorter than    3383 bp
  85% of sequence in fragments shorter than    3954 bp
  90% of sequence in fragments shorter than    4774 bp
  95% of sequence in fragments shorter than    6300 bp

# BamHI.BscAI (GGATCC+GCATC) double digest, 4018 thousand fragments (365999+3652926 sites), 741 bp average fragment length
   5% of sequence in fragments shorter than     248 bp
  10% of sequence in fragments shorter than     388 bp
  15% of sequence in fragments shorter than     513 bp
  20% of sequence in fragments shorter than     626 bp
  25% of sequence in fragments shorter than     734 bp
  30% of sequence in fragments shorter than     846 bp
  35% of sequence in fragments shorter than     963 bp
  40% of sequence in fragments shorter than    1083 bp
  45% of sequence in fragments shorter than    1208 bp
  50% of sequence in fragments shorter than    1338 bp
  55% of sequence in fragments shorter than    1477 bp
  60% of sequence in fragments shorter than    1626 bp
  65% of sequence in fragments shorter than    1797 bp
  70% of sequence in fragments shorter than    1990 bp
  75% of sequence in fragments shorter than    2214 bp
  80% of sequence in fragments shorter than    2488 bp
  85% of sequence in fragments shorter than    2841 bp
  90% of sequence in fragments shorter than    3344 bp
  95% of sequence in fragments shorter than    4266 bp

# ClaI.MlyI (ATCGAT+GAGTC) double digest, 4017 thousand fragments (86477+3931692 sites), 741 bp average fragment length
   5% of sequence in fragments shorter than     259 bp
  10% of sequence in fragments shorter than     384 bp
  15% of sequence in fragments shorter than     498 bp
  20% of sequence in fragments shorter than     606 bp
  25% of sequence in fragments shorter than     711 bp
  30% of sequence in fragments shorter than     817 bp
  35% of sequence in fragments shorter than     923 bp
  40% of sequence in fragments shorter than    1035 bp
  45% of sequence in fragments shorter than    1154 bp
  50% of sequence in fragments shorter than    1282 bp
  55% of sequence in fragments shorter than    1419 bp
  60% of sequence in fragments shorter than    1571 bp
  65% of sequence in fragments shorter than    1738 bp
  70% of sequence in fragments shorter than    1933 bp
  75% of sequence in fragments shorter than    2166 bp
  80% of sequence in fragments shorter than    2458 bp
  85% of sequence in fragments shorter than    2834 bp
  90% of sequence in fragments shorter than    3373 bp
  95% of sequence in fragments shorter than    4399 bp

# NdeI.StyI (CATATG+CCWWGG) double digest, 4017 thousand fragments (903360+3114107 sites), 741 bp average fragment length
   5% of sequence in fragments shorter than     256 bp
  10% of sequence in fragments shorter than     382 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     610 bp
  25% of sequence in fragments shorter than     719 bp
  30% of sequence in fragments shorter than     823 bp
  35% of sequence in fragments shorter than     936 bp
  40% of sequence in fragments shorter than    1052 bp
  45% of sequence in fragments shorter than    1172 bp
  50% of sequence in fragments shorter than    1303 bp
  55% of sequence in fragments shorter than    1440 bp
  60% of sequence in fragments shorter than    1593 bp
  65% of sequence in fragments shorter than    1763 bp
  70% of sequence in fragments shorter than    1956 bp
  75% of sequence in fragments shorter than    2182 bp
  80% of sequence in fragments shorter than    2458 bp
  85% of sequence in fragments shorter than    2805 bp
  90% of sequence in fragments shorter than    3286 bp
  95% of sequence in fragments shorter than    4243 bp

# NdeI.NspI (CATATG+RCATGY) double digest, 4007 thousand fragments (903360+3104359 sites), 743 bp average fragment length
   5% of sequence in fragments shorter than     261 bp
  10% of sequence in fragments shorter than     398 bp
  15% of sequence in fragments shorter than     520 bp
  20% of sequence in fragments shorter than     637 bp
  25% of sequence in fragments shorter than     749 bp
  30% of sequence in fragments shorter than     854 bp
  35% of sequence in fragments shorter than     970 bp
  40% of sequence in fragments shorter than    1086 bp
  45% of sequence in fragments shorter than    1210 bp
  50% of sequence in fragments shorter than    1343 bp
  55% of sequence in fragments shorter than    1485 bp
  60% of sequence in fragments shorter than    1640 bp
  65% of sequence in fragments shorter than    1812 bp
  70% of sequence in fragments shorter than    2007 bp
  75% of sequence in fragments shorter than    2233 bp
  80% of sequence in fragments shorter than    2510 bp
  85% of sequence in fragments shorter than    2862 bp
  90% of sequence in fragments shorter than    3384 bp
  95% of sequence in fragments shorter than    4388 bp

# AatII.MlyI (GACGTC+GAGTC) double digest, 4002 thousand fragments (71089+3931692 sites), 744 bp average fragment length
   5% of sequence in fragments shorter than     259 bp
  10% of sequence in fragments shorter than     384 bp
  15% of sequence in fragments shorter than     499 bp
  20% of sequence in fragments shorter than     608 bp
  25% of sequence in fragments shorter than     714 bp
  30% of sequence in fragments shorter than     821 bp
  35% of sequence in fragments shorter than     929 bp
  40% of sequence in fragments shorter than    1042 bp
  45% of sequence in fragments shorter than    1163 bp
  50% of sequence in fragments shorter than    1293 bp
  55% of sequence in fragments shorter than    1433 bp
  60% of sequence in fragments shorter than    1587 bp
  65% of sequence in fragments shorter than    1757 bp
  70% of sequence in fragments shorter than    1956 bp
  75% of sequence in fragments shorter than    2193 bp
  80% of sequence in fragments shorter than    2492 bp
  85% of sequence in fragments shorter than    2879 bp
  90% of sequence in fragments shorter than    3439 bp
  95% of sequence in fragments shorter than    4491 bp

# MlyI.SacII (GAGTC+CCGCGG) double digest, 3998 thousand fragments (3931692+66901 sites), 745 bp average fragment length
   5% of sequence in fragments shorter than     259 bp
  10% of sequence in fragments shorter than     385 bp
  15% of sequence in fragments shorter than     500 bp
  20% of sequence in fragments shorter than     610 bp
  25% of sequence in fragments shorter than     717 bp
  30% of sequence in fragments shorter than     824 bp
  35% of sequence in fragments shorter than     933 bp
  40% of sequence in fragments shorter than    1047 bp
  45% of sequence in fragments shorter than    1169 bp
  50% of sequence in fragments shorter than    1302 bp
  55% of sequence in fragments shorter than    1442 bp
  60% of sequence in fragments shorter than    1599 bp
  65% of sequence in fragments shorter than    1771 bp
  70% of sequence in fragments shorter than    1972 bp
  75% of sequence in fragments shorter than    2212 bp
  80% of sequence in fragments shorter than    2516 bp
  85% of sequence in fragments shorter than    2907 bp
  90% of sequence in fragments shorter than    3475 bp
  95% of sequence in fragments shorter than    4541 bp

# ApaI.SfeI (GGGCCC+CTRYAG) double digest, 3990 thousand fragments (460339+3531009 sites), 746 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     391 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     620 bp
  25% of sequence in fragments shorter than     726 bp
  30% of sequence in fragments shorter than     839 bp
  35% of sequence in fragments shorter than     953 bp
  40% of sequence in fragments shorter than    1068 bp
  45% of sequence in fragments shorter than    1190 bp
  50% of sequence in fragments shorter than    1321 bp
  55% of sequence in fragments shorter than    1456 bp
  60% of sequence in fragments shorter than    1609 bp
  65% of sequence in fragments shorter than    1777 bp
  70% of sequence in fragments shorter than    1966 bp
  75% of sequence in fragments shorter than    2167 bp
  80% of sequence in fragments shorter than    2434 bp
  85% of sequence in fragments shorter than    2773 bp
  90% of sequence in fragments shorter than    3252 bp
  95% of sequence in fragments shorter than    4099 bp

# MlyI.PspXI (GAGTC+VCTCGAGB) double digest, 3990 thousand fragments (3931692+59193 sites), 746 bp average fragment length
   5% of sequence in fragments shorter than     260 bp
  10% of sequence in fragments shorter than     386 bp
  15% of sequence in fragments shorter than     502 bp
  20% of sequence in fragments shorter than     611 bp
  25% of sequence in fragments shorter than     718 bp
  30% of sequence in fragments shorter than     824 bp
  35% of sequence in fragments shorter than     933 bp
  40% of sequence in fragments shorter than    1046 bp
  45% of sequence in fragments shorter than    1168 bp
  50% of sequence in fragments shorter than    1299 bp
  55% of sequence in fragments shorter than    1439 bp
  60% of sequence in fragments shorter than    1595 bp
  65% of sequence in fragments shorter than    1766 bp
  70% of sequence in fragments shorter than    1965 bp
  75% of sequence in fragments shorter than    2203 bp
  80% of sequence in fragments shorter than    2504 bp
  85% of sequence in fragments shorter than    2893 bp
  90% of sequence in fragments shorter than    3456 bp
  95% of sequence in fragments shorter than    4519 bp

# BscAI.BstEII (GCATC+GGTNACC) double digest, 3988 thousand fragments (3652926+335865 sites), 747 bp average fragment length
   5% of sequence in fragments shorter than     254 bp
  10% of sequence in fragments shorter than     391 bp
  15% of sequence in fragments shorter than     516 bp
  20% of sequence in fragments shorter than     630 bp
  25% of sequence in fragments shorter than     740 bp
  30% of sequence in fragments shorter than     850 bp
  35% of sequence in fragments shorter than     968 bp
  40% of sequence in fragments shorter than    1088 bp
  45% of sequence in fragments shorter than    1214 bp
  50% of sequence in fragments shorter than    1345 bp
  55% of sequence in fragments shorter than    1486 bp
  60% of sequence in fragments shorter than    1633 bp
  65% of sequence in fragments shorter than    1806 bp
  70% of sequence in fragments shorter than    2000 bp
  75% of sequence in fragments shorter than    2230 bp
  80% of sequence in fragments shorter than    2508 bp
  85% of sequence in fragments shorter than    2865 bp
  90% of sequence in fragments shorter than    3379 bp
  95% of sequence in fragments shorter than    4328 bp

# AgeI.MlyI (ACCGGT+GAGTC) double digest, 3984 thousand fragments (53386+3931692 sites), 747 bp average fragment length
   5% of sequence in fragments shorter than     260 bp
  10% of sequence in fragments shorter than     386 bp
  15% of sequence in fragments shorter than     501 bp
  20% of sequence in fragments shorter than     611 bp
  25% of sequence in fragments shorter than     717 bp
  30% of sequence in fragments shorter than     824 bp
  35% of sequence in fragments shorter than     932 bp
  40% of sequence in fragments shorter than    1045 bp
  45% of sequence in fragments shorter than    1167 bp
  50% of sequence in fragments shorter than    1298 bp
  55% of sequence in fragments shorter than    1437 bp
  60% of sequence in fragments shorter than    1591 bp
  65% of sequence in fragments shorter than    1761 bp
  70% of sequence in fragments shorter than    1962 bp
  75% of sequence in fragments shorter than    2200 bp
  80% of sequence in fragments shorter than    2499 bp
  85% of sequence in fragments shorter than    2887 bp
  90% of sequence in fragments shorter than    3447 bp
  95% of sequence in fragments shorter than    4500 bp

# EsaBC3I.SspI (TCGA+AATATT) double digest, 3980 thousand fragments (1603346+2377267 sites), 748 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     391 bp
  15% of sequence in fragments shorter than     508 bp
  20% of sequence in fragments shorter than     622 bp
  25% of sequence in fragments shorter than     731 bp
  30% of sequence in fragments shorter than     843 bp
  35% of sequence in fragments shorter than     957 bp
  40% of sequence in fragments shorter than    1077 bp
  45% of sequence in fragments shorter than    1202 bp
  50% of sequence in fragments shorter than    1331 bp
  55% of sequence in fragments shorter than    1475 bp
  60% of sequence in fragments shorter than    1634 bp
  65% of sequence in fragments shorter than    1814 bp
  70% of sequence in fragments shorter than    2016 bp
  75% of sequence in fragments shorter than    2256 bp
  80% of sequence in fragments shorter than    2549 bp
  85% of sequence in fragments shorter than    2916 bp
  90% of sequence in fragments shorter than    3428 bp
  95% of sequence in fragments shorter than    4341 bp

# AvaII.BscGI (GGWCC+CCCGT) double digest, 3978 thousand fragments (2747690+1231070 sites), 749 bp average fragment length
   5% of sequence in fragments shorter than     246 bp
  10% of sequence in fragments shorter than     364 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     643 bp
  25% of sequence in fragments shorter than     779 bp
  30% of sequence in fragments shorter than     917 bp
  35% of sequence in fragments shorter than    1056 bp
  40% of sequence in fragments shorter than    1212 bp
  45% of sequence in fragments shorter than    1379 bp
  50% of sequence in fragments shorter than    1557 bp
  55% of sequence in fragments shorter than    1750 bp
  60% of sequence in fragments shorter than    1962 bp
  65% of sequence in fragments shorter than    2200 bp
  70% of sequence in fragments shorter than    2469 bp
  75% of sequence in fragments shorter than    2786 bp
  80% of sequence in fragments shorter than    3177 bp
  85% of sequence in fragments shorter than    3673 bp
  90% of sequence in fragments shorter than    4375 bp
  95% of sequence in fragments shorter than    5595 bp

# EcoRV.SfeI (GATATC+CTRYAG) double digest, 3977 thousand fragments (446473+3531009 sites), 749 bp average fragment length
   5% of sequence in fragments shorter than     265 bp
  10% of sequence in fragments shorter than     397 bp
  15% of sequence in fragments shorter than     508 bp
  20% of sequence in fragments shorter than     619 bp
  25% of sequence in fragments shorter than     716 bp
  30% of sequence in fragments shorter than     825 bp
  35% of sequence in fragments shorter than     934 bp
  40% of sequence in fragments shorter than    1043 bp
  45% of sequence in fragments shorter than    1157 bp
  50% of sequence in fragments shorter than    1279 bp
  55% of sequence in fragments shorter than    1408 bp
  60% of sequence in fragments shorter than    1539 bp
  65% of sequence in fragments shorter than    1695 bp
  70% of sequence in fragments shorter than    1869 bp
  75% of sequence in fragments shorter than    2070 bp
  80% of sequence in fragments shorter than    2309 bp
  85% of sequence in fragments shorter than    2620 bp
  90% of sequence in fragments shorter than    3048 bp
  95% of sequence in fragments shorter than    3788 bp

# HindIII.StyI (AAGCTT+CCWWGG) double digest, 3973 thousand fragments (860365+3114107 sites), 749 bp average fragment length
   5% of sequence in fragments shorter than     258 bp
  10% of sequence in fragments shorter than     392 bp
  15% of sequence in fragments shorter than     508 bp
  20% of sequence in fragments shorter than     618 bp
  25% of sequence in fragments shorter than     722 bp
  30% of sequence in fragments shorter than     825 bp
  35% of sequence in fragments shorter than     938 bp
  40% of sequence in fragments shorter than    1056 bp
  45% of sequence in fragments shorter than    1180 bp
  50% of sequence in fragments shorter than    1310 bp
  55% of sequence in fragments shorter than    1446 bp
  60% of sequence in fragments shorter than    1601 bp
  65% of sequence in fragments shorter than    1774 bp
  70% of sequence in fragments shorter than    1952 bp
  75% of sequence in fragments shorter than    2179 bp
  80% of sequence in fragments shorter than    2452 bp
  85% of sequence in fragments shorter than    2799 bp
  90% of sequence in fragments shorter than    3302 bp
  95% of sequence in fragments shorter than    4239 bp

# MlyI.PmeI (GAGTC+GTTTAAAC) double digest, 3973 thousand fragments (3931692+42094 sites), 750 bp average fragment length
   5% of sequence in fragments shorter than     261 bp
  10% of sequence in fragments shorter than     388 bp
  15% of sequence in fragments shorter than     503 bp
  20% of sequence in fragments shorter than     612 bp
  25% of sequence in fragments shorter than     719 bp
  30% of sequence in fragments shorter than     826 bp
  35% of sequence in fragments shorter than     934 bp
  40% of sequence in fragments shorter than    1047 bp
  45% of sequence in fragments shorter than    1168 bp
  50% of sequence in fragments shorter than    1299 bp
  55% of sequence in fragments shorter than    1438 bp
  60% of sequence in fragments shorter than    1593 bp
  65% of sequence in fragments shorter than    1762 bp
  70% of sequence in fragments shorter than    1960 bp
  75% of sequence in fragments shorter than    2197 bp
  80% of sequence in fragments shorter than    2495 bp
  85% of sequence in fragments shorter than    2881 bp
  90% of sequence in fragments shorter than    3438 bp
  95% of sequence in fragments shorter than    4489 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# HpaII.PsiI (CCGG+TTATAA) double digest, 3965 thousand fragments (2317722+1647912 sites), 751 bp average fragment length
   5% of sequence in fragments shorter than     244 bp
  10% of sequence in fragments shorter than     403 bp
  15% of sequence in fragments shorter than     543 bp
  20% of sequence in fragments shorter than     674 bp
  25% of sequence in fragments shorter than     802 bp
  30% of sequence in fragments shorter than     931 bp
  35% of sequence in fragments shorter than    1063 bp
  40% of sequence in fragments shorter than    1202 bp
  45% of sequence in fragments shorter than    1348 bp
  50% of sequence in fragments shorter than    1501 bp
  55% of sequence in fragments shorter than    1668 bp
  60% of sequence in fragments shorter than    1851 bp
  65% of sequence in fragments shorter than    2053 bp
  70% of sequence in fragments shorter than    2282 bp
  75% of sequence in fragments shorter than    2557 bp
  80% of sequence in fragments shorter than    2889 bp
  85% of sequence in fragments shorter than    3302 bp
  90% of sequence in fragments shorter than    3924 bp
  95% of sequence in fragments shorter than    5128 bp

# StyI.XbaI (CCWWGG+TCTAGA) double digest, 3964 thousand fragments (3114107+850998 sites), 751 bp average fragment length
   5% of sequence in fragments shorter than     256 bp
  10% of sequence in fragments shorter than     392 bp
  15% of sequence in fragments shorter than     509 bp
  20% of sequence in fragments shorter than     618 bp
  25% of sequence in fragments shorter than     725 bp
  30% of sequence in fragments shorter than     838 bp
  35% of sequence in fragments shorter than     946 bp
  40% of sequence in fragments shorter than    1060 bp
  45% of sequence in fragments shorter than    1186 bp
  50% of sequence in fragments shorter than    1317 bp
  55% of sequence in fragments shorter than    1456 bp
  60% of sequence in fragments shorter than    1613 bp
  65% of sequence in fragments shorter than    1779 bp
  70% of sequence in fragments shorter than    1974 bp
  75% of sequence in fragments shorter than    2190 bp
  80% of sequence in fragments shorter than    2462 bp
  85% of sequence in fragments shorter than    2813 bp
  90% of sequence in fragments shorter than    3307 bp
  95% of sequence in fragments shorter than    4182 bp

# HindIII.NspI (AAGCTT+RCATGY) double digest, 3964 thousand fragments (860365+3104359 sites), 751 bp average fragment length
   5% of sequence in fragments shorter than     260 bp
  10% of sequence in fragments shorter than     400 bp
  15% of sequence in fragments shorter than     520 bp
  20% of sequence in fragments shorter than     632 bp
  25% of sequence in fragments shorter than     743 bp
  30% of sequence in fragments shorter than     854 bp
  35% of sequence in fragments shorter than     966 bp
  40% of sequence in fragments shorter than    1081 bp
  45% of sequence in fragments shorter than    1201 bp
  50% of sequence in fragments shorter than    1329 bp
  55% of sequence in fragments shorter than    1466 bp
  60% of sequence in fragments shorter than    1614 bp
  65% of sequence in fragments shorter than    1777 bp
  70% of sequence in fragments shorter than    1940 bp
  75% of sequence in fragments shorter than    2148 bp
  80% of sequence in fragments shorter than    2406 bp
  85% of sequence in fragments shorter than    2740 bp
  90% of sequence in fragments shorter than    3204 bp
  95% of sequence in fragments shorter than    4061 bp

# MlyI.SalI (GAGTC+GTCGAC) double digest, 3962 thousand fragments (3931692+31396 sites), 752 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     389 bp
  15% of sequence in fragments shorter than     505 bp
  20% of sequence in fragments shorter than     615 bp
  25% of sequence in fragments shorter than     722 bp
  30% of sequence in fragments shorter than     829 bp
  35% of sequence in fragments shorter than     939 bp
  40% of sequence in fragments shorter than    1052 bp
  45% of sequence in fragments shorter than    1174 bp
  50% of sequence in fragments shorter than    1306 bp
  55% of sequence in fragments shorter than    1446 bp
  60% of sequence in fragments shorter than    1603 bp
  65% of sequence in fragments shorter than    1773 bp
  70% of sequence in fragments shorter than    1973 bp
  75% of sequence in fragments shorter than    2213 bp
  80% of sequence in fragments shorter than    2514 bp
  85% of sequence in fragments shorter than    2904 bp
  90% of sequence in fragments shorter than    3467 bp
  95% of sequence in fragments shorter than    4527 bp

# EcoRI.StyI (GAATTC+CCWWGG) double digest, 3961 thousand fragments (847401+3114107 sites), 752 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     390 bp
  15% of sequence in fragments shorter than     509 bp
  20% of sequence in fragments shorter than     624 bp
  25% of sequence in fragments shorter than     728 bp
  30% of sequence in fragments shorter than     834 bp
  35% of sequence in fragments shorter than     943 bp
  40% of sequence in fragments shorter than    1060 bp
  45% of sequence in fragments shorter than    1184 bp
  50% of sequence in fragments shorter than    1316 bp
  55% of sequence in fragments shorter than    1452 bp
  60% of sequence in fragments shorter than    1608 bp
  65% of sequence in fragments shorter than    1780 bp
  70% of sequence in fragments shorter than    1973 bp
  75% of sequence in fragments shorter than    2202 bp
  80% of sequence in fragments shorter than    2465 bp
  85% of sequence in fragments shorter than    2809 bp
  90% of sequence in fragments shorter than    3289 bp
  95% of sequence in fragments shorter than    4146 bp

# BscGI.SmlI (CCCGT+CTYRAG) double digest, 3958 thousand fragments (1231070+2727867 sites), 752 bp average fragment length
   5% of sequence in fragments shorter than     256 bp
  10% of sequence in fragments shorter than     372 bp
  15% of sequence in fragments shorter than     498 bp
  20% of sequence in fragments shorter than     610 bp
  25% of sequence in fragments shorter than     720 bp
  30% of sequence in fragments shorter than     835 bp
  35% of sequence in fragments shorter than     950 bp
  40% of sequence in fragments shorter than    1069 bp
  45% of sequence in fragments shorter than    1197 bp
  50% of sequence in fragments shorter than    1334 bp
  55% of sequence in fragments shorter than    1477 bp
  60% of sequence in fragments shorter than    1639 bp
  65% of sequence in fragments shorter than    1819 bp
  70% of sequence in fragments shorter than    2022 bp
  75% of sequence in fragments shorter than    2254 bp
  80% of sequence in fragments shorter than    2536 bp
  85% of sequence in fragments shorter than    2867 bp
  90% of sequence in fragments shorter than    3363 bp
  95% of sequence in fragments shorter than    4201 bp

# NspI.XbaI (RCATGY+TCTAGA) double digest, 3954 thousand fragments (3104359+850998 sites), 753 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     402 bp
  15% of sequence in fragments shorter than     523 bp
  20% of sequence in fragments shorter than     639 bp
  25% of sequence in fragments shorter than     749 bp
  30% of sequence in fragments shorter than     858 bp
  35% of sequence in fragments shorter than     969 bp
  40% of sequence in fragments shorter than    1084 bp
  45% of sequence in fragments shorter than    1206 bp
  50% of sequence in fragments shorter than    1336 bp
  55% of sequence in fragments shorter than    1463 bp
  60% of sequence in fragments shorter than    1613 bp
  65% of sequence in fragments shorter than    1776 bp
  70% of sequence in fragments shorter than    1964 bp
  75% of sequence in fragments shorter than    2178 bp
  80% of sequence in fragments shorter than    2429 bp
  85% of sequence in fragments shorter than    2762 bp
  90% of sequence in fragments shorter than    3236 bp
  95% of sequence in fragments shorter than    4056 bp

# MluI.MlyI (ACGCGT+GAGTC) double digest, 3952 thousand fragments (21415+3931692 sites), 753 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     389 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     616 bp
  25% of sequence in fragments shorter than     723 bp
  30% of sequence in fragments shorter than     831 bp
  35% of sequence in fragments shorter than     940 bp
  40% of sequence in fragments shorter than    1054 bp
  45% of sequence in fragments shorter than    1176 bp
  50% of sequence in fragments shorter than    1308 bp
  55% of sequence in fragments shorter than    1449 bp
  60% of sequence in fragments shorter than    1606 bp
  65% of sequence in fragments shorter than    1778 bp
  70% of sequence in fragments shorter than    1978 bp
  75% of sequence in fragments shorter than    2219 bp
  80% of sequence in fragments shorter than    2521 bp
  85% of sequence in fragments shorter than    2912 bp
  90% of sequence in fragments shorter than    3477 bp
  95% of sequence in fragments shorter than    4540 bp

# EcoRI.NspI (GAATTC+RCATGY) double digest, 3951 thousand fragments (847401+3104359 sites), 754 bp average fragment length
   5% of sequence in fragments shorter than     264 bp
  10% of sequence in fragments shorter than     396 bp
  15% of sequence in fragments shorter than     519 bp
  20% of sequence in fragments shorter than     636 bp
  25% of sequence in fragments shorter than     740 bp
  30% of sequence in fragments shorter than     852 bp
  35% of sequence in fragments shorter than     965 bp
  40% of sequence in fragments shorter than    1080 bp
  45% of sequence in fragments shorter than    1202 bp
  50% of sequence in fragments shorter than    1330 bp
  55% of sequence in fragments shorter than    1465 bp
  60% of sequence in fragments shorter than    1612 bp
  65% of sequence in fragments shorter than    1777 bp
  70% of sequence in fragments shorter than    1961 bp
  75% of sequence in fragments shorter than    2167 bp
  80% of sequence in fragments shorter than    2414 bp
  85% of sequence in fragments shorter than    2743 bp
  90% of sequence in fragments shorter than    3198 bp
  95% of sequence in fragments shorter than    3970 bp

# BscAI.SexAI (GCATC+ACCWGGT) double digest, 3950 thousand fragments (3652926+297900 sites), 754 bp average fragment length
   5% of sequence in fragments shorter than     256 bp
  10% of sequence in fragments shorter than     397 bp
  15% of sequence in fragments shorter than     523 bp
  20% of sequence in fragments shorter than     635 bp
  25% of sequence in fragments shorter than     745 bp
  30% of sequence in fragments shorter than     858 bp
  35% of sequence in fragments shorter than     976 bp
  40% of sequence in fragments shorter than    1098 bp
  45% of sequence in fragments shorter than    1222 bp
  50% of sequence in fragments shorter than    1354 bp
  55% of sequence in fragments shorter than    1495 bp
  60% of sequence in fragments shorter than    1645 bp
  65% of sequence in fragments shorter than    1819 bp
  70% of sequence in fragments shorter than    2012 bp
  75% of sequence in fragments shorter than    2242 bp
  80% of sequence in fragments shorter than    2520 bp
  85% of sequence in fragments shorter than    2875 bp
  90% of sequence in fragments shorter than    3390 bp
  95% of sequence in fragments shorter than    4322 bp

# MlyI.SgrAI (GAGTC+CRCCGGYG) double digest, 3946 thousand fragments (3931692+15270 sites), 755 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     390 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     617 bp
  25% of sequence in fragments shorter than     724 bp
  30% of sequence in fragments shorter than     832 bp
  35% of sequence in fragments shorter than     942 bp
  40% of sequence in fragments shorter than    1056 bp
  45% of sequence in fragments shorter than    1178 bp
  50% of sequence in fragments shorter than    1311 bp
  55% of sequence in fragments shorter than    1451 bp
  60% of sequence in fragments shorter than    1609 bp
  65% of sequence in fragments shorter than    1781 bp
  70% of sequence in fragments shorter than    1981 bp
  75% of sequence in fragments shorter than    2222 bp
  80% of sequence in fragments shorter than    2525 bp
  85% of sequence in fragments shorter than    2917 bp
  90% of sequence in fragments shorter than    3483 bp
  95% of sequence in fragments shorter than    4549 bp

# MlyI.NruI (GAGTC+TCGCGA) double digest, 3946 thousand fragments (3931692+14872 sites), 755 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     390 bp
  15% of sequence in fragments shorter than     507 bp
  20% of sequence in fragments shorter than     617 bp
  25% of sequence in fragments shorter than     724 bp
  30% of sequence in fragments shorter than     832 bp
  35% of sequence in fragments shorter than     942 bp
  40% of sequence in fragments shorter than    1056 bp
  45% of sequence in fragments shorter than    1178 bp
  50% of sequence in fragments shorter than    1311 bp
  55% of sequence in fragments shorter than    1452 bp
  60% of sequence in fragments shorter than    1609 bp
  65% of sequence in fragments shorter than    1781 bp
  70% of sequence in fragments shorter than    1980 bp
  75% of sequence in fragments shorter than    2222 bp
  80% of sequence in fragments shorter than    2524 bp
  85% of sequence in fragments shorter than    2915 bp
  90% of sequence in fragments shorter than    3481 bp
  95% of sequence in fragments shorter than    4546 bp

# MlyI.PvuI (GAGTC+CGATCG) double digest, 3945 thousand fragments (3931692+14186 sites), 755 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     390 bp
  15% of sequence in fragments shorter than     507 bp
  20% of sequence in fragments shorter than     617 bp
  25% of sequence in fragments shorter than     724 bp
  30% of sequence in fragments shorter than     832 bp
  35% of sequence in fragments shorter than     942 bp
  40% of sequence in fragments shorter than    1056 bp
  45% of sequence in fragments shorter than    1178 bp
  50% of sequence in fragments shorter than    1310 bp
  55% of sequence in fragments shorter than    1451 bp
  60% of sequence in fragments shorter than    1608 bp
  65% of sequence in fragments shorter than    1780 bp
  70% of sequence in fragments shorter than    1978 bp
  75% of sequence in fragments shorter than    2219 bp
  80% of sequence in fragments shorter than    2521 bp
  85% of sequence in fragments shorter than    2910 bp
  90% of sequence in fragments shorter than    3472 bp
  95% of sequence in fragments shorter than    4522 bp

# FseI.MlyI (GGCCGGCC+GAGTC) double digest, 3944 thousand fragments (13662+3931692 sites), 755 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     390 bp
  15% of sequence in fragments shorter than     506 bp
  20% of sequence in fragments shorter than     617 bp
  25% of sequence in fragments shorter than     724 bp
  30% of sequence in fragments shorter than     832 bp
  35% of sequence in fragments shorter than     942 bp
  40% of sequence in fragments shorter than    1056 bp
  45% of sequence in fragments shorter than    1179 bp
  50% of sequence in fragments shorter than    1311 bp
  55% of sequence in fragments shorter than    1452 bp
  60% of sequence in fragments shorter than    1609 bp
  65% of sequence in fragments shorter than    1781 bp
  70% of sequence in fragments shorter than    1982 bp
  75% of sequence in fragments shorter than    2224 bp
  80% of sequence in fragments shorter than    2527 bp
  85% of sequence in fragments shorter than    2919 bp
  90% of sequence in fragments shorter than    3486 bp
  95% of sequence in fragments shorter than    4553 bp

# MlyI.NotI (GAGTC+GCGGCCGC) double digest, 3941 thousand fragments (3931692+9795 sites), 756 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     391 bp
  15% of sequence in fragments shorter than     507 bp
  20% of sequence in fragments shorter than     617 bp
  25% of sequence in fragments shorter than     725 bp
  30% of sequence in fragments shorter than     833 bp
  35% of sequence in fragments shorter than     943 bp
  40% of sequence in fragments shorter than    1057 bp
  45% of sequence in fragments shorter than    1180 bp
  50% of sequence in fragments shorter than    1312 bp
  55% of sequence in fragments shorter than    1453 bp
  60% of sequence in fragments shorter than    1611 bp
  65% of sequence in fragments shorter than    1783 bp
  70% of sequence in fragments shorter than    1984 bp
  75% of sequence in fragments shorter than    2226 bp
  80% of sequence in fragments shorter than    2529 bp
  85% of sequence in fragments shorter than    2921 bp
  90% of sequence in fragments shorter than    3488 bp
  95% of sequence in fragments shorter than    4554 bp

# MlyI.RsrII (GAGTC+CGGWCCG) double digest, 3940 thousand fragments (3931692+9764 sites), 756 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     391 bp
  15% of sequence in fragments shorter than     507 bp
  20% of sequence in fragments shorter than     617 bp
  25% of sequence in fragments shorter than     725 bp
  30% of sequence in fragments shorter than     833 bp
  35% of sequence in fragments shorter than     943 bp
  40% of sequence in fragments shorter than    1057 bp
  45% of sequence in fragments shorter than    1180 bp
  50% of sequence in fragments shorter than    1312 bp
  55% of sequence in fragments shorter than    1453 bp
  60% of sequence in fragments shorter than    1611 bp
  65% of sequence in fragments shorter than    1783 bp
  70% of sequence in fragments shorter than    1983 bp
  75% of sequence in fragments shorter than    2225 bp
  80% of sequence in fragments shorter than    2528 bp
  85% of sequence in fragments shorter than    2920 bp
  90% of sequence in fragments shorter than    3487 bp
  95% of sequence in fragments shorter than    4553 bp

# BscAI.KpnI (GCATC+GGTACC) double digest, 3940 thousand fragments (3652926+287974 sites), 756 bp average fragment length
   5% of sequence in fragments shorter than     257 bp
  10% of sequence in fragments shorter than     400 bp
  15% of sequence in fragments shorter than     527 bp
  20% of sequence in fragments shorter than     640 bp
  25% of sequence in fragments shorter than     751 bp
  30% of sequence in fragments shorter than     866 bp
  35% of sequence in fragments shorter than     985 bp
  40% of sequence in fragments shorter than    1108 bp
  45% of sequence in fragments shorter than    1232 bp
  50% of sequence in fragments shorter than    1367 bp
  55% of sequence in fragments shorter than    1508 bp
  60% of sequence in fragments shorter than    1659 bp
  65% of sequence in fragments shorter than    1835 bp
  70% of sequence in fragments shorter than    2033 bp
  75% of sequence in fragments shorter than    2263 bp
  80% of sequence in fragments shorter than    2547 bp
  85% of sequence in fragments shorter than    2910 bp
  90% of sequence in fragments shorter than    3433 bp
  95% of sequence in fragments shorter than    4391 bp

# BscAI.NheI (GCATC+GCTAGC) double digest, 3939 thousand fragments (3652926+287384 sites), 756 bp average fragment length
   5% of sequence in fragments shorter than     255 bp
  10% of sequence in fragments shorter than     399 bp
  15% of sequence in fragments shorter than     525 bp
  20% of sequence in fragments shorter than     636 bp
  25% of sequence in fragments shorter than     750 bp
  30% of sequence in fragments shorter than     864 bp
  35% of sequence in fragments shorter than     983 bp
  40% of sequence in fragments shorter than    1100 bp
  45% of sequence in fragments shorter than    1226 bp
  50% of sequence in fragments shorter than    1361 bp
  55% of sequence in fragments shorter than    1504 bp
  60% of sequence in fragments shorter than    1660 bp
  65% of sequence in fragments shorter than    1833 bp
  70% of sequence in fragments shorter than    2032 bp
  75% of sequence in fragments shorter than    2260 bp
  80% of sequence in fragments shorter than    2543 bp
  85% of sequence in fragments shorter than    2901 bp
  90% of sequence in fragments shorter than    3414 bp
  95% of sequence in fragments shorter than    4340 bp

# AscI.MlyI (GGCGCGCC+GAGTC) double digest, 3935 thousand fragments (4751+3931692 sites), 757 bp average fragment length
   5% of sequence in fragments shorter than     263 bp
  10% of sequence in fragments shorter than     391 bp
  15% of sequence in fragments shorter than     508 bp
  20% of sequence in fragments shorter than     618 bp
  25% of sequence in fragments shorter than     726 bp
  30% of sequence in fragments shorter than     834 bp
  35% of sequence in fragments shorter than     944 bp
  40% of sequence in fragments shorter than    1058 bp
  45% of sequence in fragments shorter than    1181 bp
  50% of sequence in fragments shorter than    1314 bp
  55% of sequence in fragments shorter than    1455 bp
  60% of sequence in fragments shorter than    1612 bp
  65% of sequence in fragments shorter than    1784 bp
  70% of sequence in fragments shorter than    1985 bp
  75% of sequence in fragments shorter than    2227 bp
  80% of sequence in fragments shorter than    2530 bp
  85% of sequence in fragments shorter than    2922 bp
  90% of sequence in fragments shorter than    3489 bp
  95% of sequence in fragments shorter than    4555 bp

# SfeI.SpeI (CTRYAG+ACTAGT) double digest, 3930 thousand fragments (3531009+400286 sites), 758 bp average fragment length
   5% of sequence in fragments shorter than     268 bp
  10% of sequence in fragments shorter than     400 bp
  15% of sequence in fragments shorter than     511 bp
  20% of sequence in fragments shorter than     623 bp
  25% of sequence in fragments shorter than     726 bp
  30% of sequence in fragments shorter than     831 bp
  35% of sequence in fragments shorter than     938 bp
  40% of sequence in fragments shorter than    1049 bp
  45% of sequence in fragments shorter than    1163 bp
  50% of sequence in fragments shorter than    1286 bp
  55% of sequence in fragments shorter than    1415 bp
  60% of sequence in fragments shorter than    1560 bp
  65% of sequence in fragments shorter than    1718 bp
  70% of sequence in fragments shorter than    1896 bp
  75% of sequence in fragments shorter than    2099 bp
  80% of sequence in fragments shorter than    2324 bp
  85% of sequence in fragments shorter than    2638 bp
  90% of sequence in fragments shorter than    3073 bp
  95% of sequence in fragments shorter than    3837 bp

# HgiCI.SspI (GGYRCC+AATATT) double digest, 3927 thousand fragments (1550877+2377267 sites), 758 bp average fragment length
   5% of sequence in fragments shorter than     261 bp
  10% of sequence in fragments shorter than     396 bp
  15% of sequence in fragments shorter than     515 bp
  20% of sequence in fragments shorter than     627 bp
  25% of sequence in fragments shorter than     735 bp
  30% of sequence in fragments shorter than     846 bp
  35% of sequence in fragments shorter than     958 bp
  40% of sequence in fragments shorter than    1068 bp
  45% of sequence in fragments shorter than    1188 bp
  50% of sequence in fragments shorter than    1309 bp
  55% of sequence in fragments shorter than    1442 bp
  60% of sequence in fragments shorter than    1581 bp
  65% of sequence in fragments shorter than    1742 bp
  70% of sequence in fragments shorter than    1927 bp
  75% of sequence in fragments shorter than    2143 bp
  80% of sequence in fragments shorter than    2403 bp
  85% of sequence in fragments shorter than    2738 bp
  90% of sequence in fragments shorter than    3218 bp
  95% of sequence in fragments shorter than    4125 bp

# BsmI.SspI (GAATGC+AATATT) double digest, 3926 thousand fragments (1549350+2377267 sites), 759 bp average fragment length
   5% of sequence in fragments shorter than     238 bp
  10% of sequence in fragments shorter than     384 bp
  15% of sequence in fragments shorter than     523 bp
  20% of sequence in fragments shorter than     654 bp
  25% of sequence in fragments shorter than     781 bp
  30% of sequence in fragments shorter than     907 bp
  35% of sequence in fragments shorter than    1036 bp
  40% of sequence in fragments shorter than    1172 bp
  45% of sequence in fragments shorter than    1317 bp
  50% of sequence in fragments shorter than    1473 bp
  55% of sequence in fragments shorter than    1641 bp
  60% of sequence in fragments shorter than    1824 bp
  65% of sequence in fragments shorter than    2020 bp
  70% of sequence in fragments shorter than    2253 bp
  75% of sequence in fragments shorter than    2527 bp
  80% of sequence in fragments shorter than    2871 bp
  85% of sequence in fragments shorter than    3324 bp
  90% of sequence in fragments shorter than    3994 bp
  95% of sequence in fragments shorter than    5230 bp

# EsaBC3I.HpaII (TCGA+CCGG) double digest, 3920 thousand fragments (1603346+2317722 sites), 760 bp average fragment length
   5% of sequence in fragments shorter than     233 bp
  10% of sequence in fragments shorter than     411 bp
  15% of sequence in fragments shorter than     569 bp
  20% of sequence in fragments shorter than     726 bp
  25% of sequence in fragments shorter than     884 bp
  30% of sequence in fragments shorter than    1048 bp
  35% of sequence in fragments shorter than    1217 bp
  40% of sequence in fragments shorter than    1391 bp
  45% of sequence in fragments shorter than    1585 bp
  50% of sequence in fragments shorter than    1794 bp
  55% of sequence in fragments shorter than    2027 bp
  60% of sequence in fragments shorter than    2281 bp
  65% of sequence in fragments shorter than    2569 bp
  70% of sequence in fragments shorter than    2898 bp
  75% of sequence in fragments shorter than    3279 bp
  80% of sequence in fragments shorter than    3753 bp
  85% of sequence in fragments shorter than    4359 bp
  90% of sequence in fragments shorter than    5223 bp
  95% of sequence in fragments shorter than    6724 bp

# HpaI.SfeI (GTTAAC+CTRYAG) double digest, 3919 thousand fragments (389418+3531009 sites), 760 bp average fragment length
   5% of sequence in fragments shorter than     268 bp
  10% of sequence in fragments shorter than     401 bp
  15% of sequence in fragments shorter than     514 bp
  20% of sequence in fragments shorter than     627 bp
  25% of sequence in fragments shorter than     731 bp
  30% of sequence in fragments shorter than     841 bp
  35% of sequence in fragments shorter than     952 bp
  40% of sequence in fragments shorter than    1061 bp
  45% of sequence in fragments shorter than    1180 bp
  50% of sequence in fragments shorter than    1305 bp
  55% of sequence in fragments shorter than    1433 bp
  60% of sequence in fragments shorter than    1579 bp
  65% of sequence in fragments shorter than    1738 bp
  70% of sequence in fragments shorter than    1919 bp
  75% of sequence in fragments shorter than    2122 bp
  80% of sequence in fragments shorter than    2351 bp
  85% of sequence in fragments shorter than    2669 bp
  90% of sequence in fragments shorter than    3110 bp
  95% of sequence in fragments shorter than    3895 bp

# SapI.SfeI (GCTCTTC+CTRYAG) double digest, 3907 thousand fragments (377161+3531009 sites), 762 bp average fragment length
   5% of sequence in fragments shorter than     266 bp
  10% of sequence in fragments shorter than     402 bp
  15% of sequence in fragments shorter than     516 bp
  20% of sequence in fragments shorter than     630 bp
  25% of sequence in fragments shorter than     735 bp
  30% of sequence in fragments shorter than     843 bp
  35% of sequence in fragments shorter than     956 bp
  40% of sequence in fragments shorter than    1067 bp
  45% of sequence in fragments shorter than    1190 bp
  50% of sequence in fragments shorter than    1318 bp
  55% of sequence in fragments shorter than    1448 bp
  60% of sequence in fragments shorter than    1595 bp
  65% of sequence in fragments shorter than    1760 bp
  70% of sequence in fragments shorter than    1944 bp
  75% of sequence in fragments shorter than    2139 bp
  80% of sequence in fragments shorter than    2391 bp
  85% of sequence in fragments shorter than    2716 bp
  90% of sequence in fragments shorter than    3169 bp
  95% of sequence in fragments shorter than    3977 bp

# SfeI.SmaI (CTRYAG+CCCGGG) double digest, 3907 thousand fragments (3531009+376940 sites), 762 bp average fragment length
   5% of sequence in fragments shorter than     268 bp
  10% of sequence in fragments shorter than     405 bp
  15% of sequence in fragments shorter than     521 bp
  20% of sequence in fragments shorter than     638 bp
  25% of sequence in fragments shorter than     746 bp
  30% of sequence in fragments shorter than     862 bp
  35% of sequence in fragments shorter than     977 bp
  40% of sequence in fragments shorter than    1095 bp
  45% of sequence in fragments shorter than    1217 bp
  50% of sequence in fragments shorter than    1352 bp
  55% of sequence in fragments shorter than    1490 bp
  60% of sequence in fragments shorter than    1645 bp
  65% of sequence in fragments shorter than    1816 bp
  70% of sequence in fragments shorter than    2010 bp
  75% of sequence in fragments shorter than    2213 bp
  80% of sequence in fragments shorter than    2485 bp
  85% of sequence in fragments shorter than    2832 bp
  90% of sequence in fragments shorter than    3318 bp
  95% of sequence in fragments shorter than    4180 bp

# BamHI.SfeI (GGATCC+CTRYAG) double digest, 3896 thousand fragments (365999+3531009 sites), 764 bp average fragment length
   5% of sequence in fragments shorter than     263 bp
  10% of sequence in fragments shorter than     401 bp
  15% of sequence in fragments shorter than     519 bp
  20% of sequence in fragments shorter than     634 bp
  25% of sequence in fragments shorter than     740 bp
  30% of sequence in fragments shorter than     854 bp
  35% of sequence in fragments shorter than     967 bp
  40% of sequence in fragments shorter than    1081 bp
  45% of sequence in fragments shorter than    1200 bp
  50% of sequence in fragments shorter than    1329 bp
  55% of sequence in fragments shorter than    1459 bp
  60% of sequence in fragments shorter than    1608 bp
  65% of sequence in fragments shorter than    1773 bp
  70% of sequence in fragments shorter than    1961 bp
  75% of sequence in fragments shorter than    2156 bp
  80% of sequence in fragments shorter than    2414 bp
  85% of sequence in fragments shorter than    2745 bp
  90% of sequence in fragments shorter than    3206 bp
  95% of sequence in fragments shorter than    4027 bp

# BglII.StyI (AGATCT+CCWWGG) double digest, 3888 thousand fragments (774732+3114107 sites), 766 bp average fragment length
   5% of sequence in fragments shorter than     264 bp
  10% of sequence in fragments shorter than     400 bp
  15% of sequence in fragments shorter than     518 bp
  20% of sequence in fragments shorter than     631 bp
  25% of sequence in fragments shorter than     741 bp
  30% of sequence in fragments shorter than     846 bp
  35% of sequence in fragments shorter than     962 bp
  40% of sequence in fragments shorter than    1081 bp
  45% of sequence in fragments shorter than    1208 bp
  50% of sequence in fragments shorter than    1342 bp
  55% of sequence in fragments shorter than    1477 bp
  60% of sequence in fragments shorter than    1636 bp
  65% of sequence in fragments shorter than    1814 bp
  70% of sequence in fragments shorter than    2018 bp
  75% of sequence in fragments shorter than    2247 bp
  80% of sequence in fragments shorter than    2535 bp
  85% of sequence in fragments shorter than    2911 bp
  90% of sequence in fragments shorter than    3459 bp
  95% of sequence in fragments shorter than    4528 bp

# BscAI.BsrBI (GCATC+CCGCTC) double digest, 3887 thousand fragments (3652926+235523 sites), 766 bp average fragment length
   5% of sequence in fragments shorter than     260 bp
  10% of sequence in fragments shorter than     404 bp
  15% of sequence in fragments shorter than     532 bp
  20% of sequence in fragments shorter than     647 bp
  25% of sequence in fragments shorter than     762 bp
  30% of sequence in fragments shorter than     875 bp
  35% of sequence in fragments shorter than     997 bp
  40% of sequence in fragments shorter than    1122 bp
  45% of sequence in fragments shorter than    1250 bp
  50% of sequence in fragments shorter than    1385 bp
  55% of sequence in fragments shorter than    1534 bp
  60% of sequence in fragments shorter than    1691 bp
  65% of sequence in fragments shorter than    1872 bp
  70% of sequence in fragments shorter than    2072 bp
  75% of sequence in fragments shorter than    2308 bp
  80% of sequence in fragments shorter than    2596 bp
  85% of sequence in fragments shorter than    2964 bp
  90% of sequence in fragments shorter than    3482 bp
  95% of sequence in fragments shorter than    4431 bp

# BscAI.KasI (GCATC+GGCGCC) double digest, 3882 thousand fragments (3652926+230381 sites), 767 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     403 bp
  15% of sequence in fragments shorter than     529 bp
  20% of sequence in fragments shorter than     642 bp
  25% of sequence in fragments shorter than     755 bp
  30% of sequence in fragments shorter than     868 bp
  35% of sequence in fragments shorter than     986 bp
  40% of sequence in fragments shorter than    1110 bp
  45% of sequence in fragments shorter than    1234 bp
  50% of sequence in fragments shorter than    1368 bp
  55% of sequence in fragments shorter than    1511 bp
  60% of sequence in fragments shorter than    1659 bp
  65% of sequence in fragments shorter than    1834 bp
  70% of sequence in fragments shorter than    2030 bp
  75% of sequence in fragments shorter than    2260 bp
  80% of sequence in fragments shorter than    2541 bp
  85% of sequence in fragments shorter than    2898 bp
  90% of sequence in fragments shorter than    3410 bp
  95% of sequence in fragments shorter than    4345 bp

# BscAI.SwaI (GCATC+ATTTAAAT) double digest, 3879 thousand fragments (3652926+226602 sites), 768 bp average fragment length
   5% of sequence in fragments shorter than     263 bp
  10% of sequence in fragments shorter than     406 bp
  15% of sequence in fragments shorter than     532 bp
  20% of sequence in fragments shorter than     645 bp
  25% of sequence in fragments shorter than     758 bp
  30% of sequence in fragments shorter than     871 bp
  35% of sequence in fragments shorter than     990 bp
  40% of sequence in fragments shorter than    1112 bp
  45% of sequence in fragments shorter than    1235 bp
  50% of sequence in fragments shorter than    1369 bp
  55% of sequence in fragments shorter than    1510 bp
  60% of sequence in fragments shorter than    1658 bp
  65% of sequence in fragments shorter than    1830 bp
  70% of sequence in fragments shorter than    2024 bp
  75% of sequence in fragments shorter than    2250 bp
  80% of sequence in fragments shorter than    2528 bp
  85% of sequence in fragments shorter than    2880 bp
  90% of sequence in fragments shorter than    3387 bp
  95% of sequence in fragments shorter than    4319 bp

# BglII.NspI (AGATCT+RCATGY) double digest, 3878 thousand fragments (774732+3104359 sites), 768 bp average fragment length
   5% of sequence in fragments shorter than     266 bp
  10% of sequence in fragments shorter than     408 bp
  15% of sequence in fragments shorter than     532 bp
  20% of sequence in fragments shorter than     648 bp
  25% of sequence in fragments shorter than     760 bp
  30% of sequence in fragments shorter than     871 bp
  35% of sequence in fragments shorter than     987 bp
  40% of sequence in fragments shorter than    1104 bp
  45% of sequence in fragments shorter than    1226 bp
  50% of sequence in fragments shorter than    1358 bp
  55% of sequence in fragments shorter than    1497 bp
  60% of sequence in fragments shorter than    1648 bp
  65% of sequence in fragments shorter than    1816 bp
  70% of sequence in fragments shorter than    2007 bp
  75% of sequence in fragments shorter than    2227 bp
  80% of sequence in fragments shorter than    2501 bp
  85% of sequence in fragments shorter than    2833 bp
  90% of sequence in fragments shorter than    3332 bp
  95% of sequence in fragments shorter than    4212 bp

# HgiCI.HpaII (GGYRCC+CCGG) double digest, 3868 thousand fragments (1550877+2317722 sites), 770 bp average fragment length
   5% of sequence in fragments shorter than     237 bp
  10% of sequence in fragments shorter than     427 bp
  15% of sequence in fragments shorter than     603 bp
  20% of sequence in fragments shorter than     776 bp
  25% of sequence in fragments shorter than     956 bp
  30% of sequence in fragments shorter than    1137 bp
  35% of sequence in fragments shorter than    1315 bp
  40% of sequence in fragments shorter than    1524 bp
  45% of sequence in fragments shorter than    1738 bp
  50% of sequence in fragments shorter than    1983 bp
  55% of sequence in fragments shorter than    2246 bp
  60% of sequence in fragments shorter than    2549 bp
  65% of sequence in fragments shorter than    2876 bp
  70% of sequence in fragments shorter than    3265 bp
  75% of sequence in fragments shorter than    3731 bp
  80% of sequence in fragments shorter than    4309 bp
  85% of sequence in fragments shorter than    5077 bp
  90% of sequence in fragments shorter than    6224 bp
  95% of sequence in fragments shorter than    8512 bp

# BsmI.HpaII (GAATGC+CCGG) double digest, 3866 thousand fragments (1549350+2317722 sites), 770 bp average fragment length
   5% of sequence in fragments shorter than     219 bp
  10% of sequence in fragments shorter than     397 bp
  15% of sequence in fragments shorter than     566 bp
  20% of sequence in fragments shorter than     726 bp
  25% of sequence in fragments shorter than     885 bp
  30% of sequence in fragments shorter than    1042 bp
  35% of sequence in fragments shorter than    1206 bp
  40% of sequence in fragments shorter than    1380 bp
  45% of sequence in fragments shorter than    1563 bp
  50% of sequence in fragments shorter than    1754 bp
  55% of sequence in fragments shorter than    1966 bp
  60% of sequence in fragments shorter than    2194 bp
  65% of sequence in fragments shorter than    2450 bp
  70% of sequence in fragments shorter than    2742 bp
  75% of sequence in fragments shorter than    3077 bp
  80% of sequence in fragments shorter than    3486 bp
  85% of sequence in fragments shorter than    4006 bp
  90% of sequence in fragments shorter than    4730 bp
  95% of sequence in fragments shorter than    5969 bp

# BstEII.SfeI (GGTNACC+CTRYAG) double digest, 3866 thousand fragments (335865+3531009 sites), 770 bp average fragment length
   5% of sequence in fragments shorter than     269 bp
  10% of sequence in fragments shorter than     406 bp
  15% of sequence in fragments shorter than     522 bp
  20% of sequence in fragments shorter than     637 bp
  25% of sequence in fragments shorter than     744 bp
  30% of sequence in fragments shorter than     856 bp
  35% of sequence in fragments shorter than     965 bp
  40% of sequence in fragments shorter than    1079 bp
  45% of sequence in fragments shorter than    1203 bp
  50% of sequence in fragments shorter than    1333 bp
  55% of sequence in fragments shorter than    1466 bp
  60% of sequence in fragments shorter than    1617 bp
  65% of sequence in fragments shorter than    1785 bp
  70% of sequence in fragments shorter than    1973 bp
  75% of sequence in fragments shorter than    2169 bp
  80% of sequence in fragments shorter than    2430 bp
  85% of sequence in fragments shorter than    2763 bp
  90% of sequence in fragments shorter than    3227 bp
  95% of sequence in fragments shorter than    4061 bp

# NcoI.NspI (CCATGG+RCATGY) double digest, 3863 thousand fragments (759594+3104359 sites), 771 bp average fragment length
   5% of sequence in fragments shorter than     267 bp
  10% of sequence in fragments shorter than     413 bp
  15% of sequence in fragments shorter than     542 bp
  20% of sequence in fragments shorter than     662 bp
  25% of sequence in fragments shorter than     778 bp
  30% of sequence in fragments shorter than     894 bp
  35% of sequence in fragments shorter than    1013 bp
  40% of sequence in fragments shorter than    1135 bp
  45% of sequence in fragments shorter than    1261 bp
  50% of sequence in fragments shorter than    1395 bp
  55% of sequence in fragments shorter than    1542 bp
  60% of sequence in fragments shorter than    1700 bp
  65% of sequence in fragments shorter than    1877 bp
  70% of sequence in fragments shorter than    2077 bp
  75% of sequence in fragments shorter than    2306 bp
  80% of sequence in fragments shorter than    2596 bp
  85% of sequence in fragments shorter than    2964 bp
  90% of sequence in fragments shorter than    3480 bp
  95% of sequence in fragments shorter than    4448 bp

# AflIII.SspI (ACRYGT+AATATT) double digest, 3862 thousand fragments (1485394+2377267 sites), 771 bp average fragment length
   5% of sequence in fragments shorter than     260 bp
  10% of sequence in fragments shorter than     402 bp
  15% of sequence in fragments shorter than     531 bp
  20% of sequence in fragments shorter than     657 bp
  25% of sequence in fragments shorter than     777 bp
  30% of sequence in fragments shorter than     901 bp
  35% of sequence in fragments shorter than    1032 bp
  40% of sequence in fragments shorter than    1166 bp
  45% of sequence in fragments shorter than    1311 bp
  50% of sequence in fragments shorter than    1463 bp
  55% of sequence in fragments shorter than    1633 bp
  60% of sequence in fragments shorter than    1823 bp
  65% of sequence in fragments shorter than    2035 bp
  70% of sequence in fragments shorter than    2280 bp
  75% of sequence in fragments shorter than    2575 bp
  80% of sequence in fragments shorter than    2933 bp
  85% of sequence in fragments shorter than    3404 bp
  90% of sequence in fragments shorter than    4072 bp
  95% of sequence in fragments shorter than    5482 bp

# BclI.StyI (TGATCA+CCWWGG) double digest, 3852 thousand fragments (738785+3114107 sites), 773 bp average fragment length
   5% of sequence in fragments shorter than     266 bp
  10% of sequence in fragments shorter than     403 bp
  15% of sequence in fragments shorter than     521 bp
  20% of sequence in fragments shorter than     634 bp
  25% of sequence in fragments shorter than     746 bp
  30% of sequence in fragments shorter than     855 bp
  35% of sequence in fragments shorter than     970 bp
  40% of sequence in fragments shorter than    1091 bp
  45% of sequence in fragments shorter than    1220 bp
  50% of sequence in fragments shorter than    1355 bp
  55% of sequence in fragments shorter than    1498 bp
  60% of sequence in fragments shorter than    1659 bp
  65% of sequence in fragments shorter than    1839 bp
  70% of sequence in fragments shorter than    2045 bp
  75% of sequence in fragments shorter than    2285 bp
  80% of sequence in fragments shorter than    2558 bp
  85% of sequence in fragments shorter than    2931 bp
  90% of sequence in fragments shorter than    3472 bp
  95% of sequence in fragments shorter than    4514 bp

# AccI.AvaII (GTMKAC+GGWCC) double digest, 3848 thousand fragments (1101063+2747690 sites), 774 bp average fragment length
   5% of sequence in fragments shorter than     259 bp
  10% of sequence in fragments shorter than     398 bp
  15% of sequence in fragments shorter than     530 bp
  20% of sequence in fragments shorter than     657 bp
  25% of sequence in fragments shorter than     779 bp
  30% of sequence in fragments shorter than     902 bp
  35% of sequence in fragments shorter than    1023 bp
  40% of sequence in fragments shorter than    1155 bp
  45% of sequence in fragments shorter than    1293 bp
  50% of sequence in fragments shorter than    1437 bp
  55% of sequence in fragments shorter than    1597 bp
  60% of sequence in fragments shorter than    1755 bp
  65% of sequence in fragments shorter than    1943 bp
  70% of sequence in fragments shorter than    2162 bp
  75% of sequence in fragments shorter than    2415 bp
  80% of sequence in fragments shorter than    2711 bp
  85% of sequence in fragments shorter than    3095 bp
  90% of sequence in fragments shorter than    3642 bp
  95% of sequence in fragments shorter than    4609 bp

# FnuDII.StyI (CGCG+CCWWGG) double digest, 3847 thousand fragments (733940+3114107 sites), 774 bp average fragment length
   5% of sequence in fragments shorter than     259 bp
  10% of sequence in fragments shorter than     412 bp
  15% of sequence in fragments shorter than     547 bp
  20% of sequence in fragments shorter than     676 bp
  25% of sequence in fragments shorter than     797 bp
  30% of sequence in fragments shorter than     931 bp
  35% of sequence in fragments shorter than    1067 bp
  40% of sequence in fragments shorter than    1210 bp
  45% of sequence in fragments shorter than    1362 bp
  50% of sequence in fragments shorter than    1520 bp
  55% of sequence in fragments shorter than    1700 bp
  60% of sequence in fragments shorter than    1897 bp
  65% of sequence in fragments shorter than    2122 bp
  70% of sequence in fragments shorter than    2378 bp
  75% of sequence in fragments shorter than    2680 bp
  80% of sequence in fragments shorter than    3045 bp
  85% of sequence in fragments shorter than    3483 bp
  90% of sequence in fragments shorter than    4183 bp
  95% of sequence in fragments shorter than    5537 bp

# BclI.NspI (TGATCA+RCATGY) double digest, 3842 thousand fragments (738785+3104359 sites), 775 bp average fragment length
   5% of sequence in fragments shorter than     268 bp
  10% of sequence in fragments shorter than     412 bp
  15% of sequence in fragments shorter than     538 bp
  20% of sequence in fragments shorter than     658 bp
  25% of sequence in fragments shorter than     772 bp
  30% of sequence in fragments shorter than     885 bp
  35% of sequence in fragments shorter than    1003 bp
  40% of sequence in fragments shorter than    1122 bp
  45% of sequence in fragments shorter than    1247 bp
  50% of sequence in fragments shorter than    1381 bp
  55% of sequence in fragments shorter than    1524 bp
  60% of sequence in fragments shorter than    1678 bp
  65% of sequence in fragments shorter than    1847 bp
  70% of sequence in fragments shorter than    2030 bp
  75% of sequence in fragments shorter than    2251 bp
  80% of sequence in fragments shorter than    2527 bp
  85% of sequence in fragments shorter than    2876 bp
  90% of sequence in fragments shorter than    3383 bp
  95% of sequence in fragments shorter than    4314 bp

# FnuDII.NspI (CGCG+RCATGY) double digest, 3837 thousand fragments (733940+3104359 sites), 776 bp average fragment length
   5% of sequence in fragments shorter than     269 bp
  10% of sequence in fragments shorter than     426 bp
  15% of sequence in fragments shorter than     564 bp
  20% of sequence in fragments shorter than     693 bp
  25% of sequence in fragments shorter than     817 bp
  30% of sequence in fragments shorter than     942 bp
  35% of sequence in fragments shorter than    1067 bp
  40% of sequence in fragments shorter than    1197 bp
  45% of sequence in fragments shorter than    1334 bp
  50% of sequence in fragments shorter than    1479 bp
  55% of sequence in fragments shorter than    1635 bp
  60% of sequence in fragments shorter than    1803 bp
  65% of sequence in fragments shorter than    1990 bp
  70% of sequence in fragments shorter than    2198 bp
  75% of sequence in fragments shorter than    2448 bp
  80% of sequence in fragments shorter than    2752 bp
  85% of sequence in fragments shorter than    3137 bp
  90% of sequence in fragments shorter than    3679 bp
  95% of sequence in fragments shorter than    4707 bp

# AvaII.PpuMI (GGWCC+RGGWCCY) double digest, 3832 thousand fragments (2747690+1085138 sites), 777 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     552 bp
  15% of sequence in fragments shorter than     750 bp
  20% of sequence in fragments shorter than     933 bp
  25% of sequence in fragments shorter than    1129 bp
  30% of sequence in fragments shorter than    1333 bp
  35% of sequence in fragments shorter than    1542 bp
  40% of sequence in fragments shorter than    1759 bp
  45% of sequence in fragments shorter than    1990 bp
  50% of sequence in fragments shorter than    2241 bp
  55% of sequence in fragments shorter than    2513 bp
  60% of sequence in fragments shorter than    2804 bp
  65% of sequence in fragments shorter than    3140 bp
  70% of sequence in fragments shorter than    3525 bp
  75% of sequence in fragments shorter than    3982 bp
  80% of sequence in fragments shorter than    4542 bp
  85% of sequence in fragments shorter than    5270 bp
  90% of sequence in fragments shorter than    6327 bp
  95% of sequence in fragments shorter than    8258 bp

# AvaII.PvuII (GGWCC+CAGCTG) double digest, 3831 thousand fragments (2747690+1084260 sites), 777 bp average fragment length
   5% of sequence in fragments shorter than     247 bp
  10% of sequence in fragments shorter than     393 bp
  15% of sequence in fragments shorter than     536 bp
  20% of sequence in fragments shorter than     679 bp
  25% of sequence in fragments shorter than     818 bp
  30% of sequence in fragments shorter than     949 bp
  35% of sequence in fragments shorter than    1101 bp
  40% of sequence in fragments shorter than    1262 bp
  45% of sequence in fragments shorter than    1431 bp
  50% of sequence in fragments shorter than    1614 bp
  55% of sequence in fragments shorter than    1808 bp
  60% of sequence in fragments shorter than    2026 bp
  65% of sequence in fragments shorter than    2273 bp
  70% of sequence in fragments shorter than    2555 bp
  75% of sequence in fragments shorter than    2876 bp
  80% of sequence in fragments shorter than    3280 bp
  85% of sequence in fragments shorter than    3804 bp
  90% of sequence in fragments shorter than    4579 bp
  95% of sequence in fragments shorter than    6003 bp

# SexAI.SfeI (ACCWGGT+CTRYAG) double digest, 3828 thousand fragments (297900+3531009 sites), 778 bp average fragment length
   5% of sequence in fragments shorter than     273 bp
  10% of sequence in fragments shorter than     409 bp
  15% of sequence in fragments shorter than     526 bp
  20% of sequence in fragments shorter than     642 bp
  25% of sequence in fragments shorter than     750 bp
  30% of sequence in fragments shorter than     863 bp
  35% of sequence in fragments shorter than     978 bp
  40% of sequence in fragments shorter than    1093 bp
  45% of sequence in fragments shorter than    1213 bp
  50% of sequence in fragments shorter than    1345 bp
  55% of sequence in fragments shorter than    1479 bp
  60% of sequence in fragments shorter than    1630 bp
  65% of sequence in fragments shorter than    1797 bp
  70% of sequence in fragments shorter than    1984 bp
  75% of sequence in fragments shorter than    2181 bp
  80% of sequence in fragments shorter than    2445 bp
  85% of sequence in fragments shorter than    2779 bp
  90% of sequence in fragments shorter than    3251 bp
  95% of sequence in fragments shorter than    4076 bp

# AccI.SmlI (GTMKAC+CTYRAG) double digest, 3828 thousand fragments (1101063+2727867 sites), 778 bp average fragment length
   5% of sequence in fragments shorter than     268 bp
  10% of sequence in fragments shorter than     410 bp
  15% of sequence in fragments shorter than     526 bp
  20% of sequence in fragments shorter than     640 bp
  25% of sequence in fragments shorter than     749 bp
  30% of sequence in fragments shorter than     859 bp
  35% of sequence in fragments shorter than     972 bp
  40% of sequence in fragments shorter than    1085 bp
  45% of sequence in fragments shorter than    1206 bp
  50% of sequence in fragments shorter than    1333 bp
  55% of sequence in fragments shorter than    1465 bp
  60% of sequence in fragments shorter than    1615 bp
  65% of sequence in fragments shorter than    1774 bp
  70% of sequence in fragments shorter than    1950 bp
  75% of sequence in fragments shorter than    2162 bp
  80% of sequence in fragments shorter than    2420 bp
  85% of sequence in fragments shorter than    2724 bp
  90% of sequence in fragments shorter than    3172 bp
  95% of sequence in fragments shorter than    3967 bp

# BsaAI.StyI (YACGTR+CCWWGG) double digest, 3827 thousand fragments (713801+3114107 sites), 778 bp average fragment length
   5% of sequence in fragments shorter than     266 bp
  10% of sequence in fragments shorter than     406 bp
  15% of sequence in fragments shorter than     529 bp
  20% of sequence in fragments shorter than     646 bp
  25% of sequence in fragments shorter than     756 bp
  30% of sequence in fragments shorter than     874 bp
  35% of sequence in fragments shorter than     995 bp
  40% of sequence in fragments shorter than    1118 bp
  45% of sequence in fragments shorter than    1252 bp
  50% of sequence in fragments shorter than    1395 bp
  55% of sequence in fragments shorter than    1550 bp
  60% of sequence in fragments shorter than    1720 bp
  65% of sequence in fragments shorter than    1909 bp
  70% of sequence in fragments shorter than    2123 bp
  75% of sequence in fragments shorter than    2368 bp
  80% of sequence in fragments shorter than    2677 bp
  85% of sequence in fragments shorter than    3069 bp
  90% of sequence in fragments shorter than    3612 bp
  95% of sequence in fragments shorter than    4637 bp

# AclI.BscAI (AACGTT+GCATC) double digest, 3825 thousand fragments (172706+3652926 sites), 779 bp average fragment length
   5% of sequence in fragments shorter than     266 bp
  10% of sequence in fragments shorter than     411 bp
  15% of sequence in fragments shorter than     540 bp
  20% of sequence in fragments shorter than     656 bp
  25% of sequence in fragments shorter than     772 bp
  30% of sequence in fragments shorter than     888 bp
  35% of sequence in fragments shorter than    1010 bp
  40% of sequence in fragments shorter than    1131 bp
  45% of sequence in fragments shorter than    1257 bp
  50% of sequence in fragments shorter than    1390 bp
  55% of sequence in fragments shorter than    1538 bp
  60% of sequence in fragments shorter than    1690 bp
  65% of sequence in fragments shorter than    1867 bp
  70% of sequence in fragments shorter than    2065 bp
  75% of sequence in fragments shorter than    2292 bp
  80% of sequence in fragments shorter than    2574 bp
  85% of sequence in fragments shorter than    2932 bp
  90% of sequence in fragments shorter than    3435 bp
  95% of sequence in fragments shorter than    4335 bp

# BceAI.StyI (ACGGC+CCWWGG) double digest, 3818 thousand fragments (705178+3114107 sites), 780 bp average fragment length
   5% of sequence in fragments shorter than     260 bp
  10% of sequence in fragments shorter than     402 bp
  15% of sequence in fragments shorter than     527 bp
  20% of sequence in fragments shorter than     649 bp
  25% of sequence in fragments shorter than     764 bp
  30% of sequence in fragments shorter than     890 bp
  35% of sequence in fragments shorter than    1019 bp
  40% of sequence in fragments shorter than    1155 bp
  45% of sequence in fragments shorter than    1298 bp
  50% of sequence in fragments shorter than    1449 bp
  55% of sequence in fragments shorter than    1618 bp
  60% of sequence in fragments shorter than    1804 bp
  65% of sequence in fragments shorter than    2015 bp
  70% of sequence in fragments shorter than    2255 bp
  75% of sequence in fragments shorter than    2538 bp
  80% of sequence in fragments shorter than    2877 bp
  85% of sequence in fragments shorter than    3295 bp
  90% of sequence in fragments shorter than    3936 bp
  95% of sequence in fragments shorter than    5164 bp

# KpnI.SfeI (GGTACC+CTRYAG) double digest, 3818 thousand fragments (287974+3531009 sites), 780 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     415 bp
  15% of sequence in fragments shorter than     531 bp
  20% of sequence in fragments shorter than     646 bp
  25% of sequence in fragments shorter than     754 bp
  30% of sequence in fragments shorter than     869 bp
  35% of sequence in fragments shorter than     978 bp
  40% of sequence in fragments shorter than    1094 bp
  45% of sequence in fragments shorter than    1208 bp
  50% of sequence in fragments shorter than    1339 bp
  55% of sequence in fragments shorter than    1472 bp
  60% of sequence in fragments shorter than    1621 bp
  65% of sequence in fragments shorter than    1789 bp
  70% of sequence in fragments shorter than    1976 bp
  75% of sequence in fragments shorter than    2190 bp
  80% of sequence in fragments shorter than    2455 bp
  85% of sequence in fragments shorter than    2788 bp
  90% of sequence in fragments shorter than    3260 bp
  95% of sequence in fragments shorter than    4102 bp

# NheI.SfeI (GCTAGC+CTRYAG) double digest, 3817 thousand fragments (287384+3531009 sites), 780 bp average fragment length
   5% of sequence in fragments shorter than     272 bp
  10% of sequence in fragments shorter than     407 bp
  15% of sequence in fragments shorter than     528 bp
  20% of sequence in fragments shorter than     645 bp
  25% of sequence in fragments shorter than     756 bp
  30% of sequence in fragments shorter than     871 bp
  35% of sequence in fragments shorter than     988 bp
  40% of sequence in fragments shorter than    1103 bp
  45% of sequence in fragments shorter than    1225 bp
  50% of sequence in fragments shorter than    1356 bp
  55% of sequence in fragments shorter than    1489 bp
  60% of sequence in fragments shorter than    1632 bp
  65% of sequence in fragments shorter than    1790 bp
  70% of sequence in fragments shorter than    1975 bp
  75% of sequence in fragments shorter than    2192 bp
  80% of sequence in fragments shorter than    2453 bp
  85% of sequence in fragments shorter than    2788 bp
  90% of sequence in fragments shorter than    3256 bp
  95% of sequence in fragments shorter than    4085 bp

# BsaAI.NspI (YACGTR+RCATGY) double digest, 3817 thousand fragments (713801+3104359 sites), 780 bp average fragment length
   5% of sequence in fragments shorter than     274 bp
  10% of sequence in fragments shorter than     421 bp
  15% of sequence in fragments shorter than     552 bp
  20% of sequence in fragments shorter than     674 bp
  25% of sequence in fragments shorter than     790 bp
  30% of sequence in fragments shorter than     909 bp
  35% of sequence in fragments shorter than    1028 bp
  40% of sequence in fragments shorter than    1147 bp
  45% of sequence in fragments shorter than    1276 bp
  50% of sequence in fragments shorter than    1411 bp
  55% of sequence in fragments shorter than    1559 bp
  60% of sequence in fragments shorter than    1714 bp
  65% of sequence in fragments shorter than    1887 bp
  70% of sequence in fragments shorter than    2085 bp
  75% of sequence in fragments shorter than    2316 bp
  80% of sequence in fragments shorter than    2598 bp
  85% of sequence in fragments shorter than    2945 bp
  90% of sequence in fragments shorter than    3442 bp
  95% of sequence in fragments shorter than    4265 bp

# FauI.SspI (CCCGC+AATATT) double digest, 3816 thousand fragments (1439772+2377267 sites), 780 bp average fragment length
   5% of sequence in fragments shorter than     269 bp
  10% of sequence in fragments shorter than     422 bp
  15% of sequence in fragments shorter than     559 bp
  20% of sequence in fragments shorter than     688 bp
  25% of sequence in fragments shorter than     814 bp
  30% of sequence in fragments shorter than     943 bp
  35% of sequence in fragments shorter than    1073 bp
  40% of sequence in fragments shorter than    1208 bp
  45% of sequence in fragments shorter than    1349 bp
  50% of sequence in fragments shorter than    1492 bp
  55% of sequence in fragments shorter than    1653 bp
  60% of sequence in fragments shorter than    1830 bp
  65% of sequence in fragments shorter than    2027 bp
  70% of sequence in fragments shorter than    2253 bp
  75% of sequence in fragments shorter than    2515 bp
  80% of sequence in fragments shorter than    2829 bp
  85% of sequence in fragments shorter than    3244 bp
  90% of sequence in fragments shorter than    3840 bp
  95% of sequence in fragments shorter than    4871 bp

# BspMI.SspI (ACCTGC+AATATT) double digest, 3814 thousand fragments (1438129+2377267 sites), 781 bp average fragment length
   5% of sequence in fragments shorter than     273 bp
  10% of sequence in fragments shorter than     407 bp
  15% of sequence in fragments shorter than     533 bp
  20% of sequence in fragments shorter than     653 bp
  25% of sequence in fragments shorter than     762 bp
  30% of sequence in fragments shorter than     874 bp
  35% of sequence in fragments shorter than     987 bp
  40% of sequence in fragments shorter than    1105 bp
  45% of sequence in fragments shorter than    1233 bp
  50% of sequence in fragments shorter than    1369 bp
  55% of sequence in fragments shorter than    1510 bp
  60% of sequence in fragments shorter than    1665 bp
  65% of sequence in fragments shorter than    1836 bp
  70% of sequence in fragments shorter than    2035 bp
  75% of sequence in fragments shorter than    2266 bp
  80% of sequence in fragments shorter than    2547 bp
  85% of sequence in fragments shorter than    2901 bp
  90% of sequence in fragments shorter than    3356 bp
  95% of sequence in fragments shorter than    4192 bp

# PpuMI.SmlI (RGGWCCY+CTYRAG) double digest, 3812 thousand fragments (1085138+2727867 sites), 781 bp average fragment length
   5% of sequence in fragments shorter than     262 bp
  10% of sequence in fragments shorter than     405 bp
  15% of sequence in fragments shorter than     532 bp
  20% of sequence in fragments shorter than     650 bp
  25% of sequence in fragments shorter than     764 bp
  30% of sequence in fragments shorter than     881 bp
  35% of sequence in fragments shorter than    1001 bp
  40% of sequence in fragments shorter than    1124 bp
  45% of sequence in fragments shorter than    1253 bp
  50% of sequence in fragments shorter than    1392 bp
  55% of sequence in fragments shorter than    1543 bp
  60% of sequence in fragments shorter than    1710 bp
  65% of sequence in fragments shorter than    1893 bp
  70% of sequence in fragments shorter than    2096 bp
  75% of sequence in fragments shorter than    2341 bp
  80% of sequence in fragments shorter than    2636 bp
  85% of sequence in fragments shorter than    2987 bp
  90% of sequence in fragments shorter than    3533 bp
  95% of sequence in fragments shorter than    4579 bp

# BscAI.PacI (GCATC+TTAATTAA) double digest, 3811 thousand fragments (3652926+159455 sites), 781 bp average fragment length
   5% of sequence in fragments shorter than     267 bp
  10% of sequence in fragments shorter than     414 bp
  15% of sequence in fragments shorter than     543 bp
  20% of sequence in fragments shorter than     659 bp
  25% of sequence in fragments shorter than     774 bp
  30% of sequence in fragments shorter than     891 bp
  35% of sequence in fragments shorter than    1012 bp
  40% of sequence in fragments shorter than    1137 bp
  45% of sequence in fragments shorter than    1263 bp
  50% of sequence in fragments shorter than    1396 bp
  55% of sequence in fragments shorter than    1545 bp
  60% of sequence in fragments shorter than    1697 bp
  65% of sequence in fragments shorter than    1875 bp
  70% of sequence in fragments shorter than    2073 bp
  75% of sequence in fragments shorter than    2305 bp
  80% of sequence in fragments shorter than    2589 bp
  85% of sequence in fragments shorter than    2953 bp
  90% of sequence in fragments shorter than    3472 bp
  95% of sequence in fragments shorter than    4440 bp

# PvuII.SmlI (CAGCTG+CTYRAG) double digest, 3811 thousand fragments (1084260+2727867 sites), 781 bp average fragment length
   5% of sequence in fragments shorter than     263 bp
  10% of sequence in fragments shorter than     406 bp
  15% of sequence in fragments shorter than     529 bp
  20% of sequence in fragments shorter than     646 bp
  25% of sequence in fragments shorter than     757 bp
  30% of sequence in fragments shorter than     872 bp
  35% of sequence in fragments shorter than     989 bp
  40% of sequence in fragments shorter than    1110 bp
  45% of sequence in fragments shorter than    1237 bp
  50% of sequence in fragments shorter than    1373 bp
  55% of sequence in fragments shorter than    1519 bp
  60% of sequence in fragments shorter than    1683 bp
  65% of sequence in fragments shorter than    1863 bp
  70% of sequence in fragments shorter than    2064 bp
  75% of sequence in fragments shorter than    2297 bp
  80% of sequence in fragments shorter than    2581 bp
  85% of sequence in fragments shorter than    2922 bp
  90% of sequence in fragments shorter than    3443 bp
  95% of sequence in fragments shorter than    4426 bp

# BscAI.Hpy99I (GCATC+CGWCG) double digest, 3810 thousand fragments (3652926+157917 sites), 782 bp average fragment length
   5% of sequence in fragments shorter than     265 bp
  10% of sequence in fragments shorter than     413 bp
  15% of sequence in fragments shorter than     544 bp
  20% of sequence in fragments shorter than     661 bp
  25% of sequence in fragments shorter than     779 bp
  30% of sequence in fragments shorter than     897 bp
  35% of sequence in fragments shorter than    1021 bp
  40% of sequence in fragments shorter than    1148 bp
  45% of sequence in fragments shorter than    1277 bp
  50% of sequence in fragments shorter than    1414 bp
  55% of sequence in fragments shorter than    1564 bp
  60% of sequence in fragments shorter than    1722 bp
  65% of sequence in fragments shorter than    1905 bp
  70% of sequence in fragments shorter than    2109 bp
  75% of sequence in fragments shorter than    2349 bp
  80% of sequence in fragments shorter than    2641 bp
  85% of sequence in fragments shorter than    3015 bp
  90% of sequence in fragments shorter than    3546 bp
  95% of sequence in fragments shorter than    4527 bp

# BciVI.StyI (GTATCC+CCWWGG) double digest, 3809 thousand fragments (696093+3114107 sites), 782 bp average fragment length
   5% of sequence in fragments shorter than     268 bp
  10% of sequence in fragments shorter than     411 bp
  15% of sequence in fragments shorter than     534 bp
  20% of sequence in fragments shorter than     654 bp
  25% of sequence in fragments shorter than     763 bp
  30% of sequence in fragments shorter than     882 bp
  35% of sequence in fragments shorter than    1003 bp
  40% of sequence in fragments shorter than    1129 bp
  45% of sequence in fragments shorter than    1260 bp
  50% of sequence in fragments shorter than    1394 bp
  55% of sequence in fragments shorter than    1546 bp
  60% of sequence in fragments shorter than    1711 bp
  65% of sequence in fragments shorter than    1891 bp
  70% of sequence in fragments shorter than    2100 bp
  75% of sequence in fragments shorter than    2347 bp
  80% of sequence in fragments shorter than    2643 bp
  85% of sequence in fragments shorter than    3025 bp
  90% of sequence in fragments shorter than    3579 bp
  95% of sequence in fragments shorter than    4623 bp

# BceAI.NspI (ACGGC+RCATGY) double digest, 3809 thousand fragments (705178+3104359 sites), 782 bp average fragment length
   5% of sequence in fragments shorter than     271 bp
  10% of sequence in fragments shorter than     416 bp
  15% of sequence in fragments shorter than     545 bp
  20% of sequence in fragments shorter than     666 bp
  25% of sequence in fragments shorter than     782 bp
  30% of sequence in fragments shorter than     899 bp
  35% of sequence in fragments shorter than    1019 bp
  40% of sequence in fragments shorter than    1140 bp
  45% of sequence in fragments shorter than    1269 bp
  50% of sequence in fragments shorter than    1405 bp
  55% of sequence in fragments shorter than    1552 bp
  60% of sequence in fragments shorter than    1711 bp
  65% of sequence in fragments shorter than    1888 bp
  70% of sequence in fragments shorter than    2086 bp
  75% of sequence in fragments shorter than    2319 bp
  80% of sequence in fragments shorter than    2608 bp
  85% of sequence in fragments shorter than    2972 bp
  90% of sequence in fragments shorter than    3482 bp
  95% of sequence in fragments shorter than    4414 bp

# AflIII.HpaII (ACRYGT+CCGG) double digest, 3802 thousand fragments (1485394+2317722 sites), 783 bp average fragment length
   5% of sequence in fragments shorter than     251 bp
  10% of sequence in fragments shorter than     422 bp
  15% of sequence in fragments shorter than     578 bp
  20% of sequence in fragments shorter than     728 bp
  25% of sequence in fragments shorter than     876 bp
  30% of sequence in fragments shorter than    1026 bp
  35% of sequence in fragments shorter than    1181 bp
  40% of sequence in fragments shorter than    1342 bp
  45% of sequence in fragments shorter than    1513 bp
  50% of sequence in fragments shorter than    1693 bp
  55% of sequence in fragments shorter than    1889 bp
  60% of sequence in fragments shorter than    2091 bp
  65% of sequence in fragments shorter than    2332 bp
  70% of sequence in fragments shorter than    2609 bp
  75% of sequence in fragments shorter than    2928 bp
  80% of sequence in fragments shorter than    3311 bp
  85% of sequence in fragments shorter than    3809 bp
  90% of sequence in fragments shorter than    4528 bp
  95% of sequence in fragments shorter than    5819 bp

# BciVI.NspI (GTATCC+RCATGY) double digest, 3799 thousand fragments (696093+3104359 sites), 784 bp average fragment length
   5% of sequence in fragments shorter than     273 bp
  10% of sequence in fragments shorter than     419 bp
  15% of sequence in fragments shorter than     548 bp
  20% of sequence in fragments shorter than     670 bp
  25% of sequence in fragments shorter than     786 bp
  30% of sequence in fragments shorter than     903 bp
  35% of sequence in fragments shorter than    1020 bp
  40% of sequence in fragments shorter than    1141 bp
  45% of sequence in fragments shorter than    1262 bp
  50% of sequence in fragments shorter than    1395 bp
  55% of sequence in fragments shorter than    1535 bp
  60% of sequence in fragments shorter than    1689 bp
  65% of sequence in fragments shorter than    1857 bp
  70% of sequence in fragments shorter than    2053 bp
  75% of sequence in fragments shorter than    2274 bp
  80% of sequence in fragments shorter than    2548 bp
  85% of sequence in fragments shorter than    2895 bp
  90% of sequence in fragments shorter than    3390 bp
  95% of sequence in fragments shorter than    4303 bp

# BscAI.SnaBI (GCATC+TACGTA) double digest, 3784 thousand fragments (3652926+132299 sites), 787 bp average fragment length
   5% of sequence in fragments shorter than     269 bp
  10% of sequence in fragments shorter than     417 bp
  15% of sequence in fragments shorter than     547 bp
  20% of sequence in fragments shorter than     664 bp
  25% of sequence in fragments shorter than     780 bp
  30% of sequence in fragments shorter than     898 bp
  35% of sequence in fragments shorter than    1021 bp
  40% of sequence in fragments shorter than    1147 bp
  45% of sequence in fragments shorter than    1274 bp
  50% of sequence in fragments shorter than    1408 bp
  55% of sequence in fragments shorter than    1556 bp
  60% of sequence in fragments shorter than    1711 bp
  65% of sequence in fragments shorter than    1892 bp
  70% of sequence in fragments shorter than    2092 bp
  75% of sequence in fragments shorter than    2327 bp
  80% of sequence in fragments shorter than    2614 bp
  85% of sequence in fragments shorter than    2980 bp
  90% of sequence in fragments shorter than    3503 bp
  95% of sequence in fragments shorter than    4468 bp

# BscAI.NaeI (GCATC+GCCGGC) double digest, 3778 thousand fragments (3652926+125692 sites), 788 bp average fragment length
   5% of sequence in fragments shorter than     268 bp
  10% of sequence in fragments shorter than     416 bp
  15% of sequence in fragments shorter than     548 bp
  20% of sequence in fragments shorter than     666 bp
  25% of sequence in fragments shorter than     784 bp
  30% of sequence in fragments shorter than     904 bp
  35% of sequence in fragments shorter than    1029 bp
  40% of sequence in fragments shorter than    1157 bp
  45% of sequence in fragments shorter than    1288 bp
  50% of sequence in fragments shorter than    1426 bp
  55% of sequence in fragments shorter than    1576 bp
  60% of sequence in fragments shorter than    1736 bp
  65% of sequence in fragments shorter than    1920 bp
  70% of sequence in fragments shorter than    2127 bp
  75% of sequence in fragments shorter than    2369 bp
  80% of sequence in fragments shorter than    2665 bp
  85% of sequence in fragments shorter than    3043 bp
  90% of sequence in fragments shorter than    3584 bp
  95% of sequence in fragments shorter than    4585 bp

# BscAI.XhoI (GCATC+CTCGAG) double digest, 3773 thousand fragments (3652926+121308 sites), 789 bp average fragment length
   5% of sequence in fragments shorter than     269 bp
  10% of sequence in fragments shorter than     417 bp
  15% of sequence in fragments shorter than     546 bp
  20% of sequence in fragments shorter than     664 bp
  25% of sequence in fragments shorter than     782 bp
  30% of sequence in fragments shorter than     901 bp
  35% of sequence in fragments shorter than    1025 bp
  40% of sequence in fragments shorter than    1152 bp
  45% of sequence in fragments shorter than    1281 bp
  50% of sequence in fragments shorter than    1418 bp
  55% of sequence in fragments shorter than    1567 bp
  60% of sequence in fragments shorter than    1724 bp
  65% of sequence in fragments shorter than    1906 bp
  70% of sequence in fragments shorter than    2111 bp
  75% of sequence in fragments shorter than    2350 bp
  80% of sequence in fragments shorter than    2642 bp
  85% of sequence in fragments shorter than    3015 bp
  90% of sequence in fragments shorter than    3550 bp
  95% of sequence in fragments shorter than    4544 bp

# BsrBI.SfeI (CCGCTC+CTRYAG) double digest, 3766 thousand fragments (235523+3531009 sites), 791 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     417 bp
  15% of sequence in fragments shorter than     536 bp
  20% of sequence in fragments shorter than     655 bp
  25% of sequence in fragments shorter than     767 bp
  30% of sequence in fragments shorter than     883 bp
  35% of sequence in fragments shorter than    1001 bp
  40% of sequence in fragments shorter than    1117 bp
  45% of sequence in fragments shorter than    1245 bp
  50% of sequence in fragments shorter than    1379 bp
  55% of sequence in fragments shorter than    1520 bp
  60% of sequence in fragments shorter than    1678 bp
  65% of sequence in fragments shorter than    1850 bp
  70% of sequence in fragments shorter than    2038 bp
  75% of sequence in fragments shorter than    2249 bp
  80% of sequence in fragments shorter than    2524 bp
  85% of sequence in fragments shorter than    2870 bp
  90% of sequence in fragments shorter than    3359 bp
  95% of sequence in fragments shorter than    4215 bp

# KasI.SfeI (GGCGCC+CTRYAG) double digest, 3760 thousand fragments (230381+3531009 sites), 792 bp average fragment length
   5% of sequence in fragments shorter than     281 bp
  10% of sequence in fragments shorter than     423 bp
  15% of sequence in fragments shorter than     545 bp
  20% of sequence in fragments shorter than     663 bp
  25% of sequence in fragments shorter than     778 bp
  30% of sequence in fragments shorter than     896 bp
  35% of sequence in fragments shorter than    1016 bp
  40% of sequence in fragments shorter than    1133 bp
  45% of sequence in fragments shorter than    1261 bp
  50% of sequence in fragments shorter than    1393 bp
  55% of sequence in fragments shorter than    1540 bp
  60% of sequence in fragments shorter than    1698 bp
  65% of sequence in fragments shorter than    1873 bp
  70% of sequence in fragments shorter than    2070 bp
  75% of sequence in fragments shorter than    2276 bp
  80% of sequence in fragments shorter than    2554 bp
  85% of sequence in fragments shorter than    2902 bp
  90% of sequence in fragments shorter than    3399 bp
  95% of sequence in fragments shorter than    4266 bp

# SfeI.SwaI (CTRYAG+ATTTAAAT) double digest, 3757 thousand fragments (3531009+226602 sites), 793 bp average fragment length
   5% of sequence in fragments shorter than     278 bp
  10% of sequence in fragments shorter than     418 bp
  15% of sequence in fragments shorter than     536 bp
  20% of sequence in fragments shorter than     654 bp
  25% of sequence in fragments shorter than     763 bp
  30% of sequence in fragments shorter than     877 bp
  35% of sequence in fragments shorter than     992 bp
  40% of sequence in fragments shorter than    1104 bp
  45% of sequence in fragments shorter than    1228 bp
  50% of sequence in fragments shorter than    1360 bp
  55% of sequence in fragments shorter than    1493 bp
  60% of sequence in fragments shorter than    1644 bp
  65% of sequence in fragments shorter than    1810 bp
  70% of sequence in fragments shorter than    1995 bp
  75% of sequence in fragments shorter than    2190 bp
  80% of sequence in fragments shorter than    2450 bp
  85% of sequence in fragments shorter than    2779 bp
  90% of sequence in fragments shorter than    3239 bp
  95% of sequence in fragments shorter than    4054 bp

# FauI.HpaII (CCCGC+CCGG) double digest, 3757 thousand fragments (1439772+2317722 sites), 793 bp average fragment length
   5% of sequence in fragments shorter than     239 bp
  10% of sequence in fragments shorter than     470 bp
  15% of sequence in fragments shorter than     690 bp
  20% of sequence in fragments shorter than     914 bp
  25% of sequence in fragments shorter than    1146 bp
  30% of sequence in fragments shorter than    1386 bp
  35% of sequence in fragments shorter than    1654 bp
  40% of sequence in fragments shorter than    1938 bp
  45% of sequence in fragments shorter than    2247 bp
  50% of sequence in fragments shorter than    2596 bp
  55% of sequence in fragments shorter than    2982 bp
  60% of sequence in fragments shorter than    3417 bp
  65% of sequence in fragments shorter than    3912 bp
  70% of sequence in fragments shorter than    4480 bp
  75% of sequence in fragments shorter than    5173 bp
  80% of sequence in fragments shorter than    6009 bp
  85% of sequence in fragments shorter than    7129 bp
  90% of sequence in fragments shorter than    8764 bp
  95% of sequence in fragments shorter than   11789 bp

# AflII.StyI (CTTAAG+CCWWGG) double digest, 3756 thousand fragments (643284+3114107 sites), 793 bp average fragment length
   5% of sequence in fragments shorter than     271 bp
  10% of sequence in fragments shorter than     413 bp
  15% of sequence in fragments shorter than     535 bp
  20% of sequence in fragments shorter than     653 bp
  25% of sequence in fragments shorter than     763 bp
  30% of sequence in fragments shorter than     881 bp
  35% of sequence in fragments shorter than    1003 bp
  40% of sequence in fragments shorter than    1128 bp
  45% of sequence in fragments shorter than    1261 bp
  50% of sequence in fragments shorter than    1398 bp
  55% of sequence in fragments shorter than    1550 bp
  60% of sequence in fragments shorter than    1717 bp
  65% of sequence in fragments shorter than    1904 bp
  70% of sequence in fragments shorter than    2119 bp
  75% of sequence in fragments shorter than    2371 bp
  80% of sequence in fragments shorter than    2670 bp
  85% of sequence in fragments shorter than    3032 bp
  90% of sequence in fragments shorter than    3598 bp
  95% of sequence in fragments shorter than    4690 bp

# BspMI.HpaII (ACCTGC+CCGG) double digest, 3755 thousand fragments (1438129+2317722 sites), 793 bp average fragment length
   5% of sequence in fragments shorter than     242 bp
  10% of sequence in fragments shorter than     419 bp
  15% of sequence in fragments shorter than     587 bp
  20% of sequence in fragments shorter than     750 bp
  25% of sequence in fragments shorter than     906 bp
  30% of sequence in fragments shorter than    1078 bp
  35% of sequence in fragments shorter than    1256 bp
  40% of sequence in fragments shorter than    1442 bp
  45% of sequence in fragments shorter than    1650 bp
  50% of sequence in fragments shorter than    1875 bp
  55% of sequence in fragments shorter than    2105 bp
  60% of sequence in fragments shorter than    2374 bp
  65% of sequence in fragments shorter than    2671 bp
  70% of sequence in fragments shorter than    3017 bp
  75% of sequence in fragments shorter than    3400 bp
  80% of sequence in fragments shorter than    3901 bp
  85% of sequence in fragments shorter than    4553 bp
  90% of sequence in fragments shorter than    5466 bp
  95% of sequence in fragments shorter than    7078 bp

# AvaI.AvaII (CYCGRG+GGWCC) double digest, 3752 thousand fragments (1005714+2747690 sites), 794 bp average fragment length
   5% of sequence in fragments shorter than     260 bp
  10% of sequence in fragments shorter than     404 bp
  15% of sequence in fragments shorter than     549 bp
  20% of sequence in fragments shorter than     691 bp
  25% of sequence in fragments shorter than     836 bp
  30% of sequence in fragments shorter than     973 bp
  35% of sequence in fragments shorter than    1127 bp
  40% of sequence in fragments shorter than    1292 bp
  45% of sequence in fragments shorter than    1466 bp
  50% of sequence in fragments shorter than    1659 bp
  55% of sequence in fragments shorter than    1863 bp
  60% of sequence in fragments shorter than    2089 bp
  65% of sequence in fragments shorter than    2347 bp
  70% of sequence in fragments shorter than    2644 bp
  75% of sequence in fragments shorter than    2994 bp
  80% of sequence in fragments shorter than    3427 bp
  85% of sequence in fragments shorter than    3992 bp
  90% of sequence in fragments shorter than    4822 bp
  95% of sequence in fragments shorter than    6389 bp

# AflII.NspI (CTTAAG+RCATGY) double digest, 3747 thousand fragments (643284+3104359 sites), 795 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     423 bp
  15% of sequence in fragments shorter than     552 bp
  20% of sequence in fragments shorter than     674 bp
  25% of sequence in fragments shorter than     789 bp
  30% of sequence in fragments shorter than     907 bp
  35% of sequence in fragments shorter than    1027 bp
  40% of sequence in fragments shorter than    1149 bp
  45% of sequence in fragments shorter than    1278 bp
  50% of sequence in fragments shorter than    1414 bp
  55% of sequence in fragments shorter than    1551 bp
  60% of sequence in fragments shorter than    1708 bp
  65% of sequence in fragments shorter than    1885 bp
  70% of sequence in fragments shorter than    2082 bp
  75% of sequence in fragments shorter than    2304 bp
  80% of sequence in fragments shorter than    2590 bp
  85% of sequence in fragments shorter than    2954 bp
  90% of sequence in fragments shorter than    3473 bp
  95% of sequence in fragments shorter than    4460 bp

# HaeII.StyI (RGCGCY+CCWWGG) double digest, 3743 thousand fragments (630101+3114107 sites), 795 bp average fragment length
   5% of sequence in fragments shorter than     267 bp
  10% of sequence in fragments shorter than     405 bp
  15% of sequence in fragments shorter than     535 bp
  20% of sequence in fragments shorter than     663 bp
  25% of sequence in fragments shorter than     771 bp
  30% of sequence in fragments shorter than     900 bp
  35% of sequence in fragments shorter than    1033 bp
  40% of sequence in fragments shorter than    1170 bp
  45% of sequence in fragments shorter than    1317 bp
  50% of sequence in fragments shorter than    1474 bp
  55% of sequence in fragments shorter than    1645 bp
  60% of sequence in fragments shorter than    1834 bp
  65% of sequence in fragments shorter than    2048 bp
  70% of sequence in fragments shorter than    2283 bp
  75% of sequence in fragments shorter than    2562 bp
  80% of sequence in fragments shorter than    2899 bp
  85% of sequence in fragments shorter than    3309 bp
  90% of sequence in fragments shorter than    3917 bp
  95% of sequence in fragments shorter than    4976 bp

# BscAI.ClaI (GCATC+ATCGAT) double digest, 3738 thousand fragments (3652926+86477 sites), 797 bp average fragment length
   5% of sequence in fragments shorter than     272 bp
  10% of sequence in fragments shorter than     423 bp
  15% of sequence in fragments shorter than     555 bp
  20% of sequence in fragments shorter than     674 bp
  25% of sequence in fragments shorter than     791 bp
  30% of sequence in fragments shorter than     912 bp
  35% of sequence in fragments shorter than    1037 bp
  40% of sequence in fragments shorter than    1165 bp
  45% of sequence in fragments shorter than    1296 bp
  50% of sequence in fragments shorter than    1434 bp
  55% of sequence in fragments shorter than    1584 bp
  60% of sequence in fragments shorter than    1743 bp
  65% of sequence in fragments shorter than    1926 bp
  70% of sequence in fragments shorter than    2133 bp
  75% of sequence in fragments shorter than    2374 bp
  80% of sequence in fragments shorter than    2668 bp
  85% of sequence in fragments shorter than    3044 bp
  90% of sequence in fragments shorter than    3584 bp
  95% of sequence in fragments shorter than    4579 bp

# HaeII.NspI (RGCGCY+RCATGY) double digest, 3733 thousand fragments (630101+3104359 sites), 798 bp average fragment length
   5% of sequence in fragments shorter than     280 bp
  10% of sequence in fragments shorter than     418 bp
  15% of sequence in fragments shorter than     551 bp
  20% of sequence in fragments shorter than     676 bp
  25% of sequence in fragments shorter than     786 bp
  30% of sequence in fragments shorter than     905 bp
  35% of sequence in fragments shorter than    1028 bp
  40% of sequence in fragments shorter than    1151 bp
  45% of sequence in fragments shorter than    1281 bp
  50% of sequence in fragments shorter than    1418 bp
  55% of sequence in fragments shorter than    1565 bp
  60% of sequence in fragments shorter than    1725 bp
  65% of sequence in fragments shorter than    1902 bp
  70% of sequence in fragments shorter than    2102 bp
  75% of sequence in fragments shorter than    2335 bp
  80% of sequence in fragments shorter than    2619 bp
  85% of sequence in fragments shorter than    2973 bp
  90% of sequence in fragments shorter than    3466 bp
  95% of sequence in fragments shorter than    4257 bp

# AvaII.BspHI (GGWCC+TCATGA) double digest, 3725 thousand fragments (2747690+978290 sites), 799 bp average fragment length
   5% of sequence in fragments shorter than     268 bp
  10% of sequence in fragments shorter than     414 bp
  15% of sequence in fragments shorter than     550 bp
  20% of sequence in fragments shorter than     682 bp
  25% of sequence in fragments shorter than     811 bp
  30% of sequence in fragments shorter than     932 bp
  35% of sequence in fragments shorter than    1066 bp
  40% of sequence in fragments shorter than    1209 bp
  45% of sequence in fragments shorter than    1358 bp
  50% of sequence in fragments shorter than    1515 bp
  55% of sequence in fragments shorter than    1687 bp
  60% of sequence in fragments shorter than    1867 bp
  65% of sequence in fragments shorter than    2074 bp
  70% of sequence in fragments shorter than    2309 bp
  75% of sequence in fragments shorter than    2585 bp
  80% of sequence in fragments shorter than    2908 bp
  85% of sequence in fragments shorter than    3337 bp
  90% of sequence in fragments shorter than    3942 bp
  95% of sequence in fragments shorter than    5060 bp

# AatII.BscAI (GACGTC+GCATC) double digest, 3723 thousand fragments (71089+3652926 sites), 800 bp average fragment length
   5% of sequence in fragments shorter than     272 bp
  10% of sequence in fragments shorter than     423 bp
  15% of sequence in fragments shorter than     555 bp
  20% of sequence in fragments shorter than     675 bp
  25% of sequence in fragments shorter than     793 bp
  30% of sequence in fragments shorter than     914 bp
  35% of sequence in fragments shorter than    1039 bp
  40% of sequence in fragments shorter than    1168 bp
  45% of sequence in fragments shorter than    1300 bp
  50% of sequence in fragments shorter than    1438 bp
  55% of sequence in fragments shorter than    1589 bp
  60% of sequence in fragments shorter than    1750 bp
  65% of sequence in fragments shorter than    1934 bp
  70% of sequence in fragments shorter than    2142 bp
  75% of sequence in fragments shorter than    2385 bp
  80% of sequence in fragments shorter than    2682 bp
  85% of sequence in fragments shorter than    3062 bp
  90% of sequence in fragments shorter than    3605 bp
  95% of sequence in fragments shorter than    4612 bp

# BscAI.SacII (GCATC+CCGCGG) double digest, 3719 thousand fragments (3652926+66901 sites), 801 bp average fragment length
   5% of sequence in fragments shorter than     272 bp
  10% of sequence in fragments shorter than     423 bp
  15% of sequence in fragments shorter than     557 bp
  20% of sequence in fragments shorter than     677 bp
  25% of sequence in fragments shorter than     795 bp
  30% of sequence in fragments shorter than     918 bp
  35% of sequence in fragments shorter than    1044 bp
  40% of sequence in fragments shorter than    1175 bp
  45% of sequence in fragments shorter than    1306 bp
  50% of sequence in fragments shorter than    1447 bp
  55% of sequence in fragments shorter than    1597 bp
  60% of sequence in fragments shorter than    1760 bp
  65% of sequence in fragments shorter than    1946 bp
  70% of sequence in fragments shorter than    2157 bp
  75% of sequence in fragments shorter than    2401 bp
  80% of sequence in fragments shorter than    2701 bp
  85% of sequence in fragments shorter than    3084 bp
  90% of sequence in fragments shorter than    3630 bp
  95% of sequence in fragments shorter than    4649 bp

# SacI.StyI (GAGCTC+CCWWGG) double digest, 3716 thousand fragments (603323+3114107 sites), 801 bp average fragment length
   5% of sequence in fragments shorter than     271 bp
  10% of sequence in fragments shorter than     416 bp
  15% of sequence in fragments shorter than     544 bp
  20% of sequence in fragments shorter than     667 bp
  25% of sequence in fragments shorter than     780 bp
  30% of sequence in fragments shorter than     906 bp
  35% of sequence in fragments shorter than    1035 bp
  40% of sequence in fragments shorter than    1170 bp
  45% of sequence in fragments shorter than    1312 bp
  50% of sequence in fragments shorter than    1461 bp
  55% of sequence in fragments shorter than    1627 bp
  60% of sequence in fragments shorter than    1810 bp
  65% of sequence in fragments shorter than    2015 bp
  70% of sequence in fragments shorter than    2249 bp
  75% of sequence in fragments shorter than    2501 bp
  80% of sequence in fragments shorter than    2834 bp
  85% of sequence in fragments shorter than    3275 bp
  90% of sequence in fragments shorter than    3911 bp
  95% of sequence in fragments shorter than    5142 bp

# BscAI.PspXI (GCATC+VCTCGAGB) double digest, 3711 thousand fragments (3652926+59193 sites), 802 bp average fragment length
   5% of sequence in fragments shorter than     273 bp
  10% of sequence in fragments shorter than     424 bp
  15% of sequence in fragments shorter than     557 bp
  20% of sequence in fragments shorter than     678 bp
  25% of sequence in fragments shorter than     795 bp
  30% of sequence in fragments shorter than     917 bp
  35% of sequence in fragments shorter than    1042 bp
  40% of sequence in fragments shorter than    1172 bp
  45% of sequence in fragments shorter than    1303 bp
  50% of sequence in fragments shorter than    1442 bp
  55% of sequence in fragments shorter than    1593 bp
  60% of sequence in fragments shorter than    1754 bp
  65% of sequence in fragments shorter than    1939 bp
  70% of sequence in fragments shorter than    2148 bp
  75% of sequence in fragments shorter than    2391 bp
  80% of sequence in fragments shorter than    2689 bp
  85% of sequence in fragments shorter than    3068 bp
  90% of sequence in fragments shorter than    3612 bp
  95% of sequence in fragments shorter than    4623 bp

# NspI.SacI (RCATGY+GAGCTC) double digest, 3707 thousand fragments (3104359+603323 sites), 803 bp average fragment length
   5% of sequence in fragments shorter than     280 bp
  10% of sequence in fragments shorter than     427 bp
  15% of sequence in fragments shorter than     559 bp
  20% of sequence in fragments shorter than     682 bp
  25% of sequence in fragments shorter than     799 bp
  30% of sequence in fragments shorter than     917 bp
  35% of sequence in fragments shorter than    1039 bp
  40% of sequence in fragments shorter than    1162 bp
  45% of sequence in fragments shorter than    1293 bp
  50% of sequence in fragments shorter than    1431 bp
  55% of sequence in fragments shorter than    1580 bp
  60% of sequence in fragments shorter than    1742 bp
  65% of sequence in fragments shorter than    1921 bp
  70% of sequence in fragments shorter than    2120 bp
  75% of sequence in fragments shorter than    2358 bp
  80% of sequence in fragments shorter than    2644 bp
  85% of sequence in fragments shorter than    3008 bp
  90% of sequence in fragments shorter than    3511 bp
  95% of sequence in fragments shorter than    4379 bp

# AgeI.BscAI (ACCGGT+GCATC) double digest, 3705 thousand fragments (53386+3652926 sites), 804 bp average fragment length
   5% of sequence in fragments shorter than     273 bp
  10% of sequence in fragments shorter than     425 bp
  15% of sequence in fragments shorter than     558 bp
  20% of sequence in fragments shorter than     678 bp
  25% of sequence in fragments shorter than     797 bp
  30% of sequence in fragments shorter than     919 bp
  35% of sequence in fragments shorter than    1044 bp
  40% of sequence in fragments shorter than    1174 bp
  45% of sequence in fragments shorter than    1305 bp
  50% of sequence in fragments shorter than    1445 bp
  55% of sequence in fragments shorter than    1595 bp
  60% of sequence in fragments shorter than    1757 bp
  65% of sequence in fragments shorter than    1942 bp
  70% of sequence in fragments shorter than    2151 bp
  75% of sequence in fragments shorter than    2394 bp
  80% of sequence in fragments shorter than    2692 bp
  85% of sequence in fragments shorter than    3073 bp
  90% of sequence in fragments shorter than    3619 bp
  95% of sequence in fragments shorter than    4632 bp

# BspHI.SmlI (TCATGA+CTYRAG) double digest, 3705 thousand fragments (978290+2727867 sites), 804 bp average fragment length
   5% of sequence in fragments shorter than     277 bp
  10% of sequence in fragments shorter than     423 bp
  15% of sequence in fragments shorter than     544 bp
  20% of sequence in fragments shorter than     660 bp
  25% of sequence in fragments shorter than     773 bp
  30% of sequence in fragments shorter than     887 bp
  35% of sequence in fragments shorter than    1002 bp
  40% of sequence in fragments shorter than    1121 bp
  45% of sequence in fragments shorter than    1245 bp
  50% of sequence in fragments shorter than    1379 bp
  55% of sequence in fragments shorter than    1521 bp
  60% of sequence in fragments shorter than    1680 bp
  65% of sequence in fragments shorter than    1853 bp
  70% of sequence in fragments shorter than    2049 bp
  75% of sequence in fragments shorter than    2264 bp
  80% of sequence in fragments shorter than    2539 bp
  85% of sequence in fragments shorter than    2853 bp
  90% of sequence in fragments shorter than    3342 bp
  95% of sequence in fragments shorter than    4231 bp

# AclI.SfeI (AACGTT+CTRYAG) double digest, 3703 thousand fragments (172706+3531009 sites), 804 bp average fragment length
   5% of sequence in fragments shorter than     282 bp
  10% of sequence in fragments shorter than     424 bp
  15% of sequence in fragments shorter than     544 bp
  20% of sequence in fragments shorter than     660 bp
  25% of sequence in fragments shorter than     775 bp
  30% of sequence in fragments shorter than     891 bp
  35% of sequence in fragments shorter than    1009 bp
  40% of sequence in fragments shorter than    1122 bp
  45% of sequence in fragments shorter than    1249 bp
  50% of sequence in fragments shorter than    1380 bp
  55% of sequence in fragments shorter than    1519 bp
  60% of sequence in fragments shorter than    1676 bp
  65% of sequence in fragments shorter than    1845 bp
  70% of sequence in fragments shorter than    2037 bp
  75% of sequence in fragments shorter than    2237 bp
  80% of sequence in fragments shorter than    2503 bp
  85% of sequence in fragments shorter than    2839 bp
  90% of sequence in fragments shorter than    3309 bp
  95% of sequence in fragments shorter than    4135 bp

# AvrII.NspI (CCTAGG+RCATGY) double digest, 3698 thousand fragments (594956+3104359 sites), 805 bp average fragment length
   5% of sequence in fragments shorter than     282 bp
  10% of sequence in fragments shorter than     432 bp
  15% of sequence in fragments shorter than     566 bp
  20% of sequence in fragments shorter than     676 bp
  25% of sequence in fragments shorter than     795 bp
  30% of sequence in fragments shorter than     916 bp
  35% of sequence in fragments shorter than    1039 bp
  40% of sequence in fragments shorter than    1163 bp
  45% of sequence in fragments shorter than    1293 bp
  50% of sequence in fragments shorter than    1432 bp
  55% of sequence in fragments shorter than    1582 bp
  60% of sequence in fragments shorter than    1743 bp
  65% of sequence in fragments shorter than    1923 bp
  70% of sequence in fragments shorter than    2123 bp
  75% of sequence in fragments shorter than    2356 bp
  80% of sequence in fragments shorter than    2638 bp
  85% of sequence in fragments shorter than    3005 bp
  90% of sequence in fragments shorter than    3526 bp
  95% of sequence in fragments shorter than    4518 bp

# PstI.SspI (CTGCAG+AATATT) double digest, 3698 thousand fragments (1321469+2377267 sites), 805 bp average fragment length
   5% of sequence in fragments shorter than     277 bp
  10% of sequence in fragments shorter than     422 bp
  15% of sequence in fragments shorter than     551 bp
  20% of sequence in fragments shorter than     669 bp
  25% of sequence in fragments shorter than     786 bp
  30% of sequence in fragments shorter than     906 bp
  35% of sequence in fragments shorter than    1026 bp
  40% of sequence in fragments shorter than    1152 bp
  45% of sequence in fragments shorter than    1284 bp
  50% of sequence in fragments shorter than    1419 bp
  55% of sequence in fragments shorter than    1569 bp
  60% of sequence in fragments shorter than    1734 bp
  65% of sequence in fragments shorter than    1917 bp
  70% of sequence in fragments shorter than    2127 bp
  75% of sequence in fragments shorter than    2374 bp
  80% of sequence in fragments shorter than    2675 bp
  85% of sequence in fragments shorter than    3057 bp
  90% of sequence in fragments shorter than    3617 bp
  95% of sequence in fragments shorter than    4579 bp

# BscAI.PmeI (GCATC+GTTTAAAC) double digest, 3694 thousand fragments (3652926+42094 sites), 806 bp average fragment length
   5% of sequence in fragments shorter than     275 bp
  10% of sequence in fragments shorter than     427 bp
  15% of sequence in fragments shorter than     560 bp
  20% of sequence in fragments shorter than     680 bp
  25% of sequence in fragments shorter than     798 bp
  30% of sequence in fragments shorter than     920 bp
  35% of sequence in fragments shorter than    1046 bp
  40% of sequence in fragments shorter than    1176 bp
  45% of sequence in fragments shorter than    1307 bp
  50% of sequence in fragments shorter than    1446 bp
  55% of sequence in fragments shorter than    1596 bp
  60% of sequence in fragments shorter than    1757 bp
  65% of sequence in fragments shorter than    1942 bp
  70% of sequence in fragments shorter than    2151 bp
  75% of sequence in fragments shorter than    2393 bp
  80% of sequence in fragments shorter than    2690 bp
  85% of sequence in fragments shorter than    3070 bp
  90% of sequence in fragments shorter than    3613 bp
  95% of sequence in fragments shorter than    4618 bp

# PacI.SfeI (TTAATTAA+CTRYAG) double digest, 3690 thousand fragments (159455+3531009 sites), 807 bp average fragment length
   5% of sequence in fragments shorter than     284 bp
  10% of sequence in fragments shorter than     427 bp
  15% of sequence in fragments shorter than     548 bp
  20% of sequence in fragments shorter than     665 bp
  25% of sequence in fragments shorter than     780 bp
  30% of sequence in fragments shorter than     897 bp
  35% of sequence in fragments shorter than    1015 bp
  40% of sequence in fragments shorter than    1130 bp
  45% of sequence in fragments shorter than    1257 bp
  50% of sequence in fragments shorter than    1386 bp
  55% of sequence in fragments shorter than    1529 bp
  60% of sequence in fragments shorter than    1684 bp
  65% of sequence in fragments shorter than    1853 bp
  70% of sequence in fragments shorter than    2045 bp
  75% of sequence in fragments shorter than    2245 bp
  80% of sequence in fragments shorter than    2513 bp
  85% of sequence in fragments shorter than    2851 bp
  90% of sequence in fragments shorter than    3324 bp
  95% of sequence in fragments shorter than    4155 bp

# Hpy99I.SfeI (CGWCG+CTRYAG) double digest, 3688 thousand fragments (157917+3531009 sites), 807 bp average fragment length
   5% of sequence in fragments shorter than     283 bp
  10% of sequence in fragments shorter than     426 bp
  15% of sequence in fragments shorter than     548 bp
  20% of sequence in fragments shorter than     667 bp
  25% of sequence in fragments shorter than     784 bp
  30% of sequence in fragments shorter than     902 bp
  35% of sequence in fragments shorter than    1022 bp
  40% of sequence in fragments shorter than    1140 bp
  45% of sequence in fragments shorter than    1269 bp
  50% of sequence in fragments shorter than    1401 bp
  55% of sequence in fragments shorter than    1549 bp
  60% of sequence in fragments shorter than    1708 bp
  65% of sequence in fragments shorter than    1883 bp
  70% of sequence in fragments shorter than    2080 bp
  75% of sequence in fragments shorter than    2287 bp
  80% of sequence in fragments shorter than    2566 bp
  85% of sequence in fragments shorter than    2911 bp
  90% of sequence in fragments shorter than    3405 bp
  95% of sequence in fragments shorter than    4263 bp

# HgaI.StyI (GACGC+CCWWGG) double digest, 3685 thousand fragments (571889+3114107 sites), 808 bp average fragment length
   5% of sequence in fragments shorter than     269 bp
  10% of sequence in fragments shorter than     417 bp
  15% of sequence in fragments shorter than     548 bp
  20% of sequence in fragments shorter than     675 bp
  25% of sequence in fragments shorter than     795 bp
  30% of sequence in fragments shorter than     926 bp
  35% of sequence in fragments shorter than    1058 bp
  40% of sequence in fragments shorter than    1198 bp
  45% of sequence in fragments shorter than    1346 bp
  50% of sequence in fragments shorter than    1503 bp
  55% of sequence in fragments shorter than    1678 bp
  60% of sequence in fragments shorter than    1868 bp
  65% of sequence in fragments shorter than    2082 bp
  70% of sequence in fragments shorter than    2331 bp
  75% of sequence in fragments shorter than    2621 bp
  80% of sequence in fragments shorter than    2971 bp
  85% of sequence in fragments shorter than    3415 bp
  90% of sequence in fragments shorter than    4083 bp
  95% of sequence in fragments shorter than    5361 bp

# EciI.StyI (GGCGGA+CCWWGG) double digest, 3684 thousand fragments (571273+3114107 sites), 808 bp average fragment length
   5% of sequence in fragments shorter than     267 bp
  10% of sequence in fragments shorter than     415 bp
  15% of sequence in fragments shorter than     543 bp
  20% of sequence in fragments shorter than     668 bp
  25% of sequence in fragments shorter than     784 bp
  30% of sequence in fragments shorter than     912 bp
  35% of sequence in fragments shorter than    1044 bp
  40% of sequence in fragments shorter than    1183 bp
  45% of sequence in fragments shorter than    1328 bp
  50% of sequence in fragments shorter than    1480 bp
  55% of sequence in fragments shorter than    1653 bp
  60% of sequence in fragments shorter than    1843 bp
  65% of sequence in fragments shorter than    2061 bp
  70% of sequence in fragments shorter than    2310 bp
  75% of sequence in fragments shorter than    2603 bp
  80% of sequence in fragments shorter than    2955 bp
  85% of sequence in fragments shorter than    3384 bp
  90% of sequence in fragments shorter than    4068 bp
  95% of sequence in fragments shorter than    5403 bp

# BscAI.SalI (GCATC+GTCGAC) double digest, 3683 thousand fragments (3652926+31396 sites), 808 bp average fragment length
   5% of sequence in fragments shorter than     275 bp
  10% of sequence in fragments shorter than     428 bp
  15% of sequence in fragments shorter than     562 bp
  20% of sequence in fragments shorter than     683 bp
  25% of sequence in fragments shorter than     801 bp
  30% of sequence in fragments shorter than     924 bp
  35% of sequence in fragments shorter than    1050 bp
  40% of sequence in fragments shorter than    1181 bp
  45% of sequence in fragments shorter than    1313 bp
  50% of sequence in fragments shorter than    1453 bp
  55% of sequence in fragments shorter than    1601 bp
  60% of sequence in fragments shorter than    1767 bp
  65% of sequence in fragments shorter than    1953 bp
  70% of sequence in fragments shorter than    2163 bp
  75% of sequence in fragments shorter than    2408 bp
  80% of sequence in fragments shorter than    2708 bp
  85% of sequence in fragments shorter than    3091 bp
  90% of sequence in fragments shorter than    3638 bp
  95% of sequence in fragments shorter than    4653 bp

# AvaII.AvaIII (GGWCC+ATGCAT) double digest, 3680 thousand fragments (2747690+933357 sites), 809 bp average fragment length
   5% of sequence in fragments shorter than     272 bp
  10% of sequence in fragments shorter than     417 bp
  15% of sequence in fragments shorter than     554 bp
  20% of sequence in fragments shorter than     687 bp
  25% of sequence in fragments shorter than     818 bp
  30% of sequence in fragments shorter than     939 bp
  35% of sequence in fragments shorter than    1075 bp
  40% of sequence in fragments shorter than    1217 bp
  45% of sequence in fragments shorter than    1367 bp
  50% of sequence in fragments shorter than    1525 bp
  55% of sequence in fragments shorter than    1698 bp
  60% of sequence in fragments shorter than    1880 bp
  65% of sequence in fragments shorter than    2086 bp
  70% of sequence in fragments shorter than    2320 bp
  75% of sequence in fragments shorter than    2595 bp
  80% of sequence in fragments shorter than    2920 bp
  85% of sequence in fragments shorter than    3344 bp
  90% of sequence in fragments shorter than    3950 bp
  95% of sequence in fragments shorter than    5050 bp

# MfeI.StyI (CAATTG+CCWWGG) double digest, 3677 thousand fragments (564303+3114107 sites), 810 bp average fragment length
   5% of sequence in fragments shorter than     279 bp
  10% of sequence in fragments shorter than     426 bp
  15% of sequence in fragments shorter than     551 bp
  20% of sequence in fragments shorter than     672 bp
  25% of sequence in fragments shorter than     781 bp
  30% of sequence in fragments shorter than     902 bp
  35% of sequence in fragments shorter than    1025 bp
  40% of sequence in fragments shorter than    1152 bp
  45% of sequence in fragments shorter than    1286 bp
  50% of sequence in fragments shorter than    1424 bp
  55% of sequence in fragments shorter than    1579 bp
  60% of sequence in fragments shorter than    1749 bp
  65% of sequence in fragments shorter than    1934 bp
  70% of sequence in fragments shorter than    2146 bp
  75% of sequence in fragments shorter than    2397 bp
  80% of sequence in fragments shorter than    2703 bp
  85% of sequence in fragments shorter than    3086 bp
  90% of sequence in fragments shorter than    3595 bp
  95% of sequence in fragments shorter than    4584 bp

# HgaI.NspI (GACGC+RCATGY) double digest, 3675 thousand fragments (571889+3104359 sites), 810 bp average fragment length
   5% of sequence in fragments shorter than     282 bp
  10% of sequence in fragments shorter than     432 bp
  15% of sequence in fragments shorter than     567 bp
  20% of sequence in fragments shorter than     692 bp
  25% of sequence in fragments shorter than     814 bp
  30% of sequence in fragments shorter than     936 bp
  35% of sequence in fragments shorter than    1060 bp
  40% of sequence in fragments shorter than    1186 bp
  45% of sequence in fragments shorter than    1319 bp
  50% of sequence in fragments shorter than    1461 bp
  55% of sequence in fragments shorter than    1613 bp
  60% of sequence in fragments shorter than    1776 bp
  65% of sequence in fragments shorter than    1957 bp
  70% of sequence in fragments shorter than    2158 bp
  75% of sequence in fragments shorter than    2400 bp
  80% of sequence in fragments shorter than    2683 bp
  85% of sequence in fragments shorter than    3037 bp
  90% of sequence in fragments shorter than    3546 bp
  95% of sequence in fragments shorter than    4476 bp

# EciI.NspI (GGCGGA+RCATGY) double digest, 3675 thousand fragments (571273+3104359 sites), 810 bp average fragment length
   5% of sequence in fragments shorter than     278 bp
  10% of sequence in fragments shorter than     429 bp
  15% of sequence in fragments shorter than     563 bp
  20% of sequence in fragments shorter than     687 bp
  25% of sequence in fragments shorter than     808 bp
  30% of sequence in fragments shorter than     929 bp
  35% of sequence in fragments shorter than    1053 bp
  40% of sequence in fragments shorter than    1179 bp
  45% of sequence in fragments shorter than    1311 bp
  50% of sequence in fragments shorter than    1453 bp
  55% of sequence in fragments shorter than    1606 bp
  60% of sequence in fragments shorter than    1770 bp
  65% of sequence in fragments shorter than    1954 bp
  70% of sequence in fragments shorter than    2159 bp
  75% of sequence in fragments shorter than    2404 bp
  80% of sequence in fragments shorter than    2706 bp
  85% of sequence in fragments shorter than    3083 bp
  90% of sequence in fragments shorter than    3619 bp
  95% of sequence in fragments shorter than    4645 bp

# BscAI.MluI (GCATC+ACGCGT) double digest, 3673 thousand fragments (3652926+21415 sites), 811 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     429 bp
  15% of sequence in fragments shorter than     563 bp
  20% of sequence in fragments shorter than     685 bp
  25% of sequence in fragments shorter than     803 bp
  30% of sequence in fragments shorter than     926 bp
  35% of sequence in fragments shorter than    1053 bp
  40% of sequence in fragments shorter than    1183 bp
  45% of sequence in fragments shorter than    1316 bp
  50% of sequence in fragments shorter than    1457 bp
  55% of sequence in fragments shorter than    1605 bp
  60% of sequence in fragments shorter than    1772 bp
  65% of sequence in fragments shorter than    1958 bp
  70% of sequence in fragments shorter than    2169 bp
  75% of sequence in fragments shorter than    2414 bp
  80% of sequence in fragments shorter than    2715 bp
  85% of sequence in fragments shorter than    3099 bp
  90% of sequence in fragments shorter than    3646 bp
  95% of sequence in fragments shorter than    4667 bp

# AvaII.StuI (GGWCC+AGGCCT) double digest, 3672 thousand fragments (2747690+925729 sites), 811 bp average fragment length
   5% of sequence in fragments shorter than     256 bp
  10% of sequence in fragments shorter than     398 bp
  15% of sequence in fragments shorter than     544 bp
  20% of sequence in fragments shorter than     690 bp
  25% of sequence in fragments shorter than     842 bp
  30% of sequence in fragments shorter than     989 bp
  35% of sequence in fragments shorter than    1144 bp
  40% of sequence in fragments shorter than    1314 bp
  45% of sequence in fragments shorter than    1490 bp
  50% of sequence in fragments shorter than    1680 bp
  55% of sequence in fragments shorter than    1889 bp
  60% of sequence in fragments shorter than    2118 bp
  65% of sequence in fragments shorter than    2375 bp
  70% of sequence in fragments shorter than    2667 bp
  75% of sequence in fragments shorter than    3007 bp
  80% of sequence in fragments shorter than    3419 bp
  85% of sequence in fragments shorter than    3953 bp
  90% of sequence in fragments shorter than    4699 bp
  95% of sequence in fragments shorter than    5973 bp

# MfeI.NspI (CAATTG+RCATGY) double digest, 3668 thousand fragments (564303+3104359 sites), 812 bp average fragment length
   5% of sequence in fragments shorter than     283 bp
  10% of sequence in fragments shorter than     434 bp
  15% of sequence in fragments shorter than     566 bp
  20% of sequence in fragments shorter than     689 bp
  25% of sequence in fragments shorter than     808 bp
  30% of sequence in fragments shorter than     928 bp
  35% of sequence in fragments shorter than    1050 bp
  40% of sequence in fragments shorter than    1176 bp
  45% of sequence in fragments shorter than    1307 bp
  50% of sequence in fragments shorter than    1438 bp
  55% of sequence in fragments shorter than    1588 bp
  60% of sequence in fragments shorter than    1750 bp
  65% of sequence in fragments shorter than    1930 bp
  70% of sequence in fragments shorter than    2130 bp
  75% of sequence in fragments shorter than    2364 bp
  80% of sequence in fragments shorter than    2649 bp
  85% of sequence in fragments shorter than    3021 bp
  90% of sequence in fragments shorter than    3546 bp
  95% of sequence in fragments shorter than    4513 bp

# BscAI.SgrAI (GCATC+CRCCGGYG) double digest, 3667 thousand fragments (3652926+15270 sites), 812 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     564 bp
  20% of sequence in fragments shorter than     686 bp
  25% of sequence in fragments shorter than     805 bp
  30% of sequence in fragments shorter than     928 bp
  35% of sequence in fragments shorter than    1055 bp
  40% of sequence in fragments shorter than    1185 bp
  45% of sequence in fragments shorter than    1319 bp
  50% of sequence in fragments shorter than    1460 bp
  55% of sequence in fragments shorter than    1607 bp
  60% of sequence in fragments shorter than    1775 bp
  65% of sequence in fragments shorter than    1961 bp
  70% of sequence in fragments shorter than    2173 bp
  75% of sequence in fragments shorter than    2418 bp
  80% of sequence in fragments shorter than    2720 bp
  85% of sequence in fragments shorter than    3105 bp
  90% of sequence in fragments shorter than    3653 bp
  95% of sequence in fragments shorter than    4677 bp

# BscAI.NruI (GCATC+TCGCGA) double digest, 3667 thousand fragments (3652926+14872 sites), 812 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     564 bp
  20% of sequence in fragments shorter than     686 bp
  25% of sequence in fragments shorter than     804 bp
  30% of sequence in fragments shorter than     928 bp
  35% of sequence in fragments shorter than    1055 bp
  40% of sequence in fragments shorter than    1185 bp
  45% of sequence in fragments shorter than    1318 bp
  50% of sequence in fragments shorter than    1459 bp
  55% of sequence in fragments shorter than    1607 bp
  60% of sequence in fragments shorter than    1774 bp
  65% of sequence in fragments shorter than    1960 bp
  70% of sequence in fragments shorter than    2171 bp
  75% of sequence in fragments shorter than    2417 bp
  80% of sequence in fragments shorter than    2717 bp
  85% of sequence in fragments shorter than    3101 bp
  90% of sequence in fragments shorter than    3648 bp
  95% of sequence in fragments shorter than    4669 bp

# BscAI.PvuI (GCATC+CGATCG) double digest, 3666 thousand fragments (3652926+14186 sites), 812 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     564 bp
  20% of sequence in fragments shorter than     686 bp
  25% of sequence in fragments shorter than     805 bp
  30% of sequence in fragments shorter than     928 bp
  35% of sequence in fragments shorter than    1056 bp
  40% of sequence in fragments shorter than    1185 bp
  45% of sequence in fragments shorter than    1319 bp
  50% of sequence in fragments shorter than    1460 bp
  55% of sequence in fragments shorter than    1608 bp
  60% of sequence in fragments shorter than    1775 bp
  65% of sequence in fragments shorter than    1961 bp
  70% of sequence in fragments shorter than    2173 bp
  75% of sequence in fragments shorter than    2419 bp
  80% of sequence in fragments shorter than    2720 bp
  85% of sequence in fragments shorter than    3104 bp
  90% of sequence in fragments shorter than    3652 bp
  95% of sequence in fragments shorter than    4671 bp

# BscAI.FseI (GCATC+GGCCGGCC) double digest, 3666 thousand fragments (3652926+13662 sites), 812 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     564 bp
  20% of sequence in fragments shorter than     686 bp
  25% of sequence in fragments shorter than     805 bp
  30% of sequence in fragments shorter than     928 bp
  35% of sequence in fragments shorter than    1055 bp
  40% of sequence in fragments shorter than    1185 bp
  45% of sequence in fragments shorter than    1319 bp
  50% of sequence in fragments shorter than    1460 bp
  55% of sequence in fragments shorter than    1608 bp
  60% of sequence in fragments shorter than    1775 bp
  65% of sequence in fragments shorter than    1962 bp
  70% of sequence in fragments shorter than    2173 bp
  75% of sequence in fragments shorter than    2419 bp
  80% of sequence in fragments shorter than    2721 bp
  85% of sequence in fragments shorter than    3106 bp
  90% of sequence in fragments shorter than    3656 bp
  95% of sequence in fragments shorter than    4680 bp

# SphI.StyI (GCATGC+CCWWGG) double digest, 3664 thousand fragments (550952+3114107 sites), 813 bp average fragment length
   5% of sequence in fragments shorter than     275 bp
  10% of sequence in fragments shorter than     422 bp
  15% of sequence in fragments shorter than     551 bp
  20% of sequence in fragments shorter than     674 bp
  25% of sequence in fragments shorter than     789 bp
  30% of sequence in fragments shorter than     914 bp
  35% of sequence in fragments shorter than    1043 bp
  40% of sequence in fragments shorter than    1177 bp
  45% of sequence in fragments shorter than    1318 bp
  50% of sequence in fragments shorter than    1465 bp
  55% of sequence in fragments shorter than    1630 bp
  60% of sequence in fragments shorter than    1811 bp
  65% of sequence in fragments shorter than    2016 bp
  70% of sequence in fragments shorter than    2252 bp
  75% of sequence in fragments shorter than    2530 bp
  80% of sequence in fragments shorter than    2864 bp
  85% of sequence in fragments shorter than    3273 bp
  90% of sequence in fragments shorter than    3899 bp
  95% of sequence in fragments shorter than    5130 bp

# SfeI.SnaBI (CTRYAG+TACGTA) double digest, 3662 thousand fragments (3531009+132299 sites), 813 bp average fragment length
   5% of sequence in fragments shorter than     286 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     551 bp
  20% of sequence in fragments shorter than     669 bp
  25% of sequence in fragments shorter than     785 bp
  30% of sequence in fragments shorter than     902 bp
  35% of sequence in fragments shorter than    1021 bp
  40% of sequence in fragments shorter than    1137 bp
  45% of sequence in fragments shorter than    1265 bp
  50% of sequence in fragments shorter than    1395 bp
  55% of sequence in fragments shorter than    1540 bp
  60% of sequence in fragments shorter than    1696 bp
  65% of sequence in fragments shorter than    1868 bp
  70% of sequence in fragments shorter than    2063 bp
  75% of sequence in fragments shorter than    2263 bp
  80% of sequence in fragments shorter than    2535 bp
  85% of sequence in fragments shorter than    2874 bp
  90% of sequence in fragments shorter than    3353 bp
  95% of sequence in fragments shorter than    4192 bp

# BscAI.NotI (GCATC+GCGGCCGC) double digest, 3662 thousand fragments (3652926+9795 sites), 813 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     565 bp
  20% of sequence in fragments shorter than     687 bp
  25% of sequence in fragments shorter than     806 bp
  30% of sequence in fragments shorter than     929 bp
  35% of sequence in fragments shorter than    1057 bp
  40% of sequence in fragments shorter than    1187 bp
  45% of sequence in fragments shorter than    1320 bp
  50% of sequence in fragments shorter than    1462 bp
  55% of sequence in fragments shorter than    1610 bp
  60% of sequence in fragments shorter than    1778 bp
  65% of sequence in fragments shorter than    1964 bp
  70% of sequence in fragments shorter than    2177 bp
  75% of sequence in fragments shorter than    2422 bp
  80% of sequence in fragments shorter than    2724 bp
  85% of sequence in fragments shorter than    3109 bp
  90% of sequence in fragments shorter than    3660 bp
  95% of sequence in fragments shorter than    4685 bp

# BscAI.RsrII (GCATC+CGGWCCG) double digest, 3662 thousand fragments (3652926+9764 sites), 813 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     565 bp
  20% of sequence in fragments shorter than     687 bp
  25% of sequence in fragments shorter than     806 bp
  30% of sequence in fragments shorter than     929 bp
  35% of sequence in fragments shorter than    1057 bp
  40% of sequence in fragments shorter than    1187 bp
  45% of sequence in fragments shorter than    1320 bp
  50% of sequence in fragments shorter than    1462 bp
  55% of sequence in fragments shorter than    1610 bp
  60% of sequence in fragments shorter than    1777 bp
  65% of sequence in fragments shorter than    1964 bp
  70% of sequence in fragments shorter than    2176 bp
  75% of sequence in fragments shorter than    2422 bp
  80% of sequence in fragments shorter than    2724 bp
  85% of sequence in fragments shorter than    3109 bp
  90% of sequence in fragments shorter than    3660 bp
  95% of sequence in fragments shorter than    4685 bp

# AvaIII.SmlI (ATGCAT+CTYRAG) double digest, 3660 thousand fragments (933357+2727867 sites), 814 bp average fragment length
   5% of sequence in fragments shorter than     281 bp
  10% of sequence in fragments shorter than     428 bp
  15% of sequence in fragments shorter than     553 bp
  20% of sequence in fragments shorter than     671 bp
  25% of sequence in fragments shorter than     784 bp
  30% of sequence in fragments shorter than     901 bp
  35% of sequence in fragments shorter than    1016 bp
  40% of sequence in fragments shorter than    1137 bp
  45% of sequence in fragments shorter than    1264 bp
  50% of sequence in fragments shorter than    1395 bp
  55% of sequence in fragments shorter than    1541 bp
  60% of sequence in fragments shorter than    1700 bp
  65% of sequence in fragments shorter than    1873 bp
  70% of sequence in fragments shorter than    2066 bp
  75% of sequence in fragments shorter than    2285 bp
  80% of sequence in fragments shorter than    2561 bp
  85% of sequence in fragments shorter than    2874 bp
  90% of sequence in fragments shorter than    3365 bp
  95% of sequence in fragments shorter than    4239 bp

# ScaI.StyI (AGTACT+CCWWGG) double digest, 3657 thousand fragments (543793+3114107 sites), 814 bp average fragment length
   5% of sequence in fragments shorter than     280 bp
  10% of sequence in fragments shorter than     425 bp
  15% of sequence in fragments shorter than     552 bp
  20% of sequence in fragments shorter than     673 bp
  25% of sequence in fragments shorter than     785 bp
  30% of sequence in fragments shorter than     907 bp
  35% of sequence in fragments shorter than    1030 bp
  40% of sequence in fragments shorter than    1159 bp
  45% of sequence in fragments shorter than    1293 bp
  50% of sequence in fragments shorter than    1431 bp
  55% of sequence in fragments shorter than    1585 bp
  60% of sequence in fragments shorter than    1755 bp
  65% of sequence in fragments shorter than    1945 bp
  70% of sequence in fragments shorter than    2163 bp
  75% of sequence in fragments shorter than    2417 bp
  80% of sequence in fragments shorter than    2727 bp
  85% of sequence in fragments shorter than    3121 bp
  90% of sequence in fragments shorter than    3655 bp
  95% of sequence in fragments shorter than    4753 bp

# AscI.BscAI (GGCGCGCC+GCATC) double digest, 3657 thousand fragments (4751+3652926 sites), 814 bp average fragment length
   5% of sequence in fragments shorter than     277 bp
  10% of sequence in fragments shorter than     431 bp
  15% of sequence in fragments shorter than     566 bp
  20% of sequence in fragments shorter than     687 bp
  25% of sequence in fragments shorter than     807 bp
  30% of sequence in fragments shorter than     930 bp
  35% of sequence in fragments shorter than    1058 bp
  40% of sequence in fragments shorter than    1188 bp
  45% of sequence in fragments shorter than    1322 bp
  50% of sequence in fragments shorter than    1464 bp
  55% of sequence in fragments shorter than    1612 bp
  60% of sequence in fragments shorter than    1779 bp
  65% of sequence in fragments shorter than    1965 bp
  70% of sequence in fragments shorter than    2179 bp
  75% of sequence in fragments shorter than    2424 bp
  80% of sequence in fragments shorter than    2726 bp
  85% of sequence in fragments shorter than    3111 bp
  90% of sequence in fragments shorter than    3662 bp
  95% of sequence in fragments shorter than    4687 bp

# NaeI.SfeI (GCCGGC+CTRYAG) double digest, 3656 thousand fragments (125692+3531009 sites), 815 bp average fragment length
   5% of sequence in fragments shorter than     285 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     553 bp
  20% of sequence in fragments shorter than     672 bp
  25% of sequence in fragments shorter than     790 bp
  30% of sequence in fragments shorter than     909 bp
  35% of sequence in fragments shorter than    1030 bp
  40% of sequence in fragments shorter than    1150 bp
  45% of sequence in fragments shorter than    1280 bp
  50% of sequence in fragments shorter than    1413 bp
  55% of sequence in fragments shorter than    1562 bp
  60% of sequence in fragments shorter than    1723 bp
  65% of sequence in fragments shorter than    1899 bp
  70% of sequence in fragments shorter than    2097 bp
  75% of sequence in fragments shorter than    2307 bp
  80% of sequence in fragments shorter than    2587 bp
  85% of sequence in fragments shorter than    2939 bp
  90% of sequence in fragments shorter than    3441 bp
  95% of sequence in fragments shorter than    4313 bp

# SmlI.StuI (CTYRAG+AGGCCT) double digest, 3653 thousand fragments (2727867+925729 sites), 815 bp average fragment length
   5% of sequence in fragments shorter than     284 bp
  10% of sequence in fragments shorter than     418 bp
  15% of sequence in fragments shorter than     549 bp
  20% of sequence in fragments shorter than     674 bp
  25% of sequence in fragments shorter than     784 bp
  30% of sequence in fragments shorter than     905 bp
  35% of sequence in fragments shorter than    1028 bp
  40% of sequence in fragments shorter than    1155 bp
  45% of sequence in fragments shorter than    1289 bp
  50% of sequence in fragments shorter than    1426 bp
  55% of sequence in fragments shorter than    1580 bp
  60% of sequence in fragments shorter than    1748 bp
  65% of sequence in fragments shorter than    1933 bp
  70% of sequence in fragments shorter than    2127 bp
  75% of sequence in fragments shorter than    2356 bp
  80% of sequence in fragments shorter than    2642 bp
  85% of sequence in fragments shorter than    3003 bp
  90% of sequence in fragments shorter than    3505 bp
  95% of sequence in fragments shorter than    4360 bp

# SfeI.XhoI (CTRYAG+CTCGAG) double digest, 3651 thousand fragments (3531009+121308 sites), 816 bp average fragment length
   5% of sequence in fragments shorter than     285 bp
  10% of sequence in fragments shorter than     430 bp
  15% of sequence in fragments shorter than     553 bp
  20% of sequence in fragments shorter than     673 bp
  25% of sequence in fragments shorter than     790 bp
  30% of sequence in fragments shorter than     908 bp
  35% of sequence in fragments shorter than    1026 bp
  40% of sequence in fragments shorter than    1147 bp
  45% of sequence in fragments shorter than    1277 bp
  50% of sequence in fragments shorter than    1409 bp
  55% of sequence in fragments shorter than    1557 bp
  60% of sequence in fragments shorter than    1717 bp
  65% of sequence in fragments shorter than    1892 bp
  70% of sequence in fragments shorter than    2089 bp
  75% of sequence in fragments shorter than    2297 bp
  80% of sequence in fragments shorter than    2576 bp
  85% of sequence in fragments shorter than    2924 bp
  90% of sequence in fragments shorter than    3419 bp
  95% of sequence in fragments shorter than    4288 bp

# AvaII.NdeI (GGWCC+CATATG) double digest, 3650 thousand fragments (2747690+903360 sites), 816 bp average fragment length
   5% of sequence in fragments shorter than     276 bp
  10% of sequence in fragments shorter than     418 bp
  15% of sequence in fragments shorter than     559 bp
  20% of sequence in fragments shorter than     690 bp
  25% of sequence in fragments shorter than     821 bp
  30% of sequence in fragments shorter than     944 bp
  35% of sequence in fragments shorter than    1079 bp
  40% of sequence in fragments shorter than    1211 bp
  45% of sequence in fragments shorter than    1360 bp
  50% of sequence in fragments shorter than    1515 bp
  55% of sequence in fragments shorter than    1687 bp
  60% of sequence in fragments shorter than    1867 bp
  65% of sequence in fragments shorter than    2075 bp
  70% of sequence in fragments shorter than    2310 bp
  75% of sequence in fragments shorter than    2587 bp
  80% of sequence in fragments shorter than    2911 bp
  85% of sequence in fragments shorter than    3339 bp
  90% of sequence in fragments shorter than    3951 bp
  95% of sequence in fragments shorter than    5078 bp

# NspI.ScaI (RCATGY+AGTACT) double digest, 3647 thousand fragments (3104359+543793 sites), 816 bp average fragment length
   5% of sequence in fragments shorter than     283 bp
  10% of sequence in fragments shorter than     434 bp
  15% of sequence in fragments shorter than     568 bp
  20% of sequence in fragments shorter than     692 bp
  25% of sequence in fragments shorter than     813 bp
  30% of sequence in fragments shorter than     934 bp
  35% of sequence in fragments shorter than    1058 bp
  40% of sequence in fragments shorter than    1184 bp
  45% of sequence in fragments shorter than    1318 bp
  50% of sequence in fragments shorter than    1459 bp
  55% of sequence in fragments shorter than    1611 bp
  60% of sequence in fragments shorter than    1776 bp
  65% of sequence in fragments shorter than    1959 bp
  70% of sequence in fragments shorter than    2164 bp
  75% of sequence in fragments shorter than    2409 bp
  80% of sequence in fragments shorter than    2707 bp
  85% of sequence in fragments shorter than    3085 bp
  90% of sequence in fragments shorter than    3631 bp
  95% of sequence in fragments shorter than    4694 bp

# HpaII.PstI (CCGG+CTGCAG) double digest, 3638 thousand fragments (2317722+1321469 sites), 818 bp average fragment length
   5% of sequence in fragments shorter than     245 bp
  10% of sequence in fragments shorter than     432 bp
  15% of sequence in fragments shorter than     603 bp
  20% of sequence in fragments shorter than     769 bp
  25% of sequence in fragments shorter than     945 bp
  30% of sequence in fragments shorter than    1127 bp
  35% of sequence in fragments shorter than    1317 bp
  40% of sequence in fragments shorter than    1525 bp
  45% of sequence in fragments shorter than    1748 bp
  50% of sequence in fragments shorter than    1993 bp
  55% of sequence in fragments shorter than    2254 bp
  60% of sequence in fragments shorter than    2558 bp
  65% of sequence in fragments shorter than    2900 bp
  70% of sequence in fragments shorter than    3298 bp
  75% of sequence in fragments shorter than    3776 bp
  80% of sequence in fragments shorter than    4353 bp
  85% of sequence in fragments shorter than    5095 bp
  90% of sequence in fragments shorter than    6170 bp
  95% of sequence in fragments shorter than    8192 bp

# NdeI.SmlI (CATATG+CTYRAG) double digest, 3630 thousand fragments (903360+2727867 sites), 820 bp average fragment length
   5% of sequence in fragments shorter than     284 bp
  10% of sequence in fragments shorter than     424 bp
  15% of sequence in fragments shorter than     552 bp
  20% of sequence in fragments shorter than     671 bp
  25% of sequence in fragments shorter than     785 bp
  30% of sequence in fragments shorter than     902 bp
  35% of sequence in fragments shorter than    1017 bp
  40% of sequence in fragments shorter than    1139 bp
  45% of sequence in fragments shorter than    1261 bp
  50% of sequence in fragments shorter than    1396 bp
  55% of sequence in fragments shorter than    1542 bp
  60% of sequence in fragments shorter than    1701 bp
  65% of sequence in fragments shorter than    1876 bp
  70% of sequence in fragments shorter than    2072 bp
  75% of sequence in fragments shorter than    2298 bp
  80% of sequence in fragments shorter than    2580 bp
  85% of sequence in fragments shorter than    2915 bp
  90% of sequence in fragments shorter than    3426 bp
  95% of sequence in fragments shorter than    4367 bp

# ClaI.SfeI (ATCGAT+CTRYAG) double digest, 3617 thousand fragments (86477+3531009 sites), 823 bp average fragment length
   5% of sequence in fragments shorter than     290 bp
  10% of sequence in fragments shorter than     436 bp
  15% of sequence in fragments shorter than     558 bp
  20% of sequence in fragments shorter than     677 bp
  25% of sequence in fragments shorter than     795 bp
  30% of sequence in fragments shorter than     914 bp
  35% of sequence in fragments shorter than    1033 bp
  40% of sequence in fragments shorter than    1150 bp
  45% of sequence in fragments shorter than    1279 bp
  50% of sequence in fragments shorter than    1411 bp
  55% of sequence in fragments shorter than    1558 bp
  60% of sequence in fragments shorter than    1716 bp
  65% of sequence in fragments shorter than    1891 bp
  70% of sequence in fragments shorter than    2086 bp
  75% of sequence in fragments shorter than    2298 bp
  80% of sequence in fragments shorter than    2576 bp
  85% of sequence in fragments shorter than    2922 bp
  90% of sequence in fragments shorter than    3411 bp
  95% of sequence in fragments shorter than    4273 bp

# AvaI.SmlI (CYCGRG+CTYRAG) double digest, 3611 thousand fragments (1005714+2727867 sites), 825 bp average fragment length
   5% of sequence in fragments shorter than     277 bp
  10% of sequence in fragments shorter than     428 bp
  15% of sequence in fragments shorter than     560 bp
  20% of sequence in fragments shorter than     685 bp
  25% of sequence in fragments shorter than     808 bp
  30% of sequence in fragments shorter than     934 bp
  35% of sequence in fragments shorter than    1059 bp
  40% of sequence in fragments shorter than    1193 bp
  45% of sequence in fragments shorter than    1335 bp
  50% of sequence in fragments shorter than    1483 bp
  55% of sequence in fragments shorter than    1650 bp
  60% of sequence in fragments shorter than    1831 bp
  65% of sequence in fragments shorter than    2031 bp
  70% of sequence in fragments shorter than    2254 bp
  75% of sequence in fragments shorter than    2524 bp
  80% of sequence in fragments shorter than    2819 bp
  85% of sequence in fragments shorter than    3238 bp
  90% of sequence in fragments shorter than    3840 bp
  95% of sequence in fragments shorter than    4988 bp

# ApaLI.StyI (GTGCAC+CCWWGG) double digest, 3609 thousand fragments (496255+3114107 sites), 825 bp average fragment length
   5% of sequence in fragments shorter than     278 bp
  10% of sequence in fragments shorter than     424 bp
  15% of sequence in fragments shorter than     556 bp
  20% of sequence in fragments shorter than     684 bp
  25% of sequence in fragments shorter than     802 bp
  30% of sequence in fragments shorter than     932 bp
  35% of sequence in fragments shorter than    1063 bp
  40% of sequence in fragments shorter than    1200 bp
  45% of sequence in fragments shorter than    1345 bp
  50% of sequence in fragments shorter than    1497 bp
  55% of sequence in fragments shorter than    1667 bp
  60% of sequence in fragments shorter than    1853 bp
  65% of sequence in fragments shorter than    2066 bp
  70% of sequence in fragments shorter than    2308 bp
  75% of sequence in fragments shorter than    2592 bp
  80% of sequence in fragments shorter than    2936 bp
  85% of sequence in fragments shorter than    3349 bp
  90% of sequence in fragments shorter than    4001 bp
  95% of sequence in fragments shorter than    5248 bp

# BscGI.SspI (CCCGT+AATATT) double digest, 3607 thousand fragments (1231070+2377267 sites), 825 bp average fragment length
   5% of sequence in fragments shorter than     285 bp
  10% of sequence in fragments shorter than     413 bp
  15% of sequence in fragments shorter than     551 bp
  20% of sequence in fragments shorter than     682 bp
  25% of sequence in fragments shorter than     802 bp
  30% of sequence in fragments shorter than     929 bp
  35% of sequence in fragments shorter than    1058 bp
  40% of sequence in fragments shorter than    1194 bp
  45% of sequence in fragments shorter than    1336 bp
  50% of sequence in fragments shorter than    1487 bp
  55% of sequence in fragments shorter than    1649 bp
  60% of sequence in fragments shorter than    1827 bp
  65% of sequence in fragments shorter than    2022 bp
  70% of sequence in fragments shorter than    2247 bp
  75% of sequence in fragments shorter than    2504 bp
  80% of sequence in fragments shorter than    2820 bp
  85% of sequence in fragments shorter than    3219 bp
  90% of sequence in fragments shorter than    3786 bp
  95% of sequence in fragments shorter than    4696 bp

# AvaII.HindIII (GGWCC+AAGCTT) double digest, 3607 thousand fragments (2747690+860365 sites), 826 bp average fragment length
   5% of sequence in fragments shorter than     272 bp
  10% of sequence in fragments shorter than     422 bp
  15% of sequence in fragments shorter than     563 bp
  20% of sequence in fragments shorter than     694 bp
  25% of sequence in fragments shorter than     830 bp
  30% of sequence in fragments shorter than     965 bp
  35% of sequence in fragments shorter than    1104 bp
  40% of sequence in fragments shorter than    1253 bp
  45% of sequence in fragments shorter than    1411 bp
  50% of sequence in fragments shorter than    1580 bp
  55% of sequence in fragments shorter than    1757 bp
  60% of sequence in fragments shorter than    1955 bp
  65% of sequence in fragments shorter than    2177 bp
  70% of sequence in fragments shorter than    2427 bp
  75% of sequence in fragments shorter than    2720 bp
  80% of sequence in fragments shorter than    3075 bp
  85% of sequence in fragments shorter than    3531 bp
  90% of sequence in fragments shorter than    4181 bp
  95% of sequence in fragments shorter than    5329 bp

# AatII.SfeI (GACGTC+CTRYAG) double digest, 3601 thousand fragments (71089+3531009 sites), 827 bp average fragment length
   5% of sequence in fragments shorter than     290 bp
  10% of sequence in fragments shorter than     437 bp
  15% of sequence in fragments shorter than     561 bp
  20% of sequence in fragments shorter than     681 bp
  25% of sequence in fragments shorter than     799 bp
  30% of sequence in fragments shorter than     919 bp
  35% of sequence in fragments shorter than    1038 bp
  40% of sequence in fragments shorter than    1161 bp
  45% of sequence in fragments shorter than    1292 bp
  50% of sequence in fragments shorter than    1425 bp
  55% of sequence in fragments shorter than    1574 bp
  60% of sequence in fragments shorter than    1734 bp
  65% of sequence in fragments shorter than    1910 bp
  70% of sequence in fragments shorter than    2108 bp
  75% of sequence in fragments shorter than    2319 bp
  80% of sequence in fragments shorter than    2597 bp
  85% of sequence in fragments shorter than    2951 bp
  90% of sequence in fragments shorter than    3450 bp
  95% of sequence in fragments shorter than    4323 bp

# ApaLI.NspI (GTGCAC+RCATGY) double digest, 3600 thousand fragments (496255+3104359 sites), 827 bp average fragment length
   5% of sequence in fragments shorter than     293 bp
  10% of sequence in fragments shorter than     446 bp
  15% of sequence in fragments shorter than     585 bp
  20% of sequence in fragments shorter than     713 bp
  25% of sequence in fragments shorter than     838 bp
  30% of sequence in fragments shorter than     963 bp
  35% of sequence in fragments shorter than    1089 bp
  40% of sequence in fragments shorter than    1219 bp
  45% of sequence in fragments shorter than    1358 bp
  50% of sequence in fragments shorter than    1503 bp
  55% of sequence in fragments shorter than    1660 bp
  60% of sequence in fragments shorter than    1829 bp
  65% of sequence in fragments shorter than    2017 bp
  70% of sequence in fragments shorter than    2227 bp
  75% of sequence in fragments shorter than    2479 bp
  80% of sequence in fragments shorter than    2781 bp
  85% of sequence in fragments shorter than    3167 bp
  90% of sequence in fragments shorter than    3706 bp
  95% of sequence in fragments shorter than    4766 bp

# AvaII.XbaI (GGWCC+TCTAGA) double digest, 3598 thousand fragments (2747690+850998 sites), 828 bp average fragment length
   5% of sequence in fragments shorter than     275 bp
  10% of sequence in fragments shorter than     425 bp
  15% of sequence in fragments shorter than     571 bp
  20% of sequence in fragments shorter than     705 bp
  25% of sequence in fragments shorter than     836 bp
  30% of sequence in fragments shorter than     971 bp
  35% of sequence in fragments shorter than    1110 bp
  40% of sequence in fragments shorter than    1248 bp
  45% of sequence in fragments shorter than    1400 bp
  50% of sequence in fragments shorter than    1565 bp
  55% of sequence in fragments shorter than    1738 bp
  60% of sequence in fragments shorter than    1930 bp
  65% of sequence in fragments shorter than    2144 bp
  70% of sequence in fragments shorter than    2392 bp
  75% of sequence in fragments shorter than    2669 bp
  80% of sequence in fragments shorter than    3015 bp
  85% of sequence in fragments shorter than    3453 bp
  90% of sequence in fragments shorter than    4071 bp
  95% of sequence in fragments shorter than    5134 bp

# SacII.SfeI (CCGCGG+CTRYAG) double digest, 3597 thousand fragments (66901+3531009 sites), 828 bp average fragment length
   5% of sequence in fragments shorter than     290 bp
  10% of sequence in fragments shorter than     438 bp
  15% of sequence in fragments shorter than     562 bp
  20% of sequence in fragments shorter than     683 bp
  25% of sequence in fragments shorter than     803 bp
  30% of sequence in fragments shorter than     923 bp
  35% of sequence in fragments shorter than    1043 bp
  40% of sequence in fragments shorter than    1167 bp
  45% of sequence in fragments shorter than    1299 bp
  50% of sequence in fragments shorter than    1434 bp
  55% of sequence in fragments shorter than    1583 bp
  60% of sequence in fragments shorter than    1746 bp
  65% of sequence in fragments shorter than    1923 bp
  70% of sequence in fragments shorter than    2121 bp
  75% of sequence in fragments shorter than    2335 bp
  80% of sequence in fragments shorter than    2617 bp
  85% of sequence in fragments shorter than    2975 bp
  90% of sequence in fragments shorter than    3479 bp
  95% of sequence in fragments shorter than    4361 bp

# AvaII.EcoRI (GGWCC+GAATTC) double digest, 3594 thousand fragments (2747690+847401 sites), 829 bp average fragment length
   5% of sequence in fragments shorter than     277 bp
  10% of sequence in fragments shorter than     423 bp
  15% of sequence in fragments shorter than     569 bp
  20% of sequence in fragments shorter than     704 bp
  25% of sequence in fragments shorter than     845 bp
  30% of sequence in fragments shorter than     972 bp
  35% of sequence in fragments shorter than    1119 bp
  40% of sequence in fragments shorter than    1273 bp
  45% of sequence in fragments shorter than    1430 bp
  50% of sequence in fragments shorter than    1602 bp
  55% of sequence in fragments shorter than    1782 bp
  60% of sequence in fragments shorter than    1982 bp
  65% of sequence in fragments shorter than    2208 bp
  70% of sequence in fragments shorter than    2461 bp
  75% of sequence in fragments shorter than    2750 bp
  80% of sequence in fragments shorter than    3107 bp
  85% of sequence in fragments shorter than    3561 bp
  90% of sequence in fragments shorter than    4211 bp
  95% of sequence in fragments shorter than    5343 bp

# PspXI.SfeI (VCTCGAGB+CTRYAG) double digest, 3589 thousand fragments (59193+3531009 sites), 830 bp average fragment length
   5% of sequence in fragments shorter than     291 bp
  10% of sequence in fragments shorter than     439 bp
  15% of sequence in fragments shorter than     563 bp
  20% of sequence in fragments shorter than     684 bp
  25% of sequence in fragments shorter than     803 bp
  30% of sequence in fragments shorter than     923 bp
  35% of sequence in fragments shorter than    1043 bp
  40% of sequence in fragments shorter than    1166 bp
  45% of sequence in fragments shorter than    1297 bp
  50% of sequence in fragments shorter than    1431 bp
  55% of sequence in fragments shorter than    1580 bp
  60% of sequence in fragments shorter than    1742 bp
  65% of sequence in fragments shorter than    1919 bp
  70% of sequence in fragments shorter than    2116 bp
  75% of sequence in fragments shorter than    2328 bp
  80% of sequence in fragments shorter than    2608 bp
  85% of sequence in fragments shorter than    2963 bp
  90% of sequence in fragments shorter than    3465 bp
  95% of sequence in fragments shorter than    4340 bp

# HindIII.SmlI (AAGCTT+CTYRAG) double digest, 3587 thousand fragments (860365+2727867 sites), 830 bp average fragment length
   5% of sequence in fragments shorter than     288 bp
  10% of sequence in fragments shorter than     440 bp
  15% of sequence in fragments shorter than     567 bp
  20% of sequence in fragments shorter than     688 bp
  25% of sequence in fragments shorter than     808 bp
  30% of sequence in fragments shorter than     928 bp
  35% of sequence in fragments shorter than    1048 bp
  40% of sequence in fragments shorter than    1174 bp
  45% of sequence in fragments shorter than    1308 bp
  50% of sequence in fragments shorter than    1444 bp
  55% of sequence in fragments shorter than    1596 bp
  60% of sequence in fragments shorter than    1760 bp
  65% of sequence in fragments shorter than    1926 bp
  70% of sequence in fragments shorter than    2121 bp
  75% of sequence in fragments shorter than    2353 bp
  80% of sequence in fragments shorter than    2630 bp
  85% of sequence in fragments shorter than    2986 bp
  90% of sequence in fragments shorter than    3496 bp
  95% of sequence in fragments shorter than    4405 bp

# AgeI.SfeI (ACCGGT+CTRYAG) double digest, 3583 thousand fragments (53386+3531009 sites), 831 bp average fragment length
   5% of sequence in fragments shorter than     292 bp
  10% of sequence in fragments shorter than     440 bp
  15% of sequence in fragments shorter than     563 bp
  20% of sequence in fragments shorter than     682 bp
  25% of sequence in fragments shorter than     801 bp
  30% of sequence in fragments shorter than     922 bp
  35% of sequence in fragments shorter than    1041 bp
  40% of sequence in fragments shorter than    1166 bp
  45% of sequence in fragments shorter than    1297 bp
  50% of sequence in fragments shorter than    1431 bp
  55% of sequence in fragments shorter than    1580 bp
  60% of sequence in fragments shorter than    1741 bp
  65% of sequence in fragments shorter than    1919 bp
  70% of sequence in fragments shorter than    2116 bp
  75% of sequence in fragments shorter than    2328 bp
  80% of sequence in fragments shorter than    2608 bp
  85% of sequence in fragments shorter than    2961 bp
  90% of sequence in fragments shorter than    3464 bp
  95% of sequence in fragments shorter than    4339 bp

# SmlI.XbaI (CTYRAG+TCTAGA) double digest, 3578 thousand fragments (2727867+850998 sites), 832 bp average fragment length
   5% of sequence in fragments shorter than     291 bp
  10% of sequence in fragments shorter than     441 bp
  15% of sequence in fragments shorter than     573 bp
  20% of sequence in fragments shorter than     691 bp
  25% of sequence in fragments shorter than     811 bp
  30% of sequence in fragments shorter than     921 bp
  35% of sequence in fragments shorter than    1037 bp
  40% of sequence in fragments shorter than    1159 bp
  45% of sequence in fragments shorter than    1288 bp
  50% of sequence in fragments shorter than    1417 bp
  55% of sequence in fragments shorter than    1564 bp
  60% of sequence in fragments shorter than    1722 bp
  65% of sequence in fragments shorter than    1886 bp
  70% of sequence in fragments shorter than    2080 bp
  75% of sequence in fragments shorter than    2310 bp
  80% of sequence in fragments shorter than    2586 bp
  85% of sequence in fragments shorter than    2929 bp
  90% of sequence in fragments shorter than    3420 bp
  95% of sequence in fragments shorter than    4260 bp

# EcoRI.SmlI (GAATTC+CTYRAG) double digest, 3574 thousand fragments (847401+2727867 sites), 833 bp average fragment length
   5% of sequence in fragments shorter than     290 bp
  10% of sequence in fragments shorter than     434 bp
  15% of sequence in fragments shorter than     565 bp
  20% of sequence in fragments shorter than     686 bp
  25% of sequence in fragments shorter than     797 bp
  30% of sequence in fragments shorter than     918 bp
  35% of sequence in fragments shorter than    1038 bp
  40% of sequence in fragments shorter than    1164 bp
  45% of sequence in fragments shorter than    1297 bp
  50% of sequence in fragments shorter than    1434 bp
  55% of sequence in fragments shorter than    1584 bp
  60% of sequence in fragments shorter than    1749 bp
  65% of sequence in fragments shorter than    1920 bp
  70% of sequence in fragments shorter than    2118 bp
  75% of sequence in fragments shorter than    2350 bp
  80% of sequence in fragments shorter than    2628 bp
  85% of sequence in fragments shorter than    2971 bp
  90% of sequence in fragments shorter than    3475 bp
  95% of sequence in fragments shorter than    4350 bp

# ApaI.StyI (GGGCCC+CCWWGG) double digest, 3573 thousand fragments (460339+3114107 sites), 833 bp average fragment length
   5% of sequence in fragments shorter than     277 bp
  10% of sequence in fragments shorter than     432 bp
  15% of sequence in fragments shorter than     569 bp
  20% of sequence in fragments shorter than     703 bp
  25% of sequence in fragments shorter than     829 bp
  30% of sequence in fragments shorter than     967 bp
  35% of sequence in fragments shorter than    1108 bp
  40% of sequence in fragments shorter than    1258 bp
  45% of sequence in fragments shorter than    1411 bp
  50% of sequence in fragments shorter than    1582 bp
  55% of sequence in fragments shorter than    1767 bp
  60% of sequence in fragments shorter than    1972 bp
  65% of sequence in fragments shorter than    2205 bp
  70% of sequence in fragments shorter than    2470 bp
  75% of sequence in fragments shorter than    2776 bp
  80% of sequence in fragments shorter than    3143 bp
  85% of sequence in fragments shorter than    3618 bp
  90% of sequence in fragments shorter than    4341 bp
  95% of sequence in fragments shorter than    5784 bp

# PmeI.SfeI (GTTTAAAC+CTRYAG) double digest, 3572 thousand fragments (42094+3531009 sites), 834 bp average fragment length
   5% of sequence in fragments shorter than     293 bp
  10% of sequence in fragments shorter than     441 bp
  15% of sequence in fragments shorter than     565 bp
  20% of sequence in fragments shorter than     685 bp
  25% of sequence in fragments shorter than     805 bp
  30% of sequence in fragments shorter than     926 bp
  35% of sequence in fragments shorter than    1045 bp
  40% of sequence in fragments shorter than    1168 bp
  45% of sequence in fragments shorter than    1300 bp
  50% of sequence in fragments shorter than    1433 bp
  55% of sequence in fragments shorter than    1582 bp
  60% of sequence in fragments shorter than    1743 bp
  65% of sequence in fragments shorter than    1919 bp
  70% of sequence in fragments shorter than    2116 bp
  75% of sequence in fragments shorter than    2328 bp
  80% of sequence in fragments shorter than    2606 bp
  85% of sequence in fragments shorter than    2960 bp
  90% of sequence in fragments shorter than    3459 bp
  95% of sequence in fragments shorter than    4330 bp

# ApaI.NspI (GGGCCC+RCATGY) double digest, 3564 thousand fragments (460339+3104359 sites), 836 bp average fragment length
   5% of sequence in fragments shorter than     289 bp
  10% of sequence in fragments shorter than     443 bp
  15% of sequence in fragments shorter than     582 bp
  20% of sequence in fragments shorter than     710 bp
  25% of sequence in fragments shorter than     836 bp
  30% of sequence in fragments shorter than     961 bp
  35% of sequence in fragments shorter than    1088 bp
  40% of sequence in fragments shorter than    1218 bp
  45% of sequence in fragments shorter than    1357 bp
  50% of sequence in fragments shorter than    1503 bp
  55% of sequence in fragments shorter than    1661 bp
  60% of sequence in fragments shorter than    1832 bp
  65% of sequence in fragments shorter than    2023 bp
  70% of sequence in fragments shorter than    2236 bp
  75% of sequence in fragments shorter than    2491 bp
  80% of sequence in fragments shorter than    2797 bp
  85% of sequence in fragments shorter than    3191 bp
  90% of sequence in fragments shorter than    3735 bp
  95% of sequence in fragments shorter than    4833 bp

# SalI.SfeI (GTCGAC+CTRYAG) double digest, 3561 thousand fragments (31396+3531009 sites), 836 bp average fragment length
   5% of sequence in fragments shorter than     294 bp
  10% of sequence in fragments shorter than     442 bp
  15% of sequence in fragments shorter than     567 bp
  20% of sequence in fragments shorter than     687 bp
  25% of sequence in fragments shorter than     809 bp
  30% of sequence in fragments shorter than     930 bp
  35% of sequence in fragments shorter than    1050 bp
  40% of sequence in fragments shorter than    1174 bp
  45% of sequence in fragments shorter than    1306 bp
  50% of sequence in fragments shorter than    1440 bp
  55% of sequence in fragments shorter than    1589 bp
  60% of sequence in fragments shorter than    1752 bp
  65% of sequence in fragments shorter than    1927 bp
  70% of sequence in fragments shorter than    2125 bp
  75% of sequence in fragments shorter than    2341 bp
  80% of sequence in fragments shorter than    2623 bp
  85% of sequence in fragments shorter than    2979 bp
  90% of sequence in fragments shorter than    3483 bp
  95% of sequence in fragments shorter than    4360 bp

# EcoRV.StyI (GATATC+CCWWGG) double digest, 3560 thousand fragments (446473+3114107 sites), 837 bp average fragment length
   5% of sequence in fragments shorter than     288 bp
  10% of sequence in fragments shorter than     438 bp
  15% of sequence in fragments shorter than     568 bp
  20% of sequence in fragments shorter than     693 bp
  25% of sequence in fragments shorter than     808 bp
  30% of sequence in fragments shorter than     933 bp
  35% of sequence in fragments shorter than    1058 bp
  40% of sequence in fragments shorter than    1183 bp
  45% of sequence in fragments shorter than    1318 bp
  50% of sequence in fragments shorter than    1461 bp
  55% of sequence in fragments shorter than    1621 bp
  60% of sequence in fragments shorter than    1790 bp
  65% of sequence in fragments shorter than    1984 bp
  70% of sequence in fragments shorter than    2196 bp
  75% of sequence in fragments shorter than    2452 bp
  80% of sequence in fragments shorter than    2763 bp
  85% of sequence in fragments shorter than    3163 bp
  90% of sequence in fragments shorter than    3743 bp
  95% of sequence in fragments shorter than    4829 bp

# MluI.SfeI (ACGCGT+CTRYAG) double digest, 3551 thousand fragments (21415+3531009 sites), 838 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     443 bp
  15% of sequence in fragments shorter than     569 bp
  20% of sequence in fragments shorter than     689 bp
  25% of sequence in fragments shorter than     811 bp
  30% of sequence in fragments shorter than     932 bp
  35% of sequence in fragments shorter than    1053 bp
  40% of sequence in fragments shorter than    1176 bp
  45% of sequence in fragments shorter than    1310 bp
  50% of sequence in fragments shorter than    1444 bp
  55% of sequence in fragments shorter than    1593 bp
  60% of sequence in fragments shorter than    1756 bp
  65% of sequence in fragments shorter than    1932 bp
  70% of sequence in fragments shorter than    2128 bp
  75% of sequence in fragments shorter than    2348 bp
  80% of sequence in fragments shorter than    2630 bp
  85% of sequence in fragments shorter than    2988 bp
  90% of sequence in fragments shorter than    3493 bp
  95% of sequence in fragments shorter than    4377 bp

# EcoRV.NspI (GATATC+RCATGY) double digest, 3550 thousand fragments (446473+3104359 sites), 839 bp average fragment length
   5% of sequence in fragments shorter than     292 bp
  10% of sequence in fragments shorter than     447 bp
  15% of sequence in fragments shorter than     585 bp
  20% of sequence in fragments shorter than     712 bp
  25% of sequence in fragments shorter than     836 bp
  30% of sequence in fragments shorter than     961 bp
  35% of sequence in fragments shorter than    1085 bp
  40% of sequence in fragments shorter than    1216 bp
  45% of sequence in fragments shorter than    1354 bp
  50% of sequence in fragments shorter than    1498 bp
  55% of sequence in fragments shorter than    1653 bp
  60% of sequence in fragments shorter than    1819 bp
  65% of sequence in fragments shorter than    2005 bp
  70% of sequence in fragments shorter than    2209 bp
  75% of sequence in fragments shorter than    2432 bp
  80% of sequence in fragments shorter than    2725 bp
  85% of sequence in fragments shorter than    3099 bp
  90% of sequence in fragments shorter than    3624 bp
  95% of sequence in fragments shorter than    4589 bp

# BscGI.HpaII (CCCGT+CCGG) double digest, 3548 thousand fragments (1231070+2317722 sites), 839 bp average fragment length
   5% of sequence in fragments shorter than     252 bp
  10% of sequence in fragments shorter than     429 bp
  15% of sequence in fragments shorter than     632 bp
  20% of sequence in fragments shorter than     829 bp
  25% of sequence in fragments shorter than    1037 bp
  30% of sequence in fragments shorter than    1261 bp
  35% of sequence in fragments shorter than    1499 bp
  40% of sequence in fragments shorter than    1755 bp
  45% of sequence in fragments shorter than    2033 bp
  50% of sequence in fragments shorter than    2341 bp
  55% of sequence in fragments shorter than    2681 bp
  60% of sequence in fragments shorter than    3064 bp
  65% of sequence in fragments shorter than    3493 bp
  70% of sequence in fragments shorter than    3990 bp
  75% of sequence in fragments shorter than    4571 bp
  80% of sequence in fragments shorter than    5289 bp
  85% of sequence in fragments shorter than    6217 bp
  90% of sequence in fragments shorter than    7518 bp
  95% of sequence in fragments shorter than    9815 bp

# SfeI.SgrAI (CTRYAG+CRCCGGYG) double digest, 3545 thousand fragments (3531009+15270 sites), 840 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     444 bp
  15% of sequence in fragments shorter than     570 bp
  20% of sequence in fragments shorter than     690 bp
  25% of sequence in fragments shorter than     812 bp
  30% of sequence in fragments shorter than     934 bp
  35% of sequence in fragments shorter than    1055 bp
  40% of sequence in fragments shorter than    1179 bp
  45% of sequence in fragments shorter than    1312 bp
  50% of sequence in fragments shorter than    1447 bp
  55% of sequence in fragments shorter than    1596 bp
  60% of sequence in fragments shorter than    1760 bp
  65% of sequence in fragments shorter than    1936 bp
  70% of sequence in fragments shorter than    2130 bp
  75% of sequence in fragments shorter than    2353 bp
  80% of sequence in fragments shorter than    2635 bp
  85% of sequence in fragments shorter than    2993 bp
  90% of sequence in fragments shorter than    3500 bp
  95% of sequence in fragments shorter than    4385 bp

# NruI.SfeI (TCGCGA+CTRYAG) double digest, 3545 thousand fragments (14872+3531009 sites), 840 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     444 bp
  15% of sequence in fragments shorter than     570 bp
  20% of sequence in fragments shorter than     690 bp
  25% of sequence in fragments shorter than     812 bp
  30% of sequence in fragments shorter than     934 bp
  35% of sequence in fragments shorter than    1054 bp
  40% of sequence in fragments shorter than    1179 bp
  45% of sequence in fragments shorter than    1312 bp
  50% of sequence in fragments shorter than    1447 bp
  55% of sequence in fragments shorter than    1596 bp
  60% of sequence in fragments shorter than    1760 bp
  65% of sequence in fragments shorter than    1936 bp
  70% of sequence in fragments shorter than    2130 bp
  75% of sequence in fragments shorter than    2353 bp
  80% of sequence in fragments shorter than    2635 bp
  85% of sequence in fragments shorter than    2993 bp
  90% of sequence in fragments shorter than    3499 bp
  95% of sequence in fragments shorter than    4383 bp

# PvuI.SfeI (CGATCG+CTRYAG) double digest, 3544 thousand fragments (14186+3531009 sites), 840 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     445 bp
  15% of sequence in fragments shorter than     570 bp
  20% of sequence in fragments shorter than     690 bp
  25% of sequence in fragments shorter than     813 bp
  30% of sequence in fragments shorter than     934 bp
  35% of sequence in fragments shorter than    1055 bp
  40% of sequence in fragments shorter than    1180 bp
  45% of sequence in fragments shorter than    1313 bp
  50% of sequence in fragments shorter than    1447 bp
  55% of sequence in fragments shorter than    1597 bp
  60% of sequence in fragments shorter than    1759 bp
  65% of sequence in fragments shorter than    1934 bp
  70% of sequence in fragments shorter than    2129 bp
  75% of sequence in fragments shorter than    2350 bp
  80% of sequence in fragments shorter than    2632 bp
  85% of sequence in fragments shorter than    2989 bp
  90% of sequence in fragments shorter than    3492 bp
  95% of sequence in fragments shorter than    4367 bp

# FseI.SfeI (GGCCGGCC+CTRYAG) double digest, 3544 thousand fragments (13662+3531009 sites), 840 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     445 bp
  15% of sequence in fragments shorter than     570 bp
  20% of sequence in fragments shorter than     690 bp
  25% of sequence in fragments shorter than     813 bp
  30% of sequence in fragments shorter than     934 bp
  35% of sequence in fragments shorter than    1055 bp
  40% of sequence in fragments shorter than    1180 bp
  45% of sequence in fragments shorter than    1313 bp
  50% of sequence in fragments shorter than    1448 bp
  55% of sequence in fragments shorter than    1597 bp
  60% of sequence in fragments shorter than    1761 bp
  65% of sequence in fragments shorter than    1937 bp
  70% of sequence in fragments shorter than    2130 bp
  75% of sequence in fragments shorter than    2354 bp
  80% of sequence in fragments shorter than    2637 bp
  85% of sequence in fragments shorter than    2995 bp
  90% of sequence in fragments shorter than    3502 bp
  95% of sequence in fragments shorter than    4387 bp

# NotI.SfeI (GCGGCCGC+CTRYAG) double digest, 3540 thousand fragments (9795+3531009 sites), 841 bp average fragment length
   5% of sequence in fragments shorter than     296 bp
  10% of sequence in fragments shorter than     445 bp
  15% of sequence in fragments shorter than     571 bp
  20% of sequence in fragments shorter than     691 bp
  25% of sequence in fragments shorter than     814 bp
  30% of sequence in fragments shorter than     935 bp
  35% of sequence in fragments shorter than    1056 bp
  40% of sequence in fragments shorter than    1181 bp
  45% of sequence in fragments shorter than    1314 bp
  50% of sequence in fragments shorter than    1449 bp
  55% of sequence in fragments shorter than    1599 bp
  60% of sequence in fragments shorter than    1762 bp
  65% of sequence in fragments shorter than    1938 bp
  70% of sequence in fragments shorter than    2131 bp
  75% of sequence in fragments shorter than    2356 bp
  80% of sequence in fragments shorter than    2639 bp
  85% of sequence in fragments shorter than    2998 bp
  90% of sequence in fragments shorter than    3504 bp
  95% of sequence in fragments shorter than    4389 bp

# RsrII.SfeI (CGGWCCG+CTRYAG) double digest, 3540 thousand fragments (9764+3531009 sites), 841 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     445 bp
  15% of sequence in fragments shorter than     571 bp
  20% of sequence in fragments shorter than     691 bp
  25% of sequence in fragments shorter than     814 bp
  30% of sequence in fragments shorter than     935 bp
  35% of sequence in fragments shorter than    1056 bp
  40% of sequence in fragments shorter than    1181 bp
  45% of sequence in fragments shorter than    1314 bp
  50% of sequence in fragments shorter than    1449 bp
  55% of sequence in fragments shorter than    1598 bp
  60% of sequence in fragments shorter than    1762 bp
  65% of sequence in fragments shorter than    1938 bp
  70% of sequence in fragments shorter than    2131 bp
  75% of sequence in fragments shorter than    2355 bp
  80% of sequence in fragments shorter than    2637 bp
  85% of sequence in fragments shorter than    2996 bp
  90% of sequence in fragments shorter than    3502 bp
  95% of sequence in fragments shorter than    4388 bp

# AscI.SfeI (GGCGCGCC+CTRYAG) double digest, 3535 thousand fragments (4751+3531009 sites), 842 bp average fragment length
   5% of sequence in fragments shorter than     296 bp
  10% of sequence in fragments shorter than     446 bp
  15% of sequence in fragments shorter than     572 bp
  20% of sequence in fragments shorter than     692 bp
  25% of sequence in fragments shorter than     815 bp
  30% of sequence in fragments shorter than     937 bp
  35% of sequence in fragments shorter than    1057 bp
  40% of sequence in fragments shorter than    1182 bp
  45% of sequence in fragments shorter than    1316 bp
  50% of sequence in fragments shorter than    1451 bp
  55% of sequence in fragments shorter than    1600 bp
  60% of sequence in fragments shorter than    1764 bp
  65% of sequence in fragments shorter than    1940 bp
  70% of sequence in fragments shorter than    2132 bp
  75% of sequence in fragments shorter than    2359 bp
  80% of sequence in fragments shorter than    2641 bp
  85% of sequence in fragments shorter than    3001 bp
  90% of sequence in fragments shorter than    3508 bp
  95% of sequence in fragments shorter than    4393 bp

# PstI.SfeI (CTGCAG+CTRYAG) double digest, 3530 thousand fragments (1321469+3531009 sites), 844 bp average fragment length
   5% of sequence in fragments shorter than     296 bp
  10% of sequence in fragments shorter than     446 bp
  15% of sequence in fragments shorter than     573 bp
  20% of sequence in fragments shorter than     693 bp
  25% of sequence in fragments shorter than     816 bp
  30% of sequence in fragments shorter than     938 bp
  35% of sequence in fragments shorter than    1059 bp
  40% of sequence in fragments shorter than    1184 bp
  45% of sequence in fragments shorter than    1318 bp
  50% of sequence in fragments shorter than    1453 bp
  55% of sequence in fragments shorter than    1602 bp
  60% of sequence in fragments shorter than    1766 bp
  65% of sequence in fragments shorter than    1943 bp
  70% of sequence in fragments shorter than    2132 bp
  75% of sequence in fragments shorter than    2362 bp
  80% of sequence in fragments shorter than    2645 bp
  85% of sequence in fragments shorter than    3004 bp
  90% of sequence in fragments shorter than    3512 bp
  95% of sequence in fragments shorter than    4398 bp

# AvaII.BglII (GGWCC+AGATCT) double digest, 3521 thousand fragments (2747690+774732 sites), 846 bp average fragment length
   5% of sequence in fragments shorter than     282 bp
  10% of sequence in fragments shorter than     433 bp
  15% of sequence in fragments shorter than     576 bp
  20% of sequence in fragments shorter than     716 bp
  25% of sequence in fragments shorter than     859 bp
  30% of sequence in fragments shorter than     990 bp
  35% of sequence in fragments shorter than    1139 bp
  40% of sequence in fragments shorter than    1293 bp
  45% of sequence in fragments shorter than    1450 bp
  50% of sequence in fragments shorter than    1625 bp
  55% of sequence in fragments shorter than    1813 bp
  60% of sequence in fragments shorter than    2016 bp
  65% of sequence in fragments shorter than    2245 bp
  70% of sequence in fragments shorter than    2508 bp
  75% of sequence in fragments shorter than    2815 bp
  80% of sequence in fragments shorter than    3190 bp
  85% of sequence in fragments shorter than    3673 bp
  90% of sequence in fragments shorter than    4363 bp
  95% of sequence in fragments shorter than    5645 bp

# AflIII.NspI (ACRYGT+RCATGY) double digest, 3521 thousand fragments (1485394+3104359 sites), 846 bp average fragment length
   5% of sequence in fragments shorter than     299 bp
  10% of sequence in fragments shorter than     459 bp
  15% of sequence in fragments shorter than     602 bp
  20% of sequence in fragments shorter than     734 bp
  25% of sequence in fragments shorter than     862 bp
  30% of sequence in fragments shorter than     992 bp
  35% of sequence in fragments shorter than    1122 bp
  40% of sequence in fragments shorter than    1255 bp
  45% of sequence in fragments shorter than    1397 bp
  50% of sequence in fragments shorter than    1547 bp
  55% of sequence in fragments shorter than    1707 bp
  60% of sequence in fragments shorter than    1882 bp
  65% of sequence in fragments shorter than    2075 bp
  70% of sequence in fragments shorter than    2297 bp
  75% of sequence in fragments shorter than    2557 bp
  80% of sequence in fragments shorter than    2861 bp
  85% of sequence in fragments shorter than    3257 bp
  90% of sequence in fragments shorter than    3801 bp
  95% of sequence in fragments shorter than    4867 bp

# SpeI.StyI (ACTAGT+CCWWGG) double digest, 3513 thousand fragments (400286+3114107 sites), 848 bp average fragment length
   5% of sequence in fragments shorter than     286 bp
  10% of sequence in fragments shorter than     440 bp
  15% of sequence in fragments shorter than     568 bp
  20% of sequence in fragments shorter than     696 bp
  25% of sequence in fragments shorter than     820 bp
  30% of sequence in fragments shorter than     949 bp
  35% of sequence in fragments shorter than    1078 bp
  40% of sequence in fragments shorter than    1213 bp
  45% of sequence in fragments shorter than    1356 bp
  50% of sequence in fragments shorter than    1503 bp
  55% of sequence in fragments shorter than    1668 bp
  60% of sequence in fragments shorter than    1847 bp
  65% of sequence in fragments shorter than    2051 bp
  70% of sequence in fragments shorter than    2278 bp
  75% of sequence in fragments shorter than    2545 bp
  80% of sequence in fragments shorter than    2866 bp
  85% of sequence in fragments shorter than    3256 bp
  90% of sequence in fragments shorter than    3850 bp
  95% of sequence in fragments shorter than    4984 bp

# AvaII.NcoI (GGWCC+CCATGG) double digest, 3506 thousand fragments (2747690+759594 sites), 849 bp average fragment length
   5% of sequence in fragments shorter than     279 bp
  10% of sequence in fragments shorter than     439 bp
  15% of sequence in fragments shorter than     594 bp
  20% of sequence in fragments shorter than     741 bp
  25% of sequence in fragments shorter than     891 bp
  30% of sequence in fragments shorter than    1034 bp
  35% of sequence in fragments shorter than    1195 bp
  40% of sequence in fragments shorter than    1361 bp
  45% of sequence in fragments shorter than    1523 bp
  50% of sequence in fragments shorter than    1715 bp
  55% of sequence in fragments shorter than    1917 bp
  60% of sequence in fragments shorter than    2146 bp
  65% of sequence in fragments shorter than    2396 bp
  70% of sequence in fragments shorter than    2686 bp
  75% of sequence in fragments shorter than    3033 bp
  80% of sequence in fragments shorter than    3456 bp
  85% of sequence in fragments shorter than    3998 bp
  90% of sequence in fragments shorter than    4794 bp
  95% of sequence in fragments shorter than    6257 bp

# NspI.SpeI (RCATGY+ACTAGT) double digest, 3504 thousand fragments (3104359+400286 sites), 850 bp average fragment length
   5% of sequence in fragments shorter than     294 bp
  10% of sequence in fragments shorter than     452 bp
  15% of sequence in fragments shorter than     592 bp
  20% of sequence in fragments shorter than     723 bp
  25% of sequence in fragments shorter than     850 bp
  30% of sequence in fragments shorter than     979 bp
  35% of sequence in fragments shorter than    1107 bp
  40% of sequence in fragments shorter than    1240 bp
  45% of sequence in fragments shorter than    1380 bp
  50% of sequence in fragments shorter than    1529 bp
  55% of sequence in fragments shorter than    1688 bp
  60% of sequence in fragments shorter than    1861 bp
  65% of sequence in fragments shorter than    2055 bp
  70% of sequence in fragments shorter than    2266 bp
  75% of sequence in fragments shorter than    2524 bp
  80% of sequence in fragments shorter than    2832 bp
  85% of sequence in fragments shorter than    3229 bp
  90% of sequence in fragments shorter than    3772 bp
  95% of sequence in fragments shorter than    4850 bp

# HpaI.StyI (GTTAAC+CCWWGG) double digest, 3503 thousand fragments (389418+3114107 sites), 850 bp average fragment length
   5% of sequence in fragments shorter than     291 bp
  10% of sequence in fragments shorter than     444 bp
  15% of sequence in fragments shorter than     577 bp
  20% of sequence in fragments shorter than     704 bp
  25% of sequence in fragments shorter than     821 bp
  30% of sequence in fragments shorter than     948 bp
  35% of sequence in fragments shorter than    1078 bp
  40% of sequence in fragments shorter than    1214 bp
  45% of sequence in fragments shorter than    1352 bp
  50% of sequence in fragments shorter than    1499 bp
  55% of sequence in fragments shorter than    1664 bp
  60% of sequence in fragments shorter than    1841 bp
  65% of sequence in fragments shorter than    2037 bp
  70% of sequence in fragments shorter than    2267 bp
  75% of sequence in fragments shorter than    2536 bp
  80% of sequence in fragments shorter than    2862 bp
  85% of sequence in fragments shorter than    3273 bp
  90% of sequence in fragments shorter than    3891 bp
  95% of sequence in fragments shorter than    5095 bp

# BglII.SmlI (AGATCT+CTYRAG) double digest, 3502 thousand fragments (774732+2727867 sites), 850 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     450 bp
  15% of sequence in fragments shorter than     581 bp
  20% of sequence in fragments shorter than     700 bp
  25% of sequence in fragments shorter than     822 bp
  30% of sequence in fragments shorter than     944 bp
  35% of sequence in fragments shorter than    1065 bp
  40% of sequence in fragments shorter than    1193 bp
  45% of sequence in fragments shorter than    1322 bp
  50% of sequence in fragments shorter than    1458 bp
  55% of sequence in fragments shorter than    1612 bp
  60% of sequence in fragments shorter than    1777 bp
  65% of sequence in fragments shorter than    1960 bp
  70% of sequence in fragments shorter than    2154 bp
  75% of sequence in fragments shorter than    2395 bp
  80% of sequence in fragments shorter than    2683 bp
  85% of sequence in fragments shorter than    3057 bp
  90% of sequence in fragments shorter than    3597 bp
  95% of sequence in fragments shorter than    4607 bp

# HpaI.NspI (GTTAAC+RCATGY) double digest, 3493 thousand fragments (389418+3104359 sites), 853 bp average fragment length
   5% of sequence in fragments shorter than     297 bp
  10% of sequence in fragments shorter than     454 bp
  15% of sequence in fragments shorter than     595 bp
  20% of sequence in fragments shorter than     726 bp
  25% of sequence in fragments shorter than     854 bp
  30% of sequence in fragments shorter than     982 bp
  35% of sequence in fragments shorter than    1111 bp
  40% of sequence in fragments shorter than    1244 bp
  45% of sequence in fragments shorter than    1385 bp
  50% of sequence in fragments shorter than    1534 bp
  55% of sequence in fragments shorter than    1694 bp
  60% of sequence in fragments shorter than    1869 bp
  65% of sequence in fragments shorter than    2063 bp
  70% of sequence in fragments shorter than    2277 bp
  75% of sequence in fragments shorter than    2534 bp
  80% of sequence in fragments shorter than    2842 bp
  85% of sequence in fragments shorter than    3224 bp
  90% of sequence in fragments shorter than    3785 bp
  95% of sequence in fragments shorter than    4899 bp

# SapI.StyI (GCTCTTC+CCWWGG) double digest, 3490 thousand fragments (377161+3114107 sites), 853 bp average fragment length
   5% of sequence in fragments shorter than     289 bp
  10% of sequence in fragments shorter than     444 bp
  15% of sequence in fragments shorter than     579 bp
  20% of sequence in fragments shorter than     710 bp
  25% of sequence in fragments shorter than     827 bp
  30% of sequence in fragments shorter than     960 bp
  35% of sequence in fragments shorter than    1095 bp
  40% of sequence in fragments shorter than    1235 bp
  45% of sequence in fragments shorter than    1382 bp
  50% of sequence in fragments shorter than    1536 bp
  55% of sequence in fragments shorter than    1709 bp
  60% of sequence in fragments shorter than    1899 bp
  65% of sequence in fragments shorter than    2099 bp
  70% of sequence in fragments shorter than    2340 bp
  75% of sequence in fragments shorter than    2625 bp
  80% of sequence in fragments shorter than    2965 bp
  85% of sequence in fragments shorter than    3418 bp
  90% of sequence in fragments shorter than    4085 bp
  95% of sequence in fragments shorter than    5400 bp

# SmaI.StyI (CCCGGG+CCWWGG) double digest, 3490 thousand fragments (376940+3114107 sites), 853 bp average fragment length
   5% of sequence in fragments shorter than     282 bp
  10% of sequence in fragments shorter than     438 bp
  15% of sequence in fragments shorter than     573 bp
  20% of sequence in fragments shorter than     705 bp
  25% of sequence in fragments shorter than     828 bp
  30% of sequence in fragments shorter than     964 bp
  35% of sequence in fragments shorter than    1102 bp
  40% of sequence in fragments shorter than    1247 bp
  45% of sequence in fragments shorter than    1397 bp
  50% of sequence in fragments shorter than    1563 bp
  55% of sequence in fragments shorter than    1744 bp
  60% of sequence in fragments shorter than    1944 bp
  65% of sequence in fragments shorter than    2173 bp
  70% of sequence in fragments shorter than    2431 bp
  75% of sequence in fragments shorter than    2739 bp
  80% of sequence in fragments shorter than    3110 bp
  85% of sequence in fragments shorter than    3563 bp
  90% of sequence in fragments shorter than    4271 bp
  95% of sequence in fragments shorter than    5688 bp

# NcoI.SmlI (CCATGG+CTYRAG) double digest, 3486 thousand fragments (759594+2727867 sites), 854 bp average fragment length
   5% of sequence in fragments shorter than     291 bp
  10% of sequence in fragments shorter than     446 bp
  15% of sequence in fragments shorter than     578 bp
  20% of sequence in fragments shorter than     700 bp
  25% of sequence in fragments shorter than     822 bp
  30% of sequence in fragments shorter than     946 bp
  35% of sequence in fragments shorter than    1069 bp
  40% of sequence in fragments shorter than    1198 bp
  45% of sequence in fragments shorter than    1333 bp
  50% of sequence in fragments shorter than    1468 bp
  55% of sequence in fragments shorter than    1619 bp
  60% of sequence in fragments shorter than    1789 bp
  65% of sequence in fragments shorter than    1976 bp
  70% of sequence in fragments shorter than    2183 bp
  75% of sequence in fragments shorter than    2431 bp
  80% of sequence in fragments shorter than    2705 bp
  85% of sequence in fragments shorter than    3076 bp
  90% of sequence in fragments shorter than    3618 bp
  95% of sequence in fragments shorter than    4608 bp

# AvaII.BclI (GGWCC+TGATCA) double digest, 3486 thousand fragments (2747690+738785 sites), 854 bp average fragment length
   5% of sequence in fragments shorter than     281 bp
  10% of sequence in fragments shorter than     436 bp
  15% of sequence in fragments shorter than     584 bp
  20% of sequence in fragments shorter than     720 bp
  25% of sequence in fragments shorter than     864 bp
  30% of sequence in fragments shorter than    1001 bp
  35% of sequence in fragments shorter than    1148 bp
  40% of sequence in fragments shorter than    1300 bp
  45% of sequence in fragments shorter than    1461 bp
  50% of sequence in fragments shorter than    1635 bp
  55% of sequence in fragments shorter than    1820 bp
  60% of sequence in fragments shorter than    2022 bp
  65% of sequence in fragments shorter than    2250 bp
  70% of sequence in fragments shorter than    2507 bp
  75% of sequence in fragments shorter than    2803 bp
  80% of sequence in fragments shorter than    3170 bp
  85% of sequence in fragments shorter than    3644 bp
  90% of sequence in fragments shorter than    4333 bp
  95% of sequence in fragments shorter than    5598 bp

# AvaII.FnuDII (GGWCC+CGCG) double digest, 3481 thousand fragments (2747690+733940 sites), 856 bp average fragment length
   5% of sequence in fragments shorter than     280 bp
  10% of sequence in fragments shorter than     457 bp
  15% of sequence in fragments shorter than     630 bp
  20% of sequence in fragments shorter than     795 bp
  25% of sequence in fragments shorter than     950 bp
  30% of sequence in fragments shorter than    1124 bp
  35% of sequence in fragments shorter than    1306 bp
  40% of sequence in fragments shorter than    1495 bp
  45% of sequence in fragments shorter than    1700 bp
  50% of sequence in fragments shorter than    1909 bp
  55% of sequence in fragments shorter than    2145 bp
  60% of sequence in fragments shorter than    2403 bp
  65% of sequence in fragments shorter than    2688 bp
  70% of sequence in fragments shorter than    3023 bp
  75% of sequence in fragments shorter than    3420 bp
  80% of sequence in fragments shorter than    3905 bp
  85% of sequence in fragments shorter than    4537 bp
  90% of sequence in fragments shorter than    5445 bp
  95% of sequence in fragments shorter than    7144 bp

# NspI.SapI (RCATGY+GCTCTTC) double digest, 3481 thousand fragments (3104359+377161 sites), 856 bp average fragment length
   5% of sequence in fragments shorter than     300 bp
  10% of sequence in fragments shorter than     455 bp
  15% of sequence in fragments shorter than     596 bp
  20% of sequence in fragments shorter than     727 bp
  25% of sequence in fragments shorter than     853 bp
  30% of sequence in fragments shorter than     982 bp
  35% of sequence in fragments shorter than    1110 bp
  40% of sequence in fragments shorter than    1242 bp
  45% of sequence in fragments shorter than    1381 bp
  50% of sequence in fragments shorter than    1530 bp
  55% of sequence in fragments shorter than    1688 bp
  60% of sequence in fragments shorter than    1859 bp
  65% of sequence in fragments shorter than    2049 bp
  70% of sequence in fragments shorter than    2259 bp
  75% of sequence in fragments shorter than    2511 bp
  80% of sequence in fragments shorter than    2810 bp
  85% of sequence in fragments shorter than    3200 bp
  90% of sequence in fragments shorter than    3699 bp
  95% of sequence in fragments shorter than    4687 bp

# NspI.SmaI (RCATGY+CCCGGG) double digest, 3480 thousand fragments (3104359+376940 sites), 856 bp average fragment length
   5% of sequence in fragments shorter than     300 bp
  10% of sequence in fragments shorter than     456 bp
  15% of sequence in fragments shorter than     597 bp
  20% of sequence in fragments shorter than     726 bp
  25% of sequence in fragments shorter than     853 bp
  30% of sequence in fragments shorter than     981 bp
  35% of sequence in fragments shorter than    1108 bp
  40% of sequence in fragments shorter than    1240 bp
  45% of sequence in fragments shorter than    1380 bp
  50% of sequence in fragments shorter than    1528 bp
  55% of sequence in fragments shorter than    1687 bp
  60% of sequence in fragments shorter than    1857 bp
  65% of sequence in fragments shorter than    2048 bp
  70% of sequence in fragments shorter than    2260 bp
  75% of sequence in fragments shorter than    2516 bp
  80% of sequence in fragments shorter than    2822 bp
  85% of sequence in fragments shorter than    3216 bp
  90% of sequence in fragments shorter than    3754 bp
  95% of sequence in fragments shorter than    4847 bp

# BamHI.StyI (GGATCC+CCWWGG) double digest, 3479 thousand fragments (365999+3114107 sites), 856 bp average fragment length
   5% of sequence in fragments shorter than     289 bp
  10% of sequence in fragments shorter than     444 bp
  15% of sequence in fragments shorter than     584 bp
  20% of sequence in fragments shorter than     717 bp
  25% of sequence in fragments shorter than     841 bp
  30% of sequence in fragments shorter than     975 bp
  35% of sequence in fragments shorter than    1113 bp
  40% of sequence in fragments shorter than    1258 bp
  45% of sequence in fragments shorter than    1407 bp
  50% of sequence in fragments shorter than    1571 bp
  55% of sequence in fragments shorter than    1750 bp
  60% of sequence in fragments shorter than    1947 bp
  65% of sequence in fragments shorter than    2165 bp
  70% of sequence in fragments shorter than    2419 bp
  75% of sequence in fragments shorter than    2719 bp
  80% of sequence in fragments shorter than    3078 bp
  85% of sequence in fragments shorter than    3513 bp
  90% of sequence in fragments shorter than    4197 bp
  95% of sequence in fragments shorter than    5515 bp

# AccI.SspI (GTMKAC+AATATT) double digest, 3477 thousand fragments (1101063+2377267 sites), 856 bp average fragment length
   5% of sequence in fragments shorter than     285 bp
  10% of sequence in fragments shorter than     443 bp
  15% of sequence in fragments shorter than     582 bp
  20% of sequence in fragments shorter than     718 bp
  25% of sequence in fragments shorter than     853 bp
  30% of sequence in fragments shorter than     992 bp
  35% of sequence in fragments shorter than    1131 bp
  40% of sequence in fragments shorter than    1280 bp
  45% of sequence in fragments shorter than    1438 bp
  50% of sequence in fragments shorter than    1606 bp
  55% of sequence in fragments shorter than    1796 bp
  60% of sequence in fragments shorter than    2007 bp
  65% of sequence in fragments shorter than    2250 bp
  70% of sequence in fragments shorter than    2534 bp
  75% of sequence in fragments shorter than    2876 bp
  80% of sequence in fragments shorter than    3314 bp
  85% of sequence in fragments shorter than    3885 bp
  90% of sequence in fragments shorter than    4715 bp
  95% of sequence in fragments shorter than    6514 bp

# BamHI.NspI (GGATCC+RCATGY) double digest, 3469 thousand fragments (365999+3104359 sites), 858 bp average fragment length
   5% of sequence in fragments shorter than     301 bp
  10% of sequence in fragments shorter than     459 bp
  15% of sequence in fragments shorter than     602 bp
  20% of sequence in fragments shorter than     732 bp
  25% of sequence in fragments shorter than     853 bp
  30% of sequence in fragments shorter than     981 bp
  35% of sequence in fragments shorter than    1109 bp
  40% of sequence in fragments shorter than    1242 bp
  45% of sequence in fragments shorter than    1380 bp
  50% of sequence in fragments shorter than    1527 bp
  55% of sequence in fragments shorter than    1685 bp
  60% of sequence in fragments shorter than    1858 bp
  65% of sequence in fragments shorter than    2049 bp
  70% of sequence in fragments shorter than    2266 bp
  75% of sequence in fragments shorter than    2521 bp
  80% of sequence in fragments shorter than    2820 bp
  85% of sequence in fragments shorter than    3203 bp
  90% of sequence in fragments shorter than    3759 bp
  95% of sequence in fragments shorter than    4814 bp

# BclI.SmlI (TGATCA+CTYRAG) double digest, 3466 thousand fragments (738785+2727867 sites), 859 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     453 bp
  15% of sequence in fragments shorter than     585 bp
  20% of sequence in fragments shorter than     710 bp
  25% of sequence in fragments shorter than     833 bp
  30% of sequence in fragments shorter than     955 bp
  35% of sequence in fragments shorter than    1079 bp
  40% of sequence in fragments shorter than    1209 bp
  45% of sequence in fragments shorter than    1344 bp
  50% of sequence in fragments shorter than    1487 bp
  55% of sequence in fragments shorter than    1645 bp
  60% of sequence in fragments shorter than    1814 bp
  65% of sequence in fragments shorter than    2001 bp
  70% of sequence in fragments shorter than    2192 bp
  75% of sequence in fragments shorter than    2435 bp
  80% of sequence in fragments shorter than    2725 bp
  85% of sequence in fragments shorter than    3102 bp
  90% of sequence in fragments shorter than    3645 bp
  95% of sequence in fragments shorter than    4641 bp

# BfiI.HhaI (ACTGGG+GCGC) double digest, 3462 thousand fragments (1706594+1756502 sites), 860 bp average fragment length
   5% of sequence in fragments shorter than     285 bp
  10% of sequence in fragments shorter than     460 bp
  15% of sequence in fragments shorter than     633 bp
  20% of sequence in fragments shorter than     786 bp
  25% of sequence in fragments shorter than     943 bp
  30% of sequence in fragments shorter than    1108 bp
  35% of sequence in fragments shorter than    1280 bp
  40% of sequence in fragments shorter than    1460 bp
  45% of sequence in fragments shorter than    1654 bp
  50% of sequence in fragments shorter than    1856 bp
  55% of sequence in fragments shorter than    2079 bp
  60% of sequence in fragments shorter than    2322 bp
  65% of sequence in fragments shorter than    2602 bp
  70% of sequence in fragments shorter than    2893 bp
  75% of sequence in fragments shorter than    3265 bp
  80% of sequence in fragments shorter than    3712 bp
  85% of sequence in fragments shorter than    4297 bp
  90% of sequence in fragments shorter than    5098 bp
  95% of sequence in fragments shorter than    6479 bp

# PpuMI.SspI (RGGWCCY+AATATT) double digest, 3461 thousand fragments (1085138+2377267 sites), 860 bp average fragment length
   5% of sequence in fragments shorter than     298 bp
  10% of sequence in fragments shorter than     455 bp
  15% of sequence in fragments shorter than     592 bp
  20% of sequence in fragments shorter than     723 bp
  25% of sequence in fragments shorter than     852 bp
  30% of sequence in fragments shorter than     979 bp
  35% of sequence in fragments shorter than    1110 bp
  40% of sequence in fragments shorter than    1247 bp
  45% of sequence in fragments shorter than    1386 bp
  50% of sequence in fragments shorter than    1538 bp
  55% of sequence in fragments shorter than    1703 bp
  60% of sequence in fragments shorter than    1880 bp
  65% of sequence in fragments shorter than    2070 bp
  70% of sequence in fragments shorter than    2295 bp
  75% of sequence in fragments shorter than    2544 bp
  80% of sequence in fragments shorter than    2861 bp
  85% of sequence in fragments shorter than    3278 bp
  90% of sequence in fragments shorter than    3881 bp
  95% of sequence in fragments shorter than    5037 bp

# FnuDII.SmlI (CGCG+CTYRAG) double digest, 3461 thousand fragments (733940+2727867 sites), 860 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     466 bp
  15% of sequence in fragments shorter than     613 bp
  20% of sequence in fragments shorter than     752 bp
  25% of sequence in fragments shorter than     890 bp
  30% of sequence in fragments shorter than    1025 bp
  35% of sequence in fragments shorter than    1165 bp
  40% of sequence in fragments shorter than    1312 bp
  45% of sequence in fragments shorter than    1462 bp
  50% of sequence in fragments shorter than    1627 bp
  55% of sequence in fragments shorter than    1805 bp
  60% of sequence in fragments shorter than    1998 bp
  65% of sequence in fragments shorter than    2205 bp
  70% of sequence in fragments shorter than    2448 bp
  75% of sequence in fragments shorter than    2706 bp
  80% of sequence in fragments shorter than    3045 bp
  85% of sequence in fragments shorter than    3485 bp
  90% of sequence in fragments shorter than    4115 bp
  95% of sequence in fragments shorter than    5297 bp

# PvuII.SspI (CAGCTG+AATATT) double digest, 3461 thousand fragments (1084260+2377267 sites), 861 bp average fragment length
   5% of sequence in fragments shorter than     296 bp
  10% of sequence in fragments shorter than     453 bp
  15% of sequence in fragments shorter than     592 bp
  20% of sequence in fragments shorter than     721 bp
  25% of sequence in fragments shorter than     850 bp
  30% of sequence in fragments shorter than     975 bp
  35% of sequence in fragments shorter than    1105 bp
  40% of sequence in fragments shorter than    1242 bp
  45% of sequence in fragments shorter than    1382 bp
  50% of sequence in fragments shorter than    1530 bp
  55% of sequence in fragments shorter than    1696 bp
  60% of sequence in fragments shorter than    1875 bp
  65% of sequence in fragments shorter than    2075 bp
  70% of sequence in fragments shorter than    2304 bp
  75% of sequence in fragments shorter than    2576 bp
  80% of sequence in fragments shorter than    2901 bp
  85% of sequence in fragments shorter than    3326 bp
  90% of sequence in fragments shorter than    3955 bp
  95% of sequence in fragments shorter than    5024 bp

# AvaII.BsaAI (GGWCC+YACGTR) double digest, 3461 thousand fragments (2747690+713801 sites), 861 bp average fragment length
   5% of sequence in fragments shorter than     285 bp
  10% of sequence in fragments shorter than     441 bp
  15% of sequence in fragments shorter than     594 bp
  20% of sequence in fragments shorter than     740 bp
  25% of sequence in fragments shorter than     888 bp
  30% of sequence in fragments shorter than    1024 bp
  35% of sequence in fragments shorter than    1175 bp
  40% of sequence in fragments shorter than    1328 bp
  45% of sequence in fragments shorter than    1496 bp
  50% of sequence in fragments shorter than    1681 bp
  55% of sequence in fragments shorter than    1876 bp
  60% of sequence in fragments shorter than    2094 bp
  65% of sequence in fragments shorter than    2335 bp
  70% of sequence in fragments shorter than    2611 bp
  75% of sequence in fragments shorter than    2930 bp
  80% of sequence in fragments shorter than    3324 bp
  85% of sequence in fragments shorter than    3829 bp
  90% of sequence in fragments shorter than    4550 bp
  95% of sequence in fragments shorter than    5826 bp

# AvaII.BceAI (GGWCC+ACGGC) double digest, 3452 thousand fragments (2747690+705178 sites), 863 bp average fragment length
   5% of sequence in fragments shorter than     274 bp
  10% of sequence in fragments shorter than     438 bp
  15% of sequence in fragments shorter than     600 bp
  20% of sequence in fragments shorter than     757 bp
  25% of sequence in fragments shorter than     914 bp
  30% of sequence in fragments shorter than    1070 bp
  35% of sequence in fragments shorter than    1245 bp
  40% of sequence in fragments shorter than    1424 bp
  45% of sequence in fragments shorter than    1618 bp
  50% of sequence in fragments shorter than    1819 bp
  55% of sequence in fragments shorter than    2040 bp
  60% of sequence in fragments shorter than    2286 bp
  65% of sequence in fragments shorter than    2564 bp
  70% of sequence in fragments shorter than    2871 bp
  75% of sequence in fragments shorter than    3241 bp
  80% of sequence in fragments shorter than    3693 bp
  85% of sequence in fragments shorter than    4282 bp
  90% of sequence in fragments shorter than    5130 bp
  95% of sequence in fragments shorter than    6648 bp

# BstEII.StyI (GGTNACC+CCWWGG) double digest, 3449 thousand fragments (335865+3114107 sites), 863 bp average fragment length
   5% of sequence in fragments shorter than     294 bp
  10% of sequence in fragments shorter than     450 bp
  15% of sequence in fragments shorter than     588 bp
  20% of sequence in fragments shorter than     720 bp
  25% of sequence in fragments shorter than     844 bp
  30% of sequence in fragments shorter than     979 bp
  35% of sequence in fragments shorter than    1117 bp
  40% of sequence in fragments shorter than    1262 bp
  45% of sequence in fragments shorter than    1410 bp
  50% of sequence in fragments shorter than    1574 bp
  55% of sequence in fragments shorter than    1752 bp
  60% of sequence in fragments shorter than    1947 bp
  65% of sequence in fragments shorter than    2168 bp
  70% of sequence in fragments shorter than    2419 bp
  75% of sequence in fragments shorter than    2702 bp
  80% of sequence in fragments shorter than    3041 bp
  85% of sequence in fragments shorter than    3500 bp
  90% of sequence in fragments shorter than    4190 bp
  95% of sequence in fragments shorter than    5549 bp

# AvaII.BciVI (GGWCC+GTATCC) double digest, 3443 thousand fragments (2747690+696093 sites), 865 bp average fragment length
   5% of sequence in fragments shorter than     286 bp
  10% of sequence in fragments shorter than     446 bp
  15% of sequence in fragments shorter than     598 bp
  20% of sequence in fragments shorter than     740 bp
  25% of sequence in fragments shorter than     879 bp
  30% of sequence in fragments shorter than    1022 bp
  35% of sequence in fragments shorter than    1175 bp
  40% of sequence in fragments shorter than    1335 bp
  45% of sequence in fragments shorter than    1502 bp
  50% of sequence in fragments shorter than    1682 bp
  55% of sequence in fragments shorter than    1865 bp
  60% of sequence in fragments shorter than    2074 bp
  65% of sequence in fragments shorter than    2309 bp
  70% of sequence in fragments shorter than    2579 bp
  75% of sequence in fragments shorter than    2887 bp
  80% of sequence in fragments shorter than    3269 bp
  85% of sequence in fragments shorter than    3753 bp
  90% of sequence in fragments shorter than    4448 bp
  95% of sequence in fragments shorter than    5703 bp

# BsaAI.SmlI (YACGTR+CTYRAG) double digest, 3441 thousand fragments (713801+2727867 sites), 865 bp average fragment length
   5% of sequence in fragments shorter than     300 bp
  10% of sequence in fragments shorter than     457 bp
  15% of sequence in fragments shorter than     592 bp
  20% of sequence in fragments shorter than     712 bp
  25% of sequence in fragments shorter than     837 bp
  30% of sequence in fragments shorter than     961 bp
  35% of sequence in fragments shorter than    1083 bp
  40% of sequence in fragments shorter than    1212 bp
  45% of sequence in fragments shorter than    1345 bp
  50% of sequence in fragments shorter than    1481 bp
  55% of sequence in fragments shorter than    1637 bp
  60% of sequence in fragments shorter than    1804 bp
  65% of sequence in fragments shorter than    1987 bp
  70% of sequence in fragments shorter than    2184 bp
  75% of sequence in fragments shorter than    2428 bp
  80% of sequence in fragments shorter than    2709 bp
  85% of sequence in fragments shorter than    3069 bp
  90% of sequence in fragments shorter than    3586 bp
  95% of sequence in fragments shorter than    4497 bp

# BstEII.NspI (GGTNACC+RCATGY) double digest, 3439 thousand fragments (335865+3104359 sites), 866 bp average fragment length
   5% of sequence in fragments shorter than     301 bp
  10% of sequence in fragments shorter than     461 bp
  15% of sequence in fragments shorter than     604 bp
  20% of sequence in fragments shorter than     736 bp
  25% of sequence in fragments shorter than     866 bp
  30% of sequence in fragments shorter than     995 bp
  35% of sequence in fragments shorter than    1125 bp
  40% of sequence in fragments shorter than    1259 bp
  45% of sequence in fragments shorter than    1402 bp
  50% of sequence in fragments shorter than    1552 bp
  55% of sequence in fragments shorter than    1712 bp
  60% of sequence in fragments shorter than    1886 bp
  65% of sequence in fragments shorter than    2080 bp
  70% of sequence in fragments shorter than    2295 bp
  75% of sequence in fragments shorter than    2554 bp
  80% of sequence in fragments shorter than    2860 bp
  85% of sequence in fragments shorter than    3255 bp
  90% of sequence in fragments shorter than    3788 bp
  95% of sequence in fragments shorter than    4763 bp

# BceAI.SmlI (ACGGC+CTYRAG) double digest, 3432 thousand fragments (705178+2727867 sites), 868 bp average fragment length
   5% of sequence in fragments shorter than     294 bp
  10% of sequence in fragments shorter than     453 bp
  15% of sequence in fragments shorter than     591 bp
  20% of sequence in fragments shorter than     718 bp
  25% of sequence in fragments shorter than     846 bp
  30% of sequence in fragments shorter than     974 bp
  35% of sequence in fragments shorter than    1102 bp
  40% of sequence in fragments shorter than    1237 bp
  45% of sequence in fragments shorter than    1379 bp
  50% of sequence in fragments shorter than    1530 bp
  55% of sequence in fragments shorter than    1695 bp
  60% of sequence in fragments shorter than    1875 bp
  65% of sequence in fragments shorter than    2069 bp
  70% of sequence in fragments shorter than    2293 bp
  75% of sequence in fragments shorter than    2558 bp
  80% of sequence in fragments shorter than    2847 bp
  85% of sequence in fragments shorter than    3251 bp
  90% of sequence in fragments shorter than    3827 bp
  95% of sequence in fragments shorter than    4905 bp

# BciVI.SmlI (GTATCC+CTYRAG) double digest, 3423 thousand fragments (696093+2727867 sites), 870 bp average fragment length
   5% of sequence in fragments shorter than     301 bp
  10% of sequence in fragments shorter than     460 bp
  15% of sequence in fragments shorter than     596 bp
  20% of sequence in fragments shorter than     720 bp
  25% of sequence in fragments shorter than     844 bp
  30% of sequence in fragments shorter than     966 bp
  35% of sequence in fragments shorter than    1090 bp
  40% of sequence in fragments shorter than    1219 bp
  45% of sequence in fragments shorter than    1355 bp
  50% of sequence in fragments shorter than    1498 bp
  55% of sequence in fragments shorter than    1655 bp
  60% of sequence in fragments shorter than    1818 bp
  65% of sequence in fragments shorter than    2003 bp
  70% of sequence in fragments shorter than    2206 bp
  75% of sequence in fragments shorter than    2450 bp
  80% of sequence in fragments shorter than    2731 bp
  85% of sequence in fragments shorter than    3102 bp
  90% of sequence in fragments shorter than    3634 bp
  95% of sequence in fragments shorter than    4582 bp

# AccI.HpaII (GTMKAC+CCGG) double digest, 3418 thousand fragments (1101063+2317722 sites), 871 bp average fragment length
   5% of sequence in fragments shorter than     282 bp
  10% of sequence in fragments shorter than     476 bp
  15% of sequence in fragments shorter than     649 bp
  20% of sequence in fragments shorter than     813 bp
  25% of sequence in fragments shorter than     979 bp
  30% of sequence in fragments shorter than    1145 bp
  35% of sequence in fragments shorter than    1313 bp
  40% of sequence in fragments shorter than    1488 bp
  45% of sequence in fragments shorter than    1676 bp
  50% of sequence in fragments shorter than    1874 bp
  55% of sequence in fragments shorter than    2086 bp
  60% of sequence in fragments shorter than    2327 bp
  65% of sequence in fragments shorter than    2586 bp
  70% of sequence in fragments shorter than    2883 bp
  75% of sequence in fragments shorter than    3239 bp
  80% of sequence in fragments shorter than    3668 bp
  85% of sequence in fragments shorter than    4203 bp
  90% of sequence in fragments shorter than    4992 bp
  95% of sequence in fragments shorter than    6364 bp

# SexAI.StyI (ACCWGGT+CCWWGG) double digest, 3411 thousand fragments (297900+3114107 sites), 873 bp average fragment length
   5% of sequence in fragments shorter than     294 bp
  10% of sequence in fragments shorter than     453 bp
  15% of sequence in fragments shorter than     591 bp
  20% of sequence in fragments shorter than     723 bp
  25% of sequence in fragments shorter than     848 bp
  30% of sequence in fragments shorter than     982 bp
  35% of sequence in fragments shorter than    1120 bp
  40% of sequence in fragments shorter than    1264 bp
  45% of sequence in fragments shorter than    1412 bp
  50% of sequence in fragments shorter than    1577 bp
  55% of sequence in fragments shorter than    1756 bp
  60% of sequence in fragments shorter than    1953 bp
  65% of sequence in fragments shorter than    2176 bp
  70% of sequence in fragments shorter than    2429 bp
  75% of sequence in fragments shorter than    2729 bp
  80% of sequence in fragments shorter than    3088 bp
  85% of sequence in fragments shorter than    3521 bp
  90% of sequence in fragments shorter than    4210 bp
  95% of sequence in fragments shorter than    5569 bp

# HhaI.PsiI (GCGC+TTATAA) double digest, 3403 thousand fragments (1756502+1647912 sites), 875 bp average fragment length
   5% of sequence in fragments shorter than     304 bp
  10% of sequence in fragments shorter than     485 bp
  15% of sequence in fragments shorter than     649 bp
  20% of sequence in fragments shorter than     791 bp
  25% of sequence in fragments shorter than     940 bp
  30% of sequence in fragments shorter than    1088 bp
  35% of sequence in fragments shorter than    1243 bp
  40% of sequence in fragments shorter than    1402 bp
  45% of sequence in fragments shorter than    1571 bp
  50% of sequence in fragments shorter than    1747 bp
  55% of sequence in fragments shorter than    1933 bp
  60% of sequence in fragments shorter than    2140 bp
  65% of sequence in fragments shorter than    2370 bp
  70% of sequence in fragments shorter than    2630 bp
  75% of sequence in fragments shorter than    2928 bp
  80% of sequence in fragments shorter than    3294 bp
  85% of sequence in fragments shorter than    3742 bp
  90% of sequence in fragments shorter than    4387 bp
  95% of sequence in fragments shorter than    5485 bp

# HpaII.PpuMI (CCGG+RGGWCCY) double digest, 3402 thousand fragments (2317722+1085138 sites), 875 bp average fragment length
   5% of sequence in fragments shorter than     263 bp
  10% of sequence in fragments shorter than     467 bp
  15% of sequence in fragments shorter than     662 bp
  20% of sequence in fragments shorter than     854 bp
  25% of sequence in fragments shorter than    1050 bp
  30% of sequence in fragments shorter than    1254 bp
  35% of sequence in fragments shorter than    1473 bp
  40% of sequence in fragments shorter than    1700 bp
  45% of sequence in fragments shorter than    1947 bp
  50% of sequence in fragments shorter than    2216 bp
  55% of sequence in fragments shorter than    2509 bp
  60% of sequence in fragments shorter than    2841 bp
  65% of sequence in fragments shorter than    3217 bp
  70% of sequence in fragments shorter than    3644 bp
  75% of sequence in fragments shorter than    4162 bp
  80% of sequence in fragments shorter than    4814 bp
  85% of sequence in fragments shorter than    5679 bp
  90% of sequence in fragments shorter than    6953 bp
  95% of sequence in fragments shorter than    9492 bp

# NspI.SexAI (RCATGY+ACCWGGT) double digest, 3401 thousand fragments (3104359+297900 sites), 876 bp average fragment length
   5% of sequence in fragments shorter than     306 bp
  10% of sequence in fragments shorter than     467 bp
  15% of sequence in fragments shorter than     612 bp
  20% of sequence in fragments shorter than     745 bp
  25% of sequence in fragments shorter than     876 bp
  30% of sequence in fragments shorter than    1006 bp
  35% of sequence in fragments shorter than    1137 bp
  40% of sequence in fragments shorter than    1272 bp
  45% of sequence in fragments shorter than    1416 bp
  50% of sequence in fragments shorter than    1568 bp
  55% of sequence in fragments shorter than    1730 bp
  60% of sequence in fragments shorter than    1906 bp
  65% of sequence in fragments shorter than    2098 bp
  70% of sequence in fragments shorter than    2321 bp
  75% of sequence in fragments shorter than    2585 bp
  80% of sequence in fragments shorter than    2899 bp
  85% of sequence in fragments shorter than    3304 bp
  90% of sequence in fragments shorter than    3861 bp
  95% of sequence in fragments shorter than    4980 bp

# KpnI.StyI (GGTACC+CCWWGG) double digest, 3401 thousand fragments (287974+3114107 sites), 876 bp average fragment length
   5% of sequence in fragments shorter than     292 bp
  10% of sequence in fragments shorter than     454 bp
  15% of sequence in fragments shorter than     598 bp
  20% of sequence in fragments shorter than     731 bp
  25% of sequence in fragments shorter than     852 bp
  30% of sequence in fragments shorter than     986 bp
  35% of sequence in fragments shorter than    1122 bp
  40% of sequence in fragments shorter than    1267 bp
  45% of sequence in fragments shorter than    1417 bp
  50% of sequence in fragments shorter than    1580 bp
  55% of sequence in fragments shorter than    1754 bp
  60% of sequence in fragments shorter than    1947 bp
  65% of sequence in fragments shorter than    2169 bp
  70% of sequence in fragments shorter than    2414 bp
  75% of sequence in fragments shorter than    2709 bp
  80% of sequence in fragments shorter than    3072 bp
  85% of sequence in fragments shorter than    3545 bp
  90% of sequence in fragments shorter than    4243 bp
  95% of sequence in fragments shorter than    5628 bp

# HpaII.PvuII (CCGG+CAGCTG) double digest, 3401 thousand fragments (2317722+1084260 sites), 876 bp average fragment length
   5% of sequence in fragments shorter than     263 bp
  10% of sequence in fragments shorter than     466 bp
  15% of sequence in fragments shorter than     654 bp
  20% of sequence in fragments shorter than     840 bp
  25% of sequence in fragments shorter than    1029 bp
  30% of sequence in fragments shorter than    1225 bp
  35% of sequence in fragments shorter than    1432 bp
  40% of sequence in fragments shorter than    1655 bp
  45% of sequence in fragments shorter than    1896 bp
  50% of sequence in fragments shorter than    2157 bp
  55% of sequence in fragments shorter than    2446 bp
  60% of sequence in fragments shorter than    2768 bp
  65% of sequence in fragments shorter than    3129 bp
  70% of sequence in fragments shorter than    3555 bp
  75% of sequence in fragments shorter than    4063 bp
  80% of sequence in fragments shorter than    4690 bp
  85% of sequence in fragments shorter than    5509 bp
  90% of sequence in fragments shorter than    6712 bp
  95% of sequence in fragments shorter than    9029 bp

# NheI.StyI (GCTAGC+CCWWGG) double digest, 3401 thousand fragments (287384+3114107 sites), 876 bp average fragment length
   5% of sequence in fragments shorter than     297 bp
  10% of sequence in fragments shorter than     455 bp
  15% of sequence in fragments shorter than     598 bp
  20% of sequence in fragments shorter than     733 bp
  25% of sequence in fragments shorter than     858 bp
  30% of sequence in fragments shorter than     991 bp
  35% of sequence in fragments shorter than    1128 bp
  40% of sequence in fragments shorter than    1268 bp
  45% of sequence in fragments shorter than    1411 bp
  50% of sequence in fragments shorter than    1572 bp
  55% of sequence in fragments shorter than    1748 bp
  60% of sequence in fragments shorter than    1931 bp
  65% of sequence in fragments shorter than    2145 bp
  70% of sequence in fragments shorter than    2392 bp
  75% of sequence in fragments shorter than    2682 bp
  80% of sequence in fragments shorter than    3037 bp
  85% of sequence in fragments shorter than    3496 bp
  90% of sequence in fragments shorter than    4169 bp
  95% of sequence in fragments shorter than    5477 bp

# KpnI.NspI (GGTACC+RCATGY) double digest, 3391 thousand fragments (287974+3104359 sites), 878 bp average fragment length
   5% of sequence in fragments shorter than     308 bp
  10% of sequence in fragments shorter than     472 bp
  15% of sequence in fragments shorter than     618 bp
  20% of sequence in fragments shorter than     752 bp
  25% of sequence in fragments shorter than     884 bp
  30% of sequence in fragments shorter than    1006 bp
  35% of sequence in fragments shorter than    1138 bp
  40% of sequence in fragments shorter than    1265 bp
  45% of sequence in fragments shorter than    1404 bp
  50% of sequence in fragments shorter than    1554 bp
  55% of sequence in fragments shorter than    1708 bp
  60% of sequence in fragments shorter than    1881 bp
  65% of sequence in fragments shorter than    2073 bp
  70% of sequence in fragments shorter than    2282 bp
  75% of sequence in fragments shorter than    2537 bp
  80% of sequence in fragments shorter than    2838 bp
  85% of sequence in fragments shorter than    3231 bp
  90% of sequence in fragments shorter than    3787 bp
  95% of sequence in fragments shorter than    4845 bp

# NheI.NspI (GCTAGC+RCATGY) double digest, 3391 thousand fragments (287384+3104359 sites), 878 bp average fragment length
   5% of sequence in fragments shorter than     309 bp
  10% of sequence in fragments shorter than     474 bp
  15% of sequence in fragments shorter than     621 bp
  20% of sequence in fragments shorter than     754 bp
  25% of sequence in fragments shorter than     876 bp
  30% of sequence in fragments shorter than    1005 bp
  35% of sequence in fragments shorter than    1135 bp
  40% of sequence in fragments shorter than    1270 bp
  45% of sequence in fragments shorter than    1414 bp
  50% of sequence in fragments shorter than    1566 bp
  55% of sequence in fragments shorter than    1719 bp
  60% of sequence in fragments shorter than    1886 bp
  65% of sequence in fragments shorter than    2077 bp
  70% of sequence in fragments shorter than    2292 bp
  75% of sequence in fragments shorter than    2546 bp
  80% of sequence in fragments shorter than    2842 bp
  85% of sequence in fragments shorter than    3235 bp
  90% of sequence in fragments shorter than    3794 bp
  95% of sequence in fragments shorter than    4848 bp

# AflII.AvaII (CTTAAG+GGWCC) double digest, 3390 thousand fragments (643284+2747690 sites), 878 bp average fragment length
   5% of sequence in fragments shorter than     290 bp
  10% of sequence in fragments shorter than     452 bp
  15% of sequence in fragments shorter than     605 bp
  20% of sequence in fragments shorter than     748 bp
  25% of sequence in fragments shorter than     890 bp
  30% of sequence in fragments shorter than    1034 bp
  35% of sequence in fragments shorter than    1190 bp
  40% of sequence in fragments shorter than    1351 bp
  45% of sequence in fragments shorter than    1520 bp
  50% of sequence in fragments shorter than    1702 bp
  55% of sequence in fragments shorter than    1890 bp
  60% of sequence in fragments shorter than    2104 bp
  65% of sequence in fragments shorter than    2340 bp
  70% of sequence in fragments shorter than    2611 bp
  75% of sequence in fragments shorter than    2919 bp
  80% of sequence in fragments shorter than    3304 bp
  85% of sequence in fragments shorter than    3800 bp
  90% of sequence in fragments shorter than    4518 bp
  95% of sequence in fragments shorter than    5859 bp

# AvaI.SspI (CYCGRG+AATATT) double digest, 3382 thousand fragments (1005714+2377267 sites), 880 bp average fragment length
   5% of sequence in fragments shorter than     302 bp
  10% of sequence in fragments shorter than     461 bp
  15% of sequence in fragments shorter than     602 bp
  20% of sequence in fragments shorter than     736 bp
  25% of sequence in fragments shorter than     870 bp
  30% of sequence in fragments shorter than    1006 bp
  35% of sequence in fragments shorter than    1145 bp
  40% of sequence in fragments shorter than    1290 bp
  45% of sequence in fragments shorter than    1439 bp
  50% of sequence in fragments shorter than    1599 bp
  55% of sequence in fragments shorter than    1774 bp
  60% of sequence in fragments shorter than    1967 bp
  65% of sequence in fragments shorter than    2182 bp
  70% of sequence in fragments shorter than    2427 bp
  75% of sequence in fragments shorter than    2722 bp
  80% of sequence in fragments shorter than    3080 bp
  85% of sequence in fragments shorter than    3555 bp
  90% of sequence in fragments shorter than    4220 bp
  95% of sequence in fragments shorter than    5416 bp

# AvaII.HaeII (GGWCC+RGCGCY) double digest, 3377 thousand fragments (2747690+630101 sites), 882 bp average fragment length
   5% of sequence in fragments shorter than     280 bp
  10% of sequence in fragments shorter than     445 bp
  15% of sequence in fragments shorter than     610 bp
  20% of sequence in fragments shorter than     765 bp
  25% of sequence in fragments shorter than     924 bp
  30% of sequence in fragments shorter than    1083 bp
  35% of sequence in fragments shorter than    1262 bp
  40% of sequence in fragments shorter than    1449 bp
  45% of sequence in fragments shorter than    1646 bp
  50% of sequence in fragments shorter than    1853 bp
  55% of sequence in fragments shorter than    2082 bp
  60% of sequence in fragments shorter than    2334 bp
  65% of sequence in fragments shorter than    2614 bp
  70% of sequence in fragments shorter than    2932 bp
  75% of sequence in fragments shorter than    3308 bp
  80% of sequence in fragments shorter than    3763 bp
  85% of sequence in fragments shorter than    4349 bp
  90% of sequence in fragments shorter than    5176 bp
  95% of sequence in fragments shorter than    6584 bp

# EsaBC3I.HhaI (TCGA+GCGC) double digest, 3359 thousand fragments (1603346+1756502 sites), 887 bp average fragment length
   5% of sequence in fragments shorter than     294 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     681 bp
  20% of sequence in fragments shorter than     856 bp
  25% of sequence in fragments shorter than    1035 bp
  30% of sequence in fragments shorter than    1224 bp
  35% of sequence in fragments shorter than    1420 bp
  40% of sequence in fragments shorter than    1629 bp
  45% of sequence in fragments shorter than    1851 bp
  50% of sequence in fragments shorter than    2092 bp
  55% of sequence in fragments shorter than    2354 bp
  60% of sequence in fragments shorter than    2645 bp
  65% of sequence in fragments shorter than    2962 bp
  70% of sequence in fragments shorter than    3331 bp
  75% of sequence in fragments shorter than    3766 bp
  80% of sequence in fragments shorter than    4292 bp
  85% of sequence in fragments shorter than    4968 bp
  90% of sequence in fragments shorter than    5914 bp
  95% of sequence in fragments shorter than    7529 bp

# HaeII.SmlI (RGCGCY+CTYRAG) double digest, 3357 thousand fragments (630101+2727867 sites), 887 bp average fragment length
   5% of sequence in fragments shorter than     302 bp
  10% of sequence in fragments shorter than     456 bp
  15% of sequence in fragments shorter than     600 bp
  20% of sequence in fragments shorter than     727 bp
  25% of sequence in fragments shorter than     861 bp
  30% of sequence in fragments shorter than     993 bp
  35% of sequence in fragments shorter than    1125 bp
  40% of sequence in fragments shorter than    1266 bp
  45% of sequence in fragments shorter than    1412 bp
  50% of sequence in fragments shorter than    1569 bp
  55% of sequence in fragments shorter than    1740 bp
  60% of sequence in fragments shorter than    1925 bp
  65% of sequence in fragments shorter than    2121 bp
  70% of sequence in fragments shorter than    2349 bp
  75% of sequence in fragments shorter than    2616 bp
  80% of sequence in fragments shorter than    2914 bp
  85% of sequence in fragments shorter than    3319 bp
  90% of sequence in fragments shorter than    3877 bp
  95% of sequence in fragments shorter than    4837 bp

# BspHI.SspI (TCATGA+AATATT) double digest, 3355 thousand fragments (978290+2377267 sites), 888 bp average fragment length
   5% of sequence in fragments shorter than     297 bp
  10% of sequence in fragments shorter than     460 bp
  15% of sequence in fragments shorter than     603 bp
  20% of sequence in fragments shorter than     741 bp
  25% of sequence in fragments shorter than     882 bp
  30% of sequence in fragments shorter than    1026 bp
  35% of sequence in fragments shorter than    1174 bp
  40% of sequence in fragments shorter than    1330 bp
  45% of sequence in fragments shorter than    1492 bp
  50% of sequence in fragments shorter than    1672 bp
  55% of sequence in fragments shorter than    1864 bp
  60% of sequence in fragments shorter than    2076 bp
  65% of sequence in fragments shorter than    2310 bp
  70% of sequence in fragments shorter than    2592 bp
  75% of sequence in fragments shorter than    2942 bp
  80% of sequence in fragments shorter than    3389 bp
  85% of sequence in fragments shorter than    4015 bp
  90% of sequence in fragments shorter than    5009 bp
  95% of sequence in fragments shorter than    7194 bp

# BfiI.PsiI (ACTGGG+TTATAA) double digest, 3354 thousand fragments (1706594+1647912 sites), 888 bp average fragment length
   5% of sequence in fragments shorter than     309 bp
  10% of sequence in fragments shorter than     471 bp
  15% of sequence in fragments shorter than     615 bp
  20% of sequence in fragments shorter than     747 bp
  25% of sequence in fragments shorter than     878 bp
  30% of sequence in fragments shorter than    1007 bp
  35% of sequence in fragments shorter than    1138 bp
  40% of sequence in fragments shorter than    1275 bp
  45% of sequence in fragments shorter than    1417 bp
  50% of sequence in fragments shorter than    1569 bp
  55% of sequence in fragments shorter than    1729 bp
  60% of sequence in fragments shorter than    1907 bp
  65% of sequence in fragments shorter than    2091 bp
  70% of sequence in fragments shorter than    2311 bp
  75% of sequence in fragments shorter than    2570 bp
  80% of sequence in fragments shorter than    2850 bp
  85% of sequence in fragments shorter than    3235 bp
  90% of sequence in fragments shorter than    3815 bp
  95% of sequence in fragments shorter than    4911 bp

# AvaII.SacI (GGWCC+GAGCTC) double digest, 3350 thousand fragments (2747690+603323 sites), 889 bp average fragment length
   5% of sequence in fragments shorter than     287 bp
  10% of sequence in fragments shorter than     453 bp
  15% of sequence in fragments shorter than     614 bp
  20% of sequence in fragments shorter than     765 bp
  25% of sequence in fragments shorter than     920 bp
  30% of sequence in fragments shorter than    1070 bp
  35% of sequence in fragments shorter than    1238 bp
  40% of sequence in fragments shorter than    1415 bp
  45% of sequence in fragments shorter than    1599 bp
  50% of sequence in fragments shorter than    1804 bp
  55% of sequence in fragments shorter than    2021 bp
  60% of sequence in fragments shorter than    2264 bp
  65% of sequence in fragments shorter than    2537 bp
  70% of sequence in fragments shorter than    2849 bp
  75% of sequence in fragments shorter than    3216 bp
  80% of sequence in fragments shorter than    3664 bp
  85% of sequence in fragments shorter than    4248 bp
  90% of sequence in fragments shorter than    5087 bp
  95% of sequence in fragments shorter than    6600 bp

# BsrBI.StyI (CCGCTC+CCWWGG) double digest, 3349 thousand fragments (235523+3114107 sites), 889 bp average fragment length
   5% of sequence in fragments shorter than     298 bp
  10% of sequence in fragments shorter than     461 bp
  15% of sequence in fragments shorter than     604 bp
  20% of sequence in fragments shorter than     740 bp
  25% of sequence in fragments shorter than     871 bp
  30% of sequence in fragments shorter than    1012 bp
  35% of sequence in fragments shorter than    1157 bp
  40% of sequence in fragments shorter than    1307 bp
  45% of sequence in fragments shorter than    1463 bp
  50% of sequence in fragments shorter than    1628 bp
  55% of sequence in fragments shorter than    1813 bp
  60% of sequence in fragments shorter than    2019 bp
  65% of sequence in fragments shorter than    2251 bp
  70% of sequence in fragments shorter than    2514 bp
  75% of sequence in fragments shorter than    2821 bp
  80% of sequence in fragments shorter than    3190 bp
  85% of sequence in fragments shorter than    3679 bp
  90% of sequence in fragments shorter than    4397 bp
  95% of sequence in fragments shorter than    5789 bp

# KasI.StyI (GGCGCC+CCWWGG) double digest, 3344 thousand fragments (230381+3114107 sites), 891 bp average fragment length
   5% of sequence in fragments shorter than     298 bp
  10% of sequence in fragments shorter than     461 bp
  15% of sequence in fragments shorter than     602 bp
  20% of sequence in fragments shorter than     738 bp
  25% of sequence in fragments shorter than     865 bp
  30% of sequence in fragments shorter than    1004 bp
  35% of sequence in fragments shorter than    1148 bp
  40% of sequence in fragments shorter than    1297 bp
  45% of sequence in fragments shorter than    1452 bp
  50% of sequence in fragments shorter than    1623 bp
  55% of sequence in fragments shorter than    1808 bp
  60% of sequence in fragments shorter than    2014 bp
  65% of sequence in fragments shorter than    2246 bp
  70% of sequence in fragments shorter than    2511 bp
  75% of sequence in fragments shorter than    2815 bp
  80% of sequence in fragments shorter than    3169 bp
  85% of sequence in fragments shorter than    3659 bp
  90% of sequence in fragments shorter than    4372 bp
  95% of sequence in fragments shorter than    5800 bp

# AvaII.AvrII (GGWCC+CCTAGG) double digest, 3342 thousand fragments (2747690+594956 sites), 891 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     464 bp
  15% of sequence in fragments shorter than     618 bp
  20% of sequence in fragments shorter than     771 bp
  25% of sequence in fragments shorter than     915 bp
  30% of sequence in fragments shorter than    1073 bp
  35% of sequence in fragments shorter than    1242 bp
  40% of sequence in fragments shorter than    1414 bp
  45% of sequence in fragments shorter than    1600 bp
  50% of sequence in fragments shorter than    1793 bp
  55% of sequence in fragments shorter than    2005 bp
  60% of sequence in fragments shorter than    2242 bp
  65% of sequence in fragments shorter than    2506 bp
  70% of sequence in fragments shorter than    2800 bp
  75% of sequence in fragments shorter than    3157 bp
  80% of sequence in fragments shorter than    3592 bp
  85% of sequence in fragments shorter than    4161 bp
  90% of sequence in fragments shorter than    4986 bp
  95% of sequence in fragments shorter than    6515 bp

# StyI.SwaI (CCWWGG+ATTTAAAT) double digest, 3340 thousand fragments (3114107+226602 sites), 892 bp average fragment length
   5% of sequence in fragments shorter than     304 bp
  10% of sequence in fragments shorter than     466 bp
  15% of sequence in fragments shorter than     605 bp
  20% of sequence in fragments shorter than     737 bp
  25% of sequence in fragments shorter than     862 bp
  30% of sequence in fragments shorter than     996 bp
  35% of sequence in fragments shorter than    1133 bp
  40% of sequence in fragments shorter than    1275 bp
  45% of sequence in fragments shorter than    1421 bp
  50% of sequence in fragments shorter than    1581 bp
  55% of sequence in fragments shorter than    1754 bp
  60% of sequence in fragments shorter than    1943 bp
  65% of sequence in fragments shorter than    2158 bp
  70% of sequence in fragments shorter than    2400 bp
  75% of sequence in fragments shorter than    2684 bp
  80% of sequence in fragments shorter than    3025 bp
  85% of sequence in fragments shorter than    3435 bp
  90% of sequence in fragments shorter than    4081 bp
  95% of sequence in fragments shorter than    5334 bp

# BsrBI.NspI (CCGCTC+RCATGY) double digest, 3339 thousand fragments (235523+3104359 sites), 892 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     478 bp
  15% of sequence in fragments shorter than     627 bp
  20% of sequence in fragments shorter than     763 bp
  25% of sequence in fragments shorter than     897 bp
  30% of sequence in fragments shorter than    1030 bp
  35% of sequence in fragments shorter than    1164 bp
  40% of sequence in fragments shorter than    1302 bp
  45% of sequence in fragments shorter than    1448 bp
  50% of sequence in fragments shorter than    1603 bp
  55% of sequence in fragments shorter than    1766 bp
  60% of sequence in fragments shorter than    1944 bp
  65% of sequence in fragments shorter than    2139 bp
  70% of sequence in fragments shorter than    2362 bp
  75% of sequence in fragments shorter than    2625 bp
  80% of sequence in fragments shorter than    2926 bp
  85% of sequence in fragments shorter than    3323 bp
  90% of sequence in fragments shorter than    3870 bp
  95% of sequence in fragments shorter than    4909 bp

# KasI.NspI (GGCGCC+RCATGY) double digest, 3334 thousand fragments (230381+3104359 sites), 893 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     475 bp
  15% of sequence in fragments shorter than     622 bp
  20% of sequence in fragments shorter than     757 bp
  25% of sequence in fragments shorter than     889 bp
  30% of sequence in fragments shorter than    1021 bp
  35% of sequence in fragments shorter than    1154 bp
  40% of sequence in fragments shorter than    1291 bp
  45% of sequence in fragments shorter than    1435 bp
  50% of sequence in fragments shorter than    1589 bp
  55% of sequence in fragments shorter than    1750 bp
  60% of sequence in fragments shorter than    1927 bp
  65% of sequence in fragments shorter than    2121 bp
  70% of sequence in fragments shorter than    2344 bp
  75% of sequence in fragments shorter than    2608 bp
  80% of sequence in fragments shorter than    2923 bp
  85% of sequence in fragments shorter than    3329 bp
  90% of sequence in fragments shorter than    3884 bp
  95% of sequence in fragments shorter than    4982 bp

# SacI.SmlI (GAGCTC+CTYRAG) double digest, 3330 thousand fragments (603323+2727867 sites), 894 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     476 bp
  15% of sequence in fragments shorter than     618 bp
  20% of sequence in fragments shorter than     751 bp
  25% of sequence in fragments shorter than     883 bp
  30% of sequence in fragments shorter than    1013 bp
  35% of sequence in fragments shorter than    1148 bp
  40% of sequence in fragments shorter than    1288 bp
  45% of sequence in fragments shorter than    1431 bp
  50% of sequence in fragments shorter than    1587 bp
  55% of sequence in fragments shorter than    1754 bp
  60% of sequence in fragments shorter than    1936 bp
  65% of sequence in fragments shorter than    2129 bp
  70% of sequence in fragments shorter than    2337 bp
  75% of sequence in fragments shorter than    2596 bp
  80% of sequence in fragments shorter than    2909 bp
  85% of sequence in fragments shorter than    3318 bp
  90% of sequence in fragments shorter than    3903 bp
  95% of sequence in fragments shorter than    4950 bp

# NspI.SwaI (RCATGY+ATTTAAAT) double digest, 3330 thousand fragments (3104359+226602 sites), 894 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     477 bp
  15% of sequence in fragments shorter than     626 bp
  20% of sequence in fragments shorter than     761 bp
  25% of sequence in fragments shorter than     896 bp
  30% of sequence in fragments shorter than    1030 bp
  35% of sequence in fragments shorter than    1166 bp
  40% of sequence in fragments shorter than    1306 bp
  45% of sequence in fragments shorter than    1454 bp
  50% of sequence in fragments shorter than    1612 bp
  55% of sequence in fragments shorter than    1778 bp
  60% of sequence in fragments shorter than    1961 bp
  65% of sequence in fragments shorter than    2160 bp
  70% of sequence in fragments shorter than    2391 bp
  75% of sequence in fragments shorter than    2664 bp
  80% of sequence in fragments shorter than    2991 bp
  85% of sequence in fragments shorter than    3414 bp
  90% of sequence in fragments shorter than    3996 bp
  95% of sequence in fragments shorter than    5180 bp

# AvaI.HpaII (CYCGRG+CCGG) double digest, 3322 thousand fragments (1005714+2317722 sites), 896 bp average fragment length
   5% of sequence in fragments shorter than     305 bp
  10% of sequence in fragments shorter than     563 bp
  15% of sequence in fragments shorter than     811 bp
  20% of sequence in fragments shorter than    1061 bp
  25% of sequence in fragments shorter than    1324 bp
  30% of sequence in fragments shorter than    1603 bp
  35% of sequence in fragments shorter than    1900 bp
  40% of sequence in fragments shorter than    2219 bp
  45% of sequence in fragments shorter than    2575 bp
  50% of sequence in fragments shorter than    2970 bp
  55% of sequence in fragments shorter than    3408 bp
  60% of sequence in fragments shorter than    3898 bp
  65% of sequence in fragments shorter than    4455 bp
  70% of sequence in fragments shorter than    5107 bp
  75% of sequence in fragments shorter than    5872 bp
  80% of sequence in fragments shorter than    6843 bp
  85% of sequence in fragments shorter than    8115 bp
  90% of sequence in fragments shorter than   10002 bp
  95% of sequence in fragments shorter than   13711 bp

# AvrII.SmlI (CCTAGG+CTYRAG) double digest, 3322 thousand fragments (594956+2727867 sites), 896 bp average fragment length
   5% of sequence in fragments shorter than     309 bp
  10% of sequence in fragments shorter than     477 bp
  15% of sequence in fragments shorter than     613 bp
  20% of sequence in fragments shorter than     743 bp
  25% of sequence in fragments shorter than     874 bp
  30% of sequence in fragments shorter than    1005 bp
  35% of sequence in fragments shorter than    1137 bp
  40% of sequence in fragments shorter than    1275 bp
  45% of sequence in fragments shorter than    1418 bp
  50% of sequence in fragments shorter than    1571 bp
  55% of sequence in fragments shorter than    1736 bp
  60% of sequence in fragments shorter than    1917 bp
  65% of sequence in fragments shorter than    2110 bp
  70% of sequence in fragments shorter than    2337 bp
  75% of sequence in fragments shorter than    2600 bp
  80% of sequence in fragments shorter than    2886 bp
  85% of sequence in fragments shorter than    3292 bp
  90% of sequence in fragments shorter than    3869 bp
  95% of sequence in fragments shorter than    4958 bp

# AvaII.HgaI (GGWCC+GACGC) double digest, 3319 thousand fragments (2747690+571889 sites), 897 bp average fragment length
   5% of sequence in fragments shorter than     284 bp
  10% of sequence in fragments shorter than     456 bp
  15% of sequence in fragments shorter than     626 bp
  20% of sequence in fragments shorter than     790 bp
  25% of sequence in fragments shorter than     945 bp
  30% of sequence in fragments shorter than    1120 bp
  35% of sequence in fragments shorter than    1303 bp
  40% of sequence in fragments shorter than    1491 bp
  45% of sequence in fragments shorter than    1695 bp
  50% of sequence in fragments shorter than    1904 bp
  55% of sequence in fragments shorter than    2138 bp
  60% of sequence in fragments shorter than    2396 bp
  65% of sequence in fragments shorter than    2680 bp
  70% of sequence in fragments shorter than    3010 bp
  75% of sequence in fragments shorter than    3398 bp
  80% of sequence in fragments shorter than    3873 bp
  85% of sequence in fragments shorter than    4496 bp
  90% of sequence in fragments shorter than    5381 bp
  95% of sequence in fragments shorter than    6971 bp

# AvaII.EciI (GGWCC+GGCGGA) double digest, 3318 thousand fragments (2747690+571273 sites), 897 bp average fragment length
   5% of sequence in fragments shorter than     288 bp
  10% of sequence in fragments shorter than     452 bp
  15% of sequence in fragments shorter than     610 bp
  20% of sequence in fragments shorter than     764 bp
  25% of sequence in fragments shorter than     915 bp
  30% of sequence in fragments shorter than    1067 bp
  35% of sequence in fragments shorter than    1237 bp
  40% of sequence in fragments shorter than    1413 bp
  45% of sequence in fragments shorter than    1602 bp
  50% of sequence in fragments shorter than    1802 bp
  55% of sequence in fragments shorter than    2021 bp
  60% of sequence in fragments shorter than    2265 bp
  65% of sequence in fragments shorter than    2542 bp
  70% of sequence in fragments shorter than    2849 bp
  75% of sequence in fragments shorter than    3223 bp
  80% of sequence in fragments shorter than    3680 bp
  85% of sequence in fragments shorter than    4282 bp
  90% of sequence in fragments shorter than    5162 bp
  95% of sequence in fragments shorter than    6807 bp

# AvaII.MfeI (GGWCC+CAATTG) double digest, 3311 thousand fragments (2747690+564303 sites), 899 bp average fragment length
   5% of sequence in fragments shorter than     299 bp
  10% of sequence in fragments shorter than     467 bp
  15% of sequence in fragments shorter than     624 bp
  20% of sequence in fragments shorter than     771 bp
  25% of sequence in fragments shorter than     911 bp
  30% of sequence in fragments shorter than    1062 bp
  35% of sequence in fragments shorter than    1222 bp
  40% of sequence in fragments shorter than    1385 bp
  45% of sequence in fragments shorter than    1561 bp
  50% of sequence in fragments shorter than    1744 bp
  55% of sequence in fragments shorter than    1939 bp
  60% of sequence in fragments shorter than    2154 bp
  65% of sequence in fragments shorter than    2392 bp
  70% of sequence in fragments shorter than    2660 bp
  75% of sequence in fragments shorter than    2978 bp
  80% of sequence in fragments shorter than    3365 bp
  85% of sequence in fragments shorter than    3856 bp
  90% of sequence in fragments shorter than    4563 bp
  95% of sequence in fragments shorter than    5822 bp

# AvaIII.SspI (ATGCAT+AATATT) double digest, 3310 thousand fragments (933357+2377267 sites), 900 bp average fragment length
   5% of sequence in fragments shorter than     297 bp
  10% of sequence in fragments shorter than     466 bp
  15% of sequence in fragments shorter than     616 bp
  20% of sequence in fragments shorter than     761 bp
  25% of sequence in fragments shorter than     908 bp
  30% of sequence in fragments shorter than    1057 bp
  35% of sequence in fragments shorter than    1215 bp
  40% of sequence in fragments shorter than    1382 bp
  45% of sequence in fragments shorter than    1554 bp
  50% of sequence in fragments shorter than    1748 bp
  55% of sequence in fragments shorter than    1962 bp
  60% of sequence in fragments shorter than    2204 bp
  65% of sequence in fragments shorter than    2476 bp
  70% of sequence in fragments shorter than    2799 bp
  75% of sequence in fragments shorter than    3199 bp
  80% of sequence in fragments shorter than    3706 bp
  85% of sequence in fragments shorter than    4383 bp
  90% of sequence in fragments shorter than    5451 bp
  95% of sequence in fragments shorter than    7891 bp

# BfiI.EsaBC3I (ACTGGG+TCGA) double digest, 3309 thousand fragments (1706594+1603346 sites), 900 bp average fragment length
   5% of sequence in fragments shorter than     313 bp
  10% of sequence in fragments shorter than     469 bp
  15% of sequence in fragments shorter than     610 bp
  20% of sequence in fragments shorter than     744 bp
  25% of sequence in fragments shorter than     872 bp
  30% of sequence in fragments shorter than    1005 bp
  35% of sequence in fragments shorter than    1139 bp
  40% of sequence in fragments shorter than    1279 bp
  45% of sequence in fragments shorter than    1424 bp
  50% of sequence in fragments shorter than    1582 bp
  55% of sequence in fragments shorter than    1750 bp
  60% of sequence in fragments shorter than    1935 bp
  65% of sequence in fragments shorter than    2142 bp
  70% of sequence in fragments shorter than    2371 bp
  75% of sequence in fragments shorter than    2646 bp
  80% of sequence in fragments shorter than    2969 bp
  85% of sequence in fragments shorter than    3394 bp
  90% of sequence in fragments shorter than    3985 bp
  95% of sequence in fragments shorter than    5014 bp

# HgiCI.HhaI (GGYRCC+GCGC) double digest, 3306 thousand fragments (1550877+1756502 sites), 901 bp average fragment length
   5% of sequence in fragments shorter than     314 bp
  10% of sequence in fragments shorter than     529 bp
  15% of sequence in fragments shorter than     720 bp
  20% of sequence in fragments shorter than     923 bp
  25% of sequence in fragments shorter than    1123 bp
  30% of sequence in fragments shorter than    1313 bp
  35% of sequence in fragments shorter than    1533 bp
  40% of sequence in fragments shorter than    1755 bp
  45% of sequence in fragments shorter than    2001 bp
  50% of sequence in fragments shorter than    2271 bp
  55% of sequence in fragments shorter than    2566 bp
  60% of sequence in fragments shorter than    2885 bp
  65% of sequence in fragments shorter than    3253 bp
  70% of sequence in fragments shorter than    3678 bp
  75% of sequence in fragments shorter than    4176 bp
  80% of sequence in fragments shorter than    4788 bp
  85% of sequence in fragments shorter than    5578 bp
  90% of sequence in fragments shorter than    6679 bp
  95% of sequence in fragments shorter than    8614 bp

# BsmI.HhaI (GAATGC+GCGC) double digest, 3305 thousand fragments (1549350+1756502 sites), 901 bp average fragment length
   5% of sequence in fragments shorter than     274 bp
  10% of sequence in fragments shorter than     510 bp
  15% of sequence in fragments shorter than     713 bp
  20% of sequence in fragments shorter than     908 bp
  25% of sequence in fragments shorter than    1092 bp
  30% of sequence in fragments shorter than    1279 bp
  35% of sequence in fragments shorter than    1473 bp
  40% of sequence in fragments shorter than    1675 bp
  45% of sequence in fragments shorter than    1880 bp
  50% of sequence in fragments shorter than    2102 bp
  55% of sequence in fragments shorter than    2343 bp
  60% of sequence in fragments shorter than    2600 bp
  65% of sequence in fragments shorter than    2886 bp
  70% of sequence in fragments shorter than    3212 bp
  75% of sequence in fragments shorter than    3595 bp
  80% of sequence in fragments shorter than    4052 bp
  85% of sequence in fragments shorter than    4637 bp
  90% of sequence in fragments shorter than    5447 bp
  95% of sequence in fragments shorter than    6819 bp

# SspI.StuI (AATATT+AGGCCT) double digest, 3302 thousand fragments (2377267+925729 sites), 902 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     470 bp
  15% of sequence in fragments shorter than     618 bp
  20% of sequence in fragments shorter than     743 bp
  25% of sequence in fragments shorter than     881 bp
  30% of sequence in fragments shorter than    1023 bp
  35% of sequence in fragments shorter than    1166 bp
  40% of sequence in fragments shorter than    1312 bp
  45% of sequence in fragments shorter than    1463 bp
  50% of sequence in fragments shorter than    1632 bp
  55% of sequence in fragments shorter than    1808 bp
  60% of sequence in fragments shorter than    2001 bp
  65% of sequence in fragments shorter than    2221 bp
  70% of sequence in fragments shorter than    2465 bp
  75% of sequence in fragments shorter than    2756 bp
  80% of sequence in fragments shorter than    3105 bp
  85% of sequence in fragments shorter than    3559 bp
  90% of sequence in fragments shorter than    4187 bp
  95% of sequence in fragments shorter than    5258 bp

# HgaI.SmlI (GACGC+CTYRAG) double digest, 3299 thousand fragments (571889+2727867 sites), 903 bp average fragment length
   5% of sequence in fragments shorter than     307 bp
  10% of sequence in fragments shorter than     472 bp
  15% of sequence in fragments shorter than     615 bp
  20% of sequence in fragments shorter than     748 bp
  25% of sequence in fragments shorter than     882 bp
  30% of sequence in fragments shorter than    1013 bp
  35% of sequence in fragments shorter than    1148 bp
  40% of sequence in fragments shorter than    1290 bp
  45% of sequence in fragments shorter than    1431 bp
  50% of sequence in fragments shorter than    1590 bp
  55% of sequence in fragments shorter than    1759 bp
  60% of sequence in fragments shorter than    1943 bp
  65% of sequence in fragments shorter than    2143 bp
  70% of sequence in fragments shorter than    2376 bp
  75% of sequence in fragments shorter than    2642 bp
  80% of sequence in fragments shorter than    2955 bp
  85% of sequence in fragments shorter than    3375 bp
  90% of sequence in fragments shorter than    3969 bp
  95% of sequence in fragments shorter than    5056 bp

# EciI.SmlI (GGCGGA+CTYRAG) double digest, 3298 thousand fragments (571273+2727867 sites), 903 bp average fragment length
   5% of sequence in fragments shorter than     303 bp
  10% of sequence in fragments shorter than     469 bp
  15% of sequence in fragments shorter than     610 bp
  20% of sequence in fragments shorter than     743 bp
  25% of sequence in fragments shorter than     877 bp
  30% of sequence in fragments shorter than    1010 bp
  35% of sequence in fragments shorter than    1145 bp
  40% of sequence in fragments shorter than    1287 bp
  45% of sequence in fragments shorter than    1435 bp
  50% of sequence in fragments shorter than    1596 bp
  55% of sequence in fragments shorter than    1771 bp
  60% of sequence in fragments shorter than    1959 bp
  65% of sequence in fragments shorter than    2163 bp
  70% of sequence in fragments shorter than    2402 bp
  75% of sequence in fragments shorter than    2657 bp
  80% of sequence in fragments shorter than    2986 bp
  85% of sequence in fragments shorter than    3419 bp
  90% of sequence in fragments shorter than    4037 bp
  95% of sequence in fragments shorter than    5198 bp

# AvaII.SphI (GGWCC+GCATGC) double digest, 3298 thousand fragments (2747690+550952 sites), 903 bp average fragment length
   5% of sequence in fragments shorter than     296 bp
  10% of sequence in fragments shorter than     461 bp
  15% of sequence in fragments shorter than     620 bp
  20% of sequence in fragments shorter than     771 bp
  25% of sequence in fragments shorter than     921 bp
  30% of sequence in fragments shorter than    1071 bp
  35% of sequence in fragments shorter than    1237 bp
  40% of sequence in fragments shorter than    1407 bp
  45% of sequence in fragments shorter than    1590 bp
  50% of sequence in fragments shorter than    1783 bp
  55% of sequence in fragments shorter than    1992 bp
  60% of sequence in fragments shorter than    2225 bp
  65% of sequence in fragments shorter than    2485 bp
  70% of sequence in fragments shorter than    2774 bp
  75% of sequence in fragments shorter than    3123 bp
  80% of sequence in fragments shorter than    3548 bp
  85% of sequence in fragments shorter than    4103 bp
  90% of sequence in fragments shorter than    4908 bp
  95% of sequence in fragments shorter than    6388 bp

# BspHI.HpaII (TCATGA+CCGG) double digest, 3295 thousand fragments (978290+2317722 sites), 904 bp average fragment length
   5% of sequence in fragments shorter than     293 bp
  10% of sequence in fragments shorter than     501 bp
  15% of sequence in fragments shorter than     683 bp
  20% of sequence in fragments shorter than     855 bp
  25% of sequence in fragments shorter than    1028 bp
  30% of sequence in fragments shorter than    1204 bp
  35% of sequence in fragments shorter than    1386 bp
  40% of sequence in fragments shorter than    1575 bp
  45% of sequence in fragments shorter than    1771 bp
  50% of sequence in fragments shorter than    1983 bp
  55% of sequence in fragments shorter than    2206 bp
  60% of sequence in fragments shorter than    2453 bp
  65% of sequence in fragments shorter than    2740 bp
  70% of sequence in fragments shorter than    3067 bp
  75% of sequence in fragments shorter than    3444 bp
  80% of sequence in fragments shorter than    3900 bp
  85% of sequence in fragments shorter than    4500 bp
  90% of sequence in fragments shorter than    5379 bp
  95% of sequence in fragments shorter than    7036 bp

# MfeI.SmlI (CAATTG+CTYRAG) double digest, 3291 thousand fragments (564303+2727867 sites), 905 bp average fragment length
   5% of sequence in fragments shorter than     311 bp
  10% of sequence in fragments shorter than     477 bp
  15% of sequence in fragments shorter than     616 bp
  20% of sequence in fragments shorter than     745 bp
  25% of sequence in fragments shorter than     874 bp
  30% of sequence in fragments shorter than    1002 bp
  35% of sequence in fragments shorter than    1130 bp
  40% of sequence in fragments shorter than    1263 bp
  45% of sequence in fragments shorter than    1406 bp
  50% of sequence in fragments shorter than    1556 bp
  55% of sequence in fragments shorter than    1721 bp
  60% of sequence in fragments shorter than    1895 bp
  65% of sequence in fragments shorter than    2080 bp
  70% of sequence in fragments shorter than    2297 bp
  75% of sequence in fragments shorter than    2550 bp
  80% of sequence in fragments shorter than    2825 bp
  85% of sequence in fragments shorter than    3209 bp
  90% of sequence in fragments shorter than    3750 bp
  95% of sequence in fragments shorter than    4728 bp

# AvaII.ScaI (GGWCC+AGTACT) double digest, 3291 thousand fragments (2747690+543793 sites), 905 bp average fragment length
   5% of sequence in fragments shorter than     301 bp
  10% of sequence in fragments shorter than     467 bp
  15% of sequence in fragments shorter than     624 bp
  20% of sequence in fragments shorter than     772 bp
  25% of sequence in fragments shorter than     918 bp
  30% of sequence in fragments shorter than    1062 bp
  35% of sequence in fragments shorter than    1221 bp
  40% of sequence in fragments shorter than    1384 bp
  45% of sequence in fragments shorter than    1559 bp
  50% of sequence in fragments shorter than    1741 bp
  55% of sequence in fragments shorter than    1936 bp
  60% of sequence in fragments shorter than    2152 bp
  65% of sequence in fragments shorter than    2392 bp
  70% of sequence in fragments shorter than    2660 bp
  75% of sequence in fragments shorter than    2979 bp
  80% of sequence in fragments shorter than    3369 bp
  85% of sequence in fragments shorter than    3872 bp
  90% of sequence in fragments shorter than    4600 bp
  95% of sequence in fragments shorter than    5921 bp

# AclI.StyI (AACGTT+CCWWGG) double digest, 3286 thousand fragments (172706+3114107 sites), 906 bp average fragment length
   5% of sequence in fragments shorter than     307 bp
  10% of sequence in fragments shorter than     472 bp
  15% of sequence in fragments shorter than     615 bp
  20% of sequence in fragments shorter than     750 bp
  25% of sequence in fragments shorter than     880 bp
  30% of sequence in fragments shorter than    1019 bp
  35% of sequence in fragments shorter than    1158 bp
  40% of sequence in fragments shorter than    1305 bp
  45% of sequence in fragments shorter than    1457 bp
  50% of sequence in fragments shorter than    1624 bp
  55% of sequence in fragments shorter than    1804 bp
  60% of sequence in fragments shorter than    2004 bp
  65% of sequence in fragments shorter than    2224 bp
  70% of sequence in fragments shorter than    2479 bp
  75% of sequence in fragments shorter than    2771 bp
  80% of sequence in fragments shorter than    3123 bp
  85% of sequence in fragments shorter than    3565 bp
  90% of sequence in fragments shorter than    4236 bp
  95% of sequence in fragments shorter than    5512 bp

# NdeI.SspI (CATATG+AATATT) double digest, 3280 thousand fragments (903360+2377267 sites), 908 bp average fragment length
   5% of sequence in fragments shorter than     295 bp
  10% of sequence in fragments shorter than     462 bp
  15% of sequence in fragments shorter than     615 bp
  20% of sequence in fragments shorter than     761 bp
  25% of sequence in fragments shorter than     905 bp
  30% of sequence in fragments shorter than    1055 bp
  35% of sequence in fragments shorter than    1205 bp
  40% of sequence in fragments shorter than    1372 bp
  45% of sequence in fragments shorter than    1544 bp
  50% of sequence in fragments shorter than    1738 bp
  55% of sequence in fragments shorter than    1957 bp
  60% of sequence in fragments shorter than    2201 bp
  65% of sequence in fragments shorter than    2481 bp
  70% of sequence in fragments shorter than    2804 bp
  75% of sequence in fragments shorter than    3214 bp
  80% of sequence in fragments shorter than    3759 bp
  85% of sequence in fragments shorter than    4538 bp
  90% of sequence in fragments shorter than    5820 bp
  95% of sequence in fragments shorter than    8852 bp

# SmlI.SphI (CTYRAG+GCATGC) double digest, 3278 thousand fragments (2727867+550952 sites), 908 bp average fragment length
   5% of sequence in fragments shorter than     313 bp
  10% of sequence in fragments shorter than     480 bp
  15% of sequence in fragments shorter than     622 bp
  20% of sequence in fragments shorter than     754 bp
  25% of sequence in fragments shorter than     887 bp
  30% of sequence in fragments shorter than    1017 bp
  35% of sequence in fragments shorter than    1151 bp
  40% of sequence in fragments shorter than    1291 bp
  45% of sequence in fragments shorter than    1433 bp
  50% of sequence in fragments shorter than    1590 bp
  55% of sequence in fragments shorter than    1758 bp
  60% of sequence in fragments shorter than    1939 bp
  65% of sequence in fragments shorter than    2135 bp
  70% of sequence in fragments shorter than    2363 bp
  75% of sequence in fragments shorter than    2630 bp
  80% of sequence in fragments shorter than    2921 bp
  85% of sequence in fragments shorter than    3329 bp
  90% of sequence in fragments shorter than    3907 bp
  95% of sequence in fragments shorter than    4994 bp

# AclI.NspI (AACGTT+RCATGY) double digest, 3276 thousand fragments (172706+3104359 sites), 909 bp average fragment length
   5% of sequence in fragments shorter than     318 bp
  10% of sequence in fragments shorter than     488 bp
  15% of sequence in fragments shorter than     639 bp
  20% of sequence in fragments shorter than     775 bp
  25% of sequence in fragments shorter than     911 bp
  30% of sequence in fragments shorter than    1046 bp
  35% of sequence in fragments shorter than    1182 bp
  40% of sequence in fragments shorter than    1325 bp
  45% of sequence in fragments shorter than    1474 bp
  50% of sequence in fragments shorter than    1632 bp
  55% of sequence in fragments shorter than    1800 bp
  60% of sequence in fragments shorter than    1983 bp
  65% of sequence in fragments shorter than    2180 bp
  70% of sequence in fragments shorter than    2410 bp
  75% of sequence in fragments shorter than    2680 bp
  80% of sequence in fragments shorter than    2999 bp
  85% of sequence in fragments shorter than    3409 bp
  90% of sequence in fragments shorter than    3959 bp
  95% of sequence in fragments shorter than    5002 bp

# PacI.StyI (TTAATTAA+CCWWGG) double digest, 3273 thousand fragments (159455+3114107 sites), 910 bp average fragment length
   5% of sequence in fragments shorter than     309 bp
  10% of sequence in fragments shorter than     476 bp
  15% of sequence in fragments shorter than     619 bp
  20% of sequence in fragments shorter than     752 bp
  25% of sequence in fragments shorter than     884 bp
  30% of sequence in fragments shorter than    1022 bp
  35% of sequence in fragments shorter than    1164 bp
  40% of sequence in fragments shorter than    1311 bp
  45% of sequence in fragments shorter than    1463 bp
  50% of sequence in fragments shorter than    1631 bp
  55% of sequence in fragments shorter than    1811 bp
  60% of sequence in fragments shorter than    2011 bp
  65% of sequence in fragments shorter than    2235 bp
  70% of sequence in fragments shorter than    2489 bp
  75% of sequence in fragments shorter than    2782 bp
  80% of sequence in fragments shorter than    3139 bp
  85% of sequence in fragments shorter than    3583 bp
  90% of sequence in fragments shorter than    4259 bp
  95% of sequence in fragments shorter than    5595 bp

# Hpy99I.StyI (CGWCG+CCWWGG) double digest, 3271 thousand fragments (157917+3114107 sites), 910 bp average fragment length
   5% of sequence in fragments shorter than     305 bp
  10% of sequence in fragments shorter than     473 bp
  15% of sequence in fragments shorter than     618 bp
  20% of sequence in fragments shorter than     753 bp
  25% of sequence in fragments shorter than     890 bp
  30% of sequence in fragments shorter than    1032 bp
  35% of sequence in fragments shorter than    1179 bp
  40% of sequence in fragments shorter than    1332 bp
  45% of sequence in fragments shorter than    1491 bp
  50% of sequence in fragments shorter than    1666 bp
  55% of sequence in fragments shorter than    1855 bp
  60% of sequence in fragments shorter than    2067 bp
  65% of sequence in fragments shorter than    2303 bp
  70% of sequence in fragments shorter than    2572 bp
  75% of sequence in fragments shorter than    2882 bp
  80% of sequence in fragments shorter than    3243 bp
  85% of sequence in fragments shorter than    3740 bp
  90% of sequence in fragments shorter than    4470 bp
  95% of sequence in fragments shorter than    5891 bp

# ScaI.SmlI (AGTACT+CTYRAG) double digest, 3271 thousand fragments (543793+2727867 sites), 910 bp average fragment length
   5% of sequence in fragments shorter than     314 bp
  10% of sequence in fragments shorter than     480 bp
  15% of sequence in fragments shorter than     621 bp
  20% of sequence in fragments shorter than     752 bp
  25% of sequence in fragments shorter than     883 bp
  30% of sequence in fragments shorter than    1012 bp
  35% of sequence in fragments shorter than    1143 bp
  40% of sequence in fragments shorter than    1280 bp
  45% of sequence in fragments shorter than    1420 bp
  50% of sequence in fragments shorter than    1573 bp
  55% of sequence in fragments shorter than    1738 bp
  60% of sequence in fragments shorter than    1917 bp
  65% of sequence in fragments shorter than    2109 bp
  70% of sequence in fragments shorter than    2334 bp
  75% of sequence in fragments shorter than    2593 bp
  80% of sequence in fragments shorter than    2876 bp
  85% of sequence in fragments shorter than    3275 bp
  90% of sequence in fragments shorter than    3847 bp
  95% of sequence in fragments shorter than    4919 bp

# NspI.PacI (RCATGY+TTAATTAA) double digest, 3263 thousand fragments (3104359+159455 sites), 913 bp average fragment length
   5% of sequence in fragments shorter than     319 bp
  10% of sequence in fragments shorter than     490 bp
  15% of sequence in fragments shorter than     641 bp
  20% of sequence in fragments shorter than     781 bp
  25% of sequence in fragments shorter than     918 bp
  30% of sequence in fragments shorter than    1056 bp
  35% of sequence in fragments shorter than    1194 bp
  40% of sequence in fragments shorter than    1339 bp
  45% of sequence in fragments shorter than    1490 bp
  50% of sequence in fragments shorter than    1650 bp
  55% of sequence in fragments shorter than    1822 bp
  60% of sequence in fragments shorter than    2009 bp
  65% of sequence in fragments shorter than    2213 bp
  70% of sequence in fragments shorter than    2450 bp
  75% of sequence in fragments shorter than    2727 bp
  80% of sequence in fragments shorter than    3059 bp
  85% of sequence in fragments shorter than    3481 bp
  90% of sequence in fragments shorter than    4090 bp
  95% of sequence in fragments shorter than    5282 bp

# Hpy99I.NspI (CGWCG+RCATGY) double digest, 3261 thousand fragments (157917+3104359 sites), 913 bp average fragment length
   5% of sequence in fragments shorter than     320 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     643 bp
  20% of sequence in fragments shorter than     782 bp
  25% of sequence in fragments shorter than     920 bp
  30% of sequence in fragments shorter than    1058 bp
  35% of sequence in fragments shorter than    1195 bp
  40% of sequence in fragments shorter than    1338 bp
  45% of sequence in fragments shorter than    1488 bp
  50% of sequence in fragments shorter than    1646 bp
  55% of sequence in fragments shorter than    1814 bp
  60% of sequence in fragments shorter than    1998 bp
  65% of sequence in fragments shorter than    2197 bp
  70% of sequence in fragments shorter than    2428 bp
  75% of sequence in fragments shorter than    2698 bp
  80% of sequence in fragments shorter than    3021 bp
  85% of sequence in fragments shorter than    3440 bp
  90% of sequence in fragments shorter than    4007 bp
  95% of sequence in fragments shorter than    5116 bp

# BfiI.HgiCI (ACTGGG+GGYRCC) double digest, 3257 thousand fragments (1706594+1550877 sites), 914 bp average fragment length
   5% of sequence in fragments shorter than     306 bp
  10% of sequence in fragments shorter than     473 bp
  15% of sequence in fragments shorter than     629 bp
  20% of sequence in fragments shorter than     769 bp
  25% of sequence in fragments shorter than     902 bp
  30% of sequence in fragments shorter than    1049 bp
  35% of sequence in fragments shorter than    1202 bp
  40% of sequence in fragments shorter than    1350 bp
  45% of sequence in fragments shorter than    1520 bp
  50% of sequence in fragments shorter than    1704 bp
  55% of sequence in fragments shorter than    1901 bp
  60% of sequence in fragments shorter than    2110 bp
  65% of sequence in fragments shorter than    2353 bp
  70% of sequence in fragments shorter than    2637 bp
  75% of sequence in fragments shorter than    2971 bp
  80% of sequence in fragments shorter than    3382 bp
  85% of sequence in fragments shorter than    3924 bp
  90% of sequence in fragments shorter than    4716 bp
  95% of sequence in fragments shorter than    6280 bp

# BfiI.BsmI (ACTGGG+GAATGC) double digest, 3255 thousand fragments (1706594+1549350 sites), 915 bp average fragment length
   5% of sequence in fragments shorter than     298 bp
  10% of sequence in fragments shorter than     478 bp
  15% of sequence in fragments shorter than     645 bp
  20% of sequence in fragments shorter than     799 bp
  25% of sequence in fragments shorter than     950 bp
  30% of sequence in fragments shorter than    1096 bp
  35% of sequence in fragments shorter than    1241 bp
  40% of sequence in fragments shorter than    1383 bp
  45% of sequence in fragments shorter than    1545 bp
  50% of sequence in fragments shorter than    1710 bp
  55% of sequence in fragments shorter than    1891 bp
  60% of sequence in fragments shorter than    2090 bp
  65% of sequence in fragments shorter than    2308 bp
  70% of sequence in fragments shorter than    2558 bp
  75% of sequence in fragments shorter than    2846 bp
  80% of sequence in fragments shorter than    3190 bp
  85% of sequence in fragments shorter than    3618 bp
  90% of sequence in fragments shorter than    4215 bp
  95% of sequence in fragments shorter than    5238 bp

# EsaBC3I.PsiI (TCGA+TTATAA) double digest, 3250 thousand fragments (1603346+1647912 sites), 916 bp average fragment length
   5% of sequence in fragments shorter than     320 bp
  10% of sequence in fragments shorter than     482 bp
  15% of sequence in fragments shorter than     626 bp
  20% of sequence in fragments shorter than     757 bp
  25% of sequence in fragments shorter than     888 bp
  30% of sequence in fragments shorter than    1021 bp
  35% of sequence in fragments shorter than    1154 bp
  40% of sequence in fragments shorter than    1292 bp
  45% of sequence in fragments shorter than    1435 bp
  50% of sequence in fragments shorter than    1590 bp
  55% of sequence in fragments shorter than    1755 bp
  60% of sequence in fragments shorter than    1933 bp
  65% of sequence in fragments shorter than    2132 bp
  70% of sequence in fragments shorter than    2359 bp
  75% of sequence in fragments shorter than    2626 bp
  80% of sequence in fragments shorter than    2941 bp
  85% of sequence in fragments shorter than    3353 bp
  90% of sequence in fragments shorter than    3925 bp
  95% of sequence in fragments shorter than    4922 bp

# AvaIII.HpaII (ATGCAT+CCGG) double digest, 3250 thousand fragments (933357+2317722 sites), 916 bp average fragment length
   5% of sequence in fragments shorter than     297 bp
  10% of sequence in fragments shorter than     503 bp
  15% of sequence in fragments shorter than     687 bp
  20% of sequence in fragments shorter than     863 bp
  25% of sequence in fragments shorter than    1038 bp
  30% of sequence in fragments shorter than    1216 bp
  35% of sequence in fragments shorter than    1399 bp
  40% of sequence in fragments shorter than    1590 bp
  45% of sequence in fragments shorter than    1793 bp
  50% of sequence in fragments shorter than    2011 bp
  55% of sequence in fragments shorter than    2238 bp
  60% of sequence in fragments shorter than    2500 bp
  65% of sequence in fragments shorter than    2788 bp
  70% of sequence in fragments shorter than    3123 bp
  75% of sequence in fragments shorter than    3517 bp
  80% of sequence in fragments shorter than    4005 bp
  85% of sequence in fragments shorter than    4628 bp
  90% of sequence in fragments shorter than    5520 bp
  95% of sequence in fragments shorter than    7185 bp

# SnaBI.StyI (TACGTA+CCWWGG) double digest, 3245 thousand fragments (132299+3114107 sites), 918 bp average fragment length
   5% of sequence in fragments shorter than     311 bp
  10% of sequence in fragments shorter than     479 bp
  15% of sequence in fragments shorter than     622 bp
  20% of sequence in fragments shorter than     754 bp
  25% of sequence in fragments shorter than     889 bp
  30% of sequence in fragments shorter than    1029 bp
  35% of sequence in fragments shorter than    1174 bp
  40% of sequence in fragments shorter than    1323 bp
  45% of sequence in fragments shorter than    1479 bp
  50% of sequence in fragments shorter than    1649 bp
  55% of sequence in fragments shorter than    1831 bp
  60% of sequence in fragments shorter than    2035 bp
  65% of sequence in fragments shorter than    2261 bp
  70% of sequence in fragments shorter than    2520 bp
  75% of sequence in fragments shorter than    2817 bp
  80% of sequence in fragments shorter than    3161 bp
  85% of sequence in fragments shorter than    3630 bp
  90% of sequence in fragments shorter than    4319 bp
  95% of sequence in fragments shorter than    5666 bp

# ApaLI.AvaII (GTGCAC+GGWCC) double digest, 3243 thousand fragments (496255+2747690 sites), 918 bp average fragment length
   5% of sequence in fragments shorter than     300 bp
  10% of sequence in fragments shorter than     470 bp
  15% of sequence in fragments shorter than     634 bp
  20% of sequence in fragments shorter than     792 bp
  25% of sequence in fragments shorter than     939 bp
  30% of sequence in fragments shorter than    1103 bp
  35% of sequence in fragments shorter than    1273 bp
  40% of sequence in fragments shorter than    1450 bp
  45% of sequence in fragments shorter than    1639 bp
  50% of sequence in fragments shorter than    1818 bp
  55% of sequence in fragments shorter than    2034 bp
  60% of sequence in fragments shorter than    2275 bp
  65% of sequence in fragments shorter than    2545 bp
  70% of sequence in fragments shorter than    2840 bp
  75% of sequence in fragments shorter than    3202 bp
  80% of sequence in fragments shorter than    3640 bp
  85% of sequence in fragments shorter than    4214 bp
  90% of sequence in fragments shorter than    5045 bp
  95% of sequence in fragments shorter than    6548 bp

# HpaII.StuI (CCGG+AGGCCT) double digest, 3242 thousand fragments (2317722+925729 sites), 918 bp average fragment length
   5% of sequence in fragments shorter than     284 bp
  10% of sequence in fragments shorter than     482 bp
  15% of sequence in fragments shorter than     684 bp
  20% of sequence in fragments shorter than     862 bp
  25% of sequence in fragments shorter than    1063 bp
  30% of sequence in fragments shorter than    1274 bp
  35% of sequence in fragments shorter than    1490 bp
  40% of sequence in fragments shorter than    1728 bp
  45% of sequence in fragments shorter than    1978 bp
  50% of sequence in fragments shorter than    2250 bp
  55% of sequence in fragments shorter than    2563 bp
  60% of sequence in fragments shorter than    2905 bp
  65% of sequence in fragments shorter than    3292 bp
  70% of sequence in fragments shorter than    3735 bp
  75% of sequence in fragments shorter than    4258 bp
  80% of sequence in fragments shorter than    4899 bp
  85% of sequence in fragments shorter than    5718 bp
  90% of sequence in fragments shorter than    6855 bp
  95% of sequence in fragments shorter than    8846 bp

# AflIII.HhaI (ACRYGT+GCGC) double digest, 3241 thousand fragments (1485394+1756502 sites), 919 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     514 bp
  15% of sequence in fragments shorter than     690 bp
  20% of sequence in fragments shorter than     863 bp
  25% of sequence in fragments shorter than    1034 bp
  30% of sequence in fragments shorter than    1206 bp
  35% of sequence in fragments shorter than    1382 bp
  40% of sequence in fragments shorter than    1565 bp
  45% of sequence in fragments shorter than    1756 bp
  50% of sequence in fragments shorter than    1960 bp
  55% of sequence in fragments shorter than    2181 bp
  60% of sequence in fragments shorter than    2424 bp
  65% of sequence in fragments shorter than    2693 bp
  70% of sequence in fragments shorter than    2998 bp
  75% of sequence in fragments shorter than    3355 bp
  80% of sequence in fragments shorter than    3769 bp
  85% of sequence in fragments shorter than    4310 bp
  90% of sequence in fragments shorter than    5046 bp
  95% of sequence in fragments shorter than    6267 bp

# NaeI.StyI (GCCGGC+CCWWGG) double digest, 3239 thousand fragments (125692+3114107 sites), 919 bp average fragment length
   5% of sequence in fragments shorter than     308 bp
  10% of sequence in fragments shorter than     477 bp
  15% of sequence in fragments shorter than     624 bp
  20% of sequence in fragments shorter than     756 bp
  25% of sequence in fragments shorter than     900 bp
  30% of sequence in fragments shorter than    1044 bp
  35% of sequence in fragments shorter than    1195 bp
  40% of sequence in fragments shorter than    1350 bp
  45% of sequence in fragments shorter than    1510 bp
  50% of sequence in fragments shorter than    1688 bp
  55% of sequence in fragments shorter than    1880 bp
  60% of sequence in fragments shorter than    2095 bp
  65% of sequence in fragments shorter than    2335 bp
  70% of sequence in fragments shorter than    2607 bp
  75% of sequence in fragments shorter than    2924 bp
  80% of sequence in fragments shorter than    3287 bp
  85% of sequence in fragments shorter than    3795 bp
  90% of sequence in fragments shorter than    4543 bp
  95% of sequence in fragments shorter than    6029 bp

# HindIII.SspI (AAGCTT+AATATT) double digest, 3237 thousand fragments (860365+2377267 sites), 920 bp average fragment length
   5% of sequence in fragments shorter than     304 bp
  10% of sequence in fragments shorter than     477 bp
  15% of sequence in fragments shorter than     627 bp
  20% of sequence in fragments shorter than     772 bp
  25% of sequence in fragments shorter than     920 bp
  30% of sequence in fragments shorter than    1071 bp
  35% of sequence in fragments shorter than    1226 bp
  40% of sequence in fragments shorter than    1383 bp
  45% of sequence in fragments shorter than    1553 bp
  50% of sequence in fragments shorter than    1733 bp
  55% of sequence in fragments shorter than    1936 bp
  60% of sequence in fragments shorter than    2169 bp
  65% of sequence in fragments shorter than    2434 bp
  70% of sequence in fragments shorter than    2734 bp
  75% of sequence in fragments shorter than    3096 bp
  80% of sequence in fragments shorter than    3561 bp
  85% of sequence in fragments shorter than    4196 bp
  90% of sequence in fragments shorter than    5201 bp
  95% of sequence in fragments shorter than    7406 bp

# NspI.SnaBI (RCATGY+TACGTA) double digest, 3236 thousand fragments (3104359+132299 sites), 920 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     495 bp
  15% of sequence in fragments shorter than     648 bp
  20% of sequence in fragments shorter than     789 bp
  25% of sequence in fragments shorter than     928 bp
  30% of sequence in fragments shorter than    1065 bp
  35% of sequence in fragments shorter than    1206 bp
  40% of sequence in fragments shorter than    1352 bp
  45% of sequence in fragments shorter than    1502 bp
  50% of sequence in fragments shorter than    1664 bp
  55% of sequence in fragments shorter than    1835 bp
  60% of sequence in fragments shorter than    2023 bp
  65% of sequence in fragments shorter than    2228 bp
  70% of sequence in fragments shorter than    2465 bp
  75% of sequence in fragments shorter than    2742 bp
  80% of sequence in fragments shorter than    3072 bp
  85% of sequence in fragments shorter than    3490 bp
  90% of sequence in fragments shorter than    4097 bp
  95% of sequence in fragments shorter than    5264 bp

# StyI.XhoI (CCWWGG+CTCGAG) double digest, 3234 thousand fragments (3114107+121308 sites), 921 bp average fragment length
   5% of sequence in fragments shorter than     310 bp
  10% of sequence in fragments shorter than     478 bp
  15% of sequence in fragments shorter than     624 bp
  20% of sequence in fragments shorter than     755 bp
  25% of sequence in fragments shorter than     897 bp
  30% of sequence in fragments shorter than    1040 bp
  35% of sequence in fragments shorter than    1187 bp
  40% of sequence in fragments shorter than    1340 bp
  45% of sequence in fragments shorter than    1498 bp
  50% of sequence in fragments shorter than    1672 bp
  55% of sequence in fragments shorter than    1861 bp
  60% of sequence in fragments shorter than    2072 bp
  65% of sequence in fragments shorter than    2307 bp
  70% of sequence in fragments shorter than    2576 bp
  75% of sequence in fragments shorter than    2867 bp
  80% of sequence in fragments shorter than    3240 bp
  85% of sequence in fragments shorter than    3737 bp
  90% of sequence in fragments shorter than    4474 bp
  95% of sequence in fragments shorter than    5909 bp

# NaeI.NspI (GCCGGC+RCATGY) double digest, 3229 thousand fragments (125692+3104359 sites), 922 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     495 bp
  15% of sequence in fragments shorter than     649 bp
  20% of sequence in fragments shorter than     790 bp
  25% of sequence in fragments shorter than     929 bp
  30% of sequence in fragments shorter than    1066 bp
  35% of sequence in fragments shorter than    1206 bp
  40% of sequence in fragments shorter than    1351 bp
  45% of sequence in fragments shorter than    1501 bp
  50% of sequence in fragments shorter than    1661 bp
  55% of sequence in fragments shorter than    1831 bp
  60% of sequence in fragments shorter than    2017 bp
  65% of sequence in fragments shorter than    2220 bp
  70% of sequence in fragments shorter than    2455 bp
  75% of sequence in fragments shorter than    2730 bp
  80% of sequence in fragments shorter than    3056 bp
  85% of sequence in fragments shorter than    3474 bp
  90% of sequence in fragments shorter than    4062 bp
  95% of sequence in fragments shorter than    5212 bp

# SspI.XbaI (AATATT+TCTAGA) double digest, 3227 thousand fragments (2377267+850998 sites), 923 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     480 bp
  15% of sequence in fragments shorter than     631 bp
  20% of sequence in fragments shorter than     775 bp
  25% of sequence in fragments shorter than     911 bp
  30% of sequence in fragments shorter than    1058 bp
  35% of sequence in fragments shorter than    1209 bp
  40% of sequence in fragments shorter than    1366 bp
  45% of sequence in fragments shorter than    1524 bp
  50% of sequence in fragments shorter than    1711 bp
  55% of sequence in fragments shorter than    1909 bp
  60% of sequence in fragments shorter than    2138 bp
  65% of sequence in fragments shorter than    2395 bp
  70% of sequence in fragments shorter than    2705 bp
  75% of sequence in fragments shorter than    3078 bp
  80% of sequence in fragments shorter than    3549 bp
  85% of sequence in fragments shorter than    4201 bp
  90% of sequence in fragments shorter than    5210 bp
  95% of sequence in fragments shorter than    7379 bp

# NspI.XhoI (RCATGY+CTCGAG) double digest, 3225 thousand fragments (3104359+121308 sites), 923 bp average fragment length
   5% of sequence in fragments shorter than     323 bp
  10% of sequence in fragments shorter than     495 bp
  15% of sequence in fragments shorter than     648 bp
  20% of sequence in fragments shorter than     789 bp
  25% of sequence in fragments shorter than     928 bp
  30% of sequence in fragments shorter than    1065 bp
  35% of sequence in fragments shorter than    1205 bp
  40% of sequence in fragments shorter than    1350 bp
  45% of sequence in fragments shorter than    1502 bp
  50% of sequence in fragments shorter than    1662 bp
  55% of sequence in fragments shorter than    1832 bp
  60% of sequence in fragments shorter than    2018 bp
  65% of sequence in fragments shorter than    2221 bp
  70% of sequence in fragments shorter than    2457 bp
  75% of sequence in fragments shorter than    2731 bp
  80% of sequence in fragments shorter than    3057 bp
  85% of sequence in fragments shorter than    3475 bp
  90% of sequence in fragments shorter than    4050 bp
  95% of sequence in fragments shorter than    5094 bp

# EcoRI.SspI (GAATTC+AATATT) double digest, 3224 thousand fragments (847401+2377267 sites), 924 bp average fragment length
   5% of sequence in fragments shorter than     311 bp
  10% of sequence in fragments shorter than     474 bp
  15% of sequence in fragments shorter than     628 bp
  20% of sequence in fragments shorter than     768 bp
  25% of sequence in fragments shorter than     916 bp
  30% of sequence in fragments shorter than    1062 bp
  35% of sequence in fragments shorter than    1220 bp
  40% of sequence in fragments shorter than    1382 bp
  45% of sequence in fragments shorter than    1546 bp
  50% of sequence in fragments shorter than    1735 bp
  55% of sequence in fragments shorter than    1940 bp
  60% of sequence in fragments shorter than    2173 bp
  65% of sequence in fragments shorter than    2435 bp
  70% of sequence in fragments shorter than    2741 bp
  75% of sequence in fragments shorter than    3102 bp
  80% of sequence in fragments shorter than    3561 bp
  85% of sequence in fragments shorter than    4185 bp
  90% of sequence in fragments shorter than    5142 bp
  95% of sequence in fragments shorter than    7130 bp

# ApaLI.SmlI (GTGCAC+CTYRAG) double digest, 3223 thousand fragments (496255+2727867 sites), 924 bp average fragment length
   5% of sequence in fragments shorter than     318 bp
  10% of sequence in fragments shorter than     489 bp
  15% of sequence in fragments shorter than     632 bp
  20% of sequence in fragments shorter than     768 bp
  25% of sequence in fragments shorter than     903 bp
  30% of sequence in fragments shorter than    1034 bp
  35% of sequence in fragments shorter than    1170 bp
  40% of sequence in fragments shorter than    1311 bp
  45% of sequence in fragments shorter than    1455 bp
  50% of sequence in fragments shorter than    1613 bp
  55% of sequence in fragments shorter than    1777 bp
  60% of sequence in fragments shorter than    1951 bp
  65% of sequence in fragments shorter than    2149 bp
  70% of sequence in fragments shorter than    2380 bp
  75% of sequence in fragments shorter than    2642 bp
  80% of sequence in fragments shorter than    2940 bp
  85% of sequence in fragments shorter than    3351 bp
  90% of sequence in fragments shorter than    3930 bp
  95% of sequence in fragments shorter than    4994 bp

# HpaII.NdeI (CCGG+CATATG) double digest, 3220 thousand fragments (2317722+903360 sites), 925 bp average fragment length
   5% of sequence in fragments shorter than     297 bp
  10% of sequence in fragments shorter than     507 bp
  15% of sequence in fragments shorter than     694 bp
  20% of sequence in fragments shorter than     866 bp
  25% of sequence in fragments shorter than    1041 bp
  30% of sequence in fragments shorter than    1207 bp
  35% of sequence in fragments shorter than    1390 bp
  40% of sequence in fragments shorter than    1581 bp
  45% of sequence in fragments shorter than    1782 bp
  50% of sequence in fragments shorter than    1998 bp
  55% of sequence in fragments shorter than    2230 bp
  60% of sequence in fragments shorter than    2490 bp
  65% of sequence in fragments shorter than    2773 bp
  70% of sequence in fragments shorter than    3098 bp
  75% of sequence in fragments shorter than    3481 bp
  80% of sequence in fragments shorter than    3944 bp
  85% of sequence in fragments shorter than    4539 bp
  90% of sequence in fragments shorter than    5420 bp
  95% of sequence in fragments shorter than    7130 bp

# ApaI.AvaII (GGGCCC+GGWCC) double digest, 3207 thousand fragments (460339+2747690 sites), 929 bp average fragment length
   5% of sequence in fragments shorter than     292 bp
  10% of sequence in fragments shorter than     472 bp
  15% of sequence in fragments shorter than     651 bp
  20% of sequence in fragments shorter than     827 bp
  25% of sequence in fragments shorter than     990 bp
  30% of sequence in fragments shorter than    1178 bp
  35% of sequence in fragments shorter than    1372 bp
  40% of sequence in fragments shorter than    1574 bp
  45% of sequence in fragments shorter than    1787 bp
  50% of sequence in fragments shorter than    2016 bp
  55% of sequence in fragments shorter than    2266 bp
  60% of sequence in fragments shorter than    2544 bp
  65% of sequence in fragments shorter than    2847 bp
  70% of sequence in fragments shorter than    3204 bp
  75% of sequence in fragments shorter than    3625 bp
  80% of sequence in fragments shorter than    4144 bp
  85% of sequence in fragments shorter than    4823 bp
  90% of sequence in fragments shorter than    5799 bp
  95% of sequence in fragments shorter than    7605 bp

# ClaI.StyI (ATCGAT+CCWWGG) double digest, 3200 thousand fragments (86477+3114107 sites), 931 bp average fragment length
   5% of sequence in fragments shorter than     315 bp
  10% of sequence in fragments shorter than     485 bp
  15% of sequence in fragments shorter than     631 bp
  20% of sequence in fragments shorter than     761 bp
  25% of sequence in fragments shorter than     903 bp
  30% of sequence in fragments shorter than    1046 bp
  35% of sequence in fragments shorter than    1194 bp
  40% of sequence in fragments shorter than    1345 bp
  45% of sequence in fragments shorter than    1502 bp
  50% of sequence in fragments shorter than    1676 bp
  55% of sequence in fragments shorter than    1862 bp
  60% of sequence in fragments shorter than    2069 bp
  65% of sequence in fragments shorter than    2300 bp
  70% of sequence in fragments shorter than    2561 bp
  75% of sequence in fragments shorter than    2868 bp
  80% of sequence in fragments shorter than    3229 bp
  85% of sequence in fragments shorter than    3716 bp
  90% of sequence in fragments shorter than    4437 bp
  95% of sequence in fragments shorter than    5861 bp

# HgiCI.PsiI (GGYRCC+TTATAA) double digest, 3198 thousand fragments (1550877+1647912 sites), 931 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     637 bp
  20% of sequence in fragments shorter than     772 bp
  25% of sequence in fragments shorter than     895 bp
  30% of sequence in fragments shorter than    1030 bp
  35% of sequence in fragments shorter than    1164 bp
  40% of sequence in fragments shorter than    1291 bp
  45% of sequence in fragments shorter than    1435 bp
  50% of sequence in fragments shorter than    1589 bp
  55% of sequence in fragments shorter than    1753 bp
  60% of sequence in fragments shorter than    1933 bp
  65% of sequence in fragments shorter than    2125 bp
  70% of sequence in fragments shorter than    2352 bp
  75% of sequence in fragments shorter than    2617 bp
  80% of sequence in fragments shorter than    2931 bp
  85% of sequence in fragments shorter than    3344 bp
  90% of sequence in fragments shorter than    3954 bp
  95% of sequence in fragments shorter than    5171 bp

# BsmI.PsiI (GAATGC+TTATAA) double digest, 3196 thousand fragments (1549350+1647912 sites), 932 bp average fragment length
   5% of sequence in fragments shorter than     298 bp
  10% of sequence in fragments shorter than     487 bp
  15% of sequence in fragments shorter than     660 bp
  20% of sequence in fragments shorter than     820 bp
  25% of sequence in fragments shorter than     975 bp
  30% of sequence in fragments shorter than    1125 bp
  35% of sequence in fragments shorter than    1282 bp
  40% of sequence in fragments shorter than    1431 bp
  45% of sequence in fragments shorter than    1597 bp
  50% of sequence in fragments shorter than    1777 bp
  55% of sequence in fragments shorter than    1973 bp
  60% of sequence in fragments shorter than    2188 bp
  65% of sequence in fragments shorter than    2429 bp
  70% of sequence in fragments shorter than    2703 bp
  75% of sequence in fragments shorter than    3025 bp
  80% of sequence in fragments shorter than    3422 bp
  85% of sequence in fragments shorter than    3942 bp
  90% of sequence in fragments shorter than    4688 bp
  95% of sequence in fragments shorter than    6037 bp

# FauI.HhaI (CCCGC+GCGC) double digest, 3195 thousand fragments (1439772+1756502 sites), 932 bp average fragment length
   5% of sequence in fragments shorter than     315 bp
  10% of sequence in fragments shorter than     583 bp
  15% of sequence in fragments shorter than     836 bp
  20% of sequence in fragments shorter than    1099 bp
  25% of sequence in fragments shorter than    1384 bp
  30% of sequence in fragments shorter than    1681 bp
  35% of sequence in fragments shorter than    1997 bp
  40% of sequence in fragments shorter than    2351 bp
  45% of sequence in fragments shorter than    2729 bp
  50% of sequence in fragments shorter than    3152 bp
  55% of sequence in fragments shorter than    3618 bp
  60% of sequence in fragments shorter than    4139 bp
  65% of sequence in fragments shorter than    4726 bp
  70% of sequence in fragments shorter than    5413 bp
  75% of sequence in fragments shorter than    6216 bp
  80% of sequence in fragments shorter than    7228 bp
  85% of sequence in fragments shorter than    8519 bp
  90% of sequence in fragments shorter than   10356 bp
  95% of sequence in fragments shorter than   13595 bp

# BspMI.HhaI (ACCTGC+GCGC) double digest, 3194 thousand fragments (1438129+1756502 sites), 932 bp average fragment length
   5% of sequence in fragments shorter than     300 bp
  10% of sequence in fragments shorter than     510 bp
  15% of sequence in fragments shorter than     699 bp
  20% of sequence in fragments shorter than     891 bp
  25% of sequence in fragments shorter than    1079 bp
  30% of sequence in fragments shorter than    1276 bp
  35% of sequence in fragments shorter than    1483 bp
  40% of sequence in fragments shorter than    1705 bp
  45% of sequence in fragments shorter than    1933 bp
  50% of sequence in fragments shorter than    2177 bp
  55% of sequence in fragments shorter than    2454 bp
  60% of sequence in fragments shorter than    2759 bp
  65% of sequence in fragments shorter than    3101 bp
  70% of sequence in fragments shorter than    3444 bp
  75% of sequence in fragments shorter than    3900 bp
  80% of sequence in fragments shorter than    4461 bp
  85% of sequence in fragments shorter than    5169 bp
  90% of sequence in fragments shorter than    6158 bp
  95% of sequence in fragments shorter than    7882 bp

# AvaII.EcoRV (GGWCC+GATATC) double digest, 3193 thousand fragments (2747690+446473 sites), 933 bp average fragment length
   5% of sequence in fragments shorter than     308 bp
  10% of sequence in fragments shorter than     482 bp
  15% of sequence in fragments shorter than     646 bp
  20% of sequence in fragments shorter than     796 bp
  25% of sequence in fragments shorter than     944 bp
  30% of sequence in fragments shorter than    1104 bp
  35% of sequence in fragments shorter than    1269 bp
  40% of sequence in fragments shorter than    1441 bp
  45% of sequence in fragments shorter than    1625 bp
  50% of sequence in fragments shorter than    1815 bp
  55% of sequence in fragments shorter than    2025 bp
  60% of sequence in fragments shorter than    2256 bp
  65% of sequence in fragments shorter than    2507 bp
  70% of sequence in fragments shorter than    2793 bp
  75% of sequence in fragments shorter than    3131 bp
  80% of sequence in fragments shorter than    3542 bp
  85% of sequence in fragments shorter than    4073 bp
  90% of sequence in fragments shorter than    4822 bp
  95% of sequence in fragments shorter than    6121 bp

# AflIII.BfiI (ACRYGT+ACTGGG) double digest, 3191 thousand fragments (1485394+1706594 sites), 933 bp average fragment length
   5% of sequence in fragments shorter than     323 bp
  10% of sequence in fragments shorter than     495 bp
  15% of sequence in fragments shorter than     652 bp
  20% of sequence in fragments shorter than     797 bp
  25% of sequence in fragments shorter than     940 bp
  30% of sequence in fragments shorter than    1079 bp
  35% of sequence in fragments shorter than    1224 bp
  40% of sequence in fragments shorter than    1375 bp
  45% of sequence in fragments shorter than    1532 bp
  50% of sequence in fragments shorter than    1700 bp
  55% of sequence in fragments shorter than    1876 bp
  60% of sequence in fragments shorter than    2067 bp
  65% of sequence in fragments shorter than    2264 bp
  70% of sequence in fragments shorter than    2508 bp
  75% of sequence in fragments shorter than    2780 bp
  80% of sequence in fragments shorter than    3093 bp
  85% of sequence in fragments shorter than    3515 bp
  90% of sequence in fragments shorter than    4129 bp
  95% of sequence in fragments shorter than    5201 bp

# ClaI.NspI (ATCGAT+RCATGY) double digest, 3190 thousand fragments (86477+3104359 sites), 934 bp average fragment length
   5% of sequence in fragments shorter than     326 bp
  10% of sequence in fragments shorter than     501 bp
  15% of sequence in fragments shorter than     657 bp
  20% of sequence in fragments shorter than     799 bp
  25% of sequence in fragments shorter than     940 bp
  30% of sequence in fragments shorter than    1079 bp
  35% of sequence in fragments shorter than    1220 bp
  40% of sequence in fragments shorter than    1368 bp
  45% of sequence in fragments shorter than    1520 bp
  50% of sequence in fragments shorter than    1682 bp
  55% of sequence in fragments shorter than    1854 bp
  60% of sequence in fragments shorter than    2039 bp
  65% of sequence in fragments shorter than    2244 bp
  70% of sequence in fragments shorter than    2482 bp
  75% of sequence in fragments shorter than    2758 bp
  80% of sequence in fragments shorter than    3094 bp
  85% of sequence in fragments shorter than    3515 bp
  90% of sequence in fragments shorter than    4133 bp
  95% of sequence in fragments shorter than    5326 bp

# ApaI.SmlI (GGGCCC+CTYRAG) double digest, 3187 thousand fragments (460339+2727867 sites), 934 bp average fragment length
   5% of sequence in fragments shorter than     316 bp
  10% of sequence in fragments shorter than     487 bp
  15% of sequence in fragments shorter than     632 bp
  20% of sequence in fragments shorter than     771 bp
  25% of sequence in fragments shorter than     909 bp
  30% of sequence in fragments shorter than    1043 bp
  35% of sequence in fragments shorter than    1184 bp
  40% of sequence in fragments shorter than    1331 bp
  45% of sequence in fragments shorter than    1481 bp
  50% of sequence in fragments shorter than    1648 bp
  55% of sequence in fragments shorter than    1826 bp
  60% of sequence in fragments shorter than    2019 bp
  65% of sequence in fragments shorter than    2228 bp
  70% of sequence in fragments shorter than    2472 bp
  75% of sequence in fragments shorter than    2730 bp
  80% of sequence in fragments shorter than    3068 bp
  85% of sequence in fragments shorter than    3515 bp
  90% of sequence in fragments shorter than    4143 bp
  95% of sequence in fragments shorter than    5356 bp

# AatII.StyI (GACGTC+CCWWGG) double digest, 3184 thousand fragments (71089+3114107 sites), 935 bp average fragment length
   5% of sequence in fragments shorter than     315 bp
  10% of sequence in fragments shorter than     486 bp
  15% of sequence in fragments shorter than     634 bp
  20% of sequence in fragments shorter than     766 bp
  25% of sequence in fragments shorter than     910 bp
  30% of sequence in fragments shorter than    1055 bp
  35% of sequence in fragments shorter than    1205 bp
  40% of sequence in fragments shorter than    1360 bp
  45% of sequence in fragments shorter than    1519 bp
  50% of sequence in fragments shorter than    1697 bp
  55% of sequence in fragments shorter than    1888 bp
  60% of sequence in fragments shorter than    2102 bp
  65% of sequence in fragments shorter than    2340 bp
  70% of sequence in fragments shorter than    2610 bp
  75% of sequence in fragments shorter than    2925 bp
  80% of sequence in fragments shorter than    3286 bp
  85% of sequence in fragments shorter than    3789 bp
  90% of sequence in fragments shorter than    4529 bp
  95% of sequence in fragments shorter than    5990 bp

# SacII.StyI (CCGCGG+CCWWGG) double digest, 3180 thousand fragments (66901+3114107 sites), 936 bp average fragment length
   5% of sequence in fragments shorter than     314 bp
  10% of sequence in fragments shorter than     486 bp
  15% of sequence in fragments shorter than     635 bp
  20% of sequence in fragments shorter than     769 bp
  25% of sequence in fragments shorter than     915 bp
  30% of sequence in fragments shorter than    1062 bp
  35% of sequence in fragments shorter than    1213 bp
  40% of sequence in fragments shorter than    1370 bp
  45% of sequence in fragments shorter than    1532 bp
  50% of sequence in fragments shorter than    1712 bp
  55% of sequence in fragments shorter than    1905 bp
  60% of sequence in fragments shorter than    2123 bp
  65% of sequence in fragments shorter than    2365 bp
  70% of sequence in fragments shorter than    2639 bp
  75% of sequence in fragments shorter than    2958 bp
  80% of sequence in fragments shorter than    3319 bp
  85% of sequence in fragments shorter than    3834 bp
  90% of sequence in fragments shorter than    4587 bp
  95% of sequence in fragments shorter than    6087 bp

# HindIII.HpaII (AAGCTT+CCGG) double digest, 3177 thousand fragments (860365+2317722 sites), 937 bp average fragment length
   5% of sequence in fragments shorter than     301 bp
  10% of sequence in fragments shorter than     513 bp
  15% of sequence in fragments shorter than     701 bp
  20% of sequence in fragments shorter than     886 bp
  25% of sequence in fragments shorter than    1069 bp
  30% of sequence in fragments shorter than    1253 bp
  35% of sequence in fragments shorter than    1443 bp
  40% of sequence in fragments shorter than    1645 bp
  45% of sequence in fragments shorter than    1851 bp
  50% of sequence in fragments shorter than    2069 bp
  55% of sequence in fragments shorter than    2315 bp
  60% of sequence in fragments shorter than    2590 bp
  65% of sequence in fragments shorter than    2897 bp
  70% of sequence in fragments shorter than    3245 bp
  75% of sequence in fragments shorter than    3654 bp
  80% of sequence in fragments shorter than    4153 bp
  85% of sequence in fragments shorter than    4804 bp
  90% of sequence in fragments shorter than    5736 bp
  95% of sequence in fragments shorter than    7449 bp

# AatII.NspI (GACGTC+RCATGY) double digest, 3174 thousand fragments (71089+3104359 sites), 938 bp average fragment length
   5% of sequence in fragments shorter than     327 bp
  10% of sequence in fragments shorter than     503 bp
  15% of sequence in fragments shorter than     659 bp
  20% of sequence in fragments shorter than     802 bp
  25% of sequence in fragments shorter than     944 bp
  30% of sequence in fragments shorter than    1084 bp
  35% of sequence in fragments shorter than    1225 bp
  40% of sequence in fragments shorter than    1374 bp
  45% of sequence in fragments shorter than    1528 bp
  50% of sequence in fragments shorter than    1690 bp
  55% of sequence in fragments shorter than    1864 bp
  60% of sequence in fragments shorter than    2055 bp
  65% of sequence in fragments shorter than    2261 bp
  70% of sequence in fragments shorter than    2503 bp
  75% of sequence in fragments shorter than    2780 bp
  80% of sequence in fragments shorter than    3115 bp
  85% of sequence in fragments shorter than    3538 bp
  90% of sequence in fragments shorter than    4153 bp
  95% of sequence in fragments shorter than    5338 bp

# EcoRV.SmlI (GATATC+CTYRAG) double digest, 3173 thousand fragments (446473+2727867 sites), 938 bp average fragment length
   5% of sequence in fragments shorter than     324 bp
  10% of sequence in fragments shorter than     497 bp
  15% of sequence in fragments shorter than     641 bp
  20% of sequence in fragments shorter than     773 bp
  25% of sequence in fragments shorter than     908 bp
  30% of sequence in fragments shorter than    1030 bp
  35% of sequence in fragments shorter than    1164 bp
  40% of sequence in fragments shorter than    1305 bp
  45% of sequence in fragments shorter than    1448 bp
  50% of sequence in fragments shorter than    1605 bp
  55% of sequence in fragments shorter than    1761 bp
  60% of sequence in fragments shorter than    1941 bp
  65% of sequence in fragments shorter than    2134 bp
  70% of sequence in fragments shorter than    2358 bp
  75% of sequence in fragments shorter than    2617 bp
  80% of sequence in fragments shorter than    2924 bp
  85% of sequence in fragments shorter than    3326 bp
  90% of sequence in fragments shorter than    3896 bp
  95% of sequence in fragments shorter than    4912 bp

# PspXI.StyI (VCTCGAGB+CCWWGG) double digest, 3172 thousand fragments (59193+3114107 sites), 939 bp average fragment length
   5% of sequence in fragments shorter than     316 bp
  10% of sequence in fragments shorter than     488 bp
  15% of sequence in fragments shorter than     636 bp
  20% of sequence in fragments shorter than     769 bp
  25% of sequence in fragments shorter than     914 bp
  30% of sequence in fragments shorter than    1059 bp
  35% of sequence in fragments shorter than    1210 bp
  40% of sequence in fragments shorter than    1365 bp
  45% of sequence in fragments shorter than    1526 bp
  50% of sequence in fragments shorter than    1704 bp
  55% of sequence in fragments shorter than    1896 bp
  60% of sequence in fragments shorter than    2111 bp
  65% of sequence in fragments shorter than    2351 bp
  70% of sequence in fragments shorter than    2623 bp
  75% of sequence in fragments shorter than    2939 bp
  80% of sequence in fragments shorter than    3299 bp
  85% of sequence in fragments shorter than    3807 bp
  90% of sequence in fragments shorter than    4551 bp
  95% of sequence in fragments shorter than    6023 bp

# NspI.SacII (RCATGY+CCGCGG) double digest, 3170 thousand fragments (3104359+66901 sites), 939 bp average fragment length
   5% of sequence in fragments shorter than     328 bp
  10% of sequence in fragments shorter than     505 bp
  15% of sequence in fragments shorter than     662 bp
  20% of sequence in fragments shorter than     806 bp
  25% of sequence in fragments shorter than     948 bp
  30% of sequence in fragments shorter than    1088 bp
  35% of sequence in fragments shorter than    1230 bp
  40% of sequence in fragments shorter than    1379 bp
  45% of sequence in fragments shorter than    1533 bp
  50% of sequence in fragments shorter than    1695 bp
  55% of sequence in fragments shorter than    1869 bp
  60% of sequence in fragments shorter than    2060 bp
  65% of sequence in fragments shorter than    2266 bp
  70% of sequence in fragments shorter than    2508 bp
  75% of sequence in fragments shorter than    2784 bp
  80% of sequence in fragments shorter than    3118 bp
  85% of sequence in fragments shorter than    3540 bp
  90% of sequence in fragments shorter than    4148 bp
  95% of sequence in fragments shorter than    5328 bp

# HpaII.XbaI (CCGG+TCTAGA) double digest, 3168 thousand fragments (2317722+850998 sites), 940 bp average fragment length
   5% of sequence in fragments shorter than     307 bp
  10% of sequence in fragments shorter than     516 bp
  15% of sequence in fragments shorter than     701 bp
  20% of sequence in fragments shorter than     879 bp
  25% of sequence in fragments shorter than    1060 bp
  30% of sequence in fragments shorter than    1248 bp
  35% of sequence in fragments shorter than    1422 bp
  40% of sequence in fragments shorter than    1621 bp
  45% of sequence in fragments shorter than    1833 bp
  50% of sequence in fragments shorter than    2058 bp
  55% of sequence in fragments shorter than    2297 bp
  60% of sequence in fragments shorter than    2571 bp
  65% of sequence in fragments shorter than    2871 bp
  70% of sequence in fragments shorter than    3212 bp
  75% of sequence in fragments shorter than    3612 bp
  80% of sequence in fragments shorter than    4102 bp
  85% of sequence in fragments shorter than    4737 bp
  90% of sequence in fragments shorter than    5640 bp
  95% of sequence in fragments shorter than    7237 bp

# AgeI.StyI (ACCGGT+CCWWGG) double digest, 3167 thousand fragments (53386+3114107 sites), 940 bp average fragment length
   5% of sequence in fragments shorter than     317 bp
  10% of sequence in fragments shorter than     489 bp
  15% of sequence in fragments shorter than     637 bp
  20% of sequence in fragments shorter than     769 bp
  25% of sequence in fragments shorter than     914 bp
  30% of sequence in fragments shorter than    1060 bp
  35% of sequence in fragments shorter than    1210 bp
  40% of sequence in fragments shorter than    1366 bp
  45% of sequence in fragments shorter than    1526 bp
  50% of sequence in fragments shorter than    1704 bp
  55% of sequence in fragments shorter than    1895 bp
  60% of sequence in fragments shorter than    2109 bp
  65% of sequence in fragments shorter than    2347 bp
  70% of sequence in fragments shorter than    2604 bp
  75% of sequence in fragments shorter than    2917 bp
  80% of sequence in fragments shorter than    3291 bp
  85% of sequence in fragments shorter than    3797 bp
  90% of sequence in fragments shorter than    4542 bp
  95% of sequence in fragments shorter than    6016 bp

# EcoRI.HpaII (GAATTC+CCGG) double digest, 3164 thousand fragments (847401+2317722 sites), 941 bp average fragment length
   5% of sequence in fragments shorter than     304 bp
  10% of sequence in fragments shorter than     510 bp
  15% of sequence in fragments shorter than     696 bp
  20% of sequence in fragments shorter than     884 bp
  25% of sequence in fragments shorter than    1073 bp
  30% of sequence in fragments shorter than    1264 bp
  35% of sequence in fragments shorter than    1463 bp
  40% of sequence in fragments shorter than    1667 bp
  45% of sequence in fragments shorter than    1889 bp
  50% of sequence in fragments shorter than    2127 bp
  55% of sequence in fragments shorter than    2386 bp
  60% of sequence in fragments shorter than    2666 bp
  65% of sequence in fragments shorter than    2975 bp
  70% of sequence in fragments shorter than    3332 bp
  75% of sequence in fragments shorter than    3750 bp
  80% of sequence in fragments shorter than    4264 bp
  85% of sequence in fragments shorter than    4926 bp
  90% of sequence in fragments shorter than    5865 bp
  95% of sequence in fragments shorter than    7537 bp

# NspI.PspXI (RCATGY+VCTCGAGB) double digest, 3163 thousand fragments (3104359+59193 sites), 942 bp average fragment length
   5% of sequence in fragments shorter than     329 bp
  10% of sequence in fragments shorter than     506 bp
  15% of sequence in fragments shorter than     662 bp
  20% of sequence in fragments shorter than     806 bp
  25% of sequence in fragments shorter than     947 bp
  30% of sequence in fragments shorter than    1088 bp
  35% of sequence in fragments shorter than    1230 bp
  40% of sequence in fragments shorter than    1379 bp
  45% of sequence in fragments shorter than    1534 bp
  50% of sequence in fragments shorter than    1696 bp
  55% of sequence in fragments shorter than    1872 bp
  60% of sequence in fragments shorter than    2064 bp
  65% of sequence in fragments shorter than    2271 bp
  70% of sequence in fragments shorter than    2514 bp
  75% of sequence in fragments shorter than    2792 bp
  80% of sequence in fragments shorter than    3129 bp
  85% of sequence in fragments shorter than    3556 bp
  90% of sequence in fragments shorter than    4169 bp
  95% of sequence in fragments shorter than    5354 bp

# AgeI.NspI (ACCGGT+RCATGY) double digest, 3157 thousand fragments (53386+3104359 sites), 943 bp average fragment length
   5% of sequence in fragments shorter than     329 bp
  10% of sequence in fragments shorter than     507 bp
  15% of sequence in fragments shorter than     664 bp
  20% of sequence in fragments shorter than     808 bp
  25% of sequence in fragments shorter than     951 bp
  30% of sequence in fragments shorter than    1092 bp
  35% of sequence in fragments shorter than    1234 bp
  40% of sequence in fragments shorter than    1384 bp
  45% of sequence in fragments shorter than    1539 bp
  50% of sequence in fragments shorter than    1702 bp
  55% of sequence in fragments shorter than    1879 bp
  60% of sequence in fragments shorter than    2071 bp
  65% of sequence in fragments shorter than    2278 bp
  70% of sequence in fragments shorter than    2521 bp
  75% of sequence in fragments shorter than    2798 bp
  80% of sequence in fragments shorter than    3134 bp
  85% of sequence in fragments shorter than    3558 bp
  90% of sequence in fragments shorter than    4144 bp
  95% of sequence in fragments shorter than    5298 bp

# PmeI.StyI (GTTTAAAC+CCWWGG) double digest, 3155 thousand fragments (42094+3114107 sites), 944 bp average fragment length
   5% of sequence in fragments shorter than     319 bp
  10% of sequence in fragments shorter than     490 bp
  15% of sequence in fragments shorter than     640 bp
  20% of sequence in fragments shorter than     773 bp
  25% of sequence in fragments shorter than     918 bp
  30% of sequence in fragments shorter than    1063 bp
  35% of sequence in fragments shorter than    1213 bp
  40% of sequence in fragments shorter than    1368 bp
  45% of sequence in fragments shorter than    1527 bp
  50% of sequence in fragments shorter than    1705 bp
  55% of sequence in fragments shorter than    1895 bp
  60% of sequence in fragments shorter than    2108 bp
  65% of sequence in fragments shorter than    2345 bp
  70% of sequence in fragments shorter than    2614 bp
  75% of sequence in fragments shorter than    2926 bp
  80% of sequence in fragments shorter than    3285 bp
  85% of sequence in fragments shorter than    3784 bp
  90% of sequence in fragments shorter than    4517 bp
  95% of sequence in fragments shorter than    5952 bp

# EsaBC3I.HgiCI (TCGA+GGYRCC) double digest, 3153 thousand fragments (1603346+1550877 sites), 944 bp average fragment length
   5% of sequence in fragments shorter than     317 bp
  10% of sequence in fragments shorter than     492 bp
  15% of sequence in fragments shorter than     652 bp
  20% of sequence in fragments shorter than     804 bp
  25% of sequence in fragments shorter than     954 bp
  30% of sequence in fragments shorter than    1107 bp
  35% of sequence in fragments shorter than    1261 bp
  40% of sequence in fragments shorter than    1419 bp
  45% of sequence in fragments shorter than    1593 bp
  50% of sequence in fragments shorter than    1782 bp
  55% of sequence in fragments shorter than    1987 bp
  60% of sequence in fragments shorter than    2215 bp
  65% of sequence in fragments shorter than    2468 bp
  70% of sequence in fragments shorter than    2755 bp
  75% of sequence in fragments shorter than    3085 bp
  80% of sequence in fragments shorter than    3498 bp
  85% of sequence in fragments shorter than    4027 bp
  90% of sequence in fragments shorter than    4784 bp
  95% of sequence in fragments shorter than    6103 bp

# BsmI.EsaBC3I (GAATGC+TCGA) double digest, 3152 thousand fragments (1549350+1603346 sites), 945 bp average fragment length
   5% of sequence in fragments shorter than     316 bp
  10% of sequence in fragments shorter than     509 bp
  15% of sequence in fragments shorter than     682 bp
  20% of sequence in fragments shorter than     842 bp
  25% of sequence in fragments shorter than     995 bp
  30% of sequence in fragments shorter than    1149 bp
  35% of sequence in fragments shorter than    1305 bp
  40% of sequence in fragments shorter than    1464 bp
  45% of sequence in fragments shorter than    1633 bp
  50% of sequence in fragments shorter than    1810 bp
  55% of sequence in fragments shorter than    2000 bp
  60% of sequence in fragments shorter than    2202 bp
  65% of sequence in fragments shorter than    2428 bp
  70% of sequence in fragments shorter than    2679 bp
  75% of sequence in fragments shorter than    2972 bp
  80% of sequence in fragments shorter than    3322 bp
  85% of sequence in fragments shorter than    3764 bp
  90% of sequence in fragments shorter than    4371 bp
  95% of sequence in fragments shorter than    5400 bp

# BglII.SspI (AGATCT+AATATT) double digest, 3151 thousand fragments (774732+2377267 sites), 945 bp average fragment length
   5% of sequence in fragments shorter than     313 bp
  10% of sequence in fragments shorter than     485 bp
  15% of sequence in fragments shorter than     641 bp
  20% of sequence in fragments shorter than     786 bp
  25% of sequence in fragments shorter than     935 bp
  30% of sequence in fragments shorter than    1083 bp
  35% of sequence in fragments shorter than    1238 bp
  40% of sequence in fragments shorter than    1399 bp
  45% of sequence in fragments shorter than    1572 bp
  50% of sequence in fragments shorter than    1763 bp
  55% of sequence in fragments shorter than    1974 bp
  60% of sequence in fragments shorter than    2207 bp
  65% of sequence in fragments shorter than    2477 bp
  70% of sequence in fragments shorter than    2795 bp
  75% of sequence in fragments shorter than    3185 bp
  80% of sequence in fragments shorter than    3668 bp
  85% of sequence in fragments shorter than    4317 bp
  90% of sequence in fragments shorter than    5350 bp
  95% of sequence in fragments shorter than    7650 bp

# AvaII.SpeI (GGWCC+ACTAGT) double digest, 3147 thousand fragments (2747690+400286 sites), 946 bp average fragment length
   5% of sequence in fragments shorter than     314 bp
  10% of sequence in fragments shorter than     490 bp
  15% of sequence in fragments shorter than     657 bp
  20% of sequence in fragments shorter than     816 bp
  25% of sequence in fragments shorter than     962 bp
  30% of sequence in fragments shorter than    1123 bp
  35% of sequence in fragments shorter than    1283 bp
  40% of sequence in fragments shorter than    1456 bp
  45% of sequence in fragments shorter than    1642 bp
  50% of sequence in fragments shorter than    1833 bp
  55% of sequence in fragments shorter than    2044 bp
  60% of sequence in fragments shorter than    2270 bp
  65% of sequence in fragments shorter than    2531 bp
  70% of sequence in fragments shorter than    2816 bp
  75% of sequence in fragments shorter than    3159 bp
  80% of sequence in fragments shorter than    3578 bp
  85% of sequence in fragments shorter than    4122 bp
  90% of sequence in fragments shorter than    4898 bp
  95% of sequence in fragments shorter than    6294 bp

# NspI.PmeI (RCATGY+GTTTAAAC) double digest, 3145 thousand fragments (3104359+42094 sites), 947 bp average fragment length
   5% of sequence in fragments shorter than     330 bp
  10% of sequence in fragments shorter than     508 bp
  15% of sequence in fragments shorter than     666 bp
  20% of sequence in fragments shorter than     810 bp
  25% of sequence in fragments shorter than     953 bp
  30% of sequence in fragments shorter than    1095 bp
  35% of sequence in fragments shorter than    1238 bp
  40% of sequence in fragments shorter than    1388 bp
  45% of sequence in fragments shorter than    1545 bp
  50% of sequence in fragments shorter than    1709 bp
  55% of sequence in fragments shorter than    1886 bp
  60% of sequence in fragments shorter than    2080 bp
  65% of sequence in fragments shorter than    2290 bp
  70% of sequence in fragments shorter than    2536 bp
  75% of sequence in fragments shorter than    2818 bp
  80% of sequence in fragments shorter than    3161 bp
  85% of sequence in fragments shorter than    3592 bp
  90% of sequence in fragments shorter than    4218 bp
  95% of sequence in fragments shorter than    5441 bp

# BfiI.FauI (ACTGGG+CCCGC) double digest, 3145 thousand fragments (1706594+1439772 sites), 947 bp average fragment length
   5% of sequence in fragments shorter than     315 bp
  10% of sequence in fragments shorter than     511 bp
  15% of sequence in fragments shorter than     694 bp
  20% of sequence in fragments shorter than     872 bp
  25% of sequence in fragments shorter than    1039 bp
  30% of sequence in fragments shorter than    1223 bp
  35% of sequence in fragments shorter than    1406 bp
  40% of sequence in fragments shorter than    1607 bp
  45% of sequence in fragments shorter than    1809 bp
  50% of sequence in fragments shorter than    2035 bp
  55% of sequence in fragments shorter than    2266 bp
  60% of sequence in fragments shorter than    2538 bp
  65% of sequence in fragments shorter than    2826 bp
  70% of sequence in fragments shorter than    3178 bp
  75% of sequence in fragments shorter than    3589 bp
  80% of sequence in fragments shorter than    4092 bp
  85% of sequence in fragments shorter than    4750 bp
  90% of sequence in fragments shorter than    5674 bp
  95% of sequence in fragments shorter than    7464 bp

# SalI.StyI (GTCGAC+CCWWGG) double digest, 3145 thousand fragments (31396+3114107 sites), 947 bp average fragment length
   5% of sequence in fragments shorter than     319 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     642 bp
  20% of sequence in fragments shorter than     776 bp
  25% of sequence in fragments shorter than     922 bp
  30% of sequence in fragments shorter than    1068 bp
  35% of sequence in fragments shorter than    1219 bp
  40% of sequence in fragments shorter than    1375 bp
  45% of sequence in fragments shorter than    1537 bp
  50% of sequence in fragments shorter than    1716 bp
  55% of sequence in fragments shorter than    1909 bp
  60% of sequence in fragments shorter than    2125 bp
  65% of sequence in fragments shorter than    2365 bp
  70% of sequence in fragments shorter than    2637 bp
  75% of sequence in fragments shorter than    2953 bp
  80% of sequence in fragments shorter than    3313 bp
  85% of sequence in fragments shorter than    3824 bp
  90% of sequence in fragments shorter than    4570 bp
  95% of sequence in fragments shorter than    6049 bp

# BfiI.BspMI (ACTGGG+ACCTGC) double digest, 3144 thousand fragments (1706594+1438129 sites), 947 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     482 bp
  15% of sequence in fragments shorter than     644 bp
  20% of sequence in fragments shorter than     793 bp
  25% of sequence in fragments shorter than     939 bp
  30% of sequence in fragments shorter than    1090 bp
  35% of sequence in fragments shorter than    1249 bp
  40% of sequence in fragments shorter than    1407 bp
  45% of sequence in fragments shorter than    1581 bp
  50% of sequence in fragments shorter than    1765 bp
  55% of sequence in fragments shorter than    1962 bp
  60% of sequence in fragments shorter than    2166 bp
  65% of sequence in fragments shorter than    2409 bp
  70% of sequence in fragments shorter than    2689 bp
  75% of sequence in fragments shorter than    2969 bp
  80% of sequence in fragments shorter than    3345 bp
  85% of sequence in fragments shorter than    3839 bp
  90% of sequence in fragments shorter than    4534 bp
  95% of sequence in fragments shorter than    5733 bp

# AvaII.HpaI (GGWCC+GTTAAC) double digest, 3136 thousand fragments (2747690+389418 sites), 950 bp average fragment length
   5% of sequence in fragments shorter than     314 bp
  10% of sequence in fragments shorter than     489 bp
  15% of sequence in fragments shorter than     657 bp
  20% of sequence in fragments shorter than     816 bp
  25% of sequence in fragments shorter than     962 bp
  30% of sequence in fragments shorter than    1128 bp
  35% of sequence in fragments shorter than    1299 bp
  40% of sequence in fragments shorter than    1476 bp
  45% of sequence in fragments shorter than    1665 bp
  50% of sequence in fragments shorter than    1856 bp
  55% of sequence in fragments shorter than    2070 bp
  60% of sequence in fragments shorter than    2306 bp
  65% of sequence in fragments shorter than    2572 bp
  70% of sequence in fragments shorter than    2870 bp
  75% of sequence in fragments shorter than    3224 bp
  80% of sequence in fragments shorter than    3654 bp
  85% of sequence in fragments shorter than    4213 bp
  90% of sequence in fragments shorter than    5017 bp
  95% of sequence in fragments shorter than    6499 bp

# NcoI.SspI (CCATGG+AATATT) double digest, 3136 thousand fragments (759594+2377267 sites), 950 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     498 bp
  15% of sequence in fragments shorter than     649 bp
  20% of sequence in fragments shorter than     794 bp
  25% of sequence in fragments shorter than     938 bp
  30% of sequence in fragments shorter than    1084 bp
  35% of sequence in fragments shorter than    1232 bp
  40% of sequence in fragments shorter than    1383 bp
  45% of sequence in fragments shorter than    1544 bp
  50% of sequence in fragments shorter than    1719 bp
  55% of sequence in fragments shorter than    1909 bp
  60% of sequence in fragments shorter than    2117 bp
  65% of sequence in fragments shorter than    2356 bp
  70% of sequence in fragments shorter than    2626 bp
  75% of sequence in fragments shorter than    2954 bp
  80% of sequence in fragments shorter than    3349 bp
  85% of sequence in fragments shorter than    3835 bp
  90% of sequence in fragments shorter than    4590 bp
  95% of sequence in fragments shorter than    6093 bp

# NspI.SalI (RCATGY+GTCGAC) double digest, 3135 thousand fragments (3104359+31396 sites), 950 bp average fragment length
   5% of sequence in fragments shorter than     331 bp
  10% of sequence in fragments shorter than     510 bp
  15% of sequence in fragments shorter than     668 bp
  20% of sequence in fragments shorter than     814 bp
  25% of sequence in fragments shorter than     957 bp
  30% of sequence in fragments shorter than    1099 bp
  35% of sequence in fragments shorter than    1242 bp
  40% of sequence in fragments shorter than    1393 bp
  45% of sequence in fragments shorter than    1550 bp
  50% of sequence in fragments shorter than    1714 bp
  55% of sequence in fragments shorter than    1892 bp
  60% of sequence in fragments shorter than    2085 bp
  65% of sequence in fragments shorter than    2296 bp
  70% of sequence in fragments shorter than    2542 bp
  75% of sequence in fragments shorter than    2823 bp
  80% of sequence in fragments shorter than    3166 bp
  85% of sequence in fragments shorter than    3597 bp
  90% of sequence in fragments shorter than    4222 bp
  95% of sequence in fragments shorter than    5437 bp

# MluI.StyI (ACGCGT+CCWWGG) double digest, 3135 thousand fragments (21415+3114107 sites), 950 bp average fragment length
   5% of sequence in fragments shorter than     320 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     643 bp
  20% of sequence in fragments shorter than     778 bp
  25% of sequence in fragments shorter than     925 bp
  30% of sequence in fragments shorter than    1072 bp
  35% of sequence in fragments shorter than    1224 bp
  40% of sequence in fragments shorter than    1380 bp
  45% of sequence in fragments shorter than    1543 bp
  50% of sequence in fragments shorter than    1722 bp
  55% of sequence in fragments shorter than    1916 bp
  60% of sequence in fragments shorter than    2133 bp
  65% of sequence in fragments shorter than    2375 bp
  70% of sequence in fragments shorter than    2649 bp
  75% of sequence in fragments shorter than    2968 bp
  80% of sequence in fragments shorter than    3328 bp
  85% of sequence in fragments shorter than    3842 bp
  90% of sequence in fragments shorter than    4593 bp
  95% of sequence in fragments shorter than    6089 bp

# AflIII.PsiI (ACRYGT+TTATAA) double digest, 3132 thousand fragments (1485394+1647912 sites), 951 bp average fragment length
   5% of sequence in fragments shorter than     324 bp
  10% of sequence in fragments shorter than     503 bp
  15% of sequence in fragments shorter than     667 bp
  20% of sequence in fragments shorter than     823 bp
  25% of sequence in fragments shorter than     976 bp
  30% of sequence in fragments shorter than    1132 bp
  35% of sequence in fragments shorter than    1293 bp
  40% of sequence in fragments shorter than    1457 bp
  45% of sequence in fragments shorter than    1635 bp
  50% of sequence in fragments shorter than    1822 bp
  55% of sequence in fragments shorter than    2027 bp
  60% of sequence in fragments shorter than    2249 bp
  65% of sequence in fragments shorter than    2505 bp
  70% of sequence in fragments shorter than    2792 bp
  75% of sequence in fragments shorter than    3110 bp
  80% of sequence in fragments shorter than    3529 bp
  85% of sequence in fragments shorter than    4095 bp
  90% of sequence in fragments shorter than    4959 bp
  95% of sequence in fragments shorter than    6602 bp

# SgrAI.StyI (CRCCGGYG+CCWWGG) double digest, 3128 thousand fragments (15270+3114107 sites), 952 bp average fragment length
   5% of sequence in fragments shorter than     320 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     644 bp
  20% of sequence in fragments shorter than     780 bp
  25% of sequence in fragments shorter than     927 bp
  30% of sequence in fragments shorter than    1074 bp
  35% of sequence in fragments shorter than    1227 bp
  40% of sequence in fragments shorter than    1383 bp
  45% of sequence in fragments shorter than    1547 bp
  50% of sequence in fragments shorter than    1727 bp
  55% of sequence in fragments shorter than    1921 bp
  60% of sequence in fragments shorter than    2139 bp
  65% of sequence in fragments shorter than    2381 bp
  70% of sequence in fragments shorter than    2656 bp
  75% of sequence in fragments shorter than    2976 bp
  80% of sequence in fragments shorter than    3337 bp
  85% of sequence in fragments shorter than    3853 bp
  90% of sequence in fragments shorter than    4606 bp
  95% of sequence in fragments shorter than    6104 bp

# NruI.StyI (TCGCGA+CCWWGG) double digest, 3128 thousand fragments (14872+3114107 sites), 952 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     644 bp
  20% of sequence in fragments shorter than     780 bp
  25% of sequence in fragments shorter than     927 bp
  30% of sequence in fragments shorter than    1074 bp
  35% of sequence in fragments shorter than    1226 bp
  40% of sequence in fragments shorter than    1382 bp
  45% of sequence in fragments shorter than    1546 bp
  50% of sequence in fragments shorter than    1725 bp
  55% of sequence in fragments shorter than    1919 bp
  60% of sequence in fragments shorter than    2137 bp
  65% of sequence in fragments shorter than    2378 bp
  70% of sequence in fragments shorter than    2653 bp
  75% of sequence in fragments shorter than    2971 bp
  80% of sequence in fragments shorter than    3331 bp
  85% of sequence in fragments shorter than    3846 bp
  90% of sequence in fragments shorter than    4596 bp
  95% of sequence in fragments shorter than    6087 bp

# PvuI.StyI (CGATCG+CCWWGG) double digest, 3127 thousand fragments (14186+3114107 sites), 952 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     645 bp
  20% of sequence in fragments shorter than     781 bp
  25% of sequence in fragments shorter than     928 bp
  30% of sequence in fragments shorter than    1075 bp
  35% of sequence in fragments shorter than    1227 bp
  40% of sequence in fragments shorter than    1383 bp
  45% of sequence in fragments shorter than    1547 bp
  50% of sequence in fragments shorter than    1726 bp
  55% of sequence in fragments shorter than    1918 bp
  60% of sequence in fragments shorter than    2135 bp
  65% of sequence in fragments shorter than    2376 bp
  70% of sequence in fragments shorter than    2651 bp
  75% of sequence in fragments shorter than    2969 bp
  80% of sequence in fragments shorter than    3328 bp
  85% of sequence in fragments shorter than    3839 bp
  90% of sequence in fragments shorter than    4585 bp
  95% of sequence in fragments shorter than    6046 bp

# SmlI.SpeI (CTYRAG+ACTAGT) double digest, 3127 thousand fragments (2727867+400286 sites), 952 bp average fragment length
   5% of sequence in fragments shorter than     327 bp
  10% of sequence in fragments shorter than     502 bp
  15% of sequence in fragments shorter than     648 bp
  20% of sequence in fragments shorter than     786 bp
  25% of sequence in fragments shorter than     922 bp
  30% of sequence in fragments shorter than    1054 bp
  35% of sequence in fragments shorter than    1187 bp
  40% of sequence in fragments shorter than    1324 bp
  45% of sequence in fragments shorter than    1471 bp
  50% of sequence in fragments shorter than    1630 bp
  55% of sequence in fragments shorter than    1799 bp
  60% of sequence in fragments shorter than    1981 bp
  65% of sequence in fragments shorter than    2180 bp
  70% of sequence in fragments shorter than    2413 bp
  75% of sequence in fragments shorter than    2657 bp
  80% of sequence in fragments shorter than    2974 bp
  85% of sequence in fragments shorter than    3391 bp
  90% of sequence in fragments shorter than    3976 bp
  95% of sequence in fragments shorter than    5076 bp

# FseI.StyI (GGCCGGCC+CCWWGG) double digest, 3127 thousand fragments (13662+3114107 sites), 952 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     645 bp
  20% of sequence in fragments shorter than     781 bp
  25% of sequence in fragments shorter than     928 bp
  30% of sequence in fragments shorter than    1075 bp
  35% of sequence in fragments shorter than    1228 bp
  40% of sequence in fragments shorter than    1383 bp
  45% of sequence in fragments shorter than    1548 bp
  50% of sequence in fragments shorter than    1728 bp
  55% of sequence in fragments shorter than    1923 bp
  60% of sequence in fragments shorter than    2141 bp
  65% of sequence in fragments shorter than    2383 bp
  70% of sequence in fragments shorter than    2659 bp
  75% of sequence in fragments shorter than    2978 bp
  80% of sequence in fragments shorter than    3340 bp
  85% of sequence in fragments shorter than    3856 bp
  90% of sequence in fragments shorter than    4609 bp
  95% of sequence in fragments shorter than    6108 bp

# MluI.NspI (ACGCGT+RCATGY) double digest, 3125 thousand fragments (21415+3104359 sites), 953 bp average fragment length
   5% of sequence in fragments shorter than     332 bp
  10% of sequence in fragments shorter than     512 bp
  15% of sequence in fragments shorter than     671 bp
  20% of sequence in fragments shorter than     817 bp
  25% of sequence in fragments shorter than     961 bp
  30% of sequence in fragments shorter than    1103 bp
  35% of sequence in fragments shorter than    1247 bp
  40% of sequence in fragments shorter than    1399 bp
  45% of sequence in fragments shorter than    1556 bp
  50% of sequence in fragments shorter than    1721 bp
  55% of sequence in fragments shorter than    1899 bp
  60% of sequence in fragments shorter than    2089 bp
  65% of sequence in fragments shorter than    2304 bp
  70% of sequence in fragments shorter than    2552 bp
  75% of sequence in fragments shorter than    2834 bp
  80% of sequence in fragments shorter than    3177 bp
  85% of sequence in fragments shorter than    3610 bp
  90% of sequence in fragments shorter than    4238 bp
  95% of sequence in fragments shorter than    5460 bp

# AvaII.SapI (GGWCC+GCTCTTC) double digest, 3124 thousand fragments (2747690+377161 sites), 953 bp average fragment length
   5% of sequence in fragments shorter than     308 bp
  10% of sequence in fragments shorter than     487 bp
  15% of sequence in fragments shorter than     661 bp
  20% of sequence in fragments shorter than     820 bp
  25% of sequence in fragments shorter than     975 bp
  30% of sequence in fragments shorter than    1147 bp
  35% of sequence in fragments shorter than    1329 bp
  40% of sequence in fragments shorter than    1519 bp
  45% of sequence in fragments shorter than    1721 bp
  50% of sequence in fragments shorter than    1935 bp
  55% of sequence in fragments shorter than    2168 bp
  60% of sequence in fragments shorter than    2428 bp
  65% of sequence in fragments shorter than    2719 bp
  70% of sequence in fragments shorter than    3052 bp
  75% of sequence in fragments shorter than    3441 bp
  80% of sequence in fragments shorter than    3924 bp
  85% of sequence in fragments shorter than    4552 bp
  90% of sequence in fragments shorter than    5454 bp
  95% of sequence in fragments shorter than    7130 bp

# AvaII.SmaI (GGWCC+CCCGGG) double digest, 3124 thousand fragments (2747690+376940 sites), 953 bp average fragment length
   5% of sequence in fragments shorter than     310 bp
  10% of sequence in fragments shorter than     487 bp
  15% of sequence in fragments shorter than     662 bp
  20% of sequence in fragments shorter than     830 bp
  25% of sequence in fragments shorter than     987 bp
  30% of sequence in fragments shorter than    1166 bp
  35% of sequence in fragments shorter than    1351 bp
  40% of sequence in fragments shorter than    1543 bp
  45% of sequence in fragments shorter than    1747 bp
  50% of sequence in fragments shorter than    1963 bp
  55% of sequence in fragments shorter than    2205 bp
  60% of sequence in fragments shorter than    2470 bp
  65% of sequence in fragments shorter than    2758 bp
  70% of sequence in fragments shorter than    3100 bp
  75% of sequence in fragments shorter than    3501 bp
  80% of sequence in fragments shorter than    3998 bp
  85% of sequence in fragments shorter than    4647 bp
  90% of sequence in fragments shorter than    5587 bp
  95% of sequence in fragments shorter than    7345 bp

# NotI.StyI (GCGGCCGC+CCWWGG) double digest, 3123 thousand fragments (9795+3114107 sites), 954 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     492 bp
  15% of sequence in fragments shorter than     645 bp
  20% of sequence in fragments shorter than     781 bp
  25% of sequence in fragments shorter than     929 bp
  30% of sequence in fragments shorter than    1076 bp
  35% of sequence in fragments shorter than    1228 bp
  40% of sequence in fragments shorter than    1384 bp
  45% of sequence in fragments shorter than    1549 bp
  50% of sequence in fragments shorter than    1729 bp
  55% of sequence in fragments shorter than    1924 bp
  60% of sequence in fragments shorter than    2142 bp
  65% of sequence in fragments shorter than    2384 bp
  70% of sequence in fragments shorter than    2660 bp
  75% of sequence in fragments shorter than    2979 bp
  80% of sequence in fragments shorter than    3341 bp
  85% of sequence in fragments shorter than    3857 bp
  90% of sequence in fragments shorter than    4612 bp
  95% of sequence in fragments shorter than    6112 bp

# RsrII.StyI (CGGWCCG+CCWWGG) double digest, 3123 thousand fragments (9764+3114107 sites), 954 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     492 bp
  15% of sequence in fragments shorter than     645 bp
  20% of sequence in fragments shorter than     781 bp
  25% of sequence in fragments shorter than     929 bp
  30% of sequence in fragments shorter than    1076 bp
  35% of sequence in fragments shorter than    1228 bp
  40% of sequence in fragments shorter than    1384 bp
  45% of sequence in fragments shorter than    1549 bp
  50% of sequence in fragments shorter than    1729 bp
  55% of sequence in fragments shorter than    1924 bp
  60% of sequence in fragments shorter than    2141 bp
  65% of sequence in fragments shorter than    2383 bp
  70% of sequence in fragments shorter than    2659 bp
  75% of sequence in fragments shorter than    2978 bp
  80% of sequence in fragments shorter than    3339 bp
  85% of sequence in fragments shorter than    3856 bp
  90% of sequence in fragments shorter than    4610 bp
  95% of sequence in fragments shorter than    6107 bp

# NspI.SgrAI (RCATGY+CRCCGGYG) double digest, 3119 thousand fragments (3104359+15270 sites), 955 bp average fragment length
   5% of sequence in fragments shorter than     333 bp
  10% of sequence in fragments shorter than     513 bp
  15% of sequence in fragments shorter than     672 bp
  20% of sequence in fragments shorter than     818 bp
  25% of sequence in fragments shorter than     962 bp
  30% of sequence in fragments shorter than    1105 bp
  35% of sequence in fragments shorter than    1249 bp
  40% of sequence in fragments shorter than    1400 bp
  45% of sequence in fragments shorter than    1558 bp
  50% of sequence in fragments shorter than    1723 bp
  55% of sequence in fragments shorter than    1901 bp
  60% of sequence in fragments shorter than    2091 bp
  65% of sequence in fragments shorter than    2306 bp
  70% of sequence in fragments shorter than    2554 bp
  75% of sequence in fragments shorter than    2835 bp
  80% of sequence in fragments shorter than    3179 bp
  85% of sequence in fragments shorter than    3611 bp
  90% of sequence in fragments shorter than    4239 bp
  95% of sequence in fragments shorter than    5458 bp

# NruI.NspI (TCGCGA+RCATGY) double digest, 3118 thousand fragments (14872+3104359 sites), 955 bp average fragment length
   5% of sequence in fragments shorter than     333 bp
  10% of sequence in fragments shorter than     513 bp
  15% of sequence in fragments shorter than     672 bp
  20% of sequence in fragments shorter than     818 bp
  25% of sequence in fragments shorter than     962 bp
  30% of sequence in fragments shorter than    1105 bp
  35% of sequence in fragments shorter than    1249 bp
  40% of sequence in fragments shorter than    1401 bp
  45% of sequence in fragments shorter than    1558 bp
  50% of sequence in fragments shorter than    1724 bp
  55% of sequence in fragments shorter than    1902 bp
  60% of sequence in fragments shorter than    2092 bp
  65% of sequence in fragments shorter than    2308 bp
  70% of sequence in fragments shorter than    2556 bp
  75% of sequence in fragments shorter than    2837 bp
  80% of sequence in fragments shorter than    3182 bp
  85% of sequence in fragments shorter than    3615 bp
  90% of sequence in fragments shorter than    4243 bp
  95% of sequence in fragments shorter than    5465 bp

# AscI.StyI (GGCGCGCC+CCWWGG) double digest, 3118 thousand fragments (4751+3114107 sites), 955 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     492 bp
  15% of sequence in fragments shorter than     646 bp
  20% of sequence in fragments shorter than     783 bp
  25% of sequence in fragments shorter than     930 bp
  30% of sequence in fragments shorter than    1077 bp
  35% of sequence in fragments shorter than    1230 bp
  40% of sequence in fragments shorter than    1385 bp
  45% of sequence in fragments shorter than    1551 bp
  50% of sequence in fragments shorter than    1731 bp
  55% of sequence in fragments shorter than    1926 bp
  60% of sequence in fragments shorter than    2144 bp
  65% of sequence in fragments shorter than    2386 bp
  70% of sequence in fragments shorter than    2662 bp
  75% of sequence in fragments shorter than    2982 bp
  80% of sequence in fragments shorter than    3343 bp
  85% of sequence in fragments shorter than    3860 bp
  90% of sequence in fragments shorter than    4614 bp
  95% of sequence in fragments shorter than    6113 bp

# NspI.PvuI (RCATGY+CGATCG) double digest, 3118 thousand fragments (3104359+14186 sites), 955 bp average fragment length
   5% of sequence in fragments shorter than     333 bp
  10% of sequence in fragments shorter than     514 bp
  15% of sequence in fragments shorter than     672 bp
  20% of sequence in fragments shorter than     819 bp
  25% of sequence in fragments shorter than     963 bp
  30% of sequence in fragments shorter than    1105 bp
  35% of sequence in fragments shorter than    1250 bp
  40% of sequence in fragments shorter than    1402 bp
  45% of sequence in fragments shorter than    1560 bp
  50% of sequence in fragments shorter than    1725 bp
  55% of sequence in fragments shorter than    1901 bp
  60% of sequence in fragments shorter than    2092 bp
  65% of sequence in fragments shorter than    2307 bp
  70% of sequence in fragments shorter than    2556 bp
  75% of sequence in fragments shorter than    2836 bp
  80% of sequence in fragments shorter than    3180 bp
  85% of sequence in fragments shorter than    3612 bp
  90% of sequence in fragments shorter than    4238 bp
  95% of sequence in fragments shorter than    5440 bp

# FseI.NspI (GGCCGGCC+RCATGY) double digest, 3117 thousand fragments (13662+3104359 sites), 955 bp average fragment length
   5% of sequence in fragments shorter than     333 bp
  10% of sequence in fragments shorter than     514 bp
  15% of sequence in fragments shorter than     672 bp
  20% of sequence in fragments shorter than     819 bp
  25% of sequence in fragments shorter than     963 bp
  30% of sequence in fragments shorter than    1105 bp
  35% of sequence in fragments shorter than    1250 bp
  40% of sequence in fragments shorter than    1401 bp
  45% of sequence in fragments shorter than    1559 bp
  50% of sequence in fragments shorter than    1724 bp
  55% of sequence in fragments shorter than    1902 bp
  60% of sequence in fragments shorter than    2092 bp
  65% of sequence in fragments shorter than    2307 bp
  70% of sequence in fragments shorter than    2556 bp
  75% of sequence in fragments shorter than    2837 bp
  80% of sequence in fragments shorter than    3181 bp
  85% of sequence in fragments shorter than    3615 bp
  90% of sequence in fragments shorter than    4243 bp
  95% of sequence in fragments shorter than    5463 bp

# HpaI.SmlI (GTTAAC+CTYRAG) double digest, 3116 thousand fragments (389418+2727867 sites), 956 bp average fragment length
   5% of sequence in fragments shorter than     328 bp
  10% of sequence in fragments shorter than     503 bp
  15% of sequence in fragments shorter than     649 bp
  20% of sequence in fragments shorter than     788 bp
  25% of sequence in fragments shorter than     925 bp
  30% of sequence in fragments shorter than    1055 bp
  35% of sequence in fragments shorter than    1194 bp
  40% of sequence in fragments shorter than    1338 bp
  45% of sequence in fragments shorter than    1485 bp
  50% of sequence in fragments shorter than    1647 bp
  55% of sequence in fragments shorter than    1811 bp
  60% of sequence in fragments shorter than    1996 bp
  65% of sequence in fragments shorter than    2195 bp
  70% of sequence in fragments shorter than    2426 bp
  75% of sequence in fragments shorter than    2684 bp
  80% of sequence in fragments shorter than    3007 bp
  85% of sequence in fragments shorter than    3426 bp
  90% of sequence in fragments shorter than    4025 bp
  95% of sequence in fragments shorter than    5163 bp

# BclI.SspI (TGATCA+AATATT) double digest, 3115 thousand fragments (738785+2377267 sites), 956 bp average fragment length
   5% of sequence in fragments shorter than     316 bp
  10% of sequence in fragments shorter than     495 bp
  15% of sequence in fragments shorter than     654 bp
  20% of sequence in fragments shorter than     801 bp
  25% of sequence in fragments shorter than     949 bp
  30% of sequence in fragments shorter than    1103 bp
  35% of sequence in fragments shorter than    1264 bp
  40% of sequence in fragments shorter than    1428 bp
  45% of sequence in fragments shorter than    1606 bp
  50% of sequence in fragments shorter than    1791 bp
  55% of sequence in fragments shorter than    2005 bp
  60% of sequence in fragments shorter than    2247 bp
  65% of sequence in fragments shorter than    2521 bp
  70% of sequence in fragments shorter than    2839 bp
  75% of sequence in fragments shorter than    3223 bp
  80% of sequence in fragments shorter than    3715 bp
  85% of sequence in fragments shorter than    4391 bp
  90% of sequence in fragments shorter than    5463 bp
  95% of sequence in fragments shorter than    7902 bp

# NotI.NspI (GCGGCCGC+RCATGY) double digest, 3113 thousand fragments (9795+3104359 sites), 957 bp average fragment length
   5% of sequence in fragments shorter than     334 bp
  10% of sequence in fragments shorter than     514 bp
  15% of sequence in fragments shorter than     673 bp
  20% of sequence in fragments shorter than     820 bp
  25% of sequence in fragments shorter than     964 bp
  30% of sequence in fragments shorter than    1107 bp
  35% of sequence in fragments shorter than    1251 bp
  40% of sequence in fragments shorter than    1403 bp
  45% of sequence in fragments shorter than    1561 bp
  50% of sequence in fragments shorter than    1726 bp
  55% of sequence in fragments shorter than    1904 bp
  60% of sequence in fragments shorter than    2094 bp
  65% of sequence in fragments shorter than    2310 bp
  70% of sequence in fragments shorter than    2559 bp
  75% of sequence in fragments shorter than    2840 bp
  80% of sequence in fragments shorter than    3184 bp
  85% of sequence in fragments shorter than    3617 bp
  90% of sequence in fragments shorter than    4247 bp
  95% of sequence in fragments shorter than    5468 bp

# NspI.RsrII (RCATGY+CGGWCCG) double digest, 3113 thousand fragments (3104359+9764 sites), 957 bp average fragment length
   5% of sequence in fragments shorter than     334 bp
  10% of sequence in fragments shorter than     514 bp
  15% of sequence in fragments shorter than     673 bp
  20% of sequence in fragments shorter than     820 bp
  25% of sequence in fragments shorter than     964 bp
  30% of sequence in fragments shorter than    1107 bp
  35% of sequence in fragments shorter than    1251 bp
  40% of sequence in fragments shorter than    1403 bp
  45% of sequence in fragments shorter than    1561 bp
  50% of sequence in fragments shorter than    1726 bp
  55% of sequence in fragments shorter than    1904 bp
  60% of sequence in fragments shorter than    2095 bp
  65% of sequence in fragments shorter than    2311 bp
  70% of sequence in fragments shorter than    2560 bp
  75% of sequence in fragments shorter than    2841 bp
  80% of sequence in fragments shorter than    3186 bp
  85% of sequence in fragments shorter than    3619 bp
  90% of sequence in fragments shorter than    4247 bp
  95% of sequence in fragments shorter than    5466 bp

# NcoI.StyI (CCATGG+CCWWGG) double digest, 3113 thousand fragments (759594+3114107 sites), 957 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     493 bp
  15% of sequence in fragments shorter than     647 bp
  20% of sequence in fragments shorter than     784 bp
  25% of sequence in fragments shorter than     932 bp
  30% of sequence in fragments shorter than    1079 bp
  35% of sequence in fragments shorter than    1232 bp
  40% of sequence in fragments shorter than    1386 bp
  45% of sequence in fragments shorter than    1553 bp
  50% of sequence in fragments shorter than    1733 bp
  55% of sequence in fragments shorter than    1928 bp
  60% of sequence in fragments shorter than    2146 bp
  65% of sequence in fragments shorter than    2389 bp
  70% of sequence in fragments shorter than    2665 bp
  75% of sequence in fragments shorter than    2985 bp
  80% of sequence in fragments shorter than    3347 bp
  85% of sequence in fragments shorter than    3863 bp
  90% of sequence in fragments shorter than    4618 bp
  95% of sequence in fragments shorter than    6118 bp

# AvrII.StyI (CCTAGG+CCWWGG) double digest, 3113 thousand fragments (594956+3114107 sites), 957 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     493 bp
  15% of sequence in fragments shorter than     647 bp
  20% of sequence in fragments shorter than     784 bp
  25% of sequence in fragments shorter than     932 bp
  30% of sequence in fragments shorter than    1079 bp
  35% of sequence in fragments shorter than    1232 bp
  40% of sequence in fragments shorter than    1386 bp
  45% of sequence in fragments shorter than    1553 bp
  50% of sequence in fragments shorter than    1733 bp
  55% of sequence in fragments shorter than    1928 bp
  60% of sequence in fragments shorter than    2146 bp
  65% of sequence in fragments shorter than    2389 bp
  70% of sequence in fragments shorter than    2665 bp
  75% of sequence in fragments shorter than    2985 bp
  80% of sequence in fragments shorter than    3347 bp
  85% of sequence in fragments shorter than    3863 bp
  90% of sequence in fragments shorter than    4618 bp
  95% of sequence in fragments shorter than    6118 bp

# AvaII.BamHI (GGWCC+GGATCC) double digest, 3113 thousand fragments (2747690+365999 sites), 957 bp average fragment length
   5% of sequence in fragments shorter than     310 bp
  10% of sequence in fragments shorter than     491 bp
  15% of sequence in fragments shorter than     660 bp
  20% of sequence in fragments shorter than     830 bp
  25% of sequence in fragments shorter than     988 bp
  30% of sequence in fragments shorter than    1166 bp
  35% of sequence in fragments shorter than    1350 bp
  40% of sequence in fragments shorter than    1537 bp
  45% of sequence in fragments shorter than    1738 bp
  50% of sequence in fragments shorter than    1953 bp
  55% of sequence in fragments shorter than    2191 bp
  60% of sequence in fragments shorter than    2452 bp
  65% of sequence in fragments shorter than    2737 bp
  70% of sequence in fragments shorter than    3071 bp
  75% of sequence in fragments shorter than    3466 bp
  80% of sequence in fragments shorter than    3957 bp
  85% of sequence in fragments shorter than    4589 bp
  90% of sequence in fragments shorter than    5509 bp
  95% of sequence in fragments shorter than    7205 bp

# FnuDII.SspI (CGCG+AATATT) double digest, 3110 thousand fragments (733940+2377267 sites), 957 bp average fragment length
   5% of sequence in fragments shorter than     324 bp
  10% of sequence in fragments shorter than     521 bp
  15% of sequence in fragments shorter than     693 bp
  20% of sequence in fragments shorter than     859 bp
  25% of sequence in fragments shorter than    1024 bp
  30% of sequence in fragments shorter than    1190 bp
  35% of sequence in fragments shorter than    1362 bp
  40% of sequence in fragments shorter than    1534 bp
  45% of sequence in fragments shorter than    1723 bp
  50% of sequence in fragments shorter than    1927 bp
  55% of sequence in fragments shorter than    2148 bp
  60% of sequence in fragments shorter than    2395 bp
  65% of sequence in fragments shorter than    2672 bp
  70% of sequence in fragments shorter than    2989 bp
  75% of sequence in fragments shorter than    3367 bp
  80% of sequence in fragments shorter than    3831 bp
  85% of sequence in fragments shorter than    4420 bp
  90% of sequence in fragments shorter than    5269 bp
  95% of sequence in fragments shorter than    6911 bp

# AscI.NspI (GGCGCGCC+RCATGY) double digest, 3108 thousand fragments (4751+3104359 sites), 958 bp average fragment length
   5% of sequence in fragments shorter than     334 bp
  10% of sequence in fragments shorter than     515 bp
  15% of sequence in fragments shorter than     674 bp
  20% of sequence in fragments shorter than     821 bp
  25% of sequence in fragments shorter than     966 bp
  30% of sequence in fragments shorter than    1109 bp
  35% of sequence in fragments shorter than    1254 bp
  40% of sequence in fragments shorter than    1405 bp
  45% of sequence in fragments shorter than    1564 bp
  50% of sequence in fragments shorter than    1730 bp
  55% of sequence in fragments shorter than    1908 bp
  60% of sequence in fragments shorter than    2100 bp
  65% of sequence in fragments shorter than    2316 bp
  70% of sequence in fragments shorter than    2566 bp
  75% of sequence in fragments shorter than    2848 bp
  80% of sequence in fragments shorter than    3194 bp
  85% of sequence in fragments shorter than    3630 bp
  90% of sequence in fragments shorter than    4263 bp
  95% of sequence in fragments shorter than    5488 bp

# SapI.SmlI (GCTCTTC+CTYRAG) double digest, 3104 thousand fragments (377161+2727867 sites), 959 bp average fragment length
   5% of sequence in fragments shorter than     330 bp
  10% of sequence in fragments shorter than     505 bp
  15% of sequence in fragments shorter than     653 bp
  20% of sequence in fragments shorter than     789 bp
  25% of sequence in fragments shorter than     928 bp
  30% of sequence in fragments shorter than    1063 bp
  35% of sequence in fragments shorter than    1203 bp
  40% of sequence in fragments shorter than    1348 bp
  45% of sequence in fragments shorter than    1499 bp
  50% of sequence in fragments shorter than    1664 bp
  55% of sequence in fragments shorter than    1838 bp
  60% of sequence in fragments shorter than    2013 bp
  65% of sequence in fragments shorter than    2216 bp
  70% of sequence in fragments shorter than    2451 bp
  75% of sequence in fragments shorter than    2720 bp
  80% of sequence in fragments shorter than    3051 bp
  85% of sequence in fragments shorter than    3480 bp
  90% of sequence in fragments shorter than    4100 bp
  95% of sequence in fragments shorter than    5263 bp

# SmaI.SmlI (CCCGGG+CTYRAG) double digest, 3104 thousand fragments (376940+2727867 sites), 959 bp average fragment length
   5% of sequence in fragments shorter than     324 bp
  10% of sequence in fragments shorter than     499 bp
  15% of sequence in fragments shorter than     647 bp
  20% of sequence in fragments shorter than     788 bp
  25% of sequence in fragments shorter than     928 bp
  30% of sequence in fragments shorter than    1065 bp
  35% of sequence in fragments shorter than    1208 bp
  40% of sequence in fragments shorter than    1355 bp
  45% of sequence in fragments shorter than    1511 bp
  50% of sequence in fragments shorter than    1680 bp
  55% of sequence in fragments shorter than    1860 bp
  60% of sequence in fragments shorter than    2054 bp
  65% of sequence in fragments shorter than    2267 bp
  70% of sequence in fragments shorter than    2515 bp
  75% of sequence in fragments shorter than    2775 bp
  80% of sequence in fragments shorter than    3120 bp
  85% of sequence in fragments shorter than    3570 bp
  90% of sequence in fragments shorter than    4212 bp
  95% of sequence in fragments shorter than    5440 bp

# NspI.SphI (RCATGY+GCATGC) double digest, 3103 thousand fragments (3104359+550952 sites), 960 bp average fragment length
   5% of sequence in fragments shorter than     334 bp
  10% of sequence in fragments shorter than     516 bp
  15% of sequence in fragments shorter than     675 bp
  20% of sequence in fragments shorter than     822 bp
  25% of sequence in fragments shorter than     967 bp
  30% of sequence in fragments shorter than    1111 bp
  35% of sequence in fragments shorter than    1256 bp
  40% of sequence in fragments shorter than    1408 bp
  45% of sequence in fragments shorter than    1567 bp
  50% of sequence in fragments shorter than    1734 bp
  55% of sequence in fragments shorter than    1913 bp
  60% of sequence in fragments shorter than    2106 bp
  65% of sequence in fragments shorter than    2322 bp
  70% of sequence in fragments shorter than    2574 bp
  75% of sequence in fragments shorter than    2857 bp
  80% of sequence in fragments shorter than    3205 bp
  85% of sequence in fragments shorter than    3643 bp
  90% of sequence in fragments shorter than    4282 bp
  95% of sequence in fragments shorter than    5516 bp

# BsmI.HgiCI (GAATGC+GGYRCC) double digest, 3099 thousand fragments (1549350+1550877 sites), 961 bp average fragment length
   5% of sequence in fragments shorter than     309 bp
  10% of sequence in fragments shorter than     490 bp
  15% of sequence in fragments shorter than     663 bp
  20% of sequence in fragments shorter than     823 bp
  25% of sequence in fragments shorter than     983 bp
  30% of sequence in fragments shorter than    1144 bp
  35% of sequence in fragments shorter than    1308 bp
  40% of sequence in fragments shorter than    1479 bp
  45% of sequence in fragments shorter than    1652 bp
  50% of sequence in fragments shorter than    1839 bp
  55% of sequence in fragments shorter than    2040 bp
  60% of sequence in fragments shorter than    2256 bp
  65% of sequence in fragments shorter than    2499 bp
  70% of sequence in fragments shorter than    2772 bp
  75% of sequence in fragments shorter than    3086 bp
  80% of sequence in fragments shorter than    3469 bp
  85% of sequence in fragments shorter than    3955 bp
  90% of sequence in fragments shorter than    4632 bp
  95% of sequence in fragments shorter than    5779 bp

# BamHI.SmlI (GGATCC+CTYRAG) double digest, 3093 thousand fragments (365999+2727867 sites), 963 bp average fragment length
   5% of sequence in fragments shorter than     332 bp
  10% of sequence in fragments shorter than     507 bp
  15% of sequence in fragments shorter than     655 bp
  20% of sequence in fragments shorter than     794 bp
  25% of sequence in fragments shorter than     935 bp
  30% of sequence in fragments shorter than    1072 bp
  35% of sequence in fragments shorter than    1214 bp
  40% of sequence in fragments shorter than    1361 bp
  45% of sequence in fragments shorter than    1513 bp
  50% of sequence in fragments shorter than    1678 bp
  55% of sequence in fragments shorter than    1853 bp
  60% of sequence in fragments shorter than    2043 bp
  65% of sequence in fragments shorter than    2244 bp
  70% of sequence in fragments shorter than    2484 bp
  75% of sequence in fragments shorter than    2736 bp
  80% of sequence in fragments shorter than    3066 bp
  85% of sequence in fragments shorter than    3494 bp
  90% of sequence in fragments shorter than    4105 bp
  95% of sequence in fragments shorter than    5230 bp

# BglII.HpaII (AGATCT+CCGG) double digest, 3092 thousand fragments (774732+2317722 sites), 963 bp average fragment length
   5% of sequence in fragments shorter than     308 bp
  10% of sequence in fragments shorter than     529 bp
  15% of sequence in fragments shorter than     725 bp
  20% of sequence in fragments shorter than     914 bp
  25% of sequence in fragments shorter than    1106 bp
  30% of sequence in fragments shorter than    1298 bp
  35% of sequence in fragments shorter than    1496 bp
  40% of sequence in fragments shorter than    1705 bp
  45% of sequence in fragments shorter than    1931 bp
  50% of sequence in fragments shorter than    2172 bp
  55% of sequence in fragments shorter than    2437 bp
  60% of sequence in fragments shorter than    2732 bp
  65% of sequence in fragments shorter than    3057 bp
  70% of sequence in fragments shorter than    3436 bp
  75% of sequence in fragments shorter than    3883 bp
  80% of sequence in fragments shorter than    4421 bp
  85% of sequence in fragments shorter than    5131 bp
  90% of sequence in fragments shorter than    6160 bp
  95% of sequence in fragments shorter than    8229 bp

# BsaAI.SspI (YACGTR+AATATT) double digest, 3090 thousand fragments (713801+2377267 sites), 964 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     506 bp
  15% of sequence in fragments shorter than     664 bp
  20% of sequence in fragments shorter than     815 bp
  25% of sequence in fragments shorter than     966 bp
  30% of sequence in fragments shorter than    1114 bp
  35% of sequence in fragments shorter than    1271 bp
  40% of sequence in fragments shorter than    1436 bp
  45% of sequence in fragments shorter than    1608 bp
  50% of sequence in fragments shorter than    1799 bp
  55% of sequence in fragments shorter than    2004 bp
  60% of sequence in fragments shorter than    2232 bp
  65% of sequence in fragments shorter than    2486 bp
  70% of sequence in fragments shorter than    2782 bp
  75% of sequence in fragments shorter than    3129 bp
  80% of sequence in fragments shorter than    3547 bp
  85% of sequence in fragments shorter than    4091 bp
  90% of sequence in fragments shorter than    4904 bp
  95% of sequence in fragments shorter than    6485 bp

# AflIII.EsaBC3I (ACRYGT+TCGA) double digest, 3088 thousand fragments (1485394+1603346 sites), 964 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     513 bp
  15% of sequence in fragments shorter than     669 bp
  20% of sequence in fragments shorter than     813 bp
  25% of sequence in fragments shorter than     957 bp
  30% of sequence in fragments shorter than    1100 bp
  35% of sequence in fragments shorter than    1243 bp
  40% of sequence in fragments shorter than    1386 bp
  45% of sequence in fragments shorter than    1543 bp
  50% of sequence in fragments shorter than    1707 bp
  55% of sequence in fragments shorter than    1882 bp
  60% of sequence in fragments shorter than    2070 bp
  65% of sequence in fragments shorter than    2280 bp
  70% of sequence in fragments shorter than    2509 bp
  75% of sequence in fragments shorter than    2779 bp
  80% of sequence in fragments shorter than    3106 bp
  85% of sequence in fragments shorter than    3526 bp
  90% of sequence in fragments shorter than    4097 bp
  95% of sequence in fragments shorter than    5100 bp

# FauI.PsiI (CCCGC+TTATAA) double digest, 3087 thousand fragments (1439772+1647912 sites), 965 bp average fragment length
   5% of sequence in fragments shorter than     336 bp
  10% of sequence in fragments shorter than     536 bp
  15% of sequence in fragments shorter than     709 bp
  20% of sequence in fragments shorter than     874 bp
  25% of sequence in fragments shorter than    1033 bp
  30% of sequence in fragments shorter than    1195 bp
  35% of sequence in fragments shorter than    1361 bp
  40% of sequence in fragments shorter than    1527 bp
  45% of sequence in fragments shorter than    1707 bp
  50% of sequence in fragments shorter than    1896 bp
  55% of sequence in fragments shorter than    2087 bp
  60% of sequence in fragments shorter than    2305 bp
  65% of sequence in fragments shorter than    2547 bp
  70% of sequence in fragments shorter than    2825 bp
  75% of sequence in fragments shorter than    3140 bp
  80% of sequence in fragments shorter than    3535 bp
  85% of sequence in fragments shorter than    4048 bp
  90% of sequence in fragments shorter than    4785 bp
  95% of sequence in fragments shorter than    6148 bp

# BspMI.PsiI (ACCTGC+TTATAA) double digest, 3085 thousand fragments (1438129+1647912 sites), 965 bp average fragment length
   5% of sequence in fragments shorter than     340 bp
  10% of sequence in fragments shorter than     505 bp
  15% of sequence in fragments shorter than     661 bp
  20% of sequence in fragments shorter than     804 bp
  25% of sequence in fragments shorter than     940 bp
  30% of sequence in fragments shorter than    1081 bp
  35% of sequence in fragments shorter than    1225 bp
  40% of sequence in fragments shorter than    1374 bp
  45% of sequence in fragments shorter than    1527 bp
  50% of sequence in fragments shorter than    1692 bp
  55% of sequence in fragments shorter than    1868 bp
  60% of sequence in fragments shorter than    2042 bp
  65% of sequence in fragments shorter than    2252 bp
  70% of sequence in fragments shorter than    2490 bp
  75% of sequence in fragments shorter than    2764 bp
  80% of sequence in fragments shorter than    3071 bp
  85% of sequence in fragments shorter than    3449 bp
  90% of sequence in fragments shorter than    4019 bp
  95% of sequence in fragments shorter than    5014 bp

# AvaII.BstEII (GGWCC+GGTNACC) double digest, 3083 thousand fragments (2747690+335865 sites), 966 bp average fragment length
   5% of sequence in fragments shorter than     313 bp
  10% of sequence in fragments shorter than     495 bp
  15% of sequence in fragments shorter than     672 bp
  20% of sequence in fragments shorter than     837 bp
  25% of sequence in fragments shorter than     989 bp
  30% of sequence in fragments shorter than    1168 bp
  35% of sequence in fragments shorter than    1354 bp
  40% of sequence in fragments shorter than    1547 bp
  45% of sequence in fragments shorter than    1754 bp
  50% of sequence in fragments shorter than    1968 bp
  55% of sequence in fragments shorter than    2207 bp
  60% of sequence in fragments shorter than    2470 bp
  65% of sequence in fragments shorter than    2763 bp
  70% of sequence in fragments shorter than    3102 bp
  75% of sequence in fragments shorter than    3501 bp
  80% of sequence in fragments shorter than    3995 bp
  85% of sequence in fragments shorter than    4634 bp
  90% of sequence in fragments shorter than    5550 bp
  95% of sequence in fragments shorter than    7260 bp

# BceAI.SspI (ACGGC+AATATT) double digest, 3081 thousand fragments (705178+2377267 sites), 966 bp average fragment length
   5% of sequence in fragments shorter than     327 bp
  10% of sequence in fragments shorter than     508 bp
  15% of sequence in fragments shorter than     666 bp
  20% of sequence in fragments shorter than     816 bp
  25% of sequence in fragments shorter than     965 bp
  30% of sequence in fragments shorter than    1112 bp
  35% of sequence in fragments shorter than    1267 bp
  40% of sequence in fragments shorter than    1423 bp
  45% of sequence in fragments shorter than    1590 bp
  50% of sequence in fragments shorter than    1770 bp
  55% of sequence in fragments shorter than    1965 bp
  60% of sequence in fragments shorter than    2181 bp
  65% of sequence in fragments shorter than    2423 bp
  70% of sequence in fragments shorter than    2699 bp
  75% of sequence in fragments shorter than    3022 bp
  80% of sequence in fragments shorter than    3420 bp
  85% of sequence in fragments shorter than    3937 bp
  90% of sequence in fragments shorter than    4643 bp
  95% of sequence in fragments shorter than    5955 bp

# HhaI.PstI (GCGC+CTGCAG) double digest, 3077 thousand fragments (1756502+1321469 sites), 968 bp average fragment length
   5% of sequence in fragments shorter than     314 bp
  10% of sequence in fragments shorter than     524 bp
  15% of sequence in fragments shorter than     709 bp
  20% of sequence in fragments shorter than     909 bp
  25% of sequence in fragments shorter than    1108 bp
  30% of sequence in fragments shorter than    1319 bp
  35% of sequence in fragments shorter than    1541 bp
  40% of sequence in fragments shorter than    1776 bp
  45% of sequence in fragments shorter than    2034 bp
  50% of sequence in fragments shorter than    2313 bp
  55% of sequence in fragments shorter than    2620 bp
  60% of sequence in fragments shorter than    2966 bp
  65% of sequence in fragments shorter than    3359 bp
  70% of sequence in fragments shorter than    3810 bp
  75% of sequence in fragments shorter than    4331 bp
  80% of sequence in fragments shorter than    4961 bp
  85% of sequence in fragments shorter than    5766 bp
  90% of sequence in fragments shorter than    6890 bp
  95% of sequence in fragments shorter than    8849 bp

# HpaII.NcoI (CCGG+CCATGG) double digest, 3076 thousand fragments (2317722+759594 sites), 968 bp average fragment length
   5% of sequence in fragments shorter than     298 bp
  10% of sequence in fragments shorter than     525 bp
  15% of sequence in fragments shorter than     732 bp
  20% of sequence in fragments shorter than     937 bp
  25% of sequence in fragments shorter than    1145 bp
  30% of sequence in fragments shorter than    1362 bp
  35% of sequence in fragments shorter than    1582 bp
  40% of sequence in fragments shorter than    1817 bp
  45% of sequence in fragments shorter than    2070 bp
  50% of sequence in fragments shorter than    2342 bp
  55% of sequence in fragments shorter than    2651 bp
  60% of sequence in fragments shorter than    2990 bp
  65% of sequence in fragments shorter than    3377 bp
  70% of sequence in fragments shorter than    3818 bp
  75% of sequence in fragments shorter than    4324 bp
  80% of sequence in fragments shorter than    4955 bp
  85% of sequence in fragments shorter than    5778 bp
  90% of sequence in fragments shorter than    7008 bp
  95% of sequence in fragments shorter than    9405 bp

# BciVI.SspI (GTATCC+AATATT) double digest, 3072 thousand fragments (696093+2377267 sites), 969 bp average fragment length
   5% of sequence in fragments shorter than     318 bp
  10% of sequence in fragments shorter than     502 bp
  15% of sequence in fragments shorter than     663 bp
  20% of sequence in fragments shorter than     815 bp
  25% of sequence in fragments shorter than     968 bp
  30% of sequence in fragments shorter than    1125 bp
  35% of sequence in fragments shorter than    1288 bp
  40% of sequence in fragments shorter than    1455 bp
  45% of sequence in fragments shorter than    1637 bp
  50% of sequence in fragments shorter than    1824 bp
  55% of sequence in fragments shorter than    2035 bp
  60% of sequence in fragments shorter than    2267 bp
  65% of sequence in fragments shorter than    2537 bp
  70% of sequence in fragments shorter than    2860 bp
  75% of sequence in fragments shorter than    3248 bp
  80% of sequence in fragments shorter than    3749 bp
  85% of sequence in fragments shorter than    4437 bp
  90% of sequence in fragments shorter than    5538 bp
  95% of sequence in fragments shorter than    7933 bp

# BstEII.SmlI (GGTNACC+CTYRAG) double digest, 3063 thousand fragments (335865+2727867 sites), 972 bp average fragment length
   5% of sequence in fragments shorter than     332 bp
  10% of sequence in fragments shorter than     511 bp
  15% of sequence in fragments shorter than     661 bp
  20% of sequence in fragments shorter than     801 bp
  25% of sequence in fragments shorter than     942 bp
  30% of sequence in fragments shorter than    1080 bp
  35% of sequence in fragments shorter than    1223 bp
  40% of sequence in fragments shorter than    1370 bp
  45% of sequence in fragments shorter than    1524 bp
  50% of sequence in fragments shorter than    1691 bp
  55% of sequence in fragments shorter than    1869 bp
  60% of sequence in fragments shorter than    2060 bp
  65% of sequence in fragments shorter than    2269 bp
  70% of sequence in fragments shorter than    2510 bp
  75% of sequence in fragments shorter than    2761 bp
  80% of sequence in fragments shorter than    3097 bp
  85% of sequence in fragments shorter than    3533 bp
  90% of sequence in fragments shorter than    4156 bp
  95% of sequence in fragments shorter than    5360 bp

# BclI.HpaII (TGATCA+CCGG) double digest, 3056 thousand fragments (738785+2317722 sites), 975 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     535 bp
  15% of sequence in fragments shorter than     735 bp
  20% of sequence in fragments shorter than     926 bp
  25% of sequence in fragments shorter than    1121 bp
  30% of sequence in fragments shorter than    1321 bp
  35% of sequence in fragments shorter than    1527 bp
  40% of sequence in fragments shorter than    1737 bp
  45% of sequence in fragments shorter than    1966 bp
  50% of sequence in fragments shorter than    2213 bp
  55% of sequence in fragments shorter than    2483 bp
  60% of sequence in fragments shorter than    2780 bp
  65% of sequence in fragments shorter than    3112 bp
  70% of sequence in fragments shorter than    3499 bp
  75% of sequence in fragments shorter than    3951 bp
  80% of sequence in fragments shorter than    4505 bp
  85% of sequence in fragments shorter than    5241 bp
  90% of sequence in fragments shorter than    6304 bp
  95% of sequence in fragments shorter than    8417 bp

# FnuDII.HpaII (CGCG+CCGG) double digest, 3051 thousand fragments (733940+2317722 sites), 976 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     607 bp
  15% of sequence in fragments shorter than     886 bp
  20% of sequence in fragments shorter than    1168 bp
  25% of sequence in fragments shorter than    1464 bp
  30% of sequence in fragments shorter than    1772 bp
  35% of sequence in fragments shorter than    2107 bp
  40% of sequence in fragments shorter than    2475 bp
  45% of sequence in fragments shorter than    2878 bp
  50% of sequence in fragments shorter than    3317 bp
  55% of sequence in fragments shorter than    3807 bp
  60% of sequence in fragments shorter than    4357 bp
  65% of sequence in fragments shorter than    4992 bp
  70% of sequence in fragments shorter than    5720 bp
  75% of sequence in fragments shorter than    6587 bp
  80% of sequence in fragments shorter than    7668 bp
  85% of sequence in fragments shorter than    9101 bp
  90% of sequence in fragments shorter than   11195 bp
  95% of sequence in fragments shorter than   15237 bp

# AvaII.SexAI (GGWCC+ACCWGGT) double digest, 3045 thousand fragments (2747690+297900 sites), 978 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     507 bp
  15% of sequence in fragments shorter than     686 bp
  20% of sequence in fragments shorter than     859 bp
  25% of sequence in fragments shorter than    1020 bp
  30% of sequence in fragments shorter than    1203 bp
  35% of sequence in fragments shorter than    1389 bp
  40% of sequence in fragments shorter than    1587 bp
  45% of sequence in fragments shorter than    1793 bp
  50% of sequence in fragments shorter than    2015 bp
  55% of sequence in fragments shorter than    2258 bp
  60% of sequence in fragments shorter than    2529 bp
  65% of sequence in fragments shorter than    2820 bp
  70% of sequence in fragments shorter than    3164 bp
  75% of sequence in fragments shorter than    3566 bp
  80% of sequence in fragments shorter than    4067 bp
  85% of sequence in fragments shorter than    4712 bp
  90% of sequence in fragments shorter than    5644 bp
  95% of sequence in fragments shorter than    7362 bp

# EsaBC3I.FauI (TCGA+CCCGC) double digest, 3042 thousand fragments (1603346+1439772 sites), 979 bp average fragment length
   5% of sequence in fragments shorter than     336 bp
  10% of sequence in fragments shorter than     550 bp
  15% of sequence in fragments shorter than     748 bp
  20% of sequence in fragments shorter than     936 bp
  25% of sequence in fragments shorter than    1130 bp
  30% of sequence in fragments shorter than    1327 bp
  35% of sequence in fragments shorter than    1520 bp
  40% of sequence in fragments shorter than    1739 bp
  45% of sequence in fragments shorter than    1971 bp
  50% of sequence in fragments shorter than    2223 bp
  55% of sequence in fragments shorter than    2497 bp
  60% of sequence in fragments shorter than    2794 bp
  65% of sequence in fragments shorter than    3126 bp
  70% of sequence in fragments shorter than    3515 bp
  75% of sequence in fragments shorter than    3970 bp
  80% of sequence in fragments shorter than    4527 bp
  85% of sequence in fragments shorter than    5244 bp
  90% of sequence in fragments shorter than    6259 bp
  95% of sequence in fragments shorter than    7974 bp

# BspMI.EsaBC3I (ACCTGC+TCGA) double digest, 3040 thousand fragments (1438129+1603346 sites), 979 bp average fragment length
   5% of sequence in fragments shorter than     340 bp
  10% of sequence in fragments shorter than     509 bp
  15% of sequence in fragments shorter than     669 bp
  20% of sequence in fragments shorter than     817 bp
  25% of sequence in fragments shorter than     960 bp
  30% of sequence in fragments shorter than    1110 bp
  35% of sequence in fragments shorter than    1262 bp
  40% of sequence in fragments shorter than    1424 bp
  45% of sequence in fragments shorter than    1596 bp
  50% of sequence in fragments shorter than    1779 bp
  55% of sequence in fragments shorter than    1978 bp
  60% of sequence in fragments shorter than    2194 bp
  65% of sequence in fragments shorter than    2430 bp
  70% of sequence in fragments shorter than    2703 bp
  75% of sequence in fragments shorter than    3009 bp
  80% of sequence in fragments shorter than    3378 bp
  85% of sequence in fragments shorter than    3871 bp
  90% of sequence in fragments shorter than    4559 bp
  95% of sequence in fragments shorter than    5747 bp

# AflIII.HgiCI (ACRYGT+GGYRCC) double digest, 3035 thousand fragments (1485394+1550877 sites), 981 bp average fragment length
   5% of sequence in fragments shorter than     341 bp
  10% of sequence in fragments shorter than     517 bp
  15% of sequence in fragments shorter than     678 bp
  20% of sequence in fragments shorter than     828 bp
  25% of sequence in fragments shorter than     968 bp
  30% of sequence in fragments shorter than    1118 bp
  35% of sequence in fragments shorter than    1256 bp
  40% of sequence in fragments shorter than    1406 bp
  45% of sequence in fragments shorter than    1570 bp
  50% of sequence in fragments shorter than    1738 bp
  55% of sequence in fragments shorter than    1925 bp
  60% of sequence in fragments shorter than    2125 bp
  65% of sequence in fragments shorter than    2347 bp
  70% of sequence in fragments shorter than    2602 bp
  75% of sequence in fragments shorter than    2884 bp
  80% of sequence in fragments shorter than    3239 bp
  85% of sequence in fragments shorter than    3706 bp
  90% of sequence in fragments shorter than    4363 bp
  95% of sequence in fragments shorter than    5629 bp

# AvaII.KpnI (GGWCC+GGTACC) double digest, 3035 thousand fragments (2747690+287974 sites), 981 bp average fragment length
   5% of sequence in fragments shorter than     319 bp
  10% of sequence in fragments shorter than     501 bp
  15% of sequence in fragments shorter than     680 bp
  20% of sequence in fragments shorter than     852 bp
  25% of sequence in fragments shorter than    1012 bp
  30% of sequence in fragments shorter than    1190 bp
  35% of sequence in fragments shorter than    1376 bp
  40% of sequence in fragments shorter than    1566 bp
  45% of sequence in fragments shorter than    1773 bp
  50% of sequence in fragments shorter than    1996 bp
  55% of sequence in fragments shorter than    2239 bp
  60% of sequence in fragments shorter than    2507 bp
  65% of sequence in fragments shorter than    2804 bp
  70% of sequence in fragments shorter than    3148 bp
  75% of sequence in fragments shorter than    3551 bp
  80% of sequence in fragments shorter than    4051 bp
  85% of sequence in fragments shorter than    4699 bp
  90% of sequence in fragments shorter than    5630 bp
  95% of sequence in fragments shorter than    7366 bp

# AvaII.NheI (GGWCC+GCTAGC) double digest, 3034 thousand fragments (2747690+287384 sites), 982 bp average fragment length
   5% of sequence in fragments shorter than     318 bp
  10% of sequence in fragments shorter than     495 bp
  15% of sequence in fragments shorter than     674 bp
  20% of sequence in fragments shorter than     848 bp
  25% of sequence in fragments shorter than    1015 bp
  30% of sequence in fragments shorter than    1197 bp
  35% of sequence in fragments shorter than    1378 bp
  40% of sequence in fragments shorter than    1569 bp
  45% of sequence in fragments shorter than    1771 bp
  50% of sequence in fragments shorter than    1989 bp
  55% of sequence in fragments shorter than    2222 bp
  60% of sequence in fragments shorter than    2484 bp
  65% of sequence in fragments shorter than    2777 bp
  70% of sequence in fragments shorter than    3117 bp
  75% of sequence in fragments shorter than    3510 bp
  80% of sequence in fragments shorter than    3996 bp
  85% of sequence in fragments shorter than    4626 bp
  90% of sequence in fragments shorter than    5522 bp
  95% of sequence in fragments shorter than    7150 bp

# AflIII.BsmI (ACRYGT+GAATGC) double digest, 3034 thousand fragments (1485394+1549350 sites), 982 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     531 bp
  15% of sequence in fragments shorter than     719 bp
  20% of sequence in fragments shorter than     892 bp
  25% of sequence in fragments shorter than    1054 bp
  30% of sequence in fragments shorter than    1214 bp
  35% of sequence in fragments shorter than    1377 bp
  40% of sequence in fragments shorter than    1541 bp
  45% of sequence in fragments shorter than    1713 bp
  50% of sequence in fragments shorter than    1896 bp
  55% of sequence in fragments shorter than    2094 bp
  60% of sequence in fragments shorter than    2312 bp
  65% of sequence in fragments shorter than    2548 bp
  70% of sequence in fragments shorter than    2812 bp
  75% of sequence in fragments shorter than    3117 bp
  80% of sequence in fragments shorter than    3487 bp
  85% of sequence in fragments shorter than    3956 bp
  90% of sequence in fragments shorter than    4604 bp
  95% of sequence in fragments shorter than    5714 bp

# BsaAI.HpaII (YACGTR+CCGG) double digest, 3031 thousand fragments (713801+2317722 sites), 983 bp average fragment length
   5% of sequence in fragments shorter than     307 bp
  10% of sequence in fragments shorter than     540 bp
  15% of sequence in fragments shorter than     750 bp
  20% of sequence in fragments shorter than     960 bp
  25% of sequence in fragments shorter than    1166 bp
  30% of sequence in fragments shorter than    1384 bp
  35% of sequence in fragments shorter than    1611 bp
  40% of sequence in fragments shorter than    1850 bp
  45% of sequence in fragments shorter than    2094 bp
  50% of sequence in fragments shorter than    2378 bp
  55% of sequence in fragments shorter than    2688 bp
  60% of sequence in fragments shorter than    3028 bp
  65% of sequence in fragments shorter than    3418 bp
  70% of sequence in fragments shorter than    3848 bp
  75% of sequence in fragments shorter than    4372 bp
  80% of sequence in fragments shorter than    5014 bp
  85% of sequence in fragments shorter than    5842 bp
  90% of sequence in fragments shorter than    7032 bp
  95% of sequence in fragments shorter than    9225 bp

# BfiI.PstI (ACTGGG+CTGCAG) double digest, 3027 thousand fragments (1706594+1321469 sites), 984 bp average fragment length
   5% of sequence in fragments shorter than     328 bp
  10% of sequence in fragments shorter than     510 bp
  15% of sequence in fragments shorter than     674 bp
  20% of sequence in fragments shorter than     828 bp
  25% of sequence in fragments shorter than     984 bp
  30% of sequence in fragments shorter than    1140 bp
  35% of sequence in fragments shorter than    1305 bp
  40% of sequence in fragments shorter than    1468 bp
  45% of sequence in fragments shorter than    1651 bp
  50% of sequence in fragments shorter than    1845 bp
  55% of sequence in fragments shorter than    2049 bp
  60% of sequence in fragments shorter than    2277 bp
  65% of sequence in fragments shorter than    2539 bp
  70% of sequence in fragments shorter than    2802 bp
  75% of sequence in fragments shorter than    3143 bp
  80% of sequence in fragments shorter than    3565 bp
  85% of sequence in fragments shorter than    4106 bp
  90% of sequence in fragments shorter than    4860 bp
  95% of sequence in fragments shorter than    6241 bp

# SexAI.SmlI (ACCWGGT+CTYRAG) double digest, 3025 thousand fragments (297900+2727867 sites), 985 bp average fragment length
   5% of sequence in fragments shorter than     337 bp
  10% of sequence in fragments shorter than     516 bp
  15% of sequence in fragments shorter than     668 bp
  20% of sequence in fragments shorter than     812 bp
  25% of sequence in fragments shorter than     954 bp
  30% of sequence in fragments shorter than    1092 bp
  35% of sequence in fragments shorter than    1237 bp
  40% of sequence in fragments shorter than    1383 bp
  45% of sequence in fragments shorter than    1540 bp
  50% of sequence in fragments shorter than    1709 bp
  55% of sequence in fragments shorter than    1889 bp
  60% of sequence in fragments shorter than    2077 bp
  65% of sequence in fragments shorter than    2293 bp
  70% of sequence in fragments shorter than    2536 bp
  75% of sequence in fragments shorter than    2792 bp
  80% of sequence in fragments shorter than    3129 bp
  85% of sequence in fragments shorter than    3570 bp
  90% of sequence in fragments shorter than    4198 bp
  95% of sequence in fragments shorter than    5396 bp

# BceAI.HpaII (ACGGC+CCGG) double digest, 3022 thousand fragments (705178+2317722 sites), 985 bp average fragment length
   5% of sequence in fragments shorter than     297 bp
  10% of sequence in fragments shorter than     543 bp
  15% of sequence in fragments shorter than     776 bp
  20% of sequence in fragments shorter than    1011 bp
  25% of sequence in fragments shorter than    1252 bp
  30% of sequence in fragments shorter than    1505 bp
  35% of sequence in fragments shorter than    1771 bp
  40% of sequence in fragments shorter than    2063 bp
  45% of sequence in fragments shorter than    2375 bp
  50% of sequence in fragments shorter than    2728 bp
  55% of sequence in fragments shorter than    3105 bp
  60% of sequence in fragments shorter than    3536 bp
  65% of sequence in fragments shorter than    4027 bp
  70% of sequence in fragments shorter than    4596 bp
  75% of sequence in fragments shorter than    5270 bp
  80% of sequence in fragments shorter than    6083 bp
  85% of sequence in fragments shorter than    7177 bp
  90% of sequence in fragments shorter than    8764 bp
  95% of sequence in fragments shorter than   11758 bp

# AflII.SspI (CTTAAG+AATATT) double digest, 3020 thousand fragments (643284+2377267 sites), 986 bp average fragment length
   5% of sequence in fragments shorter than     323 bp
  10% of sequence in fragments shorter than     509 bp
  15% of sequence in fragments shorter than     672 bp
  20% of sequence in fragments shorter than     829 bp
  25% of sequence in fragments shorter than     988 bp
  30% of sequence in fragments shorter than    1149 bp
  35% of sequence in fragments shorter than    1318 bp
  40% of sequence in fragments shorter than    1492 bp
  45% of sequence in fragments shorter than    1682 bp
  50% of sequence in fragments shorter than    1882 bp
  55% of sequence in fragments shorter than    2102 bp
  60% of sequence in fragments shorter than    2337 bp
  65% of sequence in fragments shorter than    2620 bp
  70% of sequence in fragments shorter than    2956 bp
  75% of sequence in fragments shorter than    3373 bp
  80% of sequence in fragments shorter than    3918 bp
  85% of sequence in fragments shorter than    4670 bp
  90% of sequence in fragments shorter than    5904 bp
  95% of sequence in fragments shorter than    8821 bp

# KpnI.SmlI (GGTACC+CTYRAG) double digest, 3015 thousand fragments (287974+2727867 sites), 988 bp average fragment length
   5% of sequence in fragments shorter than     338 bp
  10% of sequence in fragments shorter than     516 bp
  15% of sequence in fragments shorter than     669 bp
  20% of sequence in fragments shorter than     809 bp
  25% of sequence in fragments shorter than     951 bp
  30% of sequence in fragments shorter than    1092 bp
  35% of sequence in fragments shorter than    1238 bp
  40% of sequence in fragments shorter than    1388 bp
  45% of sequence in fragments shorter than    1545 bp
  50% of sequence in fragments shorter than    1706 bp
  55% of sequence in fragments shorter than    1884 bp
  60% of sequence in fragments shorter than    2069 bp
  65% of sequence in fragments shorter than    2281 bp
  70% of sequence in fragments shorter than    2522 bp
  75% of sequence in fragments shorter than    2805 bp
  80% of sequence in fragments shorter than    3148 bp
  85% of sequence in fragments shorter than    3592 bp
  90% of sequence in fragments shorter than    4228 bp
  95% of sequence in fragments shorter than    5429 bp

# NheI.SmlI (GCTAGC+CTYRAG) double digest, 3014 thousand fragments (287384+2727867 sites), 988 bp average fragment length
   5% of sequence in fragments shorter than     340 bp
  10% of sequence in fragments shorter than     521 bp
  15% of sequence in fragments shorter than     674 bp
  20% of sequence in fragments shorter than     818 bp
  25% of sequence in fragments shorter than     963 bp
  30% of sequence in fragments shorter than    1094 bp
  35% of sequence in fragments shorter than    1236 bp
  40% of sequence in fragments shorter than    1384 bp
  45% of sequence in fragments shorter than    1533 bp
  50% of sequence in fragments shorter than    1700 bp
  55% of sequence in fragments shorter than    1874 bp
  60% of sequence in fragments shorter than    2066 bp
  65% of sequence in fragments shorter than    2278 bp
  70% of sequence in fragments shorter than    2519 bp
  75% of sequence in fragments shorter than    2800 bp
  80% of sequence in fragments shorter than    3141 bp
  85% of sequence in fragments shorter than    3582 bp
  90% of sequence in fragments shorter than    4214 bp
  95% of sequence in fragments shorter than    5386 bp

# BciVI.HpaII (GTATCC+CCGG) double digest, 3013 thousand fragments (696093+2317722 sites), 988 bp average fragment length
   5% of sequence in fragments shorter than     316 bp
  10% of sequence in fragments shorter than     547 bp
  15% of sequence in fragments shorter than     751 bp
  20% of sequence in fragments shorter than     951 bp
  25% of sequence in fragments shorter than    1151 bp
  30% of sequence in fragments shorter than    1355 bp
  35% of sequence in fragments shorter than    1566 bp
  40% of sequence in fragments shorter than    1784 bp
  45% of sequence in fragments shorter than    2015 bp
  50% of sequence in fragments shorter than    2261 bp
  55% of sequence in fragments shorter than    2535 bp
  60% of sequence in fragments shorter than    2842 bp
  65% of sequence in fragments shorter than    3183 bp
  70% of sequence in fragments shorter than    3578 bp
  75% of sequence in fragments shorter than    4036 bp
  80% of sequence in fragments shorter than    4602 bp
  85% of sequence in fragments shorter than    5335 bp
  90% of sequence in fragments shorter than    6396 bp
  95% of sequence in fragments shorter than    8372 bp

# HaeII.SspI (RGCGCY+AATATT) double digest, 3006 thousand fragments (630101+2377267 sites), 991 bp average fragment length
   5% of sequence in fragments shorter than     335 bp
  10% of sequence in fragments shorter than     516 bp
  15% of sequence in fragments shorter than     681 bp
  20% of sequence in fragments shorter than     828 bp
  25% of sequence in fragments shorter than     988 bp
  30% of sequence in fragments shorter than    1142 bp
  35% of sequence in fragments shorter than    1306 bp
  40% of sequence in fragments shorter than    1474 bp
  45% of sequence in fragments shorter than    1655 bp
  50% of sequence in fragments shorter than    1850 bp
  55% of sequence in fragments shorter than    2058 bp
  60% of sequence in fragments shorter than    2288 bp
  65% of sequence in fragments shorter than    2543 bp
  70% of sequence in fragments shorter than    2838 bp
  75% of sequence in fragments shorter than    3186 bp
  80% of sequence in fragments shorter than    3610 bp
  85% of sequence in fragments shorter than    4147 bp
  90% of sequence in fragments shorter than    4865 bp
  95% of sequence in fragments shorter than    6145 bp

# FauI.HgiCI (CCCGC+GGYRCC) double digest, 2990 thousand fragments (1439772+1550877 sites), 996 bp average fragment length
   5% of sequence in fragments shorter than     333 bp
  10% of sequence in fragments shorter than     574 bp
  15% of sequence in fragments shorter than     797 bp
  20% of sequence in fragments shorter than    1019 bp
  25% of sequence in fragments shorter than    1225 bp
  30% of sequence in fragments shorter than    1447 bp
  35% of sequence in fragments shorter than    1678 bp
  40% of sequence in fragments shorter than    1933 bp
  45% of sequence in fragments shorter than    2204 bp
  50% of sequence in fragments shorter than    2504 bp
  55% of sequence in fragments shorter than    2825 bp
  60% of sequence in fragments shorter than    3175 bp
  65% of sequence in fragments shorter than    3586 bp
  70% of sequence in fragments shorter than    4063 bp
  75% of sequence in fragments shorter than    4622 bp
  80% of sequence in fragments shorter than    5319 bp
  85% of sequence in fragments shorter than    6238 bp
  90% of sequence in fragments shorter than    7596 bp
  95% of sequence in fragments shorter than   10216 bp

# BsmI.FauI (GAATGC+CCCGC) double digest, 2988 thousand fragments (1549350+1439772 sites), 997 bp average fragment length
   5% of sequence in fragments shorter than     314 bp
  10% of sequence in fragments shorter than     554 bp
  15% of sequence in fragments shorter than     777 bp
  20% of sequence in fragments shorter than     982 bp
  25% of sequence in fragments shorter than    1179 bp
  30% of sequence in fragments shorter than    1380 bp
  35% of sequence in fragments shorter than    1589 bp
  40% of sequence in fragments shorter than    1800 bp
  45% of sequence in fragments shorter than    2025 bp
  50% of sequence in fragments shorter than    2264 bp
  55% of sequence in fragments shorter than    2515 bp
  60% of sequence in fragments shorter than    2788 bp
  65% of sequence in fragments shorter than    3095 bp
  70% of sequence in fragments shorter than    3444 bp
  75% of sequence in fragments shorter than    3849 bp
  80% of sequence in fragments shorter than    4334 bp
  85% of sequence in fragments shorter than    4953 bp
  90% of sequence in fragments shorter than    5820 bp
  95% of sequence in fragments shorter than    7270 bp

# BspMI.HgiCI (ACCTGC+GGYRCC) double digest, 2988 thousand fragments (1438129+1550877 sites), 997 bp average fragment length
   5% of sequence in fragments shorter than     340 bp
  10% of sequence in fragments shorter than     523 bp
  15% of sequence in fragments shorter than     696 bp
  20% of sequence in fragments shorter than     858 bp
  25% of sequence in fragments shorter than    1017 bp
  30% of sequence in fragments shorter than    1182 bp
  35% of sequence in fragments shorter than    1339 bp
  40% of sequence in fragments shorter than    1507 bp
  45% of sequence in fragments shorter than    1702 bp
  50% of sequence in fragments shorter than    1912 bp
  55% of sequence in fragments shorter than    2128 bp
  60% of sequence in fragments shorter than    2378 bp
  65% of sequence in fragments shorter than    2651 bp
  70% of sequence in fragments shorter than    2962 bp
  75% of sequence in fragments shorter than    3335 bp
  80% of sequence in fragments shorter than    3794 bp
  85% of sequence in fragments shorter than    4389 bp
  90% of sequence in fragments shorter than    5232 bp
  95% of sequence in fragments shorter than    6744 bp

# BscGI.HhaI (CCCGT+GCGC) double digest, 2987 thousand fragments (1231070+1756502 sites), 997 bp average fragment length
   5% of sequence in fragments shorter than     297 bp
  10% of sequence in fragments shorter than     539 bp
  15% of sequence in fragments shorter than     793 bp
  20% of sequence in fragments shorter than    1048 bp
  25% of sequence in fragments shorter than    1314 bp
  30% of sequence in fragments shorter than    1592 bp
  35% of sequence in fragments shorter than    1886 bp
  40% of sequence in fragments shorter than    2202 bp
  45% of sequence in fragments shorter than    2546 bp
  50% of sequence in fragments shorter than    2922 bp
  55% of sequence in fragments shorter than    3336 bp
  60% of sequence in fragments shorter than    3796 bp
  65% of sequence in fragments shorter than    4309 bp
  70% of sequence in fragments shorter than    4909 bp
  75% of sequence in fragments shorter than    5602 bp
  80% of sequence in fragments shorter than    6454 bp
  85% of sequence in fragments shorter than    7565 bp
  90% of sequence in fragments shorter than    9155 bp
  95% of sequence in fragments shorter than   11923 bp

# BsmI.BspMI (GAATGC+ACCTGC) double digest, 2986 thousand fragments (1549350+1438129 sites), 997 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     542 bp
  15% of sequence in fragments shorter than     730 bp
  20% of sequence in fragments shorter than     904 bp
  25% of sequence in fragments shorter than    1072 bp
  30% of sequence in fragments shorter than    1230 bp
  35% of sequence in fragments shorter than    1397 bp
  40% of sequence in fragments shorter than    1568 bp
  45% of sequence in fragments shorter than    1751 bp
  50% of sequence in fragments shorter than    1923 bp
  55% of sequence in fragments shorter than    2124 bp
  60% of sequence in fragments shorter than    2349 bp
  65% of sequence in fragments shorter than    2598 bp
  70% of sequence in fragments shorter than    2878 bp
  75% of sequence in fragments shorter than    3201 bp
  80% of sequence in fragments shorter than    3590 bp
  85% of sequence in fragments shorter than    4085 bp
  90% of sequence in fragments shorter than    4769 bp
  95% of sequence in fragments shorter than    5910 bp

# AvaII.BsrBI (GGWCC+CCGCTC) double digest, 2982 thousand fragments (2747690+235523 sites), 999 bp average fragment length
   5% of sequence in fragments shorter than     320 bp
  10% of sequence in fragments shorter than     512 bp
  15% of sequence in fragments shorter than     692 bp
  20% of sequence in fragments shorter than     880 bp
  25% of sequence in fragments shorter than    1052 bp
  30% of sequence in fragments shorter than    1248 bp
  35% of sequence in fragments shorter than    1448 bp
  40% of sequence in fragments shorter than    1659 bp
  45% of sequence in fragments shorter than    1874 bp
  50% of sequence in fragments shorter than    2111 bp
  55% of sequence in fragments shorter than    2369 bp
  60% of sequence in fragments shorter than    2654 bp
  65% of sequence in fragments shorter than    2974 bp
  70% of sequence in fragments shorter than    3341 bp
  75% of sequence in fragments shorter than    3771 bp
  80% of sequence in fragments shorter than    4302 bp
  85% of sequence in fragments shorter than    4996 bp
  90% of sequence in fragments shorter than    5989 bp
  95% of sequence in fragments shorter than    7777 bp

# SacI.SspI (GAGCTC+AATATT) double digest, 2980 thousand fragments (603323+2377267 sites), 999 bp average fragment length
   5% of sequence in fragments shorter than     336 bp
  10% of sequence in fragments shorter than     525 bp
  15% of sequence in fragments shorter than     688 bp
  20% of sequence in fragments shorter than     843 bp
  25% of sequence in fragments shorter than     999 bp
  30% of sequence in fragments shorter than    1153 bp
  35% of sequence in fragments shorter than    1313 bp
  40% of sequence in fragments shorter than    1476 bp
  45% of sequence in fragments shorter than    1651 bp
  50% of sequence in fragments shorter than    1841 bp
  55% of sequence in fragments shorter than    2047 bp
  60% of sequence in fragments shorter than    2273 bp
  65% of sequence in fragments shorter than    2529 bp
  70% of sequence in fragments shorter than    2823 bp
  75% of sequence in fragments shorter than    3176 bp
  80% of sequence in fragments shorter than    3613 bp
  85% of sequence in fragments shorter than    4175 bp
  90% of sequence in fragments shorter than    4954 bp
  95% of sequence in fragments shorter than    6494 bp

# AvaII.KasI (GGWCC+GGCGCC) double digest, 2977 thousand fragments (2747690+230381 sites), 1000 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     511 bp
  15% of sequence in fragments shorter than     690 bp
  20% of sequence in fragments shorter than     872 bp
  25% of sequence in fragments shorter than    1036 bp
  30% of sequence in fragments shorter than    1224 bp
  35% of sequence in fragments shorter than    1416 bp
  40% of sequence in fragments shorter than    1619 bp
  45% of sequence in fragments shorter than    1828 bp
  50% of sequence in fragments shorter than    2057 bp
  55% of sequence in fragments shorter than    2306 bp
  60% of sequence in fragments shorter than    2583 bp
  65% of sequence in fragments shorter than    2883 bp
  70% of sequence in fragments shorter than    3236 bp
  75% of sequence in fragments shorter than    3654 bp
  80% of sequence in fragments shorter than    4168 bp
  85% of sequence in fragments shorter than    4839 bp
  90% of sequence in fragments shorter than    5801 bp
  95% of sequence in fragments shorter than    7594 bp

# AvaII.SwaI (GGWCC+ATTTAAAT) double digest, 2973 thousand fragments (2747690+226602 sites), 1002 bp average fragment length
   5% of sequence in fragments shorter than     331 bp
  10% of sequence in fragments shorter than     517 bp
  15% of sequence in fragments shorter than     691 bp
  20% of sequence in fragments shorter than     866 bp
  25% of sequence in fragments shorter than    1024 bp
  30% of sequence in fragments shorter than    1202 bp
  35% of sequence in fragments shorter than    1382 bp
  40% of sequence in fragments shorter than    1572 bp
  45% of sequence in fragments shorter than    1770 bp
  50% of sequence in fragments shorter than    1980 bp
  55% of sequence in fragments shorter than    2211 bp
  60% of sequence in fragments shorter than    2461 bp
  65% of sequence in fragments shorter than    2734 bp
  70% of sequence in fragments shorter than    3052 bp
  75% of sequence in fragments shorter than    3426 bp
  80% of sequence in fragments shorter than    3884 bp
  85% of sequence in fragments shorter than    4482 bp
  90% of sequence in fragments shorter than    5329 bp
  95% of sequence in fragments shorter than    6921 bp

# AvrII.SspI (CCTAGG+AATATT) double digest, 2971 thousand fragments (594956+2377267 sites), 1002 bp average fragment length
   5% of sequence in fragments shorter than     337 bp
  10% of sequence in fragments shorter than     525 bp
  15% of sequence in fragments shorter than     684 bp
  20% of sequence in fragments shorter than     841 bp
  25% of sequence in fragments shorter than     999 bp
  30% of sequence in fragments shorter than    1156 bp
  35% of sequence in fragments shorter than    1316 bp
  40% of sequence in fragments shorter than    1477 bp
  45% of sequence in fragments shorter than    1653 bp
  50% of sequence in fragments shorter than    1842 bp
  55% of sequence in fragments shorter than    2043 bp
  60% of sequence in fragments shorter than    2272 bp
  65% of sequence in fragments shorter than    2517 bp
  70% of sequence in fragments shorter than    2812 bp
  75% of sequence in fragments shorter than    3167 bp
  80% of sequence in fragments shorter than    3615 bp
  85% of sequence in fragments shorter than    4219 bp
  90% of sequence in fragments shorter than    5127 bp
  95% of sequence in fragments shorter than    7018 bp

# PsiI.PstI (TTATAA+CTGCAG) double digest, 2968 thousand fragments (1647912+1321469 sites), 1003 bp average fragment length
   5% of sequence in fragments shorter than     349 bp
  10% of sequence in fragments shorter than     533 bp
  15% of sequence in fragments shorter than     687 bp
  20% of sequence in fragments shorter than     834 bp
  25% of sequence in fragments shorter than     978 bp
  30% of sequence in fragments shorter than    1122 bp
  35% of sequence in fragments shorter than    1264 bp
  40% of sequence in fragments shorter than    1413 bp
  45% of sequence in fragments shorter than    1575 bp
  50% of sequence in fragments shorter than    1745 bp
  55% of sequence in fragments shorter than    1927 bp
  60% of sequence in fragments shorter than    2122 bp
  65% of sequence in fragments shorter than    2344 bp
  70% of sequence in fragments shorter than    2597 bp
  75% of sequence in fragments shorter than    2891 bp
  80% of sequence in fragments shorter than    3233 bp
  85% of sequence in fragments shorter than    3679 bp
  90% of sequence in fragments shorter than    4303 bp
  95% of sequence in fragments shorter than    5425 bp

# BsrBI.SmlI (CCGCTC+CTYRAG) double digest, 2962 thousand fragments (235523+2727867 sites), 1005 bp average fragment length
   5% of sequence in fragments shorter than     343 bp
  10% of sequence in fragments shorter than     527 bp
  15% of sequence in fragments shorter than     686 bp
  20% of sequence in fragments shorter than     834 bp
  25% of sequence in fragments shorter than     980 bp
  30% of sequence in fragments shorter than    1122 bp
  35% of sequence in fragments shorter than    1268 bp
  40% of sequence in fragments shorter than    1419 bp
  45% of sequence in fragments shorter than    1577 bp
  50% of sequence in fragments shorter than    1749 bp
  55% of sequence in fragments shorter than    1931 bp
  60% of sequence in fragments shorter than    2125 bp
  65% of sequence in fragments shorter than    2348 bp
  70% of sequence in fragments shorter than    2598 bp
  75% of sequence in fragments shorter than    2872 bp
  80% of sequence in fragments shorter than    3225 bp
  85% of sequence in fragments shorter than    3678 bp
  90% of sequence in fragments shorter than    4323 bp
  95% of sequence in fragments shorter than    5534 bp

# AflII.HpaII (CTTAAG+CCGG) double digest, 2960 thousand fragments (643284+2317722 sites), 1006 bp average fragment length
   5% of sequence in fragments shorter than     322 bp
  10% of sequence in fragments shorter than     555 bp
  15% of sequence in fragments shorter than     762 bp
  20% of sequence in fragments shorter than     967 bp
  25% of sequence in fragments shorter than    1171 bp
  30% of sequence in fragments shorter than    1380 bp
  35% of sequence in fragments shorter than    1600 bp
  40% of sequence in fragments shorter than    1822 bp
  45% of sequence in fragments shorter than    2065 bp
  50% of sequence in fragments shorter than    2319 bp
  55% of sequence in fragments shorter than    2605 bp
  60% of sequence in fragments shorter than    2922 bp
  65% of sequence in fragments shorter than    3277 bp
  70% of sequence in fragments shorter than    3680 bp
  75% of sequence in fragments shorter than    4156 bp
  80% of sequence in fragments shorter than    4746 bp
  85% of sequence in fragments shorter than    5531 bp
  90% of sequence in fragments shorter than    6678 bp
  95% of sequence in fragments shorter than    8938 bp

# KasI.SmlI (GGCGCC+CTYRAG) double digest, 2957 thousand fragments (230381+2727867 sites), 1007 bp average fragment length
   5% of sequence in fragments shorter than     341 bp
  10% of sequence in fragments shorter than     523 bp
  15% of sequence in fragments shorter than     681 bp
  20% of sequence in fragments shorter than     829 bp
  25% of sequence in fragments shorter than     975 bp
  30% of sequence in fragments shorter than    1118 bp
  35% of sequence in fragments shorter than    1267 bp
  40% of sequence in fragments shorter than    1419 bp
  45% of sequence in fragments shorter than    1582 bp
  50% of sequence in fragments shorter than    1756 bp
  55% of sequence in fragments shorter than    1942 bp
  60% of sequence in fragments shorter than    2138 bp
  65% of sequence in fragments shorter than    2363 bp
  70% of sequence in fragments shorter than    2617 bp
  75% of sequence in fragments shorter than    2884 bp
  80% of sequence in fragments shorter than    3240 bp
  85% of sequence in fragments shorter than    3699 bp
  90% of sequence in fragments shorter than    4351 bp
  95% of sequence in fragments shorter than    5603 bp

# SmlI.SwaI (CTYRAG+ATTTAAAT) double digest, 2954 thousand fragments (2727867+226602 sites), 1008 bp average fragment length
   5% of sequence in fragments shorter than     345 bp
  10% of sequence in fragments shorter than     529 bp
  15% of sequence in fragments shorter than     686 bp
  20% of sequence in fragments shorter than     833 bp
  25% of sequence in fragments shorter than     977 bp
  30% of sequence in fragments shorter than    1118 bp
  35% of sequence in fragments shorter than    1266 bp
  40% of sequence in fragments shorter than    1415 bp
  45% of sequence in fragments shorter than    1575 bp
  50% of sequence in fragments shorter than    1745 bp
  55% of sequence in fragments shorter than    1926 bp
  60% of sequence in fragments shorter than    2116 bp
  65% of sequence in fragments shorter than    2334 bp
  70% of sequence in fragments shorter than    2581 bp
  75% of sequence in fragments shorter than    2837 bp
  80% of sequence in fragments shorter than    3179 bp
  85% of sequence in fragments shorter than    3624 bp
  90% of sequence in fragments shorter than    4252 bp
  95% of sequence in fragments shorter than    5458 bp

# HgaI.SspI (GACGC+AATATT) double digest, 2948 thousand fragments (571889+2377267 sites), 1010 bp average fragment length
   5% of sequence in fragments shorter than     339 bp
  10% of sequence in fragments shorter than     531 bp
  15% of sequence in fragments shorter than     697 bp
  20% of sequence in fragments shorter than     856 bp
  25% of sequence in fragments shorter than    1017 bp
  30% of sequence in fragments shorter than    1175 bp
  35% of sequence in fragments shorter than    1337 bp
  40% of sequence in fragments shorter than    1504 bp
  45% of sequence in fragments shorter than    1686 bp
  50% of sequence in fragments shorter than    1878 bp
  55% of sequence in fragments shorter than    2086 bp
  60% of sequence in fragments shorter than    2319 bp
  65% of sequence in fragments shorter than    2580 bp
  70% of sequence in fragments shorter than    2878 bp
  75% of sequence in fragments shorter than    3224 bp
  80% of sequence in fragments shorter than    3647 bp
  85% of sequence in fragments shorter than    4195 bp
  90% of sequence in fragments shorter than    4976 bp
  95% of sequence in fragments shorter than    6442 bp

# EciI.SspI (GGCGGA+AATATT) double digest, 2948 thousand fragments (571273+2377267 sites), 1010 bp average fragment length
   5% of sequence in fragments shorter than     331 bp
  10% of sequence in fragments shorter than     520 bp
  15% of sequence in fragments shorter than     686 bp
  20% of sequence in fragments shorter than     844 bp
  25% of sequence in fragments shorter than    1003 bp
  30% of sequence in fragments shorter than    1162 bp
  35% of sequence in fragments shorter than    1328 bp
  40% of sequence in fragments shorter than    1496 bp
  45% of sequence in fragments shorter than    1679 bp
  50% of sequence in fragments shorter than    1877 bp
  55% of sequence in fragments shorter than    2089 bp
  60% of sequence in fragments shorter than    2329 bp
  65% of sequence in fragments shorter than    2597 bp
  70% of sequence in fragments shorter than    2909 bp
  75% of sequence in fragments shorter than    3277 bp
  80% of sequence in fragments shorter than    3736 bp
  85% of sequence in fragments shorter than    4326 bp
  90% of sequence in fragments shorter than    5173 bp
  95% of sequence in fragments shorter than    6870 bp

# HaeII.HpaII (RGCGCY+CCGG) double digest, 2947 thousand fragments (630101+2317722 sites), 1011 bp average fragment length
   5% of sequence in fragments shorter than     321 bp
  10% of sequence in fragments shorter than     575 bp
  15% of sequence in fragments shorter than     820 bp
  20% of sequence in fragments shorter than    1071 bp
  25% of sequence in fragments shorter than    1348 bp
  30% of sequence in fragments shorter than    1636 bp
  35% of sequence in fragments shorter than    1943 bp
  40% of sequence in fragments shorter than    2273 bp
  45% of sequence in fragments shorter than    2645 bp
  50% of sequence in fragments shorter than    3053 bp
  55% of sequence in fragments shorter than    3499 bp
  60% of sequence in fragments shorter than    4006 bp
  65% of sequence in fragments shorter than    4572 bp
  70% of sequence in fragments shorter than    5237 bp
  75% of sequence in fragments shorter than    6009 bp
  80% of sequence in fragments shorter than    6963 bp
  85% of sequence in fragments shorter than    8203 bp
  90% of sequence in fragments shorter than    9967 bp
  95% of sequence in fragments shorter than   13056 bp

# MfeI.SspI (CAATTG+AATATT) double digest, 2941 thousand fragments (564303+2377267 sites), 1013 bp average fragment length
   5% of sequence in fragments shorter than     330 bp
  10% of sequence in fragments shorter than     521 bp
  15% of sequence in fragments shorter than     689 bp
  20% of sequence in fragments shorter than     851 bp
  25% of sequence in fragments shorter than    1016 bp
  30% of sequence in fragments shorter than    1182 bp
  35% of sequence in fragments shorter than    1359 bp
  40% of sequence in fragments shorter than    1537 bp
  45% of sequence in fragments shorter than    1738 bp
  50% of sequence in fragments shorter than    1948 bp
  55% of sequence in fragments shorter than    2181 bp
  60% of sequence in fragments shorter than    2435 bp
  65% of sequence in fragments shorter than    2741 bp
  70% of sequence in fragments shorter than    3105 bp
  75% of sequence in fragments shorter than    3555 bp
  80% of sequence in fragments shorter than    4142 bp
  85% of sequence in fragments shorter than    4966 bp
  90% of sequence in fragments shorter than    6332 bp
  95% of sequence in fragments shorter than    9457 bp

# BfiI.BscGI (ACTGGG+CCCGT) double digest, 2937 thousand fragments (1706594+1231070 sites), 1014 bp average fragment length
   5% of sequence in fragments shorter than     340 bp
  10% of sequence in fragments shorter than     502 bp
  15% of sequence in fragments shorter than     678 bp
  20% of sequence in fragments shorter than     842 bp
  25% of sequence in fragments shorter than    1008 bp
  30% of sequence in fragments shorter than    1180 bp
  35% of sequence in fragments shorter than    1358 bp
  40% of sequence in fragments shorter than    1540 bp
  45% of sequence in fragments shorter than    1735 bp
  50% of sequence in fragments shorter than    1946 bp
  55% of sequence in fragments shorter than    2175 bp
  60% of sequence in fragments shorter than    2423 bp
  65% of sequence in fragments shorter than    2707 bp
  70% of sequence in fragments shorter than    3001 bp
  75% of sequence in fragments shorter than    3376 bp
  80% of sequence in fragments shorter than    3833 bp
  85% of sequence in fragments shorter than    4415 bp
  90% of sequence in fragments shorter than    5219 bp
  95% of sequence in fragments shorter than    6625 bp

# SphI.SspI (GCATGC+AATATT) double digest, 2927 thousand fragments (550952+2377267 sites), 1017 bp average fragment length
   5% of sequence in fragments shorter than     338 bp
  10% of sequence in fragments shorter than     527 bp
  15% of sequence in fragments shorter than     692 bp
  20% of sequence in fragments shorter than     852 bp
  25% of sequence in fragments shorter than    1011 bp
  30% of sequence in fragments shorter than    1172 bp
  35% of sequence in fragments shorter than    1339 bp
  40% of sequence in fragments shorter than    1507 bp
  45% of sequence in fragments shorter than    1694 bp
  50% of sequence in fragments shorter than    1894 bp
  55% of sequence in fragments shorter than    2111 bp
  60% of sequence in fragments shorter than    2356 bp
  65% of sequence in fragments shorter than    2631 bp
  70% of sequence in fragments shorter than    2953 bp
  75% of sequence in fragments shorter than    3338 bp
  80% of sequence in fragments shorter than    3815 bp
  85% of sequence in fragments shorter than    4432 bp
  90% of sequence in fragments shorter than    5349 bp
  95% of sequence in fragments shorter than    7226 bp

# AflIII.FauI (ACRYGT+CCCGC) double digest, 2924 thousand fragments (1485394+1439772 sites), 1018 bp average fragment length
   5% of sequence in fragments shorter than     352 bp
  10% of sequence in fragments shorter than     572 bp
  15% of sequence in fragments shorter than     771 bp
  20% of sequence in fragments shorter than     960 bp
  25% of sequence in fragments shorter than    1140 bp
  30% of sequence in fragments shorter than    1329 bp
  35% of sequence in fragments shorter than    1515 bp
  40% of sequence in fragments shorter than    1715 bp
  45% of sequence in fragments shorter than    1919 bp
  50% of sequence in fragments shorter than    2141 bp
  55% of sequence in fragments shorter than    2379 bp
  60% of sequence in fragments shorter than    2641 bp
  65% of sequence in fragments shorter than    2931 bp
  70% of sequence in fragments shorter than    3257 bp
  75% of sequence in fragments shorter than    3644 bp
  80% of sequence in fragments shorter than    4115 bp
  85% of sequence in fragments shorter than    4694 bp
  90% of sequence in fragments shorter than    5518 bp
  95% of sequence in fragments shorter than    6990 bp

# EsaBC3I.PstI (TCGA+CTGCAG) double digest, 2924 thousand fragments (1603346+1321469 sites), 1019 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     525 bp
  15% of sequence in fragments shorter than     690 bp
  20% of sequence in fragments shorter than     850 bp
  25% of sequence in fragments shorter than    1006 bp
  30% of sequence in fragments shorter than    1165 bp
  35% of sequence in fragments shorter than    1331 bp
  40% of sequence in fragments shorter than    1494 bp
  45% of sequence in fragments shorter than    1679 bp
  50% of sequence in fragments shorter than    1874 bp
  55% of sequence in fragments shorter than    2083 bp
  60% of sequence in fragments shorter than    2318 bp
  65% of sequence in fragments shorter than    2581 bp
  70% of sequence in fragments shorter than    2880 bp
  75% of sequence in fragments shorter than    3226 bp
  80% of sequence in fragments shorter than    3649 bp
  85% of sequence in fragments shorter than    4192 bp
  90% of sequence in fragments shorter than    4957 bp
  95% of sequence in fragments shorter than    6275 bp

# AflIII.BspMI (ACRYGT+ACCTGC) double digest, 2923 thousand fragments (1485394+1438129 sites), 1019 bp average fragment length
   5% of sequence in fragments shorter than     359 bp
  10% of sequence in fragments shorter than     553 bp
  15% of sequence in fragments shorter than     724 bp
  20% of sequence in fragments shorter than     880 bp
  25% of sequence in fragments shorter than    1037 bp
  30% of sequence in fragments shorter than    1195 bp
  35% of sequence in fragments shorter than    1357 bp
  40% of sequence in fragments shorter than    1518 bp
  45% of sequence in fragments shorter than    1693 bp
  50% of sequence in fragments shorter than    1877 bp
  55% of sequence in fragments shorter than    2062 bp
  60% of sequence in fragments shorter than    2270 bp
  65% of sequence in fragments shorter than    2509 bp
  70% of sequence in fragments shorter than    2773 bp
  75% of sequence in fragments shorter than    3051 bp
  80% of sequence in fragments shorter than    3403 bp
  85% of sequence in fragments shorter than    3869 bp
  90% of sequence in fragments shorter than    4517 bp
  95% of sequence in fragments shorter than    5617 bp

# ScaI.SspI (AGTACT+AATATT) double digest, 2920 thousand fragments (543793+2377267 sites), 1020 bp average fragment length
   5% of sequence in fragments shorter than     333 bp
  10% of sequence in fragments shorter than     522 bp
  15% of sequence in fragments shorter than     691 bp
  20% of sequence in fragments shorter than     855 bp
  25% of sequence in fragments shorter than    1022 bp
  30% of sequence in fragments shorter than    1192 bp
  35% of sequence in fragments shorter than    1370 bp
  40% of sequence in fragments shorter than    1549 bp
  45% of sequence in fragments shorter than    1751 bp
  50% of sequence in fragments shorter than    1972 bp
  55% of sequence in fragments shorter than    2217 bp
  60% of sequence in fragments shorter than    2491 bp
  65% of sequence in fragments shorter than    2810 bp
  70% of sequence in fragments shorter than    3195 bp
  75% of sequence in fragments shorter than    3667 bp
  80% of sequence in fragments shorter than    4257 bp
  85% of sequence in fragments shorter than    5060 bp
  90% of sequence in fragments shorter than    6403 bp
  95% of sequence in fragments shorter than    9582 bp

# HpaII.SacI (CCGG+GAGCTC) double digest, 2920 thousand fragments (2317722+603323 sites), 1020 bp average fragment length
   5% of sequence in fragments shorter than     315 bp
  10% of sequence in fragments shorter than     559 bp
  15% of sequence in fragments shorter than     784 bp
  20% of sequence in fragments shorter than    1008 bp
  25% of sequence in fragments shorter than    1235 bp
  30% of sequence in fragments shorter than    1472 bp
  35% of sequence in fragments shorter than    1719 bp
  40% of sequence in fragments shorter than    1986 bp
  45% of sequence in fragments shorter than    2273 bp
  50% of sequence in fragments shorter than    2598 bp
  55% of sequence in fragments shorter than    2956 bp
  60% of sequence in fragments shorter than    3356 bp
  65% of sequence in fragments shorter than    3809 bp
  70% of sequence in fragments shorter than    4316 bp
  75% of sequence in fragments shorter than    4934 bp
  80% of sequence in fragments shorter than    5691 bp
  85% of sequence in fragments shorter than    6678 bp
  90% of sequence in fragments shorter than    8143 bp
  95% of sequence in fragments shorter than   10969 bp

# AclI.AvaII (AACGTT+GGWCC) double digest, 2919 thousand fragments (172706+2747690 sites), 1020 bp average fragment length
   5% of sequence in fragments shorter than     334 bp
  10% of sequence in fragments shorter than     523 bp
  15% of sequence in fragments shorter than     701 bp
  20% of sequence in fragments shorter than     884 bp
  25% of sequence in fragments shorter than    1043 bp
  30% of sequence in fragments shorter than    1231 bp
  35% of sequence in fragments shorter than    1420 bp
  40% of sequence in fragments shorter than    1621 bp
  45% of sequence in fragments shorter than    1828 bp
  50% of sequence in fragments shorter than    2052 bp
  55% of sequence in fragments shorter than    2294 bp
  60% of sequence in fragments shorter than    2566 bp
  65% of sequence in fragments shorter than    2861 bp
  70% of sequence in fragments shorter than    3204 bp
  75% of sequence in fragments shorter than    3606 bp
  80% of sequence in fragments shorter than    4103 bp
  85% of sequence in fragments shorter than    4743 bp
  90% of sequence in fragments shorter than    5642 bp
  95% of sequence in fragments shorter than    7274 bp

# AvrII.HpaII (CCTAGG+CCGG) double digest, 2912 thousand fragments (594956+2317722 sites), 1023 bp average fragment length
   5% of sequence in fragments shorter than     319 bp
  10% of sequence in fragments shorter than     561 bp
  15% of sequence in fragments shorter than     770 bp
  20% of sequence in fragments shorter than     990 bp
  25% of sequence in fragments shorter than    1215 bp
  30% of sequence in fragments shorter than    1447 bp
  35% of sequence in fragments shorter than    1687 bp
  40% of sequence in fragments shorter than    1947 bp
  45% of sequence in fragments shorter than    2227 bp
  50% of sequence in fragments shorter than    2525 bp
  55% of sequence in fragments shorter than    2861 bp
  60% of sequence in fragments shorter than    3231 bp
  65% of sequence in fragments shorter than    3647 bp
  70% of sequence in fragments shorter than    4127 bp
  75% of sequence in fragments shorter than    4688 bp
  80% of sequence in fragments shorter than    5401 bp
  85% of sequence in fragments shorter than    6330 bp
  90% of sequence in fragments shorter than    7724 bp
  95% of sequence in fragments shorter than   10473 bp

# AvaII.PacI (GGWCC+TTAATTAA) double digest, 2906 thousand fragments (2747690+159455 sites), 1025 bp average fragment length
   5% of sequence in fragments shorter than     337 bp
  10% of sequence in fragments shorter than     526 bp
  15% of sequence in fragments shorter than     709 bp
  20% of sequence in fragments shorter than     892 bp
  25% of sequence in fragments shorter than    1056 bp
  30% of sequence in fragments shorter than    1243 bp
  35% of sequence in fragments shorter than    1432 bp
  40% of sequence in fragments shorter than    1632 bp
  45% of sequence in fragments shorter than    1835 bp
  50% of sequence in fragments shorter than    2059 bp
  55% of sequence in fragments shorter than    2301 bp
  60% of sequence in fragments shorter than    2569 bp
  65% of sequence in fragments shorter than    2859 bp
  70% of sequence in fragments shorter than    3198 bp
  75% of sequence in fragments shorter than    3597 bp
  80% of sequence in fragments shorter than    4091 bp
  85% of sequence in fragments shorter than    4728 bp
  90% of sequence in fragments shorter than    5637 bp
  95% of sequence in fragments shorter than    7352 bp

# AvaII.Hpy99I (GGWCC+CGWCG) double digest, 2905 thousand fragments (2747690+157917 sites), 1025 bp average fragment length
   5% of sequence in fragments shorter than     329 bp
  10% of sequence in fragments shorter than     523 bp
  15% of sequence in fragments shorter than     714 bp
  20% of sequence in fragments shorter than     902 bp
  25% of sequence in fragments shorter than    1080 bp
  30% of sequence in fragments shorter than    1278 bp
  35% of sequence in fragments shorter than    1479 bp
  40% of sequence in fragments shorter than    1696 bp
  45% of sequence in fragments shorter than    1915 bp
  50% of sequence in fragments shorter than    2158 bp
  55% of sequence in fragments shorter than    2420 bp
  60% of sequence in fragments shorter than    2704 bp
  65% of sequence in fragments shorter than    3026 bp
  70% of sequence in fragments shorter than    3398 bp
  75% of sequence in fragments shorter than    3837 bp
  80% of sequence in fragments shorter than    4375 bp
  85% of sequence in fragments shorter than    5081 bp
  90% of sequence in fragments shorter than    6085 bp
  95% of sequence in fragments shorter than    7921 bp

# AclI.SmlI (AACGTT+CTYRAG) double digest, 2900 thousand fragments (172706+2727867 sites), 1027 bp average fragment length
   5% of sequence in fragments shorter than     352 bp
  10% of sequence in fragments shorter than     540 bp
  15% of sequence in fragments shorter than     697 bp
  20% of sequence in fragments shorter than     848 bp
  25% of sequence in fragments shorter than     994 bp
  30% of sequence in fragments shorter than    1138 bp
  35% of sequence in fragments shorter than    1288 bp
  40% of sequence in fragments shorter than    1439 bp
  45% of sequence in fragments shorter than    1603 bp
  50% of sequence in fragments shorter than    1775 bp
  55% of sequence in fragments shorter than    1959 bp
  60% of sequence in fragments shorter than    2154 bp
  65% of sequence in fragments shorter than    2376 bp
  70% of sequence in fragments shorter than    2626 bp
  75% of sequence in fragments shorter than    2890 bp
  80% of sequence in fragments shorter than    3240 bp
  85% of sequence in fragments shorter than    3686 bp
  90% of sequence in fragments shorter than    4319 bp
  95% of sequence in fragments shorter than    5497 bp

# HgaI.HpaII (GACGC+CCGG) double digest, 2889 thousand fragments (571889+2317722 sites), 1031 bp average fragment length
   5% of sequence in fragments shorter than     315 bp
  10% of sequence in fragments shorter than     580 bp
  15% of sequence in fragments shorter than     832 bp
  20% of sequence in fragments shorter than    1083 bp
  25% of sequence in fragments shorter than    1346 bp
  30% of sequence in fragments shorter than    1620 bp
  35% of sequence in fragments shorter than    1913 bp
  40% of sequence in fragments shorter than    2227 bp
  45% of sequence in fragments shorter than    2575 bp
  50% of sequence in fragments shorter than    2948 bp
  55% of sequence in fragments shorter than    3366 bp
  60% of sequence in fragments shorter than    3832 bp
  65% of sequence in fragments shorter than    4363 bp
  70% of sequence in fragments shorter than    4989 bp
  75% of sequence in fragments shorter than    5723 bp
  80% of sequence in fragments shorter than    6627 bp
  85% of sequence in fragments shorter than    7835 bp
  90% of sequence in fragments shorter than    9576 bp
  95% of sequence in fragments shorter than   12854 bp

# EciI.HpaII (GGCGGA+CCGG) double digest, 2888 thousand fragments (571273+2317722 sites), 1031 bp average fragment length
   5% of sequence in fragments shorter than     312 bp
  10% of sequence in fragments shorter than     595 bp
  15% of sequence in fragments shorter than     861 bp
  20% of sequence in fragments shorter than    1131 bp
  25% of sequence in fragments shorter than    1411 bp
  30% of sequence in fragments shorter than    1706 bp
  35% of sequence in fragments shorter than    2028 bp
  40% of sequence in fragments shorter than    2374 bp
  45% of sequence in fragments shorter than    2760 bp
  50% of sequence in fragments shorter than    3177 bp
  55% of sequence in fragments shorter than    3648 bp
  60% of sequence in fragments shorter than    4175 bp
  65% of sequence in fragments shorter than    4784 bp
  70% of sequence in fragments shorter than    5488 bp
  75% of sequence in fragments shorter than    6321 bp
  80% of sequence in fragments shorter than    7364 bp
  85% of sequence in fragments shorter than    8757 bp
  90% of sequence in fragments shorter than   10797 bp
  95% of sequence in fragments shorter than   14744 bp

# PacI.SmlI (TTAATTAA+CTYRAG) double digest, 2886 thousand fragments (159455+2727867 sites), 1032 bp average fragment length
   5% of sequence in fragments shorter than     354 bp
  10% of sequence in fragments shorter than     543 bp
  15% of sequence in fragments shorter than     703 bp
  20% of sequence in fragments shorter than     856 bp
  25% of sequence in fragments shorter than    1003 bp
  30% of sequence in fragments shorter than    1150 bp
  35% of sequence in fragments shorter than    1302 bp
  40% of sequence in fragments shorter than    1455 bp
  45% of sequence in fragments shorter than    1621 bp
  50% of sequence in fragments shorter than    1796 bp
  55% of sequence in fragments shorter than    1982 bp
  60% of sequence in fragments shorter than    2176 bp
  65% of sequence in fragments shorter than    2400 bp
  70% of sequence in fragments shorter than    2645 bp
  75% of sequence in fragments shorter than    2919 bp
  80% of sequence in fragments shorter than    3276 bp
  85% of sequence in fragments shorter than    3731 bp
  90% of sequence in fragments shorter than    4383 bp
  95% of sequence in fragments shorter than    5621 bp

# Hpy99I.SmlI (CGWCG+CTYRAG) double digest, 2885 thousand fragments (157917+2727867 sites), 1032 bp average fragment length
   5% of sequence in fragments shorter than     352 bp
  10% of sequence in fragments shorter than     541 bp
  15% of sequence in fragments shorter than     701 bp
  20% of sequence in fragments shorter than     855 bp
  25% of sequence in fragments shorter than    1004 bp
  30% of sequence in fragments shorter than    1152 bp
  35% of sequence in fragments shorter than    1306 bp
  40% of sequence in fragments shorter than    1461 bp
  45% of sequence in fragments shorter than    1629 bp
  50% of sequence in fragments shorter than    1806 bp
  55% of sequence in fragments shorter than    1995 bp
  60% of sequence in fragments shorter than    2194 bp
  65% of sequence in fragments shorter than    2422 bp
  70% of sequence in fragments shorter than    2656 bp
  75% of sequence in fragments shorter than    2949 bp
  80% of sequence in fragments shorter than    3310 bp
  85% of sequence in fragments shorter than    3774 bp
  90% of sequence in fragments shorter than    4434 bp
  95% of sequence in fragments shorter than    5674 bp

# HpaII.MfeI (CCGG+CAATTG) double digest, 2881 thousand fragments (2317722+564303 sites), 1034 bp average fragment length
   5% of sequence in fragments shorter than     334 bp
  10% of sequence in fragments shorter than     577 bp
  15% of sequence in fragments shorter than     790 bp
  20% of sequence in fragments shorter than    1002 bp
  25% of sequence in fragments shorter than    1212 bp
  30% of sequence in fragments shorter than    1428 bp
  35% of sequence in fragments shorter than    1648 bp
  40% of sequence in fragments shorter than    1880 bp
  45% of sequence in fragments shorter than    2120 bp
  50% of sequence in fragments shorter than    2386 bp
  55% of sequence in fragments shorter than    2673 bp
  60% of sequence in fragments shorter than    2993 bp
  65% of sequence in fragments shorter than    3346 bp
  70% of sequence in fragments shorter than    3749 bp
  75% of sequence in fragments shorter than    4221 bp
  80% of sequence in fragments shorter than    4800 bp
  85% of sequence in fragments shorter than    5549 bp
  90% of sequence in fragments shorter than    6620 bp
  95% of sequence in fragments shorter than    8625 bp

# AvaII.SnaBI (GGWCC+TACGTA) double digest, 2879 thousand fragments (2747690+132299 sites), 1034 bp average fragment length
   5% of sequence in fragments shorter than     339 bp
  10% of sequence in fragments shorter than     530 bp
  15% of sequence in fragments shorter than     715 bp
  20% of sequence in fragments shorter than     899 bp
  25% of sequence in fragments shorter than    1067 bp
  30% of sequence in fragments shorter than    1257 bp
  35% of sequence in fragments shorter than    1447 bp
  40% of sequence in fragments shorter than    1650 bp
  45% of sequence in fragments shorter than    1857 bp
  50% of sequence in fragments shorter than    2086 bp
  55% of sequence in fragments shorter than    2336 bp
  60% of sequence in fragments shorter than    2608 bp
  65% of sequence in fragments shorter than    2902 bp
  70% of sequence in fragments shorter than    3248 bp
  75% of sequence in fragments shorter than    3659 bp
  80% of sequence in fragments shorter than    4162 bp
  85% of sequence in fragments shorter than    4812 bp
  90% of sequence in fragments shorter than    5734 bp
  95% of sequence in fragments shorter than    7400 bp

# BscGI.PsiI (CCCGT+TTATAA) double digest, 2878 thousand fragments (1231070+1647912 sites), 1035 bp average fragment length
   5% of sequence in fragments shorter than     345 bp
  10% of sequence in fragments shorter than     523 bp
  15% of sequence in fragments shorter than     693 bp
  20% of sequence in fragments shorter than     857 bp
  25% of sequence in fragments shorter than    1015 bp
  30% of sequence in fragments shorter than    1174 bp
  35% of sequence in fragments shorter than    1334 bp
  40% of sequence in fragments shorter than    1500 bp
  45% of sequence in fragments shorter than    1673 bp
  50% of sequence in fragments shorter than    1857 bp
  55% of sequence in fragments shorter than    2054 bp
  60% of sequence in fragments shorter than    2269 bp
  65% of sequence in fragments shorter than    2506 bp
  70% of sequence in fragments shorter than    2776 bp
  75% of sequence in fragments shorter than    3079 bp
  80% of sequence in fragments shorter than    3455 bp
  85% of sequence in fragments shorter than    3919 bp
  90% of sequence in fragments shorter than    4566 bp
  95% of sequence in fragments shorter than    5681 bp

# BspMI.FauI (ACCTGC+CCCGC) double digest, 2877 thousand fragments (1438129+1439772 sites), 1035 bp average fragment length
   5% of sequence in fragments shorter than     342 bp
  10% of sequence in fragments shorter than     564 bp
  15% of sequence in fragments shorter than     773 bp
  20% of sequence in fragments shorter than     975 bp
  25% of sequence in fragments shorter than    1187 bp
  30% of sequence in fragments shorter than    1390 bp
  35% of sequence in fragments shorter than    1606 bp
  40% of sequence in fragments shorter than    1834 bp
  45% of sequence in fragments shorter than    2071 bp
  50% of sequence in fragments shorter than    2342 bp
  55% of sequence in fragments shorter than    2636 bp
  60% of sequence in fragments shorter than    2960 bp
  65% of sequence in fragments shorter than    3303 bp
  70% of sequence in fragments shorter than    3721 bp
  75% of sequence in fragments shorter than    4213 bp
  80% of sequence in fragments shorter than    4816 bp
  85% of sequence in fragments shorter than    5581 bp
  90% of sequence in fragments shorter than    6681 bp
  95% of sequence in fragments shorter than    8598 bp

# ApaLI.SspI (GTGCAC+AATATT) double digest, 2873 thousand fragments (496255+2377267 sites), 1037 bp average fragment length
   5% of sequence in fragments shorter than     345 bp
  10% of sequence in fragments shorter than     541 bp
  15% of sequence in fragments shorter than     711 bp
  20% of sequence in fragments shorter than     874 bp
  25% of sequence in fragments shorter than    1037 bp
  30% of sequence in fragments shorter than    1202 bp
  35% of sequence in fragments shorter than    1373 bp
  40% of sequence in fragments shorter than    1542 bp
  45% of sequence in fragments shorter than    1724 bp
  50% of sequence in fragments shorter than    1926 bp
  55% of sequence in fragments shorter than    2144 bp
  60% of sequence in fragments shorter than    2390 bp
  65% of sequence in fragments shorter than    2668 bp
  70% of sequence in fragments shorter than    2989 bp
  75% of sequence in fragments shorter than    3375 bp
  80% of sequence in fragments shorter than    3845 bp
  85% of sequence in fragments shorter than    4412 bp
  90% of sequence in fragments shorter than    5338 bp
  95% of sequence in fragments shorter than    7208 bp

# AvaII.NaeI (GGWCC+GCCGGC) double digest, 2872 thousand fragments (2747690+125692 sites), 1037 bp average fragment length
   5% of sequence in fragments shorter than     333 bp
  10% of sequence in fragments shorter than     529 bp
  15% of sequence in fragments shorter than     722 bp
  20% of sequence in fragments shorter than     910 bp
  25% of sequence in fragments shorter than    1093 bp
  30% of sequence in fragments shorter than    1294 bp
  35% of sequence in fragments shorter than    1499 bp
  40% of sequence in fragments shorter than    1719 bp
  45% of sequence in fragments shorter than    1939 bp
  50% of sequence in fragments shorter than    2186 bp
  55% of sequence in fragments shorter than    2452 bp
  60% of sequence in fragments shorter than    2740 bp
  65% of sequence in fragments shorter than    3070 bp
  70% of sequence in fragments shorter than    3449 bp
  75% of sequence in fragments shorter than    3895 bp
  80% of sequence in fragments shorter than    4447 bp
  85% of sequence in fragments shorter than    5166 bp
  90% of sequence in fragments shorter than    6200 bp
  95% of sequence in fragments shorter than    8105 bp

# HgiCI.PstI (GGYRCC+CTGCAG) double digest, 2871 thousand fragments (1550877+1321469 sites), 1037 bp average fragment length
   5% of sequence in fragments shorter than     340 bp
  10% of sequence in fragments shorter than     533 bp
  15% of sequence in fragments shorter than     705 bp
  20% of sequence in fragments shorter than     878 bp
  25% of sequence in fragments shorter than    1051 bp
  30% of sequence in fragments shorter than    1221 bp
  35% of sequence in fragments shorter than    1398 bp
  40% of sequence in fragments shorter than    1585 bp
  45% of sequence in fragments shorter than    1792 bp
  50% of sequence in fragments shorter than    2016 bp
  55% of sequence in fragments shorter than    2248 bp
  60% of sequence in fragments shorter than    2518 bp
  65% of sequence in fragments shorter than    2810 bp
  70% of sequence in fragments shorter than    3153 bp
  75% of sequence in fragments shorter than    3563 bp
  80% of sequence in fragments shorter than    4071 bp
  85% of sequence in fragments shorter than    4736 bp
  90% of sequence in fragments shorter than    5698 bp
  95% of sequence in fragments shorter than    7512 bp

# BsmI.PstI (GAATGC+CTGCAG) double digest, 2870 thousand fragments (1549350+1321469 sites), 1038 bp average fragment length
   5% of sequence in fragments shorter than     328 bp
  10% of sequence in fragments shorter than     553 bp
  15% of sequence in fragments shorter than     752 bp
  20% of sequence in fragments shorter than     934 bp
  25% of sequence in fragments shorter than    1107 bp
  30% of sequence in fragments shorter than    1285 bp
  35% of sequence in fragments shorter than    1462 bp
  40% of sequence in fragments shorter than    1650 bp
  45% of sequence in fragments shorter than    1845 bp
  50% of sequence in fragments shorter than    2051 bp
  55% of sequence in fragments shorter than    2269 bp
  60% of sequence in fragments shorter than    2500 bp
  65% of sequence in fragments shorter than    2756 bp
  70% of sequence in fragments shorter than    3051 bp
  75% of sequence in fragments shorter than    3386 bp
  80% of sequence in fragments shorter than    3795 bp
  85% of sequence in fragments shorter than    4314 bp
  90% of sequence in fragments shorter than    5033 bp
  95% of sequence in fragments shorter than    6255 bp

# AvaII.XhoI (GGWCC+CTCGAG) double digest, 2868 thousand fragments (2747690+121308 sites), 1038 bp average fragment length
   5% of sequence in fragments shorter than     337 bp
  10% of sequence in fragments shorter than     527 bp
  15% of sequence in fragments shorter than     716 bp
  20% of sequence in fragments shorter than     901 bp
  25% of sequence in fragments shorter than    1073 bp
  30% of sequence in fragments shorter than    1269 bp
  35% of sequence in fragments shorter than    1469 bp
  40% of sequence in fragments shorter than    1683 bp
  45% of sequence in fragments shorter than    1899 bp
  50% of sequence in fragments shorter than    2140 bp
  55% of sequence in fragments shorter than    2399 bp
  60% of sequence in fragments shorter than    2686 bp
  65% of sequence in fragments shorter than    3009 bp
  70% of sequence in fragments shorter than    3379 bp
  75% of sequence in fragments shorter than    3815 bp
  80% of sequence in fragments shorter than    4353 bp
  85% of sequence in fragments shorter than    5058 bp
  90% of sequence in fragments shorter than    6063 bp
  95% of sequence in fragments shorter than    7917 bp

# HpaII.SphI (CCGG+GCATGC) double digest, 2868 thousand fragments (2317722+550952 sites), 1038 bp average fragment length
   5% of sequence in fragments shorter than     325 bp
  10% of sequence in fragments shorter than     573 bp
  15% of sequence in fragments shorter than     802 bp
  20% of sequence in fragments shorter than    1030 bp
  25% of sequence in fragments shorter than    1263 bp
  30% of sequence in fragments shorter than    1504 bp
  35% of sequence in fragments shorter than    1756 bp
  40% of sequence in fragments shorter than    2028 bp
  45% of sequence in fragments shorter than    2321 bp
  50% of sequence in fragments shorter than    2649 bp
  55% of sequence in fragments shorter than    3005 bp
  60% of sequence in fragments shorter than    3406 bp
  65% of sequence in fragments shorter than    3861 bp
  70% of sequence in fragments shorter than    4376 bp
  75% of sequence in fragments shorter than    5003 bp
  80% of sequence in fragments shorter than    5766 bp
  85% of sequence in fragments shorter than    6763 bp
  90% of sequence in fragments shorter than    8232 bp
  95% of sequence in fragments shorter than   11017 bp

# HpaII.ScaI (CCGG+AGTACT) double digest, 2861 thousand fragments (2317722+543793 sites), 1041 bp average fragment length
   5% of sequence in fragments shorter than     333 bp
  10% of sequence in fragments shorter than     578 bp
  15% of sequence in fragments shorter than     798 bp
  20% of sequence in fragments shorter than    1013 bp
  25% of sequence in fragments shorter than    1229 bp
  30% of sequence in fragments shorter than    1449 bp
  35% of sequence in fragments shorter than    1677 bp
  40% of sequence in fragments shorter than    1916 bp
  45% of sequence in fragments shorter than    2174 bp
  50% of sequence in fragments shorter than    2455 bp
  55% of sequence in fragments shorter than    2759 bp
  60% of sequence in fragments shorter than    3099 bp
  65% of sequence in fragments shorter than    3480 bp
  70% of sequence in fragments shorter than    3921 bp
  75% of sequence in fragments shorter than    4440 bp
  80% of sequence in fragments shorter than    5083 bp
  85% of sequence in fragments shorter than    5911 bp
  90% of sequence in fragments shorter than    7136 bp
  95% of sequence in fragments shorter than    9499 bp

# SmlI.SnaBI (CTYRAG+TACGTA) double digest, 2859 thousand fragments (2727867+132299 sites), 1042 bp average fragment length
   5% of sequence in fragments shorter than     357 bp
  10% of sequence in fragments shorter than     547 bp
  15% of sequence in fragments shorter than     708 bp
  20% of sequence in fragments shorter than     862 bp
  25% of sequence in fragments shorter than    1010 bp
  30% of sequence in fragments shorter than    1158 bp
  35% of sequence in fragments shorter than    1311 bp
  40% of sequence in fragments shorter than    1462 bp
  45% of sequence in fragments shorter than    1630 bp
  50% of sequence in fragments shorter than    1805 bp
  55% of sequence in fragments shorter than    1991 bp
  60% of sequence in fragments shorter than    2189 bp
  65% of sequence in fragments shorter than    2415 bp
  70% of sequence in fragments shorter than    2650 bp
  75% of sequence in fragments shorter than    2934 bp
  80% of sequence in fragments shorter than    3289 bp
  85% of sequence in fragments shorter than    3741 bp
  90% of sequence in fragments shorter than    4382 bp
  95% of sequence in fragments shorter than    5574 bp

# AccI.HhaI (GTMKAC+GCGC) double digest, 2857 thousand fragments (1101063+1756502 sites), 1043 bp average fragment length
   5% of sequence in fragments shorter than     348 bp
  10% of sequence in fragments shorter than     587 bp
  15% of sequence in fragments shorter than     787 bp
  20% of sequence in fragments shorter than     984 bp
  25% of sequence in fragments shorter than    1175 bp
  30% of sequence in fragments shorter than    1371 bp
  35% of sequence in fragments shorter than    1570 bp
  40% of sequence in fragments shorter than    1775 bp
  45% of sequence in fragments shorter than    1993 bp
  50% of sequence in fragments shorter than    2224 bp
  55% of sequence in fragments shorter than    2474 bp
  60% of sequence in fragments shorter than    2746 bp
  65% of sequence in fragments shorter than    3051 bp
  70% of sequence in fragments shorter than    3397 bp
  75% of sequence in fragments shorter than    3796 bp
  80% of sequence in fragments shorter than    4269 bp
  85% of sequence in fragments shorter than    4880 bp
  90% of sequence in fragments shorter than    5683 bp
  95% of sequence in fragments shorter than    7063 bp

# NaeI.SmlI (GCCGGC+CTYRAG) double digest, 2853 thousand fragments (125692+2727867 sites), 1044 bp average fragment length
   5% of sequence in fragments shorter than     356 bp
  10% of sequence in fragments shorter than     547 bp
  15% of sequence in fragments shorter than     709 bp
  20% of sequence in fragments shorter than     865 bp
  25% of sequence in fragments shorter than    1014 bp
  30% of sequence in fragments shorter than    1164 bp
  35% of sequence in fragments shorter than    1320 bp
  40% of sequence in fragments shorter than    1476 bp
  45% of sequence in fragments shorter than    1646 bp
  50% of sequence in fragments shorter than    1824 bp
  55% of sequence in fragments shorter than    2014 bp
  60% of sequence in fragments shorter than    2216 bp
  65% of sequence in fragments shorter than    2446 bp
  70% of sequence in fragments shorter than    2684 bp
  75% of sequence in fragments shorter than    2982 bp
  80% of sequence in fragments shorter than    3349 bp
  85% of sequence in fragments shorter than    3818 bp
  90% of sequence in fragments shorter than    4497 bp
  95% of sequence in fragments shorter than    5774 bp

# HhaI.PpuMI (GCGC+RGGWCCY) double digest, 2841 thousand fragments (1756502+1085138 sites), 1048 bp average fragment length
   5% of sequence in fragments shorter than     342 bp
  10% of sequence in fragments shorter than     576 bp
  15% of sequence in fragments shorter than     791 bp
  20% of sequence in fragments shorter than    1017 bp
  25% of sequence in fragments shorter than    1243 bp
  30% of sequence in fragments shorter than    1483 bp
  35% of sequence in fragments shorter than    1729 bp
  40% of sequence in fragments shorter than    1988 bp
  45% of sequence in fragments shorter than    2272 bp
  50% of sequence in fragments shorter than    2576 bp
  55% of sequence in fragments shorter than    2915 bp
  60% of sequence in fragments shorter than    3285 bp
  65% of sequence in fragments shorter than    3678 bp
  70% of sequence in fragments shorter than    4152 bp
  75% of sequence in fragments shorter than    4724 bp
  80% of sequence in fragments shorter than    5418 bp
  85% of sequence in fragments shorter than    6316 bp
  90% of sequence in fragments shorter than    7587 bp
  95% of sequence in fragments shorter than    9769 bp

# HhaI.PvuII (GCGC+CAGCTG) double digest, 2840 thousand fragments (1756502+1084260 sites), 1049 bp average fragment length
   5% of sequence in fragments shorter than     343 bp
  10% of sequence in fragments shorter than     580 bp
  15% of sequence in fragments shorter than     788 bp
  20% of sequence in fragments shorter than    1004 bp
  25% of sequence in fragments shorter than    1221 bp
  30% of sequence in fragments shorter than    1451 bp
  35% of sequence in fragments shorter than    1697 bp
  40% of sequence in fragments shorter than    1950 bp
  45% of sequence in fragments shorter than    2226 bp
  50% of sequence in fragments shorter than    2526 bp
  55% of sequence in fragments shorter than    2853 bp
  60% of sequence in fragments shorter than    3221 bp
  65% of sequence in fragments shorter than    3635 bp
  70% of sequence in fragments shorter than    4118 bp
  75% of sequence in fragments shorter than    4681 bp
  80% of sequence in fragments shorter than    5367 bp
  85% of sequence in fragments shorter than    6242 bp
  90% of sequence in fragments shorter than    7470 bp
  95% of sequence in fragments shorter than    9653 bp

# ApaI.SspI (GGGCCC+AATATT) double digest, 2837 thousand fragments (460339+2377267 sites), 1050 bp average fragment length
   5% of sequence in fragments shorter than     354 bp
  10% of sequence in fragments shorter than     553 bp
  15% of sequence in fragments shorter than     725 bp
  20% of sequence in fragments shorter than     890 bp
  25% of sequence in fragments shorter than    1052 bp
  30% of sequence in fragments shorter than    1218 bp
  35% of sequence in fragments shorter than    1384 bp
  40% of sequence in fragments shorter than    1554 bp
  45% of sequence in fragments shorter than    1738 bp
  50% of sequence in fragments shorter than    1936 bp
  55% of sequence in fragments shorter than    2148 bp
  60% of sequence in fragments shorter than    2384 bp
  65% of sequence in fragments shorter than    2648 bp
  70% of sequence in fragments shorter than    2951 bp
  75% of sequence in fragments shorter than    3315 bp
  80% of sequence in fragments shorter than    3763 bp
  85% of sequence in fragments shorter than    4329 bp
  90% of sequence in fragments shorter than    5134 bp
  95% of sequence in fragments shorter than    6711 bp

# BscGI.EsaBC3I (CCCGT+TCGA) double digest, 2833 thousand fragments (1231070+1603346 sites), 1051 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     536 bp
  15% of sequence in fragments shorter than     725 bp
  20% of sequence in fragments shorter than     910 bp
  25% of sequence in fragments shorter than    1096 bp
  30% of sequence in fragments shorter than    1286 bp
  35% of sequence in fragments shorter than    1480 bp
  40% of sequence in fragments shorter than    1687 bp
  45% of sequence in fragments shorter than    1903 bp
  50% of sequence in fragments shorter than    2138 bp
  55% of sequence in fragments shorter than    2393 bp
  60% of sequence in fragments shorter than    2673 bp
  65% of sequence in fragments shorter than    2988 bp
  70% of sequence in fragments shorter than    3348 bp
  75% of sequence in fragments shorter than    3770 bp
  80% of sequence in fragments shorter than    4274 bp
  85% of sequence in fragments shorter than    4925 bp
  90% of sequence in fragments shorter than    5829 bp
  95% of sequence in fragments shorter than    7358 bp

# AvaII.ClaI (GGWCC+ATCGAT) double digest, 2833 thousand fragments (2747690+86477 sites), 1051 bp average fragment length
   5% of sequence in fragments shorter than     341 bp
  10% of sequence in fragments shorter than     536 bp
  15% of sequence in fragments shorter than     725 bp
  20% of sequence in fragments shorter than     908 bp
  25% of sequence in fragments shorter than    1089 bp
  30% of sequence in fragments shorter than    1282 bp
  35% of sequence in fragments shorter than    1481 bp
  40% of sequence in fragments shorter than    1695 bp
  45% of sequence in fragments shorter than    1910 bp
  50% of sequence in fragments shorter than    2149 bp
  55% of sequence in fragments shorter than    2407 bp
  60% of sequence in fragments shorter than    2687 bp
  65% of sequence in fragments shorter than    3006 bp
  70% of sequence in fragments shorter than    3369 bp
  75% of sequence in fragments shorter than    3795 bp
  80% of sequence in fragments shorter than    4325 bp
  85% of sequence in fragments shorter than    5014 bp
  90% of sequence in fragments shorter than    6001 bp
  95% of sequence in fragments shorter than    7820 bp

# EcoRV.SspI (GATATC+AATATT) double digest, 2823 thousand fragments (446473+2377267 sites), 1055 bp average fragment length
   5% of sequence in fragments shorter than     343 bp
  10% of sequence in fragments shorter than     540 bp
  15% of sequence in fragments shorter than     716 bp
  20% of sequence in fragments shorter than     886 bp
  25% of sequence in fragments shorter than    1057 bp
  30% of sequence in fragments shorter than    1232 bp
  35% of sequence in fragments shorter than    1411 bp
  40% of sequence in fragments shorter than    1606 bp
  45% of sequence in fragments shorter than    1821 bp
  50% of sequence in fragments shorter than    2052 bp
  55% of sequence in fragments shorter than    2309 bp
  60% of sequence in fragments shorter than    2601 bp
  65% of sequence in fragments shorter than    2936 bp
  70% of sequence in fragments shorter than    3326 bp
  75% of sequence in fragments shorter than    3809 bp
  80% of sequence in fragments shorter than    4440 bp
  85% of sequence in fragments shorter than    5344 bp
  90% of sequence in fragments shorter than    6833 bp
  95% of sequence in fragments shorter than   10466 bp

# AatII.AvaII (GACGTC+GGWCC) double digest, 2818 thousand fragments (71089+2747690 sites), 1057 bp average fragment length
   5% of sequence in fragments shorter than     341 bp
  10% of sequence in fragments shorter than     538 bp
  15% of sequence in fragments shorter than     731 bp
  20% of sequence in fragments shorter than     918 bp
  25% of sequence in fragments shorter than    1100 bp
  30% of sequence in fragments shorter than    1299 bp
  35% of sequence in fragments shorter than    1503 bp
  40% of sequence in fragments shorter than    1721 bp
  45% of sequence in fragments shorter than    1940 bp
  50% of sequence in fragments shorter than    2186 bp
  55% of sequence in fragments shorter than    2450 bp
  60% of sequence in fragments shorter than    2736 bp
  65% of sequence in fragments shorter than    3064 bp
  70% of sequence in fragments shorter than    3440 bp
  75% of sequence in fragments shorter than    3881 bp
  80% of sequence in fragments shorter than    4428 bp
  85% of sequence in fragments shorter than    5138 bp
  90% of sequence in fragments shorter than    6160 bp
  95% of sequence in fragments shorter than    8031 bp

# AvaII.SacII (GGWCC+CCGCGG) double digest, 2814 thousand fragments (2747690+66901 sites), 1058 bp average fragment length
   5% of sequence in fragments shorter than     341 bp
  10% of sequence in fragments shorter than     542 bp
  15% of sequence in fragments shorter than     740 bp
  20% of sequence in fragments shorter than     929 bp
  25% of sequence in fragments shorter than    1118 bp
  30% of sequence in fragments shorter than    1322 bp
  35% of sequence in fragments shorter than    1530 bp
  40% of sequence in fragments shorter than    1748 bp
  45% of sequence in fragments shorter than    1977 bp
  50% of sequence in fragments shorter than    2227 bp
  55% of sequence in fragments shorter than    2498 bp
  60% of sequence in fragments shorter than    2788 bp
  65% of sequence in fragments shorter than    3124 bp
  70% of sequence in fragments shorter than    3507 bp
  75% of sequence in fragments shorter than    3962 bp
  80% of sequence in fragments shorter than    4522 bp
  85% of sequence in fragments shorter than    5246 bp
  90% of sequence in fragments shorter than    6301 bp
  95% of sequence in fragments shorter than    8223 bp

# ClaI.SmlI (ATCGAT+CTYRAG) double digest, 2813 thousand fragments (86477+2727867 sites), 1059 bp average fragment length
   5% of sequence in fragments shorter than     362 bp
  10% of sequence in fragments shorter than     554 bp
  15% of sequence in fragments shorter than     717 bp
  20% of sequence in fragments shorter than     874 bp
  25% of sequence in fragments shorter than    1024 bp
  30% of sequence in fragments shorter than    1176 bp
  35% of sequence in fragments shorter than    1332 bp
  40% of sequence in fragments shorter than    1488 bp
  45% of sequence in fragments shorter than    1659 bp
  50% of sequence in fragments shorter than    1836 bp
  55% of sequence in fragments shorter than    2026 bp
  60% of sequence in fragments shorter than    2225 bp
  65% of sequence in fragments shorter than    2453 bp
  70% of sequence in fragments shorter than    2696 bp
  75% of sequence in fragments shorter than    2992 bp
  80% of sequence in fragments shorter than    3357 bp
  85% of sequence in fragments shorter than    3824 bp
  90% of sequence in fragments shorter than    4498 bp
  95% of sequence in fragments shorter than    5761 bp

# ApaLI.HpaII (GTGCAC+CCGG) double digest, 2813 thousand fragments (496255+2317722 sites), 1059 bp average fragment length
   5% of sequence in fragments shorter than     328 bp
  10% of sequence in fragments shorter than     585 bp
  15% of sequence in fragments shorter than     823 bp
  20% of sequence in fragments shorter than    1060 bp
  25% of sequence in fragments shorter than    1302 bp
  30% of sequence in fragments shorter than    1555 bp
  35% of sequence in fragments shorter than    1818 bp
  40% of sequence in fragments shorter than    2097 bp
  45% of sequence in fragments shorter than    2406 bp
  50% of sequence in fragments shorter than    2735 bp
  55% of sequence in fragments shorter than    3104 bp
  60% of sequence in fragments shorter than    3513 bp
  65% of sequence in fragments shorter than    3986 bp
  70% of sequence in fragments shorter than    4511 bp
  75% of sequence in fragments shorter than    5147 bp
  80% of sequence in fragments shorter than    5909 bp
  85% of sequence in fragments shorter than    6932 bp
  90% of sequence in fragments shorter than    8445 bp
  95% of sequence in fragments shorter than   11331 bp

# AccI.BfiI (GTMKAC+ACTGGG) double digest, 2807 thousand fragments (1101063+1706594 sites), 1061 bp average fragment length
   5% of sequence in fragments shorter than     369 bp
  10% of sequence in fragments shorter than     557 bp
  15% of sequence in fragments shorter than     727 bp
  20% of sequence in fragments shorter than     887 bp
  25% of sequence in fragments shorter than    1040 bp
  30% of sequence in fragments shorter than    1197 bp
  35% of sequence in fragments shorter than    1354 bp
  40% of sequence in fragments shorter than    1513 bp
  45% of sequence in fragments shorter than    1683 bp
  50% of sequence in fragments shorter than    1861 bp
  55% of sequence in fragments shorter than    2048 bp
  60% of sequence in fragments shorter than    2241 bp
  65% of sequence in fragments shorter than    2472 bp
  70% of sequence in fragments shorter than    2730 bp
  75% of sequence in fragments shorter than    3003 bp
  80% of sequence in fragments shorter than    3358 bp
  85% of sequence in fragments shorter than    3819 bp
  90% of sequence in fragments shorter than    4466 bp
  95% of sequence in fragments shorter than    5580 bp

# AvaII.PspXI (GGWCC+VCTCGAGB) double digest, 2806 thousand fragments (2747690+59193 sites), 1061 bp average fragment length
   5% of sequence in fragments shorter than     342 bp
  10% of sequence in fragments shorter than     541 bp
  15% of sequence in fragments shorter than     734 bp
  20% of sequence in fragments shorter than     922 bp
  25% of sequence in fragments shorter than    1106 bp
  30% of sequence in fragments shorter than    1306 bp
  35% of sequence in fragments shorter than    1512 bp
  40% of sequence in fragments shorter than    1730 bp
  45% of sequence in fragments shorter than    1952 bp
  50% of sequence in fragments shorter than    2199 bp
  55% of sequence in fragments shorter than    2465 bp
  60% of sequence in fragments shorter than    2751 bp
  65% of sequence in fragments shorter than    3082 bp
  70% of sequence in fragments shorter than    3461 bp
  75% of sequence in fragments shorter than    3906 bp
  80% of sequence in fragments shorter than    4458 bp
  85% of sequence in fragments shorter than    5175 bp
  90% of sequence in fragments shorter than    6205 bp
  95% of sequence in fragments shorter than    8098 bp

# AflIII.PstI (ACRYGT+CTGCAG) double digest, 2806 thousand fragments (1485394+1321469 sites), 1061 bp average fragment length
   5% of sequence in fragments shorter than     373 bp
  10% of sequence in fragments shorter than     569 bp
  15% of sequence in fragments shorter than     738 bp
  20% of sequence in fragments shorter than     901 bp
  25% of sequence in fragments shorter than    1061 bp
  30% of sequence in fragments shorter than    1219 bp
  35% of sequence in fragments shorter than    1381 bp
  40% of sequence in fragments shorter than    1552 bp
  45% of sequence in fragments shorter than    1732 bp
  50% of sequence in fragments shorter than    1918 bp
  55% of sequence in fragments shorter than    2123 bp
  60% of sequence in fragments shorter than    2344 bp
  65% of sequence in fragments shorter than    2588 bp
  70% of sequence in fragments shorter than    2867 bp
  75% of sequence in fragments shorter than    3189 bp
  80% of sequence in fragments shorter than    3573 bp
  85% of sequence in fragments shorter than    4060 bp
  90% of sequence in fragments shorter than    4684 bp
  95% of sequence in fragments shorter than    5854 bp

# AgeI.AvaII (ACCGGT+GGWCC) double digest, 2800 thousand fragments (53386+2747690 sites), 1064 bp average fragment length
   5% of sequence in fragments shorter than     343 bp
  10% of sequence in fragments shorter than     543 bp
  15% of sequence in fragments shorter than     736 bp
  20% of sequence in fragments shorter than     924 bp
  25% of sequence in fragments shorter than    1106 bp
  30% of sequence in fragments shorter than    1305 bp
  35% of sequence in fragments shorter than    1510 bp
  40% of sequence in fragments shorter than    1726 bp
  45% of sequence in fragments shorter than    1951 bp
  50% of sequence in fragments shorter than    2198 bp
  55% of sequence in fragments shorter than    2464 bp
  60% of sequence in fragments shorter than    2755 bp
  65% of sequence in fragments shorter than    3085 bp
  70% of sequence in fragments shorter than    3464 bp
  75% of sequence in fragments shorter than    3912 bp
  80% of sequence in fragments shorter than    4464 bp
  85% of sequence in fragments shorter than    5179 bp
  90% of sequence in fragments shorter than    6212 bp
  95% of sequence in fragments shorter than    8110 bp

# AatII.SmlI (GACGTC+CTYRAG) double digest, 2798 thousand fragments (71089+2727867 sites), 1064 bp average fragment length
   5% of sequence in fragments shorter than     364 bp
  10% of sequence in fragments shorter than     559 bp
  15% of sequence in fragments shorter than     723 bp
  20% of sequence in fragments shorter than     880 bp
  25% of sequence in fragments shorter than    1031 bp
  30% of sequence in fragments shorter than    1184 bp
  35% of sequence in fragments shorter than    1340 bp
  40% of sequence in fragments shorter than    1499 bp
  45% of sequence in fragments shorter than    1671 bp
  50% of sequence in fragments shorter than    1851 bp
  55% of sequence in fragments shorter than    2042 bp
  60% of sequence in fragments shorter than    2246 bp
  65% of sequence in fragments shorter than    2478 bp
  70% of sequence in fragments shorter than    2716 bp
  75% of sequence in fragments shorter than    3015 bp
  80% of sequence in fragments shorter than    3385 bp
  85% of sequence in fragments shorter than    3854 bp
  90% of sequence in fragments shorter than    4531 bp
  95% of sequence in fragments shorter than    5800 bp

# SacII.SmlI (CCGCGG+CTYRAG) double digest, 2794 thousand fragments (66901+2727867 sites), 1066 bp average fragment length
   5% of sequence in fragments shorter than     363 bp
  10% of sequence in fragments shorter than     560 bp
  15% of sequence in fragments shorter than     725 bp
  20% of sequence in fragments shorter than     884 bp
  25% of sequence in fragments shorter than    1035 bp
  30% of sequence in fragments shorter than    1190 bp
  35% of sequence in fragments shorter than    1346 bp
  40% of sequence in fragments shorter than    1508 bp
  45% of sequence in fragments shorter than    1682 bp
  50% of sequence in fragments shorter than    1864 bp
  55% of sequence in fragments shorter than    2057 bp
  60% of sequence in fragments shorter than    2264 bp
  65% of sequence in fragments shorter than    2499 bp
  70% of sequence in fragments shorter than    2740 bp
  75% of sequence in fragments shorter than    3043 bp
  80% of sequence in fragments shorter than    3418 bp
  85% of sequence in fragments shorter than    3894 bp
  90% of sequence in fragments shorter than    4583 bp
  95% of sequence in fragments shorter than    5872 bp

# BfiI.PpuMI (ACTGGG+RGGWCCY) double digest, 2791 thousand fragments (1706594+1085138 sites), 1067 bp average fragment length
   5% of sequence in fragments shorter than     347 bp
  10% of sequence in fragments shorter than     557 bp
  15% of sequence in fragments shorter than     743 bp
  20% of sequence in fragments shorter than     925 bp
  25% of sequence in fragments shorter than    1101 bp
  30% of sequence in fragments shorter than    1270 bp
  35% of sequence in fragments shorter than    1459 bp
  40% of sequence in fragments shorter than    1641 bp
  45% of sequence in fragments shorter than    1848 bp
  50% of sequence in fragments shorter than    2060 bp
  55% of sequence in fragments shorter than    2297 bp
  60% of sequence in fragments shorter than    2567 bp
  65% of sequence in fragments shorter than    2865 bp
  70% of sequence in fragments shorter than    3209 bp
  75% of sequence in fragments shorter than    3611 bp
  80% of sequence in fragments shorter than    4103 bp
  85% of sequence in fragments shorter than    4752 bp
  90% of sequence in fragments shorter than    5701 bp
  95% of sequence in fragments shorter than    7538 bp

# BfiI.PvuII (ACTGGG+CAGCTG) double digest, 2790 thousand fragments (1706594+1084260 sites), 1067 bp average fragment length
   5% of sequence in fragments shorter than     351 bp
  10% of sequence in fragments shorter than     554 bp
  15% of sequence in fragments shorter than     730 bp
  20% of sequence in fragments shorter than     900 bp
  25% of sequence in fragments shorter than    1065 bp
  30% of sequence in fragments shorter than    1236 bp
  35% of sequence in fragments shorter than    1411 bp
  40% of sequence in fragments shorter than    1597 bp
  45% of sequence in fragments shorter than    1790 bp
  50% of sequence in fragments shorter than    1998 bp
  55% of sequence in fragments shorter than    2210 bp
  60% of sequence in fragments shorter than    2457 bp
  65% of sequence in fragments shorter than    2737 bp
  70% of sequence in fragments shorter than    3022 bp
  75% of sequence in fragments shorter than    3400 bp
  80% of sequence in fragments shorter than    3861 bp
  85% of sequence in fragments shorter than    4454 bp
  90% of sequence in fragments shorter than    5304 bp
  95% of sequence in fragments shorter than    6929 bp

# AvaII.PmeI (GGWCC+GTTTAAAC) double digest, 2789 thousand fragments (2747690+42094 sites), 1068 bp average fragment length
   5% of sequence in fragments shorter than     343 bp
  10% of sequence in fragments shorter than     545 bp
  15% of sequence in fragments shorter than     738 bp
  20% of sequence in fragments shorter than     925 bp
  25% of sequence in fragments shorter than    1109 bp
  30% of sequence in fragments shorter than    1307 bp
  35% of sequence in fragments shorter than    1511 bp
  40% of sequence in fragments shorter than    1727 bp
  45% of sequence in fragments shorter than    1947 bp
  50% of sequence in fragments shorter than    2191 bp
  55% of sequence in fragments shorter than    2454 bp
  60% of sequence in fragments shorter than    2738 bp
  65% of sequence in fragments shorter than    3064 bp
  70% of sequence in fragments shorter than    3437 bp
  75% of sequence in fragments shorter than    3875 bp
  80% of sequence in fragments shorter than    4418 bp
  85% of sequence in fragments shorter than    5124 bp
  90% of sequence in fragments shorter than    6139 bp
  95% of sequence in fragments shorter than    8008 bp

# PspXI.SmlI (VCTCGAGB+CTYRAG) double digest, 2786 thousand fragments (59193+2727867 sites), 1069 bp average fragment length
   5% of sequence in fragments shorter than     374 bp
  10% of sequence in fragments shorter than     574 bp
  15% of sequence in fragments shorter than     742 bp
  20% of sequence in fragments shorter than     904 bp
  25% of sequence in fragments shorter than    1058 bp
  30% of sequence in fragments shorter than    1215 bp
  35% of sequence in fragments shorter than    1375 bp
  40% of sequence in fragments shorter than    1540 bp
  45% of sequence in fragments shorter than    1717 bp
  50% of sequence in fragments shorter than    1901 bp
  55% of sequence in fragments shorter than    2092 bp
  60% of sequence in fragments shorter than    2308 bp
  65% of sequence in fragments shorter than    2546 bp
  70% of sequence in fragments shorter than    2789 bp
  75% of sequence in fragments shorter than    3099 bp
  80% of sequence in fragments shorter than    3477 bp
  85% of sequence in fragments shorter than    3963 bp
  90% of sequence in fragments shorter than    4660 bp
  95% of sequence in fragments shorter than    5970 bp

# BscGI.HgiCI (CCCGT+GGYRCC) double digest, 2781 thousand fragments (1231070+1550877 sites), 1071 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     534 bp
  15% of sequence in fragments shorter than     733 bp
  20% of sequence in fragments shorter than     933 bp
  25% of sequence in fragments shorter than    1137 bp
  30% of sequence in fragments shorter than    1330 bp
  35% of sequence in fragments shorter than    1546 bp
  40% of sequence in fragments shorter than    1768 bp
  45% of sequence in fragments shorter than    2014 bp
  50% of sequence in fragments shorter than    2277 bp
  55% of sequence in fragments shorter than    2567 bp
  60% of sequence in fragments shorter than    2878 bp
  65% of sequence in fragments shorter than    3235 bp
  70% of sequence in fragments shorter than    3647 bp
  75% of sequence in fragments shorter than    4130 bp
  80% of sequence in fragments shorter than    4718 bp
  85% of sequence in fragments shorter than    5489 bp
  90% of sequence in fragments shorter than    6565 bp
  95% of sequence in fragments shorter than    8440 bp

# AgeI.SmlI (ACCGGT+CTYRAG) double digest, 2780 thousand fragments (53386+2727867 sites), 1071 bp average fragment length
   5% of sequence in fragments shorter than     365 bp
  10% of sequence in fragments shorter than     562 bp
  15% of sequence in fragments shorter than     727 bp
  20% of sequence in fragments shorter than     886 bp
  25% of sequence in fragments shorter than    1037 bp
  30% of sequence in fragments shorter than    1191 bp
  35% of sequence in fragments shorter than    1347 bp
  40% of sequence in fragments shorter than    1508 bp
  45% of sequence in fragments shorter than    1682 bp
  50% of sequence in fragments shorter than    1863 bp
  55% of sequence in fragments shorter than    2055 bp
  60% of sequence in fragments shorter than    2261 bp
  65% of sequence in fragments shorter than    2486 bp
  70% of sequence in fragments shorter than    2734 bp
  75% of sequence in fragments shorter than    3036 bp
  80% of sequence in fragments shorter than    3409 bp
  85% of sequence in fragments shorter than    3884 bp
  90% of sequence in fragments shorter than    4570 bp
  95% of sequence in fragments shorter than    5856 bp

# BscGI.BsmI (CCCGT+GAATGC) double digest, 2779 thousand fragments (1231070+1549350 sites), 1071 bp average fragment length
   5% of sequence in fragments shorter than     343 bp
  10% of sequence in fragments shorter than     582 bp
  15% of sequence in fragments shorter than     797 bp
  20% of sequence in fragments shorter than    1002 bp
  25% of sequence in fragments shorter than    1196 bp
  30% of sequence in fragments shorter than    1392 bp
  35% of sequence in fragments shorter than    1592 bp
  40% of sequence in fragments shorter than    1798 bp
  45% of sequence in fragments shorter than    2012 bp
  50% of sequence in fragments shorter than    2239 bp
  55% of sequence in fragments shorter than    2480 bp
  60% of sequence in fragments shorter than    2743 bp
  65% of sequence in fragments shorter than    3029 bp
  70% of sequence in fragments shorter than    3363 bp
  75% of sequence in fragments shorter than    3744 bp
  80% of sequence in fragments shorter than    4202 bp
  85% of sequence in fragments shorter than    4781 bp
  90% of sequence in fragments shorter than    5592 bp
  95% of sequence in fragments shorter than    6953 bp

# AvaII.SalI (GGWCC+GTCGAC) double digest, 2778 thousand fragments (2747690+31396 sites), 1072 bp average fragment length
   5% of sequence in fragments shorter than     343 bp
  10% of sequence in fragments shorter than     546 bp
  15% of sequence in fragments shorter than     742 bp
  20% of sequence in fragments shorter than     929 bp
  25% of sequence in fragments shorter than    1116 bp
  30% of sequence in fragments shorter than    1318 bp
  35% of sequence in fragments shorter than    1524 bp
  40% of sequence in fragments shorter than    1740 bp
  45% of sequence in fragments shorter than    1967 bp
  50% of sequence in fragments shorter than    2216 bp
  55% of sequence in fragments shorter than    2483 bp
  60% of sequence in fragments shorter than    2771 bp
  65% of sequence in fragments shorter than    3102 bp
  70% of sequence in fragments shorter than    3481 bp
  75% of sequence in fragments shorter than    3931 bp
  80% of sequence in fragments shorter than    4486 bp
  85% of sequence in fragments shorter than    5202 bp
  90% of sequence in fragments shorter than    6242 bp
  95% of sequence in fragments shorter than    8144 bp

# ApaI.HpaII (GGGCCC+CCGG) double digest, 2777 thousand fragments (460339+2317722 sites), 1072 bp average fragment length
   5% of sequence in fragments shorter than     325 bp
  10% of sequence in fragments shorter than     597 bp
  15% of sequence in fragments shorter than     858 bp
  20% of sequence in fragments shorter than    1123 bp
  25% of sequence in fragments shorter than    1397 bp
  30% of sequence in fragments shorter than    1685 bp
  35% of sequence in fragments shorter than    1997 bp
  40% of sequence in fragments shorter than    2330 bp
  45% of sequence in fragments shorter than    2699 bp
  50% of sequence in fragments shorter than    3100 bp
  55% of sequence in fragments shorter than    3549 bp
  60% of sequence in fragments shorter than    4047 bp
  65% of sequence in fragments shorter than    4624 bp
  70% of sequence in fragments shorter than    5295 bp
  75% of sequence in fragments shorter than    6082 bp
  80% of sequence in fragments shorter than    7066 bp
  85% of sequence in fragments shorter than    8371 bp
  90% of sequence in fragments shorter than   10310 bp
  95% of sequence in fragments shorter than   14126 bp

# SpeI.SspI (ACTAGT+AATATT) double digest, 2777 thousand fragments (400286+2377267 sites), 1073 bp average fragment length
   5% of sequence in fragments shorter than     350 bp
  10% of sequence in fragments shorter than     554 bp
  15% of sequence in fragments shorter than     730 bp
  20% of sequence in fragments shorter than     903 bp
  25% of sequence in fragments shorter than    1071 bp
  30% of sequence in fragments shorter than    1251 bp
  35% of sequence in fragments shorter than    1430 bp
  40% of sequence in fragments shorter than    1626 bp
  45% of sequence in fragments shorter than    1838 bp
  50% of sequence in fragments shorter than    2066 bp
  55% of sequence in fragments shorter than    2322 bp
  60% of sequence in fragments shorter than    2613 bp
  65% of sequence in fragments shorter than    2953 bp
  70% of sequence in fragments shorter than    3348 bp
  75% of sequence in fragments shorter than    3805 bp
  80% of sequence in fragments shorter than    4440 bp
  85% of sequence in fragments shorter than    5355 bp
  90% of sequence in fragments shorter than    6868 bp
  95% of sequence in fragments shorter than   10528 bp

# PmeI.SmlI (GTTTAAAC+CTYRAG) double digest, 2769 thousand fragments (42094+2727867 sites), 1076 bp average fragment length
   5% of sequence in fragments shorter than     368 bp
  10% of sequence in fragments shorter than     565 bp
  15% of sequence in fragments shorter than     730 bp
  20% of sequence in fragments shorter than     890 bp
  25% of sequence in fragments shorter than    1041 bp
  30% of sequence in fragments shorter than    1196 bp
  35% of sequence in fragments shorter than    1353 bp
  40% of sequence in fragments shorter than    1515 bp
  45% of sequence in fragments shorter than    1688 bp
  50% of sequence in fragments shorter than    1870 bp
  55% of sequence in fragments shorter than    2062 bp
  60% of sequence in fragments shorter than    2269 bp
  65% of sequence in fragments shorter than    2503 bp
  70% of sequence in fragments shorter than    2743 bp
  75% of sequence in fragments shorter than    3044 bp
  80% of sequence in fragments shorter than    3418 bp
  85% of sequence in fragments shorter than    3892 bp
  90% of sequence in fragments shorter than    4575 bp
  95% of sequence in fragments shorter than    5858 bp

# AvaII.MluI (GGWCC+ACGCGT) double digest, 2768 thousand fragments (2747690+21415 sites), 1076 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     548 bp
  15% of sequence in fragments shorter than     744 bp
  20% of sequence in fragments shorter than     931 bp
  25% of sequence in fragments shorter than    1121 bp
  30% of sequence in fragments shorter than    1324 bp
  35% of sequence in fragments shorter than    1531 bp
  40% of sequence in fragments shorter than    1747 bp
  45% of sequence in fragments shorter than    1976 bp
  50% of sequence in fragments shorter than    2226 bp
  55% of sequence in fragments shorter than    2496 bp
  60% of sequence in fragments shorter than    2785 bp
  65% of sequence in fragments shorter than    3119 bp
  70% of sequence in fragments shorter than    3502 bp
  75% of sequence in fragments shorter than    3956 bp
  80% of sequence in fragments shorter than    4513 bp
  85% of sequence in fragments shorter than    5236 bp
  90% of sequence in fragments shorter than    6287 bp
  95% of sequence in fragments shorter than    8200 bp

# HpaI.SspI (GTTAAC+AATATT) double digest, 2766 thousand fragments (389418+2377267 sites), 1077 bp average fragment length
   5% of sequence in fragments shorter than     350 bp
  10% of sequence in fragments shorter than     552 bp
  15% of sequence in fragments shorter than     731 bp
  20% of sequence in fragments shorter than     907 bp
  25% of sequence in fragments shorter than    1082 bp
  30% of sequence in fragments shorter than    1265 bp
  35% of sequence in fragments shorter than    1448 bp
  40% of sequence in fragments shorter than    1650 bp
  45% of sequence in fragments shorter than    1867 bp
  50% of sequence in fragments shorter than    2102 bp
  55% of sequence in fragments shorter than    2366 bp
  60% of sequence in fragments shorter than    2664 bp
  65% of sequence in fragments shorter than    3007 bp
  70% of sequence in fragments shorter than    3415 bp
  75% of sequence in fragments shorter than    3910 bp
  80% of sequence in fragments shorter than    4538 bp
  85% of sequence in fragments shorter than    5430 bp
  90% of sequence in fragments shorter than    6922 bp
  95% of sequence in fragments shorter than   10547 bp

# EcoRV.HpaII (GATATC+CCGG) double digest, 2763 thousand fragments (446473+2317722 sites), 1078 bp average fragment length
   5% of sequence in fragments shorter than     347 bp
  10% of sequence in fragments shorter than     602 bp
  15% of sequence in fragments shorter than     832 bp
  20% of sequence in fragments shorter than    1058 bp
  25% of sequence in fragments shorter than    1289 bp
  30% of sequence in fragments shorter than    1521 bp
  35% of sequence in fragments shorter than    1761 bp
  40% of sequence in fragments shorter than    2016 bp
  45% of sequence in fragments shorter than    2287 bp
  50% of sequence in fragments shorter than    2584 bp
  55% of sequence in fragments shorter than    2901 bp
  60% of sequence in fragments shorter than    3258 bp
  65% of sequence in fragments shorter than    3660 bp
  70% of sequence in fragments shorter than    4116 bp
  75% of sequence in fragments shorter than    4652 bp
  80% of sequence in fragments shorter than    5317 bp
  85% of sequence in fragments shorter than    6163 bp
  90% of sequence in fragments shorter than    7390 bp
  95% of sequence in fragments shorter than    9673 bp

# AvaII.SgrAI (GGWCC+CRCCGGYG) double digest, 2762 thousand fragments (2747690+15270 sites), 1078 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     550 bp
  15% of sequence in fragments shorter than     748 bp
  20% of sequence in fragments shorter than     933 bp
  25% of sequence in fragments shorter than    1126 bp
  30% of sequence in fragments shorter than    1330 bp
  35% of sequence in fragments shorter than    1538 bp
  40% of sequence in fragments shorter than    1755 bp
  45% of sequence in fragments shorter than    1985 bp
  50% of sequence in fragments shorter than    2236 bp
  55% of sequence in fragments shorter than    2507 bp
  60% of sequence in fragments shorter than    2798 bp
  65% of sequence in fragments shorter than    3133 bp
  70% of sequence in fragments shorter than    3517 bp
  75% of sequence in fragments shorter than    3974 bp
  80% of sequence in fragments shorter than    4533 bp
  85% of sequence in fragments shorter than    5260 bp
  90% of sequence in fragments shorter than    6316 bp
  95% of sequence in fragments shorter than    8246 bp

# AvaII.NruI (GGWCC+TCGCGA) double digest, 2762 thousand fragments (2747690+14872 sites), 1078 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     550 bp
  15% of sequence in fragments shorter than     747 bp
  20% of sequence in fragments shorter than     932 bp
  25% of sequence in fragments shorter than    1124 bp
  30% of sequence in fragments shorter than    1327 bp
  35% of sequence in fragments shorter than    1534 bp
  40% of sequence in fragments shorter than    1751 bp
  45% of sequence in fragments shorter than    1980 bp
  50% of sequence in fragments shorter than    2230 bp
  55% of sequence in fragments shorter than    2500 bp
  60% of sequence in fragments shorter than    2790 bp
  65% of sequence in fragments shorter than    3124 bp
  70% of sequence in fragments shorter than    3507 bp
  75% of sequence in fragments shorter than    3961 bp
  80% of sequence in fragments shorter than    4520 bp
  85% of sequence in fragments shorter than    5243 bp
  90% of sequence in fragments shorter than    6294 bp
  95% of sequence in fragments shorter than    8217 bp

# AvaI.HhaI (CYCGRG+GCGC) double digest, 2761 thousand fragments (1005714+1756502 sites), 1079 bp average fragment length
   5% of sequence in fragments shorter than     346 bp
  10% of sequence in fragments shorter than     647 bp
  15% of sequence in fragments shorter than     905 bp
  20% of sequence in fragments shorter than    1187 bp
  25% of sequence in fragments shorter than    1483 bp
  30% of sequence in fragments shorter than    1794 bp
  35% of sequence in fragments shorter than    2138 bp
  40% of sequence in fragments shorter than    2514 bp
  45% of sequence in fragments shorter than    2923 bp
  50% of sequence in fragments shorter than    3376 bp
  55% of sequence in fragments shorter than    3879 bp
  60% of sequence in fragments shorter than    4431 bp
  65% of sequence in fragments shorter than    5070 bp
  70% of sequence in fragments shorter than    5804 bp
  75% of sequence in fragments shorter than    6672 bp
  80% of sequence in fragments shorter than    7748 bp
  85% of sequence in fragments shorter than    9126 bp
  90% of sequence in fragments shorter than   11095 bp
  95% of sequence in fragments shorter than   14564 bp

# AvaII.PvuI (GGWCC+CGATCG) double digest, 2761 thousand fragments (2747690+14186 sites), 1079 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     550 bp
  15% of sequence in fragments shorter than     747 bp
  20% of sequence in fragments shorter than     932 bp
  25% of sequence in fragments shorter than    1124 bp
  30% of sequence in fragments shorter than    1327 bp
  35% of sequence in fragments shorter than    1535 bp
  40% of sequence in fragments shorter than    1751 bp
  45% of sequence in fragments shorter than    1978 bp
  50% of sequence in fragments shorter than    2228 bp
  55% of sequence in fragments shorter than    2498 bp
  60% of sequence in fragments shorter than    2787 bp
  65% of sequence in fragments shorter than    3121 bp
  70% of sequence in fragments shorter than    3503 bp
  75% of sequence in fragments shorter than    3955 bp
  80% of sequence in fragments shorter than    4511 bp
  85% of sequence in fragments shorter than    5231 bp
  90% of sequence in fragments shorter than    6270 bp
  95% of sequence in fragments shorter than    8155 bp

# AvaII.FseI (GGWCC+GGCCGGCC) double digest, 2760 thousand fragments (2747690+13662 sites), 1079 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     550 bp
  15% of sequence in fragments shorter than     748 bp
  20% of sequence in fragments shorter than     933 bp
  25% of sequence in fragments shorter than    1126 bp
  30% of sequence in fragments shorter than    1330 bp
  35% of sequence in fragments shorter than    1538 bp
  40% of sequence in fragments shorter than    1756 bp
  45% of sequence in fragments shorter than    1985 bp
  50% of sequence in fragments shorter than    2236 bp
  55% of sequence in fragments shorter than    2508 bp
  60% of sequence in fragments shorter than    2798 bp
  65% of sequence in fragments shorter than    3134 bp
  70% of sequence in fragments shorter than    3518 bp
  75% of sequence in fragments shorter than    3974 bp
  80% of sequence in fragments shorter than    4534 bp
  85% of sequence in fragments shorter than    5261 bp
  90% of sequence in fragments shorter than    6318 bp
  95% of sequence in fragments shorter than    8246 bp

# FauI.PstI (CCCGC+CTGCAG) double digest, 2760 thousand fragments (1439772+1321469 sites), 1079 bp average fragment length
   5% of sequence in fragments shorter than     348 bp
  10% of sequence in fragments shorter than     590 bp
  15% of sequence in fragments shorter than     807 bp
  20% of sequence in fragments shorter than    1025 bp
  25% of sequence in fragments shorter than    1247 bp
  30% of sequence in fragments shorter than    1475 bp
  35% of sequence in fragments shorter than    1723 bp
  40% of sequence in fragments shorter than    1984 bp
  45% of sequence in fragments shorter than    2262 bp
  50% of sequence in fragments shorter than    2570 bp
  55% of sequence in fragments shorter than    2904 bp
  60% of sequence in fragments shorter than    3258 bp
  65% of sequence in fragments shorter than    3682 bp
  70% of sequence in fragments shorter than    4165 bp
  75% of sequence in fragments shorter than    4741 bp
  80% of sequence in fragments shorter than    5440 bp
  85% of sequence in fragments shorter than    6348 bp
  90% of sequence in fragments shorter than    7674 bp
  95% of sequence in fragments shorter than   10067 bp

# BspMI.PstI (ACCTGC+CTGCAG) double digest, 2759 thousand fragments (1438129+1321469 sites), 1080 bp average fragment length
   5% of sequence in fragments shorter than     370 bp
  10% of sequence in fragments shorter than     588 bp
  15% of sequence in fragments shorter than     774 bp
  20% of sequence in fragments shorter than     951 bp
  25% of sequence in fragments shorter than    1137 bp
  30% of sequence in fragments shorter than    1324 bp
  35% of sequence in fragments shorter than    1508 bp
  40% of sequence in fragments shorter than    1713 bp
  45% of sequence in fragments shorter than    1927 bp
  50% of sequence in fragments shorter than    2151 bp
  55% of sequence in fragments shorter than    2403 bp
  60% of sequence in fragments shorter than    2681 bp
  65% of sequence in fragments shorter than    2986 bp
  70% of sequence in fragments shorter than    3298 bp
  75% of sequence in fragments shorter than    3704 bp
  80% of sequence in fragments shorter than    4196 bp
  85% of sequence in fragments shorter than    4818 bp
  90% of sequence in fragments shorter than    5700 bp
  95% of sequence in fragments shorter than    7265 bp

# SalI.SmlI (GTCGAC+CTYRAG) double digest, 2758 thousand fragments (31396+2727867 sites), 1080 bp average fragment length
   5% of sequence in fragments shorter than     369 bp
  10% of sequence in fragments shorter than     568 bp
  15% of sequence in fragments shorter than     734 bp
  20% of sequence in fragments shorter than     894 bp
  25% of sequence in fragments shorter than    1046 bp
  30% of sequence in fragments shorter than    1201 bp
  35% of sequence in fragments shorter than    1359 bp
  40% of sequence in fragments shorter than    1522 bp
  45% of sequence in fragments shorter than    1697 bp
  50% of sequence in fragments shorter than    1879 bp
  55% of sequence in fragments shorter than    2069 bp
  60% of sequence in fragments shorter than    2281 bp
  65% of sequence in fragments shorter than    2517 bp
  70% of sequence in fragments shorter than    2757 bp
  75% of sequence in fragments shorter than    3063 bp
  80% of sequence in fragments shorter than    3438 bp
  85% of sequence in fragments shorter than    3915 bp
  90% of sequence in fragments shorter than    4607 bp
  95% of sequence in fragments shorter than    5898 bp

# AvaII.NotI (GGWCC+GCGGCCGC) double digest, 2757 thousand fragments (2747690+9795 sites), 1080 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     551 bp
  15% of sequence in fragments shorter than     749 bp
  20% of sequence in fragments shorter than     933 bp
  25% of sequence in fragments shorter than    1128 bp
  30% of sequence in fragments shorter than    1332 bp
  35% of sequence in fragments shorter than    1541 bp
  40% of sequence in fragments shorter than    1758 bp
  45% of sequence in fragments shorter than    1988 bp
  50% of sequence in fragments shorter than    2240 bp
  55% of sequence in fragments shorter than    2511 bp
  60% of sequence in fragments shorter than    2802 bp
  65% of sequence in fragments shorter than    3138 bp
  70% of sequence in fragments shorter than    3523 bp
  75% of sequence in fragments shorter than    3980 bp
  80% of sequence in fragments shorter than    4540 bp
  85% of sequence in fragments shorter than    5267 bp
  90% of sequence in fragments shorter than    6324 bp
  95% of sequence in fragments shorter than    8253 bp

# AvaII.RsrII (GGWCC+CGGWCCG) double digest, 2756 thousand fragments (2747690+9764 sites), 1080 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     553 bp
  15% of sequence in fragments shorter than     751 bp
  20% of sequence in fragments shorter than     933 bp
  25% of sequence in fragments shorter than    1130 bp
  30% of sequence in fragments shorter than    1334 bp
  35% of sequence in fragments shorter than    1542 bp
  40% of sequence in fragments shorter than    1760 bp
  45% of sequence in fragments shorter than    1990 bp
  50% of sequence in fragments shorter than    2242 bp
  55% of sequence in fragments shorter than    2514 bp
  60% of sequence in fragments shorter than    2804 bp
  65% of sequence in fragments shorter than    3140 bp
  70% of sequence in fragments shorter than    3525 bp
  75% of sequence in fragments shorter than    3983 bp
  80% of sequence in fragments shorter than    4543 bp
  85% of sequence in fragments shorter than    5270 bp
  90% of sequence in fragments shorter than    6328 bp
  95% of sequence in fragments shorter than    8258 bp

# SapI.SspI (GCTCTTC+AATATT) double digest, 2753 thousand fragments (377161+2377267 sites), 1082 bp average fragment length
   5% of sequence in fragments shorter than     355 bp
  10% of sequence in fragments shorter than     562 bp
  15% of sequence in fragments shorter than     740 bp
  20% of sequence in fragments shorter than     913 bp
  25% of sequence in fragments shorter than    1086 bp
  30% of sequence in fragments shorter than    1263 bp
  35% of sequence in fragments shorter than    1437 bp
  40% of sequence in fragments shorter than    1628 bp
  45% of sequence in fragments shorter than    1832 bp
  50% of sequence in fragments shorter than    2052 bp
  55% of sequence in fragments shorter than    2290 bp
  60% of sequence in fragments shorter than    2560 bp
  65% of sequence in fragments shorter than    2869 bp
  70% of sequence in fragments shorter than    3230 bp
  75% of sequence in fragments shorter than    3667 bp
  80% of sequence in fragments shorter than    4194 bp
  85% of sequence in fragments shorter than    4898 bp
  90% of sequence in fragments shorter than    6005 bp
  95% of sequence in fragments shorter than    8288 bp

# SmaI.SspI (CCCGGG+AATATT) double digest, 2753 thousand fragments (376940+2377267 sites), 1082 bp average fragment length
   5% of sequence in fragments shorter than     359 bp
  10% of sequence in fragments shorter than     562 bp
  15% of sequence in fragments shorter than     737 bp
  20% of sequence in fragments shorter than     909 bp
  25% of sequence in fragments shorter than    1079 bp
  30% of sequence in fragments shorter than    1252 bp
  35% of sequence in fragments shorter than    1426 bp
  40% of sequence in fragments shorter than    1610 bp
  45% of sequence in fragments shorter than    1807 bp
  50% of sequence in fragments shorter than    2017 bp
  55% of sequence in fragments shorter than    2247 bp
  60% of sequence in fragments shorter than    2500 bp
  65% of sequence in fragments shorter than    2788 bp
  70% of sequence in fragments shorter than    3121 bp
  75% of sequence in fragments shorter than    3518 bp
  80% of sequence in fragments shorter than    4003 bp
  85% of sequence in fragments shorter than    4605 bp
  90% of sequence in fragments shorter than    5501 bp
  95% of sequence in fragments shorter than    7268 bp

# AscI.AvaII (GGCGCGCC+GGWCC) double digest, 2751 thousand fragments (4751+2747690 sites), 1082 bp average fragment length
   5% of sequence in fragments shorter than     344 bp
  10% of sequence in fragments shorter than     552 bp
  15% of sequence in fragments shorter than     750 bp
  20% of sequence in fragments shorter than     933 bp
  25% of sequence in fragments shorter than    1129 bp
  30% of sequence in fragments shorter than    1333 bp
  35% of sequence in fragments shorter than    1541 bp
  40% of sequence in fragments shorter than    1759 bp
  45% of sequence in fragments shorter than    1989 bp
  50% of sequence in fragments shorter than    2240 bp
  55% of sequence in fragments shorter than    2512 bp
  60% of sequence in fragments shorter than    2803 bp
  65% of sequence in fragments shorter than    3139 bp
  70% of sequence in fragments shorter than    3524 bp
  75% of sequence in fragments shorter than    3981 bp
  80% of sequence in fragments shorter than    4541 bp
  85% of sequence in fragments shorter than    5267 bp
  90% of sequence in fragments shorter than    6324 bp
  95% of sequence in fragments shorter than    8255 bp

# MluI.SmlI (ACGCGT+CTYRAG) double digest, 2748 thousand fragments (21415+2727867 sites), 1084 bp average fragment length
   5% of sequence in fragments shorter than     371 bp
  10% of sequence in fragments shorter than     570 bp
  15% of sequence in fragments shorter than     736 bp
  20% of sequence in fragments shorter than     897 bp
  25% of sequence in fragments shorter than    1049 bp
  30% of sequence in fragments shorter than    1206 bp
  35% of sequence in fragments shorter than    1364 bp
  40% of sequence in fragments shorter than    1527 bp
  45% of sequence in fragments shorter than    1703 bp
  50% of sequence in fragments shorter than    1885 bp
  55% of sequence in fragments shorter than    2075 bp
  60% of sequence in fragments shorter than    2289 bp
  65% of sequence in fragments shorter than    2525 bp
  70% of sequence in fragments shorter than    2766 bp
  75% of sequence in fragments shorter than    3073 bp
  80% of sequence in fragments shorter than    3448 bp
  85% of sequence in fragments shorter than    3929 bp
  90% of sequence in fragments shorter than    4620 bp
  95% of sequence in fragments shorter than    5913 bp

# AccI.PsiI (GTMKAC+TTATAA) double digest, 2748 thousand fragments (1101063+1647912 sites), 1084 bp average fragment length
   5% of sequence in fragments shorter than     368 bp
  10% of sequence in fragments shorter than     561 bp
  15% of sequence in fragments shorter than     738 bp
  20% of sequence in fragments shorter than     908 bp
  25% of sequence in fragments shorter than    1076 bp
  30% of sequence in fragments shorter than    1245 bp
  35% of sequence in fragments shorter than    1415 bp
  40% of sequence in fragments shorter than    1599 bp
  45% of sequence in fragments shorter than    1794 bp
  50% of sequence in fragments shorter than    2001 bp
  55% of sequence in fragments shorter than    2230 bp
  60% of sequence in fragments shorter than    2481 bp
  65% of sequence in fragments shorter than    2758 bp
  70% of sequence in fragments shorter than    3073 bp
  75% of sequence in fragments shorter than    3450 bp
  80% of sequence in fragments shorter than    3950 bp
  85% of sequence in fragments shorter than    4629 bp
  90% of sequence in fragments shorter than    5652 bp
  95% of sequence in fragments shorter than    7755 bp

# BamHI.SspI (GGATCC+AATATT) double digest, 2742 thousand fragments (365999+2377267 sites), 1086 bp average fragment length
   5% of sequence in fragments shorter than     363 bp
  10% of sequence in fragments shorter than     568 bp
  15% of sequence in fragments shorter than     747 bp
  20% of sequence in fragments shorter than     921 bp
  25% of sequence in fragments shorter than    1091 bp
  30% of sequence in fragments shorter than    1265 bp
  35% of sequence in fragments shorter than    1433 bp
  40% of sequence in fragments shorter than    1619 bp
  45% of sequence in fragments shorter than    1815 bp
  50% of sequence in fragments shorter than    2028 bp
  55% of sequence in fragments shorter than    2261 bp
  60% of sequence in fragments shorter than    2517 bp
  65% of sequence in fragments shorter than    2807 bp
  70% of sequence in fragments shorter than    3127 bp
  75% of sequence in fragments shorter than    3526 bp
  80% of sequence in fragments shorter than    4035 bp
  85% of sequence in fragments shorter than    4714 bp
  90% of sequence in fragments shorter than    5745 bp
  95% of sequence in fragments shorter than    7814 bp

# SgrAI.SmlI (CRCCGGYG+CTYRAG) double digest, 2742 thousand fragments (15270+2727867 sites), 1086 bp average fragment length
   5% of sequence in fragments shorter than     371 bp
  10% of sequence in fragments shorter than     571 bp
  15% of sequence in fragments shorter than     737 bp
  20% of sequence in fragments shorter than     899 bp
  25% of sequence in fragments shorter than    1052 bp
  30% of sequence in fragments shorter than    1208 bp
  35% of sequence in fragments shorter than    1367 bp
  40% of sequence in fragments shorter than    1531 bp
  45% of sequence in fragments shorter than    1706 bp
  50% of sequence in fragments shorter than    1890 bp
  55% of sequence in fragments shorter than    2080 bp
  60% of sequence in fragments shorter than    2294 bp
  65% of sequence in fragments shorter than    2531 bp
  70% of sequence in fragments shorter than    2773 bp
  75% of sequence in fragments shorter than    3081 bp
  80% of sequence in fragments shorter than    3457 bp
  85% of sequence in fragments shorter than    3939 bp
  90% of sequence in fragments shorter than    4634 bp
  95% of sequence in fragments shorter than    5935 bp

# NruI.SmlI (TCGCGA+CTYRAG) double digest, 2742 thousand fragments (14872+2727867 sites), 1086 bp average fragment length
   5% of sequence in fragments shorter than     372 bp
  10% of sequence in fragments shorter than     571 bp
  15% of sequence in fragments shorter than     738 bp
  20% of sequence in fragments shorter than     899 bp
  25% of sequence in fragments shorter than    1052 bp
  30% of sequence in fragments shorter than    1208 bp
  35% of sequence in fragments shorter than    1367 bp
  40% of sequence in fragments shorter than    1531 bp
  45% of sequence in fragments shorter than    1707 bp
  50% of sequence in fragments shorter than    1890 bp
  55% of sequence in fragments shorter than    2081 bp
  60% of sequence in fragments shorter than    2295 bp
  65% of sequence in fragments shorter than    2532 bp
  70% of sequence in fragments shorter than    2774 bp
  75% of sequence in fragments shorter than    3081 bp
  80% of sequence in fragments shorter than    3458 bp
  85% of sequence in fragments shorter than    3940 bp
  90% of sequence in fragments shorter than    4635 bp
  95% of sequence in fragments shorter than    5934 bp

# PvuI.SmlI (CGATCG+CTYRAG) double digest, 2741 thousand fragments (14186+2727867 sites), 1086 bp average fragment length
   5% of sequence in fragments shorter than     372 bp
  10% of sequence in fragments shorter than     571 bp
  15% of sequence in fragments shorter than     738 bp
  20% of sequence in fragments shorter than     900 bp
  25% of sequence in fragments shorter than    1053 bp
  30% of sequence in fragments shorter than    1210 bp
  35% of sequence in fragments shorter than    1368 bp
  40% of sequence in fragments shorter than    1532 bp
  45% of sequence in fragments shorter than    1708 bp
  50% of sequence in fragments shorter than    1891 bp
  55% of sequence in fragments shorter than    2079 bp
  60% of sequence in fragments shorter than    2293 bp
  65% of sequence in fragments shorter than    2529 bp
  70% of sequence in fragments shorter than    2770 bp
  75% of sequence in fragments shorter than    3077 bp
  80% of sequence in fragments shorter than    3452 bp
  85% of sequence in fragments shorter than    3932 bp
  90% of sequence in fragments shorter than    4621 bp
  95% of sequence in fragments shorter than    5899 bp

# FseI.SmlI (GGCCGGCC+CTYRAG) double digest, 2741 thousand fragments (13662+2727867 sites), 1087 bp average fragment length
   5% of sequence in fragments shorter than     372 bp
  10% of sequence in fragments shorter than     571 bp
  15% of sequence in fragments shorter than     738 bp
  20% of sequence in fragments shorter than     900 bp
  25% of sequence in fragments shorter than    1053 bp
  30% of sequence in fragments shorter than    1209 bp
  35% of sequence in fragments shorter than    1368 bp
  40% of sequence in fragments shorter than    1532 bp
  45% of sequence in fragments shorter than    1708 bp
  50% of sequence in fragments shorter than    1891 bp
  55% of sequence in fragments shorter than    2081 bp
  60% of sequence in fragments shorter than    2296 bp
  65% of sequence in fragments shorter than    2533 bp
  70% of sequence in fragments shorter than    2775 bp
  75% of sequence in fragments shorter than    3082 bp
  80% of sequence in fragments shorter than    3458 bp
  85% of sequence in fragments shorter than    3941 bp
  90% of sequence in fragments shorter than    4636 bp
  95% of sequence in fragments shorter than    5937 bp

# NotI.SmlI (GCGGCCGC+CTYRAG) double digest, 2737 thousand fragments (9795+2727867 sites), 1088 bp average fragment length
   5% of sequence in fragments shorter than     372 bp
  10% of sequence in fragments shorter than     572 bp
  15% of sequence in fragments shorter than     739 bp
  20% of sequence in fragments shorter than     901 bp
  25% of sequence in fragments shorter than    1054 bp
  30% of sequence in fragments shorter than    1211 bp
  35% of sequence in fragments shorter than    1369 bp
  40% of sequence in fragments shorter than    1534 bp
  45% of sequence in fragments shorter than    1710 bp
  50% of sequence in fragments shorter than    1894 bp
  55% of sequence in fragments shorter than    2084 bp
  60% of sequence in fragments shorter than    2298 bp
  65% of sequence in fragments shorter than    2536 bp
  70% of sequence in fragments shorter than    2778 bp
  75% of sequence in fragments shorter than    3086 bp
  80% of sequence in fragments shorter than    3464 bp
  85% of sequence in fragments shorter than    3947 bp
  90% of sequence in fragments shorter than    4643 bp
  95% of sequence in fragments shorter than    5945 bp

# RsrII.SmlI (CGGWCCG+CTYRAG) double digest, 2737 thousand fragments (9764+2727867 sites), 1088 bp average fragment length
   5% of sequence in fragments shorter than     372 bp
  10% of sequence in fragments shorter than     572 bp
  15% of sequence in fragments shorter than     739 bp
  20% of sequence in fragments shorter than     901 bp
  25% of sequence in fragments shorter than    1054 bp
  30% of sequence in fragments shorter than    1211 bp
  35% of sequence in fragments shorter than    1370 bp
  40% of sequence in fragments shorter than    1534 bp
  45% of sequence in fragments shorter than    1710 bp
  50% of sequence in fragments shorter than    1894 bp
  55% of sequence in fragments shorter than    2084 bp
  60% of sequence in fragments shorter than    2298 bp
  65% of sequence in fragments shorter than    2535 bp
  70% of sequence in fragments shorter than    2778 bp
  75% of sequence in fragments shorter than    3086 bp
  80% of sequence in fragments shorter than    3464 bp
  85% of sequence in fragments shorter than    3946 bp
  90% of sequence in fragments shorter than    4639 bp
  95% of sequence in fragments shorter than    5940 bp

# BspHI.HhaI (TCATGA+GCGC) double digest, 2734 thousand fragments (978290+1756502 sites), 1089 bp average fragment length
   5% of sequence in fragments shorter than     371 bp
  10% of sequence in fragments shorter than     631 bp
  15% of sequence in fragments shorter than     835 bp
  20% of sequence in fragments shorter than    1038 bp
  25% of sequence in fragments shorter than    1248 bp
  30% of sequence in fragments shorter than    1452 bp
  35% of sequence in fragments shorter than    1666 bp
  40% of sequence in fragments shorter than    1881 bp
  45% of sequence in fragments shorter than    2107 bp
  50% of sequence in fragments shorter than    2350 bp
  55% of sequence in fragments shorter than    2608 bp
  60% of sequence in fragments shorter than    2897 bp
  65% of sequence in fragments shorter than    3212 bp
  70% of sequence in fragments shorter than    3565 bp
  75% of sequence in fragments shorter than    3976 bp
  80% of sequence in fragments shorter than    4460 bp
  85% of sequence in fragments shorter than    5099 bp
  90% of sequence in fragments shorter than    5980 bp
  95% of sequence in fragments shorter than    7481 bp

# PpuMI.PsiI (RGGWCCY+TTATAA) double digest, 2732 thousand fragments (1085138+1647912 sites), 1090 bp average fragment length
   5% of sequence in fragments shorter than     375 bp
  10% of sequence in fragments shorter than     580 bp
  15% of sequence in fragments shorter than     756 bp
  20% of sequence in fragments shorter than     923 bp
  25% of sequence in fragments shorter than    1084 bp
  30% of sequence in fragments shorter than    1239 bp
  35% of sequence in fragments shorter than    1403 bp
  40% of sequence in fragments shorter than    1574 bp
  45% of sequence in fragments shorter than    1742 bp
  50% of sequence in fragments shorter than    1923 bp
  55% of sequence in fragments shorter than    2123 bp
  60% of sequence in fragments shorter than    2346 bp
  65% of sequence in fragments shorter than    2594 bp
  70% of sequence in fragments shorter than    2878 bp
  75% of sequence in fragments shorter than    3202 bp
  80% of sequence in fragments shorter than    3601 bp
  85% of sequence in fragments shorter than    4116 bp
  90% of sequence in fragments shorter than    4893 bp
  95% of sequence in fragments shorter than    6454 bp

# AscI.SmlI (GGCGCGCC+CTYRAG) double digest, 2732 thousand fragments (4751+2727867 sites), 1090 bp average fragment length
   5% of sequence in fragments shorter than     373 bp
  10% of sequence in fragments shorter than     573 bp
  15% of sequence in fragments shorter than     740 bp
  20% of sequence in fragments shorter than     903 bp
  25% of sequence in fragments shorter than    1056 bp
  30% of sequence in fragments shorter than    1213 bp
  35% of sequence in fragments shorter than    1372 bp
  40% of sequence in fragments shorter than    1537 bp
  45% of sequence in fragments shorter than    1713 bp
  50% of sequence in fragments shorter than    1897 bp
  55% of sequence in fragments shorter than    2088 bp
  60% of sequence in fragments shorter than    2303 bp
  65% of sequence in fragments shorter than    2541 bp
  70% of sequence in fragments shorter than    2783 bp
  75% of sequence in fragments shorter than    3092 bp
  80% of sequence in fragments shorter than    3470 bp
  85% of sequence in fragments shorter than    3954 bp
  90% of sequence in fragments shorter than    4651 bp
  95% of sequence in fragments shorter than    5958 bp

# PsiI.PvuII (TTATAA+CAGCTG) double digest, 2731 thousand fragments (1647912+1084260 sites), 1090 bp average fragment length
   5% of sequence in fragments shorter than     378 bp
  10% of sequence in fragments shorter than     579 bp
  15% of sequence in fragments shorter than     750 bp
  20% of sequence in fragments shorter than     910 bp
  25% of sequence in fragments shorter than    1066 bp
  30% of sequence in fragments shorter than    1223 bp
  35% of sequence in fragments shorter than    1384 bp
  40% of sequence in fragments shorter than    1552 bp
  45% of sequence in fragments shorter than    1728 bp
  50% of sequence in fragments shorter than    1908 bp
  55% of sequence in fragments shorter than    2109 bp
  60% of sequence in fragments shorter than    2325 bp
  65% of sequence in fragments shorter than    2570 bp
  70% of sequence in fragments shorter than    2846 bp
  75% of sequence in fragments shorter than    3162 bp
  80% of sequence in fragments shorter than    3558 bp
  85% of sequence in fragments shorter than    4070 bp
  90% of sequence in fragments shorter than    4820 bp
  95% of sequence in fragments shorter than    6281 bp

# SmlI.XhoI (CTYRAG+CTCGAG) double digest, 2727 thousand fragments (2727867+121308 sites), 1092 bp average fragment length
   5% of sequence in fragments shorter than     374 bp
  10% of sequence in fragments shorter than     574 bp
  15% of sequence in fragments shorter than     742 bp
  20% of sequence in fragments shorter than     904 bp
  25% of sequence in fragments shorter than    1058 bp
  30% of sequence in fragments shorter than    1215 bp
  35% of sequence in fragments shorter than    1375 bp
  40% of sequence in fragments shorter than    1540 bp
  45% of sequence in fragments shorter than    1717 bp
  50% of sequence in fragments shorter than    1901 bp
  55% of sequence in fragments shorter than    2092 bp
  60% of sequence in fragments shorter than    2308 bp
  65% of sequence in fragments shorter than    2546 bp
  70% of sequence in fragments shorter than    2789 bp
  75% of sequence in fragments shorter than    3099 bp
  80% of sequence in fragments shorter than    3477 bp
  85% of sequence in fragments shorter than    3963 bp
  90% of sequence in fragments shorter than    4660 bp
  95% of sequence in fragments shorter than    5970 bp

# AflII.SmlI (CTTAAG+CTYRAG) double digest, 2727 thousand fragments (643284+2727867 sites), 1092 bp average fragment length
   5% of sequence in fragments shorter than     374 bp
  10% of sequence in fragments shorter than     574 bp
  15% of sequence in fragments shorter than     742 bp
  20% of sequence in fragments shorter than     904 bp
  25% of sequence in fragments shorter than    1058 bp
  30% of sequence in fragments shorter than    1215 bp
  35% of sequence in fragments shorter than    1375 bp
  40% of sequence in fragments shorter than    1540 bp
  45% of sequence in fragments shorter than    1717 bp
  50% of sequence in fragments shorter than    1901 bp
  55% of sequence in fragments shorter than    2092 bp
  60% of sequence in fragments shorter than    2308 bp
  65% of sequence in fragments shorter than    2546 bp
  70% of sequence in fragments shorter than    2789 bp
  75% of sequence in fragments shorter than    3099 bp
  80% of sequence in fragments shorter than    3477 bp
  85% of sequence in fragments shorter than    3963 bp
  90% of sequence in fragments shorter than    4660 bp
  95% of sequence in fragments shorter than    5970 bp

# HpaII.SpeI (CCGG+ACTAGT) double digest, 2717 thousand fragments (2317722+400286 sites), 1096 bp average fragment length
   5% of sequence in fragments shorter than     350 bp
  10% of sequence in fragments shorter than     612 bp
  15% of sequence in fragments shorter than     851 bp
  20% of sequence in fragments shorter than    1084 bp
  25% of sequence in fragments shorter than    1320 bp
  30% of sequence in fragments shorter than    1560 bp
  35% of sequence in fragments shorter than    1807 bp
  40% of sequence in fragments shorter than    2067 bp
  45% of sequence in fragments shorter than    2352 bp
  50% of sequence in fragments shorter than    2661 bp
  55% of sequence in fragments shorter than    2997 bp
  60% of sequence in fragments shorter than    3372 bp
  65% of sequence in fragments shorter than    3782 bp
  70% of sequence in fragments shorter than    4264 bp
  75% of sequence in fragments shorter than    4836 bp
  80% of sequence in fragments shorter than    5524 bp
  85% of sequence in fragments shorter than    6425 bp
  90% of sequence in fragments shorter than    7752 bp
  95% of sequence in fragments shorter than   10381 bp

# AflIII.BscGI (ACRYGT+CCCGT) double digest, 2715 thousand fragments (1485394+1231070 sites), 1097 bp average fragment length
   5% of sequence in fragments shorter than     347 bp
  10% of sequence in fragments shorter than     560 bp
  15% of sequence in fragments shorter than     753 bp
  20% of sequence in fragments shorter than     936 bp
  25% of sequence in fragments shorter than    1116 bp
  30% of sequence in fragments shorter than    1297 bp
  35% of sequence in fragments shorter than    1481 bp
  40% of sequence in fragments shorter than    1673 bp
  45% of sequence in fragments shorter than    1872 bp
  50% of sequence in fragments shorter than    2083 bp
  55% of sequence in fragments shorter than    2308 bp
  60% of sequence in fragments shorter than    2554 bp
  65% of sequence in fragments shorter than    2828 bp
  70% of sequence in fragments shorter than    3135 bp
  75% of sequence in fragments shorter than    3490 bp
  80% of sequence in fragments shorter than    3915 bp
  85% of sequence in fragments shorter than    4447 bp
  90% of sequence in fragments shorter than    5151 bp
  95% of sequence in fragments shorter than    6388 bp

# BstEII.SspI (GGTNACC+AATATT) double digest, 2712 thousand fragments (335865+2377267 sites), 1098 bp average fragment length
   5% of sequence in fragments shorter than     365 bp
  10% of sequence in fragments shorter than     573 bp
  15% of sequence in fragments shorter than     755 bp
  20% of sequence in fragments shorter than     930 bp
  25% of sequence in fragments shorter than    1106 bp
  30% of sequence in fragments shorter than    1285 bp
  35% of sequence in fragments shorter than    1466 bp
  40% of sequence in fragments shorter than    1659 bp
  45% of sequence in fragments shorter than    1865 bp
  50% of sequence in fragments shorter than    2086 bp
  55% of sequence in fragments shorter than    2332 bp
  60% of sequence in fragments shorter than    2602 bp
  65% of sequence in fragments shorter than    2915 bp
  70% of sequence in fragments shorter than    3278 bp
  75% of sequence in fragments shorter than    3714 bp
  80% of sequence in fragments shorter than    4241 bp
  85% of sequence in fragments shorter than    4924 bp
  90% of sequence in fragments shorter than    5977 bp
  95% of sequence in fragments shorter than    8120 bp

# AvaI.BfiI (CYCGRG+ACTGGG) double digest, 2711 thousand fragments (1005714+1706594 sites), 1098 bp average fragment length
   5% of sequence in fragments shorter than     359 bp
  10% of sequence in fragments shorter than     568 bp
  15% of sequence in fragments shorter than     754 bp
  20% of sequence in fragments shorter than     934 bp
  25% of sequence in fragments shorter than    1109 bp
  30% of sequence in fragments shorter than    1291 bp
  35% of sequence in fragments shorter than    1483 bp
  40% of sequence in fragments shorter than    1684 bp
  45% of sequence in fragments shorter than    1895 bp
  50% of sequence in fragments shorter than    2125 bp
  55% of sequence in fragments shorter than    2374 bp
  60% of sequence in fragments shorter than    2655 bp
  65% of sequence in fragments shorter than    2939 bp
  70% of sequence in fragments shorter than    3299 bp
  75% of sequence in fragments shorter than    3730 bp
  80% of sequence in fragments shorter than    4248 bp
  85% of sequence in fragments shorter than    4936 bp
  90% of sequence in fragments shorter than    5938 bp
  95% of sequence in fragments shorter than    7948 bp

# HpaI.HpaII (GTTAAC+CCGG) double digest, 2706 thousand fragments (389418+2317722 sites), 1100 bp average fragment length
   5% of sequence in fragments shorter than     349 bp
  10% of sequence in fragments shorter than     612 bp
  15% of sequence in fragments shorter than     854 bp
  20% of sequence in fragments shorter than    1090 bp
  25% of sequence in fragments shorter than    1329 bp
  30% of sequence in fragments shorter than    1577 bp
  35% of sequence in fragments shorter than    1833 bp
  40% of sequence in fragments shorter than    2103 bp
  45% of sequence in fragments shorter than    2398 bp
  50% of sequence in fragments shorter than    2721 bp
  55% of sequence in fragments shorter than    3073 bp
  60% of sequence in fragments shorter than    3458 bp
  65% of sequence in fragments shorter than    3896 bp
  70% of sequence in fragments shorter than    4402 bp
  75% of sequence in fragments shorter than    5004 bp
  80% of sequence in fragments shorter than    5742 bp
  85% of sequence in fragments shorter than    6704 bp
  90% of sequence in fragments shorter than    8147 bp
  95% of sequence in fragments shorter than   10981 bp

# AccI.EsaBC3I (GTMKAC+TCGA) double digest, 2703 thousand fragments (1101063+1603346 sites), 1102 bp average fragment length
   5% of sequence in fragments shorter than     393 bp
  10% of sequence in fragments shorter than     587 bp
  15% of sequence in fragments shorter than     760 bp
  20% of sequence in fragments shorter than     922 bp
  25% of sequence in fragments shorter than    1078 bp
  30% of sequence in fragments shorter than    1235 bp
  35% of sequence in fragments shorter than    1390 bp
  40% of sequence in fragments shorter than    1558 bp
  45% of sequence in fragments shorter than    1731 bp
  50% of sequence in fragments shorter than    1914 bp
  55% of sequence in fragments shorter than    2111 bp
  60% of sequence in fragments shorter than    2323 bp
  65% of sequence in fragments shorter than    2556 bp
  70% of sequence in fragments shorter than    2815 bp
  75% of sequence in fragments shorter than    3122 bp
  80% of sequence in fragments shorter than    3495 bp
  85% of sequence in fragments shorter than    3965 bp
  90% of sequence in fragments shorter than    4606 bp
  95% of sequence in fragments shorter than    5735 bp

# HpaII.SapI (CCGG+GCTCTTC) double digest, 2694 thousand fragments (2317722+377161 sites), 1105 bp average fragment length
   5% of sequence in fragments shorter than     346 bp
  10% of sequence in fragments shorter than     612 bp
  15% of sequence in fragments shorter than     857 bp
  20% of sequence in fragments shorter than    1102 bp
  25% of sequence in fragments shorter than    1353 bp
  30% of sequence in fragments shorter than    1611 bp
  35% of sequence in fragments shorter than    1889 bp
  40% of sequence in fragments shorter than    2183 bp
  45% of sequence in fragments shorter than    2505 bp
  50% of sequence in fragments shorter than    2859 bp
  55% of sequence in fragments shorter than    3248 bp
  60% of sequence in fragments shorter than    3676 bp
  65% of sequence in fragments shorter than    4160 bp
  70% of sequence in fragments shorter than    4725 bp
  75% of sequence in fragments shorter than    5402 bp
  80% of sequence in fragments shorter than    6234 bp
  85% of sequence in fragments shorter than    7352 bp
  90% of sequence in fragments shorter than    8992 bp
  95% of sequence in fragments shorter than   12176 bp

# HpaII.SmaI (CCGG+CCCGGG) double digest, 2694 thousand fragments (2317722+376940 sites), 1106 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     727 bp
  15% of sequence in fragments shorter than    1040 bp
  20% of sequence in fragments shorter than    1359 bp
  25% of sequence in fragments shorter than    1683 bp
  30% of sequence in fragments shorter than    2034 bp
  35% of sequence in fragments shorter than    2406 bp
  40% of sequence in fragments shorter than    2817 bp
  45% of sequence in fragments shorter than    3263 bp
  50% of sequence in fragments shorter than    3751 bp
  55% of sequence in fragments shorter than    4291 bp
  60% of sequence in fragments shorter than    4906 bp
  65% of sequence in fragments shorter than    5602 bp
  70% of sequence in fragments shorter than    6398 bp
  75% of sequence in fragments shorter than    7370 bp
  80% of sequence in fragments shorter than    8590 bp
  85% of sequence in fragments shorter than   10188 bp
  90% of sequence in fragments shorter than   12542 bp
  95% of sequence in fragments shorter than   17302 bp

# AvaIII.HhaI (ATGCAT+GCGC) double digest, 2689 thousand fragments (933357+1756502 sites), 1108 bp average fragment length
   5% of sequence in fragments shorter than     377 bp
  10% of sequence in fragments shorter than     637 bp
  15% of sequence in fragments shorter than     846 bp
  20% of sequence in fragments shorter than    1050 bp
  25% of sequence in fragments shorter than    1262 bp
  30% of sequence in fragments shorter than    1469 bp
  35% of sequence in fragments shorter than    1687 bp
  40% of sequence in fragments shorter than    1912 bp
  45% of sequence in fragments shorter than    2150 bp
  50% of sequence in fragments shorter than    2406 bp
  55% of sequence in fragments shorter than    2680 bp
  60% of sequence in fragments shorter than    2981 bp
  65% of sequence in fragments shorter than    3317 bp
  70% of sequence in fragments shorter than    3702 bp
  75% of sequence in fragments shorter than    4144 bp
  80% of sequence in fragments shorter than    4688 bp
  85% of sequence in fragments shorter than    5365 bp
  90% of sequence in fragments shorter than    6310 bp
  95% of sequence in fragments shorter than    7944 bp

# EsaBC3I.PpuMI (TCGA+RGGWCCY) double digest, 2687 thousand fragments (1603346+1085138 sites), 1108 bp average fragment length
   5% of sequence in fragments shorter than     373 bp
  10% of sequence in fragments shorter than     571 bp
  15% of sequence in fragments shorter than     750 bp
  20% of sequence in fragments shorter than     922 bp
  25% of sequence in fragments shorter than    1090 bp
  30% of sequence in fragments shorter than    1267 bp
  35% of sequence in fragments shorter than    1439 bp
  40% of sequence in fragments shorter than    1629 bp
  45% of sequence in fragments shorter than    1828 bp
  50% of sequence in fragments shorter than    2040 bp
  55% of sequence in fragments shorter than    2268 bp
  60% of sequence in fragments shorter than    2516 bp
  65% of sequence in fragments shorter than    2797 bp
  70% of sequence in fragments shorter than    3110 bp
  75% of sequence in fragments shorter than    3485 bp
  80% of sequence in fragments shorter than    3938 bp
  85% of sequence in fragments shorter than    4524 bp
  90% of sequence in fragments shorter than    5363 bp
  95% of sequence in fragments shorter than    6831 bp

# EsaBC3I.PvuII (TCGA+CAGCTG) double digest, 2687 thousand fragments (1603346+1084260 sites), 1108 bp average fragment length
   5% of sequence in fragments shorter than     378 bp
  10% of sequence in fragments shorter than     573 bp
  15% of sequence in fragments shorter than     753 bp
  20% of sequence in fragments shorter than     921 bp
  25% of sequence in fragments shorter than    1088 bp
  30% of sequence in fragments shorter than    1258 bp
  35% of sequence in fragments shorter than    1428 bp
  40% of sequence in fragments shorter than    1612 bp
  45% of sequence in fragments shorter than    1807 bp
  50% of sequence in fragments shorter than    2014 bp
  55% of sequence in fragments shorter than    2241 bp
  60% of sequence in fragments shorter than    2489 bp
  65% of sequence in fragments shorter than    2765 bp
  70% of sequence in fragments shorter than    3082 bp
  75% of sequence in fragments shorter than    3450 bp
  80% of sequence in fragments shorter than    3899 bp
  85% of sequence in fragments shorter than    4477 bp
  90% of sequence in fragments shorter than    5304 bp
  95% of sequence in fragments shorter than    6736 bp

# BfiI.BspHI (ACTGGG+TCATGA) double digest, 2684 thousand fragments (1706594+978290 sites), 1110 bp average fragment length
   5% of sequence in fragments shorter than     381 bp
  10% of sequence in fragments shorter than     585 bp
  15% of sequence in fragments shorter than     759 bp
  20% of sequence in fragments shorter than     926 bp
  25% of sequence in fragments shorter than    1086 bp
  30% of sequence in fragments shorter than    1247 bp
  35% of sequence in fragments shorter than    1404 bp
  40% of sequence in fragments shorter than    1564 bp
  45% of sequence in fragments shorter than    1738 bp
  50% of sequence in fragments shorter than    1927 bp
  55% of sequence in fragments shorter than    2120 bp
  60% of sequence in fragments shorter than    2330 bp
  65% of sequence in fragments shorter than    2573 bp
  70% of sequence in fragments shorter than    2850 bp
  75% of sequence in fragments shorter than    3173 bp
  80% of sequence in fragments shorter than    3564 bp
  85% of sequence in fragments shorter than    4059 bp
  90% of sequence in fragments shorter than    4781 bp
  95% of sequence in fragments shorter than    6156 bp

# BamHI.HpaII (GGATCC+CCGG) double digest, 2683 thousand fragments (365999+2317722 sites), 1110 bp average fragment length
   5% of sequence in fragments shorter than     346 bp
  10% of sequence in fragments shorter than     619 bp
  15% of sequence in fragments shorter than     873 bp
  20% of sequence in fragments shorter than    1123 bp
  25% of sequence in fragments shorter than    1377 bp
  30% of sequence in fragments shorter than    1645 bp
  35% of sequence in fragments shorter than    1929 bp
  40% of sequence in fragments shorter than    2226 bp
  45% of sequence in fragments shorter than    2561 bp
  50% of sequence in fragments shorter than    2927 bp
  55% of sequence in fragments shorter than    3314 bp
  60% of sequence in fragments shorter than    3763 bp
  65% of sequence in fragments shorter than    4261 bp
  70% of sequence in fragments shorter than    4843 bp
  75% of sequence in fragments shorter than    5541 bp
  80% of sequence in fragments shorter than    6397 bp
  85% of sequence in fragments shorter than    7540 bp
  90% of sequence in fragments shorter than    9218 bp
  95% of sequence in fragments shorter than   12475 bp

# HhaI.StuI (GCGC+AGGCCT) double digest, 2681 thousand fragments (1756502+925729 sites), 1111 bp average fragment length
   5% of sequence in fragments shorter than     345 bp
  10% of sequence in fragments shorter than     611 bp
  15% of sequence in fragments shorter than     846 bp
  20% of sequence in fragments shorter than    1093 bp
  25% of sequence in fragments shorter than    1348 bp
  30% of sequence in fragments shorter than    1608 bp
  35% of sequence in fragments shorter than    1886 bp
  40% of sequence in fragments shorter than    2161 bp
  45% of sequence in fragments shorter than    2473 bp
  50% of sequence in fragments shorter than    2813 bp
  55% of sequence in fragments shorter than    3178 bp
  60% of sequence in fragments shorter than    3585 bp
  65% of sequence in fragments shorter than    4052 bp
  70% of sequence in fragments shorter than    4567 bp
  75% of sequence in fragments shorter than    5187 bp
  80% of sequence in fragments shorter than    5949 bp
  85% of sequence in fragments shorter than    6905 bp
  90% of sequence in fragments shorter than    8258 bp
  95% of sequence in fragments shorter than   10575 bp

# SexAI.SspI (ACCWGGT+AATATT) double digest, 2674 thousand fragments (297900+2377267 sites), 1114 bp average fragment length
   5% of sequence in fragments shorter than     369 bp
  10% of sequence in fragments shorter than     578 bp
  15% of sequence in fragments shorter than     759 bp
  20% of sequence in fragments shorter than     939 bp
  25% of sequence in fragments shorter than    1115 bp
  30% of sequence in fragments shorter than    1298 bp
  35% of sequence in fragments shorter than    1482 bp
  40% of sequence in fragments shorter than    1679 bp
  45% of sequence in fragments shorter than    1889 bp
  50% of sequence in fragments shorter than    2115 bp
  55% of sequence in fragments shorter than    2367 bp
  60% of sequence in fragments shorter than    2647 bp
  65% of sequence in fragments shorter than    2968 bp
  70% of sequence in fragments shorter than    3344 bp
  75% of sequence in fragments shorter than    3802 bp
  80% of sequence in fragments shorter than    4355 bp
  85% of sequence in fragments shorter than    5079 bp
  90% of sequence in fragments shorter than    6239 bp
  95% of sequence in fragments shorter than    8680 bp

# BscGI.FauI (CCCGT+CCCGC) double digest, 2670 thousand fragments (1231070+1439772 sites), 1115 bp average fragment length
   5% of sequence in fragments shorter than     345 bp
  10% of sequence in fragments shorter than     593 bp
  15% of sequence in fragments shorter than     864 bp
  20% of sequence in fragments shorter than    1142 bp
  25% of sequence in fragments shorter than    1429 bp
  30% of sequence in fragments shorter than    1734 bp
  35% of sequence in fragments shorter than    2056 bp
  40% of sequence in fragments shorter than    2402 bp
  45% of sequence in fragments shorter than    2774 bp
  50% of sequence in fragments shorter than    3180 bp
  55% of sequence in fragments shorter than    3618 bp
  60% of sequence in fragments shorter than    4111 bp
  65% of sequence in fragments shorter than    4669 bp
  70% of sequence in fragments shorter than    5322 bp
  75% of sequence in fragments shorter than    6088 bp
  80% of sequence in fragments shorter than    7023 bp
  85% of sequence in fragments shorter than    8242 bp
  90% of sequence in fragments shorter than    9970 bp
  95% of sequence in fragments shorter than   12996 bp

# BscGI.BspMI (CCCGT+ACCTGC) double digest, 2668 thousand fragments (1231070+1438129 sites), 1116 bp average fragment length
   5% of sequence in fragments shorter than     345 bp
  10% of sequence in fragments shorter than     558 bp
  15% of sequence in fragments shorter than     758 bp
  20% of sequence in fragments shorter than     958 bp
  25% of sequence in fragments shorter than    1159 bp
  30% of sequence in fragments shorter than    1362 bp
  35% of sequence in fragments shorter than    1573 bp
  40% of sequence in fragments shorter than    1794 bp
  45% of sequence in fragments shorter than    2024 bp
  50% of sequence in fragments shorter than    2277 bp
  55% of sequence in fragments shorter than    2554 bp
  60% of sequence in fragments shorter than    2858 bp
  65% of sequence in fragments shorter than    3202 bp
  70% of sequence in fragments shorter than    3545 bp
  75% of sequence in fragments shorter than    3997 bp
  80% of sequence in fragments shorter than    4541 bp
  85% of sequence in fragments shorter than    5232 bp
  90% of sequence in fragments shorter than    6205 bp
  95% of sequence in fragments shorter than    7896 bp

# KpnI.SspI (GGTACC+AATATT) double digest, 2664 thousand fragments (287974+2377267 sites), 1118 bp average fragment length
   5% of sequence in fragments shorter than     371 bp
  10% of sequence in fragments shorter than     584 bp
  15% of sequence in fragments shorter than     770 bp
  20% of sequence in fragments shorter than     951 bp
  25% of sequence in fragments shorter than    1125 bp
  30% of sequence in fragments shorter than    1300 bp
  35% of sequence in fragments shorter than    1475 bp
  40% of sequence in fragments shorter than    1666 bp
  45% of sequence in fragments shorter than    1872 bp
  50% of sequence in fragments shorter than    2098 bp
  55% of sequence in fragments shorter than    2348 bp
  60% of sequence in fragments shorter than    2632 bp
  65% of sequence in fragments shorter than    2955 bp
  70% of sequence in fragments shorter than    3338 bp
  75% of sequence in fragments shorter than    3807 bp
  80% of sequence in fragments shorter than    4407 bp
  85% of sequence in fragments shorter than    5233 bp
  90% of sequence in fragments shorter than    6560 bp
  95% of sequence in fragments shorter than    9439 bp

# NheI.SspI (GCTAGC+AATATT) double digest, 2664 thousand fragments (287384+2377267 sites), 1118 bp average fragment length
   5% of sequence in fragments shorter than     371 bp
  10% of sequence in fragments shorter than     578 bp
  15% of sequence in fragments shorter than     766 bp
  20% of sequence in fragments shorter than     949 bp
  25% of sequence in fragments shorter than    1124 bp
  30% of sequence in fragments shorter than    1306 bp
  35% of sequence in fragments shorter than    1480 bp
  40% of sequence in fragments shorter than    1677 bp
  45% of sequence in fragments shorter than    1873 bp
  50% of sequence in fragments shorter than    2100 bp
  55% of sequence in fragments shorter than    2359 bp
  60% of sequence in fragments shorter than    2651 bp
  65% of sequence in fragments shorter than    2986 bp
  70% of sequence in fragments shorter than    3380 bp
  75% of sequence in fragments shorter than    3861 bp
  80% of sequence in fragments shorter than    4484 bp
  85% of sequence in fragments shorter than    5349 bp
  90% of sequence in fragments shorter than    6730 bp
  95% of sequence in fragments shorter than    9827 bp

# HhaI.NdeI (GCGC+CATATG) double digest, 2659 thousand fragments (1756502+903360 sites), 1120 bp average fragment length
   5% of sequence in fragments shorter than     379 bp
  10% of sequence in fragments shorter than     647 bp
  15% of sequence in fragments shorter than     852 bp
  20% of sequence in fragments shorter than    1061 bp
  25% of sequence in fragments shorter than    1261 bp
  30% of sequence in fragments shorter than    1474 bp
  35% of sequence in fragments shorter than    1696 bp
  40% of sequence in fragments shorter than    1917 bp
  45% of sequence in fragments shorter than    2155 bp
  50% of sequence in fragments shorter than    2410 bp
  55% of sequence in fragments shorter than    2684 bp
  60% of sequence in fragments shorter than    2973 bp
  65% of sequence in fragments shorter than    3302 bp
  70% of sequence in fragments shorter than    3656 bp
  75% of sequence in fragments shorter than    4086 bp
  80% of sequence in fragments shorter than    4583 bp
  85% of sequence in fragments shorter than    5225 bp
  90% of sequence in fragments shorter than    6121 bp
  95% of sequence in fragments shorter than    7627 bp

# AvaI.PsiI (CYCGRG+TTATAA) double digest, 2653 thousand fragments (1005714+1647912 sites), 1123 bp average fragment length
   5% of sequence in fragments shorter than     387 bp
  10% of sequence in fragments shorter than     595 bp
  15% of sequence in fragments shorter than     777 bp
  20% of sequence in fragments shorter than     950 bp
  25% of sequence in fragments shorter than    1119 bp
  30% of sequence in fragments shorter than    1285 bp
  35% of sequence in fragments shorter than    1458 bp
  40% of sequence in fragments shorter than    1638 bp
  45% of sequence in fragments shorter than    1827 bp
  50% of sequence in fragments shorter than    2028 bp
  55% of sequence in fragments shorter than    2243 bp
  60% of sequence in fragments shorter than    2481 bp
  65% of sequence in fragments shorter than    2748 bp
  70% of sequence in fragments shorter than    3038 bp
  75% of sequence in fragments shorter than    3392 bp
  80% of sequence in fragments shorter than    3820 bp
  85% of sequence in fragments shorter than    4372 bp
  90% of sequence in fragments shorter than    5204 bp
  95% of sequence in fragments shorter than    6852 bp

# BstEII.HpaII (GGTNACC+CCGG) double digest, 2653 thousand fragments (335865+2317722 sites), 1123 bp average fragment length
   5% of sequence in fragments shorter than     348 bp
  10% of sequence in fragments shorter than     625 bp
  15% of sequence in fragments shorter than     881 bp
  20% of sequence in fragments shorter than    1136 bp
  25% of sequence in fragments shorter than    1398 bp
  30% of sequence in fragments shorter than    1673 bp
  35% of sequence in fragments shorter than    1961 bp
  40% of sequence in fragments shorter than    2273 bp
  45% of sequence in fragments shorter than    2616 bp
  50% of sequence in fragments shorter than    2991 bp
  55% of sequence in fragments shorter than    3406 bp
  60% of sequence in fragments shorter than    3865 bp
  65% of sequence in fragments shorter than    4386 bp
  70% of sequence in fragments shorter than    4994 bp
  75% of sequence in fragments shorter than    5707 bp
  80% of sequence in fragments shorter than    6595 bp
  85% of sequence in fragments shorter than    7774 bp
  90% of sequence in fragments shorter than    9527 bp
  95% of sequence in fragments shorter than   12977 bp

# AccI.HgiCI (GTMKAC+GGYRCC) double digest, 2651 thousand fragments (1101063+1550877 sites), 1123 bp average fragment length
   5% of sequence in fragments shorter than     388 bp
  10% of sequence in fragments shorter than     591 bp
  15% of sequence in fragments shorter than     766 bp
  20% of sequence in fragments shorter than     934 bp
  25% of sequence in fragments shorter than    1100 bp
  30% of sequence in fragments shorter than    1249 bp
  35% of sequence in fragments shorter than    1394 bp
  40% of sequence in fragments shorter than    1567 bp
  45% of sequence in fragments shorter than    1740 bp
  50% of sequence in fragments shorter than    1931 bp
  55% of sequence in fragments shorter than    2137 bp
  60% of sequence in fragments shorter than    2361 bp
  65% of sequence in fragments shorter than    2604 bp
  70% of sequence in fragments shorter than    2872 bp
  75% of sequence in fragments shorter than    3195 bp
  80% of sequence in fragments shorter than    3591 bp
  85% of sequence in fragments shorter than    4102 bp
  90% of sequence in fragments shorter than    4821 bp
  95% of sequence in fragments shorter than    6164 bp

# AccI.BsmI (GTMKAC+GAATGC) double digest, 2649 thousand fragments (1101063+1549350 sites), 1124 bp average fragment length
   5% of sequence in fragments shorter than     362 bp
  10% of sequence in fragments shorter than     615 bp
  15% of sequence in fragments shorter than     825 bp
  20% of sequence in fragments shorter than    1015 bp
  25% of sequence in fragments shorter than    1194 bp
  30% of sequence in fragments shorter than    1376 bp
  35% of sequence in fragments shorter than    1563 bp
  40% of sequence in fragments shorter than    1749 bp
  45% of sequence in fragments shorter than    1930 bp
  50% of sequence in fragments shorter than    2136 bp
  55% of sequence in fragments shorter than    2359 bp
  60% of sequence in fragments shorter than    2596 bp
  65% of sequence in fragments shorter than    2856 bp
  70% of sequence in fragments shorter than    3150 bp
  75% of sequence in fragments shorter than    3496 bp
  80% of sequence in fragments shorter than    3908 bp
  85% of sequence in fragments shorter than    4432 bp
  90% of sequence in fragments shorter than    5163 bp
  95% of sequence in fragments shorter than    6409 bp

# AvaIII.BfiI (ATGCAT+ACTGGG) double digest, 2639 thousand fragments (933357+1706594 sites), 1129 bp average fragment length
   5% of sequence in fragments shorter than     389 bp
  10% of sequence in fragments shorter than     598 bp
  15% of sequence in fragments shorter than     773 bp
  20% of sequence in fragments shorter than     941 bp
  25% of sequence in fragments shorter than    1102 bp
  30% of sequence in fragments shorter than    1267 bp
  35% of sequence in fragments shorter than    1434 bp
  40% of sequence in fragments shorter than    1606 bp
  45% of sequence in fragments shorter than    1784 bp
  50% of sequence in fragments shorter than    1974 bp
  55% of sequence in fragments shorter than    2171 bp
  60% of sequence in fragments shorter than    2386 bp
  65% of sequence in fragments shorter than    2629 bp
  70% of sequence in fragments shorter than    2879 bp
  75% of sequence in fragments shorter than    3200 bp
  80% of sequence in fragments shorter than    3582 bp
  85% of sequence in fragments shorter than    4086 bp
  90% of sequence in fragments shorter than    4796 bp
  95% of sequence in fragments shorter than    6048 bp

# HgiCI.PpuMI (GGYRCC+RGGWCCY) double digest, 2635 thousand fragments (1550877+1085138 sites), 1130 bp average fragment length
   5% of sequence in fragments shorter than     353 bp
  10% of sequence in fragments shorter than     573 bp
  15% of sequence in fragments shorter than     772 bp
  20% of sequence in fragments shorter than     961 bp
  25% of sequence in fragments shorter than    1155 bp
  30% of sequence in fragments shorter than    1357 bp
  35% of sequence in fragments shorter than    1569 bp
  40% of sequence in fragments shorter than    1783 bp
  45% of sequence in fragments shorter than    2020 bp
  50% of sequence in fragments shorter than    2278 bp
  55% of sequence in fragments shorter than    2558 bp
  60% of sequence in fragments shorter than    2860 bp
  65% of sequence in fragments shorter than    3204 bp
  70% of sequence in fragments shorter than    3608 bp
  75% of sequence in fragments shorter than    4092 bp
  80% of sequence in fragments shorter than    4694 bp
  85% of sequence in fragments shorter than    5492 bp
  90% of sequence in fragments shorter than    6683 bp
  95% of sequence in fragments shorter than    9182 bp

# HgiCI.PvuII (GGYRCC+CAGCTG) double digest, 2634 thousand fragments (1550877+1084260 sites), 1131 bp average fragment length
   5% of sequence in fragments shorter than     369 bp
  10% of sequence in fragments shorter than     583 bp
  15% of sequence in fragments shorter than     777 bp
  20% of sequence in fragments shorter than     966 bp
  25% of sequence in fragments shorter than    1151 bp
  30% of sequence in fragments shorter than    1331 bp
  35% of sequence in fragments shorter than    1532 bp
  40% of sequence in fragments shorter than    1734 bp
  45% of sequence in fragments shorter than    1960 bp
  50% of sequence in fragments shorter than    2203 bp
  55% of sequence in fragments shorter than    2468 bp
  60% of sequence in fragments shorter than    2753 bp
  65% of sequence in fragments shorter than    3066 bp
  70% of sequence in fragments shorter than    3444 bp
  75% of sequence in fragments shorter than    3895 bp
  80% of sequence in fragments shorter than    4447 bp
  85% of sequence in fragments shorter than    5198 bp
  90% of sequence in fragments shorter than    6299 bp
  95% of sequence in fragments shorter than    8488 bp

# BsmI.PpuMI (GAATGC+RGGWCCY) double digest, 2633 thousand fragments (1549350+1085138 sites), 1131 bp average fragment length
   5% of sequence in fragments shorter than     346 bp
  10% of sequence in fragments shorter than     602 bp
  15% of sequence in fragments shorter than     824 bp
  20% of sequence in fragments shorter than    1029 bp
  25% of sequence in fragments shorter than    1226 bp
  30% of sequence in fragments shorter than    1429 bp
  35% of sequence in fragments shorter than    1636 bp
  40% of sequence in fragments shorter than    1846 bp
  45% of sequence in fragments shorter than    2067 bp
  50% of sequence in fragments shorter than    2301 bp
  55% of sequence in fragments shorter than    2547 bp
  60% of sequence in fragments shorter than    2814 bp
  65% of sequence in fragments shorter than    3113 bp
  70% of sequence in fragments shorter than    3449 bp
  75% of sequence in fragments shorter than    3843 bp
  80% of sequence in fragments shorter than    4320 bp
  85% of sequence in fragments shorter than    4935 bp
  90% of sequence in fragments shorter than    5778 bp
  95% of sequence in fragments shorter than    7210 bp

# BsmI.PvuII (GAATGC+CAGCTG) double digest, 2633 thousand fragments (1549350+1084260 sites), 1131 bp average fragment length
   5% of sequence in fragments shorter than     350 bp
  10% of sequence in fragments shorter than     608 bp
  15% of sequence in fragments shorter than     827 bp
  20% of sequence in fragments shorter than    1025 bp
  25% of sequence in fragments shorter than    1217 bp
  30% of sequence in fragments shorter than    1411 bp
  35% of sequence in fragments shorter than    1607 bp
  40% of sequence in fragments shorter than    1810 bp
  45% of sequence in fragments shorter than    2022 bp
  50% of sequence in fragments shorter than    2244 bp
  55% of sequence in fragments shorter than    2479 bp
  60% of sequence in fragments shorter than    2738 bp
  65% of sequence in fragments shorter than    3017 bp
  70% of sequence in fragments shorter than    3334 bp
  75% of sequence in fragments shorter than    3703 bp
  80% of sequence in fragments shorter than    4152 bp
  85% of sequence in fragments shorter than    4719 bp
  90% of sequence in fragments shorter than    5515 bp
  95% of sequence in fragments shorter than    6856 bp

# BfiI.StuI (ACTGGG+AGGCCT) double digest, 2631 thousand fragments (1706594+925729 sites), 1132 bp average fragment length
   5% of sequence in fragments shorter than     357 bp
  10% of sequence in fragments shorter than     583 bp
  15% of sequence in fragments shorter than     762 bp
  20% of sequence in fragments shorter than     943 bp
  25% of sequence in fragments shorter than    1118 bp
  30% of sequence in fragments shorter than    1299 bp
  35% of sequence in fragments shorter than    1489 bp
  40% of sequence in fragments shorter than    1691 bp
  45% of sequence in fragments shorter than    1891 bp
  50% of sequence in fragments shorter than    2112 bp
  55% of sequence in fragments shorter than    2358 bp
  60% of sequence in fragments shorter than    2627 bp
  65% of sequence in fragments shorter than    2920 bp
  70% of sequence in fragments shorter than    3262 bp
  75% of sequence in fragments shorter than    3654 bp
  80% of sequence in fragments shorter than    4130 bp
  85% of sequence in fragments shorter than    4736 bp
  90% of sequence in fragments shorter than    5583 bp
  95% of sequence in fragments shorter than    7015 bp

# BspHI.PsiI (TCATGA+TTATAA) double digest, 2625 thousand fragments (978290+1647912 sites), 1134 bp average fragment length
   5% of sequence in fragments shorter than     385 bp
  10% of sequence in fragments shorter than     593 bp
  15% of sequence in fragments shorter than     776 bp
  20% of sequence in fragments shorter than     955 bp
  25% of sequence in fragments shorter than    1130 bp
  30% of sequence in fragments shorter than    1307 bp
  35% of sequence in fragments shorter than    1488 bp
  40% of sequence in fragments shorter than    1674 bp
  45% of sequence in fragments shorter than    1876 bp
  50% of sequence in fragments shorter than    2088 bp
  55% of sequence in fragments shorter than    2328 bp
  60% of sequence in fragments shorter than    2596 bp
  65% of sequence in fragments shorter than    2900 bp
  70% of sequence in fragments shorter than    3245 bp
  75% of sequence in fragments shorter than    3672 bp
  80% of sequence in fragments shorter than    4226 bp
  85% of sequence in fragments shorter than    4984 bp
  90% of sequence in fragments shorter than    6164 bp
  95% of sequence in fragments shorter than    8844 bp

# HhaI.HindIII (GCGC+AAGCTT) double digest, 2616 thousand fragments (1756502+860365 sites), 1138 bp average fragment length
   5% of sequence in fragments shorter than     382 bp
  10% of sequence in fragments shorter than     648 bp
  15% of sequence in fragments shorter than     866 bp
  20% of sequence in fragments shorter than    1085 bp
  25% of sequence in fragments shorter than    1307 bp
  30% of sequence in fragments shorter than    1528 bp
  35% of sequence in fragments shorter than    1758 bp
  40% of sequence in fragments shorter than    1976 bp
  45% of sequence in fragments shorter than    2229 bp
  50% of sequence in fragments shorter than    2489 bp
  55% of sequence in fragments shorter than    2775 bp
  60% of sequence in fragments shorter than    3090 bp
  65% of sequence in fragments shorter than    3442 bp
  70% of sequence in fragments shorter than    3841 bp
  75% of sequence in fragments shorter than    4302 bp
  80% of sequence in fragments shorter than    4857 bp
  85% of sequence in fragments shorter than    5569 bp
  90% of sequence in fragments shorter than    6568 bp
  95% of sequence in fragments shorter than    8246 bp

# HpaII.SexAI (CCGG+ACCWGGT) double digest, 2615 thousand fragments (2317722+297900 sites), 1139 bp average fragment length
   5% of sequence in fragments shorter than     353 bp
  10% of sequence in fragments shorter than     632 bp
  15% of sequence in fragments shorter than     892 bp
  20% of sequence in fragments shorter than    1151 bp
  25% of sequence in fragments shorter than    1417 bp
  30% of sequence in fragments shorter than    1691 bp
  35% of sequence in fragments shorter than    1985 bp
  40% of sequence in fragments shorter than    2299 bp
  45% of sequence in fragments shorter than    2649 bp
  50% of sequence in fragments shorter than    3025 bp
  55% of sequence in fragments shorter than    3442 bp
  60% of sequence in fragments shorter than    3908 bp
  65% of sequence in fragments shorter than    4438 bp
  70% of sequence in fragments shorter than    5056 bp
  75% of sequence in fragments shorter than    5786 bp
  80% of sequence in fragments shorter than    6689 bp
  85% of sequence in fragments shorter than    7889 bp
  90% of sequence in fragments shorter than    9656 bp
  95% of sequence in fragments shorter than   13103 bp

# BsrBI.SspI (CCGCTC+AATATT) double digest, 2612 thousand fragments (235523+2377267 sites), 1140 bp average fragment length
   5% of sequence in fragments shorter than     381 bp
  10% of sequence in fragments shorter than     597 bp
  15% of sequence in fragments shorter than     786 bp
  20% of sequence in fragments shorter than     971 bp
  25% of sequence in fragments shorter than    1153 bp
  30% of sequence in fragments shorter than    1339 bp
  35% of sequence in fragments shorter than    1524 bp
  40% of sequence in fragments shorter than    1725 bp
  45% of sequence in fragments shorter than    1939 bp
  50% of sequence in fragments shorter than    2170 bp
  55% of sequence in fragments shorter than    2422 bp
  60% of sequence in fragments shorter than    2704 bp
  65% of sequence in fragments shorter than    3023 bp
  70% of sequence in fragments shorter than    3385 bp
  75% of sequence in fragments shorter than    3817 bp
  80% of sequence in fragments shorter than    4346 bp
  85% of sequence in fragments shorter than    5032 bp
  90% of sequence in fragments shorter than    6062 bp
  95% of sequence in fragments shorter than    8027 bp

# BfiI.NdeI (ACTGGG+CATATG) double digest, 2609 thousand fragments (1706594+903360 sites), 1141 bp average fragment length
   5% of sequence in fragments shorter than     388 bp
  10% of sequence in fragments shorter than     599 bp
  15% of sequence in fragments shorter than     771 bp
  20% of sequence in fragments shorter than     943 bp
  25% of sequence in fragments shorter than    1102 bp
  30% of sequence in fragments shorter than    1271 bp
  35% of sequence in fragments shorter than    1444 bp
  40% of sequence in fragments shorter than    1622 bp
  45% of sequence in fragments shorter than    1795 bp
  50% of sequence in fragments shorter than    1990 bp
  55% of sequence in fragments shorter than    2186 bp
  60% of sequence in fragments shorter than    2408 bp
  65% of sequence in fragments shorter than    2657 bp
  70% of sequence in fragments shorter than    2942 bp
  75% of sequence in fragments shorter than    3269 bp
  80% of sequence in fragments shorter than    3670 bp
  85% of sequence in fragments shorter than    4177 bp
  90% of sequence in fragments shorter than    4916 bp
  95% of sequence in fragments shorter than    6320 bp

# AvaI.EsaBC3I (CYCGRG+TCGA) double digest, 2608 thousand fragments (1005714+1603346 sites), 1142 bp average fragment length
   5% of sequence in fragments shorter than     397 bp
  10% of sequence in fragments shorter than     636 bp
  15% of sequence in fragments shorter than     855 bp
  20% of sequence in fragments shorter than    1065 bp
  25% of sequence in fragments shorter than    1278 bp
  30% of sequence in fragments shorter than    1487 bp
  35% of sequence in fragments shorter than    1718 bp
  40% of sequence in fragments shorter than    1955 bp
  45% of sequence in fragments shorter than    2213 bp
  50% of sequence in fragments shorter than    2489 bp
  55% of sequence in fragments shorter than    2788 bp
  60% of sequence in fragments shorter than    3114 bp
  65% of sequence in fragments shorter than    3487 bp
  70% of sequence in fragments shorter than    3912 bp
  75% of sequence in fragments shorter than    4416 bp
  80% of sequence in fragments shorter than    5024 bp
  85% of sequence in fragments shorter than    5814 bp
  90% of sequence in fragments shorter than    6946 bp
  95% of sequence in fragments shorter than    8871 bp

# KasI.SspI (GGCGCC+AATATT) double digest, 2607 thousand fragments (230381+2377267 sites), 1142 bp average fragment length
   5% of sequence in fragments shorter than     377 bp
  10% of sequence in fragments shorter than     591 bp
  15% of sequence in fragments shorter than     781 bp
  20% of sequence in fragments shorter than     966 bp
  25% of sequence in fragments shorter than    1148 bp
  30% of sequence in fragments shorter than    1333 bp
  35% of sequence in fragments shorter than    1519 bp
  40% of sequence in fragments shorter than    1724 bp
  45% of sequence in fragments shorter than    1942 bp
  50% of sequence in fragments shorter than    2177 bp
  55% of sequence in fragments shorter than    2433 bp
  60% of sequence in fragments shorter than    2724 bp
  65% of sequence in fragments shorter than    3053 bp
  70% of sequence in fragments shorter than    3438 bp
  75% of sequence in fragments shorter than    3899 bp
  80% of sequence in fragments shorter than    4453 bp
  85% of sequence in fragments shorter than    5185 bp
  90% of sequence in fragments shorter than    6307 bp
  95% of sequence in fragments shorter than    8597 bp

# HhaI.XbaI (GCGC+TCTAGA) double digest, 2607 thousand fragments (1756502+850998 sites), 1143 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     655 bp
  15% of sequence in fragments shorter than     874 bp
  20% of sequence in fragments shorter than    1099 bp
  25% of sequence in fragments shorter than    1328 bp
  30% of sequence in fragments shorter than    1537 bp
  35% of sequence in fragments shorter than    1768 bp
  40% of sequence in fragments shorter than    2006 bp
  45% of sequence in fragments shorter than    2250 bp
  50% of sequence in fragments shorter than    2514 bp
  55% of sequence in fragments shorter than    2802 bp
  60% of sequence in fragments shorter than    3107 bp
  65% of sequence in fragments shorter than    3456 bp
  70% of sequence in fragments shorter than    3856 bp
  75% of sequence in fragments shorter than    4320 bp
  80% of sequence in fragments shorter than    4879 bp
  85% of sequence in fragments shorter than    5591 bp
  90% of sequence in fragments shorter than    6575 bp
  95% of sequence in fragments shorter than    8244 bp

# HpaII.KpnI (CCGG+GGTACC) double digest, 2605 thousand fragments (2317722+287974 sites), 1143 bp average fragment length
   5% of sequence in fragments shorter than     362 bp
  10% of sequence in fragments shorter than     646 bp
  15% of sequence in fragments shorter than     910 bp
  20% of sequence in fragments shorter than    1169 bp
  25% of sequence in fragments shorter than    1421 bp
  30% of sequence in fragments shorter than    1684 bp
  35% of sequence in fragments shorter than    1976 bp
  40% of sequence in fragments shorter than    2286 bp
  45% of sequence in fragments shorter than    2629 bp
  50% of sequence in fragments shorter than    2988 bp
  55% of sequence in fragments shorter than    3396 bp
  60% of sequence in fragments shorter than    3848 bp
  65% of sequence in fragments shorter than    4366 bp
  70% of sequence in fragments shorter than    4968 bp
  75% of sequence in fragments shorter than    5685 bp
  80% of sequence in fragments shorter than    6565 bp
  85% of sequence in fragments shorter than    7740 bp
  90% of sequence in fragments shorter than    9463 bp
  95% of sequence in fragments shorter than   12879 bp

# HpaII.NheI (CCGG+GCTAGC) double digest, 2604 thousand fragments (2317722+287384 sites), 1144 bp average fragment length
   5% of sequence in fragments shorter than     360 bp
  10% of sequence in fragments shorter than     643 bp
  15% of sequence in fragments shorter than     907 bp
  20% of sequence in fragments shorter than    1156 bp
  25% of sequence in fragments shorter than    1415 bp
  30% of sequence in fragments shorter than    1684 bp
  35% of sequence in fragments shorter than    1951 bp
  40% of sequence in fragments shorter than    2260 bp
  45% of sequence in fragments shorter than    2590 bp
  50% of sequence in fragments shorter than    2947 bp
  55% of sequence in fragments shorter than    3344 bp
  60% of sequence in fragments shorter than    3791 bp
  65% of sequence in fragments shorter than    4299 bp
  70% of sequence in fragments shorter than    4891 bp
  75% of sequence in fragments shorter than    5593 bp
  80% of sequence in fragments shorter than    6460 bp
  85% of sequence in fragments shorter than    7608 bp
  90% of sequence in fragments shorter than    9284 bp
  95% of sequence in fragments shorter than   12535 bp

# SspI.SwaI (AATATT+ATTTAAAT) double digest, 2603 thousand fragments (2377267+226602 sites), 1144 bp average fragment length
   5% of sequence in fragments shorter than     373 bp
  10% of sequence in fragments shorter than     592 bp
  15% of sequence in fragments shorter than     787 bp
  20% of sequence in fragments shorter than     980 bp
  25% of sequence in fragments shorter than    1172 bp
  30% of sequence in fragments shorter than    1373 bp
  35% of sequence in fragments shorter than    1574 bp
  40% of sequence in fragments shorter than    1798 bp
  45% of sequence in fragments shorter than    2038 bp
  50% of sequence in fragments shorter than    2304 bp
  55% of sequence in fragments shorter than    2598 bp
  60% of sequence in fragments shorter than    2937 bp
  65% of sequence in fragments shorter than    3331 bp
  70% of sequence in fragments shorter than    3804 bp
  75% of sequence in fragments shorter than    4369 bp
  80% of sequence in fragments shorter than    5101 bp
  85% of sequence in fragments shorter than    6193 bp
  90% of sequence in fragments shorter than    8066 bp
  95% of sequence in fragments shorter than   12921 bp

# EcoRI.HhaI (GAATTC+GCGC) double digest, 2603 thousand fragments (847401+1756502 sites), 1144 bp average fragment length
   5% of sequence in fragments shorter than     368 bp
  10% of sequence in fragments shorter than     646 bp
  15% of sequence in fragments shorter than     877 bp
  20% of sequence in fragments shorter than    1111 bp
  25% of sequence in fragments shorter than    1343 bp
  30% of sequence in fragments shorter than    1575 bp
  35% of sequence in fragments shorter than    1807 bp
  40% of sequence in fragments shorter than    2057 bp
  45% of sequence in fragments shorter than    2320 bp
  50% of sequence in fragments shorter than    2586 bp
  55% of sequence in fragments shorter than    2884 bp
  60% of sequence in fragments shorter than    3205 bp
  65% of sequence in fragments shorter than    3561 bp
  70% of sequence in fragments shorter than    3977 bp
  75% of sequence in fragments shorter than    4457 bp
  80% of sequence in fragments shorter than    5044 bp
  85% of sequence in fragments shorter than    5783 bp
  90% of sequence in fragments shorter than    6803 bp
  95% of sequence in fragments shorter than    8546 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# AccI.AflIII (GTMKAC+ACRYGT) double digest, 2585 thousand fragments (1101063+1485394 sites), 1152 bp average fragment length
   5% of sequence in fragments shorter than     412 bp
  10% of sequence in fragments shorter than     644 bp
  15% of sequence in fragments shorter than     841 bp
  20% of sequence in fragments shorter than    1028 bp
  25% of sequence in fragments shorter than    1208 bp
  30% of sequence in fragments shorter than    1386 bp
  35% of sequence in fragments shorter than    1569 bp
  40% of sequence in fragments shorter than    1758 bp
  45% of sequence in fragments shorter than    1953 bp
  50% of sequence in fragments shorter than    2160 bp
  55% of sequence in fragments shorter than    2385 bp
  60% of sequence in fragments shorter than    2633 bp
  65% of sequence in fragments shorter than    2903 bp
  70% of sequence in fragments shorter than    3214 bp
  75% of sequence in fragments shorter than    3572 bp
  80% of sequence in fragments shorter than    4013 bp
  85% of sequence in fragments shorter than    4576 bp
  90% of sequence in fragments shorter than    5368 bp
  95% of sequence in fragments shorter than    6670 bp

# BspHI.EsaBC3I (TCATGA+TCGA) double digest, 2581 thousand fragments (978290+1603346 sites), 1154 bp average fragment length
   5% of sequence in fragments shorter than     412 bp
  10% of sequence in fragments shorter than     617 bp
  15% of sequence in fragments shorter than     794 bp
  20% of sequence in fragments shorter than     965 bp
  25% of sequence in fragments shorter than    1129 bp
  30% of sequence in fragments shorter than    1293 bp
  35% of sequence in fragments shorter than    1455 bp
  40% of sequence in fragments shorter than    1630 bp
  45% of sequence in fragments shorter than    1811 bp
  50% of sequence in fragments shorter than    2000 bp
  55% of sequence in fragments shorter than    2203 bp
  60% of sequence in fragments shorter than    2422 bp
  65% of sequence in fragments shorter than    2669 bp
  70% of sequence in fragments shorter than    2942 bp
  75% of sequence in fragments shorter than    3258 bp
  80% of sequence in fragments shorter than    3644 bp
  85% of sequence in fragments shorter than    4132 bp
  90% of sequence in fragments shorter than    4817 bp
  95% of sequence in fragments shorter than    6009 bp

# AvaIII.PsiI (ATGCAT+TTATAA) double digest, 2580 thousand fragments (933357+1647912 sites), 1154 bp average fragment length
   5% of sequence in fragments shorter than     387 bp
  10% of sequence in fragments shorter than     603 bp
  15% of sequence in fragments shorter than     795 bp
  20% of sequence in fragments shorter than     979 bp
  25% of sequence in fragments shorter than    1162 bp
  30% of sequence in fragments shorter than    1351 bp
  35% of sequence in fragments shorter than    1544 bp
  40% of sequence in fragments shorter than    1750 bp
  45% of sequence in fragments shorter than    1969 bp
  50% of sequence in fragments shorter than    2204 bp
  55% of sequence in fragments shorter than    2464 bp
  60% of sequence in fragments shorter than    2755 bp
  65% of sequence in fragments shorter than    3070 bp
  70% of sequence in fragments shorter than    3450 bp
  75% of sequence in fragments shorter than    3908 bp
  80% of sequence in fragments shorter than    4499 bp
  85% of sequence in fragments shorter than    5339 bp
  90% of sequence in fragments shorter than    6648 bp
  95% of sequence in fragments shorter than    9546 bp

# PsiI.StuI (TTATAA+AGGCCT) double digest, 2573 thousand fragments (1647912+925729 sites), 1158 bp average fragment length
   5% of sequence in fragments shorter than     387 bp
  10% of sequence in fragments shorter than     616 bp
  15% of sequence in fragments shorter than     791 bp
  20% of sequence in fragments shorter than     965 bp
  25% of sequence in fragments shorter than    1144 bp
  30% of sequence in fragments shorter than    1320 bp
  35% of sequence in fragments shorter than    1488 bp
  40% of sequence in fragments shorter than    1678 bp
  45% of sequence in fragments shorter than    1868 bp
  50% of sequence in fragments shorter than    2073 bp
  55% of sequence in fragments shorter than    2298 bp
  60% of sequence in fragments shorter than    2542 bp
  65% of sequence in fragments shorter than    2813 bp
  70% of sequence in fragments shorter than    3118 bp
  75% of sequence in fragments shorter than    3471 bp
  80% of sequence in fragments shorter than    3894 bp
  85% of sequence in fragments shorter than    4430 bp
  90% of sequence in fragments shorter than    5184 bp
  95% of sequence in fragments shorter than    6488 bp

# AflIII.PpuMI (ACRYGT+RGGWCCY) double digest, 2570 thousand fragments (1485394+1085138 sites), 1159 bp average fragment length
   5% of sequence in fragments shorter than     397 bp
  10% of sequence in fragments shorter than     624 bp
  15% of sequence in fragments shorter than     822 bp
  20% of sequence in fragments shorter than    1007 bp
  25% of sequence in fragments shorter than    1191 bp
  30% of sequence in fragments shorter than    1362 bp
  35% of sequence in fragments shorter than    1548 bp
  40% of sequence in fragments shorter than    1738 bp
  45% of sequence in fragments shorter than    1938 bp
  50% of sequence in fragments shorter than    2148 bp
  55% of sequence in fragments shorter than    2380 bp
  60% of sequence in fragments shorter than    2635 bp
  65% of sequence in fragments shorter than    2913 bp
  70% of sequence in fragments shorter than    3220 bp
  75% of sequence in fragments shorter than    3572 bp
  80% of sequence in fragments shorter than    3999 bp
  85% of sequence in fragments shorter than    4571 bp
  90% of sequence in fragments shorter than    5384 bp
  95% of sequence in fragments shorter than    6922 bp

# AflIII.PvuII (ACRYGT+CAGCTG) double digest, 2569 thousand fragments (1485394+1084260 sites), 1159 bp average fragment length
   5% of sequence in fragments shorter than     401 bp
  10% of sequence in fragments shorter than     625 bp
  15% of sequence in fragments shorter than     817 bp
  20% of sequence in fragments shorter than     996 bp
  25% of sequence in fragments shorter than    1171 bp
  30% of sequence in fragments shorter than    1348 bp
  35% of sequence in fragments shorter than    1525 bp
  40% of sequence in fragments shorter than    1711 bp
  45% of sequence in fragments shorter than    1903 bp
  50% of sequence in fragments shorter than    2106 bp
  55% of sequence in fragments shorter than    2323 bp
  60% of sequence in fragments shorter than    2562 bp
  65% of sequence in fragments shorter than    2823 bp
  70% of sequence in fragments shorter than    3123 bp
  75% of sequence in fragments shorter than    3477 bp
  80% of sequence in fragments shorter than    3908 bp
  85% of sequence in fragments shorter than    4447 bp
  90% of sequence in fragments shorter than    5181 bp
  95% of sequence in fragments shorter than    6573 bp

# BfiI.HindIII (ACTGGG+AAGCTT) double digest, 2566 thousand fragments (1706594+860365 sites), 1161 bp average fragment length
   5% of sequence in fragments shorter than     397 bp
  10% of sequence in fragments shorter than     611 bp
  15% of sequence in fragments shorter than     795 bp
  20% of sequence in fragments shorter than     960 bp
  25% of sequence in fragments shorter than    1117 bp
  30% of sequence in fragments shorter than    1285 bp
  35% of sequence in fragments shorter than    1454 bp
  40% of sequence in fragments shorter than    1634 bp
  45% of sequence in fragments shorter than    1816 bp
  50% of sequence in fragments shorter than    1988 bp
  55% of sequence in fragments shorter than    2195 bp
  60% of sequence in fragments shorter than    2422 bp
  65% of sequence in fragments shorter than    2673 bp
  70% of sequence in fragments shorter than    2953 bp
  75% of sequence in fragments shorter than    3286 bp
  80% of sequence in fragments shorter than    3691 bp
  85% of sequence in fragments shorter than    4197 bp
  90% of sequence in fragments shorter than    4926 bp
  95% of sequence in fragments shorter than    6230 bp

# BfiI.XbaI (ACTGGG+TCTAGA) double digest, 2557 thousand fragments (1706594+850998 sites), 1165 bp average fragment length
   5% of sequence in fragments shorter than     403 bp
  10% of sequence in fragments shorter than     619 bp
  15% of sequence in fragments shorter than     804 bp
  20% of sequence in fragments shorter than     970 bp
  25% of sequence in fragments shorter than    1138 bp
  30% of sequence in fragments shorter than    1312 bp
  35% of sequence in fragments shorter than    1472 bp
  40% of sequence in fragments shorter than    1653 bp
  45% of sequence in fragments shorter than    1837 bp
  50% of sequence in fragments shorter than    2032 bp
  55% of sequence in fragments shorter than    2227 bp
  60% of sequence in fragments shorter than    2444 bp
  65% of sequence in fragments shorter than    2695 bp
  70% of sequence in fragments shorter than    2981 bp
  75% of sequence in fragments shorter than    3316 bp
  80% of sequence in fragments shorter than    3714 bp
  85% of sequence in fragments shorter than    4221 bp
  90% of sequence in fragments shorter than    4927 bp
  95% of sequence in fragments shorter than    6134 bp

# AvaI.HgiCI (CYCGRG+GGYRCC) double digest, 2556 thousand fragments (1005714+1550877 sites), 1165 bp average fragment length
   5% of sequence in fragments shorter than     388 bp
  10% of sequence in fragments shorter than     634 bp
  15% of sequence in fragments shorter than     859 bp
  20% of sequence in fragments shorter than    1082 bp
  25% of sequence in fragments shorter than    1293 bp
  30% of sequence in fragments shorter than    1531 bp
  35% of sequence in fragments shorter than    1768 bp
  40% of sequence in fragments shorter than    2027 bp
  45% of sequence in fragments shorter than    2310 bp
  50% of sequence in fragments shorter than    2619 bp
  55% of sequence in fragments shorter than    2947 bp
  60% of sequence in fragments shorter than    3319 bp
  65% of sequence in fragments shorter than    3752 bp
  70% of sequence in fragments shorter than    4246 bp
  75% of sequence in fragments shorter than    4836 bp
  80% of sequence in fragments shorter than    5574 bp
  85% of sequence in fragments shorter than    6563 bp
  90% of sequence in fragments shorter than    8041 bp
  95% of sequence in fragments shorter than   11075 bp

# AvaI.BsmI (CYCGRG+GAATGC) double digest, 2554 thousand fragments (1005714+1549350 sites), 1166 bp average fragment length
   5% of sequence in fragments shorter than     364 bp
  10% of sequence in fragments shorter than     634 bp
  15% of sequence in fragments shorter than     863 bp
  20% of sequence in fragments shorter than    1074 bp
  25% of sequence in fragments shorter than    1279 bp
  30% of sequence in fragments shorter than    1487 bp
  35% of sequence in fragments shorter than    1704 bp
  40% of sequence in fragments shorter than    1923 bp
  45% of sequence in fragments shorter than    2153 bp
  50% of sequence in fragments shorter than    2397 bp
  55% of sequence in fragments shorter than    2647 bp
  60% of sequence in fragments shorter than    2931 bp
  65% of sequence in fragments shorter than    3245 bp
  70% of sequence in fragments shorter than    3602 bp
  75% of sequence in fragments shorter than    4019 bp
  80% of sequence in fragments shorter than    4518 bp
  85% of sequence in fragments shorter than    5154 bp
  90% of sequence in fragments shorter than    6050 bp
  95% of sequence in fragments shorter than    7546 bp

# BfiI.EcoRI (ACTGGG+GAATTC) double digest, 2553 thousand fragments (1706594+847401 sites), 1167 bp average fragment length
   5% of sequence in fragments shorter than     388 bp
  10% of sequence in fragments shorter than     615 bp
  15% of sequence in fragments shorter than     789 bp
  20% of sequence in fragments shorter than     967 bp
  25% of sequence in fragments shorter than    1141 bp
  30% of sequence in fragments shorter than    1315 bp
  35% of sequence in fragments shorter than    1488 bp
  40% of sequence in fragments shorter than    1669 bp
  45% of sequence in fragments shorter than    1858 bp
  50% of sequence in fragments shorter than    2059 bp
  55% of sequence in fragments shorter than    2241 bp
  60% of sequence in fragments shorter than    2470 bp
  65% of sequence in fragments shorter than    2727 bp
  70% of sequence in fragments shorter than    3004 bp
  75% of sequence in fragments shorter than    3341 bp
  80% of sequence in fragments shorter than    3746 bp
  85% of sequence in fragments shorter than    4259 bp
  90% of sequence in fragments shorter than    4980 bp
  95% of sequence in fragments shorter than    6250 bp

# BsrBI.HpaII (CCGCTC+CCGG) double digest, 2552 thousand fragments (235523+2317722 sites), 1167 bp average fragment length
   5% of sequence in fragments shorter than     360 bp
  10% of sequence in fragments shorter than     663 bp
  15% of sequence in fragments shorter than     948 bp
  20% of sequence in fragments shorter than    1240 bp
  25% of sequence in fragments shorter than    1544 bp
  30% of sequence in fragments shorter than    1862 bp
  35% of sequence in fragments shorter than    2199 bp
  40% of sequence in fragments shorter than    2573 bp
  45% of sequence in fragments shorter than    2980 bp
  50% of sequence in fragments shorter than    3426 bp
  55% of sequence in fragments shorter than    3922 bp
  60% of sequence in fragments shorter than    4477 bp
  65% of sequence in fragments shorter than    5120 bp
  70% of sequence in fragments shorter than    5850 bp
  75% of sequence in fragments shorter than    6722 bp
  80% of sequence in fragments shorter than    7822 bp
  85% of sequence in fragments shorter than    9258 bp
  90% of sequence in fragments shorter than   11354 bp
  95% of sequence in fragments shorter than   15320 bp

# BscGI.PstI (CCCGT+CTGCAG) double digest, 2552 thousand fragments (1231070+1321469 sites), 1167 bp average fragment length
   5% of sequence in fragments shorter than     346 bp
  10% of sequence in fragments shorter than     565 bp
  15% of sequence in fragments shorter than     768 bp
  20% of sequence in fragments shorter than     977 bp
  25% of sequence in fragments shorter than    1188 bp
  30% of sequence in fragments shorter than    1409 bp
  35% of sequence in fragments shorter than    1639 bp
  40% of sequence in fragments shorter than    1883 bp
  45% of sequence in fragments shorter than    2142 bp
  50% of sequence in fragments shorter than    2426 bp
  55% of sequence in fragments shorter than    2732 bp
  60% of sequence in fragments shorter than    3076 bp
  65% of sequence in fragments shorter than    3461 bp
  70% of sequence in fragments shorter than    3899 bp
  75% of sequence in fragments shorter than    4418 bp
  80% of sequence in fragments shorter than    5044 bp
  85% of sequence in fragments shorter than    5836 bp
  90% of sequence in fragments shorter than    6952 bp
  95% of sequence in fragments shorter than    8911 bp

# NdeI.PsiI (CATATG+TTATAA) double digest, 2550 thousand fragments (903360+1647912 sites), 1168 bp average fragment length
   5% of sequence in fragments shorter than     385 bp
  10% of sequence in fragments shorter than     599 bp
  15% of sequence in fragments shorter than     787 bp
  20% of sequence in fragments shorter than     972 bp
  25% of sequence in fragments shorter than    1154 bp
  30% of sequence in fragments shorter than    1340 bp
  35% of sequence in fragments shorter than    1537 bp
  40% of sequence in fragments shorter than    1747 bp
  45% of sequence in fragments shorter than    1959 bp
  50% of sequence in fragments shorter than    2204 bp
  55% of sequence in fragments shorter than    2480 bp
  60% of sequence in fragments shorter than    2791 bp
  65% of sequence in fragments shorter than    3133 bp
  70% of sequence in fragments shorter than    3549 bp
  75% of sequence in fragments shorter than    4069 bp
  80% of sequence in fragments shorter than    4740 bp
  85% of sequence in fragments shorter than    5685 bp
  90% of sequence in fragments shorter than    7272 bp
  95% of sequence in fragments shorter than   11169 bp

# AclI.SspI (AACGTT+AATATT) double digest, 2549 thousand fragments (172706+2377267 sites), 1168 bp average fragment length
   5% of sequence in fragments shorter than     383 bp
  10% of sequence in fragments shorter than     603 bp
  15% of sequence in fragments shorter than     800 bp
  20% of sequence in fragments shorter than     992 bp
  25% of sequence in fragments shorter than    1179 bp
  30% of sequence in fragments shorter than    1375 bp
  35% of sequence in fragments shorter than    1574 bp
  40% of sequence in fragments shorter than    1792 bp
  45% of sequence in fragments shorter than    2026 bp
  50% of sequence in fragments shorter than    2278 bp
  55% of sequence in fragments shorter than    2562 bp
  60% of sequence in fragments shorter than    2882 bp
  65% of sequence in fragments shorter than    3240 bp
  70% of sequence in fragments shorter than    3681 bp
  75% of sequence in fragments shorter than    4214 bp
  80% of sequence in fragments shorter than    4892 bp
  85% of sequence in fragments shorter than    5868 bp
  90% of sequence in fragments shorter than    7464 bp
  95% of sequence in fragments shorter than   11221 bp

# HpaII.KasI (CCGG+GGCGCC) double digest, 2547 thousand fragments (2317722+230381 sites), 1169 bp average fragment length
   5% of sequence in fragments shorter than     367 bp
  10% of sequence in fragments shorter than     681 bp
  15% of sequence in fragments shorter than     981 bp
  20% of sequence in fragments shorter than    1284 bp
  25% of sequence in fragments shorter than    1598 bp
  30% of sequence in fragments shorter than    1930 bp
  35% of sequence in fragments shorter than    2287 bp
  40% of sequence in fragments shorter than    2684 bp
  45% of sequence in fragments shorter than    3111 bp
  50% of sequence in fragments shorter than    3580 bp
  55% of sequence in fragments shorter than    4103 bp
  60% of sequence in fragments shorter than    4692 bp
  65% of sequence in fragments shorter than    5364 bp
  70% of sequence in fragments shorter than    6136 bp
  75% of sequence in fragments shorter than    7066 bp
  80% of sequence in fragments shorter than    8231 bp
  85% of sequence in fragments shorter than    9777 bp
  90% of sequence in fragments shorter than   12077 bp
  95% of sequence in fragments shorter than   16576 bp

# HpaII.SwaI (CCGG+ATTTAAAT) double digest, 2543 thousand fragments (2317722+226602 sites), 1171 bp average fragment length
   5% of sequence in fragments shorter than     372 bp
  10% of sequence in fragments shorter than     660 bp
  15% of sequence in fragments shorter than     926 bp
  20% of sequence in fragments shorter than    1189 bp
  25% of sequence in fragments shorter than    1455 bp
  30% of sequence in fragments shorter than    1727 bp
  35% of sequence in fragments shorter than    2020 bp
  40% of sequence in fragments shorter than    2327 bp
  45% of sequence in fragments shorter than    2664 bp
  50% of sequence in fragments shorter than    3026 bp
  55% of sequence in fragments shorter than    3421 bp
  60% of sequence in fragments shorter than    3861 bp
  65% of sequence in fragments shorter than    4356 bp
  70% of sequence in fragments shorter than    4934 bp
  75% of sequence in fragments shorter than    5608 bp
  80% of sequence in fragments shorter than    6435 bp
  85% of sequence in fragments shorter than    7547 bp
  90% of sequence in fragments shorter than    9163 bp
  95% of sequence in fragments shorter than   12332 bp

# AccI.FauI (GTMKAC+CCCGC) double digest, 2540 thousand fragments (1101063+1439772 sites), 1173 bp average fragment length
   5% of sequence in fragments shorter than     410 bp
  10% of sequence in fragments shorter than     667 bp
  15% of sequence in fragments shorter than     891 bp
  20% of sequence in fragments shorter than    1105 bp
  25% of sequence in fragments shorter than    1314 bp
  30% of sequence in fragments shorter than    1508 bp
  35% of sequence in fragments shorter than    1724 bp
  40% of sequence in fragments shorter than    1946 bp
  45% of sequence in fragments shorter than    2177 bp
  50% of sequence in fragments shorter than    2426 bp
  55% of sequence in fragments shorter than    2691 bp
  60% of sequence in fragments shorter than    2983 bp
  65% of sequence in fragments shorter than    3306 bp
  70% of sequence in fragments shorter than    3677 bp
  75% of sequence in fragments shorter than    4110 bp
  80% of sequence in fragments shorter than    4600 bp
  85% of sequence in fragments shorter than    5260 bp
  90% of sequence in fragments shorter than    6139 bp
  95% of sequence in fragments shorter than    7764 bp

# AccI.BspMI (GTMKAC+ACCTGC) double digest, 2538 thousand fragments (1101063+1438129 sites), 1173 bp average fragment length
   5% of sequence in fragments shorter than     409 bp
  10% of sequence in fragments shorter than     628 bp
  15% of sequence in fragments shorter than     816 bp
  20% of sequence in fragments shorter than     991 bp
  25% of sequence in fragments shorter than    1168 bp
  30% of sequence in fragments shorter than    1343 bp
  35% of sequence in fragments shorter than    1519 bp
  40% of sequence in fragments shorter than    1702 bp
  45% of sequence in fragments shorter than    1893 bp
  50% of sequence in fragments shorter than    2079 bp
  55% of sequence in fragments shorter than    2294 bp
  60% of sequence in fragments shorter than    2526 bp
  65% of sequence in fragments shorter than    2783 bp
  70% of sequence in fragments shorter than    3076 bp
  75% of sequence in fragments shorter than    3372 bp
  80% of sequence in fragments shorter than    3772 bp
  85% of sequence in fragments shorter than    4280 bp
  90% of sequence in fragments shorter than    4986 bp
  95% of sequence in fragments shorter than    6163 bp

# PacI.SspI (TTAATTAA+AATATT) double digest, 2536 thousand fragments (159455+2377267 sites), 1174 bp average fragment length
   5% of sequence in fragments shorter than     383 bp
  10% of sequence in fragments shorter than     606 bp
  15% of sequence in fragments shorter than     806 bp
  20% of sequence in fragments shorter than    1002 bp
  25% of sequence in fragments shorter than    1198 bp
  30% of sequence in fragments shorter than    1396 bp
  35% of sequence in fragments shorter than    1607 bp
  40% of sequence in fragments shorter than    1834 bp
  45% of sequence in fragments shorter than    2076 bp
  50% of sequence in fragments shorter than    2346 bp
  55% of sequence in fragments shorter than    2644 bp
  60% of sequence in fragments shorter than    2985 bp
  65% of sequence in fragments shorter than    3382 bp
  70% of sequence in fragments shorter than    3859 bp
  75% of sequence in fragments shorter than    4422 bp
  80% of sequence in fragments shorter than    5153 bp
  85% of sequence in fragments shorter than    6241 bp
  90% of sequence in fragments shorter than    8106 bp
  95% of sequence in fragments shorter than   12862 bp

# AvaIII.EsaBC3I (ATGCAT+TCGA) double digest, 2536 thousand fragments (933357+1603346 sites), 1174 bp average fragment length
   5% of sequence in fragments shorter than     414 bp
  10% of sequence in fragments shorter than     622 bp
  15% of sequence in fragments shorter than     805 bp
  20% of sequence in fragments shorter than     978 bp
  25% of sequence in fragments shorter than    1147 bp
  30% of sequence in fragments shorter than    1316 bp
  35% of sequence in fragments shorter than    1482 bp
  40% of sequence in fragments shorter than    1660 bp
  45% of sequence in fragments shorter than    1846 bp
  50% of sequence in fragments shorter than    2043 bp
  55% of sequence in fragments shorter than    2255 bp
  60% of sequence in fragments shorter than    2486 bp
  65% of sequence in fragments shorter than    2741 bp
  70% of sequence in fragments shorter than    3024 bp
  75% of sequence in fragments shorter than    3355 bp
  80% of sequence in fragments shorter than    3755 bp
  85% of sequence in fragments shorter than    4263 bp
  90% of sequence in fragments shorter than    4968 bp
  95% of sequence in fragments shorter than    6204 bp

# Hpy99I.SspI (CGWCG+AATATT) double digest, 2534 thousand fragments (157917+2377267 sites), 1175 bp average fragment length
   5% of sequence in fragments shorter than     389 bp
  10% of sequence in fragments shorter than     612 bp
  15% of sequence in fragments shorter than     809 bp
  20% of sequence in fragments shorter than    1003 bp
  25% of sequence in fragments shorter than    1194 bp
  30% of sequence in fragments shorter than    1386 bp
  35% of sequence in fragments shorter than    1586 bp
  40% of sequence in fragments shorter than    1800 bp
  45% of sequence in fragments shorter than    2029 bp
  50% of sequence in fragments shorter than    2278 bp
  55% of sequence in fragments shorter than    2552 bp
  60% of sequence in fragments shorter than    2858 bp
  65% of sequence in fragments shorter than    3210 bp
  70% of sequence in fragments shorter than    3619 bp
  75% of sequence in fragments shorter than    4108 bp
  80% of sequence in fragments shorter than    4697 bp
  85% of sequence in fragments shorter than    5495 bp
  90% of sequence in fragments shorter than    6717 bp
  95% of sequence in fragments shorter than    9235 bp

# BglII.HhaI (AGATCT+GCGC) double digest, 2530 thousand fragments (774732+1756502 sites), 1177 bp average fragment length
   5% of sequence in fragments shorter than     396 bp
  10% of sequence in fragments shorter than     680 bp
  15% of sequence in fragments shorter than     900 bp
  20% of sequence in fragments shorter than    1129 bp
  25% of sequence in fragments shorter than    1358 bp
  30% of sequence in fragments shorter than    1586 bp
  35% of sequence in fragments shorter than    1815 bp
  40% of sequence in fragments shorter than    2062 bp
  45% of sequence in fragments shorter than    2326 bp
  50% of sequence in fragments shorter than    2607 bp
  55% of sequence in fragments shorter than    2913 bp
  60% of sequence in fragments shorter than    3247 bp
  65% of sequence in fragments shorter than    3615 bp
  70% of sequence in fragments shorter than    4021 bp
  75% of sequence in fragments shorter than    4509 bp
  80% of sequence in fragments shorter than    5096 bp
  85% of sequence in fragments shorter than    5847 bp
  90% of sequence in fragments shorter than    6898 bp
  95% of sequence in fragments shorter than    8703 bp

# BspHI.HgiCI (TCATGA+GGYRCC) double digest, 2528 thousand fragments (978290+1550877 sites), 1178 bp average fragment length
   5% of sequence in fragments shorter than     402 bp
  10% of sequence in fragments shorter than     616 bp
  15% of sequence in fragments shorter than     802 bp
  20% of sequence in fragments shorter than     977 bp
  25% of sequence in fragments shorter than    1155 bp
  30% of sequence in fragments shorter than    1326 bp
  35% of sequence in fragments shorter than    1507 bp
  40% of sequence in fragments shorter than    1685 bp
  45% of sequence in fragments shorter than    1881 bp
  50% of sequence in fragments shorter than    2086 bp
  55% of sequence in fragments shorter than    2306 bp
  60% of sequence in fragments shorter than    2550 bp
  65% of sequence in fragments shorter than    2819 bp
  70% of sequence in fragments shorter than    3121 bp
  75% of sequence in fragments shorter than    3485 bp
  80% of sequence in fragments shorter than    3923 bp
  85% of sequence in fragments shorter than    4496 bp
  90% of sequence in fragments shorter than    5344 bp
  95% of sequence in fragments shorter than    7001 bp

# EsaBC3I.StuI (TCGA+AGGCCT) double digest, 2528 thousand fragments (1603346+925729 sites), 1178 bp average fragment length
   5% of sequence in fragments shorter than     378 bp
  10% of sequence in fragments shorter than     605 bp
  15% of sequence in fragments shorter than     790 bp
  20% of sequence in fragments shorter than     976 bp
  25% of sequence in fragments shorter than    1164 bp
  30% of sequence in fragments shorter than    1352 bp
  35% of sequence in fragments shorter than    1542 bp
  40% of sequence in fragments shorter than    1743 bp
  45% of sequence in fragments shorter than    1952 bp
  50% of sequence in fragments shorter than    2180 bp
  55% of sequence in fragments shorter than    2425 bp
  60% of sequence in fragments shorter than    2692 bp
  65% of sequence in fragments shorter than    2989 bp
  70% of sequence in fragments shorter than    3327 bp
  75% of sequence in fragments shorter than    3721 bp
  80% of sequence in fragments shorter than    4192 bp
  85% of sequence in fragments shorter than    4797 bp
  90% of sequence in fragments shorter than    5638 bp
  95% of sequence in fragments shorter than    7044 bp

# BsmI.BspHI (GAATGC+TCATGA) double digest, 2527 thousand fragments (1549350+978290 sites), 1179 bp average fragment length
   5% of sequence in fragments shorter than     381 bp
  10% of sequence in fragments shorter than     651 bp
  15% of sequence in fragments shorter than     869 bp
  20% of sequence in fragments shorter than    1069 bp
  25% of sequence in fragments shorter than    1266 bp
  30% of sequence in fragments shorter than    1462 bp
  35% of sequence in fragments shorter than    1663 bp
  40% of sequence in fragments shorter than    1865 bp
  45% of sequence in fragments shorter than    2073 bp
  50% of sequence in fragments shorter than    2296 bp
  55% of sequence in fragments shorter than    2528 bp
  60% of sequence in fragments shorter than    2779 bp
  65% of sequence in fragments shorter than    3060 bp
  70% of sequence in fragments shorter than    3378 bp
  75% of sequence in fragments shorter than    3745 bp
  80% of sequence in fragments shorter than    4186 bp
  85% of sequence in fragments shorter than    4749 bp
  90% of sequence in fragments shorter than    5533 bp
  95% of sequence in fragments shorter than    6875 bp

# FauI.PpuMI (CCCGC+RGGWCCY) double digest, 2524 thousand fragments (1439772+1085138 sites), 1180 bp average fragment length
   5% of sequence in fragments shorter than     377 bp
  10% of sequence in fragments shorter than     659 bp
  15% of sequence in fragments shorter than     923 bp
  20% of sequence in fragments shorter than    1185 bp
  25% of sequence in fragments shorter than    1445 bp
  30% of sequence in fragments shorter than    1721 bp
  35% of sequence in fragments shorter than    2003 bp
  40% of sequence in fragments shorter than    2305 bp
  45% of sequence in fragments shorter than    2631 bp
  50% of sequence in fragments shorter than    2987 bp
  55% of sequence in fragments shorter than    3365 bp
  60% of sequence in fragments shorter than    3760 bp
  65% of sequence in fragments shorter than    4231 bp
  70% of sequence in fragments shorter than    4791 bp
  75% of sequence in fragments shorter than    5456 bp
  80% of sequence in fragments shorter than    6278 bp
  85% of sequence in fragments shorter than    7363 bp
  90% of sequence in fragments shorter than    8931 bp
  95% of sequence in fragments shorter than   11995 bp

# FauI.PvuII (CCCGC+CAGCTG) double digest, 2523 thousand fragments (1439772+1084260 sites), 1180 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     657 bp
  15% of sequence in fragments shorter than     903 bp
  20% of sequence in fragments shorter than    1148 bp
  25% of sequence in fragments shorter than    1390 bp
  30% of sequence in fragments shorter than    1650 bp
  35% of sequence in fragments shorter than    1919 bp
  40% of sequence in fragments shorter than    2203 bp
  45% of sequence in fragments shorter than    2509 bp
  50% of sequence in fragments shorter than    2836 bp
  55% of sequence in fragments shorter than    3196 bp
  60% of sequence in fragments shorter than    3601 bp
  65% of sequence in fragments shorter than    4046 bp
  70% of sequence in fragments shorter than    4580 bp
  75% of sequence in fragments shorter than    5220 bp
  80% of sequence in fragments shorter than    6007 bp
  85% of sequence in fragments shorter than    7026 bp
  90% of sequence in fragments shorter than    8487 bp
  95% of sequence in fragments shorter than   11285 bp

# BspMI.PpuMI (ACCTGC+RGGWCCY) double digest, 2522 thousand fragments (1438129+1085138 sites), 1181 bp average fragment length
   5% of sequence in fragments shorter than     385 bp
  10% of sequence in fragments shorter than     623 bp
  15% of sequence in fragments shorter than     833 bp
  20% of sequence in fragments shorter than    1031 bp
  25% of sequence in fragments shorter than    1236 bp
  30% of sequence in fragments shorter than    1440 bp
  35% of sequence in fragments shorter than    1639 bp
  40% of sequence in fragments shorter than    1843 bp
  45% of sequence in fragments shorter than    2063 bp
  50% of sequence in fragments shorter than    2317 bp
  55% of sequence in fragments shorter than    2595 bp
  60% of sequence in fragments shorter than    2895 bp
  65% of sequence in fragments shorter than    3238 bp
  70% of sequence in fragments shorter than    3610 bp
  75% of sequence in fragments shorter than    4062 bp
  80% of sequence in fragments shorter than    4616 bp
  85% of sequence in fragments shorter than    5327 bp
  90% of sequence in fragments shorter than    6336 bp
  95% of sequence in fragments shorter than    8117 bp

# BspMI.PvuII (ACCTGC+CAGCTG) double digest, 2521 thousand fragments (1438129+1084260 sites), 1181 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     617 bp
  15% of sequence in fragments shorter than     816 bp
  20% of sequence in fragments shorter than    1001 bp
  25% of sequence in fragments shorter than    1193 bp
  30% of sequence in fragments shorter than    1385 bp
  35% of sequence in fragments shorter than    1591 bp
  40% of sequence in fragments shorter than    1806 bp
  45% of sequence in fragments shorter than    2014 bp
  50% of sequence in fragments shorter than    2255 bp
  55% of sequence in fragments shorter than    2519 bp
  60% of sequence in fragments shorter than    2799 bp
  65% of sequence in fragments shorter than    3119 bp
  70% of sequence in fragments shorter than    3446 bp
  75% of sequence in fragments shorter than    3869 bp
  80% of sequence in fragments shorter than    4386 bp
  85% of sequence in fragments shorter than    5051 bp
  90% of sequence in fragments shorter than    5976 bp
  95% of sequence in fragments shorter than    7605 bp

# HhaI.NcoI (GCGC+CCATGG) double digest, 2515 thousand fragments (1756502+759594 sites), 1184 bp average fragment length
   5% of sequence in fragments shorter than     381 bp
  10% of sequence in fragments shorter than     673 bp
  15% of sequence in fragments shorter than     909 bp
  20% of sequence in fragments shorter than    1153 bp
  25% of sequence in fragments shorter than    1402 bp
  30% of sequence in fragments shorter than    1662 bp
  35% of sequence in fragments shorter than    1921 bp
  40% of sequence in fragments shorter than    2203 bp
  45% of sequence in fragments shorter than    2508 bp
  50% of sequence in fragments shorter than    2831 bp
  55% of sequence in fragments shorter than    3185 bp
  60% of sequence in fragments shorter than    3573 bp
  65% of sequence in fragments shorter than    3999 bp
  70% of sequence in fragments shorter than    4499 bp
  75% of sequence in fragments shorter than    5050 bp
  80% of sequence in fragments shorter than    5721 bp
  85% of sequence in fragments shorter than    6605 bp
  90% of sequence in fragments shorter than    7880 bp
  95% of sequence in fragments shorter than   10082 bp

# SnaBI.SspI (TACGTA+AATATT) double digest, 2509 thousand fragments (132299+2377267 sites), 1187 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     610 bp
  15% of sequence in fragments shorter than     811 bp
  20% of sequence in fragments shorter than    1008 bp
  25% of sequence in fragments shorter than    1201 bp
  30% of sequence in fragments shorter than    1403 bp
  35% of sequence in fragments shorter than    1613 bp
  40% of sequence in fragments shorter than    1839 bp
  45% of sequence in fragments shorter than    2081 bp
  50% of sequence in fragments shorter than    2351 bp
  55% of sequence in fragments shorter than    2648 bp
  60% of sequence in fragments shorter than    2986 bp
  65% of sequence in fragments shorter than    3381 bp
  70% of sequence in fragments shorter than    3853 bp
  75% of sequence in fragments shorter than    4412 bp
  80% of sequence in fragments shorter than    5142 bp
  85% of sequence in fragments shorter than    6214 bp
  90% of sequence in fragments shorter than    8025 bp
  95% of sequence in fragments shorter than   12495 bp

# HindIII.PsiI (AAGCTT+TTATAA) double digest, 2507 thousand fragments (860365+1647912 sites), 1188 bp average fragment length
   5% of sequence in fragments shorter than     406 bp
  10% of sequence in fragments shorter than     621 bp
  15% of sequence in fragments shorter than     817 bp
  20% of sequence in fragments shorter than     995 bp
  25% of sequence in fragments shorter than    1169 bp
  30% of sequence in fragments shorter than    1352 bp
  35% of sequence in fragments shorter than    1543 bp
  40% of sequence in fragments shorter than    1742 bp
  45% of sequence in fragments shorter than    1933 bp
  50% of sequence in fragments shorter than    2160 bp
  55% of sequence in fragments shorter than    2410 bp
  60% of sequence in fragments shorter than    2683 bp
  65% of sequence in fragments shorter than    2995 bp
  70% of sequence in fragments shorter than    3359 bp
  75% of sequence in fragments shorter than    3801 bp
  80% of sequence in fragments shorter than    4361 bp
  85% of sequence in fragments shorter than    5146 bp
  90% of sequence in fragments shorter than    6392 bp
  95% of sequence in fragments shorter than    9183 bp

# EsaBC3I.NdeI (TCGA+CATATG) double digest, 2506 thousand fragments (1603346+903360 sites), 1189 bp average fragment length
   5% of sequence in fragments shorter than     415 bp
  10% of sequence in fragments shorter than     629 bp
  15% of sequence in fragments shorter than     809 bp
  20% of sequence in fragments shorter than     981 bp
  25% of sequence in fragments shorter than    1149 bp
  30% of sequence in fragments shorter than    1307 bp
  35% of sequence in fragments shorter than    1473 bp
  40% of sequence in fragments shorter than    1651 bp
  45% of sequence in fragments shorter than    1837 bp
  50% of sequence in fragments shorter than    2037 bp
  55% of sequence in fragments shorter than    2248 bp
  60% of sequence in fragments shorter than    2479 bp
  65% of sequence in fragments shorter than    2732 bp
  70% of sequence in fragments shorter than    3017 bp
  75% of sequence in fragments shorter than    3345 bp
  80% of sequence in fragments shorter than    3744 bp
  85% of sequence in fragments shorter than    4252 bp
  90% of sequence in fragments shorter than    4961 bp
  95% of sequence in fragments shorter than    6191 bp

# NaeI.SspI (GCCGGC+AATATT) double digest, 2502 thousand fragments (125692+2377267 sites), 1190 bp average fragment length
   5% of sequence in fragments shorter than     394 bp
  10% of sequence in fragments shorter than     620 bp
  15% of sequence in fragments shorter than     820 bp
  20% of sequence in fragments shorter than    1015 bp
  25% of sequence in fragments shorter than    1209 bp
  30% of sequence in fragments shorter than    1402 bp
  35% of sequence in fragments shorter than    1606 bp
  40% of sequence in fragments shorter than    1824 bp
  45% of sequence in fragments shorter than    2056 bp
  50% of sequence in fragments shorter than    2306 bp
  55% of sequence in fragments shorter than    2582 bp
  60% of sequence in fragments shorter than    2893 bp
  65% of sequence in fragments shorter than    3247 bp
  70% of sequence in fragments shorter than    3661 bp
  75% of sequence in fragments shorter than    4152 bp
  80% of sequence in fragments shorter than    4744 bp
  85% of sequence in fragments shorter than    5555 bp
  90% of sequence in fragments shorter than    6780 bp
  95% of sequence in fragments shorter than    9307 bp

# PsiI.XbaI (TTATAA+TCTAGA) double digest, 2498 thousand fragments (1647912+850998 sites), 1192 bp average fragment length
   5% of sequence in fragments shorter than     407 bp
  10% of sequence in fragments shorter than     626 bp
  15% of sequence in fragments shorter than     818 bp
  20% of sequence in fragments shorter than     994 bp
  25% of sequence in fragments shorter than    1174 bp
  30% of sequence in fragments shorter than    1362 bp
  35% of sequence in fragments shorter than    1538 bp
  40% of sequence in fragments shorter than    1735 bp
  45% of sequence in fragments shorter than    1946 bp
  50% of sequence in fragments shorter than    2178 bp
  55% of sequence in fragments shorter than    2419 bp
  60% of sequence in fragments shorter than    2701 bp
  65% of sequence in fragments shorter than    3014 bp
  70% of sequence in fragments shorter than    3385 bp
  75% of sequence in fragments shorter than    3829 bp
  80% of sequence in fragments shorter than    4394 bp
  85% of sequence in fragments shorter than    5170 bp
  90% of sequence in fragments shorter than    6373 bp
  95% of sequence in fragments shorter than    8861 bp

# SspI.XhoI (AATATT+CTCGAG) double digest, 2498 thousand fragments (2377267+121308 sites), 1192 bp average fragment length
   5% of sequence in fragments shorter than     391 bp
  10% of sequence in fragments shorter than     616 bp
  15% of sequence in fragments shorter than     817 bp
  20% of sequence in fragments shorter than    1011 bp
  25% of sequence in fragments shorter than    1206 bp
  30% of sequence in fragments shorter than    1400 bp
  35% of sequence in fragments shorter than    1607 bp
  40% of sequence in fragments shorter than    1826 bp
  45% of sequence in fragments shorter than    2061 bp
  50% of sequence in fragments shorter than    2319 bp
  55% of sequence in fragments shorter than    2603 bp
  60% of sequence in fragments shorter than    2925 bp
  65% of sequence in fragments shorter than    3294 bp
  70% of sequence in fragments shorter than    3725 bp
  75% of sequence in fragments shorter than    4236 bp
  80% of sequence in fragments shorter than    4870 bp
  85% of sequence in fragments shorter than    5760 bp
  90% of sequence in fragments shorter than    7139 bp
  95% of sequence in fragments shorter than   10180 bp

# EcoRI.PsiI (GAATTC+TTATAA) double digest, 2494 thousand fragments (847401+1647912 sites), 1194 bp average fragment length
   5% of sequence in fragments shorter than     391 bp
  10% of sequence in fragments shorter than     619 bp
  15% of sequence in fragments shorter than     802 bp
  20% of sequence in fragments shorter than     989 bp
  25% of sequence in fragments shorter than    1176 bp
  30% of sequence in fragments shorter than    1366 bp
  35% of sequence in fragments shorter than    1556 bp
  40% of sequence in fragments shorter than    1761 bp
  45% of sequence in fragments shorter than    1975 bp
  50% of sequence in fragments shorter than    2199 bp
  55% of sequence in fragments shorter than    2454 bp
  60% of sequence in fragments shorter than    2732 bp
  65% of sequence in fragments shorter than    3040 bp
  70% of sequence in fragments shorter than    3408 bp
  75% of sequence in fragments shorter than    3852 bp
  80% of sequence in fragments shorter than    4414 bp
  85% of sequence in fragments shorter than    5188 bp
  90% of sequence in fragments shorter than    6388 bp
  95% of sequence in fragments shorter than    8964 bp

# BclI.HhaI (TGATCA+GCGC) double digest, 2494 thousand fragments (738785+1756502 sites), 1194 bp average fragment length
   5% of sequence in fragments shorter than     406 bp
  10% of sequence in fragments shorter than     687 bp
  15% of sequence in fragments shorter than     914 bp
  20% of sequence in fragments shorter than    1146 bp
  25% of sequence in fragments shorter than    1383 bp
  30% of sequence in fragments shorter than    1621 bp
  35% of sequence in fragments shorter than    1856 bp
  40% of sequence in fragments shorter than    2113 bp
  45% of sequence in fragments shorter than    2379 bp
  50% of sequence in fragments shorter than    2662 bp
  55% of sequence in fragments shorter than    2969 bp
  60% of sequence in fragments shorter than    3306 bp
  65% of sequence in fragments shorter than    3683 bp
  70% of sequence in fragments shorter than    4112 bp
  75% of sequence in fragments shorter than    4619 bp
  80% of sequence in fragments shorter than    5230 bp
  85% of sequence in fragments shorter than    6004 bp
  90% of sequence in fragments shorter than    7079 bp
  95% of sequence in fragments shorter than    8946 bp

# AflIII.AvaI (ACRYGT+CYCGRG) double digest, 2490 thousand fragments (1485394+1005714 sites), 1196 bp average fragment length
   5% of sequence in fragments shorter than     410 bp
  10% of sequence in fragments shorter than     643 bp
  15% of sequence in fragments shorter than     848 bp
  20% of sequence in fragments shorter than    1043 bp
  25% of sequence in fragments shorter than    1232 bp
  30% of sequence in fragments shorter than    1425 bp
  35% of sequence in fragments shorter than    1624 bp
  40% of sequence in fragments shorter than    1828 bp
  45% of sequence in fragments shorter than    2040 bp
  50% of sequence in fragments shorter than    2261 bp
  55% of sequence in fragments shorter than    2507 bp
  60% of sequence in fragments shorter than    2772 bp
  65% of sequence in fragments shorter than    3066 bp
  70% of sequence in fragments shorter than    3402 bp
  75% of sequence in fragments shorter than    3797 bp
  80% of sequence in fragments shorter than    4248 bp
  85% of sequence in fragments shorter than    4846 bp
  90% of sequence in fragments shorter than    5719 bp
  95% of sequence in fragments shorter than    7380 bp

# AclI.HpaII (AACGTT+CCGG) double digest, 2489 thousand fragments (172706+2317722 sites), 1196 bp average fragment length
   5% of sequence in fragments shorter than     375 bp
  10% of sequence in fragments shorter than     672 bp
  15% of sequence in fragments shorter than     952 bp
  20% of sequence in fragments shorter than    1222 bp
  25% of sequence in fragments shorter than    1503 bp
  30% of sequence in fragments shorter than    1799 bp
  35% of sequence in fragments shorter than    2107 bp
  40% of sequence in fragments shorter than    2446 bp
  45% of sequence in fragments shorter than    2817 bp
  50% of sequence in fragments shorter than    3211 bp
  55% of sequence in fragments shorter than    3649 bp
  60% of sequence in fragments shorter than    4141 bp
  65% of sequence in fragments shorter than    4707 bp
  70% of sequence in fragments shorter than    5353 bp
  75% of sequence in fragments shorter than    6118 bp
  80% of sequence in fragments shorter than    7065 bp
  85% of sequence in fragments shorter than    8291 bp
  90% of sequence in fragments shorter than   10099 bp
  95% of sequence in fragments shorter than   13396 bp

# FnuDII.HhaI (CGCG+GCGC) double digest, 2489 thousand fragments (733940+1756502 sites), 1196 bp average fragment length
   5% of sequence in fragments shorter than     504 bp
  10% of sequence in fragments shorter than     896 bp
  15% of sequence in fragments shorter than    1298 bp
  20% of sequence in fragments shorter than    1711 bp
  25% of sequence in fragments shorter than    2128 bp
  30% of sequence in fragments shorter than    2587 bp
  35% of sequence in fragments shorter than    3073 bp
  40% of sequence in fragments shorter than    3594 bp
  45% of sequence in fragments shorter than    4158 bp
  50% of sequence in fragments shorter than    4793 bp
  55% of sequence in fragments shorter than    5485 bp
  60% of sequence in fragments shorter than    6257 bp
  65% of sequence in fragments shorter than    7140 bp
  70% of sequence in fragments shorter than    8165 bp
  75% of sequence in fragments shorter than    9367 bp
  80% of sequence in fragments shorter than   10875 bp
  85% of sequence in fragments shorter than   12837 bp
  90% of sequence in fragments shorter than   15616 bp
  95% of sequence in fragments shorter than   20478 bp

# AvaIII.HgiCI (ATGCAT+GGYRCC) double digest, 2483 thousand fragments (933357+1550877 sites), 1199 bp average fragment length
   5% of sequence in fragments shorter than     414 bp
  10% of sequence in fragments shorter than     630 bp
  15% of sequence in fragments shorter than     819 bp
  20% of sequence in fragments shorter than     999 bp
  25% of sequence in fragments shorter than    1174 bp
  30% of sequence in fragments shorter than    1334 bp
  35% of sequence in fragments shorter than    1512 bp
  40% of sequence in fragments shorter than    1689 bp
  45% of sequence in fragments shorter than    1883 bp
  50% of sequence in fragments shorter than    2086 bp
  55% of sequence in fragments shorter than    2302 bp
  60% of sequence in fragments shorter than    2545 bp
  65% of sequence in fragments shorter than    2803 bp
  70% of sequence in fragments shorter than    3097 bp
  75% of sequence in fragments shorter than    3443 bp
  80% of sequence in fragments shorter than    3872 bp
  85% of sequence in fragments shorter than    4422 bp
  90% of sequence in fragments shorter than    5225 bp
  95% of sequence in fragments shorter than    6758 bp

# AvaIII.BsmI (ATGCAT+GAATGC) double digest, 2482 thousand fragments (933357+1549350 sites), 1200 bp average fragment length
   5% of sequence in fragments shorter than     408 bp
  10% of sequence in fragments shorter than     701 bp
  15% of sequence in fragments shorter than     941 bp
  20% of sequence in fragments shorter than    1158 bp
  25% of sequence in fragments shorter than    1371 bp
  30% of sequence in fragments shorter than    1585 bp
  35% of sequence in fragments shorter than    1795 bp
  40% of sequence in fragments shorter than    2014 bp
  45% of sequence in fragments shorter than    2241 bp
  50% of sequence in fragments shorter than    2477 bp
  55% of sequence in fragments shorter than    2733 bp
  60% of sequence in fragments shorter than    3006 bp
  65% of sequence in fragments shorter than    3313 bp
  70% of sequence in fragments shorter than    3663 bp
  75% of sequence in fragments shorter than    4072 bp
  80% of sequence in fragments shorter than    4557 bp
  85% of sequence in fragments shorter than    5177 bp
  90% of sequence in fragments shorter than    6055 bp
  95% of sequence in fragments shorter than    7543 bp

# BfiI.BglII (ACTGGG+AGATCT) double digest, 2480 thousand fragments (1706594+774732 sites), 1201 bp average fragment length
   5% of sequence in fragments shorter than     407 bp
  10% of sequence in fragments shorter than     628 bp
  15% of sequence in fragments shorter than     820 bp
  20% of sequence in fragments shorter than    1003 bp
  25% of sequence in fragments shorter than    1178 bp
  30% of sequence in fragments shorter than    1358 bp
  35% of sequence in fragments shorter than    1537 bp
  40% of sequence in fragments shorter than    1720 bp
  45% of sequence in fragments shorter than    1915 bp
  50% of sequence in fragments shorter than    2122 bp
  55% of sequence in fragments shorter than    2346 bp
  60% of sequence in fragments shorter than    2592 bp
  65% of sequence in fragments shorter than    2837 bp
  70% of sequence in fragments shorter than    3144 bp
  75% of sequence in fragments shorter than    3500 bp
  80% of sequence in fragments shorter than    3940 bp
  85% of sequence in fragments shorter than    4505 bp
  90% of sequence in fragments shorter than    5320 bp
  95% of sequence in fragments shorter than    6878 bp

# HpaII.PacI (CCGG+TTAATTAA) double digest, 2476 thousand fragments (2317722+159455 sites), 1203 bp average fragment length
   5% of sequence in fragments shorter than     379 bp
  10% of sequence in fragments shorter than     681 bp
  15% of sequence in fragments shorter than     963 bp
  20% of sequence in fragments shorter than    1243 bp
  25% of sequence in fragments shorter than    1528 bp
  30% of sequence in fragments shorter than    1822 bp
  35% of sequence in fragments shorter than    2136 bp
  40% of sequence in fragments shorter than    2477 bp
  45% of sequence in fragments shorter than    2846 bp
  50% of sequence in fragments shorter than    3246 bp
  55% of sequence in fragments shorter than    3684 bp
  60% of sequence in fragments shorter than    4172 bp
  65% of sequence in fragments shorter than    4729 bp
  70% of sequence in fragments shorter than    5365 bp
  75% of sequence in fragments shorter than    6123 bp
  80% of sequence in fragments shorter than    7068 bp
  85% of sequence in fragments shorter than    8305 bp
  90% of sequence in fragments shorter than   10130 bp
  95% of sequence in fragments shorter than   13714 bp

# HgiCI.StuI (GGYRCC+AGGCCT) double digest, 2476 thousand fragments (1550877+925729 sites), 1203 bp average fragment length
   5% of sequence in fragments shorter than     368 bp
  10% of sequence in fragments shorter than     609 bp
  15% of sequence in fragments shorter than     800 bp
  20% of sequence in fragments shorter than     999 bp
  25% of sequence in fragments shorter than    1200 bp
  30% of sequence in fragments shorter than    1395 bp
  35% of sequence in fragments shorter than    1605 bp
  40% of sequence in fragments shorter than    1830 bp
  45% of sequence in fragments shorter than    2072 bp
  50% of sequence in fragments shorter than    2336 bp
  55% of sequence in fragments shorter than    2623 bp
  60% of sequence in fragments shorter than    2931 bp
  65% of sequence in fragments shorter than    3281 bp
  70% of sequence in fragments shorter than    3684 bp
  75% of sequence in fragments shorter than    4158 bp
  80% of sequence in fragments shorter than    4734 bp
  85% of sequence in fragments shorter than    5470 bp
  90% of sequence in fragments shorter than    6505 bp
  95% of sequence in fragments shorter than    8284 bp

# HpaII.Hpy99I (CCGG+CGWCG) double digest, 2475 thousand fragments (2317722+157917 sites), 1203 bp average fragment length
   5% of sequence in fragments shorter than     373 bp
  10% of sequence in fragments shorter than     686 bp
  15% of sequence in fragments shorter than     986 bp
  20% of sequence in fragments shorter than    1288 bp
  25% of sequence in fragments shorter than    1602 bp
  30% of sequence in fragments shorter than    1931 bp
  35% of sequence in fragments shorter than    2284 bp
  40% of sequence in fragments shorter than    2676 bp
  45% of sequence in fragments shorter than    3094 bp
  50% of sequence in fragments shorter than    3556 bp
  55% of sequence in fragments shorter than    4069 bp
  60% of sequence in fragments shorter than    4649 bp
  65% of sequence in fragments shorter than    5313 bp
  70% of sequence in fragments shorter than    6065 bp
  75% of sequence in fragments shorter than    6984 bp
  80% of sequence in fragments shorter than    8125 bp
  85% of sequence in fragments shorter than    9628 bp
  90% of sequence in fragments shorter than   11847 bp
  95% of sequence in fragments shorter than   16064 bp

# BsmI.StuI (GAATGC+AGGCCT) double digest, 2474 thousand fragments (1549350+925729 sites), 1204 bp average fragment length
   5% of sequence in fragments shorter than     374 bp
  10% of sequence in fragments shorter than     672 bp
  15% of sequence in fragments shorter than     913 bp
  20% of sequence in fragments shorter than    1141 bp
  25% of sequence in fragments shorter than    1362 bp
  30% of sequence in fragments shorter than    1582 bp
  35% of sequence in fragments shorter than    1806 bp
  40% of sequence in fragments shorter than    2021 bp
  45% of sequence in fragments shorter than    2255 bp
  50% of sequence in fragments shorter than    2504 bp
  55% of sequence in fragments shorter than    2771 bp
  60% of sequence in fragments shorter than    3064 bp
  65% of sequence in fragments shorter than    3384 bp
  70% of sequence in fragments shorter than    3744 bp
  75% of sequence in fragments shorter than    4162 bp
  80% of sequence in fragments shorter than    4666 bp
  85% of sequence in fragments shorter than    5297 bp
  90% of sequence in fragments shorter than    6162 bp
  95% of sequence in fragments shorter than    7617 bp

# BsaAI.HhaI (YACGTR+GCGC) double digest, 2469 thousand fragments (713801+1756502 sites), 1206 bp average fragment length
   5% of sequence in fragments shorter than     394 bp
  10% of sequence in fragments shorter than     687 bp
  15% of sequence in fragments shorter than     934 bp
  20% of sequence in fragments shorter than    1193 bp
  25% of sequence in fragments shorter than    1455 bp
  30% of sequence in fragments shorter than    1724 bp
  35% of sequence in fragments shorter than    2001 bp
  40% of sequence in fragments shorter than    2299 bp
  45% of sequence in fragments shorter than    2613 bp
  50% of sequence in fragments shorter than    2954 bp
  55% of sequence in fragments shorter than    3323 bp
  60% of sequence in fragments shorter than    3714 bp
  65% of sequence in fragments shorter than    4164 bp
  70% of sequence in fragments shorter than    4680 bp
  75% of sequence in fragments shorter than    5295 bp
  80% of sequence in fragments shorter than    6042 bp
  85% of sequence in fragments shorter than    7001 bp
  90% of sequence in fragments shorter than    8366 bp
  95% of sequence in fragments shorter than   10728 bp

# BfiI.NcoI (ACTGGG+CCATGG) double digest, 2465 thousand fragments (1706594+759594 sites), 1208 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     629 bp
  15% of sequence in fragments shorter than     831 bp
  20% of sequence in fragments shorter than    1024 bp
  25% of sequence in fragments shorter than    1212 bp
  30% of sequence in fragments shorter than    1400 bp
  35% of sequence in fragments shorter than    1597 bp
  40% of sequence in fragments shorter than    1801 bp
  45% of sequence in fragments shorter than    2018 bp
  50% of sequence in fragments shorter than    2235 bp
  55% of sequence in fragments shorter than    2485 bp
  60% of sequence in fragments shorter than    2759 bp
  65% of sequence in fragments shorter than    3031 bp
  70% of sequence in fragments shorter than    3377 bp
  75% of sequence in fragments shorter than    3788 bp
  80% of sequence in fragments shorter than    4281 bp
  85% of sequence in fragments shorter than    4911 bp
  90% of sequence in fragments shorter than    5820 bp
  95% of sequence in fragments shorter than    7575 bp

# ClaI.SspI (ATCGAT+AATATT) double digest, 2463 thousand fragments (86477+2377267 sites), 1209 bp average fragment length
   5% of sequence in fragments shorter than     395 bp
  10% of sequence in fragments shorter than     622 bp
  15% of sequence in fragments shorter than     826 bp
  20% of sequence in fragments shorter than    1025 bp
  25% of sequence in fragments shorter than    1224 bp
  30% of sequence in fragments shorter than    1425 bp
  35% of sequence in fragments shorter than    1636 bp
  40% of sequence in fragments shorter than    1865 bp
  45% of sequence in fragments shorter than    2110 bp
  50% of sequence in fragments shorter than    2381 bp
  55% of sequence in fragments shorter than    2681 bp
  60% of sequence in fragments shorter than    3021 bp
  65% of sequence in fragments shorter than    3417 bp
  70% of sequence in fragments shorter than    3889 bp
  75% of sequence in fragments shorter than    4455 bp
  80% of sequence in fragments shorter than    5190 bp
  85% of sequence in fragments shorter than    6263 bp
  90% of sequence in fragments shorter than    8058 bp
  95% of sequence in fragments shorter than   12457 bp

# AflIII.BspHI (ACRYGT+TCATGA) double digest, 2463 thousand fragments (1485394+978290 sites), 1209 bp average fragment length
   5% of sequence in fragments shorter than     427 bp
  10% of sequence in fragments shorter than     656 bp
  15% of sequence in fragments shorter than     853 bp
  20% of sequence in fragments shorter than    1038 bp
  25% of sequence in fragments shorter than    1214 bp
  30% of sequence in fragments shorter than    1392 bp
  35% of sequence in fragments shorter than    1578 bp
  40% of sequence in fragments shorter than    1768 bp
  45% of sequence in fragments shorter than    1963 bp
  50% of sequence in fragments shorter than    2176 bp
  55% of sequence in fragments shorter than    2403 bp
  60% of sequence in fragments shorter than    2654 bp
  65% of sequence in fragments shorter than    2930 bp
  70% of sequence in fragments shorter than    3240 bp
  75% of sequence in fragments shorter than    3612 bp
  80% of sequence in fragments shorter than    4070 bp
  85% of sequence in fragments shorter than    4652 bp
  90% of sequence in fragments shorter than    5468 bp
  95% of sequence in fragments shorter than    6993 bp

# EsaBC3I.HindIII (TCGA+AAGCTT) double digest, 2463 thousand fragments (1603346+860365 sites), 1209 bp average fragment length
   5% of sequence in fragments shorter than     418 bp
  10% of sequence in fragments shorter than     633 bp
  15% of sequence in fragments shorter than     825 bp
  20% of sequence in fragments shorter than    1006 bp
  25% of sequence in fragments shorter than    1179 bp
  30% of sequence in fragments shorter than    1354 bp
  35% of sequence in fragments shorter than    1528 bp
  40% of sequence in fragments shorter than    1712 bp
  45% of sequence in fragments shorter than    1902 bp
  50% of sequence in fragments shorter than    2107 bp
  55% of sequence in fragments shorter than    2331 bp
  60% of sequence in fragments shorter than    2568 bp
  65% of sequence in fragments shorter than    2830 bp
  70% of sequence in fragments shorter than    3125 bp
  75% of sequence in fragments shorter than    3466 bp
  80% of sequence in fragments shorter than    3881 bp
  85% of sequence in fragments shorter than    4412 bp
  90% of sequence in fragments shorter than    5150 bp
  95% of sequence in fragments shorter than    6421 bp

# BceAI.HhaI (ACGGC+GCGC) double digest, 2461 thousand fragments (705178+1756502 sites), 1210 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     689 bp
  15% of sequence in fragments shorter than     972 bp
  20% of sequence in fragments shorter than    1260 bp
  25% of sequence in fragments shorter than    1563 bp
  30% of sequence in fragments shorter than    1874 bp
  35% of sequence in fragments shorter than    2212 bp
  40% of sequence in fragments shorter than    2572 bp
  45% of sequence in fragments shorter than    2964 bp
  50% of sequence in fragments shorter than    3393 bp
  55% of sequence in fragments shorter than    3863 bp
  60% of sequence in fragments shorter than    4381 bp
  65% of sequence in fragments shorter than    4979 bp
  70% of sequence in fragments shorter than    5658 bp
  75% of sequence in fragments shorter than    6466 bp
  80% of sequence in fragments shorter than    7455 bp
  85% of sequence in fragments shorter than    8733 bp
  90% of sequence in fragments shorter than   10574 bp
  95% of sequence in fragments shorter than   13779 bp

# EsaBC3I.XbaI (TCGA+TCTAGA) double digest, 2453 thousand fragments (1603346+850998 sites), 1214 bp average fragment length
   5% of sequence in fragments shorter than     422 bp
  10% of sequence in fragments shorter than     644 bp
  15% of sequence in fragments shorter than     829 bp
  20% of sequence in fragments shorter than    1007 bp
  25% of sequence in fragments shorter than    1182 bp
  30% of sequence in fragments shorter than    1353 bp
  35% of sequence in fragments shorter than    1523 bp
  40% of sequence in fragments shorter than    1705 bp
  45% of sequence in fragments shorter than    1892 bp
  50% of sequence in fragments shorter than    2092 bp
  55% of sequence in fragments shorter than    2305 bp
  60% of sequence in fragments shorter than    2543 bp
  65% of sequence in fragments shorter than    2807 bp
  70% of sequence in fragments shorter than    3099 bp
  75% of sequence in fragments shorter than    3441 bp
  80% of sequence in fragments shorter than    3851 bp
  85% of sequence in fragments shorter than    4370 bp
  90% of sequence in fragments shorter than    5080 bp
  95% of sequence in fragments shorter than    6309 bp

# HgiCI.NdeI (GGYRCC+CATATG) double digest, 2453 thousand fragments (1550877+903360 sites), 1214 bp average fragment length
   5% of sequence in fragments shorter than     414 bp
  10% of sequence in fragments shorter than     638 bp
  15% of sequence in fragments shorter than     826 bp
  20% of sequence in fragments shorter than    1008 bp
  25% of sequence in fragments shorter than    1169 bp
  30% of sequence in fragments shorter than    1339 bp
  35% of sequence in fragments shorter than    1522 bp
  40% of sequence in fragments shorter than    1704 bp
  45% of sequence in fragments shorter than    1903 bp
  50% of sequence in fragments shorter than    2116 bp
  55% of sequence in fragments shorter than    2343 bp
  60% of sequence in fragments shorter than    2593 bp
  65% of sequence in fragments shorter than    2860 bp
  70% of sequence in fragments shorter than    3165 bp
  75% of sequence in fragments shorter than    3529 bp
  80% of sequence in fragments shorter than    3975 bp
  85% of sequence in fragments shorter than    4557 bp
  90% of sequence in fragments shorter than    5415 bp
  95% of sequence in fragments shorter than    7109 bp

# BsmI.NdeI (GAATGC+CATATG) double digest, 2452 thousand fragments (1549350+903360 sites), 1215 bp average fragment length
   5% of sequence in fragments shorter than     376 bp
  10% of sequence in fragments shorter than     659 bp
  15% of sequence in fragments shorter than     890 bp
  20% of sequence in fragments shorter than    1100 bp
  25% of sequence in fragments shorter than    1299 bp
  30% of sequence in fragments shorter than    1505 bp
  35% of sequence in fragments shorter than    1712 bp
  40% of sequence in fragments shorter than    1926 bp
  45% of sequence in fragments shorter than    2152 bp
  50% of sequence in fragments shorter than    2390 bp
  55% of sequence in fragments shorter than    2639 bp
  60% of sequence in fragments shorter than    2907 bp
  65% of sequence in fragments shorter than    3212 bp
  70% of sequence in fragments shorter than    3560 bp
  75% of sequence in fragments shorter than    3964 bp
  80% of sequence in fragments shorter than    4449 bp
  85% of sequence in fragments shorter than    5068 bp
  90% of sequence in fragments shorter than    5939 bp
  95% of sequence in fragments shorter than    7454 bp

# BciVI.HhaI (GTATCC+GCGC) double digest, 2452 thousand fragments (696093+1756502 sites), 1215 bp average fragment length
   5% of sequence in fragments shorter than     410 bp
  10% of sequence in fragments shorter than     690 bp
  15% of sequence in fragments shorter than     941 bp
  20% of sequence in fragments shorter than    1184 bp
  25% of sequence in fragments shorter than    1426 bp
  30% of sequence in fragments shorter than    1664 bp
  35% of sequence in fragments shorter than    1903 bp
  40% of sequence in fragments shorter than    2164 bp
  45% of sequence in fragments shorter than    2440 bp
  50% of sequence in fragments shorter than    2738 bp
  55% of sequence in fragments shorter than    3060 bp
  60% of sequence in fragments shorter than    3412 bp
  65% of sequence in fragments shorter than    3802 bp
  70% of sequence in fragments shorter than    4239 bp
  75% of sequence in fragments shorter than    4762 bp
  80% of sequence in fragments shorter than    5397 bp
  85% of sequence in fragments shorter than    6199 bp
  90% of sequence in fragments shorter than    7321 bp
  95% of sequence in fragments shorter than    9267 bp

# EcoRI.EsaBC3I (GAATTC+TCGA) double digest, 2450 thousand fragments (847401+1603346 sites), 1216 bp average fragment length
   5% of sequence in fragments shorter than     415 bp
  10% of sequence in fragments shorter than     641 bp
  15% of sequence in fragments shorter than     829 bp
  20% of sequence in fragments shorter than    1015 bp
  25% of sequence in fragments shorter than    1197 bp
  30% of sequence in fragments shorter than    1377 bp
  35% of sequence in fragments shorter than    1554 bp
  40% of sequence in fragments shorter than    1745 bp
  45% of sequence in fragments shorter than    1944 bp
  50% of sequence in fragments shorter than    2156 bp
  55% of sequence in fragments shorter than    2382 bp
  60% of sequence in fragments shorter than    2629 bp
  65% of sequence in fragments shorter than    2898 bp
  70% of sequence in fragments shorter than    3202 bp
  75% of sequence in fragments shorter than    3552 bp
  80% of sequence in fragments shorter than    3974 bp
  85% of sequence in fragments shorter than    4510 bp
  90% of sequence in fragments shorter than    5244 bp
  95% of sequence in fragments shorter than    6480 bp

# HpaII.SnaBI (CCGG+TACGTA) double digest, 2449 thousand fragments (2317722+132299 sites), 1216 bp average fragment length
   5% of sequence in fragments shorter than     381 bp
  10% of sequence in fragments shorter than     686 bp
  15% of sequence in fragments shorter than     972 bp
  20% of sequence in fragments shorter than    1258 bp
  25% of sequence in fragments shorter than    1546 bp
  30% of sequence in fragments shorter than    1848 bp
  35% of sequence in fragments shorter than    2169 bp
  40% of sequence in fragments shorter than    2521 bp
  45% of sequence in fragments shorter than    2899 bp
  50% of sequence in fragments shorter than    3312 bp
  55% of sequence in fragments shorter than    3770 bp
  60% of sequence in fragments shorter than    4277 bp
  65% of sequence in fragments shorter than    4862 bp
  70% of sequence in fragments shorter than    5533 bp
  75% of sequence in fragments shorter than    6322 bp
  80% of sequence in fragments shorter than    7317 bp
  85% of sequence in fragments shorter than    8626 bp
  90% of sequence in fragments shorter than   10534 bp
  95% of sequence in fragments shorter than   14149 bp

# AatII.SspI (GACGTC+AATATT) double digest, 2447 thousand fragments (71089+2377267 sites), 1217 bp average fragment length
   5% of sequence in fragments shorter than     400 bp
  10% of sequence in fragments shorter than     629 bp
  15% of sequence in fragments shorter than     833 bp
  20% of sequence in fragments shorter than    1033 bp
  25% of sequence in fragments shorter than    1234 bp
  30% of sequence in fragments shorter than    1434 bp
  35% of sequence in fragments shorter than    1646 bp
  40% of sequence in fragments shorter than    1875 bp
  45% of sequence in fragments shorter than    2116 bp
  50% of sequence in fragments shorter than    2385 bp
  55% of sequence in fragments shorter than    2682 bp
  60% of sequence in fragments shorter than    3015 bp
  65% of sequence in fragments shorter than    3402 bp
  70% of sequence in fragments shorter than    3860 bp
  75% of sequence in fragments shorter than    4399 bp
  80% of sequence in fragments shorter than    5081 bp
  85% of sequence in fragments shorter than    6055 bp
  90% of sequence in fragments shorter than    7618 bp
  95% of sequence in fragments shorter than   11144 bp

# AvaI.FauI (CYCGRG+CCCGC) double digest, 2445 thousand fragments (1005714+1439772 sites), 1218 bp average fragment length
   5% of sequence in fragments shorter than     396 bp
  10% of sequence in fragments shorter than     722 bp
  15% of sequence in fragments shorter than    1033 bp
  20% of sequence in fragments shorter than    1351 bp
  25% of sequence in fragments shorter than    1681 bp
  30% of sequence in fragments shorter than    2036 bp
  35% of sequence in fragments shorter than    2417 bp
  40% of sequence in fragments shorter than    2836 bp
  45% of sequence in fragments shorter than    3277 bp
  50% of sequence in fragments shorter than    3782 bp
  55% of sequence in fragments shorter than    4340 bp
  60% of sequence in fragments shorter than    4968 bp
  65% of sequence in fragments shorter than    5688 bp
  70% of sequence in fragments shorter than    6537 bp
  75% of sequence in fragments shorter than    7549 bp
  80% of sequence in fragments shorter than    8779 bp
  85% of sequence in fragments shorter than   10421 bp
  90% of sequence in fragments shorter than   12830 bp
  95% of sequence in fragments shorter than   17439 bp

# BclI.BfiI (TGATCA+ACTGGG) double digest, 2444 thousand fragments (738785+1706594 sites), 1218 bp average fragment length
   5% of sequence in fragments shorter than     417 bp
  10% of sequence in fragments shorter than     643 bp
  15% of sequence in fragments shorter than     836 bp
  20% of sequence in fragments shorter than    1006 bp
  25% of sequence in fragments shorter than    1181 bp
  30% of sequence in fragments shorter than    1360 bp
  35% of sequence in fragments shorter than    1540 bp
  40% of sequence in fragments shorter than    1722 bp
  45% of sequence in fragments shorter than    1898 bp
  50% of sequence in fragments shorter than    2098 bp
  55% of sequence in fragments shorter than    2314 bp
  60% of sequence in fragments shorter than    2555 bp
  65% of sequence in fragments shorter than    2815 bp
  70% of sequence in fragments shorter than    3116 bp
  75% of sequence in fragments shorter than    3470 bp
  80% of sequence in fragments shorter than    3902 bp
  85% of sequence in fragments shorter than    4452 bp
  90% of sequence in fragments shorter than    5248 bp
  95% of sequence in fragments shorter than    6784 bp

# SacII.SspI (CCGCGG+AATATT) double digest, 2443 thousand fragments (66901+2377267 sites), 1219 bp average fragment length
   5% of sequence in fragments shorter than     400 bp
  10% of sequence in fragments shorter than     631 bp
  15% of sequence in fragments shorter than     837 bp
  20% of sequence in fragments shorter than    1039 bp
  25% of sequence in fragments shorter than    1241 bp
  30% of sequence in fragments shorter than    1441 bp
  35% of sequence in fragments shorter than    1657 bp
  40% of sequence in fragments shorter than    1886 bp
  45% of sequence in fragments shorter than    2129 bp
  50% of sequence in fragments shorter than    2399 bp
  55% of sequence in fragments shorter than    2698 bp
  60% of sequence in fragments shorter than    3035 bp
  65% of sequence in fragments shorter than    3423 bp
  70% of sequence in fragments shorter than    3884 bp
  75% of sequence in fragments shorter than    4421 bp
  80% of sequence in fragments shorter than    5101 bp
  85% of sequence in fragments shorter than    6075 bp
  90% of sequence in fragments shorter than    7604 bp
  95% of sequence in fragments shorter than   10941 bp

# AvaI.BspMI (CYCGRG+ACCTGC) double digest, 2443 thousand fragments (1005714+1438129 sites), 1219 bp average fragment length
   5% of sequence in fragments shorter than     393 bp
  10% of sequence in fragments shorter than     636 bp
  15% of sequence in fragments shorter than     851 bp
  20% of sequence in fragments shorter than    1056 bp
  25% of sequence in fragments shorter than    1266 bp
  30% of sequence in fragments shorter than    1482 bp
  35% of sequence in fragments shorter than    1715 bp
  40% of sequence in fragments shorter than    1959 bp
  45% of sequence in fragments shorter than    2208 bp
  50% of sequence in fragments shorter than    2487 bp
  55% of sequence in fragments shorter than    2788 bp
  60% of sequence in fragments shorter than    3130 bp
  65% of sequence in fragments shorter than    3463 bp
  70% of sequence in fragments shorter than    3891 bp
  75% of sequence in fragments shorter than    4398 bp
  80% of sequence in fragments shorter than    5010 bp
  85% of sequence in fragments shorter than    5803 bp
  90% of sequence in fragments shorter than    6944 bp
  95% of sequence in fragments shorter than    8944 bp

# HpaII.NaeI (CCGG+GCCGGC) double digest, 2442 thousand fragments (2317722+125692 sites), 1219 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     727 bp
  15% of sequence in fragments shorter than    1041 bp
  20% of sequence in fragments shorter than    1359 bp
  25% of sequence in fragments shorter than    1684 bp
  30% of sequence in fragments shorter than    2035 bp
  35% of sequence in fragments shorter than    2406 bp
  40% of sequence in fragments shorter than    2817 bp
  45% of sequence in fragments shorter than    3263 bp
  50% of sequence in fragments shorter than    3751 bp
  55% of sequence in fragments shorter than    4291 bp
  60% of sequence in fragments shorter than    4906 bp
  65% of sequence in fragments shorter than    5603 bp
  70% of sequence in fragments shorter than    6398 bp
  75% of sequence in fragments shorter than    7370 bp
  80% of sequence in fragments shorter than    8590 bp
  85% of sequence in fragments shorter than   10188 bp
  90% of sequence in fragments shorter than   12542 bp
  95% of sequence in fragments shorter than   17302 bp

# BfiI.FnuDII (ACTGGG+CGCG) double digest, 2440 thousand fragments (1706594+733940 sites), 1221 bp average fragment length
   5% of sequence in fragments shorter than     413 bp
  10% of sequence in fragments shorter than     681 bp
  15% of sequence in fragments shorter than     911 bp
  20% of sequence in fragments shorter than    1126 bp
  25% of sequence in fragments shorter than    1348 bp
  30% of sequence in fragments shorter than    1571 bp
  35% of sequence in fragments shorter than    1797 bp
  40% of sequence in fragments shorter than    2035 bp
  45% of sequence in fragments shorter than    2268 bp
  50% of sequence in fragments shorter than    2537 bp
  55% of sequence in fragments shorter than    2801 bp
  60% of sequence in fragments shorter than    3123 bp
  65% of sequence in fragments shorter than    3481 bp
  70% of sequence in fragments shorter than    3894 bp
  75% of sequence in fragments shorter than    4382 bp
  80% of sequence in fragments shorter than    4967 bp
  85% of sequence in fragments shorter than    5732 bp
  90% of sequence in fragments shorter than    6860 bp
  95% of sequence in fragments shorter than    9084 bp

# HpaII.XhoI (CCGG+CTCGAG) double digest, 2438 thousand fragments (2317722+121308 sites), 1222 bp average fragment length
   5% of sequence in fragments shorter than     375 bp
  10% of sequence in fragments shorter than     684 bp
  15% of sequence in fragments shorter than     976 bp
  20% of sequence in fragments shorter than    1273 bp
  25% of sequence in fragments shorter than    1581 bp
  30% of sequence in fragments shorter than    1906 bp
  35% of sequence in fragments shorter than    2251 bp
  40% of sequence in fragments shorter than    2632 bp
  45% of sequence in fragments shorter than    3050 bp
  50% of sequence in fragments shorter than    3507 bp
  55% of sequence in fragments shorter than    4013 bp
  60% of sequence in fragments shorter than    4576 bp
  65% of sequence in fragments shorter than    5223 bp
  70% of sequence in fragments shorter than    5964 bp
  75% of sequence in fragments shorter than    6864 bp
  80% of sequence in fragments shorter than    7988 bp
  85% of sequence in fragments shorter than    9461 bp
  90% of sequence in fragments shorter than   11644 bp
  95% of sequence in fragments shorter than   15977 bp

# PspXI.SspI (VCTCGAGB+AATATT) double digest, 2436 thousand fragments (59193+2377267 sites), 1223 bp average fragment length
   5% of sequence in fragments shorter than     401 bp
  10% of sequence in fragments shorter than     632 bp
  15% of sequence in fragments shorter than     837 bp
  20% of sequence in fragments shorter than    1038 bp
  25% of sequence in fragments shorter than    1239 bp
  30% of sequence in fragments shorter than    1439 bp
  35% of sequence in fragments shorter than    1654 bp
  40% of sequence in fragments shorter than    1883 bp
  45% of sequence in fragments shorter than    2127 bp
  50% of sequence in fragments shorter than    2397 bp
  55% of sequence in fragments shorter than    2696 bp
  60% of sequence in fragments shorter than    3034 bp
  65% of sequence in fragments shorter than    3424 bp
  70% of sequence in fragments shorter than    3884 bp
  75% of sequence in fragments shorter than    4424 bp
  80% of sequence in fragments shorter than    5112 bp
  85% of sequence in fragments shorter than    6098 bp
  90% of sequence in fragments shorter than    7688 bp
  95% of sequence in fragments shorter than   11219 bp

# AgeI.SspI (ACCGGT+AATATT) double digest, 2430 thousand fragments (53386+2377267 sites), 1226 bp average fragment length
   5% of sequence in fragments shorter than     402 bp
  10% of sequence in fragments shorter than     632 bp
  15% of sequence in fragments shorter than     838 bp
  20% of sequence in fragments shorter than    1040 bp
  25% of sequence in fragments shorter than    1244 bp
  30% of sequence in fragments shorter than    1445 bp
  35% of sequence in fragments shorter than    1663 bp
  40% of sequence in fragments shorter than    1895 bp
  45% of sequence in fragments shorter than    2142 bp
  50% of sequence in fragments shorter than    2414 bp
  55% of sequence in fragments shorter than    2718 bp
  60% of sequence in fragments shorter than    3063 bp
  65% of sequence in fragments shorter than    3460 bp
  70% of sequence in fragments shorter than    3936 bp
  75% of sequence in fragments shorter than    4492 bp
  80% of sequence in fragments shorter than    5215 bp
  85% of sequence in fragments shorter than    6260 bp
  90% of sequence in fragments shorter than    7994 bp
  95% of sequence in fragments shorter than   12042 bp

# BglII.PsiI (AGATCT+TTATAA) double digest, 2422 thousand fragments (774732+1647912 sites), 1230 bp average fragment length
   5% of sequence in fragments shorter than     418 bp
  10% of sequence in fragments shorter than     641 bp
  15% of sequence in fragments shorter than     841 bp
  20% of sequence in fragments shorter than    1032 bp
  25% of sequence in fragments shorter than    1219 bp
  30% of sequence in fragments shorter than    1407 bp
  35% of sequence in fragments shorter than    1600 bp
  40% of sequence in fragments shorter than    1801 bp
  45% of sequence in fragments shorter than    2020 bp
  50% of sequence in fragments shorter than    2258 bp
  55% of sequence in fragments shorter than    2520 bp
  60% of sequence in fragments shorter than    2795 bp
  65% of sequence in fragments shorter than    3114 bp
  70% of sequence in fragments shorter than    3499 bp
  75% of sequence in fragments shorter than    3957 bp
  80% of sequence in fragments shorter than    4537 bp
  85% of sequence in fragments shorter than    5347 bp
  90% of sequence in fragments shorter than    6642 bp
  95% of sequence in fragments shorter than    9627 bp

# AccI.PstI (GTMKAC+CTGCAG) double digest, 2422 thousand fragments (1101063+1321469 sites), 1230 bp average fragment length
   5% of sequence in fragments shorter than     429 bp
  10% of sequence in fragments shorter than     652 bp
  15% of sequence in fragments shorter than     843 bp
  20% of sequence in fragments shorter than    1028 bp
  25% of sequence in fragments shorter than    1207 bp
  30% of sequence in fragments shorter than    1380 bp
  35% of sequence in fragments shorter than    1560 bp
  40% of sequence in fragments shorter than    1749 bp
  45% of sequence in fragments shorter than    1944 bp
  50% of sequence in fragments shorter than    2150 bp
  55% of sequence in fragments shorter than    2376 bp
  60% of sequence in fragments shorter than    2617 bp
  65% of sequence in fragments shorter than    2886 bp
  70% of sequence in fragments shorter than    3193 bp
  75% of sequence in fragments shorter than    3545 bp
  80% of sequence in fragments shorter than    3972 bp
  85% of sequence in fragments shorter than    4510 bp
  90% of sequence in fragments shorter than    5194 bp
  95% of sequence in fragments shorter than    6443 bp

# BfiI.BsaAI (ACTGGG+YACGTR) double digest, 2419 thousand fragments (1706594+713801 sites), 1231 bp average fragment length
   5% of sequence in fragments shorter than     419 bp
  10% of sequence in fragments shorter than     650 bp
  15% of sequence in fragments shorter than     849 bp
  20% of sequence in fragments shorter than    1034 bp
  25% of sequence in fragments shorter than    1223 bp
  30% of sequence in fragments shorter than    1408 bp
  35% of sequence in fragments shorter than    1596 bp
  40% of sequence in fragments shorter than    1798 bp
  45% of sequence in fragments shorter than    2005 bp
  50% of sequence in fragments shorter than    2225 bp
  55% of sequence in fragments shorter than    2467 bp
  60% of sequence in fragments shorter than    2734 bp
  65% of sequence in fragments shorter than    2995 bp
  70% of sequence in fragments shorter than    3326 bp
  75% of sequence in fragments shorter than    3711 bp
  80% of sequence in fragments shorter than    4181 bp
  85% of sequence in fragments shorter than    4786 bp
  90% of sequence in fragments shorter than    5635 bp
  95% of sequence in fragments shorter than    7227 bp

# PmeI.SspI (GTTTAAAC+AATATT) double digest, 2418 thousand fragments (42094+2377267 sites), 1231 bp average fragment length
   5% of sequence in fragments shorter than     401 bp
  10% of sequence in fragments shorter than     632 bp
  15% of sequence in fragments shorter than     839 bp
  20% of sequence in fragments shorter than    1042 bp
  25% of sequence in fragments shorter than    1248 bp
  30% of sequence in fragments shorter than    1453 bp
  35% of sequence in fragments shorter than    1674 bp
  40% of sequence in fragments shorter than    1908 bp
  45% of sequence in fragments shorter than    2161 bp
  50% of sequence in fragments shorter than    2438 bp
  55% of sequence in fragments shorter than    2753 bp
  60% of sequence in fragments shorter than    3109 bp
  65% of sequence in fragments shorter than    3522 bp
  70% of sequence in fragments shorter than    4018 bp
  75% of sequence in fragments shorter than    4602 bp
  80% of sequence in fragments shorter than    5377 bp
  85% of sequence in fragments shorter than    6510 bp
  90% of sequence in fragments shorter than    8474 bp
  95% of sequence in fragments shorter than   13405 bp

# AflIII.AvaIII (ACRYGT+ATGCAT) double digest, 2418 thousand fragments (1485394+933357 sites), 1232 bp average fragment length
   5% of sequence in fragments shorter than     430 bp
  10% of sequence in fragments shorter than     674 bp
  15% of sequence in fragments shorter than     883 bp
  20% of sequence in fragments shorter than    1079 bp
  25% of sequence in fragments shorter than    1269 bp
  30% of sequence in fragments shorter than    1463 bp
  35% of sequence in fragments shorter than    1662 bp
  40% of sequence in fragments shorter than    1866 bp
  45% of sequence in fragments shorter than    2078 bp
  50% of sequence in fragments shorter than    2307 bp
  55% of sequence in fragments shorter than    2550 bp
  60% of sequence in fragments shorter than    2814 bp
  65% of sequence in fragments shorter than    3107 bp
  70% of sequence in fragments shorter than    3449 bp
  75% of sequence in fragments shorter than    3852 bp
  80% of sequence in fragments shorter than    4351 bp
  85% of sequence in fragments shorter than    4998 bp
  90% of sequence in fragments shorter than    5935 bp
  95% of sequence in fragments shorter than    7565 bp

# BspHI.FauI (TCATGA+CCCGC) double digest, 2417 thousand fragments (978290+1439772 sites), 1232 bp average fragment length
   5% of sequence in fragments shorter than     437 bp
  10% of sequence in fragments shorter than     712 bp
  15% of sequence in fragments shorter than     947 bp
  20% of sequence in fragments shorter than    1177 bp
  25% of sequence in fragments shorter than    1393 bp
  30% of sequence in fragments shorter than    1622 bp
  35% of sequence in fragments shorter than    1853 bp
  40% of sequence in fragments shorter than    2087 bp
  45% of sequence in fragments shorter than    2336 bp
  50% of sequence in fragments shorter than    2603 bp
  55% of sequence in fragments shorter than    2894 bp
  60% of sequence in fragments shorter than    3209 bp
  65% of sequence in fragments shorter than    3562 bp
  70% of sequence in fragments shorter than    3949 bp
  75% of sequence in fragments shorter than    4389 bp
  80% of sequence in fragments shorter than    4952 bp
  85% of sequence in fragments shorter than    5674 bp
  90% of sequence in fragments shorter than    6719 bp
  95% of sequence in fragments shorter than    8646 bp

# BspHI.BspMI (TCATGA+ACCTGC) double digest, 2415 thousand fragments (978290+1438129 sites), 1233 bp average fragment length
   5% of sequence in fragments shorter than     431 bp
  10% of sequence in fragments shorter than     661 bp
  15% of sequence in fragments shorter than     853 bp
  20% of sequence in fragments shorter than    1026 bp
  25% of sequence in fragments shorter than    1206 bp
  30% of sequence in fragments shorter than    1383 bp
  35% of sequence in fragments shorter than    1553 bp
  40% of sequence in fragments shorter than    1738 bp
  45% of sequence in fragments shorter than    1922 bp
  50% of sequence in fragments shorter than    2111 bp
  55% of sequence in fragments shorter than    2330 bp
  60% of sequence in fragments shorter than    2573 bp
  65% of sequence in fragments shorter than    2837 bp
  70% of sequence in fragments shorter than    3136 bp
  75% of sequence in fragments shorter than    3481 bp
  80% of sequence in fragments shorter than    3896 bp
  85% of sequence in fragments shorter than    4430 bp
  90% of sequence in fragments shorter than    5179 bp
  95% of sequence in fragments shorter than    6496 bp

# BceAI.BfiI (ACGGC+ACTGGG) double digest, 2411 thousand fragments (705178+1706594 sites), 1235 bp average fragment length
   5% of sequence in fragments shorter than     404 bp
  10% of sequence in fragments shorter than     642 bp
  15% of sequence in fragments shorter than     851 bp
  20% of sequence in fragments shorter than    1047 bp
  25% of sequence in fragments shorter than    1248 bp
  30% of sequence in fragments shorter than    1452 bp
  35% of sequence in fragments shorter than    1663 bp
  40% of sequence in fragments shorter than    1879 bp
  45% of sequence in fragments shorter than    2102 bp
  50% of sequence in fragments shorter than    2340 bp
  55% of sequence in fragments shorter than    2608 bp
  60% of sequence in fragments shorter than    2877 bp
  65% of sequence in fragments shorter than    3206 bp
  70% of sequence in fragments shorter than    3580 bp
  75% of sequence in fragments shorter than    4025 bp
  80% of sequence in fragments shorter than    4557 bp
  85% of sequence in fragments shorter than    5235 bp
  90% of sequence in fragments shorter than    6226 bp
  95% of sequence in fragments shorter than    8087 bp

# HgiCI.HindIII (GGYRCC+AAGCTT) double digest, 2410 thousand fragments (1550877+860365 sites), 1236 bp average fragment length
   5% of sequence in fragments shorter than     418 bp
  10% of sequence in fragments shorter than     642 bp
  15% of sequence in fragments shorter than     840 bp
  20% of sequence in fragments shorter than    1019 bp
  25% of sequence in fragments shorter than    1198 bp
  30% of sequence in fragments shorter than    1380 bp
  35% of sequence in fragments shorter than    1570 bp
  40% of sequence in fragments shorter than    1750 bp
  45% of sequence in fragments shorter than    1951 bp
  50% of sequence in fragments shorter than    2172 bp
  55% of sequence in fragments shorter than    2409 bp
  60% of sequence in fragments shorter than    2665 bp
  65% of sequence in fragments shorter than    2945 bp
  70% of sequence in fragments shorter than    3265 bp
  75% of sequence in fragments shorter than    3646 bp
  80% of sequence in fragments shorter than    4111 bp
  85% of sequence in fragments shorter than    4707 bp
  90% of sequence in fragments shorter than    5570 bp
  95% of sequence in fragments shorter than    7199 bp

# AflIII.StuI (ACRYGT+AGGCCT) double digest, 2410 thousand fragments (1485394+925729 sites), 1236 bp average fragment length
   5% of sequence in fragments shorter than     411 bp
  10% of sequence in fragments shorter than     664 bp
  15% of sequence in fragments shorter than     859 bp
  20% of sequence in fragments shorter than    1054 bp
  25% of sequence in fragments shorter than    1249 bp
  30% of sequence in fragments shorter than    1440 bp
  35% of sequence in fragments shorter than    1638 bp
  40% of sequence in fragments shorter than    1835 bp
  45% of sequence in fragments shorter than    2034 bp
  50% of sequence in fragments shorter than    2255 bp
  55% of sequence in fragments shorter than    2497 bp
  60% of sequence in fragments shorter than    2757 bp
  65% of sequence in fragments shorter than    3040 bp
  70% of sequence in fragments shorter than    3362 bp
  75% of sequence in fragments shorter than    3731 bp
  80% of sequence in fragments shorter than    4162 bp
  85% of sequence in fragments shorter than    4718 bp
  90% of sequence in fragments shorter than    5486 bp
  95% of sequence in fragments shorter than    6792 bp

# BsmI.HindIII (GAATGC+AAGCTT) double digest, 2409 thousand fragments (1549350+860365 sites), 1236 bp average fragment length
   5% of sequence in fragments shorter than     393 bp
  10% of sequence in fragments shorter than     683 bp
  15% of sequence in fragments shorter than     918 bp
  20% of sequence in fragments shorter than    1131 bp
  25% of sequence in fragments shorter than    1343 bp
  30% of sequence in fragments shorter than    1552 bp
  35% of sequence in fragments shorter than    1765 bp
  40% of sequence in fragments shorter than    1969 bp
  45% of sequence in fragments shorter than    2191 bp
  50% of sequence in fragments shorter than    2424 bp
  55% of sequence in fragments shorter than    2674 bp
  60% of sequence in fragments shorter than    2947 bp
  65% of sequence in fragments shorter than    3249 bp
  70% of sequence in fragments shorter than    3591 bp
  75% of sequence in fragments shorter than    3984 bp
  80% of sequence in fragments shorter than    4455 bp
  85% of sequence in fragments shorter than    5049 bp
  90% of sequence in fragments shorter than    5888 bp
  95% of sequence in fragments shorter than    7290 bp

# SalI.SspI (GTCGAC+AATATT) double digest, 2408 thousand fragments (31396+2377267 sites), 1237 bp average fragment length
   5% of sequence in fragments shorter than     404 bp
  10% of sequence in fragments shorter than     637 bp
  15% of sequence in fragments shorter than     844 bp
  20% of sequence in fragments shorter than    1048 bp
  25% of sequence in fragments shorter than    1254 bp
  30% of sequence in fragments shorter than    1458 bp
  35% of sequence in fragments shorter than    1678 bp
  40% of sequence in fragments shorter than    1912 bp
  45% of sequence in fragments shorter than    2164 bp
  50% of sequence in fragments shorter than    2439 bp
  55% of sequence in fragments shorter than    2753 bp
  60% of sequence in fragments shorter than    3106 bp
  65% of sequence in fragments shorter than    3515 bp
  70% of sequence in fragments shorter than    4004 bp
  75% of sequence in fragments shorter than    4577 bp
  80% of sequence in fragments shorter than    5329 bp
  85% of sequence in fragments shorter than    6430 bp
  90% of sequence in fragments shorter than    8281 bp
  95% of sequence in fragments shorter than   12762 bp

# NcoI.PsiI (CCATGG+TTATAA) double digest, 2407 thousand fragments (759594+1647912 sites), 1238 bp average fragment length
   5% of sequence in fragments shorter than     425 bp
  10% of sequence in fragments shorter than     655 bp
  15% of sequence in fragments shorter than     856 bp
  20% of sequence in fragments shorter than    1044 bp
  25% of sequence in fragments shorter than    1230 bp
  30% of sequence in fragments shorter than    1410 bp
  35% of sequence in fragments shorter than    1598 bp
  40% of sequence in fragments shorter than    1798 bp
  45% of sequence in fragments shorter than    2007 bp
  50% of sequence in fragments shorter than    2230 bp
  55% of sequence in fragments shorter than    2471 bp
  60% of sequence in fragments shorter than    2739 bp
  65% of sequence in fragments shorter than    3018 bp
  70% of sequence in fragments shorter than    3333 bp
  75% of sequence in fragments shorter than    3730 bp
  80% of sequence in fragments shorter than    4215 bp
  85% of sequence in fragments shorter than    4853 bp
  90% of sequence in fragments shorter than    5781 bp
  95% of sequence in fragments shorter than    7629 bp

# PpuMI.PstI (RGGWCCY+CTGCAG) double digest, 2406 thousand fragments (1085138+1321469 sites), 1238 bp average fragment length
   5% of sequence in fragments shorter than     400 bp
  10% of sequence in fragments shorter than     646 bp
  15% of sequence in fragments shorter than     862 bp
  20% of sequence in fragments shorter than    1080 bp
  25% of sequence in fragments shorter than    1298 bp
  30% of sequence in fragments shorter than    1520 bp
  35% of sequence in fragments shorter than    1756 bp
  40% of sequence in fragments shorter than    1999 bp
  45% of sequence in fragments shorter than    2252 bp
  50% of sequence in fragments shorter than    2532 bp
  55% of sequence in fragments shorter than    2831 bp
  60% of sequence in fragments shorter than    3140 bp
  65% of sequence in fragments shorter than    3511 bp
  70% of sequence in fragments shorter than    3934 bp
  75% of sequence in fragments shorter than    4443 bp
  80% of sequence in fragments shorter than    5071 bp
  85% of sequence in fragments shorter than    5875 bp
  90% of sequence in fragments shorter than    7056 bp
  95% of sequence in fragments shorter than    9263 bp

# PstI.PvuII (CTGCAG+CAGCTG) double digest, 2405 thousand fragments (1321469+1084260 sites), 1238 bp average fragment length
   5% of sequence in fragments shorter than     418 bp
  10% of sequence in fragments shorter than     661 bp
  15% of sequence in fragments shorter than     878 bp
  20% of sequence in fragments shorter than    1089 bp
  25% of sequence in fragments shorter than    1303 bp
  30% of sequence in fragments shorter than    1517 bp
  35% of sequence in fragments shorter than    1745 bp
  40% of sequence in fragments shorter than    1983 bp
  45% of sequence in fragments shorter than    2231 bp
  50% of sequence in fragments shorter than    2507 bp
  55% of sequence in fragments shorter than    2804 bp
  60% of sequence in fragments shorter than    3135 bp
  65% of sequence in fragments shorter than    3508 bp
  70% of sequence in fragments shorter than    3942 bp
  75% of sequence in fragments shorter than    4463 bp
  80% of sequence in fragments shorter than    5079 bp
  85% of sequence in fragments shorter than    5867 bp
  90% of sequence in fragments shorter than    6998 bp
  95% of sequence in fragments shorter than    9103 bp

# ClaI.HpaII (ATCGAT+CCGG) double digest, 2403 thousand fragments (86477+2317722 sites), 1239 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     699 bp
  15% of sequence in fragments shorter than     994 bp
  20% of sequence in fragments shorter than    1290 bp
  25% of sequence in fragments shorter than    1595 bp
  30% of sequence in fragments shorter than    1912 bp
  35% of sequence in fragments shorter than    2252 bp
  40% of sequence in fragments shorter than    2624 bp
  45% of sequence in fragments shorter than    3024 bp
  50% of sequence in fragments shorter than    3461 bp
  55% of sequence in fragments shorter than    3949 bp
  60% of sequence in fragments shorter than    4493 bp
  65% of sequence in fragments shorter than    5114 bp
  70% of sequence in fragments shorter than    5829 bp
  75% of sequence in fragments shorter than    6680 bp
  80% of sequence in fragments shorter than    7741 bp
  85% of sequence in fragments shorter than    9152 bp
  90% of sequence in fragments shorter than   11239 bp
  95% of sequence in fragments shorter than   15330 bp

# BciVI.BfiI (GTATCC+ACTGGG) double digest, 2402 thousand fragments (696093+1706594 sites), 1240 bp average fragment length
   5% of sequence in fragments shorter than     423 bp
  10% of sequence in fragments shorter than     657 bp
  15% of sequence in fragments shorter than     856 bp
  20% of sequence in fragments shorter than    1039 bp
  25% of sequence in fragments shorter than    1216 bp
  30% of sequence in fragments shorter than    1390 bp
  35% of sequence in fragments shorter than    1568 bp
  40% of sequence in fragments shorter than    1757 bp
  45% of sequence in fragments shorter than    1949 bp
  50% of sequence in fragments shorter than    2162 bp
  55% of sequence in fragments shorter than    2386 bp
  60% of sequence in fragments shorter than    2635 bp
  65% of sequence in fragments shorter than    2916 bp
  70% of sequence in fragments shorter than    3231 bp
  75% of sequence in fragments shorter than    3600 bp
  80% of sequence in fragments shorter than    4045 bp
  85% of sequence in fragments shorter than    4619 bp
  90% of sequence in fragments shorter than    5440 bp
  95% of sequence in fragments shorter than    6935 bp

# HgiCI.XbaI (GGYRCC+TCTAGA) double digest, 2401 thousand fragments (1550877+850998 sites), 1240 bp average fragment length
   5% of sequence in fragments shorter than     424 bp
  10% of sequence in fragments shorter than     648 bp
  15% of sequence in fragments shorter than     831 bp
  20% of sequence in fragments shorter than    1013 bp
  25% of sequence in fragments shorter than    1195 bp
  30% of sequence in fragments shorter than    1373 bp
  35% of sequence in fragments shorter than    1547 bp
  40% of sequence in fragments shorter than    1740 bp
  45% of sequence in fragments shorter than    1943 bp
  50% of sequence in fragments shorter than    2161 bp
  55% of sequence in fragments shorter than    2396 bp
  60% of sequence in fragments shorter than    2649 bp
  65% of sequence in fragments shorter than    2922 bp
  70% of sequence in fragments shorter than    3244 bp
  75% of sequence in fragments shorter than    3618 bp
  80% of sequence in fragments shorter than    4072 bp
  85% of sequence in fragments shorter than    4652 bp
  90% of sequence in fragments shorter than    5477 bp
  95% of sequence in fragments shorter than    6952 bp

# BsmI.XbaI (GAATGC+TCTAGA) double digest, 2399 thousand fragments (1549350+850998 sites), 1241 bp average fragment length
   5% of sequence in fragments shorter than     418 bp
  10% of sequence in fragments shorter than     702 bp
  15% of sequence in fragments shorter than     942 bp
  20% of sequence in fragments shorter than    1159 bp
  25% of sequence in fragments shorter than    1357 bp
  30% of sequence in fragments shorter than    1566 bp
  35% of sequence in fragments shorter than    1774 bp
  40% of sequence in fragments shorter than    1994 bp
  45% of sequence in fragments shorter than    2219 bp
  50% of sequence in fragments shorter than    2458 bp
  55% of sequence in fragments shorter than    2715 bp
  60% of sequence in fragments shorter than    2992 bp
  65% of sequence in fragments shorter than    3299 bp
  70% of sequence in fragments shorter than    3646 bp
  75% of sequence in fragments shorter than    4049 bp
  80% of sequence in fragments shorter than    4532 bp
  85% of sequence in fragments shorter than    5145 bp
  90% of sequence in fragments shorter than    5998 bp
  95% of sequence in fragments shorter than    7445 bp

# AflII.HhaI (CTTAAG+GCGC) double digest, 2399 thousand fragments (643284+1756502 sites), 1242 bp average fragment length
   5% of sequence in fragments shorter than     422 bp
  10% of sequence in fragments shorter than     698 bp
  15% of sequence in fragments shorter than     966 bp
  20% of sequence in fragments shorter than    1213 bp
  25% of sequence in fragments shorter than    1464 bp
  30% of sequence in fragments shorter than    1722 bp
  35% of sequence in fragments shorter than    1971 bp
  40% of sequence in fragments shorter than    2245 bp
  45% of sequence in fragments shorter than    2529 bp
  50% of sequence in fragments shorter than    2837 bp
  55% of sequence in fragments shorter than    3172 bp
  60% of sequence in fragments shorter than    3529 bp
  65% of sequence in fragments shorter than    3926 bp
  70% of sequence in fragments shorter than    4386 bp
  75% of sequence in fragments shorter than    4929 bp
  80% of sequence in fragments shorter than    5579 bp
  85% of sequence in fragments shorter than    6407 bp
  90% of sequence in fragments shorter than    7582 bp
  95% of sequence in fragments shorter than    9604 bp

# MluI.SspI (ACGCGT+AATATT) double digest, 2398 thousand fragments (21415+2377267 sites), 1242 bp average fragment length
   5% of sequence in fragments shorter than     406 bp
  10% of sequence in fragments shorter than     640 bp
  15% of sequence in fragments shorter than     848 bp
  20% of sequence in fragments shorter than    1053 bp
  25% of sequence in fragments shorter than    1260 bp
  30% of sequence in fragments shorter than    1466 bp
  35% of sequence in fragments shorter than    1688 bp
  40% of sequence in fragments shorter than    1923 bp
  45% of sequence in fragments shorter than    2177 bp
  50% of sequence in fragments shorter than    2453 bp
  55% of sequence in fragments shorter than    2767 bp
  60% of sequence in fragments shorter than    3122 bp
  65% of sequence in fragments shorter than    3532 bp
  70% of sequence in fragments shorter than    4022 bp
  75% of sequence in fragments shorter than    4598 bp
  80% of sequence in fragments shorter than    5353 bp
  85% of sequence in fragments shorter than    6443 bp
  90% of sequence in fragments shorter than    8275 bp
  95% of sequence in fragments shorter than   12642 bp

# EcoRI.HgiCI (GAATTC+GGYRCC) double digest, 2397 thousand fragments (847401+1550877 sites), 1242 bp average fragment length
   5% of sequence in fragments shorter than     413 bp
  10% of sequence in fragments shorter than     648 bp
  15% of sequence in fragments shorter than     838 bp
  20% of sequence in fragments shorter than    1033 bp
  25% of sequence in fragments shorter than    1217 bp
  30% of sequence in fragments shorter than    1390 bp
  35% of sequence in fragments shorter than    1575 bp
  40% of sequence in fragments shorter than    1774 bp
  45% of sequence in fragments shorter than    1983 bp
  50% of sequence in fragments shorter than    2207 bp
  55% of sequence in fragments shorter than    2445 bp
  60% of sequence in fragments shorter than    2706 bp
  65% of sequence in fragments shorter than    2981 bp
  70% of sequence in fragments shorter than    3306 bp
  75% of sequence in fragments shorter than    3689 bp
  80% of sequence in fragments shorter than    4158 bp
  85% of sequence in fragments shorter than    4750 bp
  90% of sequence in fragments shorter than    5607 bp
  95% of sequence in fragments shorter than    7138 bp

# BsmI.EcoRI (GAATGC+GAATTC) double digest, 2396 thousand fragments (1549350+847401 sites), 1243 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     679 bp
  15% of sequence in fragments shorter than     921 bp
  20% of sequence in fragments shorter than    1148 bp
  25% of sequence in fragments shorter than    1365 bp
  30% of sequence in fragments shorter than    1581 bp
  35% of sequence in fragments shorter than    1798 bp
  40% of sequence in fragments shorter than    2016 bp
  45% of sequence in fragments shorter than    2242 bp
  50% of sequence in fragments shorter than    2480 bp
  55% of sequence in fragments shorter than    2734 bp
  60% of sequence in fragments shorter than    3011 bp
  65% of sequence in fragments shorter than    3315 bp
  70% of sequence in fragments shorter than    3664 bp
  75% of sequence in fragments shorter than    4063 bp
  80% of sequence in fragments shorter than    4542 bp
  85% of sequence in fragments shorter than    5145 bp
  90% of sequence in fragments shorter than    5981 bp
  95% of sequence in fragments shorter than    7413 bp

# SgrAI.SspI (CRCCGGYG+AATATT) double digest, 2392 thousand fragments (15270+2377267 sites), 1245 bp average fragment length
   5% of sequence in fragments shorter than     407 bp
  10% of sequence in fragments shorter than     642 bp
  15% of sequence in fragments shorter than     851 bp
  20% of sequence in fragments shorter than    1056 bp
  25% of sequence in fragments shorter than    1264 bp
  30% of sequence in fragments shorter than    1470 bp
  35% of sequence in fragments shorter than    1692 bp
  40% of sequence in fragments shorter than    1927 bp
  45% of sequence in fragments shorter than    2181 bp
  50% of sequence in fragments shorter than    2458 bp
  55% of sequence in fragments shorter than    2772 bp
  60% of sequence in fragments shorter than    3129 bp
  65% of sequence in fragments shorter than    3540 bp
  70% of sequence in fragments shorter than    4030 bp
  75% of sequence in fragments shorter than    4607 bp
  80% of sequence in fragments shorter than    5366 bp
  85% of sequence in fragments shorter than    6466 bp
  90% of sequence in fragments shorter than    8325 bp
  95% of sequence in fragments shorter than   12768 bp

# NruI.SspI (TCGCGA+AATATT) double digest, 2391 thousand fragments (14872+2377267 sites), 1245 bp average fragment length
   5% of sequence in fragments shorter than     407 bp
  10% of sequence in fragments shorter than     641 bp
  15% of sequence in fragments shorter than     850 bp
  20% of sequence in fragments shorter than    1055 bp
  25% of sequence in fragments shorter than    1263 bp
  30% of sequence in fragments shorter than    1470 bp
  35% of sequence in fragments shorter than    1692 bp
  40% of sequence in fragments shorter than    1928 bp
  45% of sequence in fragments shorter than    2183 bp
  50% of sequence in fragments shorter than    2460 bp
  55% of sequence in fragments shorter than    2776 bp
  60% of sequence in fragments shorter than    3134 bp
  65% of sequence in fragments shorter than    3549 bp
  70% of sequence in fragments shorter than    4041 bp
  75% of sequence in fragments shorter than    4622 bp
  80% of sequence in fragments shorter than    5392 bp
  85% of sequence in fragments shorter than    6511 bp
  90% of sequence in fragments shorter than    8431 bp
  95% of sequence in fragments shorter than   13124 bp

# PvuI.SspI (CGATCG+AATATT) double digest, 2391 thousand fragments (14186+2377267 sites), 1246 bp average fragment length
   5% of sequence in fragments shorter than     407 bp
  10% of sequence in fragments shorter than     641 bp
  15% of sequence in fragments shorter than     850 bp
  20% of sequence in fragments shorter than    1056 bp
  25% of sequence in fragments shorter than    1264 bp
  30% of sequence in fragments shorter than    1471 bp
  35% of sequence in fragments shorter than    1694 bp
  40% of sequence in fragments shorter than    1928 bp
  45% of sequence in fragments shorter than    2183 bp
  50% of sequence in fragments shorter than    2461 bp
  55% of sequence in fragments shorter than    2777 bp
  60% of sequence in fragments shorter than    3135 bp
  65% of sequence in fragments shorter than    3549 bp
  70% of sequence in fragments shorter than    4042 bp
  75% of sequence in fragments shorter than    4624 bp
  80% of sequence in fragments shorter than    5389 bp
  85% of sequence in fragments shorter than    6501 bp
  90% of sequence in fragments shorter than    8402 bp
  95% of sequence in fragments shorter than   13048 bp

# FseI.SspI (GGCCGGCC+AATATT) double digest, 2390 thousand fragments (13662+2377267 sites), 1246 bp average fragment length
   5% of sequence in fragments shorter than     407 bp
  10% of sequence in fragments shorter than     642 bp
  15% of sequence in fragments shorter than     851 bp
  20% of sequence in fragments shorter than    1057 bp
  25% of sequence in fragments shorter than    1265 bp
  30% of sequence in fragments shorter than    1472 bp
  35% of sequence in fragments shorter than    1694 bp
  40% of sequence in fragments shorter than    1930 bp
  45% of sequence in fragments shorter than    2185 bp
  50% of sequence in fragments shorter than    2462 bp
  55% of sequence in fragments shorter than    2777 bp
  60% of sequence in fragments shorter than    3134 bp
  65% of sequence in fragments shorter than    3546 bp
  70% of sequence in fragments shorter than    4036 bp
  75% of sequence in fragments shorter than    4612 bp
  80% of sequence in fragments shorter than    5364 bp
  85% of sequence in fragments shorter than    6457 bp
  90% of sequence in fragments shorter than    8281 bp
  95% of sequence in fragments shorter than   12566 bp

# AatII.HpaII (GACGTC+CCGG) double digest, 2388 thousand fragments (71089+2317722 sites), 1247 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     703 bp
  15% of sequence in fragments shorter than    1005 bp
  20% of sequence in fragments shorter than    1310 bp
  25% of sequence in fragments shorter than    1627 bp
  30% of sequence in fragments shorter than    1958 bp
  35% of sequence in fragments shorter than    2314 bp
  40% of sequence in fragments shorter than    2707 bp
  45% of sequence in fragments shorter than    3130 bp
  50% of sequence in fragments shorter than    3595 bp
  55% of sequence in fragments shorter than    4110 bp
  60% of sequence in fragments shorter than    4692 bp
  65% of sequence in fragments shorter than    5355 bp
  70% of sequence in fragments shorter than    6112 bp
  75% of sequence in fragments shorter than    7036 bp
  80% of sequence in fragments shorter than    8185 bp
  85% of sequence in fragments shorter than    9703 bp
  90% of sequence in fragments shorter than   11934 bp
  95% of sequence in fragments shorter than   16267 bp

# AflIII.NdeI (ACRYGT+CATATG) double digest, 2388 thousand fragments (1485394+903360 sites), 1247 bp average fragment length
   5% of sequence in fragments shorter than     430 bp
  10% of sequence in fragments shorter than     685 bp
  15% of sequence in fragments shorter than     885 bp
  20% of sequence in fragments shorter than    1088 bp
  25% of sequence in fragments shorter than    1286 bp
  30% of sequence in fragments shorter than    1489 bp
  35% of sequence in fragments shorter than    1695 bp
  40% of sequence in fragments shorter than    1905 bp
  45% of sequence in fragments shorter than    2124 bp
  50% of sequence in fragments shorter than    2359 bp
  55% of sequence in fragments shorter than    2614 bp
  60% of sequence in fragments shorter than    2887 bp
  65% of sequence in fragments shorter than    3195 bp
  70% of sequence in fragments shorter than    3550 bp
  75% of sequence in fragments shorter than    3970 bp
  80% of sequence in fragments shorter than    4484 bp
  85% of sequence in fragments shorter than    5126 bp
  90% of sequence in fragments shorter than    6037 bp
  95% of sequence in fragments shorter than    7806 bp

# NotI.SspI (GCGGCCGC+AATATT) double digest, 2386 thousand fragments (9795+2377267 sites), 1248 bp average fragment length
   5% of sequence in fragments shorter than     408 bp
  10% of sequence in fragments shorter than     643 bp
  15% of sequence in fragments shorter than     852 bp
  20% of sequence in fragments shorter than    1058 bp
  25% of sequence in fragments shorter than    1267 bp
  30% of sequence in fragments shorter than    1474 bp
  35% of sequence in fragments shorter than    1697 bp
  40% of sequence in fragments shorter than    1934 bp
  45% of sequence in fragments shorter than    2189 bp
  50% of sequence in fragments shorter than    2468 bp
  55% of sequence in fragments shorter than    2784 bp
  60% of sequence in fragments shorter than    3144 bp
  65% of sequence in fragments shorter than    3559 bp
  70% of sequence in fragments shorter than    4054 bp
  75% of sequence in fragments shorter than    4636 bp
  80% of sequence in fragments shorter than    5407 bp
  85% of sequence in fragments shorter than    6524 bp
  90% of sequence in fragments shorter than    8423 bp
  95% of sequence in fragments shorter than   13014 bp

# RsrII.SspI (CGGWCCG+AATATT) double digest, 2386 thousand fragments (9764+2377267 sites), 1248 bp average fragment length
   5% of sequence in fragments shorter than     408 bp
  10% of sequence in fragments shorter than     643 bp
  15% of sequence in fragments shorter than     852 bp
  20% of sequence in fragments shorter than    1057 bp
  25% of sequence in fragments shorter than    1266 bp
  30% of sequence in fragments shorter than    1474 bp
  35% of sequence in fragments shorter than    1697 bp
  40% of sequence in fragments shorter than    1934 bp
  45% of sequence in fragments shorter than    2189 bp
  50% of sequence in fragments shorter than    2468 bp
  55% of sequence in fragments shorter than    2784 bp
  60% of sequence in fragments shorter than    3144 bp
  65% of sequence in fragments shorter than    3560 bp
  70% of sequence in fragments shorter than    4055 bp
  75% of sequence in fragments shorter than    4637 bp
  80% of sequence in fragments shorter than    5406 bp
  85% of sequence in fragments shorter than    6527 bp
  90% of sequence in fragments shorter than    8437 bp
  95% of sequence in fragments shorter than   13029 bp

# BclI.PsiI (TGATCA+TTATAA) double digest, 2386 thousand fragments (738785+1647912 sites), 1248 bp average fragment length
   5% of sequence in fragments shorter than     422 bp
  10% of sequence in fragments shorter than     651 bp
  15% of sequence in fragments shorter than     854 bp
  20% of sequence in fragments shorter than    1036 bp
  25% of sequence in fragments shorter than    1227 bp
  30% of sequence in fragments shorter than    1418 bp
  35% of sequence in fragments shorter than    1616 bp
  40% of sequence in fragments shorter than    1820 bp
  45% of sequence in fragments shorter than    2032 bp
  50% of sequence in fragments shorter than    2272 bp
  55% of sequence in fragments shorter than    2534 bp
  60% of sequence in fragments shorter than    2821 bp
  65% of sequence in fragments shorter than    3141 bp
  70% of sequence in fragments shorter than    3523 bp
  75% of sequence in fragments shorter than    3992 bp
  80% of sequence in fragments shorter than    4588 bp
  85% of sequence in fragments shorter than    5421 bp
  90% of sequence in fragments shorter than    6751 bp
  95% of sequence in fragments shorter than    9878 bp

# HaeII.HhaI (RGCGCY+GCGC) double digest, 2386 thousand fragments (630101+1756502 sites), 1248 bp average fragment length
   5% of sequence in fragments shorter than     570 bp
  10% of sequence in fragments shorter than    1006 bp
  15% of sequence in fragments shorter than    1439 bp
  20% of sequence in fragments shorter than    1881 bp
  25% of sequence in fragments shorter than    2351 bp
  30% of sequence in fragments shorter than    2842 bp
  35% of sequence in fragments shorter than    3373 bp
  40% of sequence in fragments shorter than    3941 bp
  45% of sequence in fragments shorter than    4553 bp
  50% of sequence in fragments shorter than    5231 bp
  55% of sequence in fragments shorter than    5980 bp
  60% of sequence in fragments shorter than    6818 bp
  65% of sequence in fragments shorter than    7774 bp
  70% of sequence in fragments shorter than    8880 bp
  75% of sequence in fragments shorter than   10218 bp
  80% of sequence in fragments shorter than   11862 bp
  85% of sequence in fragments shorter than   13974 bp
  90% of sequence in fragments shorter than   17029 bp
  95% of sequence in fragments shorter than   22360 bp

# HpaII.SacII (CCGG+CCGCGG) double digest, 2384 thousand fragments (2317722+66901 sites), 1249 bp average fragment length
   5% of sequence in fragments shorter than     391 bp
  10% of sequence in fragments shorter than     718 bp
  15% of sequence in fragments shorter than    1031 bp
  20% of sequence in fragments shorter than    1346 bp
  25% of sequence in fragments shorter than    1674 bp
  30% of sequence in fragments shorter than    2019 bp
  35% of sequence in fragments shorter than    2389 bp
  40% of sequence in fragments shorter than    2799 bp
  45% of sequence in fragments shorter than    3242 bp
  50% of sequence in fragments shorter than    3727 bp
  55% of sequence in fragments shorter than    4266 bp
  60% of sequence in fragments shorter than    4879 bp
  65% of sequence in fragments shorter than    5574 bp
  70% of sequence in fragments shorter than    6366 bp
  75% of sequence in fragments shorter than    7336 bp
  80% of sequence in fragments shorter than    8548 bp
  85% of sequence in fragments shorter than   10139 bp
  90% of sequence in fragments shorter than   12491 bp
  95% of sequence in fragments shorter than   17224 bp

# AscI.SspI (GGCGCGCC+AATATT) double digest, 2381 thousand fragments (4751+2377267 sites), 1251 bp average fragment length
   5% of sequence in fragments shorter than     408 bp
  10% of sequence in fragments shorter than     643 bp
  15% of sequence in fragments shorter than     853 bp
  20% of sequence in fragments shorter than    1059 bp
  25% of sequence in fragments shorter than    1269 bp
  30% of sequence in fragments shorter than    1477 bp
  35% of sequence in fragments shorter than    1701 bp
  40% of sequence in fragments shorter than    1939 bp
  45% of sequence in fragments shorter than    2196 bp
  50% of sequence in fragments shorter than    2476 bp
  55% of sequence in fragments shorter than    2795 bp
  60% of sequence in fragments shorter than    3157 bp
  65% of sequence in fragments shorter than    3576 bp
  70% of sequence in fragments shorter than    4076 bp
  75% of sequence in fragments shorter than    4664 bp
  80% of sequence in fragments shorter than    5449 bp
  85% of sequence in fragments shorter than    6594 bp
  90% of sequence in fragments shorter than    8571 bp
  95% of sequence in fragments shorter than   13461 bp

# FnuDII.PsiI (CGCG+TTATAA) double digest, 2381 thousand fragments (733940+1647912 sites), 1251 bp average fragment length
   5% of sequence in fragments shorter than     443 bp
  10% of sequence in fragments shorter than     709 bp
  15% of sequence in fragments shorter than     942 bp
  20% of sequence in fragments shorter than    1162 bp
  25% of sequence in fragments shorter than    1378 bp
  30% of sequence in fragments shorter than    1596 bp
  35% of sequence in fragments shorter than    1818 bp
  40% of sequence in fragments shorter than    2045 bp
  45% of sequence in fragments shorter than    2290 bp
  50% of sequence in fragments shorter than    2544 bp
  55% of sequence in fragments shorter than    2825 bp
  60% of sequence in fragments shorter than    3114 bp
  65% of sequence in fragments shorter than    3456 bp
  70% of sequence in fragments shorter than    3844 bp
  75% of sequence in fragments shorter than    4302 bp
  80% of sequence in fragments shorter than    4873 bp
  85% of sequence in fragments shorter than    5619 bp
  90% of sequence in fragments shorter than    6715 bp
  95% of sequence in fragments shorter than    8863 bp

# BglII.EsaBC3I (AGATCT+TCGA) double digest, 2377 thousand fragments (774732+1603346 sites), 1253 bp average fragment length
   5% of sequence in fragments shorter than     436 bp
  10% of sequence in fragments shorter than     658 bp
  15% of sequence in fragments shorter than     851 bp
  20% of sequence in fragments shorter than    1034 bp
  25% of sequence in fragments shorter than    1211 bp
  30% of sequence in fragments shorter than    1386 bp
  35% of sequence in fragments shorter than    1571 bp
  40% of sequence in fragments shorter than    1762 bp
  45% of sequence in fragments shorter than    1962 bp
  50% of sequence in fragments shorter than    2176 bp
  55% of sequence in fragments shorter than    2405 bp
  60% of sequence in fragments shorter than    2653 bp
  65% of sequence in fragments shorter than    2927 bp
  70% of sequence in fragments shorter than    3228 bp
  75% of sequence in fragments shorter than    3585 bp
  80% of sequence in fragments shorter than    4012 bp
  85% of sequence in fragments shorter than    4557 bp
  90% of sequence in fragments shorter than    5312 bp
  95% of sequence in fragments shorter than    6634 bp

# HpaII.PspXI (CCGG+VCTCGAGB) double digest, 2376 thousand fragments (2317722+59193 sites), 1253 bp average fragment length
   5% of sequence in fragments shorter than     387 bp
  10% of sequence in fragments shorter than     706 bp
  15% of sequence in fragments shorter than    1010 bp
  20% of sequence in fragments shorter than    1318 bp
  25% of sequence in fragments shorter than    1636 bp
  30% of sequence in fragments shorter than    1971 bp
  35% of sequence in fragments shorter than    2331 bp
  40% of sequence in fragments shorter than    2730 bp
  45% of sequence in fragments shorter than    3159 bp
  50% of sequence in fragments shorter than    3632 bp
  55% of sequence in fragments shorter than    4155 bp
  60% of sequence in fragments shorter than    4748 bp
  65% of sequence in fragments shorter than    5421 bp
  70% of sequence in fragments shorter than    6197 bp
  75% of sequence in fragments shorter than    7133 bp
  80% of sequence in fragments shorter than    8302 bp
  85% of sequence in fragments shorter than    9845 bp
  90% of sequence in fragments shorter than   12108 bp
  95% of sequence in fragments shorter than   16647 bp

# AvaIII.FauI (ATGCAT+CCCGC) double digest, 2372 thousand fragments (933357+1439772 sites), 1255 bp average fragment length
   5% of sequence in fragments shorter than     442 bp
  10% of sequence in fragments shorter than     720 bp
  15% of sequence in fragments shorter than     961 bp
  20% of sequence in fragments shorter than    1193 bp
  25% of sequence in fragments shorter than    1414 bp
  30% of sequence in fragments shorter than    1645 bp
  35% of sequence in fragments shorter than    1882 bp
  40% of sequence in fragments shorter than    2127 bp
  45% of sequence in fragments shorter than    2386 bp
  50% of sequence in fragments shorter than    2658 bp
  55% of sequence in fragments shorter than    2955 bp
  60% of sequence in fragments shorter than    3284 bp
  65% of sequence in fragments shorter than    3650 bp
  70% of sequence in fragments shorter than    4066 bp
  75% of sequence in fragments shorter than    4535 bp
  80% of sequence in fragments shorter than    5129 bp
  85% of sequence in fragments shorter than    5880 bp
  90% of sequence in fragments shorter than    6970 bp
  95% of sequence in fragments shorter than    8965 bp

# AvaIII.BspMI (ATGCAT+ACCTGC) double digest, 2371 thousand fragments (933357+1438129 sites), 1256 bp average fragment length
   5% of sequence in fragments shorter than     433 bp
  10% of sequence in fragments shorter than     670 bp
  15% of sequence in fragments shorter than     864 bp
  20% of sequence in fragments shorter than    1053 bp
  25% of sequence in fragments shorter than    1239 bp
  30% of sequence in fragments shorter than    1421 bp
  35% of sequence in fragments shorter than    1611 bp
  40% of sequence in fragments shorter than    1808 bp
  45% of sequence in fragments shorter than    1998 bp
  50% of sequence in fragments shorter than    2212 bp
  55% of sequence in fragments shorter than    2442 bp
  60% of sequence in fragments shorter than    2691 bp
  65% of sequence in fragments shorter than    2967 bp
  70% of sequence in fragments shorter than    3267 bp
  75% of sequence in fragments shorter than    3601 bp
  80% of sequence in fragments shorter than    4031 bp
  85% of sequence in fragments shorter than    4583 bp
  90% of sequence in fragments shorter than    5333 bp
  95% of sequence in fragments shorter than    6659 bp

# AgeI.HpaII (ACCGGT+CCGG) double digest, 2370 thousand fragments (53386+2317722 sites), 1257 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     727 bp
  15% of sequence in fragments shorter than    1041 bp
  20% of sequence in fragments shorter than    1359 bp
  25% of sequence in fragments shorter than    1684 bp
  30% of sequence in fragments shorter than    2035 bp
  35% of sequence in fragments shorter than    2406 bp
  40% of sequence in fragments shorter than    2817 bp
  45% of sequence in fragments shorter than    3263 bp
  50% of sequence in fragments shorter than    3751 bp
  55% of sequence in fragments shorter than    4291 bp
  60% of sequence in fragments shorter than    4906 bp
  65% of sequence in fragments shorter than    5603 bp
  70% of sequence in fragments shorter than    6398 bp
  75% of sequence in fragments shorter than    7370 bp
  80% of sequence in fragments shorter than    8590 bp
  85% of sequence in fragments shorter than   10188 bp
  90% of sequence in fragments shorter than   12542 bp
  95% of sequence in fragments shorter than   17302 bp

# FauI.StuI (CCCGC+AGGCCT) double digest, 2365 thousand fragments (1439772+925729 sites), 1260 bp average fragment length
   5% of sequence in fragments shorter than     386 bp
  10% of sequence in fragments shorter than     689 bp
  15% of sequence in fragments shorter than     940 bp
  20% of sequence in fragments shorter than    1221 bp
  25% of sequence in fragments shorter than    1487 bp
  30% of sequence in fragments shorter than    1783 bp
  35% of sequence in fragments shorter than    2071 bp
  40% of sequence in fragments shorter than    2396 bp
  45% of sequence in fragments shorter than    2745 bp
  50% of sequence in fragments shorter than    3110 bp
  55% of sequence in fragments shorter than    3518 bp
  60% of sequence in fragments shorter than    3972 bp
  65% of sequence in fragments shorter than    4479 bp
  70% of sequence in fragments shorter than    5062 bp
  75% of sequence in fragments shorter than    5751 bp
  80% of sequence in fragments shorter than    6574 bp
  85% of sequence in fragments shorter than    7634 bp
  90% of sequence in fragments shorter than    9108 bp
  95% of sequence in fragments shorter than   11655 bp

# BspMI.StuI (ACCTGC+AGGCCT) double digest, 2363 thousand fragments (1438129+925729 sites), 1260 bp average fragment length
   5% of sequence in fragments shorter than     396 bp
  10% of sequence in fragments shorter than     652 bp
  15% of sequence in fragments shorter than     863 bp
  20% of sequence in fragments shorter than    1061 bp
  25% of sequence in fragments shorter than    1263 bp
  30% of sequence in fragments shorter than    1478 bp
  35% of sequence in fragments shorter than    1706 bp
  40% of sequence in fragments shorter than    1942 bp
  45% of sequence in fragments shorter than    2185 bp
  50% of sequence in fragments shorter than    2432 bp
  55% of sequence in fragments shorter than    2720 bp
  60% of sequence in fragments shorter than    3038 bp
  65% of sequence in fragments shorter than    3370 bp
  70% of sequence in fragments shorter than    3767 bp
  75% of sequence in fragments shorter than    4223 bp
  80% of sequence in fragments shorter than    4783 bp
  85% of sequence in fragments shorter than    5499 bp
  90% of sequence in fragments shorter than    6500 bp
  95% of sequence in fragments shorter than    8199 bp

# EsaBC3I.NcoI (TCGA+CCATGG) double digest, 2362 thousand fragments (1603346+759594 sites), 1261 bp average fragment length
   5% of sequence in fragments shorter than     435 bp
  10% of sequence in fragments shorter than     662 bp
  15% of sequence in fragments shorter than     859 bp
  20% of sequence in fragments shorter than    1047 bp
  25% of sequence in fragments shorter than    1226 bp
  30% of sequence in fragments shorter than    1407 bp
  35% of sequence in fragments shorter than    1599 bp
  40% of sequence in fragments shorter than    1796 bp
  45% of sequence in fragments shorter than    2001 bp
  50% of sequence in fragments shorter than    2218 bp
  55% of sequence in fragments shorter than    2447 bp
  60% of sequence in fragments shorter than    2707 bp
  65% of sequence in fragments shorter than    2991 bp
  70% of sequence in fragments shorter than    3315 bp
  75% of sequence in fragments shorter than    3695 bp
  80% of sequence in fragments shorter than    4144 bp
  85% of sequence in fragments shorter than    4728 bp
  90% of sequence in fragments shorter than    5563 bp
  95% of sequence in fragments shorter than    6989 bp

# BsaAI.PsiI (YACGTR+TTATAA) double digest, 2361 thousand fragments (713801+1647912 sites), 1262 bp average fragment length
   5% of sequence in fragments shorter than     435 bp
  10% of sequence in fragments shorter than     672 bp
  15% of sequence in fragments shorter than     881 bp
  20% of sequence in fragments shorter than    1072 bp
  25% of sequence in fragments shorter than    1268 bp
  30% of sequence in fragments shorter than    1457 bp
  35% of sequence in fragments shorter than    1656 bp
  40% of sequence in fragments shorter than    1863 bp
  45% of sequence in fragments shorter than    2077 bp
  50% of sequence in fragments shorter than    2312 bp
  55% of sequence in fragments shorter than    2568 bp
  60% of sequence in fragments shorter than    2842 bp
  65% of sequence in fragments shorter than    3126 bp
  70% of sequence in fragments shorter than    3474 bp
  75% of sequence in fragments shorter than    3891 bp
  80% of sequence in fragments shorter than    4404 bp
  85% of sequence in fragments shorter than    5077 bp
  90% of sequence in fragments shorter than    6070 bp
  95% of sequence in fragments shorter than    7958 bp

# HpaII.PmeI (CCGG+GTTTAAAC) double digest, 2359 thousand fragments (2317722+42094 sites), 1263 bp average fragment length
   5% of sequence in fragments shorter than     393 bp
  10% of sequence in fragments shorter than     713 bp
  15% of sequence in fragments shorter than    1017 bp
  20% of sequence in fragments shorter than    1323 bp
  25% of sequence in fragments shorter than    1639 bp
  30% of sequence in fragments shorter than    1969 bp
  35% of sequence in fragments shorter than    2324 bp
  40% of sequence in fragments shorter than    2715 bp
  45% of sequence in fragments shorter than    3134 bp
  50% of sequence in fragments shorter than    3594 bp
  55% of sequence in fragments shorter than    4106 bp
  60% of sequence in fragments shorter than    4681 bp
  65% of sequence in fragments shorter than    5336 bp
  70% of sequence in fragments shorter than    6082 bp
  75% of sequence in fragments shorter than    6987 bp
  80% of sequence in fragments shorter than    8113 bp
  85% of sequence in fragments shorter than    9594 bp
  90% of sequence in fragments shorter than   11766 bp
  95% of sequence in fragments shorter than   16043 bp

# HhaI.SacI (GCGC+GAGCTC) double digest, 2359 thousand fragments (1756502+603323 sites), 1263 bp average fragment length
   5% of sequence in fragments shorter than     412 bp
  10% of sequence in fragments shorter than     695 bp
  15% of sequence in fragments shorter than     979 bp
  20% of sequence in fragments shorter than    1252 bp
  25% of sequence in fragments shorter than    1529 bp
  30% of sequence in fragments shorter than    1816 bp
  35% of sequence in fragments shorter than    2125 bp
  40% of sequence in fragments shorter than    2455 bp
  45% of sequence in fragments shorter than    2810 bp
  50% of sequence in fragments shorter than    3201 bp
  55% of sequence in fragments shorter than    3629 bp
  60% of sequence in fragments shorter than    4104 bp
  65% of sequence in fragments shorter than    4616 bp
  70% of sequence in fragments shorter than    5222 bp
  75% of sequence in fragments shorter than    5941 bp
  80% of sequence in fragments shorter than    6814 bp
  85% of sequence in fragments shorter than    7934 bp
  90% of sequence in fragments shorter than    9506 bp
  95% of sequence in fragments shorter than   12298 bp

# BceAI.PsiI (ACGGC+TTATAA) double digest, 2352 thousand fragments (705178+1647912 sites), 1266 bp average fragment length
   5% of sequence in fragments shorter than     439 bp
  10% of sequence in fragments shorter than     673 bp
  15% of sequence in fragments shorter than     879 bp
  20% of sequence in fragments shorter than    1073 bp
  25% of sequence in fragments shorter than    1264 bp
  30% of sequence in fragments shorter than    1454 bp
  35% of sequence in fragments shorter than    1649 bp
  40% of sequence in fragments shorter than    1851 bp
  45% of sequence in fragments shorter than    2055 bp
  50% of sequence in fragments shorter than    2283 bp
  55% of sequence in fragments shorter than    2524 bp
  60% of sequence in fragments shorter than    2790 bp
  65% of sequence in fragments shorter than    3079 bp
  70% of sequence in fragments shorter than    3416 bp
  75% of sequence in fragments shorter than    3807 bp
  80% of sequence in fragments shorter than    4281 bp
  85% of sequence in fragments shorter than    4906 bp
  90% of sequence in fragments shorter than    5798 bp
  95% of sequence in fragments shorter than    7488 bp

# AvrII.HhaI (CCTAGG+GCGC) double digest, 2350 thousand fragments (594956+1756502 sites), 1267 bp average fragment length
   5% of sequence in fragments shorter than     424 bp
  10% of sequence in fragments shorter than     695 bp
  15% of sequence in fragments shorter than     982 bp
  20% of sequence in fragments shorter than    1249 bp
  25% of sequence in fragments shorter than    1525 bp
  30% of sequence in fragments shorter than    1801 bp
  35% of sequence in fragments shorter than    2097 bp
  40% of sequence in fragments shorter than    2410 bp
  45% of sequence in fragments shorter than    2739 bp
  50% of sequence in fragments shorter than    3101 bp
  55% of sequence in fragments shorter than    3480 bp
  60% of sequence in fragments shorter than    3912 bp
  65% of sequence in fragments shorter than    4380 bp
  70% of sequence in fragments shorter than    4935 bp
  75% of sequence in fragments shorter than    5586 bp
  80% of sequence in fragments shorter than    6378 bp
  85% of sequence in fragments shorter than    7407 bp
  90% of sequence in fragments shorter than    8829 bp
  95% of sequence in fragments shorter than   11332 bp

# AflII.BfiI (CTTAAG+ACTGGG) double digest, 2349 thousand fragments (643284+1706594 sites), 1268 bp average fragment length
   5% of sequence in fragments shorter than     434 bp
  10% of sequence in fragments shorter than     668 bp
  15% of sequence in fragments shorter than     869 bp
  20% of sequence in fragments shorter than    1052 bp
  25% of sequence in fragments shorter than    1236 bp
  30% of sequence in fragments shorter than    1396 bp
  35% of sequence in fragments shorter than    1580 bp
  40% of sequence in fragments shorter than    1768 bp
  45% of sequence in fragments shorter than    1970 bp
  50% of sequence in fragments shorter than    2176 bp
  55% of sequence in fragments shorter than    2399 bp
  60% of sequence in fragments shorter than    2651 bp
  65% of sequence in fragments shorter than    2929 bp
  70% of sequence in fragments shorter than    3248 bp
  75% of sequence in fragments shorter than    3613 bp
  80% of sequence in fragments shorter than    4060 bp
  85% of sequence in fragments shorter than    4638 bp
  90% of sequence in fragments shorter than    5468 bp
  95% of sequence in fragments shorter than    7070 bp

# HpaII.SalI (CCGG+GTCGAC) double digest, 2348 thousand fragments (2317722+31396 sites), 1268 bp average fragment length
   5% of sequence in fragments shorter than     393 bp
  10% of sequence in fragments shorter than     716 bp
  15% of sequence in fragments shorter than    1024 bp
  20% of sequence in fragments shorter than    1336 bp
  25% of sequence in fragments shorter than    1657 bp
  30% of sequence in fragments shorter than    1997 bp
  35% of sequence in fragments shorter than    2360 bp
  40% of sequence in fragments shorter than    2760 bp
  45% of sequence in fragments shorter than    3193 bp
  50% of sequence in fragments shorter than    3668 bp
  55% of sequence in fragments shorter than    4196 bp
  60% of sequence in fragments shorter than    4794 bp
  65% of sequence in fragments shorter than    5472 bp
  70% of sequence in fragments shorter than    6251 bp
  75% of sequence in fragments shorter than    7196 bp
  80% of sequence in fragments shorter than    8366 bp
  85% of sequence in fragments shorter than    9933 bp
  90% of sequence in fragments shorter than   12242 bp
  95% of sequence in fragments shorter than   16756 bp

# AflIII.HindIII (ACRYGT+AAGCTT) double digest, 2345 thousand fragments (1485394+860365 sites), 1270 bp average fragment length
   5% of sequence in fragments shorter than     444 bp
  10% of sequence in fragments shorter than     689 bp
  15% of sequence in fragments shorter than     899 bp
  20% of sequence in fragments shorter than    1097 bp
  25% of sequence in fragments shorter than    1285 bp
  30% of sequence in fragments shorter than    1474 bp
  35% of sequence in fragments shorter than    1668 bp
  40% of sequence in fragments shorter than    1867 bp
  45% of sequence in fragments shorter than    2040 bp
  50% of sequence in fragments shorter than    2250 bp
  55% of sequence in fragments shorter than    2479 bp
  60% of sequence in fragments shorter than    2735 bp
  65% of sequence in fragments shorter than    3014 bp
  70% of sequence in fragments shorter than    3332 bp
  75% of sequence in fragments shorter than    3706 bp
  80% of sequence in fragments shorter than    4155 bp
  85% of sequence in fragments shorter than    4740 bp
  90% of sequence in fragments shorter than    5582 bp
  95% of sequence in fragments shorter than    7120 bp

# BciVI.PsiI (GTATCC+TTATAA) double digest, 2343 thousand fragments (696093+1647912 sites), 1271 bp average fragment length
   5% of sequence in fragments shorter than     432 bp
  10% of sequence in fragments shorter than     665 bp
  15% of sequence in fragments shorter than     875 bp
  20% of sequence in fragments shorter than    1071 bp
  25% of sequence in fragments shorter than    1263 bp
  30% of sequence in fragments shorter than    1451 bp
  35% of sequence in fragments shorter than    1648 bp
  40% of sequence in fragments shorter than    1858 bp
  45% of sequence in fragments shorter than    2080 bp
  50% of sequence in fragments shorter than    2320 bp
  55% of sequence in fragments shorter than    2587 bp
  60% of sequence in fragments shorter than    2883 bp
  65% of sequence in fragments shorter than    3213 bp
  70% of sequence in fragments shorter than    3605 bp
  75% of sequence in fragments shorter than    4080 bp
  80% of sequence in fragments shorter than    4676 bp
  85% of sequence in fragments shorter than    5491 bp
  90% of sequence in fragments shorter than    6789 bp
  95% of sequence in fragments shorter than    9562 bp

# FauI.NdeI (CCCGC+CATATG) double digest, 2342 thousand fragments (1439772+903360 sites), 1272 bp average fragment length
   5% of sequence in fragments shorter than     444 bp
  10% of sequence in fragments shorter than     731 bp
  15% of sequence in fragments shorter than     973 bp
  20% of sequence in fragments shorter than    1193 bp
  25% of sequence in fragments shorter than    1423 bp
  30% of sequence in fragments shorter than    1658 bp
  35% of sequence in fragments shorter than    1898 bp
  40% of sequence in fragments shorter than    2143 bp
  45% of sequence in fragments shorter than    2407 bp
  50% of sequence in fragments shorter than    2685 bp
  55% of sequence in fragments shorter than    2984 bp
  60% of sequence in fragments shorter than    3309 bp
  65% of sequence in fragments shorter than    3673 bp
  70% of sequence in fragments shorter than    4083 bp
  75% of sequence in fragments shorter than    4524 bp
  80% of sequence in fragments shorter than    5091 bp
  85% of sequence in fragments shorter than    5832 bp
  90% of sequence in fragments shorter than    6892 bp
  95% of sequence in fragments shorter than    8851 bp

# BclI.EsaBC3I (TGATCA+TCGA) double digest, 2341 thousand fragments (738785+1603346 sites), 1272 bp average fragment length
   5% of sequence in fragments shorter than     441 bp
  10% of sequence in fragments shorter than     674 bp
  15% of sequence in fragments shorter than     871 bp
  20% of sequence in fragments shorter than    1060 bp
  25% of sequence in fragments shorter than    1243 bp
  30% of sequence in fragments shorter than    1420 bp
  35% of sequence in fragments shorter than    1609 bp
  40% of sequence in fragments shorter than    1798 bp
  45% of sequence in fragments shorter than    2003 bp
  50% of sequence in fragments shorter than    2218 bp
  55% of sequence in fragments shorter than    2451 bp
  60% of sequence in fragments shorter than    2702 bp
  65% of sequence in fragments shorter than    2976 bp
  70% of sequence in fragments shorter than    3288 bp
  75% of sequence in fragments shorter than    3655 bp
  80% of sequence in fragments shorter than    4097 bp
  85% of sequence in fragments shorter than    4654 bp
  90% of sequence in fragments shorter than    5448 bp
  95% of sequence in fragments shorter than    6808 bp

# BspMI.NdeI (ACCTGC+CATATG) double digest, 2341 thousand fragments (1438129+903360 sites), 1272 bp average fragment length
   5% of sequence in fragments shorter than     439 bp
  10% of sequence in fragments shorter than     685 bp
  15% of sequence in fragments shorter than     874 bp
  20% of sequence in fragments shorter than    1065 bp
  25% of sequence in fragments shorter than    1239 bp
  30% of sequence in fragments shorter than    1426 bp
  35% of sequence in fragments shorter than    1617 bp
  40% of sequence in fragments shorter than    1806 bp
  45% of sequence in fragments shorter than    1996 bp
  50% of sequence in fragments shorter than    2210 bp
  55% of sequence in fragments shorter than    2442 bp
  60% of sequence in fragments shorter than    2695 bp
  65% of sequence in fragments shorter than    2970 bp
  70% of sequence in fragments shorter than    3282 bp
  75% of sequence in fragments shorter than    3644 bp
  80% of sequence in fragments shorter than    4079 bp
  85% of sequence in fragments shorter than    4633 bp
  90% of sequence in fragments shorter than    5417 bp
  95% of sequence in fragments shorter than    6772 bp

# HpaII.MluI (CCGG+ACGCGT) double digest, 2338 thousand fragments (2317722+21415 sites), 1274 bp average fragment length
   5% of sequence in fragments shorter than     394 bp
  10% of sequence in fragments shorter than     720 bp
  15% of sequence in fragments shorter than    1031 bp
  20% of sequence in fragments shorter than    1346 bp
  25% of sequence in fragments shorter than    1672 bp
  30% of sequence in fragments shorter than    2016 bp
  35% of sequence in fragments shorter than    2384 bp
  40% of sequence in fragments shorter than    2792 bp
  45% of sequence in fragments shorter than    3232 bp
  50% of sequence in fragments shorter than    3715 bp
  55% of sequence in fragments shorter than    4250 bp
  60% of sequence in fragments shorter than    4861 bp
  65% of sequence in fragments shorter than    5554 bp
  70% of sequence in fragments shorter than    6341 bp
  75% of sequence in fragments shorter than    7306 bp
  80% of sequence in fragments shorter than    8508 bp
  85% of sequence in fragments shorter than   10093 bp
  90% of sequence in fragments shorter than   12430 bp
  95% of sequence in fragments shorter than   17093 bp

# EsaBC3I.FnuDII (TCGA+CGCG) double digest, 2336 thousand fragments (1603346+733940 sites), 1275 bp average fragment length
   5% of sequence in fragments shorter than     445 bp
  10% of sequence in fragments shorter than     737 bp
  15% of sequence in fragments shorter than     994 bp
  20% of sequence in fragments shorter than    1239 bp
  25% of sequence in fragments shorter than    1476 bp
  30% of sequence in fragments shorter than    1728 bp
  35% of sequence in fragments shorter than    1987 bp
  40% of sequence in fragments shorter than    2260 bp
  45% of sequence in fragments shorter than    2548 bp
  50% of sequence in fragments shorter than    2855 bp
  55% of sequence in fragments shorter than    3184 bp
  60% of sequence in fragments shorter than    3552 bp
  65% of sequence in fragments shorter than    3963 bp
  70% of sequence in fragments shorter than    4435 bp
  75% of sequence in fragments shorter than    4985 bp
  80% of sequence in fragments shorter than    5662 bp
  85% of sequence in fragments shorter than    6530 bp
  90% of sequence in fragments shorter than    7738 bp
  95% of sequence in fragments shorter than    9822 bp

# BfiI.HaeII (ACTGGG+RGCGCY) double digest, 2336 thousand fragments (1706594+630101 sites), 1275 bp average fragment length
   5% of sequence in fragments shorter than     408 bp
  10% of sequence in fragments shorter than     666 bp
  15% of sequence in fragments shorter than     870 bp
  20% of sequence in fragments shorter than    1078 bp
  25% of sequence in fragments shorter than    1293 bp
  30% of sequence in fragments shorter than    1508 bp
  35% of sequence in fragments shorter than    1728 bp
  40% of sequence in fragments shorter than    1953 bp
  45% of sequence in fragments shorter than    2188 bp
  50% of sequence in fragments shorter than    2443 bp
  55% of sequence in fragments shorter than    2727 bp
  60% of sequence in fragments shorter than    3007 bp
  65% of sequence in fragments shorter than    3347 bp
  70% of sequence in fragments shorter than    3735 bp
  75% of sequence in fragments shorter than    4191 bp
  80% of sequence in fragments shorter than    4737 bp
  85% of sequence in fragments shorter than    5421 bp
  90% of sequence in fragments shorter than    6401 bp
  95% of sequence in fragments shorter than    8111 bp

# AflIII.XbaI (ACRYGT+TCTAGA) double digest, 2335 thousand fragments (1485394+850998 sites), 1275 bp average fragment length
   5% of sequence in fragments shorter than     446 bp
  10% of sequence in fragments shorter than     693 bp
  15% of sequence in fragments shorter than     899 bp
  20% of sequence in fragments shorter than    1100 bp
  25% of sequence in fragments shorter than    1299 bp
  30% of sequence in fragments shorter than    1482 bp
  35% of sequence in fragments shorter than    1683 bp
  40% of sequence in fragments shorter than    1884 bp
  45% of sequence in fragments shorter than    2091 bp
  50% of sequence in fragments shorter than    2310 bp
  55% of sequence in fragments shorter than    2553 bp
  60% of sequence in fragments shorter than    2818 bp
  65% of sequence in fragments shorter than    3108 bp
  70% of sequence in fragments shorter than    3441 bp
  75% of sequence in fragments shorter than    3817 bp
  80% of sequence in fragments shorter than    4276 bp
  85% of sequence in fragments shorter than    4856 bp
  90% of sequence in fragments shorter than    5678 bp
  95% of sequence in fragments shorter than    7123 bp

# HpaII.SgrAI (CCGG+CRCCGGYG) double digest, 2332 thousand fragments (2317722+15270 sites), 1277 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     727 bp
  15% of sequence in fragments shorter than    1041 bp
  20% of sequence in fragments shorter than    1359 bp
  25% of sequence in fragments shorter than    1684 bp
  30% of sequence in fragments shorter than    2035 bp
  35% of sequence in fragments shorter than    2406 bp
  40% of sequence in fragments shorter than    2818 bp
  45% of sequence in fragments shorter than    3263 bp
  50% of sequence in fragments shorter than    3751 bp
  55% of sequence in fragments shorter than    4291 bp
  60% of sequence in fragments shorter than    4906 bp
  65% of sequence in fragments shorter than    5603 bp
  70% of sequence in fragments shorter than    6398 bp
  75% of sequence in fragments shorter than    7370 bp
  80% of sequence in fragments shorter than    8590 bp
  85% of sequence in fragments shorter than   10188 bp
  90% of sequence in fragments shorter than   12542 bp
  95% of sequence in fragments shorter than   17302 bp

# AflIII.EcoRI (ACRYGT+GAATTC) double digest, 2332 thousand fragments (1485394+847401 sites), 1277 bp average fragment length
   5% of sequence in fragments shorter than     436 bp
  10% of sequence in fragments shorter than     689 bp
  15% of sequence in fragments shorter than     896 bp
  20% of sequence in fragments shorter than    1099 bp
  25% of sequence in fragments shorter than    1297 bp
  30% of sequence in fragments shorter than    1486 bp
  35% of sequence in fragments shorter than    1684 bp
  40% of sequence in fragments shorter than    1886 bp
  45% of sequence in fragments shorter than    2090 bp
  50% of sequence in fragments shorter than    2301 bp
  55% of sequence in fragments shorter than    2536 bp
  60% of sequence in fragments shorter than    2791 bp
  65% of sequence in fragments shorter than    3081 bp
  70% of sequence in fragments shorter than    3399 bp
  75% of sequence in fragments shorter than    3774 bp
  80% of sequence in fragments shorter than    4232 bp
  85% of sequence in fragments shorter than    4808 bp
  90% of sequence in fragments shorter than    5621 bp
  95% of sequence in fragments shorter than    7021 bp

# HpaII.NruI (CCGG+TCGCGA) double digest, 2332 thousand fragments (2317722+14872 sites), 1277 bp average fragment length
   5% of sequence in fragments shorter than     396 bp
  10% of sequence in fragments shorter than     723 bp
  15% of sequence in fragments shorter than    1036 bp
  20% of sequence in fragments shorter than    1352 bp
  25% of sequence in fragments shorter than    1678 bp
  30% of sequence in fragments shorter than    2024 bp
  35% of sequence in fragments shorter than    2394 bp
  40% of sequence in fragments shorter than    2802 bp
  45% of sequence in fragments shorter than    3245 bp
  50% of sequence in fragments shorter than    3729 bp
  55% of sequence in fragments shorter than    4266 bp
  60% of sequence in fragments shorter than    4879 bp
  65% of sequence in fragments shorter than    5571 bp
  70% of sequence in fragments shorter than    6361 bp
  75% of sequence in fragments shorter than    7329 bp
  80% of sequence in fragments shorter than    8533 bp
  85% of sequence in fragments shorter than   10125 bp
  90% of sequence in fragments shorter than   12466 bp
  95% of sequence in fragments shorter than   17146 bp

# AccI.BscGI (GTMKAC+CCCGT) double digest, 2331 thousand fragments (1101063+1231070 sites), 1278 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     654 bp
  15% of sequence in fragments shorter than     869 bp
  20% of sequence in fragments shorter than    1078 bp
  25% of sequence in fragments shorter than    1283 bp
  30% of sequence in fragments shorter than    1486 bp
  35% of sequence in fragments shorter than    1695 bp
  40% of sequence in fragments shorter than    1909 bp
  45% of sequence in fragments shorter than    2134 bp
  50% of sequence in fragments shorter than    2371 bp
  55% of sequence in fragments shorter than    2627 bp
  60% of sequence in fragments shorter than    2904 bp
  65% of sequence in fragments shorter than    3211 bp
  70% of sequence in fragments shorter than    3554 bp
  75% of sequence in fragments shorter than    3952 bp
  80% of sequence in fragments shorter than    4434 bp
  85% of sequence in fragments shorter than    5004 bp
  90% of sequence in fragments shorter than    5802 bp
  95% of sequence in fragments shorter than    7163 bp

# HpaII.PvuI (CCGG+CGATCG) double digest, 2331 thousand fragments (2317722+14186 sites), 1278 bp average fragment length
   5% of sequence in fragments shorter than     396 bp
  10% of sequence in fragments shorter than     723 bp
  15% of sequence in fragments shorter than    1035 bp
  20% of sequence in fragments shorter than    1350 bp
  25% of sequence in fragments shorter than    1676 bp
  30% of sequence in fragments shorter than    2017 bp
  35% of sequence in fragments shorter than    2387 bp
  40% of sequence in fragments shorter than    2796 bp
  45% of sequence in fragments shorter than    3236 bp
  50% of sequence in fragments shorter than    3720 bp
  55% of sequence in fragments shorter than    4256 bp
  60% of sequence in fragments shorter than    4865 bp
  65% of sequence in fragments shorter than    5557 bp
  70% of sequence in fragments shorter than    6345 bp
  75% of sequence in fragments shorter than    7312 bp
  80% of sequence in fragments shorter than    8512 bp
  85% of sequence in fragments shorter than   10095 bp
  90% of sequence in fragments shorter than   12416 bp
  95% of sequence in fragments shorter than   16999 bp

# FseI.HpaII (GGCCGGCC+CCGG) double digest, 2330 thousand fragments (13662+2317722 sites), 1278 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     727 bp
  15% of sequence in fragments shorter than    1041 bp
  20% of sequence in fragments shorter than    1359 bp
  25% of sequence in fragments shorter than    1684 bp
  30% of sequence in fragments shorter than    2035 bp
  35% of sequence in fragments shorter than    2406 bp
  40% of sequence in fragments shorter than    2818 bp
  45% of sequence in fragments shorter than    3263 bp
  50% of sequence in fragments shorter than    3751 bp
  55% of sequence in fragments shorter than    4291 bp
  60% of sequence in fragments shorter than    4906 bp
  65% of sequence in fragments shorter than    5603 bp
  70% of sequence in fragments shorter than    6398 bp
  75% of sequence in fragments shorter than    7370 bp
  80% of sequence in fragments shorter than    8590 bp
  85% of sequence in fragments shorter than   10188 bp
  90% of sequence in fragments shorter than   12542 bp
  95% of sequence in fragments shorter than   17302 bp

# HgaI.HhaI (GACGC+GCGC) double digest, 2327 thousand fragments (571889+1756502 sites), 1280 bp average fragment length
   5% of sequence in fragments shorter than     412 bp
  10% of sequence in fragments shorter than     734 bp
  15% of sequence in fragments shorter than    1046 bp
  20% of sequence in fragments shorter than    1375 bp
  25% of sequence in fragments shorter than    1711 bp
  30% of sequence in fragments shorter than    2054 bp
  35% of sequence in fragments shorter than    2428 bp
  40% of sequence in fragments shorter than    2827 bp
  45% of sequence in fragments shorter than    3254 bp
  50% of sequence in fragments shorter than    3734 bp
  55% of sequence in fragments shorter than    4264 bp
  60% of sequence in fragments shorter than    4856 bp
  65% of sequence in fragments shorter than    5523 bp
  70% of sequence in fragments shorter than    6296 bp
  75% of sequence in fragments shorter than    7203 bp
  80% of sequence in fragments shorter than    8321 bp
  85% of sequence in fragments shorter than    9777 bp
  90% of sequence in fragments shorter than   11832 bp
  95% of sequence in fragments shorter than   15372 bp

# EciI.HhaI (GGCGGA+GCGC) double digest, 2327 thousand fragments (571273+1756502 sites), 1280 bp average fragment length
   5% of sequence in fragments shorter than     415 bp
  10% of sequence in fragments shorter than     763 bp
  15% of sequence in fragments shorter than    1100 bp
  20% of sequence in fragments shorter than    1448 bp
  25% of sequence in fragments shorter than    1805 bp
  30% of sequence in fragments shorter than    2192 bp
  35% of sequence in fragments shorter than    2612 bp
  40% of sequence in fragments shorter than    3065 bp
  45% of sequence in fragments shorter than    3554 bp
  50% of sequence in fragments shorter than    4107 bp
  55% of sequence in fragments shorter than    4708 bp
  60% of sequence in fragments shorter than    5385 bp
  65% of sequence in fragments shorter than    6149 bp
  70% of sequence in fragments shorter than    7039 bp
  75% of sequence in fragments shorter than    8093 bp
  80% of sequence in fragments shorter than    9399 bp
  85% of sequence in fragments shorter than   11083 bp
  90% of sequence in fragments shorter than   13532 bp
  95% of sequence in fragments shorter than   17724 bp

# HpaII.NotI (CCGG+GCGGCCGC) double digest, 2327 thousand fragments (2317722+9795 sites), 1280 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     726 bp
  15% of sequence in fragments shorter than    1040 bp
  20% of sequence in fragments shorter than    1358 bp
  25% of sequence in fragments shorter than    1683 bp
  30% of sequence in fragments shorter than    2034 bp
  35% of sequence in fragments shorter than    2405 bp
  40% of sequence in fragments shorter than    2816 bp
  45% of sequence in fragments shorter than    3262 bp
  50% of sequence in fragments shorter than    3749 bp
  55% of sequence in fragments shorter than    4289 bp
  60% of sequence in fragments shorter than    4905 bp
  65% of sequence in fragments shorter than    5601 bp
  70% of sequence in fragments shorter than    6396 bp
  75% of sequence in fragments shorter than    7367 bp
  80% of sequence in fragments shorter than    8588 bp
  85% of sequence in fragments shorter than   10185 bp
  90% of sequence in fragments shorter than   12538 bp
  95% of sequence in fragments shorter than   17302 bp

# HpaII.RsrII (CCGG+CGGWCCG) double digest, 2327 thousand fragments (2317722+9764 sites), 1280 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     726 bp
  15% of sequence in fragments shorter than    1040 bp
  20% of sequence in fragments shorter than    1357 bp
  25% of sequence in fragments shorter than    1682 bp
  30% of sequence in fragments shorter than    2032 bp
  35% of sequence in fragments shorter than    2404 bp
  40% of sequence in fragments shorter than    2815 bp
  45% of sequence in fragments shorter than    3260 bp
  50% of sequence in fragments shorter than    3747 bp
  55% of sequence in fragments shorter than    4286 bp
  60% of sequence in fragments shorter than    4901 bp
  65% of sequence in fragments shorter than    5597 bp
  70% of sequence in fragments shorter than    6391 bp
  75% of sequence in fragments shorter than    7361 bp
  80% of sequence in fragments shorter than    8579 bp
  85% of sequence in fragments shorter than   10175 bp
  90% of sequence in fragments shorter than   12527 bp
  95% of sequence in fragments shorter than   17283 bp

# AvaI.PstI (CYCGRG+CTGCAG) double digest, 2326 thousand fragments (1005714+1321469 sites), 1280 bp average fragment length
   5% of sequence in fragments shorter than     414 bp
  10% of sequence in fragments shorter than     664 bp
  15% of sequence in fragments shorter than     891 bp
  20% of sequence in fragments shorter than    1118 bp
  25% of sequence in fragments shorter than    1351 bp
  30% of sequence in fragments shorter than    1588 bp
  35% of sequence in fragments shorter than    1844 bp
  40% of sequence in fragments shorter than    2108 bp
  45% of sequence in fragments shorter than    2401 bp
  50% of sequence in fragments shorter than    2722 bp
  55% of sequence in fragments shorter than    3069 bp
  60% of sequence in fragments shorter than    3463 bp
  65% of sequence in fragments shorter than    3916 bp
  70% of sequence in fragments shorter than    4441 bp
  75% of sequence in fragments shorter than    5048 bp
  80% of sequence in fragments shorter than    5784 bp
  85% of sequence in fragments shorter than    6738 bp
  90% of sequence in fragments shorter than    8139 bp
  95% of sequence in fragments shorter than   10725 bp

# BglII.HgiCI (AGATCT+GGYRCC) double digest, 2325 thousand fragments (774732+1550877 sites), 1281 bp average fragment length
   5% of sequence in fragments shorter than     432 bp
  10% of sequence in fragments shorter than     662 bp
  15% of sequence in fragments shorter than     865 bp
  20% of sequence in fragments shorter than    1059 bp
  25% of sequence in fragments shorter than    1238 bp
  30% of sequence in fragments shorter than    1425 bp
  35% of sequence in fragments shorter than    1619 bp
  40% of sequence in fragments shorter than    1822 bp
  45% of sequence in fragments shorter than    2039 bp
  50% of sequence in fragments shorter than    2270 bp
  55% of sequence in fragments shorter than    2520 bp
  60% of sequence in fragments shorter than    2793 bp
  65% of sequence in fragments shorter than    3098 bp
  70% of sequence in fragments shorter than    3445 bp
  75% of sequence in fragments shorter than    3857 bp
  80% of sequence in fragments shorter than    4365 bp
  85% of sequence in fragments shorter than    5034 bp
  90% of sequence in fragments shorter than    6026 bp
  95% of sequence in fragments shorter than    8054 bp

# BglII.BsmI (AGATCT+GAATGC) double digest, 2323 thousand fragments (774732+1549350 sites), 1282 bp average fragment length
   5% of sequence in fragments shorter than     422 bp
  10% of sequence in fragments shorter than     705 bp
  15% of sequence in fragments shorter than     944 bp
  20% of sequence in fragments shorter than    1152 bp
  25% of sequence in fragments shorter than    1365 bp
  30% of sequence in fragments shorter than    1578 bp
  35% of sequence in fragments shorter than    1791 bp
  40% of sequence in fragments shorter than    2012 bp
  45% of sequence in fragments shorter than    2239 bp
  50% of sequence in fragments shorter than    2479 bp
  55% of sequence in fragments shorter than    2736 bp
  60% of sequence in fragments shorter than    3015 bp
  65% of sequence in fragments shorter than    3323 bp
  70% of sequence in fragments shorter than    3671 bp
  75% of sequence in fragments shorter than    4073 bp
  80% of sequence in fragments shorter than    4555 bp
  85% of sequence in fragments shorter than    5169 bp
  90% of sequence in fragments shorter than    6009 bp
  95% of sequence in fragments shorter than    7447 bp

# AscI.HpaII (GGCGCGCC+CCGG) double digest, 2322 thousand fragments (4751+2317722 sites), 1283 bp average fragment length
   5% of sequence in fragments shorter than     398 bp
  10% of sequence in fragments shorter than     727 bp
  15% of sequence in fragments shorter than    1040 bp
  20% of sequence in fragments shorter than    1358 bp
  25% of sequence in fragments shorter than    1683 bp
  30% of sequence in fragments shorter than    2034 bp
  35% of sequence in fragments shorter than    2405 bp
  40% of sequence in fragments shorter than    2817 bp
  45% of sequence in fragments shorter than    3262 bp
  50% of sequence in fragments shorter than    3750 bp
  55% of sequence in fragments shorter than    4290 bp
  60% of sequence in fragments shorter than    4905 bp
  65% of sequence in fragments shorter than    5601 bp
  70% of sequence in fragments shorter than    6397 bp
  75% of sequence in fragments shorter than    7369 bp
  80% of sequence in fragments shorter than    8588 bp
  85% of sequence in fragments shorter than   10186 bp
  90% of sequence in fragments shorter than   12539 bp
  95% of sequence in fragments shorter than   17300 bp

# HhaI.MfeI (GCGC+CAATTG) double digest, 2320 thousand fragments (1756502+564303 sites), 1284 bp average fragment length
   5% of sequence in fragments shorter than     442 bp
  10% of sequence in fragments shorter than     734 bp
  15% of sequence in fragments shorter than    1014 bp
  20% of sequence in fragments shorter than    1275 bp
  25% of sequence in fragments shorter than    1539 bp
  30% of sequence in fragments shorter than    1793 bp
  35% of sequence in fragments shorter than    2065 bp
  40% of sequence in fragments shorter than    2349 bp
  45% of sequence in fragments shorter than    2641 bp
  50% of sequence in fragments shorter than    2959 bp
  55% of sequence in fragments shorter than    3303 bp
  60% of sequence in fragments shorter than    3669 bp
  65% of sequence in fragments shorter than    4074 bp
  70% of sequence in fragments shorter than    4539 bp
  75% of sequence in fragments shorter than    5088 bp
  80% of sequence in fragments shorter than    5749 bp
  85% of sequence in fragments shorter than    6591 bp
  90% of sequence in fragments shorter than    7749 bp
  95% of sequence in fragments shorter than    9714 bp

# BsaAI.EsaBC3I (YACGTR+TCGA) double digest, 2316 thousand fragments (713801+1603346 sites), 1286 bp average fragment length
   5% of sequence in fragments shorter than     444 bp
  10% of sequence in fragments shorter than     681 bp
  15% of sequence in fragments shorter than     880 bp
  20% of sequence in fragments shorter than    1069 bp
  25% of sequence in fragments shorter than    1258 bp
  30% of sequence in fragments shorter than    1447 bp
  35% of sequence in fragments shorter than    1647 bp
  40% of sequence in fragments shorter than    1851 bp
  45% of sequence in fragments shorter than    2066 bp
  50% of sequence in fragments shorter than    2296 bp
  55% of sequence in fragments shorter than    2538 bp
  60% of sequence in fragments shorter than    2806 bp
  65% of sequence in fragments shorter than    3107 bp
  70% of sequence in fragments shorter than    3447 bp
  75% of sequence in fragments shorter than    3839 bp
  80% of sequence in fragments shorter than    4321 bp
  85% of sequence in fragments shorter than    4932 bp
  90% of sequence in fragments shorter than    5787 bp
  95% of sequence in fragments shorter than    7280 bp

# BscGI.PpuMI (CCCGT+RGGWCCY) double digest, 2315 thousand fragments (1231070+1085138 sites), 1286 bp average fragment length
   5% of sequence in fragments shorter than     362 bp
  10% of sequence in fragments shorter than     633 bp
  15% of sequence in fragments shorter than     871 bp
  20% of sequence in fragments shorter than    1115 bp
  25% of sequence in fragments shorter than    1366 bp
  30% of sequence in fragments shorter than    1619 bp
  35% of sequence in fragments shorter than    1885 bp
  40% of sequence in fragments shorter than    2164 bp
  45% of sequence in fragments shorter than    2460 bp
  50% of sequence in fragments shorter than    2781 bp
  55% of sequence in fragments shorter than    3121 bp
  60% of sequence in fragments shorter than    3493 bp
  65% of sequence in fragments shorter than    3904 bp
  70% of sequence in fragments shorter than    4391 bp
  75% of sequence in fragments shorter than    4972 bp
  80% of sequence in fragments shorter than    5684 bp
  85% of sequence in fragments shorter than    6595 bp
  90% of sequence in fragments shorter than    7871 bp
  95% of sequence in fragments shorter than   10066 bp

# BscGI.PvuII (CCCGT+CAGCTG) double digest, 2314 thousand fragments (1231070+1084260 sites), 1287 bp average fragment length
   5% of sequence in fragments shorter than     371 bp
  10% of sequence in fragments shorter than     632 bp
  15% of sequence in fragments shorter than     862 bp
  20% of sequence in fragments shorter than    1089 bp
  25% of sequence in fragments shorter than    1325 bp
  30% of sequence in fragments shorter than    1565 bp
  35% of sequence in fragments shorter than    1817 bp
  40% of sequence in fragments shorter than    2082 bp
  45% of sequence in fragments shorter than    2367 bp
  50% of sequence in fragments shorter than    2670 bp
  55% of sequence in fragments shorter than    2999 bp
  60% of sequence in fragments shorter than    3373 bp
  65% of sequence in fragments shorter than    3787 bp
  70% of sequence in fragments shorter than    4267 bp
  75% of sequence in fragments shorter than    4833 bp
  80% of sequence in fragments shorter than    5519 bp
  85% of sequence in fragments shorter than    6387 bp
  90% of sequence in fragments shorter than    7586 bp
  95% of sequence in fragments shorter than    9707 bp

# HgiCI.NcoI (GGYRCC+CCATGG) double digest, 2310 thousand fragments (1550877+759594 sites), 1290 bp average fragment length
   5% of sequence in fragments shorter than     431 bp
  10% of sequence in fragments shorter than     673 bp
  15% of sequence in fragments shorter than     890 bp
  20% of sequence in fragments shorter than    1084 bp
  25% of sequence in fragments shorter than    1275 bp
  30% of sequence in fragments shorter than    1481 bp
  35% of sequence in fragments shorter than    1692 bp
  40% of sequence in fragments shorter than    1923 bp
  45% of sequence in fragments shorter than    2169 bp
  50% of sequence in fragments shorter than    2424 bp
  55% of sequence in fragments shorter than    2709 bp
  60% of sequence in fragments shorter than    3010 bp
  65% of sequence in fragments shorter than    3360 bp
  70% of sequence in fragments shorter than    3764 bp
  75% of sequence in fragments shorter than    4237 bp
  80% of sequence in fragments shorter than    4826 bp
  85% of sequence in fragments shorter than    5604 bp
  90% of sequence in fragments shorter than    6771 bp
  95% of sequence in fragments shorter than    9069 bp

# BfiI.SacI (ACTGGG+GAGCTC) double digest, 2309 thousand fragments (1706594+603323 sites), 1290 bp average fragment length
   5% of sequence in fragments shorter than     431 bp
  10% of sequence in fragments shorter than     676 bp
  15% of sequence in fragments shorter than     886 bp
  20% of sequence in fragments shorter than    1075 bp
  25% of sequence in fragments shorter than    1267 bp
  30% of sequence in fragments shorter than    1466 bp
  35% of sequence in fragments shorter than    1672 bp
  40% of sequence in fragments shorter than    1885 bp
  45% of sequence in fragments shorter than    2106 bp
  50% of sequence in fragments shorter than    2348 bp
  55% of sequence in fragments shorter than    2611 bp
  60% of sequence in fragments shorter than    2870 bp
  65% of sequence in fragments shorter than    3192 bp
  70% of sequence in fragments shorter than    3553 bp
  75% of sequence in fragments shorter than    3974 bp
  80% of sequence in fragments shorter than    4491 bp
  85% of sequence in fragments shorter than    5166 bp
  90% of sequence in fragments shorter than    6133 bp
  95% of sequence in fragments shorter than    7987 bp

# BsmI.NcoI (GAATGC+CCATGG) double digest, 2308 thousand fragments (1549350+759594 sites), 1290 bp average fragment length
   5% of sequence in fragments shorter than     416 bp
  10% of sequence in fragments shorter than     719 bp
  15% of sequence in fragments shorter than     966 bp
  20% of sequence in fragments shorter than    1190 bp
  25% of sequence in fragments shorter than    1409 bp
  30% of sequence in fragments shorter than    1612 bp
  35% of sequence in fragments shorter than    1828 bp
  40% of sequence in fragments shorter than    2057 bp
  45% of sequence in fragments shorter than    2296 bp
  50% of sequence in fragments shorter than    2543 bp
  55% of sequence in fragments shorter than    2802 bp
  60% of sequence in fragments shorter than    3088 bp
  65% of sequence in fragments shorter than    3408 bp
  70% of sequence in fragments shorter than    3770 bp
  75% of sequence in fragments shorter than    4185 bp
  80% of sequence in fragments shorter than    4689 bp
  85% of sequence in fragments shorter than    5322 bp
  90% of sequence in fragments shorter than    6206 bp
  95% of sequence in fragments shorter than    7676 bp

# BceAI.EsaBC3I (ACGGC+TCGA) double digest, 2308 thousand fragments (705178+1603346 sites), 1291 bp average fragment length
   5% of sequence in fragments shorter than     433 bp
  10% of sequence in fragments shorter than     674 bp
  15% of sequence in fragments shorter than     887 bp
  20% of sequence in fragments shorter than    1092 bp
  25% of sequence in fragments shorter than    1301 bp
  30% of sequence in fragments shorter than    1504 bp
  35% of sequence in fragments shorter than    1724 bp
  40% of sequence in fragments shorter than    1951 bp
  45% of sequence in fragments shorter than    2193 bp
  50% of sequence in fragments shorter than    2451 bp
  55% of sequence in fragments shorter than    2733 bp
  60% of sequence in fragments shorter than    3041 bp
  65% of sequence in fragments shorter than    3384 bp
  70% of sequence in fragments shorter than    3780 bp
  75% of sequence in fragments shorter than    4245 bp
  80% of sequence in fragments shorter than    4809 bp
  85% of sequence in fragments shorter than    5526 bp
  90% of sequence in fragments shorter than    6531 bp
  95% of sequence in fragments shorter than    8276 bp

# HhaI.SphI (GCGC+GCATGC) double digest, 2306 thousand fragments (1756502+550952 sites), 1291 bp average fragment length
   5% of sequence in fragments shorter than     435 bp
  10% of sequence in fragments shorter than     739 bp
  15% of sequence in fragments shorter than    1034 bp
  20% of sequence in fragments shorter than    1320 bp
  25% of sequence in fragments shorter than    1612 bp
  30% of sequence in fragments shorter than    1906 bp
  35% of sequence in fragments shorter than    2224 bp
  40% of sequence in fragments shorter than    2563 bp
  45% of sequence in fragments shorter than    2927 bp
  50% of sequence in fragments shorter than    3326 bp
  55% of sequence in fragments shorter than    3759 bp
  60% of sequence in fragments shorter than    4232 bp
  65% of sequence in fragments shorter than    4780 bp
  70% of sequence in fragments shorter than    5398 bp
  75% of sequence in fragments shorter than    6119 bp
  80% of sequence in fragments shorter than    7002 bp
  85% of sequence in fragments shorter than    8143 bp
  90% of sequence in fragments shorter than    9753 bp
  95% of sequence in fragments shorter than   12515 bp

# AvrII.BfiI (CCTAGG+ACTGGG) double digest, 2301 thousand fragments (594956+1706594 sites), 1295 bp average fragment length
   5% of sequence in fragments shorter than     439 bp
  10% of sequence in fragments shorter than     655 bp
  15% of sequence in fragments shorter than     869 bp
  20% of sequence in fragments shorter than    1066 bp
  25% of sequence in fragments shorter than    1262 bp
  30% of sequence in fragments shorter than    1463 bp
  35% of sequence in fragments shorter than    1655 bp
  40% of sequence in fragments shorter than    1864 bp
  45% of sequence in fragments shorter than    2083 bp
  50% of sequence in fragments shorter than    2304 bp
  55% of sequence in fragments shorter than    2559 bp
  60% of sequence in fragments shorter than    2841 bp
  65% of sequence in fragments shorter than    3157 bp
  70% of sequence in fragments shorter than    3515 bp
  75% of sequence in fragments shorter than    3922 bp
  80% of sequence in fragments shorter than    4428 bp
  85% of sequence in fragments shorter than    5097 bp
  90% of sequence in fragments shorter than    6071 bp
  95% of sequence in fragments shorter than    7971 bp

# HhaI.ScaI (GCGC+AGTACT) double digest, 2299 thousand fragments (1756502+543793 sites), 1295 bp average fragment length
   5% of sequence in fragments shorter than     441 bp
  10% of sequence in fragments shorter than     734 bp
  15% of sequence in fragments shorter than    1019 bp
  20% of sequence in fragments shorter than    1282 bp
  25% of sequence in fragments shorter than    1550 bp
  30% of sequence in fragments shorter than    1814 bp
  35% of sequence in fragments shorter than    2097 bp
  40% of sequence in fragments shorter than    2397 bp
  45% of sequence in fragments shorter than    2710 bp
  50% of sequence in fragments shorter than    3049 bp
  55% of sequence in fragments shorter than    3420 bp
  60% of sequence in fragments shorter than    3826 bp
  65% of sequence in fragments shorter than    4268 bp
  70% of sequence in fragments shorter than    4793 bp
  75% of sequence in fragments shorter than    5396 bp
  80% of sequence in fragments shorter than    6121 bp
  85% of sequence in fragments shorter than    7054 bp
  90% of sequence in fragments shorter than    8353 bp
  95% of sequence in fragments shorter than   10596 bp

# FauI.HindIII (CCCGC+AAGCTT) double digest, 2299 thousand fragments (1439772+860365 sites), 1295 bp average fragment length
   5% of sequence in fragments shorter than     454 bp
  10% of sequence in fragments shorter than     739 bp
  15% of sequence in fragments shorter than     991 bp
  20% of sequence in fragments shorter than    1234 bp
  25% of sequence in fragments shorter than    1461 bp
  30% of sequence in fragments shorter than    1704 bp
  35% of sequence in fragments shorter than    1931 bp
  40% of sequence in fragments shorter than    2186 bp
  45% of sequence in fragments shorter than    2456 bp
  50% of sequence in fragments shorter than    2744 bp
  55% of sequence in fragments shorter than    3051 bp
  60% of sequence in fragments shorter than    3390 bp
  65% of sequence in fragments shorter than    3772 bp
  70% of sequence in fragments shorter than    4200 bp
  75% of sequence in fragments shorter than    4702 bp
  80% of sequence in fragments shorter than    5310 bp
  85% of sequence in fragments shorter than    6099 bp
  90% of sequence in fragments shorter than    7209 bp
  95% of sequence in fragments shorter than    9201 bp

# BspHI.PstI (TCATGA+CTGCAG) double digest, 2299 thousand fragments (978290+1321469 sites), 1296 bp average fragment length
   5% of sequence in fragments shorter than     453 bp
  10% of sequence in fragments shorter than     683 bp
  15% of sequence in fragments shorter than     883 bp
  20% of sequence in fragments shorter than    1079 bp
  25% of sequence in fragments shorter than    1269 bp
  30% of sequence in fragments shorter than    1460 bp
  35% of sequence in fragments shorter than    1658 bp
  40% of sequence in fragments shorter than    1862 bp
  45% of sequence in fragments shorter than    2065 bp
  50% of sequence in fragments shorter than    2286 bp
  55% of sequence in fragments shorter than    2523 bp
  60% of sequence in fragments shorter than    2777 bp
  65% of sequence in fragments shorter than    3044 bp
  70% of sequence in fragments shorter than    3365 bp
  75% of sequence in fragments shorter than    3736 bp
  80% of sequence in fragments shorter than    4190 bp
  85% of sequence in fragments shorter than    4774 bp
  90% of sequence in fragments shorter than    5605 bp
  95% of sequence in fragments shorter than    7119 bp

# BciVI.EsaBC3I (GTATCC+TCGA) double digest, 2298 thousand fragments (696093+1603346 sites), 1296 bp average fragment length
   5% of sequence in fragments shorter than     448 bp
  10% of sequence in fragments shorter than     682 bp
  15% of sequence in fragments shorter than     880 bp
  20% of sequence in fragments shorter than    1067 bp
  25% of sequence in fragments shorter than    1256 bp
  30% of sequence in fragments shorter than    1438 bp
  35% of sequence in fragments shorter than    1632 bp
  40% of sequence in fragments shorter than    1828 bp
  45% of sequence in fragments shorter than    2033 bp
  50% of sequence in fragments shorter than    2250 bp
  55% of sequence in fragments shorter than    2483 bp
  60% of sequence in fragments shorter than    2739 bp
  65% of sequence in fragments shorter than    3017 bp
  70% of sequence in fragments shorter than    3337 bp
  75% of sequence in fragments shorter than    3706 bp
  80% of sequence in fragments shorter than    4149 bp
  85% of sequence in fragments shorter than    4717 bp
  90% of sequence in fragments shorter than    5519 bp
  95% of sequence in fragments shorter than    6894 bp

# BspMI.HindIII (ACCTGC+AAGCTT) double digest, 2298 thousand fragments (1438129+860365 sites), 1296 bp average fragment length
   5% of sequence in fragments shorter than     446 bp
  10% of sequence in fragments shorter than     686 bp
  15% of sequence in fragments shorter than     889 bp
  20% of sequence in fragments shorter than    1062 bp
  25% of sequence in fragments shorter than    1254 bp
  30% of sequence in fragments shorter than    1443 bp
  35% of sequence in fragments shorter than    1641 bp
  40% of sequence in fragments shorter than    1846 bp
  45% of sequence in fragments shorter than    2050 bp
  50% of sequence in fragments shorter than    2273 bp
  55% of sequence in fragments shorter than    2484 bp
  60% of sequence in fragments shorter than    2743 bp
  65% of sequence in fragments shorter than    3027 bp
  70% of sequence in fragments shorter than    3348 bp
  75% of sequence in fragments shorter than    3726 bp
  80% of sequence in fragments shorter than    4179 bp
  85% of sequence in fragments shorter than    4747 bp
  90% of sequence in fragments shorter than    5544 bp
  95% of sequence in fragments shorter than    6952 bp

# AflII.PsiI (CTTAAG+TTATAA) double digest, 2290 thousand fragments (643284+1647912 sites), 1300 bp average fragment length
   5% of sequence in fragments shorter than     438 bp
  10% of sequence in fragments shorter than     676 bp
  15% of sequence in fragments shorter than     888 bp
  20% of sequence in fragments shorter than    1089 bp
  25% of sequence in fragments shorter than    1292 bp
  30% of sequence in fragments shorter than    1483 bp
  35% of sequence in fragments shorter than    1685 bp
  40% of sequence in fragments shorter than    1906 bp
  45% of sequence in fragments shorter than    2139 bp
  50% of sequence in fragments shorter than    2395 bp
  55% of sequence in fragments shorter than    2679 bp
  60% of sequence in fragments shorter than    2988 bp
  65% of sequence in fragments shorter than    3346 bp
  70% of sequence in fragments shorter than    3771 bp
  75% of sequence in fragments shorter than    4295 bp
  80% of sequence in fragments shorter than    4963 bp
  85% of sequence in fragments shorter than    5895 bp
  90% of sequence in fragments shorter than    7453 bp
  95% of sequence in fragments shorter than   11360 bp

# FauI.XbaI (CCCGC+TCTAGA) double digest, 2290 thousand fragments (1439772+850998 sites), 1301 bp average fragment length
   5% of sequence in fragments shorter than     447 bp
  10% of sequence in fragments shorter than     739 bp
  15% of sequence in fragments shorter than     980 bp
  20% of sequence in fragments shorter than    1226 bp
  25% of sequence in fragments shorter than    1458 bp
  30% of sequence in fragments shorter than    1707 bp
  35% of sequence in fragments shorter than    1957 bp
  40% of sequence in fragments shorter than    2214 bp
  45% of sequence in fragments shorter than    2483 bp
  50% of sequence in fragments shorter than    2773 bp
  55% of sequence in fragments shorter than    3071 bp
  60% of sequence in fragments shorter than    3413 bp
  65% of sequence in fragments shorter than    3793 bp
  70% of sequence in fragments shorter than    4222 bp
  75% of sequence in fragments shorter than    4725 bp
  80% of sequence in fragments shorter than    5329 bp
  85% of sequence in fragments shorter than    6107 bp
  90% of sequence in fragments shorter than    7193 bp
  95% of sequence in fragments shorter than    9083 bp

# BclI.HgiCI (TGATCA+GGYRCC) double digest, 2289 thousand fragments (738785+1550877 sites), 1301 bp average fragment length
   5% of sequence in fragments shorter than     441 bp
  10% of sequence in fragments shorter than     673 bp
  15% of sequence in fragments shorter than     874 bp
  20% of sequence in fragments shorter than    1064 bp
  25% of sequence in fragments shorter than    1252 bp
  30% of sequence in fragments shorter than    1448 bp
  35% of sequence in fragments shorter than    1637 bp
  40% of sequence in fragments shorter than    1842 bp
  45% of sequence in fragments shorter than    2058 bp
  50% of sequence in fragments shorter than    2289 bp
  55% of sequence in fragments shorter than    2538 bp
  60% of sequence in fragments shorter than    2809 bp
  65% of sequence in fragments shorter than    3107 bp
  70% of sequence in fragments shorter than    3458 bp
  75% of sequence in fragments shorter than    3870 bp
  80% of sequence in fragments shorter than    4372 bp
  85% of sequence in fragments shorter than    5038 bp
  90% of sequence in fragments shorter than    6008 bp
  95% of sequence in fragments shorter than    8017 bp

# BspMI.XbaI (ACCTGC+TCTAGA) double digest, 2288 thousand fragments (1438129+850998 sites), 1302 bp average fragment length
   5% of sequence in fragments shorter than     448 bp
  10% of sequence in fragments shorter than     677 bp
  15% of sequence in fragments shorter than     882 bp
  20% of sequence in fragments shorter than    1083 bp
  25% of sequence in fragments shorter than    1284 bp
  30% of sequence in fragments shorter than    1468 bp
  35% of sequence in fragments shorter than    1669 bp
  40% of sequence in fragments shorter than    1877 bp
  45% of sequence in fragments shorter than    2072 bp
  50% of sequence in fragments shorter than    2288 bp
  55% of sequence in fragments shorter than    2530 bp
  60% of sequence in fragments shorter than    2789 bp
  65% of sequence in fragments shorter than    3079 bp
  70% of sequence in fragments shorter than    3407 bp
  75% of sequence in fragments shorter than    3791 bp
  80% of sequence in fragments shorter than    4252 bp
  85% of sequence in fragments shorter than    4841 bp
  90% of sequence in fragments shorter than    5664 bp
  95% of sequence in fragments shorter than    7100 bp

# BclI.BsmI (TGATCA+GAATGC) double digest, 2287 thousand fragments (738785+1549350 sites), 1302 bp average fragment length
   5% of sequence in fragments shorter than     424 bp
  10% of sequence in fragments shorter than     731 bp
  15% of sequence in fragments shorter than     976 bp
  20% of sequence in fragments shorter than    1198 bp
  25% of sequence in fragments shorter than    1418 bp
  30% of sequence in fragments shorter than    1636 bp
  35% of sequence in fragments shorter than    1852 bp
  40% of sequence in fragments shorter than    2073 bp
  45% of sequence in fragments shorter than    2302 bp
  50% of sequence in fragments shorter than    2540 bp
  55% of sequence in fragments shorter than    2798 bp
  60% of sequence in fragments shorter than    3082 bp
  65% of sequence in fragments shorter than    3395 bp
  70% of sequence in fragments shorter than    3748 bp
  75% of sequence in fragments shorter than    4157 bp
  80% of sequence in fragments shorter than    4647 bp
  85% of sequence in fragments shorter than    5260 bp
  90% of sequence in fragments shorter than    6120 bp
  95% of sequence in fragments shorter than    7574 bp

# EcoRI.FauI (GAATTC+CCCGC) double digest, 2286 thousand fragments (847401+1439772 sites), 1303 bp average fragment length
   5% of sequence in fragments shorter than     442 bp
  10% of sequence in fragments shorter than     733 bp
  15% of sequence in fragments shorter than    1000 bp
  20% of sequence in fragments shorter than    1250 bp
  25% of sequence in fragments shorter than    1497 bp
  30% of sequence in fragments shorter than    1752 bp
  35% of sequence in fragments shorter than    2011 bp
  40% of sequence in fragments shorter than    2275 bp
  45% of sequence in fragments shorter than    2556 bp
  50% of sequence in fragments shorter than    2851 bp
  55% of sequence in fragments shorter than    3160 bp
  60% of sequence in fragments shorter than    3505 bp
  65% of sequence in fragments shorter than    3903 bp
  70% of sequence in fragments shorter than    4349 bp
  75% of sequence in fragments shorter than    4870 bp
  80% of sequence in fragments shorter than    5508 bp
  85% of sequence in fragments shorter than    6313 bp
  90% of sequence in fragments shorter than    7444 bp
  95% of sequence in fragments shorter than    9420 bp

# BspMI.EcoRI (ACCTGC+GAATTC) double digest, 2285 thousand fragments (1438129+847401 sites), 1304 bp average fragment length
   5% of sequence in fragments shorter than     436 bp
  10% of sequence in fragments shorter than     683 bp
  15% of sequence in fragments shorter than     877 bp
  20% of sequence in fragments shorter than    1079 bp
  25% of sequence in fragments shorter than    1279 bp
  30% of sequence in fragments shorter than    1474 bp
  35% of sequence in fragments shorter than    1676 bp
  40% of sequence in fragments shorter than    1883 bp
  45% of sequence in fragments shorter than    2082 bp
  50% of sequence in fragments shorter than    2309 bp
  55% of sequence in fragments shorter than    2554 bp
  60% of sequence in fragments shorter than    2790 bp
  65% of sequence in fragments shorter than    3080 bp
  70% of sequence in fragments shorter than    3397 bp
  75% of sequence in fragments shorter than    3775 bp
  80% of sequence in fragments shorter than    4227 bp
  85% of sequence in fragments shorter than    4798 bp
  90% of sequence in fragments shorter than    5597 bp
  95% of sequence in fragments shorter than    6933 bp

# FnuDII.HgiCI (CGCG+GGYRCC) double digest, 2284 thousand fragments (733940+1550877 sites), 1304 bp average fragment length
   5% of sequence in fragments shorter than     446 bp
  10% of sequence in fragments shorter than     755 bp
  15% of sequence in fragments shorter than    1037 bp
  20% of sequence in fragments shorter than    1287 bp
  25% of sequence in fragments shorter than    1561 bp
  30% of sequence in fragments shorter than    1831 bp
  35% of sequence in fragments shorter than    2127 bp
  40% of sequence in fragments shorter than    2440 bp
  45% of sequence in fragments shorter than    2767 bp
  50% of sequence in fragments shorter than    3108 bp
  55% of sequence in fragments shorter than    3502 bp
  60% of sequence in fragments shorter than    3941 bp
  65% of sequence in fragments shorter than    4431 bp
  70% of sequence in fragments shorter than    5005 bp
  75% of sequence in fragments shorter than    5688 bp
  80% of sequence in fragments shorter than    6532 bp
  85% of sequence in fragments shorter than    7664 bp
  90% of sequence in fragments shorter than    9331 bp
  95% of sequence in fragments shorter than   12672 bp

# BsmI.FnuDII (GAATGC+CGCG) double digest, 2282 thousand fragments (1549350+733940 sites), 1305 bp average fragment length
   5% of sequence in fragments shorter than     430 bp
  10% of sequence in fragments shorter than     799 bp
  15% of sequence in fragments shorter than    1092 bp
  20% of sequence in fragments shorter than    1366 bp
  25% of sequence in fragments shorter than    1635 bp
  30% of sequence in fragments shorter than    1896 bp
  35% of sequence in fragments shorter than    2160 bp
  40% of sequence in fragments shorter than    2432 bp
  45% of sequence in fragments shorter than    2711 bp
  50% of sequence in fragments shorter than    3001 bp
  55% of sequence in fragments shorter than    3317 bp
  60% of sequence in fragments shorter than    3665 bp
  65% of sequence in fragments shorter than    4049 bp
  70% of sequence in fragments shorter than    4477 bp
  75% of sequence in fragments shorter than    4975 bp
  80% of sequence in fragments shorter than    5573 bp
  85% of sequence in fragments shorter than    6315 bp
  90% of sequence in fragments shorter than    7350 bp
  95% of sequence in fragments shorter than    9086 bp

# BfiI.HgaI (ACTGGG+GACGC) double digest, 2278 thousand fragments (1706594+571889 sites), 1308 bp average fragment length
   5% of sequence in fragments shorter than     427 bp
  10% of sequence in fragments shorter than     682 bp
  15% of sequence in fragments shorter than     906 bp
  20% of sequence in fragments shorter than    1116 bp
  25% of sequence in fragments shorter than    1332 bp
  30% of sequence in fragments shorter than    1547 bp
  35% of sequence in fragments shorter than    1767 bp
  40% of sequence in fragments shorter than    1996 bp
  45% of sequence in fragments shorter than    2223 bp
  50% of sequence in fragments shorter than    2478 bp
  55% of sequence in fragments shorter than    2757 bp
  60% of sequence in fragments shorter than    3051 bp
  65% of sequence in fragments shorter than    3397 bp
  70% of sequence in fragments shorter than    3795 bp
  75% of sequence in fragments shorter than    4261 bp
  80% of sequence in fragments shorter than    4828 bp
  85% of sequence in fragments shorter than    5545 bp
  90% of sequence in fragments shorter than    6598 bp
  95% of sequence in fragments shorter than    8533 bp

# HaeII.PsiI (RGCGCY+TTATAA) double digest, 2277 thousand fragments (630101+1647912 sites), 1308 bp average fragment length
   5% of sequence in fragments shorter than     441 bp
  10% of sequence in fragments shorter than     688 bp
  15% of sequence in fragments shorter than     896 bp
  20% of sequence in fragments shorter than    1102 bp
  25% of sequence in fragments shorter than    1310 bp
  30% of sequence in fragments shorter than    1516 bp
  35% of sequence in fragments shorter than    1725 bp
  40% of sequence in fragments shorter than    1941 bp
  45% of sequence in fragments shorter than    2163 bp
  50% of sequence in fragments shorter than    2403 bp
  55% of sequence in fragments shorter than    2667 bp
  60% of sequence in fragments shorter than    2945 bp
  65% of sequence in fragments shorter than    3260 bp
  70% of sequence in fragments shorter than    3614 bp
  75% of sequence in fragments shorter than    4024 bp
  80% of sequence in fragments shorter than    4519 bp
  85% of sequence in fragments shorter than    5156 bp
  90% of sequence in fragments shorter than    6052 bp
  95% of sequence in fragments shorter than    7604 bp

# BfiI.EciI (ACTGGG+GGCGGA) double digest, 2277 thousand fragments (1706594+571273 sites), 1308 bp average fragment length
   5% of sequence in fragments shorter than     428 bp
  10% of sequence in fragments shorter than     678 bp
  15% of sequence in fragments shorter than     894 bp
  20% of sequence in fragments shorter than    1095 bp
  25% of sequence in fragments shorter than    1300 bp
  30% of sequence in fragments shorter than    1508 bp
  35% of sequence in fragments shorter than    1722 bp
  40% of sequence in fragments shorter than    1943 bp
  45% of sequence in fragments shorter than    2170 bp
  50% of sequence in fragments shorter than    2414 bp
  55% of sequence in fragments shorter than    2688 bp
  60% of sequence in fragments shorter than    2958 bp
  65% of sequence in fragments shorter than    3297 bp
  70% of sequence in fragments shorter than    3686 bp
  75% of sequence in fragments shorter than    4152 bp
  80% of sequence in fragments shorter than    4714 bp
  85% of sequence in fragments shorter than    5429 bp
  90% of sequence in fragments shorter than    6506 bp
  95% of sequence in fragments shorter than    8611 bp

# BfiI.MfeI (ACTGGG+CAATTG) double digest, 2270 thousand fragments (1706594+564303 sites), 1312 bp average fragment length
   5% of sequence in fragments shorter than     452 bp
  10% of sequence in fragments shorter than     690 bp
  15% of sequence in fragments shorter than     896 bp
  20% of sequence in fragments shorter than    1086 bp
  25% of sequence in fragments shorter than    1267 bp
  30% of sequence in fragments shorter than    1457 bp
  35% of sequence in fragments shorter than    1638 bp
  40% of sequence in fragments shorter than    1838 bp
  45% of sequence in fragments shorter than    2044 bp
  50% of sequence in fragments shorter than    2249 bp
  55% of sequence in fragments shorter than    2487 bp
  60% of sequence in fragments shorter than    2747 bp
  65% of sequence in fragments shorter than    3032 bp
  70% of sequence in fragments shorter than    3358 bp
  75% of sequence in fragments shorter than    3734 bp
  80% of sequence in fragments shorter than    4191 bp
  85% of sequence in fragments shorter than    4782 bp
  90% of sequence in fragments shorter than    5625 bp
  95% of sequence in fragments shorter than    7175 bp

# BsaAI.HgiCI (YACGTR+GGYRCC) double digest, 2264 thousand fragments (713801+1550877 sites), 1316 bp average fragment length
   5% of sequence in fragments shorter than     443 bp
  10% of sequence in fragments shorter than     685 bp
  15% of sequence in fragments shorter than     898 bp
  20% of sequence in fragments shorter than    1093 bp
  25% of sequence in fragments shorter than    1286 bp
  30% of sequence in fragments shorter than    1485 bp
  35% of sequence in fragments shorter than    1699 bp
  40% of sequence in fragments shorter than    1923 bp
  45% of sequence in fragments shorter than    2152 bp
  50% of sequence in fragments shorter than    2407 bp
  55% of sequence in fragments shorter than    2678 bp
  60% of sequence in fragments shorter than    2970 bp
  65% of sequence in fragments shorter than    3306 bp
  70% of sequence in fragments shorter than    3689 bp
  75% of sequence in fragments shorter than    4146 bp
  80% of sequence in fragments shorter than    4700 bp
  85% of sequence in fragments shorter than    5425 bp
  90% of sequence in fragments shorter than    6484 bp
  95% of sequence in fragments shorter than    8430 bp

# BsaAI.BsmI (YACGTR+GAATGC) double digest, 2262 thousand fragments (713801+1549350 sites), 1317 bp average fragment length
   5% of sequence in fragments shorter than     437 bp
  10% of sequence in fragments shorter than     745 bp
  15% of sequence in fragments shorter than     993 bp
  20% of sequence in fragments shorter than    1218 bp
  25% of sequence in fragments shorter than    1432 bp
  30% of sequence in fragments shorter than    1657 bp
  35% of sequence in fragments shorter than    1876 bp
  40% of sequence in fragments shorter than    2107 bp
  45% of sequence in fragments shorter than    2349 bp
  50% of sequence in fragments shorter than    2598 bp
  55% of sequence in fragments shorter than    2867 bp
  60% of sequence in fragments shorter than    3159 bp
  65% of sequence in fragments shorter than    3481 bp
  70% of sequence in fragments shorter than    3844 bp
  75% of sequence in fragments shorter than    4262 bp
  80% of sequence in fragments shorter than    4761 bp
  85% of sequence in fragments shorter than    5389 bp
  90% of sequence in fragments shorter than    6258 bp
  95% of sequence in fragments shorter than    7724 bp

# AflIII.BglII (ACRYGT+AGATCT) double digest, 2259 thousand fragments (1485394+774732 sites), 1318 bp average fragment length
   5% of sequence in fragments shorter than     465 bp
  10% of sequence in fragments shorter than     719 bp
  15% of sequence in fragments shorter than     935 bp
  20% of sequence in fragments shorter than    1139 bp
  25% of sequence in fragments shorter than    1337 bp
  30% of sequence in fragments shorter than    1530 bp
  35% of sequence in fragments shorter than    1727 bp
  40% of sequence in fragments shorter than    1931 bp
  45% of sequence in fragments shorter than    2140 bp
  50% of sequence in fragments shorter than    2366 bp
  55% of sequence in fragments shorter than    2608 bp
  60% of sequence in fragments shorter than    2858 bp
  65% of sequence in fragments shorter than    3142 bp
  70% of sequence in fragments shorter than    3453 bp
  75% of sequence in fragments shorter than    3837 bp
  80% of sequence in fragments shorter than    4304 bp
  85% of sequence in fragments shorter than    4907 bp
  90% of sequence in fragments shorter than    5774 bp
  95% of sequence in fragments shorter than    7413 bp

# BfiI.SphI (ACTGGG+GCATGC) double digest, 2257 thousand fragments (1706594+550952 sites), 1320 bp average fragment length
   5% of sequence in fragments shorter than     445 bp
  10% of sequence in fragments shorter than     690 bp
  15% of sequence in fragments shorter than     901 bp
  20% of sequence in fragments shorter than    1097 bp
  25% of sequence in fragments shorter than    1297 bp
  30% of sequence in fragments shorter than    1496 bp
  35% of sequence in fragments shorter than    1699 bp
  40% of sequence in fragments shorter than    1906 bp
  45% of sequence in fragments shorter than    2122 bp
  50% of sequence in fragments shorter than    2352 bp
  55% of sequence in fragments shorter than    2608 bp
  60% of sequence in fragments shorter than    2862 bp
  65% of sequence in fragments shorter than    3174 bp
  70% of sequence in fragments shorter than    3530 bp
  75% of sequence in fragments shorter than    3953 bp
  80% of sequence in fragments shorter than    4461 bp
  85% of sequence in fragments shorter than    5106 bp
  90% of sequence in fragments shorter than    6046 bp
  95% of sequence in fragments shorter than    7880 bp

# BceAI.HgiCI (ACGGC+GGYRCC) double digest, 2255 thousand fragments (705178+1550877 sites), 1321 bp average fragment length
   5% of sequence in fragments shorter than     431 bp
  10% of sequence in fragments shorter than     691 bp
  15% of sequence in fragments shorter than     931 bp
  20% of sequence in fragments shorter than    1164 bp
  25% of sequence in fragments shorter than    1379 bp
  30% of sequence in fragments shorter than    1609 bp
  35% of sequence in fragments shorter than    1861 bp
  40% of sequence in fragments shorter than    2123 bp
  45% of sequence in fragments shorter than    2406 bp
  50% of sequence in fragments shorter than    2707 bp
  55% of sequence in fragments shorter than    3022 bp
  60% of sequence in fragments shorter than    3385 bp
  65% of sequence in fragments shorter than    3803 bp
  70% of sequence in fragments shorter than    4272 bp
  75% of sequence in fragments shorter than    4836 bp
  80% of sequence in fragments shorter than    5534 bp
  85% of sequence in fragments shorter than    6445 bp
  90% of sequence in fragments shorter than    7800 bp
  95% of sequence in fragments shorter than   10391 bp

# AvaIII.PstI (ATGCAT+CTGCAG) double digest, 2254 thousand fragments (933357+1321469 sites), 1321 bp average fragment length
   5% of sequence in fragments shorter than     459 bp
  10% of sequence in fragments shorter than     694 bp
  15% of sequence in fragments shorter than     907 bp
  20% of sequence in fragments shorter than    1106 bp
  25% of sequence in fragments shorter than    1301 bp
  30% of sequence in fragments shorter than    1492 bp
  35% of sequence in fragments shorter than    1692 bp
  40% of sequence in fragments shorter than    1900 bp
  45% of sequence in fragments shorter than    2112 bp
  50% of sequence in fragments shorter than    2345 bp
  55% of sequence in fragments shorter than    2596 bp
  60% of sequence in fragments shorter than    2872 bp
  65% of sequence in fragments shorter than    3176 bp
  70% of sequence in fragments shorter than    3523 bp
  75% of sequence in fragments shorter than    3931 bp
  80% of sequence in fragments shorter than    4423 bp
  85% of sequence in fragments shorter than    5041 bp
  90% of sequence in fragments shorter than    5907 bp
  95% of sequence in fragments shorter than    7475 bp

# BceAI.BsmI (ACGGC+GAATGC) double digest, 2254 thousand fragments (705178+1549350 sites), 1322 bp average fragment length
   5% of sequence in fragments shorter than     423 bp
  10% of sequence in fragments shorter than     736 bp
  15% of sequence in fragments shorter than     995 bp
  20% of sequence in fragments shorter than    1234 bp
  25% of sequence in fragments shorter than    1472 bp
  30% of sequence in fragments shorter than    1709 bp
  35% of sequence in fragments shorter than    1948 bp
  40% of sequence in fragments shorter than    2194 bp
  45% of sequence in fragments shorter than    2450 bp
  50% of sequence in fragments shorter than    2716 bp
  55% of sequence in fragments shorter than    2997 bp
  60% of sequence in fragments shorter than    3310 bp
  65% of sequence in fragments shorter than    3659 bp
  70% of sequence in fragments shorter than    4047 bp
  75% of sequence in fragments shorter than    4494 bp
  80% of sequence in fragments shorter than    5041 bp
  85% of sequence in fragments shorter than    5734 bp
  90% of sequence in fragments shorter than    6680 bp
  95% of sequence in fragments shorter than    8289 bp

# ApaLI.HhaI (GTGCAC+GCGC) double digest, 2252 thousand fragments (496255+1756502 sites), 1323 bp average fragment length
   5% of sequence in fragments shorter than     437 bp
  10% of sequence in fragments shorter than     746 bp
  15% of sequence in fragments shorter than    1041 bp
  20% of sequence in fragments shorter than    1347 bp
  25% of sequence in fragments shorter than    1650 bp
  30% of sequence in fragments shorter than    1957 bp
  35% of sequence in fragments shorter than    2288 bp
  40% of sequence in fragments shorter than    2638 bp
  45% of sequence in fragments shorter than    3017 bp
  50% of sequence in fragments shorter than    3426 bp
  55% of sequence in fragments shorter than    3876 bp
  60% of sequence in fragments shorter than    4361 bp
  65% of sequence in fragments shorter than    4925 bp
  70% of sequence in fragments shorter than    5542 bp
  75% of sequence in fragments shorter than    6248 bp
  80% of sequence in fragments shorter than    7158 bp
  85% of sequence in fragments shorter than    8339 bp
  90% of sequence in fragments shorter than    9994 bp
  95% of sequence in fragments shorter than   12870 bp

# PsiI.SacI (TTATAA+GAGCTC) double digest, 2250 thousand fragments (1647912+603323 sites), 1323 bp average fragment length
   5% of sequence in fragments shorter than     460 bp
  10% of sequence in fragments shorter than     702 bp
  15% of sequence in fragments shorter than     909 bp
  20% of sequence in fragments shorter than    1110 bp
  25% of sequence in fragments shorter than    1305 bp
  30% of sequence in fragments shorter than    1500 bp
  35% of sequence in fragments shorter than    1703 bp
  40% of sequence in fragments shorter than    1911 bp
  45% of sequence in fragments shorter than    2126 bp
  50% of sequence in fragments shorter than    2360 bp
  55% of sequence in fragments shorter than    2611 bp
  60% of sequence in fragments shorter than    2890 bp
  65% of sequence in fragments shorter than    3185 bp
  70% of sequence in fragments shorter than    3535 bp
  75% of sequence in fragments shorter than    3932 bp
  80% of sequence in fragments shorter than    4423 bp
  85% of sequence in fragments shorter than    5069 bp
  90% of sequence in fragments shorter than    6015 bp
  95% of sequence in fragments shorter than    7840 bp

# BfiI.ScaI (ACTGGG+AGTACT) double digest, 2249 thousand fragments (1706594+543793 sites), 1324 bp average fragment length
   5% of sequence in fragments shorter than     460 bp
  10% of sequence in fragments shorter than     708 bp
  15% of sequence in fragments shorter than     920 bp
  20% of sequence in fragments shorter than    1116 bp
  25% of sequence in fragments shorter than    1313 bp
  30% of sequence in fragments shorter than    1508 bp
  35% of sequence in fragments shorter than    1707 bp
  40% of sequence in fragments shorter than    1908 bp
  45% of sequence in fragments shorter than    2115 bp
  50% of sequence in fragments shorter than    2335 bp
  55% of sequence in fragments shorter than    2578 bp
  60% of sequence in fragments shorter than    2817 bp
  65% of sequence in fragments shorter than    3112 bp
  70% of sequence in fragments shorter than    3445 bp
  75% of sequence in fragments shorter than    3833 bp
  80% of sequence in fragments shorter than    4308 bp
  85% of sequence in fragments shorter than    4906 bp
  90% of sequence in fragments shorter than    5765 bp
  95% of sequence in fragments shorter than    7428 bp

# PstI.StuI (CTGCAG+AGGCCT) double digest, 2246 thousand fragments (1321469+925729 sites), 1326 bp average fragment length
   5% of sequence in fragments shorter than     415 bp
  10% of sequence in fragments shorter than     672 bp
  15% of sequence in fragments shorter than     891 bp
  20% of sequence in fragments shorter than    1120 bp
  25% of sequence in fragments shorter than    1357 bp
  30% of sequence in fragments shorter than    1591 bp
  35% of sequence in fragments shorter than    1839 bp
  40% of sequence in fragments shorter than    2080 bp
  45% of sequence in fragments shorter than    2354 bp
  50% of sequence in fragments shorter than    2648 bp
  55% of sequence in fragments shorter than    2964 bp
  60% of sequence in fragments shorter than    3312 bp
  65% of sequence in fragments shorter than    3704 bp
  70% of sequence in fragments shorter than    4150 bp
  75% of sequence in fragments shorter than    4671 bp
  80% of sequence in fragments shorter than    5301 bp
  85% of sequence in fragments shorter than    6100 bp
  90% of sequence in fragments shorter than    7227 bp
  95% of sequence in fragments shorter than    9134 bp

# BciVI.HgiCI (GTATCC+GGYRCC) double digest, 2246 thousand fragments (696093+1550877 sites), 1326 bp average fragment length
   5% of sequence in fragments shorter than     447 bp
  10% of sequence in fragments shorter than     684 bp
  15% of sequence in fragments shorter than     889 bp
  20% of sequence in fragments shorter than    1093 bp
  25% of sequence in fragments shorter than    1289 bp
  30% of sequence in fragments shorter than    1487 bp
  35% of sequence in fragments shorter than    1690 bp
  40% of sequence in fragments shorter than    1901 bp
  45% of sequence in fragments shorter than    2121 bp
  50% of sequence in fragments shorter than    2359 bp
  55% of sequence in fragments shorter than    2618 bp
  60% of sequence in fragments shorter than    2898 bp
  65% of sequence in fragments shorter than    3212 bp
  70% of sequence in fragments shorter than    3574 bp
  75% of sequence in fragments shorter than    3998 bp
  80% of sequence in fragments shorter than    4515 bp
  85% of sequence in fragments shorter than    5198 bp
  90% of sequence in fragments shorter than    6179 bp
  95% of sequence in fragments shorter than    8068 bp

# AflII.EsaBC3I (CTTAAG+TCGA) double digest, 2246 thousand fragments (643284+1603346 sites), 1326 bp average fragment length
   5% of sequence in fragments shorter than     460 bp
  10% of sequence in fragments shorter than     697 bp
  15% of sequence in fragments shorter than     899 bp
  20% of sequence in fragments shorter than    1094 bp
  25% of sequence in fragments shorter than    1287 bp
  30% of sequence in fragments shorter than    1473 bp
  35% of sequence in fragments shorter than    1671 bp
  40% of sequence in fragments shorter than    1874 bp
  45% of sequence in fragments shorter than    2086 bp
  50% of sequence in fragments shorter than    2307 bp
  55% of sequence in fragments shorter than    2547 bp
  60% of sequence in fragments shorter than    2805 bp
  65% of sequence in fragments shorter than    3094 bp
  70% of sequence in fragments shorter than    3420 bp
  75% of sequence in fragments shorter than    3799 bp
  80% of sequence in fragments shorter than    4254 bp
  85% of sequence in fragments shorter than    4837 bp
  90% of sequence in fragments shorter than    5665 bp
  95% of sequence in fragments shorter than    7114 bp

# BciVI.BsmI (GTATCC+GAATGC) double digest, 2244 thousand fragments (696093+1549350 sites), 1327 bp average fragment length
   5% of sequence in fragments shorter than     437 bp
  10% of sequence in fragments shorter than     751 bp
  15% of sequence in fragments shorter than    1003 bp
  20% of sequence in fragments shorter than    1235 bp
  25% of sequence in fragments shorter than    1460 bp
  30% of sequence in fragments shorter than    1685 bp
  35% of sequence in fragments shorter than    1907 bp
  40% of sequence in fragments shorter than    2137 bp
  45% of sequence in fragments shorter than    2378 bp
  50% of sequence in fragments shorter than    2628 bp
  55% of sequence in fragments shorter than    2897 bp
  60% of sequence in fragments shorter than    3192 bp
  65% of sequence in fragments shorter than    3518 bp
  70% of sequence in fragments shorter than    3885 bp
  75% of sequence in fragments shorter than    4304 bp
  80% of sequence in fragments shorter than    4807 bp
  85% of sequence in fragments shorter than    5446 bp
  90% of sequence in fragments shorter than    6337 bp
  95% of sequence in fragments shorter than    7841 bp

# AflIII.NcoI (ACRYGT+CCATGG) double digest, 2244 thousand fragments (1485394+759594 sites), 1327 bp average fragment length
   5% of sequence in fragments shorter than     466 bp
  10% of sequence in fragments shorter than     735 bp
  15% of sequence in fragments shorter than     959 bp
  20% of sequence in fragments shorter than    1172 bp
  25% of sequence in fragments shorter than    1379 bp
  30% of sequence in fragments shorter than    1582 bp
  35% of sequence in fragments shorter than    1793 bp
  40% of sequence in fragments shorter than    2008 bp
  45% of sequence in fragments shorter than    2230 bp
  50% of sequence in fragments shorter than    2469 bp
  55% of sequence in fragments shorter than    2726 bp
  60% of sequence in fragments shorter than    3000 bp
  65% of sequence in fragments shorter than    3308 bp
  70% of sequence in fragments shorter than    3660 bp
  75% of sequence in fragments shorter than    4066 bp
  80% of sequence in fragments shorter than    4552 bp
  85% of sequence in fragments shorter than    5191 bp
  90% of sequence in fragments shorter than    6067 bp
  95% of sequence in fragments shorter than    7680 bp

# AvrII.PsiI (CCTAGG+TTATAA) double digest, 2242 thousand fragments (594956+1647912 sites), 1328 bp average fragment length
   5% of sequence in fragments shorter than     460 bp
  10% of sequence in fragments shorter than     685 bp
  15% of sequence in fragments shorter than     901 bp
  20% of sequence in fragments shorter than    1105 bp
  25% of sequence in fragments shorter than    1303 bp
  30% of sequence in fragments shorter than    1502 bp
  35% of sequence in fragments shorter than    1699 bp
  40% of sequence in fragments shorter than    1915 bp
  45% of sequence in fragments shorter than    2143 bp
  50% of sequence in fragments shorter than    2384 bp
  55% of sequence in fragments shorter than    2649 bp
  60% of sequence in fragments shorter than    2939 bp
  65% of sequence in fragments shorter than    3259 bp
  70% of sequence in fragments shorter than    3637 bp
  75% of sequence in fragments shorter than    4086 bp
  80% of sequence in fragments shorter than    4645 bp
  85% of sequence in fragments shorter than    5380 bp
  90% of sequence in fragments shorter than    6494 bp
  95% of sequence in fragments shorter than    8807 bp

# AvaI.BscGI (CYCGRG+CCCGT) double digest, 2236 thousand fragments (1005714+1231070 sites), 1332 bp average fragment length
   5% of sequence in fragments shorter than     367 bp
  10% of sequence in fragments shorter than     676 bp
  15% of sequence in fragments shorter than     951 bp
  20% of sequence in fragments shorter than    1238 bp
  25% of sequence in fragments shorter than    1537 bp
  30% of sequence in fragments shorter than    1855 bp
  35% of sequence in fragments shorter than    2194 bp
  40% of sequence in fragments shorter than    2559 bp
  45% of sequence in fragments shorter than    2954 bp
  50% of sequence in fragments shorter than    3386 bp
  55% of sequence in fragments shorter than    3862 bp
  60% of sequence in fragments shorter than    4390 bp
  65% of sequence in fragments shorter than    4994 bp
  70% of sequence in fragments shorter than    5687 bp
  75% of sequence in fragments shorter than    6509 bp
  80% of sequence in fragments shorter than    7511 bp
  85% of sequence in fragments shorter than    8808 bp
  90% of sequence in fragments shorter than   10670 bp
  95% of sequence in fragments shorter than   13887 bp

# EsaBC3I.HaeII (TCGA+RGCGCY) double digest, 2232 thousand fragments (1603346+630101 sites), 1334 bp average fragment length
   5% of sequence in fragments shorter than     430 bp
  10% of sequence in fragments shorter than     690 bp
  15% of sequence in fragments shorter than     933 bp
  20% of sequence in fragments shorter than    1164 bp
  25% of sequence in fragments shorter than    1396 bp
  30% of sequence in fragments shorter than    1638 bp
  35% of sequence in fragments shorter than    1886 bp
  40% of sequence in fragments shorter than    2146 bp
  45% of sequence in fragments shorter than    2422 bp
  50% of sequence in fragments shorter than    2718 bp
  55% of sequence in fragments shorter than    3035 bp
  60% of sequence in fragments shorter than    3384 bp
  65% of sequence in fragments shorter than    3773 bp
  70% of sequence in fragments shorter than    4220 bp
  75% of sequence in fragments shorter than    4747 bp
  80% of sequence in fragments shorter than    5384 bp
  85% of sequence in fragments shorter than    6194 bp
  90% of sequence in fragments shorter than    7340 bp
  95% of sequence in fragments shorter than    9242 bp

# NdeI.PstI (CATATG+CTGCAG) double digest, 2224 thousand fragments (903360+1321469 sites), 1339 bp average fragment length
   5% of sequence in fragments shorter than     463 bp
  10% of sequence in fragments shorter than     707 bp
  15% of sequence in fragments shorter than     914 bp
  20% of sequence in fragments shorter than    1112 bp
  25% of sequence in fragments shorter than    1296 bp
  30% of sequence in fragments shorter than    1489 bp
  35% of sequence in fragments shorter than    1689 bp
  40% of sequence in fragments shorter than    1896 bp
  45% of sequence in fragments shorter than    2104 bp
  50% of sequence in fragments shorter than    2330 bp
  55% of sequence in fragments shorter than    2576 bp
  60% of sequence in fragments shorter than    2840 bp
  65% of sequence in fragments shorter than    3124 bp
  70% of sequence in fragments shorter than    3434 bp
  75% of sequence in fragments shorter than    3810 bp
  80% of sequence in fragments shorter than    4266 bp
  85% of sequence in fragments shorter than    4863 bp
  90% of sequence in fragments shorter than    5706 bp
  95% of sequence in fragments shorter than    7241 bp

# AflIII.BclI (ACRYGT+TGATCA) double digest, 2223 thousand fragments (1485394+738785 sites), 1340 bp average fragment length
   5% of sequence in fragments shorter than     471 bp
  10% of sequence in fragments shorter than     730 bp
  15% of sequence in fragments shorter than     951 bp
  20% of sequence in fragments shorter than    1160 bp
  25% of sequence in fragments shorter than    1361 bp
  30% of sequence in fragments shorter than    1562 bp
  35% of sequence in fragments shorter than    1765 bp
  40% of sequence in fragments shorter than    1960 bp
  45% of sequence in fragments shorter than    2175 bp
  50% of sequence in fragments shorter than    2402 bp
  55% of sequence in fragments shorter than    2647 bp
  60% of sequence in fragments shorter than    2913 bp
  65% of sequence in fragments shorter than    3209 bp
  70% of sequence in fragments shorter than    3553 bp
  75% of sequence in fragments shorter than    3953 bp
  80% of sequence in fragments shorter than    4425 bp
  85% of sequence in fragments shorter than    5035 bp
  90% of sequence in fragments shorter than    5914 bp
  95% of sequence in fragments shorter than    7589 bp

# HgaI.PsiI (GACGC+TTATAA) double digest, 2219 thousand fragments (571889+1647912 sites), 1342 bp average fragment length
   5% of sequence in fragments shorter than     462 bp
  10% of sequence in fragments shorter than     715 bp
  15% of sequence in fragments shorter than     938 bp
  20% of sequence in fragments shorter than    1149 bp
  25% of sequence in fragments shorter than    1355 bp
  30% of sequence in fragments shorter than    1559 bp
  35% of sequence in fragments shorter than    1771 bp
  40% of sequence in fragments shorter than    1987 bp
  45% of sequence in fragments shorter than    2218 bp
  50% of sequence in fragments shorter than    2457 bp
  55% of sequence in fragments shorter than    2721 bp
  60% of sequence in fragments shorter than    2995 bp
  65% of sequence in fragments shorter than    3312 bp
  70% of sequence in fragments shorter than    3671 bp
  75% of sequence in fragments shorter than    4094 bp
  80% of sequence in fragments shorter than    4608 bp
  85% of sequence in fragments shorter than    5284 bp
  90% of sequence in fragments shorter than    6245 bp
  95% of sequence in fragments shorter than    8107 bp

# AflIII.FnuDII (ACRYGT+CGCG) double digest, 2218 thousand fragments (1485394+733940 sites), 1343 bp average fragment length
   5% of sequence in fragments shorter than     492 bp
  10% of sequence in fragments shorter than     806 bp
  15% of sequence in fragments shorter than    1077 bp
  20% of sequence in fragments shorter than    1327 bp
  25% of sequence in fragments shorter than    1574 bp
  30% of sequence in fragments shorter than    1818 bp
  35% of sequence in fragments shorter than    2063 bp
  40% of sequence in fragments shorter than    2310 bp
  45% of sequence in fragments shorter than    2577 bp
  50% of sequence in fragments shorter than    2855 bp
  55% of sequence in fragments shorter than    3151 bp
  60% of sequence in fragments shorter than    3481 bp
  65% of sequence in fragments shorter than    3847 bp
  70% of sequence in fragments shorter than    4264 bp
  75% of sequence in fragments shorter than    4748 bp
  80% of sequence in fragments shorter than    5329 bp
  85% of sequence in fragments shorter than    6046 bp
  90% of sequence in fragments shorter than    7089 bp
  95% of sequence in fragments shorter than    8983 bp

# EciI.PsiI (GGCGGA+TTATAA) double digest, 2218 thousand fragments (571273+1647912 sites), 1343 bp average fragment length
   5% of sequence in fragments shorter than     452 bp
  10% of sequence in fragments shorter than     702 bp
  15% of sequence in fragments shorter than     921 bp
  20% of sequence in fragments shorter than    1129 bp
  25% of sequence in fragments shorter than    1335 bp
  30% of sequence in fragments shorter than    1541 bp
  35% of sequence in fragments shorter than    1753 bp
  40% of sequence in fragments shorter than    1974 bp
  45% of sequence in fragments shorter than    2207 bp
  50% of sequence in fragments shorter than    2457 bp
  55% of sequence in fragments shorter than    2730 bp
  60% of sequence in fragments shorter than    3016 bp
  65% of sequence in fragments shorter than    3348 bp
  70% of sequence in fragments shorter than    3728 bp
  75% of sequence in fragments shorter than    4174 bp
  80% of sequence in fragments shorter than    4737 bp
  85% of sequence in fragments shorter than    5470 bp
  90% of sequence in fragments shorter than    6572 bp
  95% of sequence in fragments shorter than    8817 bp

# ApaI.HhaI (GGGCCC+GCGC) double digest, 2216 thousand fragments (460339+1756502 sites), 1344 bp average fragment length
   5% of sequence in fragments shorter than     425 bp
  10% of sequence in fragments shorter than     750 bp
  15% of sequence in fragments shorter than    1074 bp
  20% of sequence in fragments shorter than    1413 bp
  25% of sequence in fragments shorter than    1764 bp
  30% of sequence in fragments shorter than    2127 bp
  35% of sequence in fragments shorter than    2526 bp
  40% of sequence in fragments shorter than    2953 bp
  45% of sequence in fragments shorter than    3418 bp
  50% of sequence in fragments shorter than    3925 bp
  55% of sequence in fragments shorter than    4487 bp
  60% of sequence in fragments shorter than    5118 bp
  65% of sequence in fragments shorter than    5821 bp
  70% of sequence in fragments shorter than    6643 bp
  75% of sequence in fragments shorter than    7619 bp
  80% of sequence in fragments shorter than    8802 bp
  85% of sequence in fragments shorter than   10351 bp
  90% of sequence in fragments shorter than   12540 bp
  95% of sequence in fragments shorter than   16308 bp

# BglII.FauI (AGATCT+CCCGC) double digest, 2214 thousand fragments (774732+1439772 sites), 1345 bp average fragment length
   5% of sequence in fragments shorter than     472 bp
  10% of sequence in fragments shorter than     769 bp
  15% of sequence in fragments shorter than    1039 bp
  20% of sequence in fragments shorter than    1297 bp
  25% of sequence in fragments shorter than    1540 bp
  30% of sequence in fragments shorter than    1787 bp
  35% of sequence in fragments shorter than    2048 bp
  40% of sequence in fragments shorter than    2318 bp
  45% of sequence in fragments shorter than    2598 bp
  50% of sequence in fragments shorter than    2909 bp
  55% of sequence in fragments shorter than    3245 bp
  60% of sequence in fragments shorter than    3612 bp
  65% of sequence in fragments shorter than    4021 bp
  70% of sequence in fragments shorter than    4490 bp
  75% of sequence in fragments shorter than    5039 bp
  80% of sequence in fragments shorter than    5706 bp
  85% of sequence in fragments shorter than    6576 bp
  90% of sequence in fragments shorter than    7842 bp
  95% of sequence in fragments shorter than   10283 bp

# BglII.BspMI (AGATCT+ACCTGC) double digest, 2212 thousand fragments (774732+1438129 sites), 1346 bp average fragment length
   5% of sequence in fragments shorter than     462 bp
  10% of sequence in fragments shorter than     703 bp
  15% of sequence in fragments shorter than     913 bp
  20% of sequence in fragments shorter than    1117 bp
  25% of sequence in fragments shorter than    1319 bp
  30% of sequence in fragments shorter than    1505 bp
  35% of sequence in fragments shorter than    1710 bp
  40% of sequence in fragments shorter than    1922 bp
  45% of sequence in fragments shorter than    2136 bp
  50% of sequence in fragments shorter than    2366 bp
  55% of sequence in fragments shorter than    2618 bp
  60% of sequence in fragments shorter than    2886 bp
  65% of sequence in fragments shorter than    3168 bp
  70% of sequence in fragments shorter than    3479 bp
  75% of sequence in fragments shorter than    3868 bp
  80% of sequence in fragments shorter than    4340 bp
  85% of sequence in fragments shorter than    4937 bp
  90% of sequence in fragments shorter than    5790 bp
  95% of sequence in fragments shorter than    7278 bp

# MfeI.PsiI (CAATTG+TTATAA) double digest, 2211 thousand fragments (564303+1647912 sites), 1347 bp average fragment length
   5% of sequence in fragments shorter than     454 bp
  10% of sequence in fragments shorter than     698 bp
  15% of sequence in fragments shorter than     915 bp
  20% of sequence in fragments shorter than    1125 bp
  25% of sequence in fragments shorter than    1327 bp
  30% of sequence in fragments shorter than    1541 bp
  35% of sequence in fragments shorter than    1753 bp
  40% of sequence in fragments shorter than    1984 bp
  45% of sequence in fragments shorter than    2232 bp
  50% of sequence in fragments shorter than    2505 bp
  55% of sequence in fragments shorter than    2807 bp
  60% of sequence in fragments shorter than    3131 bp
  65% of sequence in fragments shorter than    3517 bp
  70% of sequence in fragments shorter than    3978 bp
  75% of sequence in fragments shorter than    4544 bp
  80% of sequence in fragments shorter than    5276 bp
  85% of sequence in fragments shorter than    6309 bp
  90% of sequence in fragments shorter than    8048 bp
  95% of sequence in fragments shorter than   12325 bp

# BscGI.BspHI (CCCGT+TCATGA) double digest, 2208 thousand fragments (1231070+978290 sites), 1349 bp average fragment length
   5% of sequence in fragments shorter than     427 bp
  10% of sequence in fragments shorter than     691 bp
  15% of sequence in fragments shorter than     921 bp
  20% of sequence in fragments shorter than    1142 bp
  25% of sequence in fragments shorter than    1362 bp
  30% of sequence in fragments shorter than    1578 bp
  35% of sequence in fragments shorter than    1801 bp
  40% of sequence in fragments shorter than    2024 bp
  45% of sequence in fragments shorter than    2259 bp
  50% of sequence in fragments shorter than    2506 bp
  55% of sequence in fragments shorter than    2775 bp
  60% of sequence in fragments shorter than    3060 bp
  65% of sequence in fragments shorter than    3375 bp
  70% of sequence in fragments shorter than    3736 bp
  75% of sequence in fragments shorter than    4148 bp
  80% of sequence in fragments shorter than    4647 bp
  85% of sequence in fragments shorter than    5286 bp
  90% of sequence in fragments shorter than    6178 bp
  95% of sequence in fragments shorter than    7670 bp

# EsaBC3I.SacI (TCGA+GAGCTC) double digest, 2206 thousand fragments (1603346+603323 sites), 1350 bp average fragment length
   5% of sequence in fragments shorter than     463 bp
  10% of sequence in fragments shorter than     710 bp
  15% of sequence in fragments shorter than     923 bp
  20% of sequence in fragments shorter than    1130 bp
  25% of sequence in fragments shorter than    1333 bp
  30% of sequence in fragments shorter than    1529 bp
  35% of sequence in fragments shorter than    1741 bp
  40% of sequence in fragments shorter than    1961 bp
  45% of sequence in fragments shorter than    2199 bp
  50% of sequence in fragments shorter than    2449 bp
  55% of sequence in fragments shorter than    2719 bp
  60% of sequence in fragments shorter than    3012 bp
  65% of sequence in fragments shorter than    3341 bp
  70% of sequence in fragments shorter than    3715 bp
  75% of sequence in fragments shorter than    4150 bp
  80% of sequence in fragments shorter than    4680 bp
  85% of sequence in fragments shorter than    5359 bp
  90% of sequence in fragments shorter than    6322 bp
  95% of sequence in fragments shorter than    8004 bp

# EcoRV.HhaI (GATATC+GCGC) double digest, 2202 thousand fragments (446473+1756502 sites), 1353 bp average fragment length
   5% of sequence in fragments shorter than     463 bp
  10% of sequence in fragments shorter than     776 bp
  15% of sequence in fragments shorter than    1074 bp
  20% of sequence in fragments shorter than    1367 bp
  25% of sequence in fragments shorter than    1659 bp
  30% of sequence in fragments shorter than    1948 bp
  35% of sequence in fragments shorter than    2249 bp
  40% of sequence in fragments shorter than    2567 bp
  45% of sequence in fragments shorter than    2896 bp
  50% of sequence in fragments shorter than    3252 bp
  55% of sequence in fragments shorter than    3642 bp
  60% of sequence in fragments shorter than    4070 bp
  65% of sequence in fragments shorter than    4544 bp
  70% of sequence in fragments shorter than    5089 bp
  75% of sequence in fragments shorter than    5719 bp
  80% of sequence in fragments shorter than    6480 bp
  85% of sequence in fragments shorter than    7450 bp
  90% of sequence in fragments shorter than    8801 bp
  95% of sequence in fragments shorter than   11126 bp

# ApaLI.BfiI (GTGCAC+ACTGGG) double digest, 2202 thousand fragments (496255+1706594 sites), 1353 bp average fragment length
   5% of sequence in fragments shorter than     456 bp
  10% of sequence in fragments shorter than     703 bp
  15% of sequence in fragments shorter than     926 bp
  20% of sequence in fragments shorter than    1133 bp
  25% of sequence in fragments shorter than    1343 bp
  30% of sequence in fragments shorter than    1554 bp
  35% of sequence in fragments shorter than    1767 bp
  40% of sequence in fragments shorter than    1990 bp
  45% of sequence in fragments shorter than    2209 bp
  50% of sequence in fragments shorter than    2456 bp
  55% of sequence in fragments shorter than    2728 bp
  60% of sequence in fragments shorter than    2995 bp
  65% of sequence in fragments shorter than    3317 bp
  70% of sequence in fragments shorter than    3688 bp
  75% of sequence in fragments shorter than    4131 bp
  80% of sequence in fragments shorter than    4666 bp
  85% of sequence in fragments shorter than    5338 bp
  90% of sequence in fragments shorter than    6334 bp
  95% of sequence in fragments shorter than    8234 bp

# FauI.NcoI (CCCGC+CCATGG) double digest, 2198 thousand fragments (1439772+759594 sites), 1355 bp average fragment length
   5% of sequence in fragments shorter than     456 bp
  10% of sequence in fragments shorter than     772 bp
  15% of sequence in fragments shorter than    1059 bp
  20% of sequence in fragments shorter than    1332 bp
  25% of sequence in fragments shorter than    1602 bp
  30% of sequence in fragments shorter than    1896 bp
  35% of sequence in fragments shorter than    2193 bp
  40% of sequence in fragments shorter than    2516 bp
  45% of sequence in fragments shorter than    2854 bp
  50% of sequence in fragments shorter than    3222 bp
  55% of sequence in fragments shorter than    3617 bp
  60% of sequence in fragments shorter than    4062 bp
  65% of sequence in fragments shorter than    4529 bp
  70% of sequence in fragments shorter than    5088 bp
  75% of sequence in fragments shorter than    5718 bp
  80% of sequence in fragments shorter than    6517 bp
  85% of sequence in fragments shorter than    7575 bp
  90% of sequence in fragments shorter than    9087 bp
  95% of sequence in fragments shorter than   11923 bp

# AflIII.BsaAI (ACRYGT+YACGTR) double digest, 2198 thousand fragments (1485394+713801 sites), 1355 bp average fragment length
   5% of sequence in fragments shorter than     542 bp
  10% of sequence in fragments shorter than     843 bp
  15% of sequence in fragments shorter than    1089 bp
  20% of sequence in fragments shorter than    1328 bp
  25% of sequence in fragments shorter than    1559 bp
  30% of sequence in fragments shorter than    1788 bp
  35% of sequence in fragments shorter than    2022 bp
  40% of sequence in fragments shorter than    2262 bp
  45% of sequence in fragments shorter than    2518 bp
  50% of sequence in fragments shorter than    2781 bp
  55% of sequence in fragments shorter than    3062 bp
  60% of sequence in fragments shorter than    3370 bp
  65% of sequence in fragments shorter than    3699 bp
  70% of sequence in fragments shorter than    4080 bp
  75% of sequence in fragments shorter than    4520 bp
  80% of sequence in fragments shorter than    5063 bp
  85% of sequence in fragments shorter than    5756 bp
  90% of sequence in fragments shorter than    6738 bp
  95% of sequence in fragments shorter than    8461 bp

# PsiI.SphI (TTATAA+GCATGC) double digest, 2198 thousand fragments (1647912+550952 sites), 1355 bp average fragment length
   5% of sequence in fragments shorter than     462 bp
  10% of sequence in fragments shorter than     710 bp
  15% of sequence in fragments shorter than     928 bp
  20% of sequence in fragments shorter than    1135 bp
  25% of sequence in fragments shorter than    1341 bp
  30% of sequence in fragments shorter than    1545 bp
  35% of sequence in fragments shorter than    1758 bp
  40% of sequence in fragments shorter than    1978 bp
  45% of sequence in fragments shorter than    2210 bp
  50% of sequence in fragments shorter than    2462 bp
  55% of sequence in fragments shorter than    2738 bp
  60% of sequence in fragments shorter than    3024 bp
  65% of sequence in fragments shorter than    3362 bp
  70% of sequence in fragments shorter than    3746 bp
  75% of sequence in fragments shorter than    4198 bp
  80% of sequence in fragments shorter than    4771 bp
  85% of sequence in fragments shorter than    5541 bp
  90% of sequence in fragments shorter than    6675 bp
  95% of sequence in fragments shorter than    8997 bp

# AvrII.EsaBC3I (CCTAGG+TCGA) double digest, 2197 thousand fragments (594956+1603346 sites), 1355 bp average fragment length
   5% of sequence in fragments shorter than     470 bp
  10% of sequence in fragments shorter than     703 bp
  15% of sequence in fragments shorter than     918 bp
  20% of sequence in fragments shorter than    1122 bp
  25% of sequence in fragments shorter than    1322 bp
  30% of sequence in fragments shorter than    1515 bp
  35% of sequence in fragments shorter than    1721 bp
  40% of sequence in fragments shorter than    1933 bp
  45% of sequence in fragments shorter than    2159 bp
  50% of sequence in fragments shorter than    2396 bp
  55% of sequence in fragments shorter than    2651 bp
  60% of sequence in fragments shorter than    2928 bp
  65% of sequence in fragments shorter than    3234 bp
  70% of sequence in fragments shorter than    3589 bp
  75% of sequence in fragments shorter than    4000 bp
  80% of sequence in fragments shorter than    4504 bp
  85% of sequence in fragments shorter than    5145 bp
  90% of sequence in fragments shorter than    6055 bp
  95% of sequence in fragments shorter than    7642 bp

# BspMI.NcoI (ACCTGC+CCATGG) double digest, 2197 thousand fragments (1438129+759594 sites), 1356 bp average fragment length
   5% of sequence in fragments shorter than     440 bp
  10% of sequence in fragments shorter than     703 bp
  15% of sequence in fragments shorter than     931 bp
  20% of sequence in fragments shorter than    1154 bp
  25% of sequence in fragments shorter than    1371 bp
  30% of sequence in fragments shorter than    1589 bp
  35% of sequence in fragments shorter than    1817 bp
  40% of sequence in fragments shorter than    2041 bp
  45% of sequence in fragments shorter than    2291 bp
  50% of sequence in fragments shorter than    2560 bp
  55% of sequence in fragments shorter than    2844 bp
  60% of sequence in fragments shorter than    3123 bp
  65% of sequence in fragments shorter than    3467 bp
  70% of sequence in fragments shorter than    3865 bp
  75% of sequence in fragments shorter than    4326 bp
  80% of sequence in fragments shorter than    4887 bp
  85% of sequence in fragments shorter than    5591 bp
  90% of sequence in fragments shorter than    6607 bp
  95% of sequence in fragments shorter than    8391 bp

# AflII.HgiCI (CTTAAG+GGYRCC) double digest, 2193 thousand fragments (643284+1550877 sites), 1358 bp average fragment length
   5% of sequence in fragments shorter than     461 bp
  10% of sequence in fragments shorter than     696 bp
  15% of sequence in fragments shorter than     907 bp
  20% of sequence in fragments shorter than    1118 bp
  25% of sequence in fragments shorter than    1315 bp
  30% of sequence in fragments shorter than    1520 bp
  35% of sequence in fragments shorter than    1721 bp
  40% of sequence in fragments shorter than    1939 bp
  45% of sequence in fragments shorter than    2169 bp
  50% of sequence in fragments shorter than    2413 bp
  55% of sequence in fragments shorter than    2679 bp
  60% of sequence in fragments shorter than    2959 bp
  65% of sequence in fragments shorter than    3277 bp
  70% of sequence in fragments shorter than    3648 bp
  75% of sequence in fragments shorter than    4081 bp
  80% of sequence in fragments shorter than    4610 bp
  85% of sequence in fragments shorter than    5318 bp
  90% of sequence in fragments shorter than    6354 bp
  95% of sequence in fragments shorter than    8538 bp

# AflII.BsmI (CTTAAG+GAATGC) double digest, 2192 thousand fragments (643284+1549350 sites), 1359 bp average fragment length
   5% of sequence in fragments shorter than     450 bp
  10% of sequence in fragments shorter than     770 bp
  15% of sequence in fragments shorter than    1028 bp
  20% of sequence in fragments shorter than    1265 bp
  25% of sequence in fragments shorter than    1500 bp
  30% of sequence in fragments shorter than    1728 bp
  35% of sequence in fragments shorter than    1959 bp
  40% of sequence in fragments shorter than    2196 bp
  45% of sequence in fragments shorter than    2439 bp
  50% of sequence in fragments shorter than    2692 bp
  55% of sequence in fragments shorter than    2961 bp
  60% of sequence in fragments shorter than    3257 bp
  65% of sequence in fragments shorter than    3588 bp
  70% of sequence in fragments shorter than    3962 bp
  75% of sequence in fragments shorter than    4393 bp
  80% of sequence in fragments shorter than    4913 bp
  85% of sequence in fragments shorter than    5574 bp
  90% of sequence in fragments shorter than    6486 bp
  95% of sequence in fragments shorter than    8045 bp

# PsiI.ScaI (TTATAA+AGTACT) double digest, 2191 thousand fragments (1647912+543793 sites), 1359 bp average fragment length
   5% of sequence in fragments shorter than     455 bp
  10% of sequence in fragments shorter than     705 bp
  15% of sequence in fragments shorter than     928 bp
  20% of sequence in fragments shorter than    1141 bp
  25% of sequence in fragments shorter than    1353 bp
  30% of sequence in fragments shorter than    1570 bp
  35% of sequence in fragments shorter than    1794 bp
  40% of sequence in fragments shorter than    2030 bp
  45% of sequence in fragments shorter than    2287 bp
  50% of sequence in fragments shorter than    2566 bp
  55% of sequence in fragments shorter than    2873 bp
  60% of sequence in fragments shorter than    3195 bp
  65% of sequence in fragments shorter than    3584 bp
  70% of sequence in fragments shorter than    4050 bp
  75% of sequence in fragments shorter than    4620 bp
  80% of sequence in fragments shorter than    5380 bp
  85% of sequence in fragments shorter than    6446 bp
  90% of sequence in fragments shorter than    8175 bp
  95% of sequence in fragments shorter than   12408 bp

# AflIII.BceAI (ACRYGT+ACGGC) double digest, 2190 thousand fragments (1485394+705178 sites), 1360 bp average fragment length
   5% of sequence in fragments shorter than     470 bp
  10% of sequence in fragments shorter than     741 bp
  15% of sequence in fragments shorter than     975 bp
  20% of sequence in fragments shorter than    1194 bp
  25% of sequence in fragments shorter than    1409 bp
  30% of sequence in fragments shorter than    1627 bp
  35% of sequence in fragments shorter than    1847 bp
  40% of sequence in fragments shorter than    2070 bp
  45% of sequence in fragments shorter than    2303 bp
  50% of sequence in fragments shorter than    2555 bp
  55% of sequence in fragments shorter than    2819 bp
  60% of sequence in fragments shorter than    3110 bp
  65% of sequence in fragments shorter than    3439 bp
  70% of sequence in fragments shorter than    3808 bp
  75% of sequence in fragments shorter than    4238 bp
  80% of sequence in fragments shorter than    4749 bp
  85% of sequence in fragments shorter than    5367 bp
  90% of sequence in fragments shorter than    6259 bp
  95% of sequence in fragments shorter than    7883 bp

# AccI.PpuMI (GTMKAC+RGGWCCY) double digest, 2185 thousand fragments (1101063+1085138 sites), 1363 bp average fragment length
   5% of sequence in fragments shorter than     467 bp
  10% of sequence in fragments shorter than     723 bp
  15% of sequence in fragments shorter than     948 bp
  20% of sequence in fragments shorter than    1158 bp
  25% of sequence in fragments shorter than    1360 bp
  30% of sequence in fragments shorter than    1561 bp
  35% of sequence in fragments shorter than    1750 bp
  40% of sequence in fragments shorter than    1962 bp
  45% of sequence in fragments shorter than    2188 bp
  50% of sequence in fragments shorter than    2429 bp
  55% of sequence in fragments shorter than    2686 bp
  60% of sequence in fragments shorter than    2965 bp
  65% of sequence in fragments shorter than    3272 bp
  70% of sequence in fragments shorter than    3597 bp
  75% of sequence in fragments shorter than    3995 bp
  80% of sequence in fragments shorter than    4491 bp
  85% of sequence in fragments shorter than    5125 bp
  90% of sequence in fragments shorter than    6017 bp
  95% of sequence in fragments shorter than    7693 bp

# AccI.PvuII (GTMKAC+CAGCTG) double digest, 2184 thousand fragments (1101063+1084260 sites), 1363 bp average fragment length
   5% of sequence in fragments shorter than     475 bp
  10% of sequence in fragments shorter than     725 bp
  15% of sequence in fragments shorter than     942 bp
  20% of sequence in fragments shorter than    1145 bp
  25% of sequence in fragments shorter than    1341 bp
  30% of sequence in fragments shorter than    1533 bp
  35% of sequence in fragments shorter than    1734 bp
  40% of sequence in fragments shorter than    1938 bp
  45% of sequence in fragments shorter than    2159 bp
  50% of sequence in fragments shorter than    2388 bp
  55% of sequence in fragments shorter than    2627 bp
  60% of sequence in fragments shorter than    2893 bp
  65% of sequence in fragments shorter than    3188 bp
  70% of sequence in fragments shorter than    3522 bp
  75% of sequence in fragments shorter than    3913 bp
  80% of sequence in fragments shorter than    4374 bp
  85% of sequence in fragments shorter than    4978 bp
  90% of sequence in fragments shorter than    5749 bp
  95% of sequence in fragments shorter than    7264 bp

# HindIII.PstI (AAGCTT+CTGCAG) double digest, 2181 thousand fragments (860365+1321469 sites), 1366 bp average fragment length
   5% of sequence in fragments shorter than     467 bp
  10% of sequence in fragments shorter than     712 bp
  15% of sequence in fragments shorter than     936 bp
  20% of sequence in fragments shorter than    1144 bp
  25% of sequence in fragments shorter than    1350 bp
  30% of sequence in fragments shorter than    1552 bp
  35% of sequence in fragments shorter than    1764 bp
  40% of sequence in fragments shorter than    1963 bp
  45% of sequence in fragments shorter than    2187 bp
  50% of sequence in fragments shorter than    2428 bp
  55% of sequence in fragments shorter than    2687 bp
  60% of sequence in fragments shorter than    2970 bp
  65% of sequence in fragments shorter than    3284 bp
  70% of sequence in fragments shorter than    3638 bp
  75% of sequence in fragments shorter than    4050 bp
  80% of sequence in fragments shorter than    4545 bp
  85% of sequence in fragments shorter than    5179 bp
  90% of sequence in fragments shorter than    6081 bp
  95% of sequence in fragments shorter than    7703 bp

# AflIII.BciVI (ACRYGT+GTATCC) double digest, 2181 thousand fragments (1485394+696093 sites), 1366 bp average fragment length
   5% of sequence in fragments shorter than     487 bp
  10% of sequence in fragments shorter than     757 bp
  15% of sequence in fragments shorter than     986 bp
  20% of sequence in fragments shorter than    1200 bp
  25% of sequence in fragments shorter than    1399 bp
  30% of sequence in fragments shorter than    1602 bp
  35% of sequence in fragments shorter than    1804 bp
  40% of sequence in fragments shorter than    2018 bp
  45% of sequence in fragments shorter than    2243 bp
  50% of sequence in fragments shorter than    2485 bp
  55% of sequence in fragments shorter than    2744 bp
  60% of sequence in fragments shorter than    3019 bp
  65% of sequence in fragments shorter than    3328 bp
  70% of sequence in fragments shorter than    3678 bp
  75% of sequence in fragments shorter than    4093 bp
  80% of sequence in fragments shorter than    4583 bp
  85% of sequence in fragments shorter than    5213 bp
  90% of sequence in fragments shorter than    6110 bp
  95% of sequence in fragments shorter than    7741 bp

# BsmI.HaeII (GAATGC+RGCGCY) double digest, 2178 thousand fragments (1549350+630101 sites), 1367 bp average fragment length
   5% of sequence in fragments shorter than     442 bp
  10% of sequence in fragments shorter than     784 bp
  15% of sequence in fragments shorter than    1071 bp
  20% of sequence in fragments shorter than    1337 bp
  25% of sequence in fragments shorter than    1599 bp
  30% of sequence in fragments shorter than    1857 bp
  35% of sequence in fragments shorter than    2113 bp
  40% of sequence in fragments shorter than    2380 bp
  45% of sequence in fragments shorter than    2651 bp
  50% of sequence in fragments shorter than    2936 bp
  55% of sequence in fragments shorter than    3245 bp
  60% of sequence in fragments shorter than    3583 bp
  65% of sequence in fragments shorter than    3955 bp
  70% of sequence in fragments shorter than    4374 bp
  75% of sequence in fragments shorter than    4854 bp
  80% of sequence in fragments shorter than    5438 bp
  85% of sequence in fragments shorter than    6164 bp
  90% of sequence in fragments shorter than    7175 bp
  95% of sequence in fragments shorter than    8868 bp

# BclI.FauI (TGATCA+CCCGC) double digest, 2178 thousand fragments (738785+1439772 sites), 1368 bp average fragment length
   5% of sequence in fragments shorter than     482 bp
  10% of sequence in fragments shorter than     788 bp
  15% of sequence in fragments shorter than    1049 bp
  20% of sequence in fragments shorter than    1313 bp
  25% of sequence in fragments shorter than    1565 bp
  30% of sequence in fragments shorter than    1826 bp
  35% of sequence in fragments shorter than    2088 bp
  40% of sequence in fragments shorter than    2370 bp
  45% of sequence in fragments shorter than    2658 bp
  50% of sequence in fragments shorter than    2977 bp
  55% of sequence in fragments shorter than    3307 bp
  60% of sequence in fragments shorter than    3683 bp
  65% of sequence in fragments shorter than    4104 bp
  70% of sequence in fragments shorter than    4583 bp
  75% of sequence in fragments shorter than    5145 bp
  80% of sequence in fragments shorter than    5830 bp
  85% of sequence in fragments shorter than    6734 bp
  90% of sequence in fragments shorter than    8027 bp
  95% of sequence in fragments shorter than   10471 bp

# BclI.BspMI (TGATCA+ACCTGC) double digest, 2176 thousand fragments (738785+1438129 sites), 1369 bp average fragment length
   5% of sequence in fragments shorter than     474 bp
  10% of sequence in fragments shorter than     721 bp
  15% of sequence in fragments shorter than     933 bp
  20% of sequence in fragments shorter than    1134 bp
  25% of sequence in fragments shorter than    1337 bp
  30% of sequence in fragments shorter than    1530 bp
  35% of sequence in fragments shorter than    1731 bp
  40% of sequence in fragments shorter than    1944 bp
  45% of sequence in fragments shorter than    2153 bp
  50% of sequence in fragments shorter than    2363 bp
  55% of sequence in fragments shorter than    2612 bp
  60% of sequence in fragments shorter than    2877 bp
  65% of sequence in fragments shorter than    3177 bp
  70% of sequence in fragments shorter than    3510 bp
  75% of sequence in fragments shorter than    3907 bp
  80% of sequence in fragments shorter than    4378 bp
  85% of sequence in fragments shorter than    4983 bp
  90% of sequence in fragments shorter than    5833 bp
  95% of sequence in fragments shorter than    7308 bp

# EsaBC3I.HgaI (TCGA+GACGC) double digest, 2174 thousand fragments (1603346+571889 sites), 1370 bp average fragment length
   5% of sequence in fragments shorter than     454 bp
  10% of sequence in fragments shorter than     717 bp
  15% of sequence in fragments shorter than     948 bp
  20% of sequence in fragments shorter than    1173 bp
  25% of sequence in fragments shorter than    1387 bp
  30% of sequence in fragments shorter than    1618 bp
  35% of sequence in fragments shorter than    1853 bp
  40% of sequence in fragments shorter than    2099 bp
  45% of sequence in fragments shorter than    2364 bp
  50% of sequence in fragments shorter than    2646 bp
  55% of sequence in fragments shorter than    2950 bp
  60% of sequence in fragments shorter than    3282 bp
  65% of sequence in fragments shorter than    3656 bp
  70% of sequence in fragments shorter than    4083 bp
  75% of sequence in fragments shorter than    4588 bp
  80% of sequence in fragments shorter than    5195 bp
  85% of sequence in fragments shorter than    5984 bp
  90% of sequence in fragments shorter than    7095 bp
  95% of sequence in fragments shorter than    8992 bp

# EciI.EsaBC3I (GGCGGA+TCGA) double digest, 2174 thousand fragments (571273+1603346 sites), 1370 bp average fragment length
   5% of sequence in fragments shorter than     461 bp
  10% of sequence in fragments shorter than     738 bp
  15% of sequence in fragments shorter than     981 bp
  20% of sequence in fragments shorter than    1215 bp
  25% of sequence in fragments shorter than    1440 bp
  30% of sequence in fragments shorter than    1682 bp
  35% of sequence in fragments shorter than    1927 bp
  40% of sequence in fragments shorter than    2188 bp
  45% of sequence in fragments shorter than    2465 bp
  50% of sequence in fragments shorter than    2760 bp
  55% of sequence in fragments shorter than    3081 bp
  60% of sequence in fragments shorter than    3431 bp
  65% of sequence in fragments shorter than    3826 bp
  70% of sequence in fragments shorter than    4279 bp
  75% of sequence in fragments shorter than    4813 bp
  80% of sequence in fragments shorter than    5465 bp
  85% of sequence in fragments shorter than    6292 bp
  90% of sequence in fragments shorter than    7474 bp
  95% of sequence in fragments shorter than    9506 bp

# FauI.FnuDII (CCCGC+CGCG) double digest, 2173 thousand fragments (1439772+733940 sites), 1371 bp average fragment length
   5% of sequence in fragments shorter than     522 bp
  10% of sequence in fragments shorter than     969 bp
  15% of sequence in fragments shorter than    1388 bp
  20% of sequence in fragments shorter than    1830 bp
  25% of sequence in fragments shorter than    2282 bp
  30% of sequence in fragments shorter than    2770 bp
  35% of sequence in fragments shorter than    3291 bp
  40% of sequence in fragments shorter than    3866 bp
  45% of sequence in fragments shorter than    4454 bp
  50% of sequence in fragments shorter than    5140 bp
  55% of sequence in fragments shorter than    5900 bp
  60% of sequence in fragments shorter than    6754 bp
  65% of sequence in fragments shorter than    7725 bp
  70% of sequence in fragments shorter than    8853 bp
  75% of sequence in fragments shorter than   10205 bp
  80% of sequence in fragments shorter than   11833 bp
  85% of sequence in fragments shorter than   14006 bp
  90% of sequence in fragments shorter than   17223 bp
  95% of sequence in fragments shorter than   23267 bp

# PstI.XbaI (CTGCAG+TCTAGA) double digest, 2172 thousand fragments (1321469+850998 sites), 1372 bp average fragment length
   5% of sequence in fragments shorter than     476 bp
  10% of sequence in fragments shorter than     719 bp
  15% of sequence in fragments shorter than     931 bp
  20% of sequence in fragments shorter than    1138 bp
  25% of sequence in fragments shorter than    1346 bp
  30% of sequence in fragments shorter than    1536 bp
  35% of sequence in fragments shorter than    1742 bp
  40% of sequence in fragments shorter than    1944 bp
  45% of sequence in fragments shorter than    2164 bp
  50% of sequence in fragments shorter than    2394 bp
  55% of sequence in fragments shorter than    2641 bp
  60% of sequence in fragments shorter than    2917 bp
  65% of sequence in fragments shorter than    3220 bp
  70% of sequence in fragments shorter than    3566 bp
  75% of sequence in fragments shorter than    3970 bp
  80% of sequence in fragments shorter than    4456 bp
  85% of sequence in fragments shorter than    5067 bp
  90% of sequence in fragments shorter than    5929 bp
  95% of sequence in fragments shorter than    7425 bp

# BspMI.FnuDII (ACCTGC+CGCG) double digest, 2171 thousand fragments (1438129+733940 sites), 1372 bp average fragment length
   5% of sequence in fragments shorter than     472 bp
  10% of sequence in fragments shorter than     781 bp
  15% of sequence in fragments shorter than    1049 bp
  20% of sequence in fragments shorter than    1321 bp
  25% of sequence in fragments shorter than    1582 bp
  30% of sequence in fragments shorter than    1856 bp
  35% of sequence in fragments shorter than    2114 bp
  40% of sequence in fragments shorter than    2407 bp
  45% of sequence in fragments shorter than    2720 bp
  50% of sequence in fragments shorter than    3049 bp
  55% of sequence in fragments shorter than    3363 bp
  60% of sequence in fragments shorter than    3758 bp
  65% of sequence in fragments shorter than    4201 bp
  70% of sequence in fragments shorter than    4709 bp
  75% of sequence in fragments shorter than    5289 bp
  80% of sequence in fragments shorter than    6008 bp
  85% of sequence in fragments shorter than    6930 bp
  90% of sequence in fragments shorter than    8236 bp
  95% of sequence in fragments shorter than   10503 bp

# PpuMI.PvuII (RGGWCCY+CAGCTG) double digest, 2168 thousand fragments (1085138+1084260 sites), 1373 bp average fragment length
   5% of sequence in fragments shorter than     441 bp
  10% of sequence in fragments shorter than     715 bp
  15% of sequence in fragments shorter than     961 bp
  20% of sequence in fragments shorter than    1205 bp
  25% of sequence in fragments shorter than    1449 bp
  30% of sequence in fragments shorter than    1702 bp
  35% of sequence in fragments shorter than    1963 bp
  40% of sequence in fragments shorter than    2239 bp
  45% of sequence in fragments shorter than    2533 bp
  50% of sequence in fragments shorter than    2836 bp
  55% of sequence in fragments shorter than    3175 bp
  60% of sequence in fragments shorter than    3551 bp
  65% of sequence in fragments shorter than    3949 bp
  70% of sequence in fragments shorter than    4442 bp
  75% of sequence in fragments shorter than    5023 bp
  80% of sequence in fragments shorter than    5731 bp
  85% of sequence in fragments shorter than    6672 bp
  90% of sequence in fragments shorter than    8053 bp
  95% of sequence in fragments shorter than   10767 bp

# EcoRI.PstI (GAATTC+CTGCAG) double digest, 2168 thousand fragments (847401+1321469 sites), 1374 bp average fragment length
   5% of sequence in fragments shorter than     463 bp
  10% of sequence in fragments shorter than     709 bp
  15% of sequence in fragments shorter than     932 bp
  20% of sequence in fragments shorter than    1147 bp
  25% of sequence in fragments shorter than    1358 bp
  30% of sequence in fragments shorter than    1565 bp
  35% of sequence in fragments shorter than    1781 bp
  40% of sequence in fragments shorter than    2001 bp
  45% of sequence in fragments shorter than    2222 bp
  50% of sequence in fragments shorter than    2471 bp
  55% of sequence in fragments shorter than    2730 bp
  60% of sequence in fragments shorter than    3016 bp
  65% of sequence in fragments shorter than    3335 bp
  70% of sequence in fragments shorter than    3692 bp
  75% of sequence in fragments shorter than    4107 bp
  80% of sequence in fragments shorter than    4613 bp
  85% of sequence in fragments shorter than    5249 bp
  90% of sequence in fragments shorter than    6141 bp
  95% of sequence in fragments shorter than    7665 bp

# EsaBC3I.MfeI (TCGA+CAATTG) double digest, 2167 thousand fragments (1603346+564303 sites), 1375 bp average fragment length
   5% of sequence in fragments shorter than     479 bp
  10% of sequence in fragments shorter than     723 bp
  15% of sequence in fragments shorter than     936 bp
  20% of sequence in fragments shorter than    1135 bp
  25% of sequence in fragments shorter than    1335 bp
  30% of sequence in fragments shorter than    1527 bp
  35% of sequence in fragments shorter than    1731 bp
  40% of sequence in fragments shorter than    1938 bp
  45% of sequence in fragments shorter than    2155 bp
  50% of sequence in fragments shorter than    2382 bp
  55% of sequence in fragments shorter than    2630 bp
  60% of sequence in fragments shorter than    2898 bp
  65% of sequence in fragments shorter than    3190 bp
  70% of sequence in fragments shorter than    3525 bp
  75% of sequence in fragments shorter than    3914 bp
  80% of sequence in fragments shorter than    4383 bp
  85% of sequence in fragments shorter than    4971 bp
  90% of sequence in fragments shorter than    5789 bp
  95% of sequence in fragments shorter than    7181 bp

# ApaI.BfiI (GGGCCC+ACTGGG) double digest, 2166 thousand fragments (460339+1706594 sites), 1375 bp average fragment length
   5% of sequence in fragments shorter than     450 bp
  10% of sequence in fragments shorter than     717 bp
  15% of sequence in fragments shorter than     957 bp
  20% of sequence in fragments shorter than    1186 bp
  25% of sequence in fragments shorter than    1418 bp
  30% of sequence in fragments shorter than    1655 bp
  35% of sequence in fragments shorter than    1894 bp
  40% of sequence in fragments shorter than    2138 bp
  45% of sequence in fragments shorter than    2395 bp
  50% of sequence in fragments shorter than    2682 bp
  55% of sequence in fragments shorter than    2963 bp
  60% of sequence in fragments shorter than    3302 bp
  65% of sequence in fragments shorter than    3687 bp
  70% of sequence in fragments shorter than    4126 bp
  75% of sequence in fragments shorter than    4635 bp
  80% of sequence in fragments shorter than    5243 bp
  85% of sequence in fragments shorter than    6053 bp
  90% of sequence in fragments shorter than    7244 bp
  95% of sequence in fragments shorter than    9590 bp

# AvaIII.BscGI (ATGCAT+CCCGT) double digest, 2163 thousand fragments (933357+1231070 sites), 1377 bp average fragment length
   5% of sequence in fragments shorter than     430 bp
  10% of sequence in fragments shorter than     696 bp
  15% of sequence in fragments shorter than     937 bp
  20% of sequence in fragments shorter than    1163 bp
  25% of sequence in fragments shorter than    1382 bp
  30% of sequence in fragments shorter than    1605 bp
  35% of sequence in fragments shorter than    1833 bp
  40% of sequence in fragments shorter than    2069 bp
  45% of sequence in fragments shorter than    2315 bp
  50% of sequence in fragments shorter than    2579 bp
  55% of sequence in fragments shorter than    2860 bp
  60% of sequence in fragments shorter than    3165 bp
  65% of sequence in fragments shorter than    3500 bp
  70% of sequence in fragments shorter than    3884 bp
  75% of sequence in fragments shorter than    4329 bp
  80% of sequence in fragments shorter than    4859 bp
  85% of sequence in fragments shorter than    5532 bp
  90% of sequence in fragments shorter than    6456 bp
  95% of sequence in fragments shorter than    8021 bp

# BscGI.StuI (CCCGT+AGGCCT) double digest, 2156 thousand fragments (1231070+925729 sites), 1381 bp average fragment length
   5% of sequence in fragments shorter than     397 bp
  10% of sequence in fragments shorter than     696 bp
  15% of sequence in fragments shorter than     968 bp
  20% of sequence in fragments shorter than    1233 bp
  25% of sequence in fragments shorter than    1500 bp
  30% of sequence in fragments shorter than    1779 bp
  35% of sequence in fragments shorter than    2049 bp
  40% of sequence in fragments shorter than    2352 bp
  45% of sequence in fragments shorter than    2675 bp
  50% of sequence in fragments shorter than    3021 bp
  55% of sequence in fragments shorter than    3399 bp
  60% of sequence in fragments shorter than    3809 bp
  65% of sequence in fragments shorter than    4274 bp
  70% of sequence in fragments shorter than    4798 bp
  75% of sequence in fragments shorter than    5421 bp
  80% of sequence in fragments shorter than    6179 bp
  85% of sequence in fragments shorter than    7145 bp
  90% of sequence in fragments shorter than    8479 bp
  95% of sequence in fragments shorter than   10804 bp

# HhaI.SpeI (GCGC+ACTAGT) double digest, 2156 thousand fragments (1756502+400286 sites), 1381 bp average fragment length
   5% of sequence in fragments shorter than     475 bp
  10% of sequence in fragments shorter than     793 bp
  15% of sequence in fragments shorter than    1097 bp
  20% of sequence in fragments shorter than    1398 bp
  25% of sequence in fragments shorter than    1701 bp
  30% of sequence in fragments shorter than    1999 bp
  35% of sequence in fragments shorter than    2318 bp
  40% of sequence in fragments shorter than    2651 bp
  45% of sequence in fragments shorter than    3003 bp
  50% of sequence in fragments shorter than    3385 bp
  55% of sequence in fragments shorter than    3783 bp
  60% of sequence in fragments shorter than    4236 bp
  65% of sequence in fragments shorter than    4727 bp
  70% of sequence in fragments shorter than    5280 bp
  75% of sequence in fragments shorter than    5938 bp
  80% of sequence in fragments shorter than    6750 bp
  85% of sequence in fragments shorter than    7785 bp
  90% of sequence in fragments shorter than    9220 bp
  95% of sequence in fragments shorter than   11810 bp

# EsaBC3I.SphI (TCGA+GCATGC) double digest, 2153 thousand fragments (1603346+550952 sites), 1383 bp average fragment length
   5% of sequence in fragments shorter than     477 bp
  10% of sequence in fragments shorter than     729 bp
  15% of sequence in fragments shorter than     950 bp
  20% of sequence in fragments shorter than    1160 bp
  25% of sequence in fragments shorter than    1367 bp
  30% of sequence in fragments shorter than    1567 bp
  35% of sequence in fragments shorter than    1779 bp
  40% of sequence in fragments shorter than    2003 bp
  45% of sequence in fragments shorter than    2238 bp
  50% of sequence in fragments shorter than    2489 bp
  55% of sequence in fragments shorter than    2758 bp
  60% of sequence in fragments shorter than    3050 bp
  65% of sequence in fragments shorter than    3378 bp
  70% of sequence in fragments shorter than    3748 bp
  75% of sequence in fragments shorter than    4183 bp
  80% of sequence in fragments shorter than    4713 bp
  85% of sequence in fragments shorter than    5391 bp
  90% of sequence in fragments shorter than    6343 bp
  95% of sequence in fragments shorter than    7988 bp

# HgiCI.SacI (GGYRCC+GAGCTC) double digest, 2153 thousand fragments (1550877+603323 sites), 1383 bp average fragment length
   5% of sequence in fragments shorter than     454 bp
  10% of sequence in fragments shorter than     710 bp
  15% of sequence in fragments shorter than     945 bp
  20% of sequence in fragments shorter than    1168 bp
  25% of sequence in fragments shorter than    1371 bp
  30% of sequence in fragments shorter than    1591 bp
  35% of sequence in fragments shorter than    1821 bp
  40% of sequence in fragments shorter than    2073 bp
  45% of sequence in fragments shorter than    2336 bp
  50% of sequence in fragments shorter than    2625 bp
  55% of sequence in fragments shorter than    2930 bp
  60% of sequence in fragments shorter than    3275 bp
  65% of sequence in fragments shorter than    3663 bp
  70% of sequence in fragments shorter than    4115 bp
  75% of sequence in fragments shorter than    4644 bp
  80% of sequence in fragments shorter than    5305 bp
  85% of sequence in fragments shorter than    6172 bp
  90% of sequence in fragments shorter than    7459 bp
  95% of sequence in fragments shorter than   10016 bp

# BsaAI.FauI (YACGTR+CCCGC) double digest, 2153 thousand fragments (713801+1439772 sites), 1384 bp average fragment length
   5% of sequence in fragments shorter than     474 bp
  10% of sequence in fragments shorter than     797 bp
  15% of sequence in fragments shorter than    1075 bp
  20% of sequence in fragments shorter than    1367 bp
  25% of sequence in fragments shorter than    1644 bp
  30% of sequence in fragments shorter than    1937 bp
  35% of sequence in fragments shorter than    2248 bp
  40% of sequence in fragments shorter than    2569 bp
  45% of sequence in fragments shorter than    2913 bp
  50% of sequence in fragments shorter than    3280 bp
  55% of sequence in fragments shorter than    3678 bp
  60% of sequence in fragments shorter than    4129 bp
  65% of sequence in fragments shorter than    4622 bp
  70% of sequence in fragments shorter than    5199 bp
  75% of sequence in fragments shorter than    5873 bp
  80% of sequence in fragments shorter than    6705 bp
  85% of sequence in fragments shorter than    7789 bp
  90% of sequence in fragments shorter than    9295 bp
  95% of sequence in fragments shorter than   12010 bp

# BfiI.EcoRV (ACTGGG+GATATC) double digest, 2152 thousand fragments (1706594+446473 sites), 1384 bp average fragment length
   5% of sequence in fragments shorter than     468 bp
  10% of sequence in fragments shorter than     724 bp
  15% of sequence in fragments shorter than     947 bp
  20% of sequence in fragments shorter than    1152 bp
  25% of sequence in fragments shorter than    1359 bp
  30% of sequence in fragments shorter than    1564 bp
  35% of sequence in fragments shorter than    1770 bp
  40% of sequence in fragments shorter than    1979 bp
  45% of sequence in fragments shorter than    2193 bp
  50% of sequence in fragments shorter than    2409 bp
  55% of sequence in fragments shorter than    2660 bp
  60% of sequence in fragments shorter than    2936 bp
  65% of sequence in fragments shorter than    3245 bp
  70% of sequence in fragments shorter than    3594 bp
  75% of sequence in fragments shorter than    4004 bp
  80% of sequence in fragments shorter than    4491 bp
  85% of sequence in fragments shorter than    5119 bp
  90% of sequence in fragments shorter than    6013 bp
  95% of sequence in fragments shorter than    7657 bp

# BsmI.SacI (GAATGC+GAGCTC) double digest, 2152 thousand fragments (1549350+603323 sites), 1384 bp average fragment length
   5% of sequence in fragments shorter than     456 bp
  10% of sequence in fragments shorter than     779 bp
  15% of sequence in fragments shorter than    1039 bp
  20% of sequence in fragments shorter than    1284 bp
  25% of sequence in fragments shorter than    1523 bp
  30% of sequence in fragments shorter than    1761 bp
  35% of sequence in fragments shorter than    2000 bp
  40% of sequence in fragments shorter than    2229 bp
  45% of sequence in fragments shorter than    2477 bp
  50% of sequence in fragments shorter than    2742 bp
  55% of sequence in fragments shorter than    3029 bp
  60% of sequence in fragments shorter than    3336 bp
  65% of sequence in fragments shorter than    3676 bp
  70% of sequence in fragments shorter than    4061 bp
  75% of sequence in fragments shorter than    4504 bp
  80% of sequence in fragments shorter than    5035 bp
  85% of sequence in fragments shorter than    5708 bp
  90% of sequence in fragments shorter than    6636 bp
  95% of sequence in fragments shorter than    8207 bp

# BsaAI.BspMI (YACGTR+ACCTGC) double digest, 2151 thousand fragments (713801+1438129 sites), 1385 bp average fragment length
   5% of sequence in fragments shorter than     462 bp
  10% of sequence in fragments shorter than     723 bp
  15% of sequence in fragments shorter than     945 bp
  20% of sequence in fragments shorter than    1164 bp
  25% of sequence in fragments shorter than    1380 bp
  30% of sequence in fragments shorter than    1595 bp
  35% of sequence in fragments shorter than    1817 bp
  40% of sequence in fragments shorter than    2048 bp
  45% of sequence in fragments shorter than    2288 bp
  50% of sequence in fragments shorter than    2549 bp
  55% of sequence in fragments shorter than    2826 bp
  60% of sequence in fragments shorter than    3107 bp
  65% of sequence in fragments shorter than    3428 bp
  70% of sequence in fragments shorter than    3810 bp
  75% of sequence in fragments shorter than    4256 bp
  80% of sequence in fragments shorter than    4791 bp
  85% of sequence in fragments shorter than    5470 bp
  90% of sequence in fragments shorter than    6422 bp
  95% of sequence in fragments shorter than    8055 bp

# EsaBC3I.ScaI (TCGA+AGTACT) double digest, 2146 thousand fragments (1603346+543793 sites), 1388 bp average fragment length
   5% of sequence in fragments shorter than     481 bp
  10% of sequence in fragments shorter than     731 bp
  15% of sequence in fragments shorter than     948 bp
  20% of sequence in fragments shorter than    1154 bp
  25% of sequence in fragments shorter than    1356 bp
  30% of sequence in fragments shorter than    1549 bp
  35% of sequence in fragments shorter than    1755 bp
  40% of sequence in fragments shorter than    1967 bp
  45% of sequence in fragments shorter than    2191 bp
  50% of sequence in fragments shorter than    2433 bp
  55% of sequence in fragments shorter than    2688 bp
  60% of sequence in fragments shorter than    2963 bp
  65% of sequence in fragments shorter than    3270 bp
  70% of sequence in fragments shorter than    3618 bp
  75% of sequence in fragments shorter than    4025 bp
  80% of sequence in fragments shorter than    4515 bp
  85% of sequence in fragments shorter than    5130 bp
  90% of sequence in fragments shorter than    6000 bp
  95% of sequence in fragments shorter than    7515 bp

# AvrII.HgiCI (CCTAGG+GGYRCC) double digest, 2145 thousand fragments (594956+1550877 sites), 1388 bp average fragment length
   5% of sequence in fragments shorter than     452 bp
  10% of sequence in fragments shorter than     708 bp
  15% of sequence in fragments shorter than     934 bp
  20% of sequence in fragments shorter than    1164 bp
  25% of sequence in fragments shorter than    1378 bp
  30% of sequence in fragments shorter than    1595 bp
  35% of sequence in fragments shorter than    1831 bp
  40% of sequence in fragments shorter than    2078 bp
  45% of sequence in fragments shorter than    2338 bp
  50% of sequence in fragments shorter than    2618 bp
  55% of sequence in fragments shorter than    2914 bp
  60% of sequence in fragments shorter than    3247 bp
  65% of sequence in fragments shorter than    3627 bp
  70% of sequence in fragments shorter than    4059 bp
  75% of sequence in fragments shorter than    4569 bp
  80% of sequence in fragments shorter than    5215 bp
  85% of sequence in fragments shorter than    6074 bp
  90% of sequence in fragments shorter than    7372 bp
  95% of sequence in fragments shorter than   10039 bp

# HhaI.HpaI (GCGC+GTTAAC) double digest, 2145 thousand fragments (1756502+389418 sites), 1388 bp average fragment length
   5% of sequence in fragments shorter than     473 bp
  10% of sequence in fragments shorter than     799 bp
  15% of sequence in fragments shorter than    1104 bp
  20% of sequence in fragments shorter than    1408 bp
  25% of sequence in fragments shorter than    1715 bp
  30% of sequence in fragments shorter than    2018 bp
  35% of sequence in fragments shorter than    2339 bp
  40% of sequence in fragments shorter than    2680 bp
  45% of sequence in fragments shorter than    3046 bp
  50% of sequence in fragments shorter than    3444 bp
  55% of sequence in fragments shorter than    3866 bp
  60% of sequence in fragments shorter than    4329 bp
  65% of sequence in fragments shorter than    4860 bp
  70% of sequence in fragments shorter than    5460 bp
  75% of sequence in fragments shorter than    6161 bp
  80% of sequence in fragments shorter than    7016 bp
  85% of sequence in fragments shorter than    8117 bp
  90% of sequence in fragments shorter than    9679 bp
  95% of sequence in fragments shorter than   12326 bp

# BceAI.FauI (ACGGC+CCCGC) double digest, 2144 thousand fragments (705178+1439772 sites), 1389 bp average fragment length
   5% of sequence in fragments shorter than     458 bp
  10% of sequence in fragments shorter than     812 bp
  15% of sequence in fragments shorter than    1147 bp
  20% of sequence in fragments shorter than    1475 bp
  25% of sequence in fragments shorter than    1820 bp
  30% of sequence in fragments shorter than    2175 bp
  35% of sequence in fragments shorter than    2562 bp
  40% of sequence in fragments shorter than    2970 bp
  45% of sequence in fragments shorter than    3412 bp
  50% of sequence in fragments shorter than    3886 bp
  55% of sequence in fragments shorter than    4400 bp
  60% of sequence in fragments shorter than    4996 bp
  65% of sequence in fragments shorter than    5668 bp
  70% of sequence in fragments shorter than    6438 bp
  75% of sequence in fragments shorter than    7359 bp
  80% of sequence in fragments shorter than    8484 bp
  85% of sequence in fragments shorter than    9974 bp
  90% of sequence in fragments shorter than   12094 bp
  95% of sequence in fragments shorter than   15946 bp

# AvrII.BsmI (CCTAGG+GAATGC) double digest, 2143 thousand fragments (594956+1549350 sites), 1390 bp average fragment length
   5% of sequence in fragments shorter than     464 bp
  10% of sequence in fragments shorter than     777 bp
  15% of sequence in fragments shorter than    1047 bp
  20% of sequence in fragments shorter than    1295 bp
  25% of sequence in fragments shorter than    1537 bp
  30% of sequence in fragments shorter than    1770 bp
  35% of sequence in fragments shorter than    2011 bp
  40% of sequence in fragments shorter than    2255 bp
  45% of sequence in fragments shorter than    2507 bp
  50% of sequence in fragments shorter than    2768 bp
  55% of sequence in fragments shorter than    3051 bp
  60% of sequence in fragments shorter than    3358 bp
  65% of sequence in fragments shorter than    3698 bp
  70% of sequence in fragments shorter than    4078 bp
  75% of sequence in fragments shorter than    4519 bp
  80% of sequence in fragments shorter than    5046 bp
  85% of sequence in fragments shorter than    5710 bp
  90% of sequence in fragments shorter than    6628 bp
  95% of sequence in fragments shorter than    8173 bp

# ApaLI.PsiI (GTGCAC+TTATAA) double digest, 2143 thousand fragments (496255+1647912 sites), 1390 bp average fragment length
   5% of sequence in fragments shorter than     468 bp
  10% of sequence in fragments shorter than     729 bp
  15% of sequence in fragments shorter than     954 bp
  20% of sequence in fragments shorter than    1169 bp
  25% of sequence in fragments shorter than    1379 bp
  30% of sequence in fragments shorter than    1594 bp
  35% of sequence in fragments shorter than    1814 bp
  40% of sequence in fragments shorter than    2044 bp
  45% of sequence in fragments shorter than    2288 bp
  50% of sequence in fragments shorter than    2544 bp
  55% of sequence in fragments shorter than    2820 bp
  60% of sequence in fragments shorter than    3109 bp
  65% of sequence in fragments shorter than    3430 bp
  70% of sequence in fragments shorter than    3819 bp
  75% of sequence in fragments shorter than    4287 bp
  80% of sequence in fragments shorter than    4876 bp
  85% of sequence in fragments shorter than    5648 bp
  90% of sequence in fragments shorter than    6780 bp
  95% of sequence in fragments shorter than    9037 bp

# BceAI.BspMI (ACGGC+ACCTGC) double digest, 2142 thousand fragments (705178+1438129 sites), 1390 bp average fragment length
   5% of sequence in fragments shorter than     454 bp
  10% of sequence in fragments shorter than     721 bp
  15% of sequence in fragments shorter than     961 bp
  20% of sequence in fragments shorter than    1202 bp
  25% of sequence in fragments shorter than    1431 bp
  30% of sequence in fragments shorter than    1676 bp
  35% of sequence in fragments shorter than    1928 bp
  40% of sequence in fragments shorter than    2167 bp
  45% of sequence in fragments shorter than    2444 bp
  50% of sequence in fragments shorter than    2740 bp
  55% of sequence in fragments shorter than    3056 bp
  60% of sequence in fragments shorter than    3367 bp
  65% of sequence in fragments shorter than    3756 bp
  70% of sequence in fragments shorter than    4200 bp
  75% of sequence in fragments shorter than    4725 bp
  80% of sequence in fragments shorter than    5348 bp
  85% of sequence in fragments shorter than    6149 bp
  90% of sequence in fragments shorter than    7290 bp
  95% of sequence in fragments shorter than    9240 bp

# BciVI.FauI (GTATCC+CCCGC) double digest, 2135 thousand fragments (696093+1439772 sites), 1395 bp average fragment length
   5% of sequence in fragments shorter than     486 bp
  10% of sequence in fragments shorter than     809 bp
  15% of sequence in fragments shorter than    1093 bp
  20% of sequence in fragments shorter than    1365 bp
  25% of sequence in fragments shorter than    1626 bp
  30% of sequence in fragments shorter than    1887 bp
  35% of sequence in fragments shorter than    2158 bp
  40% of sequence in fragments shorter than    2449 bp
  45% of sequence in fragments shorter than    2760 bp
  50% of sequence in fragments shorter than    3094 bp
  55% of sequence in fragments shorter than    3451 bp
  60% of sequence in fragments shorter than    3841 bp
  65% of sequence in fragments shorter than    4270 bp
  70% of sequence in fragments shorter than    4770 bp
  75% of sequence in fragments shorter than    5353 bp
  80% of sequence in fragments shorter than    6067 bp
  85% of sequence in fragments shorter than    6998 bp
  90% of sequence in fragments shorter than    8308 bp
  95% of sequence in fragments shorter than   10690 bp

# BscGI.NdeI (CCCGT+CATATG) double digest, 2133 thousand fragments (1231070+903360 sites), 1396 bp average fragment length
   5% of sequence in fragments shorter than     431 bp
  10% of sequence in fragments shorter than     703 bp
  15% of sequence in fragments shorter than     942 bp
  20% of sequence in fragments shorter than    1166 bp
  25% of sequence in fragments shorter than    1384 bp
  30% of sequence in fragments shorter than    1608 bp
  35% of sequence in fragments shorter than    1838 bp
  40% of sequence in fragments shorter than    2075 bp
  45% of sequence in fragments shorter than    2322 bp
  50% of sequence in fragments shorter than    2583 bp
  55% of sequence in fragments shorter than    2862 bp
  60% of sequence in fragments shorter than    3163 bp
  65% of sequence in fragments shorter than    3491 bp
  70% of sequence in fragments shorter than    3856 bp
  75% of sequence in fragments shorter than    4286 bp
  80% of sequence in fragments shorter than    4794 bp
  85% of sequence in fragments shorter than    5446 bp
  90% of sequence in fragments shorter than    6346 bp
  95% of sequence in fragments shorter than    7868 bp

# BciVI.BspMI (GTATCC+ACCTGC) double digest, 2133 thousand fragments (696093+1438129 sites), 1396 bp average fragment length
   5% of sequence in fragments shorter than     480 bp
  10% of sequence in fragments shorter than     742 bp
  15% of sequence in fragments shorter than     964 bp
  20% of sequence in fragments shorter than    1179 bp
  25% of sequence in fragments shorter than    1384 bp
  30% of sequence in fragments shorter than    1581 bp
  35% of sequence in fragments shorter than    1771 bp
  40% of sequence in fragments shorter than    1980 bp
  45% of sequence in fragments shorter than    2205 bp
  50% of sequence in fragments shorter than    2447 bp
  55% of sequence in fragments shorter than    2710 bp
  60% of sequence in fragments shorter than    2991 bp
  65% of sequence in fragments shorter than    3303 bp
  70% of sequence in fragments shorter than    3655 bp
  75% of sequence in fragments shorter than    4069 bp
  80% of sequence in fragments shorter than    4571 bp
  85% of sequence in fragments shorter than    5208 bp
  90% of sequence in fragments shorter than    6100 bp
  95% of sequence in fragments shorter than    7636 bp

# HhaI.SapI (GCGC+GCTCTTC) double digest, 2133 thousand fragments (1756502+377161 sites), 1396 bp average fragment length
   5% of sequence in fragments shorter than     469 bp
  10% of sequence in fragments shorter than     790 bp
  15% of sequence in fragments shorter than    1108 bp
  20% of sequence in fragments shorter than    1421 bp
  25% of sequence in fragments shorter than    1746 bp
  30% of sequence in fragments shorter than    2073 bp
  35% of sequence in fragments shorter than    2426 bp
  40% of sequence in fragments shorter than    2796 bp
  45% of sequence in fragments shorter than    3198 bp
  50% of sequence in fragments shorter than    3628 bp
  55% of sequence in fragments shorter than    4091 bp
  60% of sequence in fragments shorter than    4608 bp
  65% of sequence in fragments shorter than    5200 bp
  70% of sequence in fragments shorter than    5877 bp
  75% of sequence in fragments shorter than    6678 bp
  80% of sequence in fragments shorter than    7651 bp
  85% of sequence in fragments shorter than    8914 bp
  90% of sequence in fragments shorter than   10694 bp
  95% of sequence in fragments shorter than   13791 bp

# HhaI.SmaI (GCGC+CCCGGG) double digest, 2132 thousand fragments (1756502+376940 sites), 1397 bp average fragment length
   5% of sequence in fragments shorter than     462 bp
  10% of sequence in fragments shorter than     832 bp
  15% of sequence in fragments shorter than    1195 bp
  20% of sequence in fragments shorter than    1574 bp
  25% of sequence in fragments shorter than    1965 bp
  30% of sequence in fragments shorter than    2392 bp
  35% of sequence in fragments shorter than    2849 bp
  40% of sequence in fragments shorter than    3349 bp
  45% of sequence in fragments shorter than    3891 bp
  50% of sequence in fragments shorter than    4483 bp
  55% of sequence in fragments shorter than    5151 bp
  60% of sequence in fragments shorter than    5888 bp
  65% of sequence in fragments shorter than    6730 bp
  70% of sequence in fragments shorter than    7713 bp
  75% of sequence in fragments shorter than    8873 bp
  80% of sequence in fragments shorter than   10321 bp
  85% of sequence in fragments shorter than   12180 bp
  90% of sequence in fragments shorter than   14840 bp
  95% of sequence in fragments shorter than   19489 bp

# AflII.AflIII (CTTAAG+ACRYGT) double digest, 2128 thousand fragments (643284+1485394 sites), 1400 bp average fragment length
   5% of sequence in fragments shorter than     493 bp
  10% of sequence in fragments shorter than     762 bp
  15% of sequence in fragments shorter than     995 bp
  20% of sequence in fragments shorter than    1211 bp
  25% of sequence in fragments shorter than    1424 bp
  30% of sequence in fragments shorter than    1623 bp
  35% of sequence in fragments shorter than    1834 bp
  40% of sequence in fragments shorter than    2049 bp
  45% of sequence in fragments shorter than    2274 bp
  50% of sequence in fragments shorter than    2523 bp
  55% of sequence in fragments shorter than    2781 bp
  60% of sequence in fragments shorter than    3067 bp
  65% of sequence in fragments shorter than    3383 bp
  70% of sequence in fragments shorter than    3744 bp
  75% of sequence in fragments shorter than    4172 bp
  80% of sequence in fragments shorter than    4682 bp
  85% of sequence in fragments shorter than    5343 bp
  90% of sequence in fragments shorter than    6290 bp
  95% of sequence in fragments shorter than    8083 bp

# HgaI.HgiCI (GACGC+GGYRCC) double digest, 2122 thousand fragments (571889+1550877 sites), 1404 bp average fragment length
   5% of sequence in fragments shorter than     451 bp
  10% of sequence in fragments shorter than     729 bp
  15% of sequence in fragments shorter than     987 bp
  20% of sequence in fragments shorter than    1222 bp
  25% of sequence in fragments shorter than    1465 bp
  30% of sequence in fragments shorter than    1716 bp
  35% of sequence in fragments shorter than    1982 bp
  40% of sequence in fragments shorter than    2265 bp
  45% of sequence in fragments shorter than    2567 bp
  50% of sequence in fragments shorter than    2880 bp
  55% of sequence in fragments shorter than    3227 bp
  60% of sequence in fragments shorter than    3620 bp
  65% of sequence in fragments shorter than    4066 bp
  70% of sequence in fragments shorter than    4572 bp
  75% of sequence in fragments shorter than    5179 bp
  80% of sequence in fragments shorter than    5926 bp
  85% of sequence in fragments shorter than    6915 bp
  90% of sequence in fragments shorter than    8355 bp
  95% of sequence in fragments shorter than   11169 bp

# BamHI.HhaI (GGATCC+GCGC) double digest, 2122 thousand fragments (365999+1756502 sites), 1404 bp average fragment length
   5% of sequence in fragments shorter than     468 bp
  10% of sequence in fragments shorter than     803 bp
  15% of sequence in fragments shorter than    1122 bp
  20% of sequence in fragments shorter than    1442 bp
  25% of sequence in fragments shorter than    1770 bp
  30% of sequence in fragments shorter than    2106 bp
  35% of sequence in fragments shorter than    2470 bp
  40% of sequence in fragments shorter than    2853 bp
  45% of sequence in fragments shorter than    3260 bp
  50% of sequence in fragments shorter than    3706 bp
  55% of sequence in fragments shorter than    4183 bp
  60% of sequence in fragments shorter than    4712 bp
  65% of sequence in fragments shorter than    5317 bp
  70% of sequence in fragments shorter than    6033 bp
  75% of sequence in fragments shorter than    6869 bp
  80% of sequence in fragments shorter than    7895 bp
  85% of sequence in fragments shorter than    9202 bp
  90% of sequence in fragments shorter than   11082 bp
  95% of sequence in fragments shorter than   14258 bp

# EciI.HgiCI (GGCGGA+GGYRCC) double digest, 2121 thousand fragments (571273+1550877 sites), 1404 bp average fragment length
   5% of sequence in fragments shorter than     460 bp
  10% of sequence in fragments shorter than     750 bp
  15% of sequence in fragments shorter than    1018 bp
  20% of sequence in fragments shorter than    1253 bp
  25% of sequence in fragments shorter than    1509 bp
  30% of sequence in fragments shorter than    1764 bp
  35% of sequence in fragments shorter than    2045 bp
  40% of sequence in fragments shorter than    2339 bp
  45% of sequence in fragments shorter than    2657 bp
  50% of sequence in fragments shorter than    2978 bp
  55% of sequence in fragments shorter than    3349 bp
  60% of sequence in fragments shorter than    3771 bp
  65% of sequence in fragments shorter than    4241 bp
  70% of sequence in fragments shorter than    4789 bp
  75% of sequence in fragments shorter than    5446 bp
  80% of sequence in fragments shorter than    6251 bp
  85% of sequence in fragments shorter than    7347 bp
  90% of sequence in fragments shorter than    8968 bp
  95% of sequence in fragments shorter than   12332 bp

# BsmI.HgaI (GAATGC+GACGC) double digest, 2120 thousand fragments (1549350+571889 sites), 1405 bp average fragment length
   5% of sequence in fragments shorter than     453 bp
  10% of sequence in fragments shorter than     795 bp
  15% of sequence in fragments shorter than    1077 bp
  20% of sequence in fragments shorter than    1338 bp
  25% of sequence in fragments shorter than    1598 bp
  30% of sequence in fragments shorter than    1851 bp
  35% of sequence in fragments shorter than    2105 bp
  40% of sequence in fragments shorter than    2372 bp
  45% of sequence in fragments shorter than    2642 bp
  50% of sequence in fragments shorter than    2929 bp
  55% of sequence in fragments shorter than    3241 bp
  60% of sequence in fragments shorter than    3579 bp
  65% of sequence in fragments shorter than    3948 bp
  70% of sequence in fragments shorter than    4366 bp
  75% of sequence in fragments shorter than    4849 bp
  80% of sequence in fragments shorter than    5435 bp
  85% of sequence in fragments shorter than    6171 bp
  90% of sequence in fragments shorter than    7176 bp
  95% of sequence in fragments shorter than    8870 bp

# BsmI.EciI (GAATGC+GGCGGA) double digest, 2120 thousand fragments (1549350+571273 sites), 1405 bp average fragment length
   5% of sequence in fragments shorter than     455 bp
  10% of sequence in fragments shorter than     790 bp
  15% of sequence in fragments shorter than    1059 bp
  20% of sequence in fragments shorter than    1312 bp
  25% of sequence in fragments shorter than    1559 bp
  30% of sequence in fragments shorter than    1804 bp
  35% of sequence in fragments shorter than    2052 bp
  40% of sequence in fragments shorter than    2304 bp
  45% of sequence in fragments shorter than    2562 bp
  50% of sequence in fragments shorter than    2835 bp
  55% of sequence in fragments shorter than    3131 bp
  60% of sequence in fragments shorter than    3454 bp
  65% of sequence in fragments shorter than    3811 bp
  70% of sequence in fragments shorter than    4210 bp
  75% of sequence in fragments shorter than    4675 bp
  80% of sequence in fragments shorter than    5236 bp
  85% of sequence in fragments shorter than    5941 bp
  90% of sequence in fragments shorter than    6925 bp
  95% of sequence in fragments shorter than    8572 bp

# AflIII.HaeII (ACRYGT+RGCGCY) double digest, 2115 thousand fragments (1485394+630101 sites), 1408 bp average fragment length
   5% of sequence in fragments shorter than     473 bp
  10% of sequence in fragments shorter than     747 bp
  15% of sequence in fragments shorter than    1002 bp
  20% of sequence in fragments shorter than    1234 bp
  25% of sequence in fragments shorter than    1463 bp
  30% of sequence in fragments shorter than    1698 bp
  35% of sequence in fragments shorter than    1929 bp
  40% of sequence in fragments shorter than    2170 bp
  45% of sequence in fragments shorter than    2423 bp
  50% of sequence in fragments shorter than    2691 bp
  55% of sequence in fragments shorter than    2975 bp
  60% of sequence in fragments shorter than    3284 bp
  65% of sequence in fragments shorter than    3625 bp
  70% of sequence in fragments shorter than    3999 bp
  75% of sequence in fragments shorter than    4446 bp
  80% of sequence in fragments shorter than    4962 bp
  85% of sequence in fragments shorter than    5620 bp
  90% of sequence in fragments shorter than    6536 bp
  95% of sequence in fragments shorter than    8094 bp

# HgiCI.MfeI (GGYRCC+CAATTG) double digest, 2114 thousand fragments (1550877+564303 sites), 1409 bp average fragment length
   5% of sequence in fragments shorter than     475 bp
  10% of sequence in fragments shorter than     730 bp
  15% of sequence in fragments shorter than     947 bp
  20% of sequence in fragments shorter than    1162 bp
  25% of sequence in fragments shorter than    1367 bp
  30% of sequence in fragments shorter than    1578 bp
  35% of sequence in fragments shorter than    1787 bp
  40% of sequence in fragments shorter than    2009 bp
  45% of sequence in fragments shorter than    2239 bp
  50% of sequence in fragments shorter than    2488 bp
  55% of sequence in fragments shorter than    2758 bp
  60% of sequence in fragments shorter than    3042 bp
  65% of sequence in fragments shorter than    3367 bp
  70% of sequence in fragments shorter than    3737 bp
  75% of sequence in fragments shorter than    4172 bp
  80% of sequence in fragments shorter than    4701 bp
  85% of sequence in fragments shorter than    5392 bp
  90% of sequence in fragments shorter than    6388 bp
  95% of sequence in fragments shorter than    8273 bp

# BsmI.MfeI (GAATGC+CAATTG) double digest, 2113 thousand fragments (1549350+564303 sites), 1410 bp average fragment length
   5% of sequence in fragments shorter than     471 bp
  10% of sequence in fragments shorter than     801 bp
  15% of sequence in fragments shorter than    1074 bp
  20% of sequence in fragments shorter than    1325 bp
  25% of sequence in fragments shorter than    1572 bp
  30% of sequence in fragments shorter than    1812 bp
  35% of sequence in fragments shorter than    2055 bp
  40% of sequence in fragments shorter than    2304 bp
  45% of sequence in fragments shorter than    2558 bp
  50% of sequence in fragments shorter than    2826 bp
  55% of sequence in fragments shorter than    3117 bp
  60% of sequence in fragments shorter than    3436 bp
  65% of sequence in fragments shorter than    3790 bp
  70% of sequence in fragments shorter than    4190 bp
  75% of sequence in fragments shorter than    4654 bp
  80% of sequence in fragments shorter than    5204 bp
  85% of sequence in fragments shorter than    5910 bp
  90% of sequence in fragments shorter than    6897 bp
  95% of sequence in fragments shorter than    8603 bp

# ApaI.PsiI (GGGCCC+TTATAA) double digest, 2107 thousand fragments (460339+1647912 sites), 1413 bp average fragment length
   5% of sequence in fragments shorter than     487 bp
  10% of sequence in fragments shorter than     750 bp
  15% of sequence in fragments shorter than     980 bp
  20% of sequence in fragments shorter than    1197 bp
  25% of sequence in fragments shorter than    1410 bp
  30% of sequence in fragments shorter than    1627 bp
  35% of sequence in fragments shorter than    1849 bp
  40% of sequence in fragments shorter than    2078 bp
  45% of sequence in fragments shorter than    2321 bp
  50% of sequence in fragments shorter than    2578 bp
  55% of sequence in fragments shorter than    2858 bp
  60% of sequence in fragments shorter than    3145 bp
  65% of sequence in fragments shorter than    3486 bp
  70% of sequence in fragments shorter than    3871 bp
  75% of sequence in fragments shorter than    4324 bp
  80% of sequence in fragments shorter than    4884 bp
  85% of sequence in fragments shorter than    5622 bp
  90% of sequence in fragments shorter than    6697 bp
  95% of sequence in fragments shorter than    8875 bp

# BfiI.SpeI (ACTGGG+ACTAGT) double digest, 2106 thousand fragments (1706594+400286 sites), 1414 bp average fragment length
   5% of sequence in fragments shorter than     485 bp
  10% of sequence in fragments shorter than     749 bp
  15% of sequence in fragments shorter than     976 bp
  20% of sequence in fragments shorter than    1188 bp
  25% of sequence in fragments shorter than    1396 bp
  30% of sequence in fragments shorter than    1607 bp
  35% of sequence in fragments shorter than    1818 bp
  40% of sequence in fragments shorter than    2038 bp
  45% of sequence in fragments shorter than    2248 bp
  50% of sequence in fragments shorter than    2491 bp
  55% of sequence in fragments shorter than    2730 bp
  60% of sequence in fragments shorter than    3009 bp
  65% of sequence in fragments shorter than    3324 bp
  70% of sequence in fragments shorter than    3686 bp
  75% of sequence in fragments shorter than    4105 bp
  80% of sequence in fragments shorter than    4612 bp
  85% of sequence in fragments shorter than    5235 bp
  90% of sequence in fragments shorter than    6154 bp
  95% of sequence in fragments shorter than    7898 bp

# AccI.AvaI (GTMKAC+CYCGRG) double digest, 2106 thousand fragments (1101063+1005714 sites), 1414 bp average fragment length
   5% of sequence in fragments shorter than     490 bp
  10% of sequence in fragments shorter than     752 bp
  15% of sequence in fragments shorter than     986 bp
  20% of sequence in fragments shorter than    1207 bp
  25% of sequence in fragments shorter than    1417 bp
  30% of sequence in fragments shorter than    1637 bp
  35% of sequence in fragments shorter than    1860 bp
  40% of sequence in fragments shorter than    2089 bp
  45% of sequence in fragments shorter than    2333 bp
  50% of sequence in fragments shorter than    2590 bp
  55% of sequence in fragments shorter than    2864 bp
  60% of sequence in fragments shorter than    3173 bp
  65% of sequence in fragments shorter than    3513 bp
  70% of sequence in fragments shorter than    3900 bp
  75% of sequence in fragments shorter than    4350 bp
  80% of sequence in fragments shorter than    4838 bp
  85% of sequence in fragments shorter than    5517 bp
  90% of sequence in fragments shorter than    6490 bp
  95% of sequence in fragments shorter than    8288 bp

# HgiCI.SphI (GGYRCC+GCATGC) double digest, 2101 thousand fragments (1550877+550952 sites), 1418 bp average fragment length
   5% of sequence in fragments shorter than     469 bp
  10% of sequence in fragments shorter than     724 bp
  15% of sequence in fragments shorter than     957 bp
  20% of sequence in fragments shorter than    1177 bp
  25% of sequence in fragments shorter than    1381 bp
  30% of sequence in fragments shorter than    1595 bp
  35% of sequence in fragments shorter than    1828 bp
  40% of sequence in fragments shorter than    2072 bp
  45% of sequence in fragments shorter than    2329 bp
  50% of sequence in fragments shorter than    2611 bp
  55% of sequence in fragments shorter than    2901 bp
  60% of sequence in fragments shorter than    3233 bp
  65% of sequence in fragments shorter than    3611 bp
  70% of sequence in fragments shorter than    4050 bp
  75% of sequence in fragments shorter than    4564 bp
  80% of sequence in fragments shorter than    5207 bp
  85% of sequence in fragments shorter than    6049 bp
  90% of sequence in fragments shorter than    7314 bp
  95% of sequence in fragments shorter than    9867 bp

# BsmI.SphI (GAATGC+GCATGC) double digest, 2099 thousand fragments (1549350+550952 sites), 1419 bp average fragment length
   5% of sequence in fragments shorter than     476 bp
  10% of sequence in fragments shorter than     810 bp
  15% of sequence in fragments shorter than    1079 bp
  20% of sequence in fragments shorter than    1329 bp
  25% of sequence in fragments shorter than    1572 bp
  30% of sequence in fragments shorter than    1809 bp
  35% of sequence in fragments shorter than    2049 bp
  40% of sequence in fragments shorter than    2294 bp
  45% of sequence in fragments shorter than    2546 bp
  50% of sequence in fragments shorter than    2806 bp
  55% of sequence in fragments shorter than    3088 bp
  60% of sequence in fragments shorter than    3398 bp
  65% of sequence in fragments shorter than    3741 bp
  70% of sequence in fragments shorter than    4125 bp
  75% of sequence in fragments shorter than    4569 bp
  80% of sequence in fragments shorter than    5102 bp
  85% of sequence in fragments shorter than    5770 bp
  90% of sequence in fragments shorter than    6691 bp
  95% of sequence in fragments shorter than    8214 bp

# ApaLI.EsaBC3I (GTGCAC+TCGA) double digest, 2099 thousand fragments (496255+1603346 sites), 1419 bp average fragment length
   5% of sequence in fragments shorter than     488 bp
  10% of sequence in fragments shorter than     748 bp
  15% of sequence in fragments shorter than     973 bp
  20% of sequence in fragments shorter than    1188 bp
  25% of sequence in fragments shorter than    1390 bp
  30% of sequence in fragments shorter than    1606 bp
  35% of sequence in fragments shorter than    1825 bp
  40% of sequence in fragments shorter than    2054 bp
  45% of sequence in fragments shorter than    2292 bp
  50% of sequence in fragments shorter than    2544 bp
  55% of sequence in fragments shorter than    2808 bp
  60% of sequence in fragments shorter than    3105 bp
  65% of sequence in fragments shorter than    3438 bp
  70% of sequence in fragments shorter than    3819 bp
  75% of sequence in fragments shorter than    4259 bp
  80% of sequence in fragments shorter than    4792 bp
  85% of sequence in fragments shorter than    5488 bp
  90% of sequence in fragments shorter than    6459 bp
  95% of sequence in fragments shorter than    8130 bp

# BglII.PstI (AGATCT+CTGCAG) double digest, 2095 thousand fragments (774732+1321469 sites), 1421 bp average fragment length
   5% of sequence in fragments shorter than     493 bp
  10% of sequence in fragments shorter than     741 bp
  15% of sequence in fragments shorter than     967 bp
  20% of sequence in fragments shorter than    1187 bp
  25% of sequence in fragments shorter than    1394 bp
  30% of sequence in fragments shorter than    1610 bp
  35% of sequence in fragments shorter than    1831 bp
  40% of sequence in fragments shorter than    2062 bp
  45% of sequence in fragments shorter than    2296 bp
  50% of sequence in fragments shorter than    2555 bp
  55% of sequence in fragments shorter than    2829 bp
  60% of sequence in fragments shorter than    3132 bp
  65% of sequence in fragments shorter than    3467 bp
  70% of sequence in fragments shorter than    3849 bp
  75% of sequence in fragments shorter than    4291 bp
  80% of sequence in fragments shorter than    4826 bp
  85% of sequence in fragments shorter than    5510 bp
  90% of sequence in fragments shorter than    6492 bp
  95% of sequence in fragments shorter than    8315 bp

# BfiI.HpaI (ACTGGG+GTTAAC) double digest, 2095 thousand fragments (1706594+389418 sites), 1422 bp average fragment length
   5% of sequence in fragments shorter than     484 bp
  10% of sequence in fragments shorter than     747 bp
  15% of sequence in fragments shorter than     973 bp
  20% of sequence in fragments shorter than    1184 bp
  25% of sequence in fragments shorter than    1394 bp
  30% of sequence in fragments shorter than    1604 bp
  35% of sequence in fragments shorter than    1814 bp
  40% of sequence in fragments shorter than    2027 bp
  45% of sequence in fragments shorter than    2248 bp
  50% of sequence in fragments shorter than    2494 bp
  55% of sequence in fragments shorter than    2758 bp
  60% of sequence in fragments shorter than    3018 bp
  65% of sequence in fragments shorter than    3330 bp
  70% of sequence in fragments shorter than    3691 bp
  75% of sequence in fragments shorter than    4119 bp
  80% of sequence in fragments shorter than    4635 bp
  85% of sequence in fragments shorter than    5292 bp
  90% of sequence in fragments shorter than    6246 bp
  95% of sequence in fragments shorter than    8080 bp

# HgiCI.ScaI (GGYRCC+AGTACT) double digest, 2094 thousand fragments (1550877+543793 sites), 1422 bp average fragment length
   5% of sequence in fragments shorter than     484 bp
  10% of sequence in fragments shorter than     739 bp
  15% of sequence in fragments shorter than     968 bp
  20% of sequence in fragments shorter than    1183 bp
  25% of sequence in fragments shorter than    1379 bp
  30% of sequence in fragments shorter than    1585 bp
  35% of sequence in fragments shorter than    1802 bp
  40% of sequence in fragments shorter than    2030 bp
  45% of sequence in fragments shorter than    2271 bp
  50% of sequence in fragments shorter than    2527 bp
  55% of sequence in fragments shorter than    2797 bp
  60% of sequence in fragments shorter than    3088 bp
  65% of sequence in fragments shorter than    3423 bp
  70% of sequence in fragments shorter than    3811 bp
  75% of sequence in fragments shorter than    4261 bp
  80% of sequence in fragments shorter than    4816 bp
  85% of sequence in fragments shorter than    5551 bp
  90% of sequence in fragments shorter than    6609 bp
  95% of sequence in fragments shorter than    8785 bp

# EcoRV.PsiI (GATATC+TTATAA) double digest, 2093 thousand fragments (446473+1647912 sites), 1423 bp average fragment length
   5% of sequence in fragments shorter than     478 bp
  10% of sequence in fragments shorter than     733 bp
  15% of sequence in fragments shorter than     968 bp
  20% of sequence in fragments shorter than    1191 bp
  25% of sequence in fragments shorter than    1412 bp
  30% of sequence in fragments shorter than    1642 bp
  35% of sequence in fragments shorter than    1877 bp
  40% of sequence in fragments shorter than    2125 bp
  45% of sequence in fragments shorter than    2385 bp
  50% of sequence in fragments shorter than    2671 bp
  55% of sequence in fragments shorter than    2979 bp
  60% of sequence in fragments shorter than    3328 bp
  65% of sequence in fragments shorter than    3736 bp
  70% of sequence in fragments shorter than    4220 bp
  75% of sequence in fragments shorter than    4825 bp
  80% of sequence in fragments shorter than    5613 bp
  85% of sequence in fragments shorter than    6740 bp
  90% of sequence in fragments shorter than    8609 bp
  95% of sequence in fragments shorter than   13009 bp

# BsmI.ScaI (GAATGC+AGTACT) double digest, 2092 thousand fragments (1549350+543793 sites), 1424 bp average fragment length
   5% of sequence in fragments shorter than     476 bp
  10% of sequence in fragments shorter than     810 bp
  15% of sequence in fragments shorter than    1079 bp
  20% of sequence in fragments shorter than    1330 bp
  25% of sequence in fragments shorter than    1572 bp
  30% of sequence in fragments shorter than    1809 bp
  35% of sequence in fragments shorter than    2050 bp
  40% of sequence in fragments shorter than    2297 bp
  45% of sequence in fragments shorter than    2549 bp
  50% of sequence in fragments shorter than    2815 bp
  55% of sequence in fragments shorter than    3101 bp
  60% of sequence in fragments shorter than    3413 bp
  65% of sequence in fragments shorter than    3761 bp
  70% of sequence in fragments shorter than    4152 bp
  75% of sequence in fragments shorter than    4598 bp
  80% of sequence in fragments shorter than    5141 bp
  85% of sequence in fragments shorter than    5831 bp
  90% of sequence in fragments shorter than    6784 bp
  95% of sequence in fragments shorter than    8410 bp

# BstEII.HhaI (GGTNACC+GCGC) double digest, 2091 thousand fragments (335865+1756502 sites), 1424 bp average fragment length
   5% of sequence in fragments shorter than     474 bp
  10% of sequence in fragments shorter than     808 bp
  15% of sequence in fragments shorter than    1136 bp
  20% of sequence in fragments shorter than    1463 bp
  25% of sequence in fragments shorter than    1795 bp
  30% of sequence in fragments shorter than    2151 bp
  35% of sequence in fragments shorter than    2528 bp
  40% of sequence in fragments shorter than    2926 bp
  45% of sequence in fragments shorter than    3353 bp
  50% of sequence in fragments shorter than    3816 bp
  55% of sequence in fragments shorter than    4317 bp
  60% of sequence in fragments shorter than    4869 bp
  65% of sequence in fragments shorter than    5505 bp
  70% of sequence in fragments shorter than    6225 bp
  75% of sequence in fragments shorter than    7075 bp
  80% of sequence in fragments shorter than    8129 bp
  85% of sequence in fragments shorter than    9475 bp
  90% of sequence in fragments shorter than   11391 bp
  95% of sequence in fragments shorter than   14668 bp

# BscGI.HindIII (CCCGT+AAGCTT) double digest, 2090 thousand fragments (1231070+860365 sites), 1425 bp average fragment length
   5% of sequence in fragments shorter than     439 bp
  10% of sequence in fragments shorter than     713 bp
  15% of sequence in fragments shorter than     969 bp
  20% of sequence in fragments shorter than    1205 bp
  25% of sequence in fragments shorter than    1435 bp
  30% of sequence in fragments shorter than    1671 bp
  35% of sequence in fragments shorter than    1905 bp
  40% of sequence in fragments shorter than    2135 bp
  45% of sequence in fragments shorter than    2391 bp
  50% of sequence in fragments shorter than    2662 bp
  55% of sequence in fragments shorter than    2954 bp
  60% of sequence in fragments shorter than    3270 bp
  65% of sequence in fragments shorter than    3621 bp
  70% of sequence in fragments shorter than    4015 bp
  75% of sequence in fragments shorter than    4474 bp
  80% of sequence in fragments shorter than    5026 bp
  85% of sequence in fragments shorter than    5720 bp
  90% of sequence in fragments shorter than    6689 bp
  95% of sequence in fragments shorter than    8328 bp

# AvaI.PpuMI (CYCGRG+RGGWCCY) double digest, 2090 thousand fragments (1005714+1085138 sites), 1425 bp average fragment length
   5% of sequence in fragments shorter than     456 bp
  10% of sequence in fragments shorter than     745 bp
  15% of sequence in fragments shorter than    1014 bp
  20% of sequence in fragments shorter than    1283 bp
  25% of sequence in fragments shorter than    1559 bp
  30% of sequence in fragments shorter than    1840 bp
  35% of sequence in fragments shorter than    2133 bp
  40% of sequence in fragments shorter than    2448 bp
  45% of sequence in fragments shorter than    2786 bp
  50% of sequence in fragments shorter than    3131 bp
  55% of sequence in fragments shorter than    3525 bp
  60% of sequence in fragments shorter than    3957 bp
  65% of sequence in fragments shorter than    4465 bp
  70% of sequence in fragments shorter than    5063 bp
  75% of sequence in fragments shorter than    5770 bp
  80% of sequence in fragments shorter than    6640 bp
  85% of sequence in fragments shorter than    7821 bp
  90% of sequence in fragments shorter than    9575 bp
  95% of sequence in fragments shorter than   13285 bp

# AvaI.PvuII (CYCGRG+CAGCTG) double digest, 2089 thousand fragments (1005714+1084260 sites), 1426 bp average fragment length
   5% of sequence in fragments shorter than     460 bp
  10% of sequence in fragments shorter than     739 bp
  15% of sequence in fragments shorter than     994 bp
  20% of sequence in fragments shorter than    1248 bp
  25% of sequence in fragments shorter than    1505 bp
  30% of sequence in fragments shorter than    1772 bp
  35% of sequence in fragments shorter than    2053 bp
  40% of sequence in fragments shorter than    2353 bp
  45% of sequence in fragments shorter than    2675 bp
  50% of sequence in fragments shorter than    3018 bp
  55% of sequence in fragments shorter than    3410 bp
  60% of sequence in fragments shorter than    3848 bp
  65% of sequence in fragments shorter than    4345 bp
  70% of sequence in fragments shorter than    4922 bp
  75% of sequence in fragments shorter than    5597 bp
  80% of sequence in fragments shorter than    6431 bp
  85% of sequence in fragments shorter than    7522 bp
  90% of sequence in fragments shorter than    9176 bp
  95% of sequence in fragments shorter than   12502 bp

# AflIII.SacI (ACRYGT+GAGCTC) double digest, 2088 thousand fragments (1485394+603323 sites), 1427 bp average fragment length
   5% of sequence in fragments shorter than     505 bp
  10% of sequence in fragments shorter than     781 bp
  15% of sequence in fragments shorter than    1016 bp
  20% of sequence in fragments shorter than    1233 bp
  25% of sequence in fragments shorter than    1447 bp
  30% of sequence in fragments shorter than    1664 bp
  35% of sequence in fragments shorter than    1882 bp
  40% of sequence in fragments shorter than    2100 bp
  45% of sequence in fragments shorter than    2332 bp
  50% of sequence in fragments shorter than    2577 bp
  55% of sequence in fragments shorter than    2833 bp
  60% of sequence in fragments shorter than    3107 bp
  65% of sequence in fragments shorter than    3419 bp
  70% of sequence in fragments shorter than    3761 bp
  75% of sequence in fragments shorter than    4140 bp
  80% of sequence in fragments shorter than    4620 bp
  85% of sequence in fragments shorter than    5251 bp
  90% of sequence in fragments shorter than    6149 bp
  95% of sequence in fragments shorter than    7777 bp

# BfiI.SapI (ACTGGG+GCTCTTC) double digest, 2083 thousand fragments (1706594+377161 sites), 1430 bp average fragment length
   5% of sequence in fragments shorter than     478 bp
  10% of sequence in fragments shorter than     746 bp
  15% of sequence in fragments shorter than     967 bp
  20% of sequence in fragments shorter than    1185 bp
  25% of sequence in fragments shorter than    1400 bp
  30% of sequence in fragments shorter than    1622 bp
  35% of sequence in fragments shorter than    1843 bp
  40% of sequence in fragments shorter than    2075 bp
  45% of sequence in fragments shorter than    2319 bp
  50% of sequence in fragments shorter than    2582 bp
  55% of sequence in fragments shorter than    2838 bp
  60% of sequence in fragments shorter than    3149 bp
  65% of sequence in fragments shorter than    3487 bp
  70% of sequence in fragments shorter than    3879 bp
  75% of sequence in fragments shorter than    4341 bp
  80% of sequence in fragments shorter than    4899 bp
  85% of sequence in fragments shorter than    5636 bp
  90% of sequence in fragments shorter than    6705 bp
  95% of sequence in fragments shorter than    8798 bp

# BfiI.SmaI (ACTGGG+CCCGGG) double digest, 2083 thousand fragments (1706594+376940 sites), 1430 bp average fragment length
   5% of sequence in fragments shorter than     471 bp
  10% of sequence in fragments shorter than     737 bp
  15% of sequence in fragments shorter than     972 bp
  20% of sequence in fragments shorter than    1195 bp
  25% of sequence in fragments shorter than    1419 bp
  30% of sequence in fragments shorter than    1647 bp
  35% of sequence in fragments shorter than    1876 bp
  40% of sequence in fragments shorter than    2111 bp
  45% of sequence in fragments shorter than    2357 bp
  50% of sequence in fragments shorter than    2631 bp
  55% of sequence in fragments shorter than    2900 bp
  60% of sequence in fragments shorter than    3227 bp
  65% of sequence in fragments shorter than    3595 bp
  70% of sequence in fragments shorter than    4021 bp
  75% of sequence in fragments shorter than    4517 bp
  80% of sequence in fragments shorter than    5110 bp
  85% of sequence in fragments shorter than    5899 bp
  90% of sequence in fragments shorter than    7063 bp
  95% of sequence in fragments shorter than    9400 bp

# AflII.FauI (CTTAAG+CCCGC) double digest, 2082 thousand fragments (643284+1439772 sites), 1430 bp average fragment length
   5% of sequence in fragments shorter than     497 bp
  10% of sequence in fragments shorter than     826 bp
  15% of sequence in fragments shorter than    1118 bp
  20% of sequence in fragments shorter than    1391 bp
  25% of sequence in fragments shorter than    1673 bp
  30% of sequence in fragments shorter than    1950 bp
  35% of sequence in fragments shorter than    2235 bp
  40% of sequence in fragments shorter than    2538 bp
  45% of sequence in fragments shorter than    2856 bp
  50% of sequence in fragments shorter than    3201 bp
  55% of sequence in fragments shorter than    3572 bp
  60% of sequence in fragments shorter than    3954 bp
  65% of sequence in fragments shorter than    4397 bp
  70% of sequence in fragments shorter than    4912 bp
  75% of sequence in fragments shorter than    5521 bp
  80% of sequence in fragments shorter than    6268 bp
  85% of sequence in fragments shorter than    7231 bp
  90% of sequence in fragments shorter than    8626 bp
  95% of sequence in fragments shorter than   11298 bp

# BscGI.XbaI (CCCGT+TCTAGA) double digest, 2081 thousand fragments (1231070+850998 sites), 1431 bp average fragment length
   5% of sequence in fragments shorter than     447 bp
  10% of sequence in fragments shorter than     739 bp
  15% of sequence in fragments shorter than     988 bp
  20% of sequence in fragments shorter than    1229 bp
  25% of sequence in fragments shorter than    1451 bp
  30% of sequence in fragments shorter than    1687 bp
  35% of sequence in fragments shorter than    1923 bp
  40% of sequence in fragments shorter than    2174 bp
  45% of sequence in fragments shorter than    2422 bp
  50% of sequence in fragments shorter than    2695 bp
  55% of sequence in fragments shorter than    2988 bp
  60% of sequence in fragments shorter than    3306 bp
  65% of sequence in fragments shorter than    3653 bp
  70% of sequence in fragments shorter than    4055 bp
  75% of sequence in fragments shorter than    4520 bp
  80% of sequence in fragments shorter than    5078 bp
  85% of sequence in fragments shorter than    5782 bp
  90% of sequence in fragments shorter than    6759 bp
  95% of sequence in fragments shorter than    8385 bp

# AflII.BspMI (CTTAAG+ACCTGC) double digest, 2080 thousand fragments (643284+1438129 sites), 1432 bp average fragment length
   5% of sequence in fragments shorter than     494 bp
  10% of sequence in fragments shorter than     758 bp
  15% of sequence in fragments shorter than     981 bp
  20% of sequence in fragments shorter than    1198 bp
  25% of sequence in fragments shorter than    1401 bp
  30% of sequence in fragments shorter than    1601 bp
  35% of sequence in fragments shorter than    1818 bp
  40% of sequence in fragments shorter than    2000 bp
  45% of sequence in fragments shorter than    2230 bp
  50% of sequence in fragments shorter than    2475 bp
  55% of sequence in fragments shorter than    2743 bp
  60% of sequence in fragments shorter than    3024 bp
  65% of sequence in fragments shorter than    3338 bp
  70% of sequence in fragments shorter than    3689 bp
  75% of sequence in fragments shorter than    4107 bp
  80% of sequence in fragments shorter than    4607 bp
  85% of sequence in fragments shorter than    5244 bp
  90% of sequence in fragments shorter than    6140 bp
  95% of sequence in fragments shorter than    7703 bp

# NcoI.PstI (CCATGG+CTGCAG) double digest, 2080 thousand fragments (759594+1321469 sites), 1432 bp average fragment length
   5% of sequence in fragments shorter than     477 bp
  10% of sequence in fragments shorter than     743 bp
  15% of sequence in fragments shorter than     988 bp
  20% of sequence in fragments shorter than    1219 bp
  25% of sequence in fragments shorter than    1441 bp
  30% of sequence in fragments shorter than    1680 bp
  35% of sequence in fragments shorter than    1922 bp
  40% of sequence in fragments shorter than    2171 bp
  45% of sequence in fragments shorter than    2440 bp
  50% of sequence in fragments shorter than    2729 bp
  55% of sequence in fragments shorter than    3036 bp
  60% of sequence in fragments shorter than    3376 bp
  65% of sequence in fragments shorter than    3759 bp
  70% of sequence in fragments shorter than    4188 bp
  75% of sequence in fragments shorter than    4641 bp
  80% of sequence in fragments shorter than    5232 bp
  85% of sequence in fragments shorter than    6001 bp
  90% of sequence in fragments shorter than    7133 bp
  95% of sequence in fragments shorter than    9197 bp

# AflIII.AvrII (ACRYGT+CCTAGG) double digest, 2079 thousand fragments (1485394+594956 sites), 1432 bp average fragment length
   5% of sequence in fragments shorter than     513 bp
  10% of sequence in fragments shorter than     769 bp
  15% of sequence in fragments shorter than    1011 bp
  20% of sequence in fragments shorter than    1231 bp
  25% of sequence in fragments shorter than    1449 bp
  30% of sequence in fragments shorter than    1670 bp
  35% of sequence in fragments shorter than    1890 bp
  40% of sequence in fragments shorter than    2113 bp
  45% of sequence in fragments shorter than    2350 bp
  50% of sequence in fragments shorter than    2601 bp
  55% of sequence in fragments shorter than    2866 bp
  60% of sequence in fragments shorter than    3156 bp
  65% of sequence in fragments shorter than    3480 bp
  70% of sequence in fragments shorter than    3850 bp
  75% of sequence in fragments shorter than    4277 bp
  80% of sequence in fragments shorter than    4785 bp
  85% of sequence in fragments shorter than    5431 bp
  90% of sequence in fragments shorter than    6345 bp
  95% of sequence in fragments shorter than    8015 bp

# AccI.BspHI (GTMKAC+TCATGA) double digest, 2078 thousand fragments (1101063+978290 sites), 1433 bp average fragment length
   5% of sequence in fragments shorter than     507 bp
  10% of sequence in fragments shorter than     759 bp
  15% of sequence in fragments shorter than     983 bp
  20% of sequence in fragments shorter than    1188 bp
  25% of sequence in fragments shorter than    1385 bp
  30% of sequence in fragments shorter than    1589 bp
  35% of sequence in fragments shorter than    1783 bp
  40% of sequence in fragments shorter than    1992 bp
  45% of sequence in fragments shorter than    2211 bp
  50% of sequence in fragments shorter than    2449 bp
  55% of sequence in fragments shorter than    2705 bp
  60% of sequence in fragments shorter than    2982 bp
  65% of sequence in fragments shorter than    3293 bp
  70% of sequence in fragments shorter than    3650 bp
  75% of sequence in fragments shorter than    4065 bp
  80% of sequence in fragments shorter than    4535 bp
  85% of sequence in fragments shorter than    5181 bp
  90% of sequence in fragments shorter than    6115 bp
  95% of sequence in fragments shorter than    7869 bp

# BscGI.EcoRI (CCCGT+GAATTC) double digest, 2077 thousand fragments (1231070+847401 sites), 1434 bp average fragment length
   5% of sequence in fragments shorter than     442 bp
  10% of sequence in fragments shorter than     732 bp
  15% of sequence in fragments shorter than     995 bp
  20% of sequence in fragments shorter than    1243 bp
  25% of sequence in fragments shorter than    1483 bp
  30% of sequence in fragments shorter than    1726 bp
  35% of sequence in fragments shorter than    1973 bp
  40% of sequence in fragments shorter than    2224 bp
  45% of sequence in fragments shorter than    2488 bp
  50% of sequence in fragments shorter than    2770 bp
  55% of sequence in fragments shorter than    3071 bp
  60% of sequence in fragments shorter than    3397 bp
  65% of sequence in fragments shorter than    3763 bp
  70% of sequence in fragments shorter than    4177 bp
  75% of sequence in fragments shorter than    4658 bp
  80% of sequence in fragments shorter than    5234 bp
  85% of sequence in fragments shorter than    5960 bp
  90% of sequence in fragments shorter than    6974 bp
  95% of sequence in fragments shorter than    8684 bp

# BamHI.BfiI (GGATCC+ACTGGG) double digest, 2072 thousand fragments (365999+1706594 sites), 1438 bp average fragment length
   5% of sequence in fragments shorter than     486 bp
  10% of sequence in fragments shorter than     741 bp
  15% of sequence in fragments shorter than     979 bp
  20% of sequence in fragments shorter than    1202 bp
  25% of sequence in fragments shorter than    1426 bp
  30% of sequence in fragments shorter than    1648 bp
  35% of sequence in fragments shorter than    1876 bp
  40% of sequence in fragments shorter than    2096 bp
  45% of sequence in fragments shorter than    2327 bp
  50% of sequence in fragments shorter than    2596 bp
  55% of sequence in fragments shorter than    2882 bp
  60% of sequence in fragments shorter than    3199 bp
  65% of sequence in fragments shorter than    3554 bp
  70% of sequence in fragments shorter than    3963 bp
  75% of sequence in fragments shorter than    4432 bp
  80% of sequence in fragments shorter than    5007 bp
  85% of sequence in fragments shorter than    5748 bp
  90% of sequence in fragments shorter than    6848 bp
  95% of sequence in fragments shorter than    8970 bp

# FauI.HaeII (CCCGC+RGCGCY) double digest, 2069 thousand fragments (1439772+630101 sites), 1440 bp average fragment length
   5% of sequence in fragments shorter than     484 bp
  10% of sequence in fragments shorter than     863 bp
  15% of sequence in fragments shorter than    1249 bp
  20% of sequence in fragments shorter than    1648 bp
  25% of sequence in fragments shorter than    2063 bp
  30% of sequence in fragments shorter than    2507 bp
  35% of sequence in fragments shorter than    2979 bp
  40% of sequence in fragments shorter than    3491 bp
  45% of sequence in fragments shorter than    4041 bp
  50% of sequence in fragments shorter than    4645 bp
  55% of sequence in fragments shorter than    5326 bp
  60% of sequence in fragments shorter than    6078 bp
  65% of sequence in fragments shorter than    6939 bp
  70% of sequence in fragments shorter than    7938 bp
  75% of sequence in fragments shorter than    9118 bp
  80% of sequence in fragments shorter than   10567 bp
  85% of sequence in fragments shorter than   12461 bp
  90% of sequence in fragments shorter than   15129 bp
  95% of sequence in fragments shorter than   19983 bp

# BspMI.HaeII (ACCTGC+RGCGCY) double digest, 2067 thousand fragments (1438129+630101 sites), 1441 bp average fragment length
   5% of sequence in fragments shorter than     460 bp
  10% of sequence in fragments shorter than     747 bp
  15% of sequence in fragments shorter than    1009 bp
  20% of sequence in fragments shorter than    1264 bp
  25% of sequence in fragments shorter than    1525 bp
  30% of sequence in fragments shorter than    1784 bp
  35% of sequence in fragments shorter than    2045 bp
  40% of sequence in fragments shorter than    2325 bp
  45% of sequence in fragments shorter than    2628 bp
  50% of sequence in fragments shorter than    2950 bp
  55% of sequence in fragments shorter than    3274 bp
  60% of sequence in fragments shorter than    3635 bp
  65% of sequence in fragments shorter than    4063 bp
  70% of sequence in fragments shorter than    4550 bp
  75% of sequence in fragments shorter than    5112 bp
  80% of sequence in fragments shorter than    5797 bp
  85% of sequence in fragments shorter than    6669 bp
  90% of sequence in fragments shorter than    7905 bp
  95% of sequence in fragments shorter than   10002 bp

# ApaI.EsaBC3I (GGGCCC+TCGA) double digest, 2063 thousand fragments (460339+1603346 sites), 1444 bp average fragment length
   5% of sequence in fragments shorter than     479 bp
  10% of sequence in fragments shorter than     745 bp
  15% of sequence in fragments shorter than     983 bp
  20% of sequence in fragments shorter than    1211 bp
  25% of sequence in fragments shorter than    1431 bp
  30% of sequence in fragments shorter than    1667 bp
  35% of sequence in fragments shorter than    1907 bp
  40% of sequence in fragments shorter than    2162 bp
  45% of sequence in fragments shorter than    2434 bp
  50% of sequence in fragments shorter than    2722 bp
  55% of sequence in fragments shorter than    3027 bp
  60% of sequence in fragments shorter than    3368 bp
  65% of sequence in fragments shorter than    3751 bp
  70% of sequence in fragments shorter than    4188 bp
  75% of sequence in fragments shorter than    4707 bp
  80% of sequence in fragments shorter than    5334 bp
  85% of sequence in fragments shorter than    6135 bp
  90% of sequence in fragments shorter than    7264 bp
  95% of sequence in fragments shorter than    9190 bp

# BspHI.PpuMI (TCATGA+RGGWCCY) double digest, 2063 thousand fragments (978290+1085138 sites), 1444 bp average fragment length
   5% of sequence in fragments shorter than     501 bp
  10% of sequence in fragments shorter than     770 bp
  15% of sequence in fragments shorter than    1008 bp
  20% of sequence in fragments shorter than    1233 bp
  25% of sequence in fragments shorter than    1456 bp
  30% of sequence in fragments shorter than    1682 bp
  35% of sequence in fragments shorter than    1906 bp
  40% of sequence in fragments shorter than    2132 bp
  45% of sequence in fragments shorter than    2375 bp
  50% of sequence in fragments shorter than    2636 bp
  55% of sequence in fragments shorter than    2919 bp
  60% of sequence in fragments shorter than    3215 bp
  65% of sequence in fragments shorter than    3531 bp
  70% of sequence in fragments shorter than    3903 bp
  75% of sequence in fragments shorter than    4352 bp
  80% of sequence in fragments shorter than    4903 bp
  85% of sequence in fragments shorter than    5623 bp
  90% of sequence in fragments shorter than    6677 bp
  95% of sequence in fragments shorter than    8745 bp

# BspHI.PvuII (TCATGA+CAGCTG) double digest, 2062 thousand fragments (978290+1084260 sites), 1445 bp average fragment length
   5% of sequence in fragments shorter than     506 bp
  10% of sequence in fragments shorter than     766 bp
  15% of sequence in fragments shorter than     991 bp
  20% of sequence in fragments shorter than    1205 bp
  25% of sequence in fragments shorter than    1414 bp
  30% of sequence in fragments shorter than    1627 bp
  35% of sequence in fragments shorter than    1843 bp
  40% of sequence in fragments shorter than    2063 bp
  45% of sequence in fragments shorter than    2287 bp
  50% of sequence in fragments shorter than    2530 bp
  55% of sequence in fragments shorter than    2784 bp
  60% of sequence in fragments shorter than    3064 bp
  65% of sequence in fragments shorter than    3381 bp
  70% of sequence in fragments shorter than    3715 bp
  75% of sequence in fragments shorter than    4131 bp
  80% of sequence in fragments shorter than    4634 bp
  85% of sequence in fragments shorter than    5294 bp
  90% of sequence in fragments shorter than    6239 bp
  95% of sequence in fragments shorter than    8041 bp

# BclI.PstI (TGATCA+CTGCAG) double digest, 2059 thousand fragments (738785+1321469 sites), 1446 bp average fragment length
   5% of sequence in fragments shorter than     504 bp
  10% of sequence in fragments shorter than     767 bp
  15% of sequence in fragments shorter than     999 bp
  20% of sequence in fragments shorter than    1218 bp
  25% of sequence in fragments shorter than    1432 bp
  30% of sequence in fragments shorter than    1650 bp
  35% of sequence in fragments shorter than    1872 bp
  40% of sequence in fragments shorter than    2095 bp
  45% of sequence in fragments shorter than    2324 bp
  50% of sequence in fragments shorter than    2580 bp
  55% of sequence in fragments shorter than    2851 bp
  60% of sequence in fragments shorter than    3144 bp
  65% of sequence in fragments shorter than    3473 bp
  70% of sequence in fragments shorter than    3854 bp
  75% of sequence in fragments shorter than    4290 bp
  80% of sequence in fragments shorter than    4825 bp
  85% of sequence in fragments shorter than    5509 bp
  90% of sequence in fragments shorter than    6491 bp
  95% of sequence in fragments shorter than    8279 bp

# AflIII.HgaI (ACRYGT+GACGC) double digest, 2056 thousand fragments (1485394+571889 sites), 1448 bp average fragment length
   5% of sequence in fragments shorter than     506 bp
  10% of sequence in fragments shorter than     799 bp
  15% of sequence in fragments shorter than    1054 bp
  20% of sequence in fragments shorter than    1291 bp
  25% of sequence in fragments shorter than    1524 bp
  30% of sequence in fragments shorter than    1757 bp
  35% of sequence in fragments shorter than    1989 bp
  40% of sequence in fragments shorter than    2228 bp
  45% of sequence in fragments shorter than    2476 bp
  50% of sequence in fragments shorter than    2743 bp
  55% of sequence in fragments shorter than    3018 bp
  60% of sequence in fragments shorter than    3325 bp
  65% of sequence in fragments shorter than    3672 bp
  70% of sequence in fragments shorter than    4064 bp
  75% of sequence in fragments shorter than    4518 bp
  80% of sequence in fragments shorter than    5065 bp
  85% of sequence in fragments shorter than    5751 bp
  90% of sequence in fragments shorter than    6734 bp
  95% of sequence in fragments shorter than    8502 bp

# AflIII.EciI (ACRYGT+GGCGGA) double digest, 2056 thousand fragments (1485394+571273 sites), 1449 bp average fragment length
   5% of sequence in fragments shorter than     497 bp
  10% of sequence in fragments shorter than     784 bp
  15% of sequence in fragments shorter than    1031 bp
  20% of sequence in fragments shorter than    1261 bp
  25% of sequence in fragments shorter than    1488 bp
  30% of sequence in fragments shorter than    1716 bp
  35% of sequence in fragments shorter than    1942 bp
  40% of sequence in fragments shorter than    2175 bp
  45% of sequence in fragments shorter than    2421 bp
  50% of sequence in fragments shorter than    2686 bp
  55% of sequence in fragments shorter than    2960 bp
  60% of sequence in fragments shorter than    3266 bp
  65% of sequence in fragments shorter than    3610 bp
  70% of sequence in fragments shorter than    4005 bp
  75% of sequence in fragments shorter than    4462 bp
  80% of sequence in fragments shorter than    5022 bp
  85% of sequence in fragments shorter than    5741 bp
  90% of sequence in fragments shorter than    6740 bp
  95% of sequence in fragments shorter than    8554 bp

# FnuDII.PstI (CGCG+CTGCAG) double digest, 2054 thousand fragments (733940+1321469 sites), 1450 bp average fragment length
   5% of sequence in fragments shorter than     498 bp
  10% of sequence in fragments shorter than     816 bp
  15% of sequence in fragments shorter than    1114 bp
  20% of sequence in fragments shorter than    1399 bp
  25% of sequence in fragments shorter than    1694 bp
  30% of sequence in fragments shorter than    1995 bp
  35% of sequence in fragments shorter than    2303 bp
  40% of sequence in fragments shorter than    2640 bp
  45% of sequence in fragments shorter than    2995 bp
  50% of sequence in fragments shorter than    3382 bp
  55% of sequence in fragments shorter than    3808 bp
  60% of sequence in fragments shorter than    4280 bp
  65% of sequence in fragments shorter than    4813 bp
  70% of sequence in fragments shorter than    5415 bp
  75% of sequence in fragments shorter than    6115 bp
  80% of sequence in fragments shorter than    6979 bp
  85% of sequence in fragments shorter than    8095 bp
  90% of sequence in fragments shorter than    9741 bp
  95% of sequence in fragments shorter than   12714 bp

# HhaI.SexAI (GCGC+ACCWGGT) double digest, 2053 thousand fragments (1756502+297900 sites), 1450 bp average fragment length
   5% of sequence in fragments shorter than     487 bp
  10% of sequence in fragments shorter than     834 bp
  15% of sequence in fragments shorter than    1167 bp
  20% of sequence in fragments shorter than    1502 bp
  25% of sequence in fragments shorter than    1841 bp
  30% of sequence in fragments shorter than    2202 bp
  35% of sequence in fragments shorter than    2586 bp
  40% of sequence in fragments shorter than    2989 bp
  45% of sequence in fragments shorter than    3426 bp
  50% of sequence in fragments shorter than    3897 bp
  55% of sequence in fragments shorter than    4417 bp
  60% of sequence in fragments shorter than    5007 bp
  65% of sequence in fragments shorter than    5661 bp
  70% of sequence in fragments shorter than    6407 bp
  75% of sequence in fragments shorter than    7296 bp
  80% of sequence in fragments shorter than    8372 bp
  85% of sequence in fragments shorter than    9762 bp
  90% of sequence in fragments shorter than   11731 bp
  95% of sequence in fragments shorter than   15152 bp

# EcoRV.EsaBC3I (GATATC+TCGA) double digest, 2049 thousand fragments (446473+1603346 sites), 1454 bp average fragment length
   5% of sequence in fragments shorter than     502 bp
  10% of sequence in fragments shorter than     763 bp
  15% of sequence in fragments shorter than     994 bp
  20% of sequence in fragments shorter than    1216 bp
  25% of sequence in fragments shorter than    1422 bp
  30% of sequence in fragments shorter than    1641 bp
  35% of sequence in fragments shorter than    1863 bp
  40% of sequence in fragments shorter than    2091 bp
  45% of sequence in fragments shorter than    2332 bp
  50% of sequence in fragments shorter than    2587 bp
  55% of sequence in fragments shorter than    2859 bp
  60% of sequence in fragments shorter than    3151 bp
  65% of sequence in fragments shorter than    3476 bp
  70% of sequence in fragments shorter than    3842 bp
  75% of sequence in fragments shorter than    4267 bp
  80% of sequence in fragments shorter than    4780 bp
  85% of sequence in fragments shorter than    5438 bp
  90% of sequence in fragments shorter than    6354 bp
  95% of sequence in fragments shorter than    7912 bp

# AflIII.MfeI (ACRYGT+CAATTG) double digest, 2049 thousand fragments (1485394+564303 sites), 1454 bp average fragment length
   5% of sequence in fragments shorter than     515 bp
  10% of sequence in fragments shorter than     792 bp
  15% of sequence in fragments shorter than    1034 bp
  20% of sequence in fragments shorter than    1262 bp
  25% of sequence in fragments shorter than    1471 bp
  30% of sequence in fragments shorter than    1693 bp
  35% of sequence in fragments shorter than    1914 bp
  40% of sequence in fragments shorter than    2142 bp
  45% of sequence in fragments shorter than    2388 bp
  50% of sequence in fragments shorter than    2648 bp
  55% of sequence in fragments shorter than    2924 bp
  60% of sequence in fragments shorter than    3225 bp
  65% of sequence in fragments shorter than    3569 bp
  70% of sequence in fragments shorter than    3958 bp
  75% of sequence in fragments shorter than    4411 bp
  80% of sequence in fragments shorter than    4942 bp
  85% of sequence in fragments shorter than    5634 bp
  90% of sequence in fragments shorter than    6610 bp
  95% of sequence in fragments shorter than    8408 bp

# PsiI.SpeI (TTATAA+ACTAGT) double digest, 2047 thousand fragments (1647912+400286 sites), 1455 bp average fragment length
   5% of sequence in fragments shorter than     487 bp
  10% of sequence in fragments shorter than     758 bp
  15% of sequence in fragments shorter than     997 bp
  20% of sequence in fragments shorter than    1226 bp
  25% of sequence in fragments shorter than    1453 bp
  30% of sequence in fragments shorter than    1690 bp
  35% of sequence in fragments shorter than    1932 bp
  40% of sequence in fragments shorter than    2187 bp
  45% of sequence in fragments shorter than    2465 bp
  50% of sequence in fragments shorter than    2766 bp
  55% of sequence in fragments shorter than    3063 bp
  60% of sequence in fragments shorter than    3428 bp
  65% of sequence in fragments shorter than    3857 bp
  70% of sequence in fragments shorter than    4375 bp
  75% of sequence in fragments shorter than    5019 bp
  80% of sequence in fragments shorter than    5862 bp
  85% of sequence in fragments shorter than    7044 bp
  90% of sequence in fragments shorter than    9044 bp
  95% of sequence in fragments shorter than   14155 bp

# ApaLI.HgiCI (GTGCAC+GGYRCC) double digest, 2046 thousand fragments (496255+1550877 sites), 1455 bp average fragment length
   5% of sequence in fragments shorter than     483 bp
  10% of sequence in fragments shorter than     750 bp
  15% of sequence in fragments shorter than     993 bp
  20% of sequence in fragments shorter than    1218 bp
  25% of sequence in fragments shorter than    1417 bp
  30% of sequence in fragments shorter than    1643 bp
  35% of sequence in fragments shorter than    1891 bp
  40% of sequence in fragments shorter than    2149 bp
  45% of sequence in fragments shorter than    2425 bp
  50% of sequence in fragments shorter than    2714 bp
  55% of sequence in fragments shorter than    3020 bp
  60% of sequence in fragments shorter than    3370 bp
  65% of sequence in fragments shorter than    3769 bp
  70% of sequence in fragments shorter than    4226 bp
  75% of sequence in fragments shorter than    4771 bp
  80% of sequence in fragments shorter than    5449 bp
  85% of sequence in fragments shorter than    6338 bp
  90% of sequence in fragments shorter than    7658 bp
  95% of sequence in fragments shorter than   10256 bp

# ApaLI.BsmI (GTGCAC+GAATGC) double digest, 2045 thousand fragments (496255+1549350 sites), 1457 bp average fragment length
   5% of sequence in fragments shorter than     488 bp
  10% of sequence in fragments shorter than     835 bp
  15% of sequence in fragments shorter than    1111 bp
  20% of sequence in fragments shorter than    1370 bp
  25% of sequence in fragments shorter than    1622 bp
  30% of sequence in fragments shorter than    1844 bp
  35% of sequence in fragments shorter than    2091 bp
  40% of sequence in fragments shorter than    2349 bp
  45% of sequence in fragments shorter than    2611 bp
  50% of sequence in fragments shorter than    2885 bp
  55% of sequence in fragments shorter than    3183 bp
  60% of sequence in fragments shorter than    3509 bp
  65% of sequence in fragments shorter than    3869 bp
  70% of sequence in fragments shorter than    4272 bp
  75% of sequence in fragments shorter than    4734 bp
  80% of sequence in fragments shorter than    5289 bp
  85% of sequence in fragments shorter than    5984 bp
  90% of sequence in fragments shorter than    6951 bp
  95% of sequence in fragments shorter than    8578 bp

# HhaI.KpnI (GCGC+GGTACC) double digest, 2044 thousand fragments (1756502+287974 sites), 1457 bp average fragment length
   5% of sequence in fragments shorter than     499 bp
  10% of sequence in fragments shorter than     854 bp
  15% of sequence in fragments shorter than    1191 bp
  20% of sequence in fragments shorter than    1501 bp
  25% of sequence in fragments shorter than    1819 bp
  30% of sequence in fragments shorter than    2176 bp
  35% of sequence in fragments shorter than    2550 bp
  40% of sequence in fragments shorter than    2938 bp
  45% of sequence in fragments shorter than    3356 bp
  50% of sequence in fragments shorter than    3812 bp
  55% of sequence in fragments shorter than    4312 bp
  60% of sequence in fragments shorter than    4873 bp
  65% of sequence in fragments shorter than    5504 bp
  70% of sequence in fragments shorter than    6224 bp
  75% of sequence in fragments shorter than    7076 bp
  80% of sequence in fragments shorter than    8115 bp
  85% of sequence in fragments shorter than    9453 bp
  90% of sequence in fragments shorter than   11351 bp
  95% of sequence in fragments shorter than   14646 bp

# HhaI.NheI (GCGC+GCTAGC) double digest, 2043 thousand fragments (1756502+287384 sites), 1458 bp average fragment length
   5% of sequence in fragments shorter than     504 bp
  10% of sequence in fragments shorter than     861 bp
  15% of sequence in fragments shorter than    1192 bp
  20% of sequence in fragments shorter than    1535 bp
  25% of sequence in fragments shorter than    1845 bp
  30% of sequence in fragments shorter than    2188 bp
  35% of sequence in fragments shorter than    2564 bp
  40% of sequence in fragments shorter than    2937 bp
  45% of sequence in fragments shorter than    3359 bp
  50% of sequence in fragments shorter than    3814 bp
  55% of sequence in fragments shorter than    4314 bp
  60% of sequence in fragments shorter than    4872 bp
  65% of sequence in fragments shorter than    5503 bp
  70% of sequence in fragments shorter than    6227 bp
  75% of sequence in fragments shorter than    7072 bp
  80% of sequence in fragments shorter than    8106 bp
  85% of sequence in fragments shorter than    9419 bp
  90% of sequence in fragments shorter than   11309 bp
  95% of sequence in fragments shorter than   14532 bp

# FauI.SacI (CCCGC+GAGCTC) double digest, 2042 thousand fragments (1439772+603323 sites), 1458 bp average fragment length
   5% of sequence in fragments shorter than     493 bp
  10% of sequence in fragments shorter than     838 bp
  15% of sequence in fragments shorter than    1156 bp
  20% of sequence in fragments shorter than    1452 bp
  25% of sequence in fragments shorter than    1767 bp
  30% of sequence in fragments shorter than    2096 bp
  35% of sequence in fragments shorter than    2448 bp
  40% of sequence in fragments shorter than    2801 bp
  45% of sequence in fragments shorter than    3206 bp
  50% of sequence in fragments shorter than    3644 bp
  55% of sequence in fragments shorter than    4119 bp
  60% of sequence in fragments shorter than    4649 bp
  65% of sequence in fragments shorter than    5244 bp
  70% of sequence in fragments shorter than    5939 bp
  75% of sequence in fragments shorter than    6755 bp
  80% of sequence in fragments shorter than    7761 bp
  85% of sequence in fragments shorter than    9095 bp
  90% of sequence in fragments shorter than   11026 bp
  95% of sequence in fragments shorter than   14627 bp

# BfiI.BstEII (ACTGGG+GGTNACC) double digest, 2042 thousand fragments (1706594+335865 sites), 1459 bp average fragment length
   5% of sequence in fragments shorter than     492 bp
  10% of sequence in fragments shorter than     765 bp
  15% of sequence in fragments shorter than     998 bp
  20% of sequence in fragments shorter than    1208 bp
  25% of sequence in fragments shorter than    1433 bp
  30% of sequence in fragments shorter than    1660 bp
  35% of sequence in fragments shorter than    1889 bp
  40% of sequence in fragments shorter than    2125 bp
  45% of sequence in fragments shorter than    2381 bp
  50% of sequence in fragments shorter than    2654 bp
  55% of sequence in fragments shorter than    2920 bp
  60% of sequence in fragments shorter than    3240 bp
  65% of sequence in fragments shorter than    3597 bp
  70% of sequence in fragments shorter than    3996 bp
  75% of sequence in fragments shorter than    4469 bp
  80% of sequence in fragments shorter than    5055 bp
  85% of sequence in fragments shorter than    5816 bp
  90% of sequence in fragments shorter than    6927 bp
  95% of sequence in fragments shorter than    9150 bp

# BspMI.SacI (ACCTGC+GAGCTC) double digest, 2040 thousand fragments (1438129+603323 sites), 1460 bp average fragment length
   5% of sequence in fragments shorter than     489 bp
  10% of sequence in fragments shorter than     762 bp
  15% of sequence in fragments shorter than     991 bp
  20% of sequence in fragments shorter than    1217 bp
  25% of sequence in fragments shorter than    1441 bp
  30% of sequence in fragments shorter than    1679 bp
  35% of sequence in fragments shorter than    1922 bp
  40% of sequence in fragments shorter than    2170 bp
  45% of sequence in fragments shorter than    2437 bp
  50% of sequence in fragments shorter than    2726 bp
  55% of sequence in fragments shorter than    3032 bp
  60% of sequence in fragments shorter than    3333 bp
  65% of sequence in fragments shorter than    3710 bp
  70% of sequence in fragments shorter than    4139 bp
  75% of sequence in fragments shorter than    4630 bp
  80% of sequence in fragments shorter than    5238 bp
  85% of sequence in fragments shorter than    6018 bp
  90% of sequence in fragments shorter than    7126 bp
  95% of sequence in fragments shorter than    9069 bp

# HpaI.PsiI (GTTAAC+TTATAA) double digest, 2036 thousand fragments (389418+1647912 sites), 1462 bp average fragment length
   5% of sequence in fragments shorter than     487 bp
  10% of sequence in fragments shorter than     758 bp
  15% of sequence in fragments shorter than    1000 bp
  20% of sequence in fragments shorter than    1233 bp
  25% of sequence in fragments shorter than    1462 bp
  30% of sequence in fragments shorter than    1700 bp
  35% of sequence in fragments shorter than    1946 bp
  40% of sequence in fragments shorter than    2208 bp
  45% of sequence in fragments shorter than    2491 bp
  50% of sequence in fragments shorter than    2793 bp
  55% of sequence in fragments shorter than    3102 bp
  60% of sequence in fragments shorter than    3470 bp
  65% of sequence in fragments shorter than    3893 bp
  70% of sequence in fragments shorter than    4399 bp
  75% of sequence in fragments shorter than    5034 bp
  80% of sequence in fragments shorter than    5856 bp
  85% of sequence in fragments shorter than    7016 bp
  90% of sequence in fragments shorter than    8989 bp
  95% of sequence in fragments shorter than   14117 bp

# AflIII.SphI (ACRYGT+GCATGC) double digest, 2035 thousand fragments (1485394+550952 sites), 1463 bp average fragment length
   5% of sequence in fragments shorter than     518 bp
  10% of sequence in fragments shorter than     803 bp
  15% of sequence in fragments shorter than    1049 bp
  20% of sequence in fragments shorter than    1278 bp
  25% of sequence in fragments shorter than    1502 bp
  30% of sequence in fragments shorter than    1723 bp
  35% of sequence in fragments shorter than    1945 bp
  40% of sequence in fragments shorter than    2172 bp
  45% of sequence in fragments shorter than    2412 bp
  50% of sequence in fragments shorter than    2668 bp
  55% of sequence in fragments shorter than    2936 bp
  60% of sequence in fragments shorter than    3233 bp
  65% of sequence in fragments shorter than    3563 bp
  70% of sequence in fragments shorter than    3938 bp
  75% of sequence in fragments shorter than    4379 bp
  80% of sequence in fragments shorter than    4898 bp
  85% of sequence in fragments shorter than    5568 bp
  90% of sequence in fragments shorter than    6514 bp
  95% of sequence in fragments shorter than    8207 bp

# BsaAI.PstI (YACGTR+CTGCAG) double digest, 2034 thousand fragments (713801+1321469 sites), 1464 bp average fragment length
   5% of sequence in fragments shorter than     496 bp
  10% of sequence in fragments shorter than     763 bp
  15% of sequence in fragments shorter than    1006 bp
  20% of sequence in fragments shorter than    1231 bp
  25% of sequence in fragments shorter than    1453 bp
  30% of sequence in fragments shorter than    1688 bp
  35% of sequence in fragments shorter than    1921 bp
  40% of sequence in fragments shorter than    2170 bp
  45% of sequence in fragments shorter than    2431 bp
  50% of sequence in fragments shorter than    2714 bp
  55% of sequence in fragments shorter than    3013 bp
  60% of sequence in fragments shorter than    3346 bp
  65% of sequence in fragments shorter than    3699 bp
  70% of sequence in fragments shorter than    4115 bp
  75% of sequence in fragments shorter than    4587 bp
  80% of sequence in fragments shorter than    5171 bp
  85% of sequence in fragments shorter than    5921 bp
  90% of sequence in fragments shorter than    6979 bp
  95% of sequence in fragments shorter than    8838 bp

# AvrII.FauI (CCTAGG+CCCGC) double digest, 2034 thousand fragments (594956+1439772 sites), 1464 bp average fragment length
   5% of sequence in fragments shorter than     493 bp
  10% of sequence in fragments shorter than     831 bp
  15% of sequence in fragments shorter than    1149 bp
  20% of sequence in fragments shorter than    1455 bp
  25% of sequence in fragments shorter than    1771 bp
  30% of sequence in fragments shorter than    2088 bp
  35% of sequence in fragments shorter than    2428 bp
  40% of sequence in fragments shorter than    2783 bp
  45% of sequence in fragments shorter than    3168 bp
  50% of sequence in fragments shorter than    3580 bp
  55% of sequence in fragments shorter than    4005 bp
  60% of sequence in fragments shorter than    4475 bp
  65% of sequence in fragments shorter than    5028 bp
  70% of sequence in fragments shorter than    5672 bp
  75% of sequence in fragments shorter than    6438 bp
  80% of sequence in fragments shorter than    7373 bp
  85% of sequence in fragments shorter than    8580 bp
  90% of sequence in fragments shorter than   10366 bp
  95% of sequence in fragments shorter than   13777 bp

# AccI.AvaIII (GTMKAC+ATGCAT) double digest, 2033 thousand fragments (1101063+933357 sites), 1465 bp average fragment length
   5% of sequence in fragments shorter than     513 bp
  10% of sequence in fragments shorter than     785 bp
  15% of sequence in fragments shorter than    1020 bp
  20% of sequence in fragments shorter than    1239 bp
  25% of sequence in fragments shorter than    1452 bp
  30% of sequence in fragments shorter than    1668 bp
  35% of sequence in fragments shorter than    1889 bp
  40% of sequence in fragments shorter than    2110 bp
  45% of sequence in fragments shorter than    2352 bp
  50% of sequence in fragments shorter than    2604 bp
  55% of sequence in fragments shorter than    2879 bp
  60% of sequence in fragments shorter than    3183 bp
  65% of sequence in fragments shorter than    3518 bp
  70% of sequence in fragments shorter than    3904 bp
  75% of sequence in fragments shorter than    4359 bp
  80% of sequence in fragments shorter than    4908 bp
  85% of sequence in fragments shorter than    5614 bp
  90% of sequence in fragments shorter than    6558 bp
  95% of sequence in fragments shorter than    8450 bp

# AvrII.BspMI (CCTAGG+ACCTGC) double digest, 2032 thousand fragments (594956+1438129 sites), 1466 bp average fragment length
   5% of sequence in fragments shorter than     502 bp
  10% of sequence in fragments shorter than     764 bp
  15% of sequence in fragments shorter than    1003 bp
  20% of sequence in fragments shorter than    1213 bp
  25% of sequence in fragments shorter than    1434 bp
  30% of sequence in fragments shorter than    1650 bp
  35% of sequence in fragments shorter than    1883 bp
  40% of sequence in fragments shorter than    2107 bp
  45% of sequence in fragments shorter than    2359 bp
  50% of sequence in fragments shorter than    2633 bp
  55% of sequence in fragments shorter than    2924 bp
  60% of sequence in fragments shorter than    3249 bp
  65% of sequence in fragments shorter than    3604 bp
  70% of sequence in fragments shorter than    4015 bp
  75% of sequence in fragments shorter than    4491 bp
  80% of sequence in fragments shorter than    5070 bp
  85% of sequence in fragments shorter than    5819 bp
  90% of sequence in fragments shorter than    6885 bp
  95% of sequence in fragments shorter than    8750 bp

# AflIII.ScaI (ACRYGT+AGTACT) double digest, 2028 thousand fragments (1485394+543793 sites), 1468 bp average fragment length
   5% of sequence in fragments shorter than     517 bp
  10% of sequence in fragments shorter than     803 bp
  15% of sequence in fragments shorter than    1050 bp
  20% of sequence in fragments shorter than    1277 bp
  25% of sequence in fragments shorter than    1502 bp
  30% of sequence in fragments shorter than    1726 bp
  35% of sequence in fragments shorter than    1950 bp
  40% of sequence in fragments shorter than    2179 bp
  45% of sequence in fragments shorter than    2423 bp
  50% of sequence in fragments shorter than    2683 bp
  55% of sequence in fragments shorter than    2954 bp
  60% of sequence in fragments shorter than    3257 bp
  65% of sequence in fragments shorter than    3597 bp
  70% of sequence in fragments shorter than    3984 bp
  75% of sequence in fragments shorter than    4445 bp
  80% of sequence in fragments shorter than    5005 bp
  85% of sequence in fragments shorter than    5729 bp
  90% of sequence in fragments shorter than    6723 bp
  95% of sequence in fragments shorter than    8557 bp

# AccI.StuI (GTMKAC+AGGCCT) double digest, 2026 thousand fragments (1101063+925729 sites), 1470 bp average fragment length
   5% of sequence in fragments shorter than     496 bp
  10% of sequence in fragments shorter than     750 bp
  15% of sequence in fragments shorter than     997 bp
  20% of sequence in fragments shorter than    1226 bp
  25% of sequence in fragments shorter than    1440 bp
  30% of sequence in fragments shorter than    1662 bp
  35% of sequence in fragments shorter than    1888 bp
  40% of sequence in fragments shorter than    2109 bp
  45% of sequence in fragments shorter than    2337 bp
  50% of sequence in fragments shorter than    2593 bp
  55% of sequence in fragments shorter than    2866 bp
  60% of sequence in fragments shorter than    3161 bp
  65% of sequence in fragments shorter than    3486 bp
  70% of sequence in fragments shorter than    3853 bp
  75% of sequence in fragments shorter than    4255 bp
  80% of sequence in fragments shorter than    4752 bp
  85% of sequence in fragments shorter than    5385 bp
  90% of sequence in fragments shorter than    6255 bp
  95% of sequence in fragments shorter than    7711 bp

# BceAI.PstI (ACGGC+CTGCAG) double digest, 2026 thousand fragments (705178+1321469 sites), 1470 bp average fragment length
   5% of sequence in fragments shorter than     479 bp
  10% of sequence in fragments shorter than     760 bp
  15% of sequence in fragments shorter than    1022 bp
  20% of sequence in fragments shorter than    1279 bp
  25% of sequence in fragments shorter than    1537 bp
  30% of sequence in fragments shorter than    1804 bp
  35% of sequence in fragments shorter than    2081 bp
  40% of sequence in fragments shorter than    2370 bp
  45% of sequence in fragments shorter than    2684 bp
  50% of sequence in fragments shorter than    3016 bp
  55% of sequence in fragments shorter than    3386 bp
  60% of sequence in fragments shorter than    3794 bp
  65% of sequence in fragments shorter than    4250 bp
  70% of sequence in fragments shorter than    4777 bp
  75% of sequence in fragments shorter than    5387 bp
  80% of sequence in fragments shorter than    6126 bp
  85% of sequence in fragments shorter than    7081 bp
  90% of sequence in fragments shorter than    8447 bp
  95% of sequence in fragments shorter than   10922 bp

# PsiI.SapI (TTATAA+GCTCTTC) double digest, 2024 thousand fragments (1647912+377161 sites), 1471 bp average fragment length
   5% of sequence in fragments shorter than     501 bp
  10% of sequence in fragments shorter than     775 bp
  15% of sequence in fragments shorter than    1011 bp
  20% of sequence in fragments shorter than    1238 bp
  25% of sequence in fragments shorter than    1461 bp
  30% of sequence in fragments shorter than    1690 bp
  35% of sequence in fragments shorter than    1922 bp
  40% of sequence in fragments shorter than    2163 bp
  45% of sequence in fragments shorter than    2421 bp
  50% of sequence in fragments shorter than    2701 bp
  55% of sequence in fragments shorter than    2993 bp
  60% of sequence in fragments shorter than    3320 bp
  65% of sequence in fragments shorter than    3697 bp
  70% of sequence in fragments shorter than    4132 bp
  75% of sequence in fragments shorter than    4658 bp
  80% of sequence in fragments shorter than    5326 bp
  85% of sequence in fragments shorter than    6227 bp
  90% of sequence in fragments shorter than    7642 bp
  95% of sequence in fragments shorter than   10783 bp

# PsiI.SmaI (TTATAA+CCCGGG) double digest, 2024 thousand fragments (1647912+376940 sites), 1471 bp average fragment length
   5% of sequence in fragments shorter than     501 bp
  10% of sequence in fragments shorter than     773 bp
  15% of sequence in fragments shorter than    1013 bp
  20% of sequence in fragments shorter than    1240 bp
  25% of sequence in fragments shorter than    1462 bp
  30% of sequence in fragments shorter than    1689 bp
  35% of sequence in fragments shorter than    1920 bp
  40% of sequence in fragments shorter than    2158 bp
  45% of sequence in fragments shorter than    2413 bp
  50% of sequence in fragments shorter than    2684 bp
  55% of sequence in fragments shorter than    2960 bp
  60% of sequence in fragments shorter than    3276 bp
  65% of sequence in fragments shorter than    3632 bp
  70% of sequence in fragments shorter than    4034 bp
  75% of sequence in fragments shorter than    4515 bp
  80% of sequence in fragments shorter than    5114 bp
  85% of sequence in fragments shorter than    5898 bp
  90% of sequence in fragments shorter than    7055 bp
  95% of sequence in fragments shorter than    9355 bp

# AvaIII.PpuMI (ATGCAT+RGGWCCY) double digest, 2018 thousand fragments (933357+1085138 sites), 1476 bp average fragment length
   5% of sequence in fragments shorter than     507 bp
  10% of sequence in fragments shorter than     784 bp
  15% of sequence in fragments shorter than    1027 bp
  20% of sequence in fragments shorter than    1256 bp
  25% of sequence in fragments shorter than    1482 bp
  30% of sequence in fragments shorter than    1707 bp
  35% of sequence in fragments shorter than    1933 bp
  40% of sequence in fragments shorter than    2163 bp
  45% of sequence in fragments shorter than    2412 bp
  50% of sequence in fragments shorter than    2675 bp
  55% of sequence in fragments shorter than    2960 bp
  60% of sequence in fragments shorter than    3266 bp
  65% of sequence in fragments shorter than    3590 bp
  70% of sequence in fragments shorter than    3971 bp
  75% of sequence in fragments shorter than    4430 bp
  80% of sequence in fragments shorter than    4988 bp
  85% of sequence in fragments shorter than    5711 bp
  90% of sequence in fragments shorter than    6769 bp
  95% of sequence in fragments shorter than    8822 bp

# AvaIII.PvuII (ATGCAT+CAGCTG) double digest, 2017 thousand fragments (933357+1084260 sites), 1477 bp average fragment length
   5% of sequence in fragments shorter than     513 bp
  10% of sequence in fragments shorter than     786 bp
  15% of sequence in fragments shorter than    1021 bp
  20% of sequence in fragments shorter than    1242 bp
  25% of sequence in fragments shorter than    1457 bp
  30% of sequence in fragments shorter than    1675 bp
  35% of sequence in fragments shorter than    1898 bp
  40% of sequence in fragments shorter than    2122 bp
  45% of sequence in fragments shorter than    2366 bp
  50% of sequence in fragments shorter than    2625 bp
  55% of sequence in fragments shorter than    2903 bp
  60% of sequence in fragments shorter than    3206 bp
  65% of sequence in fragments shorter than    3542 bp
  70% of sequence in fragments shorter than    3930 bp
  75% of sequence in fragments shorter than    4385 bp
  80% of sequence in fragments shorter than    4934 bp
  85% of sequence in fragments shorter than    5632 bp
  90% of sequence in fragments shorter than    6620 bp
  95% of sequence in fragments shorter than    8476 bp

# BciVI.PstI (GTATCC+CTGCAG) double digest, 2017 thousand fragments (696093+1321469 sites), 1477 bp average fragment length
   5% of sequence in fragments shorter than     509 bp
  10% of sequence in fragments shorter than     775 bp
  15% of sequence in fragments shorter than    1013 bp
  20% of sequence in fragments shorter than    1235 bp
  25% of sequence in fragments shorter than    1452 bp
  30% of sequence in fragments shorter than    1676 bp
  35% of sequence in fragments shorter than    1892 bp
  40% of sequence in fragments shorter than    2123 bp
  45% of sequence in fragments shorter than    2368 bp
  50% of sequence in fragments shorter than    2626 bp
  55% of sequence in fragments shorter than    2899 bp
  60% of sequence in fragments shorter than    3196 bp
  65% of sequence in fragments shorter than    3523 bp
  70% of sequence in fragments shorter than    3905 bp
  75% of sequence in fragments shorter than    4352 bp
  80% of sequence in fragments shorter than    4896 bp
  85% of sequence in fragments shorter than    5591 bp
  90% of sequence in fragments shorter than    6575 bp
  95% of sequence in fragments shorter than    8369 bp

# BamHI.PsiI (GGATCC+TTATAA) double digest, 2013 thousand fragments (365999+1647912 sites), 1480 bp average fragment length
   5% of sequence in fragments shorter than     510 bp
  10% of sequence in fragments shorter than     776 bp
  15% of sequence in fragments shorter than    1019 bp
  20% of sequence in fragments shorter than    1248 bp
  25% of sequence in fragments shorter than    1469 bp
  30% of sequence in fragments shorter than    1696 bp
  35% of sequence in fragments shorter than    1928 bp
  40% of sequence in fragments shorter than    2164 bp
  45% of sequence in fragments shorter than    2403 bp
  50% of sequence in fragments shorter than    2678 bp
  55% of sequence in fragments shorter than    2967 bp
  60% of sequence in fragments shorter than    3292 bp
  65% of sequence in fragments shorter than    3664 bp
  70% of sequence in fragments shorter than    4089 bp
  75% of sequence in fragments shorter than    4598 bp
  80% of sequence in fragments shorter than    5233 bp
  85% of sequence in fragments shorter than    6068 bp
  90% of sequence in fragments shorter than    7322 bp
  95% of sequence in fragments shorter than    9913 bp

# FauI.HgaI (CCCGC+GACGC) double digest, 2011 thousand fragments (1439772+571889 sites), 1481 bp average fragment length
   5% of sequence in fragments shorter than     496 bp
  10% of sequence in fragments shorter than     887 bp
  15% of sequence in fragments shorter than    1265 bp
  20% of sequence in fragments shorter than    1630 bp
  25% of sequence in fragments shorter than    2017 bp
  30% of sequence in fragments shorter than    2422 bp
  35% of sequence in fragments shorter than    2841 bp
  40% of sequence in fragments shorter than    3298 bp
  45% of sequence in fragments shorter than    3802 bp
  50% of sequence in fragments shorter than    4342 bp
  55% of sequence in fragments shorter than    4947 bp
  60% of sequence in fragments shorter than    5623 bp
  65% of sequence in fragments shorter than    6386 bp
  70% of sequence in fragments shorter than    7262 bp
  75% of sequence in fragments shorter than    8307 bp
  80% of sequence in fragments shorter than    9611 bp
  85% of sequence in fragments shorter than   11287 bp
  90% of sequence in fragments shorter than   13741 bp
  95% of sequence in fragments shorter than   18252 bp

# ApaI.HgiCI (GGGCCC+GGYRCC) double digest, 2010 thousand fragments (460339+1550877 sites), 1481 bp average fragment length
   5% of sequence in fragments shorter than     471 bp
  10% of sequence in fragments shorter than     760 bp
  15% of sequence in fragments shorter than    1035 bp
  20% of sequence in fragments shorter than    1276 bp
  25% of sequence in fragments shorter than    1543 bp
  30% of sequence in fragments shorter than    1806 bp
  35% of sequence in fragments shorter than    2095 bp
  40% of sequence in fragments shorter than    2404 bp
  45% of sequence in fragments shorter than    2728 bp
  50% of sequence in fragments shorter than    3059 bp
  55% of sequence in fragments shorter than    3440 bp
  60% of sequence in fragments shorter than    3869 bp
  65% of sequence in fragments shorter than    4348 bp
  70% of sequence in fragments shorter than    4909 bp
  75% of sequence in fragments shorter than    5576 bp
  80% of sequence in fragments shorter than    6407 bp
  85% of sequence in fragments shorter than    7515 bp
  90% of sequence in fragments shorter than    9175 bp
  95% of sequence in fragments shorter than   12595 bp

# EciI.FauI (GGCGGA+CCCGC) double digest, 2010 thousand fragments (571273+1439772 sites), 1482 bp average fragment length
   5% of sequence in fragments shorter than     500 bp
  10% of sequence in fragments shorter than     904 bp
  15% of sequence in fragments shorter than    1290 bp
  20% of sequence in fragments shorter than    1674 bp
  25% of sequence in fragments shorter than    2085 bp
  30% of sequence in fragments shorter than    2518 bp
  35% of sequence in fragments shorter than    2984 bp
  40% of sequence in fragments shorter than    3497 bp
  45% of sequence in fragments shorter than    4057 bp
  50% of sequence in fragments shorter than    4653 bp
  55% of sequence in fragments shorter than    5346 bp
  60% of sequence in fragments shorter than    6121 bp
  65% of sequence in fragments shorter than    7014 bp
  70% of sequence in fragments shorter than    8048 bp
  75% of sequence in fragments shorter than    9276 bp
  80% of sequence in fragments shorter than   10827 bp
  85% of sequence in fragments shorter than   12854 bp
  90% of sequence in fragments shorter than   15832 bp
  95% of sequence in fragments shorter than   21473 bp

# PpuMI.StuI (RGGWCCY+AGGCCT) double digest, 2010 thousand fragments (1085138+925729 sites), 1482 bp average fragment length
   5% of sequence in fragments shorter than     453 bp
  10% of sequence in fragments shorter than     719 bp
  15% of sequence in fragments shorter than    1007 bp
  20% of sequence in fragments shorter than    1277 bp
  25% of sequence in fragments shorter than    1552 bp
  30% of sequence in fragments shorter than    1843 bp
  35% of sequence in fragments shorter than    2122 bp
  40% of sequence in fragments shorter than    2438 bp
  45% of sequence in fragments shorter than    2761 bp
  50% of sequence in fragments shorter than    3114 bp
  55% of sequence in fragments shorter than    3500 bp
  60% of sequence in fragments shorter than    3920 bp
  65% of sequence in fragments shorter than    4395 bp
  70% of sequence in fragments shorter than    4940 bp
  75% of sequence in fragments shorter than    5571 bp
  80% of sequence in fragments shorter than    6339 bp
  85% of sequence in fragments shorter than    7313 bp
  90% of sequence in fragments shorter than    8674 bp
  95% of sequence in fragments shorter than   11003 bp

# BspMI.HgaI (ACCTGC+GACGC) double digest, 2009 thousand fragments (1438129+571889 sites), 1482 bp average fragment length
   5% of sequence in fragments shorter than     481 bp
  10% of sequence in fragments shorter than     774 bp
  15% of sequence in fragments shorter than    1029 bp
  20% of sequence in fragments shorter than    1288 bp
  25% of sequence in fragments shorter than    1543 bp
  30% of sequence in fragments shorter than    1807 bp
  35% of sequence in fragments shorter than    2058 bp
  40% of sequence in fragments shorter than    2336 bp
  45% of sequence in fragments shorter than    2635 bp
  50% of sequence in fragments shorter than    2936 bp
  55% of sequence in fragments shorter than    3267 bp
  60% of sequence in fragments shorter than    3626 bp
  65% of sequence in fragments shorter than    4048 bp
  70% of sequence in fragments shorter than    4534 bp
  75% of sequence in fragments shorter than    5095 bp
  80% of sequence in fragments shorter than    5774 bp
  85% of sequence in fragments shorter than    6649 bp
  90% of sequence in fragments shorter than    7895 bp
  95% of sequence in fragments shorter than   10030 bp

# PvuII.StuI (CAGCTG+AGGCCT) double digest, 2009 thousand fragments (1084260+925729 sites), 1482 bp average fragment length
   5% of sequence in fragments shorter than     469 bp
  10% of sequence in fragments shorter than     733 bp
  15% of sequence in fragments shorter than    1003 bp
  20% of sequence in fragments shorter than    1253 bp
  25% of sequence in fragments shorter than    1508 bp
  30% of sequence in fragments shorter than    1773 bp
  35% of sequence in fragments shorter than    2035 bp
  40% of sequence in fragments shorter than    2325 bp
  45% of sequence in fragments shorter than    2628 bp
  50% of sequence in fragments shorter than    2949 bp
  55% of sequence in fragments shorter than    3299 bp
  60% of sequence in fragments shorter than    3683 bp
  65% of sequence in fragments shorter than    4118 bp
  70% of sequence in fragments shorter than    4604 bp
  75% of sequence in fragments shorter than    5177 bp
  80% of sequence in fragments shorter than    5873 bp
  85% of sequence in fragments shorter than    6752 bp
  90% of sequence in fragments shorter than    7984 bp
  95% of sequence in fragments shorter than   10084 bp

# ApaI.BsmI (GGGCCC+GAATGC) double digest, 2009 thousand fragments (460339+1549350 sites), 1483 bp average fragment length
   5% of sequence in fragments shorter than     481 bp
  10% of sequence in fragments shorter than     838 bp
  15% of sequence in fragments shorter than    1131 bp
  20% of sequence in fragments shorter than    1411 bp
  25% of sequence in fragments shorter than    1684 bp
  30% of sequence in fragments shorter than    1954 bp
  35% of sequence in fragments shorter than    2228 bp
  40% of sequence in fragments shorter than    2503 bp
  45% of sequence in fragments shorter than    2789 bp
  50% of sequence in fragments shorter than    3098 bp
  55% of sequence in fragments shorter than    3429 bp
  60% of sequence in fragments shorter than    3789 bp
  65% of sequence in fragments shorter than    4187 bp
  70% of sequence in fragments shorter than    4642 bp
  75% of sequence in fragments shorter than    5166 bp
  80% of sequence in fragments shorter than    5801 bp
  85% of sequence in fragments shorter than    6595 bp
  90% of sequence in fragments shorter than    7695 bp
  95% of sequence in fragments shorter than    9591 bp

# BspMI.EciI (ACCTGC+GGCGGA) double digest, 2008 thousand fragments (1438129+571273 sites), 1483 bp average fragment length
   5% of sequence in fragments shorter than     483 bp
  10% of sequence in fragments shorter than     765 bp
  15% of sequence in fragments shorter than    1011 bp
  20% of sequence in fragments shorter than    1255 bp
  25% of sequence in fragments shorter than    1493 bp
  30% of sequence in fragments shorter than    1743 bp
  35% of sequence in fragments shorter than    1988 bp
  40% of sequence in fragments shorter than    2251 bp
  45% of sequence in fragments shorter than    2539 bp
  50% of sequence in fragments shorter than    2842 bp
  55% of sequence in fragments shorter than    3178 bp
  60% of sequence in fragments shorter than    3495 bp
  65% of sequence in fragments shorter than    3908 bp
  70% of sequence in fragments shorter than    4380 bp
  75% of sequence in fragments shorter than    4936 bp
  80% of sequence in fragments shorter than    5605 bp
  85% of sequence in fragments shorter than    6470 bp
  90% of sequence in fragments shorter than    7701 bp
  95% of sequence in fragments shorter than    9835 bp

# BglII.BscGI (AGATCT+CCCGT) double digest, 2005 thousand fragments (774732+1231070 sites), 1486 bp average fragment length
   5% of sequence in fragments shorter than     462 bp
  10% of sequence in fragments shorter than     746 bp
  15% of sequence in fragments shorter than    1003 bp
  20% of sequence in fragments shorter than    1247 bp
  25% of sequence in fragments shorter than    1489 bp
  30% of sequence in fragments shorter than    1730 bp
  35% of sequence in fragments shorter than    1978 bp
  40% of sequence in fragments shorter than    2236 bp
  45% of sequence in fragments shorter than    2508 bp
  50% of sequence in fragments shorter than    2797 bp
  55% of sequence in fragments shorter than    3109 bp
  60% of sequence in fragments shorter than    3450 bp
  65% of sequence in fragments shorter than    3825 bp
  70% of sequence in fragments shorter than    4252 bp
  75% of sequence in fragments shorter than    4738 bp
  80% of sequence in fragments shorter than    5331 bp
  85% of sequence in fragments shorter than    6086 bp
  90% of sequence in fragments shorter than    7132 bp
  95% of sequence in fragments shorter than    8959 bp

# BfiI.SexAI (ACTGGG+ACCWGGT) double digest, 2004 thousand fragments (1706594+297900 sites), 1486 bp average fragment length
   5% of sequence in fragments shorter than     498 bp
  10% of sequence in fragments shorter than     775 bp
  15% of sequence in fragments shorter than    1018 bp
  20% of sequence in fragments shorter than    1247 bp
  25% of sequence in fragments shorter than    1476 bp
  30% of sequence in fragments shorter than    1705 bp
  35% of sequence in fragments shorter than    1936 bp
  40% of sequence in fragments shorter than    2171 bp
  45% of sequence in fragments shorter than    2418 bp
  50% of sequence in fragments shorter than    2691 bp
  55% of sequence in fragments shorter than    2957 bp
  60% of sequence in fragments shorter than    3277 bp
  65% of sequence in fragments shorter than    3638 bp
  70% of sequence in fragments shorter than    4052 bp
  75% of sequence in fragments shorter than    4530 bp
  80% of sequence in fragments shorter than    5110 bp
  85% of sequence in fragments shorter than    5863 bp
  90% of sequence in fragments shorter than    6978 bp
  95% of sequence in fragments shorter than    9139 bp

# AccI.NdeI (GTMKAC+CATATG) double digest, 2003 thousand fragments (1101063+903360 sites), 1487 bp average fragment length
   5% of sequence in fragments shorter than     517 bp
  10% of sequence in fragments shorter than     795 bp
  15% of sequence in fragments shorter than    1038 bp
  20% of sequence in fragments shorter than    1249 bp
  25% of sequence in fragments shorter than    1471 bp
  30% of sequence in fragments shorter than    1700 bp
  35% of sequence in fragments shorter than    1930 bp
  40% of sequence in fragments shorter than    2173 bp
  45% of sequence in fragments shorter than    2426 bp
  50% of sequence in fragments shorter than    2695 bp
  55% of sequence in fragments shorter than    2984 bp
  60% of sequence in fragments shorter than    3310 bp
  65% of sequence in fragments shorter than    3666 bp
  70% of sequence in fragments shorter than    4074 bp
  75% of sequence in fragments shorter than    4545 bp
  80% of sequence in fragments shorter than    5104 bp
  85% of sequence in fragments shorter than    5876 bp
  90% of sequence in fragments shorter than    7016 bp
  95% of sequence in fragments shorter than    9216 bp

# FauI.MfeI (CCCGC+CAATTG) double digest, 2003 thousand fragments (1439772+564303 sites), 1487 bp average fragment length
   5% of sequence in fragments shorter than     525 bp
  10% of sequence in fragments shorter than     869 bp
  15% of sequence in fragments shorter than    1177 bp
  20% of sequence in fragments shorter than    1466 bp
  25% of sequence in fragments shorter than    1756 bp
  30% of sequence in fragments shorter than    2050 bp
  35% of sequence in fragments shorter than    2344 bp
  40% of sequence in fragments shorter than    2659 bp
  45% of sequence in fragments shorter than    2989 bp
  50% of sequence in fragments shorter than    3340 bp
  55% of sequence in fragments shorter than    3726 bp
  60% of sequence in fragments shorter than    4114 bp
  65% of sequence in fragments shorter than    4568 bp
  70% of sequence in fragments shorter than    5092 bp
  75% of sequence in fragments shorter than    5702 bp
  80% of sequence in fragments shorter than    6440 bp
  85% of sequence in fragments shorter than    7398 bp
  90% of sequence in fragments shorter than    8733 bp
  95% of sequence in fragments shorter than   11140 bp

# EsaBC3I.SpeI (TCGA+ACTAGT) double digest, 2003 thousand fragments (1603346+400286 sites), 1487 bp average fragment length
   5% of sequence in fragments shorter than     517 bp
  10% of sequence in fragments shorter than     786 bp
  15% of sequence in fragments shorter than    1018 bp
  20% of sequence in fragments shorter than    1235 bp
  25% of sequence in fragments shorter than    1442 bp
  30% of sequence in fragments shorter than    1660 bp
  35% of sequence in fragments shorter than    1877 bp
  40% of sequence in fragments shorter than    2102 bp
  45% of sequence in fragments shorter than    2336 bp
  50% of sequence in fragments shorter than    2590 bp
  55% of sequence in fragments shorter than    2860 bp
  60% of sequence in fragments shorter than    3155 bp
  65% of sequence in fragments shorter than    3484 bp
  70% of sequence in fragments shorter than    3849 bp
  75% of sequence in fragments shorter than    4273 bp
  80% of sequence in fragments shorter than    4799 bp
  85% of sequence in fragments shorter than    5463 bp
  90% of sequence in fragments shorter than    6387 bp
  95% of sequence in fragments shorter than    7986 bp

# BspMI.MfeI (ACCTGC+CAATTG) double digest, 2001 thousand fragments (1438129+564303 sites), 1488 bp average fragment length
   5% of sequence in fragments shorter than     518 bp
  10% of sequence in fragments shorter than     794 bp
  15% of sequence in fragments shorter than    1023 bp
  20% of sequence in fragments shorter than    1246 bp
  25% of sequence in fragments shorter than    1460 bp
  30% of sequence in fragments shorter than    1667 bp
  35% of sequence in fragments shorter than    1868 bp
  40% of sequence in fragments shorter than    2079 bp
  45% of sequence in fragments shorter than    2317 bp
  50% of sequence in fragments shorter than    2573 bp
  55% of sequence in fragments shorter than    2841 bp
  60% of sequence in fragments shorter than    3135 bp
  65% of sequence in fragments shorter than    3457 bp
  70% of sequence in fragments shorter than    3815 bp
  75% of sequence in fragments shorter than    4239 bp
  80% of sequence in fragments shorter than    4750 bp
  85% of sequence in fragments shorter than    5398 bp
  90% of sequence in fragments shorter than    6294 bp
  95% of sequence in fragments shorter than    7847 bp

# EcoRV.HgiCI (GATATC+GGYRCC) double digest, 1996 thousand fragments (446473+1550877 sites), 1492 bp average fragment length
   5% of sequence in fragments shorter than     504 bp
  10% of sequence in fragments shorter than     767 bp
  15% of sequence in fragments shorter than    1015 bp
  20% of sequence in fragments shorter than    1229 bp
  25% of sequence in fragments shorter than    1449 bp
  30% of sequence in fragments shorter than    1677 bp
  35% of sequence in fragments shorter than    1908 bp
  40% of sequence in fragments shorter than    2152 bp
  45% of sequence in fragments shorter than    2409 bp
  50% of sequence in fragments shorter than    2677 bp
  55% of sequence in fragments shorter than    2968 bp
  60% of sequence in fragments shorter than    3290 bp
  65% of sequence in fragments shorter than    3651 bp
  70% of sequence in fragments shorter than    4063 bp
  75% of sequence in fragments shorter than    4545 bp
  80% of sequence in fragments shorter than    5132 bp
  85% of sequence in fragments shorter than    5886 bp
  90% of sequence in fragments shorter than    6975 bp
  95% of sequence in fragments shorter than    9058 bp

# BsmI.EcoRV (GAATGC+GATATC) double digest, 1995 thousand fragments (1549350+446473 sites), 1493 bp average fragment length
   5% of sequence in fragments shorter than     505 bp
  10% of sequence in fragments shorter than     848 bp
  15% of sequence in fragments shorter than    1136 bp
  20% of sequence in fragments shorter than    1407 bp
  25% of sequence in fragments shorter than    1668 bp
  30% of sequence in fragments shorter than    1925 bp
  35% of sequence in fragments shorter than    2181 bp
  40% of sequence in fragments shorter than    2446 bp
  45% of sequence in fragments shorter than    2723 bp
  50% of sequence in fragments shorter than    3016 bp
  55% of sequence in fragments shorter than    3330 bp
  60% of sequence in fragments shorter than    3671 bp
  65% of sequence in fragments shorter than    4050 bp
  70% of sequence in fragments shorter than    4474 bp
  75% of sequence in fragments shorter than    4968 bp
  80% of sequence in fragments shorter than    5562 bp
  85% of sequence in fragments shorter than    6315 bp
  90% of sequence in fragments shorter than    7350 bp
  95% of sequence in fragments shorter than    9155 bp

# BfiI.KpnI (ACTGGG+GGTACC) double digest, 1994 thousand fragments (1706594+287974 sites), 1494 bp average fragment length
   5% of sequence in fragments shorter than     512 bp
  10% of sequence in fragments shorter than     785 bp
  15% of sequence in fragments shorter than    1010 bp
  20% of sequence in fragments shorter than    1225 bp
  25% of sequence in fragments shorter than    1451 bp
  30% of sequence in fragments shorter than    1682 bp
  35% of sequence in fragments shorter than    1914 bp
  40% of sequence in fragments shorter than    2149 bp
  45% of sequence in fragments shorter than    2403 bp
  50% of sequence in fragments shorter than    2677 bp
  55% of sequence in fragments shorter than    2975 bp
  60% of sequence in fragments shorter than    3301 bp
  65% of sequence in fragments shorter than    3664 bp
  70% of sequence in fragments shorter than    4082 bp
  75% of sequence in fragments shorter than    4566 bp
  80% of sequence in fragments shorter than    5165 bp
  85% of sequence in fragments shorter than    5934 bp
  90% of sequence in fragments shorter than    7069 bp
  95% of sequence in fragments shorter than    9279 bp

# BfiI.NheI (ACTGGG+GCTAGC) double digest, 1993 thousand fragments (1706594+287384 sites), 1494 bp average fragment length
   5% of sequence in fragments shorter than     510 bp
  10% of sequence in fragments shorter than     775 bp
  15% of sequence in fragments shorter than    1022 bp
  20% of sequence in fragments shorter than    1252 bp
  25% of sequence in fragments shorter than    1479 bp
  30% of sequence in fragments shorter than    1708 bp
  35% of sequence in fragments shorter than    1917 bp
  40% of sequence in fragments shorter than    2147 bp
  45% of sequence in fragments shorter than    2392 bp
  50% of sequence in fragments shorter than    2655 bp
  55% of sequence in fragments shorter than    2950 bp
  60% of sequence in fragments shorter than    3272 bp
  65% of sequence in fragments shorter than    3630 bp
  70% of sequence in fragments shorter than    4041 bp
  75% of sequence in fragments shorter than    4518 bp
  80% of sequence in fragments shorter than    5103 bp
  85% of sequence in fragments shorter than    5850 bp
  90% of sequence in fragments shorter than    6937 bp
  95% of sequence in fragments shorter than    9007 bp

# EsaBC3I.HpaI (TCGA+GTTAAC) double digest, 1992 thousand fragments (1603346+389418 sites), 1495 bp average fragment length
   5% of sequence in fragments shorter than     514 bp
  10% of sequence in fragments shorter than     784 bp
  15% of sequence in fragments shorter than    1017 bp
  20% of sequence in fragments shorter than    1239 bp
  25% of sequence in fragments shorter than    1450 bp
  30% of sequence in fragments shorter than    1674 bp
  35% of sequence in fragments shorter than    1900 bp
  40% of sequence in fragments shorter than    2136 bp
  45% of sequence in fragments shorter than    2383 bp
  50% of sequence in fragments shorter than    2646 bp
  55% of sequence in fragments shorter than    2928 bp
  60% of sequence in fragments shorter than    3229 bp
  65% of sequence in fragments shorter than    3568 bp
  70% of sequence in fragments shorter than    3955 bp
  75% of sequence in fragments shorter than    4402 bp
  80% of sequence in fragments shorter than    4938 bp
  85% of sequence in fragments shorter than    5627 bp
  90% of sequence in fragments shorter than    6595 bp
  95% of sequence in fragments shorter than    8287 bp

# BsrBI.HhaI (CCGCTC+GCGC) double digest, 1991 thousand fragments (235523+1756502 sites), 1496 bp average fragment length
   5% of sequence in fragments shorter than     499 bp
  10% of sequence in fragments shorter than     873 bp
  15% of sequence in fragments shorter than    1260 bp
  20% of sequence in fragments shorter than    1657 bp
  25% of sequence in fragments shorter than    2055 bp
  30% of sequence in fragments shorter than    2486 bp
  35% of sequence in fragments shorter than    2950 bp
  40% of sequence in fragments shorter than    3441 bp
  45% of sequence in fragments shorter than    3981 bp
  50% of sequence in fragments shorter than    4582 bp
  55% of sequence in fragments shorter than    5247 bp
  60% of sequence in fragments shorter than    5992 bp
  65% of sequence in fragments shorter than    6829 bp
  70% of sequence in fragments shorter than    7803 bp
  75% of sequence in fragments shorter than    8961 bp
  80% of sequence in fragments shorter than   10400 bp
  85% of sequence in fragments shorter than   12257 bp
  90% of sequence in fragments shorter than   14922 bp
  95% of sequence in fragments shorter than   19491 bp

# FauI.SphI (CCCGC+GCATGC) double digest, 1990 thousand fragments (1439772+550952 sites), 1497 bp average fragment length
   5% of sequence in fragments shorter than     510 bp
  10% of sequence in fragments shorter than     863 bp
  15% of sequence in fragments shorter than    1189 bp
  20% of sequence in fragments shorter than    1499 bp
  25% of sequence in fragments shorter than    1823 bp
  30% of sequence in fragments shorter than    2151 bp
  35% of sequence in fragments shorter than    2506 bp
  40% of sequence in fragments shorter than    2881 bp
  45% of sequence in fragments shorter than    3281 bp
  50% of sequence in fragments shorter than    3720 bp
  55% of sequence in fragments shorter than    4180 bp
  60% of sequence in fragments shorter than    4709 bp
  65% of sequence in fragments shorter than    5317 bp
  70% of sequence in fragments shorter than    6011 bp
  75% of sequence in fragments shorter than    6829 bp
  80% of sequence in fragments shorter than    7832 bp
  85% of sequence in fragments shorter than    9138 bp
  90% of sequence in fragments shorter than   11042 bp
  95% of sequence in fragments shorter than   14564 bp

# BscGI.NcoI (CCCGT+CCATGG) double digest, 1990 thousand fragments (1231070+759594 sites), 1497 bp average fragment length
   5% of sequence in fragments shorter than     445 bp
  10% of sequence in fragments shorter than     740 bp
  15% of sequence in fragments shorter than    1016 bp
  20% of sequence in fragments shorter than    1280 bp
  25% of sequence in fragments shorter than    1548 bp
  30% of sequence in fragments shorter than    1818 bp
  35% of sequence in fragments shorter than    2099 bp
  40% of sequence in fragments shorter than    2393 bp
  45% of sequence in fragments shorter than    2703 bp
  50% of sequence in fragments shorter than    3038 bp
  55% of sequence in fragments shorter than    3398 bp
  60% of sequence in fragments shorter than    3789 bp
  65% of sequence in fragments shorter than    4223 bp
  70% of sequence in fragments shorter than    4682 bp
  75% of sequence in fragments shorter than    5249 bp
  80% of sequence in fragments shorter than    5945 bp
  85% of sequence in fragments shorter than    6847 bp
  90% of sequence in fragments shorter than    8115 bp
  95% of sequence in fragments shorter than   10280 bp

# BspMI.SphI (ACCTGC+GCATGC) double digest, 1988 thousand fragments (1438129+550952 sites), 1498 bp average fragment length
   5% of sequence in fragments shorter than     506 bp
  10% of sequence in fragments shorter than     787 bp
  15% of sequence in fragments shorter than    1026 bp
  20% of sequence in fragments shorter than    1261 bp
  25% of sequence in fragments shorter than    1487 bp
  30% of sequence in fragments shorter than    1722 bp
  35% of sequence in fragments shorter than    1964 bp
  40% of sequence in fragments shorter than    2194 bp
  45% of sequence in fragments shorter than    2457 bp
  50% of sequence in fragments shorter than    2739 bp
  55% of sequence in fragments shorter than    3037 bp
  60% of sequence in fragments shorter than    3326 bp
  65% of sequence in fragments shorter than    3689 bp
  70% of sequence in fragments shorter than    4106 bp
  75% of sequence in fragments shorter than    4592 bp
  80% of sequence in fragments shorter than    5179 bp
  85% of sequence in fragments shorter than    5919 bp
  90% of sequence in fragments shorter than    6982 bp
  95% of sequence in fragments shorter than    8838 bp

# NdeI.PpuMI (CATATG+RGGWCCY) double digest, 1988 thousand fragments (903360+1085138 sites), 1498 bp average fragment length
   5% of sequence in fragments shorter than     503 bp
  10% of sequence in fragments shorter than     786 bp
  15% of sequence in fragments shorter than    1034 bp
  20% of sequence in fragments shorter than    1253 bp
  25% of sequence in fragments shorter than    1483 bp
  30% of sequence in fragments shorter than    1714 bp
  35% of sequence in fragments shorter than    1948 bp
  40% of sequence in fragments shorter than    2190 bp
  45% of sequence in fragments shorter than    2448 bp
  50% of sequence in fragments shorter than    2719 bp
  55% of sequence in fragments shorter than    3009 bp
  60% of sequence in fragments shorter than    3323 bp
  65% of sequence in fragments shorter than    3653 bp
  70% of sequence in fragments shorter than    4049 bp
  75% of sequence in fragments shorter than    4518 bp
  80% of sequence in fragments shorter than    5102 bp
  85% of sequence in fragments shorter than    5862 bp
  90% of sequence in fragments shorter than    6992 bp
  95% of sequence in fragments shorter than    9258 bp

# NdeI.PvuII (CATATG+CAGCTG) double digest, 1987 thousand fragments (903360+1084260 sites), 1499 bp average fragment length
   5% of sequence in fragments shorter than     519 bp
  10% of sequence in fragments shorter than     797 bp
  15% of sequence in fragments shorter than    1031 bp
  20% of sequence in fragments shorter than    1237 bp
  25% of sequence in fragments shorter than    1454 bp
  30% of sequence in fragments shorter than    1672 bp
  35% of sequence in fragments shorter than    1896 bp
  40% of sequence in fragments shorter than    2126 bp
  45% of sequence in fragments shorter than    2366 bp
  50% of sequence in fragments shorter than    2623 bp
  55% of sequence in fragments shorter than    2888 bp
  60% of sequence in fragments shorter than    3178 bp
  65% of sequence in fragments shorter than    3507 bp
  70% of sequence in fragments shorter than    3857 bp
  75% of sequence in fragments shorter than    4286 bp
  80% of sequence in fragments shorter than    4808 bp
  85% of sequence in fragments shorter than    5486 bp
  90% of sequence in fragments shorter than    6475 bp
  95% of sequence in fragments shorter than    8393 bp

# HhaI.KasI (GCGC+GGCGCC) double digest, 1986 thousand fragments (1756502+230381 sites), 1500 bp average fragment length
   5% of sequence in fragments shorter than     571 bp
  10% of sequence in fragments shorter than    1006 bp
  15% of sequence in fragments shorter than    1440 bp
  20% of sequence in fragments shorter than    1881 bp
  25% of sequence in fragments shorter than    2352 bp
  30% of sequence in fragments shorter than    2842 bp
  35% of sequence in fragments shorter than    3374 bp
  40% of sequence in fragments shorter than    3941 bp
  45% of sequence in fragments shorter than    4554 bp
  50% of sequence in fragments shorter than    5231 bp
  55% of sequence in fragments shorter than    5980 bp
  60% of sequence in fragments shorter than    6818 bp
  65% of sequence in fragments shorter than    7775 bp
  70% of sequence in fragments shorter than    8880 bp
  75% of sequence in fragments shorter than   10219 bp
  80% of sequence in fragments shorter than   11862 bp
  85% of sequence in fragments shorter than   13974 bp
  90% of sequence in fragments shorter than   17029 bp
  95% of sequence in fragments shorter than   22360 bp

# AvaI.BspHI (CYCGRG+TCATGA) double digest, 1983 thousand fragments (1005714+978290 sites), 1502 bp average fragment length
   5% of sequence in fragments shorter than     521 bp
  10% of sequence in fragments shorter than     805 bp
  15% of sequence in fragments shorter than    1057 bp
  20% of sequence in fragments shorter than    1299 bp
  25% of sequence in fragments shorter than    1537 bp
  30% of sequence in fragments shorter than    1775 bp
  35% of sequence in fragments shorter than    2012 bp
  40% of sequence in fragments shorter than    2253 bp
  45% of sequence in fragments shorter than    2513 bp
  50% of sequence in fragments shorter than    2775 bp
  55% of sequence in fragments shorter than    3071 bp
  60% of sequence in fragments shorter than    3398 bp
  65% of sequence in fragments shorter than    3763 bp
  70% of sequence in fragments shorter than    4174 bp
  75% of sequence in fragments shorter than    4660 bp
  80% of sequence in fragments shorter than    5256 bp
  85% of sequence in fragments shorter than    6033 bp
  90% of sequence in fragments shorter than    7161 bp
  95% of sequence in fragments shorter than    9382 bp

# BstEII.PsiI (GGTNACC+TTATAA) double digest, 1983 thousand fragments (335865+1647912 sites), 1502 bp average fragment length
   5% of sequence in fragments shorter than     515 bp
  10% of sequence in fragments shorter than     792 bp
  15% of sequence in fragments shorter than    1038 bp
  20% of sequence in fragments shorter than    1266 bp
  25% of sequence in fragments shorter than    1494 bp
  30% of sequence in fragments shorter than    1726 bp
  35% of sequence in fragments shorter than    1963 bp
  40% of sequence in fragments shorter than    2213 bp
  45% of sequence in fragments shorter than    2476 bp
  50% of sequence in fragments shorter than    2760 bp
  55% of sequence in fragments shorter than    3052 bp
  60% of sequence in fragments shorter than    3391 bp
  65% of sequence in fragments shorter than    3769 bp
  70% of sequence in fragments shorter than    4192 bp
  75% of sequence in fragments shorter than    4711 bp
  80% of sequence in fragments shorter than    5358 bp
  85% of sequence in fragments shorter than    6235 bp
  90% of sequence in fragments shorter than    7576 bp
  95% of sequence in fragments shorter than   10422 bp

# FauI.ScaI (CCCGC+AGTACT) double digest, 1983 thousand fragments (1439772+543793 sites), 1502 bp average fragment length
   5% of sequence in fragments shorter than     530 bp
  10% of sequence in fragments shorter than     875 bp
  15% of sequence in fragments shorter than    1189 bp
  20% of sequence in fragments shorter than    1481 bp
  25% of sequence in fragments shorter than    1778 bp
  30% of sequence in fragments shorter than    2080 bp
  35% of sequence in fragments shorter than    2390 bp
  40% of sequence in fragments shorter than    2712 bp
  45% of sequence in fragments shorter than    3060 bp
  50% of sequence in fragments shorter than    3434 bp
  55% of sequence in fragments shorter than    3842 bp
  60% of sequence in fragments shorter than    4273 bp
  65% of sequence in fragments shorter than    4770 bp
  70% of sequence in fragments shorter than    5341 bp
  75% of sequence in fragments shorter than    6020 bp
  80% of sequence in fragments shorter than    6838 bp
  85% of sequence in fragments shorter than    7898 bp
  90% of sequence in fragments shorter than    9415 bp
  95% of sequence in fragments shorter than   12291 bp

# HhaI.SwaI (GCGC+ATTTAAAT) double digest, 1982 thousand fragments (1756502+226602 sites), 1503 bp average fragment length
   5% of sequence in fragments shorter than     517 bp
  10% of sequence in fragments shorter than     876 bp
  15% of sequence in fragments shorter than    1226 bp
  20% of sequence in fragments shorter than    1575 bp
  25% of sequence in fragments shorter than    1923 bp
  30% of sequence in fragments shorter than    2290 bp
  35% of sequence in fragments shorter than    2669 bp
  40% of sequence in fragments shorter than    3070 bp
  45% of sequence in fragments shorter than    3493 bp
  50% of sequence in fragments shorter than    3957 bp
  55% of sequence in fragments shorter than    4458 bp
  60% of sequence in fragments shorter than    5017 bp
  65% of sequence in fragments shorter than    5642 bp
  70% of sequence in fragments shorter than    6358 bp
  75% of sequence in fragments shorter than    7194 bp
  80% of sequence in fragments shorter than    8225 bp
  85% of sequence in fragments shorter than    9537 bp
  90% of sequence in fragments shorter than   11406 bp
  95% of sequence in fragments shorter than   14611 bp

# BspMI.ScaI (ACCTGC+AGTACT) double digest, 1981 thousand fragments (1438129+543793 sites), 1503 bp average fragment length
   5% of sequence in fragments shorter than     518 bp
  10% of sequence in fragments shorter than     797 bp
  15% of sequence in fragments shorter than    1031 bp
  20% of sequence in fragments shorter than    1258 bp
  25% of sequence in fragments shorter than    1473 bp
  30% of sequence in fragments shorter than    1698 bp
  35% of sequence in fragments shorter than    1925 bp
  40% of sequence in fragments shorter than    2138 bp
  45% of sequence in fragments shorter than    2382 bp
  50% of sequence in fragments shorter than    2642 bp
  55% of sequence in fragments shorter than    2915 bp
  60% of sequence in fragments shorter than    3213 bp
  65% of sequence in fragments shorter than    3510 bp
  70% of sequence in fragments shorter than    3884 bp
  75% of sequence in fragments shorter than    4319 bp
  80% of sequence in fragments shorter than    4843 bp
  85% of sequence in fragments shorter than    5498 bp
  90% of sequence in fragments shorter than    6426 bp
  95% of sequence in fragments shorter than    8053 bp

# AflIII.ApaLI (ACRYGT+GTGCAC) double digest, 1981 thousand fragments (1485394+496255 sites), 1504 bp average fragment length
   5% of sequence in fragments shorter than     550 bp
  10% of sequence in fragments shorter than     853 bp
  15% of sequence in fragments shorter than    1113 bp
  20% of sequence in fragments shorter than    1354 bp
  25% of sequence in fragments shorter than    1591 bp
  30% of sequence in fragments shorter than    1827 bp
  35% of sequence in fragments shorter than    2066 bp
  40% of sequence in fragments shorter than    2306 bp
  45% of sequence in fragments shorter than    2564 bp
  50% of sequence in fragments shorter than    2832 bp
  55% of sequence in fragments shorter than    3118 bp
  60% of sequence in fragments shorter than    3437 bp
  65% of sequence in fragments shorter than    3789 bp
  70% of sequence in fragments shorter than    4186 bp
  75% of sequence in fragments shorter than    4652 bp
  80% of sequence in fragments shorter than    5218 bp
  85% of sequence in fragments shorter than    5911 bp
  90% of sequence in fragments shorter than    6892 bp
  95% of sequence in fragments shorter than    8663 bp

# EsaBC3I.SapI (TCGA+GCTCTTC) double digest, 1980 thousand fragments (1603346+377161 sites), 1505 bp average fragment length
   5% of sequence in fragments shorter than     512 bp
  10% of sequence in fragments shorter than     786 bp
  15% of sequence in fragments shorter than    1024 bp
  20% of sequence in fragments shorter than    1250 bp
  25% of sequence in fragments shorter than    1469 bp
  30% of sequence in fragments shorter than    1699 bp
  35% of sequence in fragments shorter than    1932 bp
  40% of sequence in fragments shorter than    2178 bp
  45% of sequence in fragments shorter than    2435 bp
  50% of sequence in fragments shorter than    2707 bp
  55% of sequence in fragments shorter than    2996 bp
  60% of sequence in fragments shorter than    3316 bp
  65% of sequence in fragments shorter than    3672 bp
  70% of sequence in fragments shorter than    4075 bp
  75% of sequence in fragments shorter than    4550 bp
  80% of sequence in fragments shorter than    5119 bp
  85% of sequence in fragments shorter than    5857 bp
  90% of sequence in fragments shorter than    6896 bp
  95% of sequence in fragments shorter than    8678 bp

# EsaBC3I.SmaI (TCGA+CCCGGG) double digest, 1979 thousand fragments (1603346+376940 sites), 1505 bp average fragment length
   5% of sequence in fragments shorter than     499 bp
  10% of sequence in fragments shorter than     791 bp
  15% of sequence in fragments shorter than    1050 bp
  20% of sequence in fragments shorter than    1299 bp
  25% of sequence in fragments shorter than    1540 bp
  30% of sequence in fragments shorter than    1794 bp
  35% of sequence in fragments shorter than    2060 bp
  40% of sequence in fragments shorter than    2336 bp
  45% of sequence in fragments shorter than    2629 bp
  50% of sequence in fragments shorter than    2940 bp
  55% of sequence in fragments shorter than    3276 bp
  60% of sequence in fragments shorter than    3651 bp
  65% of sequence in fragments shorter than    4067 bp
  70% of sequence in fragments shorter than    4547 bp
  75% of sequence in fragments shorter than    5105 bp
  80% of sequence in fragments shorter than    5792 bp
  85% of sequence in fragments shorter than    6683 bp
  90% of sequence in fragments shorter than    7927 bp
  95% of sequence in fragments shorter than   10051 bp

# BclI.BscGI (TGATCA+CCCGT) double digest, 1969 thousand fragments (738785+1231070 sites), 1513 bp average fragment length
   5% of sequence in fragments shorter than     466 bp
  10% of sequence in fragments shorter than     762 bp
  15% of sequence in fragments shorter than    1029 bp
  20% of sequence in fragments shorter than    1281 bp
  25% of sequence in fragments shorter than    1527 bp
  30% of sequence in fragments shorter than    1772 bp
  35% of sequence in fragments shorter than    2028 bp
  40% of sequence in fragments shorter than    2290 bp
  45% of sequence in fragments shorter than    2561 bp
  50% of sequence in fragments shorter than    2846 bp
  55% of sequence in fragments shorter than    3162 bp
  60% of sequence in fragments shorter than    3504 bp
  65% of sequence in fragments shorter than    3883 bp
  70% of sequence in fragments shorter than    4313 bp
  75% of sequence in fragments shorter than    4816 bp
  80% of sequence in fragments shorter than    5418 bp
  85% of sequence in fragments shorter than    6186 bp
  90% of sequence in fragments shorter than    7255 bp
  95% of sequence in fragments shorter than    9071 bp

# BamHI.EsaBC3I (GGATCC+TCGA) double digest, 1968 thousand fragments (365999+1603346 sites), 1513 bp average fragment length
   5% of sequence in fragments shorter than     519 bp
  10% of sequence in fragments shorter than     795 bp
  15% of sequence in fragments shorter than    1036 bp
  20% of sequence in fragments shorter than    1262 bp
  25% of sequence in fragments shorter than    1477 bp
  30% of sequence in fragments shorter than    1700 bp
  35% of sequence in fragments shorter than    1929 bp
  40% of sequence in fragments shorter than    2173 bp
  45% of sequence in fragments shorter than    2428 bp
  50% of sequence in fragments shorter than    2700 bp
  55% of sequence in fragments shorter than    2985 bp
  60% of sequence in fragments shorter than    3307 bp
  65% of sequence in fragments shorter than    3662 bp
  70% of sequence in fragments shorter than    4069 bp
  75% of sequence in fragments shorter than    4545 bp
  80% of sequence in fragments shorter than    5124 bp
  85% of sequence in fragments shorter than    5872 bp
  90% of sequence in fragments shorter than    6924 bp
  95% of sequence in fragments shorter than    8732 bp

# BscGI.FnuDII (CCCGT+CGCG) double digest, 1964 thousand fragments (1231070+733940 sites), 1516 bp average fragment length
   5% of sequence in fragments shorter than     450 bp
  10% of sequence in fragments shorter than     856 bp
  15% of sequence in fragments shorter than    1245 bp
  20% of sequence in fragments shorter than    1639 bp
  25% of sequence in fragments shorter than    2045 bp
  30% of sequence in fragments shorter than    2470 bp
  35% of sequence in fragments shorter than    2914 bp
  40% of sequence in fragments shorter than    3391 bp
  45% of sequence in fragments shorter than    3902 bp
  50% of sequence in fragments shorter than    4457 bp
  55% of sequence in fragments shorter than    5077 bp
  60% of sequence in fragments shorter than    5758 bp
  65% of sequence in fragments shorter than    6527 bp
  70% of sequence in fragments shorter than    7413 bp
  75% of sequence in fragments shorter than    8457 bp
  80% of sequence in fragments shorter than    9749 bp
  85% of sequence in fragments shorter than   11409 bp
  90% of sequence in fragments shorter than   13739 bp
  95% of sequence in fragments shorter than   17862 bp

# AflII.PstI (CTTAAG+CTGCAG) double digest, 1964 thousand fragments (643284+1321469 sites), 1517 bp average fragment length
   5% of sequence in fragments shorter than     524 bp
  10% of sequence in fragments shorter than     796 bp
  15% of sequence in fragments shorter than    1040 bp
  20% of sequence in fragments shorter than    1272 bp
  25% of sequence in fragments shorter than    1497 bp
  30% of sequence in fragments shorter than    1727 bp
  35% of sequence in fragments shorter than    1959 bp
  40% of sequence in fragments shorter than    2195 bp
  45% of sequence in fragments shorter than    2445 bp
  50% of sequence in fragments shorter than    2710 bp
  55% of sequence in fragments shorter than    2971 bp
  60% of sequence in fragments shorter than    3283 bp
  65% of sequence in fragments shorter than    3618 bp
  70% of sequence in fragments shorter than    4008 bp
  75% of sequence in fragments shorter than    4470 bp
  80% of sequence in fragments shorter than    5031 bp
  85% of sequence in fragments shorter than    5745 bp
  90% of sequence in fragments shorter than    6772 bp
  95% of sequence in fragments shorter than    8672 bp

# AccI.HindIII (GTMKAC+AAGCTT) double digest, 1960 thousand fragments (1101063+860365 sites), 1519 bp average fragment length
   5% of sequence in fragments shorter than     532 bp
  10% of sequence in fragments shorter than     805 bp
  15% of sequence in fragments shorter than    1043 bp
  20% of sequence in fragments shorter than    1261 bp
  25% of sequence in fragments shorter than    1471 bp
  30% of sequence in fragments shorter than    1691 bp
  35% of sequence in fragments shorter than    1902 bp
  40% of sequence in fragments shorter than    2114 bp
  45% of sequence in fragments shorter than    2337 bp
  50% of sequence in fragments shorter than    2583 bp
  55% of sequence in fragments shorter than    2852 bp
  60% of sequence in fragments shorter than    3148 bp
  65% of sequence in fragments shorter than    3482 bp
  70% of sequence in fragments shorter than    3858 bp
  75% of sequence in fragments shorter than    4293 bp
  80% of sequence in fragments shorter than    4826 bp
  85% of sequence in fragments shorter than    5523 bp
  90% of sequence in fragments shorter than    6527 bp
  95% of sequence in fragments shorter than    8397 bp

# AccI.XbaI (GTMKAC+TCTAGA) double digest, 1951 thousand fragments (1101063+850998 sites), 1526 bp average fragment length
   5% of sequence in fragments shorter than     515 bp
  10% of sequence in fragments shorter than     798 bp
  15% of sequence in fragments shorter than    1030 bp
  20% of sequence in fragments shorter than    1264 bp
  25% of sequence in fragments shorter than    1476 bp
  30% of sequence in fragments shorter than    1702 bp
  35% of sequence in fragments shorter than    1924 bp
  40% of sequence in fragments shorter than    2163 bp
  45% of sequence in fragments shorter than    2402 bp
  50% of sequence in fragments shorter than    2666 bp
  55% of sequence in fragments shorter than    2941 bp
  60% of sequence in fragments shorter than    3251 bp
  65% of sequence in fragments shorter than    3575 bp
  70% of sequence in fragments shorter than    3958 bp
  75% of sequence in fragments shorter than    4412 bp
  80% of sequence in fragments shorter than    4963 bp
  85% of sequence in fragments shorter than    5673 bp
  90% of sequence in fragments shorter than    6693 bp
  95% of sequence in fragments shorter than    8504 bp

# HaeII.PstI (RGCGCY+CTGCAG) double digest, 1951 thousand fragments (630101+1321469 sites), 1527 bp average fragment length
   5% of sequence in fragments shorter than     486 bp
  10% of sequence in fragments shorter than     765 bp
  15% of sequence in fragments shorter than    1041 bp
  20% of sequence in fragments shorter than    1327 bp
  25% of sequence in fragments shorter than    1603 bp
  30% of sequence in fragments shorter than    1896 bp
  35% of sequence in fragments shorter than    2200 bp
  40% of sequence in fragments shorter than    2521 bp
  45% of sequence in fragments shorter than    2870 bp
  50% of sequence in fragments shorter than    3247 bp
  55% of sequence in fragments shorter than    3655 bp
  60% of sequence in fragments shorter than    4109 bp
  65% of sequence in fragments shorter than    4617 bp
  70% of sequence in fragments shorter than    5187 bp
  75% of sequence in fragments shorter than    5850 bp
  80% of sequence in fragments shorter than    6663 bp
  85% of sequence in fragments shorter than    7697 bp
  90% of sequence in fragments shorter than    9154 bp
  95% of sequence in fragments shorter than   11719 bp

# HgiCI.SpeI (GGYRCC+ACTAGT) double digest, 1950 thousand fragments (1550877+400286 sites), 1527 bp average fragment length
   5% of sequence in fragments shorter than     519 bp
  10% of sequence in fragments shorter than     787 bp
  15% of sequence in fragments shorter than    1036 bp
  20% of sequence in fragments shorter than    1251 bp
  25% of sequence in fragments shorter than    1478 bp
  30% of sequence in fragments shorter than    1703 bp
  35% of sequence in fragments shorter than    1943 bp
  40% of sequence in fragments shorter than    2187 bp
  45% of sequence in fragments shorter than    2452 bp
  50% of sequence in fragments shorter than    2730 bp
  55% of sequence in fragments shorter than    3020 bp
  60% of sequence in fragments shorter than    3346 bp
  65% of sequence in fragments shorter than    3717 bp
  70% of sequence in fragments shorter than    4139 bp
  75% of sequence in fragments shorter than    4635 bp
  80% of sequence in fragments shorter than    5248 bp
  85% of sequence in fragments shorter than    6042 bp
  90% of sequence in fragments shorter than    7206 bp
  95% of sequence in fragments shorter than    9477 bp

# HaeII.HgiCI (RGCGCY+GGYRCC) double digest, 1950 thousand fragments (630101+1550877 sites), 1528 bp average fragment length
   5% of sequence in fragments shorter than     487 bp
  10% of sequence in fragments shorter than     772 bp
  15% of sequence in fragments shorter than    1043 bp
  20% of sequence in fragments shorter than    1299 bp
  25% of sequence in fragments shorter than    1570 bp
  30% of sequence in fragments shorter than    1842 bp
  35% of sequence in fragments shorter than    2125 bp
  40% of sequence in fragments shorter than    2434 bp
  45% of sequence in fragments shorter than    2759 bp
  50% of sequence in fragments shorter than    3098 bp
  55% of sequence in fragments shorter than    3477 bp
  60% of sequence in fragments shorter than    3901 bp
  65% of sequence in fragments shorter than    4372 bp
  70% of sequence in fragments shorter than    4918 bp
  75% of sequence in fragments shorter than    5559 bp
  80% of sequence in fragments shorter than    6335 bp
  85% of sequence in fragments shorter than    7364 bp
  90% of sequence in fragments shorter than    8780 bp
  95% of sequence in fragments shorter than   11270 bp

# BsmI.SpeI (GAATGC+ACTAGT) double digest, 1949 thousand fragments (1549350+400286 sites), 1528 bp average fragment length
   5% of sequence in fragments shorter than     519 bp
  10% of sequence in fragments shorter than     885 bp
  15% of sequence in fragments shorter than    1174 bp
  20% of sequence in fragments shorter than    1429 bp
  25% of sequence in fragments shorter than    1691 bp
  30% of sequence in fragments shorter than    1952 bp
  35% of sequence in fragments shorter than    2218 bp
  40% of sequence in fragments shorter than    2484 bp
  45% of sequence in fragments shorter than    2760 bp
  50% of sequence in fragments shorter than    3055 bp
  55% of sequence in fragments shorter than    3374 bp
  60% of sequence in fragments shorter than    3723 bp
  65% of sequence in fragments shorter than    4106 bp
  70% of sequence in fragments shorter than    4532 bp
  75% of sequence in fragments shorter than    5038 bp
  80% of sequence in fragments shorter than    5634 bp
  85% of sequence in fragments shorter than    6398 bp
  90% of sequence in fragments shorter than    7445 bp
  95% of sequence in fragments shorter than    9271 bp

# AccI.EcoRI (GTMKAC+GAATTC) double digest, 1947 thousand fragments (1101063+847401 sites), 1529 bp average fragment length
   5% of sequence in fragments shorter than     524 bp
  10% of sequence in fragments shorter than     801 bp
  15% of sequence in fragments shorter than    1048 bp
  20% of sequence in fragments shorter than    1278 bp
  25% of sequence in fragments shorter than    1496 bp
  30% of sequence in fragments shorter than    1722 bp
  35% of sequence in fragments shorter than    1946 bp
  40% of sequence in fragments shorter than    2181 bp
  45% of sequence in fragments shorter than    2423 bp
  50% of sequence in fragments shorter than    2660 bp
  55% of sequence in fragments shorter than    2932 bp
  60% of sequence in fragments shorter than    3234 bp
  65% of sequence in fragments shorter than    3555 bp
  70% of sequence in fragments shorter than    3933 bp
  75% of sequence in fragments shorter than    4377 bp
  80% of sequence in fragments shorter than    4915 bp
  85% of sequence in fragments shorter than    5605 bp
  90% of sequence in fragments shorter than    6579 bp
  95% of sequence in fragments shorter than    8320 bp

# PsiI.SexAI (TTATAA+ACCWGGT) double digest, 1945 thousand fragments (1647912+297900 sites), 1531 bp average fragment length
   5% of sequence in fragments shorter than     521 bp
  10% of sequence in fragments shorter than     806 bp
  15% of sequence in fragments shorter than    1059 bp
  20% of sequence in fragments shorter than    1298 bp
  25% of sequence in fragments shorter than    1533 bp
  30% of sequence in fragments shorter than    1774 bp
  35% of sequence in fragments shorter than    2020 bp
  40% of sequence in fragments shorter than    2281 bp
  45% of sequence in fragments shorter than    2559 bp
  50% of sequence in fragments shorter than    2852 bp
  55% of sequence in fragments shorter than    3158 bp
  60% of sequence in fragments shorter than    3513 bp
  65% of sequence in fragments shorter than    3916 bp
  70% of sequence in fragments shorter than    4381 bp
  75% of sequence in fragments shorter than    4948 bp
  80% of sequence in fragments shorter than    5657 bp
  85% of sequence in fragments shorter than    6610 bp
  90% of sequence in fragments shorter than    8064 bp
  95% of sequence in fragments shorter than   11231 bp

# AflIII.ApaI (ACRYGT+GGGCCC) double digest, 1945 thousand fragments (1485394+460339 sites), 1531 bp average fragment length
   5% of sequence in fragments shorter than     532 bp
  10% of sequence in fragments shorter than     837 bp
  15% of sequence in fragments shorter than    1100 bp
  20% of sequence in fragments shorter than    1348 bp
  25% of sequence in fragments shorter than    1593 bp
  30% of sequence in fragments shorter than    1838 bp
  35% of sequence in fragments shorter than    2084 bp
  40% of sequence in fragments shorter than    2335 bp
  45% of sequence in fragments shorter than    2603 bp
  50% of sequence in fragments shorter than    2883 bp
  55% of sequence in fragments shorter than    3183 bp
  60% of sequence in fragments shorter than    3519 bp
  65% of sequence in fragments shorter than    3895 bp
  70% of sequence in fragments shorter than    4319 bp
  75% of sequence in fragments shorter than    4816 bp
  80% of sequence in fragments shorter than    5416 bp
  85% of sequence in fragments shorter than    6160 bp
  90% of sequence in fragments shorter than    7215 bp
  95% of sequence in fragments shorter than    9175 bp

# HindIII.PpuMI (AAGCTT+RGGWCCY) double digest, 1945 thousand fragments (860365+1085138 sites), 1532 bp average fragment length
   5% of sequence in fragments shorter than     522 bp
  10% of sequence in fragments shorter than     797 bp
  15% of sequence in fragments shorter than    1050 bp
  20% of sequence in fragments shorter than    1291 bp
  25% of sequence in fragments shorter than    1531 bp
  30% of sequence in fragments shorter than    1772 bp
  35% of sequence in fragments shorter than    1994 bp
  40% of sequence in fragments shorter than    2248 bp
  45% of sequence in fragments shorter than    2514 bp
  50% of sequence in fragments shorter than    2792 bp
  55% of sequence in fragments shorter than    3092 bp
  60% of sequence in fragments shorter than    3424 bp
  65% of sequence in fragments shorter than    3796 bp
  70% of sequence in fragments shorter than    4219 bp
  75% of sequence in fragments shorter than    4713 bp
  80% of sequence in fragments shorter than    5313 bp
  85% of sequence in fragments shorter than    6092 bp
  90% of sequence in fragments shorter than    7217 bp
  95% of sequence in fragments shorter than    9360 bp

# BsaAI.BscGI (YACGTR+CCCGT) double digest, 1944 thousand fragments (713801+1231070 sites), 1532 bp average fragment length
   5% of sequence in fragments shorter than     463 bp
  10% of sequence in fragments shorter than     770 bp
  15% of sequence in fragments shorter than    1051 bp
  20% of sequence in fragments shorter than    1331 bp
  25% of sequence in fragments shorter than    1607 bp
  30% of sequence in fragments shorter than    1887 bp
  35% of sequence in fragments shorter than    2177 bp
  40% of sequence in fragments shorter than    2480 bp
  45% of sequence in fragments shorter than    2801 bp
  50% of sequence in fragments shorter than    3146 bp
  55% of sequence in fragments shorter than    3500 bp
  60% of sequence in fragments shorter than    3902 bp
  65% of sequence in fragments shorter than    4347 bp
  70% of sequence in fragments shorter than    4853 bp
  75% of sequence in fragments shorter than    5453 bp
  80% of sequence in fragments shorter than    6184 bp
  85% of sequence in fragments shorter than    7122 bp
  90% of sequence in fragments shorter than    8432 bp
  95% of sequence in fragments shorter than   10687 bp

# HindIII.PvuII (AAGCTT+CAGCTG) double digest, 1944 thousand fragments (860365+1084260 sites), 1532 bp average fragment length
   5% of sequence in fragments shorter than     524 bp
  10% of sequence in fragments shorter than     810 bp
  15% of sequence in fragments shorter than    1051 bp
  20% of sequence in fragments shorter than    1286 bp
  25% of sequence in fragments shorter than    1516 bp
  30% of sequence in fragments shorter than    1742 bp
  35% of sequence in fragments shorter than    1960 bp
  40% of sequence in fragments shorter than    2204 bp
  45% of sequence in fragments shorter than    2459 bp
  50% of sequence in fragments shorter than    2728 bp
  55% of sequence in fragments shorter than    3015 bp
  60% of sequence in fragments shorter than    3331 bp
  65% of sequence in fragments shorter than    3680 bp
  70% of sequence in fragments shorter than    4078 bp
  75% of sequence in fragments shorter than    4543 bp
  80% of sequence in fragments shorter than    5100 bp
  85% of sequence in fragments shorter than    5826 bp
  90% of sequence in fragments shorter than    6874 bp
  95% of sequence in fragments shorter than    8819 bp

# BfiI.BsrBI (ACTGGG+CCGCTC) double digest, 1941 thousand fragments (1706594+235523 sites), 1534 bp average fragment length
   5% of sequence in fragments shorter than     510 bp
  10% of sequence in fragments shorter than     802 bp
  15% of sequence in fragments shorter than    1053 bp
  20% of sequence in fragments shorter than    1301 bp
  25% of sequence in fragments shorter than    1545 bp
  30% of sequence in fragments shorter than    1787 bp
  35% of sequence in fragments shorter than    2033 bp
  40% of sequence in fragments shorter than    2279 bp
  45% of sequence in fragments shorter than    2556 bp
  50% of sequence in fragments shorter than    2825 bp
  55% of sequence in fragments shorter than    3143 bp
  60% of sequence in fragments shorter than    3493 bp
  65% of sequence in fragments shorter than    3887 bp
  70% of sequence in fragments shorter than    4334 bp
  75% of sequence in fragments shorter than    4854 bp
  80% of sequence in fragments shorter than    5481 bp
  85% of sequence in fragments shorter than    6305 bp
  90% of sequence in fragments shorter than    7496 bp
  95% of sequence in fragments shorter than    9759 bp

# HgiCI.HpaI (GGYRCC+GTTAAC) double digest, 1939 thousand fragments (1550877+389418 sites), 1536 bp average fragment length
   5% of sequence in fragments shorter than     517 bp
  10% of sequence in fragments shorter than     795 bp
  15% of sequence in fragments shorter than    1042 bp
  20% of sequence in fragments shorter than    1260 bp
  25% of sequence in fragments shorter than    1492 bp
  30% of sequence in fragments shorter than    1723 bp
  35% of sequence in fragments shorter than    1963 bp
  40% of sequence in fragments shorter than    2216 bp
  45% of sequence in fragments shorter than    2484 bp
  50% of sequence in fragments shorter than    2767 bp
  55% of sequence in fragments shorter than    3070 bp
  60% of sequence in fragments shorter than    3406 bp
  65% of sequence in fragments shorter than    3787 bp
  70% of sequence in fragments shorter than    4222 bp
  75% of sequence in fragments shorter than    4736 bp
  80% of sequence in fragments shorter than    5375 bp
  85% of sequence in fragments shorter than    6208 bp
  90% of sequence in fragments shorter than    7451 bp
  95% of sequence in fragments shorter than   10003 bp

# BstEII.EsaBC3I (GGTNACC+TCGA) double digest, 1938 thousand fragments (335865+1603346 sites), 1537 bp average fragment length
   5% of sequence in fragments shorter than     521 bp
  10% of sequence in fragments shorter than     803 bp
  15% of sequence in fragments shorter than    1049 bp
  20% of sequence in fragments shorter than    1281 bp
  25% of sequence in fragments shorter than    1503 bp
  30% of sequence in fragments shorter than    1736 bp
  35% of sequence in fragments shorter than    1976 bp
  40% of sequence in fragments shorter than    2230 bp
  45% of sequence in fragments shorter than    2496 bp
  50% of sequence in fragments shorter than    2775 bp
  55% of sequence in fragments shorter than    3077 bp
  60% of sequence in fragments shorter than    3403 bp
  65% of sequence in fragments shorter than    3771 bp
  70% of sequence in fragments shorter than    4191 bp
  75% of sequence in fragments shorter than    4685 bp
  80% of sequence in fragments shorter than    5279 bp
  85% of sequence in fragments shorter than    6040 bp
  90% of sequence in fragments shorter than    7107 bp
  95% of sequence in fragments shorter than    8958 bp

# AvaI.AvaIII (CYCGRG+ATGCAT) double digest, 1938 thousand fragments (1005714+933357 sites), 1537 bp average fragment length
   5% of sequence in fragments shorter than     530 bp
  10% of sequence in fragments shorter than     822 bp
  15% of sequence in fragments shorter than    1078 bp
  20% of sequence in fragments shorter than    1321 bp
  25% of sequence in fragments shorter than    1561 bp
  30% of sequence in fragments shorter than    1802 bp
  35% of sequence in fragments shorter than    2050 bp
  40% of sequence in fragments shorter than    2300 bp
  45% of sequence in fragments shorter than    2573 bp
  50% of sequence in fragments shorter than    2860 bp
  55% of sequence in fragments shorter than    3176 bp
  60% of sequence in fragments shorter than    3522 bp
  65% of sequence in fragments shorter than    3913 bp
  70% of sequence in fragments shorter than    4352 bp
  75% of sequence in fragments shorter than    4863 bp
  80% of sequence in fragments shorter than    5477 bp
  85% of sequence in fragments shorter than    6282 bp
  90% of sequence in fragments shorter than    7448 bp
  95% of sequence in fragments shorter than    9697 bp

# BsmI.HpaI (GAATGC+GTTAAC) double digest, 1938 thousand fragments (1549350+389418 sites), 1537 bp average fragment length
   5% of sequence in fragments shorter than     519 bp
  10% of sequence in fragments shorter than     888 bp
  15% of sequence in fragments shorter than    1179 bp
  20% of sequence in fragments shorter than    1454 bp
  25% of sequence in fragments shorter than    1712 bp
  30% of sequence in fragments shorter than    1974 bp
  35% of sequence in fragments shorter than    2237 bp
  40% of sequence in fragments shorter than    2507 bp
  45% of sequence in fragments shorter than    2785 bp
  50% of sequence in fragments shorter than    3080 bp
  55% of sequence in fragments shorter than    3398 bp
  60% of sequence in fragments shorter than    3744 bp
  65% of sequence in fragments shorter than    4126 bp
  70% of sequence in fragments shorter than    4555 bp
  75% of sequence in fragments shorter than    5053 bp
  80% of sequence in fragments shorter than    5649 bp
  85% of sequence in fragments shorter than    6400 bp
  90% of sequence in fragments shorter than    7439 bp
  95% of sequence in fragments shorter than    9225 bp

# BfiI.KasI (ACTGGG+GGCGCC) double digest, 1936 thousand fragments (1706594+230381 sites), 1538 bp average fragment length
   5% of sequence in fragments shorter than     515 bp
  10% of sequence in fragments shorter than     804 bp
  15% of sequence in fragments shorter than    1051 bp
  20% of sequence in fragments shorter than    1295 bp
  25% of sequence in fragments shorter than    1534 bp
  30% of sequence in fragments shorter than    1771 bp
  35% of sequence in fragments shorter than    2019 bp
  40% of sequence in fragments shorter than    2255 bp
  45% of sequence in fragments shorter than    2526 bp
  50% of sequence in fragments shorter than    2789 bp
  55% of sequence in fragments shorter than    3100 bp
  60% of sequence in fragments shorter than    3444 bp
  65% of sequence in fragments shorter than    3829 bp
  70% of sequence in fragments shorter than    4268 bp
  75% of sequence in fragments shorter than    4781 bp
  80% of sequence in fragments shorter than    5390 bp
  85% of sequence in fragments shorter than    6203 bp
  90% of sequence in fragments shorter than    7417 bp
  95% of sequence in fragments shorter than    9774 bp

# BceAI.BscGI (ACGGC+CCCGT) double digest, 1935 thousand fragments (705178+1231070 sites), 1539 bp average fragment length
   5% of sequence in fragments shorter than     433 bp
  10% of sequence in fragments shorter than     756 bp
  15% of sequence in fragments shorter than    1071 bp
  20% of sequence in fragments shorter than    1385 bp
  25% of sequence in fragments shorter than    1706 bp
  30% of sequence in fragments shorter than    2036 bp
  35% of sequence in fragments shorter than    2381 bp
  40% of sequence in fragments shorter than    2748 bp
  45% of sequence in fragments shorter than    3142 bp
  50% of sequence in fragments shorter than    3570 bp
  55% of sequence in fragments shorter than    4035 bp
  60% of sequence in fragments shorter than    4550 bp
  65% of sequence in fragments shorter than    5128 bp
  70% of sequence in fragments shorter than    5793 bp
  75% of sequence in fragments shorter than    6573 bp
  80% of sequence in fragments shorter than    7513 bp
  85% of sequence in fragments shorter than    8758 bp
  90% of sequence in fragments shorter than   10500 bp
  95% of sequence in fragments shorter than   13484 bp

# PpuMI.XbaI (RGGWCCY+TCTAGA) double digest, 1935 thousand fragments (1085138+850998 sites), 1539 bp average fragment length
   5% of sequence in fragments shorter than     529 bp
  10% of sequence in fragments shorter than     820 bp
  15% of sequence in fragments shorter than    1058 bp
  20% of sequence in fragments shorter than    1280 bp
  25% of sequence in fragments shorter than    1517 bp
  30% of sequence in fragments shorter than    1753 bp
  35% of sequence in fragments shorter than    1996 bp
  40% of sequence in fragments shorter than    2246 bp
  45% of sequence in fragments shorter than    2499 bp
  50% of sequence in fragments shorter than    2775 bp
  55% of sequence in fragments shorter than    3075 bp
  60% of sequence in fragments shorter than    3405 bp
  65% of sequence in fragments shorter than    3771 bp
  70% of sequence in fragments shorter than    4192 bp
  75% of sequence in fragments shorter than    4682 bp
  80% of sequence in fragments shorter than    5270 bp
  85% of sequence in fragments shorter than    6033 bp
  90% of sequence in fragments shorter than    7098 bp
  95% of sequence in fragments shorter than    8993 bp

# ApaLI.FauI (GTGCAC+CCCGC) double digest, 1935 thousand fragments (496255+1439772 sites), 1539 bp average fragment length
   5% of sequence in fragments shorter than     524 bp
  10% of sequence in fragments shorter than     895 bp
  15% of sequence in fragments shorter than    1241 bp
  20% of sequence in fragments shorter than    1558 bp
  25% of sequence in fragments shorter than    1905 bp
  30% of sequence in fragments shorter than    2257 bp
  35% of sequence in fragments shorter than    2634 bp
  40% of sequence in fragments shorter than    3034 bp
  45% of sequence in fragments shorter than    3462 bp
  50% of sequence in fragments shorter than    3930 bp
  55% of sequence in fragments shorter than    4416 bp
  60% of sequence in fragments shorter than    4988 bp
  65% of sequence in fragments shorter than    5619 bp
  70% of sequence in fragments shorter than    6332 bp
  75% of sequence in fragments shorter than    7199 bp
  80% of sequence in fragments shorter than    8265 bp
  85% of sequence in fragments shorter than    9640 bp
  90% of sequence in fragments shorter than   11631 bp
  95% of sequence in fragments shorter than   15311 bp

# KpnI.PsiI (GGTACC+TTATAA) double digest, 1935 thousand fragments (287974+1647912 sites), 1539 bp average fragment length
   5% of sequence in fragments shorter than     529 bp
  10% of sequence in fragments shorter than     809 bp
  15% of sequence in fragments shorter than    1049 bp
  20% of sequence in fragments shorter than    1276 bp
  25% of sequence in fragments shorter than    1514 bp
  30% of sequence in fragments shorter than    1751 bp
  35% of sequence in fragments shorter than    1991 bp
  40% of sequence in fragments shorter than    2244 bp
  45% of sequence in fragments shorter than    2522 bp
  50% of sequence in fragments shorter than    2816 bp
  55% of sequence in fragments shorter than    3139 bp
  60% of sequence in fragments shorter than    3509 bp
  65% of sequence in fragments shorter than    3924 bp
  70% of sequence in fragments shorter than    4416 bp
  75% of sequence in fragments shorter than    5018 bp
  80% of sequence in fragments shorter than    5783 bp
  85% of sequence in fragments shorter than    6859 bp
  90% of sequence in fragments shorter than    8570 bp
  95% of sequence in fragments shorter than   12421 bp

# NheI.PsiI (GCTAGC+TTATAA) double digest, 1934 thousand fragments (287384+1647912 sites), 1540 bp average fragment length
   5% of sequence in fragments shorter than     524 bp
  10% of sequence in fragments shorter than     801 bp
  15% of sequence in fragments shorter than    1054 bp
  20% of sequence in fragments shorter than    1298 bp
  25% of sequence in fragments shorter than    1535 bp
  30% of sequence in fragments shorter than    1763 bp
  35% of sequence in fragments shorter than    2002 bp
  40% of sequence in fragments shorter than    2260 bp
  45% of sequence in fragments shorter than    2524 bp
  50% of sequence in fragments shorter than    2821 bp
  55% of sequence in fragments shorter than    3147 bp
  60% of sequence in fragments shorter than    3520 bp
  65% of sequence in fragments shorter than    3950 bp
  70% of sequence in fragments shorter than    4457 bp
  75% of sequence in fragments shorter than    5082 bp
  80% of sequence in fragments shorter than    5879 bp
  85% of sequence in fragments shorter than    7010 bp
  90% of sequence in fragments shorter than    8832 bp
  95% of sequence in fragments shorter than   13130 bp

# PvuII.XbaI (CAGCTG+TCTAGA) double digest, 1934 thousand fragments (1084260+850998 sites), 1540 bp average fragment length
   5% of sequence in fragments shorter than     538 bp
  10% of sequence in fragments shorter than     824 bp
  15% of sequence in fragments shorter than    1053 bp
  20% of sequence in fragments shorter than    1290 bp
  25% of sequence in fragments shorter than    1501 bp
  30% of sequence in fragments shorter than    1723 bp
  35% of sequence in fragments shorter than    1952 bp
  40% of sequence in fragments shorter than    2191 bp
  45% of sequence in fragments shorter than    2432 bp
  50% of sequence in fragments shorter than    2681 bp
  55% of sequence in fragments shorter than    2958 bp
  60% of sequence in fragments shorter than    3270 bp
  65% of sequence in fragments shorter than    3608 bp
  70% of sequence in fragments shorter than    3997 bp
  75% of sequence in fragments shorter than    4450 bp
  80% of sequence in fragments shorter than    4991 bp
  85% of sequence in fragments shorter than    5689 bp
  90% of sequence in fragments shorter than    6662 bp
  95% of sequence in fragments shorter than    8401 bp

# ApaLI.BspMI (GTGCAC+ACCTGC) double digest, 1933 thousand fragments (496255+1438129 sites), 1540 bp average fragment length
   5% of sequence in fragments shorter than     525 bp
  10% of sequence in fragments shorter than     823 bp
  15% of sequence in fragments shorter than    1077 bp
  20% of sequence in fragments shorter than    1329 bp
  25% of sequence in fragments shorter than    1569 bp
  30% of sequence in fragments shorter than    1821 bp
  35% of sequence in fragments shorter than    2059 bp
  40% of sequence in fragments shorter than    2324 bp
  45% of sequence in fragments shorter than    2608 bp
  50% of sequence in fragments shorter than    2901 bp
  55% of sequence in fragments shorter than    3226 bp
  60% of sequence in fragments shorter than    3531 bp
  65% of sequence in fragments shorter than    3926 bp
  70% of sequence in fragments shorter than    4375 bp
  75% of sequence in fragments shorter than    4901 bp
  80% of sequence in fragments shorter than    5526 bp
  85% of sequence in fragments shorter than    6331 bp
  90% of sequence in fragments shorter than    7485 bp
  95% of sequence in fragments shorter than    9501 bp

# BfiI.SwaI (ACTGGG+ATTTAAAT) double digest, 1932 thousand fragments (1706594+226602 sites), 1541 bp average fragment length
   5% of sequence in fragments shorter than     529 bp
  10% of sequence in fragments shorter than     811 bp
  15% of sequence in fragments shorter than    1051 bp
  20% of sequence in fragments shorter than    1286 bp
  25% of sequence in fragments shorter than    1513 bp
  30% of sequence in fragments shorter than    1738 bp
  35% of sequence in fragments shorter than    1968 bp
  40% of sequence in fragments shorter than    2193 bp
  45% of sequence in fragments shorter than    2438 bp
  50% of sequence in fragments shorter than    2706 bp
  55% of sequence in fragments shorter than    2964 bp
  60% of sequence in fragments shorter than    3274 bp
  65% of sequence in fragments shorter than    3617 bp
  70% of sequence in fragments shorter than    4011 bp
  75% of sequence in fragments shorter than    4468 bp
  80% of sequence in fragments shorter than    5008 bp
  85% of sequence in fragments shorter than    5713 bp
  90% of sequence in fragments shorter than    6750 bp
  95% of sequence in fragments shorter than    8756 bp

# EcoRI.PpuMI (GAATTC+RGGWCCY) double digest, 1932 thousand fragments (847401+1085138 sites), 1542 bp average fragment length
   5% of sequence in fragments shorter than     515 bp
  10% of sequence in fragments shorter than     801 bp
  15% of sequence in fragments shorter than    1051 bp
  20% of sequence in fragments shorter than    1304 bp
  25% of sequence in fragments shorter than    1546 bp
  30% of sequence in fragments shorter than    1794 bp
  35% of sequence in fragments shorter than    2043 bp
  40% of sequence in fragments shorter than    2302 bp
  45% of sequence in fragments shorter than    2575 bp
  50% of sequence in fragments shorter than    2847 bp
  55% of sequence in fragments shorter than    3155 bp
  60% of sequence in fragments shorter than    3489 bp
  65% of sequence in fragments shorter than    3858 bp
  70% of sequence in fragments shorter than    4287 bp
  75% of sequence in fragments shorter than    4784 bp
  80% of sequence in fragments shorter than    5390 bp
  85% of sequence in fragments shorter than    6162 bp
  90% of sequence in fragments shorter than    7257 bp
  95% of sequence in fragments shorter than    9221 bp

# AflIII.EcoRV (ACRYGT+GATATC) double digest, 1931 thousand fragments (1485394+446473 sites), 1542 bp average fragment length
   5% of sequence in fragments shorter than     548 bp
  10% of sequence in fragments shorter than     847 bp
  15% of sequence in fragments shorter than    1106 bp
  20% of sequence in fragments shorter than    1350 bp
  25% of sequence in fragments shorter than    1588 bp
  30% of sequence in fragments shorter than    1821 bp
  35% of sequence in fragments shorter than    2056 bp
  40% of sequence in fragments shorter than    2293 bp
  45% of sequence in fragments shorter than    2534 bp
  50% of sequence in fragments shorter than    2800 bp
  55% of sequence in fragments shorter than    3083 bp
  60% of sequence in fragments shorter than    3397 bp
  65% of sequence in fragments shorter than    3748 bp
  70% of sequence in fragments shorter than    4152 bp
  75% of sequence in fragments shorter than    4615 bp
  80% of sequence in fragments shorter than    5177 bp
  85% of sequence in fragments shorter than    5901 bp
  90% of sequence in fragments shorter than    6934 bp
  95% of sequence in fragments shorter than    8837 bp

# EcoRI.PvuII (GAATTC+CAGCTG) double digest, 1931 thousand fragments (847401+1084260 sites), 1543 bp average fragment length
   5% of sequence in fragments shorter than     521 bp
  10% of sequence in fragments shorter than     805 bp
  15% of sequence in fragments shorter than    1058 bp
  20% of sequence in fragments shorter than    1301 bp
  25% of sequence in fragments shorter than    1535 bp
  30% of sequence in fragments shorter than    1772 bp
  35% of sequence in fragments shorter than    2011 bp
  40% of sequence in fragments shorter than    2264 bp
  45% of sequence in fragments shorter than    2528 bp
  50% of sequence in fragments shorter than    2786 bp
  55% of sequence in fragments shorter than    3080 bp
  60% of sequence in fragments shorter than    3403 bp
  65% of sequence in fragments shorter than    3757 bp
  70% of sequence in fragments shorter than    4161 bp
  75% of sequence in fragments shorter than    4628 bp
  80% of sequence in fragments shorter than    5198 bp
  85% of sequence in fragments shorter than    5932 bp
  90% of sequence in fragments shorter than    6955 bp
  95% of sequence in fragments shorter than    8782 bp

# AvaI.StuI (CYCGRG+AGGCCT) double digest, 1930 thousand fragments (1005714+925729 sites), 1543 bp average fragment length
   5% of sequence in fragments shorter than     483 bp
  10% of sequence in fragments shorter than     763 bp
  15% of sequence in fragments shorter than    1051 bp
  20% of sequence in fragments shorter than    1343 bp
  25% of sequence in fragments shorter than    1630 bp
  30% of sequence in fragments shorter than    1936 bp
  35% of sequence in fragments shorter than    2238 bp
  40% of sequence in fragments shorter than    2576 bp
  45% of sequence in fragments shorter than    2940 bp
  50% of sequence in fragments shorter than    3333 bp
  55% of sequence in fragments shorter than    3759 bp
  60% of sequence in fragments shorter than    4234 bp
  65% of sequence in fragments shorter than    4767 bp
  70% of sequence in fragments shorter than    5380 bp
  75% of sequence in fragments shorter than    6092 bp
  80% of sequence in fragments shorter than    6972 bp
  85% of sequence in fragments shorter than    8089 bp
  90% of sequence in fragments shorter than    9651 bp
  95% of sequence in fragments shorter than   12342 bp

# AclI.HhaI (AACGTT+GCGC) double digest, 1928 thousand fragments (172706+1756502 sites), 1545 bp average fragment length
   5% of sequence in fragments shorter than     519 bp
  10% of sequence in fragments shorter than     899 bp
  15% of sequence in fragments shorter than    1270 bp
  20% of sequence in fragments shorter than    1649 bp
  25% of sequence in fragments shorter than    2028 bp
  30% of sequence in fragments shorter than    2430 bp
  35% of sequence in fragments shorter than    2853 bp
  40% of sequence in fragments shorter than    3301 bp
  45% of sequence in fragments shorter than    3783 bp
  50% of sequence in fragments shorter than    4309 bp
  55% of sequence in fragments shorter than    4889 bp
  60% of sequence in fragments shorter than    5539 bp
  65% of sequence in fragments shorter than    6263 bp
  70% of sequence in fragments shorter than    7105 bp
  75% of sequence in fragments shorter than    8092 bp
  80% of sequence in fragments shorter than    9309 bp
  85% of sequence in fragments shorter than   10867 bp
  90% of sequence in fragments shorter than   13083 bp
  95% of sequence in fragments shorter than   17008 bp

# HgiCI.SapI (GGYRCC+GCTCTTC) double digest, 1927 thousand fragments (1550877+377161 sites), 1545 bp average fragment length
   5% of sequence in fragments shorter than     506 bp
  10% of sequence in fragments shorter than     792 bp
  15% of sequence in fragments shorter than    1047 bp
  20% of sequence in fragments shorter than    1268 bp
  25% of sequence in fragments shorter than    1516 bp
  30% of sequence in fragments shorter than    1765 bp
  35% of sequence in fragments shorter than    2027 bp
  40% of sequence in fragments shorter than    2303 bp
  45% of sequence in fragments shorter than    2590 bp
  50% of sequence in fragments shorter than    2900 bp
  55% of sequence in fragments shorter than    3236 bp
  60% of sequence in fragments shorter than    3613 bp
  65% of sequence in fragments shorter than    4038 bp
  70% of sequence in fragments shorter than    4528 bp
  75% of sequence in fragments shorter than    5111 bp
  80% of sequence in fragments shorter than    5827 bp
  85% of sequence in fragments shorter than    6784 bp
  90% of sequence in fragments shorter than    8192 bp
  95% of sequence in fragments shorter than   11162 bp

# HgiCI.SmaI (GGYRCC+CCCGGG) double digest, 1927 thousand fragments (1550877+376940 sites), 1546 bp average fragment length
   5% of sequence in fragments shorter than     511 bp
  10% of sequence in fragments shorter than     823 bp
  15% of sequence in fragments shorter than    1110 bp
  20% of sequence in fragments shorter than    1363 bp
  25% of sequence in fragments shorter than    1633 bp
  30% of sequence in fragments shorter than    1926 bp
  35% of sequence in fragments shorter than    2229 bp
  40% of sequence in fragments shorter than    2549 bp
  45% of sequence in fragments shorter than    2876 bp
  50% of sequence in fragments shorter than    3237 bp
  55% of sequence in fragments shorter than    3642 bp
  60% of sequence in fragments shorter than    4091 bp
  65% of sequence in fragments shorter than    4594 bp
  70% of sequence in fragments shorter than    5188 bp
  75% of sequence in fragments shorter than    5892 bp
  80% of sequence in fragments shorter than    6775 bp
  85% of sequence in fragments shorter than    7944 bp
  90% of sequence in fragments shorter than    9693 bp
  95% of sequence in fragments shorter than   13352 bp

# BciVI.BscGI (GTATCC+CCCGT) double digest, 1926 thousand fragments (696093+1231070 sites), 1546 bp average fragment length
   5% of sequence in fragments shorter than     481 bp
  10% of sequence in fragments shorter than     787 bp
  15% of sequence in fragments shorter than    1059 bp
  20% of sequence in fragments shorter than    1324 bp
  25% of sequence in fragments shorter than    1577 bp
  30% of sequence in fragments shorter than    1829 bp
  35% of sequence in fragments shorter than    2090 bp
  40% of sequence in fragments shorter than    2365 bp
  45% of sequence in fragments shorter than    2655 bp
  50% of sequence in fragments shorter than    2957 bp
  55% of sequence in fragments shorter than    3289 bp
  60% of sequence in fragments shorter than    3648 bp
  65% of sequence in fragments shorter than    4043 bp
  70% of sequence in fragments shorter than    4492 bp
  75% of sequence in fragments shorter than    5019 bp
  80% of sequence in fragments shorter than    5652 bp
  85% of sequence in fragments shorter than    6459 bp
  90% of sequence in fragments shorter than    7570 bp
  95% of sequence in fragments shorter than    9468 bp

# BsmI.SapI (GAATGC+GCTCTTC) double digest, 1926 thousand fragments (1549350+377161 sites), 1547 bp average fragment length
   5% of sequence in fragments shorter than     521 bp
  10% of sequence in fragments shorter than     886 bp
  15% of sequence in fragments shorter than    1188 bp
  20% of sequence in fragments shorter than    1468 bp
  25% of sequence in fragments shorter than    1730 bp
  30% of sequence in fragments shorter than    1999 bp
  35% of sequence in fragments shorter than    2269 bp
  40% of sequence in fragments shorter than    2542 bp
  45% of sequence in fragments shorter than    2831 bp
  50% of sequence in fragments shorter than    3136 bp
  55% of sequence in fragments shorter than    3464 bp
  60% of sequence in fragments shorter than    3816 bp
  65% of sequence in fragments shorter than    4206 bp
  70% of sequence in fragments shorter than    4645 bp
  75% of sequence in fragments shorter than    5148 bp
  80% of sequence in fragments shorter than    5757 bp
  85% of sequence in fragments shorter than    6523 bp
  90% of sequence in fragments shorter than    7586 bp
  95% of sequence in fragments shorter than    9356 bp

# BsmI.SmaI (GAATGC+CCCGGG) double digest, 1925 thousand fragments (1549350+376940 sites), 1547 bp average fragment length
   5% of sequence in fragments shorter than     515 bp
  10% of sequence in fragments shorter than     887 bp
  15% of sequence in fragments shorter than    1186 bp
  20% of sequence in fragments shorter than    1468 bp
  25% of sequence in fragments shorter than    1739 bp
  30% of sequence in fragments shorter than    2012 bp
  35% of sequence in fragments shorter than    2283 bp
  40% of sequence in fragments shorter than    2556 bp
  45% of sequence in fragments shorter than    2839 bp
  50% of sequence in fragments shorter than    3142 bp
  55% of sequence in fragments shorter than    3468 bp
  60% of sequence in fragments shorter than    3823 bp
  65% of sequence in fragments shorter than    4213 bp
  70% of sequence in fragments shorter than    4656 bp
  75% of sequence in fragments shorter than    5166 bp
  80% of sequence in fragments shorter than    5775 bp
  85% of sequence in fragments shorter than    6554 bp
  90% of sequence in fragments shorter than    7607 bp
  95% of sequence in fragments shorter than    9403 bp

# PstI.SacI (CTGCAG+GAGCTC) double digest, 1924 thousand fragments (1321469+603323 sites), 1548 bp average fragment length
   5% of sequence in fragments shorter than     512 bp
  10% of sequence in fragments shorter than     797 bp
  15% of sequence in fragments shorter than    1061 bp
  20% of sequence in fragments shorter than    1316 bp
  25% of sequence in fragments shorter than    1566 bp
  30% of sequence in fragments shorter than    1828 bp
  35% of sequence in fragments shorter than    2092 bp
  40% of sequence in fragments shorter than    2378 bp
  45% of sequence in fragments shorter than    2682 bp
  50% of sequence in fragments shorter than    3005 bp
  55% of sequence in fragments shorter than    3362 bp
  60% of sequence in fragments shorter than    3761 bp
  65% of sequence in fragments shorter than    4209 bp
  70% of sequence in fragments shorter than    4707 bp
  75% of sequence in fragments shorter than    5296 bp
  80% of sequence in fragments shorter than    6008 bp
  85% of sequence in fragments shorter than    6928 bp
  90% of sequence in fragments shorter than    8279 bp
  95% of sequence in fragments shorter than   10776 bp

# BamHI.HgiCI (GGATCC+GGYRCC) double digest, 1916 thousand fragments (365999+1550877 sites), 1554 bp average fragment length
   5% of sequence in fragments shorter than     508 bp
  10% of sequence in fragments shorter than     800 bp
  15% of sequence in fragments shorter than    1068 bp
  20% of sequence in fragments shorter than    1299 bp
  25% of sequence in fragments shorter than    1550 bp
  30% of sequence in fragments shorter than    1799 bp
  35% of sequence in fragments shorter than    2070 bp
  40% of sequence in fragments shorter than    2345 bp
  45% of sequence in fragments shorter than    2648 bp
  50% of sequence in fragments shorter than    2955 bp
  55% of sequence in fragments shorter than    3308 bp
  60% of sequence in fragments shorter than    3701 bp
  65% of sequence in fragments shorter than    4140 bp
  70% of sequence in fragments shorter than    4645 bp
  75% of sequence in fragments shorter than    5258 bp
  80% of sequence in fragments shorter than    6005 bp
  85% of sequence in fragments shorter than    7008 bp
  90% of sequence in fragments shorter than    8497 bp
  95% of sequence in fragments shorter than   11528 bp

# AvrII.PstI (CCTAGG+CTGCAG) double digest, 1915 thousand fragments (594956+1321469 sites), 1555 bp average fragment length
   5% of sequence in fragments shorter than     532 bp
  10% of sequence in fragments shorter than     803 bp
  15% of sequence in fragments shorter than    1067 bp
  20% of sequence in fragments shorter than    1316 bp
  25% of sequence in fragments shorter than    1559 bp
  30% of sequence in fragments shorter than    1810 bp
  35% of sequence in fragments shorter than    2066 bp
  40% of sequence in fragments shorter than    2327 bp
  45% of sequence in fragments shorter than    2608 bp
  50% of sequence in fragments shorter than    2892 bp
  55% of sequence in fragments shorter than    3206 bp
  60% of sequence in fragments shorter than    3551 bp
  65% of sequence in fragments shorter than    3937 bp
  70% of sequence in fragments shorter than    4388 bp
  75% of sequence in fragments shorter than    4922 bp
  80% of sequence in fragments shorter than    5580 bp
  85% of sequence in fragments shorter than    6433 bp
  90% of sequence in fragments shorter than    7682 bp
  95% of sequence in fragments shorter than    9944 bp

# HhaI.PacI (GCGC+TTAATTAA) double digest, 1915 thousand fragments (1756502+159455 sites), 1555 bp average fragment length
   5% of sequence in fragments shorter than     528 bp
  10% of sequence in fragments shorter than     916 bp
  15% of sequence in fragments shorter than    1293 bp
  20% of sequence in fragments shorter than    1672 bp
  25% of sequence in fragments shorter than    2050 bp
  30% of sequence in fragments shorter than    2454 bp
  35% of sequence in fragments shorter than    2875 bp
  40% of sequence in fragments shorter than    3324 bp
  45% of sequence in fragments shorter than    3802 bp
  50% of sequence in fragments shorter than    4314 bp
  55% of sequence in fragments shorter than    4889 bp
  60% of sequence in fragments shorter than    5525 bp
  65% of sequence in fragments shorter than    6222 bp
  70% of sequence in fragments shorter than    7036 bp
  75% of sequence in fragments shorter than    8000 bp
  80% of sequence in fragments shorter than    9168 bp
  85% of sequence in fragments shorter than   10671 bp
  90% of sequence in fragments shorter than   12795 bp
  95% of sequence in fragments shorter than   16498 bp

# BamHI.BsmI (GGATCC+GAATGC) double digest, 1914 thousand fragments (365999+1549350 sites), 1556 bp average fragment length
   5% of sequence in fragments shorter than     526 bp
  10% of sequence in fragments shorter than     890 bp
  15% of sequence in fragments shorter than    1192 bp
  20% of sequence in fragments shorter than    1477 bp
  25% of sequence in fragments shorter than    1748 bp
  30% of sequence in fragments shorter than    2019 bp
  35% of sequence in fragments shorter than    2291 bp
  40% of sequence in fragments shorter than    2562 bp
  45% of sequence in fragments shorter than    2845 bp
  50% of sequence in fragments shorter than    3146 bp
  55% of sequence in fragments shorter than    3472 bp
  60% of sequence in fragments shorter than    3823 bp
  65% of sequence in fragments shorter than    4212 bp
  70% of sequence in fragments shorter than    4652 bp
  75% of sequence in fragments shorter than    5156 bp
  80% of sequence in fragments shorter than    5765 bp
  85% of sequence in fragments shorter than    6531 bp
  90% of sequence in fragments shorter than    7579 bp
  95% of sequence in fragments shorter than    9352 bp

# HhaI.Hpy99I (GCGC+CGWCG) double digest, 1913 thousand fragments (1756502+157917 sites), 1556 bp average fragment length
   5% of sequence in fragments shorter than     520 bp
  10% of sequence in fragments shorter than     924 bp
  15% of sequence in fragments shorter than    1332 bp
  20% of sequence in fragments shorter than    1746 bp
  25% of sequence in fragments shorter than    2172 bp
  30% of sequence in fragments shorter than    2632 bp
  35% of sequence in fragments shorter than    3122 bp
  40% of sequence in fragments shorter than    3647 bp
  45% of sequence in fragments shorter than    4212 bp
  50% of sequence in fragments shorter than    4846 bp
  55% of sequence in fragments shorter than    5546 bp
  60% of sequence in fragments shorter than    6315 bp
  65% of sequence in fragments shorter than    7204 bp
  70% of sequence in fragments shorter than    8224 bp
  75% of sequence in fragments shorter than    9439 bp
  80% of sequence in fragments shorter than   10941 bp
  85% of sequence in fragments shorter than   12894 bp
  90% of sequence in fragments shorter than   15692 bp
  95% of sequence in fragments shorter than   20511 bp

# AvaIII.BspHI (ATGCAT+TCATGA) double digest, 1911 thousand fragments (933357+978290 sites), 1559 bp average fragment length
   5% of sequence in fragments shorter than     550 bp
  10% of sequence in fragments shorter than     828 bp
  15% of sequence in fragments shorter than    1075 bp
  20% of sequence in fragments shorter than    1307 bp
  25% of sequence in fragments shorter than    1535 bp
  30% of sequence in fragments shorter than    1765 bp
  35% of sequence in fragments shorter than    1993 bp
  40% of sequence in fragments shorter than    2227 bp
  45% of sequence in fragments shorter than    2475 bp
  50% of sequence in fragments shorter than    2744 bp
  55% of sequence in fragments shorter than    3030 bp
  60% of sequence in fragments shorter than    3346 bp
  65% of sequence in fragments shorter than    3703 bp
  70% of sequence in fragments shorter than    4118 bp
  75% of sequence in fragments shorter than    4604 bp
  80% of sequence in fragments shorter than    5189 bp
  85% of sequence in fragments shorter than    5977 bp
  90% of sequence in fragments shorter than    7143 bp
  95% of sequence in fragments shorter than    9459 bp

# AvaI.NdeI (CYCGRG+CATATG) double digest, 1908 thousand fragments (1005714+903360 sites), 1561 bp average fragment length
   5% of sequence in fragments shorter than     534 bp
  10% of sequence in fragments shorter than     827 bp
  15% of sequence in fragments shorter than    1086 bp
  20% of sequence in fragments shorter than    1321 bp
  25% of sequence in fragments shorter than    1565 bp
  30% of sequence in fragments shorter than    1812 bp
  35% of sequence in fragments shorter than    2064 bp
  40% of sequence in fragments shorter than    2319 bp
  45% of sequence in fragments shorter than    2595 bp
  50% of sequence in fragments shorter than    2885 bp
  55% of sequence in fragments shorter than    3180 bp
  60% of sequence in fragments shorter than    3515 bp
  65% of sequence in fragments shorter than    3892 bp
  70% of sequence in fragments shorter than    4321 bp
  75% of sequence in fragments shorter than    4821 bp
  80% of sequence in fragments shorter than    5431 bp
  85% of sequence in fragments shorter than    6234 bp
  90% of sequence in fragments shorter than    7387 bp
  95% of sequence in fragments shorter than    9677 bp

# BspHI.StuI (TCATGA+AGGCCT) double digest, 1903 thousand fragments (978290+925729 sites), 1565 bp average fragment length
   5% of sequence in fragments shorter than     518 bp
  10% of sequence in fragments shorter than     802 bp
  15% of sequence in fragments shorter than    1070 bp
  20% of sequence in fragments shorter than    1327 bp
  25% of sequence in fragments shorter than    1560 bp
  30% of sequence in fragments shorter than    1808 bp
  35% of sequence in fragments shorter than    2038 bp
  40% of sequence in fragments shorter than    2293 bp
  45% of sequence in fragments shorter than    2551 bp
  50% of sequence in fragments shorter than    2831 bp
  55% of sequence in fragments shorter than    3129 bp
  60% of sequence in fragments shorter than    3452 bp
  65% of sequence in fragments shorter than    3808 bp
  70% of sequence in fragments shorter than    4205 bp
  75% of sequence in fragments shorter than    4671 bp
  80% of sequence in fragments shorter than    5224 bp
  85% of sequence in fragments shorter than    5926 bp
  90% of sequence in fragments shorter than    6895 bp
  95% of sequence in fragments shorter than    8527 bp

# EsaBC3I.SexAI (TCGA+ACCWGGT) double digest, 1900 thousand fragments (1603346+297900 sites), 1567 bp average fragment length
   5% of sequence in fragments shorter than     535 bp
  10% of sequence in fragments shorter than     822 bp
  15% of sequence in fragments shorter than    1070 bp
  20% of sequence in fragments shorter than    1307 bp
  25% of sequence in fragments shorter than    1532 bp
  30% of sequence in fragments shorter than    1769 bp
  35% of sequence in fragments shorter than    2012 bp
  40% of sequence in fragments shorter than    2265 bp
  45% of sequence in fragments shorter than    2532 bp
  50% of sequence in fragments shorter than    2814 bp
  55% of sequence in fragments shorter than    3113 bp
  60% of sequence in fragments shorter than    3445 bp
  65% of sequence in fragments shorter than    3816 bp
  70% of sequence in fragments shorter than    4238 bp
  75% of sequence in fragments shorter than    4734 bp
  80% of sequence in fragments shorter than    5333 bp
  85% of sequence in fragments shorter than    6094 bp
  90% of sequence in fragments shorter than    7179 bp
  95% of sequence in fragments shorter than    8995 bp

# ApaI.FauI (GGGCCC+CCCGC) double digest, 1899 thousand fragments (460339+1439772 sites), 1568 bp average fragment length
   5% of sequence in fragments shorter than     518 bp
  10% of sequence in fragments shorter than     929 bp
  15% of sequence in fragments shorter than    1331 bp
  20% of sequence in fragments shorter than    1725 bp
  25% of sequence in fragments shorter than    2137 bp
  30% of sequence in fragments shorter than    2584 bp
  35% of sequence in fragments shorter than    3056 bp
  40% of sequence in fragments shorter than    3565 bp
  45% of sequence in fragments shorter than    4114 bp
  50% of sequence in fragments shorter than    4689 bp
  55% of sequence in fragments shorter than    5357 bp
  60% of sequence in fragments shorter than    6106 bp
  65% of sequence in fragments shorter than    6959 bp
  70% of sequence in fragments shorter than    7956 bp
  75% of sequence in fragments shorter than    9112 bp
  80% of sequence in fragments shorter than   10563 bp
  85% of sequence in fragments shorter than   12467 bp
  90% of sequence in fragments shorter than   15250 bp
  95% of sequence in fragments shorter than   20658 bp

# ApaI.BspMI (GGGCCC+ACCTGC) double digest, 1898 thousand fragments (460339+1438129 sites), 1570 bp average fragment length
   5% of sequence in fragments shorter than     506 bp
  10% of sequence in fragments shorter than     816 bp
  15% of sequence in fragments shorter than    1089 bp
  20% of sequence in fragments shorter than    1367 bp
  25% of sequence in fragments shorter than    1636 bp
  30% of sequence in fragments shorter than    1921 bp
  35% of sequence in fragments shorter than    2188 bp
  40% of sequence in fragments shorter than    2492 bp
  45% of sequence in fragments shorter than    2811 bp
  50% of sequence in fragments shorter than    3162 bp
  55% of sequence in fragments shorter than    3486 bp
  60% of sequence in fragments shorter than    3893 bp
  65% of sequence in fragments shorter than    4353 bp
  70% of sequence in fragments shorter than    4877 bp
  75% of sequence in fragments shorter than    5479 bp
  80% of sequence in fragments shorter than    6215 bp
  85% of sequence in fragments shorter than    7174 bp
  90% of sequence in fragments shorter than    8521 bp
  95% of sequence in fragments shorter than   10876 bp

# HgaI.PstI (GACGC+CTGCAG) double digest, 1892 thousand fragments (571889+1321469 sites), 1574 bp average fragment length
   5% of sequence in fragments shorter than     512 bp
  10% of sequence in fragments shorter than     815 bp
  15% of sequence in fragments shorter than    1102 bp
  20% of sequence in fragments shorter than    1379 bp
  25% of sequence in fragments shorter than    1655 bp
  30% of sequence in fragments shorter than    1936 bp
  35% of sequence in fragments shorter than    2232 bp
  40% of sequence in fragments shorter than    2545 bp
  45% of sequence in fragments shorter than    2879 bp
  50% of sequence in fragments shorter than    3244 bp
  55% of sequence in fragments shorter than    3637 bp
  60% of sequence in fragments shorter than    4072 bp
  65% of sequence in fragments shorter than    4562 bp
  70% of sequence in fragments shorter than    5119 bp
  75% of sequence in fragments shorter than    5773 bp
  80% of sequence in fragments shorter than    6569 bp
  85% of sequence in fragments shorter than    7602 bp
  90% of sequence in fragments shorter than    9083 bp
  95% of sequence in fragments shorter than   11778 bp

# EciI.PstI (GGCGGA+CTGCAG) double digest, 1892 thousand fragments (571273+1321469 sites), 1574 bp average fragment length
   5% of sequence in fragments shorter than     510 bp
  10% of sequence in fragments shorter than     796 bp
  15% of sequence in fragments shorter than    1064 bp
  20% of sequence in fragments shorter than    1325 bp
  25% of sequence in fragments shorter than    1586 bp
  30% of sequence in fragments shorter than    1864 bp
  35% of sequence in fragments shorter than    2142 bp
  40% of sequence in fragments shorter than    2445 bp
  45% of sequence in fragments shorter than    2770 bp
  50% of sequence in fragments shorter than    3129 bp
  55% of sequence in fragments shorter than    3523 bp
  60% of sequence in fragments shorter than    3965 bp
  65% of sequence in fragments shorter than    4470 bp
  70% of sequence in fragments shorter than    5042 bp
  75% of sequence in fragments shorter than    5709 bp
  80% of sequence in fragments shorter than    6516 bp
  85% of sequence in fragments shorter than    7594 bp
  90% of sequence in fragments shorter than    9155 bp
  95% of sequence in fragments shorter than   12053 bp

# EsaBC3I.KpnI (TCGA+GGTACC) double digest, 1890 thousand fragments (1603346+287974 sites), 1576 bp average fragment length
   5% of sequence in fragments shorter than     539 bp
  10% of sequence in fragments shorter than     830 bp
  15% of sequence in fragments shorter than    1078 bp
  20% of sequence in fragments shorter than    1309 bp
  25% of sequence in fragments shorter than    1535 bp
  30% of sequence in fragments shorter than    1769 bp
  35% of sequence in fragments shorter than    2013 bp
  40% of sequence in fragments shorter than    2269 bp
  45% of sequence in fragments shorter than    2536 bp
  50% of sequence in fragments shorter than    2817 bp
  55% of sequence in fragments shorter than    3118 bp
  60% of sequence in fragments shorter than    3449 bp
  65% of sequence in fragments shorter than    3819 bp
  70% of sequence in fragments shorter than    4239 bp
  75% of sequence in fragments shorter than    4738 bp
  80% of sequence in fragments shorter than    5334 bp
  85% of sequence in fragments shorter than    6099 bp
  90% of sequence in fragments shorter than    7171 bp
  95% of sequence in fragments shorter than    8995 bp

# EsaBC3I.NheI (TCGA+GCTAGC) double digest, 1890 thousand fragments (1603346+287384 sites), 1576 bp average fragment length
   5% of sequence in fragments shorter than     541 bp
  10% of sequence in fragments shorter than     833 bp
  15% of sequence in fragments shorter than    1078 bp
  20% of sequence in fragments shorter than    1316 bp
  25% of sequence in fragments shorter than    1542 bp
  30% of sequence in fragments shorter than    1771 bp
  35% of sequence in fragments shorter than    2012 bp
  40% of sequence in fragments shorter than    2264 bp
  45% of sequence in fragments shorter than    2532 bp
  50% of sequence in fragments shorter than    2814 bp
  55% of sequence in fragments shorter than    3114 bp
  60% of sequence in fragments shorter than    3445 bp
  65% of sequence in fragments shorter than    3815 bp
  70% of sequence in fragments shorter than    4233 bp
  75% of sequence in fragments shorter than    4727 bp
  80% of sequence in fragments shorter than    5317 bp
  85% of sequence in fragments shorter than    6069 bp
  90% of sequence in fragments shorter than    7127 bp
  95% of sequence in fragments shorter than    8922 bp

# HhaI.SnaBI (GCGC+TACGTA) double digest, 1888 thousand fragments (1756502+132299 sites), 1578 bp average fragment length
   5% of sequence in fragments shorter than     526 bp
  10% of sequence in fragments shorter than     924 bp
  15% of sequence in fragments shorter than    1313 bp
  20% of sequence in fragments shorter than    1705 bp
  25% of sequence in fragments shorter than    2093 bp
  30% of sequence in fragments shorter than    2511 bp
  35% of sequence in fragments shorter than    2949 bp
  40% of sequence in fragments shorter than    3418 bp
  45% of sequence in fragments shorter than    3922 bp
  50% of sequence in fragments shorter than    4468 bp
  55% of sequence in fragments shorter than    5069 bp
  60% of sequence in fragments shorter than    5742 bp
  65% of sequence in fragments shorter than    6492 bp
  70% of sequence in fragments shorter than    7356 bp
  75% of sequence in fragments shorter than    8387 bp
  80% of sequence in fragments shorter than    9647 bp
  85% of sequence in fragments shorter than   11277 bp
  90% of sequence in fragments shorter than   13583 bp
  95% of sequence in fragments shorter than   17677 bp

# BstEII.HgiCI (GGTNACC+GGYRCC) double digest, 1886 thousand fragments (335865+1550877 sites), 1579 bp average fragment length
   5% of sequence in fragments shorter than     516 bp
  10% of sequence in fragments shorter than     810 bp
  15% of sequence in fragments shorter than    1072 bp
  20% of sequence in fragments shorter than    1311 bp
  25% of sequence in fragments shorter than    1569 bp
  30% of sequence in fragments shorter than    1819 bp
  35% of sequence in fragments shorter than    2084 bp
  40% of sequence in fragments shorter than    2370 bp
  45% of sequence in fragments shorter than    2675 bp
  50% of sequence in fragments shorter than    2995 bp
  55% of sequence in fragments shorter than    3345 bp
  60% of sequence in fragments shorter than    3742 bp
  65% of sequence in fragments shorter than    4188 bp
  70% of sequence in fragments shorter than    4704 bp
  75% of sequence in fragments shorter than    5313 bp
  80% of sequence in fragments shorter than    6058 bp
  85% of sequence in fragments shorter than    7073 bp
  90% of sequence in fragments shorter than    8588 bp
  95% of sequence in fragments shorter than   11776 bp

# EcoRV.FauI (GATATC+CCCGC) double digest, 1885 thousand fragments (446473+1439772 sites), 1580 bp average fragment length
   5% of sequence in fragments shorter than     556 bp
  10% of sequence in fragments shorter than     933 bp
  15% of sequence in fragments shorter than    1277 bp
  20% of sequence in fragments shorter than    1587 bp
  25% of sequence in fragments shorter than    1909 bp
  30% of sequence in fragments shorter than    2231 bp
  35% of sequence in fragments shorter than    2572 bp
  40% of sequence in fragments shorter than    2911 bp
  45% of sequence in fragments shorter than    3283 bp
  50% of sequence in fragments shorter than    3685 bp
  55% of sequence in fragments shorter than    4121 bp
  60% of sequence in fragments shorter than    4594 bp
  65% of sequence in fragments shorter than    5132 bp
  70% of sequence in fragments shorter than    5735 bp
  75% of sequence in fragments shorter than    6448 bp
  80% of sequence in fragments shorter than    7291 bp
  85% of sequence in fragments shorter than    8395 bp
  90% of sequence in fragments shorter than    9969 bp
  95% of sequence in fragments shorter than   12762 bp

# MfeI.PstI (CAATTG+CTGCAG) double digest, 1885 thousand fragments (564303+1321469 sites), 1580 bp average fragment length
   5% of sequence in fragments shorter than     550 bp
  10% of sequence in fragments shorter than     830 bp
  15% of sequence in fragments shorter than    1080 bp
  20% of sequence in fragments shorter than    1321 bp
  25% of sequence in fragments shorter than    1553 bp
  30% of sequence in fragments shorter than    1790 bp
  35% of sequence in fragments shorter than    2025 bp
  40% of sequence in fragments shorter than    2262 bp
  45% of sequence in fragments shorter than    2522 bp
  50% of sequence in fragments shorter than    2789 bp
  55% of sequence in fragments shorter than    3061 bp
  60% of sequence in fragments shorter than    3379 bp
  65% of sequence in fragments shorter than    3721 bp
  70% of sequence in fragments shorter than    4118 bp
  75% of sequence in fragments shorter than    4580 bp
  80% of sequence in fragments shorter than    5133 bp
  85% of sequence in fragments shorter than    5846 bp
  90% of sequence in fragments shorter than    6840 bp
  95% of sequence in fragments shorter than    8563 bp

# AflIII.SpeI (ACRYGT+ACTAGT) double digest, 1885 thousand fragments (1485394+400286 sites), 1580 bp average fragment length
   5% of sequence in fragments shorter than     554 bp
  10% of sequence in fragments shorter than     869 bp
  15% of sequence in fragments shorter than    1141 bp
  20% of sequence in fragments shorter than    1391 bp
  25% of sequence in fragments shorter than    1639 bp
  30% of sequence in fragments shorter than    1885 bp
  35% of sequence in fragments shorter than    2125 bp
  40% of sequence in fragments shorter than    2382 bp
  45% of sequence in fragments shorter than    2650 bp
  50% of sequence in fragments shorter than    2930 bp
  55% of sequence in fragments shorter than    3230 bp
  60% of sequence in fragments shorter than    3567 bp
  65% of sequence in fragments shorter than    3940 bp
  70% of sequence in fragments shorter than    4367 bp
  75% of sequence in fragments shorter than    4869 bp
  80% of sequence in fragments shorter than    5482 bp
  85% of sequence in fragments shorter than    6233 bp
  90% of sequence in fragments shorter than    7303 bp
  95% of sequence in fragments shorter than    9283 bp

# BsmI.BstEII (GAATGC+GGTNACC) double digest, 1884 thousand fragments (1549350+335865 sites), 1581 bp average fragment length
   5% of sequence in fragments shorter than     530 bp
  10% of sequence in fragments shorter than     914 bp
  15% of sequence in fragments shorter than    1221 bp
  20% of sequence in fragments shorter than    1510 bp
  25% of sequence in fragments shorter than    1791 bp
  30% of sequence in fragments shorter than    2065 bp
  35% of sequence in fragments shorter than    2334 bp
  40% of sequence in fragments shorter than    2598 bp
  45% of sequence in fragments shorter than    2890 bp
  50% of sequence in fragments shorter than    3196 bp
  55% of sequence in fragments shorter than    3527 bp
  60% of sequence in fragments shorter than    3887 bp
  65% of sequence in fragments shorter than    4284 bp
  70% of sequence in fragments shorter than    4727 bp
  75% of sequence in fragments shorter than    5238 bp
  80% of sequence in fragments shorter than    5860 bp
  85% of sequence in fragments shorter than    6626 bp
  90% of sequence in fragments shorter than    7681 bp
  95% of sequence in fragments shorter than    9487 bp

# BspMI.EcoRV (ACCTGC+GATATC) double digest, 1884 thousand fragments (1438129+446473 sites), 1581 bp average fragment length
   5% of sequence in fragments shorter than     532 bp
  10% of sequence in fragments shorter than     830 bp
  15% of sequence in fragments shorter than    1083 bp
  20% of sequence in fragments shorter than    1328 bp
  25% of sequence in fragments shorter than    1560 bp
  30% of sequence in fragments shorter than    1794 bp
  35% of sequence in fragments shorter than    2026 bp
  40% of sequence in fragments shorter than    2273 bp
  45% of sequence in fragments shorter than    2535 bp
  50% of sequence in fragments shorter than    2808 bp
  55% of sequence in fragments shorter than    3077 bp
  60% of sequence in fragments shorter than    3391 bp
  65% of sequence in fragments shorter than    3748 bp
  70% of sequence in fragments shorter than    4151 bp
  75% of sequence in fragments shorter than    4619 bp
  80% of sequence in fragments shorter than    5179 bp
  85% of sequence in fragments shorter than    5892 bp
  90% of sequence in fragments shorter than    6894 bp
  95% of sequence in fragments shorter than    8604 bp

# BsrBI.PsiI (CCGCTC+TTATAA) double digest, 1882 thousand fragments (235523+1647912 sites), 1582 bp average fragment length
   5% of sequence in fragments shorter than     541 bp
  10% of sequence in fragments shorter than     841 bp
  15% of sequence in fragments shorter than    1103 bp
  20% of sequence in fragments shorter than    1353 bp
  25% of sequence in fragments shorter than    1598 bp
  30% of sequence in fragments shorter than    1847 bp
  35% of sequence in fragments shorter than    2098 bp
  40% of sequence in fragments shorter than    2367 bp
  45% of sequence in fragments shorter than    2651 bp
  50% of sequence in fragments shorter than    2937 bp
  55% of sequence in fragments shorter than    3259 bp
  60% of sequence in fragments shorter than    3614 bp
  65% of sequence in fragments shorter than    4012 bp
  70% of sequence in fragments shorter than    4473 bp
  75% of sequence in fragments shorter than    5019 bp
  80% of sequence in fragments shorter than    5697 bp
  85% of sequence in fragments shorter than    6584 bp
  90% of sequence in fragments shorter than    7897 bp
  95% of sequence in fragments shorter than   10496 bp

# HhaI.NaeI (GCGC+GCCGGC) double digest, 1881 thousand fragments (1756502+125692 sites), 1583 bp average fragment length
   5% of sequence in fragments shorter than     530 bp
  10% of sequence in fragments shorter than     942 bp
  15% of sequence in fragments shorter than    1362 bp
  20% of sequence in fragments shorter than    1775 bp
  25% of sequence in fragments shorter than    2222 bp
  30% of sequence in fragments shorter than    2694 bp
  35% of sequence in fragments shorter than    3203 bp
  40% of sequence in fragments shorter than    3750 bp
  45% of sequence in fragments shorter than    4336 bp
  50% of sequence in fragments shorter than    5001 bp
  55% of sequence in fragments shorter than    5716 bp
  60% of sequence in fragments shorter than    6515 bp
  65% of sequence in fragments shorter than    7435 bp
  70% of sequence in fragments shorter than    8506 bp
  75% of sequence in fragments shorter than    9786 bp
  80% of sequence in fragments shorter than   11353 bp
  85% of sequence in fragments shorter than   13395 bp
  90% of sequence in fragments shorter than   16265 bp
  95% of sequence in fragments shorter than   21362 bp

# BspHI.NdeI (TCATGA+CATATG) double digest, 1881 thousand fragments (978290+903360 sites), 1584 bp average fragment length
   5% of sequence in fragments shorter than     538 bp
  10% of sequence in fragments shorter than     828 bp
  15% of sequence in fragments shorter than    1082 bp
  20% of sequence in fragments shorter than    1312 bp
  25% of sequence in fragments shorter than    1550 bp
  30% of sequence in fragments shorter than    1790 bp
  35% of sequence in fragments shorter than    2031 bp
  40% of sequence in fragments shorter than    2278 bp
  45% of sequence in fragments shorter than    2547 bp
  50% of sequence in fragments shorter than    2833 bp
  55% of sequence in fragments shorter than    3145 bp
  60% of sequence in fragments shorter than    3491 bp
  65% of sequence in fragments shorter than    3881 bp
  70% of sequence in fragments shorter than    4325 bp
  75% of sequence in fragments shorter than    4838 bp
  80% of sequence in fragments shorter than    5471 bp
  85% of sequence in fragments shorter than    6321 bp
  90% of sequence in fragments shorter than    7627 bp
  95% of sequence in fragments shorter than   10407 bp

# AclI.BfiI (AACGTT+ACTGGG) double digest, 1878 thousand fragments (172706+1706594 sites), 1586 bp average fragment length
   5% of sequence in fragments shorter than     543 bp
  10% of sequence in fragments shorter than     832 bp
  15% of sequence in fragments shorter than    1082 bp
  20% of sequence in fragments shorter than    1324 bp
  25% of sequence in fragments shorter than    1564 bp
  30% of sequence in fragments shorter than    1801 bp
  35% of sequence in fragments shorter than    2045 bp
  40% of sequence in fragments shorter than    2274 bp
  45% of sequence in fragments shorter than    2540 bp
  50% of sequence in fragments shorter than    2805 bp
  55% of sequence in fragments shorter than    3110 bp
  60% of sequence in fragments shorter than    3443 bp
  65% of sequence in fragments shorter than    3813 bp
  70% of sequence in fragments shorter than    4232 bp
  75% of sequence in fragments shorter than    4723 bp
  80% of sequence in fragments shorter than    5308 bp
  85% of sequence in fragments shorter than    6069 bp
  90% of sequence in fragments shorter than    7173 bp
  95% of sequence in fragments shorter than    9248 bp

# KasI.PsiI (GGCGCC+TTATAA) double digest, 1877 thousand fragments (230381+1647912 sites), 1586 bp average fragment length
   5% of sequence in fragments shorter than     537 bp
  10% of sequence in fragments shorter than     833 bp
  15% of sequence in fragments shorter than    1094 bp
  20% of sequence in fragments shorter than    1343 bp
  25% of sequence in fragments shorter than    1589 bp
  30% of sequence in fragments shorter than    1839 bp
  35% of sequence in fragments shorter than    2094 bp
  40% of sequence in fragments shorter than    2357 bp
  45% of sequence in fragments shorter than    2640 bp
  50% of sequence in fragments shorter than    2934 bp
  55% of sequence in fragments shorter than    3260 bp
  60% of sequence in fragments shorter than    3627 bp
  65% of sequence in fragments shorter than    4033 bp
  70% of sequence in fragments shorter than    4510 bp
  75% of sequence in fragments shorter than    5084 bp
  80% of sequence in fragments shorter than    5796 bp
  85% of sequence in fragments shorter than    6749 bp
  90% of sequence in fragments shorter than    8195 bp
  95% of sequence in fragments shorter than   11237 bp

# HhaI.XhoI (GCGC+CTCGAG) double digest, 1877 thousand fragments (1756502+121308 sites), 1587 bp average fragment length
   5% of sequence in fragments shorter than     524 bp
  10% of sequence in fragments shorter than     923 bp
  15% of sequence in fragments shorter than    1322 bp
  20% of sequence in fragments shorter than    1727 bp
  25% of sequence in fragments shorter than    2140 bp
  30% of sequence in fragments shorter than    2589 bp
  35% of sequence in fragments shorter than    3064 bp
  40% of sequence in fragments shorter than    3573 bp
  45% of sequence in fragments shorter than    4131 bp
  50% of sequence in fragments shorter than    4728 bp
  55% of sequence in fragments shorter than    5402 bp
  60% of sequence in fragments shorter than    6152 bp
  65% of sequence in fragments shorter than    7002 bp
  70% of sequence in fragments shorter than    7995 bp
  75% of sequence in fragments shorter than    9161 bp
  80% of sequence in fragments shorter than   10614 bp
  85% of sequence in fragments shorter than   12491 bp
  90% of sequence in fragments shorter than   15145 bp
  95% of sequence in fragments shorter than   19756 bp

# AccI.BglII (GTMKAC+AGATCT) double digest, 1875 thousand fragments (1101063+774732 sites), 1589 bp average fragment length
   5% of sequence in fragments shorter than     558 bp
  10% of sequence in fragments shorter than     838 bp
  15% of sequence in fragments shorter than    1086 bp
  20% of sequence in fragments shorter than    1314 bp
  25% of sequence in fragments shorter than    1531 bp
  30% of sequence in fragments shorter than    1751 bp
  35% of sequence in fragments shorter than    1973 bp
  40% of sequence in fragments shorter than    2211 bp
  45% of sequence in fragments shorter than    2455 bp
  50% of sequence in fragments shorter than    2714 bp
  55% of sequence in fragments shorter than    2994 bp
  60% of sequence in fragments shorter than    3290 bp
  65% of sequence in fragments shorter than    3627 bp
  70% of sequence in fragments shorter than    3988 bp
  75% of sequence in fragments shorter than    4439 bp
  80% of sequence in fragments shorter than    4982 bp
  85% of sequence in fragments shorter than    5692 bp
  90% of sequence in fragments shorter than    6729 bp
  95% of sequence in fragments shorter than    8662 bp

# AflIII.HpaI (ACRYGT+GTTAAC) double digest, 1874 thousand fragments (1485394+389418 sites), 1589 bp average fragment length
   5% of sequence in fragments shorter than     566 bp
  10% of sequence in fragments shorter than     880 bp
  15% of sequence in fragments shorter than    1152 bp
  20% of sequence in fragments shorter than    1404 bp
  25% of sequence in fragments shorter than    1652 bp
  30% of sequence in fragments shorter than    1897 bp
  35% of sequence in fragments shorter than    2139 bp
  40% of sequence in fragments shorter than    2395 bp
  45% of sequence in fragments shorter than    2664 bp
  50% of sequence in fragments shorter than    2937 bp
  55% of sequence in fragments shorter than    3227 bp
  60% of sequence in fragments shorter than    3556 bp
  65% of sequence in fragments shorter than    3925 bp
  70% of sequence in fragments shorter than    4345 bp
  75% of sequence in fragments shorter than    4841 bp
  80% of sequence in fragments shorter than    5448 bp
  85% of sequence in fragments shorter than    6213 bp
  90% of sequence in fragments shorter than    7298 bp
  95% of sequence in fragments shorter than    9355 bp

# PsiI.SwaI (TTATAA+ATTTAAAT) double digest, 1874 thousand fragments (1647912+226602 sites), 1590 bp average fragment length
   5% of sequence in fragments shorter than     526 bp
  10% of sequence in fragments shorter than     823 bp
  15% of sequence in fragments shorter than    1089 bp
  20% of sequence in fragments shorter than    1348 bp
  25% of sequence in fragments shorter than    1607 bp
  30% of sequence in fragments shorter than    1873 bp
  35% of sequence in fragments shorter than    2151 bp
  40% of sequence in fragments shorter than    2450 bp
  45% of sequence in fragments shorter than    2772 bp
  50% of sequence in fragments shorter than    3101 bp
  55% of sequence in fragments shorter than    3481 bp
  60% of sequence in fragments shorter than    3914 bp
  65% of sequence in fragments shorter than    4422 bp
  70% of sequence in fragments shorter than    5035 bp
  75% of sequence in fragments shorter than    5808 bp
  80% of sequence in fragments shorter than    6832 bp
  85% of sequence in fragments shorter than    8315 bp
  90% of sequence in fragments shorter than   10938 bp
  95% of sequence in fragments shorter than   18204 bp

# AflII.BscGI (CTTAAG+CCCGT) double digest, 1873 thousand fragments (643284+1231070 sites), 1590 bp average fragment length
   5% of sequence in fragments shorter than     493 bp
  10% of sequence in fragments shorter than     801 bp
  15% of sequence in fragments shorter than    1080 bp
  20% of sequence in fragments shorter than    1352 bp
  25% of sequence in fragments shorter than    1615 bp
  30% of sequence in fragments shorter than    1879 bp
  35% of sequence in fragments shorter than    2148 bp
  40% of sequence in fragments shorter than    2427 bp
  45% of sequence in fragments shorter than    2720 bp
  50% of sequence in fragments shorter than    3024 bp
  55% of sequence in fragments shorter than    3356 bp
  60% of sequence in fragments shorter than    3715 bp
  65% of sequence in fragments shorter than    4118 bp
  70% of sequence in fragments shorter than    4573 bp
  75% of sequence in fragments shorter than    5108 bp
  80% of sequence in fragments shorter than    5746 bp
  85% of sequence in fragments shorter than    6558 bp
  90% of sequence in fragments shorter than    7725 bp
  95% of sequence in fragments shorter than    9646 bp

# PstI.SphI (CTGCAG+GCATGC) double digest, 1871 thousand fragments (1321469+550952 sites), 1591 bp average fragment length
   5% of sequence in fragments shorter than     537 bp
  10% of sequence in fragments shorter than     825 bp
  15% of sequence in fragments shorter than    1089 bp
  20% of sequence in fragments shorter than    1341 bp
  25% of sequence in fragments shorter than    1587 bp
  30% of sequence in fragments shorter than    1845 bp
  35% of sequence in fragments shorter than    2103 bp
  40% of sequence in fragments shorter than    2381 bp
  45% of sequence in fragments shorter than    2676 bp
  50% of sequence in fragments shorter than    2987 bp
  55% of sequence in fragments shorter than    3335 bp
  60% of sequence in fragments shorter than    3718 bp
  65% of sequence in fragments shorter than    4147 bp
  70% of sequence in fragments shorter than    4643 bp
  75% of sequence in fragments shorter than    5211 bp
  80% of sequence in fragments shorter than    5896 bp
  85% of sequence in fragments shorter than    6768 bp
  90% of sequence in fragments shorter than    8013 bp
  95% of sequence in fragments shorter than   10369 bp

# BfiI.PacI (ACTGGG+TTAATTAA) double digest, 1865 thousand fragments (1706594+159455 sites), 1597 bp average fragment length
   5% of sequence in fragments shorter than     548 bp
  10% of sequence in fragments shorter than     845 bp
  15% of sequence in fragments shorter than    1097 bp
  20% of sequence in fragments shorter than    1343 bp
  25% of sequence in fragments shorter than    1582 bp
  30% of sequence in fragments shorter than    1820 bp
  35% of sequence in fragments shorter than    2064 bp
  40% of sequence in fragments shorter than    2297 bp
  45% of sequence in fragments shorter than    2563 bp
  50% of sequence in fragments shorter than    2817 bp
  55% of sequence in fragments shorter than    3122 bp
  60% of sequence in fragments shorter than    3452 bp
  65% of sequence in fragments shorter than    3822 bp
  70% of sequence in fragments shorter than    4239 bp
  75% of sequence in fragments shorter than    4725 bp
  80% of sequence in fragments shorter than    5303 bp
  85% of sequence in fragments shorter than    6063 bp
  90% of sequence in fragments shorter than    7177 bp
  95% of sequence in fragments shorter than    9355 bp

# AvaI.HindIII (CYCGRG+AAGCTT) double digest, 1865 thousand fragments (1005714+860365 sites), 1597 bp average fragment length
   5% of sequence in fragments shorter than     544 bp
  10% of sequence in fragments shorter than     847 bp
  15% of sequence in fragments shorter than    1115 bp
  20% of sequence in fragments shorter than    1370 bp
  25% of sequence in fragments shorter than    1623 bp
  30% of sequence in fragments shorter than    1879 bp
  35% of sequence in fragments shorter than    2116 bp
  40% of sequence in fragments shorter than    2386 bp
  45% of sequence in fragments shorter than    2671 bp
  50% of sequence in fragments shorter than    2971 bp
  55% of sequence in fragments shorter than    3297 bp
  60% of sequence in fragments shorter than    3659 bp
  65% of sequence in fragments shorter than    4060 bp
  70% of sequence in fragments shorter than    4515 bp
  75% of sequence in fragments shorter than    5048 bp
  80% of sequence in fragments shorter than    5694 bp
  85% of sequence in fragments shorter than    6531 bp
  90% of sequence in fragments shorter than    7730 bp
  95% of sequence in fragments shorter than    9988 bp

# PstI.ScaI (CTGCAG+AGTACT) double digest, 1864 thousand fragments (1321469+543793 sites), 1598 bp average fragment length
   5% of sequence in fragments shorter than     551 bp
  10% of sequence in fragments shorter than     837 bp
  15% of sequence in fragments shorter than    1094 bp
  20% of sequence in fragments shorter than    1335 bp
  25% of sequence in fragments shorter than    1568 bp
  30% of sequence in fragments shorter than    1810 bp
  35% of sequence in fragments shorter than    2056 bp
  40% of sequence in fragments shorter than    2306 bp
  45% of sequence in fragments shorter than    2580 bp
  50% of sequence in fragments shorter than    2865 bp
  55% of sequence in fragments shorter than    3171 bp
  60% of sequence in fragments shorter than    3512 bp
  65% of sequence in fragments shorter than    3894 bp
  70% of sequence in fragments shorter than    4329 bp
  75% of sequence in fragments shorter than    4838 bp
  80% of sequence in fragments shorter than    5437 bp
  85% of sequence in fragments shorter than    6204 bp
  90% of sequence in fragments shorter than    7295 bp
  95% of sequence in fragments shorter than    9323 bp

# BfiI.Hpy99I (ACTGGG+CGWCG) double digest, 1864 thousand fragments (1706594+157917 sites), 1598 bp average fragment length
   5% of sequence in fragments shorter than     534 bp
  10% of sequence in fragments shorter than     837 bp
  15% of sequence in fragments shorter than    1097 bp
  20% of sequence in fragments shorter than    1355 bp
  25% of sequence in fragments shorter than    1606 bp
  30% of sequence in fragments shorter than    1857 bp
  35% of sequence in fragments shorter than    2106 bp
  40% of sequence in fragments shorter than    2366 bp
  45% of sequence in fragments shorter than    2650 bp
  50% of sequence in fragments shorter than    2924 bp
  55% of sequence in fragments shorter than    3253 bp
  60% of sequence in fragments shorter than    3613 bp
  65% of sequence in fragments shorter than    4019 bp
  70% of sequence in fragments shorter than    4478 bp
  75% of sequence in fragments shorter than    5005 bp
  80% of sequence in fragments shorter than    5651 bp
  85% of sequence in fragments shorter than    6504 bp
  90% of sequence in fragments shorter than    7753 bp
  95% of sequence in fragments shorter than   10188 bp

# AflIII.SapI (ACRYGT+GCTCTTC) double digest, 1862 thousand fragments (1485394+377161 sites), 1600 bp average fragment length
   5% of sequence in fragments shorter than     573 bp
  10% of sequence in fragments shorter than     880 bp
  15% of sequence in fragments shorter than    1152 bp
  20% of sequence in fragments shorter than    1402 bp
  25% of sequence in fragments shorter than    1648 bp
  30% of sequence in fragments shorter than    1890 bp
  35% of sequence in fragments shorter than    2126 bp
  40% of sequence in fragments shorter than    2376 bp
  45% of sequence in fragments shorter than    2634 bp
  50% of sequence in fragments shorter than    2906 bp
  55% of sequence in fragments shorter than    3196 bp
  60% of sequence in fragments shorter than    3496 bp
  65% of sequence in fragments shorter than    3843 bp
  70% of sequence in fragments shorter than    4239 bp
  75% of sequence in fragments shorter than    4706 bp
  80% of sequence in fragments shorter than    5277 bp
  85% of sequence in fragments shorter than    6013 bp
  90% of sequence in fragments shorter than    7046 bp
  95% of sequence in fragments shorter than    8993 bp

# AflIII.SmaI (ACRYGT+CCCGGG) double digest, 1861 thousand fragments (1485394+376940 sites), 1600 bp average fragment length
   5% of sequence in fragments shorter than     560 bp
  10% of sequence in fragments shorter than     873 bp
  15% of sequence in fragments shorter than    1146 bp
  20% of sequence in fragments shorter than    1399 bp
  25% of sequence in fragments shorter than    1649 bp
  30% of sequence in fragments shorter than    1895 bp
  35% of sequence in fragments shorter than    2137 bp
  40% of sequence in fragments shorter than    2393 bp
  45% of sequence in fragments shorter than    2662 bp
  50% of sequence in fragments shorter than    2939 bp
  55% of sequence in fragments shorter than    3245 bp
  60% of sequence in fragments shorter than    3580 bp
  65% of sequence in fragments shorter than    3953 bp
  70% of sequence in fragments shorter than    4375 bp
  75% of sequence in fragments shorter than    4873 bp
  80% of sequence in fragments shorter than    5477 bp
  85% of sequence in fragments shorter than    6230 bp
  90% of sequence in fragments shorter than    7298 bp
  95% of sequence in fragments shorter than    9241 bp

# BscGI.HaeII (CCCGT+RGCGCY) double digest, 1860 thousand fragments (1231070+630101 sites), 1601 bp average fragment length
   5% of sequence in fragments shorter than     454 bp
  10% of sequence in fragments shorter than     835 bp
  15% of sequence in fragments shorter than    1212 bp
  20% of sequence in fragments shorter than    1589 bp
  25% of sequence in fragments shorter than    1974 bp
  30% of sequence in fragments shorter than    2375 bp
  35% of sequence in fragments shorter than    2793 bp
  40% of sequence in fragments shorter than    3245 bp
  45% of sequence in fragments shorter than    3722 bp
  50% of sequence in fragments shorter than    4242 bp
  55% of sequence in fragments shorter than    4815 bp
  60% of sequence in fragments shorter than    5451 bp
  65% of sequence in fragments shorter than    6162 bp
  70% of sequence in fragments shorter than    6984 bp
  75% of sequence in fragments shorter than    7956 bp
  80% of sequence in fragments shorter than    9161 bp
  85% of sequence in fragments shorter than   10718 bp
  90% of sequence in fragments shorter than   12893 bp
  95% of sequence in fragments shorter than   16608 bp

# AccI.NcoI (GTMKAC+CCATGG) double digest, 1860 thousand fragments (1101063+759594 sites), 1601 bp average fragment length
   5% of sequence in fragments shorter than     555 bp
  10% of sequence in fragments shorter than     854 bp
  15% of sequence in fragments shorter than    1111 bp
  20% of sequence in fragments shorter than    1350 bp
  25% of sequence in fragments shorter than    1584 bp
  30% of sequence in fragments shorter than    1818 bp
  35% of sequence in fragments shorter than    2053 bp
  40% of sequence in fragments shorter than    2298 bp
  45% of sequence in fragments shorter than    2551 bp
  50% of sequence in fragments shorter than    2816 bp
  55% of sequence in fragments shorter than    3107 bp
  60% of sequence in fragments shorter than    3421 bp
  65% of sequence in fragments shorter than    3764 bp
  70% of sequence in fragments shorter than    4158 bp
  75% of sequence in fragments shorter than    4605 bp
  80% of sequence in fragments shorter than    5149 bp
  85% of sequence in fragments shorter than    5797 bp
  90% of sequence in fragments shorter than    6758 bp
  95% of sequence in fragments shorter than    8525 bp

# BglII.PpuMI (AGATCT+RGGWCCY) double digest, 1859 thousand fragments (774732+1085138 sites), 1602 bp average fragment length
   5% of sequence in fragments shorter than     544 bp
  10% of sequence in fragments shorter than     844 bp
  15% of sequence in fragments shorter than    1110 bp
  20% of sequence in fragments shorter than    1367 bp
  25% of sequence in fragments shorter than    1601 bp
  30% of sequence in fragments shorter than    1856 bp
  35% of sequence in fragments shorter than    2113 bp
  40% of sequence in fragments shorter than    2370 bp
  45% of sequence in fragments shorter than    2651 bp
  50% of sequence in fragments shorter than    2953 bp
  55% of sequence in fragments shorter than    3280 bp
  60% of sequence in fragments shorter than    3638 bp
  65% of sequence in fragments shorter than    4035 bp
  70% of sequence in fragments shorter than    4490 bp
  75% of sequence in fragments shorter than    5025 bp
  80% of sequence in fragments shorter than    5684 bp
  85% of sequence in fragments shorter than    6552 bp
  90% of sequence in fragments shorter than    7847 bp
  95% of sequence in fragments shorter than   10476 bp

# AvaIII.StuI (ATGCAT+AGGCCT) double digest, 1858 thousand fragments (933357+925729 sites), 1603 bp average fragment length
   5% of sequence in fragments shorter than     538 bp
  10% of sequence in fragments shorter than     826 bp
  15% of sequence in fragments shorter than    1093 bp
  20% of sequence in fragments shorter than    1347 bp
  25% of sequence in fragments shorter than    1583 bp
  30% of sequence in fragments shorter than    1835 bp
  35% of sequence in fragments shorter than    2072 bp
  40% of sequence in fragments shorter than    2332 bp
  45% of sequence in fragments shorter than    2601 bp
  50% of sequence in fragments shorter than    2883 bp
  55% of sequence in fragments shorter than    3189 bp
  60% of sequence in fragments shorter than    3518 bp
  65% of sequence in fragments shorter than    3881 bp
  70% of sequence in fragments shorter than    4290 bp
  75% of sequence in fragments shorter than    4762 bp
  80% of sequence in fragments shorter than    5323 bp
  85% of sequence in fragments shorter than    6037 bp
  90% of sequence in fragments shorter than    7041 bp
  95% of sequence in fragments shorter than    8721 bp

# BglII.PvuII (AGATCT+CAGCTG) double digest, 1858 thousand fragments (774732+1084260 sites), 1603 bp average fragment length
   5% of sequence in fragments shorter than     549 bp
  10% of sequence in fragments shorter than     843 bp
  15% of sequence in fragments shorter than    1094 bp
  20% of sequence in fragments shorter than    1337 bp
  25% of sequence in fragments shorter than    1565 bp
  30% of sequence in fragments shorter than    1806 bp
  35% of sequence in fragments shorter than    2052 bp
  40% of sequence in fragments shorter than    2307 bp
  45% of sequence in fragments shorter than    2576 bp
  50% of sequence in fragments shorter than    2857 bp
  55% of sequence in fragments shorter than    3163 bp
  60% of sequence in fragments shorter than    3499 bp
  65% of sequence in fragments shorter than    3873 bp
  70% of sequence in fragments shorter than    4294 bp
  75% of sequence in fragments shorter than    4783 bp
  80% of sequence in fragments shorter than    5384 bp
  85% of sequence in fragments shorter than    6159 bp
  90% of sequence in fragments shorter than    7310 bp
  95% of sequence in fragments shorter than    9550 bp

# AvaI.XbaI (CYCGRG+TCTAGA) double digest, 1856 thousand fragments (1005714+850998 sites), 1605 bp average fragment length
   5% of sequence in fragments shorter than     556 bp
  10% of sequence in fragments shorter than     857 bp
  15% of sequence in fragments shorter than    1111 bp
  20% of sequence in fragments shorter than    1373 bp
  25% of sequence in fragments shorter than    1608 bp
  30% of sequence in fragments shorter than    1847 bp
  35% of sequence in fragments shorter than    2103 bp
  40% of sequence in fragments shorter than    2361 bp
  45% of sequence in fragments shorter than    2645 bp
  50% of sequence in fragments shorter than    2948 bp
  55% of sequence in fragments shorter than    3273 bp
  60% of sequence in fragments shorter than    3630 bp
  65% of sequence in fragments shorter than    4030 bp
  70% of sequence in fragments shorter than    4481 bp
  75% of sequence in fragments shorter than    5005 bp
  80% of sequence in fragments shorter than    5640 bp
  85% of sequence in fragments shorter than    6463 bp
  90% of sequence in fragments shorter than    7621 bp
  95% of sequence in fragments shorter than    9674 bp

# AvaI.EcoRI (CYCGRG+GAATTC) double digest, 1852 thousand fragments (1005714+847401 sites), 1608 bp average fragment length
   5% of sequence in fragments shorter than     536 bp
  10% of sequence in fragments shorter than     839 bp
  15% of sequence in fragments shorter than    1119 bp
  20% of sequence in fragments shorter than    1383 bp
  25% of sequence in fragments shorter than    1646 bp
  30% of sequence in fragments shorter than    1910 bp
  35% of sequence in fragments shorter than    2172 bp
  40% of sequence in fragments shorter than    2453 bp
  45% of sequence in fragments shorter than    2751 bp
  50% of sequence in fragments shorter than    3061 bp
  55% of sequence in fragments shorter than    3401 bp
  60% of sequence in fragments shorter than    3776 bp
  65% of sequence in fragments shorter than    4192 bp
  70% of sequence in fragments shorter than    4667 bp
  75% of sequence in fragments shorter than    5223 bp
  80% of sequence in fragments shorter than    5883 bp
  85% of sequence in fragments shorter than    6742 bp
  90% of sequence in fragments shorter than    7952 bp
  95% of sequence in fragments shorter than   10089 bp

# AflIII.BamHI (ACRYGT+GGATCC) double digest, 1850 thousand fragments (1485394+365999 sites), 1609 bp average fragment length
   5% of sequence in fragments shorter than     577 bp
  10% of sequence in fragments shorter than     881 bp
  15% of sequence in fragments shorter than    1150 bp
  20% of sequence in fragments shorter than    1404 bp
  25% of sequence in fragments shorter than    1647 bp
  30% of sequence in fragments shorter than    1893 bp
  35% of sequence in fragments shorter than    2142 bp
  40% of sequence in fragments shorter than    2399 bp
  45% of sequence in fragments shorter than    2669 bp
  50% of sequence in fragments shorter than    2945 bp
  55% of sequence in fragments shorter than    3250 bp
  60% of sequence in fragments shorter than    3582 bp
  65% of sequence in fragments shorter than    3952 bp
  70% of sequence in fragments shorter than    4370 bp
  75% of sequence in fragments shorter than    4861 bp
  80% of sequence in fragments shorter than    5432 bp
  85% of sequence in fragments shorter than    6156 bp
  90% of sequence in fragments shorter than    7190 bp
  95% of sequence in fragments shorter than    9101 bp

# HgiCI.SexAI (GGYRCC+ACCWGGT) double digest, 1848 thousand fragments (1550877+297900 sites), 1612 bp average fragment length
   5% of sequence in fragments shorter than     535 bp
  10% of sequence in fragments shorter than     837 bp
  15% of sequence in fragments shorter than    1113 bp
  20% of sequence in fragments shorter than    1352 bp
  25% of sequence in fragments shorter than    1603 bp
  30% of sequence in fragments shorter than    1872 bp
  35% of sequence in fragments shorter than    2151 bp
  40% of sequence in fragments shorter than    2447 bp
  45% of sequence in fragments shorter than    2756 bp
  50% of sequence in fragments shorter than    3073 bp
  55% of sequence in fragments shorter than    3435 bp
  60% of sequence in fragments shorter than    3836 bp
  65% of sequence in fragments shorter than    4286 bp
  70% of sequence in fragments shorter than    4806 bp
  75% of sequence in fragments shorter than    5425 bp
  80% of sequence in fragments shorter than    6190 bp
  85% of sequence in fragments shorter than    7212 bp
  90% of sequence in fragments shorter than    8739 bp
  95% of sequence in fragments shorter than   11897 bp

# BsmI.SexAI (GAATGC+ACCWGGT) double digest, 1846 thousand fragments (1549350+297900 sites), 1613 bp average fragment length
   5% of sequence in fragments shorter than     546 bp
  10% of sequence in fragments shorter than     939 bp
  15% of sequence in fragments shorter than    1249 bp
  20% of sequence in fragments shorter than    1546 bp
  25% of sequence in fragments shorter than    1829 bp
  30% of sequence in fragments shorter than    2108 bp
  35% of sequence in fragments shorter than    2390 bp
  40% of sequence in fragments shorter than    2673 bp
  45% of sequence in fragments shorter than    2965 bp
  50% of sequence in fragments shorter than    3277 bp
  55% of sequence in fragments shorter than    3615 bp
  60% of sequence in fragments shorter than    3982 bp
  65% of sequence in fragments shorter than    4378 bp
  70% of sequence in fragments shorter than    4828 bp
  75% of sequence in fragments shorter than    5352 bp
  80% of sequence in fragments shorter than    5976 bp
  85% of sequence in fragments shorter than    6762 bp
  90% of sequence in fragments shorter than    7843 bp
  95% of sequence in fragments shorter than    9675 bp

# NcoI.PpuMI (CCATGG+RGGWCCY) double digest, 1844 thousand fragments (759594+1085138 sites), 1615 bp average fragment length
   5% of sequence in fragments shorter than     529 bp
  10% of sequence in fragments shorter than     850 bp
  15% of sequence in fragments shorter than    1138 bp
  20% of sequence in fragments shorter than    1387 bp
  25% of sequence in fragments shorter than    1665 bp
  30% of sequence in fragments shorter than    1949 bp
  35% of sequence in fragments shorter than    2246 bp
  40% of sequence in fragments shorter than    2558 bp
  45% of sequence in fragments shorter than    2890 bp
  50% of sequence in fragments shorter than    3232 bp
  55% of sequence in fragments shorter than    3589 bp
  60% of sequence in fragments shorter than    4000 bp
  65% of sequence in fragments shorter than    4469 bp
  70% of sequence in fragments shorter than    5001 bp
  75% of sequence in fragments shorter than    5644 bp
  80% of sequence in fragments shorter than    6439 bp
  85% of sequence in fragments shorter than    7496 bp
  90% of sequence in fragments shorter than    9031 bp
  95% of sequence in fragments shorter than   12104 bp

# NcoI.PvuII (CCATGG+CAGCTG) double digest, 1843 thousand fragments (759594+1084260 sites), 1616 bp average fragment length
   5% of sequence in fragments shorter than     539 bp
  10% of sequence in fragments shorter than     850 bp
  15% of sequence in fragments shorter than    1122 bp
  20% of sequence in fragments shorter than    1382 bp
  25% of sequence in fragments shorter than    1638 bp
  30% of sequence in fragments shorter than    1902 bp
  35% of sequence in fragments shorter than    2177 bp
  40% of sequence in fragments shorter than    2451 bp
  45% of sequence in fragments shorter than    2750 bp
  50% of sequence in fragments shorter than    3062 bp
  55% of sequence in fragments shorter than    3411 bp
  60% of sequence in fragments shorter than    3798 bp
  65% of sequence in fragments shorter than    4218 bp
  70% of sequence in fragments shorter than    4700 bp
  75% of sequence in fragments shorter than    5231 bp
  80% of sequence in fragments shorter than    5922 bp
  85% of sequence in fragments shorter than    6829 bp
  90% of sequence in fragments shorter than    8152 bp
  95% of sequence in fragments shorter than   10693 bp

# ClaI.HhaI (ATCGAT+GCGC) double digest, 1842 thousand fragments (86477+1756502 sites), 1617 bp average fragment length
   5% of sequence in fragments shorter than     544 bp
  10% of sequence in fragments shorter than     950 bp
  15% of sequence in fragments shorter than    1356 bp
  20% of sequence in fragments shorter than    1760 bp
  25% of sequence in fragments shorter than    2172 bp
  30% of sequence in fragments shorter than    2611 bp
  35% of sequence in fragments shorter than    3073 bp
  40% of sequence in fragments shorter than    3563 bp
  45% of sequence in fragments shorter than    4100 bp
  50% of sequence in fragments shorter than    4681 bp
  55% of sequence in fragments shorter than    5321 bp
  60% of sequence in fragments shorter than    6035 bp
  65% of sequence in fragments shorter than    6850 bp
  70% of sequence in fragments shorter than    7784 bp
  75% of sequence in fragments shorter than    8887 bp
  80% of sequence in fragments shorter than   10274 bp
  85% of sequence in fragments shorter than   12036 bp
  90% of sequence in fragments shorter than   14519 bp
  95% of sequence in fragments shorter than   18921 bp

# FauI.SpeI (CCCGC+ACTAGT) double digest, 1839 thousand fragments (1439772+400286 sites), 1619 bp average fragment length
   5% of sequence in fragments shorter than     570 bp
  10% of sequence in fragments shorter than     941 bp
  15% of sequence in fragments shorter than    1295 bp
  20% of sequence in fragments shorter than    1634 bp
  25% of sequence in fragments shorter than    1973 bp
  30% of sequence in fragments shorter than    2316 bp
  35% of sequence in fragments shorter than    2673 bp
  40% of sequence in fragments shorter than    3049 bp
  45% of sequence in fragments shorter than    3448 bp
  50% of sequence in fragments shorter than    3876 bp
  55% of sequence in fragments shorter than    4316 bp
  60% of sequence in fragments shorter than    4829 bp
  65% of sequence in fragments shorter than    5373 bp
  70% of sequence in fragments shorter than    6022 bp
  75% of sequence in fragments shorter than    6785 bp
  80% of sequence in fragments shorter than    7708 bp
  85% of sequence in fragments shorter than    8907 bp
  90% of sequence in fragments shorter than   10671 bp
  95% of sequence in fragments shorter than   13916 bp

# AccI.BclI (GTMKAC+TGATCA) double digest, 1839 thousand fragments (1101063+738785 sites), 1620 bp average fragment length
   5% of sequence in fragments shorter than     572 bp
  10% of sequence in fragments shorter than     859 bp
  15% of sequence in fragments shorter than    1107 bp
  20% of sequence in fragments shorter than    1341 bp
  25% of sequence in fragments shorter than    1567 bp
  30% of sequence in fragments shorter than    1796 bp
  35% of sequence in fragments shorter than    2028 bp
  40% of sequence in fragments shorter than    2270 bp
  45% of sequence in fragments shorter than    2503 bp
  50% of sequence in fragments shorter than    2762 bp
  55% of sequence in fragments shorter than    3049 bp
  60% of sequence in fragments shorter than    3362 bp
  65% of sequence in fragments shorter than    3700 bp
  70% of sequence in fragments shorter than    4089 bp
  75% of sequence in fragments shorter than    4551 bp
  80% of sequence in fragments shorter than    5109 bp
  85% of sequence in fragments shorter than    5835 bp
  90% of sequence in fragments shorter than    6874 bp
  95% of sequence in fragments shorter than    8823 bp

# BfiI.SnaBI (ACTGGG+TACGTA) double digest, 1838 thousand fragments (1706594+132299 sites), 1620 bp average fragment length
   5% of sequence in fragments shorter than     553 bp
  10% of sequence in fragments shorter than     855 bp
  15% of sequence in fragments shorter than    1111 bp
  20% of sequence in fragments shorter than    1363 bp
  25% of sequence in fragments shorter than    1601 bp
  30% of sequence in fragments shorter than    1845 bp
  35% of sequence in fragments shorter than    2087 bp
  40% of sequence in fragments shorter than    2333 bp
  45% of sequence in fragments shorter than    2606 bp
  50% of sequence in fragments shorter than    2864 bp
  55% of sequence in fragments shorter than    3178 bp
  60% of sequence in fragments shorter than    3513 bp
  65% of sequence in fragments shorter than    3890 bp
  70% of sequence in fragments shorter than    4319 bp
  75% of sequence in fragments shorter than    4819 bp
  80% of sequence in fragments shorter than    5409 bp
  85% of sequence in fragments shorter than    6183 bp
  90% of sequence in fragments shorter than    7318 bp
  95% of sequence in fragments shorter than    9488 bp

# BsrBI.EsaBC3I (CCGCTC+TCGA) double digest, 1838 thousand fragments (235523+1603346 sites), 1621 bp average fragment length
   5% of sequence in fragments shorter than     543 bp
  10% of sequence in fragments shorter than     845 bp
  15% of sequence in fragments shorter than    1112 bp
  20% of sequence in fragments shorter than    1372 bp
  25% of sequence in fragments shorter than    1621 bp
  30% of sequence in fragments shorter than    1884 bp
  35% of sequence in fragments shorter than    2155 bp
  40% of sequence in fragments shorter than    2436 bp
  45% of sequence in fragments shorter than    2734 bp
  50% of sequence in fragments shorter than    3050 bp
  55% of sequence in fragments shorter than    3393 bp
  60% of sequence in fragments shorter than    3770 bp
  65% of sequence in fragments shorter than    4190 bp
  70% of sequence in fragments shorter than    4673 bp
  75% of sequence in fragments shorter than    5243 bp
  80% of sequence in fragments shorter than    5925 bp
  85% of sequence in fragments shorter than    6815 bp
  90% of sequence in fragments shorter than    8033 bp
  95% of sequence in fragments shorter than   10154 bp

# BspHI.HindIII (TCATGA+AAGCTT) double digest, 1838 thousand fragments (978290+860365 sites), 1621 bp average fragment length
   5% of sequence in fragments shorter than     567 bp
  10% of sequence in fragments shorter than     853 bp
  15% of sequence in fragments shorter than    1112 bp
  20% of sequence in fragments shorter than    1351 bp
  25% of sequence in fragments shorter than    1587 bp
  30% of sequence in fragments shorter than    1822 bp
  35% of sequence in fragments shorter than    2033 bp
  40% of sequence in fragments shorter than    2270 bp
  45% of sequence in fragments shorter than    2523 bp
  50% of sequence in fragments shorter than    2789 bp
  55% of sequence in fragments shorter than    3077 bp
  60% of sequence in fragments shorter than    3393 bp
  65% of sequence in fragments shorter than    3749 bp
  70% of sequence in fragments shorter than    4150 bp
  75% of sequence in fragments shorter than    4620 bp
  80% of sequence in fragments shorter than    5199 bp
  85% of sequence in fragments shorter than    5967 bp
  90% of sequence in fragments shorter than    7075 bp
  95% of sequence in fragments shorter than    9235 bp

# BspMI.SpeI (ACCTGC+ACTAGT) double digest, 1837 thousand fragments (1438129+400286 sites), 1621 bp average fragment length
   5% of sequence in fragments shorter than     554 bp
  10% of sequence in fragments shorter than     847 bp
  15% of sequence in fragments shorter than    1106 bp
  20% of sequence in fragments shorter than    1360 bp
  25% of sequence in fragments shorter than    1600 bp
  30% of sequence in fragments shorter than    1848 bp
  35% of sequence in fragments shorter than    2079 bp
  40% of sequence in fragments shorter than    2337 bp
  45% of sequence in fragments shorter than    2608 bp
  50% of sequence in fragments shorter than    2860 bp
  55% of sequence in fragments shorter than    3165 bp
  60% of sequence in fragments shorter than    3490 bp
  65% of sequence in fragments shorter than    3858 bp
  70% of sequence in fragments shorter than    4277 bp
  75% of sequence in fragments shorter than    4755 bp
  80% of sequence in fragments shorter than    5331 bp
  85% of sequence in fragments shorter than    6080 bp
  90% of sequence in fragments shorter than    7126 bp
  95% of sequence in fragments shorter than    8953 bp

# HgiCI.NheI (GGYRCC+GCTAGC) double digest, 1837 thousand fragments (1550877+287384 sites), 1621 bp average fragment length
   5% of sequence in fragments shorter than     534 bp
  10% of sequence in fragments shorter than     845 bp
  15% of sequence in fragments shorter than    1117 bp
  20% of sequence in fragments shorter than    1354 bp
  25% of sequence in fragments shorter than    1610 bp
  30% of sequence in fragments shorter than    1877 bp
  35% of sequence in fragments shorter than    2148 bp
  40% of sequence in fragments shorter than    2432 bp
  45% of sequence in fragments shorter than    2734 bp
  50% of sequence in fragments shorter than    3058 bp
  55% of sequence in fragments shorter than    3412 bp
  60% of sequence in fragments shorter than    3807 bp
  65% of sequence in fragments shorter than    4253 bp
  70% of sequence in fragments shorter than    4764 bp
  75% of sequence in fragments shorter than    5378 bp
  80% of sequence in fragments shorter than    6125 bp
  85% of sequence in fragments shorter than    7117 bp
  90% of sequence in fragments shorter than    8585 bp
  95% of sequence in fragments shorter than   11520 bp

# BsmI.KpnI (GAATGC+GGTACC) double digest, 1836 thousand fragments (1549350+287974 sites), 1622 bp average fragment length
   5% of sequence in fragments shorter than     522 bp
  10% of sequence in fragments shorter than     922 bp
  15% of sequence in fragments shorter than    1248 bp
  20% of sequence in fragments shorter than    1557 bp
  25% of sequence in fragments shorter than    1840 bp
  30% of sequence in fragments shorter than    2121 bp
  35% of sequence in fragments shorter than    2411 bp
  40% of sequence in fragments shorter than    2707 bp
  45% of sequence in fragments shorter than    3016 bp
  50% of sequence in fragments shorter than    3344 bp
  55% of sequence in fragments shorter than    3690 bp
  60% of sequence in fragments shorter than    4067 bp
  65% of sequence in fragments shorter than    4482 bp
  70% of sequence in fragments shorter than    4950 bp
  75% of sequence in fragments shorter than    5490 bp
  80% of sequence in fragments shorter than    6134 bp
  85% of sequence in fragments shorter than    6947 bp
  90% of sequence in fragments shorter than    8065 bp
  95% of sequence in fragments shorter than    9954 bp

# AvaIII.NdeI (ATGCAT+CATATG) double digest, 1836 thousand fragments (933357+903360 sites), 1622 bp average fragment length
   5% of sequence in fragments shorter than     570 bp
  10% of sequence in fragments shorter than     872 bp
  15% of sequence in fragments shorter than    1142 bp
  20% of sequence in fragments shorter than    1388 bp
  25% of sequence in fragments shorter than    1642 bp
  30% of sequence in fragments shorter than    1900 bp
  35% of sequence in fragments shorter than    2160 bp
  40% of sequence in fragments shorter than    2434 bp
  45% of sequence in fragments shorter than    2725 bp
  50% of sequence in fragments shorter than    3034 bp
  55% of sequence in fragments shorter than    3370 bp
  60% of sequence in fragments shorter than    3749 bp
  65% of sequence in fragments shorter than    4176 bp
  70% of sequence in fragments shorter than    4664 bp
  75% of sequence in fragments shorter than    5234 bp
  80% of sequence in fragments shorter than    5944 bp
  85% of sequence in fragments shorter than    6898 bp
  90% of sequence in fragments shorter than    8378 bp
  95% of sequence in fragments shorter than   11532 bp

# BsmI.NheI (GAATGC+GCTAGC) double digest, 1836 thousand fragments (1549350+287384 sites), 1622 bp average fragment length
   5% of sequence in fragments shorter than     562 bp
  10% of sequence in fragments shorter than     925 bp
  15% of sequence in fragments shorter than    1247 bp
  20% of sequence in fragments shorter than    1554 bp
  25% of sequence in fragments shorter than    1840 bp
  30% of sequence in fragments shorter than    2128 bp
  35% of sequence in fragments shorter than    2422 bp
  40% of sequence in fragments shorter than    2722 bp
  45% of sequence in fragments shorter than    3033 bp
  50% of sequence in fragments shorter than    3362 bp
  55% of sequence in fragments shorter than    3716 bp
  60% of sequence in fragments shorter than    4099 bp
  65% of sequence in fragments shorter than    4520 bp
  70% of sequence in fragments shorter than    4999 bp
  75% of sequence in fragments shorter than    5554 bp
  80% of sequence in fragments shorter than    6208 bp
  85% of sequence in fragments shorter than    7028 bp
  90% of sequence in fragments shorter than    8174 bp
  95% of sequence in fragments shorter than   10094 bp

# AccI.FnuDII (GTMKAC+CGCG) double digest, 1834 thousand fragments (1101063+733940 sites), 1624 bp average fragment length
   5% of sequence in fragments shorter than     607 bp
  10% of sequence in fragments shorter than     971 bp
  15% of sequence in fragments shorter than    1280 bp
  20% of sequence in fragments shorter than    1566 bp
  25% of sequence in fragments shorter than    1850 bp
  30% of sequence in fragments shorter than    2130 bp
  35% of sequence in fragments shorter than    2416 bp
  40% of sequence in fragments shorter than    2710 bp
  45% of sequence in fragments shorter than    3014 bp
  50% of sequence in fragments shorter than    3342 bp
  55% of sequence in fragments shorter than    3691 bp
  60% of sequence in fragments shorter than    4078 bp
  65% of sequence in fragments shorter than    4502 bp
  70% of sequence in fragments shorter than    4980 bp
  75% of sequence in fragments shorter than    5522 bp
  80% of sequence in fragments shorter than    6118 bp
  85% of sequence in fragments shorter than    6954 bp
  90% of sequence in fragments shorter than    8154 bp
  95% of sequence in fragments shorter than   10317 bp

# BscGI.SacI (CCCGT+GAGCTC) double digest, 1833 thousand fragments (1231070+603323 sites), 1624 bp average fragment length
   5% of sequence in fragments shorter than     480 bp
  10% of sequence in fragments shorter than     799 bp
  15% of sequence in fragments shorter than    1102 bp
  20% of sequence in fragments shorter than    1394 bp
  25% of sequence in fragments shorter than    1693 bp
  30% of sequence in fragments shorter than    1999 bp
  35% of sequence in fragments shorter than    2320 bp
  40% of sequence in fragments shorter than    2658 bp
  45% of sequence in fragments shorter than    3020 bp
  50% of sequence in fragments shorter than    3408 bp
  55% of sequence in fragments shorter than    3832 bp
  60% of sequence in fragments shorter than    4298 bp
  65% of sequence in fragments shorter than    4812 bp
  70% of sequence in fragments shorter than    5409 bp
  75% of sequence in fragments shorter than    6105 bp
  80% of sequence in fragments shorter than    6953 bp
  85% of sequence in fragments shorter than    8055 bp
  90% of sequence in fragments shorter than    9605 bp
  95% of sequence in fragments shorter than   12276 bp

# EsaBC3I.KasI (TCGA+GGCGCC) double digest, 1833 thousand fragments (1603346+230381 sites), 1625 bp average fragment length
   5% of sequence in fragments shorter than     549 bp
  10% of sequence in fragments shorter than     862 bp
  15% of sequence in fragments shorter than    1141 bp
  20% of sequence in fragments shorter than    1395 bp
  25% of sequence in fragments shorter than    1663 bp
  30% of sequence in fragments shorter than    1930 bp
  35% of sequence in fragments shorter than    2211 bp
  40% of sequence in fragments shorter than    2502 bp
  45% of sequence in fragments shorter than    2808 bp
  50% of sequence in fragments shorter than    3128 bp
  55% of sequence in fragments shorter than    3482 bp
  60% of sequence in fragments shorter than    3870 bp
  65% of sequence in fragments shorter than    4303 bp
  70% of sequence in fragments shorter than    4803 bp
  75% of sequence in fragments shorter than    5386 bp
  80% of sequence in fragments shorter than    6093 bp
  85% of sequence in fragments shorter than    7008 bp
  90% of sequence in fragments shorter than    8261 bp
  95% of sequence in fragments shorter than   10447 bp

# BfiI.NaeI (ACTGGG+GCCGGC) double digest, 1831 thousand fragments (1706594+125692 sites), 1626 bp average fragment length
   5% of sequence in fragments shorter than     544 bp
  10% of sequence in fragments shorter than     851 bp
  15% of sequence in fragments shorter than    1116 bp
  20% of sequence in fragments shorter than    1377 bp
  25% of sequence in fragments shorter than    1633 bp
  30% of sequence in fragments shorter than    1887 bp
  35% of sequence in fragments shorter than    2142 bp
  40% of sequence in fragments shorter than    2406 bp
  45% of sequence in fragments shorter than    2696 bp
  50% of sequence in fragments shorter than    2975 bp
  55% of sequence in fragments shorter than    3309 bp
  60% of sequence in fragments shorter than    3677 bp
  65% of sequence in fragments shorter than    4093 bp
  70% of sequence in fragments shorter than    4558 bp
  75% of sequence in fragments shorter than    5096 bp
  80% of sequence in fragments shorter than    5757 bp
  85% of sequence in fragments shorter than    6628 bp
  90% of sequence in fragments shorter than    7918 bp
  95% of sequence in fragments shorter than   10448 bp

# EsaBC3I.SwaI (TCGA+ATTTAAAT) double digest, 1829 thousand fragments (1603346+226602 sites), 1628 bp average fragment length
   5% of sequence in fragments shorter than     559 bp
  10% of sequence in fragments shorter than     858 bp
  15% of sequence in fragments shorter than    1115 bp
  20% of sequence in fragments shorter than    1362 bp
  25% of sequence in fragments shorter than    1594 bp
  30% of sequence in fragments shorter than    1836 bp
  35% of sequence in fragments shorter than    2087 bp
  40% of sequence in fragments shorter than    2347 bp
  45% of sequence in fragments shorter than    2619 bp
  50% of sequence in fragments shorter than    2907 bp
  55% of sequence in fragments shorter than    3209 bp
  60% of sequence in fragments shorter than    3546 bp
  65% of sequence in fragments shorter than    3920 bp
  70% of sequence in fragments shorter than    4342 bp
  75% of sequence in fragments shorter than    4839 bp
  80% of sequence in fragments shorter than    5434 bp
  85% of sequence in fragments shorter than    6198 bp
  90% of sequence in fragments shorter than    7273 bp
  95% of sequence in fragments shorter than    9090 bp

# BspHI.XbaI (TCATGA+TCTAGA) double digest, 1828 thousand fragments (978290+850998 sites), 1629 bp average fragment length
   5% of sequence in fragments shorter than     570 bp
  10% of sequence in fragments shorter than     858 bp
  15% of sequence in fragments shorter than    1094 bp
  20% of sequence in fragments shorter than    1328 bp
  25% of sequence in fragments shorter than    1562 bp
  30% of sequence in fragments shorter than    1797 bp
  35% of sequence in fragments shorter than    2035 bp
  40% of sequence in fragments shorter than    2277 bp
  45% of sequence in fragments shorter than    2539 bp
  50% of sequence in fragments shorter than    2820 bp
  55% of sequence in fragments shorter than    3117 bp
  60% of sequence in fragments shorter than    3448 bp
  65% of sequence in fragments shorter than    3811 bp
  70% of sequence in fragments shorter than    4219 bp
  75% of sequence in fragments shorter than    4697 bp
  80% of sequence in fragments shorter than    5279 bp
  85% of sequence in fragments shorter than    6031 bp
  90% of sequence in fragments shorter than    7123 bp
  95% of sequence in fragments shorter than    9101 bp

# FauI.HpaI (CCCGC+GTTAAC) double digest, 1828 thousand fragments (1439772+389418 sites), 1629 bp average fragment length
   5% of sequence in fragments shorter than     572 bp
  10% of sequence in fragments shorter than     963 bp
  15% of sequence in fragments shorter than    1320 bp
  20% of sequence in fragments shorter than    1656 bp
  25% of sequence in fragments shorter than    2000 bp
  30% of sequence in fragments shorter than    2333 bp
  35% of sequence in fragments shorter than    2697 bp
  40% of sequence in fragments shorter than    3083 bp
  45% of sequence in fragments shorter than    3488 bp
  50% of sequence in fragments shorter than    3929 bp
  55% of sequence in fragments shorter than    4388 bp
  60% of sequence in fragments shorter than    4919 bp
  65% of sequence in fragments shorter than    5513 bp
  70% of sequence in fragments shorter than    6196 bp
  75% of sequence in fragments shorter than    7003 bp
  80% of sequence in fragments shorter than    7995 bp
  85% of sequence in fragments shorter than    9276 bp
  90% of sequence in fragments shorter than   11141 bp
  95% of sequence in fragments shorter than   14652 bp

# NdeI.StuI (CATATG+AGGCCT) double digest, 1828 thousand fragments (903360+925729 sites), 1629 bp average fragment length
   5% of sequence in fragments shorter than     544 bp
  10% of sequence in fragments shorter than     827 bp
  15% of sequence in fragments shorter than    1102 bp
  20% of sequence in fragments shorter than    1350 bp
  25% of sequence in fragments shorter than    1590 bp
  30% of sequence in fragments shorter than    1849 bp
  35% of sequence in fragments shorter than    2089 bp
  40% of sequence in fragments shorter than    2357 bp
  45% of sequence in fragments shorter than    2636 bp
  50% of sequence in fragments shorter than    2918 bp
  55% of sequence in fragments shorter than    3229 bp
  60% of sequence in fragments shorter than    3565 bp
  65% of sequence in fragments shorter than    3939 bp
  70% of sequence in fragments shorter than    4355 bp
  75% of sequence in fragments shorter than    4835 bp
  80% of sequence in fragments shorter than    5405 bp
  85% of sequence in fragments shorter than    6129 bp
  90% of sequence in fragments shorter than    7139 bp
  95% of sequence in fragments shorter than    8864 bp

# BfiI.XhoI (ACTGGG+CTCGAG) double digest, 1827 thousand fragments (1706594+121308 sites), 1630 bp average fragment length
   5% of sequence in fragments shorter than     549 bp
  10% of sequence in fragments shorter than     852 bp
  15% of sequence in fragments shorter than    1109 bp
  20% of sequence in fragments shorter than    1358 bp
  25% of sequence in fragments shorter than    1607 bp
  30% of sequence in fragments shorter than    1856 bp
  35% of sequence in fragments shorter than    2111 bp
  40% of sequence in fragments shorter than    2377 bp
  45% of sequence in fragments shorter than    2660 bp
  50% of sequence in fragments shorter than    2932 bp
  55% of sequence in fragments shorter than    3257 bp
  60% of sequence in fragments shorter than    3614 bp
  65% of sequence in fragments shorter than    4015 bp
  70% of sequence in fragments shorter than    4464 bp
  75% of sequence in fragments shorter than    4992 bp
  80% of sequence in fragments shorter than    5635 bp
  85% of sequence in fragments shorter than    6480 bp
  90% of sequence in fragments shorter than    7722 bp
  95% of sequence in fragments shorter than   10161 bp

# AatII.HhaI (GACGTC+GCGC) double digest, 1827 thousand fragments (71089+1756502 sites), 1630 bp average fragment length
   5% of sequence in fragments shorter than     543 bp
  10% of sequence in fragments shorter than     957 bp
  15% of sequence in fragments shorter than    1373 bp
  20% of sequence in fragments shorter than    1780 bp
  25% of sequence in fragments shorter than    2222 bp
  30% of sequence in fragments shorter than    2685 bp
  35% of sequence in fragments shorter than    3181 bp
  40% of sequence in fragments shorter than    3712 bp
  45% of sequence in fragments shorter than    4281 bp
  50% of sequence in fragments shorter than    4919 bp
  55% of sequence in fragments shorter than    5617 bp
  60% of sequence in fragments shorter than    6390 bp
  65% of sequence in fragments shorter than    7281 bp
  70% of sequence in fragments shorter than    8311 bp
  75% of sequence in fragments shorter than    9546 bp
  80% of sequence in fragments shorter than   11054 bp
  85% of sequence in fragments shorter than   13035 bp
  90% of sequence in fragments shorter than   15803 bp
  95% of sequence in fragments shorter than   20681 bp

# BspMI.HpaI (ACCTGC+GTTAAC) double digest, 1827 thousand fragments (1438129+389418 sites), 1631 bp average fragment length
   5% of sequence in fragments shorter than     557 bp
  10% of sequence in fragments shorter than     861 bp
  15% of sequence in fragments shorter than    1119 bp
  20% of sequence in fragments shorter than    1372 bp
  25% of sequence in fragments shorter than    1611 bp
  30% of sequence in fragments shorter than    1854 bp
  35% of sequence in fragments shorter than    2093 bp
  40% of sequence in fragments shorter than    2353 bp
  45% of sequence in fragments shorter than    2628 bp
  50% of sequence in fragments shorter than    2914 bp
  55% of sequence in fragments shorter than    3223 bp
  60% of sequence in fragments shorter than    3516 bp
  65% of sequence in fragments shorter than    3883 bp
  70% of sequence in fragments shorter than    4304 bp
  75% of sequence in fragments shorter than    4794 bp
  80% of sequence in fragments shorter than    5383 bp
  85% of sequence in fragments shorter than    6136 bp
  90% of sequence in fragments shorter than    7185 bp
  95% of sequence in fragments shorter than    9019 bp

# AvrII.BscGI (CCTAGG+CCCGT) double digest, 1825 thousand fragments (594956+1231070 sites), 1632 bp average fragment length
   5% of sequence in fragments shorter than     494 bp
  10% of sequence in fragments shorter than     800 bp
  15% of sequence in fragments shorter than    1101 bp
  20% of sequence in fragments shorter than    1394 bp
  25% of sequence in fragments shorter than    1689 bp
  30% of sequence in fragments shorter than    1987 bp
  35% of sequence in fragments shorter than    2293 bp
  40% of sequence in fragments shorter than    2612 bp
  45% of sequence in fragments shorter than    2942 bp
  50% of sequence in fragments shorter than    3299 bp
  55% of sequence in fragments shorter than    3689 bp
  60% of sequence in fragments shorter than    4113 bp
  65% of sequence in fragments shorter than    4583 bp
  70% of sequence in fragments shorter than    5137 bp
  75% of sequence in fragments shorter than    5784 bp
  80% of sequence in fragments shorter than    6566 bp
  85% of sequence in fragments shorter than    7575 bp
  90% of sequence in fragments shorter than    9007 bp
  95% of sequence in fragments shorter than   11467 bp

# BspHI.EcoRI (TCATGA+GAATTC) double digest, 1825 thousand fragments (978290+847401 sites), 1632 bp average fragment length
   5% of sequence in fragments shorter than     569 bp
  10% of sequence in fragments shorter than     847 bp
  15% of sequence in fragments shorter than    1115 bp
  20% of sequence in fragments shorter than    1368 bp
  25% of sequence in fragments shorter than    1604 bp
  30% of sequence in fragments shorter than    1847 bp
  35% of sequence in fragments shorter than    2085 bp
  40% of sequence in fragments shorter than    2331 bp
  45% of sequence in fragments shorter than    2592 bp
  50% of sequence in fragments shorter than    2868 bp
  55% of sequence in fragments shorter than    3168 bp
  60% of sequence in fragments shorter than    3488 bp
  65% of sequence in fragments shorter than    3849 bp
  70% of sequence in fragments shorter than    4253 bp
  75% of sequence in fragments shorter than    4727 bp
  80% of sequence in fragments shorter than    5304 bp
  85% of sequence in fragments shorter than    6047 bp
  90% of sequence in fragments shorter than    7122 bp
  95% of sequence in fragments shorter than    9064 bp

# BclI.PpuMI (TGATCA+RGGWCCY) double digest, 1823 thousand fragments (738785+1085138 sites), 1634 bp average fragment length
   5% of sequence in fragments shorter than     560 bp
  10% of sequence in fragments shorter than     858 bp
  15% of sequence in fragments shorter than    1119 bp
  20% of sequence in fragments shorter than    1374 bp
  25% of sequence in fragments shorter than    1627 bp
  30% of sequence in fragments shorter than    1878 bp
  35% of sequence in fragments shorter than    2132 bp
  40% of sequence in fragments shorter than    2399 bp
  45% of sequence in fragments shorter than    2671 bp
  50% of sequence in fragments shorter than    2959 bp
  55% of sequence in fragments shorter than    3274 bp
  60% of sequence in fragments shorter than    3626 bp
  65% of sequence in fragments shorter than    4016 bp
  70% of sequence in fragments shorter than    4467 bp
  75% of sequence in fragments shorter than    4998 bp
  80% of sequence in fragments shorter than    5645 bp
  85% of sequence in fragments shorter than    6501 bp
  90% of sequence in fragments shorter than    7736 bp
  95% of sequence in fragments shorter than   10276 bp

# HhaI.SacII (GCGC+CCGCGG) double digest, 1822 thousand fragments (1756502+66901 sites), 1634 bp average fragment length
   5% of sequence in fragments shorter than     555 bp
  10% of sequence in fragments shorter than     986 bp
  15% of sequence in fragments shorter than    1416 bp
  20% of sequence in fragments shorter than    1852 bp
  25% of sequence in fragments shorter than    2319 bp
  30% of sequence in fragments shorter than    2806 bp
  35% of sequence in fragments shorter than    3333 bp
  40% of sequence in fragments shorter than    3895 bp
  45% of sequence in fragments shorter than    4506 bp
  50% of sequence in fragments shorter than    5182 bp
  55% of sequence in fragments shorter than    5924 bp
  60% of sequence in fragments shorter than    6756 bp
  65% of sequence in fragments shorter than    7704 bp
  70% of sequence in fragments shorter than    8806 bp
  75% of sequence in fragments shorter than   10134 bp
  80% of sequence in fragments shorter than   11763 bp
  85% of sequence in fragments shorter than   13863 bp
  90% of sequence in fragments shorter than   16890 bp
  95% of sequence in fragments shorter than   22162 bp

# BclI.PvuII (TGATCA+CAGCTG) double digest, 1822 thousand fragments (738785+1084260 sites), 1634 bp average fragment length
   5% of sequence in fragments shorter than     568 bp
  10% of sequence in fragments shorter than     867 bp
  15% of sequence in fragments shorter than    1127 bp
  20% of sequence in fragments shorter than    1375 bp
  25% of sequence in fragments shorter than    1615 bp
  30% of sequence in fragments shorter than    1854 bp
  35% of sequence in fragments shorter than    2097 bp
  40% of sequence in fragments shorter than    2353 bp
  45% of sequence in fragments shorter than    2618 bp
  50% of sequence in fragments shorter than    2891 bp
  55% of sequence in fragments shorter than    3179 bp
  60% of sequence in fragments shorter than    3511 bp
  65% of sequence in fragments shorter than    3880 bp
  70% of sequence in fragments shorter than    4297 bp
  75% of sequence in fragments shorter than    4788 bp
  80% of sequence in fragments shorter than    5393 bp
  85% of sequence in fragments shorter than    6178 bp
  90% of sequence in fragments shorter than    7307 bp
  95% of sequence in fragments shorter than    9545 bp

# AflIII.BstEII (ACRYGT+GGTNACC) double digest, 1820 thousand fragments (1485394+335865 sites), 1636 bp average fragment length
   5% of sequence in fragments shorter than     586 bp
  10% of sequence in fragments shorter than     907 bp
  15% of sequence in fragments shorter than    1185 bp
  20% of sequence in fragments shorter than    1443 bp
  25% of sequence in fragments shorter than    1695 bp
  30% of sequence in fragments shorter than    1942 bp
  35% of sequence in fragments shorter than    2188 bp
  40% of sequence in fragments shorter than    2446 bp
  45% of sequence in fragments shorter than    2715 bp
  50% of sequence in fragments shorter than    2992 bp
  55% of sequence in fragments shorter than    3293 bp
  60% of sequence in fragments shorter than    3620 bp
  65% of sequence in fragments shorter than    3979 bp
  70% of sequence in fragments shorter than    4351 bp
  75% of sequence in fragments shorter than    4813 bp
  80% of sequence in fragments shorter than    5388 bp
  85% of sequence in fragments shorter than    6137 bp
  90% of sequence in fragments shorter than    7195 bp
  95% of sequence in fragments shorter than    9198 bp

# AclI.PsiI (AACGTT+TTATAA) double digest, 1820 thousand fragments (172706+1647912 sites), 1637 bp average fragment length
   5% of sequence in fragments shorter than     551 bp
  10% of sequence in fragments shorter than     857 bp
  15% of sequence in fragments shorter than    1122 bp
  20% of sequence in fragments shorter than    1378 bp
  25% of sequence in fragments shorter than    1636 bp
  30% of sequence in fragments shorter than    1898 bp
  35% of sequence in fragments shorter than    2171 bp
  40% of sequence in fragments shorter than    2454 bp
  45% of sequence in fragments shorter than    2767 bp
  50% of sequence in fragments shorter than    3092 bp
  55% of sequence in fragments shorter than    3461 bp
  60% of sequence in fragments shorter than    3878 bp
  65% of sequence in fragments shorter than    4359 bp
  70% of sequence in fragments shorter than    4937 bp
  75% of sequence in fragments shorter than    5652 bp
  80% of sequence in fragments shorter than    6582 bp
  85% of sequence in fragments shorter than    7885 bp
  90% of sequence in fragments shorter than   10036 bp
  95% of sequence in fragments shorter than   15440 bp

# FnuDII.PpuMI (CGCG+RGGWCCY) double digest, 1818 thousand fragments (733940+1085138 sites), 1638 bp average fragment length
   5% of sequence in fragments shorter than     561 bp
  10% of sequence in fragments shorter than     962 bp
  15% of sequence in fragments shorter than    1328 bp
  20% of sequence in fragments shorter than    1689 bp
  25% of sequence in fragments shorter than    2048 bp
  30% of sequence in fragments shorter than    2418 bp
  35% of sequence in fragments shorter than    2807 bp
  40% of sequence in fragments shorter than    3208 bp
  45% of sequence in fragments shorter than    3607 bp
  50% of sequence in fragments shorter than    4057 bp
  55% of sequence in fragments shorter than    4564 bp
  60% of sequence in fragments shorter than    5131 bp
  65% of sequence in fragments shorter than    5777 bp
  70% of sequence in fragments shorter than    6512 bp
  75% of sequence in fragments shorter than    7392 bp
  80% of sequence in fragments shorter than    8473 bp
  85% of sequence in fragments shorter than    9889 bp
  90% of sequence in fragments shorter than   12015 bp
  95% of sequence in fragments shorter than   16245 bp

# FnuDII.PvuII (CGCG+CAGCTG) double digest, 1817 thousand fragments (733940+1084260 sites), 1639 bp average fragment length
   5% of sequence in fragments shorter than     572 bp
  10% of sequence in fragments shorter than     951 bp
  15% of sequence in fragments shorter than    1296 bp
  20% of sequence in fragments shorter than    1632 bp
  25% of sequence in fragments shorter than    1970 bp
  30% of sequence in fragments shorter than    2315 bp
  35% of sequence in fragments shorter than    2675 bp
  40% of sequence in fragments shorter than    3044 bp
  45% of sequence in fragments shorter than    3449 bp
  50% of sequence in fragments shorter than    3889 bp
  55% of sequence in fragments shorter than    4377 bp
  60% of sequence in fragments shorter than    4917 bp
  65% of sequence in fragments shorter than    5526 bp
  70% of sequence in fragments shorter than    6221 bp
  75% of sequence in fragments shorter than    7032 bp
  80% of sequence in fragments shorter than    8031 bp
  85% of sequence in fragments shorter than    9350 bp
  90% of sequence in fragments shorter than   11318 bp
  95% of sequence in fragments shorter than   15076 bp

# ApaLI.PstI (GTGCAC+CTGCAG) double digest, 1817 thousand fragments (496255+1321469 sites), 1639 bp average fragment length
   5% of sequence in fragments shorter than     554 bp
  10% of sequence in fragments shorter than     858 bp
  15% of sequence in fragments shorter than    1135 bp
  20% of sequence in fragments shorter than    1396 bp
  25% of sequence in fragments shorter than    1664 bp
  30% of sequence in fragments shorter than    1931 bp
  35% of sequence in fragments shorter than    2205 bp
  40% of sequence in fragments shorter than    2496 bp
  45% of sequence in fragments shorter than    2805 bp
  50% of sequence in fragments shorter than    3135 bp
  55% of sequence in fragments shorter than    3492 bp
  60% of sequence in fragments shorter than    3891 bp
  65% of sequence in fragments shorter than    4334 bp
  70% of sequence in fragments shorter than    4782 bp
  75% of sequence in fragments shorter than    5345 bp
  80% of sequence in fragments shorter than    6049 bp
  85% of sequence in fragments shorter than    6965 bp
  90% of sequence in fragments shorter than    8291 bp
  95% of sequence in fragments shorter than   10770 bp

# FauI.SapI (CCCGC+GCTCTTC) double digest, 1816 thousand fragments (1439772+377161 sites), 1640 bp average fragment length
   5% of sequence in fragments shorter than     568 bp
  10% of sequence in fragments shorter than     960 bp
  15% of sequence in fragments shorter than    1329 bp
  20% of sequence in fragments shorter than    1681 bp
  25% of sequence in fragments shorter than    2053 bp
  30% of sequence in fragments shorter than    2417 bp
  35% of sequence in fragments shorter than    2819 bp
  40% of sequence in fragments shorter than    3241 bp
  45% of sequence in fragments shorter than    3694 bp
  50% of sequence in fragments shorter than    4188 bp
  55% of sequence in fragments shorter than    4725 bp
  60% of sequence in fragments shorter than    5321 bp
  65% of sequence in fragments shorter than    6003 bp
  70% of sequence in fragments shorter than    6792 bp
  75% of sequence in fragments shorter than    7728 bp
  80% of sequence in fragments shorter than    8871 bp
  85% of sequence in fragments shorter than   10366 bp
  90% of sequence in fragments shorter than   12538 bp
  95% of sequence in fragments shorter than   16771 bp

# FauI.SmaI (CCCGC+CCCGGG) double digest, 1816 thousand fragments (1439772+376940 sites), 1640 bp average fragment length
   5% of sequence in fragments shorter than     551 bp
  10% of sequence in fragments shorter than    1003 bp
  15% of sequence in fragments shorter than    1424 bp
  20% of sequence in fragments shorter than    1873 bp
  25% of sequence in fragments shorter than    2330 bp
  30% of sequence in fragments shorter than    2827 bp
  35% of sequence in fragments shorter than    3359 bp
  40% of sequence in fragments shorter than    3945 bp
  45% of sequence in fragments shorter than    4550 bp
  50% of sequence in fragments shorter than    5250 bp
  55% of sequence in fragments shorter than    6039 bp
  60% of sequence in fragments shorter than    6914 bp
  65% of sequence in fragments shorter than    7915 bp
  70% of sequence in fragments shorter than    9076 bp
  75% of sequence in fragments shorter than   10464 bp
  80% of sequence in fragments shorter than   12195 bp
  85% of sequence in fragments shorter than   14490 bp
  90% of sequence in fragments shorter than   17858 bp
  95% of sequence in fragments shorter than   24294 bp

# HhaI.PspXI (GCGC+VCTCGAGB) double digest, 1815 thousand fragments (1756502+59193 sites), 1641 bp average fragment length
   5% of sequence in fragments shorter than     548 bp
  10% of sequence in fragments shorter than     965 bp
  15% of sequence in fragments shorter than    1381 bp
  20% of sequence in fragments shorter than    1797 bp
  25% of sequence in fragments shorter than    2244 bp
  30% of sequence in fragments shorter than    2716 bp
  35% of sequence in fragments shorter than    3217 bp
  40% of sequence in fragments shorter than    3757 bp
  45% of sequence in fragments shorter than    4336 bp
  50% of sequence in fragments shorter than    4989 bp
  55% of sequence in fragments shorter than    5700 bp
  60% of sequence in fragments shorter than    6495 bp
  65% of sequence in fragments shorter than    7401 bp
  70% of sequence in fragments shorter than    8453 bp
  75% of sequence in fragments shorter than    9702 bp
  80% of sequence in fragments shorter than   11252 bp
  85% of sequence in fragments shorter than   13261 bp
  90% of sequence in fragments shorter than   16091 bp
  95% of sequence in fragments shorter than   21103 bp

# BspMI.SapI (ACCTGC+GCTCTTC) double digest, 1814 thousand fragments (1438129+377161 sites), 1642 bp average fragment length
   5% of sequence in fragments shorter than     551 bp
  10% of sequence in fragments shorter than     843 bp
  15% of sequence in fragments shorter than    1113 bp
  20% of sequence in fragments shorter than    1376 bp
  25% of sequence in fragments shorter than    1629 bp
  30% of sequence in fragments shorter than    1894 bp
  35% of sequence in fragments shorter than    2158 bp
  40% of sequence in fragments shorter than    2439 bp
  45% of sequence in fragments shorter than    2735 bp
  50% of sequence in fragments shorter than    3046 bp
  55% of sequence in fragments shorter than    3344 bp
  60% of sequence in fragments shorter than    3710 bp
  65% of sequence in fragments shorter than    4115 bp
  70% of sequence in fragments shorter than    4581 bp
  75% of sequence in fragments shorter than    5127 bp
  80% of sequence in fragments shorter than    5792 bp
  85% of sequence in fragments shorter than    6639 bp
  90% of sequence in fragments shorter than    7839 bp
  95% of sequence in fragments shorter than    9954 bp

# BspMI.SmaI (ACCTGC+CCCGGG) double digest, 1814 thousand fragments (1438129+376940 sites), 1642 bp average fragment length
   5% of sequence in fragments shorter than     537 bp
  10% of sequence in fragments shorter than     852 bp
  15% of sequence in fragments shorter than    1127 bp
  20% of sequence in fragments shorter than    1393 bp
  25% of sequence in fragments shorter than    1672 bp
  30% of sequence in fragments shorter than    1953 bp
  35% of sequence in fragments shorter than    2217 bp
  40% of sequence in fragments shorter than    2518 bp
  45% of sequence in fragments shorter than    2832 bp
  50% of sequence in fragments shorter than    3176 bp
  55% of sequence in fragments shorter than    3498 bp
  60% of sequence in fragments shorter than    3903 bp
  65% of sequence in fragments shorter than    4359 bp
  70% of sequence in fragments shorter than    4880 bp
  75% of sequence in fragments shorter than    5482 bp
  80% of sequence in fragments shorter than    6219 bp
  85% of sequence in fragments shorter than    7174 bp
  90% of sequence in fragments shorter than    8528 bp
  95% of sequence in fragments shorter than   10899 bp

# AccI.BsaAI (GTMKAC+YACGTR) double digest, 1814 thousand fragments (1101063+713801 sites), 1642 bp average fragment length
   5% of sequence in fragments shorter than     587 bp
  10% of sequence in fragments shorter than     899 bp
  15% of sequence in fragments shorter than    1159 bp
  20% of sequence in fragments shorter than    1397 bp
  25% of sequence in fragments shorter than    1643 bp
  30% of sequence in fragments shorter than    1882 bp
  35% of sequence in fragments shorter than    2118 bp
  40% of sequence in fragments shorter than    2370 bp
  45% of sequence in fragments shorter than    2629 bp
  50% of sequence in fragments shorter than    2906 bp
  55% of sequence in fragments shorter than    3198 bp
  60% of sequence in fragments shorter than    3509 bp
  65% of sequence in fragments shorter than    3864 bp
  70% of sequence in fragments shorter than    4260 bp
  75% of sequence in fragments shorter than    4714 bp
  80% of sequence in fragments shorter than    5263 bp
  85% of sequence in fragments shorter than    5960 bp
  90% of sequence in fragments shorter than    6964 bp
  95% of sequence in fragments shorter than    8688 bp

# AgeI.HhaI (ACCGGT+GCGC) double digest, 1809 thousand fragments (53386+1756502 sites), 1646 bp average fragment length
   5% of sequence in fragments shorter than     552 bp
  10% of sequence in fragments shorter than     969 bp
  15% of sequence in fragments shorter than    1385 bp
  20% of sequence in fragments shorter than    1800 bp
  25% of sequence in fragments shorter than    2247 bp
  30% of sequence in fragments shorter than    2716 bp
  35% of sequence in fragments shorter than    3217 bp
  40% of sequence in fragments shorter than    3755 bp
  45% of sequence in fragments shorter than    4319 bp
  50% of sequence in fragments shorter than    4963 bp
  55% of sequence in fragments shorter than    5671 bp
  60% of sequence in fragments shorter than    6456 bp
  65% of sequence in fragments shorter than    7352 bp
  70% of sequence in fragments shorter than    8394 bp
  75% of sequence in fragments shorter than    9628 bp
  80% of sequence in fragments shorter than   11130 bp
  85% of sequence in fragments shorter than   13132 bp
  90% of sequence in fragments shorter than   15919 bp
  95% of sequence in fragments shorter than   20809 bp

# PacI.PsiI (TTAATTAA+TTATAA) double digest, 1806 thousand fragments (159455+1647912 sites), 1649 bp average fragment length
   5% of sequence in fragments shorter than     550 bp
  10% of sequence in fragments shorter than     859 bp
  15% of sequence in fragments shorter than    1136 bp
  20% of sequence in fragments shorter than    1401 bp
  25% of sequence in fragments shorter than    1671 bp
  30% of sequence in fragments shorter than    1946 bp
  35% of sequence in fragments shorter than    2232 bp
  40% of sequence in fragments shorter than    2538 bp
  45% of sequence in fragments shorter than    2864 bp
  50% of sequence in fragments shorter than    3199 bp
  55% of sequence in fragments shorter than    3589 bp
  60% of sequence in fragments shorter than    4027 bp
  65% of sequence in fragments shorter than    4539 bp
  70% of sequence in fragments shorter than    5165 bp
  75% of sequence in fragments shorter than    5939 bp
  80% of sequence in fragments shorter than    6954 bp
  85% of sequence in fragments shorter than    8426 bp
  90% of sequence in fragments shorter than   11045 bp
  95% of sequence in fragments shorter than   18230 bp

# AccI.BceAI (GTMKAC+ACGGC) double digest, 1805 thousand fragments (1101063+705178 sites), 1650 bp average fragment length
   5% of sequence in fragments shorter than     576 bp
  10% of sequence in fragments shorter than     884 bp
  15% of sequence in fragments shorter than    1155 bp
  20% of sequence in fragments shorter than    1398 bp
  25% of sequence in fragments shorter than    1645 bp
  30% of sequence in fragments shorter than    1891 bp
  35% of sequence in fragments shorter than    2139 bp
  40% of sequence in fragments shorter than    2398 bp
  45% of sequence in fragments shorter than    2667 bp
  50% of sequence in fragments shorter than    2948 bp
  55% of sequence in fragments shorter than    3256 bp
  60% of sequence in fragments shorter than    3585 bp
  65% of sequence in fragments shorter than    3956 bp
  70% of sequence in fragments shorter than    4373 bp
  75% of sequence in fragments shorter than    4851 bp
  80% of sequence in fragments shorter than    5396 bp
  85% of sequence in fragments shorter than    6087 bp
  90% of sequence in fragments shorter than    7094 bp
  95% of sequence in fragments shorter than    8893 bp

# Hpy99I.PsiI (CGWCG+TTATAA) double digest, 1805 thousand fragments (157917+1647912 sites), 1650 bp average fragment length
   5% of sequence in fragments shorter than     560 bp
  10% of sequence in fragments shorter than     873 bp
  15% of sequence in fragments shorter than    1149 bp
  20% of sequence in fragments shorter than    1410 bp
  25% of sequence in fragments shorter than    1673 bp
  30% of sequence in fragments shorter than    1937 bp
  35% of sequence in fragments shorter than    2211 bp
  40% of sequence in fragments shorter than    2498 bp
  45% of sequence in fragments shorter than    2794 bp
  50% of sequence in fragments shorter than    3103 bp
  55% of sequence in fragments shorter than    3457 bp
  60% of sequence in fragments shorter than    3847 bp
  65% of sequence in fragments shorter than    4289 bp
  70% of sequence in fragments shorter than    4811 bp
  75% of sequence in fragments shorter than    5439 bp
  80% of sequence in fragments shorter than    6215 bp
  85% of sequence in fragments shorter than    7257 bp
  90% of sequence in fragments shorter than    8826 bp
  95% of sequence in fragments shorter than   12215 bp

# BamHI.FauI (GGATCC+CCCGC) double digest, 1805 thousand fragments (365999+1439772 sites), 1650 bp average fragment length
   5% of sequence in fragments shorter than     561 bp
  10% of sequence in fragments shorter than     973 bp
  15% of sequence in fragments shorter than    1352 bp
  20% of sequence in fragments shorter than    1722 bp
  25% of sequence in fragments shorter than    2097 bp
  30% of sequence in fragments shorter than    2503 bp
  35% of sequence in fragments shorter than    2926 bp
  40% of sequence in fragments shorter than    3371 bp
  45% of sequence in fragments shorter than    3862 bp
  50% of sequence in fragments shorter than    4364 bp
  55% of sequence in fragments shorter than    4913 bp
  60% of sequence in fragments shorter than    5553 bp
  65% of sequence in fragments shorter than    6276 bp
  70% of sequence in fragments shorter than    7115 bp
  75% of sequence in fragments shorter than    8112 bp
  80% of sequence in fragments shorter than    9352 bp
  85% of sequence in fragments shorter than   10965 bp
  90% of sequence in fragments shorter than   13266 bp
  95% of sequence in fragments shorter than   17676 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# BamHI.BspMI (GGATCC+ACCTGC) double digest, 1803 thousand fragments (365999+1438129 sites), 1652 bp average fragment length
   5% of sequence in fragments shorter than     557 bp
  10% of sequence in fragments shorter than     862 bp
  15% of sequence in fragments shorter than    1137 bp
  20% of sequence in fragments shorter than    1380 bp
  25% of sequence in fragments shorter than    1634 bp
  30% of sequence in fragments shorter than    1901 bp
  35% of sequence in fragments shorter than    2135 bp
  40% of sequence in fragments shorter than    2416 bp
  45% of sequence in fragments shorter than    2716 bp
  50% of sequence in fragments shorter than    3030 bp
  55% of sequence in fragments shorter than    3364 bp
  60% of sequence in fragments shorter than    3745 bp
  65% of sequence in fragments shorter than    4159 bp
  70% of sequence in fragments shorter than    4641 bp
  75% of sequence in fragments shorter than    5196 bp
  80% of sequence in fragments shorter than    5875 bp
  85% of sequence in fragments shorter than    6745 bp
  90% of sequence in fragments shorter than    7985 bp
  95% of sequence in fragments shorter than   10145 bp

# BscGI.HgaI (CCCGT+GACGC) double digest, 1802 thousand fragments (1231070+571889 sites), 1653 bp average fragment length
   5% of sequence in fragments shorter than     469 bp
  10% of sequence in fragments shorter than     828 bp
  15% of sequence in fragments shorter than    1178 bp
  20% of sequence in fragments shorter than    1525 bp
  25% of sequence in fragments shorter than    1878 bp
  30% of sequence in fragments shorter than    2246 bp
  35% of sequence in fragments shorter than    2634 bp
  40% of sequence in fragments shorter than    3045 bp
  45% of sequence in fragments shorter than    3483 bp
  50% of sequence in fragments shorter than    3951 bp
  55% of sequence in fragments shorter than    4469 bp
  60% of sequence in fragments shorter than    5045 bp
  65% of sequence in fragments shorter than    5698 bp
  70% of sequence in fragments shorter than    6442 bp
  75% of sequence in fragments shorter than    7308 bp
  80% of sequence in fragments shorter than    8389 bp
  85% of sequence in fragments shorter than    9784 bp
  90% of sequence in fragments shorter than   11748 bp
  95% of sequence in fragments shorter than   15113 bp

# BscGI.EciI (CCCGT+GGCGGA) double digest, 1801 thousand fragments (1231070+571273 sites), 1653 bp average fragment length
   5% of sequence in fragments shorter than     474 bp
  10% of sequence in fragments shorter than     842 bp
  15% of sequence in fragments shorter than    1194 bp
  20% of sequence in fragments shorter than    1548 bp
  25% of sequence in fragments shorter than    1916 bp
  30% of sequence in fragments shorter than    2300 bp
  35% of sequence in fragments shorter than    2706 bp
  40% of sequence in fragments shorter than    3145 bp
  45% of sequence in fragments shorter than    3615 bp
  50% of sequence in fragments shorter than    4131 bp
  55% of sequence in fragments shorter than    4699 bp
  60% of sequence in fragments shorter than    5335 bp
  65% of sequence in fragments shorter than    6060 bp
  70% of sequence in fragments shorter than    6877 bp
  75% of sequence in fragments shorter than    7855 bp
  80% of sequence in fragments shorter than    9065 bp
  85% of sequence in fragments shorter than   10624 bp
  90% of sequence in fragments shorter than   12857 bp
  95% of sequence in fragments shorter than   16676 bp

# BsaAI.PpuMI (YACGTR+RGGWCCY) double digest, 1798 thousand fragments (713801+1085138 sites), 1656 bp average fragment length
   5% of sequence in fragments shorter than     556 bp
  10% of sequence in fragments shorter than     879 bp
  15% of sequence in fragments shorter than    1155 bp
  20% of sequence in fragments shorter than    1412 bp
  25% of sequence in fragments shorter than    1684 bp
  30% of sequence in fragments shorter than    1957 bp
  35% of sequence in fragments shorter than    2228 bp
  40% of sequence in fragments shorter than    2526 bp
  45% of sequence in fragments shorter than    2839 bp
  50% of sequence in fragments shorter than    3163 bp
  55% of sequence in fragments shorter than    3518 bp
  60% of sequence in fragments shorter than    3907 bp
  65% of sequence in fragments shorter than    4351 bp
  70% of sequence in fragments shorter than    4857 bp
  75% of sequence in fragments shorter than    5443 bp
  80% of sequence in fragments shorter than    6158 bp
  85% of sequence in fragments shorter than    7078 bp
  90% of sequence in fragments shorter than    8412 bp
  95% of sequence in fragments shorter than   10895 bp

# HhaI.PmeI (GCGC+GTTTAAAC) double digest, 1798 thousand fragments (1756502+42094 sites), 1657 bp average fragment length
   5% of sequence in fragments shorter than     557 bp
  10% of sequence in fragments shorter than     978 bp
  15% of sequence in fragments shorter than    1393 bp
  20% of sequence in fragments shorter than    1812 bp
  25% of sequence in fragments shorter than    2256 bp
  30% of sequence in fragments shorter than    2721 bp
  35% of sequence in fragments shorter than    3216 bp
  40% of sequence in fragments shorter than    3748 bp
  45% of sequence in fragments shorter than    4310 bp
  50% of sequence in fragments shorter than    4947 bp
  55% of sequence in fragments shorter than    5641 bp
  60% of sequence in fragments shorter than    6407 bp
  65% of sequence in fragments shorter than    7282 bp
  70% of sequence in fragments shorter than    8298 bp
  75% of sequence in fragments shorter than    9508 bp
  80% of sequence in fragments shorter than   10983 bp
  85% of sequence in fragments shorter than   12912 bp
  90% of sequence in fragments shorter than   15610 bp
  95% of sequence in fragments shorter than   20422 bp

# BsaAI.PvuII (YACGTR+CAGCTG) double digest, 1797 thousand fragments (713801+1084260 sites), 1657 bp average fragment length
   5% of sequence in fragments shorter than     566 bp
  10% of sequence in fragments shorter than     877 bp
  15% of sequence in fragments shorter than    1142 bp
  20% of sequence in fragments shorter than    1396 bp
  25% of sequence in fragments shorter than    1655 bp
  30% of sequence in fragments shorter than    1913 bp
  35% of sequence in fragments shorter than    2173 bp
  40% of sequence in fragments shorter than    2449 bp
  45% of sequence in fragments shorter than    2744 bp
  50% of sequence in fragments shorter than    3049 bp
  55% of sequence in fragments shorter than    3388 bp
  60% of sequence in fragments shorter than    3739 bp
  65% of sequence in fragments shorter than    4149 bp
  70% of sequence in fragments shorter than    4616 bp
  75% of sequence in fragments shorter than    5154 bp
  80% of sequence in fragments shorter than    5816 bp
  85% of sequence in fragments shorter than    6673 bp
  90% of sequence in fragments shorter than    7893 bp
  95% of sequence in fragments shorter than   10101 bp

# AccI.BciVI (GTMKAC+GTATCC) double digest, 1796 thousand fragments (1101063+696093 sites), 1658 bp average fragment length
   5% of sequence in fragments shorter than     589 bp
  10% of sequence in fragments shorter than     892 bp
  15% of sequence in fragments shorter than    1152 bp
  20% of sequence in fragments shorter than    1388 bp
  25% of sequence in fragments shorter than    1615 bp
  30% of sequence in fragments shorter than    1834 bp
  35% of sequence in fragments shorter than    2073 bp
  40% of sequence in fragments shorter than    2319 bp
  45% of sequence in fragments shorter than    2577 bp
  50% of sequence in fragments shorter than    2851 bp
  55% of sequence in fragments shorter than    3150 bp
  60% of sequence in fragments shorter than    3473 bp
  65% of sequence in fragments shorter than    3829 bp
  70% of sequence in fragments shorter than    4233 bp
  75% of sequence in fragments shorter than    4699 bp
  80% of sequence in fragments shorter than    5283 bp
  85% of sequence in fragments shorter than    6030 bp
  90% of sequence in fragments shorter than    7110 bp
  95% of sequence in fragments shorter than    9043 bp

# BscGI.MfeI (CCCGT+CAATTG) double digest, 1794 thousand fragments (1231070+564303 sites), 1660 bp average fragment length
   5% of sequence in fragments shorter than     518 bp
  10% of sequence in fragments shorter than     847 bp
  15% of sequence in fragments shorter than    1147 bp
  20% of sequence in fragments shorter than    1432 bp
  25% of sequence in fragments shorter than    1710 bp
  30% of sequence in fragments shorter than    1991 bp
  35% of sequence in fragments shorter than    2272 bp
  40% of sequence in fragments shorter than    2566 bp
  45% of sequence in fragments shorter than    2872 bp
  50% of sequence in fragments shorter than    3191 bp
  55% of sequence in fragments shorter than    3533 bp
  60% of sequence in fragments shorter than    3909 bp
  65% of sequence in fragments shorter than    4327 bp
  70% of sequence in fragments shorter than    4797 bp
  75% of sequence in fragments shorter than    5346 bp
  80% of sequence in fragments shorter than    6009 bp
  85% of sequence in fragments shorter than    6854 bp
  90% of sequence in fragments shorter than    8007 bp
  95% of sequence in fragments shorter than    9990 bp

# AvaIII.HindIII (ATGCAT+AAGCTT) double digest, 1793 thousand fragments (933357+860365 sites), 1661 bp average fragment length
   5% of sequence in fragments shorter than     577 bp
  10% of sequence in fragments shorter than     885 bp
  15% of sequence in fragments shorter than    1147 bp
  20% of sequence in fragments shorter than    1392 bp
  25% of sequence in fragments shorter than    1638 bp
  30% of sequence in fragments shorter than    1881 bp
  35% of sequence in fragments shorter than    2105 bp
  40% of sequence in fragments shorter than    2362 bp
  45% of sequence in fragments shorter than    2627 bp
  50% of sequence in fragments shorter than    2910 bp
  55% of sequence in fragments shorter than    3214 bp
  60% of sequence in fragments shorter than    3551 bp
  65% of sequence in fragments shorter than    3928 bp
  70% of sequence in fragments shorter than    4356 bp
  75% of sequence in fragments shorter than    4856 bp
  80% of sequence in fragments shorter than    5480 bp
  85% of sequence in fragments shorter than    6302 bp
  90% of sequence in fragments shorter than    7520 bp
  95% of sequence in fragments shorter than    9945 bp

# BfiI.ClaI (ACTGGG+ATCGAT) double digest, 1792 thousand fragments (1706594+86477 sites), 1662 bp average fragment length
   5% of sequence in fragments shorter than     566 bp
  10% of sequence in fragments shorter than     871 bp
  15% of sequence in fragments shorter than    1134 bp
  20% of sequence in fragments shorter than    1390 bp
  25% of sequence in fragments shorter than    1643 bp
  30% of sequence in fragments shorter than    1890 bp
  35% of sequence in fragments shorter than    2133 bp
  40% of sequence in fragments shorter than    2391 bp
  45% of sequence in fragments shorter than    2671 bp
  50% of sequence in fragments shorter than    2950 bp
  55% of sequence in fragments shorter than    3273 bp
  60% of sequence in fragments shorter than    3626 bp
  65% of sequence in fragments shorter than    4022 bp
  70% of sequence in fragments shorter than    4471 bp
  75% of sequence in fragments shorter than    4986 bp
  80% of sequence in fragments shorter than    5621 bp
  85% of sequence in fragments shorter than    6450 bp
  90% of sequence in fragments shorter than    7662 bp
  95% of sequence in fragments shorter than   10001 bp

# BceAI.PpuMI (ACGGC+RGGWCCY) double digest, 1789 thousand fragments (705178+1085138 sites), 1664 bp average fragment length
   5% of sequence in fragments shorter than     526 bp
  10% of sequence in fragments shorter than     871 bp
  15% of sequence in fragments shorter than    1191 bp
  20% of sequence in fragments shorter than    1508 bp
  25% of sequence in fragments shorter than    1827 bp
  30% of sequence in fragments shorter than    2149 bp
  35% of sequence in fragments shorter than    2489 bp
  40% of sequence in fragments shorter than    2845 bp
  45% of sequence in fragments shorter than    3217 bp
  50% of sequence in fragments shorter than    3594 bp
  55% of sequence in fragments shorter than    4023 bp
  60% of sequence in fragments shorter than    4511 bp
  65% of sequence in fragments shorter than    5067 bp
  70% of sequence in fragments shorter than    5698 bp
  75% of sequence in fragments shorter than    6443 bp
  80% of sequence in fragments shorter than    7357 bp
  85% of sequence in fragments shorter than    8546 bp
  90% of sequence in fragments shorter than   10281 bp
  95% of sequence in fragments shorter than   13567 bp

# BceAI.PvuII (ACGGC+CAGCTG) double digest, 1788 thousand fragments (705178+1084260 sites), 1665 bp average fragment length
   5% of sequence in fragments shorter than     539 bp
  10% of sequence in fragments shorter than     870 bp
  15% of sequence in fragments shorter than    1168 bp
  20% of sequence in fragments shorter than    1460 bp
  25% of sequence in fragments shorter than    1757 bp
  30% of sequence in fragments shorter than    2061 bp
  35% of sequence in fragments shorter than    2375 bp
  40% of sequence in fragments shorter than    2705 bp
  45% of sequence in fragments shorter than    3051 bp
  50% of sequence in fragments shorter than    3433 bp
  55% of sequence in fragments shorter than    3850 bp
  60% of sequence in fragments shorter than    4314 bp
  65% of sequence in fragments shorter than    4833 bp
  70% of sequence in fragments shorter than    5431 bp
  75% of sequence in fragments shorter than    6131 bp
  80% of sequence in fragments shorter than    6965 bp
  85% of sequence in fragments shorter than    8065 bp
  90% of sequence in fragments shorter than    9667 bp
  95% of sequence in fragments shorter than   12692 bp

# HhaI.SalI (GCGC+GTCGAC) double digest, 1787 thousand fragments (1756502+31396 sites), 1667 bp average fragment length
   5% of sequence in fragments shorter than     559 bp
  10% of sequence in fragments shorter than     984 bp
  15% of sequence in fragments shorter than    1407 bp
  20% of sequence in fragments shorter than    1836 bp
  25% of sequence in fragments shorter than    2291 bp
  30% of sequence in fragments shorter than    2768 bp
  35% of sequence in fragments shorter than    3279 bp
  40% of sequence in fragments shorter than    3827 bp
  45% of sequence in fragments shorter than    4416 bp
  50% of sequence in fragments shorter than    5070 bp
  55% of sequence in fragments shorter than    5793 bp
  60% of sequence in fragments shorter than    6596 bp
  65% of sequence in fragments shorter than    7507 bp
  70% of sequence in fragments shorter than    8573 bp
  75% of sequence in fragments shorter than    9848 bp
  80% of sequence in fragments shorter than   11412 bp
  85% of sequence in fragments shorter than   13460 bp
  90% of sequence in fragments shorter than   16331 bp
  95% of sequence in fragments shorter than   21364 bp

# BsrBI.HgiCI (CCGCTC+GGYRCC) double digest, 1785 thousand fragments (235523+1550877 sites), 1668 bp average fragment length
   5% of sequence in fragments shorter than     544 bp
  10% of sequence in fragments shorter than     863 bp
  15% of sequence in fragments shorter than    1164 bp
  20% of sequence in fragments shorter than    1430 bp
  25% of sequence in fragments shorter than    1712 bp
  30% of sequence in fragments shorter than    2008 bp
  35% of sequence in fragments shorter than    2316 bp
  40% of sequence in fragments shorter than    2644 bp
  45% of sequence in fragments shorter than    2979 bp
  50% of sequence in fragments shorter than    3356 bp
  55% of sequence in fragments shorter than    3771 bp
  60% of sequence in fragments shorter than    4226 bp
  65% of sequence in fragments shorter than    4737 bp
  70% of sequence in fragments shorter than    5338 bp
  75% of sequence in fragments shorter than    6040 bp
  80% of sequence in fragments shorter than    6921 bp
  85% of sequence in fragments shorter than    8083 bp
  90% of sequence in fragments shorter than    9789 bp
  95% of sequence in fragments shorter than   13148 bp

# HindIII.StuI (AAGCTT+AGGCCT) double digest, 1785 thousand fragments (860365+925729 sites), 1668 bp average fragment length
   5% of sequence in fragments shorter than     550 bp
  10% of sequence in fragments shorter than     859 bp
  15% of sequence in fragments shorter than    1150 bp
  20% of sequence in fragments shorter than    1410 bp
  25% of sequence in fragments shorter than    1683 bp
  30% of sequence in fragments shorter than    1928 bp
  35% of sequence in fragments shorter than    2197 bp
  40% of sequence in fragments shorter than    2476 bp
  45% of sequence in fragments shorter than    2766 bp
  50% of sequence in fragments shorter than    3070 bp
  55% of sequence in fragments shorter than    3396 bp
  60% of sequence in fragments shorter than    3756 bp
  65% of sequence in fragments shorter than    4143 bp
  70% of sequence in fragments shorter than    4585 bp
  75% of sequence in fragments shorter than    5091 bp
  80% of sequence in fragments shorter than    5704 bp
  85% of sequence in fragments shorter than    6477 bp
  90% of sequence in fragments shorter than    7542 bp
  95% of sequence in fragments shorter than    9374 bp

# BsmI.BsrBI (GAATGC+CCGCTC) double digest, 1784 thousand fragments (1549350+235523 sites), 1670 bp average fragment length
   5% of sequence in fragments shorter than     566 bp
  10% of sequence in fragments shorter than     989 bp
  15% of sequence in fragments shorter than    1317 bp
  20% of sequence in fragments shorter than    1632 bp
  25% of sequence in fragments shorter than    1935 bp
  30% of sequence in fragments shorter than    2236 bp
  35% of sequence in fragments shorter than    2534 bp
  40% of sequence in fragments shorter than    2841 bp
  45% of sequence in fragments shorter than    3161 bp
  50% of sequence in fragments shorter than    3503 bp
  55% of sequence in fragments shorter than    3868 bp
  60% of sequence in fragments shorter than    4260 bp
  65% of sequence in fragments shorter than    4695 bp
  70% of sequence in fragments shorter than    5184 bp
  75% of sequence in fragments shorter than    5751 bp
  80% of sequence in fragments shorter than    6423 bp
  85% of sequence in fragments shorter than    7261 bp
  90% of sequence in fragments shorter than    8425 bp
  95% of sequence in fragments shorter than   10405 bp

# AvaIII.XbaI (ATGCAT+TCTAGA) double digest, 1783 thousand fragments (933357+850998 sites), 1670 bp average fragment length
   5% of sequence in fragments shorter than     591 bp
  10% of sequence in fragments shorter than     878 bp
  15% of sequence in fragments shorter than    1145 bp
  20% of sequence in fragments shorter than    1387 bp
  25% of sequence in fragments shorter than    1628 bp
  30% of sequence in fragments shorter than    1872 bp
  35% of sequence in fragments shorter than    2112 bp
  40% of sequence in fragments shorter than    2362 bp
  45% of sequence in fragments shorter than    2636 bp
  50% of sequence in fragments shorter than    2931 bp
  55% of sequence in fragments shorter than    3245 bp
  60% of sequence in fragments shorter than    3590 bp
  65% of sequence in fragments shorter than    3970 bp
  70% of sequence in fragments shorter than    4404 bp
  75% of sequence in fragments shorter than    4912 bp
  80% of sequence in fragments shorter than    5530 bp
  85% of sequence in fragments shorter than    6343 bp
  90% of sequence in fragments shorter than    7526 bp
  95% of sequence in fragments shorter than    9769 bp

# AflIII.SexAI (ACRYGT+ACCWGGT) double digest, 1782 thousand fragments (1485394+297900 sites), 1671 bp average fragment length
   5% of sequence in fragments shorter than     599 bp
  10% of sequence in fragments shorter than     928 bp
  15% of sequence in fragments shorter than    1210 bp
  20% of sequence in fragments shorter than    1473 bp
  25% of sequence in fragments shorter than    1729 bp
  30% of sequence in fragments shorter than    1979 bp
  35% of sequence in fragments shorter than    2232 bp
  40% of sequence in fragments shorter than    2496 bp
  45% of sequence in fragments shorter than    2769 bp
  50% of sequence in fragments shorter than    3059 bp
  55% of sequence in fragments shorter than    3373 bp
  60% of sequence in fragments shorter than    3718 bp
  65% of sequence in fragments shorter than    4100 bp
  70% of sequence in fragments shorter than    4527 bp
  75% of sequence in fragments shorter than    5032 bp
  80% of sequence in fragments shorter than    5642 bp
  85% of sequence in fragments shorter than    6386 bp
  90% of sequence in fragments shorter than    7453 bp
  95% of sequence in fragments shorter than    9452 bp

# BscGI.SphI (CCCGT+GCATGC) double digest, 1781 thousand fragments (1231070+550952 sites), 1672 bp average fragment length
   5% of sequence in fragments shorter than     506 bp
  10% of sequence in fragments shorter than     836 bp
  15% of sequence in fragments shorter than    1145 bp
  20% of sequence in fragments shorter than    1445 bp
  25% of sequence in fragments shorter than    1747 bp
  30% of sequence in fragments shorter than    2057 bp
  35% of sequence in fragments shorter than    2380 bp
  40% of sequence in fragments shorter than    2718 bp
  45% of sequence in fragments shorter than    3079 bp
  50% of sequence in fragments shorter than    3469 bp
  55% of sequence in fragments shorter than    3890 bp
  60% of sequence in fragments shorter than    4356 bp
  65% of sequence in fragments shorter than    4882 bp
  70% of sequence in fragments shorter than    5483 bp
  75% of sequence in fragments shorter than    6179 bp
  80% of sequence in fragments shorter than    7017 bp
  85% of sequence in fragments shorter than    8105 bp
  90% of sequence in fragments shorter than    9650 bp
  95% of sequence in fragments shorter than   12242 bp

# ApaI.PstI (GGGCCC+CTGCAG) double digest, 1781 thousand fragments (460339+1321469 sites), 1672 bp average fragment length
   5% of sequence in fragments shorter than     537 bp
  10% of sequence in fragments shorter than     861 bp
  15% of sequence in fragments shorter than    1169 bp
  20% of sequence in fragments shorter than    1464 bp
  25% of sequence in fragments shorter than    1775 bp
  30% of sequence in fragments shorter than    2086 bp
  35% of sequence in fragments shorter than    2417 bp
  40% of sequence in fragments shorter than    2765 bp
  45% of sequence in fragments shorter than    3141 bp
  50% of sequence in fragments shorter than    3543 bp
  55% of sequence in fragments shorter than    3988 bp
  60% of sequence in fragments shorter than    4485 bp
  65% of sequence in fragments shorter than    5031 bp
  70% of sequence in fragments shorter than    5654 bp
  75% of sequence in fragments shorter than    6378 bp
  80% of sequence in fragments shorter than    7264 bp
  85% of sequence in fragments shorter than    8422 bp
  90% of sequence in fragments shorter than   10110 bp
  95% of sequence in fragments shorter than   13289 bp

# BciVI.PpuMI (GTATCC+RGGWCCY) double digest, 1780 thousand fragments (696093+1085138 sites), 1673 bp average fragment length
   5% of sequence in fragments shorter than     570 bp
  10% of sequence in fragments shorter than     891 bp
  15% of sequence in fragments shorter than    1172 bp
  20% of sequence in fragments shorter than    1441 bp
  25% of sequence in fragments shorter than    1699 bp
  30% of sequence in fragments shorter than    1950 bp
  35% of sequence in fragments shorter than    2218 bp
  40% of sequence in fragments shorter than    2501 bp
  45% of sequence in fragments shorter than    2800 bp
  50% of sequence in fragments shorter than    3113 bp
  55% of sequence in fragments shorter than    3447 bp
  60% of sequence in fragments shorter than    3812 bp
  65% of sequence in fragments shorter than    4223 bp
  70% of sequence in fragments shorter than    4700 bp
  75% of sequence in fragments shorter than    5263 bp
  80% of sequence in fragments shorter than    5936 bp
  85% of sequence in fragments shorter than    6826 bp
  90% of sequence in fragments shorter than    8111 bp
  95% of sequence in fragments shorter than   10525 bp

# AvaIII.EcoRI (ATGCAT+GAATTC) double digest, 1780 thousand fragments (933357+847401 sites), 1673 bp average fragment length
   5% of sequence in fragments shorter than     575 bp
  10% of sequence in fragments shorter than     875 bp
  15% of sequence in fragments shorter than    1150 bp
  20% of sequence in fragments shorter than    1404 bp
  25% of sequence in fragments shorter than    1657 bp
  30% of sequence in fragments shorter than    1909 bp
  35% of sequence in fragments shorter than    2159 bp
  40% of sequence in fragments shorter than    2423 bp
  45% of sequence in fragments shorter than    2698 bp
  50% of sequence in fragments shorter than    2991 bp
  55% of sequence in fragments shorter than    3305 bp
  60% of sequence in fragments shorter than    3644 bp
  65% of sequence in fragments shorter than    4024 bp
  70% of sequence in fragments shorter than    4457 bp
  75% of sequence in fragments shorter than    4970 bp
  80% of sequence in fragments shorter than    5596 bp
  85% of sequence in fragments shorter than    6412 bp
  90% of sequence in fragments shorter than    7584 bp
  95% of sequence in fragments shorter than    9730 bp

# AvaI.BglII (CYCGRG+AGATCT) double digest, 1780 thousand fragments (1005714+774732 sites), 1674 bp average fragment length
   5% of sequence in fragments shorter than     570 bp
  10% of sequence in fragments shorter than     882 bp
  15% of sequence in fragments shorter than    1164 bp
  20% of sequence in fragments shorter than    1435 bp
  25% of sequence in fragments shorter than    1701 bp
  30% of sequence in fragments shorter than    1969 bp
  35% of sequence in fragments shorter than    2248 bp
  40% of sequence in fragments shorter than    2540 bp
  45% of sequence in fragments shorter than    2853 bp
  50% of sequence in fragments shorter than    3184 bp
  55% of sequence in fragments shorter than    3541 bp
  60% of sequence in fragments shorter than    3933 bp
  65% of sequence in fragments shorter than    4367 bp
  70% of sequence in fragments shorter than    4863 bp
  75% of sequence in fragments shorter than    5450 bp
  80% of sequence in fragments shorter than    6166 bp
  85% of sequence in fragments shorter than    7117 bp
  90% of sequence in fragments shorter than    8541 bp
  95% of sequence in fragments shorter than   11371 bp

# BciVI.PvuII (GTATCC+CAGCTG) double digest, 1779 thousand fragments (696093+1084260 sites), 1674 bp average fragment length
   5% of sequence in fragments shorter than     583 bp
  10% of sequence in fragments shorter than     891 bp
  15% of sequence in fragments shorter than    1160 bp
  20% of sequence in fragments shorter than    1412 bp
  25% of sequence in fragments shorter than    1665 bp
  30% of sequence in fragments shorter than    1899 bp
  35% of sequence in fragments shorter than    2149 bp
  40% of sequence in fragments shorter than    2410 bp
  45% of sequence in fragments shorter than    2680 bp
  50% of sequence in fragments shorter than    2964 bp
  55% of sequence in fragments shorter than    3274 bp
  60% of sequence in fragments shorter than    3608 bp
  65% of sequence in fragments shorter than    3985 bp
  70% of sequence in fragments shorter than    4411 bp
  75% of sequence in fragments shorter than    4919 bp
  80% of sequence in fragments shorter than    5535 bp
  85% of sequence in fragments shorter than    6324 bp
  90% of sequence in fragments shorter than    7463 bp
  95% of sequence in fragments shorter than    9549 bp

# PsiI.SnaBI (TTATAA+TACGTA) double digest, 1779 thousand fragments (1647912+132299 sites), 1674 bp average fragment length
   5% of sequence in fragments shorter than     558 bp
  10% of sequence in fragments shorter than     872 bp
  15% of sequence in fragments shorter than    1152 bp
  20% of sequence in fragments shorter than    1415 bp
  25% of sequence in fragments shorter than    1686 bp
  30% of sequence in fragments shorter than    1961 bp
  35% of sequence in fragments shorter than    2251 bp
  40% of sequence in fragments shorter than    2557 bp
  45% of sequence in fragments shorter than    2880 bp
  50% of sequence in fragments shorter than    3215 bp
  55% of sequence in fragments shorter than    3599 bp
  60% of sequence in fragments shorter than    4035 bp
  65% of sequence in fragments shorter than    4544 bp
  70% of sequence in fragments shorter than    5161 bp
  75% of sequence in fragments shorter than    5925 bp
  80% of sequence in fragments shorter than    6919 bp
  85% of sequence in fragments shorter than    8327 bp
  90% of sequence in fragments shorter than   10796 bp
  95% of sequence in fragments shorter than   17001 bp

# BsmI.KasI (GAATGC+GGCGCC) double digest, 1779 thousand fragments (1549350+230381 sites), 1674 bp average fragment length
   5% of sequence in fragments shorter than     569 bp
  10% of sequence in fragments shorter than     981 bp
  15% of sequence in fragments shorter than    1310 bp
  20% of sequence in fragments shorter than    1624 bp
  25% of sequence in fragments shorter than    1918 bp
  30% of sequence in fragments shorter than    2212 bp
  35% of sequence in fragments shorter than    2501 bp
  40% of sequence in fragments shorter than    2797 bp
  45% of sequence in fragments shorter than    3108 bp
  50% of sequence in fragments shorter than    3442 bp
  55% of sequence in fragments shorter than    3795 bp
  60% of sequence in fragments shorter than    4178 bp
  65% of sequence in fragments shorter than    4601 bp
  70% of sequence in fragments shorter than    5076 bp
  75% of sequence in fragments shorter than    5622 bp
  80% of sequence in fragments shorter than    6269 bp
  85% of sequence in fragments shorter than    7088 bp
  90% of sequence in fragments shorter than    8214 bp
  95% of sequence in fragments shorter than   10109 bp

# HhaI.MluI (GCGC+ACGCGT) double digest, 1777 thousand fragments (1756502+21415 sites), 1676 bp average fragment length
   5% of sequence in fragments shorter than     562 bp
  10% of sequence in fragments shorter than     992 bp
  15% of sequence in fragments shorter than    1421 bp
  20% of sequence in fragments shorter than    1856 bp
  25% of sequence in fragments shorter than    2319 bp
  30% of sequence in fragments shorter than    2804 bp
  35% of sequence in fragments shorter than    3327 bp
  40% of sequence in fragments shorter than    3885 bp
  45% of sequence in fragments shorter than    4493 bp
  50% of sequence in fragments shorter than    5165 bp
  55% of sequence in fragments shorter than    5903 bp
  60% of sequence in fragments shorter than    6729 bp
  65% of sequence in fragments shorter than    7670 bp
  70% of sequence in fragments shorter than    8762 bp
  75% of sequence in fragments shorter than   10087 bp
  80% of sequence in fragments shorter than   11705 bp
  85% of sequence in fragments shorter than   13789 bp
  90% of sequence in fragments shorter than   16791 bp
  95% of sequence in fragments shorter than   22036 bp

# AatII.BfiI (GACGTC+ACTGGG) double digest, 1777 thousand fragments (71089+1706594 sites), 1676 bp average fragment length
   5% of sequence in fragments shorter than     567 bp
  10% of sequence in fragments shorter than     879 bp
  15% of sequence in fragments shorter than    1147 bp
  20% of sequence in fragments shorter than    1409 bp
  25% of sequence in fragments shorter than    1666 bp
  30% of sequence in fragments shorter than    1920 bp
  35% of sequence in fragments shorter than    2174 bp
  40% of sequence in fragments shorter than    2438 bp
  45% of sequence in fragments shorter than    2729 bp
  50% of sequence in fragments shorter than    3007 bp
  55% of sequence in fragments shorter than    3338 bp
  60% of sequence in fragments shorter than    3704 bp
  65% of sequence in fragments shorter than    4114 bp
  70% of sequence in fragments shorter than    4576 bp
  75% of sequence in fragments shorter than    5107 bp
  80% of sequence in fragments shorter than    5758 bp
  85% of sequence in fragments shorter than    6622 bp
  90% of sequence in fragments shorter than    7893 bp
  95% of sequence in fragments shorter than   10352 bp

# HgiCI.SwaI (GGYRCC+ATTTAAAT) double digest, 1777 thousand fragments (1550877+226602 sites), 1676 bp average fragment length
   5% of sequence in fragments shorter than     563 bp
  10% of sequence in fragments shorter than     872 bp
  15% of sequence in fragments shorter than    1150 bp
  20% of sequence in fragments shorter than    1389 bp
  25% of sequence in fragments shorter than    1635 bp
  30% of sequence in fragments shorter than    1900 bp
  35% of sequence in fragments shorter than    2170 bp
  40% of sequence in fragments shorter than    2451 bp
  45% of sequence in fragments shorter than    2748 bp
  50% of sequence in fragments shorter than    3048 bp
  55% of sequence in fragments shorter than    3386 bp
  60% of sequence in fragments shorter than    3765 bp
  65% of sequence in fragments shorter than    4181 bp
  70% of sequence in fragments shorter than    4656 bp
  75% of sequence in fragments shorter than    5225 bp
  80% of sequence in fragments shorter than    5920 bp
  85% of sequence in fragments shorter than    6839 bp
  90% of sequence in fragments shorter than    8202 bp
  95% of sequence in fragments shorter than   10977 bp

# StuI.XbaI (AGGCCT+TCTAGA) double digest, 1776 thousand fragments (925729+850998 sites), 1677 bp average fragment length
   5% of sequence in fragments shorter than     552 bp
  10% of sequence in fragments shorter than     860 bp
  15% of sequence in fragments shorter than    1152 bp
  20% of sequence in fragments shorter than    1411 bp
  25% of sequence in fragments shorter than    1658 bp
  30% of sequence in fragments shorter than    1922 bp
  35% of sequence in fragments shorter than    2194 bp
  40% of sequence in fragments shorter than    2476 bp
  45% of sequence in fragments shorter than    2771 bp
  50% of sequence in fragments shorter than    3076 bp
  55% of sequence in fragments shorter than    3409 bp
  60% of sequence in fragments shorter than    3768 bp
  65% of sequence in fragments shorter than    4165 bp
  70% of sequence in fragments shorter than    4613 bp
  75% of sequence in fragments shorter than    5130 bp
  80% of sequence in fragments shorter than    5750 bp
  85% of sequence in fragments shorter than    6527 bp
  90% of sequence in fragments shorter than    7599 bp
  95% of sequence in fragments shorter than    9401 bp

# AclI.EsaBC3I (AACGTT+TCGA) double digest, 1775 thousand fragments (172706+1603346 sites), 1678 bp average fragment length
   5% of sequence in fragments shorter than     575 bp
  10% of sequence in fragments shorter than     879 bp
  15% of sequence in fragments shorter than    1146 bp
  20% of sequence in fragments shorter than    1390 bp
  25% of sequence in fragments shorter than    1643 bp
  30% of sequence in fragments shorter than    1897 bp
  35% of sequence in fragments shorter than    2156 bp
  40% of sequence in fragments shorter than    2430 bp
  45% of sequence in fragments shorter than    2718 bp
  50% of sequence in fragments shorter than    3017 bp
  55% of sequence in fragments shorter than    3343 bp
  60% of sequence in fragments shorter than    3699 bp
  65% of sequence in fragments shorter than    4098 bp
  70% of sequence in fragments shorter than    4550 bp
  75% of sequence in fragments shorter than    5073 bp
  80% of sequence in fragments shorter than    5713 bp
  85% of sequence in fragments shorter than    6532 bp
  90% of sequence in fragments shorter than    7670 bp
  95% of sequence in fragments shorter than    9598 bp

# BsmI.SwaI (GAATGC+ATTTAAAT) double digest, 1775 thousand fragments (1549350+226602 sites), 1678 bp average fragment length
   5% of sequence in fragments shorter than     575 bp
  10% of sequence in fragments shorter than     988 bp
  15% of sequence in fragments shorter than    1315 bp
  20% of sequence in fragments shorter than    1628 bp
  25% of sequence in fragments shorter than    1919 bp
  30% of sequence in fragments shorter than    2212 bp
  35% of sequence in fragments shorter than    2500 bp
  40% of sequence in fragments shorter than    2794 bp
  45% of sequence in fragments shorter than    3106 bp
  50% of sequence in fragments shorter than    3439 bp
  55% of sequence in fragments shorter than    3794 bp
  60% of sequence in fragments shorter than    4179 bp
  65% of sequence in fragments shorter than    4607 bp
  70% of sequence in fragments shorter than    5087 bp
  75% of sequence in fragments shorter than    5644 bp
  80% of sequence in fragments shorter than    6308 bp
  85% of sequence in fragments shorter than    7143 bp
  90% of sequence in fragments shorter than    8321 bp
  95% of sequence in fragments shorter than   10322 bp

# BstEII.FauI (GGTNACC+CCCGC) double digest, 1775 thousand fragments (335865+1439772 sites), 1678 bp average fragment length
   5% of sequence in fragments shorter than     576 bp
  10% of sequence in fragments shorter than     991 bp
  15% of sequence in fragments shorter than    1371 bp
  20% of sequence in fragments shorter than    1746 bp
  25% of sequence in fragments shorter than    2134 bp
  30% of sequence in fragments shorter than    2550 bp
  35% of sequence in fragments shorter than    2965 bp
  40% of sequence in fragments shorter than    3417 bp
  45% of sequence in fragments shorter than    3911 bp
  50% of sequence in fragments shorter than    4446 bp
  55% of sequence in fragments shorter than    5031 bp
  60% of sequence in fragments shorter than    5685 bp
  65% of sequence in fragments shorter than    6417 bp
  70% of sequence in fragments shorter than    7265 bp
  75% of sequence in fragments shorter than    8284 bp
  80% of sequence in fragments shorter than    9535 bp
  85% of sequence in fragments shorter than   11179 bp
  90% of sequence in fragments shorter than   13607 bp
  95% of sequence in fragments shorter than   18128 bp

# BscGI.ScaI (CCCGT+AGTACT) double digest, 1774 thousand fragments (1231070+543793 sites), 1679 bp average fragment length
   5% of sequence in fragments shorter than     518 bp
  10% of sequence in fragments shorter than     850 bp
  15% of sequence in fragments shorter than    1148 bp
  20% of sequence in fragments shorter than    1434 bp
  25% of sequence in fragments shorter than    1717 bp
  30% of sequence in fragments shorter than    2001 bp
  35% of sequence in fragments shorter than    2294 bp
  40% of sequence in fragments shorter than    2597 bp
  45% of sequence in fragments shorter than    2918 bp
  50% of sequence in fragments shorter than    3261 bp
  55% of sequence in fragments shorter than    3626 bp
  60% of sequence in fragments shorter than    4029 bp
  65% of sequence in fragments shorter than    4480 bp
  70% of sequence in fragments shorter than    4985 bp
  75% of sequence in fragments shorter than    5577 bp
  80% of sequence in fragments shorter than    6274 bp
  85% of sequence in fragments shorter than    7186 bp
  90% of sequence in fragments shorter than    8442 bp
  95% of sequence in fragments shorter than   10575 bp

# BspMI.BstEII (ACCTGC+GGTNACC) double digest, 1773 thousand fragments (1438129+335865 sites), 1680 bp average fragment length
   5% of sequence in fragments shorter than     572 bp
  10% of sequence in fragments shorter than     872 bp
  15% of sequence in fragments shorter than    1146 bp
  20% of sequence in fragments shorter than    1408 bp
  25% of sequence in fragments shorter than    1683 bp
  30% of sequence in fragments shorter than    1957 bp
  35% of sequence in fragments shorter than    2231 bp
  40% of sequence in fragments shorter than    2522 bp
  45% of sequence in fragments shorter than    2826 bp
  50% of sequence in fragments shorter than    3153 bp
  55% of sequence in fragments shorter than    3462 bp
  60% of sequence in fragments shorter than    3843 bp
  65% of sequence in fragments shorter than    4274 bp
  70% of sequence in fragments shorter than    4752 bp
  75% of sequence in fragments shorter than    5321 bp
  80% of sequence in fragments shorter than    6008 bp
  85% of sequence in fragments shorter than    6888 bp
  90% of sequence in fragments shorter than    8156 bp
  95% of sequence in fragments shorter than   10382 bp

# NaeI.PsiI (GCCGGC+TTATAA) double digest, 1773 thousand fragments (125692+1647912 sites), 1680 bp average fragment length
   5% of sequence in fragments shorter than     571 bp
  10% of sequence in fragments shorter than     888 bp
  15% of sequence in fragments shorter than    1168 bp
  20% of sequence in fragments shorter than    1434 bp
  25% of sequence in fragments shorter than    1701 bp
  30% of sequence in fragments shorter than    1970 bp
  35% of sequence in fragments shorter than    2248 bp
  40% of sequence in fragments shorter than    2542 bp
  45% of sequence in fragments shorter than    2852 bp
  50% of sequence in fragments shorter than    3165 bp
  55% of sequence in fragments shorter than    3524 bp
  60% of sequence in fragments shorter than    3920 bp
  65% of sequence in fragments shorter than    4371 bp
  70% of sequence in fragments shorter than    4899 bp
  75% of sequence in fragments shorter than    5531 bp
  80% of sequence in fragments shorter than    6318 bp
  85% of sequence in fragments shorter than    7372 bp
  90% of sequence in fragments shorter than    8956 bp
  95% of sequence in fragments shorter than   12396 bp

# BfiI.SacII (ACTGGG+CCGCGG) double digest, 1773 thousand fragments (1706594+66901 sites), 1680 bp average fragment length
   5% of sequence in fragments shorter than     565 bp
  10% of sequence in fragments shorter than     882 bp
  15% of sequence in fragments shorter than    1156 bp
  20% of sequence in fragments shorter than    1424 bp
  25% of sequence in fragments shorter than    1688 bp
  30% of sequence in fragments shorter than    1947 bp
  35% of sequence in fragments shorter than    2200 bp
  40% of sequence in fragments shorter than    2478 bp
  45% of sequence in fragments shorter than    2772 bp
  50% of sequence in fragments shorter than    3062 bp
  55% of sequence in fragments shorter than    3402 bp
  60% of sequence in fragments shorter than    3779 bp
  65% of sequence in fragments shorter than    4198 bp
  70% of sequence in fragments shorter than    4674 bp
  75% of sequence in fragments shorter than    5217 bp
  80% of sequence in fragments shorter than    5890 bp
  85% of sequence in fragments shorter than    6774 bp
  90% of sequence in fragments shorter than    8088 bp
  95% of sequence in fragments shorter than   10632 bp

# AflIII.KpnI (ACRYGT+GGTACC) double digest, 1772 thousand fragments (1485394+287974 sites), 1680 bp average fragment length
   5% of sequence in fragments shorter than     610 bp
  10% of sequence in fragments shorter than     929 bp
  15% of sequence in fragments shorter than    1212 bp
  20% of sequence in fragments shorter than    1458 bp
  25% of sequence in fragments shorter than    1707 bp
  30% of sequence in fragments shorter than    1955 bp
  35% of sequence in fragments shorter than    2208 bp
  40% of sequence in fragments shorter than    2474 bp
  45% of sequence in fragments shorter than    2753 bp
  50% of sequence in fragments shorter than    3037 bp
  55% of sequence in fragments shorter than    3353 bp
  60% of sequence in fragments shorter than    3692 bp
  65% of sequence in fragments shorter than    4071 bp
  70% of sequence in fragments shorter than    4500 bp
  75% of sequence in fragments shorter than    4999 bp
  80% of sequence in fragments shorter than    5610 bp
  85% of sequence in fragments shorter than    6394 bp
  90% of sequence in fragments shorter than    7525 bp
  95% of sequence in fragments shorter than    9657 bp

# EcoRI.StuI (GAATTC+AGGCCT) double digest, 1772 thousand fragments (847401+925729 sites), 1681 bp average fragment length
   5% of sequence in fragments shorter than     546 bp
  10% of sequence in fragments shorter than     873 bp
  15% of sequence in fragments shorter than    1180 bp
  20% of sequence in fragments shorter than    1451 bp
  25% of sequence in fragments shorter than    1709 bp
  30% of sequence in fragments shorter than    1984 bp
  35% of sequence in fragments shorter than    2263 bp
  40% of sequence in fragments shorter than    2549 bp
  45% of sequence in fragments shorter than    2844 bp
  50% of sequence in fragments shorter than    3154 bp
  55% of sequence in fragments shorter than    3487 bp
  60% of sequence in fragments shorter than    3847 bp
  65% of sequence in fragments shorter than    4245 bp
  70% of sequence in fragments shorter than    4695 bp
  75% of sequence in fragments shorter than    5211 bp
  80% of sequence in fragments shorter than    5823 bp
  85% of sequence in fragments shorter than    6606 bp
  90% of sequence in fragments shorter than    7685 bp
  95% of sequence in fragments shorter than    9479 bp

# AflIII.NheI (ACRYGT+GCTAGC) double digest, 1772 thousand fragments (1485394+287384 sites), 1681 bp average fragment length
   5% of sequence in fragments shorter than     612 bp
  10% of sequence in fragments shorter than     930 bp
  15% of sequence in fragments shorter than    1206 bp
  20% of sequence in fragments shorter than    1477 bp
  25% of sequence in fragments shorter than    1725 bp
  30% of sequence in fragments shorter than    1970 bp
  35% of sequence in fragments shorter than    2223 bp
  40% of sequence in fragments shorter than    2493 bp
  45% of sequence in fragments shorter than    2757 bp
  50% of sequence in fragments shorter than    3040 bp
  55% of sequence in fragments shorter than    3357 bp
  60% of sequence in fragments shorter than    3705 bp
  65% of sequence in fragments shorter than    4093 bp
  70% of sequence in fragments shorter than    4530 bp
  75% of sequence in fragments shorter than    5032 bp
  80% of sequence in fragments shorter than    5643 bp
  85% of sequence in fragments shorter than    6437 bp
  90% of sequence in fragments shorter than    7566 bp
  95% of sequence in fragments shorter than    9640 bp

# HhaI.SgrAI (GCGC+CRCCGGYG) double digest, 1771 thousand fragments (1756502+15270 sites), 1682 bp average fragment length
   5% of sequence in fragments shorter than     568 bp
  10% of sequence in fragments shorter than    1001 bp
  15% of sequence in fragments shorter than    1432 bp
  20% of sequence in fragments shorter than    1871 bp
  25% of sequence in fragments shorter than    2338 bp
  30% of sequence in fragments shorter than    2828 bp
  35% of sequence in fragments shorter than    3356 bp
  40% of sequence in fragments shorter than    3920 bp
  45% of sequence in fragments shorter than    4531 bp
  50% of sequence in fragments shorter than    5207 bp
  55% of sequence in fragments shorter than    5951 bp
  60% of sequence in fragments shorter than    6784 bp
  65% of sequence in fragments shorter than    7738 bp
  70% of sequence in fragments shorter than    8834 bp
  75% of sequence in fragments shorter than   10167 bp
  80% of sequence in fragments shorter than   11796 bp
  85% of sequence in fragments shorter than   13895 bp
  90% of sequence in fragments shorter than   16939 bp
  95% of sequence in fragments shorter than   22209 bp

# HhaI.NruI (GCGC+TCGCGA) double digest, 1770 thousand fragments (1756502+14872 sites), 1682 bp average fragment length
   5% of sequence in fragments shorter than     566 bp
  10% of sequence in fragments shorter than     999 bp
  15% of sequence in fragments shorter than    1429 bp
  20% of sequence in fragments shorter than    1866 bp
  25% of sequence in fragments shorter than    2333 bp
  30% of sequence in fragments shorter than    2820 bp
  35% of sequence in fragments shorter than    3346 bp
  40% of sequence in fragments shorter than    3908 bp
  45% of sequence in fragments shorter than    4518 bp
  50% of sequence in fragments shorter than    5191 bp
  55% of sequence in fragments shorter than    5933 bp
  60% of sequence in fragments shorter than    6765 bp
  65% of sequence in fragments shorter than    7711 bp
  70% of sequence in fragments shorter than    8808 bp
  75% of sequence in fragments shorter than   10131 bp
  80% of sequence in fragments shorter than   11762 bp
  85% of sequence in fragments shorter than   13857 bp
  90% of sequence in fragments shorter than   16889 bp
  95% of sequence in fragments shorter than   22150 bp

# HhaI.PvuI (GCGC+CGATCG) double digest, 1770 thousand fragments (1756502+14186 sites), 1683 bp average fragment length
   5% of sequence in fragments shorter than     563 bp
  10% of sequence in fragments shorter than     997 bp
  15% of sequence in fragments shorter than    1428 bp
  20% of sequence in fragments shorter than    1865 bp
  25% of sequence in fragments shorter than    2331 bp
  30% of sequence in fragments shorter than    2819 bp
  35% of sequence in fragments shorter than    3345 bp
  40% of sequence in fragments shorter than    3906 bp
  45% of sequence in fragments shorter than    4515 bp
  50% of sequence in fragments shorter than    5187 bp
  55% of sequence in fragments shorter than    5930 bp
  60% of sequence in fragments shorter than    6762 bp
  65% of sequence in fragments shorter than    7710 bp
  70% of sequence in fragments shorter than    8806 bp
  75% of sequence in fragments shorter than   10127 bp
  80% of sequence in fragments shorter than   11750 bp
  85% of sequence in fragments shorter than   13844 bp
  90% of sequence in fragments shorter than   16884 bp
  95% of sequence in fragments shorter than   22123 bp

# FseI.HhaI (GGCCGGCC+GCGC) double digest, 1769 thousand fragments (13662+1756502 sites), 1683 bp average fragment length
   5% of sequence in fragments shorter than     566 bp
  10% of sequence in fragments shorter than     999 bp
  15% of sequence in fragments shorter than    1431 bp
  20% of sequence in fragments shorter than    1870 bp
  25% of sequence in fragments shorter than    2339 bp
  30% of sequence in fragments shorter than    2828 bp
  35% of sequence in fragments shorter than    3357 bp
  40% of sequence in fragments shorter than    3923 bp
  45% of sequence in fragments shorter than    4535 bp
  50% of sequence in fragments shorter than    5213 bp
  55% of sequence in fragments shorter than    5959 bp
  60% of sequence in fragments shorter than    6795 bp
  65% of sequence in fragments shorter than    7750 bp
  70% of sequence in fragments shorter than    8851 bp
  75% of sequence in fragments shorter than   10191 bp
  80% of sequence in fragments shorter than   11827 bp
  85% of sequence in fragments shorter than   13928 bp
  90% of sequence in fragments shorter than   16987 bp
  95% of sequence in fragments shorter than   22306 bp

# PsiI.XhoI (TTATAA+CTCGAG) double digest, 1768 thousand fragments (1647912+121308 sites), 1684 bp average fragment length
   5% of sequence in fragments shorter than     569 bp
  10% of sequence in fragments shorter than     885 bp
  15% of sequence in fragments shorter than    1157 bp
  20% of sequence in fragments shorter than    1422 bp
  25% of sequence in fragments shorter than    1686 bp
  30% of sequence in fragments shorter than    1953 bp
  35% of sequence in fragments shorter than    2232 bp
  40% of sequence in fragments shorter than    2527 bp
  45% of sequence in fragments shorter than    2840 bp
  50% of sequence in fragments shorter than    3159 bp
  55% of sequence in fragments shorter than    3525 bp
  60% of sequence in fragments shorter than    3931 bp
  65% of sequence in fragments shorter than    4393 bp
  70% of sequence in fragments shorter than    4939 bp
  75% of sequence in fragments shorter than    5599 bp
  80% of sequence in fragments shorter than    6446 bp
  85% of sequence in fragments shorter than    7603 bp
  90% of sequence in fragments shorter than    9413 bp
  95% of sequence in fragments shorter than   13510 bp

# EcoRV.PstI (GATATC+CTGCAG) double digest, 1767 thousand fragments (446473+1321469 sites), 1686 bp average fragment length
   5% of sequence in fragments shorter than     582 bp
  10% of sequence in fragments shorter than     881 bp
  15% of sequence in fragments shorter than    1152 bp
  20% of sequence in fragments shorter than    1409 bp
  25% of sequence in fragments shorter than    1665 bp
  30% of sequence in fragments shorter than    1909 bp
  35% of sequence in fragments shorter than    2167 bp
  40% of sequence in fragments shorter than    2436 bp
  45% of sequence in fragments shorter than    2724 bp
  50% of sequence in fragments shorter than    3021 bp
  55% of sequence in fragments shorter than    3346 bp
  60% of sequence in fragments shorter than    3701 bp
  65% of sequence in fragments shorter than    4094 bp
  70% of sequence in fragments shorter than    4536 bp
  75% of sequence in fragments shorter than    5049 bp
  80% of sequence in fragments shorter than    5669 bp
  85% of sequence in fragments shorter than    6469 bp
  90% of sequence in fragments shorter than    7595 bp
  95% of sequence in fragments shorter than    9567 bp

# HhaI.NotI (GCGC+GCGGCCGC) double digest, 1765 thousand fragments (1756502+9795 sites), 1687 bp average fragment length
   5% of sequence in fragments shorter than     570 bp
  10% of sequence in fragments shorter than    1005 bp
  15% of sequence in fragments shorter than    1438 bp
  20% of sequence in fragments shorter than    1879 bp
  25% of sequence in fragments shorter than    2349 bp
  30% of sequence in fragments shorter than    2840 bp
  35% of sequence in fragments shorter than    3371 bp
  40% of sequence in fragments shorter than    3938 bp
  45% of sequence in fragments shorter than    4550 bp
  50% of sequence in fragments shorter than    5228 bp
  55% of sequence in fragments shorter than    5977 bp
  60% of sequence in fragments shorter than    6814 bp
  65% of sequence in fragments shorter than    7771 bp
  70% of sequence in fragments shorter than    8876 bp
  75% of sequence in fragments shorter than   10215 bp
  80% of sequence in fragments shorter than   11858 bp
  85% of sequence in fragments shorter than   13966 bp
  90% of sequence in fragments shorter than   17020 bp
  95% of sequence in fragments shorter than   22356 bp

# HhaI.RsrII (GCGC+CGGWCCG) double digest, 1765 thousand fragments (1756502+9764 sites), 1687 bp average fragment length
   5% of sequence in fragments shorter than     568 bp
  10% of sequence in fragments shorter than    1003 bp
  15% of sequence in fragments shorter than    1434 bp
  20% of sequence in fragments shorter than    1874 bp
  25% of sequence in fragments shorter than    2343 bp
  30% of sequence in fragments shorter than    2833 bp
  35% of sequence in fragments shorter than    3363 bp
  40% of sequence in fragments shorter than    3929 bp
  45% of sequence in fragments shorter than    4540 bp
  50% of sequence in fragments shorter than    5217 bp
  55% of sequence in fragments shorter than    5963 bp
  60% of sequence in fragments shorter than    6799 bp
  65% of sequence in fragments shorter than    7754 bp
  70% of sequence in fragments shorter than    8854 bp
  75% of sequence in fragments shorter than   10191 bp
  80% of sequence in fragments shorter than   11822 bp
  85% of sequence in fragments shorter than   13932 bp
  90% of sequence in fragments shorter than   16995 bp
  95% of sequence in fragments shorter than   22294 bp

# BfiI.PspXI (ACTGGG+VCTCGAGB) double digest, 1765 thousand fragments (1706594+59193 sites), 1688 bp average fragment length
   5% of sequence in fragments shorter than     571 bp
  10% of sequence in fragments shorter than     885 bp
  15% of sequence in fragments shorter than    1154 bp
  20% of sequence in fragments shorter than    1418 bp
  25% of sequence in fragments shorter than    1678 bp
  30% of sequence in fragments shorter than    1934 bp
  35% of sequence in fragments shorter than    2187 bp
  40% of sequence in fragments shorter than    2457 bp
  45% of sequence in fragments shorter than    2750 bp
  50% of sequence in fragments shorter than    3030 bp
  55% of sequence in fragments shorter than    3364 bp
  60% of sequence in fragments shorter than    3734 bp
  65% of sequence in fragments shorter than    4148 bp
  70% of sequence in fragments shorter than    4613 bp
  75% of sequence in fragments shorter than    5148 bp
  80% of sequence in fragments shorter than    5806 bp
  85% of sequence in fragments shorter than    6675 bp
  90% of sequence in fragments shorter than    7958 bp
  95% of sequence in fragments shorter than   10463 bp

# AvaI.NcoI (CYCGRG+CCATGG) double digest, 1764 thousand fragments (1005714+759594 sites), 1688 bp average fragment length
   5% of sequence in fragments shorter than     554 bp
  10% of sequence in fragments shorter than     885 bp
  15% of sequence in fragments shorter than    1185 bp
  20% of sequence in fragments shorter than    1471 bp
  25% of sequence in fragments shorter than    1766 bp
  30% of sequence in fragments shorter than    2066 bp
  35% of sequence in fragments shorter than    2379 bp
  40% of sequence in fragments shorter than    2714 bp
  45% of sequence in fragments shorter than    3066 bp
  50% of sequence in fragments shorter than    3445 bp
  55% of sequence in fragments shorter than    3861 bp
  60% of sequence in fragments shorter than    4274 bp
  65% of sequence in fragments shorter than    4768 bp
  70% of sequence in fragments shorter than    5345 bp
  75% of sequence in fragments shorter than    6031 bp
  80% of sequence in fragments shorter than    6898 bp
  85% of sequence in fragments shorter than    8039 bp
  90% of sequence in fragments shorter than    9758 bp
  95% of sequence in fragments shorter than   13113 bp

# HindIII.NdeI (AAGCTT+CATATG) double digest, 1763 thousand fragments (860365+903360 sites), 1690 bp average fragment length
   5% of sequence in fragments shorter than     578 bp
  10% of sequence in fragments shorter than     881 bp
  15% of sequence in fragments shorter than    1141 bp
  20% of sequence in fragments shorter than    1379 bp
  25% of sequence in fragments shorter than    1626 bp
  30% of sequence in fragments shorter than    1881 bp
  35% of sequence in fragments shorter than    2110 bp
  40% of sequence in fragments shorter than    2375 bp
  45% of sequence in fragments shorter than    2654 bp
  50% of sequence in fragments shorter than    2946 bp
  55% of sequence in fragments shorter than    3267 bp
  60% of sequence in fragments shorter than    3624 bp
  65% of sequence in fragments shorter than    4027 bp
  70% of sequence in fragments shorter than    4488 bp
  75% of sequence in fragments shorter than    5031 bp
  80% of sequence in fragments shorter than    5701 bp
  85% of sequence in fragments shorter than    6613 bp
  90% of sequence in fragments shorter than    7985 bp
  95% of sequence in fragments shorter than   10737 bp

# EsaBC3I.PacI (TCGA+TTAATTAA) double digest, 1762 thousand fragments (1603346+159455 sites), 1690 bp average fragment length
   5% of sequence in fragments shorter than     583 bp
  10% of sequence in fragments shorter than     889 bp
  15% of sequence in fragments shorter than    1165 bp
  20% of sequence in fragments shorter than    1411 bp
  25% of sequence in fragments shorter than    1669 bp
  30% of sequence in fragments shorter than    1923 bp
  35% of sequence in fragments shorter than    2189 bp
  40% of sequence in fragments shorter than    2463 bp
  45% of sequence in fragments shorter than    2750 bp
  50% of sequence in fragments shorter than    3051 bp
  55% of sequence in fragments shorter than    3375 bp
  60% of sequence in fragments shorter than    3730 bp
  65% of sequence in fragments shorter than    4129 bp
  70% of sequence in fragments shorter than    4580 bp
  75% of sequence in fragments shorter than    5103 bp
  80% of sequence in fragments shorter than    5740 bp
  85% of sequence in fragments shorter than    6562 bp
  90% of sequence in fragments shorter than    7707 bp
  95% of sequence in fragments shorter than    9656 bp

# AscI.HhaI (GGCGCGCC+GCGC) double digest, 1760 thousand fragments (4751+1756502 sites), 1692 bp average fragment length
   5% of sequence in fragments shorter than     571 bp
  10% of sequence in fragments shorter than    1007 bp
  15% of sequence in fragments shorter than    1440 bp
  20% of sequence in fragments shorter than    1882 bp
  25% of sequence in fragments shorter than    2352 bp
  30% of sequence in fragments shorter than    2843 bp
  35% of sequence in fragments shorter than    3374 bp
  40% of sequence in fragments shorter than    3941 bp
  45% of sequence in fragments shorter than    4554 bp
  50% of sequence in fragments shorter than    5231 bp
  55% of sequence in fragments shorter than    5980 bp
  60% of sequence in fragments shorter than    6818 bp
  65% of sequence in fragments shorter than    7775 bp
  70% of sequence in fragments shorter than    8880 bp
  75% of sequence in fragments shorter than   10219 bp
  80% of sequence in fragments shorter than   11862 bp
  85% of sequence in fragments shorter than   13974 bp
  90% of sequence in fragments shorter than   17030 bp
  95% of sequence in fragments shorter than   22360 bp

# EsaBC3I.Hpy99I (TCGA+CGWCG) double digest, 1760 thousand fragments (1603346+157917 sites), 1692 bp average fragment length
   5% of sequence in fragments shorter than     578 bp
  10% of sequence in fragments shorter than     897 bp
  15% of sequence in fragments shorter than    1185 bp
  20% of sequence in fragments shorter than    1447 bp
  25% of sequence in fragments shorter than    1721 bp
  30% of sequence in fragments shorter than    1997 bp
  35% of sequence in fragments shorter than    2281 bp
  40% of sequence in fragments shorter than    2578 bp
  45% of sequence in fragments shorter than    2890 bp
  50% of sequence in fragments shorter than    3218 bp
  55% of sequence in fragments shorter than    3576 bp
  60% of sequence in fragments shorter than    3970 bp
  65% of sequence in fragments shorter than    4413 bp
  70% of sequence in fragments shorter than    4913 bp
  75% of sequence in fragments shorter than    5504 bp
  80% of sequence in fragments shorter than    6217 bp
  85% of sequence in fragments shorter than    7132 bp
  90% of sequence in fragments shorter than    8404 bp
  95% of sequence in fragments shorter than   10610 bp

# AgeI.BfiI (ACCGGT+ACTGGG) double digest, 1759 thousand fragments (53386+1706594 sites), 1693 bp average fragment length
   5% of sequence in fragments shorter than     575 bp
  10% of sequence in fragments shorter than     889 bp
  15% of sequence in fragments shorter than    1156 bp
  20% of sequence in fragments shorter than    1415 bp
  25% of sequence in fragments shorter than    1675 bp
  30% of sequence in fragments shorter than    1931 bp
  35% of sequence in fragments shorter than    2189 bp
  40% of sequence in fragments shorter than    2463 bp
  45% of sequence in fragments shorter than    2755 bp
  50% of sequence in fragments shorter than    3036 bp
  55% of sequence in fragments shorter than    3370 bp
  60% of sequence in fragments shorter than    3736 bp
  65% of sequence in fragments shorter than    4148 bp
  70% of sequence in fragments shorter than    4612 bp
  75% of sequence in fragments shorter than    5151 bp
  80% of sequence in fragments shorter than    5809 bp
  85% of sequence in fragments shorter than    6676 bp
  90% of sequence in fragments shorter than    7947 bp
  95% of sequence in fragments shorter than   10454 bp

# NdeI.XbaI (CATATG+TCTAGA) double digest, 1753 thousand fragments (903360+850998 sites), 1699 bp average fragment length
   5% of sequence in fragments shorter than     557 bp
  10% of sequence in fragments shorter than     858 bp
  15% of sequence in fragments shorter than    1128 bp
  20% of sequence in fragments shorter than    1388 bp
  25% of sequence in fragments shorter than    1651 bp
  30% of sequence in fragments shorter than    1909 bp
  35% of sequence in fragments shorter than    2181 bp
  40% of sequence in fragments shorter than    2462 bp
  45% of sequence in fragments shorter than    2760 bp
  50% of sequence in fragments shorter than    3073 bp
  55% of sequence in fragments shorter than    3415 bp
  60% of sequence in fragments shorter than    3784 bp
  65% of sequence in fragments shorter than    4201 bp
  70% of sequence in fragments shorter than    4668 bp
  75% of sequence in fragments shorter than    5227 bp
  80% of sequence in fragments shorter than    5919 bp
  85% of sequence in fragments shorter than    6829 bp
  90% of sequence in fragments shorter than    8167 bp
  95% of sequence in fragments shorter than   10781 bp

# BglII.BspHI (AGATCT+TCATGA) double digest, 1752 thousand fragments (774732+978290 sites), 1700 bp average fragment length
   5% of sequence in fragments shorter than     605 bp
  10% of sequence in fragments shorter than     909 bp
  15% of sequence in fragments shorter than    1177 bp
  20% of sequence in fragments shorter than    1417 bp
  25% of sequence in fragments shorter than    1657 bp
  30% of sequence in fragments shorter than    1899 bp
  35% of sequence in fragments shorter than    2126 bp
  40% of sequence in fragments shorter than    2382 bp
  45% of sequence in fragments shorter than    2649 bp
  50% of sequence in fragments shorter than    2934 bp
  55% of sequence in fragments shorter than    3241 bp
  60% of sequence in fragments shorter than    3575 bp
  65% of sequence in fragments shorter than    3949 bp
  70% of sequence in fragments shorter than    4378 bp
  75% of sequence in fragments shorter than    4881 bp
  80% of sequence in fragments shorter than    5495 bp
  85% of sequence in fragments shorter than    6307 bp
  90% of sequence in fragments shorter than    7516 bp
  95% of sequence in fragments shorter than    9949 bp

# EcoRI.NdeI (GAATTC+CATATG) double digest, 1750 thousand fragments (847401+903360 sites), 1702 bp average fragment length
   5% of sequence in fragments shorter than     573 bp
  10% of sequence in fragments shorter than     873 bp
  15% of sequence in fragments shorter than    1143 bp
  20% of sequence in fragments shorter than    1393 bp
  25% of sequence in fragments shorter than    1649 bp
  30% of sequence in fragments shorter than    1910 bp
  35% of sequence in fragments shorter than    2176 bp
  40% of sequence in fragments shorter than    2451 bp
  45% of sequence in fragments shorter than    2745 bp
  50% of sequence in fragments shorter than    3052 bp
  55% of sequence in fragments shorter than    3381 bp
  60% of sequence in fragments shorter than    3745 bp
  65% of sequence in fragments shorter than    4152 bp
  70% of sequence in fragments shorter than    4614 bp
  75% of sequence in fragments shorter than    5150 bp
  80% of sequence in fragments shorter than    5816 bp
  85% of sequence in fragments shorter than    6694 bp
  90% of sequence in fragments shorter than    7999 bp
  95% of sequence in fragments shorter than   10508 bp

# BfiI.PmeI (ACTGGG+GTTTAAAC) double digest, 1748 thousand fragments (1706594+42094 sites), 1704 bp average fragment length
   5% of sequence in fragments shorter than     579 bp
  10% of sequence in fragments shorter than     895 bp
  15% of sequence in fragments shorter than    1167 bp
  20% of sequence in fragments shorter than    1432 bp
  25% of sequence in fragments shorter than    1692 bp
  30% of sequence in fragments shorter than    1946 bp
  35% of sequence in fragments shorter than    2196 bp
  40% of sequence in fragments shorter than    2467 bp
  45% of sequence in fragments shorter than    2757 bp
  50% of sequence in fragments shorter than    3037 bp
  55% of sequence in fragments shorter than    3367 bp
  60% of sequence in fragments shorter than    3735 bp
  65% of sequence in fragments shorter than    4142 bp
  70% of sequence in fragments shorter than    4603 bp
  75% of sequence in fragments shorter than    5129 bp
  80% of sequence in fragments shorter than    5777 bp
  85% of sequence in fragments shorter than    6630 bp
  90% of sequence in fragments shorter than    7887 bp
  95% of sequence in fragments shorter than   10329 bp

# AvaI.BclI (CYCGRG+TGATCA) double digest, 1744 thousand fragments (1005714+738785 sites), 1708 bp average fragment length
   5% of sequence in fragments shorter than     584 bp
  10% of sequence in fragments shorter than     904 bp
  15% of sequence in fragments shorter than    1192 bp
  20% of sequence in fragments shorter than    1470 bp
  25% of sequence in fragments shorter than    1737 bp
  30% of sequence in fragments shorter than    2016 bp
  35% of sequence in fragments shorter than    2289 bp
  40% of sequence in fragments shorter than    2583 bp
  45% of sequence in fragments shorter than    2892 bp
  50% of sequence in fragments shorter than    3226 bp
  55% of sequence in fragments shorter than    3592 bp
  60% of sequence in fragments shorter than    3991 bp
  65% of sequence in fragments shorter than    4435 bp
  70% of sequence in fragments shorter than    4951 bp
  75% of sequence in fragments shorter than    5555 bp
  80% of sequence in fragments shorter than    6304 bp
  85% of sequence in fragments shorter than    7285 bp
  90% of sequence in fragments shorter than    8713 bp
  95% of sequence in fragments shorter than   11610 bp

# AccI.AflII (GTMKAC+CTTAAG) double digest, 1743 thousand fragments (1101063+643284 sites), 1708 bp average fragment length
   5% of sequence in fragments shorter than     601 bp
  10% of sequence in fragments shorter than     910 bp
  15% of sequence in fragments shorter than    1178 bp
  20% of sequence in fragments shorter than    1419 bp
  25% of sequence in fragments shorter than    1664 bp
  30% of sequence in fragments shorter than    1884 bp
  35% of sequence in fragments shorter than    2130 bp
  40% of sequence in fragments shorter than    2387 bp
  45% of sequence in fragments shorter than    2656 bp
  50% of sequence in fragments shorter than    2939 bp
  55% of sequence in fragments shorter than    3252 bp
  60% of sequence in fragments shorter than    3588 bp
  65% of sequence in fragments shorter than    3948 bp
  70% of sequence in fragments shorter than    4350 bp
  75% of sequence in fragments shorter than    4844 bp
  80% of sequence in fragments shorter than    5452 bp
  85% of sequence in fragments shorter than    6237 bp
  90% of sequence in fragments shorter than    7391 bp
  95% of sequence in fragments shorter than    9613 bp

# AvaI.FnuDII (CYCGRG+CGCG) double digest, 1739 thousand fragments (1005714+733940 sites), 1713 bp average fragment length
   5% of sequence in fragments shorter than     608 bp
  10% of sequence in fragments shorter than    1091 bp
  15% of sequence in fragments shorter than    1554 bp
  20% of sequence in fragments shorter than    2034 bp
  25% of sequence in fragments shorter than    2532 bp
  30% of sequence in fragments shorter than    3067 bp
  35% of sequence in fragments shorter than    3652 bp
  40% of sequence in fragments shorter than    4284 bp
  45% of sequence in fragments shorter than    4976 bp
  50% of sequence in fragments shorter than    5731 bp
  55% of sequence in fragments shorter than    6573 bp
  60% of sequence in fragments shorter than    7528 bp
  65% of sequence in fragments shorter than    8627 bp
  70% of sequence in fragments shorter than    9890 bp
  75% of sequence in fragments shorter than   11444 bp
  80% of sequence in fragments shorter than   13357 bp
  85% of sequence in fragments shorter than   15913 bp
  90% of sequence in fragments shorter than   19630 bp
  95% of sequence in fragments shorter than   27225 bp

# BfiI.SalI (ACTGGG+GTCGAC) double digest, 1737 thousand fragments (1706594+31396 sites), 1715 bp average fragment length
   5% of sequence in fragments shorter than     582 bp
  10% of sequence in fragments shorter than     900 bp
  15% of sequence in fragments shorter than    1175 bp
  20% of sequence in fragments shorter than    1443 bp
  25% of sequence in fragments shorter than    1705 bp
  30% of sequence in fragments shorter than    1963 bp
  35% of sequence in fragments shorter than    2214 bp
  40% of sequence in fragments shorter than    2491 bp
  45% of sequence in fragments shorter than    2779 bp
  50% of sequence in fragments shorter than    3071 bp
  55% of sequence in fragments shorter than    3407 bp
  60% of sequence in fragments shorter than    3781 bp
  65% of sequence in fragments shorter than    4195 bp
  70% of sequence in fragments shorter than    4666 bp
  75% of sequence in fragments shorter than    5202 bp
  80% of sequence in fragments shorter than    5864 bp
  85% of sequence in fragments shorter than    6742 bp
  90% of sequence in fragments shorter than    8035 bp
  95% of sequence in fragments shorter than   10552 bp

# BspHI.NcoI (TCATGA+CCATGG) double digest, 1737 thousand fragments (978290+759594 sites), 1715 bp average fragment length
   5% of sequence in fragments shorter than     604 bp
  10% of sequence in fragments shorter than     906 bp
  15% of sequence in fragments shorter than    1173 bp
  20% of sequence in fragments shorter than    1417 bp
  25% of sequence in fragments shorter than    1653 bp
  30% of sequence in fragments shorter than    1901 bp
  35% of sequence in fragments shorter than    2146 bp
  40% of sequence in fragments shorter than    2403 bp
  45% of sequence in fragments shorter than    2675 bp
  50% of sequence in fragments shorter than    2961 bp
  55% of sequence in fragments shorter than    3256 bp
  60% of sequence in fragments shorter than    3591 bp
  65% of sequence in fragments shorter than    3963 bp
  70% of sequence in fragments shorter than    4378 bp
  75% of sequence in fragments shorter than    4863 bp
  80% of sequence in fragments shorter than    5456 bp
  85% of sequence in fragments shorter than    6217 bp
  90% of sequence in fragments shorter than    7331 bp
  95% of sequence in fragments shorter than    9448 bp

# FauI.SexAI (CCCGC+ACCWGGT) double digest, 1737 thousand fragments (1439772+297900 sites), 1715 bp average fragment length
   5% of sequence in fragments shorter than     589 bp
  10% of sequence in fragments shorter than    1013 bp
  15% of sequence in fragments shorter than    1397 bp
  20% of sequence in fragments shorter than    1792 bp
  25% of sequence in fragments shorter than    2182 bp
  30% of sequence in fragments shorter than    2602 bp
  35% of sequence in fragments shorter than    3044 bp
  40% of sequence in fragments shorter than    3515 bp
  45% of sequence in fragments shorter than    4024 bp
  50% of sequence in fragments shorter than    4552 bp
  55% of sequence in fragments shorter than    5156 bp
  60% of sequence in fragments shorter than    5833 bp
  65% of sequence in fragments shorter than    6591 bp
  70% of sequence in fragments shorter than    7456 bp
  75% of sequence in fragments shorter than    8489 bp
  80% of sequence in fragments shorter than    9770 bp
  85% of sequence in fragments shorter than   11443 bp
  90% of sequence in fragments shorter than   13891 bp
  95% of sequence in fragments shorter than   18632 bp

# BspMI.SexAI (ACCTGC+ACCWGGT) double digest, 1735 thousand fragments (1438129+297900 sites), 1717 bp average fragment length
   5% of sequence in fragments shorter than     579 bp
  10% of sequence in fragments shorter than     889 bp
  15% of sequence in fragments shorter than    1178 bp
  20% of sequence in fragments shorter than    1445 bp
  25% of sequence in fragments shorter than    1720 bp
  30% of sequence in fragments shorter than    1986 bp
  35% of sequence in fragments shorter than    2254 bp
  40% of sequence in fragments shorter than    2549 bp
  45% of sequence in fragments shorter than    2850 bp
  50% of sequence in fragments shorter than    3181 bp
  55% of sequence in fragments shorter than    3489 bp
  60% of sequence in fragments shorter than    3876 bp
  65% of sequence in fragments shorter than    4309 bp
  70% of sequence in fragments shorter than    4799 bp
  75% of sequence in fragments shorter than    5358 bp
  80% of sequence in fragments shorter than    6057 bp
  85% of sequence in fragments shorter than    6944 bp
  90% of sequence in fragments shorter than    8200 bp
  95% of sequence in fragments shorter than   10418 bp

# EsaBC3I.SnaBI (TCGA+TACGTA) double digest, 1735 thousand fragments (1603346+132299 sites), 1717 bp average fragment length
   5% of sequence in fragments shorter than     588 bp
  10% of sequence in fragments shorter than     898 bp
  15% of sequence in fragments shorter than    1173 bp
  20% of sequence in fragments shorter than    1428 bp
  25% of sequence in fragments shorter than    1690 bp
  30% of sequence in fragments shorter than    1949 bp
  35% of sequence in fragments shorter than    2221 bp
  40% of sequence in fragments shorter than    2501 bp
  45% of sequence in fragments shorter than    2795 bp
  50% of sequence in fragments shorter than    3103 bp
  55% of sequence in fragments shorter than    3438 bp
  60% of sequence in fragments shorter than    3805 bp
  65% of sequence in fragments shorter than    4211 bp
  70% of sequence in fragments shorter than    4677 bp
  75% of sequence in fragments shorter than    5220 bp
  80% of sequence in fragments shorter than    5874 bp
  85% of sequence in fragments shorter than    6715 bp
  90% of sequence in fragments shorter than    7898 bp
  95% of sequence in fragments shorter than    9917 bp

# ClaI.PsiI (ATCGAT+TTATAA) double digest, 1733 thousand fragments (86477+1647912 sites), 1718 bp average fragment length
   5% of sequence in fragments shorter than     574 bp
  10% of sequence in fragments shorter than     895 bp
  15% of sequence in fragments shorter than    1179 bp
  20% of sequence in fragments shorter than    1452 bp
  25% of sequence in fragments shorter than    1727 bp
  30% of sequence in fragments shorter than    2000 bp
  35% of sequence in fragments shorter than    2292 bp
  40% of sequence in fragments shorter than    2602 bp
  45% of sequence in fragments shorter than    2925 bp
  50% of sequence in fragments shorter than    3274 bp
  55% of sequence in fragments shorter than    3670 bp
  60% of sequence in fragments shorter than    4110 bp
  65% of sequence in fragments shorter than    4624 bp
  70% of sequence in fragments shorter than    5243 bp
  75% of sequence in fragments shorter than    6012 bp
  80% of sequence in fragments shorter than    7027 bp
  85% of sequence in fragments shorter than    8463 bp
  90% of sequence in fragments shorter than   10954 bp
  95% of sequence in fragments shorter than   17386 bp

# AccI.HaeII (GTMKAC+RGCGCY) double digest, 1730 thousand fragments (1101063+630101 sites), 1721 bp average fragment length
   5% of sequence in fragments shorter than     575 bp
  10% of sequence in fragments shorter than     905 bp
  15% of sequence in fragments shorter than    1198 bp
  20% of sequence in fragments shorter than    1477 bp
  25% of sequence in fragments shorter than    1749 bp
  30% of sequence in fragments shorter than    2021 bp
  35% of sequence in fragments shorter than    2290 bp
  40% of sequence in fragments shorter than    2575 bp
  45% of sequence in fragments shorter than    2869 bp
  50% of sequence in fragments shorter than    3182 bp
  55% of sequence in fragments shorter than    3515 bp
  60% of sequence in fragments shorter than    3877 bp
  65% of sequence in fragments shorter than    4263 bp
  70% of sequence in fragments shorter than    4701 bp
  75% of sequence in fragments shorter than    5204 bp
  80% of sequence in fragments shorter than    5792 bp
  85% of sequence in fragments shorter than    6544 bp
  90% of sequence in fragments shorter than    7592 bp
  95% of sequence in fragments shorter than    9417 bp

# EsaBC3I.NaeI (TCGA+GCCGGC) double digest, 1728 thousand fragments (1603346+125692 sites), 1723 bp average fragment length
   5% of sequence in fragments shorter than     581 bp
  10% of sequence in fragments shorter than     901 bp
  15% of sequence in fragments shorter than    1189 bp
  20% of sequence in fragments shorter than    1452 bp
  25% of sequence in fragments shorter than    1725 bp
  30% of sequence in fragments shorter than    2001 bp
  35% of sequence in fragments shorter than    2286 bp
  40% of sequence in fragments shorter than    2585 bp
  45% of sequence in fragments shorter than    2897 bp
  50% of sequence in fragments shorter than    3226 bp
  55% of sequence in fragments shorter than    3585 bp
  60% of sequence in fragments shorter than    3980 bp
  65% of sequence in fragments shorter than    4425 bp
  70% of sequence in fragments shorter than    4930 bp
  75% of sequence in fragments shorter than    5528 bp
  80% of sequence in fragments shorter than    6245 bp
  85% of sequence in fragments shorter than    7167 bp
  90% of sequence in fragments shorter than    8453 bp
  95% of sequence in fragments shorter than   10692 bp

# AflII.PpuMI (CTTAAG+RGGWCCY) double digest, 1728 thousand fragments (643284+1085138 sites), 1724 bp average fragment length
   5% of sequence in fragments shorter than     583 bp
  10% of sequence in fragments shorter than     913 bp
  15% of sequence in fragments shorter than    1202 bp
  20% of sequence in fragments shorter than    1479 bp
  25% of sequence in fragments shorter than    1749 bp
  30% of sequence in fragments shorter than    2019 bp
  35% of sequence in fragments shorter than    2296 bp
  40% of sequence in fragments shorter than    2583 bp
  45% of sequence in fragments shorter than    2885 bp
  50% of sequence in fragments shorter than    3201 bp
  55% of sequence in fragments shorter than    3519 bp
  60% of sequence in fragments shorter than    3887 bp
  65% of sequence in fragments shorter than    4307 bp
  70% of sequence in fragments shorter than    4791 bp
  75% of sequence in fragments shorter than    5357 bp
  80% of sequence in fragments shorter than    6051 bp
  85% of sequence in fragments shorter than    6969 bp
  90% of sequence in fragments shorter than    8315 bp
  95% of sequence in fragments shorter than   11028 bp

# BfiI.MluI (ACTGGG+ACGCGT) double digest, 1727 thousand fragments (1706594+21415 sites), 1724 bp average fragment length
   5% of sequence in fragments shorter than     584 bp
  10% of sequence in fragments shorter than     904 bp
  15% of sequence in fragments shorter than    1181 bp
  20% of sequence in fragments shorter than    1451 bp
  25% of sequence in fragments shorter than    1715 bp
  30% of sequence in fragments shorter than    1975 bp
  35% of sequence in fragments shorter than    2226 bp
  40% of sequence in fragments shorter than    2506 bp
  45% of sequence in fragments shorter than    2786 bp
  50% of sequence in fragments shorter than    3091 bp
  55% of sequence in fragments shorter than    3431 bp
  60% of sequence in fragments shorter than    3806 bp
  65% of sequence in fragments shorter than    4226 bp
  70% of sequence in fragments shorter than    4701 bp
  75% of sequence in fragments shorter than    5240 bp
  80% of sequence in fragments shorter than    5913 bp
  85% of sequence in fragments shorter than    6794 bp
  90% of sequence in fragments shorter than    8105 bp
  95% of sequence in fragments shorter than   10649 bp

# FauI.KpnI (CCCGC+GGTACC) double digest, 1727 thousand fragments (1439772+287974 sites), 1725 bp average fragment length
   5% of sequence in fragments shorter than     603 bp
  10% of sequence in fragments shorter than    1040 bp
  15% of sequence in fragments shorter than    1406 bp
  20% of sequence in fragments shorter than    1779 bp
  25% of sequence in fragments shorter than    2173 bp
  30% of sequence in fragments shorter than    2593 bp
  35% of sequence in fragments shorter than    3025 bp
  40% of sequence in fragments shorter than    3477 bp
  45% of sequence in fragments shorter than    3978 bp
  50% of sequence in fragments shorter than    4516 bp
  55% of sequence in fragments shorter than    5109 bp
  60% of sequence in fragments shorter than    5770 bp
  65% of sequence in fragments shorter than    6520 bp
  70% of sequence in fragments shorter than    7381 bp
  75% of sequence in fragments shorter than    8389 bp
  80% of sequence in fragments shorter than    9642 bp
  85% of sequence in fragments shorter than   11266 bp
  90% of sequence in fragments shorter than   13663 bp
  95% of sequence in fragments shorter than   18242 bp

# AflII.PvuII (CTTAAG+CAGCTG) double digest, 1727 thousand fragments (643284+1084260 sites), 1725 bp average fragment length
   5% of sequence in fragments shorter than     597 bp
  10% of sequence in fragments shorter than     910 bp
  15% of sequence in fragments shorter than    1185 bp
  20% of sequence in fragments shorter than    1444 bp
  25% of sequence in fragments shorter than    1701 bp
  30% of sequence in fragments shorter than    1957 bp
  35% of sequence in fragments shorter than    2217 bp
  40% of sequence in fragments shorter than    2484 bp
  45% of sequence in fragments shorter than    2756 bp
  50% of sequence in fragments shorter than    3051 bp
  55% of sequence in fragments shorter than    3376 bp
  60% of sequence in fragments shorter than    3702 bp
  65% of sequence in fragments shorter than    4093 bp
  70% of sequence in fragments shorter than    4542 bp
  75% of sequence in fragments shorter than    5064 bp
  80% of sequence in fragments shorter than    5708 bp
  85% of sequence in fragments shorter than    6536 bp
  90% of sequence in fragments shorter than    7742 bp
  95% of sequence in fragments shorter than   10143 bp

# ApaLI.BscGI (GTGCAC+CCCGT) double digest, 1726 thousand fragments (496255+1231070 sites), 1725 bp average fragment length
   5% of sequence in fragments shorter than     518 bp
  10% of sequence in fragments shorter than     864 bp
  15% of sequence in fragments shorter than    1191 bp
  20% of sequence in fragments shorter than    1508 bp
  25% of sequence in fragments shorter than    1828 bp
  30% of sequence in fragments shorter than    2155 bp
  35% of sequence in fragments shorter than    2495 bp
  40% of sequence in fragments shorter than    2851 bp
  45% of sequence in fragments shorter than    3232 bp
  50% of sequence in fragments shorter than    3633 bp
  55% of sequence in fragments shorter than    4074 bp
  60% of sequence in fragments shorter than    4560 bp
  65% of sequence in fragments shorter than    5068 bp
  70% of sequence in fragments shorter than    5682 bp
  75% of sequence in fragments shorter than    6396 bp
  80% of sequence in fragments shorter than    7272 bp
  85% of sequence in fragments shorter than    8429 bp
  90% of sequence in fragments shorter than   10023 bp
  95% of sequence in fragments shorter than   12764 bp

# FauI.NheI (CCCGC+GCTAGC) double digest, 1726 thousand fragments (1439772+287384 sites), 1725 bp average fragment length
   5% of sequence in fragments shorter than     606 bp
  10% of sequence in fragments shorter than    1042 bp
  15% of sequence in fragments shorter than    1436 bp
  20% of sequence in fragments shorter than    1805 bp
  25% of sequence in fragments shorter than    2186 bp
  30% of sequence in fragments shorter than    2593 bp
  35% of sequence in fragments shorter than    3000 bp
  40% of sequence in fragments shorter than    3455 bp
  45% of sequence in fragments shorter than    3951 bp
  50% of sequence in fragments shorter than    4486 bp
  55% of sequence in fragments shorter than    5069 bp
  60% of sequence in fragments shorter than    5722 bp
  65% of sequence in fragments shorter than    6460 bp
  70% of sequence in fragments shorter than    7314 bp
  75% of sequence in fragments shorter than    8313 bp
  80% of sequence in fragments shorter than    9550 bp
  85% of sequence in fragments shorter than   11151 bp
  90% of sequence in fragments shorter than   13446 bp
  95% of sequence in fragments shorter than   17790 bp

# BspMI.KpnI (ACCTGC+GGTACC) double digest, 1725 thousand fragments (1438129+287974 sites), 1726 bp average fragment length
   5% of sequence in fragments shorter than     600 bp
  10% of sequence in fragments shorter than     894 bp
  15% of sequence in fragments shorter than    1183 bp
  20% of sequence in fragments shorter than    1410 bp
  25% of sequence in fragments shorter than    1689 bp
  30% of sequence in fragments shorter than    1963 bp
  35% of sequence in fragments shorter than    2237 bp
  40% of sequence in fragments shorter than    2535 bp
  45% of sequence in fragments shorter than    2830 bp
  50% of sequence in fragments shorter than    3162 bp
  55% of sequence in fragments shorter than    3519 bp
  60% of sequence in fragments shorter than    3915 bp
  65% of sequence in fragments shorter than    4354 bp
  70% of sequence in fragments shorter than    4849 bp
  75% of sequence in fragments shorter than    5436 bp
  80% of sequence in fragments shorter than    6139 bp
  85% of sequence in fragments shorter than    7042 bp
  90% of sequence in fragments shorter than    8318 bp
  95% of sequence in fragments shorter than   10538 bp

# BspMI.NheI (ACCTGC+GCTAGC) double digest, 1725 thousand fragments (1438129+287384 sites), 1727 bp average fragment length
   5% of sequence in fragments shorter than     583 bp
  10% of sequence in fragments shorter than     910 bp
  15% of sequence in fragments shorter than    1188 bp
  20% of sequence in fragments shorter than    1445 bp
  25% of sequence in fragments shorter than    1713 bp
  30% of sequence in fragments shorter than    1965 bp
  35% of sequence in fragments shorter than    2226 bp
  40% of sequence in fragments shorter than    2514 bp
  45% of sequence in fragments shorter than    2816 bp
  50% of sequence in fragments shorter than    3140 bp
  55% of sequence in fragments shorter than    3493 bp
  60% of sequence in fragments shorter than    3884 bp
  65% of sequence in fragments shorter than    4319 bp
  70% of sequence in fragments shorter than    4811 bp
  75% of sequence in fragments shorter than    5389 bp
  80% of sequence in fragments shorter than    6089 bp
  85% of sequence in fragments shorter than    6981 bp
  90% of sequence in fragments shorter than    8244 bp
  95% of sequence in fragments shorter than   10414 bp

# EsaBC3I.XhoI (TCGA+CTCGAG) double digest, 1724 thousand fragments (1603346+121308 sites), 1728 bp average fragment length
   5% of sequence in fragments shorter than     635 bp
  10% of sequence in fragments shorter than     972 bp
  15% of sequence in fragments shorter than    1272 bp
  20% of sequence in fragments shorter than    1551 bp
  25% of sequence in fragments shorter than    1834 bp
  30% of sequence in fragments shorter than    2123 bp
  35% of sequence in fragments shorter than    2421 bp
  40% of sequence in fragments shorter than    2730 bp
  45% of sequence in fragments shorter than    3051 bp
  50% of sequence in fragments shorter than    3392 bp
  55% of sequence in fragments shorter than    3765 bp
  60% of sequence in fragments shorter than    4172 bp
  65% of sequence in fragments shorter than    4630 bp
  70% of sequence in fragments shorter than    5147 bp
  75% of sequence in fragments shorter than    5757 bp
  80% of sequence in fragments shorter than    6494 bp
  85% of sequence in fragments shorter than    7438 bp
  90% of sequence in fragments shorter than    8737 bp
  95% of sequence in fragments shorter than   11017 bp

# AclI.HgiCI (AACGTT+GGYRCC) double digest, 1723 thousand fragments (172706+1550877 sites), 1729 bp average fragment length
   5% of sequence in fragments shorter than     573 bp
  10% of sequence in fragments shorter than     893 bp
  15% of sequence in fragments shorter than    1177 bp
  20% of sequence in fragments shorter than    1435 bp
  25% of sequence in fragments shorter than    1700 bp
  30% of sequence in fragments shorter than    1982 bp
  35% of sequence in fragments shorter than    2268 bp
  40% of sequence in fragments shorter than    2574 bp
  45% of sequence in fragments shorter than    2882 bp
  50% of sequence in fragments shorter than    3223 bp
  55% of sequence in fragments shorter than    3597 bp
  60% of sequence in fragments shorter than    4015 bp
  65% of sequence in fragments shorter than    4473 bp
  70% of sequence in fragments shorter than    5011 bp
  75% of sequence in fragments shorter than    5638 bp
  80% of sequence in fragments shorter than    6422 bp
  85% of sequence in fragments shorter than    7454 bp
  90% of sequence in fragments shorter than    8937 bp
  95% of sequence in fragments shorter than   11831 bp

# AclI.BsmI (AACGTT+GAATGC) double digest, 1721 thousand fragments (172706+1549350 sites), 1730 bp average fragment length
   5% of sequence in fragments shorter than     603 bp
  10% of sequence in fragments shorter than    1021 bp
  15% of sequence in fragments shorter than    1366 bp
  20% of sequence in fragments shorter than    1690 bp
  25% of sequence in fragments shorter than    2002 bp
  30% of sequence in fragments shorter than    2309 bp
  35% of sequence in fragments shorter than    2608 bp
  40% of sequence in fragments shorter than    2920 bp
  45% of sequence in fragments shorter than    3248 bp
  50% of sequence in fragments shorter than    3596 bp
  55% of sequence in fragments shorter than    3966 bp
  60% of sequence in fragments shorter than    4370 bp
  65% of sequence in fragments shorter than    4812 bp
  70% of sequence in fragments shorter than    5315 bp
  75% of sequence in fragments shorter than    5895 bp
  80% of sequence in fragments shorter than    6588 bp
  85% of sequence in fragments shorter than    7455 bp
  90% of sequence in fragments shorter than    8668 bp
  95% of sequence in fragments shorter than   10699 bp

# BfiI.SgrAI (ACTGGG+CRCCGGYG) double digest, 1721 thousand fragments (1706594+15270 sites), 1731 bp average fragment length
   5% of sequence in fragments shorter than     586 bp
  10% of sequence in fragments shorter than     908 bp
  15% of sequence in fragments shorter than    1186 bp
  20% of sequence in fragments shorter than    1457 bp
  25% of sequence in fragments shorter than    1722 bp
  30% of sequence in fragments shorter than    1984 bp
  35% of sequence in fragments shorter than    2236 bp
  40% of sequence in fragments shorter than    2518 bp
  45% of sequence in fragments shorter than    2790 bp
  50% of sequence in fragments shorter than    3105 bp
  55% of sequence in fragments shorter than    3447 bp
  60% of sequence in fragments shorter than    3825 bp
  65% of sequence in fragments shorter than    4245 bp
  70% of sequence in fragments shorter than    4723 bp
  75% of sequence in fragments shorter than    5265 bp
  80% of sequence in fragments shorter than    5940 bp
  85% of sequence in fragments shorter than    6828 bp
  90% of sequence in fragments shorter than    8147 bp
  95% of sequence in fragments shorter than   10711 bp

# PstI.SpeI (CTGCAG+ACTAGT) double digest, 1721 thousand fragments (1321469+400286 sites), 1731 bp average fragment length
   5% of sequence in fragments shorter than     596 bp
  10% of sequence in fragments shorter than     903 bp
  15% of sequence in fragments shorter than    1183 bp
  20% of sequence in fragments shorter than    1447 bp
  25% of sequence in fragments shorter than    1712 bp
  30% of sequence in fragments shorter than    1973 bp
  35% of sequence in fragments shorter than    2237 bp
  40% of sequence in fragments shorter than    2515 bp
  45% of sequence in fragments shorter than    2808 bp
  50% of sequence in fragments shorter than    3117 bp
  55% of sequence in fragments shorter than    3453 bp
  60% of sequence in fragments shorter than    3818 bp
  65% of sequence in fragments shorter than    4183 bp
  70% of sequence in fragments shorter than    4646 bp
  75% of sequence in fragments shorter than    5166 bp
  80% of sequence in fragments shorter than    5812 bp
  85% of sequence in fragments shorter than    6643 bp
  90% of sequence in fragments shorter than    7813 bp
  95% of sequence in fragments shorter than    9981 bp

# BfiI.NruI (ACTGGG+TCGCGA) double digest, 1721 thousand fragments (1706594+14872 sites), 1731 bp average fragment length
   5% of sequence in fragments shorter than     587 bp
  10% of sequence in fragments shorter than     908 bp
  15% of sequence in fragments shorter than    1186 bp
  20% of sequence in fragments shorter than    1456 bp
  25% of sequence in fragments shorter than    1721 bp
  30% of sequence in fragments shorter than    1982 bp
  35% of sequence in fragments shorter than    2233 bp
  40% of sequence in fragments shorter than    2515 bp
  45% of sequence in fragments shorter than    2788 bp
  50% of sequence in fragments shorter than    3100 bp
  55% of sequence in fragments shorter than    3440 bp
  60% of sequence in fragments shorter than    3816 bp
  65% of sequence in fragments shorter than    4235 bp
  70% of sequence in fragments shorter than    4711 bp
  75% of sequence in fragments shorter than    5251 bp
  80% of sequence in fragments shorter than    5924 bp
  85% of sequence in fragments shorter than    6805 bp
  90% of sequence in fragments shorter than    8117 bp
  95% of sequence in fragments shorter than   10667 bp

# AflIII.BsrBI (ACRYGT+CCGCTC) double digest, 1720 thousand fragments (1485394+235523 sites), 1732 bp average fragment length
   5% of sequence in fragments shorter than     619 bp
  10% of sequence in fragments shorter than     967 bp
  15% of sequence in fragments shorter than    1261 bp
  20% of sequence in fragments shorter than    1542 bp
  25% of sequence in fragments shorter than    1813 bp
  30% of sequence in fragments shorter than    2080 bp
  35% of sequence in fragments shorter than    2348 bp
  40% of sequence in fragments shorter than    2632 bp
  45% of sequence in fragments shorter than    2914 bp
  50% of sequence in fragments shorter than    3223 bp
  55% of sequence in fragments shorter than    3557 bp
  60% of sequence in fragments shorter than    3919 bp
  65% of sequence in fragments shorter than    4326 bp
  70% of sequence in fragments shorter than    4783 bp
  75% of sequence in fragments shorter than    5315 bp
  80% of sequence in fragments shorter than    5957 bp
  85% of sequence in fragments shorter than    6755 bp
  90% of sequence in fragments shorter than    7898 bp
  95% of sequence in fragments shorter than    9972 bp

# BfiI.PvuI (ACTGGG+CGATCG) double digest, 1720 thousand fragments (1706594+14186 sites), 1732 bp average fragment length
   5% of sequence in fragments shorter than     587 bp
  10% of sequence in fragments shorter than     909 bp
  15% of sequence in fragments shorter than    1187 bp
  20% of sequence in fragments shorter than    1458 bp
  25% of sequence in fragments shorter than    1723 bp
  30% of sequence in fragments shorter than    1981 bp
  35% of sequence in fragments shorter than    2233 bp
  40% of sequence in fragments shorter than    2515 bp
  45% of sequence in fragments shorter than    2788 bp
  50% of sequence in fragments shorter than    3100 bp
  55% of sequence in fragments shorter than    3441 bp
  60% of sequence in fragments shorter than    3816 bp
  65% of sequence in fragments shorter than    4235 bp
  70% of sequence in fragments shorter than    4709 bp
  75% of sequence in fragments shorter than    5249 bp
  80% of sequence in fragments shorter than    5921 bp
  85% of sequence in fragments shorter than    6799 bp
  90% of sequence in fragments shorter than    8102 bp
  95% of sequence in fragments shorter than   10607 bp

# BfiI.FseI (ACTGGG+GGCCGGCC) double digest, 1719 thousand fragments (1706594+13662 sites), 1732 bp average fragment length
   5% of sequence in fragments shorter than     587 bp
  10% of sequence in fragments shorter than     909 bp
  15% of sequence in fragments shorter than    1187 bp
  20% of sequence in fragments shorter than    1458 bp
  25% of sequence in fragments shorter than    1723 bp
  30% of sequence in fragments shorter than    1986 bp
  35% of sequence in fragments shorter than    2238 bp
  40% of sequence in fragments shorter than    2521 bp
  45% of sequence in fragments shorter than    2792 bp
  50% of sequence in fragments shorter than    3109 bp
  55% of sequence in fragments shorter than    3451 bp
  60% of sequence in fragments shorter than    3830 bp
  65% of sequence in fragments shorter than    4251 bp
  70% of sequence in fragments shorter than    4729 bp
  75% of sequence in fragments shorter than    5271 bp
  80% of sequence in fragments shorter than    5947 bp
  85% of sequence in fragments shorter than    6835 bp
  90% of sequence in fragments shorter than    8156 bp
  95% of sequence in fragments shorter than   10727 bp

# AvaI.BsaAI (CYCGRG+YACGTR) double digest, 1719 thousand fragments (1005714+713801 sites), 1733 bp average fragment length
   5% of sequence in fragments shorter than     581 bp
  10% of sequence in fragments shorter than     922 bp
  15% of sequence in fragments shorter than    1224 bp
  20% of sequence in fragments shorter than    1525 bp
  25% of sequence in fragments shorter than    1830 bp
  30% of sequence in fragments shorter than    2135 bp
  35% of sequence in fragments shorter than    2460 bp
  40% of sequence in fragments shorter than    2802 bp
  45% of sequence in fragments shorter than    3164 bp
  50% of sequence in fragments shorter than    3537 bp
  55% of sequence in fragments shorter than    3966 bp
  60% of sequence in fragments shorter than    4423 bp
  65% of sequence in fragments shorter than    4955 bp
  70% of sequence in fragments shorter than    5562 bp
  75% of sequence in fragments shorter than    6283 bp
  80% of sequence in fragments shorter than    7174 bp
  85% of sequence in fragments shorter than    8332 bp
  90% of sequence in fragments shorter than   10011 bp
  95% of sequence in fragments shorter than   13111 bp

# AatII.PsiI (GACGTC+TTATAA) double digest, 1718 thousand fragments (71089+1647912 sites), 1733 bp average fragment length
   5% of sequence in fragments shorter than     582 bp
  10% of sequence in fragments shorter than     907 bp
  15% of sequence in fragments shorter than    1196 bp
  20% of sequence in fragments shorter than    1469 bp
  25% of sequence in fragments shorter than    1746 bp
  30% of sequence in fragments shorter than    2025 bp
  35% of sequence in fragments shorter than    2320 bp
  40% of sequence in fragments shorter than    2630 bp
  45% of sequence in fragments shorter than    2944 bp
  50% of sequence in fragments shorter than    3295 bp
  55% of sequence in fragments shorter than    3682 bp
  60% of sequence in fragments shorter than    4114 bp
  65% of sequence in fragments shorter than    4615 bp
  70% of sequence in fragments shorter than    5212 bp
  75% of sequence in fragments shorter than    5935 bp
  80% of sequence in fragments shorter than    6860 bp
  85% of sequence in fragments shorter than    8143 bp
  90% of sequence in fragments shorter than   10211 bp
  95% of sequence in fragments shorter than   15125 bp

# BclI.BspHI (TGATCA+TCATGA) double digest, 1716 thousand fragments (738785+978290 sites), 1735 bp average fragment length
   5% of sequence in fragments shorter than     612 bp
  10% of sequence in fragments shorter than     924 bp
  15% of sequence in fragments shorter than    1201 bp
  20% of sequence in fragments shorter than    1461 bp
  25% of sequence in fragments shorter than    1714 bp
  30% of sequence in fragments shorter than    1961 bp
  35% of sequence in fragments shorter than    2209 bp
  40% of sequence in fragments shorter than    2468 bp
  45% of sequence in fragments shorter than    2741 bp
  50% of sequence in fragments shorter than    3030 bp
  55% of sequence in fragments shorter than    3339 bp
  60% of sequence in fragments shorter than    3685 bp
  65% of sequence in fragments shorter than    4072 bp
  70% of sequence in fragments shorter than    4505 bp
  75% of sequence in fragments shorter than    5026 bp
  80% of sequence in fragments shorter than    5655 bp
  85% of sequence in fragments shorter than    6485 bp
  90% of sequence in fragments shorter than    7692 bp
  95% of sequence in fragments shorter than   10144 bp

# BfiI.NotI (ACTGGG+GCGGCCGC) double digest, 1715 thousand fragments (1706594+9795 sites), 1736 bp average fragment length
   5% of sequence in fragments shorter than     588 bp
  10% of sequence in fragments shorter than     910 bp
  15% of sequence in fragments shorter than    1189 bp
  20% of sequence in fragments shorter than    1461 bp
  25% of sequence in fragments shorter than    1724 bp
  30% of sequence in fragments shorter than    1989 bp
  35% of sequence in fragments shorter than    2241 bp
  40% of sequence in fragments shorter than    2525 bp
  45% of sequence in fragments shorter than    2795 bp
  50% of sequence in fragments shorter than    3113 bp
  55% of sequence in fragments shorter than    3456 bp
  60% of sequence in fragments shorter than    3834 bp
  65% of sequence in fragments shorter than    4256 bp
  70% of sequence in fragments shorter than    4734 bp
  75% of sequence in fragments shorter than    5277 bp
  80% of sequence in fragments shorter than    5951 bp
  85% of sequence in fragments shorter than    6841 bp
  90% of sequence in fragments shorter than    8163 bp
  95% of sequence in fragments shorter than   10733 bp

# BfiI.RsrII (ACTGGG+CGGWCCG) double digest, 1715 thousand fragments (1706594+9764 sites), 1736 bp average fragment length
   5% of sequence in fragments shorter than     588 bp
  10% of sequence in fragments shorter than     910 bp
  15% of sequence in fragments shorter than    1190 bp
  20% of sequence in fragments shorter than    1461 bp
  25% of sequence in fragments shorter than    1724 bp
  30% of sequence in fragments shorter than    1989 bp
  35% of sequence in fragments shorter than    2241 bp
  40% of sequence in fragments shorter than    2524 bp
  45% of sequence in fragments shorter than    2794 bp
  50% of sequence in fragments shorter than    3112 bp
  55% of sequence in fragments shorter than    3455 bp
  60% of sequence in fragments shorter than    3833 bp
  65% of sequence in fragments shorter than    4255 bp
  70% of sequence in fragments shorter than    4733 bp
  75% of sequence in fragments shorter than    5275 bp
  80% of sequence in fragments shorter than    5950 bp
  85% of sequence in fragments shorter than    6839 bp
  90% of sequence in fragments shorter than    8160 bp
  95% of sequence in fragments shorter than   10727 bp

# AflIII.KasI (ACRYGT+GGCGCC) double digest, 1715 thousand fragments (1485394+230381 sites), 1737 bp average fragment length
   5% of sequence in fragments shorter than     616 bp
  10% of sequence in fragments shorter than     962 bp
  15% of sequence in fragments shorter than    1258 bp
  20% of sequence in fragments shorter than    1537 bp
  25% of sequence in fragments shorter than    1806 bp
  30% of sequence in fragments shorter than    2071 bp
  35% of sequence in fragments shorter than    2335 bp
  40% of sequence in fragments shorter than    2615 bp
  45% of sequence in fragments shorter than    2901 bp
  50% of sequence in fragments shorter than    3204 bp
  55% of sequence in fragments shorter than    3535 bp
  60% of sequence in fragments shorter than    3896 bp
  65% of sequence in fragments shorter than    4299 bp
  70% of sequence in fragments shorter than    4751 bp
  75% of sequence in fragments shorter than    5258 bp
  80% of sequence in fragments shorter than    5875 bp
  85% of sequence in fragments shorter than    6664 bp
  90% of sequence in fragments shorter than    7795 bp
  95% of sequence in fragments shorter than    9872 bp

# HaeII.PpuMI (RGCGCY+RGGWCCY) double digest, 1714 thousand fragments (630101+1085138 sites), 1737 bp average fragment length
   5% of sequence in fragments shorter than     535 bp
  10% of sequence in fragments shorter than     886 bp
  15% of sequence in fragments shorter than    1228 bp
  20% of sequence in fragments shorter than    1572 bp
  25% of sequence in fragments shorter than    1921 bp
  30% of sequence in fragments shorter than    2266 bp
  35% of sequence in fragments shorter than    2630 bp
  40% of sequence in fragments shorter than    3025 bp
  45% of sequence in fragments shorter than    3420 bp
  50% of sequence in fragments shorter than    3833 bp
  55% of sequence in fragments shorter than    4308 bp
  60% of sequence in fragments shorter than    4839 bp
  65% of sequence in fragments shorter than    5437 bp
  70% of sequence in fragments shorter than    6115 bp
  75% of sequence in fragments shorter than    6914 bp
  80% of sequence in fragments shorter than    7893 bp
  85% of sequence in fragments shorter than    9119 bp
  90% of sequence in fragments shorter than   10885 bp
  95% of sequence in fragments shorter than   13875 bp

# PsiI.SacII (TTATAA+CCGCGG) double digest, 1714 thousand fragments (1647912+66901 sites), 1738 bp average fragment length
   5% of sequence in fragments shorter than     583 bp
  10% of sequence in fragments shorter than     913 bp
  15% of sequence in fragments shorter than    1206 bp
  20% of sequence in fragments shorter than    1484 bp
  25% of sequence in fragments shorter than    1766 bp
  30% of sequence in fragments shorter than    2048 bp
  35% of sequence in fragments shorter than    2346 bp
  40% of sequence in fragments shorter than    2660 bp
  45% of sequence in fragments shorter than    2975 bp
  50% of sequence in fragments shorter than    3329 bp
  55% of sequence in fragments shorter than    3719 bp
  60% of sequence in fragments shorter than    4152 bp
  65% of sequence in fragments shorter than    4658 bp
  70% of sequence in fragments shorter than    5260 bp
  75% of sequence in fragments shorter than    5991 bp
  80% of sequence in fragments shorter than    6915 bp
  85% of sequence in fragments shorter than    8197 bp
  90% of sequence in fragments shorter than   10240 bp
  95% of sequence in fragments shorter than   14915 bp

# HaeII.PvuII (RGCGCY+CAGCTG) double digest, 1713 thousand fragments (630101+1084260 sites), 1738 bp average fragment length
   5% of sequence in fragments shorter than     554 bp
  10% of sequence in fragments shorter than     883 bp
  15% of sequence in fragments shorter than    1206 bp
  20% of sequence in fragments shorter than    1527 bp
  25% of sequence in fragments shorter than    1850 bp
  30% of sequence in fragments shorter than    2175 bp
  35% of sequence in fragments shorter than    2520 bp
  40% of sequence in fragments shorter than    2882 bp
  45% of sequence in fragments shorter than    3268 bp
  50% of sequence in fragments shorter than    3682 bp
  55% of sequence in fragments shorter than    4140 bp
  60% of sequence in fragments shorter than    4643 bp
  65% of sequence in fragments shorter than    5214 bp
  70% of sequence in fragments shorter than    5854 bp
  75% of sequence in fragments shorter than    6603 bp
  80% of sequence in fragments shorter than    7493 bp
  85% of sequence in fragments shorter than    8649 bp
  90% of sequence in fragments shorter than   10345 bp
  95% of sequence in fragments shorter than   13255 bp

# BspHI.FnuDII (TCATGA+CGCG) double digest, 1711 thousand fragments (978290+733940 sites), 1740 bp average fragment length
   5% of sequence in fragments shorter than     669 bp
  10% of sequence in fragments shorter than    1063 bp
  15% of sequence in fragments shorter than    1406 bp
  20% of sequence in fragments shorter than    1728 bp
  25% of sequence in fragments shorter than    2028 bp
  30% of sequence in fragments shorter than    2320 bp
  35% of sequence in fragments shorter than    2629 bp
  40% of sequence in fragments shorter than    2951 bp
  45% of sequence in fragments shorter than    3279 bp
  50% of sequence in fragments shorter than    3630 bp
  55% of sequence in fragments shorter than    4008 bp
  60% of sequence in fragments shorter than    4405 bp
  65% of sequence in fragments shorter than    4862 bp
  70% of sequence in fragments shorter than    5378 bp
  75% of sequence in fragments shorter than    5979 bp
  80% of sequence in fragments shorter than    6704 bp
  85% of sequence in fragments shorter than    7646 bp
  90% of sequence in fragments shorter than    9015 bp
  95% of sequence in fragments shorter than   11569 bp

# AflIII.SwaI (ACRYGT+ATTTAAAT) double digest, 1711 thousand fragments (1485394+226602 sites), 1741 bp average fragment length
   5% of sequence in fragments shorter than     621 bp
  10% of sequence in fragments shorter than     963 bp
  15% of sequence in fragments shorter than    1259 bp
  20% of sequence in fragments shorter than    1540 bp
  25% of sequence in fragments shorter than    1814 bp
  30% of sequence in fragments shorter than    2083 bp
  35% of sequence in fragments shorter than    2352 bp
  40% of sequence in fragments shorter than    2638 bp
  45% of sequence in fragments shorter than    2931 bp
  50% of sequence in fragments shorter than    3244 bp
  55% of sequence in fragments shorter than    3586 bp
  60% of sequence in fragments shorter than    3960 bp
  65% of sequence in fragments shorter than    4379 bp
  70% of sequence in fragments shorter than    4863 bp
  75% of sequence in fragments shorter than    5428 bp
  80% of sequence in fragments shorter than    6089 bp
  85% of sequence in fragments shorter than    6933 bp
  90% of sequence in fragments shorter than    8171 bp
  95% of sequence in fragments shorter than   10505 bp

# AscI.BfiI (GGCGCGCC+ACTGGG) double digest, 1710 thousand fragments (4751+1706594 sites), 1741 bp average fragment length
   5% of sequence in fragments shorter than     590 bp
  10% of sequence in fragments shorter than     913 bp
  15% of sequence in fragments shorter than    1193 bp
  20% of sequence in fragments shorter than    1465 bp
  25% of sequence in fragments shorter than    1727 bp
  30% of sequence in fragments shorter than    1995 bp
  35% of sequence in fragments shorter than    2247 bp
  40% of sequence in fragments shorter than    2530 bp
  45% of sequence in fragments shorter than    2800 bp
  50% of sequence in fragments shorter than    3119 bp
  55% of sequence in fragments shorter than    3463 bp
  60% of sequence in fragments shorter than    3841 bp
  65% of sequence in fragments shorter than    4264 bp
  70% of sequence in fragments shorter than    4743 bp
  75% of sequence in fragments shorter than    5286 bp
  80% of sequence in fragments shorter than    5961 bp
  85% of sequence in fragments shorter than    6851 bp
  90% of sequence in fragments shorter than    8171 bp
  95% of sequence in fragments shorter than   10746 bp

# HindIII.XbaI (AAGCTT+TCTAGA) double digest, 1710 thousand fragments (860365+850998 sites), 1741 bp average fragment length
   5% of sequence in fragments shorter than     598 bp
  10% of sequence in fragments shorter than     914 bp
  15% of sequence in fragments shorter than    1191 bp
  20% of sequence in fragments shorter than    1438 bp
  25% of sequence in fragments shorter than    1676 bp
  30% of sequence in fragments shorter than    1918 bp
  35% of sequence in fragments shorter than    2176 bp
  40% of sequence in fragments shorter than    2443 bp
  45% of sequence in fragments shorter than    2724 bp
  50% of sequence in fragments shorter than    3020 bp
  55% of sequence in fragments shorter than    3341 bp
  60% of sequence in fragments shorter than    3694 bp
  65% of sequence in fragments shorter than    4085 bp
  70% of sequence in fragments shorter than    4531 bp
  75% of sequence in fragments shorter than    5046 bp
  80% of sequence in fragments shorter than    5678 bp
  85% of sequence in fragments shorter than    6503 bp
  90% of sequence in fragments shorter than    7705 bp
  95% of sequence in fragments shorter than    9936 bp

# HpaI.PstI (GTTAAC+CTGCAG) double digest, 1710 thousand fragments (389418+1321469 sites), 1742 bp average fragment length
   5% of sequence in fragments shorter than     597 bp
  10% of sequence in fragments shorter than     909 bp
  15% of sequence in fragments shorter than    1188 bp
  20% of sequence in fragments shorter than    1452 bp
  25% of sequence in fragments shorter than    1720 bp
  30% of sequence in fragments shorter than    1986 bp
  35% of sequence in fragments shorter than    2254 bp
  40% of sequence in fragments shorter than    2544 bp
  45% of sequence in fragments shorter than    2845 bp
  50% of sequence in fragments shorter than    3168 bp
  55% of sequence in fragments shorter than    3518 bp
  60% of sequence in fragments shorter than    3899 bp
  65% of sequence in fragments shorter than    4325 bp
  70% of sequence in fragments shorter than    4805 bp
  75% of sequence in fragments shorter than    5359 bp
  80% of sequence in fragments shorter than    6026 bp
  85% of sequence in fragments shorter than    6889 bp
  90% of sequence in fragments shorter than    8134 bp
  95% of sequence in fragments shorter than   10439 bp

# AvaI.BceAI (CYCGRG+ACGGC) double digest, 1710 thousand fragments (1005714+705178 sites), 1742 bp average fragment length
   5% of sequence in fragments shorter than     554 bp
  10% of sequence in fragments shorter than     925 bp
  15% of sequence in fragments shorter than    1275 bp
  20% of sequence in fragments shorter than    1627 bp
  25% of sequence in fragments shorter than    1990 bp
  30% of sequence in fragments shorter than    2373 bp
  35% of sequence in fragments shorter than    2782 bp
  40% of sequence in fragments shorter than    3213 bp
  45% of sequence in fragments shorter than    3689 bp
  50% of sequence in fragments shorter than    4208 bp
  55% of sequence in fragments shorter than    4786 bp
  60% of sequence in fragments shorter than    5426 bp
  65% of sequence in fragments shorter than    6153 bp
  70% of sequence in fragments shorter than    6993 bp
  75% of sequence in fragments shorter than    7990 bp
  80% of sequence in fragments shorter than    9232 bp
  85% of sequence in fragments shorter than   10871 bp
  90% of sequence in fragments shorter than   13289 bp
  95% of sequence in fragments shorter than   17861 bp

# HgiCI.PacI (GGYRCC+TTAATTAA) double digest, 1709 thousand fragments (1550877+159455 sites), 1742 bp average fragment length
   5% of sequence in fragments shorter than     584 bp
  10% of sequence in fragments shorter than     908 bp
  15% of sequence in fragments shorter than    1198 bp
  20% of sequence in fragments shorter than    1457 bp
  25% of sequence in fragments shorter than    1720 bp
  30% of sequence in fragments shorter than    2003 bp
  35% of sequence in fragments shorter than    2294 bp
  40% of sequence in fragments shorter than    2597 bp
  45% of sequence in fragments shorter than    2901 bp
  50% of sequence in fragments shorter than    3237 bp
  55% of sequence in fragments shorter than    3608 bp
  60% of sequence in fragments shorter than    4021 bp
  65% of sequence in fragments shorter than    4477 bp
  70% of sequence in fragments shorter than    5010 bp
  75% of sequence in fragments shorter than    5637 bp
  80% of sequence in fragments shorter than    6413 bp
  85% of sequence in fragments shorter than    7440 bp
  90% of sequence in fragments shorter than    8973 bp
  95% of sequence in fragments shorter than   12117 bp

# HgiCI.Hpy99I (GGYRCC+CGWCG) double digest, 1708 thousand fragments (1550877+157917 sites), 1744 bp average fragment length
   5% of sequence in fragments shorter than     572 bp
  10% of sequence in fragments shorter than     912 bp
  15% of sequence in fragments shorter than    1216 bp
  20% of sequence in fragments shorter than    1498 bp
  25% of sequence in fragments shorter than    1786 bp
  30% of sequence in fragments shorter than    2096 bp
  35% of sequence in fragments shorter than    2419 bp
  40% of sequence in fragments shorter than    2753 bp
  45% of sequence in fragments shorter than    3092 bp
  50% of sequence in fragments shorter than    3478 bp
  55% of sequence in fragments shorter than    3901 bp
  60% of sequence in fragments shorter than    4368 bp
  65% of sequence in fragments shorter than    4897 bp
  70% of sequence in fragments shorter than    5511 bp
  75% of sequence in fragments shorter than    6236 bp
  80% of sequence in fragments shorter than    7147 bp
  85% of sequence in fragments shorter than    8340 bp
  90% of sequence in fragments shorter than   10108 bp
  95% of sequence in fragments shorter than   13701 bp

# BsmI.PacI (GAATGC+TTAATTAA) double digest, 1708 thousand fragments (1549350+159455 sites), 1744 bp average fragment length
   5% of sequence in fragments shorter than     609 bp
  10% of sequence in fragments shorter than    1038 bp
  15% of sequence in fragments shorter than    1389 bp
  20% of sequence in fragments shorter than    1716 bp
  25% of sequence in fragments shorter than    2028 bp
  30% of sequence in fragments shorter than    2336 bp
  35% of sequence in fragments shorter than    2639 bp
  40% of sequence in fragments shorter than    2951 bp
  45% of sequence in fragments shorter than    3280 bp
  50% of sequence in fragments shorter than    3631 bp
  55% of sequence in fragments shorter than    4004 bp
  60% of sequence in fragments shorter than    4412 bp
  65% of sequence in fragments shorter than    4859 bp
  70% of sequence in fragments shorter than    5366 bp
  75% of sequence in fragments shorter than    5948 bp
  80% of sequence in fragments shorter than    6650 bp
  85% of sequence in fragments shorter than    7529 bp
  90% of sequence in fragments shorter than    8751 bp
  95% of sequence in fragments shorter than   10830 bp

# AvaIII.BglII (ATGCAT+AGATCT) double digest, 1707 thousand fragments (933357+774732 sites), 1745 bp average fragment length
   5% of sequence in fragments shorter than     611 bp
  10% of sequence in fragments shorter than     922 bp
  15% of sequence in fragments shorter than    1196 bp
  20% of sequence in fragments shorter than    1452 bp
  25% of sequence in fragments shorter than    1695 bp
  30% of sequence in fragments shorter than    1950 bp
  35% of sequence in fragments shorter than    2200 bp
  40% of sequence in fragments shorter than    2466 bp
  45% of sequence in fragments shorter than    2744 bp
  50% of sequence in fragments shorter than    3041 bp
  55% of sequence in fragments shorter than    3362 bp
  60% of sequence in fragments shorter than    3711 bp
  65% of sequence in fragments shorter than    4103 bp
  70% of sequence in fragments shorter than    4548 bp
  75% of sequence in fragments shorter than    5067 bp
  80% of sequence in fragments shorter than    5707 bp
  85% of sequence in fragments shorter than    6552 bp
  90% of sequence in fragments shorter than    7803 bp
  95% of sequence in fragments shorter than   10305 bp

# EcoRI.HindIII (GAATTC+AAGCTT) double digest, 1707 thousand fragments (847401+860365 sites), 1745 bp average fragment length
   5% of sequence in fragments shorter than     588 bp
  10% of sequence in fragments shorter than     908 bp
  15% of sequence in fragments shorter than    1199 bp
  20% of sequence in fragments shorter than    1463 bp
  25% of sequence in fragments shorter than    1709 bp
  30% of sequence in fragments shorter than    1966 bp
  35% of sequence in fragments shorter than    2232 bp
  40% of sequence in fragments shorter than    2505 bp
  45% of sequence in fragments shorter than    2787 bp
  50% of sequence in fragments shorter than    3087 bp
  55% of sequence in fragments shorter than    3408 bp
  60% of sequence in fragments shorter than    3759 bp
  65% of sequence in fragments shorter than    4149 bp
  70% of sequence in fragments shorter than    4589 bp
  75% of sequence in fragments shorter than    5105 bp
  80% of sequence in fragments shorter than    5727 bp
  85% of sequence in fragments shorter than    6539 bp
  90% of sequence in fragments shorter than    7707 bp
  95% of sequence in fragments shorter than    9826 bp

# BsmI.Hpy99I (GAATGC+CGWCG) double digest, 1706 thousand fragments (1549350+157917 sites), 1745 bp average fragment length
   5% of sequence in fragments shorter than     603 bp
  10% of sequence in fragments shorter than    1038 bp
  15% of sequence in fragments shorter than    1394 bp
  20% of sequence in fragments shorter than    1727 bp
  25% of sequence in fragments shorter than    2049 bp
  30% of sequence in fragments shorter than    2363 bp
  35% of sequence in fragments shorter than    2674 bp
  40% of sequence in fragments shorter than    2991 bp
  45% of sequence in fragments shorter than    3328 bp
  50% of sequence in fragments shorter than    3686 bp
  55% of sequence in fragments shorter than    4066 bp
  60% of sequence in fragments shorter than    4478 bp
  65% of sequence in fragments shorter than    4933 bp
  70% of sequence in fragments shorter than    5449 bp
  75% of sequence in fragments shorter than    6035 bp
  80% of sequence in fragments shorter than    6737 bp
  85% of sequence in fragments shorter than    7616 bp
  90% of sequence in fragments shorter than    8847 bp
  95% of sequence in fragments shorter than   10872 bp

# PsiI.PspXI (TTATAA+VCTCGAGB) double digest, 1706 thousand fragments (1647912+59193 sites), 1746 bp average fragment length
   5% of sequence in fragments shorter than     586 bp
  10% of sequence in fragments shorter than     913 bp
  15% of sequence in fragments shorter than    1203 bp
  20% of sequence in fragments shorter than    1479 bp
  25% of sequence in fragments shorter than    1759 bp
  30% of sequence in fragments shorter than    2040 bp
  35% of sequence in fragments shorter than    2336 bp
  40% of sequence in fragments shorter than    2649 bp
  45% of sequence in fragments shorter than    2963 bp
  50% of sequence in fragments shorter than    3317 bp
  55% of sequence in fragments shorter than    3704 bp
  60% of sequence in fragments shorter than    4139 bp
  65% of sequence in fragments shorter than    4648 bp
  70% of sequence in fragments shorter than    5248 bp
  75% of sequence in fragments shorter than    5977 bp
  80% of sequence in fragments shorter than    6912 bp
  85% of sequence in fragments shorter than    8210 bp
  90% of sequence in fragments shorter than   10335 bp
  95% of sequence in fragments shorter than   15254 bp

# AccI.SacI (GTMKAC+GAGCTC) double digest, 1703 thousand fragments (1101063+603323 sites), 1748 bp average fragment length
   5% of sequence in fragments shorter than     618 bp
  10% of sequence in fragments shorter than     934 bp
  15% of sequence in fragments shorter than    1207 bp
  20% of sequence in fragments shorter than    1449 bp
  25% of sequence in fragments shorter than    1697 bp
  30% of sequence in fragments shorter than    1936 bp
  35% of sequence in fragments shorter than    2182 bp
  40% of sequence in fragments shorter than    2438 bp
  45% of sequence in fragments shorter than    2703 bp
  50% of sequence in fragments shorter than    2981 bp
  55% of sequence in fragments shorter than    3283 bp
  60% of sequence in fragments shorter than    3609 bp
  65% of sequence in fragments shorter than    3974 bp
  70% of sequence in fragments shorter than    4339 bp
  75% of sequence in fragments shorter than    4809 bp
  80% of sequence in fragments shorter than    5371 bp
  85% of sequence in fragments shorter than    6076 bp
  90% of sequence in fragments shorter than    7088 bp
  95% of sequence in fragments shorter than    8905 bp

# AvaI.BciVI (CYCGRG+GTATCC) double digest, 1701 thousand fragments (1005714+696093 sites), 1751 bp average fragment length
   5% of sequence in fragments shorter than     600 bp
  10% of sequence in fragments shorter than     936 bp
  15% of sequence in fragments shorter than    1230 bp
  20% of sequence in fragments shorter than    1515 bp
  25% of sequence in fragments shorter than    1791 bp
  30% of sequence in fragments shorter than    2072 bp
  35% of sequence in fragments shorter than    2361 bp
  40% of sequence in fragments shorter than    2665 bp
  45% of sequence in fragments shorter than    2983 bp
  50% of sequence in fragments shorter than    3326 bp
  55% of sequence in fragments shorter than    3698 bp
  60% of sequence in fragments shorter than    4106 bp
  65% of sequence in fragments shorter than    4556 bp
  70% of sequence in fragments shorter than    5081 bp
  75% of sequence in fragments shorter than    5700 bp
  80% of sequence in fragments shorter than    6458 bp
  85% of sequence in fragments shorter than    7444 bp
  90% of sequence in fragments shorter than    8871 bp
  95% of sequence in fragments shorter than   11539 bp

# AgeI.PsiI (ACCGGT+TTATAA) double digest, 1700 thousand fragments (53386+1647912 sites), 1752 bp average fragment length
   5% of sequence in fragments shorter than     587 bp
  10% of sequence in fragments shorter than     910 bp
  15% of sequence in fragments shorter than    1203 bp
  20% of sequence in fragments shorter than    1481 bp
  25% of sequence in fragments shorter than    1763 bp
  30% of sequence in fragments shorter than    2046 bp
  35% of sequence in fragments shorter than    2344 bp
  40% of sequence in fragments shorter than    2660 bp
  45% of sequence in fragments shorter than    2980 bp
  50% of sequence in fragments shorter than    3342 bp
  55% of sequence in fragments shorter than    3737 bp
  60% of sequence in fragments shorter than    4184 bp
  65% of sequence in fragments shorter than    4707 bp
  70% of sequence in fragments shorter than    5329 bp
  75% of sequence in fragments shorter than    6098 bp
  80% of sequence in fragments shorter than    7085 bp
  85% of sequence in fragments shorter than    8492 bp
  90% of sequence in fragments shorter than   10875 bp
  95% of sequence in fragments shorter than   16722 bp

# BglII.StuI (AGATCT+AGGCCT) double digest, 1699 thousand fragments (774732+925729 sites), 1752 bp average fragment length
   5% of sequence in fragments shorter than     580 bp
  10% of sequence in fragments shorter than     889 bp
  15% of sequence in fragments shorter than    1189 bp
  20% of sequence in fragments shorter than    1462 bp
  25% of sequence in fragments shorter than    1739 bp
  30% of sequence in fragments shorter than    2021 bp
  35% of sequence in fragments shorter than    2312 bp
  40% of sequence in fragments shorter than    2608 bp
  45% of sequence in fragments shorter than    2916 bp
  50% of sequence in fragments shorter than    3244 bp
  55% of sequence in fragments shorter than    3595 bp
  60% of sequence in fragments shorter than    3977 bp
  65% of sequence in fragments shorter than    4391 bp
  70% of sequence in fragments shorter than    4861 bp
  75% of sequence in fragments shorter than    5400 bp
  80% of sequence in fragments shorter than    6052 bp
  85% of sequence in fragments shorter than    6881 bp
  90% of sequence in fragments shorter than    8022 bp
  95% of sequence in fragments shorter than    9970 bp

# PstI.SapI (CTGCAG+GCTCTTC) double digest, 1698 thousand fragments (1321469+377161 sites), 1754 bp average fragment length
   5% of sequence in fragments shorter than     594 bp
  10% of sequence in fragments shorter than     902 bp
  15% of sequence in fragments shorter than    1202 bp
  20% of sequence in fragments shorter than    1477 bp
  25% of sequence in fragments shorter than    1763 bp
  30% of sequence in fragments shorter than    2051 bp
  35% of sequence in fragments shorter than    2340 bp
  40% of sequence in fragments shorter than    2654 bp
  45% of sequence in fragments shorter than    2980 bp
  50% of sequence in fragments shorter than    3337 bp
  55% of sequence in fragments shorter than    3722 bp
  60% of sequence in fragments shorter than    4143 bp
  65% of sequence in fragments shorter than    4617 bp
  70% of sequence in fragments shorter than    5148 bp
  75% of sequence in fragments shorter than    5776 bp
  80% of sequence in fragments shorter than    6544 bp
  85% of sequence in fragments shorter than    7546 bp
  90% of sequence in fragments shorter than    8991 bp
  95% of sequence in fragments shorter than   11650 bp

# PstI.SmaI (CTGCAG+CCCGGG) double digest, 1697 thousand fragments (1321469+376940 sites), 1755 bp average fragment length
   5% of sequence in fragments shorter than     574 bp
  10% of sequence in fragments shorter than     898 bp
  15% of sequence in fragments shorter than    1201 bp
  20% of sequence in fragments shorter than    1492 bp
  25% of sequence in fragments shorter than    1796 bp
  30% of sequence in fragments shorter than    2097 bp
  35% of sequence in fragments shorter than    2423 bp
  40% of sequence in fragments shorter than    2766 bp
  45% of sequence in fragments shorter than    3134 bp
  50% of sequence in fragments shorter than    3532 bp
  55% of sequence in fragments shorter than    3975 bp
  60% of sequence in fragments shorter than    4470 bp
  65% of sequence in fragments shorter than    5017 bp
  70% of sequence in fragments shorter than    5639 bp
  75% of sequence in fragments shorter than    6370 bp
  80% of sequence in fragments shorter than    7275 bp
  85% of sequence in fragments shorter than    8433 bp
  90% of sequence in fragments shorter than   10126 bp
  95% of sequence in fragments shorter than   13356 bp

# EcoRI.XbaI (GAATTC+TCTAGA) double digest, 1697 thousand fragments (847401+850998 sites), 1755 bp average fragment length
   5% of sequence in fragments shorter than     607 bp
  10% of sequence in fragments shorter than     906 bp
  15% of sequence in fragments shorter than    1205 bp
  20% of sequence in fragments shorter than    1466 bp
  25% of sequence in fragments shorter than    1734 bp
  30% of sequence in fragments shorter than    1999 bp
  35% of sequence in fragments shorter than    2260 bp
  40% of sequence in fragments shorter than    2540 bp
  45% of sequence in fragments shorter than    2832 bp
  50% of sequence in fragments shorter than    3139 bp
  55% of sequence in fragments shorter than    3463 bp
  60% of sequence in fragments shorter than    3821 bp
  65% of sequence in fragments shorter than    4217 bp
  70% of sequence in fragments shorter than    4665 bp
  75% of sequence in fragments shorter than    5187 bp
  80% of sequence in fragments shorter than    5826 bp
  85% of sequence in fragments shorter than    6642 bp
  90% of sequence in fragments shorter than    7796 bp
  95% of sequence in fragments shorter than    9866 bp

# AccI.AvrII (GTMKAC+CCTAGG) double digest, 1695 thousand fragments (1101063+594956 sites), 1757 bp average fragment length
   5% of sequence in fragments shorter than     629 bp
  10% of sequence in fragments shorter than     938 bp
  15% of sequence in fragments shorter than    1195 bp
  20% of sequence in fragments shorter than    1447 bp
  25% of sequence in fragments shorter than    1699 bp
  30% of sequence in fragments shorter than    1946 bp
  35% of sequence in fragments shorter than    2203 bp
  40% of sequence in fragments shorter than    2465 bp
  45% of sequence in fragments shorter than    2736 bp
  50% of sequence in fragments shorter than    3021 bp
  55% of sequence in fragments shorter than    3330 bp
  60% of sequence in fragments shorter than    3663 bp
  65% of sequence in fragments shorter than    4025 bp
  70% of sequence in fragments shorter than    4422 bp
  75% of sequence in fragments shorter than    4892 bp
  80% of sequence in fragments shorter than    5467 bp
  85% of sequence in fragments shorter than    6210 bp
  90% of sequence in fragments shorter than    7267 bp
  95% of sequence in fragments shorter than    9158 bp

# AvaIII.NcoI (ATGCAT+CCATGG) double digest, 1692 thousand fragments (933357+759594 sites), 1760 bp average fragment length
   5% of sequence in fragments shorter than     620 bp
  10% of sequence in fragments shorter than     946 bp
  15% of sequence in fragments shorter than    1225 bp
  20% of sequence in fragments shorter than    1484 bp
  25% of sequence in fragments shorter than    1744 bp
  30% of sequence in fragments shorter than    2000 bp
  35% of sequence in fragments shorter than    2252 bp
  40% of sequence in fragments shorter than    2521 bp
  45% of sequence in fragments shorter than    2800 bp
  50% of sequence in fragments shorter than    3097 bp
  55% of sequence in fragments shorter than    3418 bp
  60% of sequence in fragments shorter than    3772 bp
  65% of sequence in fragments shorter than    4164 bp
  70% of sequence in fragments shorter than    4595 bp
  75% of sequence in fragments shorter than    5115 bp
  80% of sequence in fragments shorter than    5733 bp
  85% of sequence in fragments shorter than    6516 bp
  90% of sequence in fragments shorter than    7680 bp
  95% of sequence in fragments shorter than    9847 bp

# BsaAI.BspHI (YACGTR+TCATGA) double digest, 1691 thousand fragments (713801+978290 sites), 1761 bp average fragment length
   5% of sequence in fragments shorter than     633 bp
  10% of sequence in fragments shorter than     952 bp
  15% of sequence in fragments shorter than    1219 bp
  20% of sequence in fragments shorter than    1470 bp
  25% of sequence in fragments shorter than    1723 bp
  30% of sequence in fragments shorter than    1966 bp
  35% of sequence in fragments shorter than    2208 bp
  40% of sequence in fragments shorter than    2468 bp
  45% of sequence in fragments shorter than    2742 bp
  50% of sequence in fragments shorter than    3026 bp
  55% of sequence in fragments shorter than    3325 bp
  60% of sequence in fragments shorter than    3659 bp
  65% of sequence in fragments shorter than    4033 bp
  70% of sequence in fragments shorter than    4455 bp
  75% of sequence in fragments shorter than    4943 bp
  80% of sequence in fragments shorter than    5533 bp
  85% of sequence in fragments shorter than    6283 bp
  90% of sequence in fragments shorter than    7349 bp
  95% of sequence in fragments shorter than    9292 bp

# ApaI.BscGI (GGGCCC+CCCGT) double digest, 1690 thousand fragments (460339+1231070 sites), 1762 bp average fragment length
   5% of sequence in fragments shorter than     499 bp
  10% of sequence in fragments shorter than     867 bp
  15% of sequence in fragments shorter than    1238 bp
  20% of sequence in fragments shorter than    1609 bp
  25% of sequence in fragments shorter than    1991 bp
  30% of sequence in fragments shorter than    2390 bp
  35% of sequence in fragments shorter than    2806 bp
  40% of sequence in fragments shorter than    3251 bp
  45% of sequence in fragments shorter than    3728 bp
  50% of sequence in fragments shorter than    4246 bp
  55% of sequence in fragments shorter than    4810 bp
  60% of sequence in fragments shorter than    5444 bp
  65% of sequence in fragments shorter than    6150 bp
  70% of sequence in fragments shorter than    6952 bp
  75% of sequence in fragments shorter than    7906 bp
  80% of sequence in fragments shorter than    9092 bp
  85% of sequence in fragments shorter than   10582 bp
  90% of sequence in fragments shorter than   12727 bp
  95% of sequence in fragments shorter than   16393 bp

# PmeI.PsiI (GTTTAAAC+TTATAA) double digest, 1689 thousand fragments (42094+1647912 sites), 1763 bp average fragment length
   5% of sequence in fragments shorter than     586 bp
  10% of sequence in fragments shorter than     916 bp
  15% of sequence in fragments shorter than    1210 bp
  20% of sequence in fragments shorter than    1492 bp
  25% of sequence in fragments shorter than    1777 bp
  30% of sequence in fragments shorter than    2065 bp
  35% of sequence in fragments shorter than    2372 bp
  40% of sequence in fragments shorter than    2696 bp
  45% of sequence in fragments shorter than    3024 bp
  50% of sequence in fragments shorter than    3397 bp
  55% of sequence in fragments shorter than    3806 bp
  60% of sequence in fragments shorter than    4272 bp
  65% of sequence in fragments shorter than    4824 bp
  70% of sequence in fragments shorter than    5490 bp
  75% of sequence in fragments shorter than    6305 bp
  80% of sequence in fragments shorter than    7388 bp
  85% of sequence in fragments shorter than    8949 bp
  90% of sequence in fragments shorter than   11756 bp
  95% of sequence in fragments shorter than   19184 bp

# ClaI.EsaBC3I (ATCGAT+TCGA) double digest, 1689 thousand fragments (86477+1603346 sites), 1764 bp average fragment length
   5% of sequence in fragments shorter than     635 bp
  10% of sequence in fragments shorter than     972 bp
  15% of sequence in fragments shorter than    1272 bp
  20% of sequence in fragments shorter than    1551 bp
  25% of sequence in fragments shorter than    1834 bp
  30% of sequence in fragments shorter than    2123 bp
  35% of sequence in fragments shorter than    2421 bp
  40% of sequence in fragments shorter than    2730 bp
  45% of sequence in fragments shorter than    3051 bp
  50% of sequence in fragments shorter than    3392 bp
  55% of sequence in fragments shorter than    3765 bp
  60% of sequence in fragments shorter than    4172 bp
  65% of sequence in fragments shorter than    4630 bp
  70% of sequence in fragments shorter than    5147 bp
  75% of sequence in fragments shorter than    5757 bp
  80% of sequence in fragments shorter than    6494 bp
  85% of sequence in fragments shorter than    7438 bp
  90% of sequence in fragments shorter than    8738 bp
  95% of sequence in fragments shorter than   11016 bp

# PpuMI.SacI (RGGWCCY+GAGCTC) double digest, 1688 thousand fragments (1085138+603323 sites), 1765 bp average fragment length
   5% of sequence in fragments shorter than     574 bp
  10% of sequence in fragments shorter than     920 bp
  15% of sequence in fragments shorter than    1226 bp
  20% of sequence in fragments shorter than    1532 bp
  25% of sequence in fragments shorter than    1839 bp
  30% of sequence in fragments shorter than    2147 bp
  35% of sequence in fragments shorter than    2473 bp
  40% of sequence in fragments shorter than    2785 bp
  45% of sequence in fragments shorter than    3150 bp
  50% of sequence in fragments shorter than    3539 bp
  55% of sequence in fragments shorter than    3960 bp
  60% of sequence in fragments shorter than    4428 bp
  65% of sequence in fragments shorter than    4959 bp
  70% of sequence in fragments shorter than    5559 bp
  75% of sequence in fragments shorter than    6277 bp
  80% of sequence in fragments shorter than    7162 bp
  85% of sequence in fragments shorter than    8328 bp
  90% of sequence in fragments shorter than   10032 bp
  95% of sequence in fragments shorter than   13407 bp

# PvuII.SacI (CAGCTG+GAGCTC) double digest, 1687 thousand fragments (1084260+603323 sites), 1766 bp average fragment length
   5% of sequence in fragments shorter than     591 bp
  10% of sequence in fragments shorter than     925 bp
  15% of sequence in fragments shorter than    1216 bp
  20% of sequence in fragments shorter than    1500 bp
  25% of sequence in fragments shorter than    1784 bp
  30% of sequence in fragments shorter than    2079 bp
  35% of sequence in fragments shorter than    2380 bp
  40% of sequence in fragments shorter than    2702 bp
  45% of sequence in fragments shorter than    3038 bp
  50% of sequence in fragments shorter than    3406 bp
  55% of sequence in fragments shorter than    3809 bp
  60% of sequence in fragments shorter than    4254 bp
  65% of sequence in fragments shorter than    4739 bp
  70% of sequence in fragments shorter than    5298 bp
  75% of sequence in fragments shorter than    5952 bp
  80% of sequence in fragments shorter than    6754 bp
  85% of sequence in fragments shorter than    7802 bp
  90% of sequence in fragments shorter than    9325 bp
  95% of sequence in fragments shorter than   12263 bp

# BamHI.PstI (GGATCC+CTGCAG) double digest, 1687 thousand fragments (365999+1321469 sites), 1766 bp average fragment length
   5% of sequence in fragments shorter than     596 bp
  10% of sequence in fragments shorter than     920 bp
  15% of sequence in fragments shorter than    1218 bp
  20% of sequence in fragments shorter than    1498 bp
  25% of sequence in fragments shorter than    1786 bp
  30% of sequence in fragments shorter than    2075 bp
  35% of sequence in fragments shorter than    2375 bp
  40% of sequence in fragments shorter than    2693 bp
  45% of sequence in fragments shorter than    3022 bp
  50% of sequence in fragments shorter than    3379 bp
  55% of sequence in fragments shorter than    3737 bp
  60% of sequence in fragments shorter than    4148 bp
  65% of sequence in fragments shorter than    4629 bp
  70% of sequence in fragments shorter than    5177 bp
  75% of sequence in fragments shorter than    5822 bp
  80% of sequence in fragments shorter than    6603 bp
  85% of sequence in fragments shorter than    7629 bp
  90% of sequence in fragments shorter than    9100 bp
  95% of sequence in fragments shorter than   11818 bp

# NcoI.StuI (CCATGG+AGGCCT) double digest, 1684 thousand fragments (759594+925729 sites), 1768 bp average fragment length
   5% of sequence in fragments shorter than     568 bp
  10% of sequence in fragments shorter than     880 bp
  15% of sequence in fragments shorter than    1191 bp
  20% of sequence in fragments shorter than    1489 bp
  25% of sequence in fragments shorter than    1796 bp
  30% of sequence in fragments shorter than    2060 bp
  35% of sequence in fragments shorter than    2373 bp
  40% of sequence in fragments shorter than    2701 bp
  45% of sequence in fragments shorter than    3048 bp
  50% of sequence in fragments shorter than    3415 bp
  55% of sequence in fragments shorter than    3811 bp
  60% of sequence in fragments shorter than    4220 bp
  65% of sequence in fragments shorter than    4702 bp
  70% of sequence in fragments shorter than    5255 bp
  75% of sequence in fragments shorter than    5883 bp
  80% of sequence in fragments shorter than    6643 bp
  85% of sequence in fragments shorter than    7623 bp
  90% of sequence in fragments shorter than    8976 bp
  95% of sequence in fragments shorter than   11266 bp

# BceAI.BspHI (ACGGC+TCATGA) double digest, 1683 thousand fragments (705178+978290 sites), 1770 bp average fragment length
   5% of sequence in fragments shorter than     621 bp
  10% of sequence in fragments shorter than     948 bp
  15% of sequence in fragments shorter than    1235 bp
  20% of sequence in fragments shorter than    1506 bp
  25% of sequence in fragments shorter than    1771 bp
  30% of sequence in fragments shorter than    2029 bp
  35% of sequence in fragments shorter than    2288 bp
  40% of sequence in fragments shorter than    2563 bp
  45% of sequence in fragments shorter than    2851 bp
  50% of sequence in fragments shorter than    3147 bp
  55% of sequence in fragments shorter than    3473 bp
  60% of sequence in fragments shorter than    3815 bp
  65% of sequence in fragments shorter than    4192 bp
  70% of sequence in fragments shorter than    4633 bp
  75% of sequence in fragments shorter than    5152 bp
  80% of sequence in fragments shorter than    5775 bp
  85% of sequence in fragments shorter than    6573 bp
  90% of sequence in fragments shorter than    7715 bp
  95% of sequence in fragments shorter than    9780 bp

# HgiCI.SnaBI (GGYRCC+TACGTA) double digest, 1682 thousand fragments (1550877+132299 sites), 1770 bp average fragment length
   5% of sequence in fragments shorter than     590 bp
  10% of sequence in fragments shorter than     920 bp
  15% of sequence in fragments shorter than    1213 bp
  20% of sequence in fragments shorter than    1471 bp
  25% of sequence in fragments shorter than    1742 bp
  30% of sequence in fragments shorter than    2033 bp
  35% of sequence in fragments shorter than    2329 bp
  40% of sequence in fragments shorter than    2639 bp
  45% of sequence in fragments shorter than    2949 bp
  50% of sequence in fragments shorter than    3298 bp
  55% of sequence in fragments shorter than    3683 bp
  60% of sequence in fragments shorter than    4107 bp
  65% of sequence in fragments shorter than    4580 bp
  70% of sequence in fragments shorter than    5130 bp
  75% of sequence in fragments shorter than    5780 bp
  80% of sequence in fragments shorter than    6577 bp
  85% of sequence in fragments shorter than    7631 bp
  90% of sequence in fragments shorter than    9161 bp
  95% of sequence in fragments shorter than   12244 bp

# BsmI.SnaBI (GAATGC+TACGTA) double digest, 1681 thousand fragments (1549350+132299 sites), 1772 bp average fragment length
   5% of sequence in fragments shorter than     622 bp
  10% of sequence in fragments shorter than    1059 bp
  15% of sequence in fragments shorter than    1416 bp
  20% of sequence in fragments shorter than    1747 bp
  25% of sequence in fragments shorter than    2064 bp
  30% of sequence in fragments shorter than    2378 bp
  35% of sequence in fragments shorter than    2686 bp
  40% of sequence in fragments shorter than    3005 bp
  45% of sequence in fragments shorter than    3340 bp
  50% of sequence in fragments shorter than    3697 bp
  55% of sequence in fragments shorter than    4078 bp
  60% of sequence in fragments shorter than    4492 bp
  65% of sequence in fragments shorter than    4948 bp
  70% of sequence in fragments shorter than    5466 bp
  75% of sequence in fragments shorter than    6057 bp
  80% of sequence in fragments shorter than    6769 bp
  85% of sequence in fragments shorter than    7657 bp
  90% of sequence in fragments shorter than    8908 bp
  95% of sequence in fragments shorter than   10988 bp

# AvrII.PpuMI (CCTAGG+RGGWCCY) double digest, 1679 thousand fragments (594956+1085138 sites), 1774 bp average fragment length
   5% of sequence in fragments shorter than     602 bp
  10% of sequence in fragments shorter than     935 bp
  15% of sequence in fragments shorter than    1251 bp
  20% of sequence in fragments shorter than    1557 bp
  25% of sequence in fragments shorter than    1859 bp
  30% of sequence in fragments shorter than    2166 bp
  35% of sequence in fragments shorter than    2487 bp
  40% of sequence in fragments shorter than    2821 bp
  45% of sequence in fragments shorter than    3167 bp
  50% of sequence in fragments shorter than    3534 bp
  55% of sequence in fragments shorter than    3900 bp
  60% of sequence in fragments shorter than    4342 bp
  65% of sequence in fragments shorter than    4844 bp
  70% of sequence in fragments shorter than    5422 bp
  75% of sequence in fragments shorter than    6109 bp
  80% of sequence in fragments shorter than    6953 bp
  85% of sequence in fragments shorter than    8064 bp
  90% of sequence in fragments shorter than    9731 bp
  95% of sequence in fragments shorter than   13238 bp

# PsiI.SalI (TTATAA+GTCGAC) double digest, 1678 thousand fragments (1647912+31396 sites), 1774 bp average fragment length
   5% of sequence in fragments shorter than     592 bp
  10% of sequence in fragments shorter than     924 bp
  15% of sequence in fragments shorter than    1220 bp
  20% of sequence in fragments shorter than    1502 bp
  25% of sequence in fragments shorter than    1788 bp
  30% of sequence in fragments shorter than    2077 bp
  35% of sequence in fragments shorter than    2383 bp
  40% of sequence in fragments shorter than    2706 bp
  45% of sequence in fragments shorter than    3031 bp
  50% of sequence in fragments shorter than    3401 bp
  55% of sequence in fragments shorter than    3806 bp
  60% of sequence in fragments shorter than    4266 bp
  65% of sequence in fragments shorter than    4809 bp
  70% of sequence in fragments shorter than    5457 bp
  75% of sequence in fragments shorter than    6250 bp
  80% of sequence in fragments shorter than    7305 bp
  85% of sequence in fragments shorter than    8792 bp
  90% of sequence in fragments shorter than   11396 bp
  95% of sequence in fragments shorter than   18151 bp

# AvrII.PvuII (CCTAGG+CAGCTG) double digest, 1678 thousand fragments (594956+1084260 sites), 1775 bp average fragment length
   5% of sequence in fragments shorter than     610 bp
  10% of sequence in fragments shorter than     923 bp
  15% of sequence in fragments shorter than    1221 bp
  20% of sequence in fragments shorter than    1502 bp
  25% of sequence in fragments shorter than    1779 bp
  30% of sequence in fragments shorter than    2060 bp
  35% of sequence in fragments shorter than    2347 bp
  40% of sequence in fragments shorter than    2646 bp
  45% of sequence in fragments shorter than    2944 bp
  50% of sequence in fragments shorter than    3282 bp
  55% of sequence in fragments shorter than    3617 bp
  60% of sequence in fragments shorter than    4010 bp
  65% of sequence in fragments shorter than    4450 bp
  70% of sequence in fragments shorter than    4960 bp
  75% of sequence in fragments shorter than    5570 bp
  80% of sequence in fragments shorter than    6321 bp
  85% of sequence in fragments shorter than    7288 bp
  90% of sequence in fragments shorter than    8712 bp
  95% of sequence in fragments shorter than   11537 bp

# BglII.NdeI (AGATCT+CATATG) double digest, 1677 thousand fragments (774732+903360 sites), 1776 bp average fragment length
   5% of sequence in fragments shorter than     610 bp
  10% of sequence in fragments shorter than     920 bp
  15% of sequence in fragments shorter than    1184 bp
  20% of sequence in fragments shorter than    1452 bp
  25% of sequence in fragments shorter than    1694 bp
  30% of sequence in fragments shorter than    1954 bp
  35% of sequence in fragments shorter than    2223 bp
  40% of sequence in fragments shorter than    2489 bp
  45% of sequence in fragments shorter than    2783 bp
  50% of sequence in fragments shorter than    3098 bp
  55% of sequence in fragments shorter than    3437 bp
  60% of sequence in fragments shorter than    3814 bp
  65% of sequence in fragments shorter than    4232 bp
  70% of sequence in fragments shorter than    4710 bp
  75% of sequence in fragments shorter than    5279 bp
  80% of sequence in fragments shorter than    5992 bp
  85% of sequence in fragments shorter than    6944 bp
  90% of sequence in fragments shorter than    8390 bp
  95% of sequence in fragments shorter than   11453 bp

# BscGI.EcoRV (CCCGT+GATATC) double digest, 1677 thousand fragments (1231070+446473 sites), 1776 bp average fragment length
   5% of sequence in fragments shorter than     541 bp
  10% of sequence in fragments shorter than     900 bp
  15% of sequence in fragments shorter than    1231 bp
  20% of sequence in fragments shorter than    1547 bp
  25% of sequence in fragments shorter than    1854 bp
  30% of sequence in fragments shorter than    2159 bp
  35% of sequence in fragments shorter than    2473 bp
  40% of sequence in fragments shorter than    2801 bp
  45% of sequence in fragments shorter than    3148 bp
  50% of sequence in fragments shorter than    3516 bp
  55% of sequence in fragments shorter than    3910 bp
  60% of sequence in fragments shorter than    4339 bp
  65% of sequence in fragments shorter than    4815 bp
  70% of sequence in fragments shorter than    5358 bp
  75% of sequence in fragments shorter than    5985 bp
  80% of sequence in fragments shorter than    6737 bp
  85% of sequence in fragments shorter than    7699 bp
  90% of sequence in fragments shorter than    9035 bp
  95% of sequence in fragments shorter than   11281 bp

# HgiCI.NaeI (GGYRCC+GCCGGC) double digest, 1676 thousand fragments (1550877+125692 sites), 1777 bp average fragment length
   5% of sequence in fragments shorter than     584 bp
  10% of sequence in fragments shorter than     932 bp
  15% of sequence in fragments shorter than    1226 bp
  20% of sequence in fragments shorter than    1529 bp
  25% of sequence in fragments shorter than    1824 bp
  30% of sequence in fragments shorter than    2142 bp
  35% of sequence in fragments shorter than    2472 bp
  40% of sequence in fragments shorter than    2810 bp
  45% of sequence in fragments shorter than    3161 bp
  50% of sequence in fragments shorter than    3558 bp
  55% of sequence in fragments shorter than    3993 bp
  60% of sequence in fragments shorter than    4469 bp
  65% of sequence in fragments shorter than    5017 bp
  70% of sequence in fragments shorter than    5648 bp
  75% of sequence in fragments shorter than    6400 bp
  80% of sequence in fragments shorter than    7346 bp
  85% of sequence in fragments shorter than    8579 bp
  90% of sequence in fragments shorter than   10426 bp
  95% of sequence in fragments shorter than   14298 bp

# BsrBI.FauI (CCGCTC+CCCGC) double digest, 1674 thousand fragments (235523+1439772 sites), 1779 bp average fragment length
   5% of sequence in fragments shorter than     645 bp
  10% of sequence in fragments shorter than    1148 bp
  15% of sequence in fragments shorter than    1621 bp
  20% of sequence in fragments shorter than    2089 bp
  25% of sequence in fragments shorter than    2589 bp
  30% of sequence in fragments shorter than    3121 bp
  35% of sequence in fragments shorter than    3693 bp
  40% of sequence in fragments shorter than    4303 bp
  45% of sequence in fragments shorter than    4975 bp
  50% of sequence in fragments shorter than    5715 bp
  55% of sequence in fragments shorter than    6528 bp
  60% of sequence in fragments shorter than    7446 bp
  65% of sequence in fragments shorter than    8479 bp
  70% of sequence in fragments shorter than    9692 bp
  75% of sequence in fragments shorter than   11126 bp
  80% of sequence in fragments shorter than   12886 bp
  85% of sequence in fragments shorter than   15198 bp
  90% of sequence in fragments shorter than   18605 bp
  95% of sequence in fragments shorter than   24705 bp

# BsmI.NaeI (GAATGC+GCCGGC) double digest, 1674 thousand fragments (1549350+125692 sites), 1779 bp average fragment length
   5% of sequence in fragments shorter than     617 bp
  10% of sequence in fragments shorter than    1062 bp
  15% of sequence in fragments shorter than    1427 bp
  20% of sequence in fragments shorter than    1765 bp
  25% of sequence in fragments shorter than    2089 bp
  30% of sequence in fragments shorter than    2410 bp
  35% of sequence in fragments shorter than    2727 bp
  40% of sequence in fragments shorter than    3047 bp
  45% of sequence in fragments shorter than    3391 bp
  50% of sequence in fragments shorter than    3753 bp
  55% of sequence in fragments shorter than    4139 bp
  60% of sequence in fragments shorter than    4560 bp
  65% of sequence in fragments shorter than    5023 bp
  70% of sequence in fragments shorter than    5549 bp
  75% of sequence in fragments shorter than    6147 bp
  80% of sequence in fragments shorter than    6862 bp
  85% of sequence in fragments shorter than    7753 bp
  90% of sequence in fragments shorter than    8996 bp
  95% of sequence in fragments shorter than   11062 bp

# BciVI.BspHI (GTATCC+TCATGA) double digest, 1673 thousand fragments (696093+978290 sites), 1780 bp average fragment length
   5% of sequence in fragments shorter than     632 bp
  10% of sequence in fragments shorter than     951 bp
  15% of sequence in fragments shorter than    1227 bp
  20% of sequence in fragments shorter than    1492 bp
  25% of sequence in fragments shorter than    1745 bp
  30% of sequence in fragments shorter than    1992 bp
  35% of sequence in fragments shorter than    2247 bp
  40% of sequence in fragments shorter than    2515 bp
  45% of sequence in fragments shorter than    2794 bp
  50% of sequence in fragments shorter than    3093 bp
  55% of sequence in fragments shorter than    3408 bp
  60% of sequence in fragments shorter than    3758 bp
  65% of sequence in fragments shorter than    4147 bp
  70% of sequence in fragments shorter than    4588 bp
  75% of sequence in fragments shorter than    5104 bp
  80% of sequence in fragments shorter than    5728 bp
  85% of sequence in fragments shorter than    6545 bp
  90% of sequence in fragments shorter than    7738 bp
  95% of sequence in fragments shorter than   10041 bp

# AatII.EsaBC3I (GACGTC+TCGA) double digest, 1673 thousand fragments (71089+1603346 sites), 1780 bp average fragment length
   5% of sequence in fragments shorter than     607 bp
  10% of sequence in fragments shorter than     930 bp
  15% of sequence in fragments shorter than    1218 bp
  20% of sequence in fragments shorter than    1485 bp
  25% of sequence in fragments shorter than    1760 bp
  30% of sequence in fragments shorter than    2038 bp
  35% of sequence in fragments shorter than    2324 bp
  40% of sequence in fragments shorter than    2620 bp
  45% of sequence in fragments shorter than    2932 bp
  50% of sequence in fragments shorter than    3258 bp
  55% of sequence in fragments shorter than    3616 bp
  60% of sequence in fragments shorter than    4009 bp
  65% of sequence in fragments shorter than    4451 bp
  70% of sequence in fragments shorter than    4947 bp
  75% of sequence in fragments shorter than    5537 bp
  80% of sequence in fragments shorter than    6246 bp
  85% of sequence in fragments shorter than    7160 bp
  90% of sequence in fragments shorter than    8418 bp
  95% of sequence in fragments shorter than   10594 bp

# BspMI.BsrBI (ACCTGC+CCGCTC) double digest, 1673 thousand fragments (1438129+235523 sites), 1781 bp average fragment length
   5% of sequence in fragments shorter than     591 bp
  10% of sequence in fragments shorter than     925 bp
  15% of sequence in fragments shorter than    1239 bp
  20% of sequence in fragments shorter than    1531 bp
  25% of sequence in fragments shorter than    1836 bp
  30% of sequence in fragments shorter than    2112 bp
  35% of sequence in fragments shorter than    2424 bp
  40% of sequence in fragments shorter than    2753 bp
  45% of sequence in fragments shorter than    3095 bp
  50% of sequence in fragments shorter than    3414 bp
  55% of sequence in fragments shorter than    3809 bp
  60% of sequence in fragments shorter than    4245 bp
  65% of sequence in fragments shorter than    4731 bp
  70% of sequence in fragments shorter than    5281 bp
  75% of sequence in fragments shorter than    5928 bp
  80% of sequence in fragments shorter than    6706 bp
  85% of sequence in fragments shorter than    7699 bp
  90% of sequence in fragments shorter than    9099 bp
  95% of sequence in fragments shorter than   11483 bp

# AccI.HgaI (GTMKAC+GACGC) double digest, 1672 thousand fragments (1101063+571889 sites), 1781 bp average fragment length
   5% of sequence in fragments shorter than     624 bp
  10% of sequence in fragments shorter than     966 bp
  15% of sequence in fragments shorter than    1259 bp
  20% of sequence in fragments shorter than    1534 bp
  25% of sequence in fragments shorter than    1805 bp
  30% of sequence in fragments shorter than    2071 bp
  35% of sequence in fragments shorter than    2344 bp
  40% of sequence in fragments shorter than    2621 bp
  45% of sequence in fragments shorter than    2897 bp
  50% of sequence in fragments shorter than    3198 bp
  55% of sequence in fragments shorter than    3529 bp
  60% of sequence in fragments shorter than    3887 bp
  65% of sequence in fragments shorter than    4292 bp
  70% of sequence in fragments shorter than    4737 bp
  75% of sequence in fragments shorter than    5255 bp
  80% of sequence in fragments shorter than    5861 bp
  85% of sequence in fragments shorter than    6644 bp
  90% of sequence in fragments shorter than    7758 bp
  95% of sequence in fragments shorter than    9762 bp

# AccI.EciI (GTMKAC+GGCGGA) double digest, 1671 thousand fragments (1101063+571273 sites), 1782 bp average fragment length
   5% of sequence in fragments shorter than     614 bp
  10% of sequence in fragments shorter than     945 bp
  15% of sequence in fragments shorter than    1229 bp
  20% of sequence in fragments shorter than    1493 bp
  25% of sequence in fragments shorter than    1757 bp
  30% of sequence in fragments shorter than    2017 bp
  35% of sequence in fragments shorter than    2283 bp
  40% of sequence in fragments shorter than    2560 bp
  45% of sequence in fragments shorter than    2844 bp
  50% of sequence in fragments shorter than    3152 bp
  55% of sequence in fragments shorter than    3481 bp
  60% of sequence in fragments shorter than    3842 bp
  65% of sequence in fragments shorter than    4249 bp
  70% of sequence in fragments shorter than    4694 bp
  75% of sequence in fragments shorter than    5223 bp
  80% of sequence in fragments shorter than    5827 bp
  85% of sequence in fragments shorter than    6581 bp
  90% of sequence in fragments shorter than    7712 bp
  95% of sequence in fragments shorter than    9772 bp

# HgiCI.XhoI (GGYRCC+CTCGAG) double digest, 1671 thousand fragments (1550877+121308 sites), 1782 bp average fragment length
   5% of sequence in fragments shorter than     580 bp
  10% of sequence in fragments shorter than     913 bp
  15% of sequence in fragments shorter than    1216 bp
  20% of sequence in fragments shorter than    1496 bp
  25% of sequence in fragments shorter than    1780 bp
  30% of sequence in fragments shorter than    2081 bp
  35% of sequence in fragments shorter than    2398 bp
  40% of sequence in fragments shorter than    2728 bp
  45% of sequence in fragments shorter than    3071 bp
  50% of sequence in fragments shorter than    3451 bp
  55% of sequence in fragments shorter than    3867 bp
  60% of sequence in fragments shorter than    4330 bp
  65% of sequence in fragments shorter than    4851 bp
  70% of sequence in fragments shorter than    5462 bp
  75% of sequence in fragments shorter than    6182 bp
  80% of sequence in fragments shorter than    7082 bp
  85% of sequence in fragments shorter than    8267 bp
  90% of sequence in fragments shorter than   10034 bp
  95% of sequence in fragments shorter than   13706 bp

# AvaIII.BclI (ATGCAT+TGATCA) double digest, 1671 thousand fragments (933357+738785 sites), 1782 bp average fragment length
   5% of sequence in fragments shorter than     631 bp
  10% of sequence in fragments shorter than     949 bp
  15% of sequence in fragments shorter than    1233 bp
  20% of sequence in fragments shorter than    1499 bp
  25% of sequence in fragments shorter than    1756 bp
  30% of sequence in fragments shorter than    2017 bp
  35% of sequence in fragments shorter than    2276 bp
  40% of sequence in fragments shorter than    2547 bp
  45% of sequence in fragments shorter than    2822 bp
  50% of sequence in fragments shorter than    3128 bp
  55% of sequence in fragments shorter than    3456 bp
  60% of sequence in fragments shorter than    3817 bp
  65% of sequence in fragments shorter than    4208 bp
  70% of sequence in fragments shorter than    4661 bp
  75% of sequence in fragments shorter than    5192 bp
  80% of sequence in fragments shorter than    5857 bp
  85% of sequence in fragments shorter than    6727 bp
  90% of sequence in fragments shorter than    8016 bp
  95% of sequence in fragments shorter than   10614 bp

# BsmI.XhoI (GAATGC+CTCGAG) double digest, 1670 thousand fragments (1549350+121308 sites), 1784 bp average fragment length
   5% of sequence in fragments shorter than     623 bp
  10% of sequence in fragments shorter than    1066 bp
  15% of sequence in fragments shorter than    1423 bp
  20% of sequence in fragments shorter than    1762 bp
  25% of sequence in fragments shorter than    2081 bp
  30% of sequence in fragments shorter than    2400 bp
  35% of sequence in fragments shorter than    2702 bp
  40% of sequence in fragments shorter than    3024 bp
  45% of sequence in fragments shorter than    3365 bp
  50% of sequence in fragments shorter than    3723 bp
  55% of sequence in fragments shorter than    4106 bp
  60% of sequence in fragments shorter than    4521 bp
  65% of sequence in fragments shorter than    4976 bp
  70% of sequence in fragments shorter than    5498 bp
  75% of sequence in fragments shorter than    6088 bp
  80% of sequence in fragments shorter than    6796 bp
  85% of sequence in fragments shorter than    7678 bp
  90% of sequence in fragments shorter than    8914 bp
  95% of sequence in fragments shorter than   10943 bp

# FauI.KasI (CCCGC+GGCGCC) double digest, 1669 thousand fragments (1439772+230381 sites), 1784 bp average fragment length
   5% of sequence in fragments shorter than     636 bp
  10% of sequence in fragments shorter than    1140 bp
  15% of sequence in fragments shorter than    1617 bp
  20% of sequence in fragments shorter than    2101 bp
  25% of sequence in fragments shorter than    2622 bp
  30% of sequence in fragments shorter than    3175 bp
  35% of sequence in fragments shorter than    3762 bp
  40% of sequence in fragments shorter than    4363 bp
  45% of sequence in fragments shorter than    5059 bp
  50% of sequence in fragments shorter than    5823 bp
  55% of sequence in fragments shorter than    6680 bp
  60% of sequence in fragments shorter than    7629 bp
  65% of sequence in fragments shorter than    8703 bp
  70% of sequence in fragments shorter than    9970 bp
  75% of sequence in fragments shorter than   11463 bp
  80% of sequence in fragments shorter than   13310 bp
  85% of sequence in fragments shorter than   15803 bp
  90% of sequence in fragments shorter than   19460 bp
  95% of sequence in fragments shorter than   26393 bp

# EsaBC3I.SacII (TCGA+CCGCGG) double digest, 1669 thousand fragments (1603346+66901 sites), 1784 bp average fragment length
   5% of sequence in fragments shorter than     604 bp
  10% of sequence in fragments shorter than     936 bp
  15% of sequence in fragments shorter than    1234 bp
  20% of sequence in fragments shorter than    1508 bp
  25% of sequence in fragments shorter than    1790 bp
  30% of sequence in fragments shorter than    2077 bp
  35% of sequence in fragments shorter than    2371 bp
  40% of sequence in fragments shorter than    2677 bp
  45% of sequence in fragments shorter than    2993 bp
  50% of sequence in fragments shorter than    3333 bp
  55% of sequence in fragments shorter than    3702 bp
  60% of sequence in fragments shorter than    4107 bp
  65% of sequence in fragments shorter than    4563 bp
  70% of sequence in fragments shorter than    5074 bp
  75% of sequence in fragments shorter than    5682 bp
  80% of sequence in fragments shorter than    6418 bp
  85% of sequence in fragments shorter than    7358 bp
  90% of sequence in fragments shorter than    8649 bp
  95% of sequence in fragments shorter than   10929 bp

# MluI.PsiI (ACGCGT+TTATAA) double digest, 1668 thousand fragments (21415+1647912 sites), 1785 bp average fragment length
   5% of sequence in fragments shorter than     596 bp
  10% of sequence in fragments shorter than     930 bp
  15% of sequence in fragments shorter than    1229 bp
  20% of sequence in fragments shorter than    1513 bp
  25% of sequence in fragments shorter than    1801 bp
  30% of sequence in fragments shorter than    2093 bp
  35% of sequence in fragments shorter than    2401 bp
  40% of sequence in fragments shorter than    2727 bp
  45% of sequence in fragments shorter than    3054 bp
  50% of sequence in fragments shorter than    3427 bp
  55% of sequence in fragments shorter than    3837 bp
  60% of sequence in fragments shorter than    4302 bp
  65% of sequence in fragments shorter than    4846 bp
  70% of sequence in fragments shorter than    5498 bp
  75% of sequence in fragments shorter than    6297 bp
  80% of sequence in fragments shorter than    7342 bp
  85% of sequence in fragments shorter than    8820 bp
  90% of sequence in fragments shorter than   11381 bp
  95% of sequence in fragments shorter than   17796 bp

# BspMI.KasI (ACCTGC+GGCGCC) double digest, 1668 thousand fragments (1438129+230381 sites), 1786 bp average fragment length
   5% of sequence in fragments shorter than     592 bp
  10% of sequence in fragments shorter than     923 bp
  15% of sequence in fragments shorter than    1231 bp
  20% of sequence in fragments shorter than    1518 bp
  25% of sequence in fragments shorter than    1814 bp
  30% of sequence in fragments shorter than    2086 bp
  35% of sequence in fragments shorter than    2389 bp
  40% of sequence in fragments shorter than    2706 bp
  45% of sequence in fragments shorter than    3038 bp
  50% of sequence in fragments shorter than    3349 bp
  55% of sequence in fragments shorter than    3732 bp
  60% of sequence in fragments shorter than    4159 bp
  65% of sequence in fragments shorter than    4634 bp
  70% of sequence in fragments shorter than    5172 bp
  75% of sequence in fragments shorter than    5793 bp
  80% of sequence in fragments shorter than    6554 bp
  85% of sequence in fragments shorter than    7536 bp
  90% of sequence in fragments shorter than    8946 bp
  95% of sequence in fragments shorter than   11385 bp

# AvaIII.FnuDII (ATGCAT+CGCG) double digest, 1666 thousand fragments (933357+733940 sites), 1787 bp average fragment length
   5% of sequence in fragments shorter than     682 bp
  10% of sequence in fragments shorter than    1094 bp
  15% of sequence in fragments shorter than    1448 bp
  20% of sequence in fragments shorter than    1780 bp
  25% of sequence in fragments shorter than    2093 bp
  30% of sequence in fragments shorter than    2414 bp
  35% of sequence in fragments shorter than    2740 bp
  40% of sequence in fragments shorter than    3078 bp
  45% of sequence in fragments shorter than    3432 bp
  50% of sequence in fragments shorter than    3811 bp
  55% of sequence in fragments shorter than    4221 bp
  60% of sequence in fragments shorter than    4664 bp
  65% of sequence in fragments shorter than    5161 bp
  70% of sequence in fragments shorter than    5723 bp
  75% of sequence in fragments shorter than    6374 bp
  80% of sequence in fragments shorter than    7169 bp
  85% of sequence in fragments shorter than    8196 bp
  90% of sequence in fragments shorter than    9671 bp
  95% of sequence in fragments shorter than   12516 bp

# FauI.SwaI (CCCGC+ATTTAAAT) double digest, 1665 thousand fragments (1439772+226602 sites), 1788 bp average fragment length
   5% of sequence in fragments shorter than     633 bp
  10% of sequence in fragments shorter than    1079 bp
  15% of sequence in fragments shorter than    1481 bp
  20% of sequence in fragments shorter than    1887 bp
  25% of sequence in fragments shorter than    2285 bp
  30% of sequence in fragments shorter than    2700 bp
  35% of sequence in fragments shorter than    3136 bp
  40% of sequence in fragments shorter than    3593 bp
  45% of sequence in fragments shorter than    4079 bp
  50% of sequence in fragments shorter than    4579 bp
  55% of sequence in fragments shorter than    5157 bp
  60% of sequence in fragments shorter than    5795 bp
  65% of sequence in fragments shorter than    6510 bp
  70% of sequence in fragments shorter than    7318 bp
  75% of sequence in fragments shorter than    8280 bp
  80% of sequence in fragments shorter than    9473 bp
  85% of sequence in fragments shorter than   11023 bp
  90% of sequence in fragments shorter than   13237 bp
  95% of sequence in fragments shorter than   17473 bp

# AccI.MfeI (GTMKAC+CAATTG) double digest, 1664 thousand fragments (1101063+564303 sites), 1789 bp average fragment length
   5% of sequence in fragments shorter than     630 bp
  10% of sequence in fragments shorter than     949 bp
  15% of sequence in fragments shorter than    1225 bp
  20% of sequence in fragments shorter than    1487 bp
  25% of sequence in fragments shorter than    1724 bp
  30% of sequence in fragments shorter than    1977 bp
  35% of sequence in fragments shorter than    2237 bp
  40% of sequence in fragments shorter than    2512 bp
  45% of sequence in fragments shorter than    2797 bp
  50% of sequence in fragments shorter than    3105 bp
  55% of sequence in fragments shorter than    3437 bp
  60% of sequence in fragments shorter than    3796 bp
  65% of sequence in fragments shorter than    4191 bp
  70% of sequence in fragments shorter than    4620 bp
  75% of sequence in fragments shorter than    5158 bp
  80% of sequence in fragments shorter than    5809 bp
  85% of sequence in fragments shorter than    6663 bp
  90% of sequence in fragments shorter than    7910 bp
  95% of sequence in fragments shorter than   10252 bp

# BspMI.SwaI (ACCTGC+ATTTAAAT) double digest, 1664 thousand fragments (1438129+226602 sites), 1790 bp average fragment length
   5% of sequence in fragments shorter than     617 bp
  10% of sequence in fragments shorter than     938 bp
  15% of sequence in fragments shorter than    1232 bp
  20% of sequence in fragments shorter than    1504 bp
  25% of sequence in fragments shorter than    1779 bp
  30% of sequence in fragments shorter than    2032 bp
  35% of sequence in fragments shorter than    2307 bp
  40% of sequence in fragments shorter than    2595 bp
  45% of sequence in fragments shorter than    2892 bp
  50% of sequence in fragments shorter than    3212 bp
  55% of sequence in fragments shorter than    3511 bp
  60% of sequence in fragments shorter than    3881 bp
  65% of sequence in fragments shorter than    4290 bp
  70% of sequence in fragments shorter than    4758 bp
  75% of sequence in fragments shorter than    5290 bp
  80% of sequence in fragments shorter than    5935 bp
  85% of sequence in fragments shorter than    6765 bp
  90% of sequence in fragments shorter than    7945 bp
  95% of sequence in fragments shorter than    9938 bp

# BclI.StuI (TGATCA+AGGCCT) double digest, 1664 thousand fragments (738785+925729 sites), 1790 bp average fragment length
   5% of sequence in fragments shorter than     605 bp
  10% of sequence in fragments shorter than     914 bp
  15% of sequence in fragments shorter than    1220 bp
  20% of sequence in fragments shorter than    1495 bp
  25% of sequence in fragments shorter than    1774 bp
  30% of sequence in fragments shorter than    2031 bp
  35% of sequence in fragments shorter than    2318 bp
  40% of sequence in fragments shorter than    2606 bp
  45% of sequence in fragments shorter than    2905 bp
  50% of sequence in fragments shorter than    3224 bp
  55% of sequence in fragments shorter than    3563 bp
  60% of sequence in fragments shorter than    3930 bp
  65% of sequence in fragments shorter than    4334 bp
  70% of sequence in fragments shorter than    4792 bp
  75% of sequence in fragments shorter than    5323 bp
  80% of sequence in fragments shorter than    5961 bp
  85% of sequence in fragments shorter than    6772 bp
  90% of sequence in fragments shorter than    7867 bp
  95% of sequence in fragments shorter than    9759 bp

# PsiI.SgrAI (TTATAA+CRCCGGYG) double digest, 1662 thousand fragments (1647912+15270 sites), 1792 bp average fragment length
   5% of sequence in fragments shorter than     598 bp
  10% of sequence in fragments shorter than     934 bp
  15% of sequence in fragments shorter than    1233 bp
  20% of sequence in fragments shorter than    1518 bp
  25% of sequence in fragments shorter than    1807 bp
  30% of sequence in fragments shorter than    2100 bp
  35% of sequence in fragments shorter than    2409 bp
  40% of sequence in fragments shorter than    2736 bp
  45% of sequence in fragments shorter than    3064 bp
  50% of sequence in fragments shorter than    3438 bp
  55% of sequence in fragments shorter than    3850 bp
  60% of sequence in fragments shorter than    4319 bp
  65% of sequence in fragments shorter than    4868 bp
  70% of sequence in fragments shorter than    5526 bp
  75% of sequence in fragments shorter than    6330 bp
  80% of sequence in fragments shorter than    7389 bp
  85% of sequence in fragments shorter than    8880 bp
  90% of sequence in fragments shorter than   11490 bp
  95% of sequence in fragments shorter than   17972 bp

# NcoI.NdeI (CCATGG+CATATG) double digest, 1662 thousand fragments (759594+903360 sites), 1792 bp average fragment length
   5% of sequence in fragments shorter than     617 bp
  10% of sequence in fragments shorter than     930 bp
  15% of sequence in fragments shorter than    1218 bp
  20% of sequence in fragments shorter than    1493 bp
  25% of sequence in fragments shorter than    1765 bp
  30% of sequence in fragments shorter than    2034 bp
  35% of sequence in fragments shorter than    2306 bp
  40% of sequence in fragments shorter than    2586 bp
  45% of sequence in fragments shorter than    2878 bp
  50% of sequence in fragments shorter than    3192 bp
  55% of sequence in fragments shorter than    3525 bp
  60% of sequence in fragments shorter than    3889 bp
  65% of sequence in fragments shorter than    4292 bp
  70% of sequence in fragments shorter than    4748 bp
  75% of sequence in fragments shorter than    5275 bp
  80% of sequence in fragments shorter than    5927 bp
  85% of sequence in fragments shorter than    6774 bp
  90% of sequence in fragments shorter than    8019 bp
  95% of sequence in fragments shorter than   10432 bp

# NruI.PsiI (TCGCGA+TTATAA) double digest, 1662 thousand fragments (14872+1647912 sites), 1792 bp average fragment length
   5% of sequence in fragments shorter than     597 bp
  10% of sequence in fragments shorter than     933 bp
  15% of sequence in fragments shorter than    1232 bp
  20% of sequence in fragments shorter than    1517 bp
  25% of sequence in fragments shorter than    1806 bp
  30% of sequence in fragments shorter than    2098 bp
  35% of sequence in fragments shorter than    2409 bp
  40% of sequence in fragments shorter than    2736 bp
  45% of sequence in fragments shorter than    3065 bp
  50% of sequence in fragments shorter than    3440 bp
  55% of sequence in fragments shorter than    3854 bp
  60% of sequence in fragments shorter than    4325 bp
  65% of sequence in fragments shorter than    4878 bp
  70% of sequence in fragments shorter than    5539 bp
  75% of sequence in fragments shorter than    6360 bp
  80% of sequence in fragments shorter than    7437 bp
  85% of sequence in fragments shorter than    8969 bp
  90% of sequence in fragments shorter than   11694 bp
  95% of sequence in fragments shorter than   18686 bp

# EsaBC3I.PspXI (TCGA+VCTCGAGB) double digest, 1662 thousand fragments (1603346+59193 sites), 1792 bp average fragment length
   5% of sequence in fragments shorter than     635 bp
  10% of sequence in fragments shorter than     972 bp
  15% of sequence in fragments shorter than    1272 bp
  20% of sequence in fragments shorter than    1551 bp
  25% of sequence in fragments shorter than    1834 bp
  30% of sequence in fragments shorter than    2123 bp
  35% of sequence in fragments shorter than    2421 bp
  40% of sequence in fragments shorter than    2730 bp
  45% of sequence in fragments shorter than    3051 bp
  50% of sequence in fragments shorter than    3392 bp
  55% of sequence in fragments shorter than    3765 bp
  60% of sequence in fragments shorter than    4172 bp
  65% of sequence in fragments shorter than    4630 bp
  70% of sequence in fragments shorter than    5147 bp
  75% of sequence in fragments shorter than    5757 bp
  80% of sequence in fragments shorter than    6494 bp
  85% of sequence in fragments shorter than    7439 bp
  90% of sequence in fragments shorter than    8737 bp
  95% of sequence in fragments shorter than   11017 bp

# PsiI.PvuI (TTATAA+CGATCG) double digest, 1661 thousand fragments (1647912+14186 sites), 1793 bp average fragment length
   5% of sequence in fragments shorter than     598 bp
  10% of sequence in fragments shorter than     934 bp
  15% of sequence in fragments shorter than    1234 bp
  20% of sequence in fragments shorter than    1519 bp
  25% of sequence in fragments shorter than    1808 bp
  30% of sequence in fragments shorter than    2098 bp
  35% of sequence in fragments shorter than    2409 bp
  40% of sequence in fragments shorter than    2737 bp
  45% of sequence in fragments shorter than    3066 bp
  50% of sequence in fragments shorter than    3441 bp
  55% of sequence in fragments shorter than    3854 bp
  60% of sequence in fragments shorter than    4324 bp
  65% of sequence in fragments shorter than    4875 bp
  70% of sequence in fragments shorter than    5536 bp
  75% of sequence in fragments shorter than    6352 bp
  80% of sequence in fragments shorter than    7428 bp
  85% of sequence in fragments shorter than    8953 bp
  90% of sequence in fragments shorter than   11666 bp
  95% of sequence in fragments shorter than   18590 bp

# FseI.PsiI (GGCCGGCC+TTATAA) double digest, 1661 thousand fragments (13662+1647912 sites), 1793 bp average fragment length
   5% of sequence in fragments shorter than     598 bp
  10% of sequence in fragments shorter than     935 bp
  15% of sequence in fragments shorter than    1235 bp
  20% of sequence in fragments shorter than    1520 bp
  25% of sequence in fragments shorter than    1810 bp
  30% of sequence in fragments shorter than    2103 bp
  35% of sequence in fragments shorter than    2413 bp
  40% of sequence in fragments shorter than    2741 bp
  45% of sequence in fragments shorter than    3070 bp
  50% of sequence in fragments shorter than    3444 bp
  55% of sequence in fragments shorter than    3856 bp
  60% of sequence in fragments shorter than    4324 bp
  65% of sequence in fragments shorter than    4871 bp
  70% of sequence in fragments shorter than    5528 bp
  75% of sequence in fragments shorter than    6332 bp
  80% of sequence in fragments shorter than    7388 bp
  85% of sequence in fragments shorter than    8865 bp
  90% of sequence in fragments shorter than   11420 bp
  95% of sequence in fragments shorter than   17665 bp

# FnuDII.StuI (CGCG+AGGCCT) double digest, 1659 thousand fragments (733940+925729 sites), 1796 bp average fragment length
   5% of sequence in fragments shorter than     605 bp
  10% of sequence in fragments shorter than    1017 bp
  15% of sequence in fragments shorter than    1417 bp
  20% of sequence in fragments shorter than    1825 bp
  25% of sequence in fragments shorter than    2209 bp
  30% of sequence in fragments shorter than    2629 bp
  35% of sequence in fragments shorter than    3064 bp
  40% of sequence in fragments shorter than    3510 bp
  45% of sequence in fragments shorter than    3987 bp
  50% of sequence in fragments shorter than    4503 bp
  55% of sequence in fragments shorter than    5062 bp
  60% of sequence in fragments shorter than    5678 bp
  65% of sequence in fragments shorter than    6366 bp
  70% of sequence in fragments shorter than    7159 bp
  75% of sequence in fragments shorter than    8072 bp
  80% of sequence in fragments shorter than    9158 bp
  85% of sequence in fragments shorter than   10569 bp
  90% of sequence in fragments shorter than   12525 bp
  95% of sequence in fragments shorter than   15978 bp

# AclI.AflIII (AACGTT+ACRYGT) double digest, 1657 thousand fragments (172706+1485394 sites), 1797 bp average fragment length
   5% of sequence in fragments shorter than     648 bp
  10% of sequence in fragments shorter than    1002 bp
  15% of sequence in fragments shorter than    1301 bp
  20% of sequence in fragments shorter than    1592 bp
  25% of sequence in fragments shorter than    1873 bp
  30% of sequence in fragments shorter than    2139 bp
  35% of sequence in fragments shorter than    2421 bp
  40% of sequence in fragments shorter than    2713 bp
  45% of sequence in fragments shorter than    3011 bp
  50% of sequence in fragments shorter than    3330 bp
  55% of sequence in fragments shorter than    3669 bp
  60% of sequence in fragments shorter than    4046 bp
  65% of sequence in fragments shorter than    4460 bp
  70% of sequence in fragments shorter than    4932 bp
  75% of sequence in fragments shorter than    5484 bp
  80% of sequence in fragments shorter than    6140 bp
  85% of sequence in fragments shorter than    6985 bp
  90% of sequence in fragments shorter than    8189 bp
  95% of sequence in fragments shorter than   10363 bp

# NotI.PsiI (GCGGCCGC+TTATAA) double digest, 1657 thousand fragments (9795+1647912 sites), 1798 bp average fragment length
   5% of sequence in fragments shorter than     599 bp
  10% of sequence in fragments shorter than     936 bp
  15% of sequence in fragments shorter than    1237 bp
  20% of sequence in fragments shorter than    1523 bp
  25% of sequence in fragments shorter than    1814 bp
  30% of sequence in fragments shorter than    2108 bp
  35% of sequence in fragments shorter than    2419 bp
  40% of sequence in fragments shorter than    2749 bp
  45% of sequence in fragments shorter than    3079 bp
  50% of sequence in fragments shorter than    3455 bp
  55% of sequence in fragments shorter than    3870 bp
  60% of sequence in fragments shorter than    4343 bp
  65% of sequence in fragments shorter than    4897 bp
  70% of sequence in fragments shorter than    5561 bp
  75% of sequence in fragments shorter than    6381 bp
  80% of sequence in fragments shorter than    7461 bp
  85% of sequence in fragments shorter than    8982 bp
  90% of sequence in fragments shorter than   11683 bp
  95% of sequence in fragments shorter than   18551 bp

# PsiI.RsrII (TTATAA+CGGWCCG) double digest, 1657 thousand fragments (1647912+9764 sites), 1798 bp average fragment length
   5% of sequence in fragments shorter than     599 bp
  10% of sequence in fragments shorter than     936 bp
  15% of sequence in fragments shorter than    1237 bp
  20% of sequence in fragments shorter than    1523 bp
  25% of sequence in fragments shorter than    1813 bp
  30% of sequence in fragments shorter than    2107 bp
  35% of sequence in fragments shorter than    2418 bp
  40% of sequence in fragments shorter than    2748 bp
  45% of sequence in fragments shorter than    3078 bp
  50% of sequence in fragments shorter than    3454 bp
  55% of sequence in fragments shorter than    3869 bp
  60% of sequence in fragments shorter than    4342 bp
  65% of sequence in fragments shorter than    4897 bp
  70% of sequence in fragments shorter than    5561 bp
  75% of sequence in fragments shorter than    6383 bp
  80% of sequence in fragments shorter than    7467 bp
  85% of sequence in fragments shorter than    8998 bp
  90% of sequence in fragments shorter than   11719 bp
  95% of sequence in fragments shorter than   18585 bp

# BstEII.PstI (GGTNACC+CTGCAG) double digest, 1656 thousand fragments (335865+1321469 sites), 1798 bp average fragment length
   5% of sequence in fragments shorter than     602 bp
  10% of sequence in fragments shorter than     925 bp
  15% of sequence in fragments shorter than    1229 bp
  20% of sequence in fragments shorter than    1522 bp
  25% of sequence in fragments shorter than    1817 bp
  30% of sequence in fragments shorter than    2110 bp
  35% of sequence in fragments shorter than    2424 bp
  40% of sequence in fragments shorter than    2753 bp
  45% of sequence in fragments shorter than    3096 bp
  50% of sequence in fragments shorter than    3469 bp
  55% of sequence in fragments shorter than    3878 bp
  60% of sequence in fragments shorter than    4328 bp
  65% of sequence in fragments shorter than    4829 bp
  70% of sequence in fragments shorter than    5393 bp
  75% of sequence in fragments shorter than    6056 bp
  80% of sequence in fragments shorter than    6878 bp
  85% of sequence in fragments shorter than    7918 bp
  90% of sequence in fragments shorter than    9458 bp
  95% of sequence in fragments shorter than   12292 bp

# HgaI.PpuMI (GACGC+RGGWCCY) double digest, 1656 thousand fragments (571889+1085138 sites), 1798 bp average fragment length
   5% of sequence in fragments shorter than     567 bp
  10% of sequence in fragments shorter than     947 bp
  15% of sequence in fragments shorter than    1298 bp
  20% of sequence in fragments shorter than    1642 bp
  25% of sequence in fragments shorter than    1990 bp
  30% of sequence in fragments shorter than    2344 bp
  35% of sequence in fragments shorter than    2715 bp
  40% of sequence in fragments shorter than    3107 bp
  45% of sequence in fragments shorter than    3518 bp
  50% of sequence in fragments shorter than    3948 bp
  55% of sequence in fragments shorter than    4436 bp
  60% of sequence in fragments shorter than    4979 bp
  65% of sequence in fragments shorter than    5588 bp
  70% of sequence in fragments shorter than    6286 bp
  75% of sequence in fragments shorter than    7126 bp
  80% of sequence in fragments shorter than    8158 bp
  85% of sequence in fragments shorter than    9488 bp
  90% of sequence in fragments shorter than   11430 bp
  95% of sequence in fragments shorter than   15155 bp

# AgeI.EsaBC3I (ACCGGT+TCGA) double digest, 1656 thousand fragments (53386+1603346 sites), 1799 bp average fragment length
   5% of sequence in fragments shorter than     614 bp
  10% of sequence in fragments shorter than     941 bp
  15% of sequence in fragments shorter than    1231 bp
  20% of sequence in fragments shorter than    1499 bp
  25% of sequence in fragments shorter than    1774 bp
  30% of sequence in fragments shorter than    2055 bp
  35% of sequence in fragments shorter than    2344 bp
  40% of sequence in fragments shorter than    2643 bp
  45% of sequence in fragments shorter than    2955 bp
  50% of sequence in fragments shorter than    3288 bp
  55% of sequence in fragments shorter than    3649 bp
  60% of sequence in fragments shorter than    4044 bp
  65% of sequence in fragments shorter than    4489 bp
  70% of sequence in fragments shorter than    4988 bp
  75% of sequence in fragments shorter than    5581 bp
  80% of sequence in fragments shorter than    6294 bp
  85% of sequence in fragments shorter than    7218 bp
  90% of sequence in fragments shorter than    8481 bp
  95% of sequence in fragments shorter than   10676 bp

# EciI.PpuMI (GGCGGA+RGGWCCY) double digest, 1656 thousand fragments (571273+1085138 sites), 1799 bp average fragment length
   5% of sequence in fragments shorter than     570 bp
  10% of sequence in fragments shorter than     923 bp
  15% of sequence in fragments shorter than    1243 bp
  20% of sequence in fragments shorter than    1560 bp
  25% of sequence in fragments shorter than    1879 bp
  30% of sequence in fragments shorter than    2204 bp
  35% of sequence in fragments shorter than    2545 bp
  40% of sequence in fragments shorter than    2908 bp
  45% of sequence in fragments shorter than    3283 bp
  50% of sequence in fragments shorter than    3666 bp
  55% of sequence in fragments shorter than    4112 bp
  60% of sequence in fragments shorter than    4623 bp
  65% of sequence in fragments shorter than    5202 bp
  70% of sequence in fragments shorter than    5873 bp
  75% of sequence in fragments shorter than    6668 bp
  80% of sequence in fragments shorter than    7659 bp
  85% of sequence in fragments shorter than    8971 bp
  90% of sequence in fragments shorter than   10972 bp
  95% of sequence in fragments shorter than   15067 bp

# HgaI.PvuII (GACGC+CAGCTG) double digest, 1655 thousand fragments (571889+1084260 sites), 1799 bp average fragment length
   5% of sequence in fragments shorter than     585 bp
  10% of sequence in fragments shorter than     943 bp
  15% of sequence in fragments shorter than    1274 bp
  20% of sequence in fragments shorter than    1591 bp
  25% of sequence in fragments shorter than    1914 bp
  30% of sequence in fragments shorter than    2241 bp
  35% of sequence in fragments shorter than    2583 bp
  40% of sequence in fragments shorter than    2936 bp
  45% of sequence in fragments shorter than    3322 bp
  50% of sequence in fragments shorter than    3735 bp
  55% of sequence in fragments shorter than    4186 bp
  60% of sequence in fragments shorter than    4690 bp
  65% of sequence in fragments shorter than    5259 bp
  70% of sequence in fragments shorter than    5904 bp
  75% of sequence in fragments shorter than    6661 bp
  80% of sequence in fragments shorter than    7579 bp
  85% of sequence in fragments shorter than    8778 bp
  90% of sequence in fragments shorter than   10574 bp
  95% of sequence in fragments shorter than   13883 bp

# EciI.PvuII (GGCGGA+CAGCTG) double digest, 1655 thousand fragments (571273+1084260 sites), 1800 bp average fragment length
   5% of sequence in fragments shorter than     586 bp
  10% of sequence in fragments shorter than     926 bp
  15% of sequence in fragments shorter than    1232 bp
  20% of sequence in fragments shorter than    1530 bp
  25% of sequence in fragments shorter than    1832 bp
  30% of sequence in fragments shorter than    2143 bp
  35% of sequence in fragments shorter than    2462 bp
  40% of sequence in fragments shorter than    2800 bp
  45% of sequence in fragments shorter than    3165 bp
  50% of sequence in fragments shorter than    3565 bp
  55% of sequence in fragments shorter than    4009 bp
  60% of sequence in fragments shorter than    4504 bp
  65% of sequence in fragments shorter than    5069 bp
  70% of sequence in fragments shorter than    5722 bp
  75% of sequence in fragments shorter than    6480 bp
  80% of sequence in fragments shorter than    7405 bp
  85% of sequence in fragments shorter than    8646 bp
  90% of sequence in fragments shorter than   10513 bp
  95% of sequence in fragments shorter than   14192 bp

# AscI.PsiI (GGCGCGCC+TTATAA) double digest, 1652 thousand fragments (4751+1647912 sites), 1803 bp average fragment length
   5% of sequence in fragments shorter than     600 bp
  10% of sequence in fragments shorter than     938 bp
  15% of sequence in fragments shorter than    1239 bp
  20% of sequence in fragments shorter than    1527 bp
  25% of sequence in fragments shorter than    1818 bp
  30% of sequence in fragments shorter than    2114 bp
  35% of sequence in fragments shorter than    2427 bp
  40% of sequence in fragments shorter than    2758 bp
  45% of sequence in fragments shorter than    3091 bp
  50% of sequence in fragments shorter than    3469 bp
  55% of sequence in fragments shorter than    3890 bp
  60% of sequence in fragments shorter than    4367 bp
  65% of sequence in fragments shorter than    4929 bp
  70% of sequence in fragments shorter than    5603 bp
  75% of sequence in fragments shorter than    6438 bp
  80% of sequence in fragments shorter than    7546 bp
  85% of sequence in fragments shorter than    9135 bp
  90% of sequence in fragments shorter than   11966 bp
  95% of sequence in fragments shorter than   19297 bp

# AccI.SphI (GTMKAC+GCATGC) double digest, 1651 thousand fragments (1101063+550952 sites), 1804 bp average fragment length
   5% of sequence in fragments shorter than     636 bp
  10% of sequence in fragments shorter than     963 bp
  15% of sequence in fragments shorter than    1241 bp
  20% of sequence in fragments shorter than    1500 bp
  25% of sequence in fragments shorter than    1755 bp
  30% of sequence in fragments shorter than    2006 bp
  35% of sequence in fragments shorter than    2265 bp
  40% of sequence in fragments shorter than    2533 bp
  45% of sequence in fragments shorter than    2808 bp
  50% of sequence in fragments shorter than    3103 bp
  55% of sequence in fragments shorter than    3422 bp
  60% of sequence in fragments shorter than    3762 bp
  65% of sequence in fragments shorter than    4146 bp
  70% of sequence in fragments shorter than    4577 bp
  75% of sequence in fragments shorter than    5071 bp
  80% of sequence in fragments shorter than    5647 bp
  85% of sequence in fragments shorter than    6346 bp
  90% of sequence in fragments shorter than    7400 bp
  95% of sequence in fragments shorter than    9340 bp

# MfeI.PpuMI (CAATTG+RGGWCCY) double digest, 1648 thousand fragments (564303+1085138 sites), 1807 bp average fragment length
   5% of sequence in fragments shorter than     618 bp
  10% of sequence in fragments shorter than     957 bp
  15% of sequence in fragments shorter than    1259 bp
  20% of sequence in fragments shorter than    1552 bp
  25% of sequence in fragments shorter than    1836 bp
  30% of sequence in fragments shorter than    2111 bp
  35% of sequence in fragments shorter than    2402 bp
  40% of sequence in fragments shorter than    2703 bp
  45% of sequence in fragments shorter than    3021 bp
  50% of sequence in fragments shorter than    3352 bp
  55% of sequence in fragments shorter than    3683 bp
  60% of sequence in fragments shorter than    4070 bp
  65% of sequence in fragments shorter than    4504 bp
  70% of sequence in fragments shorter than    5009 bp
  75% of sequence in fragments shorter than    5600 bp
  80% of sequence in fragments shorter than    6318 bp
  85% of sequence in fragments shorter than    7269 bp
  90% of sequence in fragments shorter than    8621 bp
  95% of sequence in fragments shorter than   11226 bp

# AflII.AvaI (CTTAAG+CYCGRG) double digest, 1648 thousand fragments (643284+1005714 sites), 1807 bp average fragment length
   5% of sequence in fragments shorter than     613 bp
  10% of sequence in fragments shorter than     959 bp
  15% of sequence in fragments shorter than    1269 bp
  20% of sequence in fragments shorter than    1565 bp
  25% of sequence in fragments shorter than    1859 bp
  30% of sequence in fragments shorter than    2153 bp
  35% of sequence in fragments shorter than    2456 bp
  40% of sequence in fragments shorter than    2754 bp
  45% of sequence in fragments shorter than    3084 bp
  50% of sequence in fragments shorter than    3448 bp
  55% of sequence in fragments shorter than    3833 bp
  60% of sequence in fragments shorter than    4260 bp
  65% of sequence in fragments shorter than    4738 bp
  70% of sequence in fragments shorter than    5297 bp
  75% of sequence in fragments shorter than    5946 bp
  80% of sequence in fragments shorter than    6754 bp
  85% of sequence in fragments shorter than    7800 bp
  90% of sequence in fragments shorter than    9364 bp
  95% of sequence in fragments shorter than   12656 bp

# MfeI.PvuII (CAATTG+CAGCTG) double digest, 1648 thousand fragments (564303+1084260 sites), 1808 bp average fragment length
   5% of sequence in fragments shorter than     634 bp
  10% of sequence in fragments shorter than     957 bp
  15% of sequence in fragments shorter than    1245 bp
  20% of sequence in fragments shorter than    1518 bp
  25% of sequence in fragments shorter than    1787 bp
  30% of sequence in fragments shorter than    2050 bp
  35% of sequence in fragments shorter than    2315 bp
  40% of sequence in fragments shorter than    2591 bp
  45% of sequence in fragments shorter than    2872 bp
  50% of sequence in fragments shorter than    3177 bp
  55% of sequence in fragments shorter than    3508 bp
  60% of sequence in fragments shorter than    3849 bp
  65% of sequence in fragments shorter than    4249 bp
  70% of sequence in fragments shorter than    4704 bp
  75% of sequence in fragments shorter than    5235 bp
  80% of sequence in fragments shorter than    5883 bp
  85% of sequence in fragments shorter than    6707 bp
  90% of sequence in fragments shorter than    7888 bp
  95% of sequence in fragments shorter than   10056 bp

# AvaIII.BsaAI (ATGCAT+YACGTR) double digest, 1646 thousand fragments (933357+713801 sites), 1809 bp average fragment length
   5% of sequence in fragments shorter than     653 bp
  10% of sequence in fragments shorter than     988 bp
  15% of sequence in fragments shorter than    1275 bp
  20% of sequence in fragments shorter than    1545 bp
  25% of sequence in fragments shorter than    1810 bp
  30% of sequence in fragments shorter than    2069 bp
  35% of sequence in fragments shorter than    2337 bp
  40% of sequence in fragments shorter than    2614 bp
  45% of sequence in fragments shorter than    2899 bp
  50% of sequence in fragments shorter than    3201 bp
  55% of sequence in fragments shorter than    3519 bp
  60% of sequence in fragments shorter than    3880 bp
  65% of sequence in fragments shorter than    4280 bp
  70% of sequence in fragments shorter than    4723 bp
  75% of sequence in fragments shorter than    5250 bp
  80% of sequence in fragments shorter than    5881 bp
  85% of sequence in fragments shorter than    6688 bp
  90% of sequence in fragments shorter than    7854 bp
  95% of sequence in fragments shorter than    9939 bp

# EsaBC3I.PmeI (TCGA+GTTTAAAC) double digest, 1644 thousand fragments (1603346+42094 sites), 1811 bp average fragment length
   5% of sequence in fragments shorter than     620 bp
  10% of sequence in fragments shorter than     947 bp
  15% of sequence in fragments shorter than    1238 bp
  20% of sequence in fragments shorter than    1508 bp
  25% of sequence in fragments shorter than    1783 bp
  30% of sequence in fragments shorter than    2064 bp
  35% of sequence in fragments shorter than    2350 bp
  40% of sequence in fragments shorter than    2648 bp
  45% of sequence in fragments shorter than    2957 bp
  50% of sequence in fragments shorter than    3288 bp
  55% of sequence in fragments shorter than    3647 bp
  60% of sequence in fragments shorter than    4040 bp
  65% of sequence in fragments shorter than    4481 bp
  70% of sequence in fragments shorter than    4973 bp
  75% of sequence in fragments shorter than    5561 bp
  80% of sequence in fragments shorter than    6263 bp
  85% of sequence in fragments shorter than    7173 bp
  90% of sequence in fragments shorter than    8421 bp
  95% of sequence in fragments shorter than   10586 bp

# AflIII.PacI (ACRYGT+TTAATTAA) double digest, 1644 thousand fragments (1485394+159455 sites), 1812 bp average fragment length
   5% of sequence in fragments shorter than     653 bp
  10% of sequence in fragments shorter than    1015 bp
  15% of sequence in fragments shorter than    1327 bp
  20% of sequence in fragments shorter than    1620 bp
  25% of sequence in fragments shorter than    1903 bp
  30% of sequence in fragments shorter than    2179 bp
  35% of sequence in fragments shorter than    2465 bp
  40% of sequence in fragments shorter than    2759 bp
  45% of sequence in fragments shorter than    3066 bp
  50% of sequence in fragments shorter than    3394 bp
  55% of sequence in fragments shorter than    3751 bp
  60% of sequence in fragments shorter than    4141 bp
  65% of sequence in fragments shorter than    4574 bp
  70% of sequence in fragments shorter than    5072 bp
  75% of sequence in fragments shorter than    5654 bp
  80% of sequence in fragments shorter than    6331 bp
  85% of sequence in fragments shorter than    7198 bp
  90% of sequence in fragments shorter than    8466 bp
  95% of sequence in fragments shorter than   10866 bp

# AccI.ScaI (GTMKAC+AGTACT) double digest, 1644 thousand fragments (1101063+543793 sites), 1812 bp average fragment length
   5% of sequence in fragments shorter than     633 bp
  10% of sequence in fragments shorter than     961 bp
  15% of sequence in fragments shorter than    1241 bp
  20% of sequence in fragments shorter than    1502 bp
  25% of sequence in fragments shorter than    1760 bp
  30% of sequence in fragments shorter than    2020 bp
  35% of sequence in fragments shorter than    2284 bp
  40% of sequence in fragments shorter than    2559 bp
  45% of sequence in fragments shorter than    2844 bp
  50% of sequence in fragments shorter than    3153 bp
  55% of sequence in fragments shorter than    3489 bp
  60% of sequence in fragments shorter than    3853 bp
  65% of sequence in fragments shorter than    4264 bp
  70% of sequence in fragments shorter than    4731 bp
  75% of sequence in fragments shorter than    5280 bp
  80% of sequence in fragments shorter than    5931 bp
  85% of sequence in fragments shorter than    6724 bp
  90% of sequence in fragments shorter than    7977 bp
  95% of sequence in fragments shorter than   10369 bp

# AflIII.Hpy99I (ACRYGT+CGWCG) double digest, 1642 thousand fragments (1485394+157917 sites), 1813 bp average fragment length
   5% of sequence in fragments shorter than     653 bp
  10% of sequence in fragments shorter than    1021 bp
  15% of sequence in fragments shorter than    1337 bp
  20% of sequence in fragments shorter than    1635 bp
  25% of sequence in fragments shorter than    1919 bp
  30% of sequence in fragments shorter than    2199 bp
  35% of sequence in fragments shorter than    2488 bp
  40% of sequence in fragments shorter than    2782 bp
  45% of sequence in fragments shorter than    3090 bp
  50% of sequence in fragments shorter than    3417 bp
  55% of sequence in fragments shorter than    3771 bp
  60% of sequence in fragments shorter than    4156 bp
  65% of sequence in fragments shorter than    4584 bp
  70% of sequence in fragments shorter than    5064 bp
  75% of sequence in fragments shorter than    5606 bp
  80% of sequence in fragments shorter than    6260 bp
  85% of sequence in fragments shorter than    7105 bp
  90% of sequence in fragments shorter than    8323 bp
  95% of sequence in fragments shorter than   10523 bp

# BclI.NdeI (TGATCA+CATATG) double digest, 1641 thousand fragments (738785+903360 sites), 1815 bp average fragment length
   5% of sequence in fragments shorter than     631 bp
  10% of sequence in fragments shorter than     941 bp
  15% of sequence in fragments shorter than    1215 bp
  20% of sequence in fragments shorter than    1495 bp
  25% of sequence in fragments shorter than    1767 bp
  30% of sequence in fragments shorter than    2039 bp
  35% of sequence in fragments shorter than    2321 bp
  40% of sequence in fragments shorter than    2611 bp
  45% of sequence in fragments shorter than    2914 bp
  50% of sequence in fragments shorter than    3241 bp
  55% of sequence in fragments shorter than    3596 bp
  60% of sequence in fragments shorter than    3981 bp
  65% of sequence in fragments shorter than    4410 bp
  70% of sequence in fragments shorter than    4901 bp
  75% of sequence in fragments shorter than    5475 bp
  80% of sequence in fragments shorter than    6208 bp
  85% of sequence in fragments shorter than    7173 bp
  90% of sequence in fragments shorter than    8651 bp
  95% of sequence in fragments shorter than   11874 bp

# BsaAI.StuI (YACGTR+AGGCCT) double digest, 1639 thousand fragments (713801+925729 sites), 1818 bp average fragment length
   5% of sequence in fragments shorter than     598 bp
  10% of sequence in fragments shorter than     915 bp
  15% of sequence in fragments shorter than    1244 bp
  20% of sequence in fragments shorter than    1554 bp
  25% of sequence in fragments shorter than    1845 bp
  30% of sequence in fragments shorter than    2140 bp
  35% of sequence in fragments shorter than    2458 bp
  40% of sequence in fragments shorter than    2785 bp
  45% of sequence in fragments shorter than    3126 bp
  50% of sequence in fragments shorter than    3485 bp
  55% of sequence in fragments shorter than    3864 bp
  60% of sequence in fragments shorter than    4285 bp
  65% of sequence in fragments shorter than    4752 bp
  70% of sequence in fragments shorter than    5285 bp
  75% of sequence in fragments shorter than    5893 bp
  80% of sequence in fragments shorter than    6627 bp
  85% of sequence in fragments shorter than    7554 bp
  90% of sequence in fragments shorter than    8839 bp
  95% of sequence in fragments shorter than   11064 bp

# AvaIII.BceAI (ATGCAT+ACGGC) double digest, 1638 thousand fragments (933357+705178 sites), 1819 bp average fragment length
   5% of sequence in fragments shorter than     634 bp
  10% of sequence in fragments shorter than     977 bp
  15% of sequence in fragments shorter than    1276 bp
  20% of sequence in fragments shorter than    1556 bp
  25% of sequence in fragments shorter than    1833 bp
  30% of sequence in fragments shorter than    2101 bp
  35% of sequence in fragments shorter than    2384 bp
  40% of sequence in fragments shorter than    2673 bp
  45% of sequence in fragments shorter than    2975 bp
  50% of sequence in fragments shorter than    3302 bp
  55% of sequence in fragments shorter than    3653 bp
  60% of sequence in fragments shorter than    4038 bp
  65% of sequence in fragments shorter than    4462 bp
  70% of sequence in fragments shorter than    4950 bp
  75% of sequence in fragments shorter than    5510 bp
  80% of sequence in fragments shorter than    6181 bp
  85% of sequence in fragments shorter than    7042 bp
  90% of sequence in fragments shorter than    8257 bp
  95% of sequence in fragments shorter than   10483 bp

# ClaI.HgiCI (ATCGAT+GGYRCC) double digest, 1636 thousand fragments (86477+1550877 sites), 1820 bp average fragment length
   5% of sequence in fragments shorter than     601 bp
  10% of sequence in fragments shorter than     941 bp
  15% of sequence in fragments shorter than    1227 bp
  20% of sequence in fragments shorter than    1520 bp
  25% of sequence in fragments shorter than    1802 bp
  30% of sequence in fragments shorter than    2106 bp
  35% of sequence in fragments shorter than    2419 bp
  40% of sequence in fragments shorter than    2746 bp
  45% of sequence in fragments shorter than    3078 bp
  50% of sequence in fragments shorter than    3449 bp
  55% of sequence in fragments shorter than    3855 bp
  60% of sequence in fragments shorter than    4306 bp
  65% of sequence in fragments shorter than    4814 bp
  70% of sequence in fragments shorter than    5409 bp
  75% of sequence in fragments shorter than    6095 bp
  80% of sequence in fragments shorter than    6969 bp
  85% of sequence in fragments shorter than    8110 bp
  90% of sequence in fragments shorter than    9808 bp
  95% of sequence in fragments shorter than   13286 bp

# FnuDII.NdeI (CGCG+CATATG) double digest, 1636 thousand fragments (733940+903360 sites), 1820 bp average fragment length
   5% of sequence in fragments shorter than     691 bp
  10% of sequence in fragments shorter than    1098 bp
  15% of sequence in fragments shorter than    1446 bp
  20% of sequence in fragments shorter than    1788 bp
  25% of sequence in fragments shorter than    2125 bp
  30% of sequence in fragments shorter than    2459 bp
  35% of sequence in fragments shorter than    2801 bp
  40% of sequence in fragments shorter than    3150 bp
  45% of sequence in fragments shorter than    3514 bp
  50% of sequence in fragments shorter than    3900 bp
  55% of sequence in fragments shorter than    4315 bp
  60% of sequence in fragments shorter than    4742 bp
  65% of sequence in fragments shorter than    5233 bp
  70% of sequence in fragments shorter than    5787 bp
  75% of sequence in fragments shorter than    6436 bp
  80% of sequence in fragments shorter than    7221 bp
  85% of sequence in fragments shorter than    8240 bp
  90% of sequence in fragments shorter than    9713 bp
  95% of sequence in fragments shorter than   12545 bp

# PpuMI.SphI (RGGWCCY+GCATGC) double digest, 1635 thousand fragments (1085138+550952 sites), 1821 bp average fragment length
   5% of sequence in fragments shorter than     602 bp
  10% of sequence in fragments shorter than     949 bp
  15% of sequence in fragments shorter than    1259 bp
  20% of sequence in fragments shorter than    1558 bp
  25% of sequence in fragments shorter than    1857 bp
  30% of sequence in fragments shorter than    2156 bp
  35% of sequence in fragments shorter than    2468 bp
  40% of sequence in fragments shorter than    2797 bp
  45% of sequence in fragments shorter than    3142 bp
  50% of sequence in fragments shorter than    3505 bp
  55% of sequence in fragments shorter than    3871 bp
  60% of sequence in fragments shorter than    4315 bp
  65% of sequence in fragments shorter than    4816 bp
  70% of sequence in fragments shorter than    5386 bp
  75% of sequence in fragments shorter than    6059 bp
  80% of sequence in fragments shorter than    6890 bp
  85% of sequence in fragments shorter than    7954 bp
  90% of sequence in fragments shorter than    9555 bp
  95% of sequence in fragments shorter than   12718 bp

# AvaI.HaeII (CYCGRG+RGCGCY) double digest, 1635 thousand fragments (1005714+630101 sites), 1822 bp average fragment length
   5% of sequence in fragments shorter than     584 bp
  10% of sequence in fragments shorter than     988 bp
  15% of sequence in fragments shorter than    1395 bp
  20% of sequence in fragments shorter than    1825 bp
  25% of sequence in fragments shorter than    2261 bp
  30% of sequence in fragments shorter than    2742 bp
  35% of sequence in fragments shorter than    3257 bp
  40% of sequence in fragments shorter than    3813 bp
  45% of sequence in fragments shorter than    4421 bp
  50% of sequence in fragments shorter than    5094 bp
  55% of sequence in fragments shorter than    5831 bp
  60% of sequence in fragments shorter than    6666 bp
  65% of sequence in fragments shorter than    7605 bp
  70% of sequence in fragments shorter than    8698 bp
  75% of sequence in fragments shorter than   10008 bp
  80% of sequence in fragments shorter than   11617 bp
  85% of sequence in fragments shorter than   13735 bp
  90% of sequence in fragments shorter than   16649 bp
  95% of sequence in fragments shorter than   21728 bp

# BsmI.ClaI (GAATGC+ATCGAT) double digest, 1635 thousand fragments (1549350+86477 sites), 1822 bp average fragment length
   5% of sequence in fragments shorter than     638 bp
  10% of sequence in fragments shorter than    1091 bp
  15% of sequence in fragments shorter than    1464 bp
  20% of sequence in fragments shorter than    1807 bp
  25% of sequence in fragments shorter than    2131 bp
  30% of sequence in fragments shorter than    2454 bp
  35% of sequence in fragments shorter than    2770 bp
  40% of sequence in fragments shorter than    3105 bp
  45% of sequence in fragments shorter than    3456 bp
  50% of sequence in fragments shorter than    3824 bp
  55% of sequence in fragments shorter than    4219 bp
  60% of sequence in fragments shorter than    4647 bp
  65% of sequence in fragments shorter than    5119 bp
  70% of sequence in fragments shorter than    5651 bp
  75% of sequence in fragments shorter than    6262 bp
  80% of sequence in fragments shorter than    6994 bp
  85% of sequence in fragments shorter than    7921 bp
  90% of sequence in fragments shorter than    9206 bp
  95% of sequence in fragments shorter than   11350 bp

# PvuII.SphI (CAGCTG+GCATGC) double digest, 1634 thousand fragments (1084260+550952 sites), 1822 bp average fragment length
   5% of sequence in fragments shorter than     617 bp
  10% of sequence in fragments shorter than     953 bp
  15% of sequence in fragments shorter than    1251 bp
  20% of sequence in fragments shorter than    1534 bp
  25% of sequence in fragments shorter than    1817 bp
  30% of sequence in fragments shorter than    2106 bp
  35% of sequence in fragments shorter than    2403 bp
  40% of sequence in fragments shorter than    2713 bp
  45% of sequence in fragments shorter than    3035 bp
  50% of sequence in fragments shorter than    3387 bp
  55% of sequence in fragments shorter than    3770 bp
  60% of sequence in fragments shorter than    4201 bp
  65% of sequence in fragments shorter than    4681 bp
  70% of sequence in fragments shorter than    5237 bp
  75% of sequence in fragments shorter than    5876 bp
  80% of sequence in fragments shorter than    6658 bp
  85% of sequence in fragments shorter than    7659 bp
  90% of sequence in fragments shorter than    9154 bp
  95% of sequence in fragments shorter than   12057 bp

# BglII.HindIII (AGATCT+AAGCTT) double digest, 1634 thousand fragments (774732+860365 sites), 1823 bp average fragment length
   5% of sequence in fragments shorter than     630 bp
  10% of sequence in fragments shorter than     953 bp
  15% of sequence in fragments shorter than    1230 bp
  20% of sequence in fragments shorter than    1493 bp
  25% of sequence in fragments shorter than    1751 bp
  30% of sequence in fragments shorter than    2011 bp
  35% of sequence in fragments shorter than    2282 bp
  40% of sequence in fragments shorter than    2560 bp
  45% of sequence in fragments shorter than    2845 bp
  50% of sequence in fragments shorter than    3147 bp
  55% of sequence in fragments shorter than    3477 bp
  60% of sequence in fragments shorter than    3832 bp
  65% of sequence in fragments shorter than    4232 bp
  70% of sequence in fragments shorter than    4685 bp
  75% of sequence in fragments shorter than    5215 bp
  80% of sequence in fragments shorter than    5868 bp
  85% of sequence in fragments shorter than    6714 bp
  90% of sequence in fragments shorter than    7964 bp
  95% of sequence in fragments shorter than   10406 bp

# EsaBC3I.SalI (TCGA+GTCGAC) double digest, 1634 thousand fragments (1603346+31396 sites), 1823 bp average fragment length
   5% of sequence in fragments shorter than     635 bp
  10% of sequence in fragments shorter than     972 bp
  15% of sequence in fragments shorter than    1272 bp
  20% of sequence in fragments shorter than    1551 bp
  25% of sequence in fragments shorter than    1834 bp
  30% of sequence in fragments shorter than    2123 bp
  35% of sequence in fragments shorter than    2421 bp
  40% of sequence in fragments shorter than    2730 bp
  45% of sequence in fragments shorter than    3051 bp
  50% of sequence in fragments shorter than    3392 bp
  55% of sequence in fragments shorter than    3765 bp
  60% of sequence in fragments shorter than    4172 bp
  65% of sequence in fragments shorter than    4630 bp
  70% of sequence in fragments shorter than    5147 bp
  75% of sequence in fragments shorter than    5757 bp
  80% of sequence in fragments shorter than    6494 bp
  85% of sequence in fragments shorter than    7439 bp
  90% of sequence in fragments shorter than    8738 bp
  95% of sequence in fragments shorter than   11017 bp

# BscGI.SpeI (CCCGT+ACTAGT) double digest, 1630 thousand fragments (1231070+400286 sites), 1827 bp average fragment length
   5% of sequence in fragments shorter than     555 bp
  10% of sequence in fragments shorter than     927 bp
  15% of sequence in fragments shorter than    1265 bp
  20% of sequence in fragments shorter than    1586 bp
  25% of sequence in fragments shorter than    1905 bp
  30% of sequence in fragments shorter than    2226 bp
  35% of sequence in fragments shorter than    2554 bp
  40% of sequence in fragments shorter than    2895 bp
  45% of sequence in fragments shorter than    3252 bp
  50% of sequence in fragments shorter than    3629 bp
  55% of sequence in fragments shorter than    4039 bp
  60% of sequence in fragments shorter than    4460 bp
  65% of sequence in fragments shorter than    4946 bp
  70% of sequence in fragments shorter than    5499 bp
  75% of sequence in fragments shorter than    6151 bp
  80% of sequence in fragments shorter than    6939 bp
  85% of sequence in fragments shorter than    7944 bp
  90% of sequence in fragments shorter than    9361 bp
  95% of sequence in fragments shorter than   11773 bp

# BceAI.StuI (ACGGC+AGGCCT) double digest, 1630 thousand fragments (705178+925729 sites), 1827 bp average fragment length
   5% of sequence in fragments shorter than     565 bp
  10% of sequence in fragments shorter than     911 bp
  15% of sequence in fragments shorter than    1258 bp
  20% of sequence in fragments shorter than    1591 bp
  25% of sequence in fragments shorter than    1940 bp
  30% of sequence in fragments shorter than    2274 bp
  35% of sequence in fragments shorter than    2644 bp
  40% of sequence in fragments shorter than    3029 bp
  45% of sequence in fragments shorter than    3435 bp
  50% of sequence in fragments shorter than    3871 bp
  55% of sequence in fragments shorter than    4342 bp
  60% of sequence in fragments shorter than    4861 bp
  65% of sequence in fragments shorter than    5436 bp
  70% of sequence in fragments shorter than    6084 bp
  75% of sequence in fragments shorter than    6848 bp
  80% of sequence in fragments shorter than    7779 bp
  85% of sequence in fragments shorter than    8955 bp
  90% of sequence in fragments shorter than   10573 bp
  95% of sequence in fragments shorter than   13399 bp

# AvaIII.BciVI (ATGCAT+GTATCC) double digest, 1628 thousand fragments (933357+696093 sites), 1829 bp average fragment length
   5% of sequence in fragments shorter than     649 bp
  10% of sequence in fragments shorter than     984 bp
  15% of sequence in fragments shorter than    1278 bp
  20% of sequence in fragments shorter than    1549 bp
  25% of sequence in fragments shorter than    1811 bp
  30% of sequence in fragments shorter than    2067 bp
  35% of sequence in fragments shorter than    2336 bp
  40% of sequence in fragments shorter than    2616 bp
  45% of sequence in fragments shorter than    2907 bp
  50% of sequence in fragments shorter than    3219 bp
  55% of sequence in fragments shorter than    3554 bp
  60% of sequence in fragments shorter than    3927 bp
  65% of sequence in fragments shorter than    4337 bp
  70% of sequence in fragments shorter than    4806 bp
  75% of sequence in fragments shorter than    5351 bp
  80% of sequence in fragments shorter than    6025 bp
  85% of sequence in fragments shorter than    6908 bp
  90% of sequence in fragments shorter than    8201 bp
  95% of sequence in fragments shorter than   10752 bp

# PpuMI.ScaI (RGGWCCY+AGTACT) double digest, 1628 thousand fragments (1085138+543793 sites), 1829 bp average fragment length
   5% of sequence in fragments shorter than     621 bp
  10% of sequence in fragments shorter than     968 bp
  15% of sequence in fragments shorter than    1272 bp
  20% of sequence in fragments shorter than    1561 bp
  25% of sequence in fragments shorter than    1845 bp
  30% of sequence in fragments shorter than    2124 bp
  35% of sequence in fragments shorter than    2414 bp
  40% of sequence in fragments shorter than    2714 bp
  45% of sequence in fragments shorter than    3033 bp
  50% of sequence in fragments shorter than    3363 bp
  55% of sequence in fragments shorter than    3694 bp
  60% of sequence in fragments shorter than    4081 bp
  65% of sequence in fragments shorter than    4519 bp
  70% of sequence in fragments shorter than    5024 bp
  75% of sequence in fragments shorter than    5618 bp
  80% of sequence in fragments shorter than    6334 bp
  85% of sequence in fragments shorter than    7284 bp
  90% of sequence in fragments shorter than    8660 bp
  95% of sequence in fragments shorter than   11449 bp

# PvuII.ScaI (CAGCTG+AGTACT) double digest, 1627 thousand fragments (1084260+543793 sites), 1830 bp average fragment length
   5% of sequence in fragments shorter than     633 bp
  10% of sequence in fragments shorter than     968 bp
  15% of sequence in fragments shorter than    1260 bp
  20% of sequence in fragments shorter than    1534 bp
  25% of sequence in fragments shorter than    1803 bp
  30% of sequence in fragments shorter than    2075 bp
  35% of sequence in fragments shorter than    2355 bp
  40% of sequence in fragments shorter than    2642 bp
  45% of sequence in fragments shorter than    2935 bp
  50% of sequence in fragments shorter than    3257 bp
  55% of sequence in fragments shorter than    3606 bp
  60% of sequence in fragments shorter than    3989 bp
  65% of sequence in fragments shorter than    4418 bp
  70% of sequence in fragments shorter than    4903 bp
  75% of sequence in fragments shorter than    5473 bp
  80% of sequence in fragments shorter than    6155 bp
  85% of sequence in fragments shorter than    7033 bp
  90% of sequence in fragments shorter than    8312 bp
  95% of sequence in fragments shorter than   10791 bp

# BglII.XbaI (AGATCT+TCTAGA) double digest, 1625 thousand fragments (774732+850998 sites), 1833 bp average fragment length
   5% of sequence in fragments shorter than     659 bp
  10% of sequence in fragments shorter than     971 bp
  15% of sequence in fragments shorter than    1253 bp
  20% of sequence in fragments shorter than    1510 bp
  25% of sequence in fragments shorter than    1764 bp
  30% of sequence in fragments shorter than    2028 bp
  35% of sequence in fragments shorter than    2291 bp
  40% of sequence in fragments shorter than    2571 bp
  45% of sequence in fragments shorter than    2862 bp
  50% of sequence in fragments shorter than    3176 bp
  55% of sequence in fragments shorter than    3512 bp
  60% of sequence in fragments shorter than    3880 bp
  65% of sequence in fragments shorter than    4290 bp
  70% of sequence in fragments shorter than    4757 bp
  75% of sequence in fragments shorter than    5298 bp
  80% of sequence in fragments shorter than    5962 bp
  85% of sequence in fragments shorter than    6821 bp
  90% of sequence in fragments shorter than    8058 bp
  95% of sequence in fragments shorter than   10327 bp

# EsaBC3I.MluI (TCGA+ACGCGT) double digest, 1624 thousand fragments (1603346+21415 sites), 1834 bp average fragment length
   5% of sequence in fragments shorter than     626 bp
  10% of sequence in fragments shorter than     959 bp
  15% of sequence in fragments shorter than    1256 bp
  20% of sequence in fragments shorter than    1530 bp
  25% of sequence in fragments shorter than    1812 bp
  30% of sequence in fragments shorter than    2098 bp
  35% of sequence in fragments shorter than    2393 bp
  40% of sequence in fragments shorter than    2699 bp
  45% of sequence in fragments shorter than    3016 bp
  50% of sequence in fragments shorter than    3356 bp
  55% of sequence in fragments shorter than    3723 bp
  60% of sequence in fragments shorter than    4127 bp
  65% of sequence in fragments shorter than    4582 bp
  70% of sequence in fragments shorter than    5093 bp
  75% of sequence in fragments shorter than    5700 bp
  80% of sequence in fragments shorter than    6432 bp
  85% of sequence in fragments shorter than    7369 bp
  90% of sequence in fragments shorter than    8660 bp
  95% of sequence in fragments shorter than   10929 bp

# BglII.EcoRI (AGATCT+GAATTC) double digest, 1621 thousand fragments (774732+847401 sites), 1837 bp average fragment length
   5% of sequence in fragments shorter than     627 bp
  10% of sequence in fragments shorter than     953 bp
  15% of sequence in fragments shorter than    1254 bp
  20% of sequence in fragments shorter than    1520 bp
  25% of sequence in fragments shorter than    1783 bp
  30% of sequence in fragments shorter than    2054 bp
  35% of sequence in fragments shorter than    2332 bp
  40% of sequence in fragments shorter than    2616 bp
  45% of sequence in fragments shorter than    2909 bp
  50% of sequence in fragments shorter than    3226 bp
  55% of sequence in fragments shorter than    3560 bp
  60% of sequence in fragments shorter than    3925 bp
  65% of sequence in fragments shorter than    4331 bp
  70% of sequence in fragments shorter than    4790 bp
  75% of sequence in fragments shorter than    5329 bp
  80% of sequence in fragments shorter than    5976 bp
  85% of sequence in fragments shorter than    6810 bp
  90% of sequence in fragments shorter than    7998 bp
  95% of sequence in fragments shorter than   10193 bp

# AatII.HgiCI (GACGTC+GGYRCC) double digest, 1621 thousand fragments (71089+1550877 sites), 1837 bp average fragment length
   5% of sequence in fragments shorter than     602 bp
  10% of sequence in fragments shorter than     949 bp
  15% of sequence in fragments shorter than    1239 bp
  20% of sequence in fragments shorter than    1540 bp
  25% of sequence in fragments shorter than    1832 bp
  30% of sequence in fragments shorter than    2146 bp
  35% of sequence in fragments shorter than    2472 bp
  40% of sequence in fragments shorter than    2803 bp
  45% of sequence in fragments shorter than    3149 bp
  50% of sequence in fragments shorter than    3540 bp
  55% of sequence in fragments shorter than    3965 bp
  60% of sequence in fragments shorter than    4430 bp
  65% of sequence in fragments shorter than    4967 bp
  70% of sequence in fragments shorter than    5584 bp
  75% of sequence in fragments shorter than    6312 bp
  80% of sequence in fragments shorter than    7236 bp
  85% of sequence in fragments shorter than    8442 bp
  90% of sequence in fragments shorter than   10225 bp
  95% of sequence in fragments shorter than   13977 bp

# BciVI.StuI (GTATCC+AGGCCT) double digest, 1621 thousand fragments (696093+925729 sites), 1837 bp average fragment length
   5% of sequence in fragments shorter than     602 bp
  10% of sequence in fragments shorter than     933 bp
  15% of sequence in fragments shorter than    1257 bp
  20% of sequence in fragments shorter than    1538 bp
  25% of sequence in fragments shorter than    1832 bp
  30% of sequence in fragments shorter than    2113 bp
  35% of sequence in fragments shorter than    2410 bp
  40% of sequence in fragments shorter than    2719 bp
  45% of sequence in fragments shorter than    3040 bp
  50% of sequence in fragments shorter than    3381 bp
  55% of sequence in fragments shorter than    3743 bp
  60% of sequence in fragments shorter than    4135 bp
  65% of sequence in fragments shorter than    4577 bp
  70% of sequence in fragments shorter than    5069 bp
  75% of sequence in fragments shorter than    5638 bp
  80% of sequence in fragments shorter than    6309 bp
  85% of sequence in fragments shorter than    7170 bp
  90% of sequence in fragments shorter than    8360 bp
  95% of sequence in fragments shorter than   10359 bp

# AflII.BspHI (CTTAAG+TCATGA) double digest, 1621 thousand fragments (643284+978290 sites), 1838 bp average fragment length
   5% of sequence in fragments shorter than     645 bp
  10% of sequence in fragments shorter than     975 bp
  15% of sequence in fragments shorter than    1263 bp
  20% of sequence in fragments shorter than    1536 bp
  25% of sequence in fragments shorter than    1801 bp
  30% of sequence in fragments shorter than    2060 bp
  35% of sequence in fragments shorter than    2315 bp
  40% of sequence in fragments shorter than    2588 bp
  45% of sequence in fragments shorter than    2878 bp
  50% of sequence in fragments shorter than    3186 bp
  55% of sequence in fragments shorter than    3520 bp
  60% of sequence in fragments shorter than    3880 bp
  65% of sequence in fragments shorter than    4287 bp
  70% of sequence in fragments shorter than    4741 bp
  75% of sequence in fragments shorter than    5277 bp
  80% of sequence in fragments shorter than    5948 bp
  85% of sequence in fragments shorter than    6825 bp
  90% of sequence in fragments shorter than    8136 bp
  95% of sequence in fragments shorter than   10906 bp

# BscGI.HpaI (CCCGT+GTTAAC) double digest, 1620 thousand fragments (1231070+389418 sites), 1839 bp average fragment length
   5% of sequence in fragments shorter than     554 bp
  10% of sequence in fragments shorter than     928 bp
  15% of sequence in fragments shorter than    1268 bp
  20% of sequence in fragments shorter than    1594 bp
  25% of sequence in fragments shorter than    1919 bp
  30% of sequence in fragments shorter than    2248 bp
  35% of sequence in fragments shorter than    2584 bp
  40% of sequence in fragments shorter than    2936 bp
  45% of sequence in fragments shorter than    3310 bp
  50% of sequence in fragments shorter than    3702 bp
  55% of sequence in fragments shorter than    4130 bp
  60% of sequence in fragments shorter than    4600 bp
  65% of sequence in fragments shorter than    5128 bp
  70% of sequence in fragments shorter than    5714 bp
  75% of sequence in fragments shorter than    6404 bp
  80% of sequence in fragments shorter than    7235 bp
  85% of sequence in fragments shorter than    8320 bp
  90% of sequence in fragments shorter than    9827 bp
  95% of sequence in fragments shorter than   12429 bp

# AatII.BsmI (GACGTC+GAATGC) double digest, 1619 thousand fragments (71089+1549350 sites), 1839 bp average fragment length
   5% of sequence in fragments shorter than     651 bp
  10% of sequence in fragments shorter than    1101 bp
  15% of sequence in fragments shorter than    1481 bp
  20% of sequence in fragments shorter than    1828 bp
  25% of sequence in fragments shorter than    2159 bp
  30% of sequence in fragments shorter than    2481 bp
  35% of sequence in fragments shorter than    2803 bp
  40% of sequence in fragments shorter than    3137 bp
  45% of sequence in fragments shorter than    3491 bp
  50% of sequence in fragments shorter than    3863 bp
  55% of sequence in fragments shorter than    4260 bp
  60% of sequence in fragments shorter than    4691 bp
  65% of sequence in fragments shorter than    5166 bp
  70% of sequence in fragments shorter than    5701 bp
  75% of sequence in fragments shorter than    6316 bp
  80% of sequence in fragments shorter than    7045 bp
  85% of sequence in fragments shorter than    7964 bp
  90% of sequence in fragments shorter than    9239 bp
  95% of sequence in fragments shorter than   11359 bp

# HindIII.NcoI (AAGCTT+CCATGG) double digest, 1619 thousand fragments (860365+759594 sites), 1840 bp average fragment length
   5% of sequence in fragments shorter than     640 bp
  10% of sequence in fragments shorter than     979 bp
  15% of sequence in fragments shorter than    1269 bp
  20% of sequence in fragments shorter than    1532 bp
  25% of sequence in fragments shorter than    1797 bp
  30% of sequence in fragments shorter than    2030 bp
  35% of sequence in fragments shorter than    2272 bp
  40% of sequence in fragments shorter than    2547 bp
  45% of sequence in fragments shorter than    2835 bp
  50% of sequence in fragments shorter than    3137 bp
  55% of sequence in fragments shorter than    3466 bp
  60% of sequence in fragments shorter than    3824 bp
  65% of sequence in fragments shorter than    4211 bp
  70% of sequence in fragments shorter than    4655 bp
  75% of sequence in fragments shorter than    5179 bp
  80% of sequence in fragments shorter than    5808 bp
  85% of sequence in fragments shorter than    6619 bp
  90% of sequence in fragments shorter than    7798 bp
  95% of sequence in fragments shorter than    9988 bp

# PstI.SexAI (CTGCAG+ACCWGGT) double digest, 1618 thousand fragments (1321469+297900 sites), 1840 bp average fragment length
   5% of sequence in fragments shorter than     613 bp
  10% of sequence in fragments shorter than     946 bp
  15% of sequence in fragments shorter than    1254 bp
  20% of sequence in fragments shorter than    1549 bp
  25% of sequence in fragments shorter than    1849 bp
  30% of sequence in fragments shorter than    2146 bp
  35% of sequence in fragments shorter than    2463 bp
  40% of sequence in fragments shorter than    2794 bp
  45% of sequence in fragments shorter than    3146 bp
  50% of sequence in fragments shorter than    3526 bp
  55% of sequence in fragments shorter than    3940 bp
  60% of sequence in fragments shorter than    4397 bp
  65% of sequence in fragments shorter than    4906 bp
  70% of sequence in fragments shorter than    5480 bp
  75% of sequence in fragments shorter than    6150 bp
  80% of sequence in fragments shorter than    6978 bp
  85% of sequence in fragments shorter than    8045 bp
  90% of sequence in fragments shorter than    9592 bp
  95% of sequence in fragments shorter than   12476 bp

# EsaBC3I.SgrAI (TCGA+CRCCGGYG) double digest, 1618 thousand fragments (1603346+15270 sites), 1841 bp average fragment length
   5% of sequence in fragments shorter than     628 bp
  10% of sequence in fragments shorter than     963 bp
  15% of sequence in fragments shorter than    1262 bp
  20% of sequence in fragments shorter than    1538 bp
  25% of sequence in fragments shorter than    1820 bp
  30% of sequence in fragments shorter than    2108 bp
  35% of sequence in fragments shorter than    2404 bp
  40% of sequence in fragments shorter than    2712 bp
  45% of sequence in fragments shorter than    3031 bp
  50% of sequence in fragments shorter than    3372 bp
  55% of sequence in fragments shorter than    3743 bp
  60% of sequence in fragments shorter than    4148 bp
  65% of sequence in fragments shorter than    4604 bp
  70% of sequence in fragments shorter than    5119 bp
  75% of sequence in fragments shorter than    5728 bp
  80% of sequence in fragments shorter than    6464 bp
  85% of sequence in fragments shorter than    7406 bp
  90% of sequence in fragments shorter than    8703 bp
  95% of sequence in fragments shorter than   10978 bp

# EsaBC3I.NruI (TCGA+TCGCGA) double digest, 1617 thousand fragments (1603346+14872 sites), 1842 bp average fragment length
   5% of sequence in fragments shorter than     628 bp
  10% of sequence in fragments shorter than     963 bp
  15% of sequence in fragments shorter than    1262 bp
  20% of sequence in fragments shorter than    1537 bp
  25% of sequence in fragments shorter than    1819 bp
  30% of sequence in fragments shorter than    2107 bp
  35% of sequence in fragments shorter than    2402 bp
  40% of sequence in fragments shorter than    2710 bp
  45% of sequence in fragments shorter than    3029 bp
  50% of sequence in fragments shorter than    3369 bp
  55% of sequence in fragments shorter than    3739 bp
  60% of sequence in fragments shorter than    4143 bp
  65% of sequence in fragments shorter than    4600 bp
  70% of sequence in fragments shorter than    5114 bp
  75% of sequence in fragments shorter than    5721 bp
  80% of sequence in fragments shorter than    6456 bp
  85% of sequence in fragments shorter than    7394 bp
  90% of sequence in fragments shorter than    8687 bp
  95% of sequence in fragments shorter than   10955 bp

# HgiCI.SacII (GGYRCC+CCGCGG) double digest, 1617 thousand fragments (1550877+66901 sites), 1842 bp average fragment length
   5% of sequence in fragments shorter than     608 bp
  10% of sequence in fragments shorter than     967 bp
  15% of sequence in fragments shorter than    1265 bp
  20% of sequence in fragments shorter than    1577 bp
  25% of sequence in fragments shorter than    1881 bp
  30% of sequence in fragments shorter than    2205 bp
  35% of sequence in fragments shorter than    2542 bp
  40% of sequence in fragments shorter than    2879 bp
  45% of sequence in fragments shorter than    3244 bp
  50% of sequence in fragments shorter than    3650 bp
  55% of sequence in fragments shorter than    4091 bp
  60% of sequence in fragments shorter than    4576 bp
  65% of sequence in fragments shorter than    5133 bp
  70% of sequence in fragments shorter than    5771 bp
  75% of sequence in fragments shorter than    6537 bp
  80% of sequence in fragments shorter than    7495 bp
  85% of sequence in fragments shorter than    8750 bp
  90% of sequence in fragments shorter than   10615 bp
  95% of sequence in fragments shorter than   14592 bp

# AflIII.SnaBI (ACRYGT+TACGTA) double digest, 1617 thousand fragments (1485394+132299 sites), 1842 bp average fragment length
   5% of sequence in fragments shorter than     668 bp
  10% of sequence in fragments shorter than    1037 bp
  15% of sequence in fragments shorter than    1354 bp
  20% of sequence in fragments shorter than    1650 bp
  25% of sequence in fragments shorter than    1934 bp
  30% of sequence in fragments shorter than    2217 bp
  35% of sequence in fragments shorter than    2511 bp
  40% of sequence in fragments shorter than    2807 bp
  45% of sequence in fragments shorter than    3116 bp
  50% of sequence in fragments shorter than    3453 bp
  55% of sequence in fragments shorter than    3809 bp
  60% of sequence in fragments shorter than    4203 bp
  65% of sequence in fragments shorter than    4643 bp
  70% of sequence in fragments shorter than    5143 bp
  75% of sequence in fragments shorter than    5729 bp
  80% of sequence in fragments shorter than    6415 bp
  85% of sequence in fragments shorter than    7288 bp
  90% of sequence in fragments shorter than    8543 bp
  95% of sequence in fragments shorter than   10870 bp

# EsaBC3I.PvuI (TCGA+CGATCG) double digest, 1617 thousand fragments (1603346+14186 sites), 1842 bp average fragment length
   5% of sequence in fragments shorter than     631 bp
  10% of sequence in fragments shorter than     967 bp
  15% of sequence in fragments shorter than    1266 bp
  20% of sequence in fragments shorter than    1543 bp
  25% of sequence in fragments shorter than    1825 bp
  30% of sequence in fragments shorter than    2114 bp
  35% of sequence in fragments shorter than    2410 bp
  40% of sequence in fragments shorter than    2718 bp
  45% of sequence in fragments shorter than    3037 bp
  50% of sequence in fragments shorter than    3378 bp
  55% of sequence in fragments shorter than    3749 bp
  60% of sequence in fragments shorter than    4154 bp
  65% of sequence in fragments shorter than    4610 bp
  70% of sequence in fragments shorter than    5124 bp
  75% of sequence in fragments shorter than    5734 bp
  80% of sequence in fragments shorter than    6469 bp
  85% of sequence in fragments shorter than    7409 bp
  90% of sequence in fragments shorter than    8703 bp
  95% of sequence in fragments shorter than   10960 bp

# BsaAI.NdeI (YACGTR+CATATG) double digest, 1616 thousand fragments (713801+903360 sites), 1843 bp average fragment length
   5% of sequence in fragments shorter than     648 bp
  10% of sequence in fragments shorter than     976 bp
  15% of sequence in fragments shorter than    1266 bp
  20% of sequence in fragments shorter than    1553 bp
  25% of sequence in fragments shorter than    1830 bp
  30% of sequence in fragments shorter than    2103 bp
  35% of sequence in fragments shorter than    2388 bp
  40% of sequence in fragments shorter than    2668 bp
  45% of sequence in fragments shorter than    2964 bp
  50% of sequence in fragments shorter than    3285 bp
  55% of sequence in fragments shorter than    3622 bp
  60% of sequence in fragments shorter than    3999 bp
  65% of sequence in fragments shorter than    4415 bp
  70% of sequence in fragments shorter than    4880 bp
  75% of sequence in fragments shorter than    5428 bp
  80% of sequence in fragments shorter than    6088 bp
  85% of sequence in fragments shorter than    6930 bp
  90% of sequence in fragments shorter than    8134 bp
  95% of sequence in fragments shorter than   10343 bp

# EsaBC3I.FseI (TCGA+GGCCGGCC) double digest, 1616 thousand fragments (1603346+13662 sites), 1843 bp average fragment length
   5% of sequence in fragments shorter than     629 bp
  10% of sequence in fragments shorter than     964 bp
  15% of sequence in fragments shorter than    1263 bp
  20% of sequence in fragments shorter than    1539 bp
  25% of sequence in fragments shorter than    1820 bp
  30% of sequence in fragments shorter than    2109 bp
  35% of sequence in fragments shorter than    2405 bp
  40% of sequence in fragments shorter than    2712 bp
  45% of sequence in fragments shorter than    3031 bp
  50% of sequence in fragments shorter than    3373 bp
  55% of sequence in fragments shorter than    3743 bp
  60% of sequence in fragments shorter than    4148 bp
  65% of sequence in fragments shorter than    4605 bp
  70% of sequence in fragments shorter than    5120 bp
  75% of sequence in fragments shorter than    5729 bp
  80% of sequence in fragments shorter than    6467 bp
  85% of sequence in fragments shorter than    7410 bp
  90% of sequence in fragments shorter than    8707 bp
  95% of sequence in fragments shorter than   10988 bp

# BsmI.SacII (GAATGC+CCGCGG) double digest, 1615 thousand fragments (1549350+66901 sites), 1844 bp average fragment length
   5% of sequence in fragments shorter than     647 bp
  10% of sequence in fragments shorter than    1108 bp
  15% of sequence in fragments shorter than    1497 bp
  20% of sequence in fragments shorter than    1852 bp
  25% of sequence in fragments shorter than    2192 bp
  30% of sequence in fragments shorter than    2519 bp
  35% of sequence in fragments shorter than    2850 bp
  40% of sequence in fragments shorter than    3192 bp
  45% of sequence in fragments shorter than    3554 bp
  50% of sequence in fragments shorter than    3934 bp
  55% of sequence in fragments shorter than    4340 bp
  60% of sequence in fragments shorter than    4778 bp
  65% of sequence in fragments shorter than    5264 bp
  70% of sequence in fragments shorter than    5812 bp
  75% of sequence in fragments shorter than    6435 bp
  80% of sequence in fragments shorter than    7177 bp
  85% of sequence in fragments shorter than    8114 bp
  90% of sequence in fragments shorter than    9411 bp
  95% of sequence in fragments shorter than   11567 bp

# EsaBC3I.NotI (TCGA+GCGGCCGC) double digest, 1612 thousand fragments (1603346+9795 sites), 1847 bp average fragment length
   5% of sequence in fragments shorter than     630 bp
  10% of sequence in fragments shorter than     966 bp
  15% of sequence in fragments shorter than    1266 bp
  20% of sequence in fragments shorter than    1543 bp
  25% of sequence in fragments shorter than    1826 bp
  30% of sequence in fragments shorter than    2115 bp
  35% of sequence in fragments shorter than    2413 bp
  40% of sequence in fragments shorter than    2722 bp
  45% of sequence in fragments shorter than    3042 bp
  50% of sequence in fragments shorter than    3383 bp
  55% of sequence in fragments shorter than    3755 bp
  60% of sequence in fragments shorter than    4161 bp
  65% of sequence in fragments shorter than    4619 bp
  70% of sequence in fragments shorter than    5136 bp
  75% of sequence in fragments shorter than    5746 bp
  80% of sequence in fragments shorter than    6484 bp
  85% of sequence in fragments shorter than    7428 bp
  90% of sequence in fragments shorter than    8726 bp
  95% of sequence in fragments shorter than   11006 bp

# EsaBC3I.RsrII (TCGA+CGGWCCG) double digest, 1612 thousand fragments (1603346+9764 sites), 1847 bp average fragment length
   5% of sequence in fragments shorter than     630 bp
  10% of sequence in fragments shorter than     966 bp
  15% of sequence in fragments shorter than    1265 bp
  20% of sequence in fragments shorter than    1542 bp
  25% of sequence in fragments shorter than    1825 bp
  30% of sequence in fragments shorter than    2113 bp
  35% of sequence in fragments shorter than    2410 bp
  40% of sequence in fragments shorter than    2719 bp
  45% of sequence in fragments shorter than    3038 bp
  50% of sequence in fragments shorter than    3379 bp
  55% of sequence in fragments shorter than    3750 bp
  60% of sequence in fragments shorter than    4156 bp
  65% of sequence in fragments shorter than    4613 bp
  70% of sequence in fragments shorter than    5129 bp
  75% of sequence in fragments shorter than    5738 bp
  80% of sequence in fragments shorter than    6475 bp
  85% of sequence in fragments shorter than    7418 bp
  90% of sequence in fragments shorter than    8716 bp
  95% of sequence in fragments shorter than   10995 bp

# AclI.FauI (AACGTT+CCCGC) double digest, 1612 thousand fragments (172706+1439772 sites), 1848 bp average fragment length
   5% of sequence in fragments shorter than     643 bp
  10% of sequence in fragments shorter than    1101 bp
  15% of sequence in fragments shorter than    1535 bp
  20% of sequence in fragments shorter than    1973 bp
  25% of sequence in fragments shorter than    2413 bp
  30% of sequence in fragments shorter than    2881 bp
  35% of sequence in fragments shorter than    3353 bp
  40% of sequence in fragments shorter than    3875 bp
  45% of sequence in fragments shorter than    4420 bp
  50% of sequence in fragments shorter than    5031 bp
  55% of sequence in fragments shorter than    5705 bp
  60% of sequence in fragments shorter than    6460 bp
  65% of sequence in fragments shorter than    7308 bp
  70% of sequence in fragments shorter than    8272 bp
  75% of sequence in fragments shorter than    9418 bp
  80% of sequence in fragments shorter than   10816 bp
  85% of sequence in fragments shorter than   12617 bp
  90% of sequence in fragments shorter than   15245 bp
  95% of sequence in fragments shorter than   20037 bp

# AflIII.NaeI (ACRYGT+GCCGGC) double digest, 1610 thousand fragments (1485394+125692 sites), 1850 bp average fragment length
   5% of sequence in fragments shorter than     665 bp
  10% of sequence in fragments shorter than    1037 bp
  15% of sequence in fragments shorter than    1357 bp
  20% of sequence in fragments shorter than    1659 bp
  25% of sequence in fragments shorter than    1946 bp
  30% of sequence in fragments shorter than    2231 bp
  35% of sequence in fragments shorter than    2523 bp
  40% of sequence in fragments shorter than    2820 bp
  45% of sequence in fragments shorter than    3130 bp
  50% of sequence in fragments shorter than    3467 bp
  55% of sequence in fragments shorter than    3828 bp
  60% of sequence in fragments shorter than    4223 bp
  65% of sequence in fragments shorter than    4659 bp
  70% of sequence in fragments shorter than    5156 bp
  75% of sequence in fragments shorter than    5732 bp
  80% of sequence in fragments shorter than    6409 bp
  85% of sequence in fragments shorter than    7267 bp
  90% of sequence in fragments shorter than    8497 bp
  95% of sequence in fragments shorter than   10773 bp

# AclI.BspMI (AACGTT+ACCTGC) double digest, 1610 thousand fragments (172706+1438129 sites), 1850 bp average fragment length
   5% of sequence in fragments shorter than     635 bp
  10% of sequence in fragments shorter than     966 bp
  15% of sequence in fragments shorter than    1262 bp
  20% of sequence in fragments shorter than    1546 bp
  25% of sequence in fragments shorter than    1837 bp
  30% of sequence in fragments shorter than    2107 bp
  35% of sequence in fragments shorter than    2395 bp
  40% of sequence in fragments shorter than    2704 bp
  45% of sequence in fragments shorter than    3024 bp
  50% of sequence in fragments shorter than    3337 bp
  55% of sequence in fragments shorter than    3704 bp
  60% of sequence in fragments shorter than    4111 bp
  65% of sequence in fragments shorter than    4560 bp
  70% of sequence in fragments shorter than    5068 bp
  75% of sequence in fragments shorter than    5661 bp
  80% of sequence in fragments shorter than    6366 bp
  85% of sequence in fragments shorter than    7278 bp
  90% of sequence in fragments shorter than    8556 bp
  95% of sequence in fragments shorter than   10705 bp

# NcoI.XbaI (CCATGG+TCTAGA) double digest, 1610 thousand fragments (759594+850998 sites), 1850 bp average fragment length
   5% of sequence in fragments shorter than     649 bp
  10% of sequence in fragments shorter than     973 bp
  15% of sequence in fragments shorter than    1278 bp
  20% of sequence in fragments shorter than    1543 bp
  25% of sequence in fragments shorter than    1813 bp
  30% of sequence in fragments shorter than    2081 bp
  35% of sequence in fragments shorter than    2342 bp
  40% of sequence in fragments shorter than    2625 bp
  45% of sequence in fragments shorter than    2924 bp
  50% of sequence in fragments shorter than    3231 bp
  55% of sequence in fragments shorter than    3565 bp
  60% of sequence in fragments shorter than    3932 bp
  65% of sequence in fragments shorter than    4336 bp
  70% of sequence in fragments shorter than    4795 bp
  75% of sequence in fragments shorter than    5325 bp
  80% of sequence in fragments shorter than    5968 bp
  85% of sequence in fragments shorter than    6786 bp
  90% of sequence in fragments shorter than    7950 bp
  95% of sequence in fragments shorter than   10020 bp

# HgiCI.PspXI (GGYRCC+VCTCGAGB) double digest, 1609 thousand fragments (1550877+59193 sites), 1851 bp average fragment length
   5% of sequence in fragments shorter than     606 bp
  10% of sequence in fragments shorter than     956 bp
  15% of sequence in fragments shorter than    1247 bp
  20% of sequence in fragments shorter than    1551 bp
  25% of sequence in fragments shorter than    1846 bp
  30% of sequence in fragments shorter than    2163 bp
  35% of sequence in fragments shorter than    2490 bp
  40% of sequence in fragments shorter than    2824 bp
  45% of sequence in fragments shorter than    3175 bp
  50% of sequence in fragments shorter than    3570 bp
  55% of sequence in fragments shorter than    4002 bp
  60% of sequence in fragments shorter than    4475 bp
  65% of sequence in fragments shorter than    5017 bp
  70% of sequence in fragments shorter than    5646 bp
  75% of sequence in fragments shorter than    6392 bp
  80% of sequence in fragments shorter than    7331 bp
  85% of sequence in fragments shorter than    8548 bp
  90% of sequence in fragments shorter than   10362 bp
  95% of sequence in fragments shorter than   14177 bp

# KpnI.PstI (GGTACC+CTGCAG) double digest, 1609 thousand fragments (287974+1321469 sites), 1852 bp average fragment length
   5% of sequence in fragments shorter than     626 bp
  10% of sequence in fragments shorter than     967 bp
  15% of sequence in fragments shorter than    1258 bp
  20% of sequence in fragments shorter than    1557 bp
  25% of sequence in fragments shorter than    1842 bp
  30% of sequence in fragments shorter than    2129 bp
  35% of sequence in fragments shorter than    2440 bp
  40% of sequence in fragments shorter than    2755 bp
  45% of sequence in fragments shorter than    3087 bp
  50% of sequence in fragments shorter than    3449 bp
  55% of sequence in fragments shorter than    3843 bp
  60% of sequence in fragments shorter than    4278 bp
  65% of sequence in fragments shorter than    4772 bp
  70% of sequence in fragments shorter than    5330 bp
  75% of sequence in fragments shorter than    5988 bp
  80% of sequence in fragments shorter than    6795 bp
  85% of sequence in fragments shorter than    7832 bp
  90% of sequence in fragments shorter than    9345 bp
  95% of sequence in fragments shorter than   12142 bp

# AvaI.SacI (CYCGRG+GAGCTC) double digest, 1608 thousand fragments (1005714+603323 sites), 1852 bp average fragment length
   5% of sequence in fragments shorter than     605 bp
  10% of sequence in fragments shorter than     974 bp
  15% of sequence in fragments shorter than    1313 bp
  20% of sequence in fragments shorter than    1647 bp
  25% of sequence in fragments shorter than    1988 bp
  30% of sequence in fragments shorter than    2338 bp
  35% of sequence in fragments shorter than    2714 bp
  40% of sequence in fragments shorter than    3118 bp
  45% of sequence in fragments shorter than    3551 bp
  50% of sequence in fragments shorter than    4026 bp
  55% of sequence in fragments shorter than    4536 bp
  60% of sequence in fragments shorter than    5104 bp
  65% of sequence in fragments shorter than    5752 bp
  70% of sequence in fragments shorter than    6500 bp
  75% of sequence in fragments shorter than    7397 bp
  80% of sequence in fragments shorter than    8510 bp
  85% of sequence in fragments shorter than    9985 bp
  90% of sequence in fragments shorter than   12155 bp
  95% of sequence in fragments shorter than   16429 bp

# NheI.PstI (GCTAGC+CTGCAG) double digest, 1608 thousand fragments (287384+1321469 sites), 1852 bp average fragment length
   5% of sequence in fragments shorter than     634 bp
  10% of sequence in fragments shorter than     983 bp
  15% of sequence in fragments shorter than    1293 bp
  20% of sequence in fragments shorter than    1574 bp
  25% of sequence in fragments shorter than    1844 bp
  30% of sequence in fragments shorter than    2129 bp
  35% of sequence in fragments shorter than    2429 bp
  40% of sequence in fragments shorter than    2749 bp
  45% of sequence in fragments shorter than    3079 bp
  50% of sequence in fragments shorter than    3438 bp
  55% of sequence in fragments shorter than    3825 bp
  60% of sequence in fragments shorter than    4254 bp
  65% of sequence in fragments shorter than    4736 bp
  70% of sequence in fragments shorter than    5282 bp
  75% of sequence in fragments shorter than    5929 bp
  80% of sequence in fragments shorter than    6717 bp
  85% of sequence in fragments shorter than    7740 bp
  90% of sequence in fragments shorter than    9215 bp
  95% of sequence in fragments shorter than   11913 bp

# BceAI.NdeI (ACGGC+CATATG) double digest, 1608 thousand fragments (705178+903360 sites), 1853 bp average fragment length
   5% of sequence in fragments shorter than     642 bp
  10% of sequence in fragments shorter than     984 bp
  15% of sequence in fragments shorter than    1271 bp
  20% of sequence in fragments shorter than    1560 bp
  25% of sequence in fragments shorter than    1844 bp
  30% of sequence in fragments shorter than    2126 bp
  35% of sequence in fragments shorter than    2416 bp
  40% of sequence in fragments shorter than    2713 bp
  45% of sequence in fragments shorter than    3023 bp
  50% of sequence in fragments shorter than    3350 bp
  55% of sequence in fragments shorter than    3701 bp
  60% of sequence in fragments shorter than    4066 bp
  65% of sequence in fragments shorter than    4475 bp
  70% of sequence in fragments shorter than    4948 bp
  75% of sequence in fragments shorter than    5498 bp
  80% of sequence in fragments shorter than    6163 bp
  85% of sequence in fragments shorter than    7020 bp
  90% of sequence in fragments shorter than    8255 bp
  95% of sequence in fragments shorter than   10526 bp

# BsmI.PspXI (GAATGC+VCTCGAGB) double digest, 1608 thousand fragments (1549350+59193 sites), 1853 bp average fragment length
   5% of sequence in fragments shorter than     656 bp
  10% of sequence in fragments shorter than    1111 bp
  15% of sequence in fragments shorter than    1494 bp
  20% of sequence in fragments shorter than    1844 bp
  25% of sequence in fragments shorter than    2179 bp
  30% of sequence in fragments shorter than    2502 bp
  35% of sequence in fragments shorter than    2827 bp
  40% of sequence in fragments shorter than    3165 bp
  45% of sequence in fragments shorter than    3521 bp
  50% of sequence in fragments shorter than    3897 bp
  55% of sequence in fragments shorter than    4298 bp
  60% of sequence in fragments shorter than    4732 bp
  65% of sequence in fragments shorter than    5213 bp
  70% of sequence in fragments shorter than    5757 bp
  75% of sequence in fragments shorter than    6375 bp
  80% of sequence in fragments shorter than    7112 bp
  85% of sequence in fragments shorter than    8048 bp
  90% of sequence in fragments shorter than    9332 bp
  95% of sequence in fragments shorter than   11473 bp

# BspHI.HaeII (TCATGA+RGCGCY) double digest, 1607 thousand fragments (978290+630101 sites), 1853 bp average fragment length
   5% of sequence in fragments shorter than     642 bp
  10% of sequence in fragments shorter than     982 bp
  15% of sequence in fragments shorter than    1306 bp
  20% of sequence in fragments shorter than    1607 bp
  25% of sequence in fragments shorter than    1896 bp
  30% of sequence in fragments shorter than    2178 bp
  35% of sequence in fragments shorter than    2465 bp
  40% of sequence in fragments shorter than    2769 bp
  45% of sequence in fragments shorter than    3084 bp
  50% of sequence in fragments shorter than    3408 bp
  55% of sequence in fragments shorter than    3763 bp
  60% of sequence in fragments shorter than    4139 bp
  65% of sequence in fragments shorter than    4562 bp
  70% of sequence in fragments shorter than    5037 bp
  75% of sequence in fragments shorter than    5586 bp
  80% of sequence in fragments shorter than    6243 bp
  85% of sequence in fragments shorter than    7062 bp
  90% of sequence in fragments shorter than    8218 bp
  95% of sequence in fragments shorter than   10191 bp

# BscGI.SapI (CCCGT+GCTCTTC) double digest, 1607 thousand fragments (1231070+377161 sites), 1853 bp average fragment length
   5% of sequence in fragments shorter than     543 bp
  10% of sequence in fragments shorter than     916 bp
  15% of sequence in fragments shorter than    1273 bp
  20% of sequence in fragments shorter than    1617 bp
  25% of sequence in fragments shorter than    1963 bp
  30% of sequence in fragments shorter than    2316 bp
  35% of sequence in fragments shorter than    2681 bp
  40% of sequence in fragments shorter than    3066 bp
  45% of sequence in fragments shorter than    3474 bp
  50% of sequence in fragments shorter than    3909 bp
  55% of sequence in fragments shorter than    4377 bp
  60% of sequence in fragments shorter than    4898 bp
  65% of sequence in fragments shorter than    5486 bp
  70% of sequence in fragments shorter than    6154 bp
  75% of sequence in fragments shorter than    6935 bp
  80% of sequence in fragments shorter than    7885 bp
  85% of sequence in fragments shorter than    9130 bp
  90% of sequence in fragments shorter than   10869 bp
  95% of sequence in fragments shorter than   13908 bp

# AscI.EsaBC3I (GGCGCGCC+TCGA) double digest, 1607 thousand fragments (4751+1603346 sites), 1853 bp average fragment length
   5% of sequence in fragments shorter than     633 bp
  10% of sequence in fragments shorter than     969 bp
  15% of sequence in fragments shorter than    1269 bp
  20% of sequence in fragments shorter than    1547 bp
  25% of sequence in fragments shorter than    1831 bp
  30% of sequence in fragments shorter than    2119 bp
  35% of sequence in fragments shorter than    2417 bp
  40% of sequence in fragments shorter than    2726 bp
  45% of sequence in fragments shorter than    3047 bp
  50% of sequence in fragments shorter than    3388 bp
  55% of sequence in fragments shorter than    3760 bp
  60% of sequence in fragments shorter than    4167 bp
  65% of sequence in fragments shorter than    4625 bp
  70% of sequence in fragments shorter than    5142 bp
  75% of sequence in fragments shorter than    5751 bp
  80% of sequence in fragments shorter than    6489 bp
  85% of sequence in fragments shorter than    7433 bp
  90% of sequence in fragments shorter than    8734 bp
  95% of sequence in fragments shorter than   11011 bp

# BscGI.SmaI (CCCGT+CCCGGG) double digest, 1607 thousand fragments (1231070+376940 sites), 1853 bp average fragment length
   5% of sequence in fragments shorter than     519 bp
  10% of sequence in fragments shorter than     925 bp
  15% of sequence in fragments shorter than    1327 bp
  20% of sequence in fragments shorter than    1729 bp
  25% of sequence in fragments shorter than    2140 bp
  30% of sequence in fragments shorter than    2574 bp
  35% of sequence in fragments shorter than    3031 bp
  40% of sequence in fragments shorter than    3522 bp
  45% of sequence in fragments shorter than    4047 bp
  50% of sequence in fragments shorter than    4616 bp
  55% of sequence in fragments shorter than    5250 bp
  60% of sequence in fragments shorter than    5957 bp
  65% of sequence in fragments shorter than    6744 bp
  70% of sequence in fragments shorter than    7664 bp
  75% of sequence in fragments shorter than    8747 bp
  80% of sequence in fragments shorter than   10077 bp
  85% of sequence in fragments shorter than   11816 bp
  90% of sequence in fragments shorter than   14264 bp
  95% of sequence in fragments shorter than   18500 bp

# EcoRI.NcoI (GAATTC+CCATGG) double digest, 1606 thousand fragments (847401+759594 sites), 1854 bp average fragment length
   5% of sequence in fragments shorter than     644 bp
  10% of sequence in fragments shorter than     975 bp
  15% of sequence in fragments shorter than    1278 bp
  20% of sequence in fragments shorter than    1549 bp
  25% of sequence in fragments shorter than    1826 bp
  30% of sequence in fragments shorter than    2098 bp
  35% of sequence in fragments shorter than    2349 bp
  40% of sequence in fragments shorter than    2630 bp
  45% of sequence in fragments shorter than    2922 bp
  50% of sequence in fragments shorter than    3233 bp
  55% of sequence in fragments shorter than    3567 bp
  60% of sequence in fragments shorter than    3932 bp
  65% of sequence in fragments shorter than    4333 bp
  70% of sequence in fragments shorter than    4784 bp
  75% of sequence in fragments shorter than    5310 bp
  80% of sequence in fragments shorter than    5932 bp
  85% of sequence in fragments shorter than    6730 bp
  90% of sequence in fragments shorter than    7867 bp
  95% of sequence in fragments shorter than    9847 bp

# AflIII.XhoI (ACRYGT+CTCGAG) double digest, 1606 thousand fragments (1485394+121308 sites), 1855 bp average fragment length
   5% of sequence in fragments shorter than     670 bp
  10% of sequence in fragments shorter than    1041 bp
  15% of sequence in fragments shorter than    1356 bp
  20% of sequence in fragments shorter than    1654 bp
  25% of sequence in fragments shorter than    1936 bp
  30% of sequence in fragments shorter than    2215 bp
  35% of sequence in fragments shorter than    2503 bp
  40% of sequence in fragments shorter than    2795 bp
  45% of sequence in fragments shorter than    3100 bp
  50% of sequence in fragments shorter than    3423 bp
  55% of sequence in fragments shorter than    3773 bp
  60% of sequence in fragments shorter than    4129 bp
  65% of sequence in fragments shorter than    4536 bp
  70% of sequence in fragments shorter than    5007 bp
  75% of sequence in fragments shorter than    5560 bp
  80% of sequence in fragments shorter than    6226 bp
  85% of sequence in fragments shorter than    7075 bp
  90% of sequence in fragments shorter than    8304 bp
  95% of sequence in fragments shorter than   10545 bp

# AgeI.HgiCI (ACCGGT+GGYRCC) double digest, 1603 thousand fragments (53386+1550877 sites), 1857 bp average fragment length
   5% of sequence in fragments shorter than     613 bp
  10% of sequence in fragments shorter than     963 bp
  15% of sequence in fragments shorter than    1256 bp
  20% of sequence in fragments shorter than    1561 bp
  25% of sequence in fragments shorter than    1856 bp
  30% of sequence in fragments shorter than    2172 bp
  35% of sequence in fragments shorter than    2502 bp
  40% of sequence in fragments shorter than    2836 bp
  45% of sequence in fragments shorter than    3188 bp
  50% of sequence in fragments shorter than    3583 bp
  55% of sequence in fragments shorter than    4016 bp
  60% of sequence in fragments shorter than    4486 bp
  65% of sequence in fragments shorter than    5029 bp
  70% of sequence in fragments shorter than    5651 bp
  75% of sequence in fragments shorter than    6396 bp
  80% of sequence in fragments shorter than    7333 bp
  85% of sequence in fragments shorter than    8552 bp
  90% of sequence in fragments shorter than   10372 bp
  95% of sequence in fragments shorter than   14195 bp

# AgeI.BsmI (ACCGGT+GAATGC) double digest, 1602 thousand fragments (53386+1549350 sites), 1859 bp average fragment length
   5% of sequence in fragments shorter than     660 bp
  10% of sequence in fragments shorter than    1110 bp
  15% of sequence in fragments shorter than    1497 bp
  20% of sequence in fragments shorter than    1854 bp
  25% of sequence in fragments shorter than    2183 bp
  30% of sequence in fragments shorter than    2510 bp
  35% of sequence in fragments shorter than    2843 bp
  40% of sequence in fragments shorter than    3185 bp
  45% of sequence in fragments shorter than    3546 bp
  50% of sequence in fragments shorter than    3924 bp
  55% of sequence in fragments shorter than    4328 bp
  60% of sequence in fragments shorter than    4767 bp
  65% of sequence in fragments shorter than    5250 bp
  70% of sequence in fragments shorter than    5797 bp
  75% of sequence in fragments shorter than    6421 bp
  80% of sequence in fragments shorter than    7166 bp
  85% of sequence in fragments shorter than    8113 bp
  90% of sequence in fragments shorter than    9409 bp
  95% of sequence in fragments shorter than   11588 bp

# AvaI.AvrII (CYCGRG+CCTAGG) double digest, 1600 thousand fragments (1005714+594956 sites), 1862 bp average fragment length
   5% of sequence in fragments shorter than     621 bp
  10% of sequence in fragments shorter than     971 bp
  15% of sequence in fragments shorter than    1308 bp
  20% of sequence in fragments shorter than    1635 bp
  25% of sequence in fragments shorter than    1966 bp
  30% of sequence in fragments shorter than    2306 bp
  35% of sequence in fragments shorter than    2659 bp
  40% of sequence in fragments shorter than    3006 bp
  45% of sequence in fragments shorter than    3401 bp
  50% of sequence in fragments shorter than    3820 bp
  55% of sequence in fragments shorter than    4285 bp
  60% of sequence in fragments shorter than    4802 bp
  65% of sequence in fragments shorter than    5390 bp
  70% of sequence in fragments shorter than    6077 bp
  75% of sequence in fragments shorter than    6887 bp
  80% of sequence in fragments shorter than    7902 bp
  85% of sequence in fragments shorter than    9239 bp
  90% of sequence in fragments shorter than   11256 bp
  95% of sequence in fragments shorter than   15417 bp

# BciVI.NdeI (GTATCC+CATATG) double digest, 1599 thousand fragments (696093+903360 sites), 1863 bp average fragment length
   5% of sequence in fragments shorter than     632 bp
  10% of sequence in fragments shorter than     975 bp
  15% of sequence in fragments shorter than    1263 bp
  20% of sequence in fragments shorter than    1553 bp
  25% of sequence in fragments shorter than    1836 bp
  30% of sequence in fragments shorter than    2117 bp
  35% of sequence in fragments shorter than    2415 bp
  40% of sequence in fragments shorter than    2721 bp
  45% of sequence in fragments shorter than    3041 bp
  50% of sequence in fragments shorter than    3380 bp
  55% of sequence in fragments shorter than    3748 bp
  60% of sequence in fragments shorter than    4150 bp
  65% of sequence in fragments shorter than    4591 bp
  70% of sequence in fragments shorter than    5100 bp
  75% of sequence in fragments shorter than    5701 bp
  80% of sequence in fragments shorter than    6439 bp
  85% of sequence in fragments shorter than    7431 bp
  90% of sequence in fragments shorter than    8917 bp
  95% of sequence in fragments shorter than   11883 bp

# FauI.PacI (CCCGC+TTAATTAA) double digest, 1598 thousand fragments (1439772+159455 sites), 1863 bp average fragment length
   5% of sequence in fragments shorter than     658 bp
  10% of sequence in fragments shorter than    1137 bp
  15% of sequence in fragments shorter than    1575 bp
  20% of sequence in fragments shorter than    2018 bp
  25% of sequence in fragments shorter than    2463 bp
  30% of sequence in fragments shorter than    2929 bp
  35% of sequence in fragments shorter than    3419 bp
  40% of sequence in fragments shorter than    3943 bp
  45% of sequence in fragments shorter than    4472 bp
  50% of sequence in fragments shorter than    5075 bp
  55% of sequence in fragments shorter than    5738 bp
  60% of sequence in fragments shorter than    6471 bp
  65% of sequence in fragments shorter than    7292 bp
  70% of sequence in fragments shorter than    8236 bp
  75% of sequence in fragments shorter than    9361 bp
  80% of sequence in fragments shorter than   10737 bp
  85% of sequence in fragments shorter than   12519 bp
  90% of sequence in fragments shorter than   15122 bp
  95% of sequence in fragments shorter than   20139 bp

# BclI.HindIII (TGATCA+AAGCTT) double digest, 1598 thousand fragments (738785+860365 sites), 1864 bp average fragment length
   5% of sequence in fragments shorter than     656 bp
  10% of sequence in fragments shorter than     991 bp
  15% of sequence in fragments shorter than    1282 bp
  20% of sequence in fragments shorter than    1553 bp
  25% of sequence in fragments shorter than    1815 bp
  30% of sequence in fragments shorter than    2056 bp
  35% of sequence in fragments shorter than    2329 bp
  40% of sequence in fragments shorter than    2606 bp
  45% of sequence in fragments shorter than    2894 bp
  50% of sequence in fragments shorter than    3200 bp
  55% of sequence in fragments shorter than    3532 bp
  60% of sequence in fragments shorter than    3893 bp
  65% of sequence in fragments shorter than    4287 bp
  70% of sequence in fragments shorter than    4744 bp
  75% of sequence in fragments shorter than    5281 bp
  80% of sequence in fragments shorter than    5937 bp
  85% of sequence in fragments shorter than    6786 bp
  90% of sequence in fragments shorter than    8027 bp
  95% of sequence in fragments shorter than   10439 bp

# FauI.Hpy99I (CCCGC+CGWCG) double digest, 1597 thousand fragments (1439772+157917 sites), 1865 bp average fragment length
   5% of sequence in fragments shorter than     647 bp
  10% of sequence in fragments shorter than    1159 bp
  15% of sequence in fragments shorter than    1642 bp
  20% of sequence in fragments shorter than    2130 bp
  25% of sequence in fragments shorter than    2651 bp
  30% of sequence in fragments shorter than    3197 bp
  35% of sequence in fragments shorter than    3784 bp
  40% of sequence in fragments shorter than    4389 bp
  45% of sequence in fragments shorter than    5073 bp
  50% of sequence in fragments shorter than    5824 bp
  55% of sequence in fragments shorter than    6666 bp
  60% of sequence in fragments shorter than    7590 bp
  65% of sequence in fragments shorter than    8652 bp
  70% of sequence in fragments shorter than    9892 bp
  75% of sequence in fragments shorter than   11354 bp
  80% of sequence in fragments shorter than   13145 bp
  85% of sequence in fragments shorter than   15531 bp
  90% of sequence in fragments shorter than   19057 bp
  95% of sequence in fragments shorter than   25470 bp

# BspMI.PacI (ACCTGC+TTAATTAA) double digest, 1597 thousand fragments (1438129+159455 sites), 1865 bp average fragment length
   5% of sequence in fragments shorter than     643 bp
  10% of sequence in fragments shorter than     981 bp
  15% of sequence in fragments shorter than    1295 bp
  20% of sequence in fragments shorter than    1582 bp
  25% of sequence in fragments shorter than    1876 bp
  30% of sequence in fragments shorter than    2144 bp
  35% of sequence in fragments shorter than    2438 bp
  40% of sequence in fragments shorter than    2747 bp
  45% of sequence in fragments shorter than    3066 bp
  50% of sequence in fragments shorter than    3364 bp
  55% of sequence in fragments shorter than    3729 bp
  60% of sequence in fragments shorter than    4133 bp
  65% of sequence in fragments shorter than    4581 bp
  70% of sequence in fragments shorter than    5086 bp
  75% of sequence in fragments shorter than    5667 bp
  80% of sequence in fragments shorter than    6371 bp
  85% of sequence in fragments shorter than    7281 bp
  90% of sequence in fragments shorter than    8565 bp
  95% of sequence in fragments shorter than   10780 bp

# AccI.ApaLI (GTMKAC+GTGCAC) double digest, 1596 thousand fragments (1101063+496255 sites), 1866 bp average fragment length
   5% of sequence in fragments shorter than     674 bp
  10% of sequence in fragments shorter than    1022 bp
  15% of sequence in fragments shorter than    1317 bp
  20% of sequence in fragments shorter than    1590 bp
  25% of sequence in fragments shorter than    1861 bp
  30% of sequence in fragments shorter than    2127 bp
  35% of sequence in fragments shorter than    2401 bp
  40% of sequence in fragments shorter than    2680 bp
  45% of sequence in fragments shorter than    2967 bp
  50% of sequence in fragments shorter than    3278 bp
  55% of sequence in fragments shorter than    3607 bp
  60% of sequence in fragments shorter than    3972 bp
  65% of sequence in fragments shorter than    4368 bp
  70% of sequence in fragments shorter than    4815 bp
  75% of sequence in fragments shorter than    5336 bp
  80% of sequence in fragments shorter than    5923 bp
  85% of sequence in fragments shorter than    6658 bp
  90% of sequence in fragments shorter than    7764 bp
  95% of sequence in fragments shorter than    9761 bp

# BamHI.BscGI (GGATCC+CCCGT) double digest, 1596 thousand fragments (365999+1231070 sites), 1866 bp average fragment length
   5% of sequence in fragments shorter than     546 bp
  10% of sequence in fragments shorter than     932 bp
  15% of sequence in fragments shorter than    1294 bp
  20% of sequence in fragments shorter than    1644 bp
  25% of sequence in fragments shorter than    1998 bp
  30% of sequence in fragments shorter than    2362 bp
  35% of sequence in fragments shorter than    2738 bp
  40% of sequence in fragments shorter than    3131 bp
  45% of sequence in fragments shorter than    3537 bp
  50% of sequence in fragments shorter than    3975 bp
  55% of sequence in fragments shorter than    4457 bp
  60% of sequence in fragments shorter than    4983 bp
  65% of sequence in fragments shorter than    5594 bp
  70% of sequence in fragments shorter than    6294 bp
  75% of sequence in fragments shorter than    7116 bp
  80% of sequence in fragments shorter than    8111 bp
  85% of sequence in fragments shorter than    9407 bp
  90% of sequence in fragments shorter than   11212 bp
  95% of sequence in fragments shorter than   14306 bp

# BspMI.Hpy99I (ACCTGC+CGWCG) double digest, 1595 thousand fragments (1438129+157917 sites), 1867 bp average fragment length
   5% of sequence in fragments shorter than     625 bp
  10% of sequence in fragments shorter than     972 bp
  15% of sequence in fragments shorter than    1301 bp
  20% of sequence in fragments shorter than    1608 bp
  25% of sequence in fragments shorter than    1923 bp
  30% of sequence in fragments shorter than    2213 bp
  35% of sequence in fragments shorter than    2537 bp
  40% of sequence in fragments shorter than    2867 bp
  45% of sequence in fragments shorter than    3223 bp
  50% of sequence in fragments shorter than    3553 bp
  55% of sequence in fragments shorter than    3965 bp
  60% of sequence in fragments shorter than    4417 bp
  65% of sequence in fragments shorter than    4916 bp
  70% of sequence in fragments shorter than    5479 bp
  75% of sequence in fragments shorter than    6144 bp
  80% of sequence in fragments shorter than    6953 bp
  85% of sequence in fragments shorter than    7982 bp
  90% of sequence in fragments shorter than    9446 bp
  95% of sequence in fragments shorter than   11995 bp

# FnuDII.HindIII (CGCG+AAGCTT) double digest, 1593 thousand fragments (733940+860365 sites), 1869 bp average fragment length
   5% of sequence in fragments shorter than     705 bp
  10% of sequence in fragments shorter than    1140 bp
  15% of sequence in fragments shorter than    1513 bp
  20% of sequence in fragments shorter than    1860 bp
  25% of sequence in fragments shorter than    2170 bp
  30% of sequence in fragments shorter than    2508 bp
  35% of sequence in fragments shorter than    2848 bp
  40% of sequence in fragments shorter than    3199 bp
  45% of sequence in fragments shorter than    3568 bp
  50% of sequence in fragments shorter than    3959 bp
  55% of sequence in fragments shorter than    4378 bp
  60% of sequence in fragments shorter than    4829 bp
  65% of sequence in fragments shorter than    5331 bp
  70% of sequence in fragments shorter than    5901 bp
  75% of sequence in fragments shorter than    6567 bp
  80% of sequence in fragments shorter than    7375 bp
  85% of sequence in fragments shorter than    8410 bp
  90% of sequence in fragments shorter than    9873 bp
  95% of sequence in fragments shorter than   12518 bp

# HgiCI.PmeI (GGYRCC+GTTTAAAC) double digest, 1592 thousand fragments (1550877+42094 sites), 1871 bp average fragment length
   5% of sequence in fragments shorter than     617 bp
  10% of sequence in fragments shorter than     968 bp
  15% of sequence in fragments shorter than    1259 bp
  20% of sequence in fragments shorter than    1563 bp
  25% of sequence in fragments shorter than    1855 bp
  30% of sequence in fragments shorter than    2168 bp
  35% of sequence in fragments shorter than    2492 bp
  40% of sequence in fragments shorter than    2822 bp
  45% of sequence in fragments shorter than    3167 bp
  50% of sequence in fragments shorter than    3554 bp
  55% of sequence in fragments shorter than    3979 bp
  60% of sequence in fragments shorter than    4441 bp
  65% of sequence in fragments shorter than    4971 bp
  70% of sequence in fragments shorter than    5581 bp
  75% of sequence in fragments shorter than    6303 bp
  80% of sequence in fragments shorter than    7213 bp
  85% of sequence in fragments shorter than    8396 bp
  90% of sequence in fragments shorter than   10142 bp
  95% of sequence in fragments shorter than   13808 bp

# BsmI.PmeI (GAATGC+GTTTAAAC) double digest, 1590 thousand fragments (1549350+42094 sites), 1873 bp average fragment length
   5% of sequence in fragments shorter than     665 bp
  10% of sequence in fragments shorter than    1125 bp
  15% of sequence in fragments shorter than    1513 bp
  20% of sequence in fragments shorter than    1867 bp
  25% of sequence in fragments shorter than    2207 bp
  30% of sequence in fragments shorter than    2534 bp
  35% of sequence in fragments shorter than    2865 bp
  40% of sequence in fragments shorter than    3207 bp
  45% of sequence in fragments shorter than    3570 bp
  50% of sequence in fragments shorter than    3952 bp
  55% of sequence in fragments shorter than    4359 bp
  60% of sequence in fragments shorter than    4801 bp
  65% of sequence in fragments shorter than    5290 bp
  70% of sequence in fragments shorter than    5841 bp
  75% of sequence in fragments shorter than    6475 bp
  80% of sequence in fragments shorter than    7224 bp
  85% of sequence in fragments shorter than    8179 bp
  90% of sequence in fragments shorter than    9497 bp
  95% of sequence in fragments shorter than   11691 bp

# BclI.XbaI (TGATCA+TCTAGA) double digest, 1589 thousand fragments (738785+850998 sites), 1875 bp average fragment length
   5% of sequence in fragments shorter than     647 bp
  10% of sequence in fragments shorter than     988 bp
  15% of sequence in fragments shorter than    1279 bp
  20% of sequence in fragments shorter than    1539 bp
  25% of sequence in fragments shorter than    1807 bp
  30% of sequence in fragments shorter than    2065 bp
  35% of sequence in fragments shorter than    2334 bp
  40% of sequence in fragments shorter than    2619 bp
  45% of sequence in fragments shorter than    2919 bp
  50% of sequence in fragments shorter than    3236 bp
  55% of sequence in fragments shorter than    3573 bp
  60% of sequence in fragments shorter than    3943 bp
  65% of sequence in fragments shorter than    4354 bp
  70% of sequence in fragments shorter than    4817 bp
  75% of sequence in fragments shorter than    5356 bp
  80% of sequence in fragments shorter than    6010 bp
  85% of sequence in fragments shorter than    6862 bp
  90% of sequence in fragments shorter than    8062 bp
  95% of sequence in fragments shorter than   10308 bp

# BclI.EcoRI (TGATCA+GAATTC) double digest, 1585 thousand fragments (738785+847401 sites), 1879 bp average fragment length
   5% of sequence in fragments shorter than     649 bp
  10% of sequence in fragments shorter than     980 bp
  15% of sequence in fragments shorter than    1290 bp
  20% of sequence in fragments shorter than    1572 bp
  25% of sequence in fragments shorter than    1848 bp
  30% of sequence in fragments shorter than    2121 bp
  35% of sequence in fragments shorter than    2400 bp
  40% of sequence in fragments shorter than    2682 bp
  45% of sequence in fragments shorter than    2976 bp
  50% of sequence in fragments shorter than    3295 bp
  55% of sequence in fragments shorter than    3633 bp
  60% of sequence in fragments shorter than    3999 bp
  65% of sequence in fragments shorter than    4407 bp
  70% of sequence in fragments shorter than    4871 bp
  75% of sequence in fragments shorter than    5416 bp
  80% of sequence in fragments shorter than    6065 bp
  85% of sequence in fragments shorter than    6908 bp
  90% of sequence in fragments shorter than    8114 bp
  95% of sequence in fragments shorter than   10333 bp

# FnuDII.XbaI (CGCG+TCTAGA) double digest, 1584 thousand fragments (733940+850998 sites), 1880 bp average fragment length
   5% of sequence in fragments shorter than     718 bp
  10% of sequence in fragments shorter than    1135 bp
  15% of sequence in fragments shorter than    1492 bp
  20% of sequence in fragments shorter than    1845 bp
  25% of sequence in fragments shorter than    2187 bp
  30% of sequence in fragments shorter than    2516 bp
  35% of sequence in fragments shorter than    2864 bp
  40% of sequence in fragments shorter than    3209 bp
  45% of sequence in fragments shorter than    3581 bp
  50% of sequence in fragments shorter than    3982 bp
  55% of sequence in fragments shorter than    4407 bp
  60% of sequence in fragments shorter than    4867 bp
  65% of sequence in fragments shorter than    5372 bp
  70% of sequence in fragments shorter than    5945 bp
  75% of sequence in fragments shorter than    6610 bp
  80% of sequence in fragments shorter than    7418 bp
  85% of sequence in fragments shorter than    8428 bp
  90% of sequence in fragments shorter than    9848 bp
  95% of sequence in fragments shorter than   12332 bp

# HgiCI.SalI (GGYRCC+GTCGAC) double digest, 1581 thousand fragments (1550877+31396 sites), 1883 bp average fragment length
   5% of sequence in fragments shorter than     618 bp
  10% of sequence in fragments shorter than     973 bp
  15% of sequence in fragments shorter than    1268 bp
  20% of sequence in fragments shorter than    1576 bp
  25% of sequence in fragments shorter than    1876 bp
  30% of sequence in fragments shorter than    2194 bp
  35% of sequence in fragments shorter than    2526 bp
  40% of sequence in fragments shorter than    2860 bp
  45% of sequence in fragments shorter than    3217 bp
  50% of sequence in fragments shorter than    3616 bp
  55% of sequence in fragments shorter than    4053 bp
  60% of sequence in fragments shorter than    4528 bp
  65% of sequence in fragments shorter than    5075 bp
  70% of sequence in fragments shorter than    5702 bp
  75% of sequence in fragments shorter than    6454 bp
  80% of sequence in fragments shorter than    7392 bp
  85% of sequence in fragments shorter than    8616 bp
  90% of sequence in fragments shorter than   10445 bp
  95% of sequence in fragments shorter than   14265 bp

# BspHI.SacI (TCATGA+GAGCTC) double digest, 1581 thousand fragments (978290+603323 sites), 1884 bp average fragment length
   5% of sequence in fragments shorter than     671 bp
  10% of sequence in fragments shorter than    1010 bp
  15% of sequence in fragments shorter than    1305 bp
  20% of sequence in fragments shorter than    1581 bp
  25% of sequence in fragments shorter than    1849 bp
  30% of sequence in fragments shorter than    2103 bp
  35% of sequence in fragments shorter than    2369 bp
  40% of sequence in fragments shorter than    2618 bp
  45% of sequence in fragments shorter than    2906 bp
  50% of sequence in fragments shorter than    3207 bp
  55% of sequence in fragments shorter than    3530 bp
  60% of sequence in fragments shorter than    3886 bp
  65% of sequence in fragments shorter than    4279 bp
  70% of sequence in fragments shorter than    4727 bp
  75% of sequence in fragments shorter than    5247 bp
  80% of sequence in fragments shorter than    5884 bp
  85% of sequence in fragments shorter than    6696 bp
  90% of sequence in fragments shorter than    7881 bp
  95% of sequence in fragments shorter than   10075 bp

# ApaLI.PpuMI (GTGCAC+RGGWCCY) double digest, 1580 thousand fragments (496255+1085138 sites), 1884 bp average fragment length
   5% of sequence in fragments shorter than     620 bp
  10% of sequence in fragments shorter than     987 bp
  15% of sequence in fragments shorter than    1318 bp
  20% of sequence in fragments shorter than    1638 bp
  25% of sequence in fragments shorter than    1924 bp
  30% of sequence in fragments shorter than    2247 bp
  35% of sequence in fragments shorter than    2586 bp
  40% of sequence in fragments shorter than    2940 bp
  45% of sequence in fragments shorter than    3308 bp
  50% of sequence in fragments shorter than    3670 bp
  55% of sequence in fragments shorter than    4090 bp
  60% of sequence in fragments shorter than    4567 bp
  65% of sequence in fragments shorter than    5101 bp
  70% of sequence in fragments shorter than    5710 bp
  75% of sequence in fragments shorter than    6435 bp
  80% of sequence in fragments shorter than    7327 bp
  85% of sequence in fragments shorter than    8501 bp
  90% of sequence in fragments shorter than   10251 bp
  95% of sequence in fragments shorter than   13638 bp

# EcoRI.FnuDII (GAATTC+CGCG) double digest, 1580 thousand fragments (847401+733940 sites), 1885 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1141 bp
  15% of sequence in fragments shorter than    1536 bp
  20% of sequence in fragments shorter than    1906 bp
  25% of sequence in fragments shorter than    2263 bp
  30% of sequence in fragments shorter than    2617 bp
  35% of sequence in fragments shorter than    2972 bp
  40% of sequence in fragments shorter than    3329 bp
  45% of sequence in fragments shorter than    3709 bp
  50% of sequence in fragments shorter than    4122 bp
  55% of sequence in fragments shorter than    4559 bp
  60% of sequence in fragments shorter than    5043 bp
  65% of sequence in fragments shorter than    5569 bp
  70% of sequence in fragments shorter than    6171 bp
  75% of sequence in fragments shorter than    6870 bp
  80% of sequence in fragments shorter than    7706 bp
  85% of sequence in fragments shorter than    8759 bp
  90% of sequence in fragments shorter than   10257 bp
  95% of sequence in fragments shorter than   12934 bp

# BsmI.SalI (GAATGC+GTCGAC) double digest, 1580 thousand fragments (1549350+31396 sites), 1885 bp average fragment length
   5% of sequence in fragments shorter than     671 bp
  10% of sequence in fragments shorter than    1136 bp
  15% of sequence in fragments shorter than    1529 bp
  20% of sequence in fragments shorter than    1885 bp
  25% of sequence in fragments shorter than    2229 bp
  30% of sequence in fragments shorter than    2559 bp
  35% of sequence in fragments shorter than    2892 bp
  40% of sequence in fragments shorter than    3239 bp
  45% of sequence in fragments shorter than    3604 bp
  50% of sequence in fragments shorter than    3986 bp
  55% of sequence in fragments shorter than    4399 bp
  60% of sequence in fragments shorter than    4843 bp
  65% of sequence in fragments shorter than    5341 bp
  70% of sequence in fragments shorter than    5893 bp
  75% of sequence in fragments shorter than    6534 bp
  80% of sequence in fragments shorter than    7287 bp
  85% of sequence in fragments shorter than    8255 bp
  90% of sequence in fragments shorter than    9577 bp
  95% of sequence in fragments shorter than   11782 bp

# ApaLI.PvuII (GTGCAC+CAGCTG) double digest, 1580 thousand fragments (496255+1084260 sites), 1885 bp average fragment length
   5% of sequence in fragments shorter than     639 bp
  10% of sequence in fragments shorter than     995 bp
  15% of sequence in fragments shorter than    1310 bp
  20% of sequence in fragments shorter than    1608 bp
  25% of sequence in fragments shorter than    1908 bp
  30% of sequence in fragments shorter than    2215 bp
  35% of sequence in fragments shorter than    2528 bp
  40% of sequence in fragments shorter than    2842 bp
  45% of sequence in fragments shorter than    3183 bp
  50% of sequence in fragments shorter than    3552 bp
  55% of sequence in fragments shorter than    3956 bp
  60% of sequence in fragments shorter than    4391 bp
  65% of sequence in fragments shorter than    4886 bp
  70% of sequence in fragments shorter than    5407 bp
  75% of sequence in fragments shorter than    6061 bp
  80% of sequence in fragments shorter than    6875 bp
  85% of sequence in fragments shorter than    7913 bp
  90% of sequence in fragments shorter than    9434 bp
  95% of sequence in fragments shorter than   12403 bp

# AvaI.HgaI (CYCGRG+GACGC) double digest, 1577 thousand fragments (1005714+571889 sites), 1889 bp average fragment length
   5% of sequence in fragments shorter than     605 bp
  10% of sequence in fragments shorter than    1022 bp
  15% of sequence in fragments shorter than    1412 bp
  20% of sequence in fragments shorter than    1809 bp
  25% of sequence in fragments shorter than    2222 bp
  30% of sequence in fragments shorter than    2660 bp
  35% of sequence in fragments shorter than    3121 bp
  40% of sequence in fragments shorter than    3624 bp
  45% of sequence in fragments shorter than    4164 bp
  50% of sequence in fragments shorter than    4761 bp
  55% of sequence in fragments shorter than    5418 bp
  60% of sequence in fragments shorter than    6153 bp
  65% of sequence in fragments shorter than    6995 bp
  70% of sequence in fragments shorter than    7976 bp
  75% of sequence in fragments shorter than    9153 bp
  80% of sequence in fragments shorter than   10630 bp
  85% of sequence in fragments shorter than   12542 bp
  90% of sequence in fragments shorter than   15374 bp
  95% of sequence in fragments shorter than   20749 bp

# AvaI.EciI (CYCGRG+GGCGGA) double digest, 1576 thousand fragments (1005714+571273 sites), 1890 bp average fragment length
   5% of sequence in fragments shorter than     627 bp
  10% of sequence in fragments shorter than    1073 bp
  15% of sequence in fragments shorter than    1499 bp
  20% of sequence in fragments shorter than    1938 bp
  25% of sequence in fragments shorter than    2395 bp
  30% of sequence in fragments shorter than    2891 bp
  35% of sequence in fragments shorter than    3420 bp
  40% of sequence in fragments shorter than    4001 bp
  45% of sequence in fragments shorter than    4639 bp
  50% of sequence in fragments shorter than    5346 bp
  55% of sequence in fragments shorter than    6132 bp
  60% of sequence in fragments shorter than    7020 bp
  65% of sequence in fragments shorter than    8037 bp
  70% of sequence in fragments shorter than    9207 bp
  75% of sequence in fragments shorter than   10651 bp
  80% of sequence in fragments shorter than   12440 bp
  85% of sequence in fragments shorter than   14847 bp
  90% of sequence in fragments shorter than   18432 bp
  95% of sequence in fragments shorter than   25795 bp

# AflII.AvaIII (CTTAAG+ATGCAT) double digest, 1576 thousand fragments (643284+933357 sites), 1890 bp average fragment length
   5% of sequence in fragments shorter than     664 bp
  10% of sequence in fragments shorter than    1007 bp
  15% of sequence in fragments shorter than    1307 bp
  20% of sequence in fragments shorter than    1588 bp
  25% of sequence in fragments shorter than    1862 bp
  30% of sequence in fragments shorter than    2130 bp
  35% of sequence in fragments shorter than    2408 bp
  40% of sequence in fragments shorter than    2696 bp
  45% of sequence in fragments shorter than    2997 bp
  50% of sequence in fragments shorter than    3324 bp
  55% of sequence in fragments shorter than    3675 bp
  60% of sequence in fragments shorter than    4062 bp
  65% of sequence in fragments shorter than    4492 bp
  70% of sequence in fragments shorter than    4974 bp
  75% of sequence in fragments shorter than    5544 bp
  80% of sequence in fragments shorter than    6265 bp
  85% of sequence in fragments shorter than    7219 bp
  90% of sequence in fragments shorter than    8669 bp
  95% of sequence in fragments shorter than   11678 bp

# BsaAI.HindIII (YACGTR+AAGCTT) double digest, 1573 thousand fragments (713801+860365 sites), 1893 bp average fragment length
   5% of sequence in fragments shorter than     669 bp
  10% of sequence in fragments shorter than    1023 bp
  15% of sequence in fragments shorter than    1314 bp
  20% of sequence in fragments shorter than    1577 bp
  25% of sequence in fragments shorter than    1849 bp
  30% of sequence in fragments shorter than    2082 bp
  35% of sequence in fragments shorter than    2355 bp
  40% of sequence in fragments shorter than    2639 bp
  45% of sequence in fragments shorter than    2929 bp
  50% of sequence in fragments shorter than    3240 bp
  55% of sequence in fragments shorter than    3575 bp
  60% of sequence in fragments shorter than    3936 bp
  65% of sequence in fragments shorter than    4339 bp
  70% of sequence in fragments shorter than    4793 bp
  75% of sequence in fragments shorter than    5319 bp
  80% of sequence in fragments shorter than    5948 bp
  85% of sequence in fragments shorter than    6753 bp
  90% of sequence in fragments shorter than    7874 bp
  95% of sequence in fragments shorter than    9870 bp

# AvrII.BspHI (CCTAGG+TCATGA) double digest, 1572 thousand fragments (594956+978290 sites), 1894 bp average fragment length
   5% of sequence in fragments shorter than     662 bp
  10% of sequence in fragments shorter than    1007 bp
  15% of sequence in fragments shorter than    1305 bp
  20% of sequence in fragments shorter than    1587 bp
  25% of sequence in fragments shorter than    1860 bp
  30% of sequence in fragments shorter than    2125 bp
  35% of sequence in fragments shorter than    2396 bp
  40% of sequence in fragments shorter than    2680 bp
  45% of sequence in fragments shorter than    2977 bp
  50% of sequence in fragments shorter than    3290 bp
  55% of sequence in fragments shorter than    3626 bp
  60% of sequence in fragments shorter than    3996 bp
  65% of sequence in fragments shorter than    4400 bp
  70% of sequence in fragments shorter than    4850 bp
  75% of sequence in fragments shorter than    5378 bp
  80% of sequence in fragments shorter than    6013 bp
  85% of sequence in fragments shorter than    6833 bp
  90% of sequence in fragments shorter than    8033 bp
  95% of sequence in fragments shorter than   10349 bp

# HgiCI.MluI (GGYRCC+ACGCGT) double digest, 1571 thousand fragments (1550877+21415 sites), 1895 bp average fragment length
   5% of sequence in fragments shorter than     621 bp
  10% of sequence in fragments shorter than     978 bp
  15% of sequence in fragments shorter than    1276 bp
  20% of sequence in fragments shorter than    1582 bp
  25% of sequence in fragments shorter than    1891 bp
  30% of sequence in fragments shorter than    2212 bp
  35% of sequence in fragments shorter than    2547 bp
  40% of sequence in fragments shorter than    2882 bp
  45% of sequence in fragments shorter than    3247 bp
  50% of sequence in fragments shorter than    3650 bp
  55% of sequence in fragments shorter than    4089 bp
  60% of sequence in fragments shorter than    4574 bp
  65% of sequence in fragments shorter than    5129 bp
  70% of sequence in fragments shorter than    5766 bp
  75% of sequence in fragments shorter than    6529 bp
  80% of sequence in fragments shorter than    7482 bp
  85% of sequence in fragments shorter than    8730 bp
  90% of sequence in fragments shorter than   10582 bp
  95% of sequence in fragments shorter than   14527 bp

# FauI.SnaBI (CCCGC+TACGTA) double digest, 1571 thousand fragments (1439772+132299 sites), 1896 bp average fragment length
   5% of sequence in fragments shorter than     665 bp
  10% of sequence in fragments shorter than    1155 bp
  15% of sequence in fragments shorter than    1596 bp
  20% of sequence in fragments shorter than    2054 bp
  25% of sequence in fragments shorter than    2515 bp
  30% of sequence in fragments shorter than    2995 bp
  35% of sequence in fragments shorter than    3507 bp
  40% of sequence in fragments shorter than    4055 bp
  45% of sequence in fragments shorter than    4614 bp
  50% of sequence in fragments shorter than    5255 bp
  55% of sequence in fragments shorter than    5951 bp
  60% of sequence in fragments shorter than    6734 bp
  65% of sequence in fragments shorter than    7612 bp
  70% of sequence in fragments shorter than    8623 bp
  75% of sequence in fragments shorter than    9838 bp
  80% of sequence in fragments shorter than   11317 bp
  85% of sequence in fragments shorter than   13229 bp
  90% of sequence in fragments shorter than   16052 bp
  95% of sequence in fragments shorter than   21188 bp

# AflIII.ClaI (ACRYGT+ATCGAT) double digest, 1571 thousand fragments (1485394+86477 sites), 1896 bp average fragment length
   5% of sequence in fragments shorter than     686 bp
  10% of sequence in fragments shorter than    1065 bp
  15% of sequence in fragments shorter than    1389 bp
  20% of sequence in fragments shorter than    1694 bp
  25% of sequence in fragments shorter than    1982 bp
  30% of sequence in fragments shorter than    2262 bp
  35% of sequence in fragments shorter than    2558 bp
  40% of sequence in fragments shorter than    2860 bp
  45% of sequence in fragments shorter than    3176 bp
  50% of sequence in fragments shorter than    3516 bp
  55% of sequence in fragments shorter than    3880 bp
  60% of sequence in fragments shorter than    4280 bp
  65% of sequence in fragments shorter than    4721 bp
  70% of sequence in fragments shorter than    5225 bp
  75% of sequence in fragments shorter than    5808 bp
  80% of sequence in fragments shorter than    6501 bp
  85% of sequence in fragments shorter than    7395 bp
  90% of sequence in fragments shorter than    8677 bp
  95% of sequence in fragments shorter than   11081 bp

# BsmI.MluI (GAATGC+ACGCGT) double digest, 1570 thousand fragments (1549350+21415 sites), 1897 bp average fragment length
   5% of sequence in fragments shorter than     678 bp
  10% of sequence in fragments shorter than    1146 bp
  15% of sequence in fragments shorter than    1542 bp
  20% of sequence in fragments shorter than    1901 bp
  25% of sequence in fragments shorter than    2245 bp
  30% of sequence in fragments shorter than    2577 bp
  35% of sequence in fragments shorter than    2914 bp
  40% of sequence in fragments shorter than    3263 bp
  45% of sequence in fragments shorter than    3632 bp
  50% of sequence in fragments shorter than    4019 bp
  55% of sequence in fragments shorter than    4434 bp
  60% of sequence in fragments shorter than    4883 bp
  65% of sequence in fragments shorter than    5381 bp
  70% of sequence in fragments shorter than    5937 bp
  75% of sequence in fragments shorter than    6580 bp
  80% of sequence in fragments shorter than    7335 bp
  85% of sequence in fragments shorter than    8303 bp
  90% of sequence in fragments shorter than    9640 bp
  95% of sequence in fragments shorter than   11859 bp

# BspMI.SnaBI (ACCTGC+TACGTA) double digest, 1569 thousand fragments (1438129+132299 sites), 1898 bp average fragment length
   5% of sequence in fragments shorter than     648 bp
  10% of sequence in fragments shorter than     989 bp
  15% of sequence in fragments shorter than    1311 bp
  20% of sequence in fragments shorter than    1610 bp
  25% of sequence in fragments shorter than    1913 bp
  30% of sequence in fragments shorter than    2186 bp
  35% of sequence in fragments shorter than    2491 bp
  40% of sequence in fragments shorter than    2804 bp
  45% of sequence in fragments shorter than    3138 bp
  50% of sequence in fragments shorter than    3447 bp
  55% of sequence in fragments shorter than    3828 bp
  60% of sequence in fragments shorter than    4246 bp
  65% of sequence in fragments shorter than    4708 bp
  70% of sequence in fragments shorter than    5237 bp
  75% of sequence in fragments shorter than    5844 bp
  80% of sequence in fragments shorter than    6577 bp
  85% of sequence in fragments shorter than    7525 bp
  90% of sequence in fragments shorter than    8852 bp
  95% of sequence in fragments shorter than   11137 bp

# AvaI.MfeI (CYCGRG+CAATTG) double digest, 1569 thousand fragments (1005714+564303 sites), 1898 bp average fragment length
   5% of sequence in fragments shorter than     653 bp
  10% of sequence in fragments shorter than    1015 bp
  15% of sequence in fragments shorter than    1341 bp
  20% of sequence in fragments shorter than    1657 bp
  25% of sequence in fragments shorter than    1962 bp
  30% of sequence in fragments shorter than    2267 bp
  35% of sequence in fragments shorter than    2587 bp
  40% of sequence in fragments shorter than    2898 bp
  45% of sequence in fragments shorter than    3242 bp
  50% of sequence in fragments shorter than    3618 bp
  55% of sequence in fragments shorter than    4017 bp
  60% of sequence in fragments shorter than    4451 bp
  65% of sequence in fragments shorter than    4938 bp
  70% of sequence in fragments shorter than    5496 bp
  75% of sequence in fragments shorter than    6150 bp
  80% of sequence in fragments shorter than    6957 bp
  85% of sequence in fragments shorter than    7990 bp
  90% of sequence in fragments shorter than    9475 bp
  95% of sequence in fragments shorter than   12270 bp

# AflII.StuI (CTTAAG+AGGCCT) double digest, 1568 thousand fragments (643284+925729 sites), 1899 bp average fragment length
   5% of sequence in fragments shorter than     633 bp
  10% of sequence in fragments shorter than     974 bp
  15% of sequence in fragments shorter than    1306 bp
  20% of sequence in fragments shorter than    1597 bp
  25% of sequence in fragments shorter than    1902 bp
  30% of sequence in fragments shorter than    2184 bp
  35% of sequence in fragments shorter than    2480 bp
  40% of sequence in fragments shorter than    2792 bp
  45% of sequence in fragments shorter than    3121 bp
  50% of sequence in fragments shorter than    3466 bp
  55% of sequence in fragments shorter than    3834 bp
  60% of sequence in fragments shorter than    4232 bp
  65% of sequence in fragments shorter than    4672 bp
  70% of sequence in fragments shorter than    5169 bp
  75% of sequence in fragments shorter than    5740 bp
  80% of sequence in fragments shorter than    6431 bp
  85% of sequence in fragments shorter than    7301 bp
  90% of sequence in fragments shorter than    8495 bp
  95% of sequence in fragments shorter than   10522 bp

# BscGI.BstEII (CCCGT+GGTNACC) double digest, 1566 thousand fragments (1231070+335865 sites), 1902 bp average fragment length
   5% of sequence in fragments shorter than     554 bp
  10% of sequence in fragments shorter than     944 bp
  15% of sequence in fragments shorter than    1315 bp
  20% of sequence in fragments shorter than    1676 bp
  25% of sequence in fragments shorter than    2040 bp
  30% of sequence in fragments shorter than    2416 bp
  35% of sequence in fragments shorter than    2804 bp
  40% of sequence in fragments shorter than    3214 bp
  45% of sequence in fragments shorter than    3647 bp
  50% of sequence in fragments shorter than    4119 bp
  55% of sequence in fragments shorter than    4624 bp
  60% of sequence in fragments shorter than    5182 bp
  65% of sequence in fragments shorter than    5812 bp
  70% of sequence in fragments shorter than    6527 bp
  75% of sequence in fragments shorter than    7363 bp
  80% of sequence in fragments shorter than    8392 bp
  85% of sequence in fragments shorter than    9720 bp
  90% of sequence in fragments shorter than   11570 bp
  95% of sequence in fragments shorter than   14807 bp

# HgiCI.SgrAI (GGYRCC+CRCCGGYG) double digest, 1565 thousand fragments (1550877+15270 sites), 1903 bp average fragment length
   5% of sequence in fragments shorter than     626 bp
  10% of sequence in fragments shorter than     986 bp
  15% of sequence in fragments shorter than    1285 bp
  20% of sequence in fragments shorter than    1587 bp
  25% of sequence in fragments shorter than    1905 bp
  30% of sequence in fragments shorter than    2229 bp
  35% of sequence in fragments shorter than    2566 bp
  40% of sequence in fragments shorter than    2900 bp
  45% of sequence in fragments shorter than    3270 bp
  50% of sequence in fragments shorter than    3677 bp
  55% of sequence in fragments shorter than    4118 bp
  60% of sequence in fragments shorter than    4606 bp
  65% of sequence in fragments shorter than    5166 bp
  70% of sequence in fragments shorter than    5806 bp
  75% of sequence in fragments shorter than    6573 bp
  80% of sequence in fragments shorter than    7534 bp
  85% of sequence in fragments shorter than    8785 bp
  90% of sequence in fragments shorter than   10655 bp
  95% of sequence in fragments shorter than   14640 bp

# HgiCI.NruI (GGYRCC+TCGCGA) double digest, 1565 thousand fragments (1550877+14872 sites), 1903 bp average fragment length
   5% of sequence in fragments shorter than     625 bp
  10% of sequence in fragments shorter than     984 bp
  15% of sequence in fragments shorter than    1282 bp
  20% of sequence in fragments shorter than    1584 bp
  25% of sequence in fragments shorter than    1900 bp
  30% of sequence in fragments shorter than    2223 bp
  35% of sequence in fragments shorter than    2558 bp
  40% of sequence in fragments shorter than    2892 bp
  45% of sequence in fragments shorter than    3260 bp
  50% of sequence in fragments shorter than    3664 bp
  55% of sequence in fragments shorter than    4104 bp
  60% of sequence in fragments shorter than    4590 bp
  65% of sequence in fragments shorter than    5149 bp
  70% of sequence in fragments shorter than    5786 bp
  75% of sequence in fragments shorter than    6552 bp
  80% of sequence in fragments shorter than    7510 bp
  85% of sequence in fragments shorter than    8759 bp
  90% of sequence in fragments shorter than   10612 bp
  95% of sequence in fragments shorter than   14553 bp

# FauI.NaeI (CCCGC+GCCGGC) double digest, 1565 thousand fragments (1439772+125692 sites), 1904 bp average fragment length
   5% of sequence in fragments shorter than     662 bp
  10% of sequence in fragments shorter than    1187 bp
  15% of sequence in fragments shorter than    1684 bp
  20% of sequence in fragments shorter than    2190 bp
  25% of sequence in fragments shorter than    2731 bp
  30% of sequence in fragments shorter than    3298 bp
  35% of sequence in fragments shorter than    3916 bp
  40% of sequence in fragments shorter than    4543 bp
  45% of sequence in fragments shorter than    5263 bp
  50% of sequence in fragments shorter than    6056 bp
  55% of sequence in fragments shorter than    6932 bp
  60% of sequence in fragments shorter than    7923 bp
  65% of sequence in fragments shorter than    9042 bp
  70% of sequence in fragments shorter than   10347 bp
  75% of sequence in fragments shorter than   11889 bp
  80% of sequence in fragments shorter than   13780 bp
  85% of sequence in fragments shorter than   16343 bp
  90% of sequence in fragments shorter than   20084 bp
  95% of sequence in fragments shorter than   27393 bp

# BceAI.HindIII (ACGGC+AAGCTT) double digest, 1565 thousand fragments (705178+860365 sites), 1904 bp average fragment length
   5% of sequence in fragments shorter than     652 bp
  10% of sequence in fragments shorter than    1012 bp
  15% of sequence in fragments shorter than    1322 bp
  20% of sequence in fragments shorter than    1615 bp
  25% of sequence in fragments shorter than    1902 bp
  30% of sequence in fragments shorter than    2173 bp
  35% of sequence in fragments shorter than    2469 bp
  40% of sequence in fragments shorter than    2771 bp
  45% of sequence in fragments shorter than    3088 bp
  50% of sequence in fragments shorter than    3427 bp
  55% of sequence in fragments shorter than    3792 bp
  60% of sequence in fragments shorter than    4185 bp
  65% of sequence in fragments shorter than    4620 bp
  70% of sequence in fragments shorter than    5119 bp
  75% of sequence in fragments shorter than    5694 bp
  80% of sequence in fragments shorter than    6392 bp
  85% of sequence in fragments shorter than    7284 bp
  90% of sequence in fragments shorter than    8539 bp
  95% of sequence in fragments shorter than   10805 bp

# HgiCI.PvuI (GGYRCC+CGATCG) double digest, 1564 thousand fragments (1550877+14186 sites), 1904 bp average fragment length
   5% of sequence in fragments shorter than     626 bp
  10% of sequence in fragments shorter than     985 bp
  15% of sequence in fragments shorter than    1282 bp
  20% of sequence in fragments shorter than    1584 bp
  25% of sequence in fragments shorter than    1897 bp
  30% of sequence in fragments shorter than    2219 bp
  35% of sequence in fragments shorter than    2554 bp
  40% of sequence in fragments shorter than    2888 bp
  45% of sequence in fragments shorter than    3255 bp
  50% of sequence in fragments shorter than    3658 bp
  55% of sequence in fragments shorter than    4099 bp
  60% of sequence in fragments shorter than    4583 bp
  65% of sequence in fragments shorter than    5139 bp
  70% of sequence in fragments shorter than    5776 bp
  75% of sequence in fragments shorter than    6537 bp
  80% of sequence in fragments shorter than    7489 bp
  85% of sequence in fragments shorter than    8730 bp
  90% of sequence in fragments shorter than   10566 bp
  95% of sequence in fragments shorter than   14387 bp

# BsaAI.XbaI (YACGTR+TCTAGA) double digest, 1564 thousand fragments (713801+850998 sites), 1904 bp average fragment length
   5% of sequence in fragments shorter than     684 bp
  10% of sequence in fragments shorter than    1019 bp
  15% of sequence in fragments shorter than    1316 bp
  20% of sequence in fragments shorter than    1587 bp
  25% of sequence in fragments shorter than    1859 bp
  30% of sequence in fragments shorter than    2123 bp
  35% of sequence in fragments shorter than    2397 bp
  40% of sequence in fragments shorter than    2688 bp
  45% of sequence in fragments shorter than    2989 bp
  50% of sequence in fragments shorter than    3310 bp
  55% of sequence in fragments shorter than    3649 bp
  60% of sequence in fragments shorter than    4022 bp
  65% of sequence in fragments shorter than    4433 bp
  70% of sequence in fragments shorter than    4891 bp
  75% of sequence in fragments shorter than    5421 bp
  80% of sequence in fragments shorter than    6064 bp
  85% of sequence in fragments shorter than    6861 bp
  90% of sequence in fragments shorter than    7968 bp
  95% of sequence in fragments shorter than    9868 bp

# FseI.HgiCI (GGCCGGCC+GGYRCC) double digest, 1564 thousand fragments (13662+1550877 sites), 1905 bp average fragment length
   5% of sequence in fragments shorter than     625 bp
  10% of sequence in fragments shorter than     985 bp
  15% of sequence in fragments shorter than    1284 bp
  20% of sequence in fragments shorter than    1586 bp
  25% of sequence in fragments shorter than    1904 bp
  30% of sequence in fragments shorter than    2228 bp
  35% of sequence in fragments shorter than    2566 bp
  40% of sequence in fragments shorter than    2901 bp
  45% of sequence in fragments shorter than    3271 bp
  50% of sequence in fragments shorter than    3678 bp
  55% of sequence in fragments shorter than    4120 bp
  60% of sequence in fragments shorter than    4608 bp
  65% of sequence in fragments shorter than    5169 bp
  70% of sequence in fragments shorter than    5809 bp
  75% of sequence in fragments shorter than    6576 bp
  80% of sequence in fragments shorter than    7539 bp
  85% of sequence in fragments shorter than    8794 bp
  90% of sequence in fragments shorter than   10668 bp
  95% of sequence in fragments shorter than   14654 bp

# BsmI.SgrAI (GAATGC+CRCCGGYG) double digest, 1564 thousand fragments (1549350+15270 sites), 1905 bp average fragment length
   5% of sequence in fragments shorter than     681 bp
  10% of sequence in fragments shorter than    1152 bp
  15% of sequence in fragments shorter than    1550 bp
  20% of sequence in fragments shorter than    1910 bp
  25% of sequence in fragments shorter than    2257 bp
  30% of sequence in fragments shorter than    2591 bp
  35% of sequence in fragments shorter than    2929 bp
  40% of sequence in fragments shorter than    3280 bp
  45% of sequence in fragments shorter than    3651 bp
  50% of sequence in fragments shorter than    4040 bp
  55% of sequence in fragments shorter than    4457 bp
  60% of sequence in fragments shorter than    4907 bp
  65% of sequence in fragments shorter than    5407 bp
  70% of sequence in fragments shorter than    5964 bp
  75% of sequence in fragments shorter than    6612 bp
  80% of sequence in fragments shorter than    7372 bp
  85% of sequence in fragments shorter than    8346 bp
  90% of sequence in fragments shorter than    9689 bp
  95% of sequence in fragments shorter than   11917 bp

# BsmI.NruI (GAATGC+TCGCGA) double digest, 1563 thousand fragments (1549350+14872 sites), 1905 bp average fragment length
   5% of sequence in fragments shorter than     681 bp
  10% of sequence in fragments shorter than    1152 bp
  15% of sequence in fragments shorter than    1550 bp
  20% of sequence in fragments shorter than    1909 bp
  25% of sequence in fragments shorter than    2257 bp
  30% of sequence in fragments shorter than    2591 bp
  35% of sequence in fragments shorter than    2929 bp
  40% of sequence in fragments shorter than    3280 bp
  45% of sequence in fragments shorter than    3650 bp
  50% of sequence in fragments shorter than    4039 bp
  55% of sequence in fragments shorter than    4455 bp
  60% of sequence in fragments shorter than    4905 bp
  65% of sequence in fragments shorter than    5405 bp
  70% of sequence in fragments shorter than    5964 bp
  75% of sequence in fragments shorter than    6612 bp
  80% of sequence in fragments shorter than    7374 bp
  85% of sequence in fragments shorter than    8348 bp
  90% of sequence in fragments shorter than    9690 bp
  95% of sequence in fragments shorter than   11929 bp

# BspMI.NaeI (ACCTGC+GCCGGC) double digest, 1563 thousand fragments (1438129+125692 sites), 1906 bp average fragment length
   5% of sequence in fragments shorter than     638 bp
  10% of sequence in fragments shorter than     994 bp
  15% of sequence in fragments shorter than    1330 bp
  20% of sequence in fragments shorter than    1643 bp
  25% of sequence in fragments shorter than    1962 bp
  30% of sequence in fragments shorter than    2256 bp
  35% of sequence in fragments shorter than    2586 bp
  40% of sequence in fragments shorter than    2925 bp
  45% of sequence in fragments shorter than    3271 bp
  50% of sequence in fragments shorter than    3625 bp
  55% of sequence in fragments shorter than    4047 bp
  60% of sequence in fragments shorter than    4508 bp
  65% of sequence in fragments shorter than    5018 bp
  70% of sequence in fragments shorter than    5599 bp
  75% of sequence in fragments shorter than    6280 bp
  80% of sequence in fragments shorter than    7105 bp
  85% of sequence in fragments shorter than    8178 bp
  90% of sequence in fragments shorter than    9666 bp
  95% of sequence in fragments shorter than   12318 bp

# BsmI.PvuI (GAATGC+CGATCG) double digest, 1563 thousand fragments (1549350+14186 sites), 1906 bp average fragment length
   5% of sequence in fragments shorter than     682 bp
  10% of sequence in fragments shorter than    1154 bp
  15% of sequence in fragments shorter than    1553 bp
  20% of sequence in fragments shorter than    1913 bp
  25% of sequence in fragments shorter than    2260 bp
  30% of sequence in fragments shorter than    2596 bp
  35% of sequence in fragments shorter than    2935 bp
  40% of sequence in fragments shorter than    3287 bp
  45% of sequence in fragments shorter than    3659 bp
  50% of sequence in fragments shorter than    4050 bp
  55% of sequence in fragments shorter than    4467 bp
  60% of sequence in fragments shorter than    4919 bp
  65% of sequence in fragments shorter than    5422 bp
  70% of sequence in fragments shorter than    5981 bp
  75% of sequence in fragments shorter than    6633 bp
  80% of sequence in fragments shorter than    7396 bp
  85% of sequence in fragments shorter than    8379 bp
  90% of sequence in fragments shorter than    9727 bp
  95% of sequence in fragments shorter than   11963 bp

# AvaIII.HaeII (ATGCAT+RGCGCY) double digest, 1562 thousand fragments (933357+630101 sites), 1906 bp average fragment length
   5% of sequence in fragments shorter than     658 bp
  10% of sequence in fragments shorter than    1011 bp
  15% of sequence in fragments shorter than    1339 bp
  20% of sequence in fragments shorter than    1649 bp
  25% of sequence in fragments shorter than    1951 bp
  30% of sequence in fragments shorter than    2254 bp
  35% of sequence in fragments shorter than    2568 bp
  40% of sequence in fragments shorter than    2889 bp
  45% of sequence in fragments shorter than    3227 bp
  50% of sequence in fragments shorter than    3585 bp
  55% of sequence in fragments shorter than    3969 bp
  60% of sequence in fragments shorter than    4390 bp
  65% of sequence in fragments shorter than    4848 bp
  70% of sequence in fragments shorter than    5363 bp
  75% of sequence in fragments shorter than    5955 bp
  80% of sequence in fragments shorter than    6659 bp
  85% of sequence in fragments shorter than    7566 bp
  90% of sequence in fragments shorter than    8812 bp
  95% of sequence in fragments shorter than   10957 bp

# BsmI.FseI (GAATGC+GGCCGGCC) double digest, 1562 thousand fragments (1549350+13662 sites), 1907 bp average fragment length
   5% of sequence in fragments shorter than     682 bp
  10% of sequence in fragments shorter than    1154 bp
  15% of sequence in fragments shorter than    1552 bp
  20% of sequence in fragments shorter than    1912 bp
  25% of sequence in fragments shorter than    2260 bp
  30% of sequence in fragments shorter than    2594 bp
  35% of sequence in fragments shorter than    2932 bp
  40% of sequence in fragments shorter than    3283 bp
  45% of sequence in fragments shorter than    3655 bp
  50% of sequence in fragments shorter than    4044 bp
  55% of sequence in fragments shorter than    4461 bp
  60% of sequence in fragments shorter than    4912 bp
  65% of sequence in fragments shorter than    5413 bp
  70% of sequence in fragments shorter than    5970 bp
  75% of sequence in fragments shorter than    6618 bp
  80% of sequence in fragments shorter than    7381 bp
  85% of sequence in fragments shorter than    8355 bp
  90% of sequence in fragments shorter than    9693 bp
  95% of sequence in fragments shorter than   11910 bp

# AccI.ApaI (GTMKAC+GGGCCC) double digest, 1560 thousand fragments (1101063+460339 sites), 1909 bp average fragment length
   5% of sequence in fragments shorter than     664 bp
  10% of sequence in fragments shorter than    1021 bp
  15% of sequence in fragments shorter than    1327 bp
  20% of sequence in fragments shorter than    1612 bp
  25% of sequence in fragments shorter than    1894 bp
  30% of sequence in fragments shorter than    2176 bp
  35% of sequence in fragments shorter than    2465 bp
  40% of sequence in fragments shorter than    2757 bp
  45% of sequence in fragments shorter than    3068 bp
  50% of sequence in fragments shorter than    3401 bp
  55% of sequence in fragments shorter than    3748 bp
  60% of sequence in fragments shorter than    4135 bp
  65% of sequence in fragments shorter than    4563 bp
  70% of sequence in fragments shorter than    5043 bp
  75% of sequence in fragments shorter than    5592 bp
  80% of sequence in fragments shorter than    6190 bp
  85% of sequence in fragments shorter than    7037 bp
  90% of sequence in fragments shorter than    8244 bp
  95% of sequence in fragments shorter than   10452 bp

# BsaAI.EcoRI (YACGTR+GAATTC) double digest, 1560 thousand fragments (713801+847401 sites), 1909 bp average fragment length
   5% of sequence in fragments shorter than     675 bp
  10% of sequence in fragments shorter than    1022 bp
  15% of sequence in fragments shorter than    1331 bp
  20% of sequence in fragments shorter than    1614 bp
  25% of sequence in fragments shorter than    1898 bp
  30% of sequence in fragments shorter than    2169 bp
  35% of sequence in fragments shorter than    2437 bp
  40% of sequence in fragments shorter than    2729 bp
  45% of sequence in fragments shorter than    3028 bp
  50% of sequence in fragments shorter than    3348 bp
  55% of sequence in fragments shorter than    3683 bp
  60% of sequence in fragments shorter than    4057 bp
  65% of sequence in fragments shorter than    4464 bp
  70% of sequence in fragments shorter than    4926 bp
  75% of sequence in fragments shorter than    5464 bp
  80% of sequence in fragments shorter than    6101 bp
  85% of sequence in fragments shorter than    6914 bp
  90% of sequence in fragments shorter than    8021 bp
  95% of sequence in fragments shorter than    9910 bp

# FauI.XhoI (CCCGC+CTCGAG) double digest, 1560 thousand fragments (1439772+121308 sites), 1909 bp average fragment length
   5% of sequence in fragments shorter than     656 bp
  10% of sequence in fragments shorter than    1151 bp
  15% of sequence in fragments shorter than    1618 bp
  20% of sequence in fragments shorter than    2094 bp
  25% of sequence in fragments shorter than    2601 bp
  30% of sequence in fragments shorter than    3111 bp
  35% of sequence in fragments shorter than    3675 bp
  40% of sequence in fragments shorter than    4286 bp
  45% of sequence in fragments shorter than    4945 bp
  50% of sequence in fragments shorter than    5668 bp
  55% of sequence in fragments shorter than    6469 bp
  60% of sequence in fragments shorter than    7368 bp
  65% of sequence in fragments shorter than    8383 bp
  70% of sequence in fragments shorter than    9574 bp
  75% of sequence in fragments shorter than   11000 bp
  80% of sequence in fragments shorter than   12751 bp
  85% of sequence in fragments shorter than   15059 bp
  90% of sequence in fragments shorter than   18474 bp
  95% of sequence in fragments shorter than   24973 bp

# HgiCI.NotI (GGYRCC+GCGGCCGC) double digest, 1560 thousand fragments (1550877+9795 sites), 1909 bp average fragment length
   5% of sequence in fragments shorter than     627 bp
  10% of sequence in fragments shorter than     989 bp
  15% of sequence in fragments shorter than    1288 bp
  20% of sequence in fragments shorter than    1591 bp
  25% of sequence in fragments shorter than    1910 bp
  30% of sequence in fragments shorter than    2234 bp
  35% of sequence in fragments shorter than    2572 bp
  40% of sequence in fragments shorter than    2907 bp
  45% of sequence in fragments shorter than    3278 bp
  50% of sequence in fragments shorter than    3685 bp
  55% of sequence in fragments shorter than    4128 bp
  60% of sequence in fragments shorter than    4616 bp
  65% of sequence in fragments shorter than    5177 bp
  70% of sequence in fragments shorter than    5819 bp
  75% of sequence in fragments shorter than    6587 bp
  80% of sequence in fragments shorter than    7549 bp
  85% of sequence in fragments shorter than    8805 bp
  90% of sequence in fragments shorter than   10676 bp
  95% of sequence in fragments shorter than   14662 bp

# HgiCI.RsrII (GGYRCC+CGGWCCG) double digest, 1560 thousand fragments (1550877+9764 sites), 1909 bp average fragment length
   5% of sequence in fragments shorter than     627 bp
  10% of sequence in fragments shorter than     987 bp
  15% of sequence in fragments shorter than    1286 bp
  20% of sequence in fragments shorter than    1589 bp
  25% of sequence in fragments shorter than    1907 bp
  30% of sequence in fragments shorter than    2231 bp
  35% of sequence in fragments shorter than    2568 bp
  40% of sequence in fragments shorter than    2903 bp
  45% of sequence in fragments shorter than    3273 bp
  50% of sequence in fragments shorter than    3681 bp
  55% of sequence in fragments shorter than    4122 bp
  60% of sequence in fragments shorter than    4610 bp
  65% of sequence in fragments shorter than    5171 bp
  70% of sequence in fragments shorter than    5811 bp
  75% of sequence in fragments shorter than    6579 bp
  80% of sequence in fragments shorter than    7542 bp
  85% of sequence in fragments shorter than    8796 bp
  90% of sequence in fragments shorter than   10667 bp
  95% of sequence in fragments shorter than   14643 bp

# BspMI.XhoI (ACCTGC+CTCGAG) double digest, 1558 thousand fragments (1438129+121308 sites), 1911 bp average fragment length
   5% of sequence in fragments shorter than     644 bp
  10% of sequence in fragments shorter than     988 bp
  15% of sequence in fragments shorter than    1305 bp
  20% of sequence in fragments shorter than    1608 bp
  25% of sequence in fragments shorter than    1918 bp
  30% of sequence in fragments shorter than    2219 bp
  35% of sequence in fragments shorter than    2539 bp
  40% of sequence in fragments shorter than    2867 bp
  45% of sequence in fragments shorter than    3220 bp
  50% of sequence in fragments shorter than    3543 bp
  55% of sequence in fragments shorter than    3949 bp
  60% of sequence in fragments shorter than    4393 bp
  65% of sequence in fragments shorter than    4882 bp
  70% of sequence in fragments shorter than    5446 bp
  75% of sequence in fragments shorter than    6100 bp
  80% of sequence in fragments shorter than    6896 bp
  85% of sequence in fragments shorter than    7921 bp
  90% of sequence in fragments shorter than    9359 bp
  95% of sequence in fragments shorter than   11890 bp

# BsmI.NotI (GAATGC+GCGGCCGC) double digest, 1558 thousand fragments (1549350+9795 sites), 1911 bp average fragment length
   5% of sequence in fragments shorter than     684 bp
  10% of sequence in fragments shorter than    1157 bp
  15% of sequence in fragments shorter than    1557 bp
  20% of sequence in fragments shorter than    1918 bp
  25% of sequence in fragments shorter than    2267 bp
  30% of sequence in fragments shorter than    2603 bp
  35% of sequence in fragments shorter than    2941 bp
  40% of sequence in fragments shorter than    3294 bp
  45% of sequence in fragments shorter than    3667 bp
  50% of sequence in fragments shorter than    4059 bp
  55% of sequence in fragments shorter than    4477 bp
  60% of sequence in fragments shorter than    4932 bp
  65% of sequence in fragments shorter than    5435 bp
  70% of sequence in fragments shorter than    5993 bp
  75% of sequence in fragments shorter than    6645 bp
  80% of sequence in fragments shorter than    7408 bp
  85% of sequence in fragments shorter than    8392 bp
  90% of sequence in fragments shorter than    9733 bp
  95% of sequence in fragments shorter than   11968 bp

# BsmI.RsrII (GAATGC+CGGWCCG) double digest, 1558 thousand fragments (1549350+9764 sites), 1911 bp average fragment length
   5% of sequence in fragments shorter than     684 bp
  10% of sequence in fragments shorter than    1157 bp
  15% of sequence in fragments shorter than    1557 bp
  20% of sequence in fragments shorter than    1917 bp
  25% of sequence in fragments shorter than    2266 bp
  30% of sequence in fragments shorter than    2602 bp
  35% of sequence in fragments shorter than    2940 bp
  40% of sequence in fragments shorter than    3294 bp
  45% of sequence in fragments shorter than    3666 bp
  50% of sequence in fragments shorter than    4058 bp
  55% of sequence in fragments shorter than    4475 bp
  60% of sequence in fragments shorter than    4929 bp
  65% of sequence in fragments shorter than    5432 bp
  70% of sequence in fragments shorter than    5990 bp
  75% of sequence in fragments shorter than    6643 bp
  80% of sequence in fragments shorter than    7407 bp
  85% of sequence in fragments shorter than    8389 bp
  90% of sequence in fragments shorter than    9738 bp
  95% of sequence in fragments shorter than   11971 bp

# BsrBI.PstI (CCGCTC+CTGCAG) double digest, 1556 thousand fragments (235523+1321469 sites), 1914 bp average fragment length
   5% of sequence in fragments shorter than     630 bp
  10% of sequence in fragments shorter than     992 bp
  15% of sequence in fragments shorter than    1322 bp
  20% of sequence in fragments shorter than    1649 bp
  25% of sequence in fragments shorter than    1981 bp
  30% of sequence in fragments shorter than    2315 bp
  35% of sequence in fragments shorter than    2674 bp
  40% of sequence in fragments shorter than    3049 bp
  45% of sequence in fragments shorter than    3444 bp
  50% of sequence in fragments shorter than    3873 bp
  55% of sequence in fragments shorter than    4338 bp
  60% of sequence in fragments shorter than    4854 bp
  65% of sequence in fragments shorter than    5428 bp
  70% of sequence in fragments shorter than    6079 bp
  75% of sequence in fragments shorter than    6854 bp
  80% of sequence in fragments shorter than    7796 bp
  85% of sequence in fragments shorter than    9028 bp
  90% of sequence in fragments shorter than   10772 bp
  95% of sequence in fragments shorter than   13968 bp

# AvaI.SphI (CYCGRG+GCATGC) double digest, 1556 thousand fragments (1005714+550952 sites), 1914 bp average fragment length
   5% of sequence in fragments shorter than     639 bp
  10% of sequence in fragments shorter than    1018 bp
  15% of sequence in fragments shorter than    1364 bp
  20% of sequence in fragments shorter than    1705 bp
  25% of sequence in fragments shorter than    2049 bp
  30% of sequence in fragments shorter than    2409 bp
  35% of sequence in fragments shorter than    2788 bp
  40% of sequence in fragments shorter than    3193 bp
  45% of sequence in fragments shorter than    3632 bp
  50% of sequence in fragments shorter than    4108 bp
  55% of sequence in fragments shorter than    4633 bp
  60% of sequence in fragments shorter than    5206 bp
  65% of sequence in fragments shorter than    5859 bp
  70% of sequence in fragments shorter than    6609 bp
  75% of sequence in fragments shorter than    7497 bp
  80% of sequence in fragments shorter than    8605 bp
  85% of sequence in fragments shorter than   10072 bp
  90% of sequence in fragments shorter than   12227 bp
  95% of sequence in fragments shorter than   16518 bp

# AatII.AflIII (GACGTC+ACRYGT) double digest, 1556 thousand fragments (71089+1485394 sites), 1915 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1075 bp
  15% of sequence in fragments shorter than    1402 bp
  20% of sequence in fragments shorter than    1711 bp
  25% of sequence in fragments shorter than    2004 bp
  30% of sequence in fragments shorter than    2294 bp
  35% of sequence in fragments shorter than    2596 bp
  40% of sequence in fragments shorter than    2902 bp
  45% of sequence in fragments shorter than    3221 bp
  50% of sequence in fragments shorter than    3566 bp
  55% of sequence in fragments shorter than    3936 bp
  60% of sequence in fragments shorter than    4341 bp
  65% of sequence in fragments shorter than    4791 bp
  70% of sequence in fragments shorter than    5303 bp
  75% of sequence in fragments shorter than    5890 bp
  80% of sequence in fragments shorter than    6582 bp
  85% of sequence in fragments shorter than    7472 bp
  90% of sequence in fragments shorter than    8739 bp
  95% of sequence in fragments shorter than   11103 bp

# BciVI.HindIII (GTATCC+AAGCTT) double digest, 1556 thousand fragments (696093+860365 sites), 1915 bp average fragment length
   5% of sequence in fragments shorter than     662 bp
  10% of sequence in fragments shorter than    1010 bp
  15% of sequence in fragments shorter than    1304 bp
  20% of sequence in fragments shorter than    1587 bp
  25% of sequence in fragments shorter than    1859 bp
  30% of sequence in fragments shorter than    2112 bp
  35% of sequence in fragments shorter than    2386 bp
  40% of sequence in fragments shorter than    2665 bp
  45% of sequence in fragments shorter than    2964 bp
  50% of sequence in fragments shorter than    3281 bp
  55% of sequence in fragments shorter than    3625 bp
  60% of sequence in fragments shorter than    4002 bp
  65% of sequence in fragments shorter than    4414 bp
  70% of sequence in fragments shorter than    4889 bp
  75% of sequence in fragments shorter than    5444 bp
  80% of sequence in fragments shorter than    6110 bp
  85% of sequence in fragments shorter than    6992 bp
  90% of sequence in fragments shorter than    8248 bp
  95% of sequence in fragments shorter than   10569 bp

# BceAI.XbaI (ACGGC+TCTAGA) double digest, 1555 thousand fragments (705178+850998 sites), 1915 bp average fragment length
   5% of sequence in fragments shorter than     672 bp
  10% of sequence in fragments shorter than    1012 bp
  15% of sequence in fragments shorter than    1327 bp
  20% of sequence in fragments shorter than    1607 bp
  25% of sequence in fragments shorter than    1895 bp
  30% of sequence in fragments shorter than    2189 bp
  35% of sequence in fragments shorter than    2476 bp
  40% of sequence in fragments shorter than    2773 bp
  45% of sequence in fragments shorter than    3085 bp
  50% of sequence in fragments shorter than    3419 bp
  55% of sequence in fragments shorter than    3780 bp
  60% of sequence in fragments shorter than    4172 bp
  65% of sequence in fragments shorter than    4610 bp
  70% of sequence in fragments shorter than    5106 bp
  75% of sequence in fragments shorter than    5669 bp
  80% of sequence in fragments shorter than    6355 bp
  85% of sequence in fragments shorter than    7224 bp
  90% of sequence in fragments shorter than    8438 bp
  95% of sequence in fragments shorter than   10559 bp

# HaeII.StuI (RGCGCY+AGGCCT) double digest, 1555 thousand fragments (630101+925729 sites), 1915 bp average fragment length
   5% of sequence in fragments shorter than     585 bp
  10% of sequence in fragments shorter than     978 bp
  15% of sequence in fragments shorter than    1391 bp
  20% of sequence in fragments shorter than    1792 bp
  25% of sequence in fragments shorter than    2166 bp
  30% of sequence in fragments shorter than    2579 bp
  35% of sequence in fragments shorter than    2999 bp
  40% of sequence in fragments shorter than    3437 bp
  45% of sequence in fragments shorter than    3902 bp
  50% of sequence in fragments shorter than    4402 bp
  55% of sequence in fragments shorter than    4942 bp
  60% of sequence in fragments shorter than    5538 bp
  65% of sequence in fragments shorter than    6198 bp
  70% of sequence in fragments shorter than    6954 bp
  75% of sequence in fragments shorter than    7832 bp
  80% of sequence in fragments shorter than    8886 bp
  85% of sequence in fragments shorter than   10232 bp
  90% of sequence in fragments shorter than   12119 bp
  95% of sequence in fragments shorter than   15343 bp

# AscI.HgiCI (GGCGCGCC+GGYRCC) double digest, 1555 thousand fragments (4751+1550877 sites), 1916 bp average fragment length
   5% of sequence in fragments shorter than     629 bp
  10% of sequence in fragments shorter than     991 bp
  15% of sequence in fragments shorter than    1290 bp
  20% of sequence in fragments shorter than    1592 bp
  25% of sequence in fragments shorter than    1911 bp
  30% of sequence in fragments shorter than    2236 bp
  35% of sequence in fragments shorter than    2574 bp
  40% of sequence in fragments shorter than    2909 bp
  45% of sequence in fragments shorter than    3279 bp
  50% of sequence in fragments shorter than    3687 bp
  55% of sequence in fragments shorter than    4130 bp
  60% of sequence in fragments shorter than    4618 bp
  65% of sequence in fragments shorter than    5179 bp
  70% of sequence in fragments shorter than    5821 bp
  75% of sequence in fragments shorter than    6590 bp
  80% of sequence in fragments shorter than    7552 bp
  85% of sequence in fragments shorter than    8808 bp
  90% of sequence in fragments shorter than   10679 bp
  95% of sequence in fragments shorter than   14664 bp

# AscI.BsmI (GGCGCGCC+GAATGC) double digest, 1553 thousand fragments (4751+1549350 sites), 1918 bp average fragment length
   5% of sequence in fragments shorter than     687 bp
  10% of sequence in fragments shorter than    1162 bp
  15% of sequence in fragments shorter than    1563 bp
  20% of sequence in fragments shorter than    1925 bp
  25% of sequence in fragments shorter than    2276 bp
  30% of sequence in fragments shorter than    2613 bp
  35% of sequence in fragments shorter than    2953 bp
  40% of sequence in fragments shorter than    3307 bp
  45% of sequence in fragments shorter than    3681 bp
  50% of sequence in fragments shorter than    4074 bp
  55% of sequence in fragments shorter than    4494 bp
  60% of sequence in fragments shorter than    4950 bp
  65% of sequence in fragments shorter than    5456 bp
  70% of sequence in fragments shorter than    6019 bp
  75% of sequence in fragments shorter than    6673 bp
  80% of sequence in fragments shorter than    7442 bp
  85% of sequence in fragments shorter than    8428 bp
  90% of sequence in fragments shorter than    9786 bp
  95% of sequence in fragments shorter than   12044 bp

# BceAI.EcoRI (ACGGC+GAATTC) double digest, 1552 thousand fragments (705178+847401 sites), 1919 bp average fragment length
   5% of sequence in fragments shorter than     654 bp
  10% of sequence in fragments shorter than    1011 bp
  15% of sequence in fragments shorter than    1341 bp
  20% of sequence in fragments shorter than    1645 bp
  25% of sequence in fragments shorter than    1948 bp
  30% of sequence in fragments shorter than    2250 bp
  35% of sequence in fragments shorter than    2558 bp
  40% of sequence in fragments shorter than    2860 bp
  45% of sequence in fragments shorter than    3188 bp
  50% of sequence in fragments shorter than    3529 bp
  55% of sequence in fragments shorter than    3902 bp
  60% of sequence in fragments shorter than    4309 bp
  65% of sequence in fragments shorter than    4761 bp
  70% of sequence in fragments shorter than    5272 bp
  75% of sequence in fragments shorter than    5869 bp
  80% of sequence in fragments shorter than    6577 bp
  85% of sequence in fragments shorter than    7474 bp
  90% of sequence in fragments shorter than    8727 bp
  95% of sequence in fragments shorter than   10956 bp

# AflIII.SacII (ACRYGT+CCGCGG) double digest, 1551 thousand fragments (1485394+66901 sites), 1920 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1083 bp
  15% of sequence in fragments shorter than    1419 bp
  20% of sequence in fragments shorter than    1734 bp
  25% of sequence in fragments shorter than    2034 bp
  30% of sequence in fragments shorter than    2329 bp
  35% of sequence in fragments shorter than    2636 bp
  40% of sequence in fragments shorter than    2942 bp
  45% of sequence in fragments shorter than    3271 bp
  50% of sequence in fragments shorter than    3623 bp
  55% of sequence in fragments shorter than    4001 bp
  60% of sequence in fragments shorter than    4412 bp
  65% of sequence in fragments shorter than    4869 bp
  70% of sequence in fragments shorter than    5388 bp
  75% of sequence in fragments shorter than    5984 bp
  80% of sequence in fragments shorter than    6684 bp
  85% of sequence in fragments shorter than    7590 bp
  90% of sequence in fragments shorter than    8883 bp
  95% of sequence in fragments shorter than   11289 bp

# KasI.PstI (GGCGCC+CTGCAG) double digest, 1551 thousand fragments (230381+1321469 sites), 1920 bp average fragment length
   5% of sequence in fragments shorter than     634 bp
  10% of sequence in fragments shorter than     988 bp
  15% of sequence in fragments shorter than    1319 bp
  20% of sequence in fragments shorter than    1639 bp
  25% of sequence in fragments shorter than    1962 bp
  30% of sequence in fragments shorter than    2287 bp
  35% of sequence in fragments shorter than    2634 bp
  40% of sequence in fragments shorter than    2998 bp
  45% of sequence in fragments shorter than    3391 bp
  50% of sequence in fragments shorter than    3814 bp
  55% of sequence in fragments shorter than    4276 bp
  60% of sequence in fragments shorter than    4790 bp
  65% of sequence in fragments shorter than    5353 bp
  70% of sequence in fragments shorter than    5996 bp
  75% of sequence in fragments shorter than    6760 bp
  80% of sequence in fragments shorter than    7700 bp
  85% of sequence in fragments shorter than    8918 bp
  90% of sequence in fragments shorter than   10682 bp
  95% of sequence in fragments shorter than   14057 bp

# HgiCI.KpnI (GGYRCC+GGTACC) double digest, 1550 thousand fragments (1550877+287974 sites), 1921 bp average fragment length
   5% of sequence in fragments shorter than     631 bp
  10% of sequence in fragments shorter than     993 bp
  15% of sequence in fragments shorter than    1292 bp
  20% of sequence in fragments shorter than    1594 bp
  25% of sequence in fragments shorter than    1914 bp
  30% of sequence in fragments shorter than    2238 bp
  35% of sequence in fragments shorter than    2577 bp
  40% of sequence in fragments shorter than    2912 bp
  45% of sequence in fragments shorter than    3283 bp
  50% of sequence in fragments shorter than    3690 bp
  55% of sequence in fragments shorter than    4132 bp
  60% of sequence in fragments shorter than    4621 bp
  65% of sequence in fragments shorter than    5183 bp
  70% of sequence in fragments shorter than    5825 bp
  75% of sequence in fragments shorter than    6593 bp
  80% of sequence in fragments shorter than    7556 bp
  85% of sequence in fragments shorter than    8811 bp
  90% of sequence in fragments shorter than   10683 bp
  95% of sequence in fragments shorter than   14669 bp

# HgiCI.KasI (GGYRCC+GGCGCC) double digest, 1550 thousand fragments (1550877+230381 sites), 1921 bp average fragment length
   5% of sequence in fragments shorter than     631 bp
  10% of sequence in fragments shorter than     993 bp
  15% of sequence in fragments shorter than    1292 bp
  20% of sequence in fragments shorter than    1594 bp
  25% of sequence in fragments shorter than    1914 bp
  30% of sequence in fragments shorter than    2238 bp
  35% of sequence in fragments shorter than    2577 bp
  40% of sequence in fragments shorter than    2912 bp
  45% of sequence in fragments shorter than    3283 bp
  50% of sequence in fragments shorter than    3690 bp
  55% of sequence in fragments shorter than    4132 bp
  60% of sequence in fragments shorter than    4621 bp
  65% of sequence in fragments shorter than    5183 bp
  70% of sequence in fragments shorter than    5825 bp
  75% of sequence in fragments shorter than    6593 bp
  80% of sequence in fragments shorter than    7556 bp
  85% of sequence in fragments shorter than    8811 bp
  90% of sequence in fragments shorter than   10683 bp
  95% of sequence in fragments shorter than   14669 bp

# BspHI.HgaI (TCATGA+GACGC) double digest, 1549 thousand fragments (978290+571889 sites), 1922 bp average fragment length
   5% of sequence in fragments shorter than     680 bp
  10% of sequence in fragments shorter than    1040 bp
  15% of sequence in fragments shorter than    1356 bp
  20% of sequence in fragments shorter than    1651 bp
  25% of sequence in fragments shorter than    1937 bp
  30% of sequence in fragments shorter than    2212 bp
  35% of sequence in fragments shorter than    2500 bp
  40% of sequence in fragments shorter than    2795 bp
  45% of sequence in fragments shorter than    3101 bp
  50% of sequence in fragments shorter than    3430 bp
  55% of sequence in fragments shorter than    3787 bp
  60% of sequence in fragments shorter than    4164 bp
  65% of sequence in fragments shorter than    4584 bp
  70% of sequence in fragments shorter than    5069 bp
  75% of sequence in fragments shorter than    5634 bp
  80% of sequence in fragments shorter than    6305 bp
  85% of sequence in fragments shorter than    7169 bp
  90% of sequence in fragments shorter than    8414 bp
  95% of sequence in fragments shorter than   10745 bp

# BspHI.EciI (TCATGA+GGCGGA) double digest, 1549 thousand fragments (978290+571273 sites), 1923 bp average fragment length
   5% of sequence in fragments shorter than     677 bp
  10% of sequence in fragments shorter than    1038 bp
  15% of sequence in fragments shorter than    1353 bp
  20% of sequence in fragments shorter than    1653 bp
  25% of sequence in fragments shorter than    1939 bp
  30% of sequence in fragments shorter than    2213 bp
  35% of sequence in fragments shorter than    2502 bp
  40% of sequence in fragments shorter than    2803 bp
  45% of sequence in fragments shorter than    3114 bp
  50% of sequence in fragments shorter than    3452 bp
  55% of sequence in fragments shorter than    3810 bp
  60% of sequence in fragments shorter than    4196 bp
  65% of sequence in fragments shorter than    4629 bp
  70% of sequence in fragments shorter than    5121 bp
  75% of sequence in fragments shorter than    5689 bp
  80% of sequence in fragments shorter than    6391 bp
  85% of sequence in fragments shorter than    7287 bp
  90% of sequence in fragments shorter than    8589 bp
  95% of sequence in fragments shorter than   11146 bp

# AvaI.ScaI (CYCGRG+AGTACT) double digest, 1549 thousand fragments (1005714+543793 sites), 1923 bp average fragment length
   5% of sequence in fragments shorter than     650 bp
  10% of sequence in fragments shorter than    1023 bp
  15% of sequence in fragments shorter than    1355 bp
  20% of sequence in fragments shorter than    1674 bp
  25% of sequence in fragments shorter than    1988 bp
  30% of sequence in fragments shorter than    2314 bp
  35% of sequence in fragments shorter than    2647 bp
  40% of sequence in fragments shorter than    2999 bp
  45% of sequence in fragments shorter than    3375 bp
  50% of sequence in fragments shorter than    3779 bp
  55% of sequence in fragments shorter than    4220 bp
  60% of sequence in fragments shorter than    4700 bp
  65% of sequence in fragments shorter than    5234 bp
  70% of sequence in fragments shorter than    5845 bp
  75% of sequence in fragments shorter than    6573 bp
  80% of sequence in fragments shorter than    7480 bp
  85% of sequence in fragments shorter than    8652 bp
  90% of sequence in fragments shorter than   10384 bp
  95% of sequence in fragments shorter than   13895 bp

# PstI.SwaI (CTGCAG+ATTTAAAT) double digest, 1547 thousand fragments (1321469+226602 sites), 1925 bp average fragment length
   5% of sequence in fragments shorter than     657 bp
  10% of sequence in fragments shorter than    1005 bp
  15% of sequence in fragments shorter than    1321 bp
  20% of sequence in fragments shorter than    1618 bp
  25% of sequence in fragments shorter than    1916 bp
  30% of sequence in fragments shorter than    2211 bp
  35% of sequence in fragments shorter than    2521 bp
  40% of sequence in fragments shorter than    2841 bp
  45% of sequence in fragments shorter than    3181 bp
  50% of sequence in fragments shorter than    3543 bp
  55% of sequence in fragments shorter than    3937 bp
  60% of sequence in fragments shorter than    4367 bp
  65% of sequence in fragments shorter than    4845 bp
  70% of sequence in fragments shorter than    5379 bp
  75% of sequence in fragments shorter than    5993 bp
  80% of sequence in fragments shorter than    6750 bp
  85% of sequence in fragments shorter than    7722 bp
  90% of sequence in fragments shorter than    9099 bp
  95% of sequence in fragments shorter than   11649 bp

# AccI.EcoRV (GTMKAC+GATATC) double digest, 1547 thousand fragments (1101063+446473 sites), 1926 bp average fragment length
   5% of sequence in fragments shorter than     671 bp
  10% of sequence in fragments shorter than    1022 bp
  15% of sequence in fragments shorter than    1324 bp
  20% of sequence in fragments shorter than    1609 bp
  25% of sequence in fragments shorter than    1886 bp
  30% of sequence in fragments shorter than    2159 bp
  35% of sequence in fragments shorter than    2438 bp
  40% of sequence in fragments shorter than    2727 bp
  45% of sequence in fragments shorter than    3004 bp
  50% of sequence in fragments shorter than    3322 bp
  55% of sequence in fragments shorter than    3673 bp
  60% of sequence in fragments shorter than    4064 bp
  65% of sequence in fragments shorter than    4496 bp
  70% of sequence in fragments shorter than    4989 bp
  75% of sequence in fragments shorter than    5565 bp
  80% of sequence in fragments shorter than    6274 bp
  85% of sequence in fragments shorter than    7207 bp
  90% of sequence in fragments shorter than    8565 bp
  95% of sequence in fragments shorter than   11082 bp

# BciVI.XbaI (GTATCC+TCTAGA) double digest, 1546 thousand fragments (696093+850998 sites), 1926 bp average fragment length
   5% of sequence in fragments shorter than     687 bp
  10% of sequence in fragments shorter than    1006 bp
  15% of sequence in fragments shorter than    1301 bp
  20% of sequence in fragments shorter than    1581 bp
  25% of sequence in fragments shorter than    1863 bp
  30% of sequence in fragments shorter than    2142 bp
  35% of sequence in fragments shorter than    2434 bp
  40% of sequence in fragments shorter than    2736 bp
  45% of sequence in fragments shorter than    3046 bp
  50% of sequence in fragments shorter than    3378 bp
  55% of sequence in fragments shorter than    3730 bp
  60% of sequence in fragments shorter than    4114 bp
  65% of sequence in fragments shorter than    4541 bp
  70% of sequence in fragments shorter than    5025 bp
  75% of sequence in fragments shorter than    5596 bp
  80% of sequence in fragments shorter than    6283 bp
  85% of sequence in fragments shorter than    7177 bp
  90% of sequence in fragments shorter than    8457 bp
  95% of sequence in fragments shorter than   10755 bp

# AflII.NdeI (CTTAAG+CATATG) double digest, 1546 thousand fragments (643284+903360 sites), 1927 bp average fragment length
   5% of sequence in fragments shorter than     650 bp
  10% of sequence in fragments shorter than    1000 bp
  15% of sequence in fragments shorter than    1291 bp
  20% of sequence in fragments shorter than    1583 bp
  25% of sequence in fragments shorter than    1870 bp
  30% of sequence in fragments shorter than    2158 bp
  35% of sequence in fragments shorter than    2455 bp
  40% of sequence in fragments shorter than    2765 bp
  45% of sequence in fragments shorter than    3093 bp
  50% of sequence in fragments shorter than    3443 bp
  55% of sequence in fragments shorter than    3817 bp
  60% of sequence in fragments shorter than    4236 bp
  65% of sequence in fragments shorter than    4691 bp
  70% of sequence in fragments shorter than    5213 bp
  75% of sequence in fragments shorter than    5856 bp
  80% of sequence in fragments shorter than    6639 bp
  85% of sequence in fragments shorter than    7728 bp
  90% of sequence in fragments shorter than    9425 bp
  95% of sequence in fragments shorter than   13266 bp

# ApaI.PpuMI (GGGCCC+RGGWCCY) double digest, 1545 thousand fragments (460339+1085138 sites), 1928 bp average fragment length
   5% of sequence in fragments shorter than     598 bp
  10% of sequence in fragments shorter than    1010 bp
  15% of sequence in fragments shorter than    1407 bp
  20% of sequence in fragments shorter than    1801 bp
  25% of sequence in fragments shorter than    2191 bp
  30% of sequence in fragments shorter than    2600 bp
  35% of sequence in fragments shorter than    3029 bp
  40% of sequence in fragments shorter than    3468 bp
  45% of sequence in fragments shorter than    3894 bp
  50% of sequence in fragments shorter than    4409 bp
  55% of sequence in fragments shorter than    4976 bp
  60% of sequence in fragments shorter than    5603 bp
  65% of sequence in fragments shorter than    6316 bp
  70% of sequence in fragments shorter than    7130 bp
  75% of sequence in fragments shorter than    8115 bp
  80% of sequence in fragments shorter than    9308 bp
  85% of sequence in fragments shorter than   10887 bp
  90% of sequence in fragments shorter than   13288 bp
  95% of sequence in fragments shorter than   18270 bp

# ApaI.PvuII (GGGCCC+CAGCTG) double digest, 1544 thousand fragments (460339+1084260 sites), 1929 bp average fragment length
   5% of sequence in fragments shorter than     617 bp
  10% of sequence in fragments shorter than    1007 bp
  15% of sequence in fragments shorter than    1370 bp
  20% of sequence in fragments shorter than    1723 bp
  25% of sequence in fragments shorter than    2084 bp
  30% of sequence in fragments shorter than    2458 bp
  35% of sequence in fragments shorter than    2836 bp
  40% of sequence in fragments shorter than    3244 bp
  45% of sequence in fragments shorter than    3679 bp
  50% of sequence in fragments shorter than    4159 bp
  55% of sequence in fragments shorter than    4680 bp
  60% of sequence in fragments shorter than    5257 bp
  65% of sequence in fragments shorter than    5892 bp
  70% of sequence in fragments shorter than    6624 bp
  75% of sequence in fragments shorter than    7475 bp
  80% of sequence in fragments shorter than    8536 bp
  85% of sequence in fragments shorter than    9941 bp
  90% of sequence in fragments shorter than   12015 bp
  95% of sequence in fragments shorter than   16156 bp

# AflIII.PspXI (ACRYGT+VCTCGAGB) double digest, 1544 thousand fragments (1485394+59193 sites), 1929 bp average fragment length
   5% of sequence in fragments shorter than     696 bp
  10% of sequence in fragments shorter than    1084 bp
  15% of sequence in fragments shorter than    1414 bp
  20% of sequence in fragments shorter than    1725 bp
  25% of sequence in fragments shorter than    2020 bp
  30% of sequence in fragments shorter than    2311 bp
  35% of sequence in fragments shorter than    2614 bp
  40% of sequence in fragments shorter than    2922 bp
  45% of sequence in fragments shorter than    3241 bp
  50% of sequence in fragments shorter than    3587 bp
  55% of sequence in fragments shorter than    3959 bp
  60% of sequence in fragments shorter than    4365 bp
  65% of sequence in fragments shorter than    4818 bp
  70% of sequence in fragments shorter than    5328 bp
  75% of sequence in fragments shorter than    5912 bp
  80% of sequence in fragments shorter than    6606 bp
  85% of sequence in fragments shorter than    7501 bp
  90% of sequence in fragments shorter than    8779 bp
  95% of sequence in fragments shorter than   11152 bp

# BciVI.EcoRI (GTATCC+GAATTC) double digest, 1543 thousand fragments (696093+847401 sites), 1931 bp average fragment length
   5% of sequence in fragments shorter than     673 bp
  10% of sequence in fragments shorter than    1001 bp
  15% of sequence in fragments shorter than    1324 bp
  20% of sequence in fragments shorter than    1618 bp
  25% of sequence in fragments shorter than    1900 bp
  30% of sequence in fragments shorter than    2177 bp
  35% of sequence in fragments shorter than    2463 bp
  40% of sequence in fragments shorter than    2756 bp
  45% of sequence in fragments shorter than    3065 bp
  50% of sequence in fragments shorter than    3388 bp
  55% of sequence in fragments shorter than    3744 bp
  60% of sequence in fragments shorter than    4125 bp
  65% of sequence in fragments shorter than    4544 bp
  70% of sequence in fragments shorter than    5020 bp
  75% of sequence in fragments shorter than    5581 bp
  80% of sequence in fragments shorter than    6257 bp
  85% of sequence in fragments shorter than    7122 bp
  90% of sequence in fragments shorter than    8349 bp
  95% of sequence in fragments shorter than   10513 bp

# BspHI.MfeI (TCATGA+CAATTG) double digest, 1542 thousand fragments (978290+564303 sites), 1932 bp average fragment length
   5% of sequence in fragments shorter than     681 bp
  10% of sequence in fragments shorter than    1028 bp
  15% of sequence in fragments shorter than    1336 bp
  20% of sequence in fragments shorter than    1629 bp
  25% of sequence in fragments shorter than    1907 bp
  30% of sequence in fragments shorter than    2172 bp
  35% of sequence in fragments shorter than    2455 bp
  40% of sequence in fragments shorter than    2754 bp
  45% of sequence in fragments shorter than    3065 bp
  50% of sequence in fragments shorter than    3396 bp
  55% of sequence in fragments shorter than    3759 bp
  60% of sequence in fragments shorter than    4158 bp
  65% of sequence in fragments shorter than    4593 bp
  70% of sequence in fragments shorter than    5093 bp
  75% of sequence in fragments shorter than    5682 bp
  80% of sequence in fragments shorter than    6410 bp
  85% of sequence in fragments shorter than    7356 bp
  90% of sequence in fragments shorter than    8767 bp
  95% of sequence in fragments shorter than   11598 bp

# AflIII.AgeI (ACRYGT+ACCGGT) double digest, 1538 thousand fragments (1485394+53386 sites), 1937 bp average fragment length
   5% of sequence in fragments shorter than     699 bp
  10% of sequence in fragments shorter than    1090 bp
  15% of sequence in fragments shorter than    1423 bp
  20% of sequence in fragments shorter than    1737 bp
  25% of sequence in fragments shorter than    2034 bp
  30% of sequence in fragments shorter than    2326 bp
  35% of sequence in fragments shorter than    2630 bp
  40% of sequence in fragments shorter than    2935 bp
  45% of sequence in fragments shorter than    3257 bp
  50% of sequence in fragments shorter than    3603 bp
  55% of sequence in fragments shorter than    3957 bp
  60% of sequence in fragments shorter than    4359 bp
  65% of sequence in fragments shorter than    4811 bp
  70% of sequence in fragments shorter than    5323 bp
  75% of sequence in fragments shorter than    5915 bp
  80% of sequence in fragments shorter than    6620 bp
  85% of sequence in fragments shorter than    7536 bp
  90% of sequence in fragments shorter than    8831 bp
  95% of sequence in fragments shorter than   11264 bp

# AvaIII.SacI (ATGCAT+GAGCTC) double digest, 1536 thousand fragments (933357+603323 sites), 1939 bp average fragment length
   5% of sequence in fragments shorter than     686 bp
  10% of sequence in fragments shorter than    1039 bp
  15% of sequence in fragments shorter than    1343 bp
  20% of sequence in fragments shorter than    1627 bp
  25% of sequence in fragments shorter than    1907 bp
  30% of sequence in fragments shorter than    2181 bp
  35% of sequence in fragments shorter than    2463 bp
  40% of sequence in fragments shorter than    2753 bp
  45% of sequence in fragments shorter than    3056 bp
  50% of sequence in fragments shorter than    3382 bp
  55% of sequence in fragments shorter than    3733 bp
  60% of sequence in fragments shorter than    4114 bp
  65% of sequence in fragments shorter than    4530 bp
  70% of sequence in fragments shorter than    4998 bp
  75% of sequence in fragments shorter than    5553 bp
  80% of sequence in fragments shorter than    6216 bp
  85% of sequence in fragments shorter than    7074 bp
  90% of sequence in fragments shorter than    8303 bp
  95% of sequence in fragments shorter than   10662 bp

# BglII.NcoI (AGATCT+CCATGG) double digest, 1533 thousand fragments (774732+759594 sites), 1942 bp average fragment length
   5% of sequence in fragments shorter than     673 bp
  10% of sequence in fragments shorter than    1016 bp
  15% of sequence in fragments shorter than    1324 bp
  20% of sequence in fragments shorter than    1597 bp
  25% of sequence in fragments shorter than    1873 bp
  30% of sequence in fragments shorter than    2152 bp
  35% of sequence in fragments shorter than    2437 bp
  40% of sequence in fragments shorter than    2730 bp
  45% of sequence in fragments shorter than    3017 bp
  50% of sequence in fragments shorter than    3343 bp
  55% of sequence in fragments shorter than    3667 bp
  60% of sequence in fragments shorter than    4048 bp
  65% of sequence in fragments shorter than    4463 bp
  70% of sequence in fragments shorter than    4943 bp
  75% of sequence in fragments shorter than    5494 bp
  80% of sequence in fragments shorter than    6176 bp
  85% of sequence in fragments shorter than    7071 bp
  90% of sequence in fragments shorter than    8363 bp
  95% of sequence in fragments shorter than   10908 bp

# HaeII.NdeI (RGCGCY+CATATG) double digest, 1532 thousand fragments (630101+903360 sites), 1943 bp average fragment length
   5% of sequence in fragments shorter than     669 bp
  10% of sequence in fragments shorter than    1020 bp
  15% of sequence in fragments shorter than    1339 bp
  20% of sequence in fragments shorter than    1658 bp
  25% of sequence in fragments shorter than    1975 bp
  30% of sequence in fragments shorter than    2283 bp
  35% of sequence in fragments shorter than    2608 bp
  40% of sequence in fragments shorter than    2928 bp
  45% of sequence in fragments shorter than    3269 bp
  50% of sequence in fragments shorter than    3622 bp
  55% of sequence in fragments shorter than    4000 bp
  60% of sequence in fragments shorter than    4407 bp
  65% of sequence in fragments shorter than    4857 bp
  70% of sequence in fragments shorter than    5362 bp
  75% of sequence in fragments shorter than    5946 bp
  80% of sequence in fragments shorter than    6644 bp
  85% of sequence in fragments shorter than    7513 bp
  90% of sequence in fragments shorter than    8735 bp
  95% of sequence in fragments shorter than   10807 bp

# EcoRV.PpuMI (GATATC+RGGWCCY) double digest, 1531 thousand fragments (446473+1085138 sites), 1946 bp average fragment length
   5% of sequence in fragments shorter than     662 bp
  10% of sequence in fragments shorter than    1025 bp
  15% of sequence in fragments shorter than    1334 bp
  20% of sequence in fragments shorter than    1652 bp
  25% of sequence in fragments shorter than    1953 bp
  30% of sequence in fragments shorter than    2268 bp
  35% of sequence in fragments shorter than    2575 bp
  40% of sequence in fragments shorter than    2904 bp
  45% of sequence in fragments shorter than    3256 bp
  50% of sequence in fragments shorter than    3631 bp
  55% of sequence in fragments shorter than    4034 bp
  60% of sequence in fragments shorter than    4476 bp
  65% of sequence in fragments shorter than    4969 bp
  70% of sequence in fragments shorter than    5530 bp
  75% of sequence in fragments shorter than    6187 bp
  80% of sequence in fragments shorter than    6991 bp
  85% of sequence in fragments shorter than    8021 bp
  90% of sequence in fragments shorter than    9516 bp
  95% of sequence in fragments shorter than   12341 bp

# EcoRV.PvuII (GATATC+CAGCTG) double digest, 1530 thousand fragments (446473+1084260 sites), 1947 bp average fragment length
   5% of sequence in fragments shorter than     679 bp
  10% of sequence in fragments shorter than    1031 bp
  15% of sequence in fragments shorter than    1345 bp
  20% of sequence in fragments shorter than    1641 bp
  25% of sequence in fragments shorter than    1926 bp
  30% of sequence in fragments shorter than    2219 bp
  35% of sequence in fragments shorter than    2506 bp
  40% of sequence in fragments shorter than    2809 bp
  45% of sequence in fragments shorter than    3131 bp
  50% of sequence in fragments shorter than    3472 bp
  55% of sequence in fragments shorter than    3840 bp
  60% of sequence in fragments shorter than    4241 bp
  65% of sequence in fragments shorter than    4685 bp
  70% of sequence in fragments shorter than    5192 bp
  75% of sequence in fragments shorter than    5781 bp
  80% of sequence in fragments shorter than    6492 bp
  85% of sequence in fragments shorter than    7408 bp
  90% of sequence in fragments shorter than    8712 bp
  95% of sequence in fragments shorter than   11093 bp

# BspHI.SphI (TCATGA+GCATGC) double digest, 1528 thousand fragments (978290+550952 sites), 1949 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1037 bp
  15% of sequence in fragments shorter than    1339 bp
  20% of sequence in fragments shorter than    1623 bp
  25% of sequence in fragments shorter than    1897 bp
  30% of sequence in fragments shorter than    2156 bp
  35% of sequence in fragments shorter than    2429 bp
  40% of sequence in fragments shorter than    2714 bp
  45% of sequence in fragments shorter than    3009 bp
  50% of sequence in fragments shorter than    3320 bp
  55% of sequence in fragments shorter than    3655 bp
  60% of sequence in fragments shorter than    4022 bp
  65% of sequence in fragments shorter than    4426 bp
  70% of sequence in fragments shorter than    4883 bp
  75% of sequence in fragments shorter than    5414 bp
  80% of sequence in fragments shorter than    6058 bp
  85% of sequence in fragments shorter than    6898 bp
  90% of sequence in fragments shorter than    8096 bp
  95% of sequence in fragments shorter than   10414 bp

# SacI.StuI (GAGCTC+AGGCCT) double digest, 1528 thousand fragments (603323+925729 sites), 1949 bp average fragment length
   5% of sequence in fragments shorter than     631 bp
  10% of sequence in fragments shorter than     985 bp
  15% of sequence in fragments shorter than    1332 bp
  20% of sequence in fragments shorter than    1657 bp
  25% of sequence in fragments shorter than    1980 bp
  30% of sequence in fragments shorter than    2327 bp
  35% of sequence in fragments shorter than    2685 bp
  40% of sequence in fragments shorter than    3056 bp
  45% of sequence in fragments shorter than    3447 bp
  50% of sequence in fragments shorter than    3863 bp
  55% of sequence in fragments shorter than    4315 bp
  60% of sequence in fragments shorter than    4803 bp
  65% of sequence in fragments shorter than    5353 bp
  70% of sequence in fragments shorter than    5971 bp
  75% of sequence in fragments shorter than    6708 bp
  80% of sequence in fragments shorter than    7585 bp
  85% of sequence in fragments shorter than    8681 bp
  90% of sequence in fragments shorter than   10257 bp
  95% of sequence in fragments shorter than   12927 bp

# BscGI.SexAI (CCCGT+ACCWGGT) double digest, 1528 thousand fragments (1231070+297900 sites), 1949 bp average fragment length
   5% of sequence in fragments shorter than     572 bp
  10% of sequence in fragments shorter than     973 bp
  15% of sequence in fragments shorter than    1351 bp
  20% of sequence in fragments shorter than    1714 bp
  25% of sequence in fragments shorter than    2082 bp
  30% of sequence in fragments shorter than    2463 bp
  35% of sequence in fragments shorter than    2857 bp
  40% of sequence in fragments shorter than    3277 bp
  45% of sequence in fragments shorter than    3718 bp
  50% of sequence in fragments shorter than    4196 bp
  55% of sequence in fragments shorter than    4711 bp
  60% of sequence in fragments shorter than    5289 bp
  65% of sequence in fragments shorter than    5931 bp
  70% of sequence in fragments shorter than    6659 bp
  75% of sequence in fragments shorter than    7514 bp
  80% of sequence in fragments shorter than    8569 bp
  85% of sequence in fragments shorter than    9928 bp
  90% of sequence in fragments shorter than   11841 bp
  95% of sequence in fragments shorter than   15192 bp

# AvaIII.AvrII (ATGCAT+CCTAGG) double digest, 1527 thousand fragments (933357+594956 sites), 1950 bp average fragment length
   5% of sequence in fragments shorter than     679 bp
  10% of sequence in fragments shorter than    1045 bp
  15% of sequence in fragments shorter than    1354 bp
  20% of sequence in fragments shorter than    1641 bp
  25% of sequence in fragments shorter than    1922 bp
  30% of sequence in fragments shorter than    2196 bp
  35% of sequence in fragments shorter than    2476 bp
  40% of sequence in fragments shorter than    2766 bp
  45% of sequence in fragments shorter than    3074 bp
  50% of sequence in fragments shorter than    3392 bp
  55% of sequence in fragments shorter than    3739 bp
  60% of sequence in fragments shorter than    4120 bp
  65% of sequence in fragments shorter than    4536 bp
  70% of sequence in fragments shorter than    4999 bp
  75% of sequence in fragments shorter than    5543 bp
  80% of sequence in fragments shorter than    6200 bp
  85% of sequence in fragments shorter than    7057 bp
  90% of sequence in fragments shorter than    8285 bp
  95% of sequence in fragments shorter than   10668 bp

# AflIII.PmeI (ACRYGT+GTTTAAAC) double digest, 1527 thousand fragments (1485394+42094 sites), 1951 bp average fragment length
   5% of sequence in fragments shorter than     703 bp
  10% of sequence in fragments shorter than    1096 bp
  15% of sequence in fragments shorter than    1432 bp
  20% of sequence in fragments shorter than    1748 bp
  25% of sequence in fragments shorter than    2049 bp
  30% of sequence in fragments shorter than    2345 bp
  35% of sequence in fragments shorter than    2654 bp
  40% of sequence in fragments shorter than    2961 bp
  45% of sequence in fragments shorter than    3292 bp
  50% of sequence in fragments shorter than    3646 bp
  55% of sequence in fragments shorter than    4027 bp
  60% of sequence in fragments shorter than    4443 bp
  65% of sequence in fragments shorter than    4903 bp
  70% of sequence in fragments shorter than    5433 bp
  75% of sequence in fragments shorter than    6035 bp
  80% of sequence in fragments shorter than    6753 bp
  85% of sequence in fragments shorter than    7671 bp
  90% of sequence in fragments shorter than    8993 bp
  95% of sequence in fragments shorter than   11463 bp

# ClaI.FauI (ATCGAT+CCCGC) double digest, 1525 thousand fragments (86477+1439772 sites), 1953 bp average fragment length
   5% of sequence in fragments shorter than     681 bp
  10% of sequence in fragments shorter than    1190 bp
  15% of sequence in fragments shorter than    1664 bp
  20% of sequence in fragments shorter than    2136 bp
  25% of sequence in fragments shorter than    2635 bp
  30% of sequence in fragments shorter than    3156 bp
  35% of sequence in fragments shorter than    3703 bp
  40% of sequence in fragments shorter than    4269 bp
  45% of sequence in fragments shorter than    4901 bp
  50% of sequence in fragments shorter than    5600 bp
  55% of sequence in fragments shorter than    6376 bp
  60% of sequence in fragments shorter than    7233 bp
  65% of sequence in fragments shorter than    8213 bp
  70% of sequence in fragments shorter than    9326 bp
  75% of sequence in fragments shorter than   10658 bp
  80% of sequence in fragments shorter than   12290 bp
  85% of sequence in fragments shorter than   14459 bp
  90% of sequence in fragments shorter than   17619 bp
  95% of sequence in fragments shorter than   23696 bp

# BspMI.ClaI (ACCTGC+ATCGAT) double digest, 1524 thousand fragments (1438129+86477 sites), 1955 bp average fragment length
   5% of sequence in fragments shorter than     666 bp
  10% of sequence in fragments shorter than    1017 bp
  15% of sequence in fragments shorter than    1347 bp
  20% of sequence in fragments shorter than    1652 bp
  25% of sequence in fragments shorter than    1963 bp
  30% of sequence in fragments shorter than    2248 bp
  35% of sequence in fragments shorter than    2558 bp
  40% of sequence in fragments shorter than    2880 bp
  45% of sequence in fragments shorter than    3226 bp
  50% of sequence in fragments shorter than    3559 bp
  55% of sequence in fragments shorter than    3959 bp
  60% of sequence in fragments shorter than    4396 bp
  65% of sequence in fragments shorter than    4880 bp
  70% of sequence in fragments shorter than    5432 bp
  75% of sequence in fragments shorter than    6076 bp
  80% of sequence in fragments shorter than    6862 bp
  85% of sequence in fragments shorter than    7863 bp
  90% of sequence in fragments shorter than    9261 bp
  95% of sequence in fragments shorter than   11714 bp

# BspHI.ScaI (TCATGA+AGTACT) double digest, 1521 thousand fragments (978290+543793 sites), 1958 bp average fragment length
   5% of sequence in fragments shorter than     688 bp
  10% of sequence in fragments shorter than    1035 bp
  15% of sequence in fragments shorter than    1342 bp
  20% of sequence in fragments shorter than    1630 bp
  25% of sequence in fragments shorter than    1907 bp
  30% of sequence in fragments shorter than    2175 bp
  35% of sequence in fragments shorter than    2453 bp
  40% of sequence in fragments shorter than    2749 bp
  45% of sequence in fragments shorter than    3056 bp
  50% of sequence in fragments shorter than    3386 bp
  55% of sequence in fragments shorter than    3741 bp
  60% of sequence in fragments shorter than    4134 bp
  65% of sequence in fragments shorter than    4563 bp
  70% of sequence in fragments shorter than    5050 bp
  75% of sequence in fragments shorter than    5630 bp
  80% of sequence in fragments shorter than    6345 bp
  85% of sequence in fragments shorter than    7302 bp
  90% of sequence in fragments shorter than    8717 bp
  95% of sequence in fragments shorter than   11677 bp

# AvrII.StuI (CCTAGG+AGGCCT) double digest, 1520 thousand fragments (594956+925729 sites), 1960 bp average fragment length
   5% of sequence in fragments shorter than     645 bp
  10% of sequence in fragments shorter than    1004 bp
  15% of sequence in fragments shorter than    1360 bp
  20% of sequence in fragments shorter than    1688 bp
  25% of sequence in fragments shorter than    2010 bp
  30% of sequence in fragments shorter than    2355 bp
  35% of sequence in fragments shorter than    2695 bp
  40% of sequence in fragments shorter than    3058 bp
  45% of sequence in fragments shorter than    3435 bp
  50% of sequence in fragments shorter than    3831 bp
  55% of sequence in fragments shorter than    4260 bp
  60% of sequence in fragments shorter than    4728 bp
  65% of sequence in fragments shorter than    5250 bp
  70% of sequence in fragments shorter than    5847 bp
  75% of sequence in fragments shorter than    6542 bp
  80% of sequence in fragments shorter than    7385 bp
  85% of sequence in fragments shorter than    8430 bp
  90% of sequence in fragments shorter than    9927 bp
  95% of sequence in fragments shorter than   12465 bp

# BscGI.KpnI (CCCGT+GGTACC) double digest, 1518 thousand fragments (1231070+287974 sites), 1962 bp average fragment length
   5% of sequence in fragments shorter than     584 bp
  10% of sequence in fragments shorter than     993 bp
  15% of sequence in fragments shorter than    1353 bp
  20% of sequence in fragments shorter than    1706 bp
  25% of sequence in fragments shorter than    2073 bp
  30% of sequence in fragments shorter than    2448 bp
  35% of sequence in fragments shorter than    2833 bp
  40% of sequence in fragments shorter than    3244 bp
  45% of sequence in fragments shorter than    3674 bp
  50% of sequence in fragments shorter than    4138 bp
  55% of sequence in fragments shorter than    4638 bp
  60% of sequence in fragments shorter than    5206 bp
  65% of sequence in fragments shorter than    5838 bp
  70% of sequence in fragments shorter than    6554 bp
  75% of sequence in fragments shorter than    7384 bp
  80% of sequence in fragments shorter than    8413 bp
  85% of sequence in fragments shorter than    9749 bp
  90% of sequence in fragments shorter than   11571 bp
  95% of sequence in fragments shorter than   14757 bp

# BscGI.NheI (CCCGT+GCTAGC) double digest, 1517 thousand fragments (1231070+287384 sites), 1963 bp average fragment length
   5% of sequence in fragments shorter than     587 bp
  10% of sequence in fragments shorter than     999 bp
  15% of sequence in fragments shorter than    1379 bp
  20% of sequence in fragments shorter than    1724 bp
  25% of sequence in fragments shorter than    2073 bp
  30% of sequence in fragments shorter than    2449 bp
  35% of sequence in fragments shorter than    2829 bp
  40% of sequence in fragments shorter than    3236 bp
  45% of sequence in fragments shorter than    3665 bp
  50% of sequence in fragments shorter than    4126 bp
  55% of sequence in fragments shorter than    4628 bp
  60% of sequence in fragments shorter than    5183 bp
  65% of sequence in fragments shorter than    5799 bp
  70% of sequence in fragments shorter than    6508 bp
  75% of sequence in fragments shorter than    7338 bp
  80% of sequence in fragments shorter than    8355 bp
  85% of sequence in fragments shorter than    9638 bp
  90% of sequence in fragments shorter than   11448 bp
  95% of sequence in fragments shorter than   14534 bp

# AflIII.SalI (ACRYGT+GTCGAC) double digest, 1516 thousand fragments (1485394+31396 sites), 1965 bp average fragment length
   5% of sequence in fragments shorter than     710 bp
  10% of sequence in fragments shorter than    1106 bp
  15% of sequence in fragments shorter than    1443 bp
  20% of sequence in fragments shorter than    1761 bp
  25% of sequence in fragments shorter than    2064 bp
  30% of sequence in fragments shorter than    2363 bp
  35% of sequence in fragments shorter than    2673 bp
  40% of sequence in fragments shorter than    2984 bp
  45% of sequence in fragments shorter than    3314 bp
  50% of sequence in fragments shorter than    3670 bp
  55% of sequence in fragments shorter than    4052 bp
  60% of sequence in fragments shorter than    4467 bp
  65% of sequence in fragments shorter than    4930 bp
  70% of sequence in fragments shorter than    5458 bp
  75% of sequence in fragments shorter than    6055 bp
  80% of sequence in fragments shorter than    6771 bp
  85% of sequence in fragments shorter than    7692 bp
  90% of sequence in fragments shorter than    9016 bp
  95% of sequence in fragments shorter than   11491 bp

# BclI.BglII (TGATCA+AGATCT) double digest, 1513 thousand fragments (738785+774732 sites), 1969 bp average fragment length
   5% of sequence in fragments shorter than     691 bp
  10% of sequence in fragments shorter than    1026 bp
  15% of sequence in fragments shorter than    1331 bp
  20% of sequence in fragments shorter than    1602 bp
  25% of sequence in fragments shorter than    1874 bp
  30% of sequence in fragments shorter than    2152 bp
  35% of sequence in fragments shorter than    2433 bp
  40% of sequence in fragments shorter than    2722 bp
  45% of sequence in fragments shorter than    3030 bp
  50% of sequence in fragments shorter than    3352 bp
  55% of sequence in fragments shorter than    3699 bp
  60% of sequence in fragments shorter than    4079 bp
  65% of sequence in fragments shorter than    4501 bp
  70% of sequence in fragments shorter than    4984 bp
  75% of sequence in fragments shorter than    5557 bp
  80% of sequence in fragments shorter than    6261 bp
  85% of sequence in fragments shorter than    7174 bp
  90% of sequence in fragments shorter than    8531 bp
  95% of sequence in fragments shorter than   11330 bp

# AatII.FauI (GACGTC+CCCGC) double digest, 1510 thousand fragments (71089+1439772 sites), 1972 bp average fragment length
   5% of sequence in fragments shorter than     680 bp
  10% of sequence in fragments shorter than    1205 bp
  15% of sequence in fragments shorter than    1697 bp
  20% of sequence in fragments shorter than    2195 bp
  25% of sequence in fragments shorter than    2723 bp
  30% of sequence in fragments shorter than    3278 bp
  35% of sequence in fragments shorter than    3879 bp
  40% of sequence in fragments shorter than    4486 bp
  45% of sequence in fragments shorter than    5185 bp
  50% of sequence in fragments shorter than    5949 bp
  55% of sequence in fragments shorter than    6793 bp
  60% of sequence in fragments shorter than    7733 bp
  65% of sequence in fragments shorter than    8806 bp
  70% of sequence in fragments shorter than   10062 bp
  75% of sequence in fragments shorter than   11541 bp
  80% of sequence in fragments shorter than   13364 bp
  85% of sequence in fragments shorter than   15827 bp
  90% of sequence in fragments shorter than   19373 bp
  95% of sequence in fragments shorter than   26180 bp

# AatII.BspMI (GACGTC+ACCTGC) double digest, 1508 thousand fragments (71089+1438129 sites), 1975 bp average fragment length
   5% of sequence in fragments shorter than     667 bp
  10% of sequence in fragments shorter than    1029 bp
  15% of sequence in fragments shorter than    1366 bp
  20% of sequence in fragments shorter than    1680 bp
  25% of sequence in fragments shorter than    1985 bp
  30% of sequence in fragments shorter than    2291 bp
  35% of sequence in fragments shorter than    2621 bp
  40% of sequence in fragments shorter than    2958 bp
  45% of sequence in fragments shorter than    3278 bp
  50% of sequence in fragments shorter than    3651 bp
  55% of sequence in fragments shorter than    4067 bp
  60% of sequence in fragments shorter than    4523 bp
  65% of sequence in fragments shorter than    5028 bp
  70% of sequence in fragments shorter than    5602 bp
  75% of sequence in fragments shorter than    6270 bp
  80% of sequence in fragments shorter than    7084 bp
  85% of sequence in fragments shorter than    8137 bp
  90% of sequence in fragments shorter than    9585 bp
  95% of sequence in fragments shorter than   12181 bp

# BglII.FnuDII (AGATCT+CGCG) double digest, 1508 thousand fragments (774732+733940 sites), 1975 bp average fragment length
   5% of sequence in fragments shorter than     757 bp
  10% of sequence in fragments shorter than    1215 bp
  15% of sequence in fragments shorter than    1601 bp
  20% of sequence in fragments shorter than    1963 bp
  25% of sequence in fragments shorter than    2323 bp
  30% of sequence in fragments shorter than    2684 bp
  35% of sequence in fragments shorter than    3055 bp
  40% of sequence in fragments shorter than    3436 bp
  45% of sequence in fragments shorter than    3832 bp
  50% of sequence in fragments shorter than    4252 bp
  55% of sequence in fragments shorter than    4702 bp
  60% of sequence in fragments shorter than    5188 bp
  65% of sequence in fragments shorter than    5742 bp
  70% of sequence in fragments shorter than    6364 bp
  75% of sequence in fragments shorter than    7099 bp
  80% of sequence in fragments shorter than    7997 bp
  85% of sequence in fragments shorter than    9155 bp
  90% of sequence in fragments shorter than   10823 bp
  95% of sequence in fragments shorter than   14162 bp

# FauI.SacII (CCCGC+CCGCGG) double digest, 1506 thousand fragments (1439772+66901 sites), 1978 bp average fragment length
   5% of sequence in fragments shorter than     704 bp
  10% of sequence in fragments shorter than    1260 bp
  15% of sequence in fragments shorter than    1781 bp
  20% of sequence in fragments shorter than    2315 bp
  25% of sequence in fragments shorter than    2880 bp
  30% of sequence in fragments shorter than    3476 bp
  35% of sequence in fragments shorter than    4118 bp
  40% of sequence in fragments shorter than    4772 bp
  45% of sequence in fragments shorter than    5524 bp
  50% of sequence in fragments shorter than    6355 bp
  55% of sequence in fragments shorter than    7263 bp
  60% of sequence in fragments shorter than    8288 bp
  65% of sequence in fragments shorter than    9461 bp
  70% of sequence in fragments shorter than   10814 bp
  75% of sequence in fragments shorter than   12431 bp
  80% of sequence in fragments shorter than   14420 bp
  85% of sequence in fragments shorter than   17095 bp
  90% of sequence in fragments shorter than   21010 bp
  95% of sequence in fragments shorter than   28472 bp

# NdeI.SacI (CATATG+GAGCTC) double digest, 1506 thousand fragments (903360+603323 sites), 1978 bp average fragment length
   5% of sequence in fragments shorter than     699 bp
  10% of sequence in fragments shorter than    1052 bp
  15% of sequence in fragments shorter than    1345 bp
  20% of sequence in fragments shorter than    1634 bp
  25% of sequence in fragments shorter than    1915 bp
  30% of sequence in fragments shorter than    2194 bp
  35% of sequence in fragments shorter than    2485 bp
  40% of sequence in fragments shorter than    2757 bp
  45% of sequence in fragments shorter than    3067 bp
  50% of sequence in fragments shorter than    3391 bp
  55% of sequence in fragments shorter than    3743 bp
  60% of sequence in fragments shorter than    4124 bp
  65% of sequence in fragments shorter than    4547 bp
  70% of sequence in fragments shorter than    5029 bp
  75% of sequence in fragments shorter than    5588 bp
  80% of sequence in fragments shorter than    6264 bp
  85% of sequence in fragments shorter than    7153 bp
  90% of sequence in fragments shorter than    8410 bp
  95% of sequence in fragments shorter than   10844 bp

# AvaIII.HgaI (ATGCAT+GACGC) double digest, 1504 thousand fragments (933357+571889 sites), 1980 bp average fragment length
   5% of sequence in fragments shorter than     696 bp
  10% of sequence in fragments shorter than    1077 bp
  15% of sequence in fragments shorter than    1405 bp
  20% of sequence in fragments shorter than    1720 bp
  25% of sequence in fragments shorter than    2021 bp
  30% of sequence in fragments shorter than    2315 bp
  35% of sequence in fragments shorter than    2624 bp
  40% of sequence in fragments shorter than    2934 bp
  45% of sequence in fragments shorter than    3267 bp
  50% of sequence in fragments shorter than    3619 bp
  55% of sequence in fragments shorter than    3998 bp
  60% of sequence in fragments shorter than    4410 bp
  65% of sequence in fragments shorter than    4869 bp
  70% of sequence in fragments shorter than    5393 bp
  75% of sequence in fragments shorter than    6002 bp
  80% of sequence in fragments shorter than    6725 bp
  85% of sequence in fragments shorter than    7664 bp
  90% of sequence in fragments shorter than    8999 bp
  95% of sequence in fragments shorter than   11448 bp

# BspMI.SacII (ACCTGC+CCGCGG) double digest, 1504 thousand fragments (1438129+66901 sites), 1980 bp average fragment length
   5% of sequence in fragments shorter than     667 bp
  10% of sequence in fragments shorter than    1035 bp
  15% of sequence in fragments shorter than    1381 bp
  20% of sequence in fragments shorter than    1709 bp
  25% of sequence in fragments shorter than    2013 bp
  30% of sequence in fragments shorter than    2340 bp
  35% of sequence in fragments shorter than    2681 bp
  40% of sequence in fragments shorter than    3030 bp
  45% of sequence in fragments shorter than    3352 bp
  50% of sequence in fragments shorter than    3746 bp
  55% of sequence in fragments shorter than    4173 bp
  60% of sequence in fragments shorter than    4643 bp
  65% of sequence in fragments shorter than    5169 bp
  70% of sequence in fragments shorter than    5759 bp
  75% of sequence in fragments shorter than    6449 bp
  80% of sequence in fragments shorter than    7292 bp
  85% of sequence in fragments shorter than    8382 bp
  90% of sequence in fragments shorter than    9888 bp
  95% of sequence in fragments shorter than   12596 bp

# AvaIII.EciI (ATGCAT+GGCGGA) double digest, 1504 thousand fragments (933357+571273 sites), 1981 bp average fragment length
   5% of sequence in fragments shorter than     688 bp
  10% of sequence in fragments shorter than    1061 bp
  15% of sequence in fragments shorter than    1383 bp
  20% of sequence in fragments shorter than    1686 bp
  25% of sequence in fragments shorter than    1983 bp
  30% of sequence in fragments shorter than    2270 bp
  35% of sequence in fragments shorter than    2574 bp
  40% of sequence in fragments shorter than    2886 bp
  45% of sequence in fragments shorter than    3215 bp
  50% of sequence in fragments shorter than    3562 bp
  55% of sequence in fragments shorter than    3945 bp
  60% of sequence in fragments shorter than    4362 bp
  65% of sequence in fragments shorter than    4830 bp
  70% of sequence in fragments shorter than    5357 bp
  75% of sequence in fragments shorter than    5982 bp
  80% of sequence in fragments shorter than    6745 bp
  85% of sequence in fragments shorter than    7732 bp
  90% of sequence in fragments shorter than    9166 bp
  95% of sequence in fragments shorter than   11910 bp

# AflII.HindIII (CTTAAG+AAGCTT) double digest, 1503 thousand fragments (643284+860365 sites), 1982 bp average fragment length
   5% of sequence in fragments shorter than     687 bp
  10% of sequence in fragments shorter than    1060 bp
  15% of sequence in fragments shorter than    1376 bp
  20% of sequence in fragments shorter than    1678 bp
  25% of sequence in fragments shorter than    1941 bp
  30% of sequence in fragments shorter than    2228 bp
  35% of sequence in fragments shorter than    2516 bp
  40% of sequence in fragments shorter than    2817 bp
  45% of sequence in fragments shorter than    3132 bp
  50% of sequence in fragments shorter than    3472 bp
  55% of sequence in fragments shorter than    3836 bp
  60% of sequence in fragments shorter than    4239 bp
  65% of sequence in fragments shorter than    4685 bp
  70% of sequence in fragments shorter than    5196 bp
  75% of sequence in fragments shorter than    5794 bp
  80% of sequence in fragments shorter than    6538 bp
  85% of sequence in fragments shorter than    7519 bp
  90% of sequence in fragments shorter than    8962 bp
  95% of sequence in fragments shorter than   11858 bp

# ApaLI.AvaI (GTGCAC+CYCGRG) double digest, 1501 thousand fragments (496255+1005714 sites), 1984 bp average fragment length
   5% of sequence in fragments shorter than     655 bp
  10% of sequence in fragments shorter than    1053 bp
  15% of sequence in fragments shorter than    1423 bp
  20% of sequence in fragments shorter than    1786 bp
  25% of sequence in fragments shorter than    2153 bp
  30% of sequence in fragments shorter than    2537 bp
  35% of sequence in fragments shorter than    2938 bp
  40% of sequence in fragments shorter than    3366 bp
  45% of sequence in fragments shorter than    3821 bp
  50% of sequence in fragments shorter than    4321 bp
  55% of sequence in fragments shorter than    4810 bp
  60% of sequence in fragments shorter than    5405 bp
  65% of sequence in fragments shorter than    6068 bp
  70% of sequence in fragments shorter than    6867 bp
  75% of sequence in fragments shorter than    7810 bp
  80% of sequence in fragments shorter than    8968 bp
  85% of sequence in fragments shorter than   10502 bp
  90% of sequence in fragments shorter than   12800 bp
  95% of sequence in fragments shorter than   17239 bp

# AccI.SpeI (GTMKAC+ACTAGT) double digest, 1500 thousand fragments (1101063+400286 sites), 1985 bp average fragment length
   5% of sequence in fragments shorter than     684 bp
  10% of sequence in fragments shorter than    1049 bp
  15% of sequence in fragments shorter than    1363 bp
  20% of sequence in fragments shorter than    1655 bp
  25% of sequence in fragments shorter than    1942 bp
  30% of sequence in fragments shorter than    2230 bp
  35% of sequence in fragments shorter than    2524 bp
  40% of sequence in fragments shorter than    2827 bp
  45% of sequence in fragments shorter than    3151 bp
  50% of sequence in fragments shorter than    3493 bp
  55% of sequence in fragments shorter than    3863 bp
  60% of sequence in fragments shorter than    4268 bp
  65% of sequence in fragments shorter than    4718 bp
  70% of sequence in fragments shorter than    5213 bp
  75% of sequence in fragments shorter than    5760 bp
  80% of sequence in fragments shorter than    6488 bp
  85% of sequence in fragments shorter than    7439 bp
  90% of sequence in fragments shorter than    8819 bp
  95% of sequence in fragments shorter than   11420 bp

# AflIII.SgrAI (ACRYGT+CRCCGGYG) double digest, 1500 thousand fragments (1485394+15270 sites), 1986 bp average fragment length
   5% of sequence in fragments shorter than     717 bp
  10% of sequence in fragments shorter than    1119 bp
  15% of sequence in fragments shorter than    1460 bp
  20% of sequence in fragments shorter than    1782 bp
  25% of sequence in fragments shorter than    2086 bp
  30% of sequence in fragments shorter than    2390 bp
  35% of sequence in fragments shorter than    2704 bp
  40% of sequence in fragments shorter than    3018 bp
  45% of sequence in fragments shorter than    3352 bp
  50% of sequence in fragments shorter than    3712 bp
  55% of sequence in fragments shorter than    4099 bp
  60% of sequence in fragments shorter than    4521 bp
  65% of sequence in fragments shorter than    4992 bp
  70% of sequence in fragments shorter than    5529 bp
  75% of sequence in fragments shorter than    6134 bp
  80% of sequence in fragments shorter than    6857 bp
  85% of sequence in fragments shorter than    7792 bp
  90% of sequence in fragments shorter than    9129 bp
  95% of sequence in fragments shorter than   11632 bp

# AflIII.NruI (ACRYGT+TCGCGA) double digest, 1499 thousand fragments (1485394+14872 sites), 1986 bp average fragment length
   5% of sequence in fragments shorter than     718 bp
  10% of sequence in fragments shorter than    1119 bp
  15% of sequence in fragments shorter than    1461 bp
  20% of sequence in fragments shorter than    1782 bp
  25% of sequence in fragments shorter than    2086 bp
  30% of sequence in fragments shorter than    2391 bp
  35% of sequence in fragments shorter than    2705 bp
  40% of sequence in fragments shorter than    3018 bp
  45% of sequence in fragments shorter than    3353 bp
  50% of sequence in fragments shorter than    3712 bp
  55% of sequence in fragments shorter than    4100 bp
  60% of sequence in fragments shorter than    4521 bp
  65% of sequence in fragments shorter than    4989 bp
  70% of sequence in fragments shorter than    5527 bp
  75% of sequence in fragments shorter than    6131 bp
  80% of sequence in fragments shorter than    6854 bp
  85% of sequence in fragments shorter than    7789 bp
  90% of sequence in fragments shorter than    9125 bp
  95% of sequence in fragments shorter than   11608 bp

# AflIII.PvuI (ACRYGT+CGATCG) double digest, 1499 thousand fragments (1485394+14186 sites), 1987 bp average fragment length
   5% of sequence in fragments shorter than     718 bp
  10% of sequence in fragments shorter than    1120 bp
  15% of sequence in fragments shorter than    1462 bp
  20% of sequence in fragments shorter than    1783 bp
  25% of sequence in fragments shorter than    2086 bp
  30% of sequence in fragments shorter than    2390 bp
  35% of sequence in fragments shorter than    2704 bp
  40% of sequence in fragments shorter than    3018 bp
  45% of sequence in fragments shorter than    3353 bp
  50% of sequence in fragments shorter than    3713 bp
  55% of sequence in fragments shorter than    4101 bp
  60% of sequence in fragments shorter than    4522 bp
  65% of sequence in fragments shorter than    4990 bp
  70% of sequence in fragments shorter than    5528 bp
  75% of sequence in fragments shorter than    6131 bp
  80% of sequence in fragments shorter than    6854 bp
  85% of sequence in fragments shorter than    7788 bp
  90% of sequence in fragments shorter than    9120 bp
  95% of sequence in fragments shorter than   11588 bp

# AflIII.FseI (ACRYGT+GGCCGGCC) double digest, 1498 thousand fragments (1485394+13662 sites), 1988 bp average fragment length
   5% of sequence in fragments shorter than     718 bp
  10% of sequence in fragments shorter than    1120 bp
  15% of sequence in fragments shorter than    1461 bp
  20% of sequence in fragments shorter than    1784 bp
  25% of sequence in fragments shorter than    2087 bp
  30% of sequence in fragments shorter than    2393 bp
  35% of sequence in fragments shorter than    2707 bp
  40% of sequence in fragments shorter than    3020 bp
  45% of sequence in fragments shorter than    3355 bp
  50% of sequence in fragments shorter than    3715 bp
  55% of sequence in fragments shorter than    4104 bp
  60% of sequence in fragments shorter than    4525 bp
  65% of sequence in fragments shorter than    4994 bp
  70% of sequence in fragments shorter than    5533 bp
  75% of sequence in fragments shorter than    6138 bp
  80% of sequence in fragments shorter than    6861 bp
  85% of sequence in fragments shorter than    7794 bp
  90% of sequence in fragments shorter than    9131 bp
  95% of sequence in fragments shorter than   11625 bp

# FauI.PspXI (CCCGC+VCTCGAGB) double digest, 1498 thousand fragments (1439772+59193 sites), 1988 bp average fragment length
   5% of sequence in fragments shorter than     685 bp
  10% of sequence in fragments shorter than    1213 bp
  15% of sequence in fragments shorter than    1713 bp
  20% of sequence in fragments shorter than    2218 bp
  25% of sequence in fragments shorter than    2756 bp
  30% of sequence in fragments shorter than    3319 bp
  35% of sequence in fragments shorter than    3933 bp
  40% of sequence in fragments shorter than    4554 bp
  45% of sequence in fragments shorter than    5266 bp
  50% of sequence in fragments shorter than    6045 bp
  55% of sequence in fragments shorter than    6907 bp
  60% of sequence in fragments shorter than    7873 bp
  65% of sequence in fragments shorter than    8972 bp
  70% of sequence in fragments shorter than   10266 bp
  75% of sequence in fragments shorter than   11784 bp
  80% of sequence in fragments shorter than   13648 bp
  85% of sequence in fragments shorter than   16167 bp
  90% of sequence in fragments shorter than   19784 bp
  95% of sequence in fragments shorter than   26853 bp

# BclI.NcoI (TGATCA+CCATGG) double digest, 1497 thousand fragments (738785+759594 sites), 1989 bp average fragment length
   5% of sequence in fragments shorter than     699 bp
  10% of sequence in fragments shorter than    1054 bp
  15% of sequence in fragments shorter than    1366 bp
  20% of sequence in fragments shorter than    1649 bp
  25% of sequence in fragments shorter than    1929 bp
  30% of sequence in fragments shorter than    2189 bp
  35% of sequence in fragments shorter than    2471 bp
  40% of sequence in fragments shorter than    2759 bp
  45% of sequence in fragments shorter than    3064 bp
  50% of sequence in fragments shorter than    3393 bp
  55% of sequence in fragments shorter than    3746 bp
  60% of sequence in fragments shorter than    4129 bp
  65% of sequence in fragments shorter than    4545 bp
  70% of sequence in fragments shorter than    5017 bp
  75% of sequence in fragments shorter than    5574 bp
  80% of sequence in fragments shorter than    6249 bp
  85% of sequence in fragments shorter than    7130 bp
  90% of sequence in fragments shorter than    8400 bp
  95% of sequence in fragments shorter than   10924 bp

# AvrII.NdeI (CCTAGG+CATATG) double digest, 1497 thousand fragments (594956+903360 sites), 1989 bp average fragment length
   5% of sequence in fragments shorter than     676 bp
  10% of sequence in fragments shorter than    1052 bp
  15% of sequence in fragments shorter than    1368 bp
  20% of sequence in fragments shorter than    1678 bp
  25% of sequence in fragments shorter than    1979 bp
  30% of sequence in fragments shorter than    2280 bp
  35% of sequence in fragments shorter than    2589 bp
  40% of sequence in fragments shorter than    2903 bp
  45% of sequence in fragments shorter than    3231 bp
  50% of sequence in fragments shorter than    3582 bp
  55% of sequence in fragments shorter than    3953 bp
  60% of sequence in fragments shorter than    4360 bp
  65% of sequence in fragments shorter than    4801 bp
  70% of sequence in fragments shorter than    5301 bp
  75% of sequence in fragments shorter than    5884 bp
  80% of sequence in fragments shorter than    6592 bp
  85% of sequence in fragments shorter than    7536 bp
  90% of sequence in fragments shorter than    8903 bp
  95% of sequence in fragments shorter than   11640 bp

# AvaIII.MfeI (ATGCAT+CAATTG) double digest, 1497 thousand fragments (933357+564303 sites), 1990 bp average fragment length
   5% of sequence in fragments shorter than     695 bp
  10% of sequence in fragments shorter than    1053 bp
  15% of sequence in fragments shorter than    1375 bp
  20% of sequence in fragments shorter than    1678 bp
  25% of sequence in fragments shorter than    1967 bp
  30% of sequence in fragments shorter than    2250 bp
  35% of sequence in fragments shorter than    2552 bp
  40% of sequence in fragments shorter than    2862 bp
  45% of sequence in fragments shorter than    3191 bp
  50% of sequence in fragments shorter than    3543 bp
  55% of sequence in fragments shorter than    3930 bp
  60% of sequence in fragments shorter than    4353 bp
  65% of sequence in fragments shorter than    4824 bp
  70% of sequence in fragments shorter than    5356 bp
  75% of sequence in fragments shorter than    5997 bp
  80% of sequence in fragments shorter than    6795 bp
  85% of sequence in fragments shorter than    7862 bp
  90% of sequence in fragments shorter than    9460 bp
  95% of sequence in fragments shorter than   12639 bp

# HgaI.StuI (GACGC+AGGCCT) double digest, 1497 thousand fragments (571889+925729 sites), 1990 bp average fragment length
   5% of sequence in fragments shorter than     620 bp
  10% of sequence in fragments shorter than     993 bp
  15% of sequence in fragments shorter than    1378 bp
  20% of sequence in fragments shorter than    1756 bp
  25% of sequence in fragments shorter than    2129 bp
  30% of sequence in fragments shorter than    2534 bp
  35% of sequence in fragments shorter than    2950 bp
  40% of sequence in fragments shorter than    3379 bp
  45% of sequence in fragments shorter than    3832 bp
  50% of sequence in fragments shorter than    4320 bp
  55% of sequence in fragments shorter than    4847 bp
  60% of sequence in fragments shorter than    5426 bp
  65% of sequence in fragments shorter than    6070 bp
  70% of sequence in fragments shorter than    6807 bp
  75% of sequence in fragments shorter than    7647 bp
  80% of sequence in fragments shorter than    8668 bp
  85% of sequence in fragments shorter than   10002 bp
  90% of sequence in fragments shorter than   11823 bp
  95% of sequence in fragments shorter than   14859 bp

# BspMI.PspXI (ACCTGC+VCTCGAGB) double digest, 1496 thousand fragments (1438129+59193 sites), 1990 bp average fragment length
   5% of sequence in fragments shorter than     672 bp
  10% of sequence in fragments shorter than    1034 bp
  15% of sequence in fragments shorter than    1376 bp
  20% of sequence in fragments shorter than    1692 bp
  25% of sequence in fragments shorter than    1992 bp
  30% of sequence in fragments shorter than    2308 bp
  35% of sequence in fragments shorter than    2641 bp
  40% of sequence in fragments shorter than    2983 bp
  45% of sequence in fragments shorter than    3302 bp
  50% of sequence in fragments shorter than    3683 bp
  55% of sequence in fragments shorter than    4104 bp
  60% of sequence in fragments shorter than    4566 bp
  65% of sequence in fragments shorter than    5079 bp
  70% of sequence in fragments shorter than    5657 bp
  75% of sequence in fragments shorter than    6333 bp
  80% of sequence in fragments shorter than    7157 bp
  85% of sequence in fragments shorter than    8218 bp
  90% of sequence in fragments shorter than    9698 bp
  95% of sequence in fragments shorter than   12323 bp

# EciI.StuI (GGCGGA+AGGCCT) double digest, 1496 thousand fragments (571273+925729 sites), 1991 bp average fragment length
   5% of sequence in fragments shorter than     629 bp
  10% of sequence in fragments shorter than     992 bp
  15% of sequence in fragments shorter than    1357 bp
  20% of sequence in fragments shorter than    1700 bp
  25% of sequence in fragments shorter than    2040 bp
  30% of sequence in fragments shorter than    2413 bp
  35% of sequence in fragments shorter than    2795 bp
  40% of sequence in fragments shorter than    3200 bp
  45% of sequence in fragments shorter than    3629 bp
  50% of sequence in fragments shorter than    4095 bp
  55% of sequence in fragments shorter than    4599 bp
  60% of sequence in fragments shorter than    5161 bp
  65% of sequence in fragments shorter than    5786 bp
  70% of sequence in fragments shorter than    6501 bp
  75% of sequence in fragments shorter than    7341 bp
  80% of sequence in fragments shorter than    8346 bp
  85% of sequence in fragments shorter than    9656 bp
  90% of sequence in fragments shorter than   11481 bp
  95% of sequence in fragments shorter than   14641 bp

# AflIII.NotI (ACRYGT+GCGGCCGC) double digest, 1494 thousand fragments (1485394+9795 sites), 1993 bp average fragment length
   5% of sequence in fragments shorter than     720 bp
  10% of sequence in fragments shorter than    1123 bp
  15% of sequence in fragments shorter than    1467 bp
  20% of sequence in fragments shorter than    1790 bp
  25% of sequence in fragments shorter than    2091 bp
  30% of sequence in fragments shorter than    2400 bp
  35% of sequence in fragments shorter than    2715 bp
  40% of sequence in fragments shorter than    3030 bp
  45% of sequence in fragments shorter than    3367 bp
  50% of sequence in fragments shorter than    3729 bp
  55% of sequence in fragments shorter than    4118 bp
  60% of sequence in fragments shorter than    4542 bp
  65% of sequence in fragments shorter than    5014 bp
  70% of sequence in fragments shorter than    5554 bp
  75% of sequence in fragments shorter than    6161 bp
  80% of sequence in fragments shorter than    6890 bp
  85% of sequence in fragments shorter than    7825 bp
  90% of sequence in fragments shorter than    9173 bp
  95% of sequence in fragments shorter than   11697 bp

# AflIII.RsrII (ACRYGT+CGGWCCG) double digest, 1494 thousand fragments (1485394+9764 sites), 1993 bp average fragment length
   5% of sequence in fragments shorter than     720 bp
  10% of sequence in fragments shorter than    1123 bp
  15% of sequence in fragments shorter than    1466 bp
  20% of sequence in fragments shorter than    1789 bp
  25% of sequence in fragments shorter than    2090 bp
  30% of sequence in fragments shorter than    2400 bp
  35% of sequence in fragments shorter than    2715 bp
  40% of sequence in fragments shorter than    3030 bp
  45% of sequence in fragments shorter than    3367 bp
  50% of sequence in fragments shorter than    3728 bp
  55% of sequence in fragments shorter than    4117 bp
  60% of sequence in fragments shorter than    4539 bp
  65% of sequence in fragments shorter than    5011 bp
  70% of sequence in fragments shorter than    5549 bp
  75% of sequence in fragments shorter than    6155 bp
  80% of sequence in fragments shorter than    6884 bp
  85% of sequence in fragments shorter than    7820 bp
  90% of sequence in fragments shorter than    9161 bp
  95% of sequence in fragments shorter than   11671 bp

# AflII.XbaI (CTTAAG+TCTAGA) double digest, 1493 thousand fragments (643284+850998 sites), 1994 bp average fragment length
   5% of sequence in fragments shorter than     700 bp
  10% of sequence in fragments shorter than    1037 bp
  15% of sequence in fragments shorter than    1349 bp
  20% of sequence in fragments shorter than    1626 bp
  25% of sequence in fragments shorter than    1909 bp
  30% of sequence in fragments shorter than    2205 bp
  35% of sequence in fragments shorter than    2502 bp
  40% of sequence in fragments shorter than    2808 bp
  45% of sequence in fragments shorter than    3126 bp
  50% of sequence in fragments shorter than    3469 bp
  55% of sequence in fragments shorter than    3833 bp
  60% of sequence in fragments shorter than    4233 bp
  65% of sequence in fragments shorter than    4674 bp
  70% of sequence in fragments shorter than    5176 bp
  75% of sequence in fragments shorter than    5765 bp
  80% of sequence in fragments shorter than    6496 bp
  85% of sequence in fragments shorter than    7449 bp
  90% of sequence in fragments shorter than    8810 bp
  95% of sequence in fragments shorter than   11395 bp

# AclI.PstI (AACGTT+CTGCAG) double digest, 1493 thousand fragments (172706+1321469 sites), 1995 bp average fragment length
   5% of sequence in fragments shorter than     669 bp
  10% of sequence in fragments shorter than    1034 bp
  15% of sequence in fragments shorter than    1367 bp
  20% of sequence in fragments shorter than    1682 bp
  25% of sequence in fragments shorter than    1999 bp
  30% of sequence in fragments shorter than    2312 bp
  35% of sequence in fragments shorter than    2648 bp
  40% of sequence in fragments shorter than    2995 bp
  45% of sequence in fragments shorter than    3361 bp
  50% of sequence in fragments shorter than    3735 bp
  55% of sequence in fragments shorter than    4157 bp
  60% of sequence in fragments shorter than    4630 bp
  65% of sequence in fragments shorter than    5147 bp
  70% of sequence in fragments shorter than    5733 bp
  75% of sequence in fragments shorter than    6420 bp
  80% of sequence in fragments shorter than    7263 bp
  85% of sequence in fragments shorter than    8338 bp
  90% of sequence in fragments shorter than    9878 bp
  95% of sequence in fragments shorter than   12627 bp

# FnuDII.NcoI (CGCG+CCATGG) double digest, 1493 thousand fragments (733940+759594 sites), 1995 bp average fragment length
   5% of sequence in fragments shorter than     744 bp
  10% of sequence in fragments shorter than    1224 bp
  15% of sequence in fragments shorter than    1642 bp
  20% of sequence in fragments shorter than    2049 bp
  25% of sequence in fragments shorter than    2450 bp
  30% of sequence in fragments shorter than    2854 bp
  35% of sequence in fragments shorter than    3276 bp
  40% of sequence in fragments shorter than    3712 bp
  45% of sequence in fragments shorter than    4176 bp
  50% of sequence in fragments shorter than    4647 bp
  55% of sequence in fragments shorter than    5172 bp
  60% of sequence in fragments shorter than    5713 bp
  65% of sequence in fragments shorter than    6351 bp
  70% of sequence in fragments shorter than    7098 bp
  75% of sequence in fragments shorter than    7983 bp
  80% of sequence in fragments shorter than    9059 bp
  85% of sequence in fragments shorter than   10474 bp
  90% of sequence in fragments shorter than   12509 bp
  95% of sequence in fragments shorter than   16492 bp

# AgeI.FauI (ACCGGT+CCCGC) double digest, 1492 thousand fragments (53386+1439772 sites), 1996 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1222 bp
  15% of sequence in fragments shorter than    1713 bp
  20% of sequence in fragments shorter than    2216 bp
  25% of sequence in fragments shorter than    2738 bp
  30% of sequence in fragments shorter than    3298 bp
  35% of sequence in fragments shorter than    3904 bp
  40% of sequence in fragments shorter than    4534 bp
  45% of sequence in fragments shorter than    5236 bp
  50% of sequence in fragments shorter than    6007 bp
  55% of sequence in fragments shorter than    6866 bp
  60% of sequence in fragments shorter than    7817 bp
  65% of sequence in fragments shorter than    8899 bp
  70% of sequence in fragments shorter than   10177 bp
  75% of sequence in fragments shorter than   11661 bp
  80% of sequence in fragments shorter than   13517 bp
  85% of sequence in fragments shorter than   16016 bp
  90% of sequence in fragments shorter than   19652 bp
  95% of sequence in fragments shorter than   26628 bp

# AgeI.BspMI (ACCGGT+ACCTGC) double digest, 1491 thousand fragments (53386+1438129 sites), 1998 bp average fragment length
   5% of sequence in fragments shorter than     678 bp
  10% of sequence in fragments shorter than    1036 bp
  15% of sequence in fragments shorter than    1374 bp
  20% of sequence in fragments shorter than    1691 bp
  25% of sequence in fragments shorter than    1997 bp
  30% of sequence in fragments shorter than    2317 bp
  35% of sequence in fragments shorter than    2650 bp
  40% of sequence in fragments shorter than    2990 bp
  45% of sequence in fragments shorter than    3308 bp
  50% of sequence in fragments shorter than    3691 bp
  55% of sequence in fragments shorter than    4109 bp
  60% of sequence in fragments shorter than    4565 bp
  65% of sequence in fragments shorter than    5076 bp
  70% of sequence in fragments shorter than    5654 bp
  75% of sequence in fragments shorter than    6328 bp
  80% of sequence in fragments shorter than    7154 bp
  85% of sequence in fragments shorter than    8212 bp
  90% of sequence in fragments shorter than    9688 bp
  95% of sequence in fragments shorter than   12323 bp

# AflII.EcoRI (CTTAAG+GAATTC) double digest, 1490 thousand fragments (643284+847401 sites), 1999 bp average fragment length
   5% of sequence in fragments shorter than     688 bp
  10% of sequence in fragments shorter than    1038 bp
  15% of sequence in fragments shorter than    1373 bp
  20% of sequence in fragments shorter than    1675 bp
  25% of sequence in fragments shorter than    1973 bp
  30% of sequence in fragments shorter than    2266 bp
  35% of sequence in fragments shorter than    2566 bp
  40% of sequence in fragments shorter than    2868 bp
  45% of sequence in fragments shorter than    3191 bp
  50% of sequence in fragments shorter than    3525 bp
  55% of sequence in fragments shorter than    3892 bp
  60% of sequence in fragments shorter than    4286 bp
  65% of sequence in fragments shorter than    4732 bp
  70% of sequence in fragments shorter than    5232 bp
  75% of sequence in fragments shorter than    5823 bp
  80% of sequence in fragments shorter than    6547 bp
  85% of sequence in fragments shorter than    7487 bp
  90% of sequence in fragments shorter than    8820 bp
  95% of sequence in fragments shorter than   11395 bp

# AccI.HpaI (GTMKAC+GTTAAC) double digest, 1490 thousand fragments (1101063+389418 sites), 1999 bp average fragment length
   5% of sequence in fragments shorter than     702 bp
  10% of sequence in fragments shorter than    1066 bp
  15% of sequence in fragments shorter than    1376 bp
  20% of sequence in fragments shorter than    1667 bp
  25% of sequence in fragments shorter than    1950 bp
  30% of sequence in fragments shorter than    2234 bp
  35% of sequence in fragments shorter than    2525 bp
  40% of sequence in fragments shorter than    2820 bp
  45% of sequence in fragments shorter than    3138 bp
  50% of sequence in fragments shorter than    3474 bp
  55% of sequence in fragments shorter than    3817 bp
  60% of sequence in fragments shorter than    4217 bp
  65% of sequence in fragments shorter than    4662 bp
  70% of sequence in fragments shorter than    5168 bp
  75% of sequence in fragments shorter than    5752 bp
  80% of sequence in fragments shorter than    6433 bp
  85% of sequence in fragments shorter than    7362 bp
  90% of sequence in fragments shorter than    8720 bp
  95% of sequence in fragments shorter than   11289 bp

# HaeII.HindIII (RGCGCY+AAGCTT) double digest, 1490 thousand fragments (630101+860365 sites), 1999 bp average fragment length
   5% of sequence in fragments shorter than     674 bp
  10% of sequence in fragments shorter than    1041 bp
  15% of sequence in fragments shorter than    1398 bp
  20% of sequence in fragments shorter than    1738 bp
  25% of sequence in fragments shorter than    2041 bp
  30% of sequence in fragments shorter than    2364 bp
  35% of sequence in fragments shorter than    2695 bp
  40% of sequence in fragments shorter than    3030 bp
  45% of sequence in fragments shorter than    3385 bp
  50% of sequence in fragments shorter than    3758 bp
  55% of sequence in fragments shorter than    4155 bp
  60% of sequence in fragments shorter than    4582 bp
  65% of sequence in fragments shorter than    5053 bp
  70% of sequence in fragments shorter than    5587 bp
  75% of sequence in fragments shorter than    6214 bp
  80% of sequence in fragments shorter than    6958 bp
  85% of sequence in fragments shorter than    7900 bp
  90% of sequence in fragments shorter than    9193 bp
  95% of sequence in fragments shorter than   11402 bp

# AflIII.AscI (ACRYGT+GGCGCGCC) double digest, 1489 thousand fragments (1485394+4751 sites), 2000 bp average fragment length
   5% of sequence in fragments shorter than     723 bp
  10% of sequence in fragments shorter than    1127 bp
  15% of sequence in fragments shorter than    1471 bp
  20% of sequence in fragments shorter than    1796 bp
  25% of sequence in fragments shorter than    2098 bp
  30% of sequence in fragments shorter than    2409 bp
  35% of sequence in fragments shorter than    2725 bp
  40% of sequence in fragments shorter than    3041 bp
  45% of sequence in fragments shorter than    3379 bp
  50% of sequence in fragments shorter than    3742 bp
  55% of sequence in fragments shorter than    4133 bp
  60% of sequence in fragments shorter than    4558 bp
  65% of sequence in fragments shorter than    5032 bp
  70% of sequence in fragments shorter than    5573 bp
  75% of sequence in fragments shorter than    6183 bp
  80% of sequence in fragments shorter than    6912 bp
  85% of sequence in fragments shorter than    7858 bp
  90% of sequence in fragments shorter than    9210 bp
  95% of sequence in fragments shorter than   11753 bp

# MfeI.StuI (CAATTG+AGGCCT) double digest, 1489 thousand fragments (564303+925729 sites), 2000 bp average fragment length
   5% of sequence in fragments shorter than     664 bp
  10% of sequence in fragments shorter than    1029 bp
  15% of sequence in fragments shorter than    1380 bp
  20% of sequence in fragments shorter than    1701 bp
  25% of sequence in fragments shorter than    2002 bp
  30% of sequence in fragments shorter than    2322 bp
  35% of sequence in fragments shorter than    2636 bp
  40% of sequence in fragments shorter than    2968 bp
  45% of sequence in fragments shorter than    3313 bp
  50% of sequence in fragments shorter than    3678 bp
  55% of sequence in fragments shorter than    4070 bp
  60% of sequence in fragments shorter than    4491 bp
  65% of sequence in fragments shorter than    4955 bp
  70% of sequence in fragments shorter than    5484 bp
  75% of sequence in fragments shorter than    6087 bp
  80% of sequence in fragments shorter than    6815 bp
  85% of sequence in fragments shorter than    7736 bp
  90% of sequence in fragments shorter than    9022 bp
  95% of sequence in fragments shorter than   11205 bp

# BglII.BsaAI (AGATCT+YACGTR) double digest, 1488 thousand fragments (774732+713801 sites), 2002 bp average fragment length
   5% of sequence in fragments shorter than     715 bp
  10% of sequence in fragments shorter than    1063 bp
  15% of sequence in fragments shorter than    1383 bp
  20% of sequence in fragments shorter than    1672 bp
  25% of sequence in fragments shorter than    1955 bp
  30% of sequence in fragments shorter than    2232 bp
  35% of sequence in fragments shorter than    2524 bp
  40% of sequence in fragments shorter than    2809 bp
  45% of sequence in fragments shorter than    3115 bp
  50% of sequence in fragments shorter than    3440 bp
  55% of sequence in fragments shorter than    3773 bp
  60% of sequence in fragments shorter than    4151 bp
  65% of sequence in fragments shorter than    4569 bp
  70% of sequence in fragments shorter than    5041 bp
  75% of sequence in fragments shorter than    5588 bp
  80% of sequence in fragments shorter than    6254 bp
  85% of sequence in fragments shorter than    7101 bp
  90% of sequence in fragments shorter than    8310 bp
  95% of sequence in fragments shorter than   10505 bp

# PpuMI.SpeI (RGGWCCY+ACTAGT) double digest, 1485 thousand fragments (1085138+400286 sites), 2006 bp average fragment length
   5% of sequence in fragments shorter than     682 bp
  10% of sequence in fragments shorter than    1065 bp
  15% of sequence in fragments shorter than    1382 bp
  20% of sequence in fragments shorter than    1708 bp
  25% of sequence in fragments shorter than    2024 bp
  30% of sequence in fragments shorter than    2344 bp
  35% of sequence in fragments shorter than    2676 bp
  40% of sequence in fragments shorter than    3018 bp
  45% of sequence in fragments shorter than    3370 bp
  50% of sequence in fragments shorter than    3721 bp
  55% of sequence in fragments shorter than    4123 bp
  60% of sequence in fragments shorter than    4569 bp
  65% of sequence in fragments shorter than    5075 bp
  70% of sequence in fragments shorter than    5654 bp
  75% of sequence in fragments shorter than    6327 bp
  80% of sequence in fragments shorter than    7166 bp
  85% of sequence in fragments shorter than    8238 bp
  90% of sequence in fragments shorter than    9819 bp
  95% of sequence in fragments shorter than   12933 bp

# AflIII.MluI (ACRYGT+ACGCGT) double digest, 1484 thousand fragments (1485394+21415 sites), 2006 bp average fragment length
   5% of sequence in fragments shorter than     725 bp
  10% of sequence in fragments shorter than    1131 bp
  15% of sequence in fragments shorter than    1476 bp
  20% of sequence in fragments shorter than    1802 bp
  25% of sequence in fragments shorter than    2106 bp
  30% of sequence in fragments shorter than    2417 bp
  35% of sequence in fragments shorter than    2735 bp
  40% of sequence in fragments shorter than    3052 bp
  45% of sequence in fragments shorter than    3392 bp
  50% of sequence in fragments shorter than    3757 bp
  55% of sequence in fragments shorter than    4149 bp
  60% of sequence in fragments shorter than    4577 bp
  65% of sequence in fragments shorter than    5053 bp
  70% of sequence in fragments shorter than    5598 bp
  75% of sequence in fragments shorter than    6210 bp
  80% of sequence in fragments shorter than    6943 bp
  85% of sequence in fragments shorter than    7894 bp
  90% of sequence in fragments shorter than    9256 bp
  95% of sequence in fragments shorter than   11829 bp

# PvuII.SpeI (CAGCTG+ACTAGT) double digest, 1484 thousand fragments (1084260+400286 sites), 2007 bp average fragment length
   5% of sequence in fragments shorter than     700 bp
  10% of sequence in fragments shorter than    1064 bp
  15% of sequence in fragments shorter than    1382 bp
  20% of sequence in fragments shorter than    1690 bp
  25% of sequence in fragments shorter than    1987 bp
  30% of sequence in fragments shorter than    2281 bp
  35% of sequence in fragments shorter than    2589 bp
  40% of sequence in fragments shorter than    2899 bp
  45% of sequence in fragments shorter than    3230 bp
  50% of sequence in fragments shorter than    3584 bp
  55% of sequence in fragments shorter than    3958 bp
  60% of sequence in fragments shorter than    4379 bp
  65% of sequence in fragments shorter than    4810 bp
  70% of sequence in fragments shorter than    5338 bp
  75% of sequence in fragments shorter than    5956 bp
  80% of sequence in fragments shorter than    6701 bp
  85% of sequence in fragments shorter than    7659 bp
  90% of sequence in fragments shorter than    9053 bp
  95% of sequence in fragments shorter than   11729 bp

# AvaIII.SphI (ATGCAT+GCATGC) double digest, 1483 thousand fragments (933357+550952 sites), 2008 bp average fragment length
   5% of sequence in fragments shorter than     758 bp
  10% of sequence in fragments shorter than    1150 bp
  15% of sequence in fragments shorter than    1488 bp
  20% of sequence in fragments shorter than    1806 bp
  25% of sequence in fragments shorter than    2109 bp
  30% of sequence in fragments shorter than    2418 bp
  35% of sequence in fragments shorter than    2734 bp
  40% of sequence in fragments shorter than    3058 bp
  45% of sequence in fragments shorter than    3400 bp
  50% of sequence in fragments shorter than    3764 bp
  55% of sequence in fragments shorter than    4156 bp
  60% of sequence in fragments shorter than    4588 bp
  65% of sequence in fragments shorter than    5064 bp
  70% of sequence in fragments shorter than    5616 bp
  75% of sequence in fragments shorter than    6251 bp
  80% of sequence in fragments shorter than    7021 bp
  85% of sequence in fragments shorter than    8011 bp
  90% of sequence in fragments shorter than    9431 bp
  95% of sequence in fragments shorter than   12171 bp

# FauI.PmeI (CCCGC+GTTTAAAC) double digest, 1481 thousand fragments (1439772+42094 sites), 2011 bp average fragment length
   5% of sequence in fragments shorter than     696 bp
  10% of sequence in fragments shorter than    1232 bp
  15% of sequence in fragments shorter than    1729 bp
  20% of sequence in fragments shorter than    2231 bp
  25% of sequence in fragments shorter than    2760 bp
  30% of sequence in fragments shorter than    3313 bp
  35% of sequence in fragments shorter than    3911 bp
  40% of sequence in fragments shorter than    4515 bp
  45% of sequence in fragments shorter than    5206 bp
  50% of sequence in fragments shorter than    5961 bp
  55% of sequence in fragments shorter than    6797 bp
  60% of sequence in fragments shorter than    7722 bp
  65% of sequence in fragments shorter than    8768 bp
  70% of sequence in fragments shorter than   10011 bp
  75% of sequence in fragments shorter than   11459 bp
  80% of sequence in fragments shorter than   13235 bp
  85% of sequence in fragments shorter than   15615 bp
  90% of sequence in fragments shorter than   19100 bp
  95% of sequence in fragments shorter than   25660 bp

# HaeII.XbaI (RGCGCY+TCTAGA) double digest, 1480 thousand fragments (630101+850998 sites), 2012 bp average fragment length
   5% of sequence in fragments shorter than     686 bp
  10% of sequence in fragments shorter than    1063 bp
  15% of sequence in fragments shorter than    1417 bp
  20% of sequence in fragments shorter than    1757 bp
  25% of sequence in fragments shorter than    2087 bp
  30% of sequence in fragments shorter than    2398 bp
  35% of sequence in fragments shorter than    2735 bp
  40% of sequence in fragments shorter than    3069 bp
  45% of sequence in fragments shorter than    3428 bp
  50% of sequence in fragments shorter than    3808 bp
  55% of sequence in fragments shorter than    4212 bp
  60% of sequence in fragments shorter than    4649 bp
  65% of sequence in fragments shorter than    5133 bp
  70% of sequence in fragments shorter than    5669 bp
  75% of sequence in fragments shorter than    6299 bp
  80% of sequence in fragments shorter than    7042 bp
  85% of sequence in fragments shorter than    7986 bp
  90% of sequence in fragments shorter than    9309 bp
  95% of sequence in fragments shorter than   11509 bp

# PacI.PstI (TTAATTAA+CTGCAG) double digest, 1480 thousand fragments (159455+1321469 sites), 2012 bp average fragment length
   5% of sequence in fragments shorter than     681 bp
  10% of sequence in fragments shorter than    1053 bp
  15% of sequence in fragments shorter than    1383 bp
  20% of sequence in fragments shorter than    1711 bp
  25% of sequence in fragments shorter than    2029 bp
  30% of sequence in fragments shorter than    2346 bp
  35% of sequence in fragments shorter than    2681 bp
  40% of sequence in fragments shorter than    3026 bp
  45% of sequence in fragments shorter than    3395 bp
  50% of sequence in fragments shorter than    3788 bp
  55% of sequence in fragments shorter than    4213 bp
  60% of sequence in fragments shorter than    4684 bp
  65% of sequence in fragments shorter than    5197 bp
  70% of sequence in fragments shorter than    5774 bp
  75% of sequence in fragments shorter than    6450 bp
  80% of sequence in fragments shorter than    7287 bp
  85% of sequence in fragments shorter than    8341 bp
  90% of sequence in fragments shorter than    9891 bp
  95% of sequence in fragments shorter than   12727 bp

# BspMI.PmeI (ACCTGC+GTTTAAAC) double digest, 1479 thousand fragments (1438129+42094 sites), 2013 bp average fragment length
   5% of sequence in fragments shorter than     684 bp
  10% of sequence in fragments shorter than    1049 bp
  15% of sequence in fragments shorter than    1384 bp
  20% of sequence in fragments shorter than    1710 bp
  25% of sequence in fragments shorter than    2007 bp
  30% of sequence in fragments shorter than    2325 bp
  35% of sequence in fragments shorter than    2656 bp
  40% of sequence in fragments shorter than    2995 bp
  45% of sequence in fragments shorter than    3309 bp
  50% of sequence in fragments shorter than    3687 bp
  55% of sequence in fragments shorter than    4100 bp
  60% of sequence in fragments shorter than    4555 bp
  65% of sequence in fragments shorter than    5056 bp
  70% of sequence in fragments shorter than    5625 bp
  75% of sequence in fragments shorter than    6288 bp
  80% of sequence in fragments shorter than    7095 bp
  85% of sequence in fragments shorter than    8136 bp
  90% of sequence in fragments shorter than    9569 bp
  95% of sequence in fragments shorter than   12098 bp

# BceAI.BglII (ACGGC+AGATCT) double digest, 1479 thousand fragments (705178+774732 sites), 2014 bp average fragment length
   5% of sequence in fragments shorter than     694 bp
  10% of sequence in fragments shorter than    1068 bp
  15% of sequence in fragments shorter than    1398 bp
  20% of sequence in fragments shorter than    1698 bp
  25% of sequence in fragments shorter than    2002 bp
  30% of sequence in fragments shorter than    2308 bp
  35% of sequence in fragments shorter than    2622 bp
  40% of sequence in fragments shorter than    2947 bp
  45% of sequence in fragments shorter than    3289 bp
  50% of sequence in fragments shorter than    3653 bp
  55% of sequence in fragments shorter than    4042 bp
  60% of sequence in fragments shorter than    4462 bp
  65% of sequence in fragments shorter than    4929 bp
  70% of sequence in fragments shorter than    5467 bp
  75% of sequence in fragments shorter than    6080 bp
  80% of sequence in fragments shorter than    6827 bp
  85% of sequence in fragments shorter than    7799 bp
  90% of sequence in fragments shorter than    9162 bp
  95% of sequence in fragments shorter than   11741 bp

# Hpy99I.PstI (CGWCG+CTGCAG) double digest, 1478 thousand fragments (157917+1321469 sites), 2014 bp average fragment length
   5% of sequence in fragments shorter than     659 bp
  10% of sequence in fragments shorter than    1042 bp
  15% of sequence in fragments shorter than    1391 bp
  20% of sequence in fragments shorter than    1743 bp
  25% of sequence in fragments shorter than    2086 bp
  30% of sequence in fragments shorter than    2437 bp
  35% of sequence in fragments shorter than    2801 bp
  40% of sequence in fragments shorter than    3190 bp
  45% of sequence in fragments shorter than    3603 bp
  50% of sequence in fragments shorter than    4050 bp
  55% of sequence in fragments shorter than    4541 bp
  60% of sequence in fragments shorter than    5072 bp
  65% of sequence in fragments shorter than    5659 bp
  70% of sequence in fragments shorter than    6335 bp
  75% of sequence in fragments shorter than    7138 bp
  80% of sequence in fragments shorter than    8101 bp
  85% of sequence in fragments shorter than    9370 bp
  90% of sequence in fragments shorter than   11201 bp
  95% of sequence in fragments shorter than   14631 bp

# AccI.SapI (GTMKAC+GCTCTTC) double digest, 1477 thousand fragments (1101063+377161 sites), 2016 bp average fragment length
   5% of sequence in fragments shorter than     719 bp
  10% of sequence in fragments shorter than    1076 bp
  15% of sequence in fragments shorter than    1381 bp
  20% of sequence in fragments shorter than    1665 bp
  25% of sequence in fragments shorter than    1946 bp
  30% of sequence in fragments shorter than    2234 bp
  35% of sequence in fragments shorter than    2521 bp
  40% of sequence in fragments shorter than    2812 bp
  45% of sequence in fragments shorter than    3124 bp
  50% of sequence in fragments shorter than    3453 bp
  55% of sequence in fragments shorter than    3774 bp
  60% of sequence in fragments shorter than    4156 bp
  65% of sequence in fragments shorter than    4569 bp
  70% of sequence in fragments shorter than    5047 bp
  75% of sequence in fragments shorter than    5598 bp
  80% of sequence in fragments shorter than    6271 bp
  85% of sequence in fragments shorter than    7137 bp
  90% of sequence in fragments shorter than    8372 bp
  95% of sequence in fragments shorter than   10647 bp

# AccI.SmaI (GTMKAC+CCCGGG) double digest, 1477 thousand fragments (1101063+376940 sites), 2016 bp average fragment length
   5% of sequence in fragments shorter than     703 bp
  10% of sequence in fragments shorter than    1073 bp
  15% of sequence in fragments shorter than    1383 bp
  20% of sequence in fragments shorter than    1682 bp
  25% of sequence in fragments shorter than    1968 bp
  30% of sequence in fragments shorter than    2259 bp
  35% of sequence in fragments shorter than    2553 bp
  40% of sequence in fragments shorter than    2850 bp
  45% of sequence in fragments shorter than    3168 bp
  50% of sequence in fragments shorter than    3507 bp
  55% of sequence in fragments shorter than    3868 bp
  60% of sequence in fragments shorter than    4265 bp
  65% of sequence in fragments shorter than    4696 bp
  70% of sequence in fragments shorter than    5191 bp
  75% of sequence in fragments shorter than    5740 bp
  80% of sequence in fragments shorter than    6359 bp
  85% of sequence in fragments shorter than    7224 bp
  90% of sequence in fragments shorter than    8446 bp
  95% of sequence in fragments shorter than   10714 bp

# EcoRI.HaeII (GAATTC+RGCGCY) double digest, 1477 thousand fragments (847401+630101 sites), 2017 bp average fragment length
   5% of sequence in fragments shorter than     664 bp
  10% of sequence in fragments shorter than    1072 bp
  15% of sequence in fragments shorter than    1453 bp
  20% of sequence in fragments shorter than    1807 bp
  25% of sequence in fragments shorter than    2144 bp
  30% of sequence in fragments shorter than    2488 bp
  35% of sequence in fragments shorter than    2827 bp
  40% of sequence in fragments shorter than    3174 bp
  45% of sequence in fragments shorter than    3538 bp
  50% of sequence in fragments shorter than    3930 bp
  55% of sequence in fragments shorter than    4342 bp
  60% of sequence in fragments shorter than    4798 bp
  65% of sequence in fragments shorter than    5297 bp
  70% of sequence in fragments shorter than    5855 bp
  75% of sequence in fragments shorter than    6502 bp
  80% of sequence in fragments shorter than    7280 bp
  85% of sequence in fragments shorter than    8263 bp
  90% of sequence in fragments shorter than    9617 bp
  95% of sequence in fragments shorter than   11904 bp

# AvaIII.ScaI (ATGCAT+AGTACT) double digest, 1476 thousand fragments (933357+543793 sites), 2017 bp average fragment length
   5% of sequence in fragments shorter than     704 bp
  10% of sequence in fragments shorter than    1071 bp
  15% of sequence in fragments shorter than    1391 bp
  20% of sequence in fragments shorter than    1692 bp
  25% of sequence in fragments shorter than    1991 bp
  30% of sequence in fragments shorter than    2285 bp
  35% of sequence in fragments shorter than    2593 bp
  40% of sequence in fragments shorter than    2909 bp
  45% of sequence in fragments shorter than    3252 bp
  50% of sequence in fragments shorter than    3609 bp
  55% of sequence in fragments shorter than    4002 bp
  60% of sequence in fragments shorter than    4440 bp
  65% of sequence in fragments shorter than    4928 bp
  70% of sequence in fragments shorter than    5501 bp
  75% of sequence in fragments shorter than    6181 bp
  80% of sequence in fragments shorter than    7021 bp
  85% of sequence in fragments shorter than    8127 bp
  90% of sequence in fragments shorter than    9787 bp
  95% of sequence in fragments shorter than   13177 bp

# SphI.StuI (GCATGC+AGGCCT) double digest, 1476 thousand fragments (550952+925729 sites), 2018 bp average fragment length
   5% of sequence in fragments shorter than     667 bp
  10% of sequence in fragments shorter than    1022 bp
  15% of sequence in fragments shorter than    1372 bp
  20% of sequence in fragments shorter than    1701 bp
  25% of sequence in fragments shorter than    2014 bp
  30% of sequence in fragments shorter than    2350 bp
  35% of sequence in fragments shorter than    2695 bp
  40% of sequence in fragments shorter than    3048 bp
  45% of sequence in fragments shorter than    3421 bp
  50% of sequence in fragments shorter than    3816 bp
  55% of sequence in fragments shorter than    4243 bp
  60% of sequence in fragments shorter than    4712 bp
  65% of sequence in fragments shorter than    5230 bp
  70% of sequence in fragments shorter than    5818 bp
  75% of sequence in fragments shorter than    6495 bp
  80% of sequence in fragments shorter than    7319 bp
  85% of sequence in fragments shorter than    8351 bp
  90% of sequence in fragments shorter than    9827 bp
  95% of sequence in fragments shorter than   12362 bp

# HgaI.NdeI (GACGC+CATATG) double digest, 1474 thousand fragments (571889+903360 sites), 2020 bp average fragment length
   5% of sequence in fragments shorter than     706 bp
  10% of sequence in fragments shorter than    1084 bp
  15% of sequence in fragments shorter than    1399 bp
  20% of sequence in fragments shorter than    1718 bp
  25% of sequence in fragments shorter than    2030 bp
  30% of sequence in fragments shorter than    2346 bp
  35% of sequence in fragments shorter than    2665 bp
  40% of sequence in fragments shorter than    2987 bp
  45% of sequence in fragments shorter than    3325 bp
  50% of sequence in fragments shorter than    3687 bp
  55% of sequence in fragments shorter than    4072 bp
  60% of sequence in fragments shorter than    4487 bp
  65% of sequence in fragments shorter than    4944 bp
  70% of sequence in fragments shorter than    5468 bp
  75% of sequence in fragments shorter than    6077 bp
  80% of sequence in fragments shorter than    6808 bp
  85% of sequence in fragments shorter than    7752 bp
  90% of sequence in fragments shorter than    9112 bp
  95% of sequence in fragments shorter than   11654 bp

# EciI.NdeI (GGCGGA+CATATG) double digest, 1474 thousand fragments (571273+903360 sites), 2021 bp average fragment length
   5% of sequence in fragments shorter than     696 bp
  10% of sequence in fragments shorter than    1064 bp
  15% of sequence in fragments shorter than    1379 bp
  20% of sequence in fragments shorter than    1692 bp
  25% of sequence in fragments shorter than    2002 bp
  30% of sequence in fragments shorter than    2312 bp
  35% of sequence in fragments shorter than    2630 bp
  40% of sequence in fragments shorter than    2952 bp
  45% of sequence in fragments shorter than    3290 bp
  50% of sequence in fragments shorter than    3651 bp
  55% of sequence in fragments shorter than    4043 bp
  60% of sequence in fragments shorter than    4468 bp
  65% of sequence in fragments shorter than    4929 bp
  70% of sequence in fragments shorter than    5448 bp
  75% of sequence in fragments shorter than    6065 bp
  80% of sequence in fragments shorter than    6810 bp
  85% of sequence in fragments shorter than    7792 bp
  90% of sequence in fragments shorter than    9215 bp
  95% of sequence in fragments shorter than   11990 bp

# HpaI.PpuMI (GTTAAC+RGGWCCY) double digest, 1474 thousand fragments (389418+1085138 sites), 2021 bp average fragment length
   5% of sequence in fragments shorter than     680 bp
  10% of sequence in fragments shorter than    1068 bp
  15% of sequence in fragments shorter than    1411 bp
  20% of sequence in fragments shorter than    1735 bp
  25% of sequence in fragments shorter than    2037 bp
  30% of sequence in fragments shorter than    2362 bp
  35% of sequence in fragments shorter than    2699 bp
  40% of sequence in fragments shorter than    3053 bp
  45% of sequence in fragments shorter than    3421 bp
  50% of sequence in fragments shorter than    3790 bp
  55% of sequence in fragments shorter than    4206 bp
  60% of sequence in fragments shorter than    4666 bp
  65% of sequence in fragments shorter than    5189 bp
  70% of sequence in fragments shorter than    5783 bp
  75% of sequence in fragments shorter than    6485 bp
  80% of sequence in fragments shorter than    7339 bp
  85% of sequence in fragments shorter than    8455 bp
  90% of sequence in fragments shorter than   10093 bp
  95% of sequence in fragments shorter than   13441 bp

# ApaLI.BspHI (GTGCAC+TCATGA) double digest, 1474 thousand fragments (496255+978290 sites), 2021 bp average fragment length
   5% of sequence in fragments shorter than     721 bp
  10% of sequence in fragments shorter than    1072 bp
  15% of sequence in fragments shorter than    1386 bp
  20% of sequence in fragments shorter than    1677 bp
  25% of sequence in fragments shorter than    1944 bp
  30% of sequence in fragments shorter than    2222 bp
  35% of sequence in fragments shorter than    2510 bp
  40% of sequence in fragments shorter than    2810 bp
  45% of sequence in fragments shorter than    3119 bp
  50% of sequence in fragments shorter than    3451 bp
  55% of sequence in fragments shorter than    3810 bp
  60% of sequence in fragments shorter than    4185 bp
  65% of sequence in fragments shorter than    4601 bp
  70% of sequence in fragments shorter than    5076 bp
  75% of sequence in fragments shorter than    5634 bp
  80% of sequence in fragments shorter than    6313 bp
  85% of sequence in fragments shorter than    7163 bp
  90% of sequence in fragments shorter than    8412 bp
  95% of sequence in fragments shorter than   10770 bp

# HpaI.PvuII (GTTAAC+CAGCTG) double digest, 1473 thousand fragments (389418+1084260 sites), 2022 bp average fragment length
   5% of sequence in fragments shorter than     696 bp
  10% of sequence in fragments shorter than    1064 bp
  15% of sequence in fragments shorter than    1389 bp
  20% of sequence in fragments shorter than    1700 bp
  25% of sequence in fragments shorter than    2005 bp
  30% of sequence in fragments shorter than    2314 bp
  35% of sequence in fragments shorter than    2631 bp
  40% of sequence in fragments shorter than    2949 bp
  45% of sequence in fragments shorter than    3299 bp
  50% of sequence in fragments shorter than    3671 bp
  55% of sequence in fragments shorter than    4072 bp
  60% of sequence in fragments shorter than    4508 bp
  65% of sequence in fragments shorter than    5003 bp
  70% of sequence in fragments shorter than    5557 bp
  75% of sequence in fragments shorter than    6201 bp
  80% of sequence in fragments shorter than    6988 bp
  85% of sequence in fragments shorter than    8014 bp
  90% of sequence in fragments shorter than    9499 bp
  95% of sequence in fragments shorter than   12486 bp

# BsaAI.NcoI (YACGTR+CCATGG) double digest, 1472 thousand fragments (713801+759594 sites), 2023 bp average fragment length
   5% of sequence in fragments shorter than     712 bp
  10% of sequence in fragments shorter than    1077 bp
  15% of sequence in fragments shorter than    1413 bp
  20% of sequence in fragments shorter than    1730 bp
  25% of sequence in fragments shorter than    2033 bp
  30% of sequence in fragments shorter than    2340 bp
  35% of sequence in fragments shorter than    2653 bp
  40% of sequence in fragments shorter than    2976 bp
  45% of sequence in fragments shorter than    3314 bp
  50% of sequence in fragments shorter than    3654 bp
  55% of sequence in fragments shorter than    4047 bp
  60% of sequence in fragments shorter than    4460 bp
  65% of sequence in fragments shorter than    4920 bp
  70% of sequence in fragments shorter than    5449 bp
  75% of sequence in fragments shorter than    6057 bp
  80% of sequence in fragments shorter than    6806 bp
  85% of sequence in fragments shorter than    7780 bp
  90% of sequence in fragments shorter than    9194 bp
  95% of sequence in fragments shorter than   11717 bp

# BclI.FnuDII (TGATCA+CGCG) double digest, 1472 thousand fragments (738785+733940 sites), 2024 bp average fragment length
   5% of sequence in fragments shorter than     787 bp
  10% of sequence in fragments shorter than    1248 bp
  15% of sequence in fragments shorter than    1656 bp
  20% of sequence in fragments shorter than    2032 bp
  25% of sequence in fragments shorter than    2407 bp
  30% of sequence in fragments shorter than    2769 bp
  35% of sequence in fragments shorter than    3146 bp
  40% of sequence in fragments shorter than    3536 bp
  45% of sequence in fragments shorter than    3937 bp
  50% of sequence in fragments shorter than    4370 bp
  55% of sequence in fragments shorter than    4842 bp
  60% of sequence in fragments shorter than    5353 bp
  65% of sequence in fragments shorter than    5925 bp
  70% of sequence in fragments shorter than    6576 bp
  75% of sequence in fragments shorter than    7346 bp
  80% of sequence in fragments shorter than    8284 bp
  85% of sequence in fragments shorter than    9499 bp
  90% of sequence in fragments shorter than   11279 bp
  95% of sequence in fragments shorter than   14689 bp

# FauI.SalI (CCCGC+GTCGAC) double digest, 1470 thousand fragments (1439772+31396 sites), 2026 bp average fragment length
   5% of sequence in fragments shorter than     698 bp
  10% of sequence in fragments shorter than    1242 bp
  15% of sequence in fragments shorter than    1751 bp
  20% of sequence in fragments shorter than    2270 bp
  25% of sequence in fragments shorter than    2814 bp
  30% of sequence in fragments shorter than    3391 bp
  35% of sequence in fragments shorter than    4014 bp
  40% of sequence in fragments shorter than    4645 bp
  45% of sequence in fragments shorter than    5366 bp
  50% of sequence in fragments shorter than    6158 bp
  55% of sequence in fragments shorter than    7035 bp
  60% of sequence in fragments shorter than    8018 bp
  65% of sequence in fragments shorter than    9122 bp
  70% of sequence in fragments shorter than   10418 bp
  75% of sequence in fragments shorter than   11959 bp
  80% of sequence in fragments shorter than   13857 bp
  85% of sequence in fragments shorter than   16412 bp
  90% of sequence in fragments shorter than   20114 bp
  95% of sequence in fragments shorter than   27162 bp

# BciVI.BglII (GTATCC+AGATCT) double digest, 1470 thousand fragments (696093+774732 sites), 2026 bp average fragment length
   5% of sequence in fragments shorter than     714 bp
  10% of sequence in fragments shorter than    1074 bp
  15% of sequence in fragments shorter than    1377 bp
  20% of sequence in fragments shorter than    1660 bp
  25% of sequence in fragments shorter than    1936 bp
  30% of sequence in fragments shorter than    2215 bp
  35% of sequence in fragments shorter than    2505 bp
  40% of sequence in fragments shorter than    2804 bp
  45% of sequence in fragments shorter than    3121 bp
  50% of sequence in fragments shorter than    3458 bp
  55% of sequence in fragments shorter than    3817 bp
  60% of sequence in fragments shorter than    4214 bp
  65% of sequence in fragments shorter than    4653 bp
  70% of sequence in fragments shorter than    5151 bp
  75% of sequence in fragments shorter than    5734 bp
  80% of sequence in fragments shorter than    6442 bp
  85% of sequence in fragments shorter than    7355 bp
  90% of sequence in fragments shorter than    8715 bp
  95% of sequence in fragments shorter than   11256 bp

# BspMI.SalI (ACCTGC+GTCGAC) double digest, 1469 thousand fragments (1438129+31396 sites), 2028 bp average fragment length
   5% of sequence in fragments shorter than     685 bp
  10% of sequence in fragments shorter than    1056 bp
  15% of sequence in fragments shorter than    1392 bp
  20% of sequence in fragments shorter than    1725 bp
  25% of sequence in fragments shorter than    2026 bp
  30% of sequence in fragments shorter than    2352 bp
  35% of sequence in fragments shorter than    2688 bp
  40% of sequence in fragments shorter than    3033 bp
  45% of sequence in fragments shorter than    3352 bp
  50% of sequence in fragments shorter than    3742 bp
  55% of sequence in fragments shorter than    4165 bp
  60% of sequence in fragments shorter than    4629 bp
  65% of sequence in fragments shorter than    5146 bp
  70% of sequence in fragments shorter than    5728 bp
  75% of sequence in fragments shorter than    6413 bp
  80% of sequence in fragments shorter than    7244 bp
  85% of sequence in fragments shorter than    8316 bp
  90% of sequence in fragments shorter than    9806 bp
  95% of sequence in fragments shorter than   12432 bp

# ScaI.StuI (AGTACT+AGGCCT) double digest, 1469 thousand fragments (543793+925729 sites), 2028 bp average fragment length
   5% of sequence in fragments shorter than     685 bp
  10% of sequence in fragments shorter than    1041 bp
  15% of sequence in fragments shorter than    1384 bp
  20% of sequence in fragments shorter than    1706 bp
  25% of sequence in fragments shorter than    2008 bp
  30% of sequence in fragments shorter than    2332 bp
  35% of sequence in fragments shorter than    2658 bp
  40% of sequence in fragments shorter than    2988 bp
  45% of sequence in fragments shorter than    3333 bp
  50% of sequence in fragments shorter than    3698 bp
  55% of sequence in fragments shorter than    4089 bp
  60% of sequence in fragments shorter than    4514 bp
  65% of sequence in fragments shorter than    4981 bp
  70% of sequence in fragments shorter than    5506 bp
  75% of sequence in fragments shorter than    6106 bp
  80% of sequence in fragments shorter than    6840 bp
  85% of sequence in fragments shorter than    7747 bp
  90% of sequence in fragments shorter than    9000 bp
  95% of sequence in fragments shorter than   11133 bp

# MfeI.NdeI (CAATTG+CATATG) double digest, 1467 thousand fragments (564303+903360 sites), 2031 bp average fragment length
   5% of sequence in fragments shorter than     681 bp
  10% of sequence in fragments shorter than    1047 bp
  15% of sequence in fragments shorter than    1367 bp
  20% of sequence in fragments shorter than    1684 bp
  25% of sequence in fragments shorter than    1997 bp
  30% of sequence in fragments shorter than    2312 bp
  35% of sequence in fragments shorter than    2640 bp
  40% of sequence in fragments shorter than    2981 bp
  45% of sequence in fragments shorter than    3344 bp
  50% of sequence in fragments shorter than    3738 bp
  55% of sequence in fragments shorter than    4157 bp
  60% of sequence in fragments shorter than    4615 bp
  65% of sequence in fragments shorter than    5125 bp
  70% of sequence in fragments shorter than    5725 bp
  75% of sequence in fragments shorter than    6432 bp
  80% of sequence in fragments shorter than    7338 bp
  85% of sequence in fragments shorter than    8564 bp
  90% of sequence in fragments shorter than   10494 bp
  95% of sequence in fragments shorter than   14711 bp

# AccI.BamHI (GTMKAC+GGATCC) double digest, 1466 thousand fragments (1101063+365999 sites), 2031 bp average fragment length
   5% of sequence in fragments shorter than     725 bp
  10% of sequence in fragments shorter than    1096 bp
  15% of sequence in fragments shorter than    1384 bp
  20% of sequence in fragments shorter than    1680 bp
  25% of sequence in fragments shorter than    1966 bp
  30% of sequence in fragments shorter than    2250 bp
  35% of sequence in fragments shorter than    2544 bp
  40% of sequence in fragments shorter than    2842 bp
  45% of sequence in fragments shorter than    3160 bp
  50% of sequence in fragments shorter than    3492 bp
  55% of sequence in fragments shorter than    3853 bp
  60% of sequence in fragments shorter than    4237 bp
  65% of sequence in fragments shorter than    4651 bp
  70% of sequence in fragments shorter than    5094 bp
  75% of sequence in fragments shorter than    5644 bp
  80% of sequence in fragments shorter than    6310 bp
  85% of sequence in fragments shorter than    7157 bp
  90% of sequence in fragments shorter than    8365 bp
  95% of sequence in fragments shorter than   10510 bp

# BscGI.BsrBI (CCCGT+CCGCTC) double digest, 1466 thousand fragments (1231070+235523 sites), 2032 bp average fragment length
   5% of sequence in fragments shorter than     573 bp
  10% of sequence in fragments shorter than    1026 bp
  15% of sequence in fragments shorter than    1454 bp
  20% of sequence in fragments shorter than    1885 bp
  25% of sequence in fragments shorter than    2326 bp
  30% of sequence in fragments shorter than    2782 bp
  35% of sequence in fragments shorter than    3262 bp
  40% of sequence in fragments shorter than    3770 bp
  45% of sequence in fragments shorter than    4314 bp
  50% of sequence in fragments shorter than    4900 bp
  55% of sequence in fragments shorter than    5550 bp
  60% of sequence in fragments shorter than    6264 bp
  65% of sequence in fragments shorter than    7067 bp
  70% of sequence in fragments shorter than    7997 bp
  75% of sequence in fragments shorter than    9099 bp
  80% of sequence in fragments shorter than   10434 bp
  85% of sequence in fragments shorter than   12178 bp
  90% of sequence in fragments shorter than   14637 bp
  95% of sequence in fragments shorter than   18816 bp

# ApaI.AvaI (GGGCCC+CYCGRG) double digest, 1465 thousand fragments (460339+1005714 sites), 2033 bp average fragment length
   5% of sequence in fragments shorter than     643 bp
  10% of sequence in fragments shorter than    1082 bp
  15% of sequence in fragments shorter than    1506 bp
  20% of sequence in fragments shorter than    1937 bp
  25% of sequence in fragments shorter than    2390 bp
  30% of sequence in fragments shorter than    2869 bp
  35% of sequence in fragments shorter than    3386 bp
  40% of sequence in fragments shorter than    3943 bp
  45% of sequence in fragments shorter than    4544 bp
  50% of sequence in fragments shorter than    5205 bp
  55% of sequence in fragments shorter than    5934 bp
  60% of sequence in fragments shorter than    6766 bp
  65% of sequence in fragments shorter than    7710 bp
  70% of sequence in fragments shorter than    8806 bp
  75% of sequence in fragments shorter than   10121 bp
  80% of sequence in fragments shorter than   11781 bp
  85% of sequence in fragments shorter than   13989 bp
  90% of sequence in fragments shorter than   17327 bp
  95% of sequence in fragments shorter than   24390 bp

# BceAI.NcoI (ACGGC+CCATGG) double digest, 1464 thousand fragments (705178+759594 sites), 2035 bp average fragment length
   5% of sequence in fragments shorter than     676 bp
  10% of sequence in fragments shorter than    1073 bp
  15% of sequence in fragments shorter than    1422 bp
  20% of sequence in fragments shorter than    1769 bp
  25% of sequence in fragments shorter than    2111 bp
  30% of sequence in fragments shorter than    2460 bp
  35% of sequence in fragments shorter than    2818 bp
  40% of sequence in fragments shorter than    3195 bp
  45% of sequence in fragments shorter than    3589 bp
  50% of sequence in fragments shorter than    4013 bp
  55% of sequence in fragments shorter than    4468 bp
  60% of sequence in fragments shorter than    4947 bp
  65% of sequence in fragments shorter than    5466 bp
  70% of sequence in fragments shorter than    6080 bp
  75% of sequence in fragments shorter than    6822 bp
  80% of sequence in fragments shorter than    7737 bp
  85% of sequence in fragments shorter than    8907 bp
  90% of sequence in fragments shorter than   10606 bp
  95% of sequence in fragments shorter than   13728 bp

# HindIII.SacI (AAGCTT+GAGCTC) double digest, 1463 thousand fragments (860365+603323 sites), 2036 bp average fragment length
   5% of sequence in fragments shorter than     710 bp
  10% of sequence in fragments shorter than    1090 bp
  15% of sequence in fragments shorter than    1407 bp
  20% of sequence in fragments shorter than    1716 bp
  25% of sequence in fragments shorter than    1983 bp
  30% of sequence in fragments shorter than    2276 bp
  35% of sequence in fragments shorter than    2576 bp
  40% of sequence in fragments shorter than    2883 bp
  45% of sequence in fragments shorter than    3199 bp
  50% of sequence in fragments shorter than    3536 bp
  55% of sequence in fragments shorter than    3898 bp
  60% of sequence in fragments shorter than    4289 bp
  65% of sequence in fragments shorter than    4723 bp
  70% of sequence in fragments shorter than    5210 bp
  75% of sequence in fragments shorter than    5787 bp
  80% of sequence in fragments shorter than    6482 bp
  85% of sequence in fragments shorter than    7371 bp
  90% of sequence in fragments shorter than    8629 bp
  95% of sequence in fragments shorter than   10948 bp

# PpuMI.SapI (RGGWCCY+GCTCTTC) double digest, 1461 thousand fragments (1085138+377161 sites), 2038 bp average fragment length
   5% of sequence in fragments shorter than     671 bp
  10% of sequence in fragments shorter than    1050 bp
  15% of sequence in fragments shorter than    1417 bp
  20% of sequence in fragments shorter than    1772 bp
  25% of sequence in fragments shorter than    2095 bp
  30% of sequence in fragments shorter than    2455 bp
  35% of sequence in fragments shorter than    2824 bp
  40% of sequence in fragments shorter than    3214 bp
  45% of sequence in fragments shorter than    3632 bp
  50% of sequence in fragments shorter than    4077 bp
  55% of sequence in fragments shorter than    4561 bp
  60% of sequence in fragments shorter than    5102 bp
  65% of sequence in fragments shorter than    5710 bp
  70% of sequence in fragments shorter than    6403 bp
  75% of sequence in fragments shorter than    7230 bp
  80% of sequence in fragments shorter than    8244 bp
  85% of sequence in fragments shorter than    9604 bp
  90% of sequence in fragments shorter than   11588 bp
  95% of sequence in fragments shorter than   15697 bp

# PpuMI.SmaI (RGGWCCY+CCCGGG) double digest, 1461 thousand fragments (1085138+376940 sites), 2038 bp average fragment length
   5% of sequence in fragments shorter than     654 bp
  10% of sequence in fragments shorter than    1064 bp
  15% of sequence in fragments shorter than    1446 bp
  20% of sequence in fragments shorter than    1820 bp
  25% of sequence in fragments shorter than    2191 bp
  30% of sequence in fragments shorter than    2579 bp
  35% of sequence in fragments shorter than    2981 bp
  40% of sequence in fragments shorter than    3393 bp
  45% of sequence in fragments shorter than    3801 bp
  50% of sequence in fragments shorter than    4276 bp
  55% of sequence in fragments shorter than    4809 bp
  60% of sequence in fragments shorter than    5405 bp
  65% of sequence in fragments shorter than    6081 bp
  70% of sequence in fragments shorter than    6855 bp
  75% of sequence in fragments shorter than    7779 bp
  80% of sequence in fragments shorter than    8916 bp
  85% of sequence in fragments shorter than   10432 bp
  90% of sequence in fragments shorter than   12723 bp
  95% of sequence in fragments shorter than   17429 bp

# PvuII.SapI (CAGCTG+GCTCTTC) double digest, 1461 thousand fragments (1084260+377161 sites), 2039 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1059 bp
  15% of sequence in fragments shorter than    1399 bp
  20% of sequence in fragments shorter than    1731 bp
  25% of sequence in fragments shorter than    2065 bp
  30% of sequence in fragments shorter than    2400 bp
  35% of sequence in fragments shorter than    2747 bp
  40% of sequence in fragments shorter than    3103 bp
  45% of sequence in fragments shorter than    3489 bp
  50% of sequence in fragments shorter than    3903 bp
  55% of sequence in fragments shorter than    4348 bp
  60% of sequence in fragments shorter than    4833 bp
  65% of sequence in fragments shorter than    5379 bp
  70% of sequence in fragments shorter than    6001 bp
  75% of sequence in fragments shorter than    6744 bp
  80% of sequence in fragments shorter than    7646 bp
  85% of sequence in fragments shorter than    8844 bp
  90% of sequence in fragments shorter than   10627 bp
  95% of sequence in fragments shorter than   14116 bp

# BscGI.KasI (CCCGT+GGCGCC) double digest, 1460 thousand fragments (1231070+230381 sites), 2039 bp average fragment length
   5% of sequence in fragments shorter than     588 bp
  10% of sequence in fragments shorter than    1048 bp
  15% of sequence in fragments shorter than    1496 bp
  20% of sequence in fragments shorter than    1942 bp
  25% of sequence in fragments shorter than    2398 bp
  30% of sequence in fragments shorter than    2867 bp
  35% of sequence in fragments shorter than    3366 bp
  40% of sequence in fragments shorter than    3892 bp
  45% of sequence in fragments shorter than    4458 bp
  50% of sequence in fragments shorter than    5074 bp
  55% of sequence in fragments shorter than    5749 bp
  60% of sequence in fragments shorter than    6493 bp
  65% of sequence in fragments shorter than    7337 bp
  70% of sequence in fragments shorter than    8318 bp
  75% of sequence in fragments shorter than    9495 bp
  80% of sequence in fragments shorter than   10902 bp
  85% of sequence in fragments shorter than   12743 bp
  90% of sequence in fragments shorter than   15323 bp
  95% of sequence in fragments shorter than   19751 bp

# PvuII.SmaI (CAGCTG+CCCGGG) double digest, 1460 thousand fragments (1084260+376940 sites), 2039 bp average fragment length
   5% of sequence in fragments shorter than     666 bp
  10% of sequence in fragments shorter than    1052 bp
  15% of sequence in fragments shorter than    1401 bp
  20% of sequence in fragments shorter than    1748 bp
  25% of sequence in fragments shorter than    2098 bp
  30% of sequence in fragments shorter than    2453 bp
  35% of sequence in fragments shorter than    2819 bp
  40% of sequence in fragments shorter than    3206 bp
  45% of sequence in fragments shorter than    3628 bp
  50% of sequence in fragments shorter than    4089 bp
  55% of sequence in fragments shorter than    4591 bp
  60% of sequence in fragments shorter than    5153 bp
  65% of sequence in fragments shorter than    5781 bp
  70% of sequence in fragments shorter than    6497 bp
  75% of sequence in fragments shorter than    7334 bp
  80% of sequence in fragments shorter than    8384 bp
  85% of sequence in fragments shorter than    9776 bp
  90% of sequence in fragments shorter than   11868 bp
  95% of sequence in fragments shorter than   16011 bp

# FauI.MluI (CCCGC+ACGCGT) double digest, 1460 thousand fragments (1439772+21415 sites), 2039 bp average fragment length
   5% of sequence in fragments shorter than     702 bp
  10% of sequence in fragments shorter than    1254 bp
  15% of sequence in fragments shorter than    1771 bp
  20% of sequence in fragments shorter than    2299 bp
  25% of sequence in fragments shorter than    2857 bp
  30% of sequence in fragments shorter than    3448 bp
  35% of sequence in fragments shorter than    4085 bp
  40% of sequence in fragments shorter than    4734 bp
  45% of sequence in fragments shorter than    5475 bp
  50% of sequence in fragments shorter than    6295 bp
  55% of sequence in fragments shorter than    7193 bp
  60% of sequence in fragments shorter than    8212 bp
  65% of sequence in fragments shorter than    9364 bp
  70% of sequence in fragments shorter than   10700 bp
  75% of sequence in fragments shorter than   12295 bp
  80% of sequence in fragments shorter than   14257 bp
  85% of sequence in fragments shorter than   16895 bp
  90% of sequence in fragments shorter than   20764 bp
  95% of sequence in fragments shorter than   28146 bp

# BspMI.MluI (ACCTGC+ACGCGT) double digest, 1459 thousand fragments (1438129+21415 sites), 2042 bp average fragment length
   5% of sequence in fragments shorter than     688 bp
  10% of sequence in fragments shorter than    1063 bp
  15% of sequence in fragments shorter than    1402 bp
  20% of sequence in fragments shorter than    1737 bp
  25% of sequence in fragments shorter than    2041 bp
  30% of sequence in fragments shorter than    2371 bp
  35% of sequence in fragments shorter than    2711 bp
  40% of sequence in fragments shorter than    3060 bp
  45% of sequence in fragments shorter than    3382 bp
  50% of sequence in fragments shorter than    3777 bp
  55% of sequence in fragments shorter than    4204 bp
  60% of sequence in fragments shorter than    4673 bp
  65% of sequence in fragments shorter than    5197 bp
  70% of sequence in fragments shorter than    5784 bp
  75% of sequence in fragments shorter than    6472 bp
  80% of sequence in fragments shorter than    7316 bp
  85% of sequence in fragments shorter than    8405 bp
  90% of sequence in fragments shorter than    9911 bp
  95% of sequence in fragments shorter than   12592 bp

# BscGI.SwaI (CCCGT+ATTTAAAT) double digest, 1457 thousand fragments (1231070+226602 sites), 2045 bp average fragment length
   5% of sequence in fragments shorter than     615 bp
  10% of sequence in fragments shorter than    1035 bp
  15% of sequence in fragments shorter than    1430 bp
  20% of sequence in fragments shorter than    1811 bp
  25% of sequence in fragments shorter than    2188 bp
  30% of sequence in fragments shorter than    2571 bp
  35% of sequence in fragments shorter than    2963 bp
  40% of sequence in fragments shorter than    3373 bp
  45% of sequence in fragments shorter than    3804 bp
  50% of sequence in fragments shorter than    4268 bp
  55% of sequence in fragments shorter than    4768 bp
  60% of sequence in fragments shorter than    5316 bp
  65% of sequence in fragments shorter than    5922 bp
  70% of sequence in fragments shorter than    6612 bp
  75% of sequence in fragments shorter than    7414 bp
  80% of sequence in fragments shorter than    8389 bp
  85% of sequence in fragments shorter than    9636 bp
  90% of sequence in fragments shorter than   11380 bp
  95% of sequence in fragments shorter than   14337 bp

# BciVI.NcoI (GTATCC+CCATGG) double digest, 1455 thousand fragments (696093+759594 sites), 2047 bp average fragment length
   5% of sequence in fragments shorter than     731 bp
  10% of sequence in fragments shorter than    1112 bp
  15% of sequence in fragments shorter than    1414 bp
  20% of sequence in fragments shorter than    1709 bp
  25% of sequence in fragments shorter than    2000 bp
  30% of sequence in fragments shorter than    2294 bp
  35% of sequence in fragments shorter than    2598 bp
  40% of sequence in fragments shorter than    2916 bp
  45% of sequence in fragments shorter than    3236 bp
  50% of sequence in fragments shorter than    3583 bp
  55% of sequence in fragments shorter than    3955 bp
  60% of sequence in fragments shorter than    4354 bp
  65% of sequence in fragments shorter than    4802 bp
  70% of sequence in fragments shorter than    5312 bp
  75% of sequence in fragments shorter than    5911 bp
  80% of sequence in fragments shorter than    6640 bp
  85% of sequence in fragments shorter than    7573 bp
  90% of sequence in fragments shorter than    8924 bp
  95% of sequence in fragments shorter than   11448 bp

# AvrII.HindIII (CCTAGG+AAGCTT) double digest, 1454 thousand fragments (594956+860365 sites), 2048 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1081 bp
  15% of sequence in fragments shorter than    1394 bp
  20% of sequence in fragments shorter than    1697 bp
  25% of sequence in fragments shorter than    1961 bp
  30% of sequence in fragments shorter than    2250 bp
  35% of sequence in fragments shorter than    2543 bp
  40% of sequence in fragments shorter than    2841 bp
  45% of sequence in fragments shorter than    3153 bp
  50% of sequence in fragments shorter than    3484 bp
  55% of sequence in fragments shorter than    3840 bp
  60% of sequence in fragments shorter than    4228 bp
  65% of sequence in fragments shorter than    4655 bp
  70% of sequence in fragments shorter than    5138 bp
  75% of sequence in fragments shorter than    5705 bp
  80% of sequence in fragments shorter than    6394 bp
  85% of sequence in fragments shorter than    7283 bp
  90% of sequence in fragments shorter than    8563 bp
  95% of sequence in fragments shorter than   10925 bp

# FauI.SgrAI (CCCGC+CRCCGGYG) double digest, 1454 thousand fragments (1439772+15270 sites), 2048 bp average fragment length
   5% of sequence in fragments shorter than     709 bp
  10% of sequence in fragments shorter than    1266 bp
  15% of sequence in fragments shorter than    1788 bp
  20% of sequence in fragments shorter than    2321 bp
  25% of sequence in fragments shorter than    2886 bp
  30% of sequence in fragments shorter than    3482 bp
  35% of sequence in fragments shorter than    4123 bp
  40% of sequence in fragments shorter than    4778 bp
  45% of sequence in fragments shorter than    5529 bp
  50% of sequence in fragments shorter than    6359 bp
  55% of sequence in fragments shorter than    7266 bp
  60% of sequence in fragments shorter than    8287 bp
  65% of sequence in fragments shorter than    9459 bp
  70% of sequence in fragments shorter than   10812 bp
  75% of sequence in fragments shorter than   12429 bp
  80% of sequence in fragments shorter than   14422 bp
  85% of sequence in fragments shorter than   17088 bp
  90% of sequence in fragments shorter than   20998 bp
  95% of sequence in fragments shorter than   28426 bp

# FauI.NruI (CCCGC+TCGCGA) double digest, 1454 thousand fragments (1439772+14872 sites), 2049 bp average fragment length
   5% of sequence in fragments shorter than     708 bp
  10% of sequence in fragments shorter than    1263 bp
  15% of sequence in fragments shorter than    1782 bp
  20% of sequence in fragments shorter than    2313 bp
  25% of sequence in fragments shorter than    2874 bp
  30% of sequence in fragments shorter than    3468 bp
  35% of sequence in fragments shorter than    4108 bp
  40% of sequence in fragments shorter than    4759 bp
  45% of sequence in fragments shorter than    5507 bp
  50% of sequence in fragments shorter than    6332 bp
  55% of sequence in fragments shorter than    7238 bp
  60% of sequence in fragments shorter than    8258 bp
  65% of sequence in fragments shorter than    9420 bp
  70% of sequence in fragments shorter than   10770 bp
  75% of sequence in fragments shorter than   12377 bp
  80% of sequence in fragments shorter than   14341 bp
  85% of sequence in fragments shorter than   16999 bp
  90% of sequence in fragments shorter than   20874 bp
  95% of sequence in fragments shorter than   28325 bp

# NdeI.SphI (CATATG+GCATGC) double digest, 1453 thousand fragments (903360+550952 sites), 2049 bp average fragment length
   5% of sequence in fragments shorter than     719 bp
  10% of sequence in fragments shorter than    1083 bp
  15% of sequence in fragments shorter than    1393 bp
  20% of sequence in fragments shorter than    1699 bp
  25% of sequence in fragments shorter than    1997 bp
  30% of sequence in fragments shorter than    2298 bp
  35% of sequence in fragments shorter than    2603 bp
  40% of sequence in fragments shorter than    2917 bp
  45% of sequence in fragments shorter than    3248 bp
  50% of sequence in fragments shorter than    3596 bp
  55% of sequence in fragments shorter than    3970 bp
  60% of sequence in fragments shorter than    4376 bp
  65% of sequence in fragments shorter than    4827 bp
  70% of sequence in fragments shorter than    5332 bp
  75% of sequence in fragments shorter than    5919 bp
  80% of sequence in fragments shorter than    6637 bp
  85% of sequence in fragments shorter than    7566 bp
  90% of sequence in fragments shorter than    8926 bp
  95% of sequence in fragments shorter than   11614 bp

# SacI.XbaI (GAGCTC+TCTAGA) double digest, 1453 thousand fragments (603323+850998 sites), 2049 bp average fragment length
   5% of sequence in fragments shorter than     727 bp
  10% of sequence in fragments shorter than    1081 bp
  15% of sequence in fragments shorter than    1388 bp
  20% of sequence in fragments shorter than    1674 bp
  25% of sequence in fragments shorter than    1961 bp
  30% of sequence in fragments shorter than    2245 bp
  35% of sequence in fragments shorter than    2540 bp
  40% of sequence in fragments shorter than    2848 bp
  45% of sequence in fragments shorter than    3169 bp
  50% of sequence in fragments shorter than    3510 bp
  55% of sequence in fragments shorter than    3873 bp
  60% of sequence in fragments shorter than    4270 bp
  65% of sequence in fragments shorter than    4706 bp
  70% of sequence in fragments shorter than    5196 bp
  75% of sequence in fragments shorter than    5768 bp
  80% of sequence in fragments shorter than    6462 bp
  85% of sequence in fragments shorter than    7335 bp
  90% of sequence in fragments shorter than    8554 bp
  95% of sequence in fragments shorter than   10721 bp

# FauI.PvuI (CCCGC+CGATCG) double digest, 1453 thousand fragments (1439772+14186 sites), 2050 bp average fragment length
   5% of sequence in fragments shorter than     708 bp
  10% of sequence in fragments shorter than    1263 bp
  15% of sequence in fragments shorter than    1780 bp
  20% of sequence in fragments shorter than    2311 bp
  25% of sequence in fragments shorter than    2873 bp
  30% of sequence in fragments shorter than    3465 bp
  35% of sequence in fragments shorter than    4104 bp
  40% of sequence in fragments shorter than    4757 bp
  45% of sequence in fragments shorter than    5504 bp
  50% of sequence in fragments shorter than    6326 bp
  55% of sequence in fragments shorter than    7230 bp
  60% of sequence in fragments shorter than    8247 bp
  65% of sequence in fragments shorter than    9407 bp
  70% of sequence in fragments shorter than   10753 bp
  75% of sequence in fragments shorter than   12352 bp
  80% of sequence in fragments shorter than   14317 bp
  85% of sequence in fragments shorter than   16967 bp
  90% of sequence in fragments shorter than   20833 bp
  95% of sequence in fragments shorter than   28242 bp

# PstI.SnaBI (CTGCAG+TACGTA) double digest, 1453 thousand fragments (1321469+132299 sites), 2050 bp average fragment length
   5% of sequence in fragments shorter than     685 bp
  10% of sequence in fragments shorter than    1067 bp
  15% of sequence in fragments shorter than    1399 bp
  20% of sequence in fragments shorter than    1733 bp
  25% of sequence in fragments shorter than    2061 bp
  30% of sequence in fragments shorter than    2386 bp
  35% of sequence in fragments shorter than    2730 bp
  40% of sequence in fragments shorter than    3079 bp
  45% of sequence in fragments shorter than    3457 bp
  50% of sequence in fragments shorter than    3866 bp
  55% of sequence in fragments shorter than    4305 bp
  60% of sequence in fragments shorter than    4792 bp
  65% of sequence in fragments shorter than    5325 bp
  70% of sequence in fragments shorter than    5929 bp
  75% of sequence in fragments shorter than    6642 bp
  80% of sequence in fragments shorter than    7512 bp
  85% of sequence in fragments shorter than    8624 bp
  90% of sequence in fragments shorter than   10223 bp
  95% of sequence in fragments shorter than   13104 bp

# FauI.FseI (CCCGC+GGCCGGCC) double digest, 1453 thousand fragments (1439772+13662 sites), 2050 bp average fragment length
   5% of sequence in fragments shorter than     709 bp
  10% of sequence in fragments shorter than    1266 bp
  15% of sequence in fragments shorter than    1788 bp
  20% of sequence in fragments shorter than    2323 bp
  25% of sequence in fragments shorter than    2888 bp
  30% of sequence in fragments shorter than    3486 bp
  35% of sequence in fragments shorter than    4128 bp
  40% of sequence in fragments shorter than    4785 bp
  45% of sequence in fragments shorter than    5538 bp
  50% of sequence in fragments shorter than    6371 bp
  55% of sequence in fragments shorter than    7282 bp
  60% of sequence in fragments shorter than    8310 bp
  65% of sequence in fragments shorter than    9484 bp
  70% of sequence in fragments shorter than   10843 bp
  75% of sequence in fragments shorter than   12461 bp
  80% of sequence in fragments shorter than   14451 bp
  85% of sequence in fragments shorter than   17140 bp
  90% of sequence in fragments shorter than   21055 bp
  95% of sequence in fragments shorter than   28566 bp

# BspMI.SgrAI (ACCTGC+CRCCGGYG) double digest, 1452 thousand fragments (1438129+15270 sites), 2050 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1069 bp
  15% of sequence in fragments shorter than    1410 bp
  20% of sequence in fragments shorter than    1746 bp
  25% of sequence in fragments shorter than    2053 bp
  30% of sequence in fragments shorter than    2384 bp
  35% of sequence in fragments shorter than    2726 bp
  40% of sequence in fragments shorter than    3077 bp
  45% of sequence in fragments shorter than    3401 bp
  50% of sequence in fragments shorter than    3798 bp
  55% of sequence in fragments shorter than    4228 bp
  60% of sequence in fragments shorter than    4700 bp
  65% of sequence in fragments shorter than    5228 bp
  70% of sequence in fragments shorter than    5820 bp
  75% of sequence in fragments shorter than    6513 bp
  80% of sequence in fragments shorter than    7359 bp
  85% of sequence in fragments shorter than    8455 bp
  90% of sequence in fragments shorter than    9968 bp
  95% of sequence in fragments shorter than   12658 bp

# BspMI.NruI (ACCTGC+TCGCGA) double digest, 1452 thousand fragments (1438129+14872 sites), 2051 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1069 bp
  15% of sequence in fragments shorter than    1409 bp
  20% of sequence in fragments shorter than    1744 bp
  25% of sequence in fragments shorter than    2049 bp
  30% of sequence in fragments shorter than    2378 bp
  35% of sequence in fragments shorter than    2719 bp
  40% of sequence in fragments shorter than    3069 bp
  45% of sequence in fragments shorter than    3392 bp
  50% of sequence in fragments shorter than    3788 bp
  55% of sequence in fragments shorter than    4217 bp
  60% of sequence in fragments shorter than    4686 bp
  65% of sequence in fragments shorter than    5210 bp
  70% of sequence in fragments shorter than    5802 bp
  75% of sequence in fragments shorter than    6491 bp
  80% of sequence in fragments shorter than    7335 bp
  85% of sequence in fragments shorter than    8426 bp
  90% of sequence in fragments shorter than    9933 bp
  95% of sequence in fragments shorter than   12617 bp

# BclI.BsaAI (TGATCA+YACGTR) double digest, 1452 thousand fragments (738785+713801 sites), 2052 bp average fragment length
   5% of sequence in fragments shorter than     743 bp
  10% of sequence in fragments shorter than    1099 bp
  15% of sequence in fragments shorter than    1420 bp
  20% of sequence in fragments shorter than    1711 bp
  25% of sequence in fragments shorter than    1980 bp
  30% of sequence in fragments shorter than    2270 bp
  35% of sequence in fragments shorter than    2559 bp
  40% of sequence in fragments shorter than    2857 bp
  45% of sequence in fragments shorter than    3171 bp
  50% of sequence in fragments shorter than    3501 bp
  55% of sequence in fragments shorter than    3862 bp
  60% of sequence in fragments shorter than    4250 bp
  65% of sequence in fragments shorter than    4676 bp
  70% of sequence in fragments shorter than    5161 bp
  75% of sequence in fragments shorter than    5719 bp
  80% of sequence in fragments shorter than    6396 bp
  85% of sequence in fragments shorter than    7262 bp
  90% of sequence in fragments shorter than    8479 bp
  95% of sequence in fragments shorter than   10703 bp

# BspMI.PvuI (ACCTGC+CGATCG) double digest, 1451 thousand fragments (1438129+14186 sites), 2052 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1070 bp
  15% of sequence in fragments shorter than    1411 bp
  20% of sequence in fragments shorter than    1747 bp
  25% of sequence in fragments shorter than    2053 bp
  30% of sequence in fragments shorter than    2382 bp
  35% of sequence in fragments shorter than    2724 bp
  40% of sequence in fragments shorter than    3074 bp
  45% of sequence in fragments shorter than    3397 bp
  50% of sequence in fragments shorter than    3794 bp
  55% of sequence in fragments shorter than    4224 bp
  60% of sequence in fragments shorter than    4694 bp
  65% of sequence in fragments shorter than    5219 bp
  70% of sequence in fragments shorter than    5810 bp
  75% of sequence in fragments shorter than    6499 bp
  80% of sequence in fragments shorter than    7345 bp
  85% of sequence in fragments shorter than    8437 bp
  90% of sequence in fragments shorter than    9936 bp
  95% of sequence in fragments shorter than   12609 bp

# AvaI.EcoRV (CYCGRG+GATATC) double digest, 1451 thousand fragments (1005714+446473 sites), 2052 bp average fragment length
   5% of sequence in fragments shorter than     698 bp
  10% of sequence in fragments shorter than    1091 bp
  15% of sequence in fragments shorter than    1457 bp
  20% of sequence in fragments shorter than    1795 bp
  25% of sequence in fragments shorter than    2142 bp
  30% of sequence in fragments shorter than    2488 bp
  35% of sequence in fragments shorter than    2853 bp
  40% of sequence in fragments shorter than    3235 bp
  45% of sequence in fragments shorter than    3641 bp
  50% of sequence in fragments shorter than    4072 bp
  55% of sequence in fragments shorter than    4539 bp
  60% of sequence in fragments shorter than    5050 bp
  65% of sequence in fragments shorter than    5620 bp
  70% of sequence in fragments shorter than    6278 bp
  75% of sequence in fragments shorter than    7044 bp
  80% of sequence in fragments shorter than    7987 bp
  85% of sequence in fragments shorter than    9214 bp
  90% of sequence in fragments shorter than   10976 bp
  95% of sequence in fragments shorter than   14253 bp

# BspMI.FseI (ACCTGC+GGCCGGCC) double digest, 1451 thousand fragments (1438129+13662 sites), 2053 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1070 bp
  15% of sequence in fragments shorter than    1411 bp
  20% of sequence in fragments shorter than    1748 bp
  25% of sequence in fragments shorter than    2055 bp
  30% of sequence in fragments shorter than    2386 bp
  35% of sequence in fragments shorter than    2728 bp
  40% of sequence in fragments shorter than    3080 bp
  45% of sequence in fragments shorter than    3404 bp
  50% of sequence in fragments shorter than    3803 bp
  55% of sequence in fragments shorter than    4234 bp
  60% of sequence in fragments shorter than    4706 bp
  65% of sequence in fragments shorter than    5234 bp
  70% of sequence in fragments shorter than    5827 bp
  75% of sequence in fragments shorter than    6520 bp
  80% of sequence in fragments shorter than    7370 bp
  85% of sequence in fragments shorter than    8465 bp
  90% of sequence in fragments shorter than    9978 bp
  95% of sequence in fragments shorter than   12679 bp

# BamHI.PpuMI (GGATCC+RGGWCCY) double digest, 1450 thousand fragments (365999+1085138 sites), 2054 bp average fragment length
   5% of sequence in fragments shorter than     654 bp
  10% of sequence in fragments shorter than    1068 bp
  15% of sequence in fragments shorter than    1446 bp
  20% of sequence in fragments shorter than    1812 bp
  25% of sequence in fragments shorter than    2165 bp
  30% of sequence in fragments shorter than    2537 bp
  35% of sequence in fragments shorter than    2923 bp
  40% of sequence in fragments shorter than    3316 bp
  45% of sequence in fragments shorter than    3711 bp
  50% of sequence in fragments shorter than    4155 bp
  55% of sequence in fragments shorter than    4651 bp
  60% of sequence in fragments shorter than    5212 bp
  65% of sequence in fragments shorter than    5847 bp
  70% of sequence in fragments shorter than    6567 bp
  75% of sequence in fragments shorter than    7431 bp
  80% of sequence in fragments shorter than    8486 bp
  85% of sequence in fragments shorter than    9882 bp
  90% of sequence in fragments shorter than   11906 bp
  95% of sequence in fragments shorter than   15920 bp

# EcoRI.SacI (GAATTC+GAGCTC) double digest, 1450 thousand fragments (847401+603323 sites), 2054 bp average fragment length
   5% of sequence in fragments shorter than     692 bp
  10% of sequence in fragments shorter than    1082 bp
  15% of sequence in fragments shorter than    1411 bp
  20% of sequence in fragments shorter than    1710 bp
  25% of sequence in fragments shorter than    2008 bp
  30% of sequence in fragments shorter than    2312 bp
  35% of sequence in fragments shorter than    2618 bp
  40% of sequence in fragments shorter than    2930 bp
  45% of sequence in fragments shorter than    3260 bp
  50% of sequence in fragments shorter than    3605 bp
  55% of sequence in fragments shorter than    3980 bp
  60% of sequence in fragments shorter than    4379 bp
  65% of sequence in fragments shorter than    4821 bp
  70% of sequence in fragments shorter than    5322 bp
  75% of sequence in fragments shorter than    5891 bp
  80% of sequence in fragments shorter than    6580 bp
  85% of sequence in fragments shorter than    7454 bp
  90% of sequence in fragments shorter than    8671 bp
  95% of sequence in fragments shorter than   10767 bp

# BamHI.PvuII (GGATCC+CAGCTG) double digest, 1449 thousand fragments (365999+1084260 sites), 2055 bp average fragment length
   5% of sequence in fragments shorter than     695 bp
  10% of sequence in fragments shorter than    1084 bp
  15% of sequence in fragments shorter than    1430 bp
  20% of sequence in fragments shorter than    1758 bp
  25% of sequence in fragments shorter than    2087 bp
  30% of sequence in fragments shorter than    2417 bp
  35% of sequence in fragments shorter than    2756 bp
  40% of sequence in fragments shorter than    3112 bp
  45% of sequence in fragments shorter than    3489 bp
  50% of sequence in fragments shorter than    3905 bp
  55% of sequence in fragments shorter than    4317 bp
  60% of sequence in fragments shorter than    4810 bp
  65% of sequence in fragments shorter than    5368 bp
  70% of sequence in fragments shorter than    6007 bp
  75% of sequence in fragments shorter than    6758 bp
  80% of sequence in fragments shorter than    7671 bp
  85% of sequence in fragments shorter than    8866 bp
  90% of sequence in fragments shorter than   10640 bp
  95% of sequence in fragments shorter than   14016 bp

# FauI.NotI (CCCGC+GCGGCCGC) double digest, 1449 thousand fragments (1439772+9795 sites), 2056 bp average fragment length
   5% of sequence in fragments shorter than     712 bp
  10% of sequence in fragments shorter than    1272 bp
  15% of sequence in fragments shorter than    1797 bp
  20% of sequence in fragments shorter than    2332 bp
  25% of sequence in fragments shorter than    2900 bp
  30% of sequence in fragments shorter than    3500 bp
  35% of sequence in fragments shorter than    4143 bp
  40% of sequence in fragments shorter than    4802 bp
  45% of sequence in fragments shorter than    5556 bp
  50% of sequence in fragments shorter than    6392 bp
  55% of sequence in fragments shorter than    7306 bp
  60% of sequence in fragments shorter than    8335 bp
  65% of sequence in fragments shorter than    9508 bp
  70% of sequence in fragments shorter than   10874 bp
  75% of sequence in fragments shorter than   12496 bp
  80% of sequence in fragments shorter than   14491 bp
  85% of sequence in fragments shorter than   17180 bp
  90% of sequence in fragments shorter than   21120 bp
  95% of sequence in fragments shorter than   28620 bp

# FauI.RsrII (CCCGC+CGGWCCG) double digest, 1449 thousand fragments (1439772+9764 sites), 2056 bp average fragment length
   5% of sequence in fragments shorter than     711 bp
  10% of sequence in fragments shorter than    1269 bp
  15% of sequence in fragments shorter than    1792 bp
  20% of sequence in fragments shorter than    2327 bp
  25% of sequence in fragments shorter than    2893 bp
  30% of sequence in fragments shorter than    3491 bp
  35% of sequence in fragments shorter than    4133 bp
  40% of sequence in fragments shorter than    4790 bp
  45% of sequence in fragments shorter than    5542 bp
  50% of sequence in fragments shorter than    6374 bp
  55% of sequence in fragments shorter than    7285 bp
  60% of sequence in fragments shorter than    8313 bp
  65% of sequence in fragments shorter than    9486 bp
  70% of sequence in fragments shorter than   10848 bp
  75% of sequence in fragments shorter than   12464 bp
  80% of sequence in fragments shorter than   14463 bp
  85% of sequence in fragments shorter than   17146 bp
  90% of sequence in fragments shorter than   21051 bp
  95% of sequence in fragments shorter than   28561 bp

# BspMI.NotI (ACCTGC+GCGGCCGC) double digest, 1447 thousand fragments (1438129+9795 sites), 2058 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1073 bp
  15% of sequence in fragments shorter than    1415 bp
  20% of sequence in fragments shorter than    1753 bp
  25% of sequence in fragments shorter than    2060 bp
  30% of sequence in fragments shorter than    2392 bp
  35% of sequence in fragments shorter than    2735 bp
  40% of sequence in fragments shorter than    3087 bp
  45% of sequence in fragments shorter than    3412 bp
  50% of sequence in fragments shorter than    3810 bp
  55% of sequence in fragments shorter than    4243 bp
  60% of sequence in fragments shorter than    4715 bp
  65% of sequence in fragments shorter than    5243 bp
  70% of sequence in fragments shorter than    5837 bp
  75% of sequence in fragments shorter than    6533 bp
  80% of sequence in fragments shorter than    7381 bp
  85% of sequence in fragments shorter than    8479 bp
  90% of sequence in fragments shorter than    9994 bp
  95% of sequence in fragments shorter than   12692 bp

# BspMI.RsrII (ACCTGC+CGGWCCG) double digest, 1447 thousand fragments (1438129+9764 sites), 2058 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1073 bp
  15% of sequence in fragments shorter than    1415 bp
  20% of sequence in fragments shorter than    1752 bp
  25% of sequence in fragments shorter than    2059 bp
  30% of sequence in fragments shorter than    2390 bp
  35% of sequence in fragments shorter than    2733 bp
  40% of sequence in fragments shorter than    3085 bp
  45% of sequence in fragments shorter than    3410 bp
  50% of sequence in fragments shorter than    3807 bp
  55% of sequence in fragments shorter than    4238 bp
  60% of sequence in fragments shorter than    4710 bp
  65% of sequence in fragments shorter than    5238 bp
  70% of sequence in fragments shorter than    5831 bp
  75% of sequence in fragments shorter than    6525 bp
  80% of sequence in fragments shorter than    7373 bp
  85% of sequence in fragments shorter than    8468 bp
  90% of sequence in fragments shorter than    9983 bp
  95% of sequence in fragments shorter than   12680 bp

# BsaAI.FnuDII (YACGTR+CGCG) double digest, 1447 thousand fragments (713801+733940 sites), 2059 bp average fragment length
   5% of sequence in fragments shorter than     789 bp
  10% of sequence in fragments shorter than    1286 bp
  15% of sequence in fragments shorter than    1732 bp
  20% of sequence in fragments shorter than    2157 bp
  25% of sequence in fragments shorter than    2588 bp
  30% of sequence in fragments shorter than    3014 bp
  35% of sequence in fragments shorter than    3458 bp
  40% of sequence in fragments shorter than    3902 bp
  45% of sequence in fragments shorter than    4394 bp
  50% of sequence in fragments shorter than    4909 bp
  55% of sequence in fragments shorter than    5480 bp
  60% of sequence in fragments shorter than    6109 bp
  65% of sequence in fragments shorter than    6810 bp
  70% of sequence in fragments shorter than    7622 bp
  75% of sequence in fragments shorter than    8557 bp
  80% of sequence in fragments shorter than    9721 bp
  85% of sequence in fragments shorter than   11218 bp
  90% of sequence in fragments shorter than   13367 bp
  95% of sequence in fragments shorter than   17195 bp

# NaeI.PstI (GCCGGC+CTGCAG) double digest, 1446 thousand fragments (125692+1321469 sites), 2059 bp average fragment length
   5% of sequence in fragments shorter than     672 bp
  10% of sequence in fragments shorter than    1067 bp
  15% of sequence in fragments shorter than    1425 bp
  20% of sequence in fragments shorter than    1783 bp
  25% of sequence in fragments shorter than    2128 bp
  30% of sequence in fragments shorter than    2496 bp
  35% of sequence in fragments shorter than    2875 bp
  40% of sequence in fragments shorter than    3274 bp
  45% of sequence in fragments shorter than    3700 bp
  50% of sequence in fragments shorter than    4158 bp
  55% of sequence in fragments shorter than    4664 bp
  60% of sequence in fragments shorter than    5209 bp
  65% of sequence in fragments shorter than    5817 bp
  70% of sequence in fragments shorter than    6510 bp
  75% of sequence in fragments shorter than    7338 bp
  80% of sequence in fragments shorter than    8345 bp
  85% of sequence in fragments shorter than    9671 bp
  90% of sequence in fragments shorter than   11576 bp
  95% of sequence in fragments shorter than   15197 bp

# NdeI.ScaI (CATATG+AGTACT) double digest, 1446 thousand fragments (903360+543793 sites), 2059 bp average fragment length
   5% of sequence in fragments shorter than     705 bp
  10% of sequence in fragments shorter than    1069 bp
  15% of sequence in fragments shorter than    1383 bp
  20% of sequence in fragments shorter than    1696 bp
  25% of sequence in fragments shorter than    2006 bp
  30% of sequence in fragments shorter than    2324 bp
  35% of sequence in fragments shorter than    2646 bp
  40% of sequence in fragments shorter than    2982 bp
  45% of sequence in fragments shorter than    3342 bp
  50% of sequence in fragments shorter than    3729 bp
  55% of sequence in fragments shorter than    4144 bp
  60% of sequence in fragments shorter than    4600 bp
  65% of sequence in fragments shorter than    5102 bp
  70% of sequence in fragments shorter than    5685 bp
  75% of sequence in fragments shorter than    6380 bp
  80% of sequence in fragments shorter than    7274 bp
  85% of sequence in fragments shorter than    8464 bp
  90% of sequence in fragments shorter than   10341 bp
  95% of sequence in fragments shorter than   14588 bp

# AvrII.XbaI (CCTAGG+TCTAGA) double digest, 1445 thousand fragments (594956+850998 sites), 2061 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1049 bp
  15% of sequence in fragments shorter than    1384 bp
  20% of sequence in fragments shorter than    1702 bp
  25% of sequence in fragments shorter than    2000 bp
  30% of sequence in fragments shorter than    2304 bp
  35% of sequence in fragments shorter than    2613 bp
  40% of sequence in fragments shorter than    2931 bp
  45% of sequence in fragments shorter than    3257 bp
  50% of sequence in fragments shorter than    3600 bp
  55% of sequence in fragments shorter than    3968 bp
  60% of sequence in fragments shorter than    4369 bp
  65% of sequence in fragments shorter than    4808 bp
  70% of sequence in fragments shorter than    5301 bp
  75% of sequence in fragments shorter than    5878 bp
  80% of sequence in fragments shorter than    6579 bp
  85% of sequence in fragments shorter than    7461 bp
  90% of sequence in fragments shorter than    8695 bp
  95% of sequence in fragments shorter than   10903 bp

# AscI.FauI (GGCGCGCC+CCCGC) double digest, 1444 thousand fragments (4751+1439772 sites), 2063 bp average fragment length
   5% of sequence in fragments shorter than     714 bp
  10% of sequence in fragments shorter than    1274 bp
  15% of sequence in fragments shorter than    1798 bp
  20% of sequence in fragments shorter than    2334 bp
  25% of sequence in fragments shorter than    2901 bp
  30% of sequence in fragments shorter than    3502 bp
  35% of sequence in fragments shorter than    4144 bp
  40% of sequence in fragments shorter than    4804 bp
  45% of sequence in fragments shorter than    5558 bp
  50% of sequence in fragments shorter than    6393 bp
  55% of sequence in fragments shorter than    7307 bp
  60% of sequence in fragments shorter than    8336 bp
  65% of sequence in fragments shorter than    9510 bp
  70% of sequence in fragments shorter than   10875 bp
  75% of sequence in fragments shorter than   12499 bp
  80% of sequence in fragments shorter than   14497 bp
  85% of sequence in fragments shorter than   17183 bp
  90% of sequence in fragments shorter than   21131 bp
  95% of sequence in fragments shorter than   28641 bp

# BceAI.BclI (ACGGC+TGATCA) double digest, 1443 thousand fragments (705178+738785 sites), 2064 bp average fragment length
   5% of sequence in fragments shorter than     719 bp
  10% of sequence in fragments shorter than    1098 bp
  15% of sequence in fragments shorter than    1436 bp
  20% of sequence in fragments shorter than    1749 bp
  25% of sequence in fragments shorter than    2063 bp
  30% of sequence in fragments shorter than    2378 bp
  35% of sequence in fragments shorter than    2689 bp
  40% of sequence in fragments shorter than    3012 bp
  45% of sequence in fragments shorter than    3352 bp
  50% of sequence in fragments shorter than    3717 bp
  55% of sequence in fragments shorter than    4107 bp
  60% of sequence in fragments shorter than    4537 bp
  65% of sequence in fragments shorter than    5009 bp
  70% of sequence in fragments shorter than    5546 bp
  75% of sequence in fragments shorter than    6176 bp
  80% of sequence in fragments shorter than    6939 bp
  85% of sequence in fragments shorter than    7924 bp
  90% of sequence in fragments shorter than    9333 bp
  95% of sequence in fragments shorter than   12016 bp

# AscI.BspMI (GGCGCGCC+ACCTGC) double digest, 1442 thousand fragments (4751+1438129 sites), 2065 bp average fragment length
   5% of sequence in fragments shorter than     692 bp
  10% of sequence in fragments shorter than    1077 bp
  15% of sequence in fragments shorter than    1420 bp
  20% of sequence in fragments shorter than    1758 bp
  25% of sequence in fragments shorter than    2065 bp
  30% of sequence in fragments shorter than    2397 bp
  35% of sequence in fragments shorter than    2741 bp
  40% of sequence in fragments shorter than    3094 bp
  45% of sequence in fragments shorter than    3418 bp
  50% of sequence in fragments shorter than    3816 bp
  55% of sequence in fragments shorter than    4249 bp
  60% of sequence in fragments shorter than    4721 bp
  65% of sequence in fragments shorter than    5250 bp
  70% of sequence in fragments shorter than    5846 bp
  75% of sequence in fragments shorter than    6541 bp
  80% of sequence in fragments shorter than    7390 bp
  85% of sequence in fragments shorter than    8490 bp
  90% of sequence in fragments shorter than   10005 bp
  95% of sequence in fragments shorter than   12703 bp

# PstI.XhoI (CTGCAG+CTCGAG) double digest, 1442 thousand fragments (1321469+121308 sites), 2066 bp average fragment length
   5% of sequence in fragments shorter than     682 bp
  10% of sequence in fragments shorter than    1068 bp
  15% of sequence in fragments shorter than    1416 bp
  20% of sequence in fragments shorter than    1764 bp
  25% of sequence in fragments shorter than    2098 bp
  30% of sequence in fragments shorter than    2451 bp
  35% of sequence in fragments shorter than    2814 bp
  40% of sequence in fragments shorter than    3197 bp
  45% of sequence in fragments shorter than    3604 bp
  50% of sequence in fragments shorter than    4046 bp
  55% of sequence in fragments shorter than    4533 bp
  60% of sequence in fragments shorter than    5055 bp
  65% of sequence in fragments shorter than    5638 bp
  70% of sequence in fragments shorter than    6304 bp
  75% of sequence in fragments shorter than    7090 bp
  80% of sequence in fragments shorter than    8054 bp
  85% of sequence in fragments shorter than    9329 bp
  90% of sequence in fragments shorter than   11165 bp
  95% of sequence in fragments shorter than   14566 bp

# AvrII.EcoRI (CCTAGG+GAATTC) double digest, 1441 thousand fragments (594956+847401 sites), 2066 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1071 bp
  15% of sequence in fragments shorter than    1411 bp
  20% of sequence in fragments shorter than    1726 bp
  25% of sequence in fragments shorter than    2033 bp
  30% of sequence in fragments shorter than    2337 bp
  35% of sequence in fragments shorter than    2643 bp
  40% of sequence in fragments shorter than    2956 bp
  45% of sequence in fragments shorter than    3284 bp
  50% of sequence in fragments shorter than    3623 bp
  55% of sequence in fragments shorter than    3992 bp
  60% of sequence in fragments shorter than    4388 bp
  65% of sequence in fragments shorter than    4832 bp
  70% of sequence in fragments shorter than    5328 bp
  75% of sequence in fragments shorter than    5893 bp
  80% of sequence in fragments shorter than    6587 bp
  85% of sequence in fragments shorter than    7477 bp
  90% of sequence in fragments shorter than    8707 bp
  95% of sequence in fragments shorter than   10899 bp

# BceAI.FnuDII (ACGGC+CGCG) double digest, 1438 thousand fragments (705178+733940 sites), 2071 bp average fragment length
   5% of sequence in fragments shorter than     757 bp
  10% of sequence in fragments shorter than    1325 bp
  15% of sequence in fragments shorter than    1858 bp
  20% of sequence in fragments shorter than    2382 bp
  25% of sequence in fragments shorter than    2921 bp
  30% of sequence in fragments shorter than    3475 bp
  35% of sequence in fragments shorter than    4069 bp
  40% of sequence in fragments shorter than    4692 bp
  45% of sequence in fragments shorter than    5368 bp
  50% of sequence in fragments shorter than    6089 bp
  55% of sequence in fragments shorter than    6887 bp
  60% of sequence in fragments shorter than    7786 bp
  65% of sequence in fragments shorter than    8802 bp
  70% of sequence in fragments shorter than    9971 bp
  75% of sequence in fragments shorter than   11373 bp
  80% of sequence in fragments shorter than   13103 bp
  85% of sequence in fragments shorter than   15337 bp
  90% of sequence in fragments shorter than   18560 bp
  95% of sequence in fragments shorter than   24621 bp

# ApaI.BspHI (GGGCCC+TCATGA) double digest, 1438 thousand fragments (460339+978290 sites), 2071 bp average fragment length
   5% of sequence in fragments shorter than     721 bp
  10% of sequence in fragments shorter than    1099 bp
  15% of sequence in fragments shorter than    1436 bp
  20% of sequence in fragments shorter than    1757 bp
  25% of sequence in fragments shorter than    2066 bp
  30% of sequence in fragments shorter than    2364 bp
  35% of sequence in fragments shorter than    2678 bp
  40% of sequence in fragments shorter than    3004 bp
  45% of sequence in fragments shorter than    3343 bp
  50% of sequence in fragments shorter than    3705 bp
  55% of sequence in fragments shorter than    4095 bp
  60% of sequence in fragments shorter than    4516 bp
  65% of sequence in fragments shorter than    4983 bp
  70% of sequence in fragments shorter than    5513 bp
  75% of sequence in fragments shorter than    6136 bp
  80% of sequence in fragments shorter than    6900 bp
  85% of sequence in fragments shorter than    7885 bp
  90% of sequence in fragments shorter than    9327 bp
  95% of sequence in fragments shorter than   12132 bp

# AccI.BstEII (GTMKAC+GGTNACC) double digest, 1436 thousand fragments (1101063+335865 sites), 2074 bp average fragment length
   5% of sequence in fragments shorter than     737 bp
  10% of sequence in fragments shorter than    1112 bp
  15% of sequence in fragments shorter than    1425 bp
  20% of sequence in fragments shorter than    1725 bp
  25% of sequence in fragments shorter than    2013 bp
  30% of sequence in fragments shorter than    2308 bp
  35% of sequence in fragments shorter than    2607 bp
  40% of sequence in fragments shorter than    2911 bp
  45% of sequence in fragments shorter than    3232 bp
  50% of sequence in fragments shorter than    3569 bp
  55% of sequence in fragments shorter than    3931 bp
  60% of sequence in fragments shorter than    4323 bp
  65% of sequence in fragments shorter than    4701 bp
  70% of sequence in fragments shorter than    5184 bp
  75% of sequence in fragments shorter than    5746 bp
  80% of sequence in fragments shorter than    6423 bp
  85% of sequence in fragments shorter than    7297 bp
  90% of sequence in fragments shorter than    8554 bp
  95% of sequence in fragments shorter than   10858 bp

# BciVI.BclI (GTATCC+TGATCA) double digest, 1434 thousand fragments (696093+738785 sites), 2077 bp average fragment length
   5% of sequence in fragments shorter than     725 bp
  10% of sequence in fragments shorter than    1095 bp
  15% of sequence in fragments shorter than    1410 bp
  20% of sequence in fragments shorter than    1717 bp
  25% of sequence in fragments shorter than    2009 bp
  30% of sequence in fragments shorter than    2301 bp
  35% of sequence in fragments shorter than    2598 bp
  40% of sequence in fragments shorter than    2907 bp
  45% of sequence in fragments shorter than    3225 bp
  50% of sequence in fragments shorter than    3569 bp
  55% of sequence in fragments shorter than    3937 bp
  60% of sequence in fragments shorter than    4338 bp
  65% of sequence in fragments shorter than    4783 bp
  70% of sequence in fragments shorter than    5284 bp
  75% of sequence in fragments shorter than    5879 bp
  80% of sequence in fragments shorter than    6602 bp
  85% of sequence in fragments shorter than    7518 bp
  90% of sequence in fragments shorter than    8869 bp
  95% of sequence in fragments shorter than   11444 bp

# HgaI.HindIII (GACGC+AAGCTT) double digest, 1431 thousand fragments (571889+860365 sites), 2081 bp average fragment length
   5% of sequence in fragments shorter than     713 bp
  10% of sequence in fragments shorter than    1115 bp
  15% of sequence in fragments shorter than    1452 bp
  20% of sequence in fragments shorter than    1780 bp
  25% of sequence in fragments shorter than    2082 bp
  30% of sequence in fragments shorter than    2409 bp
  35% of sequence in fragments shorter than    2735 bp
  40% of sequence in fragments shorter than    3067 bp
  45% of sequence in fragments shorter than    3422 bp
  50% of sequence in fragments shorter than    3793 bp
  55% of sequence in fragments shorter than    4190 bp
  60% of sequence in fragments shorter than    4622 bp
  65% of sequence in fragments shorter than    5100 bp
  70% of sequence in fragments shorter than    5643 bp
  75% of sequence in fragments shorter than    6274 bp
  80% of sequence in fragments shorter than    7035 bp
  85% of sequence in fragments shorter than    8015 bp
  90% of sequence in fragments shorter than    9403 bp
  95% of sequence in fragments shorter than   11922 bp

# EciI.HindIII (GGCGGA+AAGCTT) double digest, 1431 thousand fragments (571273+860365 sites), 2082 bp average fragment length
   5% of sequence in fragments shorter than     712 bp
  10% of sequence in fragments shorter than    1105 bp
  15% of sequence in fragments shorter than    1435 bp
  20% of sequence in fragments shorter than    1756 bp
  25% of sequence in fragments shorter than    2039 bp
  30% of sequence in fragments shorter than    2350 bp
  35% of sequence in fragments shorter than    2664 bp
  40% of sequence in fragments shorter than    2986 bp
  45% of sequence in fragments shorter than    3323 bp
  50% of sequence in fragments shorter than    3688 bp
  55% of sequence in fragments shorter than    4075 bp
  60% of sequence in fragments shorter than    4498 bp
  65% of sequence in fragments shorter than    4968 bp
  70% of sequence in fragments shorter than    5503 bp
  75% of sequence in fragments shorter than    6122 bp
  80% of sequence in fragments shorter than    6884 bp
  85% of sequence in fragments shorter than    7859 bp
  90% of sequence in fragments shorter than    9251 bp
  95% of sequence in fragments shorter than   11848 bp

# BciVI.FnuDII (GTATCC+CGCG) double digest, 1429 thousand fragments (696093+733940 sites), 2084 bp average fragment length
   5% of sequence in fragments shorter than     816 bp
  10% of sequence in fragments shorter than    1315 bp
  15% of sequence in fragments shorter than    1731 bp
  20% of sequence in fragments shorter than    2117 bp
  25% of sequence in fragments shorter than    2496 bp
  30% of sequence in fragments shorter than    2888 bp
  35% of sequence in fragments shorter than    3286 bp
  40% of sequence in fragments shorter than    3699 bp
  45% of sequence in fragments shorter than    4130 bp
  50% of sequence in fragments shorter than    4585 bp
  55% of sequence in fragments shorter than    5074 bp
  60% of sequence in fragments shorter than    5611 bp
  65% of sequence in fragments shorter than    6210 bp
  70% of sequence in fragments shorter than    6884 bp
  75% of sequence in fragments shorter than    7670 bp
  80% of sequence in fragments shorter than    8633 bp
  85% of sequence in fragments shorter than    9859 bp
  90% of sequence in fragments shorter than   11623 bp
  95% of sequence in fragments shorter than   14846 bp

# ApaLI.AvaIII (GTGCAC+ATGCAT) double digest, 1429 thousand fragments (496255+933357 sites), 2085 bp average fragment length
   5% of sequence in fragments shorter than     745 bp
  10% of sequence in fragments shorter than    1132 bp
  15% of sequence in fragments shorter than    1462 bp
  20% of sequence in fragments shorter than    1774 bp
  25% of sequence in fragments shorter than    2076 bp
  30% of sequence in fragments shorter than    2371 bp
  35% of sequence in fragments shorter than    2677 bp
  40% of sequence in fragments shorter than    2992 bp
  45% of sequence in fragments shorter than    3320 bp
  50% of sequence in fragments shorter than    3674 bp
  55% of sequence in fragments shorter than    4054 bp
  60% of sequence in fragments shorter than    4472 bp
  65% of sequence in fragments shorter than    4923 bp
  70% of sequence in fragments shorter than    5444 bp
  75% of sequence in fragments shorter than    6039 bp
  80% of sequence in fragments shorter than    6750 bp
  85% of sequence in fragments shorter than    7672 bp
  90% of sequence in fragments shorter than    8994 bp
  95% of sequence in fragments shorter than   11518 bp

# BspHI.EcoRV (TCATGA+GATATC) double digest, 1424 thousand fragments (978290+446473 sites), 2092 bp average fragment length
   5% of sequence in fragments shorter than     730 bp
  10% of sequence in fragments shorter than    1088 bp
  15% of sequence in fragments shorter than    1418 bp
  20% of sequence in fragments shorter than    1731 bp
  25% of sequence in fragments shorter than    2026 bp
  30% of sequence in fragments shorter than    2324 bp
  35% of sequence in fragments shorter than    2633 bp
  40% of sequence in fragments shorter than    2953 bp
  45% of sequence in fragments shorter than    3292 bp
  50% of sequence in fragments shorter than    3650 bp
  55% of sequence in fragments shorter than    4037 bp
  60% of sequence in fragments shorter than    4460 bp
  65% of sequence in fragments shorter than    4937 bp
  70% of sequence in fragments shorter than    5483 bp
  75% of sequence in fragments shorter than    6116 bp
  80% of sequence in fragments shorter than    6898 bp
  85% of sequence in fragments shorter than    7928 bp
  90% of sequence in fragments shorter than    9457 bp
  95% of sequence in fragments shorter than   12471 bp

# HindIII.MfeI (AAGCTT+CAATTG) double digest, 1424 thousand fragments (860365+564303 sites), 2092 bp average fragment length
   5% of sequence in fragments shorter than     712 bp
  10% of sequence in fragments shorter than    1095 bp
  15% of sequence in fragments shorter than    1422 bp
  20% of sequence in fragments shorter than    1738 bp
  25% of sequence in fragments shorter than    2011 bp
  30% of sequence in fragments shorter than    2308 bp
  35% of sequence in fragments shorter than    2611 bp
  40% of sequence in fragments shorter than    2929 bp
  45% of sequence in fragments shorter than    3257 bp
  50% of sequence in fragments shorter than    3616 bp
  55% of sequence in fragments shorter than    3998 bp
  60% of sequence in fragments shorter than    4419 bp
  65% of sequence in fragments shorter than    4888 bp
  70% of sequence in fragments shorter than    5426 bp
  75% of sequence in fragments shorter than    6063 bp
  80% of sequence in fragments shorter than    6846 bp
  85% of sequence in fragments shorter than    7874 bp
  90% of sequence in fragments shorter than    9388 bp
  95% of sequence in fragments shorter than   12344 bp

# HgaI.XbaI (GACGC+TCTAGA) double digest, 1422 thousand fragments (571889+850998 sites), 2095 bp average fragment length
   5% of sequence in fragments shorter than     721 bp
  10% of sequence in fragments shorter than    1115 bp
  15% of sequence in fragments shorter than    1451 bp
  20% of sequence in fragments shorter than    1784 bp
  25% of sequence in fragments shorter than    2107 bp
  30% of sequence in fragments shorter than    2420 bp
  35% of sequence in fragments shorter than    2749 bp
  40% of sequence in fragments shorter than    3080 bp
  45% of sequence in fragments shorter than    3430 bp
  50% of sequence in fragments shorter than    3810 bp
  55% of sequence in fragments shorter than    4214 bp
  60% of sequence in fragments shorter than    4654 bp
  65% of sequence in fragments shorter than    5138 bp
  70% of sequence in fragments shorter than    5678 bp
  75% of sequence in fragments shorter than    6307 bp
  80% of sequence in fragments shorter than    7055 bp
  85% of sequence in fragments shorter than    8023 bp
  90% of sequence in fragments shorter than    9361 bp
  95% of sequence in fragments shorter than   11688 bp

# EciI.XbaI (GGCGGA+TCTAGA) double digest, 1421 thousand fragments (571273+850998 sites), 2095 bp average fragment length
   5% of sequence in fragments shorter than     724 bp
  10% of sequence in fragments shorter than    1096 bp
  15% of sequence in fragments shorter than    1419 bp
  20% of sequence in fragments shorter than    1740 bp
  25% of sequence in fragments shorter than    2055 bp
  30% of sequence in fragments shorter than    2358 bp
  35% of sequence in fragments shorter than    2680 bp
  40% of sequence in fragments shorter than    3009 bp
  45% of sequence in fragments shorter than    3356 bp
  50% of sequence in fragments shorter than    3722 bp
  55% of sequence in fragments shorter than    4117 bp
  60% of sequence in fragments shorter than    4550 bp
  65% of sequence in fragments shorter than    5027 bp
  70% of sequence in fragments shorter than    5556 bp
  75% of sequence in fragments shorter than    6179 bp
  80% of sequence in fragments shorter than    6924 bp
  85% of sequence in fragments shorter than    7879 bp
  90% of sequence in fragments shorter than    9224 bp
  95% of sequence in fragments shorter than   11617 bp

# ApaLI.StuI (GTGCAC+AGGCCT) double digest, 1421 thousand fragments (496255+925729 sites), 2096 bp average fragment length
   5% of sequence in fragments shorter than     686 bp
  10% of sequence in fragments shorter than    1060 bp
  15% of sequence in fragments shorter than    1429 bp
  20% of sequence in fragments shorter than    1788 bp
  25% of sequence in fragments shorter than    2123 bp
  30% of sequence in fragments shorter than    2460 bp
  35% of sequence in fragments shorter than    2832 bp
  40% of sequence in fragments shorter than    3217 bp
  45% of sequence in fragments shorter than    3618 bp
  50% of sequence in fragments shorter than    4046 bp
  55% of sequence in fragments shorter than    4487 bp
  60% of sequence in fragments shorter than    4997 bp
  65% of sequence in fragments shorter than    5556 bp
  70% of sequence in fragments shorter than    6182 bp
  75% of sequence in fragments shorter than    6923 bp
  80% of sequence in fragments shorter than    7811 bp
  85% of sequence in fragments shorter than    8925 bp
  90% of sequence in fragments shorter than   10489 bp
  95% of sequence in fragments shorter than   13134 bp

# BstEII.PpuMI (GGTNACC+RGGWCCY) double digest, 1420 thousand fragments (335865+1085138 sites), 2097 bp average fragment length
   5% of sequence in fragments shorter than     683 bp
  10% of sequence in fragments shorter than    1086 bp
  15% of sequence in fragments shorter than    1470 bp
  20% of sequence in fragments shorter than    1841 bp
  25% of sequence in fragments shorter than    2207 bp
  30% of sequence in fragments shorter than    2586 bp
  35% of sequence in fragments shorter than    2942 bp
  40% of sequence in fragments shorter than    3352 bp
  45% of sequence in fragments shorter than    3783 bp
  50% of sequence in fragments shorter than    4252 bp
  55% of sequence in fragments shorter than    4770 bp
  60% of sequence in fragments shorter than    5337 bp
  65% of sequence in fragments shorter than    5974 bp
  70% of sequence in fragments shorter than    6705 bp
  75% of sequence in fragments shorter than    7577 bp
  80% of sequence in fragments shorter than    8641 bp
  85% of sequence in fragments shorter than   10063 bp
  90% of sequence in fragments shorter than   12194 bp
  95% of sequence in fragments shorter than   16648 bp

# BstEII.PvuII (GGTNACC+CAGCTG) double digest, 1419 thousand fragments (335865+1084260 sites), 2098 bp average fragment length
   5% of sequence in fragments shorter than     708 bp
  10% of sequence in fragments shorter than    1092 bp
  15% of sequence in fragments shorter than    1446 bp
  20% of sequence in fragments shorter than    1789 bp
  25% of sequence in fragments shorter than    2131 bp
  30% of sequence in fragments shorter than    2480 bp
  35% of sequence in fragments shorter than    2838 bp
  40% of sequence in fragments shorter than    3211 bp
  45% of sequence in fragments shorter than    3614 bp
  50% of sequence in fragments shorter than    4050 bp
  55% of sequence in fragments shorter than    4523 bp
  60% of sequence in fragments shorter than    5037 bp
  65% of sequence in fragments shorter than    5613 bp
  70% of sequence in fragments shorter than    6263 bp
  75% of sequence in fragments shorter than    7021 bp
  80% of sequence in fragments shorter than    7962 bp
  85% of sequence in fragments shorter than    9208 bp
  90% of sequence in fragments shorter than   11068 bp
  95% of sequence in fragments shorter than   14763 bp

# EcoRI.HgaI (GAATTC+GACGC) double digest, 1418 thousand fragments (847401+571889 sites), 2100 bp average fragment length
   5% of sequence in fragments shorter than     692 bp
  10% of sequence in fragments shorter than    1121 bp
  15% of sequence in fragments shorter than    1484 bp
  20% of sequence in fragments shorter than    1834 bp
  25% of sequence in fragments shorter than    2172 bp
  30% of sequence in fragments shorter than    2506 bp
  35% of sequence in fragments shorter than    2835 bp
  40% of sequence in fragments shorter than    3181 bp
  45% of sequence in fragments shorter than    3533 bp
  50% of sequence in fragments shorter than    3918 bp
  55% of sequence in fragments shorter than    4326 bp
  60% of sequence in fragments shorter than    4776 bp
  65% of sequence in fragments shorter than    5271 bp
  70% of sequence in fragments shorter than    5831 bp
  75% of sequence in fragments shorter than    6485 bp
  80% of sequence in fragments shorter than    7262 bp
  85% of sequence in fragments shorter than    8250 bp
  90% of sequence in fragments shorter than    9632 bp
  95% of sequence in fragments shorter than   12080 bp

# BceAI.BsaAI (ACGGC+YACGTR) double digest, 1418 thousand fragments (705178+713801 sites), 2100 bp average fragment length
   5% of sequence in fragments shorter than     712 bp
  10% of sequence in fragments shorter than    1112 bp
  15% of sequence in fragments shorter than    1477 bp
  20% of sequence in fragments shorter than    1832 bp
  25% of sequence in fragments shorter than    2176 bp
  30% of sequence in fragments shorter than    2535 bp
  35% of sequence in fragments shorter than    2901 bp
  40% of sequence in fragments shorter than    3282 bp
  45% of sequence in fragments shorter than    3671 bp
  50% of sequence in fragments shorter than    4100 bp
  55% of sequence in fragments shorter than    4555 bp
  60% of sequence in fragments shorter than    5061 bp
  65% of sequence in fragments shorter than    5627 bp
  70% of sequence in fragments shorter than    6273 bp
  75% of sequence in fragments shorter than    7028 bp
  80% of sequence in fragments shorter than    7944 bp
  85% of sequence in fragments shorter than    9121 bp
  90% of sequence in fragments shorter than   10800 bp
  95% of sequence in fragments shorter than   13878 bp

# EciI.EcoRI (GGCGGA+GAATTC) double digest, 1418 thousand fragments (571273+847401 sites), 2101 bp average fragment length
   5% of sequence in fragments shorter than     692 bp
  10% of sequence in fragments shorter than    1101 bp
  15% of sequence in fragments shorter than    1457 bp
  20% of sequence in fragments shorter than    1795 bp
  25% of sequence in fragments shorter than    2124 bp
  30% of sequence in fragments shorter than    2452 bp
  35% of sequence in fragments shorter than    2781 bp
  40% of sequence in fragments shorter than    3124 bp
  45% of sequence in fragments shorter than    3472 bp
  50% of sequence in fragments shorter than    3852 bp
  55% of sequence in fragments shorter than    4259 bp
  60% of sequence in fragments shorter than    4708 bp
  65% of sequence in fragments shorter than    5206 bp
  70% of sequence in fragments shorter than    5768 bp
  75% of sequence in fragments shorter than    6421 bp
  80% of sequence in fragments shorter than    7221 bp
  85% of sequence in fragments shorter than    8223 bp
  90% of sequence in fragments shorter than    9648 bp
  95% of sequence in fragments shorter than   12165 bp

# AflII.BglII (CTTAAG+AGATCT) double digest, 1417 thousand fragments (643284+774732 sites), 2102 bp average fragment length
   5% of sequence in fragments shorter than     742 bp
  10% of sequence in fragments shorter than    1115 bp
  15% of sequence in fragments shorter than    1426 bp
  20% of sequence in fragments shorter than    1720 bp
  25% of sequence in fragments shorter than    2004 bp
  30% of sequence in fragments shorter than    2299 bp
  35% of sequence in fragments shorter than    2598 bp
  40% of sequence in fragments shorter than    2909 bp
  45% of sequence in fragments shorter than    3237 bp
  50% of sequence in fragments shorter than    3586 bp
  55% of sequence in fragments shorter than    3958 bp
  60% of sequence in fragments shorter than    4368 bp
  65% of sequence in fragments shorter than    4821 bp
  70% of sequence in fragments shorter than    5346 bp
  75% of sequence in fragments shorter than    5970 bp
  80% of sequence in fragments shorter than    6733 bp
  85% of sequence in fragments shorter than    7727 bp
  90% of sequence in fragments shorter than    9231 bp
  95% of sequence in fragments shorter than   12477 bp

# MfeI.XbaI (CAATTG+TCTAGA) double digest, 1414 thousand fragments (564303+850998 sites), 2106 bp average fragment length
   5% of sequence in fragments shorter than     734 bp
  10% of sequence in fragments shorter than    1084 bp
  15% of sequence in fragments shorter than    1395 bp
  20% of sequence in fragments shorter than    1718 bp
  25% of sequence in fragments shorter than    2026 bp
  30% of sequence in fragments shorter than    2335 bp
  35% of sequence in fragments shorter than    2659 bp
  40% of sequence in fragments shorter than    2989 bp
  45% of sequence in fragments shorter than    3337 bp
  50% of sequence in fragments shorter than    3700 bp
  55% of sequence in fragments shorter than    4089 bp
  60% of sequence in fragments shorter than    4522 bp
  65% of sequence in fragments shorter than    5004 bp
  70% of sequence in fragments shorter than    5551 bp
  75% of sequence in fragments shorter than    6201 bp
  80% of sequence in fragments shorter than    6998 bp
  85% of sequence in fragments shorter than    8031 bp
  90% of sequence in fragments shorter than    9543 bp
  95% of sequence in fragments shorter than   12377 bp

# EcoRI.MfeI (GAATTC+CAATTG) double digest, 1411 thousand fragments (847401+564303 sites), 2111 bp average fragment length
   5% of sequence in fragments shorter than     705 bp
  10% of sequence in fragments shorter than    1097 bp
  15% of sequence in fragments shorter than    1446 bp
  20% of sequence in fragments shorter than    1773 bp
  25% of sequence in fragments shorter than    2083 bp
  30% of sequence in fragments shorter than    2396 bp
  35% of sequence in fragments shorter than    2718 bp
  40% of sequence in fragments shorter than    3048 bp
  45% of sequence in fragments shorter than    3389 bp
  50% of sequence in fragments shorter than    3759 bp
  55% of sequence in fragments shorter than    4156 bp
  60% of sequence in fragments shorter than    4583 bp
  65% of sequence in fragments shorter than    5066 bp
  70% of sequence in fragments shorter than    5618 bp
  75% of sequence in fragments shorter than    6267 bp
  80% of sequence in fragments shorter than    7063 bp
  85% of sequence in fragments shorter than    8111 bp
  90% of sequence in fragments shorter than    9654 bp
  95% of sequence in fragments shorter than   12530 bp

# HindIII.SphI (AAGCTT+GCATGC) double digest, 1410 thousand fragments (860365+550952 sites), 2112 bp average fragment length
   5% of sequence in fragments shorter than     739 bp
  10% of sequence in fragments shorter than    1120 bp
  15% of sequence in fragments shorter than    1441 bp
  20% of sequence in fragments shorter than    1753 bp
  25% of sequence in fragments shorter than    2026 bp
  30% of sequence in fragments shorter than    2324 bp
  35% of sequence in fragments shorter than    2625 bp
  40% of sequence in fragments shorter than    2932 bp
  45% of sequence in fragments shorter than    3252 bp
  50% of sequence in fragments shorter than    3595 bp
  55% of sequence in fragments shorter than    3961 bp
  60% of sequence in fragments shorter than    4359 bp
  65% of sequence in fragments shorter than    4795 bp
  70% of sequence in fragments shorter than    5291 bp
  75% of sequence in fragments shorter than    5862 bp
  80% of sequence in fragments shorter than    6553 bp
  85% of sequence in fragments shorter than    7437 bp
  90% of sequence in fragments shorter than    8706 bp
  95% of sequence in fragments shorter than   11043 bp

# BciVI.BsaAI (GTATCC+YACGTR) double digest, 1409 thousand fragments (696093+713801 sites), 2114 bp average fragment length
   5% of sequence in fragments shorter than     769 bp
  10% of sequence in fragments shorter than    1145 bp
  15% of sequence in fragments shorter than    1461 bp
  20% of sequence in fragments shorter than    1767 bp
  25% of sequence in fragments shorter than    2067 bp
  30% of sequence in fragments shorter than    2368 bp
  35% of sequence in fragments shorter than    2681 bp
  40% of sequence in fragments shorter than    2997 bp
  45% of sequence in fragments shorter than    3326 bp
  50% of sequence in fragments shorter than    3677 bp
  55% of sequence in fragments shorter than    4055 bp
  60% of sequence in fragments shorter than    4466 bp
  65% of sequence in fragments shorter than    4914 bp
  70% of sequence in fragments shorter than    5427 bp
  75% of sequence in fragments shorter than    6009 bp
  80% of sequence in fragments shorter than    6724 bp
  85% of sequence in fragments shorter than    7633 bp
  90% of sequence in fragments shorter than    8919 bp
  95% of sequence in fragments shorter than   11188 bp

# ClaI.PstI (ATCGAT+CTGCAG) double digest, 1407 thousand fragments (86477+1321469 sites), 2117 bp average fragment length
   5% of sequence in fragments shorter than     698 bp
  10% of sequence in fragments shorter than    1099 bp
  15% of sequence in fragments shorter than    1449 bp
  20% of sequence in fragments shorter than    1796 bp
  25% of sequence in fragments shorter than    2128 bp
  30% of sequence in fragments shorter than    2475 bp
  35% of sequence in fragments shorter than    2832 bp
  40% of sequence in fragments shorter than    3203 bp
  45% of sequence in fragments shorter than    3600 bp
  50% of sequence in fragments shorter than    4029 bp
  55% of sequence in fragments shorter than    4497 bp
  60% of sequence in fragments shorter than    5007 bp
  65% of sequence in fragments shorter than    5575 bp
  70% of sequence in fragments shorter than    6223 bp
  75% of sequence in fragments shorter than    6984 bp
  80% of sequence in fragments shorter than    7914 bp
  85% of sequence in fragments shorter than    9114 bp
  90% of sequence in fragments shorter than   10830 bp
  95% of sequence in fragments shorter than   14086 bp

# AvaI.SpeI (CYCGRG+ACTAGT) double digest, 1405 thousand fragments (1005714+400286 sites), 2120 bp average fragment length
   5% of sequence in fragments shorter than     720 bp
  10% of sequence in fragments shorter than    1133 bp
  15% of sequence in fragments shorter than    1505 bp
  20% of sequence in fragments shorter than    1866 bp
  25% of sequence in fragments shorter than    2221 bp
  30% of sequence in fragments shorter than    2589 bp
  35% of sequence in fragments shorter than    2968 bp
  40% of sequence in fragments shorter than    3368 bp
  45% of sequence in fragments shorter than    3790 bp
  50% of sequence in fragments shorter than    4203 bp
  55% of sequence in fragments shorter than    4686 bp
  60% of sequence in fragments shorter than    5219 bp
  65% of sequence in fragments shorter than    5820 bp
  70% of sequence in fragments shorter than    6521 bp
  75% of sequence in fragments shorter than    7342 bp
  80% of sequence in fragments shorter than    8342 bp
  85% of sequence in fragments shorter than    9684 bp
  90% of sequence in fragments shorter than   11628 bp
  95% of sequence in fragments shorter than   15385 bp

# BglII.HaeII (AGATCT+RGCGCY) double digest, 1404 thousand fragments (774732+630101 sites), 2121 bp average fragment length
   5% of sequence in fragments shorter than     691 bp
  10% of sequence in fragments shorter than    1106 bp
  15% of sequence in fragments shorter than    1476 bp
  20% of sequence in fragments shorter than    1822 bp
  25% of sequence in fragments shorter than    2151 bp
  30% of sequence in fragments shorter than    2499 bp
  35% of sequence in fragments shorter than    2854 bp
  40% of sequence in fragments shorter than    3218 bp
  45% of sequence in fragments shorter than    3591 bp
  50% of sequence in fragments shorter than    3991 bp
  55% of sequence in fragments shorter than    4411 bp
  60% of sequence in fragments shorter than    4873 bp
  65% of sequence in fragments shorter than    5378 bp
  70% of sequence in fragments shorter than    5948 bp
  75% of sequence in fragments shorter than    6609 bp
  80% of sequence in fragments shorter than    7403 bp
  85% of sequence in fragments shorter than    8409 bp
  90% of sequence in fragments shorter than    9827 bp
  95% of sequence in fragments shorter than   12271 bp

# HindIII.ScaI (AAGCTT+AGTACT) double digest, 1403 thousand fragments (860365+543793 sites), 2122 bp average fragment length
   5% of sequence in fragments shorter than     736 bp
  10% of sequence in fragments shorter than    1116 bp
  15% of sequence in fragments shorter than    1442 bp
  20% of sequence in fragments shorter than    1758 bp
  25% of sequence in fragments shorter than    2038 bp
  30% of sequence in fragments shorter than    2345 bp
  35% of sequence in fragments shorter than    2651 bp
  40% of sequence in fragments shorter than    2963 bp
  45% of sequence in fragments shorter than    3299 bp
  50% of sequence in fragments shorter than    3658 bp
  55% of sequence in fragments shorter than    4040 bp
  60% of sequence in fragments shorter than    4461 bp
  65% of sequence in fragments shorter than    4926 bp
  70% of sequence in fragments shorter than    5460 bp
  75% of sequence in fragments shorter than    6100 bp
  80% of sequence in fragments shorter than    6875 bp
  85% of sequence in fragments shorter than    7914 bp
  90% of sequence in fragments shorter than    9466 bp
  95% of sequence in fragments shorter than   12592 bp

# AclI.BscGI (AACGTT+CCCGT) double digest, 1403 thousand fragments (172706+1231070 sites), 2123 bp average fragment length
   5% of sequence in fragments shorter than     625 bp
  10% of sequence in fragments shorter than    1073 bp
  15% of sequence in fragments shorter than    1496 bp
  20% of sequence in fragments shorter than    1910 bp
  25% of sequence in fragments shorter than    2322 bp
  30% of sequence in fragments shorter than    2741 bp
  35% of sequence in fragments shorter than    3179 bp
  40% of sequence in fragments shorter than    3623 bp
  45% of sequence in fragments shorter than    4110 bp
  50% of sequence in fragments shorter than    4630 bp
  55% of sequence in fragments shorter than    5203 bp
  60% of sequence in fragments shorter than    5825 bp
  65% of sequence in fragments shorter than    6529 bp
  70% of sequence in fragments shorter than    7327 bp
  75% of sequence in fragments shorter than    8269 bp
  80% of sequence in fragments shorter than    9434 bp
  85% of sequence in fragments shorter than   10904 bp
  90% of sequence in fragments shorter than   12946 bp
  95% of sequence in fragments shorter than   16471 bp

# AflII.NcoI (CTTAAG+CCATGG) double digest, 1402 thousand fragments (643284+759594 sites), 2124 bp average fragment length
   5% of sequence in fragments shorter than     747 bp
  10% of sequence in fragments shorter than    1131 bp
  15% of sequence in fragments shorter than    1455 bp
  20% of sequence in fragments shorter than    1737 bp
  25% of sequence in fragments shorter than    2038 bp
  30% of sequence in fragments shorter than    2341 bp
  35% of sequence in fragments shorter than    2652 bp
  40% of sequence in fragments shorter than    2967 bp
  45% of sequence in fragments shorter than    3293 bp
  50% of sequence in fragments shorter than    3645 bp
  55% of sequence in fragments shorter than    4019 bp
  60% of sequence in fragments shorter than    4425 bp
  65% of sequence in fragments shorter than    4866 bp
  70% of sequence in fragments shorter than    5377 bp
  75% of sequence in fragments shorter than    5975 bp
  80% of sequence in fragments shorter than    6695 bp
  85% of sequence in fragments shorter than    7640 bp
  90% of sequence in fragments shorter than    9003 bp
  95% of sequence in fragments shorter than   11689 bp

# SphI.XbaI (GCATGC+TCTAGA) double digest, 1401 thousand fragments (550952+850998 sites), 2126 bp average fragment length
   5% of sequence in fragments shorter than     750 bp
  10% of sequence in fragments shorter than    1116 bp
  15% of sequence in fragments shorter than    1427 bp
  20% of sequence in fragments shorter than    1736 bp
  25% of sequence in fragments shorter than    2040 bp
  30% of sequence in fragments shorter than    2325 bp
  35% of sequence in fragments shorter than    2633 bp
  40% of sequence in fragments shorter than    2945 bp
  45% of sequence in fragments shorter than    3273 bp
  50% of sequence in fragments shorter than    3614 bp
  55% of sequence in fragments shorter than    3983 bp
  60% of sequence in fragments shorter than    4381 bp
  65% of sequence in fragments shorter than    4824 bp
  70% of sequence in fragments shorter than    5320 bp
  75% of sequence in fragments shorter than    5891 bp
  80% of sequence in fragments shorter than    6575 bp
  85% of sequence in fragments shorter than    7457 bp
  90% of sequence in fragments shorter than    8684 bp
  95% of sequence in fragments shorter than   10909 bp

# BceAI.BciVI (ACGGC+GTATCC) double digest, 1400 thousand fragments (705178+696093 sites), 2127 bp average fragment length
   5% of sequence in fragments shorter than     738 bp
  10% of sequence in fragments shorter than    1138 bp
  15% of sequence in fragments shorter than    1494 bp
  20% of sequence in fragments shorter than    1812 bp
  25% of sequence in fragments shorter than    2127 bp
  30% of sequence in fragments shorter than    2451 bp
  35% of sequence in fragments shorter than    2783 bp
  40% of sequence in fragments shorter than    3131 bp
  45% of sequence in fragments shorter than    3497 bp
  50% of sequence in fragments shorter than    3877 bp
  55% of sequence in fragments shorter than    4282 bp
  60% of sequence in fragments shorter than    4730 bp
  65% of sequence in fragments shorter than    5231 bp
  70% of sequence in fragments shorter than    5799 bp
  75% of sequence in fragments shorter than    6454 bp
  80% of sequence in fragments shorter than    7264 bp
  85% of sequence in fragments shorter than    8282 bp
  90% of sequence in fragments shorter than    9726 bp
  95% of sequence in fragments shorter than   12409 bp

# ApaLI.NdeI (GTGCAC+CATATG) double digest, 1399 thousand fragments (496255+903360 sites), 2129 bp average fragment length
   5% of sequence in fragments shorter than     758 bp
  10% of sequence in fragments shorter than    1149 bp
  15% of sequence in fragments shorter than    1477 bp
  20% of sequence in fragments shorter than    1808 bp
  25% of sequence in fragments shorter than    2130 bp
  30% of sequence in fragments shorter than    2452 bp
  35% of sequence in fragments shorter than    2780 bp
  40% of sequence in fragments shorter than    3119 bp
  45% of sequence in fragments shorter than    3469 bp
  50% of sequence in fragments shorter than    3844 bp
  55% of sequence in fragments shorter than    4245 bp
  60% of sequence in fragments shorter than    4667 bp
  65% of sequence in fragments shorter than    5142 bp
  70% of sequence in fragments shorter than    5681 bp
  75% of sequence in fragments shorter than    6309 bp
  80% of sequence in fragments shorter than    7075 bp
  85% of sequence in fragments shorter than    8070 bp
  90% of sequence in fragments shorter than    9504 bp
  95% of sequence in fragments shorter than   12301 bp

# AccI.SexAI (GTMKAC+ACCWGGT) double digest, 1398 thousand fragments (1101063+297900 sites), 2130 bp average fragment length
   5% of sequence in fragments shorter than     755 bp
  10% of sequence in fragments shorter than    1145 bp
  15% of sequence in fragments shorter than    1466 bp
  20% of sequence in fragments shorter than    1778 bp
  25% of sequence in fragments shorter than    2076 bp
  30% of sequence in fragments shorter than    2378 bp
  35% of sequence in fragments shorter than    2682 bp
  40% of sequence in fragments shorter than    2993 bp
  45% of sequence in fragments shorter than    3326 bp
  50% of sequence in fragments shorter than    3673 bp
  55% of sequence in fragments shorter than    4049 bp
  60% of sequence in fragments shorter than    4457 bp
  65% of sequence in fragments shorter than    4902 bp
  70% of sequence in fragments shorter than    5416 bp
  75% of sequence in fragments shorter than    5983 bp
  80% of sequence in fragments shorter than    6637 bp
  85% of sequence in fragments shorter than    7533 bp
  90% of sequence in fragments shorter than    8804 bp
  95% of sequence in fragments shorter than   11137 bp

# EcoRI.SphI (GAATTC+GCATGC) double digest, 1397 thousand fragments (847401+550952 sites), 2131 bp average fragment length
   5% of sequence in fragments shorter than     716 bp
  10% of sequence in fragments shorter than    1121 bp
  15% of sequence in fragments shorter than    1464 bp
  20% of sequence in fragments shorter than    1788 bp
  25% of sequence in fragments shorter than    2099 bp
  30% of sequence in fragments shorter than    2409 bp
  35% of sequence in fragments shorter than    2723 bp
  40% of sequence in fragments shorter than    3039 bp
  45% of sequence in fragments shorter than    3371 bp
  50% of sequence in fragments shorter than    3722 bp
  55% of sequence in fragments shorter than    4096 bp
  60% of sequence in fragments shorter than    4505 bp
  65% of sequence in fragments shorter than    4958 bp
  70% of sequence in fragments shorter than    5467 bp
  75% of sequence in fragments shorter than    6058 bp
  80% of sequence in fragments shorter than    6774 bp
  85% of sequence in fragments shorter than    7673 bp
  90% of sequence in fragments shorter than    8935 bp
  95% of sequence in fragments shorter than   11156 bp

# AvaI.HpaI (CYCGRG+GTTAAC) double digest, 1394 thousand fragments (1005714+389418 sites), 2136 bp average fragment length
   5% of sequence in fragments shorter than     716 bp
  10% of sequence in fragments shorter than    1130 bp
  15% of sequence in fragments shorter than    1511 bp
  20% of sequence in fragments shorter than    1881 bp
  25% of sequence in fragments shorter than    2250 bp
  30% of sequence in fragments shorter than    2631 bp
  35% of sequence in fragments shorter than    3028 bp
  40% of sequence in fragments shorter than    3446 bp
  45% of sequence in fragments shorter than    3887 bp
  50% of sequence in fragments shorter than    4363 bp
  55% of sequence in fragments shorter than    4884 bp
  60% of sequence in fragments shorter than    5452 bp
  65% of sequence in fragments shorter than    6105 bp
  70% of sequence in fragments shorter than    6846 bp
  75% of sequence in fragments shorter than    7726 bp
  80% of sequence in fragments shorter than    8822 bp
  85% of sequence in fragments shorter than   10261 bp
  90% of sequence in fragments shorter than   12405 bp
  95% of sequence in fragments shorter than   16753 bp

# ScaI.XbaI (AGTACT+TCTAGA) double digest, 1394 thousand fragments (543793+850998 sites), 2137 bp average fragment length
   5% of sequence in fragments shorter than     745 bp
  10% of sequence in fragments shorter than    1108 bp
  15% of sequence in fragments shorter than    1422 bp
  20% of sequence in fragments shorter than    1736 bp
  25% of sequence in fragments shorter than    2049 bp
  30% of sequence in fragments shorter than    2349 bp
  35% of sequence in fragments shorter than    2667 bp
  40% of sequence in fragments shorter than    2996 bp
  45% of sequence in fragments shorter than    3342 bp
  50% of sequence in fragments shorter than    3707 bp
  55% of sequence in fragments shorter than    4097 bp
  60% of sequence in fragments shorter than    4529 bp
  65% of sequence in fragments shorter than    5014 bp
  70% of sequence in fragments shorter than    5567 bp
  75% of sequence in fragments shorter than    6209 bp
  80% of sequence in fragments shorter than    6999 bp
  85% of sequence in fragments shorter than    8021 bp
  90% of sequence in fragments shorter than    9527 bp
  95% of sequence in fragments shorter than   12415 bp

# ApaI.AvaIII (GGGCCC+ATGCAT) double digest, 1393 thousand fragments (460339+933357 sites), 2138 bp average fragment length
   5% of sequence in fragments shorter than     743 bp
  10% of sequence in fragments shorter than    1146 bp
  15% of sequence in fragments shorter than    1494 bp
  20% of sequence in fragments shorter than    1828 bp
  25% of sequence in fragments shorter than    2147 bp
  30% of sequence in fragments shorter than    2473 bp
  35% of sequence in fragments shorter than    2802 bp
  40% of sequence in fragments shorter than    3151 bp
  45% of sequence in fragments shorter than    3516 bp
  50% of sequence in fragments shorter than    3910 bp
  55% of sequence in fragments shorter than    4335 bp
  60% of sequence in fragments shorter than    4805 bp
  65% of sequence in fragments shorter than    5320 bp
  70% of sequence in fragments shorter than    5919 bp
  75% of sequence in fragments shorter than    6610 bp
  80% of sequence in fragments shorter than    7470 bp
  85% of sequence in fragments shorter than    8569 bp
  90% of sequence in fragments shorter than   10166 bp
  95% of sequence in fragments shorter than   13216 bp

# AatII.PstI (GACGTC+CTGCAG) double digest, 1392 thousand fragments (71089+1321469 sites), 2140 bp average fragment length
   5% of sequence in fragments shorter than     700 bp
  10% of sequence in fragments shorter than    1106 bp
  15% of sequence in fragments shorter than    1465 bp
  20% of sequence in fragments shorter than    1821 bp
  25% of sequence in fragments shorter than    2167 bp
  30% of sequence in fragments shorter than    2528 bp
  35% of sequence in fragments shorter than    2904 bp
  40% of sequence in fragments shorter than    3296 bp
  45% of sequence in fragments shorter than    3717 bp
  50% of sequence in fragments shorter than    4167 bp
  55% of sequence in fragments shorter than    4664 bp
  60% of sequence in fragments shorter than    5200 bp
  65% of sequence in fragments shorter than    5799 bp
  70% of sequence in fragments shorter than    6481 bp
  75% of sequence in fragments shorter than    7286 bp
  80% of sequence in fragments shorter than    8275 bp
  85% of sequence in fragments shorter than    9556 bp
  90% of sequence in fragments shorter than   11406 bp
  95% of sequence in fragments shorter than   14884 bp

# EcoRI.ScaI (GAATTC+AGTACT) double digest, 1390 thousand fragments (847401+543793 sites), 2142 bp average fragment length
   5% of sequence in fragments shorter than     709 bp
  10% of sequence in fragments shorter than    1118 bp
  15% of sequence in fragments shorter than    1468 bp
  20% of sequence in fragments shorter than    1798 bp
  25% of sequence in fragments shorter than    2118 bp
  30% of sequence in fragments shorter than    2438 bp
  35% of sequence in fragments shorter than    2758 bp
  40% of sequence in fragments shorter than    3091 bp
  45% of sequence in fragments shorter than    3435 bp
  50% of sequence in fragments shorter than    3805 bp
  55% of sequence in fragments shorter than    4202 bp
  60% of sequence in fragments shorter than    4633 bp
  65% of sequence in fragments shorter than    5119 bp
  70% of sequence in fragments shorter than    5674 bp
  75% of sequence in fragments shorter than    6321 bp
  80% of sequence in fragments shorter than    7116 bp
  85% of sequence in fragments shorter than    8122 bp
  90% of sequence in fragments shorter than    9578 bp
  95% of sequence in fragments shorter than   12291 bp

# BscGI.PacI (CCCGT+TTAATTAA) double digest, 1390 thousand fragments (1231070+159455 sites), 2143 bp average fragment length
   5% of sequence in fragments shorter than     643 bp
  10% of sequence in fragments shorter than    1092 bp
  15% of sequence in fragments shorter than    1518 bp
  20% of sequence in fragments shorter than    1931 bp
  25% of sequence in fragments shorter than    2343 bp
  30% of sequence in fragments shorter than    2761 bp
  35% of sequence in fragments shorter than    3200 bp
  40% of sequence in fragments shorter than    3652 bp
  45% of sequence in fragments shorter than    4136 bp
  50% of sequence in fragments shorter than    4650 bp
  55% of sequence in fragments shorter than    5215 bp
  60% of sequence in fragments shorter than    5833 bp
  65% of sequence in fragments shorter than    6516 bp
  70% of sequence in fragments shorter than    7304 bp
  75% of sequence in fragments shorter than    8218 bp
  80% of sequence in fragments shorter than    9349 bp
  85% of sequence in fragments shorter than   10800 bp
  90% of sequence in fragments shorter than   12794 bp
  95% of sequence in fragments shorter than   16263 bp

# HaeII.NcoI (RGCGCY+CCATGG) double digest, 1389 thousand fragments (630101+759594 sites), 2145 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1108 bp
  15% of sequence in fragments shorter than    1518 bp
  20% of sequence in fragments shorter than    1903 bp
  25% of sequence in fragments shorter than    2278 bp
  30% of sequence in fragments shorter than    2668 bp
  35% of sequence in fragments shorter than    3072 bp
  40% of sequence in fragments shorter than    3484 bp
  45% of sequence in fragments shorter than    3912 bp
  50% of sequence in fragments shorter than    4380 bp
  55% of sequence in fragments shorter than    4850 bp
  60% of sequence in fragments shorter than    5390 bp
  65% of sequence in fragments shorter than    5974 bp
  70% of sequence in fragments shorter than    6658 bp
  75% of sequence in fragments shorter than    7469 bp
  80% of sequence in fragments shorter than    8465 bp
  85% of sequence in fragments shorter than    9723 bp
  90% of sequence in fragments shorter than   11502 bp
  95% of sequence in fragments shorter than   14524 bp

# AccI.KpnI (GTMKAC+GGTACC) double digest, 1388 thousand fragments (1101063+287974 sites), 2146 bp average fragment length
   5% of sequence in fragments shorter than     772 bp
  10% of sequence in fragments shorter than    1167 bp
  15% of sequence in fragments shorter than    1444 bp
  20% of sequence in fragments shorter than    1749 bp
  25% of sequence in fragments shorter than    2047 bp
  30% of sequence in fragments shorter than    2355 bp
  35% of sequence in fragments shorter than    2661 bp
  40% of sequence in fragments shorter than    2974 bp
  45% of sequence in fragments shorter than    3312 bp
  50% of sequence in fragments shorter than    3663 bp
  55% of sequence in fragments shorter than    4050 bp
  60% of sequence in fragments shorter than    4466 bp
  65% of sequence in fragments shorter than    4934 bp
  70% of sequence in fragments shorter than    5456 bp
  75% of sequence in fragments shorter than    6070 bp
  80% of sequence in fragments shorter than    6818 bp
  85% of sequence in fragments shorter than    7773 bp
  90% of sequence in fragments shorter than    9137 bp
  95% of sequence in fragments shorter than   11669 bp

# BscGI.Hpy99I (CCCGT+CGWCG) double digest, 1388 thousand fragments (1231070+157917 sites), 2146 bp average fragment length
   5% of sequence in fragments shorter than     620 bp
  10% of sequence in fragments shorter than    1097 bp
  15% of sequence in fragments shorter than    1564 bp
  20% of sequence in fragments shorter than    2028 bp
  25% of sequence in fragments shorter than    2497 bp
  30% of sequence in fragments shorter than    2982 bp
  35% of sequence in fragments shorter than    3494 bp
  40% of sequence in fragments shorter than    4030 bp
  45% of sequence in fragments shorter than    4603 bp
  50% of sequence in fragments shorter than    5232 bp
  55% of sequence in fragments shorter than    5917 bp
  60% of sequence in fragments shorter than    6667 bp
  65% of sequence in fragments shorter than    7527 bp
  70% of sequence in fragments shorter than    8510 bp
  75% of sequence in fragments shorter than    9692 bp
  80% of sequence in fragments shorter than   11115 bp
  85% of sequence in fragments shorter than   12957 bp
  90% of sequence in fragments shorter than   15572 bp
  95% of sequence in fragments shorter than   19981 bp

# AccI.NheI (GTMKAC+GCTAGC) double digest, 1387 thousand fragments (1101063+287384 sites), 2146 bp average fragment length
   5% of sequence in fragments shorter than     767 bp
  10% of sequence in fragments shorter than    1158 bp
  15% of sequence in fragments shorter than    1463 bp
  20% of sequence in fragments shorter than    1765 bp
  25% of sequence in fragments shorter than    2064 bp
  30% of sequence in fragments shorter than    2374 bp
  35% of sequence in fragments shorter than    2673 bp
  40% of sequence in fragments shorter than    2985 bp
  45% of sequence in fragments shorter than    3327 bp
  50% of sequence in fragments shorter than    3689 bp
  55% of sequence in fragments shorter than    4080 bp
  60% of sequence in fragments shorter than    4504 bp
  65% of sequence in fragments shorter than    4977 bp
  70% of sequence in fragments shorter than    5510 bp
  75% of sequence in fragments shorter than    6134 bp
  80% of sequence in fragments shorter than    6890 bp
  85% of sequence in fragments shorter than    7868 bp
  90% of sequence in fragments shorter than    9276 bp
  95% of sequence in fragments shorter than   11846 bp

# PstI.SacII (CTGCAG+CCGCGG) double digest, 1387 thousand fragments (1321469+66901 sites), 2147 bp average fragment length
   5% of sequence in fragments shorter than     698 bp
  10% of sequence in fragments shorter than    1117 bp
  15% of sequence in fragments shorter than    1490 bp
  20% of sequence in fragments shorter than    1859 bp
  25% of sequence in fragments shorter than    2218 bp
  30% of sequence in fragments shorter than    2593 bp
  35% of sequence in fragments shorter than    2980 bp
  40% of sequence in fragments shorter than    3394 bp
  45% of sequence in fragments shorter than    3830 bp
  50% of sequence in fragments shorter than    4300 bp
  55% of sequence in fragments shorter than    4814 bp
  60% of sequence in fragments shorter than    5372 bp
  65% of sequence in fragments shorter than    5989 bp
  70% of sequence in fragments shorter than    6704 bp
  75% of sequence in fragments shorter than    7544 bp
  80% of sequence in fragments shorter than    8576 bp
  85% of sequence in fragments shorter than    9929 bp
  90% of sequence in fragments shorter than   11857 bp
  95% of sequence in fragments shorter than   15556 bp

# ApaI.StuI (GGGCCC+AGGCCT) double digest, 1385 thousand fragments (460339+925729 sites), 2150 bp average fragment length
   5% of sequence in fragments shorter than     662 bp
  10% of sequence in fragments shorter than    1067 bp
  15% of sequence in fragments shorter than    1486 bp
  20% of sequence in fragments shorter than    1934 bp
  25% of sequence in fragments shorter than    2353 bp
  30% of sequence in fragments shorter than    2809 bp
  35% of sequence in fragments shorter than    3289 bp
  40% of sequence in fragments shorter than    3780 bp
  45% of sequence in fragments shorter than    4302 bp
  50% of sequence in fragments shorter than    4864 bp
  55% of sequence in fragments shorter than    5475 bp
  60% of sequence in fragments shorter than    6139 bp
  65% of sequence in fragments shorter than    6882 bp
  70% of sequence in fragments shorter than    7724 bp
  75% of sequence in fragments shorter than    8708 bp
  80% of sequence in fragments shorter than    9908 bp
  85% of sequence in fragments shorter than   11421 bp
  90% of sequence in fragments shorter than   13542 bp
  95% of sequence in fragments shorter than   17224 bp

# PpuMI.SexAI (RGGWCCY+ACCWGGT) double digest, 1382 thousand fragments (1085138+297900 sites), 2155 bp average fragment length
   5% of sequence in fragments shorter than     708 bp
  10% of sequence in fragments shorter than    1137 bp
  15% of sequence in fragments shorter than    1530 bp
  20% of sequence in fragments shorter than    1906 bp
  25% of sequence in fragments shorter than    2283 bp
  30% of sequence in fragments shorter than    2671 bp
  35% of sequence in fragments shorter than    3078 bp
  40% of sequence in fragments shorter than    3490 bp
  45% of sequence in fragments shorter than    3889 bp
  50% of sequence in fragments shorter than    4364 bp
  55% of sequence in fragments shorter than    4892 bp
  60% of sequence in fragments shorter than    5470 bp
  65% of sequence in fragments shorter than    6120 bp
  70% of sequence in fragments shorter than    6876 bp
  75% of sequence in fragments shorter than    7762 bp
  80% of sequence in fragments shorter than    8847 bp
  85% of sequence in fragments shorter than   10286 bp
  90% of sequence in fragments shorter than   12395 bp
  95% of sequence in fragments shorter than   16760 bp

# AvaI.SapI (CYCGRG+GCTCTTC) double digest, 1382 thousand fragments (1005714+377161 sites), 2155 bp average fragment length
   5% of sequence in fragments shorter than     712 bp
  10% of sequence in fragments shorter than    1132 bp
  15% of sequence in fragments shorter than    1528 bp
  20% of sequence in fragments shorter than    1920 bp
  25% of sequence in fragments shorter than    2317 bp
  30% of sequence in fragments shorter than    2731 bp
  35% of sequence in fragments shorter than    3165 bp
  40% of sequence in fragments shorter than    3629 bp
  45% of sequence in fragments shorter than    4123 bp
  50% of sequence in fragments shorter than    4656 bp
  55% of sequence in fragments shorter than    5240 bp
  60% of sequence in fragments shorter than    5890 bp
  65% of sequence in fragments shorter than    6637 bp
  70% of sequence in fragments shorter than    7501 bp
  75% of sequence in fragments shorter than    8529 bp
  80% of sequence in fragments shorter than    9812 bp
  85% of sequence in fragments shorter than   11501 bp
  90% of sequence in fragments shorter than   14023 bp
  95% of sequence in fragments shorter than   19265 bp

# PvuII.SexAI (CAGCTG+ACCWGGT) double digest, 1381 thousand fragments (1084260+297900 sites), 2156 bp average fragment length
   5% of sequence in fragments shorter than     722 bp
  10% of sequence in fragments shorter than    1120 bp
  15% of sequence in fragments shorter than    1481 bp
  20% of sequence in fragments shorter than    1832 bp
  25% of sequence in fragments shorter than    2182 bp
  30% of sequence in fragments shorter than    2536 bp
  35% of sequence in fragments shorter than    2890 bp
  40% of sequence in fragments shorter than    3270 bp
  45% of sequence in fragments shorter than    3677 bp
  50% of sequence in fragments shorter than    4119 bp
  55% of sequence in fragments shorter than    4599 bp
  60% of sequence in fragments shorter than    5128 bp
  65% of sequence in fragments shorter than    5723 bp
  70% of sequence in fragments shorter than    6392 bp
  75% of sequence in fragments shorter than    7173 bp
  80% of sequence in fragments shorter than    8145 bp
  85% of sequence in fragments shorter than    9400 bp
  90% of sequence in fragments shorter than   11257 bp
  95% of sequence in fragments shorter than   15007 bp

# AflII.BclI (CTTAAG+TGATCA) double digest, 1381 thousand fragments (643284+738785 sites), 2156 bp average fragment length
   5% of sequence in fragments shorter than     751 bp
  10% of sequence in fragments shorter than    1132 bp
  15% of sequence in fragments shorter than    1465 bp
  20% of sequence in fragments shorter than    1778 bp
  25% of sequence in fragments shorter than    2085 bp
  30% of sequence in fragments shorter than    2388 bp
  35% of sequence in fragments shorter than    2690 bp
  40% of sequence in fragments shorter than    3007 bp
  45% of sequence in fragments shorter than    3339 bp
  50% of sequence in fragments shorter than    3697 bp
  55% of sequence in fragments shorter than    4077 bp
  60% of sequence in fragments shorter than    4495 bp
  65% of sequence in fragments shorter than    4959 bp
  70% of sequence in fragments shorter than    5488 bp
  75% of sequence in fragments shorter than    6118 bp
  80% of sequence in fragments shorter than    6896 bp
  85% of sequence in fragments shorter than    7919 bp
  90% of sequence in fragments shorter than    9465 bp
  95% of sequence in fragments shorter than   12794 bp

# PspXI.PstI (VCTCGAGB+CTGCAG) double digest, 1380 thousand fragments (59193+1321469 sites), 2159 bp average fragment length
   5% of sequence in fragments shorter than     705 bp
  10% of sequence in fragments shorter than    1116 bp
  15% of sequence in fragments shorter than    1479 bp
  20% of sequence in fragments shorter than    1840 bp
  25% of sequence in fragments shorter than    2191 bp
  30% of sequence in fragments shorter than    2558 bp
  35% of sequence in fragments shorter than    2934 bp
  40% of sequence in fragments shorter than    3336 bp
  45% of sequence in fragments shorter than    3762 bp
  50% of sequence in fragments shorter than    4220 bp
  55% of sequence in fragments shorter than    4723 bp
  60% of sequence in fragments shorter than    5267 bp
  65% of sequence in fragments shorter than    5876 bp
  70% of sequence in fragments shorter than    6569 bp
  75% of sequence in fragments shorter than    7389 bp
  80% of sequence in fragments shorter than    8392 bp
  85% of sequence in fragments shorter than    9705 bp
  90% of sequence in fragments shorter than   11607 bp
  95% of sequence in fragments shorter than   15171 bp

# AvaIII.EcoRV (ATGCAT+GATATC) double digest, 1379 thousand fragments (933357+446473 sites), 2160 bp average fragment length
   5% of sequence in fragments shorter than     752 bp
  10% of sequence in fragments shorter than    1148 bp
  15% of sequence in fragments shorter than    1495 bp
  20% of sequence in fragments shorter than    1824 bp
  25% of sequence in fragments shorter than    2136 bp
  30% of sequence in fragments shorter than    2452 bp
  35% of sequence in fragments shorter than    2775 bp
  40% of sequence in fragments shorter than    3118 bp
  45% of sequence in fragments shorter than    3475 bp
  50% of sequence in fragments shorter than    3855 bp
  55% of sequence in fragments shorter than    4264 bp
  60% of sequence in fragments shorter than    4718 bp
  65% of sequence in fragments shorter than    5223 bp
  70% of sequence in fragments shorter than    5811 bp
  75% of sequence in fragments shorter than    6506 bp
  80% of sequence in fragments shorter than    7374 bp
  85% of sequence in fragments shorter than    8528 bp
  90% of sequence in fragments shorter than   10245 bp
  95% of sequence in fragments shorter than   13755 bp

# BspHI.SpeI (TCATGA+ACTAGT) double digest, 1378 thousand fragments (978290+400286 sites), 2162 bp average fragment length
   5% of sequence in fragments shorter than     758 bp
  10% of sequence in fragments shorter than    1142 bp
  15% of sequence in fragments shorter than    1464 bp
  20% of sequence in fragments shorter than    1784 bp
  25% of sequence in fragments shorter than    2085 bp
  30% of sequence in fragments shorter than    2387 bp
  35% of sequence in fragments shorter than    2702 bp
  40% of sequence in fragments shorter than    3027 bp
  45% of sequence in fragments shorter than    3371 bp
  50% of sequence in fragments shorter than    3738 bp
  55% of sequence in fragments shorter than    4139 bp
  60% of sequence in fragments shorter than    4571 bp
  65% of sequence in fragments shorter than    5050 bp
  70% of sequence in fragments shorter than    5600 bp
  75% of sequence in fragments shorter than    6247 bp
  80% of sequence in fragments shorter than    7040 bp
  85% of sequence in fragments shorter than    8096 bp
  90% of sequence in fragments shorter than    9665 bp
  95% of sequence in fragments shorter than   12850 bp

# BglII.SacI (AGATCT+GAGCTC) double digest, 1377 thousand fragments (774732+603323 sites), 2163 bp average fragment length
   5% of sequence in fragments shorter than     755 bp
  10% of sequence in fragments shorter than    1138 bp
  15% of sequence in fragments shorter than    1461 bp
  20% of sequence in fragments shorter than    1781 bp
  25% of sequence in fragments shorter than    2087 bp
  30% of sequence in fragments shorter than    2397 bp
  35% of sequence in fragments shorter than    2713 bp
  40% of sequence in fragments shorter than    3043 bp
  45% of sequence in fragments shorter than    3386 bp
  50% of sequence in fragments shorter than    3746 bp
  55% of sequence in fragments shorter than    4134 bp
  60% of sequence in fragments shorter than    4551 bp
  65% of sequence in fragments shorter than    5015 bp
  70% of sequence in fragments shorter than    5545 bp
  75% of sequence in fragments shorter than    6165 bp
  80% of sequence in fragments shorter than    6909 bp
  85% of sequence in fragments shorter than    7869 bp
  90% of sequence in fragments shorter than    9274 bp
  95% of sequence in fragments shorter than   11942 bp

# AflII.FnuDII (CTTAAG+CGCG) double digest, 1376 thousand fragments (643284+733940 sites), 2164 bp average fragment length
   5% of sequence in fragments shorter than     844 bp
  10% of sequence in fragments shorter than    1368 bp
  15% of sequence in fragments shorter than    1821 bp
  20% of sequence in fragments shorter than    2240 bp
  25% of sequence in fragments shorter than    2647 bp
  30% of sequence in fragments shorter than    3056 bp
  35% of sequence in fragments shorter than    3477 bp
  40% of sequence in fragments shorter than    3899 bp
  45% of sequence in fragments shorter than    4340 bp
  50% of sequence in fragments shorter than    4818 bp
  55% of sequence in fragments shorter than    5333 bp
  60% of sequence in fragments shorter than    5899 bp
  65% of sequence in fragments shorter than    6533 bp
  70% of sequence in fragments shorter than    7250 bp
  75% of sequence in fragments shorter than    8089 bp
  80% of sequence in fragments shorter than    9125 bp
  85% of sequence in fragments shorter than   10476 bp
  90% of sequence in fragments shorter than   12433 bp
  95% of sequence in fragments shorter than   16297 bp

# AgeI.PstI (ACCGGT+CTGCAG) double digest, 1374 thousand fragments (53386+1321469 sites), 2168 bp average fragment length
   5% of sequence in fragments shorter than     708 bp
  10% of sequence in fragments shorter than    1119 bp
  15% of sequence in fragments shorter than    1482 bp
  20% of sequence in fragments shorter than    1842 bp
  25% of sequence in fragments shorter than    2193 bp
  30% of sequence in fragments shorter than    2560 bp
  35% of sequence in fragments shorter than    2935 bp
  40% of sequence in fragments shorter than    3336 bp
  45% of sequence in fragments shorter than    3758 bp
  50% of sequence in fragments shorter than    4211 bp
  55% of sequence in fragments shorter than    4706 bp
  60% of sequence in fragments shorter than    5247 bp
  65% of sequence in fragments shorter than    5847 bp
  70% of sequence in fragments shorter than    6530 bp
  75% of sequence in fragments shorter than    7348 bp
  80% of sequence in fragments shorter than    8345 bp
  85% of sequence in fragments shorter than    9643 bp
  90% of sequence in fragments shorter than   11502 bp
  95% of sequence in fragments shorter than   15090 bp

# KpnI.PpuMI (GGTACC+RGGWCCY) double digest, 1372 thousand fragments (287974+1085138 sites), 2170 bp average fragment length
   5% of sequence in fragments shorter than     706 bp
  10% of sequence in fragments shorter than    1117 bp
  15% of sequence in fragments shorter than    1510 bp
  20% of sequence in fragments shorter than    1876 bp
  25% of sequence in fragments shorter than    2260 bp
  30% of sequence in fragments shorter than    2648 bp
  35% of sequence in fragments shorter than    3039 bp
  40% of sequence in fragments shorter than    3463 bp
  45% of sequence in fragments shorter than    3911 bp
  50% of sequence in fragments shorter than    4393 bp
  55% of sequence in fragments shorter than    4921 bp
  60% of sequence in fragments shorter than    5507 bp
  65% of sequence in fragments shorter than    6163 bp
  70% of sequence in fragments shorter than    6925 bp
  75% of sequence in fragments shorter than    7831 bp
  80% of sequence in fragments shorter than    8932 bp
  85% of sequence in fragments shorter than   10378 bp
  90% of sequence in fragments shorter than   12562 bp
  95% of sequence in fragments shorter than   17116 bp

# NheI.PpuMI (GCTAGC+RGGWCCY) double digest, 1372 thousand fragments (287384+1085138 sites), 2171 bp average fragment length
   5% of sequence in fragments shorter than     707 bp
  10% of sequence in fragments shorter than    1150 bp
  15% of sequence in fragments shorter than    1516 bp
  20% of sequence in fragments shorter than    1883 bp
  25% of sequence in fragments shorter than    2234 bp
  30% of sequence in fragments shorter than    2611 bp
  35% of sequence in fragments shorter than    3009 bp
  40% of sequence in fragments shorter than    3425 bp
  45% of sequence in fragments shorter than    3868 bp
  50% of sequence in fragments shorter than    4344 bp
  55% of sequence in fragments shorter than    4866 bp
  60% of sequence in fragments shorter than    5437 bp
  65% of sequence in fragments shorter than    6082 bp
  70% of sequence in fragments shorter than    6828 bp
  75% of sequence in fragments shorter than    7698 bp
  80% of sequence in fragments shorter than    8758 bp
  85% of sequence in fragments shorter than   10161 bp
  90% of sequence in fragments shorter than   12240 bp
  95% of sequence in fragments shorter than   16352 bp

# KpnI.PvuII (GGTACC+CAGCTG) double digest, 1371 thousand fragments (287974+1084260 sites), 2172 bp average fragment length
   5% of sequence in fragments shorter than     742 bp
  10% of sequence in fragments shorter than    1146 bp
  15% of sequence in fragments shorter than    1488 bp
  20% of sequence in fragments shorter than    1823 bp
  25% of sequence in fragments shorter than    2172 bp
  30% of sequence in fragments shorter than    2524 bp
  35% of sequence in fragments shorter than    2866 bp
  40% of sequence in fragments shorter than    3237 bp
  45% of sequence in fragments shorter than    3635 bp
  50% of sequence in fragments shorter than    4058 bp
  55% of sequence in fragments shorter than    4519 bp
  60% of sequence in fragments shorter than    5033 bp
  65% of sequence in fragments shorter than    5607 bp
  70% of sequence in fragments shorter than    6263 bp
  75% of sequence in fragments shorter than    7028 bp
  80% of sequence in fragments shorter than    7970 bp
  85% of sequence in fragments shorter than    9194 bp
  90% of sequence in fragments shorter than   11041 bp
  95% of sequence in fragments shorter than   14687 bp

# EcoRV.StuI (GATATC+AGGCCT) double digest, 1371 thousand fragments (446473+925729 sites), 2172 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1098 bp
  15% of sequence in fragments shorter than    1462 bp
  20% of sequence in fragments shorter than    1842 bp
  25% of sequence in fragments shorter than    2203 bp
  30% of sequence in fragments shorter than    2568 bp
  35% of sequence in fragments shorter than    2934 bp
  40% of sequence in fragments shorter than    3309 bp
  45% of sequence in fragments shorter than    3697 bp
  50% of sequence in fragments shorter than    4108 bp
  55% of sequence in fragments shorter than    4549 bp
  60% of sequence in fragments shorter than    5025 bp
  65% of sequence in fragments shorter than    5554 bp
  70% of sequence in fragments shorter than    6146 bp
  75% of sequence in fragments shorter than    6839 bp
  80% of sequence in fragments shorter than    7661 bp
  85% of sequence in fragments shorter than    8694 bp
  90% of sequence in fragments shorter than   10119 bp
  95% of sequence in fragments shorter than   12532 bp

# AvaI.BamHI (CYCGRG+GGATCC) double digest, 1371 thousand fragments (1005714+365999 sites), 2173 bp average fragment length
   5% of sequence in fragments shorter than     713 bp
  10% of sequence in fragments shorter than    1151 bp
  15% of sequence in fragments shorter than    1556 bp
  20% of sequence in fragments shorter than    1958 bp
  25% of sequence in fragments shorter than    2356 bp
  30% of sequence in fragments shorter than    2782 bp
  35% of sequence in fragments shorter than    3229 bp
  40% of sequence in fragments shorter than    3672 bp
  45% of sequence in fragments shorter than    4176 bp
  50% of sequence in fragments shorter than    4726 bp
  55% of sequence in fragments shorter than    5336 bp
  60% of sequence in fragments shorter than    6022 bp
  65% of sequence in fragments shorter than    6810 bp
  70% of sequence in fragments shorter than    7718 bp
  75% of sequence in fragments shorter than    8823 bp
  80% of sequence in fragments shorter than   10175 bp
  85% of sequence in fragments shorter than   11979 bp
  90% of sequence in fragments shorter than   14662 bp
  95% of sequence in fragments shorter than   19996 bp

# NheI.PvuII (GCTAGC+CAGCTG) double digest, 1371 thousand fragments (287384+1084260 sites), 2173 bp average fragment length
   5% of sequence in fragments shorter than     749 bp
  10% of sequence in fragments shorter than    1162 bp
  15% of sequence in fragments shorter than    1515 bp
  20% of sequence in fragments shorter than    1841 bp
  25% of sequence in fragments shorter than    2173 bp
  30% of sequence in fragments shorter than    2499 bp
  35% of sequence in fragments shorter than    2849 bp
  40% of sequence in fragments shorter than    3218 bp
  45% of sequence in fragments shorter than    3608 bp
  50% of sequence in fragments shorter than    4022 bp
  55% of sequence in fragments shorter than    4479 bp
  60% of sequence in fragments shorter than    4980 bp
  65% of sequence in fragments shorter than    5537 bp
  70% of sequence in fragments shorter than    6184 bp
  75% of sequence in fragments shorter than    6928 bp
  80% of sequence in fragments shorter than    7840 bp
  85% of sequence in fragments shorter than    9033 bp
  90% of sequence in fragments shorter than   10798 bp
  95% of sequence in fragments shorter than   14111 bp

# AvrII.BglII (CCTAGG+AGATCT) double digest, 1369 thousand fragments (594956+774732 sites), 2176 bp average fragment length
   5% of sequence in fragments shorter than     753 bp
  10% of sequence in fragments shorter than    1145 bp
  15% of sequence in fragments shorter than    1469 bp
  20% of sequence in fragments shorter than    1776 bp
  25% of sequence in fragments shorter than    2083 bp
  30% of sequence in fragments shorter than    2377 bp
  35% of sequence in fragments shorter than    2687 bp
  40% of sequence in fragments shorter than    3009 bp
  45% of sequence in fragments shorter than    3346 bp
  50% of sequence in fragments shorter than    3700 bp
  55% of sequence in fragments shorter than    4083 bp
  60% of sequence in fragments shorter than    4498 bp
  65% of sequence in fragments shorter than    4962 bp
  70% of sequence in fragments shorter than    5491 bp
  75% of sequence in fragments shorter than    6100 bp
  80% of sequence in fragments shorter than    6851 bp
  85% of sequence in fragments shorter than    7829 bp
  90% of sequence in fragments shorter than    9250 bp
  95% of sequence in fragments shorter than   12112 bp

# BclI.HaeII (TGATCA+RGCGCY) double digest, 1368 thousand fragments (738785+630101 sites), 2177 bp average fragment length
   5% of sequence in fragments shorter than     707 bp
  10% of sequence in fragments shorter than    1136 bp
  15% of sequence in fragments shorter than    1525 bp
  20% of sequence in fragments shorter than    1876 bp
  25% of sequence in fragments shorter than    2221 bp
  30% of sequence in fragments shorter than    2570 bp
  35% of sequence in fragments shorter than    2916 bp
  40% of sequence in fragments shorter than    3283 bp
  45% of sequence in fragments shorter than    3662 bp
  50% of sequence in fragments shorter than    4065 bp
  55% of sequence in fragments shorter than    4498 bp
  60% of sequence in fragments shorter than    4969 bp
  65% of sequence in fragments shorter than    5490 bp
  70% of sequence in fragments shorter than    6076 bp
  75% of sequence in fragments shorter than    6756 bp
  80% of sequence in fragments shorter than    7574 bp
  85% of sequence in fragments shorter than    8598 bp
  90% of sequence in fragments shorter than   10059 bp
  95% of sequence in fragments shorter than   12548 bp

# BspHI.HpaI (TCATGA+GTTAAC) double digest, 1367 thousand fragments (978290+389418 sites), 2179 bp average fragment length
   5% of sequence in fragments shorter than     763 bp
  10% of sequence in fragments shorter than    1158 bp
  15% of sequence in fragments shorter than    1498 bp
  20% of sequence in fragments shorter than    1808 bp
  25% of sequence in fragments shorter than    2101 bp
  30% of sequence in fragments shorter than    2407 bp
  35% of sequence in fragments shorter than    2723 bp
  40% of sequence in fragments shorter than    3047 bp
  45% of sequence in fragments shorter than    3388 bp
  50% of sequence in fragments shorter than    3754 bp
  55% of sequence in fragments shorter than    4148 bp
  60% of sequence in fragments shorter than    4576 bp
  65% of sequence in fragments shorter than    5058 bp
  70% of sequence in fragments shorter than    5608 bp
  75% of sequence in fragments shorter than    6254 bp
  80% of sequence in fragments shorter than    7054 bp
  85% of sequence in fragments shorter than    8108 bp
  90% of sequence in fragments shorter than    9681 bp
  95% of sequence in fragments shorter than   12967 bp

# FnuDII.HaeII (CGCG+RGCGCY) double digest, 1363 thousand fragments (733940+630101 sites), 2185 bp average fragment length
   5% of sequence in fragments shorter than     746 bp
  10% of sequence in fragments shorter than    1443 bp
  15% of sequence in fragments shorter than    2111 bp
  20% of sequence in fragments shorter than    2815 bp
  25% of sequence in fragments shorter than    3546 bp
  30% of sequence in fragments shorter than    4329 bp
  35% of sequence in fragments shorter than    5169 bp
  40% of sequence in fragments shorter than    6054 bp
  45% of sequence in fragments shorter than    7020 bp
  50% of sequence in fragments shorter than    8093 bp
  55% of sequence in fragments shorter than    9274 bp
  60% of sequence in fragments shorter than   10589 bp
  65% of sequence in fragments shorter than   12103 bp
  70% of sequence in fragments shorter than   13862 bp
  75% of sequence in fragments shorter than   15996 bp
  80% of sequence in fragments shorter than   18594 bp
  85% of sequence in fragments shorter than   21983 bp
  90% of sequence in fragments shorter than   26788 bp
  95% of sequence in fragments shorter than   34852 bp

# ApaI.NdeI (GGGCCC+CATATG) double digest, 1363 thousand fragments (460339+903360 sites), 2185 bp average fragment length
   5% of sequence in fragments shorter than     753 bp
  10% of sequence in fragments shorter than    1152 bp
  15% of sequence in fragments shorter than    1498 bp
  20% of sequence in fragments shorter than    1844 bp
  25% of sequence in fragments shorter than    2183 bp
  30% of sequence in fragments shorter than    2522 bp
  35% of sequence in fragments shorter than    2868 bp
  40% of sequence in fragments shorter than    3222 bp
  45% of sequence in fragments shorter than    3597 bp
  50% of sequence in fragments shorter than    3990 bp
  55% of sequence in fragments shorter than    4419 bp
  60% of sequence in fragments shorter than    4877 bp
  65% of sequence in fragments shorter than    5378 bp
  70% of sequence in fragments shorter than    5956 bp
  75% of sequence in fragments shorter than    6638 bp
  80% of sequence in fragments shorter than    7460 bp
  85% of sequence in fragments shorter than    8543 bp
  90% of sequence in fragments shorter than   10146 bp
  95% of sequence in fragments shorter than   13353 bp

# PmeI.PstI (GTTTAAAC+CTGCAG) double digest, 1363 thousand fragments (42094+1321469 sites), 2186 bp average fragment length
   5% of sequence in fragments shorter than     719 bp
  10% of sequence in fragments shorter than    1132 bp
  15% of sequence in fragments shorter than    1498 bp
  20% of sequence in fragments shorter than    1855 bp
  25% of sequence in fragments shorter than    2203 bp
  30% of sequence in fragments shorter than    2568 bp
  35% of sequence in fragments shorter than    2940 bp
  40% of sequence in fragments shorter than    3337 bp
  45% of sequence in fragments shorter than    3757 bp
  50% of sequence in fragments shorter than    4203 bp
  55% of sequence in fragments shorter than    4698 bp
  60% of sequence in fragments shorter than    5229 bp
  65% of sequence in fragments shorter than    5822 bp
  70% of sequence in fragments shorter than    6494 bp
  75% of sequence in fragments shorter than    7296 bp
  80% of sequence in fragments shorter than    8266 bp
  85% of sequence in fragments shorter than    9534 bp
  90% of sequence in fragments shorter than   11363 bp
  95% of sequence in fragments shorter than   14800 bp

# BscGI.SnaBI (CCCGT+TACGTA) double digest, 1362 thousand fragments (1231070+132299 sites), 2186 bp average fragment length
   5% of sequence in fragments shorter than     649 bp
  10% of sequence in fragments shorter than    1109 bp
  15% of sequence in fragments shorter than    1547 bp
  20% of sequence in fragments shorter than    1975 bp
  25% of sequence in fragments shorter than    2404 bp
  30% of sequence in fragments shorter than    2840 bp
  35% of sequence in fragments shorter than    3294 bp
  40% of sequence in fragments shorter than    3770 bp
  45% of sequence in fragments shorter than    4273 bp
  50% of sequence in fragments shorter than    4815 bp
  55% of sequence in fragments shorter than    5408 bp
  60% of sequence in fragments shorter than    6068 bp
  65% of sequence in fragments shorter than    6784 bp
  70% of sequence in fragments shorter than    7620 bp
  75% of sequence in fragments shorter than    8607 bp
  80% of sequence in fragments shorter than    9814 bp
  85% of sequence in fragments shorter than   11373 bp
  90% of sequence in fragments shorter than   13537 bp
  95% of sequence in fragments shorter than   17288 bp

# NcoI.SacI (CCATGG+GAGCTC) double digest, 1362 thousand fragments (759594+603323 sites), 2187 bp average fragment length
   5% of sequence in fragments shorter than     742 bp
  10% of sequence in fragments shorter than    1153 bp
  15% of sequence in fragments shorter than    1502 bp
  20% of sequence in fragments shorter than    1842 bp
  25% of sequence in fragments shorter than    2174 bp
  30% of sequence in fragments shorter than    2512 bp
  35% of sequence in fragments shorter than    2860 bp
  40% of sequence in fragments shorter than    3226 bp
  45% of sequence in fragments shorter than    3603 bp
  50% of sequence in fragments shorter than    3957 bp
  55% of sequence in fragments shorter than    4385 bp
  60% of sequence in fragments shorter than    4865 bp
  65% of sequence in fragments shorter than    5401 bp
  70% of sequence in fragments shorter than    6007 bp
  75% of sequence in fragments shorter than    6720 bp
  80% of sequence in fragments shorter than    7598 bp
  85% of sequence in fragments shorter than    8752 bp
  90% of sequence in fragments shorter than   10430 bp
  95% of sequence in fragments shorter than   13605 bp

# AflII.BsaAI (CTTAAG+YACGTR) double digest, 1356 thousand fragments (643284+713801 sites), 2196 bp average fragment length
   5% of sequence in fragments shorter than     790 bp
  10% of sequence in fragments shorter than    1177 bp
  15% of sequence in fragments shorter than    1498 bp
  20% of sequence in fragments shorter than    1819 bp
  25% of sequence in fragments shorter than    2110 bp
  30% of sequence in fragments shorter than    2424 bp
  35% of sequence in fragments shorter than    2743 bp
  40% of sequence in fragments shorter than    3054 bp
  45% of sequence in fragments shorter than    3396 bp
  50% of sequence in fragments shorter than    3751 bp
  55% of sequence in fragments shorter than    4138 bp
  60% of sequence in fragments shorter than    4557 bp
  65% of sequence in fragments shorter than    5016 bp
  70% of sequence in fragments shorter than    5537 bp
  75% of sequence in fragments shorter than    6137 bp
  80% of sequence in fragments shorter than    6865 bp
  85% of sequence in fragments shorter than    7800 bp
  90% of sequence in fragments shorter than    9142 bp
  95% of sequence in fragments shorter than   11604 bp

# BscGI.NaeI (CCCGT+GCCGGC) double digest, 1356 thousand fragments (1231070+125692 sites), 2197 bp average fragment length
   5% of sequence in fragments shorter than     626 bp
  10% of sequence in fragments shorter than    1111 bp
  15% of sequence in fragments shorter than    1586 bp
  20% of sequence in fragments shorter than    2061 bp
  25% of sequence in fragments shorter than    2543 bp
  30% of sequence in fragments shorter than    3039 bp
  35% of sequence in fragments shorter than    3563 bp
  40% of sequence in fragments shorter than    4115 bp
  45% of sequence in fragments shorter than    4703 bp
  50% of sequence in fragments shorter than    5350 bp
  55% of sequence in fragments shorter than    6056 bp
  60% of sequence in fragments shorter than    6830 bp
  65% of sequence in fragments shorter than    7716 bp
  70% of sequence in fragments shorter than    8742 bp
  75% of sequence in fragments shorter than    9950 bp
  80% of sequence in fragments shorter than   11427 bp
  85% of sequence in fragments shorter than   13312 bp
  90% of sequence in fragments shorter than   15999 bp
  95% of sequence in fragments shorter than   20619 bp

# ApaLI.HindIII (GTGCAC+AAGCTT) double digest, 1356 thousand fragments (496255+860365 sites), 2197 bp average fragment length
   5% of sequence in fragments shorter than     776 bp
  10% of sequence in fragments shorter than    1175 bp
  15% of sequence in fragments shorter than    1514 bp
  20% of sequence in fragments shorter than    1830 bp
  25% of sequence in fragments shorter than    2115 bp
  30% of sequence in fragments shorter than    2392 bp
  35% of sequence in fragments shorter than    2706 bp
  40% of sequence in fragments shorter than    3029 bp
  45% of sequence in fragments shorter than    3368 bp
  50% of sequence in fragments shorter than    3730 bp
  55% of sequence in fragments shorter than    4112 bp
  60% of sequence in fragments shorter than    4520 bp
  65% of sequence in fragments shorter than    4976 bp
  70% of sequence in fragments shorter than    5492 bp
  75% of sequence in fragments shorter than    6097 bp
  80% of sequence in fragments shorter than    6825 bp
  85% of sequence in fragments shorter than    7746 bp
  90% of sequence in fragments shorter than    9068 bp
  95% of sequence in fragments shorter than   11488 bp

# BspHI.SapI (TCATGA+GCTCTTC) double digest, 1355 thousand fragments (978290+377161 sites), 2199 bp average fragment length
   5% of sequence in fragments shorter than     779 bp
  10% of sequence in fragments shorter than    1175 bp
  15% of sequence in fragments shorter than    1519 bp
  20% of sequence in fragments shorter than    1844 bp
  25% of sequence in fragments shorter than    2118 bp
  30% of sequence in fragments shorter than    2423 bp
  35% of sequence in fragments shorter than    2738 bp
  40% of sequence in fragments shorter than    3054 bp
  45% of sequence in fragments shorter than    3394 bp
  50% of sequence in fragments shorter than    3756 bp
  55% of sequence in fragments shorter than    4140 bp
  60% of sequence in fragments shorter than    4558 bp
  65% of sequence in fragments shorter than    5028 bp
  70% of sequence in fragments shorter than    5561 bp
  75% of sequence in fragments shorter than    6187 bp
  80% of sequence in fragments shorter than    6940 bp
  85% of sequence in fragments shorter than    7930 bp
  90% of sequence in fragments shorter than    9362 bp
  95% of sequence in fragments shorter than   12158 bp

# BspHI.SmaI (TCATGA+CCCGGG) double digest, 1354 thousand fragments (978290+376940 sites), 2199 bp average fragment length
   5% of sequence in fragments shorter than     768 bp
  10% of sequence in fragments shorter than    1178 bp
  15% of sequence in fragments shorter than    1530 bp
  20% of sequence in fragments shorter than    1860 bp
  25% of sequence in fragments shorter than    2161 bp
  30% of sequence in fragments shorter than    2472 bp
  35% of sequence in fragments shorter than    2793 bp
  40% of sequence in fragments shorter than    3123 bp
  45% of sequence in fragments shorter than    3471 bp
  50% of sequence in fragments shorter than    3837 bp
  55% of sequence in fragments shorter than    4226 bp
  60% of sequence in fragments shorter than    4650 bp
  65% of sequence in fragments shorter than    5125 bp
  70% of sequence in fragments shorter than    5658 bp
  75% of sequence in fragments shorter than    6287 bp
  80% of sequence in fragments shorter than    7044 bp
  85% of sequence in fragments shorter than    8032 bp
  90% of sequence in fragments shorter than    9456 bp
  95% of sequence in fragments shorter than   12258 bp

# AvrII.NcoI (CCTAGG+CCATGG) double digest, 1354 thousand fragments (594956+759594 sites), 2200 bp average fragment length
   5% of sequence in fragments shorter than     744 bp
  10% of sequence in fragments shorter than    1136 bp
  15% of sequence in fragments shorter than    1488 bp
  20% of sequence in fragments shorter than    1835 bp
  25% of sequence in fragments shorter than    2175 bp
  30% of sequence in fragments shorter than    2514 bp
  35% of sequence in fragments shorter than    2858 bp
  40% of sequence in fragments shorter than    3205 bp
  45% of sequence in fragments shorter than    3580 bp
  50% of sequence in fragments shorter than    3972 bp
  55% of sequence in fragments shorter than    4388 bp
  60% of sequence in fragments shorter than    4845 bp
  65% of sequence in fragments shorter than    5362 bp
  70% of sequence in fragments shorter than    5946 bp
  75% of sequence in fragments shorter than    6640 bp
  80% of sequence in fragments shorter than    7508 bp
  85% of sequence in fragments shorter than    8623 bp
  90% of sequence in fragments shorter than   10291 bp
  95% of sequence in fragments shorter than   13502 bp

# PstI.SalI (CTGCAG+GTCGAC) double digest, 1352 thousand fragments (1321469+31396 sites), 2203 bp average fragment length
   5% of sequence in fragments shorter than     721 bp
  10% of sequence in fragments shorter than    1141 bp
  15% of sequence in fragments shorter than    1510 bp
  20% of sequence in fragments shorter than    1876 bp
  25% of sequence in fragments shorter than    2232 bp
  30% of sequence in fragments shorter than    2602 bp
  35% of sequence in fragments shorter than    2984 bp
  40% of sequence in fragments shorter than    3391 bp
  45% of sequence in fragments shorter than    3819 bp
  50% of sequence in fragments shorter than    4282 bp
  55% of sequence in fragments shorter than    4788 bp
  60% of sequence in fragments shorter than    5336 bp
  65% of sequence in fragments shorter than    5943 bp
  70% of sequence in fragments shorter than    6641 bp
  75% of sequence in fragments shorter than    7472 bp
  80% of sequence in fragments shorter than    8488 bp
  85% of sequence in fragments shorter than    9809 bp
  90% of sequence in fragments shorter than   11693 bp
  95% of sequence in fragments shorter than   15283 bp

# BscGI.XhoI (CCCGT+CTCGAG) double digest, 1351 thousand fragments (1231070+121308 sites), 2204 bp average fragment length
   5% of sequence in fragments shorter than     635 bp
  10% of sequence in fragments shorter than    1101 bp
  15% of sequence in fragments shorter than    1554 bp
  20% of sequence in fragments shorter than    2004 bp
  25% of sequence in fragments shorter than    2458 bp
  30% of sequence in fragments shorter than    2926 bp
  35% of sequence in fragments shorter than    3421 bp
  40% of sequence in fragments shorter than    3939 bp
  45% of sequence in fragments shorter than    4491 bp
  50% of sequence in fragments shorter than    5092 bp
  55% of sequence in fragments shorter than    5753 bp
  60% of sequence in fragments shorter than    6487 bp
  65% of sequence in fragments shorter than    7303 bp
  70% of sequence in fragments shorter than    8253 bp
  75% of sequence in fragments shorter than    9388 bp
  80% of sequence in fragments shorter than   10764 bp
  85% of sequence in fragments shorter than   12533 bp
  90% of sequence in fragments shorter than   15004 bp
  95% of sequence in fragments shorter than   19331 bp

# EcoRV.NdeI (GATATC+CATATG) double digest, 1349 thousand fragments (446473+903360 sites), 2208 bp average fragment length
   5% of sequence in fragments shorter than     742 bp
  10% of sequence in fragments shorter than    1140 bp
  15% of sequence in fragments shorter than    1472 bp
  20% of sequence in fragments shorter than    1818 bp
  25% of sequence in fragments shorter than    2163 bp
  30% of sequence in fragments shorter than    2512 bp
  35% of sequence in fragments shorter than    2869 bp
  40% of sequence in fragments shorter than    3242 bp
  45% of sequence in fragments shorter than    3636 bp
  50% of sequence in fragments shorter than    4057 bp
  55% of sequence in fragments shorter than    4514 bp
  60% of sequence in fragments shorter than    5021 bp
  65% of sequence in fragments shorter than    5590 bp
  70% of sequence in fragments shorter than    6256 bp
  75% of sequence in fragments shorter than    7055 bp
  80% of sequence in fragments shorter than    8077 bp
  85% of sequence in fragments shorter than    9456 bp
  90% of sequence in fragments shorter than   11597 bp
  95% of sequence in fragments shorter than   16115 bp

# AflII.BceAI (CTTAAG+ACGGC) double digest, 1348 thousand fragments (643284+705178 sites), 2210 bp average fragment length
   5% of sequence in fragments shorter than     761 bp
  10% of sequence in fragments shorter than    1179 bp
  15% of sequence in fragments shorter than    1547 bp
  20% of sequence in fragments shorter than    1892 bp
  25% of sequence in fragments shorter than    2227 bp
  30% of sequence in fragments shorter than    2563 bp
  35% of sequence in fragments shorter than    2909 bp
  40% of sequence in fragments shorter than    3263 bp
  45% of sequence in fragments shorter than    3617 bp
  50% of sequence in fragments shorter than    4001 bp
  55% of sequence in fragments shorter than    4419 bp
  60% of sequence in fragments shorter than    4876 bp
  65% of sequence in fragments shorter than    5390 bp
  70% of sequence in fragments shorter than    5970 bp
  75% of sequence in fragments shorter than    6652 bp
  80% of sequence in fragments shorter than    7489 bp
  85% of sequence in fragments shorter than    8548 bp
  90% of sequence in fragments shorter than   10109 bp
  95% of sequence in fragments shorter than   12997 bp

# ApaLI.XbaI (GTGCAC+TCTAGA) double digest, 1346 thousand fragments (496255+850998 sites), 2212 bp average fragment length
   5% of sequence in fragments shorter than     767 bp
  10% of sequence in fragments shorter than    1157 bp
  15% of sequence in fragments shorter than    1494 bp
  20% of sequence in fragments shorter than    1825 bp
  25% of sequence in fragments shorter than    2146 bp
  30% of sequence in fragments shorter than    2454 bp
  35% of sequence in fragments shorter than    2778 bp
  40% of sequence in fragments shorter than    3111 bp
  45% of sequence in fragments shorter than    3454 bp
  50% of sequence in fragments shorter than    3822 bp
  55% of sequence in fragments shorter than    4214 bp
  60% of sequence in fragments shorter than    4640 bp
  65% of sequence in fragments shorter than    5110 bp
  70% of sequence in fragments shorter than    5630 bp
  75% of sequence in fragments shorter than    6243 bp
  80% of sequence in fragments shorter than    6974 bp
  85% of sequence in fragments shorter than    7892 bp
  90% of sequence in fragments shorter than    9219 bp
  95% of sequence in fragments shorter than   11559 bp

# BglII.HgaI (AGATCT+GACGC) double digest, 1346 thousand fragments (774732+571889 sites), 2213 bp average fragment length
   5% of sequence in fragments shorter than     759 bp
  10% of sequence in fragments shorter than    1183 bp
  15% of sequence in fragments shorter than    1543 bp
  20% of sequence in fragments shorter than    1887 bp
  25% of sequence in fragments shorter than    2224 bp
  30% of sequence in fragments shorter than    2565 bp
  35% of sequence in fragments shorter than    2913 bp
  40% of sequence in fragments shorter than    3270 bp
  45% of sequence in fragments shorter than    3645 bp
  50% of sequence in fragments shorter than    4044 bp
  55% of sequence in fragments shorter than    4467 bp
  60% of sequence in fragments shorter than    4933 bp
  65% of sequence in fragments shorter than    5448 bp
  70% of sequence in fragments shorter than    6025 bp
  75% of sequence in fragments shorter than    6708 bp
  80% of sequence in fragments shorter than    7535 bp
  85% of sequence in fragments shorter than    8600 bp
  90% of sequence in fragments shorter than   10157 bp
  95% of sequence in fragments shorter than   13114 bp

# BglII.EciI (AGATCT+GGCGGA) double digest, 1345 thousand fragments (774732+571273 sites), 2214 bp average fragment length
   5% of sequence in fragments shorter than     759 bp
  10% of sequence in fragments shorter than    1170 bp
  15% of sequence in fragments shorter than    1524 bp
  20% of sequence in fragments shorter than    1852 bp
  25% of sequence in fragments shorter than    2183 bp
  30% of sequence in fragments shorter than    2517 bp
  35% of sequence in fragments shorter than    2858 bp
  40% of sequence in fragments shorter than    3212 bp
  45% of sequence in fragments shorter than    3582 bp
  50% of sequence in fragments shorter than    3975 bp
  55% of sequence in fragments shorter than    4394 bp
  60% of sequence in fragments shorter than    4859 bp
  65% of sequence in fragments shorter than    5374 bp
  70% of sequence in fragments shorter than    5958 bp
  75% of sequence in fragments shorter than    6654 bp
  80% of sequence in fragments shorter than    7495 bp
  85% of sequence in fragments shorter than    8589 bp
  90% of sequence in fragments shorter than   10239 bp
  95% of sequence in fragments shorter than   13538 bp

# BamHI.BspHI (GGATCC+TCATGA) double digest, 1343 thousand fragments (365999+978290 sites), 2217 bp average fragment length
   5% of sequence in fragments shorter than     773 bp
  10% of sequence in fragments shorter than    1186 bp
  15% of sequence in fragments shorter than    1528 bp
  20% of sequence in fragments shorter than    1857 bp
  25% of sequence in fragments shorter than    2165 bp
  30% of sequence in fragments shorter than    2475 bp
  35% of sequence in fragments shorter than    2795 bp
  40% of sequence in fragments shorter than    3116 bp
  45% of sequence in fragments shorter than    3463 bp
  50% of sequence in fragments shorter than    3827 bp
  55% of sequence in fragments shorter than    4214 bp
  60% of sequence in fragments shorter than    4637 bp
  65% of sequence in fragments shorter than    5101 bp
  70% of sequence in fragments shorter than    5631 bp
  75% of sequence in fragments shorter than    6245 bp
  80% of sequence in fragments shorter than    6986 bp
  85% of sequence in fragments shorter than    7954 bp
  90% of sequence in fragments shorter than    9352 bp
  95% of sequence in fragments shorter than   12014 bp

# BsaAI.HaeII (YACGTR+RGCGCY) double digest, 1343 thousand fragments (713801+630101 sites), 2218 bp average fragment length
   5% of sequence in fragments shorter than     695 bp
  10% of sequence in fragments shorter than    1165 bp
  15% of sequence in fragments shorter than    1595 bp
  20% of sequence in fragments shorter than    1996 bp
  25% of sequence in fragments shorter than    2402 bp
  30% of sequence in fragments shorter than    2813 bp
  35% of sequence in fragments shorter than    3231 bp
  40% of sequence in fragments shorter than    3650 bp
  45% of sequence in fragments shorter than    4102 bp
  50% of sequence in fragments shorter than    4580 bp
  55% of sequence in fragments shorter than    5106 bp
  60% of sequence in fragments shorter than    5680 bp
  65% of sequence in fragments shorter than    6328 bp
  70% of sequence in fragments shorter than    7061 bp
  75% of sequence in fragments shorter than    7926 bp
  80% of sequence in fragments shorter than    8963 bp
  85% of sequence in fragments shorter than   10303 bp
  90% of sequence in fragments shorter than   12197 bp
  95% of sequence in fragments shorter than   15430 bp

# ApaLI.EcoRI (GTGCAC+GAATTC) double digest, 1343 thousand fragments (496255+847401 sites), 2218 bp average fragment length
   5% of sequence in fragments shorter than     754 bp
  10% of sequence in fragments shorter than    1179 bp
  15% of sequence in fragments shorter than    1538 bp
  20% of sequence in fragments shorter than    1872 bp
  25% of sequence in fragments shorter than    2197 bp
  30% of sequence in fragments shorter than    2519 bp
  35% of sequence in fragments shorter than    2813 bp
  40% of sequence in fragments shorter than    3151 bp
  45% of sequence in fragments shorter than    3485 bp
  50% of sequence in fragments shorter than    3856 bp
  55% of sequence in fragments shorter than    4249 bp
  60% of sequence in fragments shorter than    4675 bp
  65% of sequence in fragments shorter than    5148 bp
  70% of sequence in fragments shorter than    5683 bp
  75% of sequence in fragments shorter than    6295 bp
  80% of sequence in fragments shorter than    7026 bp
  85% of sequence in fragments shorter than    7964 bp
  90% of sequence in fragments shorter than    9269 bp
  95% of sequence in fragments shorter than   11517 bp

# MluI.PstI (ACGCGT+CTGCAG) double digest, 1342 thousand fragments (21415+1321469 sites), 2219 bp average fragment length
   5% of sequence in fragments shorter than     726 bp
  10% of sequence in fragments shorter than    1147 bp
  15% of sequence in fragments shorter than    1521 bp
  20% of sequence in fragments shorter than    1890 bp
  25% of sequence in fragments shorter than    2247 bp
  30% of sequence in fragments shorter than    2624 bp
  35% of sequence in fragments shorter than    3010 bp
  40% of sequence in fragments shorter than    3423 bp
  45% of sequence in fragments shorter than    3858 bp
  50% of sequence in fragments shorter than    4327 bp
  55% of sequence in fragments shorter than    4838 bp
  60% of sequence in fragments shorter than    5394 bp
  65% of sequence in fragments shorter than    6008 bp
  70% of sequence in fragments shorter than    6721 bp
  75% of sequence in fragments shorter than    7561 bp
  80% of sequence in fragments shorter than    8588 bp
  85% of sequence in fragments shorter than    9928 bp
  90% of sequence in fragments shorter than   11838 bp
  95% of sequence in fragments shorter than   15530 bp

# BclI.SacI (TGATCA+GAGCTC) double digest, 1341 thousand fragments (738785+603323 sites), 2221 bp average fragment length
   5% of sequence in fragments shorter than     785 bp
  10% of sequence in fragments shorter than    1177 bp
  15% of sequence in fragments shorter than    1520 bp
  20% of sequence in fragments shorter than    1838 bp
  25% of sequence in fragments shorter than    2133 bp
  30% of sequence in fragments shorter than    2447 bp
  35% of sequence in fragments shorter than    2762 bp
  40% of sequence in fragments shorter than    3091 bp
  45% of sequence in fragments shorter than    3432 bp
  50% of sequence in fragments shorter than    3791 bp
  55% of sequence in fragments shorter than    4177 bp
  60% of sequence in fragments shorter than    4601 bp
  65% of sequence in fragments shorter than    5065 bp
  70% of sequence in fragments shorter than    5594 bp
  75% of sequence in fragments shorter than    6209 bp
  80% of sequence in fragments shorter than    6960 bp
  85% of sequence in fragments shorter than    7921 bp
  90% of sequence in fragments shorter than    9311 bp
  95% of sequence in fragments shorter than   11943 bp

# AvaI.BstEII (CYCGRG+GGTNACC) double digest, 1341 thousand fragments (1005714+335865 sites), 2221 bp average fragment length
   5% of sequence in fragments shorter than     722 bp
  10% of sequence in fragments shorter than    1170 bp
  15% of sequence in fragments shorter than    1591 bp
  20% of sequence in fragments shorter than    2006 bp
  25% of sequence in fragments shorter than    2433 bp
  30% of sequence in fragments shorter than    2880 bp
  35% of sequence in fragments shorter than    3350 bp
  40% of sequence in fragments shorter than    3856 bp
  45% of sequence in fragments shorter than    4397 bp
  50% of sequence in fragments shorter than    4981 bp
  55% of sequence in fragments shorter than    5620 bp
  60% of sequence in fragments shorter than    6335 bp
  65% of sequence in fragments shorter than    7147 bp
  70% of sequence in fragments shorter than    8085 bp
  75% of sequence in fragments shorter than    9209 bp
  80% of sequence in fragments shorter than   10621 bp
  85% of sequence in fragments shorter than   12492 bp
  90% of sequence in fragments shorter than   15314 bp
  95% of sequence in fragments shorter than   21197 bp

# AflII.BciVI (CTTAAG+GTATCC) double digest, 1338 thousand fragments (643284+696093 sites), 2225 bp average fragment length
   5% of sequence in fragments shorter than     794 bp
  10% of sequence in fragments shorter than    1198 bp
  15% of sequence in fragments shorter than    1548 bp
  20% of sequence in fragments shorter than    1863 bp
  25% of sequence in fragments shorter than    2167 bp
  30% of sequence in fragments shorter than    2482 bp
  35% of sequence in fragments shorter than    2800 bp
  40% of sequence in fragments shorter than    3131 bp
  45% of sequence in fragments shorter than    3483 bp
  50% of sequence in fragments shorter than    3851 bp
  55% of sequence in fragments shorter than    4242 bp
  60% of sequence in fragments shorter than    4673 bp
  65% of sequence in fragments shorter than    5151 bp
  70% of sequence in fragments shorter than    5698 bp
  75% of sequence in fragments shorter than    6344 bp
  80% of sequence in fragments shorter than    7130 bp
  85% of sequence in fragments shorter than    8168 bp
  90% of sequence in fragments shorter than    9675 bp
  95% of sequence in fragments shorter than   12624 bp

# BglII.MfeI (AGATCT+CAATTG) double digest, 1338 thousand fragments (774732+564303 sites), 2226 bp average fragment length
   5% of sequence in fragments shorter than     769 bp
  10% of sequence in fragments shorter than    1169 bp
  15% of sequence in fragments shorter than    1501 bp
  20% of sequence in fragments shorter than    1814 bp
  25% of sequence in fragments shorter than    2118 bp
  30% of sequence in fragments shorter than    2421 bp
  35% of sequence in fragments shorter than    2743 bp
  40% of sequence in fragments shorter than    3078 bp
  45% of sequence in fragments shorter than    3431 bp
  50% of sequence in fragments shorter than    3800 bp
  55% of sequence in fragments shorter than    4203 bp
  60% of sequence in fragments shorter than    4647 bp
  65% of sequence in fragments shorter than    5144 bp
  70% of sequence in fragments shorter than    5709 bp
  75% of sequence in fragments shorter than    6372 bp
  80% of sequence in fragments shorter than    7189 bp
  85% of sequence in fragments shorter than    8276 bp
  90% of sequence in fragments shorter than    9919 bp
  95% of sequence in fragments shorter than   13162 bp

# FnuDII.SacI (CGCG+GAGCTC) double digest, 1336 thousand fragments (733940+603323 sites), 2229 bp average fragment length
   5% of sequence in fragments shorter than     841 bp
  10% of sequence in fragments shorter than    1391 bp
  15% of sequence in fragments shorter than    1883 bp
  20% of sequence in fragments shorter than    2360 bp
  25% of sequence in fragments shorter than    2844 bp
  30% of sequence in fragments shorter than    3345 bp
  35% of sequence in fragments shorter than    3869 bp
  40% of sequence in fragments shorter than    4413 bp
  45% of sequence in fragments shorter than    4991 bp
  50% of sequence in fragments shorter than    5617 bp
  55% of sequence in fragments shorter than    6307 bp
  60% of sequence in fragments shorter than    7069 bp
  65% of sequence in fragments shorter than    7920 bp
  70% of sequence in fragments shorter than    8908 bp
  75% of sequence in fragments shorter than   10064 bp
  80% of sequence in fragments shorter than   11519 bp
  85% of sequence in fragments shorter than   13406 bp
  90% of sequence in fragments shorter than   16143 bp
  95% of sequence in fragments shorter than   21381 bp

# PstI.SgrAI (CTGCAG+CRCCGGYG) double digest, 1336 thousand fragments (1321469+15270 sites), 2230 bp average fragment length
   5% of sequence in fragments shorter than     729 bp
  10% of sequence in fragments shorter than    1154 bp
  15% of sequence in fragments shorter than    1530 bp
  20% of sequence in fragments shorter than    1900 bp
  25% of sequence in fragments shorter than    2261 bp
  30% of sequence in fragments shorter than    2641 bp
  35% of sequence in fragments shorter than    3030 bp
  40% of sequence in fragments shorter than    3444 bp
  45% of sequence in fragments shorter than    3883 bp
  50% of sequence in fragments shorter than    4355 bp
  55% of sequence in fragments shorter than    4870 bp
  60% of sequence in fragments shorter than    5430 bp
  65% of sequence in fragments shorter than    6049 bp
  70% of sequence in fragments shorter than    6766 bp
  75% of sequence in fragments shorter than    7611 bp
  80% of sequence in fragments shorter than    8647 bp
  85% of sequence in fragments shorter than    9993 bp
  90% of sequence in fragments shorter than   11936 bp
  95% of sequence in fragments shorter than   15650 bp

# AccI.BsrBI (GTMKAC+CCGCTC) double digest, 1336 thousand fragments (1101063+235523 sites), 2230 bp average fragment length
   5% of sequence in fragments shorter than     791 bp
  10% of sequence in fragments shorter than    1207 bp
  15% of sequence in fragments shorter than    1552 bp
  20% of sequence in fragments shorter than    1885 bp
  25% of sequence in fragments shorter than    2203 bp
  30% of sequence in fragments shorter than    2522 bp
  35% of sequence in fragments shorter than    2837 bp
  40% of sequence in fragments shorter than    3172 bp
  45% of sequence in fragments shorter than    3509 bp
  50% of sequence in fragments shorter than    3878 bp
  55% of sequence in fragments shorter than    4279 bp
  60% of sequence in fragments shorter than    4709 bp
  65% of sequence in fragments shorter than    5190 bp
  70% of sequence in fragments shorter than    5709 bp
  75% of sequence in fragments shorter than    6312 bp
  80% of sequence in fragments shorter than    7053 bp
  85% of sequence in fragments shorter than    8000 bp
  90% of sequence in fragments shorter than    9343 bp
  95% of sequence in fragments shorter than   11713 bp

# NruI.PstI (TCGCGA+CTGCAG) double digest, 1335 thousand fragments (14872+1321469 sites), 2230 bp average fragment length
   5% of sequence in fragments shorter than     729 bp
  10% of sequence in fragments shorter than    1153 bp
  15% of sequence in fragments shorter than    1529 bp
  20% of sequence in fragments shorter than    1897 bp
  25% of sequence in fragments shorter than    2256 bp
  30% of sequence in fragments shorter than    2635 bp
  35% of sequence in fragments shorter than    3022 bp
  40% of sequence in fragments shorter than    3436 bp
  45% of sequence in fragments shorter than    3872 bp
  50% of sequence in fragments shorter than    4343 bp
  55% of sequence in fragments shorter than    4854 bp
  60% of sequence in fragments shorter than    5411 bp
  65% of sequence in fragments shorter than    6027 bp
  70% of sequence in fragments shorter than    6741 bp
  75% of sequence in fragments shorter than    7584 bp
  80% of sequence in fragments shorter than    8613 bp
  85% of sequence in fragments shorter than    9952 bp
  90% of sequence in fragments shorter than   11889 bp
  95% of sequence in fragments shorter than   15578 bp

# PstI.PvuI (CTGCAG+CGATCG) double digest, 1335 thousand fragments (1321469+14186 sites), 2231 bp average fragment length
   5% of sequence in fragments shorter than     731 bp
  10% of sequence in fragments shorter than    1156 bp
  15% of sequence in fragments shorter than    1531 bp
  20% of sequence in fragments shorter than    1897 bp
  25% of sequence in fragments shorter than    2255 bp
  30% of sequence in fragments shorter than    2634 bp
  35% of sequence in fragments shorter than    3021 bp
  40% of sequence in fragments shorter than    3435 bp
  45% of sequence in fragments shorter than    3870 bp
  50% of sequence in fragments shorter than    4339 bp
  55% of sequence in fragments shorter than    4851 bp
  60% of sequence in fragments shorter than    5408 bp
  65% of sequence in fragments shorter than    6023 bp
  70% of sequence in fragments shorter than    6735 bp
  75% of sequence in fragments shorter than    7576 bp
  80% of sequence in fragments shorter than    8604 bp
  85% of sequence in fragments shorter than    9936 bp
  90% of sequence in fragments shorter than   11839 bp
  95% of sequence in fragments shorter than   15454 bp

# BceAI.HaeII (ACGGC+RGCGCY) double digest, 1334 thousand fragments (705178+630101 sites), 2232 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1183 bp
  15% of sequence in fragments shorter than    1682 bp
  20% of sequence in fragments shorter than    2167 bp
  25% of sequence in fragments shorter than    2677 bp
  30% of sequence in fragments shorter than    3203 bp
  35% of sequence in fragments shorter than    3749 bp
  40% of sequence in fragments shorter than    4331 bp
  45% of sequence in fragments shorter than    4950 bp
  50% of sequence in fragments shorter than    5623 bp
  55% of sequence in fragments shorter than    6352 bp
  60% of sequence in fragments shorter than    7164 bp
  65% of sequence in fragments shorter than    8080 bp
  70% of sequence in fragments shorter than    9119 bp
  75% of sequence in fragments shorter than   10363 bp
  80% of sequence in fragments shorter than   11894 bp
  85% of sequence in fragments shorter than   13846 bp
  90% of sequence in fragments shorter than   16630 bp
  95% of sequence in fragments shorter than   21516 bp

# FseI.PstI (GGCCGGCC+CTGCAG) double digest, 1334 thousand fragments (13662+1321469 sites), 2232 bp average fragment length
   5% of sequence in fragments shorter than     729 bp
  10% of sequence in fragments shorter than    1155 bp
  15% of sequence in fragments shorter than    1532 bp
  20% of sequence in fragments shorter than    1902 bp
  25% of sequence in fragments shorter than    2263 bp
  30% of sequence in fragments shorter than    2644 bp
  35% of sequence in fragments shorter than    3033 bp
  40% of sequence in fragments shorter than    3448 bp
  45% of sequence in fragments shorter than    3888 bp
  50% of sequence in fragments shorter than    4362 bp
  55% of sequence in fragments shorter than    4876 bp
  60% of sequence in fragments shorter than    5438 bp
  65% of sequence in fragments shorter than    6058 bp
  70% of sequence in fragments shorter than    6777 bp
  75% of sequence in fragments shorter than    7622 bp
  80% of sequence in fragments shorter than    8663 bp
  85% of sequence in fragments shorter than   10008 bp
  90% of sequence in fragments shorter than   11962 bp
  95% of sequence in fragments shorter than   15700 bp

# AvrII.BclI (CCTAGG+TGATCA) double digest, 1333 thousand fragments (594956+738785 sites), 2234 bp average fragment length
   5% of sequence in fragments shorter than     762 bp
  10% of sequence in fragments shorter than    1172 bp
  15% of sequence in fragments shorter than    1522 bp
  20% of sequence in fragments shorter than    1848 bp
  25% of sequence in fragments shorter than    2167 bp
  30% of sequence in fragments shorter than    2477 bp
  35% of sequence in fragments shorter than    2794 bp
  40% of sequence in fragments shorter than    3120 bp
  45% of sequence in fragments shorter than    3465 bp
  50% of sequence in fragments shorter than    3832 bp
  55% of sequence in fragments shorter than    4220 bp
  60% of sequence in fragments shorter than    4640 bp
  65% of sequence in fragments shorter than    5106 bp
  70% of sequence in fragments shorter than    5633 bp
  75% of sequence in fragments shorter than    6265 bp
  80% of sequence in fragments shorter than    7025 bp
  85% of sequence in fragments shorter than    8011 bp
  90% of sequence in fragments shorter than    9449 bp
  95% of sequence in fragments shorter than   12301 bp

# AvaIII.SpeI (ATGCAT+ACTAGT) double digest, 1333 thousand fragments (933357+400286 sites), 2235 bp average fragment length
   5% of sequence in fragments shorter than     786 bp
  10% of sequence in fragments shorter than    1194 bp
  15% of sequence in fragments shorter than    1547 bp
  20% of sequence in fragments shorter than    1883 bp
  25% of sequence in fragments shorter than    2199 bp
  30% of sequence in fragments shorter than    2530 bp
  35% of sequence in fragments shorter than    2860 bp
  40% of sequence in fragments shorter than    3216 bp
  45% of sequence in fragments shorter than    3582 bp
  50% of sequence in fragments shorter than    3979 bp
  55% of sequence in fragments shorter than    4410 bp
  60% of sequence in fragments shorter than    4888 bp
  65% of sequence in fragments shorter than    5431 bp
  70% of sequence in fragments shorter than    6038 bp
  75% of sequence in fragments shorter than    6759 bp
  80% of sequence in fragments shorter than    7655 bp
  85% of sequence in fragments shorter than    8849 bp
  90% of sequence in fragments shorter than   10632 bp
  95% of sequence in fragments shorter than   14298 bp

# HgaI.NcoI (GACGC+CCATGG) double digest, 1331 thousand fragments (571889+759594 sites), 2238 bp average fragment length
   5% of sequence in fragments shorter than     749 bp
  10% of sequence in fragments shorter than    1191 bp
  15% of sequence in fragments shorter than    1594 bp
  20% of sequence in fragments shorter than    1979 bp
  25% of sequence in fragments shorter than    2357 bp
  30% of sequence in fragments shorter than    2729 bp
  35% of sequence in fragments shorter than    3115 bp
  40% of sequence in fragments shorter than    3532 bp
  45% of sequence in fragments shorter than    3975 bp
  50% of sequence in fragments shorter than    4441 bp
  55% of sequence in fragments shorter than    4933 bp
  60% of sequence in fragments shorter than    5483 bp
  65% of sequence in fragments shorter than    6075 bp
  70% of sequence in fragments shorter than    6773 bp
  75% of sequence in fragments shorter than    7610 bp
  80% of sequence in fragments shorter than    8636 bp
  85% of sequence in fragments shorter than    9948 bp
  90% of sequence in fragments shorter than   11878 bp
  95% of sequence in fragments shorter than   15538 bp

# AccI.KasI (GTMKAC+GGCGCC) double digest, 1330 thousand fragments (1101063+230381 sites), 2238 bp average fragment length
   5% of sequence in fragments shorter than     786 bp
  10% of sequence in fragments shorter than    1201 bp
  15% of sequence in fragments shorter than    1545 bp
  20% of sequence in fragments shorter than    1877 bp
  25% of sequence in fragments shorter than    2198 bp
  30% of sequence in fragments shorter than    2518 bp
  35% of sequence in fragments shorter than    2838 bp
  40% of sequence in fragments shorter than    3177 bp
  45% of sequence in fragments shorter than    3529 bp
  50% of sequence in fragments shorter than    3900 bp
  55% of sequence in fragments shorter than    4299 bp
  60% of sequence in fragments shorter than    4724 bp
  65% of sequence in fragments shorter than    5204 bp
  70% of sequence in fragments shorter than    5694 bp
  75% of sequence in fragments shorter than    6291 bp
  80% of sequence in fragments shorter than    7035 bp
  85% of sequence in fragments shorter than    7994 bp
  90% of sequence in fragments shorter than    9339 bp
  95% of sequence in fragments shorter than   11793 bp

# NotI.PstI (GCGGCCGC+CTGCAG) double digest, 1330 thousand fragments (9795+1321469 sites), 2239 bp average fragment length
   5% of sequence in fragments shorter than     731 bp
  10% of sequence in fragments shorter than    1159 bp
  15% of sequence in fragments shorter than    1536 bp
  20% of sequence in fragments shorter than    1907 bp
  25% of sequence in fragments shorter than    2269 bp
  30% of sequence in fragments shorter than    2651 bp
  35% of sequence in fragments shorter than    3042 bp
  40% of sequence in fragments shorter than    3457 bp
  45% of sequence in fragments shorter than    3897 bp
  50% of sequence in fragments shorter than    4372 bp
  55% of sequence in fragments shorter than    4887 bp
  60% of sequence in fragments shorter than    5448 bp
  65% of sequence in fragments shorter than    6070 bp
  70% of sequence in fragments shorter than    6791 bp
  75% of sequence in fragments shorter than    7637 bp
  80% of sequence in fragments shorter than    8678 bp
  85% of sequence in fragments shorter than   10026 bp
  90% of sequence in fragments shorter than   11980 bp
  95% of sequence in fragments shorter than   15710 bp

# PstI.RsrII (CTGCAG+CGGWCCG) double digest, 1330 thousand fragments (1321469+9764 sites), 2239 bp average fragment length
   5% of sequence in fragments shorter than     732 bp
  10% of sequence in fragments shorter than    1159 bp
  15% of sequence in fragments shorter than    1535 bp
  20% of sequence in fragments shorter than    1906 bp
  25% of sequence in fragments shorter than    2267 bp
  30% of sequence in fragments shorter than    2649 bp
  35% of sequence in fragments shorter than    3038 bp
  40% of sequence in fragments shorter than    3452 bp
  45% of sequence in fragments shorter than    3892 bp
  50% of sequence in fragments shorter than    4365 bp
  55% of sequence in fragments shorter than    4880 bp
  60% of sequence in fragments shorter than    5441 bp
  65% of sequence in fragments shorter than    6062 bp
  70% of sequence in fragments shorter than    6780 bp
  75% of sequence in fragments shorter than    7624 bp
  80% of sequence in fragments shorter than    8663 bp
  85% of sequence in fragments shorter than   10011 bp
  90% of sequence in fragments shorter than   11961 bp
  95% of sequence in fragments shorter than   15697 bp

# EciI.NcoI (GGCGGA+CCATGG) double digest, 1330 thousand fragments (571273+759594 sites), 2239 bp average fragment length
   5% of sequence in fragments shorter than     750 bp
  10% of sequence in fragments shorter than    1175 bp
  15% of sequence in fragments shorter than    1542 bp
  20% of sequence in fragments shorter than    1903 bp
  25% of sequence in fragments shorter than    2259 bp
  30% of sequence in fragments shorter than    2623 bp
  35% of sequence in fragments shorter than    2992 bp
  40% of sequence in fragments shorter than    3389 bp
  45% of sequence in fragments shorter than    3809 bp
  50% of sequence in fragments shorter than    4260 bp
  55% of sequence in fragments shorter than    4729 bp
  60% of sequence in fragments shorter than    5261 bp
  65% of sequence in fragments shorter than    5840 bp
  70% of sequence in fragments shorter than    6513 bp
  75% of sequence in fragments shorter than    7331 bp
  80% of sequence in fragments shorter than    8325 bp
  85% of sequence in fragments shorter than    9630 bp
  90% of sequence in fragments shorter than   11592 bp
  95% of sequence in fragments shorter than   15353 bp

# AvrII.FnuDII (CCTAGG+CGCG) double digest, 1328 thousand fragments (594956+733940 sites), 2243 bp average fragment length
   5% of sequence in fragments shorter than     835 bp
  10% of sequence in fragments shorter than    1405 bp
  15% of sequence in fragments shorter than    1903 bp
  20% of sequence in fragments shorter than    2377 bp
  25% of sequence in fragments shorter than    2847 bp
  30% of sequence in fragments shorter than    3326 bp
  35% of sequence in fragments shorter than    3810 bp
  40% of sequence in fragments shorter than    4298 bp
  45% of sequence in fragments shorter than    4836 bp
  50% of sequence in fragments shorter than    5417 bp
  55% of sequence in fragments shorter than    6044 bp
  60% of sequence in fragments shorter than    6735 bp
  65% of sequence in fragments shorter than    7524 bp
  70% of sequence in fragments shorter than    8419 bp
  75% of sequence in fragments shorter than    9477 bp
  80% of sequence in fragments shorter than   10773 bp
  85% of sequence in fragments shorter than   12464 bp
  90% of sequence in fragments shorter than   15032 bp
  95% of sequence in fragments shorter than   20058 bp

# AccI.SwaI (GTMKAC+ATTTAAAT) double digest, 1327 thousand fragments (1101063+226602 sites), 2245 bp average fragment length
   5% of sequence in fragments shorter than     787 bp
  10% of sequence in fragments shorter than    1196 bp
  15% of sequence in fragments shorter than    1539 bp
  20% of sequence in fragments shorter than    1872 bp
  25% of sequence in fragments shorter than    2196 bp
  30% of sequence in fragments shorter than    2523 bp
  35% of sequence in fragments shorter than    2852 bp
  40% of sequence in fragments shorter than    3200 bp
  45% of sequence in fragments shorter than    3563 bp
  50% of sequence in fragments shorter than    3951 bp
  55% of sequence in fragments shorter than    4370 bp
  60% of sequence in fragments shorter than    4828 bp
  65% of sequence in fragments shorter than    5341 bp
  70% of sequence in fragments shorter than    5906 bp
  75% of sequence in fragments shorter than    6538 bp
  80% of sequence in fragments shorter than    7373 bp
  85% of sequence in fragments shorter than    8461 bp
  90% of sequence in fragments shorter than   10047 bp
  95% of sequence in fragments shorter than   13063 bp

# AscI.PstI (GGCGCGCC+CTGCAG) double digest, 1325 thousand fragments (4751+1321469 sites), 2247 bp average fragment length
   5% of sequence in fragments shorter than     735 bp
  10% of sequence in fragments shorter than    1164 bp
  15% of sequence in fragments shorter than    1541 bp
  20% of sequence in fragments shorter than    1912 bp
  25% of sequence in fragments shorter than    2275 bp
  30% of sequence in fragments shorter than    2658 bp
  35% of sequence in fragments shorter than    3048 bp
  40% of sequence in fragments shorter than    3463 bp
  45% of sequence in fragments shorter than    3904 bp
  50% of sequence in fragments shorter than    4380 bp
  55% of sequence in fragments shorter than    4894 bp
  60% of sequence in fragments shorter than    5456 bp
  65% of sequence in fragments shorter than    6078 bp
  70% of sequence in fragments shorter than    6800 bp
  75% of sequence in fragments shorter than    7645 bp
  80% of sequence in fragments shorter than    8687 bp
  85% of sequence in fragments shorter than   10035 bp
  90% of sequence in fragments shorter than   11987 bp
  95% of sequence in fragments shorter than   15719 bp

# BciVI.HaeII (GTATCC+RGCGCY) double digest, 1325 thousand fragments (696093+630101 sites), 2247 bp average fragment length
   5% of sequence in fragments shorter than     725 bp
  10% of sequence in fragments shorter than    1189 bp
  15% of sequence in fragments shorter than    1590 bp
  20% of sequence in fragments shorter than    1952 bp
  25% of sequence in fragments shorter than    2316 bp
  30% of sequence in fragments shorter than    2692 bp
  35% of sequence in fragments shorter than    3072 bp
  40% of sequence in fragments shorter than    3457 bp
  45% of sequence in fragments shorter than    3861 bp
  50% of sequence in fragments shorter than    4286 bp
  55% of sequence in fragments shorter than    4745 bp
  60% of sequence in fragments shorter than    5248 bp
  65% of sequence in fragments shorter than    5802 bp
  70% of sequence in fragments shorter than    6425 bp
  75% of sequence in fragments shorter than    7145 bp
  80% of sequence in fragments shorter than    8024 bp
  85% of sequence in fragments shorter than    9119 bp
  90% of sequence in fragments shorter than   10622 bp
  95% of sequence in fragments shorter than   13225 bp

# SpeI.StuI (ACTAGT+AGGCCT) double digest, 1325 thousand fragments (400286+925729 sites), 2248 bp average fragment length
   5% of sequence in fragments shorter than     706 bp
  10% of sequence in fragments shorter than    1168 bp
  15% of sequence in fragments shorter than    1543 bp
  20% of sequence in fragments shorter than    1901 bp
  25% of sequence in fragments shorter than    2249 bp
  30% of sequence in fragments shorter than    2619 bp
  35% of sequence in fragments shorter than    2993 bp
  40% of sequence in fragments shorter than    3375 bp
  45% of sequence in fragments shorter than    3754 bp
  50% of sequence in fragments shorter than    4173 bp
  55% of sequence in fragments shorter than    4623 bp
  60% of sequence in fragments shorter than    5109 bp
  65% of sequence in fragments shorter than    5645 bp
  70% of sequence in fragments shorter than    6250 bp
  75% of sequence in fragments shorter than    6956 bp
  80% of sequence in fragments shorter than    7804 bp
  85% of sequence in fragments shorter than    8862 bp
  90% of sequence in fragments shorter than   10332 bp
  95% of sequence in fragments shorter than   12811 bp

# BglII.SphI (AGATCT+GCATGC) double digest, 1325 thousand fragments (774732+550952 sites), 2248 bp average fragment length
   5% of sequence in fragments shorter than     784 bp
  10% of sequence in fragments shorter than    1185 bp
  15% of sequence in fragments shorter than    1527 bp
  20% of sequence in fragments shorter than    1838 bp
  25% of sequence in fragments shorter than    2149 bp
  30% of sequence in fragments shorter than    2463 bp
  35% of sequence in fragments shorter than    2781 bp
  40% of sequence in fragments shorter than    3113 bp
  45% of sequence in fragments shorter than    3457 bp
  50% of sequence in fragments shorter than    3821 bp
  55% of sequence in fragments shorter than    4210 bp
  60% of sequence in fragments shorter than    4631 bp
  65% of sequence in fragments shorter than    5094 bp
  70% of sequence in fragments shorter than    5620 bp
  75% of sequence in fragments shorter than    6229 bp
  80% of sequence in fragments shorter than    6975 bp
  85% of sequence in fragments shorter than    7946 bp
  90% of sequence in fragments shorter than    9349 bp
  95% of sequence in fragments shorter than   12126 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# MfeI.NcoI (CAATTG+CCATGG) double digest, 1323 thousand fragments (564303+759594 sites), 2251 bp average fragment length
   5% of sequence in fragments shorter than     786 bp
  10% of sequence in fragments shorter than    1191 bp
  15% of sequence in fragments shorter than    1509 bp
  20% of sequence in fragments shorter than    1846 bp
  25% of sequence in fragments shorter than    2164 bp
  30% of sequence in fragments shorter than    2490 bp
  35% of sequence in fragments shorter than    2819 bp
  40% of sequence in fragments shorter than    3153 bp
  45% of sequence in fragments shorter than    3508 bp
  50% of sequence in fragments shorter than    3888 bp
  55% of sequence in fragments shorter than    4285 bp
  60% of sequence in fragments shorter than    4714 bp
  65% of sequence in fragments shorter than    5198 bp
  70% of sequence in fragments shorter than    5742 bp
  75% of sequence in fragments shorter than    6385 bp
  80% of sequence in fragments shorter than    7169 bp
  85% of sequence in fragments shorter than    8176 bp
  90% of sequence in fragments shorter than    9629 bp
  95% of sequence in fragments shorter than   12385 bp

# AvaIII.HpaI (ATGCAT+GTTAAC) double digest, 1322 thousand fragments (933357+389418 sites), 2253 bp average fragment length
   5% of sequence in fragments shorter than     790 bp
  10% of sequence in fragments shorter than    1204 bp
  15% of sequence in fragments shorter than    1565 bp
  20% of sequence in fragments shorter than    1908 bp
  25% of sequence in fragments shorter than    2236 bp
  30% of sequence in fragments shorter than    2575 bp
  35% of sequence in fragments shorter than    2914 bp
  40% of sequence in fragments shorter than    3275 bp
  45% of sequence in fragments shorter than    3644 bp
  50% of sequence in fragments shorter than    4045 bp
  55% of sequence in fragments shorter than    4478 bp
  60% of sequence in fragments shorter than    4954 bp
  65% of sequence in fragments shorter than    5491 bp
  70% of sequence in fragments shorter than    6113 bp
  75% of sequence in fragments shorter than    6863 bp
  80% of sequence in fragments shorter than    7783 bp
  85% of sequence in fragments shorter than    8993 bp
  90% of sequence in fragments shorter than   10848 bp
  95% of sequence in fragments shorter than   14632 bp

# ApaI.HindIII (GGGCCC+AAGCTT) double digest, 1320 thousand fragments (460339+860365 sites), 2257 bp average fragment length
   5% of sequence in fragments shorter than     769 bp
  10% of sequence in fragments shorter than    1196 bp
  15% of sequence in fragments shorter than    1569 bp
  20% of sequence in fragments shorter than    1913 bp
  25% of sequence in fragments shorter than    2238 bp
  30% of sequence in fragments shorter than    2586 bp
  35% of sequence in fragments shorter than    2934 bp
  40% of sequence in fragments shorter than    3295 bp
  45% of sequence in fragments shorter than    3676 bp
  50% of sequence in fragments shorter than    4081 bp
  55% of sequence in fragments shorter than    4516 bp
  60% of sequence in fragments shorter than    4990 bp
  65% of sequence in fragments shorter than    5518 bp
  70% of sequence in fragments shorter than    6114 bp
  75% of sequence in fragments shorter than    6816 bp
  80% of sequence in fragments shorter than    7660 bp
  85% of sequence in fragments shorter than    8765 bp
  90% of sequence in fragments shorter than   10344 bp
  95% of sequence in fragments shorter than   13276 bp

# BsrBI.PpuMI (CCGCTC+RGGWCCY) double digest, 1320 thousand fragments (235523+1085138 sites), 2257 bp average fragment length
   5% of sequence in fragments shorter than     720 bp
  10% of sequence in fragments shorter than    1196 bp
  15% of sequence in fragments shorter than    1624 bp
  20% of sequence in fragments shorter than    2040 bp
  25% of sequence in fragments shorter than    2477 bp
  30% of sequence in fragments shorter than    2930 bp
  35% of sequence in fragments shorter than    3386 bp
  40% of sequence in fragments shorter than    3849 bp
  45% of sequence in fragments shorter than    4365 bp
  50% of sequence in fragments shorter than    4923 bp
  55% of sequence in fragments shorter than    5538 bp
  60% of sequence in fragments shorter than    6212 bp
  65% of sequence in fragments shorter than    6980 bp
  70% of sequence in fragments shorter than    7852 bp
  75% of sequence in fragments shorter than    8875 bp
  80% of sequence in fragments shorter than   10157 bp
  85% of sequence in fragments shorter than   11812 bp
  90% of sequence in fragments shorter than   14275 bp
  95% of sequence in fragments shorter than   19047 bp

# BsrBI.PvuII (CCGCTC+CAGCTG) double digest, 1319 thousand fragments (235523+1084260 sites), 2258 bp average fragment length
   5% of sequence in fragments shorter than     745 bp
  10% of sequence in fragments shorter than    1184 bp
  15% of sequence in fragments shorter than    1583 bp
  20% of sequence in fragments shorter than    1969 bp
  25% of sequence in fragments shorter than    2362 bp
  30% of sequence in fragments shorter than    2760 bp
  35% of sequence in fragments shorter than    3177 bp
  40% of sequence in fragments shorter than    3614 bp
  45% of sequence in fragments shorter than    4084 bp
  50% of sequence in fragments shorter than    4589 bp
  55% of sequence in fragments shorter than    5142 bp
  60% of sequence in fragments shorter than    5746 bp
  65% of sequence in fragments shorter than    6420 bp
  70% of sequence in fragments shorter than    7191 bp
  75% of sequence in fragments shorter than    8099 bp
  80% of sequence in fragments shorter than    9214 bp
  85% of sequence in fragments shorter than   10682 bp
  90% of sequence in fragments shorter than   12837 bp
  95% of sequence in fragments shorter than   16935 bp

# BglII.ScaI (AGATCT+AGTACT) double digest, 1318 thousand fragments (774732+543793 sites), 2260 bp average fragment length
   5% of sequence in fragments shorter than     784 bp
  10% of sequence in fragments shorter than    1186 bp
  15% of sequence in fragments shorter than    1528 bp
  20% of sequence in fragments shorter than    1845 bp
  25% of sequence in fragments shorter than    2162 bp
  30% of sequence in fragments shorter than    2480 bp
  35% of sequence in fragments shorter than    2808 bp
  40% of sequence in fragments shorter than    3154 bp
  45% of sequence in fragments shorter than    3508 bp
  50% of sequence in fragments shorter than    3882 bp
  55% of sequence in fragments shorter than    4288 bp
  60% of sequence in fragments shorter than    4731 bp
  65% of sequence in fragments shorter than    5219 bp
  70% of sequence in fragments shorter than    5777 bp
  75% of sequence in fragments shorter than    6444 bp
  80% of sequence in fragments shorter than    7272 bp
  85% of sequence in fragments shorter than    8354 bp
  90% of sequence in fragments shorter than    9972 bp
  95% of sequence in fragments shorter than   13416 bp

# BscGI.ClaI (CCCGT+ATCGAT) double digest, 1317 thousand fragments (1231070+86477 sites), 2262 bp average fragment length
   5% of sequence in fragments shorter than     667 bp
  10% of sequence in fragments shorter than    1145 bp
  15% of sequence in fragments shorter than    1600 bp
  20% of sequence in fragments shorter than    2049 bp
  25% of sequence in fragments shorter than    2496 bp
  30% of sequence in fragments shorter than    2959 bp
  35% of sequence in fragments shorter than    3439 bp
  40% of sequence in fragments shorter than    3944 bp
  45% of sequence in fragments shorter than    4481 bp
  50% of sequence in fragments shorter than    5063 bp
  55% of sequence in fragments shorter than    5704 bp
  60% of sequence in fragments shorter than    6404 bp
  65% of sequence in fragments shorter than    7190 bp
  70% of sequence in fragments shorter than    8096 bp
  75% of sequence in fragments shorter than    9174 bp
  80% of sequence in fragments shorter than   10472 bp
  85% of sequence in fragments shorter than   12165 bp
  90% of sequence in fragments shorter than   14537 bp
  95% of sequence in fragments shorter than   18542 bp

# BsaAI.SacI (YACGTR+GAGCTC) double digest, 1316 thousand fragments (713801+603323 sites), 2263 bp average fragment length
   5% of sequence in fragments shorter than     789 bp
  10% of sequence in fragments shorter than    1201 bp
  15% of sequence in fragments shorter than    1560 bp
  20% of sequence in fragments shorter than    1897 bp
  25% of sequence in fragments shorter than    2236 bp
  30% of sequence in fragments shorter than    2577 bp
  35% of sequence in fragments shorter than    2921 bp
  40% of sequence in fragments shorter than    3277 bp
  45% of sequence in fragments shorter than    3636 bp
  50% of sequence in fragments shorter than    4020 bp
  55% of sequence in fragments shorter than    4444 bp
  60% of sequence in fragments shorter than    4915 bp
  65% of sequence in fragments shorter than    5436 bp
  70% of sequence in fragments shorter than    6026 bp
  75% of sequence in fragments shorter than    6714 bp
  80% of sequence in fragments shorter than    7551 bp
  85% of sequence in fragments shorter than    8642 bp
  90% of sequence in fragments shorter than   10173 bp
  95% of sequence in fragments shorter than   12955 bp

# KasI.PpuMI (GGCGCC+RGGWCCY) double digest, 1315 thousand fragments (230381+1085138 sites), 2265 bp average fragment length
   5% of sequence in fragments shorter than     727 bp
  10% of sequence in fragments shorter than    1190 bp
  15% of sequence in fragments shorter than    1616 bp
  20% of sequence in fragments shorter than    2024 bp
  25% of sequence in fragments shorter than    2440 bp
  30% of sequence in fragments shorter than    2865 bp
  35% of sequence in fragments shorter than    3298 bp
  40% of sequence in fragments shorter than    3718 bp
  45% of sequence in fragments shorter than    4192 bp
  50% of sequence in fragments shorter than    4720 bp
  55% of sequence in fragments shorter than    5295 bp
  60% of sequence in fragments shorter than    5935 bp
  65% of sequence in fragments shorter than    6655 bp
  70% of sequence in fragments shorter than    7482 bp
  75% of sequence in fragments shorter than    8455 bp
  80% of sequence in fragments shorter than    9663 bp
  85% of sequence in fragments shorter than   11292 bp
  90% of sequence in fragments shorter than   13675 bp
  95% of sequence in fragments shorter than   18717 bp

# HpaI.StuI (GTTAAC+AGGCCT) double digest, 1314 thousand fragments (389418+925729 sites), 2266 bp average fragment length
   5% of sequence in fragments shorter than     697 bp
  10% of sequence in fragments shorter than    1168 bp
  15% of sequence in fragments shorter than    1534 bp
  20% of sequence in fragments shorter than    1921 bp
  25% of sequence in fragments shorter than    2283 bp
  30% of sequence in fragments shorter than    2658 bp
  35% of sequence in fragments shorter than    3036 bp
  40% of sequence in fragments shorter than    3426 bp
  45% of sequence in fragments shorter than    3830 bp
  50% of sequence in fragments shorter than    4258 bp
  55% of sequence in fragments shorter than    4712 bp
  60% of sequence in fragments shorter than    5214 bp
  65% of sequence in fragments shorter than    5761 bp
  70% of sequence in fragments shorter than    6376 bp
  75% of sequence in fragments shorter than    7093 bp
  80% of sequence in fragments shorter than    7936 bp
  85% of sequence in fragments shorter than    9021 bp
  90% of sequence in fragments shorter than   10519 bp
  95% of sequence in fragments shorter than   13027 bp

# KasI.PvuII (GGCGCC+CAGCTG) double digest, 1314 thousand fragments (230381+1084260 sites), 2267 bp average fragment length
   5% of sequence in fragments shorter than     752 bp
  10% of sequence in fragments shorter than    1182 bp
  15% of sequence in fragments shorter than    1572 bp
  20% of sequence in fragments shorter than    1951 bp
  25% of sequence in fragments shorter than    2331 bp
  30% of sequence in fragments shorter than    2717 bp
  35% of sequence in fragments shorter than    3109 bp
  40% of sequence in fragments shorter than    3533 bp
  45% of sequence in fragments shorter than    3984 bp
  50% of sequence in fragments shorter than    4474 bp
  55% of sequence in fragments shorter than    5012 bp
  60% of sequence in fragments shorter than    5605 bp
  65% of sequence in fragments shorter than    6269 bp
  70% of sequence in fragments shorter than    7014 bp
  75% of sequence in fragments shorter than    7904 bp
  80% of sequence in fragments shorter than    8996 bp
  85% of sequence in fragments shorter than   10474 bp
  90% of sequence in fragments shorter than   12620 bp
  95% of sequence in fragments shorter than   16973 bp

# BspHI.BstEII (TCATGA+GGTNACC) double digest, 1313 thousand fragments (978290+335865 sites), 2268 bp average fragment length
   5% of sequence in fragments shorter than     801 bp
  10% of sequence in fragments shorter than    1214 bp
  15% of sequence in fragments shorter than    1573 bp
  20% of sequence in fragments shorter than    1904 bp
  25% of sequence in fragments shorter than    2206 bp
  30% of sequence in fragments shorter than    2517 bp
  35% of sequence in fragments shorter than    2809 bp
  40% of sequence in fragments shorter than    3134 bp
  45% of sequence in fragments shorter than    3474 bp
  50% of sequence in fragments shorter than    3844 bp
  55% of sequence in fragments shorter than    4235 bp
  60% of sequence in fragments shorter than    4664 bp
  65% of sequence in fragments shorter than    5141 bp
  70% of sequence in fragments shorter than    5688 bp
  75% of sequence in fragments shorter than    6323 bp
  80% of sequence in fragments shorter than    7084 bp
  85% of sequence in fragments shorter than    8071 bp
  90% of sequence in fragments shorter than    9508 bp
  95% of sequence in fragments shorter than   12327 bp

# PpuMI.SwaI (RGGWCCY+ATTTAAAT) double digest, 1311 thousand fragments (1085138+226602 sites), 2272 bp average fragment length
   5% of sequence in fragments shorter than     761 bp
  10% of sequence in fragments shorter than    1205 bp
  15% of sequence in fragments shorter than    1600 bp
  20% of sequence in fragments shorter than    1972 bp
  25% of sequence in fragments shorter than    2343 bp
  30% of sequence in fragments shorter than    2719 bp
  35% of sequence in fragments shorter than    3106 bp
  40% of sequence in fragments shorter than    3498 bp
  45% of sequence in fragments shorter than    3875 bp
  50% of sequence in fragments shorter than    4321 bp
  55% of sequence in fragments shorter than    4810 bp
  60% of sequence in fragments shorter than    5341 bp
  65% of sequence in fragments shorter than    5932 bp
  70% of sequence in fragments shorter than    6608 bp
  75% of sequence in fragments shorter than    7413 bp
  80% of sequence in fragments shorter than    8389 bp
  85% of sequence in fragments shorter than    9668 bp
  90% of sequence in fragments shorter than   11573 bp
  95% of sequence in fragments shorter than   15430 bp

# ApaI.XbaI (GGGCCC+TCTAGA) double digest, 1310 thousand fragments (460339+850998 sites), 2273 bp average fragment length
   5% of sequence in fragments shorter than     786 bp
  10% of sequence in fragments shorter than    1198 bp
  15% of sequence in fragments shorter than    1552 bp
  20% of sequence in fragments shorter than    1902 bp
  25% of sequence in fragments shorter than    2245 bp
  30% of sequence in fragments shorter than    2597 bp
  35% of sequence in fragments shorter than    2956 bp
  40% of sequence in fragments shorter than    3327 bp
  45% of sequence in fragments shorter than    3709 bp
  50% of sequence in fragments shorter than    4118 bp
  55% of sequence in fragments shorter than    4556 bp
  60% of sequence in fragments shorter than    5033 bp
  65% of sequence in fragments shorter than    5561 bp
  70% of sequence in fragments shorter than    6160 bp
  75% of sequence in fragments shorter than    6864 bp
  80% of sequence in fragments shorter than    7705 bp
  85% of sequence in fragments shorter than    8775 bp
  90% of sequence in fragments shorter than   10307 bp
  95% of sequence in fragments shorter than   13001 bp

# PvuII.SwaI (CAGCTG+ATTTAAAT) double digest, 1310 thousand fragments (1084260+226602 sites), 2273 bp average fragment length
   5% of sequence in fragments shorter than     784 bp
  10% of sequence in fragments shorter than    1200 bp
  15% of sequence in fragments shorter than    1568 bp
  20% of sequence in fragments shorter than    1921 bp
  25% of sequence in fragments shorter than    2272 bp
  30% of sequence in fragments shorter than    2624 bp
  35% of sequence in fragments shorter than    2969 bp
  40% of sequence in fragments shorter than    3344 bp
  45% of sequence in fragments shorter than    3736 bp
  50% of sequence in fragments shorter than    4160 bp
  55% of sequence in fragments shorter than    4615 bp
  60% of sequence in fragments shorter than    5117 bp
  65% of sequence in fragments shorter than    5672 bp
  70% of sequence in fragments shorter than    6291 bp
  75% of sequence in fragments shorter than    7017 bp
  80% of sequence in fragments shorter than    7909 bp
  85% of sequence in fragments shorter than    9064 bp
  90% of sequence in fragments shorter than   10747 bp
  95% of sequence in fragments shorter than   14087 bp

# BclI.HgaI (TGATCA+GACGC) double digest, 1310 thousand fragments (738785+571889 sites), 2274 bp average fragment length
   5% of sequence in fragments shorter than     795 bp
  10% of sequence in fragments shorter than    1218 bp
  15% of sequence in fragments shorter than    1597 bp
  20% of sequence in fragments shorter than    1945 bp
  25% of sequence in fragments shorter than    2297 bp
  30% of sequence in fragments shorter than    2641 bp
  35% of sequence in fragments shorter than    2986 bp
  40% of sequence in fragments shorter than    3349 bp
  45% of sequence in fragments shorter than    3727 bp
  50% of sequence in fragments shorter than    4132 bp
  55% of sequence in fragments shorter than    4565 bp
  60% of sequence in fragments shorter than    5038 bp
  65% of sequence in fragments shorter than    5560 bp
  70% of sequence in fragments shorter than    6162 bp
  75% of sequence in fragments shorter than    6857 bp
  80% of sequence in fragments shorter than    7704 bp
  85% of sequence in fragments shorter than    8792 bp
  90% of sequence in fragments shorter than   10382 bp
  95% of sequence in fragments shorter than   13338 bp

# NcoI.SphI (CCATGG+GCATGC) double digest, 1310 thousand fragments (759594+550952 sites), 2274 bp average fragment length
   5% of sequence in fragments shorter than     791 bp
  10% of sequence in fragments shorter than    1209 bp
  15% of sequence in fragments shorter than    1564 bp
  20% of sequence in fragments shorter than    1907 bp
  25% of sequence in fragments shorter than    2242 bp
  30% of sequence in fragments shorter than    2580 bp
  35% of sequence in fragments shorter than    2929 bp
  40% of sequence in fragments shorter than    3291 bp
  45% of sequence in fragments shorter than    3670 bp
  50% of sequence in fragments shorter than    4069 bp
  55% of sequence in fragments shorter than    4500 bp
  60% of sequence in fragments shorter than    4951 bp
  65% of sequence in fragments shorter than    5463 bp
  70% of sequence in fragments shorter than    6040 bp
  75% of sequence in fragments shorter than    6724 bp
  80% of sequence in fragments shorter than    7572 bp
  85% of sequence in fragments shorter than    8656 bp
  90% of sequence in fragments shorter than   10246 bp
  95% of sequence in fragments shorter than   13325 bp

# AvaIII.SapI (ATGCAT+GCTCTTC) double digest, 1310 thousand fragments (933357+377161 sites), 2274 bp average fragment length
   5% of sequence in fragments shorter than     797 bp
  10% of sequence in fragments shorter than    1216 bp
  15% of sequence in fragments shorter than    1576 bp
  20% of sequence in fragments shorter than    1917 bp
  25% of sequence in fragments shorter than    2238 bp
  30% of sequence in fragments shorter than    2570 bp
  35% of sequence in fragments shorter than    2903 bp
  40% of sequence in fragments shorter than    3255 bp
  45% of sequence in fragments shorter than    3616 bp
  50% of sequence in fragments shorter than    4001 bp
  55% of sequence in fragments shorter than    4414 bp
  60% of sequence in fragments shorter than    4861 bp
  65% of sequence in fragments shorter than    5356 bp
  70% of sequence in fragments shorter than    5921 bp
  75% of sequence in fragments shorter than    6588 bp
  80% of sequence in fragments shorter than    7411 bp
  85% of sequence in fragments shorter than    8477 bp
  90% of sequence in fragments shorter than   10051 bp
  95% of sequence in fragments shorter than   13154 bp

# AvaIII.SmaI (ATGCAT+CCCGGG) double digest, 1309 thousand fragments (933357+376940 sites), 2275 bp average fragment length
   5% of sequence in fragments shorter than     798 bp
  10% of sequence in fragments shorter than    1217 bp
  15% of sequence in fragments shorter than    1581 bp
  20% of sequence in fragments shorter than    1923 bp
  25% of sequence in fragments shorter than    2247 bp
  30% of sequence in fragments shorter than    2583 bp
  35% of sequence in fragments shorter than    2919 bp
  40% of sequence in fragments shorter than    3275 bp
  45% of sequence in fragments shorter than    3638 bp
  50% of sequence in fragments shorter than    4034 bp
  55% of sequence in fragments shorter than    4461 bp
  60% of sequence in fragments shorter than    4925 bp
  65% of sequence in fragments shorter than    5439 bp
  70% of sequence in fragments shorter than    6031 bp
  75% of sequence in fragments shorter than    6725 bp
  80% of sequence in fragments shorter than    7568 bp
  85% of sequence in fragments shorter than    8646 bp
  90% of sequence in fragments shorter than   10221 bp
  95% of sequence in fragments shorter than   13225 bp

# BclI.EciI (TGATCA+GGCGGA) double digest, 1309 thousand fragments (738785+571273 sites), 2275 bp average fragment length
   5% of sequence in fragments shorter than     784 bp
  10% of sequence in fragments shorter than    1197 bp
  15% of sequence in fragments shorter than    1566 bp
  20% of sequence in fragments shorter than    1910 bp
  25% of sequence in fragments shorter than    2253 bp
  30% of sequence in fragments shorter than    2592 bp
  35% of sequence in fragments shorter than    2935 bp
  40% of sequence in fragments shorter than    3296 bp
  45% of sequence in fragments shorter than    3678 bp
  50% of sequence in fragments shorter than    4085 bp
  55% of sequence in fragments shorter than    4520 bp
  60% of sequence in fragments shorter than    4997 bp
  65% of sequence in fragments shorter than    5523 bp
  70% of sequence in fragments shorter than    6125 bp
  75% of sequence in fragments shorter than    6840 bp
  80% of sequence in fragments shorter than    7721 bp
  85% of sequence in fragments shorter than    8865 bp
  90% of sequence in fragments shorter than   10568 bp
  95% of sequence in fragments shorter than   13869 bp

# AvrII.BsaAI (CCTAGG+YACGTR) double digest, 1308 thousand fragments (594956+713801 sites), 2277 bp average fragment length
   5% of sequence in fragments shorter than     779 bp
  10% of sequence in fragments shorter than    1195 bp
  15% of sequence in fragments shorter than    1561 bp
  20% of sequence in fragments shorter than    1905 bp
  25% of sequence in fragments shorter than    2221 bp
  30% of sequence in fragments shorter than    2559 bp
  35% of sequence in fragments shorter than    2896 bp
  40% of sequence in fragments shorter than    3236 bp
  45% of sequence in fragments shorter than    3604 bp
  50% of sequence in fragments shorter than    3995 bp
  55% of sequence in fragments shorter than    4416 bp
  60% of sequence in fragments shorter than    4875 bp
  65% of sequence in fragments shorter than    5380 bp
  70% of sequence in fragments shorter than    5946 bp
  75% of sequence in fragments shorter than    6607 bp
  80% of sequence in fragments shorter than    7413 bp
  85% of sequence in fragments shorter than    8449 bp
  90% of sequence in fragments shorter than    9916 bp
  95% of sequence in fragments shorter than   12610 bp

# BceAI.SacI (ACGGC+GAGCTC) double digest, 1308 thousand fragments (705178+603323 sites), 2278 bp average fragment length
   5% of sequence in fragments shorter than     750 bp
  10% of sequence in fragments shorter than    1195 bp
  15% of sequence in fragments shorter than    1590 bp
  20% of sequence in fragments shorter than    1980 bp
  25% of sequence in fragments shorter than    2372 bp
  30% of sequence in fragments shorter than    2775 bp
  35% of sequence in fragments shorter than    3195 bp
  40% of sequence in fragments shorter than    3634 bp
  45% of sequence in fragments shorter than    4105 bp
  50% of sequence in fragments shorter than    4598 bp
  55% of sequence in fragments shorter than    5143 bp
  60% of sequence in fragments shorter than    5737 bp
  65% of sequence in fragments shorter than    6405 bp
  70% of sequence in fragments shorter than    7183 bp
  75% of sequence in fragments shorter than    8096 bp
  80% of sequence in fragments shorter than    9188 bp
  85% of sequence in fragments shorter than   10626 bp
  90% of sequence in fragments shorter than   12695 bp
  95% of sequence in fragments shorter than   16463 bp

# ApaI.EcoRI (GGGCCC+GAATTC) double digest, 1307 thousand fragments (460339+847401 sites), 2279 bp average fragment length
   5% of sequence in fragments shorter than     742 bp
  10% of sequence in fragments shorter than    1197 bp
  15% of sequence in fragments shorter than    1588 bp
  20% of sequence in fragments shorter than    1964 bp
  25% of sequence in fragments shorter than    2331 bp
  30% of sequence in fragments shorter than    2695 bp
  35% of sequence in fragments shorter than    3059 bp
  40% of sequence in fragments shorter than    3429 bp
  45% of sequence in fragments shorter than    3824 bp
  50% of sequence in fragments shorter than    4247 bp
  55% of sequence in fragments shorter than    4694 bp
  60% of sequence in fragments shorter than    5190 bp
  65% of sequence in fragments shorter than    5738 bp
  70% of sequence in fragments shorter than    6353 bp
  75% of sequence in fragments shorter than    7068 bp
  80% of sequence in fragments shorter than    7936 bp
  85% of sequence in fragments shorter than    9028 bp
  90% of sequence in fragments shorter than   10581 bp
  95% of sequence in fragments shorter than   13361 bp

# EcoRV.HindIII (GATATC+AAGCTT) double digest, 1306 thousand fragments (446473+860365 sites), 2281 bp average fragment length
   5% of sequence in fragments shorter than     763 bp
  10% of sequence in fragments shorter than    1194 bp
  15% of sequence in fragments shorter than    1543 bp
  20% of sequence in fragments shorter than    1886 bp
  25% of sequence in fragments shorter than    2218 bp
  30% of sequence in fragments shorter than    2555 bp
  35% of sequence in fragments shorter than    2893 bp
  40% of sequence in fragments shorter than    3243 bp
  45% of sequence in fragments shorter than    3613 bp
  50% of sequence in fragments shorter than    4003 bp
  55% of sequence in fragments shorter than    4425 bp
  60% of sequence in fragments shorter than    4885 bp
  65% of sequence in fragments shorter than    5399 bp
  70% of sequence in fragments shorter than    5994 bp
  75% of sequence in fragments shorter than    6697 bp
  80% of sequence in fragments shorter than    7560 bp
  85% of sequence in fragments shorter than    8688 bp
  90% of sequence in fragments shorter than   10342 bp
  95% of sequence in fragments shorter than   13546 bp

# FnuDII.HgaI (CGCG+GACGC) double digest, 1305 thousand fragments (733940+571889 sites), 2282 bp average fragment length
   5% of sequence in fragments shorter than     910 bp
  10% of sequence in fragments shorter than    1612 bp
  15% of sequence in fragments shorter than    2261 bp
  20% of sequence in fragments shorter than    2898 bp
  25% of sequence in fragments shorter than    3557 bp
  30% of sequence in fragments shorter than    4255 bp
  35% of sequence in fragments shorter than    4995 bp
  40% of sequence in fragments shorter than    5784 bp
  45% of sequence in fragments shorter than    6626 bp
  50% of sequence in fragments shorter than    7541 bp
  55% of sequence in fragments shorter than    8564 bp
  60% of sequence in fragments shorter than    9693 bp
  65% of sequence in fragments shorter than   10978 bp
  70% of sequence in fragments shorter than   12467 bp
  75% of sequence in fragments shorter than   14245 bp
  80% of sequence in fragments shorter than   16450 bp
  85% of sequence in fragments shorter than   19329 bp
  90% of sequence in fragments shorter than   23435 bp
  95% of sequence in fragments shorter than   31295 bp

# EciI.FnuDII (GGCGGA+CGCG) double digest, 1304 thousand fragments (571273+733940 sites), 2283 bp average fragment length
   5% of sequence in fragments shorter than     875 bp
  10% of sequence in fragments shorter than    1575 bp
  15% of sequence in fragments shorter than    2244 bp
  20% of sequence in fragments shorter than    2920 bp
  25% of sequence in fragments shorter than    3629 bp
  30% of sequence in fragments shorter than    4403 bp
  35% of sequence in fragments shorter than    5240 bp
  40% of sequence in fragments shorter than    6153 bp
  45% of sequence in fragments shorter than    7139 bp
  50% of sequence in fragments shorter than    8238 bp
  55% of sequence in fragments shorter than    9474 bp
  60% of sequence in fragments shorter than   10872 bp
  65% of sequence in fragments shorter than   12467 bp
  70% of sequence in fragments shorter than   14344 bp
  75% of sequence in fragments shorter than   16641 bp
  80% of sequence in fragments shorter than   19477 bp
  85% of sequence in fragments shorter than   23221 bp
  90% of sequence in fragments shorter than   28778 bp
  95% of sequence in fragments shorter than   39827 bp

# AvaI.SexAI (CYCGRG+ACCWGGT) double digest, 1303 thousand fragments (1005714+297900 sites), 2286 bp average fragment length
   5% of sequence in fragments shorter than     742 bp
  10% of sequence in fragments shorter than    1203 bp
  15% of sequence in fragments shorter than    1631 bp
  20% of sequence in fragments shorter than    2051 bp
  25% of sequence in fragments shorter than    2484 bp
  30% of sequence in fragments shorter than    2938 bp
  35% of sequence in fragments shorter than    3418 bp
  40% of sequence in fragments shorter than    3934 bp
  45% of sequence in fragments shorter than    4481 bp
  50% of sequence in fragments shorter than    5087 bp
  55% of sequence in fragments shorter than    5751 bp
  60% of sequence in fragments shorter than    6498 bp
  65% of sequence in fragments shorter than    7339 bp
  70% of sequence in fragments shorter than    8324 bp
  75% of sequence in fragments shorter than    9491 bp
  80% of sequence in fragments shorter than   10925 bp
  85% of sequence in fragments shorter than   12838 bp
  90% of sequence in fragments shorter than   15741 bp
  95% of sequence in fragments shorter than   21840 bp

# NdeI.SpeI (CATATG+ACTAGT) double digest, 1303 thousand fragments (903360+400286 sites), 2286 bp average fragment length
   5% of sequence in fragments shorter than     747 bp
  10% of sequence in fragments shorter than    1167 bp
  15% of sequence in fragments shorter than    1549 bp
  20% of sequence in fragments shorter than    1910 bp
  25% of sequence in fragments shorter than    2261 bp
  30% of sequence in fragments shorter than    2618 bp
  35% of sequence in fragments shorter than    2985 bp
  40% of sequence in fragments shorter than    3371 bp
  45% of sequence in fragments shorter than    3783 bp
  50% of sequence in fragments shorter than    4217 bp
  55% of sequence in fragments shorter than    4689 bp
  60% of sequence in fragments shorter than    5198 bp
  65% of sequence in fragments shorter than    5774 bp
  70% of sequence in fragments shorter than    6452 bp
  75% of sequence in fragments shorter than    7258 bp
  80% of sequence in fragments shorter than    8273 bp
  85% of sequence in fragments shorter than    9693 bp
  90% of sequence in fragments shorter than   11922 bp
  95% of sequence in fragments shorter than   16982 bp

# NcoI.ScaI (CCATGG+AGTACT) double digest, 1302 thousand fragments (759594+543793 sites), 2287 bp average fragment length
   5% of sequence in fragments shorter than     807 bp
  10% of sequence in fragments shorter than    1219 bp
  15% of sequence in fragments shorter than    1567 bp
  20% of sequence in fragments shorter than    1896 bp
  25% of sequence in fragments shorter than    2222 bp
  30% of sequence in fragments shorter than    2546 bp
  35% of sequence in fragments shorter than    2872 bp
  40% of sequence in fragments shorter than    3213 bp
  45% of sequence in fragments shorter than    3567 bp
  50% of sequence in fragments shorter than    3945 bp
  55% of sequence in fragments shorter than    4351 bp
  60% of sequence in fragments shorter than    4774 bp
  65% of sequence in fragments shorter than    5261 bp
  70% of sequence in fragments shorter than    5798 bp
  75% of sequence in fragments shorter than    6421 bp
  80% of sequence in fragments shorter than    7195 bp
  85% of sequence in fragments shorter than    8199 bp
  90% of sequence in fragments shorter than    9649 bp
  95% of sequence in fragments shorter than   12440 bp

# BclI.MfeI (TGATCA+CAATTG) double digest, 1302 thousand fragments (738785+564303 sites), 2287 bp average fragment length
   5% of sequence in fragments shorter than     791 bp
  10% of sequence in fragments shorter than    1193 bp
  15% of sequence in fragments shorter than    1555 bp
  20% of sequence in fragments shorter than    1891 bp
  25% of sequence in fragments shorter than    2211 bp
  30% of sequence in fragments shorter than    2537 bp
  35% of sequence in fragments shorter than    2868 bp
  40% of sequence in fragments shorter than    3210 bp
  45% of sequence in fragments shorter than    3574 bp
  50% of sequence in fragments shorter than    3961 bp
  55% of sequence in fragments shorter than    4368 bp
  60% of sequence in fragments shorter than    4819 bp
  65% of sequence in fragments shorter than    5320 bp
  70% of sequence in fragments shorter than    5899 bp
  75% of sequence in fragments shorter than    6581 bp
  80% of sequence in fragments shorter than    7410 bp
  85% of sequence in fragments shorter than    8502 bp
  90% of sequence in fragments shorter than   10149 bp
  95% of sequence in fragments shorter than   13399 bp

# SapI.StuI (GCTCTTC+AGGCCT) double digest, 1302 thousand fragments (377161+925729 sites), 2288 bp average fragment length
   5% of sequence in fragments shorter than     691 bp
  10% of sequence in fragments shorter than    1155 bp
  15% of sequence in fragments shorter than    1549 bp
  20% of sequence in fragments shorter than    1965 bp
  25% of sequence in fragments shorter than    2370 bp
  30% of sequence in fragments shorter than    2779 bp
  35% of sequence in fragments shorter than    3201 bp
  40% of sequence in fragments shorter than    3636 bp
  45% of sequence in fragments shorter than    4089 bp
  50% of sequence in fragments shorter than    4575 bp
  55% of sequence in fragments shorter than    5100 bp
  60% of sequence in fragments shorter than    5667 bp
  65% of sequence in fragments shorter than    6296 bp
  70% of sequence in fragments shorter than    7017 bp
  75% of sequence in fragments shorter than    7849 bp
  80% of sequence in fragments shorter than    8857 bp
  85% of sequence in fragments shorter than   10127 bp
  90% of sequence in fragments shorter than   11899 bp
  95% of sequence in fragments shorter than   14963 bp

# SmaI.StuI (CCCGGG+AGGCCT) double digest, 1302 thousand fragments (376940+925729 sites), 2288 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1143 bp
  15% of sequence in fragments shorter than    1552 bp
  20% of sequence in fragments shorter than    1977 bp
  25% of sequence in fragments shorter than    2385 bp
  30% of sequence in fragments shorter than    2826 bp
  35% of sequence in fragments shorter than    3281 bp
  40% of sequence in fragments shorter than    3752 bp
  45% of sequence in fragments shorter than    4253 bp
  50% of sequence in fragments shorter than    4795 bp
  55% of sequence in fragments shorter than    5382 bp
  60% of sequence in fragments shorter than    6018 bp
  65% of sequence in fragments shorter than    6741 bp
  70% of sequence in fragments shorter than    7560 bp
  75% of sequence in fragments shorter than    8507 bp
  80% of sequence in fragments shorter than    9670 bp
  85% of sequence in fragments shorter than   11143 bp
  90% of sequence in fragments shorter than   13228 bp
  95% of sequence in fragments shorter than   16774 bp

# AatII.BscGI (GACGTC+CCCGT) double digest, 1301 thousand fragments (71089+1231070 sites), 2289 bp average fragment length
   5% of sequence in fragments shorter than     665 bp
  10% of sequence in fragments shorter than    1152 bp
  15% of sequence in fragments shorter than    1625 bp
  20% of sequence in fragments shorter than    2093 bp
  25% of sequence in fragments shorter than    2568 bp
  30% of sequence in fragments shorter than    3056 bp
  35% of sequence in fragments shorter than    3568 bp
  40% of sequence in fragments shorter than    4109 bp
  45% of sequence in fragments shorter than    4681 bp
  50% of sequence in fragments shorter than    5308 bp
  55% of sequence in fragments shorter than    5996 bp
  60% of sequence in fragments shorter than    6748 bp
  65% of sequence in fragments shorter than    7603 bp
  70% of sequence in fragments shorter than    8593 bp
  75% of sequence in fragments shorter than    9778 bp
  80% of sequence in fragments shorter than   11214 bp
  85% of sequence in fragments shorter than   13057 bp
  90% of sequence in fragments shorter than   15694 bp
  95% of sequence in fragments shorter than   20074 bp

# AvrII.BceAI (CCTAGG+ACGGC) double digest, 1299 thousand fragments (594956+705178 sites), 2292 bp average fragment length
   5% of sequence in fragments shorter than     751 bp
  10% of sequence in fragments shorter than    1203 bp
  15% of sequence in fragments shorter than    1602 bp
  20% of sequence in fragments shorter than    1987 bp
  25% of sequence in fragments shorter than    2365 bp
  30% of sequence in fragments shorter than    2749 bp
  35% of sequence in fragments shorter than    3140 bp
  40% of sequence in fragments shorter than    3552 bp
  45% of sequence in fragments shorter than    3963 bp
  50% of sequence in fragments shorter than    4403 bp
  55% of sequence in fragments shorter than    4894 bp
  60% of sequence in fragments shorter than    5441 bp
  65% of sequence in fragments shorter than    6048 bp
  70% of sequence in fragments shorter than    6751 bp
  75% of sequence in fragments shorter than    7577 bp
  80% of sequence in fragments shorter than    8594 bp
  85% of sequence in fragments shorter than    9904 bp
  90% of sequence in fragments shorter than   11815 bp
  95% of sequence in fragments shorter than   15502 bp

# BciVI.SacI (GTATCC+GAGCTC) double digest, 1298 thousand fragments (696093+603323 sites), 2294 bp average fragment length
   5% of sequence in fragments shorter than     808 bp
  10% of sequence in fragments shorter than    1222 bp
  15% of sequence in fragments shorter than    1580 bp
  20% of sequence in fragments shorter than    1896 bp
  25% of sequence in fragments shorter than    2219 bp
  30% of sequence in fragments shorter than    2527 bp
  35% of sequence in fragments shorter than    2854 bp
  40% of sequence in fragments shorter than    3197 bp
  45% of sequence in fragments shorter than    3553 bp
  50% of sequence in fragments shorter than    3932 bp
  55% of sequence in fragments shorter than    4334 bp
  60% of sequence in fragments shorter than    4778 bp
  65% of sequence in fragments shorter than    5267 bp
  70% of sequence in fragments shorter than    5825 bp
  75% of sequence in fragments shorter than    6473 bp
  80% of sequence in fragments shorter than    7252 bp
  85% of sequence in fragments shorter than    8254 bp
  90% of sequence in fragments shorter than    9687 bp
  95% of sequence in fragments shorter than   12296 bp

# AvaIII.BamHI (ATGCAT+GGATCC) double digest, 1298 thousand fragments (933357+365999 sites), 2294 bp average fragment length
   5% of sequence in fragments shorter than     818 bp
  10% of sequence in fragments shorter than    1240 bp
  15% of sequence in fragments shorter than    1592 bp
  20% of sequence in fragments shorter than    1929 bp
  25% of sequence in fragments shorter than    2256 bp
  30% of sequence in fragments shorter than    2584 bp
  35% of sequence in fragments shorter than    2914 bp
  40% of sequence in fragments shorter than    3259 bp
  45% of sequence in fragments shorter than    3617 bp
  50% of sequence in fragments shorter than    4000 bp
  55% of sequence in fragments shorter than    4416 bp
  60% of sequence in fragments shorter than    4869 bp
  65% of sequence in fragments shorter than    5366 bp
  70% of sequence in fragments shorter than    5925 bp
  75% of sequence in fragments shorter than    6575 bp
  80% of sequence in fragments shorter than    7373 bp
  85% of sequence in fragments shorter than    8416 bp
  90% of sequence in fragments shorter than    9879 bp
  95% of sequence in fragments shorter than   12735 bp

# FnuDII.MfeI (CGCG+CAATTG) double digest, 1297 thousand fragments (733940+564303 sites), 2296 bp average fragment length
   5% of sequence in fragments shorter than     906 bp
  10% of sequence in fragments shorter than    1480 bp
  15% of sequence in fragments shorter than    1965 bp
  20% of sequence in fragments shorter than    2408 bp
  25% of sequence in fragments shorter than    2850 bp
  30% of sequence in fragments shorter than    3286 bp
  35% of sequence in fragments shorter than    3735 bp
  40% of sequence in fragments shorter than    4178 bp
  45% of sequence in fragments shorter than    4662 bp
  50% of sequence in fragments shorter than    5169 bp
  55% of sequence in fragments shorter than    5717 bp
  60% of sequence in fragments shorter than    6318 bp
  65% of sequence in fragments shorter than    6981 bp
  70% of sequence in fragments shorter than    7735 bp
  75% of sequence in fragments shorter than    8622 bp
  80% of sequence in fragments shorter than    9687 bp
  85% of sequence in fragments shorter than   11046 bp
  90% of sequence in fragments shorter than   13002 bp
  95% of sequence in fragments shorter than   16632 bp

# BscGI.SacII (CCCGT+CCGCGG) double digest, 1297 thousand fragments (1231070+66901 sites), 2296 bp average fragment length
   5% of sequence in fragments shorter than     663 bp
  10% of sequence in fragments shorter than    1176 bp
  15% of sequence in fragments shorter than    1676 bp
  20% of sequence in fragments shorter than    2170 bp
  25% of sequence in fragments shorter than    2669 bp
  30% of sequence in fragments shorter than    3190 bp
  35% of sequence in fragments shorter than    3728 bp
  40% of sequence in fragments shorter than    4302 bp
  45% of sequence in fragments shorter than    4913 bp
  50% of sequence in fragments shorter than    5581 bp
  55% of sequence in fragments shorter than    6308 bp
  60% of sequence in fragments shorter than    7107 bp
  65% of sequence in fragments shorter than    8017 bp
  70% of sequence in fragments shorter than    9089 bp
  75% of sequence in fragments shorter than   10331 bp
  80% of sequence in fragments shorter than   11856 bp
  85% of sequence in fragments shorter than   13813 bp
  90% of sequence in fragments shorter than   16578 bp
  95% of sequence in fragments shorter than   21338 bp

# EcoRV.XbaI (GATATC+TCTAGA) double digest, 1297 thousand fragments (446473+850998 sites), 2297 bp average fragment length
   5% of sequence in fragments shorter than     802 bp
  10% of sequence in fragments shorter than    1209 bp
  15% of sequence in fragments shorter than    1561 bp
  20% of sequence in fragments shorter than    1901 bp
  25% of sequence in fragments shorter than    2239 bp
  30% of sequence in fragments shorter than    2579 bp
  35% of sequence in fragments shorter than    2935 bp
  40% of sequence in fragments shorter than    3302 bp
  45% of sequence in fragments shorter than    3684 bp
  50% of sequence in fragments shorter than    4091 bp
  55% of sequence in fragments shorter than    4533 bp
  60% of sequence in fragments shorter than    5015 bp
  65% of sequence in fragments shorter than    5555 bp
  70% of sequence in fragments shorter than    6171 bp
  75% of sequence in fragments shorter than    6899 bp
  80% of sequence in fragments shorter than    7782 bp
  85% of sequence in fragments shorter than    8958 bp
  90% of sequence in fragments shorter than   10680 bp
  95% of sequence in fragments shorter than   13860 bp

# EcoRI.EcoRV (GAATTC+GATATC) double digest, 1293 thousand fragments (847401+446473 sites), 2304 bp average fragment length
   5% of sequence in fragments shorter than     759 bp
  10% of sequence in fragments shorter than    1215 bp
  15% of sequence in fragments shorter than    1595 bp
  20% of sequence in fragments shorter than    1952 bp
  25% of sequence in fragments shorter than    2305 bp
  30% of sequence in fragments shorter than    2646 bp
  35% of sequence in fragments shorter than    2995 bp
  40% of sequence in fragments shorter than    3361 bp
  45% of sequence in fragments shorter than    3744 bp
  50% of sequence in fragments shorter than    4150 bp
  55% of sequence in fragments shorter than    4587 bp
  60% of sequence in fragments shorter than    5068 bp
  65% of sequence in fragments shorter than    5602 bp
  70% of sequence in fragments shorter than    6205 bp
  75% of sequence in fragments shorter than    6917 bp
  80% of sequence in fragments shorter than    7792 bp
  85% of sequence in fragments shorter than    8913 bp
  90% of sequence in fragments shorter than   10518 bp
  95% of sequence in fragments shorter than   13560 bp

# AvaI.KpnI (CYCGRG+GGTACC) double digest, 1293 thousand fragments (1005714+287974 sites), 2304 bp average fragment length
   5% of sequence in fragments shorter than     762 bp
  10% of sequence in fragments shorter than    1222 bp
  15% of sequence in fragments shorter than    1624 bp
  20% of sequence in fragments shorter than    2040 bp
  25% of sequence in fragments shorter than    2473 bp
  30% of sequence in fragments shorter than    2915 bp
  35% of sequence in fragments shorter than    3383 bp
  40% of sequence in fragments shorter than    3877 bp
  45% of sequence in fragments shorter than    4409 bp
  50% of sequence in fragments shorter than    4990 bp
  55% of sequence in fragments shorter than    5629 bp
  60% of sequence in fragments shorter than    6349 bp
  65% of sequence in fragments shorter than    7165 bp
  70% of sequence in fragments shorter than    8121 bp
  75% of sequence in fragments shorter than    9252 bp
  80% of sequence in fragments shorter than   10635 bp
  85% of sequence in fragments shorter than   12470 bp
  90% of sequence in fragments shorter than   15278 bp
  95% of sequence in fragments shorter than   20951 bp

# AvaI.NheI (CYCGRG+GCTAGC) double digest, 1292 thousand fragments (1005714+287384 sites), 2305 bp average fragment length
   5% of sequence in fragments shorter than     776 bp
  10% of sequence in fragments shorter than    1246 bp
  15% of sequence in fragments shorter than    1660 bp
  20% of sequence in fragments shorter than    2035 bp
  25% of sequence in fragments shorter than    2456 bp
  30% of sequence in fragments shorter than    2893 bp
  35% of sequence in fragments shorter than    3354 bp
  40% of sequence in fragments shorter than    3847 bp
  45% of sequence in fragments shorter than    4372 bp
  50% of sequence in fragments shorter than    4947 bp
  55% of sequence in fragments shorter than    5568 bp
  60% of sequence in fragments shorter than    6269 bp
  65% of sequence in fragments shorter than    7063 bp
  70% of sequence in fragments shorter than    7972 bp
  75% of sequence in fragments shorter than    9076 bp
  80% of sequence in fragments shorter than   10410 bp
  85% of sequence in fragments shorter than   12214 bp
  90% of sequence in fragments shorter than   14841 bp
  95% of sequence in fragments shorter than   20002 bp

# HpaI.NdeI (GTTAAC+CATATG) double digest, 1292 thousand fragments (389418+903360 sites), 2305 bp average fragment length
   5% of sequence in fragments shorter than     785 bp
  10% of sequence in fragments shorter than    1178 bp
  15% of sequence in fragments shorter than    1552 bp
  20% of sequence in fragments shorter than    1910 bp
  25% of sequence in fragments shorter than    2248 bp
  30% of sequence in fragments shorter than    2602 bp
  35% of sequence in fragments shorter than    2962 bp
  40% of sequence in fragments shorter than    3336 bp
  45% of sequence in fragments shorter than    3734 bp
  50% of sequence in fragments shorter than    4159 bp
  55% of sequence in fragments shorter than    4623 bp
  60% of sequence in fragments shorter than    5127 bp
  65% of sequence in fragments shorter than    5691 bp
  70% of sequence in fragments shorter than    6349 bp
  75% of sequence in fragments shorter than    7140 bp
  80% of sequence in fragments shorter than    8145 bp
  85% of sequence in fragments shorter than    9512 bp
  90% of sequence in fragments shorter than   11663 bp
  95% of sequence in fragments shorter than   16603 bp

# BamHI.StuI (GGATCC+AGGCCT) double digest, 1291 thousand fragments (365999+925729 sites), 2307 bp average fragment length
   5% of sequence in fragments shorter than     698 bp
  10% of sequence in fragments shorter than    1166 bp
  15% of sequence in fragments shorter than    1566 bp
  20% of sequence in fragments shorter than    1979 bp
  25% of sequence in fragments shorter than    2373 bp
  30% of sequence in fragments shorter than    2787 bp
  35% of sequence in fragments shorter than    3197 bp
  40% of sequence in fragments shorter than    3634 bp
  45% of sequence in fragments shorter than    4100 bp
  50% of sequence in fragments shorter than    4594 bp
  55% of sequence in fragments shorter than    5129 bp
  60% of sequence in fragments shorter than    5710 bp
  65% of sequence in fragments shorter than    6365 bp
  70% of sequence in fragments shorter than    7103 bp
  75% of sequence in fragments shorter than    7957 bp
  80% of sequence in fragments shorter than    8988 bp
  85% of sequence in fragments shorter than   10302 bp
  90% of sequence in fragments shorter than   12135 bp
  95% of sequence in fragments shorter than   15269 bp

# AvrII.BciVI (CCTAGG+GTATCC) double digest, 1290 thousand fragments (594956+696093 sites), 2308 bp average fragment length
   5% of sequence in fragments shorter than     807 bp
  10% of sequence in fragments shorter than    1238 bp
  15% of sequence in fragments shorter than    1608 bp
  20% of sequence in fragments shorter than    1942 bp
  25% of sequence in fragments shorter than    2274 bp
  30% of sequence in fragments shorter than    2609 bp
  35% of sequence in fragments shorter than    2951 bp
  40% of sequence in fragments shorter than    3299 bp
  45% of sequence in fragments shorter than    3664 bp
  50% of sequence in fragments shorter than    4048 bp
  55% of sequence in fragments shorter than    4454 bp
  60% of sequence in fragments shorter than    4896 bp
  65% of sequence in fragments shorter than    5397 bp
  70% of sequence in fragments shorter than    5953 bp
  75% of sequence in fragments shorter than    6602 bp
  80% of sequence in fragments shorter than    7398 bp
  85% of sequence in fragments shorter than    8420 bp
  90% of sequence in fragments shorter than    9889 bp
  95% of sequence in fragments shorter than   12613 bp

# BscGI.PspXI (CCCGT+VCTCGAGB) double digest, 1289 thousand fragments (1231070+59193 sites), 2310 bp average fragment length
   5% of sequence in fragments shorter than     670 bp
  10% of sequence in fragments shorter than    1161 bp
  15% of sequence in fragments shorter than    1639 bp
  20% of sequence in fragments shorter than    2111 bp
  25% of sequence in fragments shorter than    2590 bp
  30% of sequence in fragments shorter than    3087 bp
  35% of sequence in fragments shorter than    3606 bp
  40% of sequence in fragments shorter than    4154 bp
  45% of sequence in fragments shorter than    4740 bp
  50% of sequence in fragments shorter than    5383 bp
  55% of sequence in fragments shorter than    6085 bp
  60% of sequence in fragments shorter than    6848 bp
  65% of sequence in fragments shorter than    7721 bp
  70% of sequence in fragments shorter than    8734 bp
  75% of sequence in fragments shorter than    9932 bp
  80% of sequence in fragments shorter than   11393 bp
  85% of sequence in fragments shorter than   13259 bp
  90% of sequence in fragments shorter than   15937 bp
  95% of sequence in fragments shorter than   20446 bp

# BclI.SphI (TGATCA+GCATGC) double digest, 1289 thousand fragments (738785+550952 sites), 2311 bp average fragment length
   5% of sequence in fragments shorter than     821 bp
  10% of sequence in fragments shorter than    1231 bp
  15% of sequence in fragments shorter than    1587 bp
  20% of sequence in fragments shorter than    1916 bp
  25% of sequence in fragments shorter than    2244 bp
  30% of sequence in fragments shorter than    2565 bp
  35% of sequence in fragments shorter than    2886 bp
  40% of sequence in fragments shorter than    3222 bp
  45% of sequence in fragments shorter than    3575 bp
  50% of sequence in fragments shorter than    3950 bp
  55% of sequence in fragments shorter than    4340 bp
  60% of sequence in fragments shorter than    4777 bp
  65% of sequence in fragments shorter than    5257 bp
  70% of sequence in fragments shorter than    5803 bp
  75% of sequence in fragments shorter than    6441 bp
  80% of sequence in fragments shorter than    7206 bp
  85% of sequence in fragments shorter than    8202 bp
  90% of sequence in fragments shorter than    9658 bp
  95% of sequence in fragments shorter than   12539 bp

# BsaAI.HgaI (YACGTR+GACGC) double digest, 1285 thousand fragments (713801+571889 sites), 2318 bp average fragment length
   5% of sequence in fragments shorter than     794 bp
  10% of sequence in fragments shorter than    1242 bp
  15% of sequence in fragments shorter than    1661 bp
  20% of sequence in fragments shorter than    2047 bp
  25% of sequence in fragments shorter than    2439 bp
  30% of sequence in fragments shorter than    2829 bp
  35% of sequence in fragments shorter than    3241 bp
  40% of sequence in fragments shorter than    3661 bp
  45% of sequence in fragments shorter than    4113 bp
  50% of sequence in fragments shorter than    4591 bp
  55% of sequence in fragments shorter than    5112 bp
  60% of sequence in fragments shorter than    5680 bp
  65% of sequence in fragments shorter than    6312 bp
  70% of sequence in fragments shorter than    7043 bp
  75% of sequence in fragments shorter than    7890 bp
  80% of sequence in fragments shorter than    8922 bp
  85% of sequence in fragments shorter than   10243 bp
  90% of sequence in fragments shorter than   12095 bp
  95% of sequence in fragments shorter than   15568 bp

# BsaAI.EciI (YACGTR+GGCGGA) double digest, 1284 thousand fragments (713801+571273 sites), 2319 bp average fragment length
   5% of sequence in fragments shorter than     791 bp
  10% of sequence in fragments shorter than    1224 bp
  15% of sequence in fragments shorter than    1621 bp
  20% of sequence in fragments shorter than    1995 bp
  25% of sequence in fragments shorter than    2374 bp
  30% of sequence in fragments shorter than    2755 bp
  35% of sequence in fragments shorter than    3150 bp
  40% of sequence in fragments shorter than    3545 bp
  45% of sequence in fragments shorter than    3986 bp
  50% of sequence in fragments shorter than    4449 bp
  55% of sequence in fragments shorter than    4963 bp
  60% of sequence in fragments shorter than    5525 bp
  65% of sequence in fragments shorter than    6165 bp
  70% of sequence in fragments shorter than    6896 bp
  75% of sequence in fragments shorter than    7760 bp
  80% of sequence in fragments shorter than    8807 bp
  85% of sequence in fragments shorter than   10204 bp
  90% of sequence in fragments shorter than   12182 bp
  95% of sequence in fragments shorter than   15812 bp

# FnuDII.SphI (CGCG+GCATGC) double digest, 1284 thousand fragments (733940+550952 sites), 2320 bp average fragment length
   5% of sequence in fragments shorter than     893 bp
  10% of sequence in fragments shorter than    1473 bp
  15% of sequence in fragments shorter than    1987 bp
  20% of sequence in fragments shorter than    2483 bp
  25% of sequence in fragments shorter than    2978 bp
  30% of sequence in fragments shorter than    3490 bp
  35% of sequence in fragments shorter than    4024 bp
  40% of sequence in fragments shorter than    4594 bp
  45% of sequence in fragments shorter than    5204 bp
  50% of sequence in fragments shorter than    5849 bp
  55% of sequence in fragments shorter than    6546 bp
  60% of sequence in fragments shorter than    7319 bp
  65% of sequence in fragments shorter than    8199 bp
  70% of sequence in fragments shorter than    9211 bp
  75% of sequence in fragments shorter than   10404 bp
  80% of sequence in fragments shorter than   11880 bp
  85% of sequence in fragments shorter than   13806 bp
  90% of sequence in fragments shorter than   16593 bp
  95% of sequence in fragments shorter than   21966 bp

# AgeI.BscGI (ACCGGT+CCCGT) double digest, 1283 thousand fragments (53386+1231070 sites), 2320 bp average fragment length
   5% of sequence in fragments shorter than     676 bp
  10% of sequence in fragments shorter than    1168 bp
  15% of sequence in fragments shorter than    1646 bp
  20% of sequence in fragments shorter than    2117 bp
  25% of sequence in fragments shorter than    2595 bp
  30% of sequence in fragments shorter than    3091 bp
  35% of sequence in fragments shorter than    3607 bp
  40% of sequence in fragments shorter than    4155 bp
  45% of sequence in fragments shorter than    4733 bp
  50% of sequence in fragments shorter than    5367 bp
  55% of sequence in fragments shorter than    6063 bp
  60% of sequence in fragments shorter than    6824 bp
  65% of sequence in fragments shorter than    7690 bp
  70% of sequence in fragments shorter than    8694 bp
  75% of sequence in fragments shorter than    9875 bp
  80% of sequence in fragments shorter than   11324 bp
  85% of sequence in fragments shorter than   13163 bp
  90% of sequence in fragments shorter than   15827 bp
  95% of sequence in fragments shorter than   20337 bp

# BclI.ScaI (TGATCA+AGTACT) double digest, 1282 thousand fragments (738785+543793 sites), 2324 bp average fragment length
   5% of sequence in fragments shorter than     816 bp
  10% of sequence in fragments shorter than    1225 bp
  15% of sequence in fragments shorter than    1584 bp
  20% of sequence in fragments shorter than    1917 bp
  25% of sequence in fragments shorter than    2251 bp
  30% of sequence in fragments shorter than    2576 bp
  35% of sequence in fragments shorter than    2904 bp
  40% of sequence in fragments shorter than    3251 bp
  45% of sequence in fragments shorter than    3616 bp
  50% of sequence in fragments shorter than    4003 bp
  55% of sequence in fragments shorter than    4416 bp
  60% of sequence in fragments shorter than    4868 bp
  65% of sequence in fragments shorter than    5373 bp
  70% of sequence in fragments shorter than    5955 bp
  75% of sequence in fragments shorter than    6645 bp
  80% of sequence in fragments shorter than    7493 bp
  85% of sequence in fragments shorter than    8607 bp
  90% of sequence in fragments shorter than   10280 bp
  95% of sequence in fragments shorter than   13843 bp

# NdeI.SapI (CATATG+GCTCTTC) double digest, 1280 thousand fragments (903360+377161 sites), 2327 bp average fragment length
   5% of sequence in fragments shorter than     797 bp
  10% of sequence in fragments shorter than    1199 bp
  15% of sequence in fragments shorter than    1569 bp
  20% of sequence in fragments shorter than    1924 bp
  25% of sequence in fragments shorter than    2253 bp
  30% of sequence in fragments shorter than    2588 bp
  35% of sequence in fragments shorter than    2938 bp
  40% of sequence in fragments shorter than    3293 bp
  45% of sequence in fragments shorter than    3677 bp
  50% of sequence in fragments shorter than    4086 bp
  55% of sequence in fragments shorter than    4521 bp
  60% of sequence in fragments shorter than    5001 bp
  65% of sequence in fragments shorter than    5535 bp
  70% of sequence in fragments shorter than    6145 bp
  75% of sequence in fragments shorter than    6861 bp
  80% of sequence in fragments shorter than    7748 bp
  85% of sequence in fragments shorter than    8918 bp
  90% of sequence in fragments shorter than   10660 bp
  95% of sequence in fragments shorter than   14225 bp

# NdeI.SmaI (CATATG+CCCGGG) double digest, 1279 thousand fragments (903360+376940 sites), 2328 bp average fragment length
   5% of sequence in fragments shorter than     797 bp
  10% of sequence in fragments shorter than    1199 bp
  15% of sequence in fragments shorter than    1585 bp
  20% of sequence in fragments shorter than    1945 bp
  25% of sequence in fragments shorter than    2293 bp
  30% of sequence in fragments shorter than    2646 bp
  35% of sequence in fragments shorter than    3000 bp
  40% of sequence in fragments shorter than    3365 bp
  45% of sequence in fragments shorter than    3747 bp
  50% of sequence in fragments shorter than    4151 bp
  55% of sequence in fragments shorter than    4577 bp
  60% of sequence in fragments shorter than    5040 bp
  65% of sequence in fragments shorter than    5550 bp
  70% of sequence in fragments shorter than    6127 bp
  75% of sequence in fragments shorter than    6802 bp
  80% of sequence in fragments shorter than    7631 bp
  85% of sequence in fragments shorter than    8704 bp
  90% of sequence in fragments shorter than   10275 bp
  95% of sequence in fragments shorter than   13385 bp

# BsaAI.MfeI (YACGTR+CAATTG) double digest, 1277 thousand fragments (713801+564303 sites), 2332 bp average fragment length
   5% of sequence in fragments shorter than     837 bp
  10% of sequence in fragments shorter than    1249 bp
  15% of sequence in fragments shorter than    1599 bp
  20% of sequence in fragments shorter than    1937 bp
  25% of sequence in fragments shorter than    2250 bp
  30% of sequence in fragments shorter than    2588 bp
  35% of sequence in fragments shorter than    2924 bp
  40% of sequence in fragments shorter than    3262 bp
  45% of sequence in fragments shorter than    3622 bp
  50% of sequence in fragments shorter than    4011 bp
  55% of sequence in fragments shorter than    4424 bp
  60% of sequence in fragments shorter than    4869 bp
  65% of sequence in fragments shorter than    5362 bp
  70% of sequence in fragments shorter than    5928 bp
  75% of sequence in fragments shorter than    6574 bp
  80% of sequence in fragments shorter than    7359 bp
  85% of sequence in fragments shorter than    8357 bp
  90% of sequence in fragments shorter than    9772 bp
  95% of sequence in fragments shorter than   12258 bp

# FnuDII.ScaI (CGCG+AGTACT) double digest, 1277 thousand fragments (733940+543793 sites), 2333 bp average fragment length
   5% of sequence in fragments shorter than     920 bp
  10% of sequence in fragments shorter than    1497 bp
  15% of sequence in fragments shorter than    1989 bp
  20% of sequence in fragments shorter than    2458 bp
  25% of sequence in fragments shorter than    2916 bp
  30% of sequence in fragments shorter than    3383 bp
  35% of sequence in fragments shorter than    3856 bp
  40% of sequence in fragments shorter than    4353 bp
  45% of sequence in fragments shorter than    4877 bp
  50% of sequence in fragments shorter than    5435 bp
  55% of sequence in fragments shorter than    6049 bp
  60% of sequence in fragments shorter than    6708 bp
  65% of sequence in fragments shorter than    7450 bp
  70% of sequence in fragments shorter than    8282 bp
  75% of sequence in fragments shorter than    9263 bp
  80% of sequence in fragments shorter than   10459 bp
  85% of sequence in fragments shorter than   12060 bp
  90% of sequence in fragments shorter than   14388 bp
  95% of sequence in fragments shorter than   18852 bp

# BceAI.HgaI (ACGGC+GACGC) double digest, 1276 thousand fragments (705178+571889 sites), 2334 bp average fragment length
   5% of sequence in fragments shorter than     758 bp
  10% of sequence in fragments shorter than    1283 bp
  15% of sequence in fragments shorter than    1777 bp
  20% of sequence in fragments shorter than    2252 bp
  25% of sequence in fragments shorter than    2741 bp
  30% of sequence in fragments shorter than    3239 bp
  35% of sequence in fragments shorter than    3763 bp
  40% of sequence in fragments shorter than    4318 bp
  45% of sequence in fragments shorter than    4903 bp
  50% of sequence in fragments shorter than    5537 bp
  55% of sequence in fragments shorter than    6231 bp
  60% of sequence in fragments shorter than    7004 bp
  65% of sequence in fragments shorter than    7871 bp
  70% of sequence in fragments shorter than    8857 bp
  75% of sequence in fragments shorter than   10023 bp
  80% of sequence in fragments shorter than   11472 bp
  85% of sequence in fragments shorter than   13372 bp
  90% of sequence in fragments shorter than   16077 bp
  95% of sequence in fragments shorter than   20896 bp

# BceAI.EciI (ACGGC+GGCGGA) double digest, 1276 thousand fragments (705178+571273 sites), 2335 bp average fragment length
   5% of sequence in fragments shorter than     742 bp
  10% of sequence in fragments shorter than    1227 bp
  15% of sequence in fragments shorter than    1676 bp
  20% of sequence in fragments shorter than    2125 bp
  25% of sequence in fragments shorter than    2580 bp
  30% of sequence in fragments shorter than    3057 bp
  35% of sequence in fragments shorter than    3566 bp
  40% of sequence in fragments shorter than    4112 bp
  45% of sequence in fragments shorter than    4704 bp
  50% of sequence in fragments shorter than    5354 bp
  55% of sequence in fragments shorter than    6055 bp
  60% of sequence in fragments shorter than    6845 bp
  65% of sequence in fragments shorter than    7750 bp
  70% of sequence in fragments shorter than    8803 bp
  75% of sequence in fragments shorter than   10065 bp
  80% of sequence in fragments shorter than   11615 bp
  85% of sequence in fragments shorter than   13669 bp
  90% of sequence in fragments shorter than   16678 bp
  95% of sequence in fragments shorter than   22260 bp

# BspHI.SexAI (TCATGA+ACCWGGT) double digest, 1275 thousand fragments (978290+297900 sites), 2335 bp average fragment length
   5% of sequence in fragments shorter than     826 bp
  10% of sequence in fragments shorter than    1253 bp
  15% of sequence in fragments shorter than    1625 bp
  20% of sequence in fragments shorter than    1964 bp
  25% of sequence in fragments shorter than    2283 bp
  30% of sequence in fragments shorter than    2609 bp
  35% of sequence in fragments shorter than    2948 bp
  40% of sequence in fragments shorter than    3291 bp
  45% of sequence in fragments shorter than    3652 bp
  50% of sequence in fragments shorter than    4037 bp
  55% of sequence in fragments shorter than    4435 bp
  60% of sequence in fragments shorter than    4879 bp
  65% of sequence in fragments shorter than    5370 bp
  70% of sequence in fragments shorter than    5932 bp
  75% of sequence in fragments shorter than    6589 bp
  80% of sequence in fragments shorter than    7377 bp
  85% of sequence in fragments shorter than    8394 bp
  90% of sequence in fragments shorter than    9899 bp
  95% of sequence in fragments shorter than   12844 bp

# AccI.AclI (GTMKAC+AACGTT) double digest, 1273 thousand fragments (1101063+172706 sites), 2340 bp average fragment length
   5% of sequence in fragments shorter than     830 bp
  10% of sequence in fragments shorter than    1233 bp
  15% of sequence in fragments shorter than    1593 bp
  20% of sequence in fragments shorter than    1934 bp
  25% of sequence in fragments shorter than    2268 bp
  30% of sequence in fragments shorter than    2607 bp
  35% of sequence in fragments shorter than    2948 bp
  40% of sequence in fragments shorter than    3308 bp
  45% of sequence in fragments shorter than    3670 bp
  50% of sequence in fragments shorter than    4066 bp
  55% of sequence in fragments shorter than    4487 bp
  60% of sequence in fragments shorter than    4939 bp
  65% of sequence in fragments shorter than    5447 bp
  70% of sequence in fragments shorter than    6009 bp
  75% of sequence in fragments shorter than    6667 bp
  80% of sequence in fragments shorter than    7489 bp
  85% of sequence in fragments shorter than    8552 bp
  90% of sequence in fragments shorter than   10075 bp
  95% of sequence in fragments shorter than   12809 bp

# AflII.HaeII (CTTAAG+RGCGCY) double digest, 1272 thousand fragments (643284+630101 sites), 2341 bp average fragment length
   5% of sequence in fragments shorter than     756 bp
  10% of sequence in fragments shorter than    1233 bp
  15% of sequence in fragments shorter than    1659 bp
  20% of sequence in fragments shorter than    2059 bp
  25% of sequence in fragments shorter than    2425 bp
  30% of sequence in fragments shorter than    2811 bp
  35% of sequence in fragments shorter than    3208 bp
  40% of sequence in fragments shorter than    3604 bp
  45% of sequence in fragments shorter than    4015 bp
  50% of sequence in fragments shorter than    4461 bp
  55% of sequence in fragments shorter than    4937 bp
  60% of sequence in fragments shorter than    5458 bp
  65% of sequence in fragments shorter than    6030 bp
  70% of sequence in fragments shorter than    6674 bp
  75% of sequence in fragments shorter than    7424 bp
  80% of sequence in fragments shorter than    8314 bp
  85% of sequence in fragments shorter than    9469 bp
  90% of sequence in fragments shorter than   11072 bp
  95% of sequence in fragments shorter than   13845 bp

# BscGI.PmeI (CCCGT+GTTTAAAC) double digest, 1272 thousand fragments (1231070+42094 sites), 2341 bp average fragment length
   5% of sequence in fragments shorter than     686 bp
  10% of sequence in fragments shorter than    1185 bp
  15% of sequence in fragments shorter than    1664 bp
  20% of sequence in fragments shorter than    2136 bp
  25% of sequence in fragments shorter than    2611 bp
  30% of sequence in fragments shorter than    3103 bp
  35% of sequence in fragments shorter than    3614 bp
  40% of sequence in fragments shorter than    4155 bp
  45% of sequence in fragments shorter than    4727 bp
  50% of sequence in fragments shorter than    5352 bp
  55% of sequence in fragments shorter than    6030 bp
  60% of sequence in fragments shorter than    6775 bp
  65% of sequence in fragments shorter than    7611 bp
  70% of sequence in fragments shorter than    8588 bp
  75% of sequence in fragments shorter than    9750 bp
  80% of sequence in fragments shorter than   11135 bp
  85% of sequence in fragments shorter than   12929 bp
  90% of sequence in fragments shorter than   15469 bp
  95% of sequence in fragments shorter than   19843 bp

# ApaLI.BglII (GTGCAC+AGATCT) double digest, 1270 thousand fragments (496255+774732 sites), 2345 bp average fragment length
   5% of sequence in fragments shorter than     822 bp
  10% of sequence in fragments shorter than    1239 bp
  15% of sequence in fragments shorter than    1596 bp
  20% of sequence in fragments shorter than    1930 bp
  25% of sequence in fragments shorter than    2261 bp
  30% of sequence in fragments shorter than    2589 bp
  35% of sequence in fragments shorter than    2923 bp
  40% of sequence in fragments shorter than    3253 bp
  45% of sequence in fragments shorter than    3609 bp
  50% of sequence in fragments shorter than    3958 bp
  55% of sequence in fragments shorter than    4359 bp
  60% of sequence in fragments shorter than    4797 bp
  65% of sequence in fragments shorter than    5284 bp
  70% of sequence in fragments shorter than    5831 bp
  75% of sequence in fragments shorter than    6479 bp
  80% of sequence in fragments shorter than    7266 bp
  85% of sequence in fragments shorter than    8273 bp
  90% of sequence in fragments shorter than    9708 bp
  95% of sequence in fragments shorter than   12531 bp

# BceAI.MfeI (ACGGC+CAATTG) double digest, 1269 thousand fragments (705178+564303 sites), 2348 bp average fragment length
   5% of sequence in fragments shorter than     809 bp
  10% of sequence in fragments shorter than    1250 bp
  15% of sequence in fragments shorter than    1645 bp
  20% of sequence in fragments shorter than    2009 bp
  25% of sequence in fragments shorter than    2363 bp
  30% of sequence in fragments shorter than    2722 bp
  35% of sequence in fragments shorter than    3089 bp
  40% of sequence in fragments shorter than    3461 bp
  45% of sequence in fragments shorter than    3837 bp
  50% of sequence in fragments shorter than    4239 bp
  55% of sequence in fragments shorter than    4684 bp
  60% of sequence in fragments shorter than    5175 bp
  65% of sequence in fragments shorter than    5713 bp
  70% of sequence in fragments shorter than    6317 bp
  75% of sequence in fragments shorter than    7031 bp
  80% of sequence in fragments shorter than    7888 bp
  85% of sequence in fragments shorter than    8983 bp
  90% of sequence in fragments shorter than   10550 bp
  95% of sequence in fragments shorter than   13288 bp

# BamHI.NdeI (GGATCC+CATATG) double digest, 1268 thousand fragments (365999+903360 sites), 2348 bp average fragment length
   5% of sequence in fragments shorter than     821 bp
  10% of sequence in fragments shorter than    1227 bp
  15% of sequence in fragments shorter than    1616 bp
  20% of sequence in fragments shorter than    1979 bp
  25% of sequence in fragments shorter than    2325 bp
  30% of sequence in fragments shorter than    2683 bp
  35% of sequence in fragments shorter than    3040 bp
  40% of sequence in fragments shorter than    3410 bp
  45% of sequence in fragments shorter than    3796 bp
  50% of sequence in fragments shorter than    4207 bp
  55% of sequence in fragments shorter than    4643 bp
  60% of sequence in fragments shorter than    5110 bp
  65% of sequence in fragments shorter than    5631 bp
  70% of sequence in fragments shorter than    6226 bp
  75% of sequence in fragments shorter than    6925 bp
  80% of sequence in fragments shorter than    7783 bp
  85% of sequence in fragments shorter than    8899 bp
  90% of sequence in fragments shorter than   10496 bp
  95% of sequence in fragments shorter than   13653 bp

# AvaIII.BstEII (ATGCAT+GGTNACC) double digest, 1268 thousand fragments (933357+335865 sites), 2348 bp average fragment length
   5% of sequence in fragments shorter than     835 bp
  10% of sequence in fragments shorter than    1266 bp
  15% of sequence in fragments shorter than    1640 bp
  20% of sequence in fragments shorter than    1990 bp
  25% of sequence in fragments shorter than    2323 bp
  30% of sequence in fragments shorter than    2662 bp
  35% of sequence in fragments shorter than    3007 bp
  40% of sequence in fragments shorter than    3364 bp
  45% of sequence in fragments shorter than    3736 bp
  50% of sequence in fragments shorter than    4130 bp
  55% of sequence in fragments shorter than    4557 bp
  60% of sequence in fragments shorter than    5010 bp
  65% of sequence in fragments shorter than    5523 bp
  70% of sequence in fragments shorter than    6097 bp
  75% of sequence in fragments shorter than    6772 bp
  80% of sequence in fragments shorter than    7602 bp
  85% of sequence in fragments shorter than    8663 bp
  90% of sequence in fragments shorter than   10220 bp
  95% of sequence in fragments shorter than   13276 bp

# BciVI.HgaI (GTATCC+GACGC) double digest, 1267 thousand fragments (696093+571889 sites), 2351 bp average fragment length
   5% of sequence in fragments shorter than     819 bp
  10% of sequence in fragments shorter than    1269 bp
  15% of sequence in fragments shorter than    1668 bp
  20% of sequence in fragments shorter than    2032 bp
  25% of sequence in fragments shorter than    2387 bp
  30% of sequence in fragments shorter than    2749 bp
  35% of sequence in fragments shorter than    3116 bp
  40% of sequence in fragments shorter than    3501 bp
  45% of sequence in fragments shorter than    3906 bp
  50% of sequence in fragments shorter than    4327 bp
  55% of sequence in fragments shorter than    4787 bp
  60% of sequence in fragments shorter than    5288 bp
  65% of sequence in fragments shorter than    5842 bp
  70% of sequence in fragments shorter than    6473 bp
  75% of sequence in fragments shorter than    7208 bp
  80% of sequence in fragments shorter than    8098 bp
  85% of sequence in fragments shorter than    9233 bp
  90% of sequence in fragments shorter than   10843 bp
  95% of sequence in fragments shorter than   13749 bp

# BciVI.EciI (GTATCC+GGCGGA) double digest, 1266 thousand fragments (696093+571273 sites), 2352 bp average fragment length
   5% of sequence in fragments shorter than     815 bp
  10% of sequence in fragments shorter than    1254 bp
  15% of sequence in fragments shorter than    1639 bp
  20% of sequence in fragments shorter than    1986 bp
  25% of sequence in fragments shorter than    2333 bp
  30% of sequence in fragments shorter than    2689 bp
  35% of sequence in fragments shorter than    3052 bp
  40% of sequence in fragments shorter than    3426 bp
  45% of sequence in fragments shorter than    3821 bp
  50% of sequence in fragments shorter than    4234 bp
  55% of sequence in fragments shorter than    4685 bp
  60% of sequence in fragments shorter than    5180 bp
  65% of sequence in fragments shorter than    5729 bp
  70% of sequence in fragments shorter than    6351 bp
  75% of sequence in fragments shorter than    7077 bp
  80% of sequence in fragments shorter than    7962 bp
  85% of sequence in fragments shorter than    9096 bp
  90% of sequence in fragments shorter than   10746 bp
  95% of sequence in fragments shorter than   13805 bp

# BspHI.KpnI (TCATGA+GGTACC) double digest, 1265 thousand fragments (978290+287974 sites), 2354 bp average fragment length
   5% of sequence in fragments shorter than     811 bp
  10% of sequence in fragments shorter than    1250 bp
  15% of sequence in fragments shorter than    1609 bp
  20% of sequence in fragments shorter than    1947 bp
  25% of sequence in fragments shorter than    2272 bp
  30% of sequence in fragments shorter than    2606 bp
  35% of sequence in fragments shorter than    2952 bp
  40% of sequence in fragments shorter than    3307 bp
  45% of sequence in fragments shorter than    3673 bp
  50% of sequence in fragments shorter than    4069 bp
  55% of sequence in fragments shorter than    4493 bp
  60% of sequence in fragments shorter than    4955 bp
  65% of sequence in fragments shorter than    5475 bp
  70% of sequence in fragments shorter than    6063 bp
  75% of sequence in fragments shorter than    6743 bp
  80% of sequence in fragments shorter than    7586 bp
  85% of sequence in fragments shorter than    8676 bp
  90% of sequence in fragments shorter than   10270 bp
  95% of sequence in fragments shorter than   13446 bp

# BspHI.NheI (TCATGA+GCTAGC) double digest, 1265 thousand fragments (978290+287384 sites), 2355 bp average fragment length
   5% of sequence in fragments shorter than     831 bp
  10% of sequence in fragments shorter than    1249 bp
  15% of sequence in fragments shorter than    1620 bp
  20% of sequence in fragments shorter than    1965 bp
  25% of sequence in fragments shorter than    2291 bp
  30% of sequence in fragments shorter than    2633 bp
  35% of sequence in fragments shorter than    2960 bp
  40% of sequence in fragments shorter than    3316 bp
  45% of sequence in fragments shorter than    3693 bp
  50% of sequence in fragments shorter than    4089 bp
  55% of sequence in fragments shorter than    4515 bp
  60% of sequence in fragments shorter than    4988 bp
  65% of sequence in fragments shorter than    5512 bp
  70% of sequence in fragments shorter than    6105 bp
  75% of sequence in fragments shorter than    6801 bp
  80% of sequence in fragments shorter than    7653 bp
  85% of sequence in fragments shorter than    8753 bp
  90% of sequence in fragments shorter than   10369 bp
  95% of sequence in fragments shorter than   13533 bp

# BsaAI.SphI (YACGTR+GCATGC) double digest, 1264 thousand fragments (713801+550952 sites), 2357 bp average fragment length
   5% of sequence in fragments shorter than     842 bp
  10% of sequence in fragments shorter than    1264 bp
  15% of sequence in fragments shorter than    1635 bp
  20% of sequence in fragments shorter than    1976 bp
  25% of sequence in fragments shorter than    2319 bp
  30% of sequence in fragments shorter than    2664 bp
  35% of sequence in fragments shorter than    3009 bp
  40% of sequence in fragments shorter than    3369 bp
  45% of sequence in fragments shorter than    3730 bp
  50% of sequence in fragments shorter than    4137 bp
  55% of sequence in fragments shorter than    4557 bp
  60% of sequence in fragments shorter than    5025 bp
  65% of sequence in fragments shorter than    5544 bp
  70% of sequence in fragments shorter than    6139 bp
  75% of sequence in fragments shorter than    6833 bp
  80% of sequence in fragments shorter than    7666 bp
  85% of sequence in fragments shorter than    8732 bp
  90% of sequence in fragments shorter than   10274 bp
  95% of sequence in fragments shorter than   13105 bp

# BscGI.SalI (CCCGT+GTCGAC) double digest, 1261 thousand fragments (1231070+31396 sites), 2361 bp average fragment length
   5% of sequence in fragments shorter than     687 bp
  10% of sequence in fragments shorter than    1194 bp
  15% of sequence in fragments shorter than    1683 bp
  20% of sequence in fragments shorter than    2165 bp
  25% of sequence in fragments shorter than    2652 bp
  30% of sequence in fragments shorter than    3160 bp
  35% of sequence in fragments shorter than    3684 bp
  40% of sequence in fragments shorter than    4244 bp
  45% of sequence in fragments shorter than    4838 bp
  50% of sequence in fragments shorter than    5487 bp
  55% of sequence in fragments shorter than    6194 bp
  60% of sequence in fragments shorter than    6971 bp
  65% of sequence in fragments shorter than    7851 bp
  70% of sequence in fragments shorter than    8880 bp
  75% of sequence in fragments shorter than   10078 bp
  80% of sequence in fragments shorter than   11564 bp
  85% of sequence in fragments shorter than   13476 bp
  90% of sequence in fragments shorter than   16184 bp
  95% of sequence in fragments shorter than   20734 bp

# BstEII.StuI (GGTNACC+AGGCCT) double digest, 1261 thousand fragments (335865+925729 sites), 2362 bp average fragment length
   5% of sequence in fragments shorter than     706 bp
  10% of sequence in fragments shorter than    1198 bp
  15% of sequence in fragments shorter than    1625 bp
  20% of sequence in fragments shorter than    2027 bp
  25% of sequence in fragments shorter than    2451 bp
  30% of sequence in fragments shorter than    2882 bp
  35% of sequence in fragments shorter than    3315 bp
  40% of sequence in fragments shorter than    3771 bp
  45% of sequence in fragments shorter than    4248 bp
  50% of sequence in fragments shorter than    4754 bp
  55% of sequence in fragments shorter than    5304 bp
  60% of sequence in fragments shorter than    5897 bp
  65% of sequence in fragments shorter than    6563 bp
  70% of sequence in fragments shorter than    7324 bp
  75% of sequence in fragments shorter than    8196 bp
  80% of sequence in fragments shorter than    9244 bp
  85% of sequence in fragments shorter than   10591 bp
  90% of sequence in fragments shorter than   12504 bp
  95% of sequence in fragments shorter than   15769 bp

# HindIII.SpeI (AAGCTT+ACTAGT) double digest, 1260 thousand fragments (860365+400286 sites), 2364 bp average fragment length
   5% of sequence in fragments shorter than     829 bp
  10% of sequence in fragments shorter than    1252 bp
  15% of sequence in fragments shorter than    1616 bp
  20% of sequence in fragments shorter than    1916 bp
  25% of sequence in fragments shorter than    2235 bp
  30% of sequence in fragments shorter than    2574 bp
  35% of sequence in fragments shorter than    2920 bp
  40% of sequence in fragments shorter than    3276 bp
  45% of sequence in fragments shorter than    3655 bp
  50% of sequence in fragments shorter than    4050 bp
  55% of sequence in fragments shorter than    4479 bp
  60% of sequence in fragments shorter than    4951 bp
  65% of sequence in fragments shorter than    5477 bp
  70% of sequence in fragments shorter than    6087 bp
  75% of sequence in fragments shorter than    6814 bp
  80% of sequence in fragments shorter than    7700 bp
  85% of sequence in fragments shorter than    8862 bp
  90% of sequence in fragments shorter than   10602 bp
  95% of sequence in fragments shorter than   14089 bp

# AccI.PacI (GTMKAC+TTAATTAA) double digest, 1260 thousand fragments (1101063+159455 sites), 2364 bp average fragment length
   5% of sequence in fragments shorter than     838 bp
  10% of sequence in fragments shorter than    1265 bp
  15% of sequence in fragments shorter than    1635 bp
  20% of sequence in fragments shorter than    1982 bp
  25% of sequence in fragments shorter than    2325 bp
  30% of sequence in fragments shorter than    2668 bp
  35% of sequence in fragments shorter than    3014 bp
  40% of sequence in fragments shorter than    3381 bp
  45% of sequence in fragments shorter than    3759 bp
  50% of sequence in fragments shorter than    4165 bp
  55% of sequence in fragments shorter than    4601 bp
  60% of sequence in fragments shorter than    5079 bp
  65% of sequence in fragments shorter than    5600 bp
  70% of sequence in fragments shorter than    6139 bp
  75% of sequence in fragments shorter than    6847 bp
  80% of sequence in fragments shorter than    7695 bp
  85% of sequence in fragments shorter than    8814 bp
  90% of sequence in fragments shorter than   10431 bp
  95% of sequence in fragments shorter than   13461 bp

# BciVI.MfeI (GTATCC+CAATTG) double digest, 1259 thousand fragments (696093+564303 sites), 2365 bp average fragment length
   5% of sequence in fragments shorter than     833 bp
  10% of sequence in fragments shorter than    1259 bp
  15% of sequence in fragments shorter than    1636 bp
  20% of sequence in fragments shorter than    1972 bp
  25% of sequence in fragments shorter than    2306 bp
  30% of sequence in fragments shorter than    2650 bp
  35% of sequence in fragments shorter than    2998 bp
  40% of sequence in fragments shorter than    3355 bp
  45% of sequence in fragments shorter than    3734 bp
  50% of sequence in fragments shorter than    4137 bp
  55% of sequence in fragments shorter than    4565 bp
  60% of sequence in fragments shorter than    5035 bp
  65% of sequence in fragments shorter than    5564 bp
  70% of sequence in fragments shorter than    6167 bp
  75% of sequence in fragments shorter than    6871 bp
  80% of sequence in fragments shorter than    7730 bp
  85% of sequence in fragments shorter than    8860 bp
  90% of sequence in fragments shorter than   10519 bp
  95% of sequence in fragments shorter than   13637 bp

# AccI.Hpy99I (GTMKAC+CGWCG) double digest, 1258 thousand fragments (1101063+157917 sites), 2367 bp average fragment length
   5% of sequence in fragments shorter than     846 bp
  10% of sequence in fragments shorter than    1284 bp
  15% of sequence in fragments shorter than    1661 bp
  20% of sequence in fragments shorter than    2010 bp
  25% of sequence in fragments shorter than    2358 bp
  30% of sequence in fragments shorter than    2702 bp
  35% of sequence in fragments shorter than    3050 bp
  40% of sequence in fragments shorter than    3416 bp
  45% of sequence in fragments shorter than    3792 bp
  50% of sequence in fragments shorter than    4193 bp
  55% of sequence in fragments shorter than    4622 bp
  60% of sequence in fragments shorter than    5084 bp
  65% of sequence in fragments shorter than    5591 bp
  70% of sequence in fragments shorter than    6115 bp
  75% of sequence in fragments shorter than    6775 bp
  80% of sequence in fragments shorter than    7572 bp
  85% of sequence in fragments shorter than    8607 bp
  90% of sequence in fragments shorter than   10102 bp
  95% of sequence in fragments shorter than   12709 bp

# AclI.PpuMI (AACGTT+RGGWCCY) double digest, 1257 thousand fragments (172706+1085138 sites), 2369 bp average fragment length
   5% of sequence in fragments shorter than     777 bp
  10% of sequence in fragments shorter than    1236 bp
  15% of sequence in fragments shorter than    1660 bp
  20% of sequence in fragments shorter than    2064 bp
  25% of sequence in fragments shorter than    2468 bp
  30% of sequence in fragments shorter than    2874 bp
  35% of sequence in fragments shorter than    3289 bp
  40% of sequence in fragments shorter than    3709 bp
  45% of sequence in fragments shorter than    4168 bp
  50% of sequence in fragments shorter than    4676 bp
  55% of sequence in fragments shorter than    5228 bp
  60% of sequence in fragments shorter than    5834 bp
  65% of sequence in fragments shorter than    6519 bp
  70% of sequence in fragments shorter than    7295 bp
  75% of sequence in fragments shorter than    8197 bp
  80% of sequence in fragments shorter than    9303 bp
  85% of sequence in fragments shorter than   10750 bp
  90% of sequence in fragments shorter than   12870 bp
  95% of sequence in fragments shorter than   16853 bp

# BsaAI.ScaI (YACGTR+AGTACT) double digest, 1257 thousand fragments (713801+543793 sites), 2370 bp average fragment length
   5% of sequence in fragments shorter than     854 bp
  10% of sequence in fragments shorter than    1274 bp
  15% of sequence in fragments shorter than    1640 bp
  20% of sequence in fragments shorter than    1977 bp
  25% of sequence in fragments shorter than    2316 bp
  30% of sequence in fragments shorter than    2653 bp
  35% of sequence in fragments shorter than    2993 bp
  40% of sequence in fragments shorter than    3343 bp
  45% of sequence in fragments shorter than    3700 bp
  50% of sequence in fragments shorter than    4090 bp
  55% of sequence in fragments shorter than    4497 bp
  60% of sequence in fragments shorter than    4953 bp
  65% of sequence in fragments shorter than    5451 bp
  70% of sequence in fragments shorter than    6018 bp
  75% of sequence in fragments shorter than    6669 bp
  80% of sequence in fragments shorter than    7464 bp
  85% of sequence in fragments shorter than    8489 bp
  90% of sequence in fragments shorter than    9912 bp
  95% of sequence in fragments shorter than   12546 bp

# AclI.PvuII (AACGTT+CAGCTG) double digest, 1256 thousand fragments (172706+1084260 sites), 2371 bp average fragment length
   5% of sequence in fragments shorter than     809 bp
  10% of sequence in fragments shorter than    1242 bp
  15% of sequence in fragments shorter than    1635 bp
  20% of sequence in fragments shorter than    2010 bp
  25% of sequence in fragments shorter than    2383 bp
  30% of sequence in fragments shorter than    2756 bp
  35% of sequence in fragments shorter than    3138 bp
  40% of sequence in fragments shorter than    3537 bp
  45% of sequence in fragments shorter than    3965 bp
  50% of sequence in fragments shorter than    4418 bp
  55% of sequence in fragments shorter than    4920 bp
  60% of sequence in fragments shorter than    5469 bp
  65% of sequence in fragments shorter than    6076 bp
  70% of sequence in fragments shorter than    6765 bp
  75% of sequence in fragments shorter than    7568 bp
  80% of sequence in fragments shorter than    8549 bp
  85% of sequence in fragments shorter than    9833 bp
  90% of sequence in fragments shorter than   11674 bp
  95% of sequence in fragments shorter than   15189 bp

# BceAI.SphI (ACGGC+GCATGC) double digest, 1255 thousand fragments (705178+550952 sites), 2373 bp average fragment length
   5% of sequence in fragments shorter than     795 bp
  10% of sequence in fragments shorter than    1249 bp
  15% of sequence in fragments shorter than    1656 bp
  20% of sequence in fragments shorter than    2047 bp
  25% of sequence in fragments shorter than    2441 bp
  30% of sequence in fragments shorter than    2842 bp
  35% of sequence in fragments shorter than    3254 bp
  40% of sequence in fragments shorter than    3689 bp
  45% of sequence in fragments shorter than    4152 bp
  50% of sequence in fragments shorter than    4651 bp
  55% of sequence in fragments shorter than    5191 bp
  60% of sequence in fragments shorter than    5784 bp
  65% of sequence in fragments shorter than    6447 bp
  70% of sequence in fragments shorter than    7205 bp
  75% of sequence in fragments shorter than    8102 bp
  80% of sequence in fragments shorter than    9182 bp
  85% of sequence in fragments shorter than   10604 bp
  90% of sequence in fragments shorter than   12646 bp
  95% of sequence in fragments shorter than   16385 bp

# ApaLI.NcoI (GTGCAC+CCATGG) double digest, 1255 thousand fragments (496255+759594 sites), 2373 bp average fragment length
   5% of sequence in fragments shorter than     813 bp
  10% of sequence in fragments shorter than    1265 bp
  15% of sequence in fragments shorter than    1654 bp
  20% of sequence in fragments shorter than    2027 bp
  25% of sequence in fragments shorter than    2393 bp
  30% of sequence in fragments shorter than    2761 bp
  35% of sequence in fragments shorter than    3137 bp
  40% of sequence in fragments shorter than    3525 bp
  45% of sequence in fragments shorter than    3941 bp
  50% of sequence in fragments shorter than    4379 bp
  55% of sequence in fragments shorter than    4831 bp
  60% of sequence in fragments shorter than    5343 bp
  65% of sequence in fragments shorter than    5873 bp
  70% of sequence in fragments shorter than    6508 bp
  75% of sequence in fragments shorter than    7272 bp
  80% of sequence in fragments shorter than    8215 bp
  85% of sequence in fragments shorter than    9444 bp
  90% of sequence in fragments shorter than   11200 bp
  95% of sequence in fragments shorter than   14641 bp

# BscGI.MluI (CCCGT+ACGCGT) double digest, 1252 thousand fragments (1231070+21415 sites), 2380 bp average fragment length
   5% of sequence in fragments shorter than     688 bp
  10% of sequence in fragments shorter than    1203 bp
  15% of sequence in fragments shorter than    1700 bp
  20% of sequence in fragments shorter than    2191 bp
  25% of sequence in fragments shorter than    2689 bp
  30% of sequence in fragments shorter than    3207 bp
  35% of sequence in fragments shorter than    3742 bp
  40% of sequence in fragments shorter than    4313 bp
  45% of sequence in fragments shorter than    4919 bp
  50% of sequence in fragments shorter than    5585 bp
  55% of sequence in fragments shorter than    6308 bp
  60% of sequence in fragments shorter than    7104 bp
  65% of sequence in fragments shorter than    8011 bp
  70% of sequence in fragments shorter than    9076 bp
  75% of sequence in fragments shorter than   10306 bp
  80% of sequence in fragments shorter than   11822 bp
  85% of sequence in fragments shorter than   13780 bp
  90% of sequence in fragments shorter than   16537 bp
  95% of sequence in fragments shorter than   21249 bp

# SpeI.XbaI (ACTAGT+TCTAGA) double digest, 1250 thousand fragments (400286+850998 sites), 2382 bp average fragment length
   5% of sequence in fragments shorter than     842 bp
  10% of sequence in fragments shorter than    1261 bp
  15% of sequence in fragments shorter than    1614 bp
  20% of sequence in fragments shorter than    1975 bp
  25% of sequence in fragments shorter than    2317 bp
  30% of sequence in fragments shorter than    2675 bp
  35% of sequence in fragments shorter than    3041 bp
  40% of sequence in fragments shorter than    3422 bp
  45% of sequence in fragments shorter than    3813 bp
  50% of sequence in fragments shorter than    4230 bp
  55% of sequence in fragments shorter than    4680 bp
  60% of sequence in fragments shorter than    5173 bp
  65% of sequence in fragments shorter than    5719 bp
  70% of sequence in fragments shorter than    6353 bp
  75% of sequence in fragments shorter than    7097 bp
  80% of sequence in fragments shorter than    8011 bp
  85% of sequence in fragments shorter than    9213 bp
  90% of sequence in fragments shorter than   10984 bp
  95% of sequence in fragments shorter than   14381 bp

# HindIII.HpaI (AAGCTT+GTTAAC) double digest, 1249 thousand fragments (860365+389418 sites), 2385 bp average fragment length
   5% of sequence in fragments shorter than     824 bp
  10% of sequence in fragments shorter than    1242 bp
  15% of sequence in fragments shorter than    1619 bp
  20% of sequence in fragments shorter than    1956 bp
  25% of sequence in fragments shorter than    2305 bp
  30% of sequence in fragments shorter than    2649 bp
  35% of sequence in fragments shorter than    2993 bp
  40% of sequence in fragments shorter than    3354 bp
  45% of sequence in fragments shorter than    3734 bp
  50% of sequence in fragments shorter than    4134 bp
  55% of sequence in fragments shorter than    4566 bp
  60% of sequence in fragments shorter than    5047 bp
  65% of sequence in fragments shorter than    5575 bp
  70% of sequence in fragments shorter than    6183 bp
  75% of sequence in fragments shorter than    6904 bp
  80% of sequence in fragments shorter than    7794 bp
  85% of sequence in fragments shorter than    8949 bp
  90% of sequence in fragments shorter than   10698 bp
  95% of sequence in fragments shorter than   14244 bp

# BceAI.ScaI (ACGGC+AGTACT) double digest, 1248 thousand fragments (705178+543793 sites), 2386 bp average fragment length
   5% of sequence in fragments shorter than     823 bp
  10% of sequence in fragments shorter than    1272 bp
  15% of sequence in fragments shorter than    1665 bp
  20% of sequence in fragments shorter than    2035 bp
  25% of sequence in fragments shorter than    2407 bp
  30% of sequence in fragments shorter than    2775 bp
  35% of sequence in fragments shorter than    3151 bp
  40% of sequence in fragments shorter than    3547 bp
  45% of sequence in fragments shorter than    3960 bp
  50% of sequence in fragments shorter than    4401 bp
  55% of sequence in fragments shorter than    4872 bp
  60% of sequence in fragments shorter than    5388 bp
  65% of sequence in fragments shorter than    5955 bp
  70% of sequence in fragments shorter than    6603 bp
  75% of sequence in fragments shorter than    7364 bp
  80% of sequence in fragments shorter than    8274 bp
  85% of sequence in fragments shorter than    9445 bp
  90% of sequence in fragments shorter than   11159 bp
  95% of sequence in fragments shorter than   14317 bp

# EcoRI.SpeI (GAATTC+ACTAGT) double digest, 1247 thousand fragments (847401+400286 sites), 2389 bp average fragment length
   5% of sequence in fragments shorter than     801 bp
  10% of sequence in fragments shorter than    1255 bp
  15% of sequence in fragments shorter than    1648 bp
  20% of sequence in fragments shorter than    2013 bp
  25% of sequence in fragments shorter than    2348 bp
  30% of sequence in fragments shorter than    2703 bp
  35% of sequence in fragments shorter than    3063 bp
  40% of sequence in fragments shorter than    3433 bp
  45% of sequence in fragments shorter than    3825 bp
  50% of sequence in fragments shorter than    4240 bp
  55% of sequence in fragments shorter than    4689 bp
  60% of sequence in fragments shorter than    5180 bp
  65% of sequence in fragments shorter than    5720 bp
  70% of sequence in fragments shorter than    6341 bp
  75% of sequence in fragments shorter than    7070 bp
  80% of sequence in fragments shorter than    7962 bp
  85% of sequence in fragments shorter than    9143 bp
  90% of sequence in fragments shorter than   10838 bp
  95% of sequence in fragments shorter than   14081 bp

# BciVI.SphI (GTATCC+GCATGC) double digest, 1246 thousand fragments (696093+550952 sites), 2390 bp average fragment length
   5% of sequence in fragments shorter than     853 bp
  10% of sequence in fragments shorter than    1282 bp
  15% of sequence in fragments shorter than    1652 bp
  20% of sequence in fragments shorter than    1978 bp
  25% of sequence in fragments shorter than    2304 bp
  30% of sequence in fragments shorter than    2635 bp
  35% of sequence in fragments shorter than    2975 bp
  40% of sequence in fragments shorter than    3320 bp
  45% of sequence in fragments shorter than    3682 bp
  50% of sequence in fragments shorter than    4061 bp
  55% of sequence in fragments shorter than    4468 bp
  60% of sequence in fragments shorter than    4911 bp
  65% of sequence in fragments shorter than    5407 bp
  70% of sequence in fragments shorter than    5959 bp
  75% of sequence in fragments shorter than    6600 bp
  80% of sequence in fragments shorter than    7376 bp
  85% of sequence in fragments shorter than    8372 bp
  90% of sequence in fragments shorter than    9794 bp
  95% of sequence in fragments shorter than   12427 bp

# AflII.SacI (CTTAAG+GAGCTC) double digest, 1246 thousand fragments (643284+603323 sites), 2391 bp average fragment length
   5% of sequence in fragments shorter than     843 bp
  10% of sequence in fragments shorter than    1275 bp
  15% of sequence in fragments shorter than    1645 bp
  20% of sequence in fragments shorter than    1994 bp
  25% of sequence in fragments shorter than    2318 bp
  30% of sequence in fragments shorter than    2626 bp
  35% of sequence in fragments shorter than    2968 bp
  40% of sequence in fragments shorter than    3320 bp
  45% of sequence in fragments shorter than    3684 bp
  50% of sequence in fragments shorter than    4072 bp
  55% of sequence in fragments shorter than    4487 bp
  60% of sequence in fragments shorter than    4940 bp
  65% of sequence in fragments shorter than    5445 bp
  70% of sequence in fragments shorter than    6013 bp
  75% of sequence in fragments shorter than    6681 bp
  80% of sequence in fragments shorter than    7499 bp
  85% of sequence in fragments shorter than    8515 bp
  90% of sequence in fragments shorter than   10031 bp
  95% of sequence in fragments shorter than   12949 bp

# BscGI.SgrAI (CCCGT+CRCCGGYG) double digest, 1245 thousand fragments (1231070+15270 sites), 2391 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1210 bp
  15% of sequence in fragments shorter than    1713 bp
  20% of sequence in fragments shorter than    2208 bp
  25% of sequence in fragments shorter than    2709 bp
  30% of sequence in fragments shorter than    3231 bp
  35% of sequence in fragments shorter than    3771 bp
  40% of sequence in fragments shorter than    4345 bp
  45% of sequence in fragments shorter than    4958 bp
  50% of sequence in fragments shorter than    5627 bp
  55% of sequence in fragments shorter than    6353 bp
  60% of sequence in fragments shorter than    7157 bp
  65% of sequence in fragments shorter than    8073 bp
  70% of sequence in fragments shorter than    9144 bp
  75% of sequence in fragments shorter than   10390 bp
  80% of sequence in fragments shorter than   11921 bp
  85% of sequence in fragments shorter than   13894 bp
  90% of sequence in fragments shorter than   16669 bp
  95% of sequence in fragments shorter than   21436 bp

# BscGI.NruI (CCCGT+TCGCGA) double digest, 1245 thousand fragments (1231070+14872 sites), 2392 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1210 bp
  15% of sequence in fragments shorter than    1711 bp
  20% of sequence in fragments shorter than    2204 bp
  25% of sequence in fragments shorter than    2704 bp
  30% of sequence in fragments shorter than    3225 bp
  35% of sequence in fragments shorter than    3763 bp
  40% of sequence in fragments shorter than    4335 bp
  45% of sequence in fragments shorter than    4946 bp
  50% of sequence in fragments shorter than    5612 bp
  55% of sequence in fragments shorter than    6337 bp
  60% of sequence in fragments shorter than    7137 bp
  65% of sequence in fragments shorter than    8048 bp
  70% of sequence in fragments shorter than    9121 bp
  75% of sequence in fragments shorter than   10361 bp
  80% of sequence in fragments shorter than   11880 bp
  85% of sequence in fragments shorter than   13841 bp
  90% of sequence in fragments shorter than   16606 bp
  95% of sequence in fragments shorter than   21371 bp

# BscGI.PvuI (CCCGT+CGATCG) double digest, 1244 thousand fragments (1231070+14186 sites), 2393 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1210 bp
  15% of sequence in fragments shorter than    1711 bp
  20% of sequence in fragments shorter than    2206 bp
  25% of sequence in fragments shorter than    2705 bp
  30% of sequence in fragments shorter than    3226 bp
  35% of sequence in fragments shorter than    3763 bp
  40% of sequence in fragments shorter than    4335 bp
  45% of sequence in fragments shorter than    4945 bp
  50% of sequence in fragments shorter than    5610 bp
  55% of sequence in fragments shorter than    6336 bp
  60% of sequence in fragments shorter than    7136 bp
  65% of sequence in fragments shorter than    8048 bp
  70% of sequence in fragments shorter than    9120 bp
  75% of sequence in fragments shorter than   10361 bp
  80% of sequence in fragments shorter than   11880 bp
  85% of sequence in fragments shorter than   13849 bp
  90% of sequence in fragments shorter than   16613 bp
  95% of sequence in fragments shorter than   21366 bp

# BscGI.FseI (CCCGT+GGCCGGCC) double digest, 1244 thousand fragments (1231070+13662 sites), 2394 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1210 bp
  15% of sequence in fragments shorter than    1714 bp
  20% of sequence in fragments shorter than    2210 bp
  25% of sequence in fragments shorter than    2713 bp
  30% of sequence in fragments shorter than    3237 bp
  35% of sequence in fragments shorter than    3778 bp
  40% of sequence in fragments shorter than    4353 bp
  45% of sequence in fragments shorter than    4968 bp
  50% of sequence in fragments shorter than    5637 bp
  55% of sequence in fragments shorter than    6365 bp
  60% of sequence in fragments shorter than    7174 bp
  65% of sequence in fragments shorter than    8090 bp
  70% of sequence in fragments shorter than    9164 bp
  75% of sequence in fragments shorter than   10414 bp
  80% of sequence in fragments shorter than   11951 bp
  85% of sequence in fragments shorter than   13928 bp
  90% of sequence in fragments shorter than   16709 bp
  95% of sequence in fragments shorter than   21511 bp

# PacI.PpuMI (TTAATTAA+RGGWCCY) double digest, 1244 thousand fragments (159455+1085138 sites), 2395 bp average fragment length
   5% of sequence in fragments shorter than     803 bp
  10% of sequence in fragments shorter than    1277 bp
  15% of sequence in fragments shorter than    1704 bp
  20% of sequence in fragments shorter than    2104 bp
  25% of sequence in fragments shorter than    2512 bp
  30% of sequence in fragments shorter than    2924 bp
  35% of sequence in fragments shorter than    3340 bp
  40% of sequence in fragments shorter than    3742 bp
  45% of sequence in fragments shorter than    4195 bp
  50% of sequence in fragments shorter than    4697 bp
  55% of sequence in fragments shorter than    5238 bp
  60% of sequence in fragments shorter than    5835 bp
  65% of sequence in fragments shorter than    6507 bp
  70% of sequence in fragments shorter than    7275 bp
  75% of sequence in fragments shorter than    8178 bp
  80% of sequence in fragments shorter than    9287 bp
  85% of sequence in fragments shorter than   10727 bp
  90% of sequence in fragments shorter than   12899 bp
  95% of sequence in fragments shorter than   17381 bp

# PacI.PvuII (TTAATTAA+CAGCTG) double digest, 1243 thousand fragments (159455+1084260 sites), 2396 bp average fragment length
   5% of sequence in fragments shorter than     826 bp
  10% of sequence in fragments shorter than    1272 bp
  15% of sequence in fragments shorter than    1666 bp
  20% of sequence in fragments shorter than    2047 bp
  25% of sequence in fragments shorter than    2423 bp
  30% of sequence in fragments shorter than    2796 bp
  35% of sequence in fragments shorter than    3183 bp
  40% of sequence in fragments shorter than    3590 bp
  45% of sequence in fragments shorter than    4023 bp
  50% of sequence in fragments shorter than    4489 bp
  55% of sequence in fragments shorter than    4987 bp
  60% of sequence in fragments shorter than    5538 bp
  65% of sequence in fragments shorter than    6148 bp
  70% of sequence in fragments shorter than    6830 bp
  75% of sequence in fragments shorter than    7636 bp
  80% of sequence in fragments shorter than    8620 bp
  85% of sequence in fragments shorter than    9909 bp
  90% of sequence in fragments shorter than   11805 bp
  95% of sequence in fragments shorter than   15606 bp

# Hpy99I.PpuMI (CGWCG+RGGWCCY) double digest, 1242 thousand fragments (157917+1085138 sites), 2398 bp average fragment length
   5% of sequence in fragments shorter than     769 bp
  10% of sequence in fragments shorter than    1274 bp
  15% of sequence in fragments shorter than    1743 bp
  20% of sequence in fragments shorter than    2195 bp
  25% of sequence in fragments shorter than    2652 bp
  30% of sequence in fragments shorter than    3119 bp
  35% of sequence in fragments shorter than    3571 bp
  40% of sequence in fragments shorter than    4055 bp
  45% of sequence in fragments shorter than    4594 bp
  50% of sequence in fragments shorter than    5181 bp
  55% of sequence in fragments shorter than    5822 bp
  60% of sequence in fragments shorter than    6538 bp
  65% of sequence in fragments shorter than    7339 bp
  70% of sequence in fragments shorter than    8246 bp
  75% of sequence in fragments shorter than    9347 bp
  80% of sequence in fragments shorter than   10693 bp
  85% of sequence in fragments shorter than   12435 bp
  90% of sequence in fragments shorter than   15059 bp
  95% of sequence in fragments shorter than   20292 bp

# Hpy99I.PvuII (CGWCG+CAGCTG) double digest, 1241 thousand fragments (157917+1084260 sites), 2399 bp average fragment length
   5% of sequence in fragments shorter than     798 bp
  10% of sequence in fragments shorter than    1261 bp
  15% of sequence in fragments shorter than    1687 bp
  20% of sequence in fragments shorter than    2101 bp
  25% of sequence in fragments shorter than    2513 bp
  30% of sequence in fragments shorter than    2922 bp
  35% of sequence in fragments shorter than    3356 bp
  40% of sequence in fragments shorter than    3815 bp
  45% of sequence in fragments shorter than    4307 bp
  50% of sequence in fragments shorter than    4833 bp
  55% of sequence in fragments shorter than    5412 bp
  60% of sequence in fragments shorter than    6043 bp
  65% of sequence in fragments shorter than    6744 bp
  70% of sequence in fragments shorter than    7539 bp
  75% of sequence in fragments shorter than    8486 bp
  80% of sequence in fragments shorter than    9655 bp
  85% of sequence in fragments shorter than   11202 bp
  90% of sequence in fragments shorter than   13444 bp
  95% of sequence in fragments shorter than   17829 bp

# AvaI.BsrBI (CYCGRG+CCGCTC) double digest, 1240 thousand fragments (1005714+235523 sites), 2401 bp average fragment length
   5% of sequence in fragments shorter than     777 bp
  10% of sequence in fragments shorter than    1294 bp
  15% of sequence in fragments shorter than    1798 bp
  20% of sequence in fragments shorter than    2307 bp
  25% of sequence in fragments shorter than    2844 bp
  30% of sequence in fragments shorter than    3416 bp
  35% of sequence in fragments shorter than    4030 bp
  40% of sequence in fragments shorter than    4689 bp
  45% of sequence in fragments shorter than    5408 bp
  50% of sequence in fragments shorter than    6207 bp
  55% of sequence in fragments shorter than    7092 bp
  60% of sequence in fragments shorter than    8094 bp
  65% of sequence in fragments shorter than    9245 bp
  70% of sequence in fragments shorter than   10559 bp
  75% of sequence in fragments shorter than   12153 bp
  80% of sequence in fragments shorter than   14164 bp
  85% of sequence in fragments shorter than   16744 bp
  90% of sequence in fragments shorter than   20591 bp
  95% of sequence in fragments shorter than   28125 bp

# BscGI.NotI (CCCGT+GCGGCCGC) double digest, 1240 thousand fragments (1231070+9795 sites), 2402 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1215 bp
  15% of sequence in fragments shorter than    1723 bp
  20% of sequence in fragments shorter than    2221 bp
  25% of sequence in fragments shorter than    2725 bp
  30% of sequence in fragments shorter than    3250 bp
  35% of sequence in fragments shorter than    3793 bp
  40% of sequence in fragments shorter than    4369 bp
  45% of sequence in fragments shorter than    4986 bp
  50% of sequence in fragments shorter than    5660 bp
  55% of sequence in fragments shorter than    6388 bp
  60% of sequence in fragments shorter than    7197 bp
  65% of sequence in fragments shorter than    8118 bp
  70% of sequence in fragments shorter than    9196 bp
  75% of sequence in fragments shorter than   10446 bp
  80% of sequence in fragments shorter than   11986 bp
  85% of sequence in fragments shorter than   13967 bp
  90% of sequence in fragments shorter than   16751 bp
  95% of sequence in fragments shorter than   21563 bp

# BscGI.RsrII (CCCGT+CGGWCCG) double digest, 1240 thousand fragments (1231070+9764 sites), 2402 bp average fragment length
   5% of sequence in fragments shorter than     689 bp
  10% of sequence in fragments shorter than    1213 bp
  15% of sequence in fragments shorter than    1720 bp
  20% of sequence in fragments shorter than    2217 bp
  25% of sequence in fragments shorter than    2719 bp
  30% of sequence in fragments shorter than    3243 bp
  35% of sequence in fragments shorter than    3785 bp
  40% of sequence in fragments shorter than    4360 bp
  45% of sequence in fragments shorter than    4975 bp
  50% of sequence in fragments shorter than    5645 bp
  55% of sequence in fragments shorter than    6374 bp
  60% of sequence in fragments shorter than    7180 bp
  65% of sequence in fragments shorter than    8099 bp
  70% of sequence in fragments shorter than    9177 bp
  75% of sequence in fragments shorter than   10425 bp
  80% of sequence in fragments shorter than   11964 bp
  85% of sequence in fragments shorter than   13943 bp
  90% of sequence in fragments shorter than   16729 bp
  95% of sequence in fragments shorter than   21526 bp

# HpaI.XbaI (GTTAAC+TCTAGA) double digest, 1239 thousand fragments (389418+850998 sites), 2403 bp average fragment length
   5% of sequence in fragments shorter than     837 bp
  10% of sequence in fragments shorter than    1250 bp
  15% of sequence in fragments shorter than    1609 bp
  20% of sequence in fragments shorter than    1958 bp
  25% of sequence in fragments shorter than    2295 bp
  30% of sequence in fragments shorter than    2651 bp
  35% of sequence in fragments shorter than    3008 bp
  40% of sequence in fragments shorter than    3376 bp
  45% of sequence in fragments shorter than    3762 bp
  50% of sequence in fragments shorter than    4172 bp
  55% of sequence in fragments shorter than    4610 bp
  60% of sequence in fragments shorter than    5092 bp
  65% of sequence in fragments shorter than    5632 bp
  70% of sequence in fragments shorter than    6247 bp
  75% of sequence in fragments shorter than    6972 bp
  80% of sequence in fragments shorter than    7855 bp
  85% of sequence in fragments shorter than    9005 bp
  90% of sequence in fragments shorter than   10688 bp
  95% of sequence in fragments shorter than   13869 bp

# BciVI.ScaI (GTATCC+AGTACT) double digest, 1239 thousand fragments (696093+543793 sites), 2404 bp average fragment length
   5% of sequence in fragments shorter than     850 bp
  10% of sequence in fragments shorter than    1284 bp
  15% of sequence in fragments shorter than    1657 bp
  20% of sequence in fragments shorter than    1990 bp
  25% of sequence in fragments shorter than    2327 bp
  30% of sequence in fragments shorter than    2669 bp
  35% of sequence in fragments shorter than    3017 bp
  40% of sequence in fragments shorter than    3375 bp
  45% of sequence in fragments shorter than    3753 bp
  50% of sequence in fragments shorter than    4154 bp
  55% of sequence in fragments shorter than    4582 bp
  60% of sequence in fragments shorter than    5050 bp
  65% of sequence in fragments shorter than    5577 bp
  70% of sequence in fragments shorter than    6174 bp
  75% of sequence in fragments shorter than    6881 bp
  80% of sequence in fragments shorter than    7741 bp
  85% of sequence in fragments shorter than    8868 bp
  90% of sequence in fragments shorter than   10515 bp
  95% of sequence in fragments shorter than   13770 bp

# BstEII.NdeI (GGTNACC+CATATG) double digest, 1238 thousand fragments (335865+903360 sites), 2405 bp average fragment length
   5% of sequence in fragments shorter than     828 bp
  10% of sequence in fragments shorter than    1248 bp
  15% of sequence in fragments shorter than    1642 bp
  20% of sequence in fragments shorter than    2009 bp
  25% of sequence in fragments shorter than    2357 bp
  30% of sequence in fragments shorter than    2715 bp
  35% of sequence in fragments shorter than    3045 bp
  40% of sequence in fragments shorter than    3413 bp
  45% of sequence in fragments shorter than    3805 bp
  50% of sequence in fragments shorter than    4219 bp
  55% of sequence in fragments shorter than    4670 bp
  60% of sequence in fragments shorter than    5157 bp
  65% of sequence in fragments shorter than    5703 bp
  70% of sequence in fragments shorter than    6322 bp
  75% of sequence in fragments shorter than    7046 bp
  80% of sequence in fragments shorter than    7941 bp
  85% of sequence in fragments shorter than    9110 bp
  90% of sequence in fragments shorter than   10793 bp
  95% of sequence in fragments shorter than   14293 bp

# AflII.AvrII (CTTAAG+CCTAGG) double digest, 1237 thousand fragments (643284+594956 sites), 2407 bp average fragment length
   5% of sequence in fragments shorter than     830 bp
  10% of sequence in fragments shorter than    1283 bp
  15% of sequence in fragments shorter than    1662 bp
  20% of sequence in fragments shorter than    2014 bp
  25% of sequence in fragments shorter than    2356 bp
  30% of sequence in fragments shorter than    2698 bp
  35% of sequence in fragments shorter than    3045 bp
  40% of sequence in fragments shorter than    3408 bp
  45% of sequence in fragments shorter than    3777 bp
  50% of sequence in fragments shorter than    4171 bp
  55% of sequence in fragments shorter than    4589 bp
  60% of sequence in fragments shorter than    5044 bp
  65% of sequence in fragments shorter than    5555 bp
  70% of sequence in fragments shorter than    6127 bp
  75% of sequence in fragments shorter than    6793 bp
  80% of sequence in fragments shorter than    7610 bp
  85% of sequence in fragments shorter than    8663 bp
  90% of sequence in fragments shorter than   10224 bp
  95% of sequence in fragments shorter than   13502 bp

# HindIII.SapI (AAGCTT+GCTCTTC) double digest, 1237 thousand fragments (860365+377161 sites), 2408 bp average fragment length
   5% of sequence in fragments shorter than     835 bp
  10% of sequence in fragments shorter than    1278 bp
  15% of sequence in fragments shorter than    1646 bp
  20% of sequence in fragments shorter than    1998 bp
  25% of sequence in fragments shorter than    2354 bp
  30% of sequence in fragments shorter than    2700 bp
  35% of sequence in fragments shorter than    3049 bp
  40% of sequence in fragments shorter than    3416 bp
  45% of sequence in fragments shorter than    3797 bp
  50% of sequence in fragments shorter than    4198 bp
  55% of sequence in fragments shorter than    4632 bp
  60% of sequence in fragments shorter than    5096 bp
  65% of sequence in fragments shorter than    5616 bp
  70% of sequence in fragments shorter than    6210 bp
  75% of sequence in fragments shorter than    6898 bp
  80% of sequence in fragments shorter than    7745 bp
  85% of sequence in fragments shorter than    8831 bp
  90% of sequence in fragments shorter than   10394 bp
  95% of sequence in fragments shorter than   13432 bp

# HindIII.SmaI (AAGCTT+CCCGGG) double digest, 1236 thousand fragments (860365+376940 sites), 2409 bp average fragment length
   5% of sequence in fragments shorter than     834 bp
  10% of sequence in fragments shorter than    1274 bp
  15% of sequence in fragments shorter than    1658 bp
  20% of sequence in fragments shorter than    1983 bp
  25% of sequence in fragments shorter than    2337 bp
  30% of sequence in fragments shorter than    2684 bp
  35% of sequence in fragments shorter than    3035 bp
  40% of sequence in fragments shorter than    3400 bp
  45% of sequence in fragments shorter than    3782 bp
  50% of sequence in fragments shorter than    4182 bp
  55% of sequence in fragments shorter than    4614 bp
  60% of sequence in fragments shorter than    5084 bp
  65% of sequence in fragments shorter than    5601 bp
  70% of sequence in fragments shorter than    6195 bp
  75% of sequence in fragments shorter than    6885 bp
  80% of sequence in fragments shorter than    7712 bp
  85% of sequence in fragments shorter than    8776 bp
  90% of sequence in fragments shorter than   10305 bp
  95% of sequence in fragments shorter than   13167 bp

# EcoRI.HpaI (GAATTC+GTTAAC) double digest, 1236 thousand fragments (847401+389418 sites), 2410 bp average fragment length
   5% of sequence in fragments shorter than     808 bp
  10% of sequence in fragments shorter than    1266 bp
  15% of sequence in fragments shorter than    1660 bp
  20% of sequence in fragments shorter than    2034 bp
  25% of sequence in fragments shorter than    2395 bp
  30% of sequence in fragments shorter than    2749 bp
  35% of sequence in fragments shorter than    3110 bp
  40% of sequence in fragments shorter than    3482 bp
  45% of sequence in fragments shorter than    3869 bp
  50% of sequence in fragments shorter than    4280 bp
  55% of sequence in fragments shorter than    4727 bp
  60% of sequence in fragments shorter than    5211 bp
  65% of sequence in fragments shorter than    5750 bp
  70% of sequence in fragments shorter than    6370 bp
  75% of sequence in fragments shorter than    7094 bp
  80% of sequence in fragments shorter than    7976 bp
  85% of sequence in fragments shorter than    9107 bp
  90% of sequence in fragments shorter than   10773 bp
  95% of sequence in fragments shorter than   13946 bp

# AvaI.KasI (CYCGRG+GGCGCC) double digest, 1235 thousand fragments (1005714+230381 sites), 2411 bp average fragment length
   5% of sequence in fragments shorter than     801 bp
  10% of sequence in fragments shorter than    1354 bp
  15% of sequence in fragments shorter than    1891 bp
  20% of sequence in fragments shorter than    2436 bp
  25% of sequence in fragments shorter than    3013 bp
  30% of sequence in fragments shorter than    3633 bp
  35% of sequence in fragments shorter than    4297 bp
  40% of sequence in fragments shorter than    5018 bp
  45% of sequence in fragments shorter than    5798 bp
  50% of sequence in fragments shorter than    6668 bp
  55% of sequence in fragments shorter than    7632 bp
  60% of sequence in fragments shorter than    8727 bp
  65% of sequence in fragments shorter than    9983 bp
  70% of sequence in fragments shorter than   11436 bp
  75% of sequence in fragments shorter than   13213 bp
  80% of sequence in fragments shorter than   15438 bp
  85% of sequence in fragments shorter than   18386 bp
  90% of sequence in fragments shorter than   22830 bp
  95% of sequence in fragments shorter than   32128 bp

# AscI.BscGI (GGCGCGCC+CCCGT) double digest, 1235 thousand fragments (4751+1231070 sites), 2412 bp average fragment length
   5% of sequence in fragments shorter than     690 bp
  10% of sequence in fragments shorter than    1219 bp
  15% of sequence in fragments shorter than    1726 bp
  20% of sequence in fragments shorter than    2224 bp
  25% of sequence in fragments shorter than    2728 bp
  30% of sequence in fragments shorter than    3253 bp
  35% of sequence in fragments shorter than    3797 bp
  40% of sequence in fragments shorter than    4372 bp
  45% of sequence in fragments shorter than    4990 bp
  50% of sequence in fragments shorter than    5662 bp
  55% of sequence in fragments shorter than    6391 bp
  60% of sequence in fragments shorter than    7199 bp
  65% of sequence in fragments shorter than    8121 bp
  70% of sequence in fragments shorter than    9201 bp
  75% of sequence in fragments shorter than   10449 bp
  80% of sequence in fragments shorter than   11992 bp
  85% of sequence in fragments shorter than   13975 bp
  90% of sequence in fragments shorter than   16762 bp
  95% of sequence in fragments shorter than   21579 bp

# ApaI.BglII (GGGCCC+AGATCT) double digest, 1234 thousand fragments (460339+774732 sites), 2413 bp average fragment length
   5% of sequence in fragments shorter than     814 bp
  10% of sequence in fragments shorter than    1264 bp
  15% of sequence in fragments shorter than    1652 bp
  20% of sequence in fragments shorter than    2029 bp
  25% of sequence in fragments shorter than    2404 bp
  30% of sequence in fragments shorter than    2778 bp
  35% of sequence in fragments shorter than    3167 bp
  40% of sequence in fragments shorter than    3567 bp
  45% of sequence in fragments shorter than    3985 bp
  50% of sequence in fragments shorter than    4426 bp
  55% of sequence in fragments shorter than    4900 bp
  60% of sequence in fragments shorter than    5421 bp
  65% of sequence in fragments shorter than    5991 bp
  70% of sequence in fragments shorter than    6649 bp
  75% of sequence in fragments shorter than    7425 bp
  80% of sequence in fragments shorter than    8368 bp
  85% of sequence in fragments shorter than    9610 bp
  90% of sequence in fragments shorter than   11442 bp
  95% of sequence in fragments shorter than   15120 bp

# ApaLI.BclI (GTGCAC+TGATCA) double digest, 1234 thousand fragments (496255+738785 sites), 2413 bp average fragment length
   5% of sequence in fragments shorter than     862 bp
  10% of sequence in fragments shorter than    1292 bp
  15% of sequence in fragments shorter than    1662 bp
  20% of sequence in fragments shorter than    2005 bp
  25% of sequence in fragments shorter than    2338 bp
  30% of sequence in fragments shorter than    2646 bp
  35% of sequence in fragments shorter than    2986 bp
  40% of sequence in fragments shorter than    3337 bp
  45% of sequence in fragments shorter than    3699 bp
  50% of sequence in fragments shorter than    4087 bp
  55% of sequence in fragments shorter than    4503 bp
  60% of sequence in fragments shorter than    4952 bp
  65% of sequence in fragments shorter than    5452 bp
  70% of sequence in fragments shorter than    6022 bp
  75% of sequence in fragments shorter than    6686 bp
  80% of sequence in fragments shorter than    7481 bp
  85% of sequence in fragments shorter than    8521 bp
  90% of sequence in fragments shorter than   10014 bp
  95% of sequence in fragments shorter than   12982 bp

# HaeII.SacI (RGCGCY+GAGCTC) double digest, 1232 thousand fragments (630101+603323 sites), 2416 bp average fragment length
   5% of sequence in fragments shorter than     730 bp
  10% of sequence in fragments shorter than    1244 bp
  15% of sequence in fragments shorter than    1698 bp
  20% of sequence in fragments shorter than    2151 bp
  25% of sequence in fragments shorter than    2613 bp
  30% of sequence in fragments shorter than    3093 bp
  35% of sequence in fragments shorter than    3582 bp
  40% of sequence in fragments shorter than    4101 bp
  45% of sequence in fragments shorter than    4648 bp
  50% of sequence in fragments shorter than    5226 bp
  55% of sequence in fragments shorter than    5857 bp
  60% of sequence in fragments shorter than    6556 bp
  65% of sequence in fragments shorter than    7336 bp
  70% of sequence in fragments shorter than    8224 bp
  75% of sequence in fragments shorter than    9265 bp
  80% of sequence in fragments shorter than   10554 bp
  85% of sequence in fragments shorter than   12216 bp
  90% of sequence in fragments shorter than   14575 bp
  95% of sequence in fragments shorter than   18763 bp

# AccI.SnaBI (GTMKAC+TACGTA) double digest, 1232 thousand fragments (1101063+132299 sites), 2417 bp average fragment length
   5% of sequence in fragments shorter than     863 bp
  10% of sequence in fragments shorter than    1305 bp
  15% of sequence in fragments shorter than    1684 bp
  20% of sequence in fragments shorter than    2042 bp
  25% of sequence in fragments shorter than    2394 bp
  30% of sequence in fragments shorter than    2744 bp
  35% of sequence in fragments shorter than    3102 bp
  40% of sequence in fragments shorter than    3476 bp
  45% of sequence in fragments shorter than    3866 bp
  50% of sequence in fragments shorter than    4286 bp
  55% of sequence in fragments shorter than    4729 bp
  60% of sequence in fragments shorter than    5220 bp
  65% of sequence in fragments shorter than    5744 bp
  70% of sequence in fragments shorter than    6303 bp
  75% of sequence in fragments shorter than    7014 bp
  80% of sequence in fragments shorter than    7891 bp
  85% of sequence in fragments shorter than    9013 bp
  90% of sequence in fragments shorter than   10649 bp
  95% of sequence in fragments shorter than   13624 bp

# AvaI.SwaI (CYCGRG+ATTTAAAT) double digest, 1231 thousand fragments (1005714+226602 sites), 2418 bp average fragment length
   5% of sequence in fragments shorter than     806 bp
  10% of sequence in fragments shorter than    1295 bp
  15% of sequence in fragments shorter than    1744 bp
  20% of sequence in fragments shorter than    2182 bp
  25% of sequence in fragments shorter than    2624 bp
  30% of sequence in fragments shorter than    3078 bp
  35% of sequence in fragments shorter than    3557 bp
  40% of sequence in fragments shorter than    4064 bp
  45% of sequence in fragments shorter than    4601 bp
  50% of sequence in fragments shorter than    5176 bp
  55% of sequence in fragments shorter than    5803 bp
  60% of sequence in fragments shorter than    6508 bp
  65% of sequence in fragments shorter than    7299 bp
  70% of sequence in fragments shorter than    8204 bp
  75% of sequence in fragments shorter than    9290 bp
  80% of sequence in fragments shorter than   10608 bp
  85% of sequence in fragments shorter than   12370 bp
  90% of sequence in fragments shorter than   14993 bp
  95% of sequence in fragments shorter than   20375 bp

# AvaIII.SexAI (ATGCAT+ACCWGGT) double digest, 1230 thousand fragments (933357+297900 sites), 2421 bp average fragment length
   5% of sequence in fragments shorter than     857 bp
  10% of sequence in fragments shorter than    1297 bp
  15% of sequence in fragments shorter than    1679 bp
  20% of sequence in fragments shorter than    2040 bp
  25% of sequence in fragments shorter than    2383 bp
  30% of sequence in fragments shorter than    2733 bp
  35% of sequence in fragments shorter than    3092 bp
  40% of sequence in fragments shorter than    3463 bp
  45% of sequence in fragments shorter than    3856 bp
  50% of sequence in fragments shorter than    4273 bp
  55% of sequence in fragments shorter than    4727 bp
  60% of sequence in fragments shorter than    5220 bp
  65% of sequence in fragments shorter than    5777 bp
  70% of sequence in fragments shorter than    6403 bp
  75% of sequence in fragments shorter than    7132 bp
  80% of sequence in fragments shorter than    8014 bp
  85% of sequence in fragments shorter than    9170 bp
  90% of sequence in fragments shorter than   10848 bp
  95% of sequence in fragments shorter than   14048 bp

# ApaLI.FnuDII (GTGCAC+CGCG) double digest, 1229 thousand fragments (496255+733940 sites), 2423 bp average fragment length
   5% of sequence in fragments shorter than     942 bp
  10% of sequence in fragments shorter than    1577 bp
  15% of sequence in fragments shorter than    2140 bp
  20% of sequence in fragments shorter than    2682 bp
  25% of sequence in fragments shorter than    3226 bp
  30% of sequence in fragments shorter than    3781 bp
  35% of sequence in fragments shorter than    4362 bp
  40% of sequence in fragments shorter than    4973 bp
  45% of sequence in fragments shorter than    5611 bp
  50% of sequence in fragments shorter than    6252 bp
  55% of sequence in fragments shorter than    7012 bp
  60% of sequence in fragments shorter than    7868 bp
  65% of sequence in fragments shorter than    8803 bp
  70% of sequence in fragments shorter than    9882 bp
  75% of sequence in fragments shorter than   11152 bp
  80% of sequence in fragments shorter than   12733 bp
  85% of sequence in fragments shorter than   14786 bp
  90% of sequence in fragments shorter than   17802 bp
  95% of sequence in fragments shorter than   23535 bp

# SapI.XbaI (GCTCTTC+TCTAGA) double digest, 1227 thousand fragments (377161+850998 sites), 2427 bp average fragment length
   5% of sequence in fragments shorter than     851 bp
  10% of sequence in fragments shorter than    1259 bp
  15% of sequence in fragments shorter than    1628 bp
  20% of sequence in fragments shorter than    1977 bp
  25% of sequence in fragments shorter than    2318 bp
  30% of sequence in fragments shorter than    2676 bp
  35% of sequence in fragments shorter than    3038 bp
  40% of sequence in fragments shorter than    3408 bp
  45% of sequence in fragments shorter than    3794 bp
  50% of sequence in fragments shorter than    4211 bp
  55% of sequence in fragments shorter than    4650 bp
  60% of sequence in fragments shorter than    5132 bp
  65% of sequence in fragments shorter than    5666 bp
  70% of sequence in fragments shorter than    6269 bp
  75% of sequence in fragments shorter than    6970 bp
  80% of sequence in fragments shorter than    7811 bp
  85% of sequence in fragments shorter than    8896 bp
  90% of sequence in fragments shorter than   10455 bp
  95% of sequence in fragments shorter than   13243 bp

# SmaI.XbaI (CCCGGG+TCTAGA) double digest, 1227 thousand fragments (376940+850998 sites), 2427 bp average fragment length
   5% of sequence in fragments shorter than     846 bp
  10% of sequence in fragments shorter than    1272 bp
  15% of sequence in fragments shorter than    1641 bp
  20% of sequence in fragments shorter than    2001 bp
  25% of sequence in fragments shorter than    2343 bp
  30% of sequence in fragments shorter than    2707 bp
  35% of sequence in fragments shorter than    3070 bp
  40% of sequence in fragments shorter than    3444 bp
  45% of sequence in fragments shorter than    3834 bp
  50% of sequence in fragments shorter than    4247 bp
  55% of sequence in fragments shorter than    4690 bp
  60% of sequence in fragments shorter than    5165 bp
  65% of sequence in fragments shorter than    5693 bp
  70% of sequence in fragments shorter than    6290 bp
  75% of sequence in fragments shorter than    6982 bp
  80% of sequence in fragments shorter than    7813 bp
  85% of sequence in fragments shorter than    8863 bp
  90% of sequence in fragments shorter than   10357 bp
  95% of sequence in fragments shorter than   13001 bp

# AccI.NaeI (GTMKAC+GCCGGC) double digest, 1226 thousand fragments (1101063+125692 sites), 2430 bp average fragment length
   5% of sequence in fragments shorter than     862 bp
  10% of sequence in fragments shorter than    1308 bp
  15% of sequence in fragments shorter than    1689 bp
  20% of sequence in fragments shorter than    2045 bp
  25% of sequence in fragments shorter than    2397 bp
  30% of sequence in fragments shorter than    2745 bp
  35% of sequence in fragments shorter than    3096 bp
  40% of sequence in fragments shorter than    3464 bp
  45% of sequence in fragments shorter than    3846 bp
  50% of sequence in fragments shorter than    4255 bp
  55% of sequence in fragments shorter than    4686 bp
  60% of sequence in fragments shorter than    5159 bp
  65% of sequence in fragments shorter than    5658 bp
  70% of sequence in fragments shorter than    6187 bp
  75% of sequence in fragments shorter than    6869 bp
  80% of sequence in fragments shorter than    7675 bp
  85% of sequence in fragments shorter than    8717 bp
  90% of sequence in fragments shorter than   10223 bp
  95% of sequence in fragments shorter than   12865 bp

# BamHI.HindIII (GGATCC+AAGCTT) double digest, 1225 thousand fragments (365999+860365 sites), 2430 bp average fragment length
   5% of sequence in fragments shorter than     864 bp
  10% of sequence in fragments shorter than    1282 bp
  15% of sequence in fragments shorter than    1663 bp
  20% of sequence in fragments shorter than    1988 bp
  25% of sequence in fragments shorter than    2328 bp
  30% of sequence in fragments shorter than    2675 bp
  35% of sequence in fragments shorter than    3023 bp
  40% of sequence in fragments shorter than    3376 bp
  45% of sequence in fragments shorter than    3756 bp
  50% of sequence in fragments shorter than    4152 bp
  55% of sequence in fragments shorter than    4570 bp
  60% of sequence in fragments shorter than    5037 bp
  65% of sequence in fragments shorter than    5551 bp
  70% of sequence in fragments shorter than    6135 bp
  75% of sequence in fragments shorter than    6815 bp
  80% of sequence in fragments shorter than    7636 bp
  85% of sequence in fragments shorter than    8675 bp
  90% of sequence in fragments shorter than   10177 bp
  95% of sequence in fragments shorter than   12858 bp

# AvrII.HaeII (CCTAGG+RGCGCY) double digest, 1224 thousand fragments (594956+630101 sites), 2433 bp average fragment length
   5% of sequence in fragments shorter than     738 bp
  10% of sequence in fragments shorter than    1262 bp
  15% of sequence in fragments shorter than    1726 bp
  20% of sequence in fragments shorter than    2158 bp
  25% of sequence in fragments shorter than    2593 bp
  30% of sequence in fragments shorter than    3041 bp
  35% of sequence in fragments shorter than    3482 bp
  40% of sequence in fragments shorter than    3954 bp
  45% of sequence in fragments shorter than    4439 bp
  50% of sequence in fragments shorter than    4974 bp
  55% of sequence in fragments shorter than    5550 bp
  60% of sequence in fragments shorter than    6177 bp
  65% of sequence in fragments shorter than    6877 bp
  70% of sequence in fragments shorter than    7681 bp
  75% of sequence in fragments shorter than    8610 bp
  80% of sequence in fragments shorter than    9756 bp
  85% of sequence in fragments shorter than   11231 bp
  90% of sequence in fragments shorter than   13298 bp
  95% of sequence in fragments shorter than   16905 bp

# EcoRI.SapI (GAATTC+GCTCTTC) double digest, 1224 thousand fragments (847401+377161 sites), 2434 bp average fragment length
   5% of sequence in fragments shorter than     811 bp
  10% of sequence in fragments shorter than    1276 bp
  15% of sequence in fragments shorter than    1673 bp
  20% of sequence in fragments shorter than    2045 bp
  25% of sequence in fragments shorter than    2413 bp
  30% of sequence in fragments shorter than    2772 bp
  35% of sequence in fragments shorter than    3141 bp
  40% of sequence in fragments shorter than    3517 bp
  45% of sequence in fragments shorter than    3915 bp
  50% of sequence in fragments shorter than    4326 bp
  55% of sequence in fragments shorter than    4772 bp
  60% of sequence in fragments shorter than    5255 bp
  65% of sequence in fragments shorter than    5789 bp
  70% of sequence in fragments shorter than    6390 bp
  75% of sequence in fragments shorter than    7095 bp
  80% of sequence in fragments shorter than    7941 bp
  85% of sequence in fragments shorter than    9009 bp
  90% of sequence in fragments shorter than   10514 bp
  95% of sequence in fragments shorter than   13224 bp

# EcoRI.SmaI (GAATTC+CCCGGG) double digest, 1223 thousand fragments (847401+376940 sites), 2434 bp average fragment length
   5% of sequence in fragments shorter than     806 bp
  10% of sequence in fragments shorter than    1285 bp
  15% of sequence in fragments shorter than    1689 bp
  20% of sequence in fragments shorter than    2072 bp
  25% of sequence in fragments shorter than    2443 bp
  30% of sequence in fragments shorter than    2810 bp
  35% of sequence in fragments shorter than    3186 bp
  40% of sequence in fragments shorter than    3560 bp
  45% of sequence in fragments shorter than    3963 bp
  50% of sequence in fragments shorter than    4385 bp
  55% of sequence in fragments shorter than    4848 bp
  60% of sequence in fragments shorter than    5345 bp
  65% of sequence in fragments shorter than    5892 bp
  70% of sequence in fragments shorter than    6511 bp
  75% of sequence in fragments shorter than    7231 bp
  80% of sequence in fragments shorter than    8104 bp
  85% of sequence in fragments shorter than    9213 bp
  90% of sequence in fragments shorter than   10752 bp
  95% of sequence in fragments shorter than   13542 bp

# SexAI.StuI (ACCWGGT+AGGCCT) double digest, 1223 thousand fragments (297900+925729 sites), 2436 bp average fragment length
   5% of sequence in fragments shorter than     729 bp
  10% of sequence in fragments shorter than    1241 bp
  15% of sequence in fragments shorter than    1681 bp
  20% of sequence in fragments shorter than    2093 bp
  25% of sequence in fragments shorter than    2536 bp
  30% of sequence in fragments shorter than    2976 bp
  35% of sequence in fragments shorter than    3428 bp
  40% of sequence in fragments shorter than    3892 bp
  45% of sequence in fragments shorter than    4386 bp
  50% of sequence in fragments shorter than    4917 bp
  55% of sequence in fragments shorter than    5485 bp
  60% of sequence in fragments shorter than    6098 bp
  65% of sequence in fragments shorter than    6788 bp
  70% of sequence in fragments shorter than    7559 bp
  75% of sequence in fragments shorter than    8446 bp
  80% of sequence in fragments shorter than    9533 bp
  85% of sequence in fragments shorter than   10908 bp
  90% of sequence in fragments shorter than   12826 bp
  95% of sequence in fragments shorter than   16077 bp

# AccI.XhoI (GTMKAC+CTCGAG) double digest, 1221 thousand fragments (1101063+121308 sites), 2438 bp average fragment length
   5% of sequence in fragments shorter than     867 bp
  10% of sequence in fragments shorter than    1300 bp
  15% of sequence in fragments shorter than    1674 bp
  20% of sequence in fragments shorter than    2027 bp
  25% of sequence in fragments shorter than    2377 bp
  30% of sequence in fragments shorter than    2723 bp
  35% of sequence in fragments shorter than    3073 bp
  40% of sequence in fragments shorter than    3439 bp
  45% of sequence in fragments shorter than    3814 bp
  50% of sequence in fragments shorter than    4215 bp
  55% of sequence in fragments shorter than    4606 bp
  60% of sequence in fragments shorter than    5063 bp
  65% of sequence in fragments shorter than    5572 bp
  70% of sequence in fragments shorter than    6139 bp
  75% of sequence in fragments shorter than    6823 bp
  80% of sequence in fragments shorter than    7631 bp
  85% of sequence in fragments shorter than    8665 bp
  90% of sequence in fragments shorter than   10172 bp
  95% of sequence in fragments shorter than   12820 bp

# AvaIII.KpnI (ATGCAT+GGTACC) double digest, 1220 thousand fragments (933357+287974 sites), 2440 bp average fragment length
   5% of sequence in fragments shorter than     882 bp
  10% of sequence in fragments shorter than    1303 bp
  15% of sequence in fragments shorter than    1675 bp
  20% of sequence in fragments shorter than    2028 bp
  25% of sequence in fragments shorter than    2369 bp
  30% of sequence in fragments shorter than    2719 bp
  35% of sequence in fragments shorter than    3073 bp
  40% of sequence in fragments shorter than    3440 bp
  45% of sequence in fragments shorter than    3825 bp
  50% of sequence in fragments shorter than    4238 bp
  55% of sequence in fragments shorter than    4685 bp
  60% of sequence in fragments shorter than    5165 bp
  65% of sequence in fragments shorter than    5708 bp
  70% of sequence in fragments shorter than    6327 bp
  75% of sequence in fragments shorter than    7071 bp
  80% of sequence in fragments shorter than    7975 bp
  85% of sequence in fragments shorter than    9154 bp
  90% of sequence in fragments shorter than   10924 bp
  95% of sequence in fragments shorter than   14375 bp

# BglII.EcoRV (AGATCT+GATATC) double digest, 1220 thousand fragments (774732+446473 sites), 2441 bp average fragment length
   5% of sequence in fragments shorter than     840 bp
  10% of sequence in fragments shorter than    1275 bp
  15% of sequence in fragments shorter than    1650 bp
  20% of sequence in fragments shorter than    2004 bp
  25% of sequence in fragments shorter than    2359 bp
  30% of sequence in fragments shorter than    2714 bp
  35% of sequence in fragments shorter than    3077 bp
  40% of sequence in fragments shorter than    3449 bp
  45% of sequence in fragments shorter than    3842 bp
  50% of sequence in fragments shorter than    4261 bp
  55% of sequence in fragments shorter than    4708 bp
  60% of sequence in fragments shorter than    5195 bp
  65% of sequence in fragments shorter than    5746 bp
  70% of sequence in fragments shorter than    6376 bp
  75% of sequence in fragments shorter than    7110 bp
  80% of sequence in fragments shorter than    8024 bp
  85% of sequence in fragments shorter than    9202 bp
  90% of sequence in fragments shorter than   10947 bp
  95% of sequence in fragments shorter than   14384 bp

# AvaIII.NheI (ATGCAT+GCTAGC) double digest, 1220 thousand fragments (933357+287384 sites), 2442 bp average fragment length
   5% of sequence in fragments shorter than     882 bp
  10% of sequence in fragments shorter than    1329 bp
  15% of sequence in fragments shorter than    1711 bp
  20% of sequence in fragments shorter than    2041 bp
  25% of sequence in fragments shorter than    2385 bp
  30% of sequence in fragments shorter than    2727 bp
  35% of sequence in fragments shorter than    3072 bp
  40% of sequence in fragments shorter than    3445 bp
  45% of sequence in fragments shorter than    3841 bp
  50% of sequence in fragments shorter than    4265 bp
  55% of sequence in fragments shorter than    4719 bp
  60% of sequence in fragments shorter than    5215 bp
  65% of sequence in fragments shorter than    5764 bp
  70% of sequence in fragments shorter than    6395 bp
  75% of sequence in fragments shorter than    7150 bp
  80% of sequence in fragments shorter than    8066 bp
  85% of sequence in fragments shorter than    9274 bp
  90% of sequence in fragments shorter than   11095 bp
  95% of sequence in fragments shorter than   14678 bp

# ApaI.NcoI (GGGCCC+CCATGG) double digest, 1219 thousand fragments (460339+759594 sites), 2443 bp average fragment length
   5% of sequence in fragments shorter than     805 bp
  10% of sequence in fragments shorter than    1297 bp
  15% of sequence in fragments shorter than    1739 bp
  20% of sequence in fragments shorter than    2174 bp
  25% of sequence in fragments shorter than    2609 bp
  30% of sequence in fragments shorter than    3054 bp
  35% of sequence in fragments shorter than    3517 bp
  40% of sequence in fragments shorter than    4001 bp
  45% of sequence in fragments shorter than    4510 bp
  50% of sequence in fragments shorter than    5033 bp
  55% of sequence in fragments shorter than    5609 bp
  60% of sequence in fragments shorter than    6229 bp
  65% of sequence in fragments shorter than    6951 bp
  70% of sequence in fragments shorter than    7780 bp
  75% of sequence in fragments shorter than    8773 bp
  80% of sequence in fragments shorter than    9982 bp
  85% of sequence in fragments shorter than   11559 bp
  90% of sequence in fragments shorter than   13893 bp
  95% of sequence in fragments shorter than   18613 bp

# PpuMI.SnaBI (RGGWCCY+TACGTA) double digest, 1217 thousand fragments (1085138+132299 sites), 2448 bp average fragment length
   5% of sequence in fragments shorter than     812 bp
  10% of sequence in fragments shorter than    1298 bp
  15% of sequence in fragments shorter than    1728 bp
  20% of sequence in fragments shorter than    2146 bp
  25% of sequence in fragments shorter than    2568 bp
  30% of sequence in fragments shorter than    2993 bp
  35% of sequence in fragments shorter than    3422 bp
  40% of sequence in fragments shorter than    3838 bp
  45% of sequence in fragments shorter than    4322 bp
  50% of sequence in fragments shorter than    4843 bp
  55% of sequence in fragments shorter than    5408 bp
  60% of sequence in fragments shorter than    6035 bp
  65% of sequence in fragments shorter than    6737 bp
  70% of sequence in fragments shorter than    7537 bp
  75% of sequence in fragments shorter than    8468 bp
  80% of sequence in fragments shorter than    9630 bp
  85% of sequence in fragments shorter than   11122 bp
  90% of sequence in fragments shorter than   13379 bp
  95% of sequence in fragments shorter than   17779 bp

# BamHI.XbaI (GGATCC+TCTAGA) double digest, 1216 thousand fragments (365999+850998 sites), 2449 bp average fragment length
   5% of sequence in fragments shorter than     835 bp
  10% of sequence in fragments shorter than    1274 bp
  15% of sequence in fragments shorter than    1657 bp
  20% of sequence in fragments shorter than    2022 bp
  25% of sequence in fragments shorter than    2373 bp
  30% of sequence in fragments shorter than    2737 bp
  35% of sequence in fragments shorter than    3100 bp
  40% of sequence in fragments shorter than    3475 bp
  45% of sequence in fragments shorter than    3864 bp
  50% of sequence in fragments shorter than    4277 bp
  55% of sequence in fragments shorter than    4710 bp
  60% of sequence in fragments shorter than    5188 bp
  65% of sequence in fragments shorter than    5712 bp
  70% of sequence in fragments shorter than    6304 bp
  75% of sequence in fragments shorter than    6983 bp
  80% of sequence in fragments shorter than    7818 bp
  85% of sequence in fragments shorter than    8870 bp
  90% of sequence in fragments shorter than   10335 bp
  95% of sequence in fragments shorter than   12944 bp

# PvuII.SnaBI (CAGCTG+TACGTA) double digest, 1216 thousand fragments (1084260+132299 sites), 2450 bp average fragment length
   5% of sequence in fragments shorter than     841 bp
  10% of sequence in fragments shorter than    1297 bp
  15% of sequence in fragments shorter than    1695 bp
  20% of sequence in fragments shorter than    2087 bp
  25% of sequence in fragments shorter than    2475 bp
  30% of sequence in fragments shorter than    2852 bp
  35% of sequence in fragments shorter than    3250 bp
  40% of sequence in fragments shorter than    3667 bp
  45% of sequence in fragments shorter than    4114 bp
  50% of sequence in fragments shorter than    4593 bp
  55% of sequence in fragments shorter than    5112 bp
  60% of sequence in fragments shorter than    5677 bp
  65% of sequence in fragments shorter than    6303 bp
  70% of sequence in fragments shorter than    7008 bp
  75% of sequence in fragments shorter than    7849 bp
  80% of sequence in fragments shorter than    8860 bp
  85% of sequence in fragments shorter than   10209 bp
  90% of sequence in fragments shorter than   12155 bp
  95% of sequence in fragments shorter than   15910 bp

# AflII.HgaI (CTTAAG+GACGC) double digest, 1214 thousand fragments (643284+571889 sites), 2453 bp average fragment length
   5% of sequence in fragments shorter than     854 bp
  10% of sequence in fragments shorter than    1322 bp
  15% of sequence in fragments shorter than    1735 bp
  20% of sequence in fragments shorter than    2122 bp
  25% of sequence in fragments shorter than    2501 bp
  30% of sequence in fragments shorter than    2875 bp
  35% of sequence in fragments shorter than    3257 bp
  40% of sequence in fragments shorter than    3651 bp
  45% of sequence in fragments shorter than    4062 bp
  50% of sequence in fragments shorter than    4497 bp
  55% of sequence in fragments shorter than    4968 bp
  60% of sequence in fragments shorter than    5493 bp
  65% of sequence in fragments shorter than    6063 bp
  70% of sequence in fragments shorter than    6716 bp
  75% of sequence in fragments shorter than    7469 bp
  80% of sequence in fragments shorter than    8392 bp
  85% of sequence in fragments shorter than    9599 bp
  90% of sequence in fragments shorter than   11304 bp
  95% of sequence in fragments shorter than   14528 bp

# AflII.EciI (CTTAAG+GGCGGA) double digest, 1214 thousand fragments (643284+571273 sites), 2454 bp average fragment length
   5% of sequence in fragments shorter than     839 bp
  10% of sequence in fragments shorter than    1299 bp
  15% of sequence in fragments shorter than    1703 bp
  20% of sequence in fragments shorter than    2083 bp
  25% of sequence in fragments shorter than    2450 bp
  30% of sequence in fragments shorter than    2824 bp
  35% of sequence in fragments shorter than    3204 bp
  40% of sequence in fragments shorter than    3603 bp
  45% of sequence in fragments shorter than    4015 bp
  50% of sequence in fragments shorter than    4457 bp
  55% of sequence in fragments shorter than    4937 bp
  60% of sequence in fragments shorter than    5461 bp
  65% of sequence in fragments shorter than    6047 bp
  70% of sequence in fragments shorter than    6722 bp
  75% of sequence in fragments shorter than    7515 bp
  80% of sequence in fragments shorter than    8484 bp
  85% of sequence in fragments shorter than    9746 bp
  90% of sequence in fragments shorter than   11635 bp
  95% of sequence in fragments shorter than   15413 bp

# BspHI.BsrBI (TCATGA+CCGCTC) double digest, 1213 thousand fragments (978290+235523 sites), 2455 bp average fragment length
   5% of sequence in fragments shorter than     873 bp
  10% of sequence in fragments shorter than    1330 bp
  15% of sequence in fragments shorter than    1715 bp
  20% of sequence in fragments shorter than    2067 bp
  25% of sequence in fragments shorter than    2408 bp
  30% of sequence in fragments shorter than    2759 bp
  35% of sequence in fragments shorter than    3108 bp
  40% of sequence in fragments shorter than    3476 bp
  45% of sequence in fragments shorter than    3860 bp
  50% of sequence in fragments shorter than    4262 bp
  55% of sequence in fragments shorter than    4693 bp
  60% of sequence in fragments shorter than    5162 bp
  65% of sequence in fragments shorter than    5676 bp
  70% of sequence in fragments shorter than    6255 bp
  75% of sequence in fragments shorter than    6938 bp
  80% of sequence in fragments shorter than    7760 bp
  85% of sequence in fragments shorter than    8811 bp
  90% of sequence in fragments shorter than   10334 bp
  95% of sequence in fragments shorter than   13115 bp

# KpnI.StuI (GGTACC+AGGCCT) double digest, 1213 thousand fragments (287974+925729 sites), 2456 bp average fragment length
   5% of sequence in fragments shorter than     742 bp
  10% of sequence in fragments shorter than    1245 bp
  15% of sequence in fragments shorter than    1664 bp
  20% of sequence in fragments shorter than    2112 bp
  25% of sequence in fragments shorter than    2564 bp
  30% of sequence in fragments shorter than    3009 bp
  35% of sequence in fragments shorter than    3469 bp
  40% of sequence in fragments shorter than    3941 bp
  45% of sequence in fragments shorter than    4437 bp
  50% of sequence in fragments shorter than    4970 bp
  55% of sequence in fragments shorter than    5543 bp
  60% of sequence in fragments shorter than    6157 bp
  65% of sequence in fragments shorter than    6849 bp
  70% of sequence in fragments shorter than    7633 bp
  75% of sequence in fragments shorter than    8527 bp
  80% of sequence in fragments shorter than    9613 bp
  85% of sequence in fragments shorter than   11004 bp
  90% of sequence in fragments shorter than   12912 bp
  95% of sequence in fragments shorter than   16208 bp

# BamHI.EcoRI (GGATCC+GAATTC) double digest, 1212 thousand fragments (365999+847401 sites), 2456 bp average fragment length
   5% of sequence in fragments shorter than     837 bp
  10% of sequence in fragments shorter than    1318 bp
  15% of sequence in fragments shorter than    1690 bp
  20% of sequence in fragments shorter than    2068 bp
  25% of sequence in fragments shorter than    2424 bp
  30% of sequence in fragments shorter than    2784 bp
  35% of sequence in fragments shorter than    3151 bp
  40% of sequence in fragments shorter than    3517 bp
  45% of sequence in fragments shorter than    3911 bp
  50% of sequence in fragments shorter than    4321 bp
  55% of sequence in fragments shorter than    4760 bp
  60% of sequence in fragments shorter than    5233 bp
  65% of sequence in fragments shorter than    5762 bp
  70% of sequence in fragments shorter than    6356 bp
  75% of sequence in fragments shorter than    7045 bp
  80% of sequence in fragments shorter than    7872 bp
  85% of sequence in fragments shorter than    8917 bp
  90% of sequence in fragments shorter than   10407 bp
  95% of sequence in fragments shorter than   12997 bp

# NheI.StuI (GCTAGC+AGGCCT) double digest, 1212 thousand fragments (287384+925729 sites), 2457 bp average fragment length
   5% of sequence in fragments shorter than     751 bp
  10% of sequence in fragments shorter than    1227 bp
  15% of sequence in fragments shorter than    1678 bp
  20% of sequence in fragments shorter than    2110 bp
  25% of sequence in fragments shorter than    2560 bp
  30% of sequence in fragments shorter than    3001 bp
  35% of sequence in fragments shorter than    3453 bp
  40% of sequence in fragments shorter than    3922 bp
  45% of sequence in fragments shorter than    4413 bp
  50% of sequence in fragments shorter than    4940 bp
  55% of sequence in fragments shorter than    5499 bp
  60% of sequence in fragments shorter than    6112 bp
  65% of sequence in fragments shorter than    6798 bp
  70% of sequence in fragments shorter than    7566 bp
  75% of sequence in fragments shorter than    8452 bp
  80% of sequence in fragments shorter than    9522 bp
  85% of sequence in fragments shorter than   10893 bp
  90% of sequence in fragments shorter than   12773 bp
  95% of sequence in fragments shorter than   15990 bp

# NaeI.PpuMI (GCCGGC+RGGWCCY) double digest, 1210 thousand fragments (125692+1085138 sites), 2461 bp average fragment length
   5% of sequence in fragments shorter than     788 bp
  10% of sequence in fragments shorter than    1307 bp
  15% of sequence in fragments shorter than    1791 bp
  20% of sequence in fragments shorter than    2256 bp
  25% of sequence in fragments shorter than    2729 bp
  30% of sequence in fragments shorter than    3209 bp
  35% of sequence in fragments shorter than    3671 bp
  40% of sequence in fragments shorter than    4182 bp
  45% of sequence in fragments shorter than    4747 bp
  50% of sequence in fragments shorter than    5358 bp
  55% of sequence in fragments shorter than    6027 bp
  60% of sequence in fragments shorter than    6766 bp
  65% of sequence in fragments shorter than    7603 bp
  70% of sequence in fragments shorter than    8557 bp
  75% of sequence in fragments shorter than    9695 bp
  80% of sequence in fragments shorter than   11073 bp
  85% of sequence in fragments shorter than   12927 bp
  90% of sequence in fragments shorter than   15707 bp
  95% of sequence in fragments shorter than   21455 bp

# NaeI.PvuII (GCCGGC+CAGCTG) double digest, 1209 thousand fragments (125692+1084260 sites), 2463 bp average fragment length
   5% of sequence in fragments shorter than     820 bp
  10% of sequence in fragments shorter than    1299 bp
  15% of sequence in fragments shorter than    1734 bp
  20% of sequence in fragments shorter than    2164 bp
  25% of sequence in fragments shorter than    2589 bp
  30% of sequence in fragments shorter than    3008 bp
  35% of sequence in fragments shorter than    3461 bp
  40% of sequence in fragments shorter than    3936 bp
  45% of sequence in fragments shorter than    4447 bp
  50% of sequence in fragments shorter than    4997 bp
  55% of sequence in fragments shorter than    5594 bp
  60% of sequence in fragments shorter than    6245 bp
  65% of sequence in fragments shorter than    6967 bp
  70% of sequence in fragments shorter than    7792 bp
  75% of sequence in fragments shorter than    8766 bp
  80% of sequence in fragments shorter than   10000 bp
  85% of sequence in fragments shorter than   11611 bp
  90% of sequence in fragments shorter than   13990 bp
  95% of sequence in fragments shorter than   18697 bp

# ApaLI.BsaAI (GTGCAC+YACGTR) double digest, 1209 thousand fragments (496255+713801 sites), 2463 bp average fragment length
   5% of sequence in fragments shorter than     890 bp
  10% of sequence in fragments shorter than    1346 bp
  15% of sequence in fragments shorter than    1749 bp
  20% of sequence in fragments shorter than    2124 bp
  25% of sequence in fragments shorter than    2505 bp
  30% of sequence in fragments shorter than    2879 bp
  35% of sequence in fragments shorter than    3256 bp
  40% of sequence in fragments shorter than    3634 bp
  45% of sequence in fragments shorter than    4044 bp
  50% of sequence in fragments shorter than    4471 bp
  55% of sequence in fragments shorter than    4942 bp
  60% of sequence in fragments shorter than    5459 bp
  65% of sequence in fragments shorter than    6029 bp
  70% of sequence in fragments shorter than    6681 bp
  75% of sequence in fragments shorter than    7438 bp
  80% of sequence in fragments shorter than    8346 bp
  85% of sequence in fragments shorter than    9506 bp
  90% of sequence in fragments shorter than   11173 bp
  95% of sequence in fragments shorter than   14145 bp

# BspHI.KasI (TCATGA+GGCGCC) double digest, 1208 thousand fragments (978290+230381 sites), 2466 bp average fragment length
   5% of sequence in fragments shorter than     875 bp
  10% of sequence in fragments shorter than    1335 bp
  15% of sequence in fragments shorter than    1732 bp
  20% of sequence in fragments shorter than    2085 bp
  25% of sequence in fragments shorter than    2426 bp
  30% of sequence in fragments shorter than    2777 bp
  35% of sequence in fragments shorter than    3131 bp
  40% of sequence in fragments shorter than    3499 bp
  45% of sequence in fragments shorter than    3869 bp
  50% of sequence in fragments shorter than    4263 bp
  55% of sequence in fragments shorter than    4697 bp
  60% of sequence in fragments shorter than    5172 bp
  65% of sequence in fragments shorter than    5690 bp
  70% of sequence in fragments shorter than    6280 bp
  75% of sequence in fragments shorter than    6959 bp
  80% of sequence in fragments shorter than    7803 bp
  85% of sequence in fragments shorter than    8881 bp
  90% of sequence in fragments shorter than   10444 bp
  95% of sequence in fragments shorter than   13511 bp

# AflII.MfeI (CTTAAG+CAATTG) double digest, 1207 thousand fragments (643284+564303 sites), 2468 bp average fragment length
   5% of sequence in fragments shorter than     857 bp
  10% of sequence in fragments shorter than    1302 bp
  15% of sequence in fragments shorter than    1697 bp
  20% of sequence in fragments shorter than    2054 bp
  25% of sequence in fragments shorter than    2402 bp
  30% of sequence in fragments shorter than    2757 bp
  35% of sequence in fragments shorter than    3119 bp
  40% of sequence in fragments shorter than    3497 bp
  45% of sequence in fragments shorter than    3887 bp
  50% of sequence in fragments shorter than    4308 bp
  55% of sequence in fragments shorter than    4754 bp
  60% of sequence in fragments shorter than    5247 bp
  65% of sequence in fragments shorter than    5804 bp
  70% of sequence in fragments shorter than    6446 bp
  75% of sequence in fragments shorter than    7204 bp
  80% of sequence in fragments shorter than    8152 bp
  85% of sequence in fragments shorter than    9429 bp
  90% of sequence in fragments shorter than   11356 bp
  95% of sequence in fragments shorter than   15433 bp

# PpuMI.XhoI (RGGWCCY+CTCGAG) double digest, 1206 thousand fragments (1085138+121308 sites), 2470 bp average fragment length
   5% of sequence in fragments shorter than     793 bp
  10% of sequence in fragments shorter than    1292 bp
  15% of sequence in fragments shorter than    1753 bp
  20% of sequence in fragments shorter than    2194 bp
  25% of sequence in fragments shorter than    2642 bp
  30% of sequence in fragments shorter than    3072 bp
  35% of sequence in fragments shorter than    3539 bp
  40% of sequence in fragments shorter than    4025 bp
  45% of sequence in fragments shorter than    4553 bp
  50% of sequence in fragments shorter than    5129 bp
  55% of sequence in fragments shorter than    5759 bp
  60% of sequence in fragments shorter than    6449 bp
  65% of sequence in fragments shorter than    7231 bp
  70% of sequence in fragments shorter than    8130 bp
  75% of sequence in fragments shorter than    9187 bp
  80% of sequence in fragments shorter than   10520 bp
  85% of sequence in fragments shorter than   12262 bp
  90% of sequence in fragments shorter than   14832 bp
  95% of sequence in fragments shorter than   20154 bp

# EcoRV.NcoI (GATATC+CCATGG) double digest, 1205 thousand fragments (446473+759594 sites), 2471 bp average fragment length
   5% of sequence in fragments shorter than     863 bp
  10% of sequence in fragments shorter than    1305 bp
  15% of sequence in fragments shorter than    1694 bp
  20% of sequence in fragments shorter than    2057 bp
  25% of sequence in fragments shorter than    2411 bp
  30% of sequence in fragments shorter than    2738 bp
  35% of sequence in fragments shorter than    3098 bp
  40% of sequence in fragments shorter than    3469 bp
  45% of sequence in fragments shorter than    3864 bp
  50% of sequence in fragments shorter than    4277 bp
  55% of sequence in fragments shorter than    4718 bp
  60% of sequence in fragments shorter than    5203 bp
  65% of sequence in fragments shorter than    5735 bp
  70% of sequence in fragments shorter than    6348 bp
  75% of sequence in fragments shorter than    7062 bp
  80% of sequence in fragments shorter than    7929 bp
  85% of sequence in fragments shorter than    9055 bp
  90% of sequence in fragments shorter than   10677 bp
  95% of sequence in fragments shorter than   13736 bp

# PvuII.XhoI (CAGCTG+CTCGAG) double digest, 1205 thousand fragments (1084260+121308 sites), 2472 bp average fragment length
   5% of sequence in fragments shorter than     820 bp
  10% of sequence in fragments shorter than    1291 bp
  15% of sequence in fragments shorter than    1712 bp
  20% of sequence in fragments shorter than    2120 bp
  25% of sequence in fragments shorter than    2527 bp
  30% of sequence in fragments shorter than    2932 bp
  35% of sequence in fragments shorter than    3362 bp
  40% of sequence in fragments shorter than    3814 bp
  45% of sequence in fragments shorter than    4297 bp
  50% of sequence in fragments shorter than    4817 bp
  55% of sequence in fragments shorter than    5382 bp
  60% of sequence in fragments shorter than    6003 bp
  65% of sequence in fragments shorter than    6691 bp
  70% of sequence in fragments shorter than    7469 bp
  75% of sequence in fragments shorter than    8407 bp
  80% of sequence in fragments shorter than    9568 bp
  85% of sequence in fragments shorter than   11111 bp
  90% of sequence in fragments shorter than   13349 bp
  95% of sequence in fragments shorter than   17784 bp

# BspHI.SwaI (TCATGA+ATTTAAAT) double digest, 1204 thousand fragments (978290+226602 sites), 2474 bp average fragment length
   5% of sequence in fragments shorter than     863 bp
  10% of sequence in fragments shorter than    1312 bp
  15% of sequence in fragments shorter than    1705 bp
  20% of sequence in fragments shorter than    2059 bp
  25% of sequence in fragments shorter than    2400 bp
  30% of sequence in fragments shorter than    2756 bp
  35% of sequence in fragments shorter than    3116 bp
  40% of sequence in fragments shorter than    3495 bp
  45% of sequence in fragments shorter than    3896 bp
  50% of sequence in fragments shorter than    4318 bp
  55% of sequence in fragments shorter than    4773 bp
  60% of sequence in fragments shorter than    5271 bp
  65% of sequence in fragments shorter than    5822 bp
  70% of sequence in fragments shorter than    6467 bp
  75% of sequence in fragments shorter than    7223 bp
  80% of sequence in fragments shorter than    8171 bp
  85% of sequence in fragments shorter than    9434 bp
  90% of sequence in fragments shorter than   11363 bp
  95% of sequence in fragments shorter than   15536 bp

# HaeII.HgaI (RGCGCY+GACGC) double digest, 1201 thousand fragments (630101+571889 sites), 2480 bp average fragment length
   5% of sequence in fragments shorter than     739 bp
  10% of sequence in fragments shorter than    1348 bp
  15% of sequence in fragments shorter than    1927 bp
  20% of sequence in fragments shorter than    2498 bp
  25% of sequence in fragments shorter than    3097 bp
  30% of sequence in fragments shorter than    3716 bp
  35% of sequence in fragments shorter than    4370 bp
  40% of sequence in fragments shorter than    5064 bp
  45% of sequence in fragments shorter than    5806 bp
  50% of sequence in fragments shorter than    6613 bp
  55% of sequence in fragments shorter than    7483 bp
  60% of sequence in fragments shorter than    8463 bp
  65% of sequence in fragments shorter than    9591 bp
  70% of sequence in fragments shorter than   10868 bp
  75% of sequence in fragments shorter than   12383 bp
  80% of sequence in fragments shorter than   14232 bp
  85% of sequence in fragments shorter than   16630 bp
  90% of sequence in fragments shorter than   20072 bp
  95% of sequence in fragments shorter than   25985 bp

# ApaLI.BceAI (GTGCAC+ACGGC) double digest, 1200 thousand fragments (496255+705178 sites), 2481 bp average fragment length
   5% of sequence in fragments shorter than     832 bp
  10% of sequence in fragments shorter than    1322 bp
  15% of sequence in fragments shorter than    1765 bp
  20% of sequence in fragments shorter than    2190 bp
  25% of sequence in fragments shorter than    2618 bp
  30% of sequence in fragments shorter than    3051 bp
  35% of sequence in fragments shorter than    3496 bp
  40% of sequence in fragments shorter than    3969 bp
  45% of sequence in fragments shorter than    4463 bp
  50% of sequence in fragments shorter than    4983 bp
  55% of sequence in fragments shorter than    5517 bp
  60% of sequence in fragments shorter than    6114 bp
  65% of sequence in fragments shorter than    6824 bp
  70% of sequence in fragments shorter than    7636 bp
  75% of sequence in fragments shorter than    8585 bp
  80% of sequence in fragments shorter than    9742 bp
  85% of sequence in fragments shorter than   11258 bp
  90% of sequence in fragments shorter than   13433 bp
  95% of sequence in fragments shorter than   17494 bp

# EciI.HaeII (GGCGGA+RGCGCY) double digest, 1200 thousand fragments (571273+630101 sites), 2481 bp average fragment length
   5% of sequence in fragments shorter than     751 bp
  10% of sequence in fragments shorter than    1358 bp
  15% of sequence in fragments shorter than    1932 bp
  20% of sequence in fragments shorter than    2507 bp
  25% of sequence in fragments shorter than    3128 bp
  30% of sequence in fragments shorter than    3779 bp
  35% of sequence in fragments shorter than    4488 bp
  40% of sequence in fragments shorter than    5257 bp
  45% of sequence in fragments shorter than    6092 bp
  50% of sequence in fragments shorter than    7005 bp
  55% of sequence in fragments shorter than    8002 bp
  60% of sequence in fragments shorter than    9151 bp
  65% of sequence in fragments shorter than   10467 bp
  70% of sequence in fragments shorter than   11998 bp
  75% of sequence in fragments shorter than   13804 bp
  80% of sequence in fragments shorter than   16032 bp
  85% of sequence in fragments shorter than   18925 bp
  90% of sequence in fragments shorter than   23055 bp
  95% of sequence in fragments shorter than   30357 bp

# NdeI.SexAI (CATATG+ACCWGGT) double digest, 1200 thousand fragments (903360+297900 sites), 2481 bp average fragment length
   5% of sequence in fragments shorter than     847 bp
  10% of sequence in fragments shorter than    1280 bp
  15% of sequence in fragments shorter than    1683 bp
  20% of sequence in fragments shorter than    2062 bp
  25% of sequence in fragments shorter than    2434 bp
  30% of sequence in fragments shorter than    2805 bp
  35% of sequence in fragments shorter than    3180 bp
  40% of sequence in fragments shorter than    3572 bp
  45% of sequence in fragments shorter than    3976 bp
  50% of sequence in fragments shorter than    4408 bp
  55% of sequence in fragments shorter than    4858 bp
  60% of sequence in fragments shorter than    5355 bp
  65% of sequence in fragments shorter than    5912 bp
  70% of sequence in fragments shorter than    6544 bp
  75% of sequence in fragments shorter than    7300 bp
  80% of sequence in fragments shorter than    8213 bp
  85% of sequence in fragments shorter than    9438 bp
  90% of sequence in fragments shorter than   11207 bp
  95% of sequence in fragments shorter than   14869 bp

# ApaI.BclI (GGGCCC+TGATCA) double digest, 1198 thousand fragments (460339+738785 sites), 2485 bp average fragment length
   5% of sequence in fragments shorter than     862 bp
  10% of sequence in fragments shorter than    1317 bp
  15% of sequence in fragments shorter than    1715 bp
  20% of sequence in fragments shorter than    2098 bp
  25% of sequence in fragments shorter than    2471 bp
  30% of sequence in fragments shorter than    2839 bp
  35% of sequence in fragments shorter than    3223 bp
  40% of sequence in fragments shorter than    3622 bp
  45% of sequence in fragments shorter than    4035 bp
  50% of sequence in fragments shorter than    4479 bp
  55% of sequence in fragments shorter than    4951 bp
  60% of sequence in fragments shorter than    5470 bp
  65% of sequence in fragments shorter than    6045 bp
  70% of sequence in fragments shorter than    6711 bp
  75% of sequence in fragments shorter than    7475 bp
  80% of sequence in fragments shorter than    8422 bp
  85% of sequence in fragments shorter than    9649 bp
  90% of sequence in fragments shorter than   11484 bp
  95% of sequence in fragments shorter than   15156 bp

# AvrII.SacI (CCTAGG+GAGCTC) double digest, 1197 thousand fragments (594956+603323 sites), 2487 bp average fragment length
   5% of sequence in fragments shorter than     848 bp
  10% of sequence in fragments shorter than    1312 bp
  15% of sequence in fragments shorter than    1713 bp
  20% of sequence in fragments shorter than    2087 bp
  25% of sequence in fragments shorter than    2453 bp
  30% of sequence in fragments shorter than    2788 bp
  35% of sequence in fragments shorter than    3162 bp
  40% of sequence in fragments shorter than    3552 bp
  45% of sequence in fragments shorter than    3968 bp
  50% of sequence in fragments shorter than    4409 bp
  55% of sequence in fragments shorter than    4883 bp
  60% of sequence in fragments shorter than    5409 bp
  65% of sequence in fragments shorter than    5997 bp
  70% of sequence in fragments shorter than    6668 bp
  75% of sequence in fragments shorter than    7452 bp
  80% of sequence in fragments shorter than    8413 bp
  85% of sequence in fragments shorter than    9679 bp
  90% of sequence in fragments shorter than   11521 bp
  95% of sequence in fragments shorter than   15217 bp

# BstEII.HindIII (GGTNACC+AAGCTT) double digest, 1195 thousand fragments (335865+860365 sites), 2492 bp average fragment length
   5% of sequence in fragments shorter than     877 bp
  10% of sequence in fragments shorter than    1334 bp
  15% of sequence in fragments shorter than    1730 bp
  20% of sequence in fragments shorter than    2063 bp
  25% of sequence in fragments shorter than    2425 bp
  30% of sequence in fragments shorter than    2782 bp
  35% of sequence in fragments shorter than    3142 bp
  40% of sequence in fragments shorter than    3515 bp
  45% of sequence in fragments shorter than    3902 bp
  50% of sequence in fragments shorter than    4312 bp
  55% of sequence in fragments shorter than    4754 bp
  60% of sequence in fragments shorter than    5234 bp
  65% of sequence in fragments shorter than    5764 bp
  70% of sequence in fragments shorter than    6370 bp
  75% of sequence in fragments shorter than    7067 bp
  80% of sequence in fragments shorter than    7926 bp
  85% of sequence in fragments shorter than    9022 bp
  90% of sequence in fragments shorter than   10597 bp
  95% of sequence in fragments shorter than   13548 bp

# HaeII.MfeI (RGCGCY+CAATTG) double digest, 1193 thousand fragments (630101+564303 sites), 2495 bp average fragment length
   5% of sequence in fragments shorter than     816 bp
  10% of sequence in fragments shorter than    1341 bp
  15% of sequence in fragments shorter than    1803 bp
  20% of sequence in fragments shorter than    2218 bp
  25% of sequence in fragments shorter than    2627 bp
  30% of sequence in fragments shorter than    3045 bp
  35% of sequence in fragments shorter than    3457 bp
  40% of sequence in fragments shorter than    3887 bp
  45% of sequence in fragments shorter than    4329 bp
  50% of sequence in fragments shorter than    4800 bp
  55% of sequence in fragments shorter than    5308 bp
  60% of sequence in fragments shorter than    5855 bp
  65% of sequence in fragments shorter than    6467 bp
  70% of sequence in fragments shorter than    7143 bp
  75% of sequence in fragments shorter than    7939 bp
  80% of sequence in fragments shorter than    8894 bp
  85% of sequence in fragments shorter than   10101 bp
  90% of sequence in fragments shorter than   11739 bp
  95% of sequence in fragments shorter than   14526 bp

# ApaI.FnuDII (GGGCCC+CGCG) double digest, 1193 thousand fragments (460339+733940 sites), 2496 bp average fragment length
   5% of sequence in fragments shorter than     911 bp
  10% of sequence in fragments shorter than    1641 bp
  15% of sequence in fragments shorter than    2341 bp
  20% of sequence in fragments shorter than    3051 bp
  25% of sequence in fragments shorter than    3793 bp
  30% of sequence in fragments shorter than    4569 bp
  35% of sequence in fragments shorter than    5398 bp
  40% of sequence in fragments shorter than    6301 bp
  45% of sequence in fragments shorter than    7259 bp
  50% of sequence in fragments shorter than    8319 bp
  55% of sequence in fragments shorter than    9474 bp
  60% of sequence in fragments shorter than   10771 bp
  65% of sequence in fragments shorter than   12234 bp
  70% of sequence in fragments shorter than   13945 bp
  75% of sequence in fragments shorter than   16046 bp
  80% of sequence in fragments shorter than   18606 bp
  85% of sequence in fragments shorter than   22061 bp
  90% of sequence in fragments shorter than   27100 bp
  95% of sequence in fragments shorter than   37269 bp

# AflII.SphI (CTTAAG+GCATGC) double digest, 1193 thousand fragments (643284+550952 sites), 2496 bp average fragment length
   5% of sequence in fragments shorter than     880 bp
  10% of sequence in fragments shorter than    1329 bp
  15% of sequence in fragments shorter than    1714 bp
  20% of sequence in fragments shorter than    2071 bp
  25% of sequence in fragments shorter than    2416 bp
  30% of sequence in fragments shorter than    2762 bp
  35% of sequence in fragments shorter than    3107 bp
  40% of sequence in fragments shorter than    3472 bp
  45% of sequence in fragments shorter than    3839 bp
  50% of sequence in fragments shorter than    4246 bp
  55% of sequence in fragments shorter than    4675 bp
  60% of sequence in fragments shorter than    5141 bp
  65% of sequence in fragments shorter than    5662 bp
  70% of sequence in fragments shorter than    6244 bp
  75% of sequence in fragments shorter than    6928 bp
  80% of sequence in fragments shorter than    7761 bp
  85% of sequence in fragments shorter than    8855 bp
  90% of sequence in fragments shorter than   10436 bp
  95% of sequence in fragments shorter than   13531 bp

# ApaLI.BciVI (GTGCAC+GTATCC) double digest, 1191 thousand fragments (496255+696093 sites), 2500 bp average fragment length
   5% of sequence in fragments shorter than     900 bp
  10% of sequence in fragments shorter than    1351 bp
  15% of sequence in fragments shorter than    1717 bp
  20% of sequence in fragments shorter than    2049 bp
  25% of sequence in fragments shorter than    2394 bp
  30% of sequence in fragments shorter than    2747 bp
  35% of sequence in fragments shorter than    3105 bp
  40% of sequence in fragments shorter than    3474 bp
  45% of sequence in fragments shorter than    3860 bp
  50% of sequence in fragments shorter than    4261 bp
  55% of sequence in fragments shorter than    4691 bp
  60% of sequence in fragments shorter than    5163 bp
  65% of sequence in fragments shorter than    5685 bp
  70% of sequence in fragments shorter than    6270 bp
  75% of sequence in fragments shorter than    6958 bp
  80% of sequence in fragments shorter than    7780 bp
  85% of sequence in fragments shorter than    8848 bp
  90% of sequence in fragments shorter than   10348 bp
  95% of sequence in fragments shorter than   13127 bp

# KpnI.NdeI (GGTACC+CATATG) double digest, 1190 thousand fragments (287974+903360 sites), 2502 bp average fragment length
   5% of sequence in fragments shorter than     867 bp
  10% of sequence in fragments shorter than    1294 bp
  15% of sequence in fragments shorter than    1699 bp
  20% of sequence in fragments shorter than    2086 bp
  25% of sequence in fragments shorter than    2475 bp
  30% of sequence in fragments shorter than    2859 bp
  35% of sequence in fragments shorter than    3254 bp
  40% of sequence in fragments shorter than    3656 bp
  45% of sequence in fragments shorter than    4087 bp
  50% of sequence in fragments shorter than    4546 bp
  55% of sequence in fragments shorter than    5039 bp
  60% of sequence in fragments shorter than    5580 bp
  65% of sequence in fragments shorter than    6190 bp
  70% of sequence in fragments shorter than    6884 bp
  75% of sequence in fragments shorter than    7713 bp
  80% of sequence in fragments shorter than    8740 bp
  85% of sequence in fragments shorter than   10105 bp
  90% of sequence in fragments shorter than   12144 bp
  95% of sequence in fragments shorter than   16334 bp

# NdeI.NheI (CATATG+GCTAGC) double digest, 1190 thousand fragments (903360+287384 sites), 2503 bp average fragment length
   5% of sequence in fragments shorter than     864 bp
  10% of sequence in fragments shorter than    1315 bp
  15% of sequence in fragments shorter than    1719 bp
  20% of sequence in fragments shorter than    2105 bp
  25% of sequence in fragments shorter than    2504 bp
  30% of sequence in fragments shorter than    2869 bp
  35% of sequence in fragments shorter than    3268 bp
  40% of sequence in fragments shorter than    3687 bp
  45% of sequence in fragments shorter than    4128 bp
  50% of sequence in fragments shorter than    4592 bp
  55% of sequence in fragments shorter than    5099 bp
  60% of sequence in fragments shorter than    5652 bp
  65% of sequence in fragments shorter than    6275 bp
  70% of sequence in fragments shorter than    7001 bp
  75% of sequence in fragments shorter than    7863 bp
  80% of sequence in fragments shorter than    8924 bp
  85% of sequence in fragments shorter than   10322 bp
  90% of sequence in fragments shorter than   12465 bp
  95% of sequence in fragments shorter than   16939 bp

# AccI.ClaI (GTMKAC+ATCGAT) double digest, 1187 thousand fragments (1101063+86477 sites), 2510 bp average fragment length
   5% of sequence in fragments shorter than     889 bp
  10% of sequence in fragments shorter than    1341 bp
  15% of sequence in fragments shorter than    1727 bp
  20% of sequence in fragments shorter than    2090 bp
  25% of sequence in fragments shorter than    2441 bp
  30% of sequence in fragments shorter than    2791 bp
  35% of sequence in fragments shorter than    3159 bp
  40% of sequence in fragments shorter than    3530 bp
  45% of sequence in fragments shorter than    3926 bp
  50% of sequence in fragments shorter than    4348 bp
  55% of sequence in fragments shorter than    4802 bp
  60% of sequence in fragments shorter than    5295 bp
  65% of sequence in fragments shorter than    5838 bp
  70% of sequence in fragments shorter than    6417 bp
  75% of sequence in fragments shorter than    7141 bp
  80% of sequence in fragments shorter than    8023 bp
  85% of sequence in fragments shorter than    9172 bp
  90% of sequence in fragments shorter than   10822 bp
  95% of sequence in fragments shorter than   13850 bp

# AflII.ScaI (CTTAAG+AGTACT) double digest, 1186 thousand fragments (643284+543793 sites), 2511 bp average fragment length
   5% of sequence in fragments shorter than     876 bp
  10% of sequence in fragments shorter than    1328 bp
  15% of sequence in fragments shorter than    1716 bp
  20% of sequence in fragments shorter than    2079 bp
  25% of sequence in fragments shorter than    2434 bp
  30% of sequence in fragments shorter than    2790 bp
  35% of sequence in fragments shorter than    3158 bp
  40% of sequence in fragments shorter than    3542 bp
  45% of sequence in fragments shorter than    3936 bp
  50% of sequence in fragments shorter than    4366 bp
  55% of sequence in fragments shorter than    4819 bp
  60% of sequence in fragments shorter than    5323 bp
  65% of sequence in fragments shorter than    5887 bp
  70% of sequence in fragments shorter than    6547 bp
  75% of sequence in fragments shorter than    7328 bp
  80% of sequence in fragments shorter than    8303 bp
  85% of sequence in fragments shorter than    9583 bp
  90% of sequence in fragments shorter than   11550 bp
  95% of sequence in fragments shorter than   16087 bp

# BstEII.XbaI (GGTNACC+TCTAGA) double digest, 1186 thousand fragments (335865+850998 sites), 2511 bp average fragment length
   5% of sequence in fragments shorter than     864 bp
  10% of sequence in fragments shorter than    1334 bp
  15% of sequence in fragments shorter than    1679 bp
  20% of sequence in fragments shorter than    2046 bp
  25% of sequence in fragments shorter than    2397 bp
  30% of sequence in fragments shorter than    2757 bp
  35% of sequence in fragments shorter than    3123 bp
  40% of sequence in fragments shorter than    3497 bp
  45% of sequence in fragments shorter than    3893 bp
  50% of sequence in fragments shorter than    4311 bp
  55% of sequence in fragments shorter than    4763 bp
  60% of sequence in fragments shorter than    5250 bp
  65% of sequence in fragments shorter than    5792 bp
  70% of sequence in fragments shorter than    6399 bp
  75% of sequence in fragments shorter than    7101 bp
  80% of sequence in fragments shorter than    7956 bp
  85% of sequence in fragments shorter than    9026 bp
  90% of sequence in fragments shorter than   10557 bp
  95% of sequence in fragments shorter than   13302 bp

# BclI.EcoRV (TGATCA+GATATC) double digest, 1184 thousand fragments (738785+446473 sites), 2515 bp average fragment length
   5% of sequence in fragments shorter than     864 bp
  10% of sequence in fragments shorter than    1321 bp
  15% of sequence in fragments shorter than    1709 bp
  20% of sequence in fragments shorter than    2077 bp
  25% of sequence in fragments shorter than    2433 bp
  30% of sequence in fragments shorter than    2791 bp
  35% of sequence in fragments shorter than    3166 bp
  40% of sequence in fragments shorter than    3548 bp
  45% of sequence in fragments shorter than    3949 bp
  50% of sequence in fragments shorter than    4367 bp
  55% of sequence in fragments shorter than    4824 bp
  60% of sequence in fragments shorter than    5323 bp
  65% of sequence in fragments shorter than    5878 bp
  70% of sequence in fragments shorter than    6519 bp
  75% of sequence in fragments shorter than    7254 bp
  80% of sequence in fragments shorter than    8178 bp
  85% of sequence in fragments shorter than    9354 bp
  90% of sequence in fragments shorter than   11151 bp
  95% of sequence in fragments shorter than   14594 bp

# BstEII.EcoRI (GGTNACC+GAATTC) double digest, 1182 thousand fragments (335865+847401 sites), 2519 bp average fragment length
   5% of sequence in fragments shorter than     853 bp
  10% of sequence in fragments shorter than    1346 bp
  15% of sequence in fragments shorter than    1749 bp
  20% of sequence in fragments shorter than    2114 bp
  25% of sequence in fragments shorter than    2491 bp
  30% of sequence in fragments shorter than    2857 bp
  35% of sequence in fragments shorter than    3233 bp
  40% of sequence in fragments shorter than    3618 bp
  45% of sequence in fragments shorter than    4023 bp
  50% of sequence in fragments shorter than    4451 bp
  55% of sequence in fragments shorter than    4901 bp
  60% of sequence in fragments shorter than    5393 bp
  65% of sequence in fragments shorter than    5935 bp
  70% of sequence in fragments shorter than    6545 bp
  75% of sequence in fragments shorter than    7261 bp
  80% of sequence in fragments shorter than    8119 bp
  85% of sequence in fragments shorter than    9202 bp
  90% of sequence in fragments shorter than   10735 bp
  95% of sequence in fragments shorter than   13476 bp

# HaeII.SphI (RGCGCY+GCATGC) double digest, 1180 thousand fragments (630101+550952 sites), 2524 bp average fragment length
   5% of sequence in fragments shorter than     789 bp
  10% of sequence in fragments shorter than    1310 bp
  15% of sequence in fragments shorter than    1779 bp
  20% of sequence in fragments shorter than    2226 bp
  25% of sequence in fragments shorter than    2685 bp
  30% of sequence in fragments shorter than    3158 bp
  35% of sequence in fragments shorter than    3640 bp
  40% of sequence in fragments shorter than    4154 bp
  45% of sequence in fragments shorter than    4693 bp
  50% of sequence in fragments shorter than    5278 bp
  55% of sequence in fragments shorter than    5904 bp
  60% of sequence in fragments shorter than    6598 bp
  65% of sequence in fragments shorter than    7358 bp
  70% of sequence in fragments shorter than    8242 bp
  75% of sequence in fragments shorter than    9293 bp
  80% of sequence in fragments shorter than   10547 bp
  85% of sequence in fragments shorter than   12187 bp
  90% of sequence in fragments shorter than   14467 bp
  95% of sequence in fragments shorter than   18379 bp

# EcoRV.FnuDII (GATATC+CGCG) double digest, 1179 thousand fragments (446473+733940 sites), 2525 bp average fragment length
   5% of sequence in fragments shorter than    1019 bp
  10% of sequence in fragments shorter than    1679 bp
  15% of sequence in fragments shorter than    2237 bp
  20% of sequence in fragments shorter than    2761 bp
  25% of sequence in fragments shorter than    3267 bp
  30% of sequence in fragments shorter than    3787 bp
  35% of sequence in fragments shorter than    4319 bp
  40% of sequence in fragments shorter than    4865 bp
  45% of sequence in fragments shorter than    5441 bp
  50% of sequence in fragments shorter than    6047 bp
  55% of sequence in fragments shorter than    6699 bp
  60% of sequence in fragments shorter than    7415 bp
  65% of sequence in fragments shorter than    8206 bp
  70% of sequence in fragments shorter than    9093 bp
  75% of sequence in fragments shorter than   10138 bp
  80% of sequence in fragments shorter than   11432 bp
  85% of sequence in fragments shorter than   13081 bp
  90% of sequence in fragments shorter than   15424 bp
  95% of sequence in fragments shorter than   19599 bp

# AclI.AvaI (AACGTT+CYCGRG) double digest, 1177 thousand fragments (172706+1005714 sites), 2529 bp average fragment length
   5% of sequence in fragments shorter than     831 bp
  10% of sequence in fragments shorter than    1339 bp
  15% of sequence in fragments shorter than    1824 bp
  20% of sequence in fragments shorter than    2296 bp
  25% of sequence in fragments shorter than    2788 bp
  30% of sequence in fragments shorter than    3297 bp
  35% of sequence in fragments shorter than    3813 bp
  40% of sequence in fragments shorter than    4383 bp
  45% of sequence in fragments shorter than    4998 bp
  50% of sequence in fragments shorter than    5665 bp
  55% of sequence in fragments shorter than    6404 bp
  60% of sequence in fragments shorter than    7226 bp
  65% of sequence in fragments shorter than    8175 bp
  70% of sequence in fragments shorter than    9269 bp
  75% of sequence in fragments shorter than   10534 bp
  80% of sequence in fragments shorter than   12121 bp
  85% of sequence in fragments shorter than   14199 bp
  90% of sequence in fragments shorter than   17263 bp
  95% of sequence in fragments shorter than   23178 bp

# HgaI.SacI (GACGC+GAGCTC) double digest, 1174 thousand fragments (571889+603323 sites), 2536 bp average fragment length
   5% of sequence in fragments shorter than     842 bp
  10% of sequence in fragments shorter than    1340 bp
  15% of sequence in fragments shorter than    1795 bp
  20% of sequence in fragments shorter than    2233 bp
  25% of sequence in fragments shorter than    2683 bp
  30% of sequence in fragments shorter than    3142 bp
  35% of sequence in fragments shorter than    3620 bp
  40% of sequence in fragments shorter than    4128 bp
  45% of sequence in fragments shorter than    4655 bp
  50% of sequence in fragments shorter than    5225 bp
  55% of sequence in fragments shorter than    5839 bp
  60% of sequence in fragments shorter than    6524 bp
  65% of sequence in fragments shorter than    7270 bp
  70% of sequence in fragments shorter than    8156 bp
  75% of sequence in fragments shorter than    9194 bp
  80% of sequence in fragments shorter than   10455 bp
  85% of sequence in fragments shorter than   12110 bp
  90% of sequence in fragments shorter than   14539 bp
  95% of sequence in fragments shorter than   19029 bp

# BglII.SpeI (AGATCT+ACTAGT) double digest, 1174 thousand fragments (774732+400286 sites), 2537 bp average fragment length
   5% of sequence in fragments shorter than     888 bp
  10% of sequence in fragments shorter than    1334 bp
  15% of sequence in fragments shorter than    1704 bp
  20% of sequence in fragments shorter than    2064 bp
  25% of sequence in fragments shorter than    2418 bp
  30% of sequence in fragments shorter than    2759 bp
  35% of sequence in fragments shorter than    3125 bp
  40% of sequence in fragments shorter than    3476 bp
  45% of sequence in fragments shorter than    3871 bp
  50% of sequence in fragments shorter than    4293 bp
  55% of sequence in fragments shorter than    4747 bp
  60% of sequence in fragments shorter than    5237 bp
  65% of sequence in fragments shorter than    5792 bp
  70% of sequence in fragments shorter than    6432 bp
  75% of sequence in fragments shorter than    7183 bp
  80% of sequence in fragments shorter than    8124 bp
  85% of sequence in fragments shorter than    9349 bp
  90% of sequence in fragments shorter than   11181 bp
  95% of sequence in fragments shorter than   14959 bp

# EciI.SacI (GGCGGA+GAGCTC) double digest, 1174 thousand fragments (571273+603323 sites), 2537 bp average fragment length
   5% of sequence in fragments shorter than     838 bp
  10% of sequence in fragments shorter than    1319 bp
  15% of sequence in fragments shorter than    1752 bp
  20% of sequence in fragments shorter than    2172 bp
  25% of sequence in fragments shorter than    2595 bp
  30% of sequence in fragments shorter than    3033 bp
  35% of sequence in fragments shorter than    3491 bp
  40% of sequence in fragments shorter than    3981 bp
  45% of sequence in fragments shorter than    4500 bp
  50% of sequence in fragments shorter than    5061 bp
  55% of sequence in fragments shorter than    5678 bp
  60% of sequence in fragments shorter than    6359 bp
  65% of sequence in fragments shorter than    7131 bp
  70% of sequence in fragments shorter than    8022 bp
  75% of sequence in fragments shorter than    9097 bp
  80% of sequence in fragments shorter than   10401 bp
  85% of sequence in fragments shorter than   12156 bp
  90% of sequence in fragments shorter than   14738 bp
  95% of sequence in fragments shorter than   19746 bp

# ApaI.BsaAI (GGGCCC+YACGTR) double digest, 1173 thousand fragments (460339+713801 sites), 2538 bp average fragment length
   5% of sequence in fragments shorter than     861 bp
  10% of sequence in fragments shorter than    1348 bp
  15% of sequence in fragments shorter than    1792 bp
  20% of sequence in fragments shorter than    2220 bp
  25% of sequence in fragments shorter than    2653 bp
  30% of sequence in fragments shorter than    3087 bp
  35% of sequence in fragments shorter than    3516 bp
  40% of sequence in fragments shorter than    3987 bp
  45% of sequence in fragments shorter than    4473 bp
  50% of sequence in fragments shorter than    4998 bp
  55% of sequence in fragments shorter than    5569 bp
  60% of sequence in fragments shorter than    6188 bp
  65% of sequence in fragments shorter than    6890 bp
  70% of sequence in fragments shorter than    7685 bp
  75% of sequence in fragments shorter than    8604 bp
  80% of sequence in fragments shorter than    9723 bp
  85% of sequence in fragments shorter than   11166 bp
  90% of sequence in fragments shorter than   13278 bp
  95% of sequence in fragments shorter than   17129 bp

# HaeII.ScaI (RGCGCY+AGTACT) double digest, 1173 thousand fragments (630101+543793 sites), 2539 bp average fragment length
   5% of sequence in fragments shorter than     824 bp
  10% of sequence in fragments shorter than    1347 bp
  15% of sequence in fragments shorter than    1805 bp
  20% of sequence in fragments shorter than    2235 bp
  25% of sequence in fragments shorter than    2665 bp
  30% of sequence in fragments shorter than    3102 bp
  35% of sequence in fragments shorter than    3546 bp
  40% of sequence in fragments shorter than    4004 bp
  45% of sequence in fragments shorter than    4483 bp
  50% of sequence in fragments shorter than    4994 bp
  55% of sequence in fragments shorter than    5536 bp
  60% of sequence in fragments shorter than    6125 bp
  65% of sequence in fragments shorter than    6777 bp
  70% of sequence in fragments shorter than    7514 bp
  75% of sequence in fragments shorter than    8375 bp
  80% of sequence in fragments shorter than    9404 bp
  85% of sequence in fragments shorter than   10711 bp
  90% of sequence in fragments shorter than   12566 bp
  95% of sequence in fragments shorter than   15764 bp

# AatII.AccI (GACGTC+GTMKAC) double digest, 1171 thousand fragments (71089+1101063 sites), 2543 bp average fragment length
   5% of sequence in fragments shorter than     904 bp
  10% of sequence in fragments shorter than    1363 bp
  15% of sequence in fragments shorter than    1755 bp
  20% of sequence in fragments shorter than    2125 bp
  25% of sequence in fragments shorter than    2490 bp
  30% of sequence in fragments shorter than    2846 bp
  35% of sequence in fragments shorter than    3220 bp
  40% of sequence in fragments shorter than    3598 bp
  45% of sequence in fragments shorter than    3997 bp
  50% of sequence in fragments shorter than    4422 bp
  55% of sequence in fragments shorter than    4872 bp
  60% of sequence in fragments shorter than    5366 bp
  65% of sequence in fragments shorter than    5897 bp
  70% of sequence in fragments shorter than    6460 bp
  75% of sequence in fragments shorter than    7175 bp
  80% of sequence in fragments shorter than    8045 bp
  85% of sequence in fragments shorter than    9159 bp
  90% of sequence in fragments shorter than   10759 bp
  95% of sequence in fragments shorter than   13620 bp

# ClaI.PpuMI (ATCGAT+RGGWCCY) double digest, 1171 thousand fragments (86477+1085138 sites), 2544 bp average fragment length
   5% of sequence in fragments shorter than     828 bp
  10% of sequence in fragments shorter than    1334 bp
  15% of sequence in fragments shorter than    1797 bp
  20% of sequence in fragments shorter than    2239 bp
  25% of sequence in fragments shorter than    2682 bp
  30% of sequence in fragments shorter than    3132 bp
  35% of sequence in fragments shorter than    3574 bp
  40% of sequence in fragments shorter than    4057 bp
  45% of sequence in fragments shorter than    4581 bp
  50% of sequence in fragments shorter than    5148 bp
  55% of sequence in fragments shorter than    5768 bp
  60% of sequence in fragments shorter than    6451 bp
  65% of sequence in fragments shorter than    7221 bp
  70% of sequence in fragments shorter than    8102 bp
  75% of sequence in fragments shorter than    9136 bp
  80% of sequence in fragments shorter than   10415 bp
  85% of sequence in fragments shorter than   12117 bp
  90% of sequence in fragments shorter than   14653 bp
  95% of sequence in fragments shorter than   19763 bp

# ClaI.PvuII (ATCGAT+CAGCTG) double digest, 1170 thousand fragments (86477+1084260 sites), 2546 bp average fragment length
   5% of sequence in fragments shorter than     865 bp
  10% of sequence in fragments shorter than    1340 bp
  15% of sequence in fragments shorter than    1763 bp
  20% of sequence in fragments shorter than    2177 bp
  25% of sequence in fragments shorter than    2584 bp
  30% of sequence in fragments shorter than    2976 bp
  35% of sequence in fragments shorter than    3403 bp
  40% of sequence in fragments shorter than    3849 bp
  45% of sequence in fragments shorter than    4323 bp
  50% of sequence in fragments shorter than    4831 bp
  55% of sequence in fragments shorter than    5388 bp
  60% of sequence in fragments shorter than    5991 bp
  65% of sequence in fragments shorter than    6662 bp
  70% of sequence in fragments shorter than    7413 bp
  75% of sequence in fragments shorter than    8324 bp
  80% of sequence in fragments shorter than    9428 bp
  85% of sequence in fragments shorter than   10890 bp
  90% of sequence in fragments shorter than   13038 bp
  95% of sequence in fragments shorter than   17282 bp

# AvaIII.BsrBI (ATGCAT+CCGCTC) double digest, 1168 thousand fragments (933357+235523 sites), 2550 bp average fragment length
   5% of sequence in fragments shorter than     910 bp
  10% of sequence in fragments shorter than    1389 bp
  15% of sequence in fragments shorter than    1806 bp
  20% of sequence in fragments shorter than    2183 bp
  25% of sequence in fragments shorter than    2561 bp
  30% of sequence in fragments shorter than    2935 bp
  35% of sequence in fragments shorter than    3314 bp
  40% of sequence in fragments shorter than    3711 bp
  45% of sequence in fragments shorter than    4127 bp
  50% of sequence in fragments shorter than    4568 bp
  55% of sequence in fragments shorter than    5041 bp
  60% of sequence in fragments shorter than    5557 bp
  65% of sequence in fragments shorter than    6129 bp
  70% of sequence in fragments shorter than    6782 bp
  75% of sequence in fragments shorter than    7536 bp
  80% of sequence in fragments shorter than    8449 bp
  85% of sequence in fragments shorter than    9628 bp
  90% of sequence in fragments shorter than   11341 bp
  95% of sequence in fragments shorter than   14430 bp

# AccI.SacII (GTMKAC+CCGCGG) double digest, 1167 thousand fragments (1101063+66901 sites), 2552 bp average fragment length
   5% of sequence in fragments shorter than     906 bp
  10% of sequence in fragments shorter than    1376 bp
  15% of sequence in fragments shorter than    1779 bp
  20% of sequence in fragments shorter than    2159 bp
  25% of sequence in fragments shorter than    2530 bp
  30% of sequence in fragments shorter than    2895 bp
  35% of sequence in fragments shorter than    3276 bp
  40% of sequence in fragments shorter than    3665 bp
  45% of sequence in fragments shorter than    4075 bp
  50% of sequence in fragments shorter than    4507 bp
  55% of sequence in fragments shorter than    4970 bp
  60% of sequence in fragments shorter than    5471 bp
  65% of sequence in fragments shorter than    6000 bp
  70% of sequence in fragments shorter than    6584 bp
  75% of sequence in fragments shorter than    7313 bp
  80% of sequence in fragments shorter than    8192 bp
  85% of sequence in fragments shorter than    9326 bp
  90% of sequence in fragments shorter than   10951 bp
  95% of sequence in fragments shorter than   13874 bp

# MfeI.SacI (CAATTG+GAGCTC) double digest, 1167 thousand fragments (564303+603323 sites), 2553 bp average fragment length
   5% of sequence in fragments shorter than     896 bp
  10% of sequence in fragments shorter than    1354 bp
  15% of sequence in fragments shorter than    1752 bp
  20% of sequence in fragments shorter than    2109 bp
  25% of sequence in fragments shorter than    2449 bp
  30% of sequence in fragments shorter than    2788 bp
  35% of sequence in fragments shorter than    3153 bp
  40% of sequence in fragments shorter than    3520 bp
  45% of sequence in fragments shorter than    3909 bp
  50% of sequence in fragments shorter than    4319 bp
  55% of sequence in fragments shorter than    4757 bp
  60% of sequence in fragments shorter than    5238 bp
  65% of sequence in fragments shorter than    5774 bp
  70% of sequence in fragments shorter than    6375 bp
  75% of sequence in fragments shorter than    7073 bp
  80% of sequence in fragments shorter than    7915 bp
  85% of sequence in fragments shorter than    8995 bp
  90% of sequence in fragments shorter than   10549 bp
  95% of sequence in fragments shorter than   13355 bp

# AvrII.HgaI (CCTAGG+GACGC) double digest, 1166 thousand fragments (594956+571889 sites), 2554 bp average fragment length
   5% of sequence in fragments shorter than     844 bp
  10% of sequence in fragments shorter than    1355 bp
  15% of sequence in fragments shorter than    1806 bp
  20% of sequence in fragments shorter than    2242 bp
  25% of sequence in fragments shorter than    2675 bp
  30% of sequence in fragments shorter than    3101 bp
  35% of sequence in fragments shorter than    3543 bp
  40% of sequence in fragments shorter than    4004 bp
  45% of sequence in fragments shorter than    4477 bp
  50% of sequence in fragments shorter than    4999 bp
  55% of sequence in fragments shorter than    5562 bp
  60% of sequence in fragments shorter than    6186 bp
  65% of sequence in fragments shorter than    6889 bp
  70% of sequence in fragments shorter than    7688 bp
  75% of sequence in fragments shorter than    8618 bp
  80% of sequence in fragments shorter than    9752 bp
  85% of sequence in fragments shorter than   11274 bp
  90% of sequence in fragments shorter than   13471 bp
  95% of sequence in fragments shorter than   17709 bp

# AvrII.EciI (CCTAGG+GGCGGA) double digest, 1165 thousand fragments (594956+571273 sites), 2556 bp average fragment length
   5% of sequence in fragments shorter than     835 bp
  10% of sequence in fragments shorter than    1336 bp
  15% of sequence in fragments shorter than    1779 bp
  20% of sequence in fragments shorter than    2205 bp
  25% of sequence in fragments shorter than    2625 bp
  30% of sequence in fragments shorter than    3050 bp
  35% of sequence in fragments shorter than    3500 bp
  40% of sequence in fragments shorter than    3964 bp
  45% of sequence in fragments shorter than    4446 bp
  50% of sequence in fragments shorter than    4977 bp
  55% of sequence in fragments shorter than    5548 bp
  60% of sequence in fragments shorter than    6188 bp
  65% of sequence in fragments shorter than    6903 bp
  70% of sequence in fragments shorter than    7727 bp
  75% of sequence in fragments shorter than    8687 bp
  80% of sequence in fragments shorter than    9897 bp
  85% of sequence in fragments shorter than   11493 bp
  90% of sequence in fragments shorter than   13889 bp
  95% of sequence in fragments shorter than   18692 bp

# ApaI.BceAI (GGGCCC+ACGGC) double digest, 1165 thousand fragments (460339+705178 sites), 2557 bp average fragment length
   5% of sequence in fragments shorter than     800 bp
  10% of sequence in fragments shorter than    1367 bp
  15% of sequence in fragments shorter than    1908 bp
  20% of sequence in fragments shorter than    2449 bp
  25% of sequence in fragments shorter than    3000 bp
  30% of sequence in fragments shorter than    3574 bp
  35% of sequence in fragments shorter than    4169 bp
  40% of sequence in fragments shorter than    4805 bp
  45% of sequence in fragments shorter than    5485 bp
  50% of sequence in fragments shorter than    6214 bp
  55% of sequence in fragments shorter than    7008 bp
  60% of sequence in fragments shorter than    7897 bp
  65% of sequence in fragments shorter than    8876 bp
  70% of sequence in fragments shorter than   10002 bp
  75% of sequence in fragments shorter than   11355 bp
  80% of sequence in fragments shorter than   13013 bp
  85% of sequence in fragments shorter than   15185 bp
  90% of sequence in fragments shorter than   18383 bp
  95% of sequence in fragments shorter than   24389 bp

# AvaI.PacI (CYCGRG+TTAATTAA) double digest, 1164 thousand fragments (1005714+159455 sites), 2558 bp average fragment length
   5% of sequence in fragments shorter than     848 bp
  10% of sequence in fragments shorter than    1377 bp
  15% of sequence in fragments shorter than    1871 bp
  20% of sequence in fragments shorter than    2355 bp
  25% of sequence in fragments shorter than    2850 bp
  30% of sequence in fragments shorter than    3366 bp
  35% of sequence in fragments shorter than    3910 bp
  40% of sequence in fragments shorter than    4484 bp
  45% of sequence in fragments shorter than    5105 bp
  50% of sequence in fragments shorter than    5764 bp
  55% of sequence in fragments shorter than    6495 bp
  60% of sequence in fragments shorter than    7311 bp
  65% of sequence in fragments shorter than    8238 bp
  70% of sequence in fragments shorter than    9317 bp
  75% of sequence in fragments shorter than   10590 bp
  80% of sequence in fragments shorter than   12163 bp
  85% of sequence in fragments shorter than   14281 bp
  90% of sequence in fragments shorter than   17412 bp
  95% of sequence in fragments shorter than   23963 bp

# BglII.HpaI (AGATCT+GTTAAC) double digest, 1163 thousand fragments (774732+389418 sites), 2560 bp average fragment length
   5% of sequence in fragments shorter than     893 bp
  10% of sequence in fragments shorter than    1351 bp
  15% of sequence in fragments shorter than    1729 bp
  20% of sequence in fragments shorter than    2098 bp
  25% of sequence in fragments shorter than    2462 bp
  30% of sequence in fragments shorter than    2828 bp
  35% of sequence in fragments shorter than    3204 bp
  40% of sequence in fragments shorter than    3586 bp
  45% of sequence in fragments shorter than    3981 bp
  50% of sequence in fragments shorter than    4400 bp
  55% of sequence in fragments shorter than    4852 bp
  60% of sequence in fragments shorter than    5348 bp
  65% of sequence in fragments shorter than    5904 bp
  70% of sequence in fragments shorter than    6546 bp
  75% of sequence in fragments shorter than    7298 bp
  80% of sequence in fragments shorter than    8227 bp
  85% of sequence in fragments shorter than    9437 bp
  90% of sequence in fragments shorter than   11246 bp
  95% of sequence in fragments shorter than   15085 bp

# AvaIII.KasI (ATGCAT+GGCGCC) double digest, 1163 thousand fragments (933357+230381 sites), 2561 bp average fragment length
   5% of sequence in fragments shorter than     909 bp
  10% of sequence in fragments shorter than    1384 bp
  15% of sequence in fragments shorter than    1797 bp
  20% of sequence in fragments shorter than    2172 bp
  25% of sequence in fragments shorter than    2547 bp
  30% of sequence in fragments shorter than    2916 bp
  35% of sequence in fragments shorter than    3297 bp
  40% of sequence in fragments shorter than    3693 bp
  45% of sequence in fragments shorter than    4108 bp
  50% of sequence in fragments shorter than    4553 bp
  55% of sequence in fragments shorter than    5021 bp
  60% of sequence in fragments shorter than    5538 bp
  65% of sequence in fragments shorter than    6101 bp
  70% of sequence in fragments shorter than    6738 bp
  75% of sequence in fragments shorter than    7489 bp
  80% of sequence in fragments shorter than    8389 bp
  85% of sequence in fragments shorter than    9563 bp
  90% of sequence in fragments shorter than   11252 bp
  95% of sequence in fragments shorter than   14446 bp

# AvaI.Hpy99I (CYCGRG+CGWCG) double digest, 1163 thousand fragments (1005714+157917 sites), 2561 bp average fragment length
   5% of sequence in fragments shorter than     824 bp
  10% of sequence in fragments shorter than    1390 bp
  15% of sequence in fragments shorter than    1935 bp
  20% of sequence in fragments shorter than    2487 bp
  25% of sequence in fragments shorter than    3061 bp
  30% of sequence in fragments shorter than    3678 bp
  35% of sequence in fragments shorter than    4330 bp
  40% of sequence in fragments shorter than    5043 bp
  45% of sequence in fragments shorter than    5812 bp
  50% of sequence in fragments shorter than    6670 bp
  55% of sequence in fragments shorter than    7625 bp
  60% of sequence in fragments shorter than    8708 bp
  65% of sequence in fragments shorter than    9947 bp
  70% of sequence in fragments shorter than   11378 bp
  75% of sequence in fragments shorter than   13126 bp
  80% of sequence in fragments shorter than   15283 bp
  85% of sequence in fragments shorter than   18162 bp
  90% of sequence in fragments shorter than   22445 bp
  95% of sequence in fragments shorter than   31070 bp

# BsrBI.StuI (CCGCTC+AGGCCT) double digest, 1160 thousand fragments (235523+925729 sites), 2567 bp average fragment length
   5% of sequence in fragments shorter than     752 bp
  10% of sequence in fragments shorter than    1300 bp
  15% of sequence in fragments shorter than    1801 bp
  20% of sequence in fragments shorter than    2288 bp
  25% of sequence in fragments shorter than    2796 bp
  30% of sequence in fragments shorter than    3310 bp
  35% of sequence in fragments shorter than    3838 bp
  40% of sequence in fragments shorter than    4389 bp
  45% of sequence in fragments shorter than    4976 bp
  50% of sequence in fragments shorter than    5597 bp
  55% of sequence in fragments shorter than    6270 bp
  60% of sequence in fragments shorter than    7008 bp
  65% of sequence in fragments shorter than    7825 bp
  70% of sequence in fragments shorter than    8750 bp
  75% of sequence in fragments shorter than    9836 bp
  80% of sequence in fragments shorter than   11141 bp
  85% of sequence in fragments shorter than   12782 bp
  90% of sequence in fragments shorter than   15098 bp
  95% of sequence in fragments shorter than   19083 bp

# BsaAI.EcoRV (YACGTR+GATATC) double digest, 1159 thousand fragments (713801+446473 sites), 2569 bp average fragment length
   5% of sequence in fragments shorter than     914 bp
  10% of sequence in fragments shorter than    1371 bp
  15% of sequence in fragments shorter than    1781 bp
  20% of sequence in fragments shorter than    2155 bp
  25% of sequence in fragments shorter than    2507 bp
  30% of sequence in fragments shorter than    2878 bp
  35% of sequence in fragments shorter than    3257 bp
  40% of sequence in fragments shorter than    3644 bp
  45% of sequence in fragments shorter than    4055 bp
  50% of sequence in fragments shorter than    4485 bp
  55% of sequence in fragments shorter than    4943 bp
  60% of sequence in fragments shorter than    5443 bp
  65% of sequence in fragments shorter than    6000 bp
  70% of sequence in fragments shorter than    6623 bp
  75% of sequence in fragments shorter than    7344 bp
  80% of sequence in fragments shorter than    8223 bp
  85% of sequence in fragments shorter than    9340 bp
  90% of sequence in fragments shorter than   10893 bp
  95% of sequence in fragments shorter than   13712 bp

# AccI.PspXI (GTMKAC+VCTCGAGB) double digest, 1159 thousand fragments (1101063+59193 sites), 2569 bp average fragment length
   5% of sequence in fragments shorter than     912 bp
  10% of sequence in fragments shorter than    1374 bp
  15% of sequence in fragments shorter than    1770 bp
  20% of sequence in fragments shorter than    2143 bp
  25% of sequence in fragments shorter than    2511 bp
  30% of sequence in fragments shorter than    2870 bp
  35% of sequence in fragments shorter than    3246 bp
  40% of sequence in fragments shorter than    3627 bp
  45% of sequence in fragments shorter than    4032 bp
  50% of sequence in fragments shorter than    4458 bp
  55% of sequence in fragments shorter than    4913 bp
  60% of sequence in fragments shorter than    5407 bp
  65% of sequence in fragments shorter than    5936 bp
  70% of sequence in fragments shorter than    6505 bp
  75% of sequence in fragments shorter than    7225 bp
  80% of sequence in fragments shorter than    8094 bp
  85% of sequence in fragments shorter than    9220 bp
  90% of sequence in fragments shorter than   10827 bp
  95% of sequence in fragments shorter than   13691 bp

# AvaIII.SwaI (ATGCAT+ATTTAAAT) double digest, 1159 thousand fragments (933357+226602 sites), 2569 bp average fragment length
   5% of sequence in fragments shorter than     897 bp
  10% of sequence in fragments shorter than    1374 bp
  15% of sequence in fragments shorter than    1792 bp
  20% of sequence in fragments shorter than    2180 bp
  25% of sequence in fragments shorter than    2569 bp
  30% of sequence in fragments shorter than    2960 bp
  35% of sequence in fragments shorter than    3364 bp
  40% of sequence in fragments shorter than    3788 bp
  45% of sequence in fragments shorter than    4236 bp
  50% of sequence in fragments shorter than    4724 bp
  55% of sequence in fragments shorter than    5251 bp
  60% of sequence in fragments shorter than    5846 bp
  65% of sequence in fragments shorter than    6514 bp
  70% of sequence in fragments shorter than    7283 bp
  75% of sequence in fragments shorter than    8190 bp
  80% of sequence in fragments shorter than    9306 bp
  85% of sequence in fragments shorter than   10841 bp
  90% of sequence in fragments shorter than   13136 bp
  95% of sequence in fragments shorter than   18120 bp

# NcoI.SpeI (CCATGG+ACTAGT) double digest, 1159 thousand fragments (759594+400286 sites), 2570 bp average fragment length
   5% of sequence in fragments shorter than     904 bp
  10% of sequence in fragments shorter than    1379 bp
  15% of sequence in fragments shorter than    1780 bp
  20% of sequence in fragments shorter than    2159 bp
  25% of sequence in fragments shorter than    2532 bp
  30% of sequence in fragments shorter than    2903 bp
  35% of sequence in fragments shorter than    3285 bp
  40% of sequence in fragments shorter than    3679 bp
  45% of sequence in fragments shorter than    4090 bp
  50% of sequence in fragments shorter than    4504 bp
  55% of sequence in fragments shorter than    4964 bp
  60% of sequence in fragments shorter than    5462 bp
  65% of sequence in fragments shorter than    6009 bp
  70% of sequence in fragments shorter than    6632 bp
  75% of sequence in fragments shorter than    7370 bp
  80% of sequence in fragments shorter than    8269 bp
  85% of sequence in fragments shorter than    9420 bp
  90% of sequence in fragments shorter than   11099 bp
  95% of sequence in fragments shorter than   14289 bp

# AvrII.MfeI (CCTAGG+CAATTG) double digest, 1158 thousand fragments (594956+564303 sites), 2571 bp average fragment length
   5% of sequence in fragments shorter than     892 bp
  10% of sequence in fragments shorter than    1371 bp
  15% of sequence in fragments shorter than    1781 bp
  20% of sequence in fragments shorter than    2153 bp
  25% of sequence in fragments shorter than    2524 bp
  30% of sequence in fragments shorter than    2896 bp
  35% of sequence in fragments shorter than    3268 bp
  40% of sequence in fragments shorter than    3655 bp
  45% of sequence in fragments shorter than    4059 bp
  50% of sequence in fragments shorter than    4478 bp
  55% of sequence in fragments shorter than    4926 bp
  60% of sequence in fragments shorter than    5410 bp
  65% of sequence in fragments shorter than    5948 bp
  70% of sequence in fragments shorter than    6557 bp
  75% of sequence in fragments shorter than    7266 bp
  80% of sequence in fragments shorter than    8126 bp
  85% of sequence in fragments shorter than    9248 bp
  90% of sequence in fragments shorter than   10815 bp
  95% of sequence in fragments shorter than   13787 bp

# HindIII.SexAI (AAGCTT+ACCWGGT) double digest, 1157 thousand fragments (860365+297900 sites), 2573 bp average fragment length
   5% of sequence in fragments shorter than     901 bp
  10% of sequence in fragments shorter than    1369 bp
  15% of sequence in fragments shorter than    1771 bp
  20% of sequence in fragments shorter than    2113 bp
  25% of sequence in fragments shorter than    2486 bp
  30% of sequence in fragments shorter than    2849 bp
  35% of sequence in fragments shorter than    3218 bp
  40% of sequence in fragments shorter than    3603 bp
  45% of sequence in fragments shorter than    4000 bp
  50% of sequence in fragments shorter than    4418 bp
  55% of sequence in fragments shorter than    4866 bp
  60% of sequence in fragments shorter than    5358 bp
  65% of sequence in fragments shorter than    5901 bp
  70% of sequence in fragments shorter than    6522 bp
  75% of sequence in fragments shorter than    7243 bp
  80% of sequence in fragments shorter than    8119 bp
  85% of sequence in fragments shorter than    9251 bp
  90% of sequence in fragments shorter than   10865 bp
  95% of sequence in fragments shorter than   13861 bp

# ApaI.BciVI (GGGCCC+GTATCC) double digest, 1156 thousand fragments (460339+696093 sites), 2577 bp average fragment length
   5% of sequence in fragments shorter than     891 bp
  10% of sequence in fragments shorter than    1379 bp
  15% of sequence in fragments shorter than    1800 bp
  20% of sequence in fragments shorter than    2194 bp
  25% of sequence in fragments shorter than    2592 bp
  30% of sequence in fragments shorter than    2997 bp
  35% of sequence in fragments shorter than    3406 bp
  40% of sequence in fragments shorter than    3830 bp
  45% of sequence in fragments shorter than    4269 bp
  50% of sequence in fragments shorter than    4741 bp
  55% of sequence in fragments shorter than    5250 bp
  60% of sequence in fragments shorter than    5806 bp
  65% of sequence in fragments shorter than    6428 bp
  70% of sequence in fragments shorter than    7128 bp
  75% of sequence in fragments shorter than    7960 bp
  80% of sequence in fragments shorter than    8967 bp
  85% of sequence in fragments shorter than   10262 bp
  90% of sequence in fragments shorter than   12112 bp
  95% of sequence in fragments shorter than   15583 bp

# AatII.PpuMI (GACGTC+RGGWCCY) double digest, 1155 thousand fragments (71089+1085138 sites), 2578 bp average fragment length
   5% of sequence in fragments shorter than     835 bp
  10% of sequence in fragments shorter than    1357 bp
  15% of sequence in fragments shorter than    1836 bp
  20% of sequence in fragments shorter than    2295 bp
  25% of sequence in fragments shorter than    2765 bp
  30% of sequence in fragments shorter than    3238 bp
  35% of sequence in fragments shorter than    3689 bp
  40% of sequence in fragments shorter than    4196 bp
  45% of sequence in fragments shorter than    4753 bp
  50% of sequence in fragments shorter than    5355 bp
  55% of sequence in fragments shorter than    6012 bp
  60% of sequence in fragments shorter than    6739 bp
  65% of sequence in fragments shorter than    7558 bp
  70% of sequence in fragments shorter than    8494 bp
  75% of sequence in fragments shorter than    9616 bp
  80% of sequence in fragments shorter than   10970 bp
  85% of sequence in fragments shorter than   12786 bp
  90% of sequence in fragments shorter than   15488 bp
  95% of sequence in fragments shorter than   20985 bp

# KasI.StuI (GGCGCC+AGGCCT) double digest, 1155 thousand fragments (230381+925729 sites), 2578 bp average fragment length
   5% of sequence in fragments shorter than     762 bp
  10% of sequence in fragments shorter than    1316 bp
  15% of sequence in fragments shorter than    1793 bp
  20% of sequence in fragments shorter than    2251 bp
  25% of sequence in fragments shorter than    2738 bp
  30% of sequence in fragments shorter than    3227 bp
  35% of sequence in fragments shorter than    3723 bp
  40% of sequence in fragments shorter than    4248 bp
  45% of sequence in fragments shorter than    4799 bp
  50% of sequence in fragments shorter than    5387 bp
  55% of sequence in fragments shorter than    6019 bp
  60% of sequence in fragments shorter than    6724 bp
  65% of sequence in fragments shorter than    7499 bp
  70% of sequence in fragments shorter than    8376 bp
  75% of sequence in fragments shorter than    9410 bp
  80% of sequence in fragments shorter than   10652 bp
  85% of sequence in fragments shorter than   12246 bp
  90% of sequence in fragments shorter than   14450 bp
  95% of sequence in fragments shorter than   18294 bp

# AatII.PvuII (GACGTC+CAGCTG) double digest, 1154 thousand fragments (71089+1084260 sites), 2580 bp average fragment length
   5% of sequence in fragments shorter than     867 bp
  10% of sequence in fragments shorter than    1352 bp
  15% of sequence in fragments shorter than    1789 bp
  20% of sequence in fragments shorter than    2219 bp
  25% of sequence in fragments shorter than    2640 bp
  30% of sequence in fragments shorter than    3055 bp
  35% of sequence in fragments shorter than    3502 bp
  40% of sequence in fragments shorter than    3972 bp
  45% of sequence in fragments shorter than    4479 bp
  50% of sequence in fragments shorter than    5019 bp
  55% of sequence in fragments shorter than    5608 bp
  60% of sequence in fragments shorter than    6247 bp
  65% of sequence in fragments shorter than    6956 bp
  70% of sequence in fragments shorter than    7767 bp
  75% of sequence in fragments shorter than    8730 bp
  80% of sequence in fragments shorter than    9944 bp
  85% of sequence in fragments shorter than   11527 bp
  90% of sequence in fragments shorter than   13841 bp
  95% of sequence in fragments shorter than   18421 bp

# AccI.AgeI (GTMKAC+ACCGGT) double digest, 1153 thousand fragments (1101063+53386 sites), 2582 bp average fragment length
   5% of sequence in fragments shorter than     917 bp
  10% of sequence in fragments shorter than    1380 bp
  15% of sequence in fragments shorter than    1779 bp
  20% of sequence in fragments shorter than    2148 bp
  25% of sequence in fragments shorter than    2517 bp
  30% of sequence in fragments shorter than    2880 bp
  35% of sequence in fragments shorter than    3259 bp
  40% of sequence in fragments shorter than    3645 bp
  45% of sequence in fragments shorter than    4053 bp
  50% of sequence in fragments shorter than    4458 bp
  55% of sequence in fragments shorter than    4919 bp
  60% of sequence in fragments shorter than    5423 bp
  65% of sequence in fragments shorter than    5969 bp
  70% of sequence in fragments shorter than    6580 bp
  75% of sequence in fragments shorter than    7317 bp
  80% of sequence in fragments shorter than    8208 bp
  85% of sequence in fragments shorter than    9357 bp
  90% of sequence in fragments shorter than   11011 bp
  95% of sequence in fragments shorter than   14018 bp

# SacI.SphI (GAGCTC+GCATGC) double digest, 1153 thousand fragments (603323+550952 sites), 2582 bp average fragment length
   5% of sequence in fragments shorter than     889 bp
  10% of sequence in fragments shorter than    1350 bp
  15% of sequence in fragments shorter than    1757 bp
  20% of sequence in fragments shorter than    2145 bp
  25% of sequence in fragments shorter than    2531 bp
  30% of sequence in fragments shorter than    2922 bp
  35% of sequence in fragments shorter than    3322 bp
  40% of sequence in fragments shorter than    3736 bp
  45% of sequence in fragments shorter than    4172 bp
  50% of sequence in fragments shorter than    4634 bp
  55% of sequence in fragments shorter than    5135 bp
  60% of sequence in fragments shorter than    5675 bp
  65% of sequence in fragments shorter than    6284 bp
  70% of sequence in fragments shorter than    6973 bp
  75% of sequence in fragments shorter than    7794 bp
  80% of sequence in fragments shorter than    8794 bp
  85% of sequence in fragments shorter than   10113 bp
  90% of sequence in fragments shorter than   12040 bp
  95% of sequence in fragments shorter than   15710 bp

# StuI.SwaI (AGGCCT+ATTTAAAT) double digest, 1151 thousand fragments (925729+226602 sites), 2587 bp average fragment length
   5% of sequence in fragments shorter than     812 bp
  10% of sequence in fragments shorter than    1349 bp
  15% of sequence in fragments shorter than    1805 bp
  20% of sequence in fragments shorter than    2228 bp
  25% of sequence in fragments shorter than    2667 bp
  30% of sequence in fragments shorter than    3099 bp
  35% of sequence in fragments shorter than    3537 bp
  40% of sequence in fragments shorter than    3993 bp
  45% of sequence in fragments shorter than    4461 bp
  50% of sequence in fragments shorter than    4960 bp
  55% of sequence in fragments shorter than    5495 bp
  60% of sequence in fragments shorter than    6075 bp
  65% of sequence in fragments shorter than    6727 bp
  70% of sequence in fragments shorter than    7451 bp
  75% of sequence in fragments shorter than    8286 bp
  80% of sequence in fragments shorter than    9276 bp
  85% of sequence in fragments shorter than   10540 bp
  90% of sequence in fragments shorter than   12285 bp
  95% of sequence in fragments shorter than   15204 bp

# PpuMI.SacII (RGGWCCY+CCGCGG) double digest, 1151 thousand fragments (1085138+66901 sites), 2587 bp average fragment length
   5% of sequence in fragments shorter than     837 bp
  10% of sequence in fragments shorter than    1385 bp
  15% of sequence in fragments shorter than    1889 bp
  20% of sequence in fragments shorter than    2376 bp
  25% of sequence in fragments shorter than    2871 bp
  30% of sequence in fragments shorter than    3367 bp
  35% of sequence in fragments shorter than    3834 bp
  40% of sequence in fragments shorter than    4379 bp
  45% of sequence in fragments shorter than    4966 bp
  50% of sequence in fragments shorter than    5597 bp
  55% of sequence in fragments shorter than    6291 bp
  60% of sequence in fragments shorter than    7061 bp
  65% of sequence in fragments shorter than    7922 bp
  70% of sequence in fragments shorter than    8916 bp
  75% of sequence in fragments shorter than   10098 bp
  80% of sequence in fragments shorter than   11543 bp
  85% of sequence in fragments shorter than   13473 bp
  90% of sequence in fragments shorter than   16361 bp
  95% of sequence in fragments shorter than   22381 bp

# BglII.SapI (AGATCT+GCTCTTC) double digest, 1151 thousand fragments (774732+377161 sites), 2587 bp average fragment length
   5% of sequence in fragments shorter than     887 bp
  10% of sequence in fragments shorter than    1360 bp
  15% of sequence in fragments shorter than    1764 bp
  20% of sequence in fragments shorter than    2145 bp
  25% of sequence in fragments shorter than    2518 bp
  30% of sequence in fragments shorter than    2891 bp
  35% of sequence in fragments shorter than    3276 bp
  40% of sequence in fragments shorter than    3668 bp
  45% of sequence in fragments shorter than    4074 bp
  50% of sequence in fragments shorter than    4500 bp
  55% of sequence in fragments shorter than    4954 bp
  60% of sequence in fragments shorter than    5454 bp
  65% of sequence in fragments shorter than    6011 bp
  70% of sequence in fragments shorter than    6649 bp
  75% of sequence in fragments shorter than    7398 bp
  80% of sequence in fragments shorter than    8294 bp
  85% of sequence in fragments shorter than    9473 bp
  90% of sequence in fragments shorter than   11194 bp
  95% of sequence in fragments shorter than   14691 bp

# BglII.SmaI (AGATCT+CCCGGG) double digest, 1151 thousand fragments (774732+376940 sites), 2588 bp average fragment length
   5% of sequence in fragments shorter than     889 bp
  10% of sequence in fragments shorter than    1361 bp
  15% of sequence in fragments shorter than    1752 bp
  20% of sequence in fragments shorter than    2133 bp
  25% of sequence in fragments shorter than    2510 bp
  30% of sequence in fragments shorter than    2889 bp
  35% of sequence in fragments shorter than    3278 bp
  40% of sequence in fragments shorter than    3672 bp
  45% of sequence in fragments shorter than    4087 bp
  50% of sequence in fragments shorter than    4521 bp
  55% of sequence in fragments shorter than    4987 bp
  60% of sequence in fragments shorter than    5498 bp
  65% of sequence in fragments shorter than    6070 bp
  70% of sequence in fragments shorter than    6719 bp
  75% of sequence in fragments shorter than    7481 bp
  80% of sequence in fragments shorter than    8408 bp
  85% of sequence in fragments shorter than    9627 bp
  90% of sequence in fragments shorter than   11413 bp
  95% of sequence in fragments shorter than   15080 bp

# BceAI.EcoRV (ACGGC+GATATC) double digest, 1151 thousand fragments (705178+446473 sites), 2588 bp average fragment length
   5% of sequence in fragments shorter than     883 bp
  10% of sequence in fragments shorter than    1382 bp
  15% of sequence in fragments shorter than    1819 bp
  20% of sequence in fragments shorter than    2230 bp
  25% of sequence in fragments shorter than    2626 bp
  30% of sequence in fragments shorter than    3031 bp
  35% of sequence in fragments shorter than    3449 bp
  40% of sequence in fragments shorter than    3883 bp
  45% of sequence in fragments shorter than    4333 bp
  50% of sequence in fragments shorter than    4812 bp
  55% of sequence in fragments shorter than    5322 bp
  60% of sequence in fragments shorter than    5880 bp
  65% of sequence in fragments shorter than    6496 bp
  70% of sequence in fragments shorter than    7200 bp
  75% of sequence in fragments shorter than    8012 bp
  80% of sequence in fragments shorter than    8986 bp
  85% of sequence in fragments shorter than   10227 bp
  90% of sequence in fragments shorter than   11991 bp
  95% of sequence in fragments shorter than   15162 bp

# PvuII.SacII (CAGCTG+CCGCGG) double digest, 1150 thousand fragments (1084260+66901 sites), 2589 bp average fragment length
   5% of sequence in fragments shorter than     866 bp
  10% of sequence in fragments shorter than    1369 bp
  15% of sequence in fragments shorter than    1823 bp
  20% of sequence in fragments shorter than    2270 bp
  25% of sequence in fragments shorter than    2708 bp
  30% of sequence in fragments shorter than    3147 bp
  35% of sequence in fragments shorter than    3613 bp
  40% of sequence in fragments shorter than    4107 bp
  45% of sequence in fragments shorter than    4634 bp
  50% of sequence in fragments shorter than    5200 bp
  55% of sequence in fragments shorter than    5812 bp
  60% of sequence in fragments shorter than    6477 bp
  65% of sequence in fragments shorter than    7215 bp
  70% of sequence in fragments shorter than    8071 bp
  75% of sequence in fragments shorter than    9079 bp
  80% of sequence in fragments shorter than   10345 bp
  85% of sequence in fragments shorter than   11980 bp
  90% of sequence in fragments shorter than   14444 bp
  95% of sequence in fragments shorter than   19295 bp

# AclI.BspHI (AACGTT+TCATGA) double digest, 1150 thousand fragments (172706+978290 sites), 2590 bp average fragment length
   5% of sequence in fragments shorter than     910 bp
  10% of sequence in fragments shorter than    1378 bp
  15% of sequence in fragments shorter than    1787 bp
  20% of sequence in fragments shorter than    2145 bp
  25% of sequence in fragments shorter than    2509 bp
  30% of sequence in fragments shorter than    2871 bp
  35% of sequence in fragments shorter than    3239 bp
  40% of sequence in fragments shorter than    3625 bp
  45% of sequence in fragments shorter than    4032 bp
  50% of sequence in fragments shorter than    4464 bp
  55% of sequence in fragments shorter than    4927 bp
  60% of sequence in fragments shorter than    5434 bp
  65% of sequence in fragments shorter than    5994 bp
  70% of sequence in fragments shorter than    6631 bp
  75% of sequence in fragments shorter than    7381 bp
  80% of sequence in fragments shorter than    8288 bp
  85% of sequence in fragments shorter than    9485 bp
  90% of sequence in fragments shorter than   11231 bp
  95% of sequence in fragments shorter than   14561 bp

# HpaI.NcoI (GTTAAC+CCATGG) double digest, 1148 thousand fragments (389418+759594 sites), 2594 bp average fragment length
   5% of sequence in fragments shorter than     913 bp
  10% of sequence in fragments shorter than    1377 bp
  15% of sequence in fragments shorter than    1778 bp
  20% of sequence in fragments shorter than    2159 bp
  25% of sequence in fragments shorter than    2531 bp
  30% of sequence in fragments shorter than    2902 bp
  35% of sequence in fragments shorter than    3257 bp
  40% of sequence in fragments shorter than    3645 bp
  45% of sequence in fragments shorter than    4056 bp
  50% of sequence in fragments shorter than    4485 bp
  55% of sequence in fragments shorter than    4952 bp
  60% of sequence in fragments shorter than    5453 bp
  65% of sequence in fragments shorter than    6008 bp
  70% of sequence in fragments shorter than    6637 bp
  75% of sequence in fragments shorter than    7382 bp
  80% of sequence in fragments shorter than    8276 bp
  85% of sequence in fragments shorter than    9459 bp
  90% of sequence in fragments shorter than   11153 bp
  95% of sequence in fragments shorter than   14462 bp

# SexAI.XbaI (ACCWGGT+TCTAGA) double digest, 1148 thousand fragments (297900+850998 sites), 2594 bp average fragment length
   5% of sequence in fragments shorter than     884 bp
  10% of sequence in fragments shorter than    1373 bp
  15% of sequence in fragments shorter than    1750 bp
  20% of sequence in fragments shorter than    2135 bp
  25% of sequence in fragments shorter than    2499 bp
  30% of sequence in fragments shorter than    2879 bp
  35% of sequence in fragments shorter than    3258 bp
  40% of sequence in fragments shorter than    3639 bp
  45% of sequence in fragments shorter than    4047 bp
  50% of sequence in fragments shorter than    4478 bp
  55% of sequence in fragments shorter than    4937 bp
  60% of sequence in fragments shorter than    5435 bp
  65% of sequence in fragments shorter than    5982 bp
  70% of sequence in fragments shorter than    6608 bp
  75% of sequence in fragments shorter than    7343 bp
  80% of sequence in fragments shorter than    8221 bp
  85% of sequence in fragments shorter than    9352 bp
  90% of sequence in fragments shorter than   10947 bp
  95% of sequence in fragments shorter than   13791 bp

# HindIII.KpnI (AAGCTT+GGTACC) double digest, 1147 thousand fragments (860365+287974 sites), 2596 bp average fragment length
   5% of sequence in fragments shorter than     923 bp
  10% of sequence in fragments shorter than    1373 bp
  15% of sequence in fragments shorter than    1747 bp
  20% of sequence in fragments shorter than    2124 bp
  25% of sequence in fragments shorter than    2505 bp
  30% of sequence in fragments shorter than    2881 bp
  35% of sequence in fragments shorter than    3261 bp
  40% of sequence in fragments shorter than    3662 bp
  45% of sequence in fragments shorter than    4077 bp
  50% of sequence in fragments shorter than    4512 bp
  55% of sequence in fragments shorter than    4983 bp
  60% of sequence in fragments shorter than    5495 bp
  65% of sequence in fragments shorter than    6070 bp
  70% of sequence in fragments shorter than    6720 bp
  75% of sequence in fragments shorter than    7473 bp
  80% of sequence in fragments shorter than    8397 bp
  85% of sequence in fragments shorter than    9597 bp
  90% of sequence in fragments shorter than   11331 bp
  95% of sequence in fragments shorter than   14614 bp

# HindIII.NheI (AAGCTT+GCTAGC) double digest, 1147 thousand fragments (860365+287384 sites), 2597 bp average fragment length
   5% of sequence in fragments shorter than     909 bp
  10% of sequence in fragments shorter than    1333 bp
  15% of sequence in fragments shorter than    1732 bp
  20% of sequence in fragments shorter than    2118 bp
  25% of sequence in fragments shorter than    2506 bp
  30% of sequence in fragments shorter than    2891 bp
  35% of sequence in fragments shorter than    3278 bp
  40% of sequence in fragments shorter than    3684 bp
  45% of sequence in fragments shorter than    4102 bp
  50% of sequence in fragments shorter than    4548 bp
  55% of sequence in fragments shorter than    5027 bp
  60% of sequence in fragments shorter than    5553 bp
  65% of sequence in fragments shorter than    6141 bp
  70% of sequence in fragments shorter than    6815 bp
  75% of sequence in fragments shorter than    7589 bp
  80% of sequence in fragments shorter than    8549 bp
  85% of sequence in fragments shorter than    9784 bp
  90% of sequence in fragments shorter than   11544 bp
  95% of sequence in fragments shorter than   14901 bp

# SacI.ScaI (GAGCTC+AGTACT) double digest, 1146 thousand fragments (603323+543793 sites), 2598 bp average fragment length
   5% of sequence in fragments shorter than     912 bp
  10% of sequence in fragments shorter than    1372 bp
  15% of sequence in fragments shorter than    1772 bp
  20% of sequence in fragments shorter than    2147 bp
  25% of sequence in fragments shorter than    2515 bp
  30% of sequence in fragments shorter than    2880 bp
  35% of sequence in fragments shorter than    3257 bp
  40% of sequence in fragments shorter than    3644 bp
  45% of sequence in fragments shorter than    4052 bp
  50% of sequence in fragments shorter than    4478 bp
  55% of sequence in fragments shorter than    4932 bp
  60% of sequence in fragments shorter than    5422 bp
  65% of sequence in fragments shorter than    5978 bp
  70% of sequence in fragments shorter than    6598 bp
  75% of sequence in fragments shorter than    7323 bp
  80% of sequence in fragments shorter than    8199 bp
  85% of sequence in fragments shorter than    9332 bp
  90% of sequence in fragments shorter than   10965 bp
  95% of sequence in fragments shorter than   14137 bp

# AvrII.SphI (CCTAGG+GCATGC) double digest, 1145 thousand fragments (594956+550952 sites), 2601 bp average fragment length
   5% of sequence in fragments shorter than     898 bp
  10% of sequence in fragments shorter than    1382 bp
  15% of sequence in fragments shorter than    1794 bp
  20% of sequence in fragments shorter than    2179 bp
  25% of sequence in fragments shorter than    2559 bp
  30% of sequence in fragments shorter than    2939 bp
  35% of sequence in fragments shorter than    3321 bp
  40% of sequence in fragments shorter than    3717 bp
  45% of sequence in fragments shorter than    4129 bp
  50% of sequence in fragments shorter than    4566 bp
  55% of sequence in fragments shorter than    5039 bp
  60% of sequence in fragments shorter than    5554 bp
  65% of sequence in fragments shorter than    6127 bp
  70% of sequence in fragments shorter than    6780 bp
  75% of sequence in fragments shorter than    7538 bp
  80% of sequence in fragments shorter than    8465 bp
  85% of sequence in fragments shorter than    9694 bp
  90% of sequence in fragments shorter than   11521 bp
  95% of sequence in fragments shorter than   15112 bp

# EcoRI.SexAI (GAATTC+ACCWGGT) double digest, 1144 thousand fragments (847401+297900 sites), 2603 bp average fragment length
   5% of sequence in fragments shorter than     876 bp
  10% of sequence in fragments shorter than    1382 bp
  15% of sequence in fragments shorter than    1817 bp
  20% of sequence in fragments shorter than    2216 bp
  25% of sequence in fragments shorter than    2600 bp
  30% of sequence in fragments shorter than    2978 bp
  35% of sequence in fragments shorter than    3364 bp
  40% of sequence in fragments shorter than    3752 bp
  45% of sequence in fragments shorter than    4166 bp
  50% of sequence in fragments shorter than    4606 bp
  55% of sequence in fragments shorter than    5078 bp
  60% of sequence in fragments shorter than    5589 bp
  65% of sequence in fragments shorter than    6151 bp
  70% of sequence in fragments shorter than    6780 bp
  75% of sequence in fragments shorter than    7515 bp
  80% of sequence in fragments shorter than    8389 bp
  85% of sequence in fragments shorter than    9513 bp
  90% of sequence in fragments shorter than   11090 bp
  95% of sequence in fragments shorter than   13869 bp

# PpuMI.PspXI (RGGWCCY+VCTCGAGB) double digest, 1143 thousand fragments (1085138+59193 sites), 2605 bp average fragment length
   5% of sequence in fragments shorter than     843 bp
  10% of sequence in fragments shorter than    1371 bp
  15% of sequence in fragments shorter than    1857 bp
  20% of sequence in fragments shorter than    2322 bp
  25% of sequence in fragments shorter than    2799 bp
  30% of sequence in fragments shorter than    3277 bp
  35% of sequence in fragments shorter than    3738 bp
  40% of sequence in fragments shorter than    4254 bp
  45% of sequence in fragments shorter than    4821 bp
  50% of sequence in fragments shorter than    5433 bp
  55% of sequence in fragments shorter than    6096 bp
  60% of sequence in fragments shorter than    6835 bp
  65% of sequence in fragments shorter than    7668 bp
  70% of sequence in fragments shorter than    8623 bp
  75% of sequence in fragments shorter than    9767 bp
  80% of sequence in fragments shorter than   11145 bp
  85% of sequence in fragments shorter than   13015 bp
  90% of sequence in fragments shorter than   15768 bp
  95% of sequence in fragments shorter than   21402 bp

# PspXI.PvuII (VCTCGAGB+CAGCTG) double digest, 1143 thousand fragments (59193+1084260 sites), 2606 bp average fragment length
   5% of sequence in fragments shorter than     873 bp
  10% of sequence in fragments shorter than    1365 bp
  15% of sequence in fragments shorter than    1807 bp
  20% of sequence in fragments shorter than    2240 bp
  25% of sequence in fragments shorter than    2666 bp
  30% of sequence in fragments shorter than    3090 bp
  35% of sequence in fragments shorter than    3544 bp
  40% of sequence in fragments shorter than    4020 bp
  45% of sequence in fragments shorter than    4533 bp
  50% of sequence in fragments shorter than    5082 bp
  55% of sequence in fragments shorter than    5682 bp
  60% of sequence in fragments shorter than    6335 bp
  65% of sequence in fragments shorter than    7054 bp
  70% of sequence in fragments shorter than    7882 bp
  75% of sequence in fragments shorter than    8854 bp
  80% of sequence in fragments shorter than   10094 bp
  85% of sequence in fragments shorter than   11695 bp
  90% of sequence in fragments shorter than   14065 bp
  95% of sequence in fragments shorter than   18706 bp

# EciI.HgaI (GGCGGA+GACGC) double digest, 1142 thousand fragments (571273+571889 sites), 2607 bp average fragment length
   5% of sequence in fragments shorter than     843 bp
  10% of sequence in fragments shorter than    1406 bp
  15% of sequence in fragments shorter than    1931 bp
  20% of sequence in fragments shorter than    2461 bp
  25% of sequence in fragments shorter than    2996 bp
  30% of sequence in fragments shorter than    3561 bp
  35% of sequence in fragments shorter than    4163 bp
  40% of sequence in fragments shorter than    4819 bp
  45% of sequence in fragments shorter than    5526 bp
  50% of sequence in fragments shorter than    6304 bp
  55% of sequence in fragments shorter than    7156 bp
  60% of sequence in fragments shorter than    8101 bp
  65% of sequence in fragments shorter than    9195 bp
  70% of sequence in fragments shorter than   10486 bp
  75% of sequence in fragments shorter than   11999 bp
  80% of sequence in fragments shorter than   13928 bp
  85% of sequence in fragments shorter than   16414 bp
  90% of sequence in fragments shorter than   20129 bp
  95% of sequence in fragments shorter than   27159 bp

# AccI.PmeI (GTMKAC+GTTTAAAC) double digest, 1142 thousand fragments (1101063+42094 sites), 2607 bp average fragment length
   5% of sequence in fragments shorter than     921 bp
  10% of sequence in fragments shorter than    1384 bp
  15% of sequence in fragments shorter than    1793 bp
  20% of sequence in fragments shorter than    2174 bp
  25% of sequence in fragments shorter than    2548 bp
  30% of sequence in fragments shorter than    2917 bp
  35% of sequence in fragments shorter than    3302 bp
  40% of sequence in fragments shorter than    3694 bp
  45% of sequence in fragments shorter than    4110 bp
  50% of sequence in fragments shorter than    4554 bp
  55% of sequence in fragments shorter than    5024 bp
  60% of sequence in fragments shorter than    5534 bp
  65% of sequence in fragments shorter than    6055 bp
  70% of sequence in fragments shorter than    6682 bp
  75% of sequence in fragments shorter than    7440 bp
  80% of sequence in fragments shorter than    8361 bp
  85% of sequence in fragments shorter than    9559 bp
  90% of sequence in fragments shorter than   11279 bp
  95% of sequence in fragments shorter than   14481 bp

# BciVI.EcoRV (GTATCC+GATATC) double digest, 1142 thousand fragments (696093+446473 sites), 2609 bp average fragment length
   5% of sequence in fragments shorter than     902 bp
  10% of sequence in fragments shorter than    1353 bp
  15% of sequence in fragments shorter than    1770 bp
  20% of sequence in fragments shorter than    2152 bp
  25% of sequence in fragments shorter than    2535 bp
  30% of sequence in fragments shorter than    2917 bp
  35% of sequence in fragments shorter than    3304 bp
  40% of sequence in fragments shorter than    3708 bp
  45% of sequence in fragments shorter than    4129 bp
  50% of sequence in fragments shorter than    4574 bp
  55% of sequence in fragments shorter than    5060 bp
  60% of sequence in fragments shorter than    5593 bp
  65% of sequence in fragments shorter than    6181 bp
  70% of sequence in fragments shorter than    6853 bp
  75% of sequence in fragments shorter than    7641 bp
  80% of sequence in fragments shorter than    8635 bp
  85% of sequence in fragments shorter than    9904 bp
  90% of sequence in fragments shorter than   11766 bp
  95% of sequence in fragments shorter than   15275 bp

# BamHI.BglII (GGATCC+AGATCT) double digest, 1140 thousand fragments (365999+774732 sites), 2613 bp average fragment length
   5% of sequence in fragments shorter than     923 bp
  10% of sequence in fragments shorter than    1394 bp
  15% of sequence in fragments shorter than    1778 bp
  20% of sequence in fragments shorter than    2131 bp
  25% of sequence in fragments shorter than    2498 bp
  30% of sequence in fragments shorter than    2846 bp
  35% of sequence in fragments shorter than    3217 bp
  40% of sequence in fragments shorter than    3599 bp
  45% of sequence in fragments shorter than    3999 bp
  50% of sequence in fragments shorter than    4422 bp
  55% of sequence in fragments shorter than    4882 bp
  60% of sequence in fragments shorter than    5375 bp
  65% of sequence in fragments shorter than    5925 bp
  70% of sequence in fragments shorter than    6553 bp
  75% of sequence in fragments shorter than    7287 bp
  80% of sequence in fragments shorter than    8167 bp
  85% of sequence in fragments shorter than    9312 bp
  90% of sequence in fragments shorter than   10976 bp
  95% of sequence in fragments shorter than   14314 bp

# AflII.ApaLI (CTTAAG+GTGCAC) double digest, 1139 thousand fragments (643284+496255 sites), 2616 bp average fragment length
   5% of sequence in fragments shorter than     937 bp
  10% of sequence in fragments shorter than    1406 bp
  15% of sequence in fragments shorter than    1808 bp
  20% of sequence in fragments shorter than    2145 bp
  25% of sequence in fragments shorter than    2513 bp
  30% of sequence in fragments shorter than    2879 bp
  35% of sequence in fragments shorter than    3254 bp
  40% of sequence in fragments shorter than    3631 bp
  45% of sequence in fragments shorter than    4025 bp
  50% of sequence in fragments shorter than    4435 bp
  55% of sequence in fragments shorter than    4876 bp
  60% of sequence in fragments shorter than    5361 bp
  65% of sequence in fragments shorter than    5897 bp
  70% of sequence in fragments shorter than    6506 bp
  75% of sequence in fragments shorter than    7215 bp
  80% of sequence in fragments shorter than    8073 bp
  85% of sequence in fragments shorter than    9201 bp
  90% of sequence in fragments shorter than   10830 bp
  95% of sequence in fragments shorter than   14054 bp

# BclI.SpeI (TGATCA+ACTAGT) double digest, 1138 thousand fragments (738785+400286 sites), 2617 bp average fragment length
   5% of sequence in fragments shorter than     922 bp
  10% of sequence in fragments shorter than    1387 bp
  15% of sequence in fragments shorter than    1777 bp
  20% of sequence in fragments shorter than    2129 bp
  25% of sequence in fragments shorter than    2496 bp
  30% of sequence in fragments shorter than    2861 bp
  35% of sequence in fragments shorter than    3238 bp
  40% of sequence in fragments shorter than    3629 bp
  45% of sequence in fragments shorter than    4039 bp
  50% of sequence in fragments shorter than    4466 bp
  55% of sequence in fragments shorter than    4933 bp
  60% of sequence in fragments shorter than    5443 bp
  65% of sequence in fragments shorter than    6019 bp
  70% of sequence in fragments shorter than    6676 bp
  75% of sequence in fragments shorter than    7440 bp
  80% of sequence in fragments shorter than    8390 bp
  85% of sequence in fragments shorter than    9650 bp
  90% of sequence in fragments shorter than   11528 bp
  95% of sequence in fragments shorter than   15462 bp

# KpnI.XbaI (GGTACC+TCTAGA) double digest, 1138 thousand fragments (287974+850998 sites), 2617 bp average fragment length
   5% of sequence in fragments shorter than     862 bp
  10% of sequence in fragments shorter than    1358 bp
  15% of sequence in fragments shorter than    1749 bp
  20% of sequence in fragments shorter than    2142 bp
  25% of sequence in fragments shorter than    2540 bp
  30% of sequence in fragments shorter than    2934 bp
  35% of sequence in fragments shorter than    3328 bp
  40% of sequence in fragments shorter than    3734 bp
  45% of sequence in fragments shorter than    4170 bp
  50% of sequence in fragments shorter than    4626 bp
  55% of sequence in fragments shorter than    5121 bp
  60% of sequence in fragments shorter than    5654 bp
  65% of sequence in fragments shorter than    6249 bp
  70% of sequence in fragments shorter than    6916 bp
  75% of sequence in fragments shorter than    7694 bp
  80% of sequence in fragments shorter than    8641 bp
  85% of sequence in fragments shorter than    9853 bp
  90% of sequence in fragments shorter than   11565 bp
  95% of sequence in fragments shorter than   14610 bp

# BsrBI.NdeI (CCGCTC+CATATG) double digest, 1138 thousand fragments (235523+903360 sites), 2617 bp average fragment length
   5% of sequence in fragments shorter than     903 bp
  10% of sequence in fragments shorter than    1375 bp
  15% of sequence in fragments shorter than    1813 bp
  20% of sequence in fragments shorter than    2213 bp
  25% of sequence in fragments shorter than    2619 bp
  30% of sequence in fragments shorter than    3018 bp
  35% of sequence in fragments shorter than    3420 bp
  40% of sequence in fragments shorter than    3840 bp
  45% of sequence in fragments shorter than    4278 bp
  50% of sequence in fragments shorter than    4735 bp
  55% of sequence in fragments shorter than    5225 bp
  60% of sequence in fragments shorter than    5756 bp
  65% of sequence in fragments shorter than    6340 bp
  70% of sequence in fragments shorter than    7006 bp
  75% of sequence in fragments shorter than    7785 bp
  80% of sequence in fragments shorter than    8723 bp
  85% of sequence in fragments shorter than    9964 bp
  90% of sequence in fragments shorter than   11712 bp
  95% of sequence in fragments shorter than   15027 bp

# AvrII.ScaI (CCTAGG+AGTACT) double digest, 1138 thousand fragments (594956+543793 sites), 2617 bp average fragment length
   5% of sequence in fragments shorter than     909 bp
  10% of sequence in fragments shorter than    1387 bp
  15% of sequence in fragments shorter than    1791 bp
  20% of sequence in fragments shorter than    2171 bp
  25% of sequence in fragments shorter than    2536 bp
  30% of sequence in fragments shorter than    2903 bp
  35% of sequence in fragments shorter than    3279 bp
  40% of sequence in fragments shorter than    3668 bp
  45% of sequence in fragments shorter than    4070 bp
  50% of sequence in fragments shorter than    4497 bp
  55% of sequence in fragments shorter than    4947 bp
  60% of sequence in fragments shorter than    5443 bp
  65% of sequence in fragments shorter than    5989 bp
  70% of sequence in fragments shorter than    6613 bp
  75% of sequence in fragments shorter than    7333 bp
  80% of sequence in fragments shorter than    8207 bp
  85% of sequence in fragments shorter than    9357 bp
  90% of sequence in fragments shorter than   11039 bp
  95% of sequence in fragments shorter than   14447 bp

# AgeI.PpuMI (ACCGGT+RGGWCCY) double digest, 1138 thousand fragments (53386+1085138 sites), 2618 bp average fragment length
   5% of sequence in fragments shorter than     849 bp
  10% of sequence in fragments shorter than    1369 bp
  15% of sequence in fragments shorter than    1857 bp
  20% of sequence in fragments shorter than    2325 bp
  25% of sequence in fragments shorter than    2782 bp
  30% of sequence in fragments shorter than    3262 bp
  35% of sequence in fragments shorter than    3747 bp
  40% of sequence in fragments shorter than    4264 bp
  45% of sequence in fragments shorter than    4830 bp
  50% of sequence in fragments shorter than    5440 bp
  55% of sequence in fragments shorter than    6107 bp
  60% of sequence in fragments shorter than    6850 bp
  65% of sequence in fragments shorter than    7679 bp
  70% of sequence in fragments shorter than    8632 bp
  75% of sequence in fragments shorter than    9767 bp
  80% of sequence in fragments shorter than   11139 bp
  85% of sequence in fragments shorter than   13001 bp
  90% of sequence in fragments shorter than   15719 bp
  95% of sequence in fragments shorter than   21443 bp

# NheI.XbaI (GCTAGC+TCTAGA) double digest, 1137 thousand fragments (287384+850998 sites), 2618 bp average fragment length
   5% of sequence in fragments shorter than     860 bp
  10% of sequence in fragments shorter than    1360 bp
  15% of sequence in fragments shorter than    1774 bp
  20% of sequence in fragments shorter than    2183 bp
  25% of sequence in fragments shorter than    2586 bp
  30% of sequence in fragments shorter than    2958 bp
  35% of sequence in fragments shorter than    3366 bp
  40% of sequence in fragments shorter than    3788 bp
  45% of sequence in fragments shorter than    4231 bp
  50% of sequence in fragments shorter than    4698 bp
  55% of sequence in fragments shorter than    5203 bp
  60% of sequence in fragments shorter than    5753 bp
  65% of sequence in fragments shorter than    6362 bp
  70% of sequence in fragments shorter than    7053 bp
  75% of sequence in fragments shorter than    7856 bp
  80% of sequence in fragments shorter than    8823 bp
  85% of sequence in fragments shorter than   10087 bp
  90% of sequence in fragments shorter than   11898 bp
  95% of sequence in fragments shorter than   15223 bp

# AvaI.SnaBI (CYCGRG+TACGTA) double digest, 1137 thousand fragments (1005714+132299 sites), 2619 bp average fragment length
   5% of sequence in fragments shorter than     858 bp
  10% of sequence in fragments shorter than    1391 bp
  15% of sequence in fragments shorter than    1898 bp
  20% of sequence in fragments shorter than    2400 bp
  25% of sequence in fragments shorter than    2909 bp
  30% of sequence in fragments shorter than    3452 bp
  35% of sequence in fragments shorter than    4021 bp
  40% of sequence in fragments shorter than    4622 bp
  45% of sequence in fragments shorter than    5272 bp
  50% of sequence in fragments shorter than    5976 bp
  55% of sequence in fragments shorter than    6766 bp
  60% of sequence in fragments shorter than    7640 bp
  65% of sequence in fragments shorter than    8639 bp
  70% of sequence in fragments shorter than    9790 bp
  75% of sequence in fragments shorter than   11145 bp
  80% of sequence in fragments shorter than   12832 bp
  85% of sequence in fragments shorter than   15132 bp
  90% of sequence in fragments shorter than   18458 bp
  95% of sequence in fragments shorter than   25090 bp

# BspHI.PacI (TCATGA+TTAATTAA) double digest, 1137 thousand fragments (978290+159455 sites), 2620 bp average fragment length
   5% of sequence in fragments shorter than     926 bp
  10% of sequence in fragments shorter than    1407 bp
  15% of sequence in fragments shorter than    1826 bp
  20% of sequence in fragments shorter than    2192 bp
  25% of sequence in fragments shorter than    2564 bp
  30% of sequence in fragments shorter than    2943 bp
  35% of sequence in fragments shorter than    3323 bp
  40% of sequence in fragments shorter than    3726 bp
  45% of sequence in fragments shorter than    4150 bp
  50% of sequence in fragments shorter than    4593 bp
  55% of sequence in fragments shorter than    5070 bp
  60% of sequence in fragments shorter than    5595 bp
  65% of sequence in fragments shorter than    6180 bp
  70% of sequence in fragments shorter than    6847 bp
  75% of sequence in fragments shorter than    7646 bp
  80% of sequence in fragments shorter than    8625 bp
  85% of sequence in fragments shorter than    9911 bp
  90% of sequence in fragments shorter than   11863 bp
  95% of sequence in fragments shorter than   16006 bp

# AgeI.PvuII (ACCGGT+CAGCTG) double digest, 1137 thousand fragments (53386+1084260 sites), 2620 bp average fragment length
   5% of sequence in fragments shorter than     880 bp
  10% of sequence in fragments shorter than    1369 bp
  15% of sequence in fragments shorter than    1810 bp
  20% of sequence in fragments shorter than    2244 bp
  25% of sequence in fragments shorter than    2674 bp
  30% of sequence in fragments shorter than    3098 bp
  35% of sequence in fragments shorter than    3550 bp
  40% of sequence in fragments shorter than    4027 bp
  45% of sequence in fragments shorter than    4534 bp
  50% of sequence in fragments shorter than    5078 bp
  55% of sequence in fragments shorter than    5669 bp
  60% of sequence in fragments shorter than    6315 bp
  65% of sequence in fragments shorter than    7029 bp
  70% of sequence in fragments shorter than    7848 bp
  75% of sequence in fragments shorter than    8815 bp
  80% of sequence in fragments shorter than   10040 bp
  85% of sequence in fragments shorter than   11642 bp
  90% of sequence in fragments shorter than   13964 bp
  95% of sequence in fragments shorter than   18602 bp

# NcoI.SapI (CCATGG+GCTCTTC) double digest, 1136 thousand fragments (759594+377161 sites), 2622 bp average fragment length
   5% of sequence in fragments shorter than     897 bp
  10% of sequence in fragments shorter than    1381 bp
  15% of sequence in fragments shorter than    1801 bp
  20% of sequence in fragments shorter than    2203 bp
  25% of sequence in fragments shorter than    2594 bp
  30% of sequence in fragments shorter than    2991 bp
  35% of sequence in fragments shorter than    3396 bp
  40% of sequence in fragments shorter than    3780 bp
  45% of sequence in fragments shorter than    4211 bp
  50% of sequence in fragments shorter than    4684 bp
  55% of sequence in fragments shorter than    5192 bp
  60% of sequence in fragments shorter than    5745 bp
  65% of sequence in fragments shorter than    6369 bp
  70% of sequence in fragments shorter than    7091 bp
  75% of sequence in fragments shorter than    7941 bp
  80% of sequence in fragments shorter than    8985 bp
  85% of sequence in fragments shorter than   10337 bp
  90% of sequence in fragments shorter than   12291 bp
  95% of sequence in fragments shorter than   16197 bp

# NcoI.SmaI (CCATGG+CCCGGG) double digest, 1136 thousand fragments (759594+376940 sites), 2622 bp average fragment length
   5% of sequence in fragments shorter than     878 bp
  10% of sequence in fragments shorter than    1372 bp
  15% of sequence in fragments shorter than    1806 bp
  20% of sequence in fragments shorter than    2228 bp
  25% of sequence in fragments shorter than    2650 bp
  30% of sequence in fragments shorter than    3075 bp
  35% of sequence in fragments shorter than    3515 bp
  40% of sequence in fragments shorter than    3971 bp
  45% of sequence in fragments shorter than    4454 bp
  50% of sequence in fragments shorter than    4951 bp
  55% of sequence in fragments shorter than    5502 bp
  60% of sequence in fragments shorter than    6094 bp
  65% of sequence in fragments shorter than    6776 bp
  70% of sequence in fragments shorter than    7559 bp
  75% of sequence in fragments shorter than    8499 bp
  80% of sequence in fragments shorter than    9651 bp
  85% of sequence in fragments shorter than   11163 bp
  90% of sequence in fragments shorter than   13364 bp
  95% of sequence in fragments shorter than   17825 bp

# BspHI.Hpy99I (TCATGA+CGWCG) double digest, 1135 thousand fragments (978290+157917 sites), 2623 bp average fragment length
   5% of sequence in fragments shorter than     933 bp
  10% of sequence in fragments shorter than    1424 bp
  15% of sequence in fragments shorter than    1845 bp
  20% of sequence in fragments shorter than    2215 bp
  25% of sequence in fragments shorter than    2587 bp
  30% of sequence in fragments shorter than    2967 bp
  35% of sequence in fragments shorter than    3344 bp
  40% of sequence in fragments shorter than    3737 bp
  45% of sequence in fragments shorter than    4144 bp
  50% of sequence in fragments shorter than    4559 bp
  55% of sequence in fragments shorter than    5023 bp
  60% of sequence in fragments shorter than    5528 bp
  65% of sequence in fragments shorter than    6085 bp
  70% of sequence in fragments shorter than    6719 bp
  75% of sequence in fragments shorter than    7452 bp
  80% of sequence in fragments shorter than    8355 bp
  85% of sequence in fragments shorter than    9503 bp
  90% of sequence in fragments shorter than   11169 bp
  95% of sequence in fragments shorter than   14365 bp

# HgaI.MfeI (GACGC+CAATTG) double digest, 1135 thousand fragments (571889+564303 sites), 2623 bp average fragment length
   5% of sequence in fragments shorter than     912 bp
  10% of sequence in fragments shorter than    1416 bp
  15% of sequence in fragments shorter than    1868 bp
  20% of sequence in fragments shorter than    2272 bp
  25% of sequence in fragments shorter than    2680 bp
  30% of sequence in fragments shorter than    3081 bp
  35% of sequence in fragments shorter than    3489 bp
  40% of sequence in fragments shorter than    3916 bp
  45% of sequence in fragments shorter than    4350 bp
  50% of sequence in fragments shorter than    4816 bp
  55% of sequence in fragments shorter than    5323 bp
  60% of sequence in fragments shorter than    5869 bp
  65% of sequence in fragments shorter than    6480 bp
  70% of sequence in fragments shorter than    7160 bp
  75% of sequence in fragments shorter than    7963 bp
  80% of sequence in fragments shorter than    8933 bp
  85% of sequence in fragments shorter than   10175 bp
  90% of sequence in fragments shorter than   11913 bp
  95% of sequence in fragments shorter than   15054 bp

# EciI.MfeI (GGCGGA+CAATTG) double digest, 1135 thousand fragments (571273+564303 sites), 2625 bp average fragment length
   5% of sequence in fragments shorter than     899 bp
  10% of sequence in fragments shorter than    1398 bp
  15% of sequence in fragments shorter than    1838 bp
  20% of sequence in fragments shorter than    2235 bp
  25% of sequence in fragments shorter than    2632 bp
  30% of sequence in fragments shorter than    3030 bp
  35% of sequence in fragments shorter than    3437 bp
  40% of sequence in fragments shorter than    3861 bp
  45% of sequence in fragments shorter than    4303 bp
  50% of sequence in fragments shorter than    4769 bp
  55% of sequence in fragments shorter than    5272 bp
  60% of sequence in fragments shorter than    5820 bp
  65% of sequence in fragments shorter than    6424 bp
  70% of sequence in fragments shorter than    7110 bp
  75% of sequence in fragments shorter than    7920 bp
  80% of sequence in fragments shorter than    8901 bp
  85% of sequence in fragments shorter than   10172 bp
  90% of sequence in fragments shorter than   12000 bp
  95% of sequence in fragments shorter than   15396 bp

# EcoRI.KpnI (GAATTC+GGTACC) double digest, 1134 thousand fragments (847401+287974 sites), 2625 bp average fragment length
   5% of sequence in fragments shorter than     905 bp
  10% of sequence in fragments shorter than    1390 bp
  15% of sequence in fragments shorter than    1811 bp
  20% of sequence in fragments shorter than    2212 bp
  25% of sequence in fragments shorter than    2605 bp
  30% of sequence in fragments shorter than    2993 bp
  35% of sequence in fragments shorter than    3387 bp
  40% of sequence in fragments shorter than    3795 bp
  45% of sequence in fragments shorter than    4223 bp
  50% of sequence in fragments shorter than    4678 bp
  55% of sequence in fragments shorter than    5162 bp
  60% of sequence in fragments shorter than    5692 bp
  65% of sequence in fragments shorter than    6276 bp
  70% of sequence in fragments shorter than    6940 bp
  75% of sequence in fragments shorter than    7703 bp
  80% of sequence in fragments shorter than    8623 bp
  85% of sequence in fragments shorter than    9797 bp
  90% of sequence in fragments shorter than   11456 bp
  95% of sequence in fragments shorter than   14454 bp

# EcoRI.NheI (GAATTC+GCTAGC) double digest, 1134 thousand fragments (847401+287384 sites), 2627 bp average fragment length
   5% of sequence in fragments shorter than     894 bp
  10% of sequence in fragments shorter than    1402 bp
  15% of sequence in fragments shorter than    1822 bp
  20% of sequence in fragments shorter than    2226 bp
  25% of sequence in fragments shorter than    2623 bp
  30% of sequence in fragments shorter than    2986 bp
  35% of sequence in fragments shorter than    3388 bp
  40% of sequence in fragments shorter than    3810 bp
  45% of sequence in fragments shorter than    4248 bp
  50% of sequence in fragments shorter than    4706 bp
  55% of sequence in fragments shorter than    5200 bp
  60% of sequence in fragments shorter than    5737 bp
  65% of sequence in fragments shorter than    6328 bp
  70% of sequence in fragments shorter than    6993 bp
  75% of sequence in fragments shorter than    7774 bp
  80% of sequence in fragments shorter than    8720 bp
  85% of sequence in fragments shorter than    9916 bp
  90% of sequence in fragments shorter than   11649 bp
  95% of sequence in fragments shorter than   14744 bp

# FnuDII.SpeI (CGCG+ACTAGT) double digest, 1133 thousand fragments (733940+400286 sites), 2628 bp average fragment length
   5% of sequence in fragments shorter than    1076 bp
  10% of sequence in fragments shorter than    1757 bp
  15% of sequence in fragments shorter than    2344 bp
  20% of sequence in fragments shorter than    2893 bp
  25% of sequence in fragments shorter than    3442 bp
  30% of sequence in fragments shorter than    3997 bp
  35% of sequence in fragments shorter than    4565 bp
  40% of sequence in fragments shorter than    5136 bp
  45% of sequence in fragments shorter than    5718 bp
  50% of sequence in fragments shorter than    6375 bp
  55% of sequence in fragments shorter than    7077 bp
  60% of sequence in fragments shorter than    7848 bp
  65% of sequence in fragments shorter than    8705 bp
  70% of sequence in fragments shorter than    9688 bp
  75% of sequence in fragments shorter than   10847 bp
  80% of sequence in fragments shorter than   12240 bp
  85% of sequence in fragments shorter than   14035 bp
  90% of sequence in fragments shorter than   16602 bp
  95% of sequence in fragments shorter than   21634 bp

# KasI.NdeI (GGCGCC+CATATG) double digest, 1133 thousand fragments (230381+903360 sites), 2629 bp average fragment length
   5% of sequence in fragments shorter than     899 bp
  10% of sequence in fragments shorter than    1371 bp
  15% of sequence in fragments shorter than    1806 bp
  20% of sequence in fragments shorter than    2215 bp
  25% of sequence in fragments shorter than    2619 bp
  30% of sequence in fragments shorter than    3014 bp
  35% of sequence in fragments shorter than    3417 bp
  40% of sequence in fragments shorter than    3835 bp
  45% of sequence in fragments shorter than    4250 bp
  50% of sequence in fragments shorter than    4696 bp
  55% of sequence in fragments shorter than    5179 bp
  60% of sequence in fragments shorter than    5707 bp
  65% of sequence in fragments shorter than    6289 bp
  70% of sequence in fragments shorter than    6952 bp
  75% of sequence in fragments shorter than    7735 bp
  80% of sequence in fragments shorter than    8689 bp
  85% of sequence in fragments shorter than    9936 bp
  90% of sequence in fragments shorter than   11761 bp
  95% of sequence in fragments shorter than   15399 bp

# AvaI.NaeI (CYCGRG+GCCGGC) double digest, 1131 thousand fragments (1005714+125692 sites), 2634 bp average fragment length
   5% of sequence in fragments shorter than     846 bp
  10% of sequence in fragments shorter than    1429 bp
  15% of sequence in fragments shorter than    1990 bp
  20% of sequence in fragments shorter than    2564 bp
  25% of sequence in fragments shorter than    3165 bp
  30% of sequence in fragments shorter than    3814 bp
  35% of sequence in fragments shorter than    4501 bp
  40% of sequence in fragments shorter than    5249 bp
  45% of sequence in fragments shorter than    6057 bp
  50% of sequence in fragments shorter than    6965 bp
  55% of sequence in fragments shorter than    7974 bp
  60% of sequence in fragments shorter than    9110 bp
  65% of sequence in fragments shorter than   10415 bp
  70% of sequence in fragments shorter than   11930 bp
  75% of sequence in fragments shorter than   13775 bp
  80% of sequence in fragments shorter than   16087 bp
  85% of sequence in fragments shorter than   19133 bp
  90% of sequence in fragments shorter than   23860 bp
  95% of sequence in fragments shorter than   33676 bp

# NdeI.SwaI (CATATG+ATTTAAAT) double digest, 1129 thousand fragments (903360+226602 sites), 2638 bp average fragment length
   5% of sequence in fragments shorter than     879 bp
  10% of sequence in fragments shorter than    1345 bp
  15% of sequence in fragments shorter than    1782 bp
  20% of sequence in fragments shorter than    2203 bp
  25% of sequence in fragments shorter than    2623 bp
  30% of sequence in fragments shorter than    3040 bp
  35% of sequence in fragments shorter than    3473 bp
  40% of sequence in fragments shorter than    3930 bp
  45% of sequence in fragments shorter than    4411 bp
  50% of sequence in fragments shorter than    4915 bp
  55% of sequence in fragments shorter than    5460 bp
  60% of sequence in fragments shorter than    6073 bp
  65% of sequence in fragments shorter than    6771 bp
  70% of sequence in fragments shorter than    7591 bp
  75% of sequence in fragments shorter than    8598 bp
  80% of sequence in fragments shorter than    9876 bp
  85% of sequence in fragments shorter than   11635 bp
  90% of sequence in fragments shorter than   14560 bp
  95% of sequence in fragments shorter than   21612 bp

# BclI.HpaI (TGATCA+GTTAAC) double digest, 1127 thousand fragments (738785+389418 sites), 2642 bp average fragment length
   5% of sequence in fragments shorter than     925 bp
  10% of sequence in fragments shorter than    1387 bp
  15% of sequence in fragments shorter than    1790 bp
  20% of sequence in fragments shorter than    2175 bp
  25% of sequence in fragments shorter than    2550 bp
  30% of sequence in fragments shorter than    2916 bp
  35% of sequence in fragments shorter than    3294 bp
  40% of sequence in fragments shorter than    3687 bp
  45% of sequence in fragments shorter than    4092 bp
  50% of sequence in fragments shorter than    4528 bp
  55% of sequence in fragments shorter than    4994 bp
  60% of sequence in fragments shorter than    5505 bp
  65% of sequence in fragments shorter than    6080 bp
  70% of sequence in fragments shorter than    6734 bp
  75% of sequence in fragments shorter than    7500 bp
  80% of sequence in fragments shorter than    8453 bp
  85% of sequence in fragments shorter than    9702 bp
  90% of sequence in fragments shorter than   11592 bp
  95% of sequence in fragments shorter than   15587 bp

# PmeI.PpuMI (GTTTAAAC+RGGWCCY) double digest, 1126 thousand fragments (42094+1085138 sites), 2644 bp average fragment length
   5% of sequence in fragments shorter than     864 bp
  10% of sequence in fragments shorter than    1395 bp
  15% of sequence in fragments shorter than    1878 bp
  20% of sequence in fragments shorter than    2342 bp
  25% of sequence in fragments shorter than    2814 bp
  30% of sequence in fragments shorter than    3287 bp
  35% of sequence in fragments shorter than    3740 bp
  40% of sequence in fragments shorter than    4246 bp
  45% of sequence in fragments shorter than    4802 bp
  50% of sequence in fragments shorter than    5397 bp
  55% of sequence in fragments shorter than    6048 bp
  60% of sequence in fragments shorter than    6770 bp
  65% of sequence in fragments shorter than    7583 bp
  70% of sequence in fragments shorter than    8511 bp
  75% of sequence in fragments shorter than    9612 bp
  80% of sequence in fragments shorter than   10942 bp
  85% of sequence in fragments shorter than   12729 bp
  90% of sequence in fragments shorter than   15397 bp
  95% of sequence in fragments shorter than   20792 bp

# PmeI.PvuII (GTTTAAAC+CAGCTG) double digest, 1125 thousand fragments (42094+1084260 sites), 2646 bp average fragment length
   5% of sequence in fragments shorter than     890 bp
  10% of sequence in fragments shorter than    1385 bp
  15% of sequence in fragments shorter than    1833 bp
  20% of sequence in fragments shorter than    2266 bp
  25% of sequence in fragments shorter than    2691 bp
  30% of sequence in fragments shorter than    3113 bp
  35% of sequence in fragments shorter than    3562 bp
  40% of sequence in fragments shorter than    4035 bp
  45% of sequence in fragments shorter than    4538 bp
  50% of sequence in fragments shorter than    5076 bp
  55% of sequence in fragments shorter than    5664 bp
  60% of sequence in fragments shorter than    6299 bp
  65% of sequence in fragments shorter than    7002 bp
  70% of sequence in fragments shorter than    7817 bp
  75% of sequence in fragments shorter than    8759 bp
  80% of sequence in fragments shorter than    9953 bp
  85% of sequence in fragments shorter than   11501 bp
  90% of sequence in fragments shorter than   13745 bp
  95% of sequence in fragments shorter than   18208 bp

# ApaLI.HaeII (GTGCAC+RGCGCY) double digest, 1125 thousand fragments (496255+630101 sites), 2646 bp average fragment length
   5% of sequence in fragments shorter than     826 bp
  10% of sequence in fragments shorter than    1385 bp
  15% of sequence in fragments shorter than    1909 bp
  20% of sequence in fragments shorter than    2401 bp
  25% of sequence in fragments shorter than    2903 bp
  30% of sequence in fragments shorter than    3416 bp
  35% of sequence in fragments shorter than    3949 bp
  40% of sequence in fragments shorter than    4483 bp
  45% of sequence in fragments shorter than    5063 bp
  50% of sequence in fragments shorter than    5658 bp
  55% of sequence in fragments shorter than    6320 bp
  60% of sequence in fragments shorter than    7071 bp
  65% of sequence in fragments shorter than    7912 bp
  70% of sequence in fragments shorter than    8874 bp
  75% of sequence in fragments shorter than    9987 bp
  80% of sequence in fragments shorter than   11350 bp
  85% of sequence in fragments shorter than   13129 bp
  90% of sequence in fragments shorter than   15595 bp
  95% of sequence in fragments shorter than   19888 bp

# BamHI.NcoI (GGATCC+CCATGG) double digest, 1125 thousand fragments (365999+759594 sites), 2648 bp average fragment length
   5% of sequence in fragments shorter than     884 bp
  10% of sequence in fragments shorter than    1384 bp
  15% of sequence in fragments shorter than    1823 bp
  20% of sequence in fragments shorter than    2231 bp
  25% of sequence in fragments shorter than    2644 bp
  30% of sequence in fragments shorter than    3059 bp
  35% of sequence in fragments shorter than    3483 bp
  40% of sequence in fragments shorter than    3908 bp
  45% of sequence in fragments shorter than    4365 bp
  50% of sequence in fragments shorter than    4846 bp
  55% of sequence in fragments shorter than    5369 bp
  60% of sequence in fragments shorter than    5932 bp
  65% of sequence in fragments shorter than    6578 bp
  70% of sequence in fragments shorter than    7323 bp
  75% of sequence in fragments shorter than    8203 bp
  80% of sequence in fragments shorter than    9266 bp
  85% of sequence in fragments shorter than   10637 bp
  90% of sequence in fragments shorter than   12656 bp
  95% of sequence in fragments shorter than   16590 bp

# FnuDII.HpaI (CGCG+GTTAAC) double digest, 1122 thousand fragments (733940+389418 sites), 2653 bp average fragment length
   5% of sequence in fragments shorter than    1077 bp
  10% of sequence in fragments shorter than    1778 bp
  15% of sequence in fragments shorter than    2381 bp
  20% of sequence in fragments shorter than    2955 bp
  25% of sequence in fragments shorter than    3518 bp
  30% of sequence in fragments shorter than    4091 bp
  35% of sequence in fragments shorter than    4681 bp
  40% of sequence in fragments shorter than    5292 bp
  45% of sequence in fragments shorter than    5941 bp
  50% of sequence in fragments shorter than    6636 bp
  55% of sequence in fragments shorter than    7386 bp
  60% of sequence in fragments shorter than    8210 bp
  65% of sequence in fragments shorter than    9133 bp
  70% of sequence in fragments shorter than   10182 bp
  75% of sequence in fragments shorter than   11427 bp
  80% of sequence in fragments shorter than   12929 bp
  85% of sequence in fragments shorter than   14927 bp
  90% of sequence in fragments shorter than   17857 bp
  95% of sequence in fragments shorter than   23610 bp

# HgaI.SphI (GACGC+GCATGC) double digest, 1122 thousand fragments (571889+550952 sites), 2655 bp average fragment length
   5% of sequence in fragments shorter than     900 bp
  10% of sequence in fragments shorter than    1419 bp
  15% of sequence in fragments shorter than    1888 bp
  20% of sequence in fragments shorter than    2331 bp
  25% of sequence in fragments shorter than    2771 bp
  30% of sequence in fragments shorter than    3219 bp
  35% of sequence in fragments shorter than    3687 bp
  40% of sequence in fragments shorter than    4173 bp
  45% of sequence in fragments shorter than    4704 bp
  50% of sequence in fragments shorter than    5264 bp
  55% of sequence in fragments shorter than    5867 bp
  60% of sequence in fragments shorter than    6539 bp
  65% of sequence in fragments shorter than    7281 bp
  70% of sequence in fragments shorter than    8140 bp
  75% of sequence in fragments shorter than    9152 bp
  80% of sequence in fragments shorter than   10377 bp
  85% of sequence in fragments shorter than   12032 bp
  90% of sequence in fragments shorter than   14402 bp
  95% of sequence in fragments shorter than   18740 bp

# AccI.MluI (GTMKAC+ACGCGT) double digest, 1122 thousand fragments (1101063+21415 sites), 2655 bp average fragment length
   5% of sequence in fragments shorter than     942 bp
  10% of sequence in fragments shorter than    1411 bp
  15% of sequence in fragments shorter than    1831 bp
  20% of sequence in fragments shorter than    2219 bp
  25% of sequence in fragments shorter than    2597 bp
  30% of sequence in fragments shorter than    2973 bp
  35% of sequence in fragments shorter than    3365 bp
  40% of sequence in fragments shorter than    3763 bp
  45% of sequence in fragments shorter than    4183 bp
  50% of sequence in fragments shorter than    4631 bp
  55% of sequence in fragments shorter than    5108 bp
  60% of sequence in fragments shorter than    5611 bp
  65% of sequence in fragments shorter than    6133 bp
  70% of sequence in fragments shorter than    6781 bp
  75% of sequence in fragments shorter than    7534 bp
  80% of sequence in fragments shorter than    8457 bp
  85% of sequence in fragments shorter than    9651 bp
  90% of sequence in fragments shorter than   11362 bp
  95% of sequence in fragments shorter than   14480 bp

# EciI.SphI (GGCGGA+GCATGC) double digest, 1121 thousand fragments (571273+550952 sites), 2656 bp average fragment length
   5% of sequence in fragments shorter than     896 bp
  10% of sequence in fragments shorter than    1408 bp
  15% of sequence in fragments shorter than    1873 bp
  20% of sequence in fragments shorter than    2313 bp
  25% of sequence in fragments shorter than    2762 bp
  30% of sequence in fragments shorter than    3220 bp
  35% of sequence in fragments shorter than    3701 bp
  40% of sequence in fragments shorter than    4212 bp
  45% of sequence in fragments shorter than    4762 bp
  50% of sequence in fragments shorter than    5359 bp
  55% of sequence in fragments shorter than    6008 bp
  60% of sequence in fragments shorter than    6722 bp
  65% of sequence in fragments shorter than    7522 bp
  70% of sequence in fragments shorter than    8475 bp
  75% of sequence in fragments shorter than    9595 bp
  80% of sequence in fragments shorter than   11018 bp
  85% of sequence in fragments shorter than   12840 bp
  90% of sequence in fragments shorter than   15542 bp
  95% of sequence in fragments shorter than   20742 bp

# PpuMI.SalI (RGGWCCY+GTCGAC) double digest, 1116 thousand fragments (1085138+31396 sites), 2669 bp average fragment length
   5% of sequence in fragments shorter than     867 bp
  10% of sequence in fragments shorter than    1406 bp
  15% of sequence in fragments shorter than    1898 bp
  20% of sequence in fragments shorter than    2376 bp
  25% of sequence in fragments shorter than    2862 bp
  30% of sequence in fragments shorter than    3349 bp
  35% of sequence in fragments shorter than    3808 bp
  40% of sequence in fragments shorter than    4338 bp
  45% of sequence in fragments shorter than    4915 bp
  50% of sequence in fragments shorter than    5535 bp
  55% of sequence in fragments shorter than    6213 bp
  60% of sequence in fragments shorter than    6972 bp
  65% of sequence in fragments shorter than    7817 bp
  70% of sequence in fragments shorter than    8780 bp
  75% of sequence in fragments shorter than    9933 bp
  80% of sequence in fragments shorter than   11345 bp
  85% of sequence in fragments shorter than   13224 bp
  90% of sequence in fragments shorter than   16013 bp
  95% of sequence in fragments shorter than   21769 bp

# AccI.SgrAI (GTMKAC+CRCCGGYG) double digest, 1115 thousand fragments (1101063+15270 sites), 2670 bp average fragment length
   5% of sequence in fragments shorter than     946 bp
  10% of sequence in fragments shorter than    1418 bp
  15% of sequence in fragments shorter than    1839 bp
  20% of sequence in fragments shorter than    2230 bp
  25% of sequence in fragments shorter than    2609 bp
  30% of sequence in fragments shorter than    2988 bp
  35% of sequence in fragments shorter than    3382 bp
  40% of sequence in fragments shorter than    3782 bp
  45% of sequence in fragments shorter than    4207 bp
  50% of sequence in fragments shorter than    4654 bp
  55% of sequence in fragments shorter than    5136 bp
  60% of sequence in fragments shorter than    5639 bp
  65% of sequence in fragments shorter than    6162 bp
  70% of sequence in fragments shorter than    6822 bp
  75% of sequence in fragments shorter than    7581 bp
  80% of sequence in fragments shorter than    8517 bp
  85% of sequence in fragments shorter than    9725 bp
  90% of sequence in fragments shorter than   11438 bp
  95% of sequence in fragments shorter than   14620 bp

# BclI.SapI (TGATCA+GCTCTTC) double digest, 1115 thousand fragments (738785+377161 sites), 2671 bp average fragment length
   5% of sequence in fragments shorter than     927 bp
  10% of sequence in fragments shorter than    1392 bp
  15% of sequence in fragments shorter than    1804 bp
  20% of sequence in fragments shorter than    2190 bp
  25% of sequence in fragments shorter than    2567 bp
  30% of sequence in fragments shorter than    2940 bp
  35% of sequence in fragments shorter than    3317 bp
  40% of sequence in fragments shorter than    3715 bp
  45% of sequence in fragments shorter than    4123 bp
  50% of sequence in fragments shorter than    4556 bp
  55% of sequence in fragments shorter than    5016 bp
  60% of sequence in fragments shorter than    5522 bp
  65% of sequence in fragments shorter than    6079 bp
  70% of sequence in fragments shorter than    6725 bp
  75% of sequence in fragments shorter than    7469 bp
  80% of sequence in fragments shorter than    8391 bp
  85% of sequence in fragments shorter than    9558 bp
  90% of sequence in fragments shorter than   11293 bp
  95% of sequence in fragments shorter than   14704 bp

# AccI.NruI (GTMKAC+TCGCGA) double digest, 1115 thousand fragments (1101063+14872 sites), 2671 bp average fragment length
   5% of sequence in fragments shorter than     945 bp
  10% of sequence in fragments shorter than    1417 bp
  15% of sequence in fragments shorter than    1839 bp
  20% of sequence in fragments shorter than    2230 bp
  25% of sequence in fragments shorter than    2610 bp
  30% of sequence in fragments shorter than    2989 bp
  35% of sequence in fragments shorter than    3384 bp
  40% of sequence in fragments shorter than    3784 bp
  45% of sequence in fragments shorter than    4211 bp
  50% of sequence in fragments shorter than    4655 bp
  55% of sequence in fragments shorter than    5137 bp
  60% of sequence in fragments shorter than    5642 bp
  65% of sequence in fragments shorter than    6167 bp
  70% of sequence in fragments shorter than    6830 bp
  75% of sequence in fragments shorter than    7589 bp
  80% of sequence in fragments shorter than    8524 bp
  85% of sequence in fragments shorter than    9734 bp
  90% of sequence in fragments shorter than   11464 bp
  95% of sequence in fragments shorter than   14681 bp

# BclI.SmaI (TGATCA+CCCGGG) double digest, 1115 thousand fragments (738785+376940 sites), 2671 bp average fragment length
   5% of sequence in fragments shorter than     920 bp
  10% of sequence in fragments shorter than    1408 bp
  15% of sequence in fragments shorter than    1821 bp
  20% of sequence in fragments shorter than    2218 bp
  25% of sequence in fragments shorter than    2601 bp
  30% of sequence in fragments shorter than    2983 bp
  35% of sequence in fragments shorter than    3377 bp
  40% of sequence in fragments shorter than    3785 bp
  45% of sequence in fragments shorter than    4206 bp
  50% of sequence in fragments shorter than    4661 bp
  55% of sequence in fragments shorter than    5148 bp
  60% of sequence in fragments shorter than    5678 bp
  65% of sequence in fragments shorter than    6275 bp
  70% of sequence in fragments shorter than    6954 bp
  75% of sequence in fragments shorter than    7753 bp
  80% of sequence in fragments shorter than    8713 bp
  85% of sequence in fragments shorter than    9979 bp
  90% of sequence in fragments shorter than   11857 bp
  95% of sequence in fragments shorter than   15618 bp

# PvuII.SalI (CAGCTG+GTCGAC) double digest, 1115 thousand fragments (1084260+31396 sites), 2671 bp average fragment length
   5% of sequence in fragments shorter than     895 bp
  10% of sequence in fragments shorter than    1396 bp
  15% of sequence in fragments shorter than    1853 bp
  20% of sequence in fragments shorter than    2295 bp
  25% of sequence in fragments shorter than    2726 bp
  30% of sequence in fragments shorter than    3161 bp
  35% of sequence in fragments shorter than    3620 bp
  40% of sequence in fragments shorter than    4107 bp
  45% of sequence in fragments shorter than    4624 bp
  50% of sequence in fragments shorter than    5181 bp
  55% of sequence in fragments shorter than    5785 bp
  60% of sequence in fragments shorter than    6440 bp
  65% of sequence in fragments shorter than    7168 bp
  70% of sequence in fragments shorter than    8009 bp
  75% of sequence in fragments shorter than    8992 bp
  80% of sequence in fragments shorter than   10238 bp
  85% of sequence in fragments shorter than   11840 bp
  90% of sequence in fragments shorter than   14230 bp
  95% of sequence in fragments shorter than   18956 bp

# HgaI.ScaI (GACGC+AGTACT) double digest, 1115 thousand fragments (571889+543793 sites), 2672 bp average fragment length
   5% of sequence in fragments shorter than     930 bp
  10% of sequence in fragments shorter than    1444 bp
  15% of sequence in fragments shorter than    1896 bp
  20% of sequence in fragments shorter than    2326 bp
  25% of sequence in fragments shorter than    2739 bp
  30% of sequence in fragments shorter than    3146 bp
  35% of sequence in fragments shorter than    3572 bp
  40% of sequence in fragments shorter than    4013 bp
  45% of sequence in fragments shorter than    4472 bp
  50% of sequence in fragments shorter than    4964 bp
  55% of sequence in fragments shorter than    5493 bp
  60% of sequence in fragments shorter than    6071 bp
  65% of sequence in fragments shorter than    6722 bp
  70% of sequence in fragments shorter than    7443 bp
  75% of sequence in fragments shorter than    8291 bp
  80% of sequence in fragments shorter than    9309 bp
  85% of sequence in fragments shorter than   10641 bp
  90% of sequence in fragments shorter than   12562 bp
  95% of sequence in fragments shorter than   16210 bp

# MfeI.SphI (CAATTG+GCATGC) double digest, 1114 thousand fragments (564303+550952 sites), 2673 bp average fragment length
   5% of sequence in fragments shorter than     937 bp
  10% of sequence in fragments shorter than    1419 bp
  15% of sequence in fragments shorter than    1836 bp
  20% of sequence in fragments shorter than    2207 bp
  25% of sequence in fragments shorter than    2580 bp
  30% of sequence in fragments shorter than    2953 bp
  35% of sequence in fragments shorter than    3326 bp
  40% of sequence in fragments shorter than    3719 bp
  45% of sequence in fragments shorter than    4124 bp
  50% of sequence in fragments shorter than    4548 bp
  55% of sequence in fragments shorter than    5005 bp
  60% of sequence in fragments shorter than    5501 bp
  65% of sequence in fragments shorter than    6046 bp
  70% of sequence in fragments shorter than    6664 bp
  75% of sequence in fragments shorter than    7396 bp
  80% of sequence in fragments shorter than    8268 bp
  85% of sequence in fragments shorter than    9402 bp
  90% of sequence in fragments shorter than   11020 bp
  95% of sequence in fragments shorter than   14001 bp

# AccI.PvuI (GTMKAC+CGATCG) double digest, 1114 thousand fragments (1101063+14186 sites), 2673 bp average fragment length
   5% of sequence in fragments shorter than     946 bp
  10% of sequence in fragments shorter than    1420 bp
  15% of sequence in fragments shorter than    1842 bp
  20% of sequence in fragments shorter than    2234 bp
  25% of sequence in fragments shorter than    2614 bp
  30% of sequence in fragments shorter than    2993 bp
  35% of sequence in fragments shorter than    3389 bp
  40% of sequence in fragments shorter than    3791 bp
  45% of sequence in fragments shorter than    4218 bp
  50% of sequence in fragments shorter than    4664 bp
  55% of sequence in fragments shorter than    5147 bp
  60% of sequence in fragments shorter than    5653 bp
  65% of sequence in fragments shorter than    6182 bp
  70% of sequence in fragments shorter than    6847 bp
  75% of sequence in fragments shorter than    7607 bp
  80% of sequence in fragments shorter than    8548 bp
  85% of sequence in fragments shorter than    9764 bp
  90% of sequence in fragments shorter than   11498 bp
  95% of sequence in fragments shorter than   14751 bp

# EciI.ScaI (GGCGGA+AGTACT) double digest, 1114 thousand fragments (571273+543793 sites), 2673 bp average fragment length
   5% of sequence in fragments shorter than     912 bp
  10% of sequence in fragments shorter than    1413 bp
  15% of sequence in fragments shorter than    1856 bp
  20% of sequence in fragments shorter than    2274 bp
  25% of sequence in fragments shorter than    2688 bp
  30% of sequence in fragments shorter than    3107 bp
  35% of sequence in fragments shorter than    3544 bp
  40% of sequence in fragments shorter than    3998 bp
  45% of sequence in fragments shorter than    4480 bp
  50% of sequence in fragments shorter than    5002 bp
  55% of sequence in fragments shorter than    5567 bp
  60% of sequence in fragments shorter than    6187 bp
  65% of sequence in fragments shorter than    6881 bp
  70% of sequence in fragments shorter than    7674 bp
  75% of sequence in fragments shorter than    8601 bp
  80% of sequence in fragments shorter than    9752 bp
  85% of sequence in fragments shorter than   11284 bp
  90% of sequence in fragments shorter than   13547 bp
  95% of sequence in fragments shorter than   17930 bp

# AccI.FseI (GTMKAC+GGCCGGCC) double digest, 1114 thousand fragments (1101063+13662 sites), 2674 bp average fragment length
   5% of sequence in fragments shorter than     948 bp
  10% of sequence in fragments shorter than    1421 bp
  15% of sequence in fragments shorter than    1842 bp
  20% of sequence in fragments shorter than    2233 bp
  25% of sequence in fragments shorter than    2613 bp
  30% of sequence in fragments shorter than    2991 bp
  35% of sequence in fragments shorter than    3387 bp
  40% of sequence in fragments shorter than    3786 bp
  45% of sequence in fragments shorter than    4212 bp
  50% of sequence in fragments shorter than    4656 bp
  55% of sequence in fragments shorter than    5139 bp
  60% of sequence in fragments shorter than    5643 bp
  65% of sequence in fragments shorter than    6166 bp
  70% of sequence in fragments shorter than    6825 bp
  75% of sequence in fragments shorter than    7581 bp
  80% of sequence in fragments shorter than    8512 bp
  85% of sequence in fragments shorter than    9717 bp
  90% of sequence in fragments shorter than   11432 bp
  95% of sequence in fragments shorter than   14564 bp

# BsaAI.SpeI (YACGTR+ACTAGT) double digest, 1113 thousand fragments (713801+400286 sites), 2675 bp average fragment length
   5% of sequence in fragments shorter than     966 bp
  10% of sequence in fragments shorter than    1444 bp
  15% of sequence in fragments shorter than    1874 bp
  20% of sequence in fragments shorter than    2258 bp
  25% of sequence in fragments shorter than    2648 bp
  30% of sequence in fragments shorter than    3028 bp
  35% of sequence in fragments shorter than    3401 bp
  40% of sequence in fragments shorter than    3800 bp
  45% of sequence in fragments shorter than    4221 bp
  50% of sequence in fragments shorter than    4651 bp
  55% of sequence in fragments shorter than    5128 bp
  60% of sequence in fragments shorter than    5645 bp
  65% of sequence in fragments shorter than    6216 bp
  70% of sequence in fragments shorter than    6863 bp
  75% of sequence in fragments shorter than    7607 bp
  80% of sequence in fragments shorter than    8512 bp
  85% of sequence in fragments shorter than    9653 bp
  90% of sequence in fragments shorter than   11290 bp
  95% of sequence in fragments shorter than   14191 bp

# FnuDII.SapI (CGCG+GCTCTTC) double digest, 1110 thousand fragments (733940+377161 sites), 2682 bp average fragment length
   5% of sequence in fragments shorter than    1060 bp
  10% of sequence in fragments shorter than    1777 bp
  15% of sequence in fragments shorter than    2421 bp
  20% of sequence in fragments shorter than    3036 bp
  25% of sequence in fragments shorter than    3652 bp
  30% of sequence in fragments shorter than    4281 bp
  35% of sequence in fragments shorter than    4926 bp
  40% of sequence in fragments shorter than    5601 bp
  45% of sequence in fragments shorter than    6325 bp
  50% of sequence in fragments shorter than    7104 bp
  55% of sequence in fragments shorter than    7946 bp
  60% of sequence in fragments shorter than    8887 bp
  65% of sequence in fragments shorter than    9934 bp
  70% of sequence in fragments shorter than   11140 bp
  75% of sequence in fragments shorter than   12580 bp
  80% of sequence in fragments shorter than   14344 bp
  85% of sequence in fragments shorter than   16643 bp
  90% of sequence in fragments shorter than   20077 bp
  95% of sequence in fragments shorter than   26816 bp

# FnuDII.SmaI (CGCG+CCCGGG) double digest, 1110 thousand fragments (733940+376940 sites), 2683 bp average fragment length
   5% of sequence in fragments shorter than    1049 bp
  10% of sequence in fragments shorter than    1903 bp
  15% of sequence in fragments shorter than    2721 bp
  20% of sequence in fragments shorter than    3570 bp
  25% of sequence in fragments shorter than    4484 bp
  30% of sequence in fragments shorter than    5450 bp
  35% of sequence in fragments shorter than    6502 bp
  40% of sequence in fragments shorter than    7651 bp
  45% of sequence in fragments shorter than    8928 bp
  50% of sequence in fragments shorter than   10329 bp
  55% of sequence in fragments shorter than   11902 bp
  60% of sequence in fragments shorter than   13670 bp
  65% of sequence in fragments shorter than   15750 bp
  70% of sequence in fragments shorter than   18173 bp
  75% of sequence in fragments shorter than   21051 bp
  80% of sequence in fragments shorter than   24630 bp
  85% of sequence in fragments shorter than   29428 bp
  90% of sequence in fragments shorter than   36716 bp
  95% of sequence in fragments shorter than   50953 bp

# AccI.NotI (GTMKAC+GCGGCCGC) double digest, 1110 thousand fragments (1101063+9795 sites), 2683 bp average fragment length
   5% of sequence in fragments shorter than     951 bp
  10% of sequence in fragments shorter than    1425 bp
  15% of sequence in fragments shorter than    1848 bp
  20% of sequence in fragments shorter than    2239 bp
  25% of sequence in fragments shorter than    2623 bp
  30% of sequence in fragments shorter than    3004 bp
  35% of sequence in fragments shorter than    3401 bp
  40% of sequence in fragments shorter than    3804 bp
  45% of sequence in fragments shorter than    4233 bp
  50% of sequence in fragments shorter than    4679 bp
  55% of sequence in fragments shorter than    5166 bp
  60% of sequence in fragments shorter than    5670 bp
  65% of sequence in fragments shorter than    6201 bp
  70% of sequence in fragments shorter than    6866 bp
  75% of sequence in fragments shorter than    7627 bp
  80% of sequence in fragments shorter than    8569 bp
  85% of sequence in fragments shorter than    9789 bp
  90% of sequence in fragments shorter than   11526 bp
  95% of sequence in fragments shorter than   14753 bp

# AccI.RsrII (GTMKAC+CGGWCCG) double digest, 1110 thousand fragments (1101063+9764 sites), 2683 bp average fragment length
   5% of sequence in fragments shorter than     951 bp
  10% of sequence in fragments shorter than    1425 bp
  15% of sequence in fragments shorter than    1848 bp
  20% of sequence in fragments shorter than    2239 bp
  25% of sequence in fragments shorter than    2622 bp
  30% of sequence in fragments shorter than    3002 bp
  35% of sequence in fragments shorter than    3399 bp
  40% of sequence in fragments shorter than    3801 bp
  45% of sequence in fragments shorter than    4230 bp
  50% of sequence in fragments shorter than    4677 bp
  55% of sequence in fragments shorter than    5163 bp
  60% of sequence in fragments shorter than    5668 bp
  65% of sequence in fragments shorter than    6199 bp
  70% of sequence in fragments shorter than    6865 bp
  75% of sequence in fragments shorter than    7626 bp
  80% of sequence in fragments shorter than    8566 bp
  85% of sequence in fragments shorter than    9783 bp
  90% of sequence in fragments shorter than   11521 bp
  95% of sequence in fragments shorter than   14754 bp

# BglII.BstEII (AGATCT+GGTNACC) double digest, 1110 thousand fragments (774732+335865 sites), 2684 bp average fragment length
   5% of sequence in fragments shorter than     930 bp
  10% of sequence in fragments shorter than    1403 bp
  15% of sequence in fragments shorter than    1823 bp
  20% of sequence in fragments shorter than    2214 bp
  25% of sequence in fragments shorter than    2598 bp
  30% of sequence in fragments shorter than    2986 bp
  35% of sequence in fragments shorter than    3380 bp
  40% of sequence in fragments shorter than    3785 bp
  45% of sequence in fragments shorter than    4202 bp
  50% of sequence in fragments shorter than    4642 bp
  55% of sequence in fragments shorter than    5113 bp
  60% of sequence in fragments shorter than    5629 bp
  65% of sequence in fragments shorter than    6204 bp
  70% of sequence in fragments shorter than    6858 bp
  75% of sequence in fragments shorter than    7623 bp
  80% of sequence in fragments shorter than    8548 bp
  85% of sequence in fragments shorter than    9770 bp
  90% of sequence in fragments shorter than   11532 bp
  95% of sequence in fragments shorter than   15111 bp

# BspHI.SnaBI (TCATGA+TACGTA) double digest, 1110 thousand fragments (978290+132299 sites), 2684 bp average fragment length
   5% of sequence in fragments shorter than     947 bp
  10% of sequence in fragments shorter than    1430 bp
  15% of sequence in fragments shorter than    1856 bp
  20% of sequence in fragments shorter than    2230 bp
  25% of sequence in fragments shorter than    2608 bp
  30% of sequence in fragments shorter than    2992 bp
  35% of sequence in fragments shorter than    3379 bp
  40% of sequence in fragments shorter than    3787 bp
  45% of sequence in fragments shorter than    4213 bp
  50% of sequence in fragments shorter than    4660 bp
  55% of sequence in fragments shorter than    5146 bp
  60% of sequence in fragments shorter than    5670 bp
  65% of sequence in fragments shorter than    6261 bp
  70% of sequence in fragments shorter than    6931 bp
  75% of sequence in fragments shorter than    7726 bp
  80% of sequence in fragments shorter than    8694 bp
  85% of sequence in fragments shorter than    9992 bp
  90% of sequence in fragments shorter than   11905 bp
  95% of sequence in fragments shorter than   15733 bp

# MfeI.ScaI (CAATTG+AGTACT) double digest, 1107 thousand fragments (564303+543793 sites), 2690 bp average fragment length
   5% of sequence in fragments shorter than     925 bp
  10% of sequence in fragments shorter than    1405 bp
  15% of sequence in fragments shorter than    1826 bp
  20% of sequence in fragments shorter than    2209 bp
  25% of sequence in fragments shorter than    2592 bp
  30% of sequence in fragments shorter than    2977 bp
  35% of sequence in fragments shorter than    3374 bp
  40% of sequence in fragments shorter than    3789 bp
  45% of sequence in fragments shorter than    4225 bp
  50% of sequence in fragments shorter than    4681 bp
  55% of sequence in fragments shorter than    5177 bp
  60% of sequence in fragments shorter than    5723 bp
  65% of sequence in fragments shorter than    6339 bp
  70% of sequence in fragments shorter than    7050 bp
  75% of sequence in fragments shorter than    7890 bp
  80% of sequence in fragments shorter than    8961 bp
  85% of sequence in fragments shorter than   10378 bp
  90% of sequence in fragments shorter than   12550 bp
  95% of sequence in fragments shorter than   17073 bp

# MluI.PpuMI (ACGCGT+RGGWCCY) double digest, 1106 thousand fragments (21415+1085138 sites), 2693 bp average fragment length
   5% of sequence in fragments shorter than     873 bp
  10% of sequence in fragments shorter than    1418 bp
  15% of sequence in fragments shorter than    1917 bp
  20% of sequence in fragments shorter than    2402 bp
  25% of sequence in fragments shorter than    2895 bp
  30% of sequence in fragments shorter than    3388 bp
  35% of sequence in fragments shorter than    3854 bp
  40% of sequence in fragments shorter than    4396 bp
  45% of sequence in fragments shorter than    4983 bp
  50% of sequence in fragments shorter than    5612 bp
  55% of sequence in fragments shorter than    6304 bp
  60% of sequence in fragments shorter than    7072 bp
  65% of sequence in fragments shorter than    7929 bp
  70% of sequence in fragments shorter than    8918 bp
  75% of sequence in fragments shorter than   10093 bp
  80% of sequence in fragments shorter than   11518 bp
  85% of sequence in fragments shorter than   13446 bp
  90% of sequence in fragments shorter than   16288 bp
  95% of sequence in fragments shorter than   22243 bp

# AclI.AvaIII (AACGTT+ATGCAT) double digest, 1105 thousand fragments (172706+933357 sites), 2695 bp average fragment length
   5% of sequence in fragments shorter than     957 bp
  10% of sequence in fragments shorter than    1442 bp
  15% of sequence in fragments shorter than    1878 bp
  20% of sequence in fragments shorter than    2268 bp
  25% of sequence in fragments shorter than    2665 bp
  30% of sequence in fragments shorter than    3064 bp
  35% of sequence in fragments shorter than    3462 bp
  40% of sequence in fragments shorter than    3892 bp
  45% of sequence in fragments shorter than    4344 bp
  50% of sequence in fragments shorter than    4826 bp
  55% of sequence in fragments shorter than    5341 bp
  60% of sequence in fragments shorter than    5910 bp
  65% of sequence in fragments shorter than    6541 bp
  70% of sequence in fragments shorter than    7269 bp
  75% of sequence in fragments shorter than    8114 bp
  80% of sequence in fragments shorter than    9163 bp
  85% of sequence in fragments shorter than   10562 bp
  90% of sequence in fragments shorter than   12586 bp
  95% of sequence in fragments shorter than   16534 bp

# AccI.AscI (GTMKAC+GGCGCGCC) double digest, 1105 thousand fragments (1101063+4751 sites), 2695 bp average fragment length
   5% of sequence in fragments shorter than     955 bp
  10% of sequence in fragments shorter than    1431 bp
  15% of sequence in fragments shorter than    1856 bp
  20% of sequence in fragments shorter than    2247 bp
  25% of sequence in fragments shorter than    2634 bp
  30% of sequence in fragments shorter than    3016 bp
  35% of sequence in fragments shorter than    3416 bp
  40% of sequence in fragments shorter than    3819 bp
  45% of sequence in fragments shorter than    4251 bp
  50% of sequence in fragments shorter than    4700 bp
  55% of sequence in fragments shorter than    5189 bp
  60% of sequence in fragments shorter than    5698 bp
  65% of sequence in fragments shorter than    6235 bp
  70% of sequence in fragments shorter than    6907 bp
  75% of sequence in fragments shorter than    7677 bp
  80% of sequence in fragments shorter than    8627 bp
  85% of sequence in fragments shorter than    9852 bp
  90% of sequence in fragments shorter than   11622 bp
  95% of sequence in fragments shorter than   14922 bp

# MluI.PvuII (ACGCGT+CAGCTG) double digest, 1105 thousand fragments (21415+1084260 sites), 2696 bp average fragment length
   5% of sequence in fragments shorter than     902 bp
  10% of sequence in fragments shorter than    1407 bp
  15% of sequence in fragments shorter than    1869 bp
  20% of sequence in fragments shorter than    2315 bp
  25% of sequence in fragments shorter than    2753 bp
  30% of sequence in fragments shorter than    3193 bp
  35% of sequence in fragments shorter than    3658 bp
  40% of sequence in fragments shorter than    4154 bp
  45% of sequence in fragments shorter than    4677 bp
  50% of sequence in fragments shorter than    5245 bp
  55% of sequence in fragments shorter than    5856 bp
  60% of sequence in fragments shorter than    6523 bp
  65% of sequence in fragments shorter than    7258 bp
  70% of sequence in fragments shorter than    8116 bp
  75% of sequence in fragments shorter than    9114 bp
  80% of sequence in fragments shorter than   10381 bp
  85% of sequence in fragments shorter than   11998 bp
  90% of sequence in fragments shorter than   14471 bp
  95% of sequence in fragments shorter than   19276 bp

# BceAI.SpeI (ACGGC+ACTAGT) double digest, 1105 thousand fragments (705178+400286 sites), 2696 bp average fragment length
   5% of sequence in fragments shorter than     930 bp
  10% of sequence in fragments shorter than    1443 bp
  15% of sequence in fragments shorter than    1899 bp
  20% of sequence in fragments shorter than    2328 bp
  25% of sequence in fragments shorter than    2749 bp
  30% of sequence in fragments shorter than    3177 bp
  35% of sequence in fragments shorter than    3613 bp
  40% of sequence in fragments shorter than    4067 bp
  45% of sequence in fragments shorter than    4537 bp
  50% of sequence in fragments shorter than    5002 bp
  55% of sequence in fragments shorter than    5521 bp
  60% of sequence in fragments shorter than    6108 bp
  65% of sequence in fragments shorter than    6754 bp
  70% of sequence in fragments shorter than    7492 bp
  75% of sequence in fragments shorter than    8355 bp
  80% of sequence in fragments shorter than    9394 bp
  85% of sequence in fragments shorter than   10739 bp
  90% of sequence in fragments shorter than   12667 bp
  95% of sequence in fragments shorter than   16148 bp

# BamHI.BclI (GGATCC+TGATCA) double digest, 1104 thousand fragments (365999+738785 sites), 2698 bp average fragment length
   5% of sequence in fragments shorter than     958 bp
  10% of sequence in fragments shorter than    1419 bp
  15% of sequence in fragments shorter than    1835 bp
  20% of sequence in fragments shorter than    2218 bp
  25% of sequence in fragments shorter than    2593 bp
  30% of sequence in fragments shorter than    2965 bp
  35% of sequence in fragments shorter than    3347 bp
  40% of sequence in fragments shorter than    3740 bp
  45% of sequence in fragments shorter than    4154 bp
  50% of sequence in fragments shorter than    4587 bp
  55% of sequence in fragments shorter than    5048 bp
  60% of sequence in fragments shorter than    5553 bp
  65% of sequence in fragments shorter than    6113 bp
  70% of sequence in fragments shorter than    6753 bp
  75% of sequence in fragments shorter than    7490 bp
  80% of sequence in fragments shorter than    8382 bp
  85% of sequence in fragments shorter than    9523 bp
  90% of sequence in fragments shorter than   11222 bp
  95% of sequence in fragments shorter than   14473 bp

# BspHI.NaeI (TCATGA+GCCGGC) double digest, 1103 thousand fragments (978290+125692 sites), 2700 bp average fragment length
   5% of sequence in fragments shorter than     960 bp
  10% of sequence in fragments shorter than    1463 bp
  15% of sequence in fragments shorter than    1893 bp
  20% of sequence in fragments shorter than    2271 bp
  25% of sequence in fragments shorter than    2651 bp
  30% of sequence in fragments shorter than    3035 bp
  35% of sequence in fragments shorter than    3421 bp
  40% of sequence in fragments shorter than    3825 bp
  45% of sequence in fragments shorter than    4244 bp
  50% of sequence in fragments shorter than    4685 bp
  55% of sequence in fragments shorter than    5158 bp
  60% of sequence in fragments shorter than    5669 bp
  65% of sequence in fragments shorter than    6236 bp
  70% of sequence in fragments shorter than    6874 bp
  75% of sequence in fragments shorter than    7627 bp
  80% of sequence in fragments shorter than    8539 bp
  85% of sequence in fragments shorter than    9720 bp
  90% of sequence in fragments shorter than   11456 bp
  95% of sequence in fragments shorter than   14791 bp

# AflII.ApaI (CTTAAG+GGGCCC) double digest, 1103 thousand fragments (643284+460339 sites), 2701 bp average fragment length
   5% of sequence in fragments shorter than     924 bp
  10% of sequence in fragments shorter than    1434 bp
  15% of sequence in fragments shorter than    1885 bp
  20% of sequence in fragments shorter than    2307 bp
  25% of sequence in fragments shorter than    2720 bp
  30% of sequence in fragments shorter than    3136 bp
  35% of sequence in fragments shorter than    3565 bp
  40% of sequence in fragments shorter than    3998 bp
  45% of sequence in fragments shorter than    4456 bp
  50% of sequence in fragments shorter than    4942 bp
  55% of sequence in fragments shorter than    5473 bp
  60% of sequence in fragments shorter than    6047 bp
  65% of sequence in fragments shorter than    6687 bp
  70% of sequence in fragments shorter than    7420 bp
  75% of sequence in fragments shorter than    8273 bp
  80% of sequence in fragments shorter than    9306 bp
  85% of sequence in fragments shorter than   10666 bp
  90% of sequence in fragments shorter than   12674 bp
  95% of sequence in fragments shorter than   16816 bp

# BsaAI.HpaI (YACGTR+GTTAAC) double digest, 1102 thousand fragments (713801+389418 sites), 2702 bp average fragment length
   5% of sequence in fragments shorter than     968 bp
  10% of sequence in fragments shorter than    1444 bp
  15% of sequence in fragments shorter than    1875 bp
  20% of sequence in fragments shorter than    2269 bp
  25% of sequence in fragments shorter than    2664 bp
  30% of sequence in fragments shorter than    3048 bp
  35% of sequence in fragments shorter than    3431 bp
  40% of sequence in fragments shorter than    3821 bp
  45% of sequence in fragments shorter than    4246 bp
  50% of sequence in fragments shorter than    4692 bp
  55% of sequence in fragments shorter than    5177 bp
  60% of sequence in fragments shorter than    5709 bp
  65% of sequence in fragments shorter than    6298 bp
  70% of sequence in fragments shorter than    6951 bp
  75% of sequence in fragments shorter than    7710 bp
  80% of sequence in fragments shorter than    8640 bp
  85% of sequence in fragments shorter than    9825 bp
  90% of sequence in fragments shorter than   11533 bp
  95% of sequence in fragments shorter than   14636 bp

# AccI.SalI (GTMKAC+GTCGAC) double digest, 1100 thousand fragments (1101063+31396 sites), 2707 bp average fragment length
   5% of sequence in fragments shorter than     958 bp
  10% of sequence in fragments shorter than    1437 bp
  15% of sequence in fragments shorter than    1863 bp
  20% of sequence in fragments shorter than    2257 bp
  25% of sequence in fragments shorter than    2645 bp
  30% of sequence in fragments shorter than    3030 bp
  35% of sequence in fragments shorter than    3431 bp
  40% of sequence in fragments shorter than    3837 bp
  45% of sequence in fragments shorter than    4272 bp
  50% of sequence in fragments shorter than    4724 bp
  55% of sequence in fragments shorter than    5216 bp
  60% of sequence in fragments shorter than    5729 bp
  65% of sequence in fragments shorter than    6269 bp
  70% of sequence in fragments shorter than    6946 bp
  75% of sequence in fragments shorter than    7724 bp
  80% of sequence in fragments shorter than    8680 bp
  85% of sequence in fragments shorter than    9926 bp
  90% of sequence in fragments shorter than   11712 bp
  95% of sequence in fragments shorter than   15063 bp

# PpuMI.SgrAI (RGGWCCY+CRCCGGYG) double digest, 1100 thousand fragments (1085138+15270 sites), 2708 bp average fragment length
   5% of sequence in fragments shorter than     879 bp
  10% of sequence in fragments shorter than    1429 bp
  15% of sequence in fragments shorter than    1933 bp
  20% of sequence in fragments shorter than    2425 bp
  25% of sequence in fragments shorter than    2923 bp
  30% of sequence in fragments shorter than    3421 bp
  35% of sequence in fragments shorter than    3891 bp
  40% of sequence in fragments shorter than    4440 bp
  45% of sequence in fragments shorter than    5033 bp
  50% of sequence in fragments shorter than    5675 bp
  55% of sequence in fragments shorter than    6369 bp
  60% of sequence in fragments shorter than    7147 bp
  65% of sequence in fragments shorter than    8015 bp
  70% of sequence in fragments shorter than    9008 bp
  75% of sequence in fragments shorter than   10201 bp
  80% of sequence in fragments shorter than   11656 bp
  85% of sequence in fragments shorter than   13583 bp
  90% of sequence in fragments shorter than   16494 bp
  95% of sequence in fragments shorter than   22547 bp

# NruI.PpuMI (TCGCGA+RGGWCCY) double digest, 1099 thousand fragments (14872+1085138 sites), 2709 bp average fragment length
   5% of sequence in fragments shorter than     880 bp
  10% of sequence in fragments shorter than    1427 bp
  15% of sequence in fragments shorter than    1928 bp
  20% of sequence in fragments shorter than    2416 bp
  25% of sequence in fragments shorter than    2910 bp
  30% of sequence in fragments shorter than    3403 bp
  35% of sequence in fragments shorter than    3871 bp
  40% of sequence in fragments shorter than    4417 bp
  45% of sequence in fragments shorter than    5002 bp
  50% of sequence in fragments shorter than    5637 bp
  55% of sequence in fragments shorter than    6327 bp
  60% of sequence in fragments shorter than    7097 bp
  65% of sequence in fragments shorter than    7955 bp
  70% of sequence in fragments shorter than    8948 bp
  75% of sequence in fragments shorter than   10128 bp
  80% of sequence in fragments shorter than   11563 bp
  85% of sequence in fragments shorter than   13487 bp
  90% of sequence in fragments shorter than   16363 bp
  95% of sequence in fragments shorter than   22388 bp

# BamHI.FnuDII (GGATCC+CGCG) double digest, 1099 thousand fragments (365999+733940 sites), 2710 bp average fragment length
   5% of sequence in fragments shorter than    1083 bp
  10% of sequence in fragments shorter than    1819 bp
  15% of sequence in fragments shorter than    2462 bp
  20% of sequence in fragments shorter than    3103 bp
  25% of sequence in fragments shorter than    3740 bp
  30% of sequence in fragments shorter than    4394 bp
  35% of sequence in fragments shorter than    5046 bp
  40% of sequence in fragments shorter than    5761 bp
  45% of sequence in fragments shorter than    6530 bp
  50% of sequence in fragments shorter than    7364 bp
  55% of sequence in fragments shorter than    8288 bp
  60% of sequence in fragments shorter than    9290 bp
  65% of sequence in fragments shorter than   10433 bp
  70% of sequence in fragments shorter than   11710 bp
  75% of sequence in fragments shorter than   13254 bp
  80% of sequence in fragments shorter than   15185 bp
  85% of sequence in fragments shorter than   17680 bp
  90% of sequence in fragments shorter than   21367 bp
  95% of sequence in fragments shorter than   28495 bp

# BspHI.XhoI (TCATGA+CTCGAG) double digest, 1099 thousand fragments (978290+121308 sites), 2711 bp average fragment length
   5% of sequence in fragments shorter than     961 bp
  10% of sequence in fragments shorter than    1458 bp
  15% of sequence in fragments shorter than    1885 bp
  20% of sequence in fragments shorter than    2257 bp
  25% of sequence in fragments shorter than    2634 bp
  30% of sequence in fragments shorter than    2988 bp
  35% of sequence in fragments shorter than    3368 bp
  40% of sequence in fragments shorter than    3769 bp
  45% of sequence in fragments shorter than    4184 bp
  50% of sequence in fragments shorter than    4625 bp
  55% of sequence in fragments shorter than    5100 bp
  60% of sequence in fragments shorter than    5613 bp
  65% of sequence in fragments shorter than    6187 bp
  70% of sequence in fragments shorter than    6830 bp
  75% of sequence in fragments shorter than    7586 bp
  80% of sequence in fragments shorter than    8505 bp
  85% of sequence in fragments shorter than    9710 bp
  90% of sequence in fragments shorter than   11441 bp
  95% of sequence in fragments shorter than   14835 bp

# PvuII.SgrAI (CAGCTG+CRCCGGYG) double digest, 1099 thousand fragments (1084260+15270 sites), 2711 bp average fragment length
   5% of sequence in fragments shorter than     907 bp
  10% of sequence in fragments shorter than    1417 bp
  15% of sequence in fragments shorter than    1883 bp
  20% of sequence in fragments shorter than    2333 bp
  25% of sequence in fragments shorter than    2770 bp
  30% of sequence in fragments shorter than    3219 bp
  35% of sequence in fragments shorter than    3687 bp
  40% of sequence in fragments shorter than    4187 bp
  45% of sequence in fragments shorter than    4714 bp
  50% of sequence in fragments shorter than    5289 bp
  55% of sequence in fragments shorter than    5904 bp
  60% of sequence in fragments shorter than    6572 bp
  65% of sequence in fragments shorter than    7315 bp
  70% of sequence in fragments shorter than    8181 bp
  75% of sequence in fragments shorter than    9194 bp
  80% of sequence in fragments shorter than   10475 bp
  85% of sequence in fragments shorter than   12111 bp
  90% of sequence in fragments shorter than   14600 bp
  95% of sequence in fragments shorter than   19442 bp

# ApaLI.SacI (GTGCAC+GAGCTC) double digest, 1099 thousand fragments (496255+603323 sites), 2711 bp average fragment length
   5% of sequence in fragments shorter than     939 bp
  10% of sequence in fragments shorter than    1435 bp
  15% of sequence in fragments shorter than    1865 bp
  20% of sequence in fragments shorter than    2276 bp
  25% of sequence in fragments shorter than    2689 bp
  30% of sequence in fragments shorter than    3104 bp
  35% of sequence in fragments shorter than    3525 bp
  40% of sequence in fragments shorter than    3966 bp
  45% of sequence in fragments shorter than    4368 bp
  50% of sequence in fragments shorter than    4855 bp
  55% of sequence in fragments shorter than    5387 bp
  60% of sequence in fragments shorter than    5962 bp
  65% of sequence in fragments shorter than    6617 bp
  70% of sequence in fragments shorter than    7369 bp
  75% of sequence in fragments shorter than    8247 bp
  80% of sequence in fragments shorter than    9323 bp
  85% of sequence in fragments shorter than   10737 bp
  90% of sequence in fragments shorter than   12787 bp
  95% of sequence in fragments shorter than   16715 bp

# PpuMI.PvuI (RGGWCCY+CGATCG) double digest, 1098 thousand fragments (1085138+14186 sites), 2711 bp average fragment length
   5% of sequence in fragments shorter than     882 bp
  10% of sequence in fragments shorter than    1430 bp
  15% of sequence in fragments shorter than    1924 bp
  20% of sequence in fragments shorter than    2414 bp
  25% of sequence in fragments shorter than    2910 bp
  30% of sequence in fragments shorter than    3403 bp
  35% of sequence in fragments shorter than    3871 bp
  40% of sequence in fragments shorter than    4416 bp
  45% of sequence in fragments shorter than    5003 bp
  50% of sequence in fragments shorter than    5637 bp
  55% of sequence in fragments shorter than    6329 bp
  60% of sequence in fragments shorter than    7100 bp
  65% of sequence in fragments shorter than    7961 bp
  70% of sequence in fragments shorter than    8949 bp
  75% of sequence in fragments shorter than   10129 bp
  80% of sequence in fragments shorter than   11565 bp
  85% of sequence in fragments shorter than   13480 bp
  90% of sequence in fragments shorter than   16319 bp
  95% of sequence in fragments shorter than   22114 bp

# NruI.PvuII (TCGCGA+CAGCTG) double digest, 1098 thousand fragments (14872+1084260 sites), 2712 bp average fragment length
   5% of sequence in fragments shorter than     907 bp
  10% of sequence in fragments shorter than    1416 bp
  15% of sequence in fragments shorter than    1880 bp
  20% of sequence in fragments shorter than    2328 bp
  25% of sequence in fragments shorter than    2763 bp
  30% of sequence in fragments shorter than    3209 bp
  35% of sequence in fragments shorter than    3675 bp
  40% of sequence in fragments shorter than    4172 bp
  45% of sequence in fragments shorter than    4694 bp
  50% of sequence in fragments shorter than    5263 bp
  55% of sequence in fragments shorter than    5876 bp
  60% of sequence in fragments shorter than    6543 bp
  65% of sequence in fragments shorter than    7280 bp
  70% of sequence in fragments shorter than    8144 bp
  75% of sequence in fragments shorter than    9145 bp
  80% of sequence in fragments shorter than   10414 bp
  85% of sequence in fragments shorter than   12039 bp
  90% of sequence in fragments shorter than   14514 bp
  95% of sequence in fragments shorter than   19341 bp

# FseI.PpuMI (GGCCGGCC+RGGWCCY) double digest, 1098 thousand fragments (13662+1085138 sites), 2712 bp average fragment length
   5% of sequence in fragments shorter than     880 bp
  10% of sequence in fragments shorter than    1431 bp
  15% of sequence in fragments shorter than    1935 bp
  20% of sequence in fragments shorter than    2427 bp
  25% of sequence in fragments shorter than    2926 bp
  30% of sequence in fragments shorter than    3424 bp
  35% of sequence in fragments shorter than    3895 bp
  40% of sequence in fragments shorter than    4446 bp
  45% of sequence in fragments shorter than    5037 bp
  50% of sequence in fragments shorter than    5680 bp
  55% of sequence in fragments shorter than    6376 bp
  60% of sequence in fragments shorter than    7153 bp
  65% of sequence in fragments shorter than    8025 bp
  70% of sequence in fragments shorter than    9017 bp
  75% of sequence in fragments shorter than   10216 bp
  80% of sequence in fragments shorter than   11671 bp
  85% of sequence in fragments shorter than   13609 bp
  90% of sequence in fragments shorter than   16519 bp
  95% of sequence in fragments shorter than   22577 bp

# PvuI.PvuII (CGATCG+CAGCTG) double digest, 1098 thousand fragments (14186+1084260 sites), 2713 bp average fragment length
   5% of sequence in fragments shorter than     910 bp
  10% of sequence in fragments shorter than    1418 bp
  15% of sequence in fragments shorter than    1882 bp
  20% of sequence in fragments shorter than    2326 bp
  25% of sequence in fragments shorter than    2761 bp
  30% of sequence in fragments shorter than    3208 bp
  35% of sequence in fragments shorter than    3675 bp
  40% of sequence in fragments shorter than    4174 bp
  45% of sequence in fragments shorter than    4697 bp
  50% of sequence in fragments shorter than    5268 bp
  55% of sequence in fragments shorter than    5878 bp
  60% of sequence in fragments shorter than    6546 bp
  65% of sequence in fragments shorter than    7281 bp
  70% of sequence in fragments shorter than    8141 bp
  75% of sequence in fragments shorter than    9138 bp
  80% of sequence in fragments shorter than   10402 bp
  85% of sequence in fragments shorter than   12026 bp
  90% of sequence in fragments shorter than   14468 bp
  95% of sequence in fragments shorter than   19161 bp

# AclI.StuI (AACGTT+AGGCCT) double digest, 1097 thousand fragments (172706+925729 sites), 2714 bp average fragment length
   5% of sequence in fragments shorter than     843 bp
  10% of sequence in fragments shorter than    1393 bp
  15% of sequence in fragments shorter than    1912 bp
  20% of sequence in fragments shorter than    2377 bp
  25% of sequence in fragments shorter than    2863 bp
  30% of sequence in fragments shorter than    3332 bp
  35% of sequence in fragments shorter than    3820 bp
  40% of sequence in fragments shorter than    4328 bp
  45% of sequence in fragments shorter than    4859 bp
  50% of sequence in fragments shorter than    5424 bp
  55% of sequence in fragments shorter than    6033 bp
  60% of sequence in fragments shorter than    6700 bp
  65% of sequence in fragments shorter than    7438 bp
  70% of sequence in fragments shorter than    8257 bp
  75% of sequence in fragments shorter than    9215 bp
  80% of sequence in fragments shorter than   10356 bp
  85% of sequence in fragments shorter than   11805 bp
  90% of sequence in fragments shorter than   13797 bp
  95% of sequence in fragments shorter than   17212 bp

# FseI.PvuII (GGCCGGCC+CAGCTG) double digest, 1097 thousand fragments (13662+1084260 sites), 2715 bp average fragment length
   5% of sequence in fragments shorter than     908 bp
  10% of sequence in fragments shorter than    1419 bp
  15% of sequence in fragments shorter than    1886 bp
  20% of sequence in fragments shorter than    2336 bp
  25% of sequence in fragments shorter than    2774 bp
  30% of sequence in fragments shorter than    3224 bp
  35% of sequence in fragments shorter than    3693 bp
  40% of sequence in fragments shorter than    4196 bp
  45% of sequence in fragments shorter than    4724 bp
  50% of sequence in fragments shorter than    5301 bp
  55% of sequence in fragments shorter than    5915 bp
  60% of sequence in fragments shorter than    6587 bp
  65% of sequence in fragments shorter than    7329 bp
  70% of sequence in fragments shorter than    8196 bp
  75% of sequence in fragments shorter than    9209 bp
  80% of sequence in fragments shorter than   10490 bp
  85% of sequence in fragments shorter than   12131 bp
  90% of sequence in fragments shorter than   14627 bp
  95% of sequence in fragments shorter than   19492 bp

# BciVI.SpeI (GTATCC+ACTAGT) double digest, 1095 thousand fragments (696093+400286 sites), 2719 bp average fragment length
   5% of sequence in fragments shorter than     966 bp
  10% of sequence in fragments shorter than    1414 bp
  15% of sequence in fragments shorter than    1834 bp
  20% of sequence in fragments shorter than    2219 bp
  25% of sequence in fragments shorter than    2608 bp
  30% of sequence in fragments shorter than    3005 bp
  35% of sequence in fragments shorter than    3404 bp
  40% of sequence in fragments shorter than    3817 bp
  45% of sequence in fragments shorter than    4242 bp
  50% of sequence in fragments shorter than    4703 bp
  55% of sequence in fragments shorter than    5190 bp
  60% of sequence in fragments shorter than    5730 bp
  65% of sequence in fragments shorter than    6330 bp
  70% of sequence in fragments shorter than    7010 bp
  75% of sequence in fragments shorter than    7812 bp
  80% of sequence in fragments shorter than    8799 bp
  85% of sequence in fragments shorter than   10110 bp
  90% of sequence in fragments shorter than   12036 bp
  95% of sequence in fragments shorter than   15750 bp

# BsrBI.HindIII (CCGCTC+AAGCTT) double digest, 1095 thousand fragments (235523+860365 sites), 2720 bp average fragment length
   5% of sequence in fragments shorter than     948 bp
  10% of sequence in fragments shorter than    1437 bp
  15% of sequence in fragments shorter than    1891 bp
  20% of sequence in fragments shorter than    2281 bp
  25% of sequence in fragments shorter than    2685 bp
  30% of sequence in fragments shorter than    3083 bp
  35% of sequence in fragments shorter than    3490 bp
  40% of sequence in fragments shorter than    3910 bp
  45% of sequence in fragments shorter than    4347 bp
  50% of sequence in fragments shorter than    4806 bp
  55% of sequence in fragments shorter than    5296 bp
  60% of sequence in fragments shorter than    5834 bp
  65% of sequence in fragments shorter than    6432 bp
  70% of sequence in fragments shorter than    7100 bp
  75% of sequence in fragments shorter than    7882 bp
  80% of sequence in fragments shorter than    8825 bp
  85% of sequence in fragments shorter than   10027 bp
  90% of sequence in fragments shorter than   11737 bp
  95% of sequence in fragments shorter than   14816 bp

# BstEII.NcoI (GGTNACC+CCATGG) double digest, 1095 thousand fragments (335865+759594 sites), 2721 bp average fragment length
   5% of sequence in fragments shorter than     938 bp
  10% of sequence in fragments shorter than    1437 bp
  15% of sequence in fragments shorter than    1884 bp
  20% of sequence in fragments shorter than    2309 bp
  25% of sequence in fragments shorter than    2728 bp
  30% of sequence in fragments shorter than    3144 bp
  35% of sequence in fragments shorter than    3571 bp
  40% of sequence in fragments shorter than    4016 bp
  45% of sequence in fragments shorter than    4418 bp
  50% of sequence in fragments shorter than    4908 bp
  55% of sequence in fragments shorter than    5440 bp
  60% of sequence in fragments shorter than    6028 bp
  65% of sequence in fragments shorter than    6679 bp
  70% of sequence in fragments shorter than    7443 bp
  75% of sequence in fragments shorter than    8337 bp
  80% of sequence in fragments shorter than    9428 bp
  85% of sequence in fragments shorter than   10856 bp
  90% of sequence in fragments shorter than   12953 bp
  95% of sequence in fragments shorter than   17079 bp

# NotI.PpuMI (GCGGCCGC+RGGWCCY) double digest, 1094 thousand fragments (9795+1085138 sites), 2722 bp average fragment length
   5% of sequence in fragments shorter than     883 bp
  10% of sequence in fragments shorter than    1436 bp
  15% of sequence in fragments shorter than    1943 bp
  20% of sequence in fragments shorter than    2436 bp
  25% of sequence in fragments shorter than    2939 bp
  30% of sequence in fragments shorter than    3437 bp
  35% of sequence in fragments shorter than    3907 bp
  40% of sequence in fragments shorter than    4460 bp
  45% of sequence in fragments shorter than    5054 bp
  50% of sequence in fragments shorter than    5698 bp
  55% of sequence in fragments shorter than    6396 bp
  60% of sequence in fragments shorter than    7175 bp
  65% of sequence in fragments shorter than    8047 bp
  70% of sequence in fragments shorter than    9040 bp
  75% of sequence in fragments shorter than   10245 bp
  80% of sequence in fragments shorter than   11702 bp
  85% of sequence in fragments shorter than   13648 bp
  90% of sequence in fragments shorter than   16564 bp
  95% of sequence in fragments shorter than   22617 bp

# PpuMI.RsrII (RGGWCCY+CGGWCCG) double digest, 1094 thousand fragments (1085138+9764 sites), 2722 bp average fragment length
   5% of sequence in fragments shorter than     883 bp
  10% of sequence in fragments shorter than    1434 bp
  15% of sequence in fragments shorter than    1940 bp
  20% of sequence in fragments shorter than    2433 bp
  25% of sequence in fragments shorter than    2934 bp
  30% of sequence in fragments shorter than    3432 bp
  35% of sequence in fragments shorter than    3902 bp
  40% of sequence in fragments shorter than    4455 bp
  45% of sequence in fragments shorter than    5047 bp
  50% of sequence in fragments shorter than    5690 bp
  55% of sequence in fragments shorter than    6387 bp
  60% of sequence in fragments shorter than    7166 bp
  65% of sequence in fragments shorter than    8038 bp
  70% of sequence in fragments shorter than    9030 bp
  75% of sequence in fragments shorter than   10232 bp
  80% of sequence in fragments shorter than   11688 bp
  85% of sequence in fragments shorter than   13628 bp
  90% of sequence in fragments shorter than   16541 bp
  95% of sequence in fragments shorter than   22592 bp

# ScaI.SphI (AGTACT+GCATGC) double digest, 1094 thousand fragments (543793+550952 sites), 2723 bp average fragment length
   5% of sequence in fragments shorter than     957 bp
  10% of sequence in fragments shorter than    1440 bp
  15% of sequence in fragments shorter than    1858 bp
  20% of sequence in fragments shorter than    2248 bp
  25% of sequence in fragments shorter than    2631 bp
  30% of sequence in fragments shorter than    3014 bp
  35% of sequence in fragments shorter than    3408 bp
  40% of sequence in fragments shorter than    3815 bp
  45% of sequence in fragments shorter than    4238 bp
  50% of sequence in fragments shorter than    4692 bp
  55% of sequence in fragments shorter than    5187 bp
  60% of sequence in fragments shorter than    5722 bp
  65% of sequence in fragments shorter than    6317 bp
  70% of sequence in fragments shorter than    6988 bp
  75% of sequence in fragments shorter than    7765 bp
  80% of sequence in fragments shorter than    8716 bp
  85% of sequence in fragments shorter than    9938 bp
  90% of sequence in fragments shorter than   11741 bp
  95% of sequence in fragments shorter than   15255 bp

# BceAI.HpaI (ACGGC+GTTAAC) double digest, 1094 thousand fragments (705178+389418 sites), 2723 bp average fragment length
   5% of sequence in fragments shorter than     929 bp
  10% of sequence in fragments shorter than    1453 bp
  15% of sequence in fragments shorter than    1916 bp
  20% of sequence in fragments shorter than    2355 bp
  25% of sequence in fragments shorter than    2795 bp
  30% of sequence in fragments shorter than    3237 bp
  35% of sequence in fragments shorter than    3688 bp
  40% of sequence in fragments shorter than    4156 bp
  45% of sequence in fragments shorter than    4650 bp
  50% of sequence in fragments shorter than    5179 bp
  55% of sequence in fragments shorter than    5741 bp
  60% of sequence in fragments shorter than    6349 bp
  65% of sequence in fragments shorter than    7028 bp
  70% of sequence in fragments shorter than    7810 bp
  75% of sequence in fragments shorter than    8735 bp
  80% of sequence in fragments shorter than    9852 bp
  85% of sequence in fragments shorter than   11305 bp
  90% of sequence in fragments shorter than   13366 bp
  95% of sequence in fragments shorter than   17214 bp

# NotI.PvuII (GCGGCCGC+CAGCTG) double digest, 1093 thousand fragments (9795+1084260 sites), 2724 bp average fragment length
   5% of sequence in fragments shorter than     911 bp
  10% of sequence in fragments shorter than    1423 bp
  15% of sequence in fragments shorter than    1891 bp
  20% of sequence in fragments shorter than    2342 bp
  25% of sequence in fragments shorter than    2782 bp
  30% of sequence in fragments shorter than    3232 bp
  35% of sequence in fragments shorter than    3701 bp
  40% of sequence in fragments shorter than    4206 bp
  45% of sequence in fragments shorter than    4733 bp
  50% of sequence in fragments shorter than    5311 bp
  55% of sequence in fragments shorter than    5927 bp
  60% of sequence in fragments shorter than    6599 bp
  65% of sequence in fragments shorter than    7340 bp
  70% of sequence in fragments shorter than    8207 bp
  75% of sequence in fragments shorter than    9223 bp
  80% of sequence in fragments shorter than   10506 bp
  85% of sequence in fragments shorter than   12148 bp
  90% of sequence in fragments shorter than   14654 bp
  95% of sequence in fragments shorter than   19518 bp

# PvuII.RsrII (CAGCTG+CGGWCCG) double digest, 1093 thousand fragments (1084260+9764 sites), 2724 bp average fragment length
   5% of sequence in fragments shorter than     911 bp
  10% of sequence in fragments shorter than    1423 bp
  15% of sequence in fragments shorter than    1890 bp
  20% of sequence in fragments shorter than    2341 bp
  25% of sequence in fragments shorter than    2779 bp
  30% of sequence in fragments shorter than    3229 bp
  35% of sequence in fragments shorter than    3699 bp
  40% of sequence in fragments shorter than    4201 bp
  45% of sequence in fragments shorter than    4728 bp
  50% of sequence in fragments shorter than    5306 bp
  55% of sequence in fragments shorter than    5920 bp
  60% of sequence in fragments shorter than    6591 bp
  65% of sequence in fragments shorter than    7334 bp
  70% of sequence in fragments shorter than    8199 bp
  75% of sequence in fragments shorter than    9213 bp
  80% of sequence in fragments shorter than   10493 bp
  85% of sequence in fragments shorter than   12130 bp
  90% of sequence in fragments shorter than   14625 bp
  95% of sequence in fragments shorter than   19494 bp

# AvaIII.PacI (ATGCAT+TTAATTAA) double digest, 1092 thousand fragments (933357+159455 sites), 2727 bp average fragment length
   5% of sequence in fragments shorter than     963 bp
  10% of sequence in fragments shorter than    1474 bp
  15% of sequence in fragments shorter than    1918 bp
  20% of sequence in fragments shorter than    2331 bp
  25% of sequence in fragments shorter than    2742 bp
  30% of sequence in fragments shorter than    3158 bp
  35% of sequence in fragments shorter than    3582 bp
  40% of sequence in fragments shorter than    4031 bp
  45% of sequence in fragments shorter than    4506 bp
  50% of sequence in fragments shorter than    5009 bp
  55% of sequence in fragments shorter than    5567 bp
  60% of sequence in fragments shorter than    6177 bp
  65% of sequence in fragments shorter than    6866 bp
  70% of sequence in fragments shorter than    7651 bp
  75% of sequence in fragments shorter than    8588 bp
  80% of sequence in fragments shorter than    9750 bp
  85% of sequence in fragments shorter than   11305 bp
  90% of sequence in fragments shorter than   13665 bp
  95% of sequence in fragments shorter than   18631 bp

# AvaI.ClaI (CYCGRG+ATCGAT) double digest, 1091 thousand fragments (1005714+86477 sites), 2729 bp average fragment length
   5% of sequence in fragments shorter than     885 bp
  10% of sequence in fragments shorter than    1453 bp
  15% of sequence in fragments shorter than    1986 bp
  20% of sequence in fragments shorter than    2517 bp
  25% of sequence in fragments shorter than    3069 bp
  30% of sequence in fragments shorter than    3654 bp
  35% of sequence in fragments shorter than    4267 bp
  40% of sequence in fragments shorter than    4927 bp
  45% of sequence in fragments shorter than    5638 bp
  50% of sequence in fragments shorter than    6420 bp
  55% of sequence in fragments shorter than    7292 bp
  60% of sequence in fragments shorter than    8268 bp
  65% of sequence in fragments shorter than    9390 bp
  70% of sequence in fragments shorter than   10675 bp
  75% of sequence in fragments shorter than   12227 bp
  80% of sequence in fragments shorter than   14172 bp
  85% of sequence in fragments shorter than   16747 bp
  90% of sequence in fragments shorter than   20582 bp
  95% of sequence in fragments shorter than   28381 bp

# AvaIII.Hpy99I (ATGCAT+CGWCG) double digest, 1090 thousand fragments (933357+157917 sites), 2731 bp average fragment length
   5% of sequence in fragments shorter than     977 bp
  10% of sequence in fragments shorter than    1495 bp
  15% of sequence in fragments shorter than    1942 bp
  20% of sequence in fragments shorter than    2350 bp
  25% of sequence in fragments shorter than    2754 bp
  30% of sequence in fragments shorter than    3163 bp
  35% of sequence in fragments shorter than    3578 bp
  40% of sequence in fragments shorter than    4013 bp
  45% of sequence in fragments shorter than    4468 bp
  50% of sequence in fragments shorter than    4943 bp
  55% of sequence in fragments shorter than    5463 bp
  60% of sequence in fragments shorter than    6030 bp
  65% of sequence in fragments shorter than    6650 bp
  70% of sequence in fragments shorter than    7357 bp
  75% of sequence in fragments shorter than    8183 bp
  80% of sequence in fragments shorter than    9182 bp
  85% of sequence in fragments shorter than   10489 bp
  90% of sequence in fragments shorter than   12352 bp
  95% of sequence in fragments shorter than   15865 bp

# ApaLI.AvrII (GTGCAC+CCTAGG) double digest, 1090 thousand fragments (496255+594956 sites), 2731 bp average fragment length
   5% of sequence in fragments shorter than     952 bp
  10% of sequence in fragments shorter than    1427 bp
  15% of sequence in fragments shorter than    1870 bp
  20% of sequence in fragments shorter than    2287 bp
  25% of sequence in fragments shorter than    2694 bp
  30% of sequence in fragments shorter than    3104 bp
  35% of sequence in fragments shorter than    3521 bp
  40% of sequence in fragments shorter than    3950 bp
  45% of sequence in fragments shorter than    4379 bp
  50% of sequence in fragments shorter than    4845 bp
  55% of sequence in fragments shorter than    5346 bp
  60% of sequence in fragments shorter than    5902 bp
  65% of sequence in fragments shorter than    6523 bp
  70% of sequence in fragments shorter than    7234 bp
  75% of sequence in fragments shorter than    8069 bp
  80% of sequence in fragments shorter than    9096 bp
  85% of sequence in fragments shorter than   10431 bp
  90% of sequence in fragments shorter than   12401 bp
  95% of sequence in fragments shorter than   16295 bp

# BsaAI.SapI (YACGTR+GCTCTTC) double digest, 1090 thousand fragments (713801+377161 sites), 2732 bp average fragment length
   5% of sequence in fragments shorter than     964 bp
  10% of sequence in fragments shorter than    1454 bp
  15% of sequence in fragments shorter than    1898 bp
  20% of sequence in fragments shorter than    2306 bp
  25% of sequence in fragments shorter than    2714 bp
  30% of sequence in fragments shorter than    3112 bp
  35% of sequence in fragments shorter than    3493 bp
  40% of sequence in fragments shorter than    3916 bp
  45% of sequence in fragments shorter than    4359 bp
  50% of sequence in fragments shorter than    4832 bp
  55% of sequence in fragments shorter than    5338 bp
  60% of sequence in fragments shorter than    5897 bp
  65% of sequence in fragments shorter than    6517 bp
  70% of sequence in fragments shorter than    7215 bp
  75% of sequence in fragments shorter than    8027 bp
  80% of sequence in fragments shorter than    9018 bp
  85% of sequence in fragments shorter than   10288 bp
  90% of sequence in fragments shorter than   12094 bp
  95% of sequence in fragments shorter than   15448 bp

# HindIII.KasI (AAGCTT+GGCGCC) double digest, 1090 thousand fragments (860365+230381 sites), 2733 bp average fragment length
   5% of sequence in fragments shorter than     959 bp
  10% of sequence in fragments shorter than    1447 bp
  15% of sequence in fragments shorter than    1887 bp
  20% of sequence in fragments shorter than    2261 bp
  25% of sequence in fragments shorter than    2656 bp
  30% of sequence in fragments shorter than    3043 bp
  35% of sequence in fragments shorter than    3443 bp
  40% of sequence in fragments shorter than    3851 bp
  45% of sequence in fragments shorter than    4277 bp
  50% of sequence in fragments shorter than    4724 bp
  55% of sequence in fragments shorter than    5205 bp
  60% of sequence in fragments shorter than    5726 bp
  65% of sequence in fragments shorter than    6315 bp
  70% of sequence in fragments shorter than    6971 bp
  75% of sequence in fragments shorter than    7740 bp
  80% of sequence in fragments shorter than    8672 bp
  85% of sequence in fragments shorter than    9846 bp
  90% of sequence in fragments shorter than   11508 bp
  95% of sequence in fragments shorter than   14662 bp

# BsaAI.SmaI (YACGTR+CCCGGG) double digest, 1090 thousand fragments (713801+376940 sites), 2733 bp average fragment length
   5% of sequence in fragments shorter than     932 bp
  10% of sequence in fragments shorter than    1434 bp
  15% of sequence in fragments shorter than    1901 bp
  20% of sequence in fragments shorter than    2336 bp
  25% of sequence in fragments shorter than    2773 bp
  30% of sequence in fragments shorter than    3220 bp
  35% of sequence in fragments shorter than    3657 bp
  40% of sequence in fragments shorter than    4136 bp
  45% of sequence in fragments shorter than    4632 bp
  50% of sequence in fragments shorter than    5174 bp
  55% of sequence in fragments shorter than    5755 bp
  60% of sequence in fragments shorter than    6398 bp
  65% of sequence in fragments shorter than    7116 bp
  70% of sequence in fragments shorter than    7945 bp
  75% of sequence in fragments shorter than    8918 bp
  80% of sequence in fragments shorter than   10120 bp
  85% of sequence in fragments shorter than   11684 bp
  90% of sequence in fragments shorter than   13931 bp
  95% of sequence in fragments shorter than   18095 bp

# ApaI.HaeII (GGGCCC+RGCGCY) double digest, 1089 thousand fragments (460339+630101 sites), 2733 bp average fragment length
   5% of sequence in fragments shorter than     789 bp
  10% of sequence in fragments shorter than    1432 bp
  15% of sequence in fragments shorter than    2075 bp
  20% of sequence in fragments shorter than    2726 bp
  25% of sequence in fragments shorter than    3409 bp
  30% of sequence in fragments shorter than    4117 bp
  35% of sequence in fragments shorter than    4868 bp
  40% of sequence in fragments shorter than    5667 bp
  45% of sequence in fragments shorter than    6516 bp
  50% of sequence in fragments shorter than    7441 bp
  55% of sequence in fragments shorter than    8462 bp
  60% of sequence in fragments shorter than    9576 bp
  65% of sequence in fragments shorter than   10839 bp
  70% of sequence in fragments shorter than   12313 bp
  75% of sequence in fragments shorter than   14050 bp
  80% of sequence in fragments shorter than   16183 bp
  85% of sequence in fragments shorter than   18930 bp
  90% of sequence in fragments shorter than   22855 bp
  95% of sequence in fragments shorter than   29761 bp

# AscI.PpuMI (GGCGCGCC+RGGWCCY) double digest, 1089 thousand fragments (4751+1085138 sites), 2735 bp average fragment length
   5% of sequence in fragments shorter than     888 bp
  10% of sequence in fragments shorter than    1442 bp
  15% of sequence in fragments shorter than    1948 bp
  20% of sequence in fragments shorter than    2442 bp
  25% of sequence in fragments shorter than    2945 bp
  30% of sequence in fragments shorter than    3443 bp
  35% of sequence in fragments shorter than    3913 bp
  40% of sequence in fragments shorter than    4467 bp
  45% of sequence in fragments shorter than    5061 bp
  50% of sequence in fragments shorter than    5705 bp
  55% of sequence in fragments shorter than    6403 bp
  60% of sequence in fragments shorter than    7182 bp
  65% of sequence in fragments shorter than    8055 bp
  70% of sequence in fragments shorter than    9051 bp
  75% of sequence in fragments shorter than   10254 bp
  80% of sequence in fragments shorter than   11715 bp
  85% of sequence in fragments shorter than   13654 bp
  90% of sequence in fragments shorter than   16573 bp
  95% of sequence in fragments shorter than   22648 bp

# AflII.EcoRV (CTTAAG+GATATC) double digest, 1089 thousand fragments (643284+446473 sites), 2735 bp average fragment length
   5% of sequence in fragments shorter than     955 bp
  10% of sequence in fragments shorter than    1418 bp
  15% of sequence in fragments shorter than    1847 bp
  20% of sequence in fragments shorter than    2248 bp
  25% of sequence in fragments shorter than    2633 bp
  30% of sequence in fragments shorter than    3020 bp
  35% of sequence in fragments shorter than    3424 bp
  40% of sequence in fragments shorter than    3837 bp
  45% of sequence in fragments shorter than    4275 bp
  50% of sequence in fragments shorter than    4735 bp
  55% of sequence in fragments shorter than    5234 bp
  60% of sequence in fragments shorter than    5786 bp
  65% of sequence in fragments shorter than    6410 bp
  70% of sequence in fragments shorter than    7120 bp
  75% of sequence in fragments shorter than    7965 bp
  80% of sequence in fragments shorter than    9013 bp
  85% of sequence in fragments shorter than   10402 bp
  90% of sequence in fragments shorter than   12523 bp
  95% of sequence in fragments shorter than   16891 bp

# AscI.PvuII (GGCGCGCC+CAGCTG) double digest, 1088 thousand fragments (4751+1084260 sites), 2737 bp average fragment length
   5% of sequence in fragments shorter than     916 bp
  10% of sequence in fragments shorter than    1430 bp
  15% of sequence in fragments shorter than    1899 bp
  20% of sequence in fragments shorter than    2350 bp
  25% of sequence in fragments shorter than    2791 bp
  30% of sequence in fragments shorter than    3242 bp
  35% of sequence in fragments shorter than    3712 bp
  40% of sequence in fragments shorter than    4217 bp
  45% of sequence in fragments shorter than    4744 bp
  50% of sequence in fragments shorter than    5323 bp
  55% of sequence in fragments shorter than    5940 bp
  60% of sequence in fragments shorter than    6614 bp
  65% of sequence in fragments shorter than    7356 bp
  70% of sequence in fragments shorter than    8225 bp
  75% of sequence in fragments shorter than    9240 bp
  80% of sequence in fragments shorter than   10526 bp
  85% of sequence in fragments shorter than   12166 bp
  90% of sequence in fragments shorter than   14675 bp
  95% of sequence in fragments shorter than   19537 bp

# HindIII.SwaI (AAGCTT+ATTTAAAT) double digest, 1086 thousand fragments (860365+226602 sites), 2742 bp average fragment length
   5% of sequence in fragments shorter than     957 bp
  10% of sequence in fragments shorter than    1447 bp
  15% of sequence in fragments shorter than    1888 bp
  20% of sequence in fragments shorter than    2258 bp
  25% of sequence in fragments shorter than    2653 bp
  30% of sequence in fragments shorter than    3043 bp
  35% of sequence in fragments shorter than    3448 bp
  40% of sequence in fragments shorter than    3867 bp
  45% of sequence in fragments shorter than    4309 bp
  50% of sequence in fragments shorter than    4774 bp
  55% of sequence in fragments shorter than    5276 bp
  60% of sequence in fragments shorter than    5834 bp
  65% of sequence in fragments shorter than    6464 bp
  70% of sequence in fragments shorter than    7177 bp
  75% of sequence in fragments shorter than    8032 bp
  80% of sequence in fragments shorter than    9087 bp
  85% of sequence in fragments shorter than   10478 bp
  90% of sequence in fragments shorter than   12578 bp
  95% of sequence in fragments shorter than   16920 bp

# BsrBI.XbaI (CCGCTC+TCTAGA) double digest, 1086 thousand fragments (235523+850998 sites), 2743 bp average fragment length
   5% of sequence in fragments shorter than     935 bp
  10% of sequence in fragments shorter than    1422 bp
  15% of sequence in fragments shorter than    1880 bp
  20% of sequence in fragments shorter than    2282 bp
  25% of sequence in fragments shorter than    2702 bp
  30% of sequence in fragments shorter than    3110 bp
  35% of sequence in fragments shorter than    3529 bp
  40% of sequence in fragments shorter than    3961 bp
  45% of sequence in fragments shorter than    4409 bp
  50% of sequence in fragments shorter than    4879 bp
  55% of sequence in fragments shorter than    5379 bp
  60% of sequence in fragments shorter than    5928 bp
  65% of sequence in fragments shorter than    6531 bp
  70% of sequence in fragments shorter than    7209 bp
  75% of sequence in fragments shorter than    7983 bp
  80% of sequence in fragments shorter than    8917 bp
  85% of sequence in fragments shorter than   10122 bp
  90% of sequence in fragments shorter than   11796 bp
  95% of sequence in fragments shorter than   14686 bp

# BciVI.HpaI (GTATCC+GTTAAC) double digest, 1085 thousand fragments (696093+389418 sites), 2746 bp average fragment length
   5% of sequence in fragments shorter than     974 bp
  10% of sequence in fragments shorter than    1467 bp
  15% of sequence in fragments shorter than    1870 bp
  20% of sequence in fragments shorter than    2257 bp
  25% of sequence in fragments shorter than    2645 bp
  30% of sequence in fragments shorter than    3036 bp
  35% of sequence in fragments shorter than    3428 bp
  40% of sequence in fragments shorter than    3835 bp
  45% of sequence in fragments shorter than    4262 bp
  50% of sequence in fragments shorter than    4718 bp
  55% of sequence in fragments shorter than    5201 bp
  60% of sequence in fragments shorter than    5733 bp
  65% of sequence in fragments shorter than    6328 bp
  70% of sequence in fragments shorter than    7006 bp
  75% of sequence in fragments shorter than    7801 bp
  80% of sequence in fragments shorter than    8770 bp
  85% of sequence in fragments shorter than   10038 bp
  90% of sequence in fragments shorter than   11918 bp
  95% of sequence in fragments shorter than   15565 bp

# PacI.StuI (TTAATTAA+AGGCCT) double digest, 1084 thousand fragments (159455+925729 sites), 2747 bp average fragment length
   5% of sequence in fragments shorter than     861 bp
  10% of sequence in fragments shorter than    1425 bp
  15% of sequence in fragments shorter than    1943 bp
  20% of sequence in fragments shorter than    2406 bp
  25% of sequence in fragments shorter than    2890 bp
  30% of sequence in fragments shorter than    3370 bp
  35% of sequence in fragments shorter than    3856 bp
  40% of sequence in fragments shorter than    4367 bp
  45% of sequence in fragments shorter than    4897 bp
  50% of sequence in fragments shorter than    5458 bp
  55% of sequence in fragments shorter than    6056 bp
  60% of sequence in fragments shorter than    6709 bp
  65% of sequence in fragments shorter than    7435 bp
  70% of sequence in fragments shorter than    8247 bp
  75% of sequence in fragments shorter than    9196 bp
  80% of sequence in fragments shorter than   10320 bp
  85% of sequence in fragments shorter than   11761 bp
  90% of sequence in fragments shorter than   13749 bp
  95% of sequence in fragments shorter than   17106 bp

# Hpy99I.StuI (CGWCG+AGGCCT) double digest, 1083 thousand fragments (157917+925729 sites), 2751 bp average fragment length
   5% of sequence in fragments shorter than     813 bp
  10% of sequence in fragments shorter than    1398 bp
  15% of sequence in fragments shorter than    1957 bp
  20% of sequence in fragments shorter than    2471 bp
  25% of sequence in fragments shorter than    3011 bp
  30% of sequence in fragments shorter than    3556 bp
  35% of sequence in fragments shorter than    4114 bp
  40% of sequence in fragments shorter than    4698 bp
  45% of sequence in fragments shorter than    5312 bp
  50% of sequence in fragments shorter than    5964 bp
  55% of sequence in fragments shorter than    6676 bp
  60% of sequence in fragments shorter than    7449 bp
  65% of sequence in fragments shorter than    8301 bp
  70% of sequence in fragments shorter than    9275 bp
  75% of sequence in fragments shorter than   10402 bp
  80% of sequence in fragments shorter than   11774 bp
  85% of sequence in fragments shorter than   13507 bp
  90% of sequence in fragments shorter than   15940 bp
  95% of sequence in fragments shorter than   20016 bp

# BsrBI.EcoRI (CCGCTC+GAATTC) double digest, 1082 thousand fragments (235523+847401 sites), 2753 bp average fragment length
   5% of sequence in fragments shorter than     920 bp
  10% of sequence in fragments shorter than    1478 bp
  15% of sequence in fragments shorter than    1955 bp
  20% of sequence in fragments shorter than    2398 bp
  25% of sequence in fragments shorter than    2812 bp
  30% of sequence in fragments shorter than    3230 bp
  35% of sequence in fragments shorter than    3650 bp
  40% of sequence in fragments shorter than    4090 bp
  45% of sequence in fragments shorter than    4544 bp
  50% of sequence in fragments shorter than    5028 bp
  55% of sequence in fragments shorter than    5542 bp
  60% of sequence in fragments shorter than    6098 bp
  65% of sequence in fragments shorter than    6716 bp
  70% of sequence in fragments shorter than    7417 bp
  75% of sequence in fragments shorter than    8220 bp
  80% of sequence in fragments shorter than    9176 bp
  85% of sequence in fragments shorter than   10412 bp
  90% of sequence in fragments shorter than   12133 bp
  95% of sequence in fragments shorter than   15088 bp

# BceAI.SapI (ACGGC+GCTCTTC) double digest, 1081 thousand fragments (705178+377161 sites), 2754 bp average fragment length
   5% of sequence in fragments shorter than     902 bp
  10% of sequence in fragments shorter than    1441 bp
  15% of sequence in fragments shorter than    1940 bp
  20% of sequence in fragments shorter than    2419 bp
  25% of sequence in fragments shorter than    2898 bp
  30% of sequence in fragments shorter than    3385 bp
  35% of sequence in fragments shorter than    3884 bp
  40% of sequence in fragments shorter than    4408 bp
  45% of sequence in fragments shorter than    4962 bp
  50% of sequence in fragments shorter than    5553 bp
  55% of sequence in fragments shorter than    6191 bp
  60% of sequence in fragments shorter than    6903 bp
  65% of sequence in fragments shorter than    7703 bp
  70% of sequence in fragments shorter than    8604 bp
  75% of sequence in fragments shorter than    9676 bp
  80% of sequence in fragments shorter than   10975 bp
  85% of sequence in fragments shorter than   12651 bp
  90% of sequence in fragments shorter than   15151 bp
  95% of sequence in fragments shorter than   19644 bp

# BceAI.SmaI (ACGGC+CCCGGG) double digest, 1081 thousand fragments (705178+376940 sites), 2754 bp average fragment length
   5% of sequence in fragments shorter than     877 bp
  10% of sequence in fragments shorter than    1463 bp
  15% of sequence in fragments shorter than    2013 bp
  20% of sequence in fragments shorter than    2563 bp
  25% of sequence in fragments shorter than    3119 bp
  30% of sequence in fragments shorter than    3702 bp
  35% of sequence in fragments shorter than    4313 bp
  40% of sequence in fragments shorter than    4969 bp
  45% of sequence in fragments shorter than    5676 bp
  50% of sequence in fragments shorter than    6426 bp
  55% of sequence in fragments shorter than    7264 bp
  60% of sequence in fragments shorter than    8199 bp
  65% of sequence in fragments shorter than    9259 bp
  70% of sequence in fragments shorter than   10493 bp
  75% of sequence in fragments shorter than   11953 bp
  80% of sequence in fragments shorter than   13776 bp
  85% of sequence in fragments shorter than   16175 bp
  90% of sequence in fragments shorter than   19677 bp
  95% of sequence in fragments shorter than   26292 bp

# KasI.XbaI (GGCGCC+TCTAGA) double digest, 1080 thousand fragments (230381+850998 sites), 2756 bp average fragment length
   5% of sequence in fragments shorter than     943 bp
  10% of sequence in fragments shorter than    1426 bp
  15% of sequence in fragments shorter than    1876 bp
  20% of sequence in fragments shorter than    2271 bp
  25% of sequence in fragments shorter than    2682 bp
  30% of sequence in fragments shorter than    3064 bp
  35% of sequence in fragments shorter than    3475 bp
  40% of sequence in fragments shorter than    3898 bp
  45% of sequence in fragments shorter than    4338 bp
  50% of sequence in fragments shorter than    4802 bp
  55% of sequence in fragments shorter than    5290 bp
  60% of sequence in fragments shorter than    5827 bp
  65% of sequence in fragments shorter than    6416 bp
  70% of sequence in fragments shorter than    7082 bp
  75% of sequence in fragments shorter than    7848 bp
  80% of sequence in fragments shorter than    8776 bp
  85% of sequence in fragments shorter than    9949 bp
  90% of sequence in fragments shorter than   11622 bp
  95% of sequence in fragments shorter than   14527 bp

# BamHI.BsaAI (GGATCC+YACGTR) double digest, 1079 thousand fragments (365999+713801 sites), 2760 bp average fragment length
   5% of sequence in fragments shorter than     962 bp
  10% of sequence in fragments shorter than    1459 bp
  15% of sequence in fragments shorter than    1901 bp
  20% of sequence in fragments shorter than    2323 bp
  25% of sequence in fragments shorter than    2739 bp
  30% of sequence in fragments shorter than    3123 bp
  35% of sequence in fragments shorter than    3540 bp
  40% of sequence in fragments shorter than    3965 bp
  45% of sequence in fragments shorter than    4425 bp
  50% of sequence in fragments shorter than    4910 bp
  55% of sequence in fragments shorter than    5429 bp
  60% of sequence in fragments shorter than    5994 bp
  65% of sequence in fragments shorter than    6623 bp
  70% of sequence in fragments shorter than    7326 bp
  75% of sequence in fragments shorter than    8160 bp
  80% of sequence in fragments shorter than    9168 bp
  85% of sequence in fragments shorter than   10468 bp
  90% of sequence in fragments shorter than   12311 bp
  95% of sequence in fragments shorter than   15710 bp

# EcoRI.KasI (GAATTC+GGCGCC) double digest, 1077 thousand fragments (847401+230381 sites), 2766 bp average fragment length
   5% of sequence in fragments shorter than     930 bp
  10% of sequence in fragments shorter than    1474 bp
  15% of sequence in fragments shorter than    1943 bp
  20% of sequence in fragments shorter than    2378 bp
  25% of sequence in fragments shorter than    2791 bp
  30% of sequence in fragments shorter than    3185 bp
  35% of sequence in fragments shorter than    3600 bp
  40% of sequence in fragments shorter than    4035 bp
  45% of sequence in fragments shorter than    4484 bp
  50% of sequence in fragments shorter than    4960 bp
  55% of sequence in fragments shorter than    5469 bp
  60% of sequence in fragments shorter than    6012 bp
  65% of sequence in fragments shorter than    6623 bp
  70% of sequence in fragments shorter than    7310 bp
  75% of sequence in fragments shorter than    8100 bp
  80% of sequence in fragments shorter than    9051 bp
  85% of sequence in fragments shorter than   10258 bp
  90% of sequence in fragments shorter than   11952 bp
  95% of sequence in fragments shorter than   14971 bp

# SwaI.XbaI (ATTTAAAT+TCTAGA) double digest, 1077 thousand fragments (226602+850998 sites), 2766 bp average fragment length
   5% of sequence in fragments shorter than     936 bp
  10% of sequence in fragments shorter than    1413 bp
  15% of sequence in fragments shorter than    1860 bp
  20% of sequence in fragments shorter than    2259 bp
  25% of sequence in fragments shorter than    2675 bp
  30% of sequence in fragments shorter than    3088 bp
  35% of sequence in fragments shorter than    3511 bp
  40% of sequence in fragments shorter than    3949 bp
  45% of sequence in fragments shorter than    4403 bp
  50% of sequence in fragments shorter than    4887 bp
  55% of sequence in fragments shorter than    5412 bp
  60% of sequence in fragments shorter than    5991 bp
  65% of sequence in fragments shorter than    6637 bp
  70% of sequence in fragments shorter than    7386 bp
  75% of sequence in fragments shorter than    8263 bp
  80% of sequence in fragments shorter than    9337 bp
  85% of sequence in fragments shorter than   10764 bp
  90% of sequence in fragments shorter than   12840 bp
  95% of sequence in fragments shorter than   16849 bp

# AatII.AvaI (GACGTC+CYCGRG) double digest, 1076 thousand fragments (71089+1005714 sites), 2768 bp average fragment length
   5% of sequence in fragments shorter than     884 bp
  10% of sequence in fragments shorter than    1469 bp
  15% of sequence in fragments shorter than    2026 bp
  20% of sequence in fragments shorter than    2591 bp
  25% of sequence in fragments shorter than    3177 bp
  30% of sequence in fragments shorter than    3806 bp
  35% of sequence in fragments shorter than    4473 bp
  40% of sequence in fragments shorter than    5197 bp
  45% of sequence in fragments shorter than    5976 bp
  50% of sequence in fragments shorter than    6845 bp
  55% of sequence in fragments shorter than    7808 bp
  60% of sequence in fragments shorter than    8910 bp
  65% of sequence in fragments shorter than   10161 bp
  70% of sequence in fragments shorter than   11606 bp
  75% of sequence in fragments shorter than   13357 bp
  80% of sequence in fragments shorter than   15547 bp
  85% of sequence in fragments shorter than   18432 bp
  90% of sequence in fragments shorter than   22878 bp
  95% of sequence in fragments shorter than   31672 bp

# EcoRV.HaeII (GATATC+RGCGCY) double digest, 1076 thousand fragments (446473+630101 sites), 2769 bp average fragment length
   5% of sequence in fragments shorter than     883 bp
  10% of sequence in fragments shorter than    1495 bp
  15% of sequence in fragments shorter than    2031 bp
  20% of sequence in fragments shorter than    2513 bp
  25% of sequence in fragments shorter than    3008 bp
  30% of sequence in fragments shorter than    3495 bp
  35% of sequence in fragments shorter than    3993 bp
  40% of sequence in fragments shorter than    4499 bp
  45% of sequence in fragments shorter than    5027 bp
  50% of sequence in fragments shorter than    5587 bp
  55% of sequence in fragments shorter than    6188 bp
  60% of sequence in fragments shorter than    6839 bp
  65% of sequence in fragments shorter than    7566 bp
  70% of sequence in fragments shorter than    8369 bp
  75% of sequence in fragments shorter than    9318 bp
  80% of sequence in fragments shorter than   10449 bp
  85% of sequence in fragments shorter than   11892 bp
  90% of sequence in fragments shorter than   13914 bp
  95% of sequence in fragments shorter than   17328 bp

# AclI.NdeI (AACGTT+CATATG) double digest, 1075 thousand fragments (172706+903360 sites), 2770 bp average fragment length
   5% of sequence in fragments shorter than     944 bp
  10% of sequence in fragments shorter than    1429 bp
  15% of sequence in fragments shorter than    1894 bp
  20% of sequence in fragments shorter than    2325 bp
  25% of sequence in fragments shorter than    2758 bp
  30% of sequence in fragments shorter than    3170 bp
  35% of sequence in fragments shorter than    3610 bp
  40% of sequence in fragments shorter than    4076 bp
  45% of sequence in fragments shorter than    4556 bp
  50% of sequence in fragments shorter than    5066 bp
  55% of sequence in fragments shorter than    5614 bp
  60% of sequence in fragments shorter than    6218 bp
  65% of sequence in fragments shorter than    6898 bp
  70% of sequence in fragments shorter than    7686 bp
  75% of sequence in fragments shorter than    8634 bp
  80% of sequence in fragments shorter than    9808 bp
  85% of sequence in fragments shorter than   11371 bp
  90% of sequence in fragments shorter than   13819 bp
  95% of sequence in fragments shorter than   18896 bp

# BclI.BstEII (TGATCA+GGTNACC) double digest, 1074 thousand fragments (738785+335865 sites), 2773 bp average fragment length
   5% of sequence in fragments shorter than     973 bp
  10% of sequence in fragments shorter than    1478 bp
  15% of sequence in fragments shorter than    1900 bp
  20% of sequence in fragments shorter than    2271 bp
  25% of sequence in fragments shorter than    2660 bp
  30% of sequence in fragments shorter than    3049 bp
  35% of sequence in fragments shorter than    3444 bp
  40% of sequence in fragments shorter than    3846 bp
  45% of sequence in fragments shorter than    4261 bp
  50% of sequence in fragments shorter than    4713 bp
  55% of sequence in fragments shorter than    5189 bp
  60% of sequence in fragments shorter than    5713 bp
  65% of sequence in fragments shorter than    6286 bp
  70% of sequence in fragments shorter than    6947 bp
  75% of sequence in fragments shorter than    7709 bp
  80% of sequence in fragments shorter than    8636 bp
  85% of sequence in fragments shorter than    9861 bp
  90% of sequence in fragments shorter than   11641 bp
  95% of sequence in fragments shorter than   15231 bp

# EcoRI.SwaI (GAATTC+ATTTAAAT) double digest, 1073 thousand fragments (847401+226602 sites), 2775 bp average fragment length
   5% of sequence in fragments shorter than     927 bp
  10% of sequence in fragments shorter than    1467 bp
  15% of sequence in fragments shorter than    1934 bp
  20% of sequence in fragments shorter than    2370 bp
  25% of sequence in fragments shorter than    2784 bp
  30% of sequence in fragments shorter than    3201 bp
  35% of sequence in fragments shorter than    3615 bp
  40% of sequence in fragments shorter than    4056 bp
  45% of sequence in fragments shorter than    4514 bp
  50% of sequence in fragments shorter than    5010 bp
  55% of sequence in fragments shorter than    5541 bp
  60% of sequence in fragments shorter than    6115 bp
  65% of sequence in fragments shorter than    6763 bp
  70% of sequence in fragments shorter than    7501 bp
  75% of sequence in fragments shorter than    8371 bp
  80% of sequence in fragments shorter than    9434 bp
  85% of sequence in fragments shorter than   10852 bp
  90% of sequence in fragments shorter than   12872 bp
  95% of sequence in fragments shorter than   16731 bp

# BciVI.SapI (GTATCC+GCTCTTC) double digest, 1072 thousand fragments (696093+377161 sites), 2777 bp average fragment length
   5% of sequence in fragments shorter than     975 bp
  10% of sequence in fragments shorter than    1477 bp
  15% of sequence in fragments shorter than    1892 bp
  20% of sequence in fragments shorter than    2280 bp
  25% of sequence in fragments shorter than    2671 bp
  30% of sequence in fragments shorter than    3064 bp
  35% of sequence in fragments shorter than    3465 bp
  40% of sequence in fragments shorter than    3877 bp
  45% of sequence in fragments shorter than    4301 bp
  50% of sequence in fragments shorter than    4759 bp
  55% of sequence in fragments shorter than    5248 bp
  60% of sequence in fragments shorter than    5788 bp
  65% of sequence in fragments shorter than    6377 bp
  70% of sequence in fragments shorter than    7040 bp
  75% of sequence in fragments shorter than    7824 bp
  80% of sequence in fragments shorter than    8781 bp
  85% of sequence in fragments shorter than    9998 bp
  90% of sequence in fragments shorter than   11767 bp
  95% of sequence in fragments shorter than   15130 bp

# BciVI.SmaI (GTATCC+CCCGGG) double digest, 1072 thousand fragments (696093+376940 sites), 2778 bp average fragment length
   5% of sequence in fragments shorter than     967 bp
  10% of sequence in fragments shorter than    1479 bp
  15% of sequence in fragments shorter than    1905 bp
  20% of sequence in fragments shorter than    2313 bp
  25% of sequence in fragments shorter than    2721 bp
  30% of sequence in fragments shorter than    3127 bp
  35% of sequence in fragments shorter than    3544 bp
  40% of sequence in fragments shorter than    3970 bp
  45% of sequence in fragments shorter than    4407 bp
  50% of sequence in fragments shorter than    4883 bp
  55% of sequence in fragments shorter than    5393 bp
  60% of sequence in fragments shorter than    5947 bp
  65% of sequence in fragments shorter than    6566 bp
  70% of sequence in fragments shorter than    7264 bp
  75% of sequence in fragments shorter than    8086 bp
  80% of sequence in fragments shorter than    9081 bp
  85% of sequence in fragments shorter than   10348 bp
  90% of sequence in fragments shorter than   12210 bp
  95% of sequence in fragments shorter than   15724 bp

# AvaI.SacII (CYCGRG+CCGCGG) double digest, 1072 thousand fragments (1005714+66901 sites), 2779 bp average fragment length
   5% of sequence in fragments shorter than     905 bp
  10% of sequence in fragments shorter than    1522 bp
  15% of sequence in fragments shorter than    2116 bp
  20% of sequence in fragments shorter than    2720 bp
  25% of sequence in fragments shorter than    3354 bp
  30% of sequence in fragments shorter than    4030 bp
  35% of sequence in fragments shorter than    4753 bp
  40% of sequence in fragments shorter than    5530 bp
  45% of sequence in fragments shorter than    6382 bp
  50% of sequence in fragments shorter than    7329 bp
  55% of sequence in fragments shorter than    8380 bp
  60% of sequence in fragments shorter than    9583 bp
  65% of sequence in fragments shorter than   10937 bp
  70% of sequence in fragments shorter than   12542 bp
  75% of sequence in fragments shorter than   14477 bp
  80% of sequence in fragments shorter than   16885 bp
  85% of sequence in fragments shorter than   20090 bp
  90% of sequence in fragments shorter than   24978 bp
  95% of sequence in fragments shorter than   35220 bp

# BglII.SexAI (AGATCT+ACCWGGT) double digest, 1072 thousand fragments (774732+297900 sites), 2779 bp average fragment length
   5% of sequence in fragments shorter than     972 bp
  10% of sequence in fragments shorter than    1471 bp
  15% of sequence in fragments shorter than    1889 bp
  20% of sequence in fragments shorter than    2291 bp
  25% of sequence in fragments shorter than    2688 bp
  30% of sequence in fragments shorter than    3093 bp
  35% of sequence in fragments shorter than    3497 bp
  40% of sequence in fragments shorter than    3912 bp
  45% of sequence in fragments shorter than    4341 bp
  50% of sequence in fragments shorter than    4795 bp
  55% of sequence in fragments shorter than    5280 bp
  60% of sequence in fragments shorter than    5813 bp
  65% of sequence in fragments shorter than    6404 bp
  70% of sequence in fragments shorter than    7077 bp
  75% of sequence in fragments shorter than    7869 bp
  80% of sequence in fragments shorter than    8819 bp
  85% of sequence in fragments shorter than   10073 bp
  90% of sequence in fragments shorter than   11869 bp
  95% of sequence in fragments shorter than   15519 bp

# BamHI.BceAI (GGATCC+ACGGC) double digest, 1070 thousand fragments (365999+705178 sites), 2783 bp average fragment length
   5% of sequence in fragments shorter than     924 bp
  10% of sequence in fragments shorter than    1482 bp
  15% of sequence in fragments shorter than    1989 bp
  20% of sequence in fragments shorter than    2465 bp
  25% of sequence in fragments shorter than    2952 bp
  30% of sequence in fragments shorter than    3449 bp
  35% of sequence in fragments shorter than    3958 bp
  40% of sequence in fragments shorter than    4469 bp
  45% of sequence in fragments shorter than    5019 bp
  50% of sequence in fragments shorter than    5630 bp
  55% of sequence in fragments shorter than    6294 bp
  60% of sequence in fragments shorter than    7037 bp
  65% of sequence in fragments shorter than    7864 bp
  70% of sequence in fragments shorter than    8792 bp
  75% of sequence in fragments shorter than    9913 bp
  80% of sequence in fragments shorter than   11271 bp
  85% of sequence in fragments shorter than   13042 bp
  90% of sequence in fragments shorter than   15618 bp
  95% of sequence in fragments shorter than   20317 bp

# BstEII.FnuDII (GGTNACC+CGCG) double digest, 1069 thousand fragments (335865+733940 sites), 2786 bp average fragment length
   5% of sequence in fragments shorter than    1105 bp
  10% of sequence in fragments shorter than    1870 bp
  15% of sequence in fragments shorter than    2567 bp
  20% of sequence in fragments shorter than    3238 bp
  25% of sequence in fragments shorter than    3918 bp
  30% of sequence in fragments shorter than    4620 bp
  35% of sequence in fragments shorter than    5336 bp
  40% of sequence in fragments shorter than    6095 bp
  45% of sequence in fragments shorter than    6889 bp
  50% of sequence in fragments shorter than    7748 bp
  55% of sequence in fragments shorter than    8699 bp
  60% of sequence in fragments shorter than    9756 bp
  65% of sequence in fragments shorter than   10953 bp
  70% of sequence in fragments shorter than   12326 bp
  75% of sequence in fragments shorter than   13953 bp
  80% of sequence in fragments shorter than   15950 bp
  85% of sequence in fragments shorter than   18643 bp
  90% of sequence in fragments shorter than   22631 bp
  95% of sequence in fragments shorter than   30514 bp

# ApaLI.HgaI (GTGCAC+GACGC) double digest, 1067 thousand fragments (496255+571889 sites), 2791 bp average fragment length
   5% of sequence in fragments shorter than     942 bp
  10% of sequence in fragments shorter than    1506 bp
  15% of sequence in fragments shorter than    2011 bp
  20% of sequence in fragments shorter than    2499 bp
  25% of sequence in fragments shorter than    2957 bp
  30% of sequence in fragments shorter than    3437 bp
  35% of sequence in fragments shorter than    3952 bp
  40% of sequence in fragments shorter than    4490 bp
  45% of sequence in fragments shorter than    5062 bp
  50% of sequence in fragments shorter than    5663 bp
  55% of sequence in fragments shorter than    6301 bp
  60% of sequence in fragments shorter than    7028 bp
  65% of sequence in fragments shorter than    7845 bp
  70% of sequence in fragments shorter than    8788 bp
  75% of sequence in fragments shorter than    9872 bp
  80% of sequence in fragments shorter than   11231 bp
  85% of sequence in fragments shorter than   12978 bp
  90% of sequence in fragments shorter than   15499 bp
  95% of sequence in fragments shorter than   20345 bp

# ApaLI.EciI (GTGCAC+GGCGGA) double digest, 1067 thousand fragments (496255+571273 sites), 2792 bp average fragment length
   5% of sequence in fragments shorter than     938 bp
  10% of sequence in fragments shorter than    1481 bp
  15% of sequence in fragments shorter than    1968 bp
  20% of sequence in fragments shorter than    2440 bp
  25% of sequence in fragments shorter than    2916 bp
  30% of sequence in fragments shorter than    3401 bp
  35% of sequence in fragments shorter than    3912 bp
  40% of sequence in fragments shorter than    4451 bp
  45% of sequence in fragments shorter than    5029 bp
  50% of sequence in fragments shorter than    5646 bp
  55% of sequence in fragments shorter than    6298 bp
  60% of sequence in fragments shorter than    7037 bp
  65% of sequence in fragments shorter than    7889 bp
  70% of sequence in fragments shorter than    8865 bp
  75% of sequence in fragments shorter than   10020 bp
  80% of sequence in fragments shorter than   11453 bp
  85% of sequence in fragments shorter than   13358 bp
  90% of sequence in fragments shorter than   16129 bp
  95% of sequence in fragments shorter than   21568 bp

# AvaIII.SnaBI (ATGCAT+TACGTA) double digest, 1065 thousand fragments (933357+132299 sites), 2797 bp average fragment length
   5% of sequence in fragments shorter than     992 bp
  10% of sequence in fragments shorter than    1504 bp
  15% of sequence in fragments shorter than    1959 bp
  20% of sequence in fragments shorter than    2379 bp
  25% of sequence in fragments shorter than    2789 bp
  30% of sequence in fragments shorter than    3212 bp
  35% of sequence in fragments shorter than    3649 bp
  40% of sequence in fragments shorter than    4105 bp
  45% of sequence in fragments shorter than    4583 bp
  50% of sequence in fragments shorter than    5095 bp
  55% of sequence in fragments shorter than    5653 bp
  60% of sequence in fragments shorter than    6266 bp
  65% of sequence in fragments shorter than    6963 bp
  70% of sequence in fragments shorter than    7756 bp
  75% of sequence in fragments shorter than    8679 bp
  80% of sequence in fragments shorter than    9827 bp
  85% of sequence in fragments shorter than   11362 bp
  90% of sequence in fragments shorter than   13634 bp
  95% of sequence in fragments shorter than   18349 bp

# AvaI.PspXI (CYCGRG+VCTCGAGB) double digest, 1064 thousand fragments (1005714+59193 sites), 2799 bp average fragment length
   5% of sequence in fragments shorter than     948 bp
  10% of sequence in fragments shorter than    1573 bp
  15% of sequence in fragments shorter than    2173 bp
  20% of sequence in fragments shorter than    2785 bp
  25% of sequence in fragments shorter than    3429 bp
  30% of sequence in fragments shorter than    4114 bp
  35% of sequence in fragments shorter than    4844 bp
  40% of sequence in fragments shorter than    5627 bp
  45% of sequence in fragments shorter than    6492 bp
  50% of sequence in fragments shorter than    7450 bp
  55% of sequence in fragments shorter than    8513 bp
  60% of sequence in fragments shorter than    9729 bp
  65% of sequence in fragments shorter than   11102 bp
  70% of sequence in fragments shorter than   12719 bp
  75% of sequence in fragments shorter than   14684 bp
  80% of sequence in fragments shorter than   17113 bp
  85% of sequence in fragments shorter than   20358 bp
  90% of sequence in fragments shorter than   25331 bp
  95% of sequence in fragments shorter than   35719 bp

# BspHI.ClaI (TCATGA+ATCGAT) double digest, 1064 thousand fragments (978290+86477 sites), 2799 bp average fragment length
   5% of sequence in fragments shorter than     982 bp
  10% of sequence in fragments shorter than    1494 bp
  15% of sequence in fragments shorter than    1933 bp
  20% of sequence in fragments shorter than    2323 bp
  25% of sequence in fragments shorter than    2721 bp
  30% of sequence in fragments shorter than    3117 bp
  35% of sequence in fragments shorter than    3526 bp
  40% of sequence in fragments shorter than    3950 bp
  45% of sequence in fragments shorter than    4389 bp
  50% of sequence in fragments shorter than    4853 bp
  55% of sequence in fragments shorter than    5359 bp
  60% of sequence in fragments shorter than    5909 bp
  65% of sequence in fragments shorter than    6519 bp
  70% of sequence in fragments shorter than    7217 bp
  75% of sequence in fragments shorter than    8041 bp
  80% of sequence in fragments shorter than    9037 bp
  85% of sequence in fragments shorter than   10380 bp
  90% of sequence in fragments shorter than   12382 bp
  95% of sequence in fragments shorter than   16450 bp

# ApaI.SacI (GGGCCC+GAGCTC) double digest, 1063 thousand fragments (460339+603323 sites), 2802 bp average fragment length
   5% of sequence in fragments shorter than     900 bp
  10% of sequence in fragments shorter than    1459 bp
  15% of sequence in fragments shorter than    1981 bp
  20% of sequence in fragments shorter than    2488 bp
  25% of sequence in fragments shorter than    3007 bp
  30% of sequence in fragments shorter than    3540 bp
  35% of sequence in fragments shorter than    4103 bp
  40% of sequence in fragments shorter than    4678 bp
  45% of sequence in fragments shorter than    5294 bp
  50% of sequence in fragments shorter than    5950 bp
  55% of sequence in fragments shorter than    6669 bp
  60% of sequence in fragments shorter than    7466 bp
  65% of sequence in fragments shorter than    8355 bp
  70% of sequence in fragments shorter than    9388 bp
  75% of sequence in fragments shorter than   10608 bp
  80% of sequence in fragments shorter than   12098 bp
  85% of sequence in fragments shorter than   14057 bp
  90% of sequence in fragments shorter than   16989 bp
  95% of sequence in fragments shorter than   22934 bp

# NdeI.PacI (CATATG+TTAATTAA) double digest, 1062 thousand fragments (903360+159455 sites), 2804 bp average fragment length
   5% of sequence in fragments shorter than     949 bp
  10% of sequence in fragments shorter than    1452 bp
  15% of sequence in fragments shorter than    1924 bp
  20% of sequence in fragments shorter than    2374 bp
  25% of sequence in fragments shorter than    2817 bp
  30% of sequence in fragments shorter than    3263 bp
  35% of sequence in fragments shorter than    3728 bp
  40% of sequence in fragments shorter than    4205 bp
  45% of sequence in fragments shorter than    4704 bp
  50% of sequence in fragments shorter than    5228 bp
  55% of sequence in fragments shorter than    5806 bp
  60% of sequence in fragments shorter than    6439 bp
  65% of sequence in fragments shorter than    7168 bp
  70% of sequence in fragments shorter than    8016 bp
  75% of sequence in fragments shorter than    9055 bp
  80% of sequence in fragments shorter than   10354 bp
  85% of sequence in fragments shorter than   12178 bp
  90% of sequence in fragments shorter than   15105 bp
  95% of sequence in fragments shorter than   21932 bp

# BglII.KpnI (AGATCT+GGTACC) double digest, 1062 thousand fragments (774732+287974 sites), 2805 bp average fragment length
   5% of sequence in fragments shorter than     978 bp
  10% of sequence in fragments shorter than    1437 bp
  15% of sequence in fragments shorter than    1868 bp
  20% of sequence in fragments shorter than    2281 bp
  25% of sequence in fragments shorter than    2688 bp
  30% of sequence in fragments shorter than    3095 bp
  35% of sequence in fragments shorter than    3507 bp
  40% of sequence in fragments shorter than    3934 bp
  45% of sequence in fragments shorter than    4380 bp
  50% of sequence in fragments shorter than    4849 bp
  55% of sequence in fragments shorter than    5356 bp
  60% of sequence in fragments shorter than    5917 bp
  65% of sequence in fragments shorter than    6532 bp
  70% of sequence in fragments shorter than    7229 bp
  75% of sequence in fragments shorter than    8051 bp
  80% of sequence in fragments shorter than    9051 bp
  85% of sequence in fragments shorter than   10383 bp
  90% of sequence in fragments shorter than   12318 bp
  95% of sequence in fragments shorter than   16140 bp

# BglII.NheI (AGATCT+GCTAGC) double digest, 1061 thousand fragments (774732+287384 sites), 2806 bp average fragment length
   5% of sequence in fragments shorter than     988 bp
  10% of sequence in fragments shorter than    1502 bp
  15% of sequence in fragments shorter than    1883 bp
  20% of sequence in fragments shorter than    2286 bp
  25% of sequence in fragments shorter than    2693 bp
  30% of sequence in fragments shorter than    3101 bp
  35% of sequence in fragments shorter than    3515 bp
  40% of sequence in fragments shorter than    3941 bp
  45% of sequence in fragments shorter than    4389 bp
  50% of sequence in fragments shorter than    4866 bp
  55% of sequence in fragments shorter than    5378 bp
  60% of sequence in fragments shorter than    5934 bp
  65% of sequence in fragments shorter than    6556 bp
  70% of sequence in fragments shorter than    7268 bp
  75% of sequence in fragments shorter than    8098 bp
  80% of sequence in fragments shorter than    9100 bp
  85% of sequence in fragments shorter than   10422 bp
  90% of sequence in fragments shorter than   12344 bp
  95% of sequence in fragments shorter than   16130 bp

# BamHI.BciVI (GGATCC+GTATCC) double digest, 1061 thousand fragments (365999+696093 sites), 2806 bp average fragment length
   5% of sequence in fragments shorter than     980 bp
  10% of sequence in fragments shorter than    1494 bp
  15% of sequence in fragments shorter than    1913 bp
  20% of sequence in fragments shorter than    2322 bp
  25% of sequence in fragments shorter than    2728 bp
  30% of sequence in fragments shorter than    3132 bp
  35% of sequence in fragments shorter than    3542 bp
  40% of sequence in fragments shorter than    3960 bp
  45% of sequence in fragments shorter than    4395 bp
  50% of sequence in fragments shorter than    4859 bp
  55% of sequence in fragments shorter than    5357 bp
  60% of sequence in fragments shorter than    5892 bp
  65% of sequence in fragments shorter than    6493 bp
  70% of sequence in fragments shorter than    7165 bp
  75% of sequence in fragments shorter than    7947 bp
  80% of sequence in fragments shorter than    8902 bp
  85% of sequence in fragments shorter than   10125 bp
  90% of sequence in fragments shorter than   11872 bp
  95% of sequence in fragments shorter than   15146 bp

# Hpy99I.NdeI (CGWCG+CATATG) double digest, 1060 thousand fragments (157917+903360 sites), 2809 bp average fragment length
   5% of sequence in fragments shorter than     967 bp
  10% of sequence in fragments shorter than    1478 bp
  15% of sequence in fragments shorter than    1957 bp
  20% of sequence in fragments shorter than    2408 bp
  25% of sequence in fragments shorter than    2845 bp
  30% of sequence in fragments shorter than    3280 bp
  35% of sequence in fragments shorter than    3726 bp
  40% of sequence in fragments shorter than    4182 bp
  45% of sequence in fragments shorter than    4637 bp
  50% of sequence in fragments shorter than    5132 bp
  55% of sequence in fragments shorter than    5668 bp
  60% of sequence in fragments shorter than    6253 bp
  65% of sequence in fragments shorter than    6904 bp
  70% of sequence in fragments shorter than    7647 bp
  75% of sequence in fragments shorter than    8525 bp
  80% of sequence in fragments shorter than    9593 bp
  85% of sequence in fragments shorter than   10982 bp
  90% of sequence in fragments shorter than   13036 bp
  95% of sequence in fragments shorter than   17048 bp

# ApaLI.MfeI (GTGCAC+CAATTG) double digest, 1060 thousand fragments (496255+564303 sites), 2811 bp average fragment length
   5% of sequence in fragments shorter than    1001 bp
  10% of sequence in fragments shorter than    1507 bp
  15% of sequence in fragments shorter than    1903 bp
  20% of sequence in fragments shorter than    2305 bp
  25% of sequence in fragments shorter than    2704 bp
  30% of sequence in fragments shorter than    3103 bp
  35% of sequence in fragments shorter than    3505 bp
  40% of sequence in fragments shorter than    3925 bp
  45% of sequence in fragments shorter than    4337 bp
  50% of sequence in fragments shorter than    4783 bp
  55% of sequence in fragments shorter than    5272 bp
  60% of sequence in fragments shorter than    5804 bp
  65% of sequence in fragments shorter than    6389 bp
  70% of sequence in fragments shorter than    7045 bp
  75% of sequence in fragments shorter than    7809 bp
  80% of sequence in fragments shorter than    8731 bp
  85% of sequence in fragments shorter than    9919 bp
  90% of sequence in fragments shorter than   11625 bp
  95% of sequence in fragments shorter than   14730 bp

# AgeI.AvaI (ACCGGT+CYCGRG) double digest, 1058 thousand fragments (53386+1005714 sites), 2814 bp average fragment length
   5% of sequence in fragments shorter than     900 bp
  10% of sequence in fragments shorter than    1491 bp
  15% of sequence in fragments shorter than    2059 bp
  20% of sequence in fragments shorter than    2633 bp
  25% of sequence in fragments shorter than    3233 bp
  30% of sequence in fragments shorter than    3869 bp
  35% of sequence in fragments shorter than    4543 bp
  40% of sequence in fragments shorter than    5276 bp
  45% of sequence in fragments shorter than    6066 bp
  50% of sequence in fragments shorter than    6942 bp
  55% of sequence in fragments shorter than    7922 bp
  60% of sequence in fragments shorter than    9033 bp
  65% of sequence in fragments shorter than   10299 bp
  70% of sequence in fragments shorter than   11760 bp
  75% of sequence in fragments shorter than   13541 bp
  80% of sequence in fragments shorter than   15751 bp
  85% of sequence in fragments shorter than   18721 bp
  90% of sequence in fragments shorter than   23201 bp
  95% of sequence in fragments shorter than   32393 bp

# AvaIII.NaeI (ATGCAT+GCCGGC) double digest, 1058 thousand fragments (933357+125692 sites), 2814 bp average fragment length
   5% of sequence in fragments shorter than    1005 bp
  10% of sequence in fragments shorter than    1533 bp
  15% of sequence in fragments shorter than    1991 bp
  20% of sequence in fragments shorter than    2412 bp
  25% of sequence in fragments shorter than    2825 bp
  30% of sequence in fragments shorter than    3247 bp
  35% of sequence in fragments shorter than    3670 bp
  40% of sequence in fragments shorter than    4118 bp
  45% of sequence in fragments shorter than    4584 bp
  50% of sequence in fragments shorter than    5075 bp
  55% of sequence in fragments shorter than    5610 bp
  60% of sequence in fragments shorter than    6193 bp
  65% of sequence in fragments shorter than    6843 bp
  70% of sequence in fragments shorter than    7581 bp
  75% of sequence in fragments shorter than    8438 bp
  80% of sequence in fragments shorter than    9487 bp
  85% of sequence in fragments shorter than   10855 bp
  90% of sequence in fragments shorter than   12816 bp
  95% of sequence in fragments shorter than   16585 bp

# SnaBI.StuI (TACGTA+AGGCCT) double digest, 1057 thousand fragments (132299+925729 sites), 2817 bp average fragment length
   5% of sequence in fragments shorter than     863 bp
  10% of sequence in fragments shorter than    1450 bp
  15% of sequence in fragments shorter than    1983 bp
  20% of sequence in fragments shorter than    2478 bp
  25% of sequence in fragments shorter than    2982 bp
  30% of sequence in fragments shorter than    3483 bp
  35% of sequence in fragments shorter than    3998 bp
  40% of sequence in fragments shorter than    4520 bp
  45% of sequence in fragments shorter than    5075 bp
  50% of sequence in fragments shorter than    5663 bp
  55% of sequence in fragments shorter than    6291 bp
  60% of sequence in fragments shorter than    6981 bp
  65% of sequence in fragments shorter than    7733 bp
  70% of sequence in fragments shorter than    8579 bp
  75% of sequence in fragments shorter than    9575 bp
  80% of sequence in fragments shorter than   10762 bp
  85% of sequence in fragments shorter than   12275 bp
  90% of sequence in fragments shorter than   14365 bp
  95% of sequence in fragments shorter than   17854 bp

# NcoI.SexAI (CCATGG+ACCWGGT) double digest, 1057 thousand fragments (759594+297900 sites), 2818 bp average fragment length
   5% of sequence in fragments shorter than     970 bp
  10% of sequence in fragments shorter than    1478 bp
  15% of sequence in fragments shorter than    1939 bp
  20% of sequence in fragments shorter than    2373 bp
  25% of sequence in fragments shorter than    2799 bp
  30% of sequence in fragments shorter than    3231 bp
  35% of sequence in fragments shorter than    3670 bp
  40% of sequence in fragments shorter than    4127 bp
  45% of sequence in fragments shorter than    4589 bp
  50% of sequence in fragments shorter than    5093 bp
  55% of sequence in fragments shorter than    5631 bp
  60% of sequence in fragments shorter than    6207 bp
  65% of sequence in fragments shorter than    6871 bp
  70% of sequence in fragments shorter than    7629 bp
  75% of sequence in fragments shorter than    8518 bp
  80% of sequence in fragments shorter than    9613 bp
  85% of sequence in fragments shorter than   11048 bp
  90% of sequence in fragments shorter than   13157 bp
  95% of sequence in fragments shorter than   17261 bp

# ApaI.AvrII (GGGCCC+CCTAGG) double digest, 1054 thousand fragments (460339+594956 sites), 2824 bp average fragment length
   5% of sequence in fragments shorter than     917 bp
  10% of sequence in fragments shorter than    1492 bp
  15% of sequence in fragments shorter than    2007 bp
  20% of sequence in fragments shorter than    2500 bp
  25% of sequence in fragments shorter than    2994 bp
  30% of sequence in fragments shorter than    3497 bp
  35% of sequence in fragments shorter than    4003 bp
  40% of sequence in fragments shorter than    4527 bp
  45% of sequence in fragments shorter than    5083 bp
  50% of sequence in fragments shorter than    5679 bp
  55% of sequence in fragments shorter than    6324 bp
  60% of sequence in fragments shorter than    7038 bp
  65% of sequence in fragments shorter than    7840 bp
  70% of sequence in fragments shorter than    8737 bp
  75% of sequence in fragments shorter than    9829 bp
  80% of sequence in fragments shorter than   11175 bp
  85% of sequence in fragments shorter than   12980 bp
  90% of sequence in fragments shorter than   15702 bp
  95% of sequence in fragments shorter than   21148 bp

# AvaIII.XhoI (ATGCAT+CTCGAG) double digest, 1054 thousand fragments (933357+121308 sites), 2826 bp average fragment length
   5% of sequence in fragments shorter than    1008 bp
  10% of sequence in fragments shorter than    1529 bp
  15% of sequence in fragments shorter than    1980 bp
  20% of sequence in fragments shorter than    2391 bp
  25% of sequence in fragments shorter than    2799 bp
  30% of sequence in fragments shorter than    3218 bp
  35% of sequence in fragments shorter than    3637 bp
  40% of sequence in fragments shorter than    4078 bp
  45% of sequence in fragments shorter than    4540 bp
  50% of sequence in fragments shorter than    5012 bp
  55% of sequence in fragments shorter than    5530 bp
  60% of sequence in fragments shorter than    6095 bp
  65% of sequence in fragments shorter than    6727 bp
  70% of sequence in fragments shorter than    7447 bp
  75% of sequence in fragments shorter than    8277 bp
  80% of sequence in fragments shorter than    9294 bp
  85% of sequence in fragments shorter than   10650 bp
  90% of sequence in fragments shorter than   12615 bp
  95% of sequence in fragments shorter than   16450 bp

# NaeI.StuI (GCCGGC+AGGCCT) double digest, 1050 thousand fragments (125692+925729 sites), 2835 bp average fragment length
   5% of sequence in fragments shorter than     843 bp
  10% of sequence in fragments shorter than    1442 bp
  15% of sequence in fragments shorter than    1990 bp
  20% of sequence in fragments shorter than    2556 bp
  25% of sequence in fragments shorter than    3114 bp
  30% of sequence in fragments shorter than    3677 bp
  35% of sequence in fragments shorter than    4256 bp
  40% of sequence in fragments shorter than    4863 bp
  45% of sequence in fragments shorter than    5505 bp
  50% of sequence in fragments shorter than    6185 bp
  55% of sequence in fragments shorter than    6925 bp
  60% of sequence in fragments shorter than    7729 bp
  65% of sequence in fragments shorter than    8615 bp
  70% of sequence in fragments shorter than    9627 bp
  75% of sequence in fragments shorter than   10812 bp
  80% of sequence in fragments shorter than   12235 bp
  85% of sequence in fragments shorter than   14030 bp
  90% of sequence in fragments shorter than   16566 bp
  95% of sequence in fragments shorter than   20966 bp

# EcoRV.SacI (GATATC+GAGCTC) double digest, 1049 thousand fragments (446473+603323 sites), 2839 bp average fragment length
   5% of sequence in fragments shorter than     982 bp
  10% of sequence in fragments shorter than    1487 bp
  15% of sequence in fragments shorter than    1938 bp
  20% of sequence in fragments shorter than    2356 bp
  25% of sequence in fragments shorter than    2769 bp
  30% of sequence in fragments shorter than    3185 bp
  35% of sequence in fragments shorter than    3599 bp
  40% of sequence in fragments shorter than    4026 bp
  45% of sequence in fragments shorter than    4469 bp
  50% of sequence in fragments shorter than    4937 bp
  55% of sequence in fragments shorter than    5442 bp
  60% of sequence in fragments shorter than    5993 bp
  65% of sequence in fragments shorter than    6599 bp
  70% of sequence in fragments shorter than    7279 bp
  75% of sequence in fragments shorter than    8079 bp
  80% of sequence in fragments shorter than    9033 bp
  85% of sequence in fragments shorter than   10269 bp
  90% of sequence in fragments shorter than   12027 bp
  95% of sequence in fragments shorter than   15219 bp

# BsaAI.BstEII (YACGTR+GGTNACC) double digest, 1049 thousand fragments (713801+335865 sites), 2840 bp average fragment length
   5% of sequence in fragments shorter than    1003 bp
  10% of sequence in fragments shorter than    1516 bp
  15% of sequence in fragments shorter than    1970 bp
  20% of sequence in fragments shorter than    2407 bp
  25% of sequence in fragments shorter than    2829 bp
  30% of sequence in fragments shorter than    3249 bp
  35% of sequence in fragments shorter than    3660 bp
  40% of sequence in fragments shorter than    4091 bp
  45% of sequence in fragments shorter than    4553 bp
  50% of sequence in fragments shorter than    5045 bp
  55% of sequence in fragments shorter than    5575 bp
  60% of sequence in fragments shorter than    6157 bp
  65% of sequence in fragments shorter than    6802 bp
  70% of sequence in fragments shorter than    7545 bp
  75% of sequence in fragments shorter than    8396 bp
  80% of sequence in fragments shorter than    9450 bp
  85% of sequence in fragments shorter than   10793 bp
  90% of sequence in fragments shorter than   12699 bp
  95% of sequence in fragments shorter than   16195 bp

# AatII.BspHI (GACGTC+TCATGA) double digest, 1048 thousand fragments (71089+978290 sites), 2840 bp average fragment length
   5% of sequence in fragments shorter than    1007 bp
  10% of sequence in fragments shorter than    1527 bp
  15% of sequence in fragments shorter than    1970 bp
  20% of sequence in fragments shorter than    2362 bp
  25% of sequence in fragments shorter than    2765 bp
  30% of sequence in fragments shorter than    3165 bp
  35% of sequence in fragments shorter than    3575 bp
  40% of sequence in fragments shorter than    4000 bp
  45% of sequence in fragments shorter than    4441 bp
  50% of sequence in fragments shorter than    4902 bp
  55% of sequence in fragments shorter than    5402 bp
  60% of sequence in fragments shorter than    5940 bp
  65% of sequence in fragments shorter than    6547 bp
  70% of sequence in fragments shorter than    7227 bp
  75% of sequence in fragments shorter than    8032 bp
  80% of sequence in fragments shorter than    9013 bp
  85% of sequence in fragments shorter than   10290 bp
  90% of sequence in fragments shorter than   12168 bp
  95% of sequence in fragments shorter than   15845 bp

# AvaI.PmeI (CYCGRG+GTTTAAAC) double digest, 1047 thousand fragments (1005714+42094 sites), 2845 bp average fragment length
   5% of sequence in fragments shorter than     918 bp
  10% of sequence in fragments shorter than    1512 bp
  15% of sequence in fragments shorter than    2077 bp
  20% of sequence in fragments shorter than    2648 bp
  25% of sequence in fragments shorter than    3239 bp
  30% of sequence in fragments shorter than    3868 bp
  35% of sequence in fragments shorter than    4536 bp
  40% of sequence in fragments shorter than    5257 bp
  45% of sequence in fragments shorter than    6027 bp
  50% of sequence in fragments shorter than    6882 bp
  55% of sequence in fragments shorter than    7835 bp
  60% of sequence in fragments shorter than    8906 bp
  65% of sequence in fragments shorter than   10129 bp
  70% of sequence in fragments shorter than   11541 bp
  75% of sequence in fragments shorter than   13240 bp
  80% of sequence in fragments shorter than   15356 bp
  85% of sequence in fragments shorter than   18208 bp
  90% of sequence in fragments shorter than   22485 bp
  95% of sequence in fragments shorter than   31218 bp

# KpnI.NcoI (GGTACC+CCATGG) double digest, 1047 thousand fragments (287974+759594 sites), 2845 bp average fragment length
   5% of sequence in fragments shorter than    1005 bp
  10% of sequence in fragments shorter than    1451 bp
  15% of sequence in fragments shorter than    1905 bp
  20% of sequence in fragments shorter than    2341 bp
  25% of sequence in fragments shorter than    2780 bp
  30% of sequence in fragments shorter than    3219 bp
  35% of sequence in fragments shorter than    3661 bp
  40% of sequence in fragments shorter than    4129 bp
  45% of sequence in fragments shorter than    4624 bp
  50% of sequence in fragments shorter than    5150 bp
  55% of sequence in fragments shorter than    5715 bp
  60% of sequence in fragments shorter than    6336 bp
  65% of sequence in fragments shorter than    7027 bp
  70% of sequence in fragments shorter than    7821 bp
  75% of sequence in fragments shorter than    8750 bp
  80% of sequence in fragments shorter than    9885 bp
  85% of sequence in fragments shorter than   11384 bp
  90% of sequence in fragments shorter than   13551 bp
  95% of sequence in fragments shorter than   17878 bp

# ApaLI.SphI (GTGCAC+GCATGC) double digest, 1046 thousand fragments (496255+550952 sites), 2846 bp average fragment length
   5% of sequence in fragments shorter than    1000 bp
  10% of sequence in fragments shorter than    1512 bp
  15% of sequence in fragments shorter than    1960 bp
  20% of sequence in fragments shorter than    2381 bp
  25% of sequence in fragments shorter than    2796 bp
  30% of sequence in fragments shorter than    3214 bp
  35% of sequence in fragments shorter than    3638 bp
  40% of sequence in fragments shorter than    4084 bp
  45% of sequence in fragments shorter than    4552 bp
  50% of sequence in fragments shorter than    5041 bp
  55% of sequence in fragments shorter than    5563 bp
  60% of sequence in fragments shorter than    6125 bp
  65% of sequence in fragments shorter than    6764 bp
  70% of sequence in fragments shorter than    7494 bp
  75% of sequence in fragments shorter than    8349 bp
  80% of sequence in fragments shorter than    9401 bp
  85% of sequence in fragments shorter than   10784 bp
  90% of sequence in fragments shorter than   12781 bp
  95% of sequence in fragments shorter than   16664 bp

# StuI.XhoI (AGGCCT+CTCGAG) double digest, 1046 thousand fragments (925729+121308 sites), 2847 bp average fragment length
   5% of sequence in fragments shorter than     853 bp
  10% of sequence in fragments shorter than    1442 bp
  15% of sequence in fragments shorter than    1988 bp
  20% of sequence in fragments shorter than    2519 bp
  25% of sequence in fragments shorter than    3052 bp
  30% of sequence in fragments shorter than    3590 bp
  35% of sequence in fragments shorter than    4140 bp
  40% of sequence in fragments shorter than    4712 bp
  45% of sequence in fragments shorter than    5318 bp
  50% of sequence in fragments shorter than    5957 bp
  55% of sequence in fragments shorter than    6654 bp
  60% of sequence in fragments shorter than    7415 bp
  65% of sequence in fragments shorter than    8247 bp
  70% of sequence in fragments shorter than    9192 bp
  75% of sequence in fragments shorter than   10309 bp
  80% of sequence in fragments shorter than   11635 bp
  85% of sequence in fragments shorter than   13335 bp
  90% of sequence in fragments shorter than   15690 bp
  95% of sequence in fragments shorter than   19831 bp

# NcoI.NheI (CCATGG+GCTAGC) double digest, 1046 thousand fragments (759594+287384 sites), 2847 bp average fragment length
   5% of sequence in fragments shorter than     964 bp
  10% of sequence in fragments shorter than    1493 bp
  15% of sequence in fragments shorter than    1912 bp
  20% of sequence in fragments shorter than    2350 bp
  25% of sequence in fragments shorter than    2762 bp
  30% of sequence in fragments shorter than    3188 bp
  35% of sequence in fragments shorter than    3637 bp
  40% of sequence in fragments shorter than    4098 bp
  45% of sequence in fragments shorter than    4584 bp
  50% of sequence in fragments shorter than    5102 bp
  55% of sequence in fragments shorter than    5657 bp
  60% of sequence in fragments shorter than    6272 bp
  65% of sequence in fragments shorter than    6953 bp
  70% of sequence in fragments shorter than    7724 bp
  75% of sequence in fragments shorter than    8641 bp
  80% of sequence in fragments shorter than    9744 bp
  85% of sequence in fragments shorter than   11182 bp
  90% of sequence in fragments shorter than   13270 bp
  95% of sequence in fragments shorter than   17257 bp

# BspHI.SacII (TCATGA+CCGCGG) double digest, 1044 thousand fragments (978290+66901 sites), 2852 bp average fragment length
   5% of sequence in fragments shorter than    1015 bp
  10% of sequence in fragments shorter than    1550 bp
  15% of sequence in fragments shorter than    1999 bp
  20% of sequence in fragments shorter than    2403 bp
  25% of sequence in fragments shorter than    2815 bp
  30% of sequence in fragments shorter than    3221 bp
  35% of sequence in fragments shorter than    3639 bp
  40% of sequence in fragments shorter than    4072 bp
  45% of sequence in fragments shorter than    4517 bp
  50% of sequence in fragments shorter than    4988 bp
  55% of sequence in fragments shorter than    5495 bp
  60% of sequence in fragments shorter than    6040 bp
  65% of sequence in fragments shorter than    6648 bp
  70% of sequence in fragments shorter than    7343 bp
  75% of sequence in fragments shorter than    8153 bp
  80% of sequence in fragments shorter than    9136 bp
  85% of sequence in fragments shorter than   10419 bp
  90% of sequence in fragments shorter than   12309 bp
  95% of sequence in fragments shorter than   15924 bp

# AflII.SpeI (CTTAAG+ACTAGT) double digest, 1043 thousand fragments (643284+400286 sites), 2856 bp average fragment length
   5% of sequence in fragments shorter than    1001 bp
  10% of sequence in fragments shorter than    1471 bp
  15% of sequence in fragments shorter than    1914 bp
  20% of sequence in fragments shorter than    2327 bp
  25% of sequence in fragments shorter than    2736 bp
  30% of sequence in fragments shorter than    3144 bp
  35% of sequence in fragments shorter than    3564 bp
  40% of sequence in fragments shorter than    3991 bp
  45% of sequence in fragments shorter than    4448 bp
  50% of sequence in fragments shorter than    4930 bp
  55% of sequence in fragments shorter than    5454 bp
  60% of sequence in fragments shorter than    6030 bp
  65% of sequence in fragments shorter than    6672 bp
  70% of sequence in fragments shorter than    7422 bp
  75% of sequence in fragments shorter than    8295 bp
  80% of sequence in fragments shorter than    9419 bp
  85% of sequence in fragments shorter than   10927 bp
  90% of sequence in fragments shorter than   13230 bp
  95% of sequence in fragments shorter than   18442 bp

# AvrII.EcoRV (CCTAGG+GATATC) double digest, 1040 thousand fragments (594956+446473 sites), 2862 bp average fragment length
   5% of sequence in fragments shorter than     994 bp
  10% of sequence in fragments shorter than    1492 bp
  15% of sequence in fragments shorter than    1936 bp
  20% of sequence in fragments shorter than    2366 bp
  25% of sequence in fragments shorter than    2775 bp
  30% of sequence in fragments shorter than    3187 bp
  35% of sequence in fragments shorter than    3602 bp
  40% of sequence in fragments shorter than    4032 bp
  45% of sequence in fragments shorter than    4479 bp
  50% of sequence in fragments shorter than    4954 bp
  55% of sequence in fragments shorter than    5463 bp
  60% of sequence in fragments shorter than    6011 bp
  65% of sequence in fragments shorter than    6621 bp
  70% of sequence in fragments shorter than    7310 bp
  75% of sequence in fragments shorter than    8108 bp
  80% of sequence in fragments shorter than    9069 bp
  85% of sequence in fragments shorter than   10295 bp
  90% of sequence in fragments shorter than   12084 bp
  95% of sequence in fragments shorter than   15344 bp

# BceAI.BstEII (ACGGC+GGTNACC) double digest, 1040 thousand fragments (705178+335865 sites), 2863 bp average fragment length
   5% of sequence in fragments shorter than     937 bp
  10% of sequence in fragments shorter than    1505 bp
  15% of sequence in fragments shorter than    2028 bp
  20% of sequence in fragments shorter than    2541 bp
  25% of sequence in fragments shorter than    3055 bp
  30% of sequence in fragments shorter than    3572 bp
  35% of sequence in fragments shorter than    4115 bp
  40% of sequence in fragments shorter than    4681 bp
  45% of sequence in fragments shorter than    5273 bp
  50% of sequence in fragments shorter than    5909 bp
  55% of sequence in fragments shorter than    6597 bp
  60% of sequence in fragments shorter than    7361 bp
  65% of sequence in fragments shorter than    8218 bp
  70% of sequence in fragments shorter than    9189 bp
  75% of sequence in fragments shorter than   10354 bp
  80% of sequence in fragments shorter than   11783 bp
  85% of sequence in fragments shorter than   13660 bp
  90% of sequence in fragments shorter than   16349 bp
  95% of sequence in fragments shorter than   21419 bp

# ApaLI.ScaI (GTGCAC+AGTACT) double digest, 1039 thousand fragments (496255+543793 sites), 2866 bp average fragment length
   5% of sequence in fragments shorter than    1023 bp
  10% of sequence in fragments shorter than    1528 bp
  15% of sequence in fragments shorter than    1966 bp
  20% of sequence in fragments shorter than    2377 bp
  25% of sequence in fragments shorter than    2775 bp
  30% of sequence in fragments shorter than    3176 bp
  35% of sequence in fragments shorter than    3585 bp
  40% of sequence in fragments shorter than    4007 bp
  45% of sequence in fragments shorter than    4450 bp
  50% of sequence in fragments shorter than    4916 bp
  55% of sequence in fragments shorter than    5422 bp
  60% of sequence in fragments shorter than    5952 bp
  65% of sequence in fragments shorter than    6544 bp
  70% of sequence in fragments shorter than    7222 bp
  75% of sequence in fragments shorter than    8002 bp
  80% of sequence in fragments shorter than    8955 bp
  85% of sequence in fragments shorter than   10201 bp
  90% of sequence in fragments shorter than   11962 bp
  95% of sequence in fragments shorter than   15423 bp

# BspHI.PspXI (TCATGA+VCTCGAGB) double digest, 1037 thousand fragments (978290+59193 sites), 2873 bp average fragment length
   5% of sequence in fragments shorter than    1016 bp
  10% of sequence in fragments shorter than    1542 bp
  15% of sequence in fragments shorter than    1987 bp
  20% of sequence in fragments shorter than    2384 bp
  25% of sequence in fragments shorter than    2791 bp
  30% of sequence in fragments shorter than    3191 bp
  35% of sequence in fragments shorter than    3605 bp
  40% of sequence in fragments shorter than    4033 bp
  45% of sequence in fragments shorter than    4474 bp
  50% of sequence in fragments shorter than    4941 bp
  55% of sequence in fragments shorter than    5447 bp
  60% of sequence in fragments shorter than    5997 bp
  65% of sequence in fragments shorter than    6606 bp
  70% of sequence in fragments shorter than    7297 bp
  75% of sequence in fragments shorter than    8106 bp
  80% of sequence in fragments shorter than    9098 bp
  85% of sequence in fragments shorter than   10384 bp
  90% of sequence in fragments shorter than   12269 bp
  95% of sequence in fragments shorter than   16003 bp

# AvaI.SalI (CYCGRG+GTCGAC) double digest, 1036 thousand fragments (1005714+31396 sites), 2874 bp average fragment length
   5% of sequence in fragments shorter than     920 bp
  10% of sequence in fragments shorter than    1525 bp
  15% of sequence in fragments shorter than    2105 bp
  20% of sequence in fragments shorter than    2693 bp
  25% of sequence in fragments shorter than    3308 bp
  30% of sequence in fragments shorter than    3966 bp
  35% of sequence in fragments shorter than    4662 bp
  40% of sequence in fragments shorter than    5407 bp
  45% of sequence in fragments shorter than    6225 bp
  50% of sequence in fragments shorter than    7127 bp
  55% of sequence in fragments shorter than    8145 bp
  60% of sequence in fragments shorter than    9291 bp
  65% of sequence in fragments shorter than   10584 bp
  70% of sequence in fragments shorter than   12101 bp
  75% of sequence in fragments shorter than   13940 bp
  80% of sequence in fragments shorter than   16248 bp
  85% of sequence in fragments shorter than   19280 bp
  90% of sequence in fragments shorter than   23996 bp
  95% of sequence in fragments shorter than   33525 bp

# BclI.SexAI (TGATCA+ACCWGGT) double digest, 1036 thousand fragments (738785+297900 sites), 2875 bp average fragment length
   5% of sequence in fragments shorter than    1011 bp
  10% of sequence in fragments shorter than    1526 bp
  15% of sequence in fragments shorter than    1962 bp
  20% of sequence in fragments shorter than    2378 bp
  25% of sequence in fragments shorter than    2767 bp
  30% of sequence in fragments shorter than    3165 bp
  35% of sequence in fragments shorter than    3570 bp
  40% of sequence in fragments shorter than    3982 bp
  45% of sequence in fragments shorter than    4413 bp
  50% of sequence in fragments shorter than    4876 bp
  55% of sequence in fragments shorter than    5372 bp
  60% of sequence in fragments shorter than    5911 bp
  65% of sequence in fragments shorter than    6506 bp
  70% of sequence in fragments shorter than    7183 bp
  75% of sequence in fragments shorter than    7967 bp
  80% of sequence in fragments shorter than    8942 bp
  85% of sequence in fragments shorter than   10210 bp
  90% of sequence in fragments shorter than   12060 bp
  95% of sequence in fragments shorter than   15696 bp

# NdeI.SnaBI (CATATG+TACGTA) double digest, 1035 thousand fragments (903360+132299 sites), 2878 bp average fragment length
   5% of sequence in fragments shorter than     976 bp
  10% of sequence in fragments shorter than    1479 bp
  15% of sequence in fragments shorter than    1967 bp
  20% of sequence in fragments shorter than    2432 bp
  25% of sequence in fragments shorter than    2881 bp
  30% of sequence in fragments shorter than    3336 bp
  35% of sequence in fragments shorter than    3808 bp
  40% of sequence in fragments shorter than    4296 bp
  45% of sequence in fragments shorter than    4803 bp
  50% of sequence in fragments shorter than    5337 bp
  55% of sequence in fragments shorter than    5922 bp
  60% of sequence in fragments shorter than    6566 bp
  65% of sequence in fragments shorter than    7302 bp
  70% of sequence in fragments shorter than    8165 bp
  75% of sequence in fragments shorter than    9204 bp
  80% of sequence in fragments shorter than   10499 bp
  85% of sequence in fragments shorter than   12269 bp
  90% of sequence in fragments shorter than   15080 bp
  95% of sequence in fragments shorter than   21390 bp

# AclI.HindIII (AACGTT+AAGCTT) double digest, 1032 thousand fragments (172706+860365 sites), 2885 bp average fragment length
   5% of sequence in fragments shorter than    1020 bp
  10% of sequence in fragments shorter than    1517 bp
  15% of sequence in fragments shorter than    1938 bp
  20% of sequence in fragments shorter than    2365 bp
  25% of sequence in fragments shorter than    2782 bp
  30% of sequence in fragments shorter than    3197 bp
  35% of sequence in fragments shorter than    3626 bp
  40% of sequence in fragments shorter than    4061 bp
  45% of sequence in fragments shorter than    4516 bp
  50% of sequence in fragments shorter than    5003 bp
  55% of sequence in fragments shorter than    5526 bp
  60% of sequence in fragments shorter than    6100 bp
  65% of sequence in fragments shorter than    6733 bp
  70% of sequence in fragments shorter than    7453 bp
  75% of sequence in fragments shorter than    8313 bp
  80% of sequence in fragments shorter than    9334 bp
  85% of sequence in fragments shorter than   10678 bp
  90% of sequence in fragments shorter than   12616 bp
  95% of sequence in fragments shorter than   16244 bp

# AflII.HpaI (CTTAAG+GTTAAC) double digest, 1032 thousand fragments (643284+389418 sites), 2886 bp average fragment length
   5% of sequence in fragments shorter than    1009 bp
  10% of sequence in fragments shorter than    1529 bp
  15% of sequence in fragments shorter than    1960 bp
  20% of sequence in fragments shorter than    2378 bp
  25% of sequence in fragments shorter than    2786 bp
  30% of sequence in fragments shorter than    3195 bp
  35% of sequence in fragments shorter than    3612 bp
  40% of sequence in fragments shorter than    4041 bp
  45% of sequence in fragments shorter than    4499 bp
  50% of sequence in fragments shorter than    4978 bp
  55% of sequence in fragments shorter than    5502 bp
  60% of sequence in fragments shorter than    6074 bp
  65% of sequence in fragments shorter than    6724 bp
  70% of sequence in fragments shorter than    7466 bp
  75% of sequence in fragments shorter than    8332 bp
  80% of sequence in fragments shorter than    9445 bp
  85% of sequence in fragments shorter than   10914 bp
  90% of sequence in fragments shorter than   13225 bp
  95% of sequence in fragments shorter than   18319 bp

# ApaI.HgaI (GGGCCC+GACGC) double digest, 1031 thousand fragments (460339+571889 sites), 2888 bp average fragment length
   5% of sequence in fragments shorter than     910 bp
  10% of sequence in fragments shorter than    1578 bp
  15% of sequence in fragments shorter than    2223 bp
  20% of sequence in fragments shorter than    2859 bp
  25% of sequence in fragments shorter than    3514 bp
  30% of sequence in fragments shorter than    4198 bp
  35% of sequence in fragments shorter than    4932 bp
  40% of sequence in fragments shorter than    5699 bp
  45% of sequence in fragments shorter than    6521 bp
  50% of sequence in fragments shorter than    7403 bp
  55% of sequence in fragments shorter than    8367 bp
  60% of sequence in fragments shorter than    9427 bp
  65% of sequence in fragments shorter than   10647 bp
  70% of sequence in fragments shorter than   12036 bp
  75% of sequence in fragments shorter than   13694 bp
  80% of sequence in fragments shorter than   15727 bp
  85% of sequence in fragments shorter than   18349 bp
  90% of sequence in fragments shorter than   22289 bp
  95% of sequence in fragments shorter than   29787 bp

# BciVI.BstEII (GTATCC+GGTNACC) double digest, 1031 thousand fragments (696093+335865 sites), 2888 bp average fragment length
   5% of sequence in fragments shorter than    1018 bp
  10% of sequence in fragments shorter than    1548 bp
  15% of sequence in fragments shorter than    1978 bp
  20% of sequence in fragments shorter than    2391 bp
  25% of sequence in fragments shorter than    2781 bp
  30% of sequence in fragments shorter than    3190 bp
  35% of sequence in fragments shorter than    3602 bp
  40% of sequence in fragments shorter than    4029 bp
  45% of sequence in fragments shorter than    4471 bp
  50% of sequence in fragments shorter than    4945 bp
  55% of sequence in fragments shorter than    5457 bp
  60% of sequence in fragments shorter than    6009 bp
  65% of sequence in fragments shorter than    6622 bp
  70% of sequence in fragments shorter than    7316 bp
  75% of sequence in fragments shorter than    8118 bp
  80% of sequence in fragments shorter than    9097 bp
  85% of sequence in fragments shorter than   10361 bp
  90% of sequence in fragments shorter than   12182 bp
  95% of sequence in fragments shorter than   15683 bp

# FnuDII.SexAI (CGCG+ACCWGGT) double digest, 1031 thousand fragments (733940+297900 sites), 2889 bp average fragment length
   5% of sequence in fragments shorter than    1163 bp
  10% of sequence in fragments shorter than    1962 bp
  15% of sequence in fragments shorter than    2676 bp
  20% of sequence in fragments shorter than    3372 bp
  25% of sequence in fragments shorter than    4074 bp
  30% of sequence in fragments shorter than    4806 bp
  35% of sequence in fragments shorter than    5563 bp
  40% of sequence in fragments shorter than    6368 bp
  45% of sequence in fragments shorter than    7216 bp
  50% of sequence in fragments shorter than    8137 bp
  55% of sequence in fragments shorter than    9150 bp
  60% of sequence in fragments shorter than   10272 bp
  65% of sequence in fragments shorter than   11521 bp
  70% of sequence in fragments shorter than   12986 bp
  75% of sequence in fragments shorter than   14719 bp
  80% of sequence in fragments shorter than   16906 bp
  85% of sequence in fragments shorter than   19752 bp
  90% of sequence in fragments shorter than   23870 bp
  95% of sequence in fragments shorter than   31955 bp

# AgeI.BspHI (ACCGGT+TCATGA) double digest, 1031 thousand fragments (53386+978290 sites), 2889 bp average fragment length
   5% of sequence in fragments shorter than    1023 bp
  10% of sequence in fragments shorter than    1553 bp
  15% of sequence in fragments shorter than    1998 bp
  20% of sequence in fragments shorter than    2391 bp
  25% of sequence in fragments shorter than    2792 bp
  30% of sequence in fragments shorter than    3198 bp
  35% of sequence in fragments shorter than    3615 bp
  40% of sequence in fragments shorter than    4048 bp
  45% of sequence in fragments shorter than    4496 bp
  50% of sequence in fragments shorter than    4971 bp
  55% of sequence in fragments shorter than    5483 bp
  60% of sequence in fragments shorter than    6041 bp
  65% of sequence in fragments shorter than    6655 bp
  70% of sequence in fragments shorter than    7365 bp
  75% of sequence in fragments shorter than    8200 bp
  80% of sequence in fragments shorter than    9214 bp
  85% of sequence in fragments shorter than   10549 bp
  90% of sequence in fragments shorter than   12511 bp
  95% of sequence in fragments shorter than   16450 bp

# ApaI.EciI (GGGCCC+GGCGGA) double digest, 1031 thousand fragments (460339+571273 sites), 2889 bp average fragment length
   5% of sequence in fragments shorter than     891 bp
  10% of sequence in fragments shorter than    1488 bp
  15% of sequence in fragments shorter than    2060 bp
  20% of sequence in fragments shorter than    2632 bp
  25% of sequence in fragments shorter than    3229 bp
  30% of sequence in fragments shorter than    3869 bp
  35% of sequence in fragments shorter than    4560 bp
  40% of sequence in fragments shorter than    5312 bp
  45% of sequence in fragments shorter than    6126 bp
  50% of sequence in fragments shorter than    7034 bp
  55% of sequence in fragments shorter than    8044 bp
  60% of sequence in fragments shorter than    9170 bp
  65% of sequence in fragments shorter than   10478 bp
  70% of sequence in fragments shorter than   11977 bp
  75% of sequence in fragments shorter than   13829 bp
  80% of sequence in fragments shorter than   16177 bp
  85% of sequence in fragments shorter than   19238 bp
  90% of sequence in fragments shorter than   23823 bp
  95% of sequence in fragments shorter than   33530 bp

# HaeII.SpeI (RGCGCY+ACTAGT) double digest, 1029 thousand fragments (630101+400286 sites), 2893 bp average fragment length
   5% of sequence in fragments shorter than     932 bp
  10% of sequence in fragments shorter than    1555 bp
  15% of sequence in fragments shorter than    2097 bp
  20% of sequence in fragments shorter than    2624 bp
  25% of sequence in fragments shorter than    3141 bp
  30% of sequence in fragments shorter than    3636 bp
  35% of sequence in fragments shorter than    4157 bp
  40% of sequence in fragments shorter than    4674 bp
  45% of sequence in fragments shorter than    5214 bp
  50% of sequence in fragments shorter than    5802 bp
  55% of sequence in fragments shorter than    6437 bp
  60% of sequence in fragments shorter than    7129 bp
  65% of sequence in fragments shorter than    7892 bp
  70% of sequence in fragments shorter than    8759 bp
  75% of sequence in fragments shorter than    9764 bp
  80% of sequence in fragments shorter than   10996 bp
  85% of sequence in fragments shorter than   12519 bp
  90% of sequence in fragments shorter than   14675 bp
  95% of sequence in fragments shorter than   18322 bp

# NaeI.NdeI (GCCGGC+CATATG) double digest, 1028 thousand fragments (125692+903360 sites), 2896 bp average fragment length
   5% of sequence in fragments shorter than     997 bp
  10% of sequence in fragments shorter than    1521 bp
  15% of sequence in fragments shorter than    2012 bp
  20% of sequence in fragments shorter than    2474 bp
  25% of sequence in fragments shorter than    2922 bp
  30% of sequence in fragments shorter than    3369 bp
  35% of sequence in fragments shorter than    3831 bp
  40% of sequence in fragments shorter than    4299 bp
  45% of sequence in fragments shorter than    4785 bp
  50% of sequence in fragments shorter than    5287 bp
  55% of sequence in fragments shorter than    5837 bp
  60% of sequence in fragments shorter than    6436 bp
  65% of sequence in fragments shorter than    7101 bp
  70% of sequence in fragments shorter than    7865 bp
  75% of sequence in fragments shorter than    8764 bp
  80% of sequence in fragments shorter than    9859 bp
  85% of sequence in fragments shorter than   11280 bp
  90% of sequence in fragments shorter than   13395 bp
  95% of sequence in fragments shorter than   17616 bp

# AvaI.MluI (CYCGRG+ACGCGT) double digest, 1026 thousand fragments (1005714+21415 sites), 2902 bp average fragment length
   5% of sequence in fragments shorter than     927 bp
  10% of sequence in fragments shorter than    1541 bp
  15% of sequence in fragments shorter than    2131 bp
  20% of sequence in fragments shorter than    2733 bp
  25% of sequence in fragments shorter than    3363 bp
  30% of sequence in fragments shorter than    4036 bp
  35% of sequence in fragments shorter than    4752 bp
  40% of sequence in fragments shorter than    5524 bp
  45% of sequence in fragments shorter than    6370 bp
  50% of sequence in fragments shorter than    7310 bp
  55% of sequence in fragments shorter than    8348 bp
  60% of sequence in fragments shorter than    9541 bp
  65% of sequence in fragments shorter than   10896 bp
  70% of sequence in fragments shorter than   12477 bp
  75% of sequence in fragments shorter than   14404 bp
  80% of sequence in fragments shorter than   16767 bp
  85% of sequence in fragments shorter than   19926 bp
  90% of sequence in fragments shorter than   24809 bp
  95% of sequence in fragments shorter than   34849 bp

# BclI.KpnI (TGATCA+GGTACC) double digest, 1026 thousand fragments (738785+287974 sites), 2903 bp average fragment length
   5% of sequence in fragments shorter than    1000 bp
  10% of sequence in fragments shorter than    1527 bp
  15% of sequence in fragments shorter than    1948 bp
  20% of sequence in fragments shorter than    2373 bp
  25% of sequence in fragments shorter than    2785 bp
  30% of sequence in fragments shorter than    3200 bp
  35% of sequence in fragments shorter than    3612 bp
  40% of sequence in fragments shorter than    4041 bp
  45% of sequence in fragments shorter than    4494 bp
  50% of sequence in fragments shorter than    4971 bp
  55% of sequence in fragments shorter than    5480 bp
  60% of sequence in fragments shorter than    6039 bp
  65% of sequence in fragments shorter than    6660 bp
  70% of sequence in fragments shorter than    7363 bp
  75% of sequence in fragments shorter than    8189 bp
  80% of sequence in fragments shorter than    9196 bp
  85% of sequence in fragments shorter than   10537 bp
  90% of sequence in fragments shorter than   12453 bp
  95% of sequence in fragments shorter than   16354 bp

# BclI.NheI (TGATCA+GCTAGC) double digest, 1025 thousand fragments (738785+287384 sites), 2905 bp average fragment length
   5% of sequence in fragments shorter than    1014 bp
  10% of sequence in fragments shorter than    1521 bp
  15% of sequence in fragments shorter than    1957 bp
  20% of sequence in fragments shorter than    2378 bp
  25% of sequence in fragments shorter than    2784 bp
  30% of sequence in fragments shorter than    3204 bp
  35% of sequence in fragments shorter than    3630 bp
  40% of sequence in fragments shorter than    4069 bp
  45% of sequence in fragments shorter than    4525 bp
  50% of sequence in fragments shorter than    5007 bp
  55% of sequence in fragments shorter than    5526 bp
  60% of sequence in fragments shorter than    6096 bp
  65% of sequence in fragments shorter than    6724 bp
  70% of sequence in fragments shorter than    7432 bp
  75% of sequence in fragments shorter than    8265 bp
  80% of sequence in fragments shorter than    9273 bp
  85% of sequence in fragments shorter than   10604 bp
  90% of sequence in fragments shorter than   12516 bp
  95% of sequence in fragments shorter than   16443 bp

# NdeI.XhoI (CATATG+CTCGAG) double digest, 1024 thousand fragments (903360+121308 sites), 2909 bp average fragment length
   5% of sequence in fragments shorter than     999 bp
  10% of sequence in fragments shorter than    1514 bp
  15% of sequence in fragments shorter than    2000 bp
  20% of sequence in fragments shorter than    2450 bp
  25% of sequence in fragments shorter than    2892 bp
  30% of sequence in fragments shorter than    3306 bp
  35% of sequence in fragments shorter than    3749 bp
  40% of sequence in fragments shorter than    4218 bp
  45% of sequence in fragments shorter than    4706 bp
  50% of sequence in fragments shorter than    5219 bp
  55% of sequence in fragments shorter than    5778 bp
  60% of sequence in fragments shorter than    6381 bp
  65% of sequence in fragments shorter than    7061 bp
  70% of sequence in fragments shorter than    7839 bp
  75% of sequence in fragments shorter than    8763 bp
  80% of sequence in fragments shorter than    9911 bp
  85% of sequence in fragments shorter than   11400 bp
  90% of sequence in fragments shorter than   13582 bp
  95% of sequence in fragments shorter than   18064 bp

# ApaI.MfeI (GGGCCC+CAATTG) double digest, 1024 thousand fragments (460339+564303 sites), 2909 bp average fragment length
   5% of sequence in fragments shorter than     992 bp
  10% of sequence in fragments shorter than    1546 bp
  15% of sequence in fragments shorter than    2029 bp
  20% of sequence in fragments shorter than    2479 bp
  25% of sequence in fragments shorter than    2924 bp
  30% of sequence in fragments shorter than    3373 bp
  35% of sequence in fragments shorter than    3838 bp
  40% of sequence in fragments shorter than    4315 bp
  45% of sequence in fragments shorter than    4803 bp
  50% of sequence in fragments shorter than    5327 bp
  55% of sequence in fragments shorter than    5894 bp
  60% of sequence in fragments shorter than    6518 bp
  65% of sequence in fragments shorter than    7218 bp
  70% of sequence in fragments shorter than    7994 bp
  75% of sequence in fragments shorter than    8915 bp
  80% of sequence in fragments shorter than   10043 bp
  85% of sequence in fragments shorter than   11503 bp
  90% of sequence in fragments shorter than   13603 bp
  95% of sequence in fragments shorter than   17568 bp

# AclI.XbaI (AACGTT+TCTAGA) double digest, 1023 thousand fragments (172706+850998 sites), 2912 bp average fragment length
   5% of sequence in fragments shorter than     982 bp
  10% of sequence in fragments shorter than    1497 bp
  15% of sequence in fragments shorter than    1954 bp
  20% of sequence in fragments shorter than    2381 bp
  25% of sequence in fragments shorter than    2820 bp
  30% of sequence in fragments shorter than    3254 bp
  35% of sequence in fragments shorter than    3694 bp
  40% of sequence in fragments shorter than    4152 bp
  45% of sequence in fragments shorter than    4625 bp
  50% of sequence in fragments shorter than    5127 bp
  55% of sequence in fragments shorter than    5661 bp
  60% of sequence in fragments shorter than    6251 bp
  65% of sequence in fragments shorter than    6907 bp
  70% of sequence in fragments shorter than    7648 bp
  75% of sequence in fragments shorter than    8515 bp
  80% of sequence in fragments shorter than    9564 bp
  85% of sequence in fragments shorter than   10932 bp
  90% of sequence in fragments shorter than   12866 bp
  95% of sequence in fragments shorter than   16269 bp

# FnuDII.KpnI (CGCG+GGTACC) double digest, 1021 thousand fragments (733940+287974 sites), 2917 bp average fragment length
   5% of sequence in fragments shorter than    1216 bp
  10% of sequence in fragments shorter than    1950 bp
  15% of sequence in fragments shorter than    2688 bp
  20% of sequence in fragments shorter than    3379 bp
  25% of sequence in fragments shorter than    4072 bp
  30% of sequence in fragments shorter than    4797 bp
  35% of sequence in fragments shorter than    5541 bp
  40% of sequence in fragments shorter than    6326 bp
  45% of sequence in fragments shorter than    7157 bp
  50% of sequence in fragments shorter than    8048 bp
  55% of sequence in fragments shorter than    9019 bp
  60% of sequence in fragments shorter than   10097 bp
  65% of sequence in fragments shorter than   11303 bp
  70% of sequence in fragments shorter than   12688 bp
  75% of sequence in fragments shorter than   14365 bp
  80% of sequence in fragments shorter than   16403 bp
  85% of sequence in fragments shorter than   19059 bp
  90% of sequence in fragments shorter than   22946 bp
  95% of sequence in fragments shorter than   30591 bp

# FnuDII.NheI (CGCG+GCTAGC) double digest, 1020 thousand fragments (733940+287384 sites), 2918 bp average fragment length
   5% of sequence in fragments shorter than    1238 bp
  10% of sequence in fragments shorter than    1987 bp
  15% of sequence in fragments shorter than    2691 bp
  20% of sequence in fragments shorter than    3367 bp
  25% of sequence in fragments shorter than    4070 bp
  30% of sequence in fragments shorter than    4799 bp
  35% of sequence in fragments shorter than    5541 bp
  40% of sequence in fragments shorter than    6320 bp
  45% of sequence in fragments shorter than    7151 bp
  50% of sequence in fragments shorter than    8037 bp
  55% of sequence in fragments shorter than    8997 bp
  60% of sequence in fragments shorter than   10066 bp
  65% of sequence in fragments shorter than   11244 bp
  70% of sequence in fragments shorter than   12588 bp
  75% of sequence in fragments shorter than   14201 bp
  80% of sequence in fragments shorter than   16206 bp
  85% of sequence in fragments shorter than   18810 bp
  90% of sequence in fragments shorter than   22537 bp
  95% of sequence in fragments shorter than   29613 bp

# AvaI.SgrAI (CYCGRG+CRCCGGYG) double digest, 1020 thousand fragments (1005714+15270 sites), 2919 bp average fragment length
   5% of sequence in fragments shorter than     936 bp
  10% of sequence in fragments shorter than    1557 bp
  15% of sequence in fragments shorter than    2153 bp
  20% of sequence in fragments shorter than    2761 bp
  25% of sequence in fragments shorter than    3400 bp
  30% of sequence in fragments shorter than    4079 bp
  35% of sequence in fragments shorter than    4804 bp
  40% of sequence in fragments shorter than    5582 bp
  45% of sequence in fragments shorter than    6438 bp
  50% of sequence in fragments shorter than    7389 bp
  55% of sequence in fragments shorter than    8440 bp
  60% of sequence in fragments shorter than    9645 bp
  65% of sequence in fragments shorter than   11003 bp
  70% of sequence in fragments shorter than   12615 bp
  75% of sequence in fragments shorter than   14564 bp
  80% of sequence in fragments shorter than   16966 bp
  85% of sequence in fragments shorter than   20194 bp
  90% of sequence in fragments shorter than   25094 bp
  95% of sequence in fragments shorter than   35393 bp

# AvaI.NruI (CYCGRG+TCGCGA) double digest, 1020 thousand fragments (1005714+14872 sites), 2920 bp average fragment length
   5% of sequence in fragments shorter than     935 bp
  10% of sequence in fragments shorter than    1554 bp
  15% of sequence in fragments shorter than    2148 bp
  20% of sequence in fragments shorter than    2753 bp
  25% of sequence in fragments shorter than    3388 bp
  30% of sequence in fragments shorter than    4064 bp
  35% of sequence in fragments shorter than    4786 bp
  40% of sequence in fragments shorter than    5561 bp
  45% of sequence in fragments shorter than    6413 bp
  50% of sequence in fragments shorter than    7356 bp
  55% of sequence in fragments shorter than    8407 bp
  60% of sequence in fragments shorter than    9608 bp
  65% of sequence in fragments shorter than   10961 bp
  70% of sequence in fragments shorter than   12565 bp
  75% of sequence in fragments shorter than   14497 bp
  80% of sequence in fragments shorter than   16889 bp
  85% of sequence in fragments shorter than   20099 bp
  90% of sequence in fragments shorter than   24994 bp
  95% of sequence in fragments shorter than   35132 bp

# AflII.SapI (CTTAAG+GCTCTTC) double digest, 1020 thousand fragments (643284+377161 sites), 2921 bp average fragment length
   5% of sequence in fragments shorter than    1020 bp
  10% of sequence in fragments shorter than    1549 bp
  15% of sequence in fragments shorter than    1972 bp
  20% of sequence in fragments shorter than    2394 bp
  25% of sequence in fragments shorter than    2790 bp
  30% of sequence in fragments shorter than    3200 bp
  35% of sequence in fragments shorter than    3620 bp
  40% of sequence in fragments shorter than    4052 bp
  45% of sequence in fragments shorter than    4500 bp
  50% of sequence in fragments shorter than    4972 bp
  55% of sequence in fragments shorter than    5485 bp
  60% of sequence in fragments shorter than    6040 bp
  65% of sequence in fragments shorter than    6662 bp
  70% of sequence in fragments shorter than    7375 bp
  75% of sequence in fragments shorter than    8198 bp
  80% of sequence in fragments shorter than    9213 bp
  85% of sequence in fragments shorter than   10549 bp
  90% of sequence in fragments shorter than   12482 bp
  95% of sequence in fragments shorter than   16449 bp

# BspHI.PmeI (TCATGA+GTTTAAAC) double digest, 1019 thousand fragments (978290+42094 sites), 2921 bp average fragment length
   5% of sequence in fragments shorter than    1028 bp
  10% of sequence in fragments shorter than    1564 bp
  15% of sequence in fragments shorter than    2014 bp
  20% of sequence in fragments shorter than    2422 bp
  25% of sequence in fragments shorter than    2838 bp
  30% of sequence in fragments shorter than    3251 bp
  35% of sequence in fragments shorter than    3676 bp
  40% of sequence in fragments shorter than    4119 bp
  45% of sequence in fragments shorter than    4576 bp
  50% of sequence in fragments shorter than    5063 bp
  55% of sequence in fragments shorter than    5587 bp
  60% of sequence in fragments shorter than    6160 bp
  65% of sequence in fragments shorter than    6798 bp
  70% of sequence in fragments shorter than    7534 bp
  75% of sequence in fragments shorter than    8392 bp
  80% of sequence in fragments shorter than    9454 bp
  85% of sequence in fragments shorter than   10849 bp
  90% of sequence in fragments shorter than   12936 bp
  95% of sequence in fragments shorter than   17277 bp

# AflII.SmaI (CTTAAG+CCCGGG) double digest, 1019 thousand fragments (643284+376940 sites), 2921 bp average fragment length
   5% of sequence in fragments shorter than    1004 bp
  10% of sequence in fragments shorter than    1540 bp
  15% of sequence in fragments shorter than    2008 bp
  20% of sequence in fragments shorter than    2438 bp
  25% of sequence in fragments shorter than    2865 bp
  30% of sequence in fragments shorter than    3297 bp
  35% of sequence in fragments shorter than    3727 bp
  40% of sequence in fragments shorter than    4173 bp
  45% of sequence in fragments shorter than    4643 bp
  50% of sequence in fragments shorter than    5139 bp
  55% of sequence in fragments shorter than    5680 bp
  60% of sequence in fragments shorter than    6279 bp
  65% of sequence in fragments shorter than    6933 bp
  70% of sequence in fragments shorter than    7683 bp
  75% of sequence in fragments shorter than    8556 bp
  80% of sequence in fragments shorter than    9635 bp
  85% of sequence in fragments shorter than   11052 bp
  90% of sequence in fragments shorter than   13153 bp
  95% of sequence in fragments shorter than   17491 bp

# AclI.EcoRI (AACGTT+GAATTC) double digest, 1019 thousand fragments (172706+847401 sites), 2922 bp average fragment length
   5% of sequence in fragments shorter than    1000 bp
  10% of sequence in fragments shorter than    1560 bp
  15% of sequence in fragments shorter than    2042 bp
  20% of sequence in fragments shorter than    2498 bp
  25% of sequence in fragments shorter than    2936 bp
  30% of sequence in fragments shorter than    3370 bp
  35% of sequence in fragments shorter than    3811 bp
  40% of sequence in fragments shorter than    4270 bp
  45% of sequence in fragments shorter than    4748 bp
  50% of sequence in fragments shorter than    5258 bp
  55% of sequence in fragments shorter than    5807 bp
  60% of sequence in fragments shorter than    6405 bp
  65% of sequence in fragments shorter than    7069 bp
  70% of sequence in fragments shorter than    7827 bp
  75% of sequence in fragments shorter than    8696 bp
  80% of sequence in fragments shorter than    9746 bp
  85% of sequence in fragments shorter than   11133 bp
  90% of sequence in fragments shorter than   13091 bp
  95% of sequence in fragments shorter than   16653 bp

# AvaI.PvuI (CYCGRG+CGATCG) double digest, 1019 thousand fragments (1005714+14186 sites), 2922 bp average fragment length
   5% of sequence in fragments shorter than     936 bp
  10% of sequence in fragments shorter than    1554 bp
  15% of sequence in fragments shorter than    2140 bp
  20% of sequence in fragments shorter than    2743 bp
  25% of sequence in fragments shorter than    3379 bp
  30% of sequence in fragments shorter than    4052 bp
  35% of sequence in fragments shorter than    4771 bp
  40% of sequence in fragments shorter than    5543 bp
  45% of sequence in fragments shorter than    6392 bp
  50% of sequence in fragments shorter than    7341 bp
  55% of sequence in fragments shorter than    8387 bp
  60% of sequence in fragments shorter than    9584 bp
  65% of sequence in fragments shorter than   10937 bp
  70% of sequence in fragments shorter than   12528 bp
  75% of sequence in fragments shorter than   14456 bp
  80% of sequence in fragments shorter than   16834 bp
  85% of sequence in fragments shorter than   20006 bp
  90% of sequence in fragments shorter than   24848 bp
  95% of sequence in fragments shorter than   34556 bp

# AvaIII.ClaI (ATGCAT+ATCGAT) double digest, 1019 thousand fragments (933357+86477 sites), 2923 bp average fragment length
   5% of sequence in fragments shorter than    1036 bp
  10% of sequence in fragments shorter than    1576 bp
  15% of sequence in fragments shorter than    2046 bp
  20% of sequence in fragments shorter than    2476 bp
  25% of sequence in fragments shorter than    2903 bp
  30% of sequence in fragments shorter than    3340 bp
  35% of sequence in fragments shorter than    3786 bp
  40% of sequence in fragments shorter than    4250 bp
  45% of sequence in fragments shorter than    4738 bp
  50% of sequence in fragments shorter than    5260 bp
  55% of sequence in fragments shorter than    5827 bp
  60% of sequence in fragments shorter than    6450 bp
  65% of sequence in fragments shorter than    7152 bp
  70% of sequence in fragments shorter than    7955 bp
  75% of sequence in fragments shorter than    8901 bp
  80% of sequence in fragments shorter than   10059 bp
  85% of sequence in fragments shorter than   11654 bp
  90% of sequence in fragments shorter than   13987 bp
  95% of sequence in fragments shorter than   18852 bp

# HindIII.PacI (AAGCTT+TTAATTAA) double digest, 1019 thousand fragments (860365+159455 sites), 2923 bp average fragment length
   5% of sequence in fragments shorter than    1035 bp
  10% of sequence in fragments shorter than    1564 bp
  15% of sequence in fragments shorter than    1988 bp
  20% of sequence in fragments shorter than    2431 bp
  25% of sequence in fragments shorter than    2854 bp
  30% of sequence in fragments shorter than    3274 bp
  35% of sequence in fragments shorter than    3709 bp
  40% of sequence in fragments shorter than    4152 bp
  45% of sequence in fragments shorter than    4620 bp
  50% of sequence in fragments shorter than    5114 bp
  55% of sequence in fragments shorter than    5655 bp
  60% of sequence in fragments shorter than    6249 bp
  65% of sequence in fragments shorter than    6912 bp
  70% of sequence in fragments shorter than    7668 bp
  75% of sequence in fragments shorter than    8562 bp
  80% of sequence in fragments shorter than    9650 bp
  85% of sequence in fragments shorter than   11080 bp
  90% of sequence in fragments shorter than   13238 bp
  95% of sequence in fragments shorter than   17515 bp

# HaeII.HpaI (RGCGCY+GTTAAC) double digest, 1019 thousand fragments (630101+389418 sites), 2924 bp average fragment length
   5% of sequence in fragments shorter than     932 bp
  10% of sequence in fragments shorter than    1559 bp
  15% of sequence in fragments shorter than    2113 bp
  20% of sequence in fragments shorter than    2653 bp
  25% of sequence in fragments shorter than    3185 bp
  30% of sequence in fragments shorter than    3710 bp
  35% of sequence in fragments shorter than    4246 bp
  40% of sequence in fragments shorter than    4804 bp
  45% of sequence in fragments shorter than    5385 bp
  50% of sequence in fragments shorter than    6001 bp
  55% of sequence in fragments shorter than    6666 bp
  60% of sequence in fragments shorter than    7388 bp
  65% of sequence in fragments shorter than    8201 bp
  70% of sequence in fragments shorter than    9118 bp
  75% of sequence in fragments shorter than   10175 bp
  80% of sequence in fragments shorter than   11465 bp
  85% of sequence in fragments shorter than   13090 bp
  90% of sequence in fragments shorter than   15379 bp
  95% of sequence in fragments shorter than   19318 bp

# AvaI.FseI (CYCGRG+GGCCGGCC) double digest, 1019 thousand fragments (1005714+13662 sites), 2924 bp average fragment length
   5% of sequence in fragments shorter than     936 bp
  10% of sequence in fragments shorter than    1557 bp
  15% of sequence in fragments shorter than    2154 bp
  20% of sequence in fragments shorter than    2763 bp
  25% of sequence in fragments shorter than    3404 bp
  30% of sequence in fragments shorter than    4087 bp
  35% of sequence in fragments shorter than    4813 bp
  40% of sequence in fragments shorter than    5594 bp
  45% of sequence in fragments shorter than    6455 bp
  50% of sequence in fragments shorter than    7411 bp
  55% of sequence in fragments shorter than    8468 bp
  60% of sequence in fragments shorter than    9680 bp
  65% of sequence in fragments shorter than   11047 bp
  70% of sequence in fragments shorter than   12662 bp
  75% of sequence in fragments shorter than   14619 bp
  80% of sequence in fragments shorter than   17036 bp
  85% of sequence in fragments shorter than   20288 bp
  90% of sequence in fragments shorter than   25232 bp
  95% of sequence in fragments shorter than   35536 bp

# EcoRV.HgaI (GATATC+GACGC) double digest, 1017 thousand fragments (446473+571889 sites), 2927 bp average fragment length
   5% of sequence in fragments shorter than    1010 bp
  10% of sequence in fragments shorter than    1598 bp
  15% of sequence in fragments shorter than    2116 bp
  20% of sequence in fragments shorter than    2593 bp
  25% of sequence in fragments shorter than    3057 bp
  30% of sequence in fragments shorter than    3528 bp
  35% of sequence in fragments shorter than    4013 bp
  40% of sequence in fragments shorter than    4512 bp
  45% of sequence in fragments shorter than    5034 bp
  50% of sequence in fragments shorter than    5589 bp
  55% of sequence in fragments shorter than    6183 bp
  60% of sequence in fragments shorter than    6826 bp
  65% of sequence in fragments shorter than    7544 bp
  70% of sequence in fragments shorter than    8363 bp
  75% of sequence in fragments shorter than    9303 bp
  80% of sequence in fragments shorter than   10443 bp
  85% of sequence in fragments shorter than   11895 bp
  90% of sequence in fragments shorter than   13975 bp
  95% of sequence in fragments shorter than   17700 bp

# HindIII.Hpy99I (AAGCTT+CGWCG) double digest, 1017 thousand fragments (860365+157917 sites), 2927 bp average fragment length
   5% of sequence in fragments shorter than    1034 bp
  10% of sequence in fragments shorter than    1577 bp
  15% of sequence in fragments shorter than    2009 bp
  20% of sequence in fragments shorter than    2460 bp
  25% of sequence in fragments shorter than    2887 bp
  30% of sequence in fragments shorter than    3315 bp
  35% of sequence in fragments shorter than    3752 bp
  40% of sequence in fragments shorter than    4199 bp
  45% of sequence in fragments shorter than    4661 bp
  50% of sequence in fragments shorter than    5157 bp
  55% of sequence in fragments shorter than    5683 bp
  60% of sequence in fragments shorter than    6264 bp
  65% of sequence in fragments shorter than    6898 bp
  70% of sequence in fragments shorter than    7622 bp
  75% of sequence in fragments shorter than    8464 bp
  80% of sequence in fragments shorter than    9462 bp
  85% of sequence in fragments shorter than   10769 bp
  90% of sequence in fragments shorter than   12616 bp
  95% of sequence in fragments shorter than   16002 bp

# EciI.EcoRV (GGCGGA+GATATC) double digest, 1017 thousand fragments (571273+446473 sites), 2929 bp average fragment length
   5% of sequence in fragments shorter than    1003 bp
  10% of sequence in fragments shorter than    1567 bp
  15% of sequence in fragments shorter than    2063 bp
  20% of sequence in fragments shorter than    2532 bp
  25% of sequence in fragments shorter than    2991 bp
  30% of sequence in fragments shorter than    3456 bp
  35% of sequence in fragments shorter than    3929 bp
  40% of sequence in fragments shorter than    4418 bp
  45% of sequence in fragments shorter than    4939 bp
  50% of sequence in fragments shorter than    5492 bp
  55% of sequence in fragments shorter than    6088 bp
  60% of sequence in fragments shorter than    6737 bp
  65% of sequence in fragments shorter than    7468 bp
  70% of sequence in fragments shorter than    8288 bp
  75% of sequence in fragments shorter than    9250 bp
  80% of sequence in fragments shorter than   10414 bp
  85% of sequence in fragments shorter than   11934 bp
  90% of sequence in fragments shorter than   14082 bp
  95% of sequence in fragments shorter than   18081 bp

# AvaI.NotI (CYCGRG+GCGGCCGC) double digest, 1015 thousand fragments (1005714+9795 sites), 2935 bp average fragment length
   5% of sequence in fragments shorter than     943 bp
  10% of sequence in fragments shorter than    1568 bp
  15% of sequence in fragments shorter than    2167 bp
  20% of sequence in fragments shorter than    2779 bp
  25% of sequence in fragments shorter than    3422 bp
  30% of sequence in fragments shorter than    4107 bp
  35% of sequence in fragments shorter than    4837 bp
  40% of sequence in fragments shorter than    5619 bp
  45% of sequence in fragments shorter than    6483 bp
  50% of sequence in fragments shorter than    7441 bp
  55% of sequence in fragments shorter than    8502 bp
  60% of sequence in fragments shorter than    9719 bp
  65% of sequence in fragments shorter than   11091 bp
  70% of sequence in fragments shorter than   12708 bp
  75% of sequence in fragments shorter than   14671 bp
  80% of sequence in fragments shorter than   17097 bp
  85% of sequence in fragments shorter than   20341 bp
  90% of sequence in fragments shorter than   25302 bp
  95% of sequence in fragments shorter than   35663 bp

# AvaI.RsrII (CYCGRG+CGGWCCG) double digest, 1015 thousand fragments (1005714+9764 sites), 2935 bp average fragment length
   5% of sequence in fragments shorter than     940 bp
  10% of sequence in fragments shorter than    1563 bp
  15% of sequence in fragments shorter than    2160 bp
  20% of sequence in fragments shorter than    2769 bp
  25% of sequence in fragments shorter than    3411 bp
  30% of sequence in fragments shorter than    4093 bp
  35% of sequence in fragments shorter than    4820 bp
  40% of sequence in fragments shorter than    5602 bp
  45% of sequence in fragments shorter than    6459 bp
  50% of sequence in fragments shorter than    7416 bp
  55% of sequence in fragments shorter than    8474 bp
  60% of sequence in fragments shorter than    9684 bp
  65% of sequence in fragments shorter than   11052 bp
  70% of sequence in fragments shorter than   12666 bp
  75% of sequence in fragments shorter than   14622 bp
  80% of sequence in fragments shorter than   17036 bp
  85% of sequence in fragments shorter than   20273 bp
  90% of sequence in fragments shorter than   25212 bp
  95% of sequence in fragments shorter than   35495 bp

# ClaI.StuI (ATCGAT+AGGCCT) double digest, 1011 thousand fragments (86477+925729 sites), 2945 bp average fragment length
   5% of sequence in fragments shorter than     888 bp
  10% of sequence in fragments shorter than    1501 bp
  15% of sequence in fragments shorter than    2050 bp
  20% of sequence in fragments shorter than    2606 bp
  25% of sequence in fragments shorter than    3144 bp
  30% of sequence in fragments shorter than    3682 bp
  35% of sequence in fragments shorter than    4231 bp
  40% of sequence in fragments shorter than    4802 bp
  45% of sequence in fragments shorter than    5399 bp
  50% of sequence in fragments shorter than    6031 bp
  55% of sequence in fragments shorter than    6727 bp
  60% of sequence in fragments shorter than    7476 bp
  65% of sequence in fragments shorter than    8296 bp
  70% of sequence in fragments shorter than    9224 bp
  75% of sequence in fragments shorter than   10318 bp
  80% of sequence in fragments shorter than   11628 bp
  85% of sequence in fragments shorter than   13275 bp
  90% of sequence in fragments shorter than   15566 bp
  95% of sequence in fragments shorter than   19506 bp

# BsaAI.SexAI (YACGTR+ACCWGGT) double digest, 1011 thousand fragments (713801+297900 sites), 2946 bp average fragment length
   5% of sequence in fragments shorter than    1035 bp
  10% of sequence in fragments shorter than    1575 bp
  15% of sequence in fragments shorter than    2037 bp
  20% of sequence in fragments shorter than    2482 bp
  25% of sequence in fragments shorter than    2914 bp
  30% of sequence in fragments shorter than    3347 bp
  35% of sequence in fragments shorter than    3770 bp
  40% of sequence in fragments shorter than    4227 bp
  45% of sequence in fragments shorter than    4695 bp
  50% of sequence in fragments shorter than    5205 bp
  55% of sequence in fragments shorter than    5749 bp
  60% of sequence in fragments shorter than    6341 bp
  65% of sequence in fragments shorter than    6997 bp
  70% of sequence in fragments shorter than    7738 bp
  75% of sequence in fragments shorter than    8604 bp
  80% of sequence in fragments shorter than    9651 bp
  85% of sequence in fragments shorter than   10992 bp
  90% of sequence in fragments shorter than   12927 bp
  95% of sequence in fragments shorter than   16547 bp

# ApaI.SphI (GGGCCC+GCATGC) double digest, 1010 thousand fragments (460339+550952 sites), 2947 bp average fragment length
   5% of sequence in fragments shorter than     968 bp
  10% of sequence in fragments shorter than    1532 bp
  15% of sequence in fragments shorter than    2045 bp
  20% of sequence in fragments shorter than    2547 bp
  25% of sequence in fragments shorter than    3048 bp
  30% of sequence in fragments shorter than    3565 bp
  35% of sequence in fragments shorter than    4105 bp
  40% of sequence in fragments shorter than    4675 bp
  45% of sequence in fragments shorter than    5283 bp
  50% of sequence in fragments shorter than    5933 bp
  55% of sequence in fragments shorter than    6637 bp
  60% of sequence in fragments shorter than    7413 bp
  65% of sequence in fragments shorter than    8283 bp
  70% of sequence in fragments shorter than    9282 bp
  75% of sequence in fragments shorter than   10478 bp
  80% of sequence in fragments shorter than   11962 bp
  85% of sequence in fragments shorter than   13922 bp
  90% of sequence in fragments shorter than   16811 bp
  95% of sequence in fragments shorter than   22497 bp

# EcoRV.MfeI (GATATC+CAATTG) double digest, 1010 thousand fragments (446473+564303 sites), 2949 bp average fragment length
   5% of sequence in fragments shorter than    1009 bp
  10% of sequence in fragments shorter than    1515 bp
  15% of sequence in fragments shorter than    1980 bp
  20% of sequence in fragments shorter than    2419 bp
  25% of sequence in fragments shorter than    2859 bp
  30% of sequence in fragments shorter than    3297 bp
  35% of sequence in fragments shorter than    3746 bp
  40% of sequence in fragments shorter than    4209 bp
  45% of sequence in fragments shorter than    4702 bp
  50% of sequence in fragments shorter than    5229 bp
  55% of sequence in fragments shorter than    5800 bp
  60% of sequence in fragments shorter than    6439 bp
  65% of sequence in fragments shorter than    7163 bp
  70% of sequence in fragments shorter than    7996 bp
  75% of sequence in fragments shorter than    8988 bp
  80% of sequence in fragments shorter than   10243 bp
  85% of sequence in fragments shorter than   11947 bp
  90% of sequence in fragments shorter than   14530 bp
  95% of sequence in fragments shorter than   19891 bp

# AscI.AvaI (GGCGCGCC+CYCGRG) double digest, 1010 thousand fragments (4751+1005714 sites), 2950 bp average fragment length
   5% of sequence in fragments shorter than     945 bp
  10% of sequence in fragments shorter than    1571 bp
  15% of sequence in fragments shorter than    2170 bp
  20% of sequence in fragments shorter than    2782 bp
  25% of sequence in fragments shorter than    3425 bp
  30% of sequence in fragments shorter than    4109 bp
  35% of sequence in fragments shorter than    4840 bp
  40% of sequence in fragments shorter than    5623 bp
  45% of sequence in fragments shorter than    6487 bp
  50% of sequence in fragments shorter than    7445 bp
  55% of sequence in fragments shorter than    8507 bp
  60% of sequence in fragments shorter than    9723 bp
  65% of sequence in fragments shorter than   11095 bp
  70% of sequence in fragments shorter than   12713 bp
  75% of sequence in fragments shorter than   14678 bp
  80% of sequence in fragments shorter than   17104 bp
  85% of sequence in fragments shorter than   20348 bp
  90% of sequence in fragments shorter than   25305 bp
  95% of sequence in fragments shorter than   35700 bp

# PacI.XbaI (TTAATTAA+TCTAGA) double digest, 1010 thousand fragments (159455+850998 sites), 2950 bp average fragment length
   5% of sequence in fragments shorter than    1018 bp
  10% of sequence in fragments shorter than    1530 bp
  15% of sequence in fragments shorter than    2003 bp
  20% of sequence in fragments shorter than    2438 bp
  25% of sequence in fragments shorter than    2889 bp
  30% of sequence in fragments shorter than    3328 bp
  35% of sequence in fragments shorter than    3778 bp
  40% of sequence in fragments shorter than    4245 bp
  45% of sequence in fragments shorter than    4731 bp
  50% of sequence in fragments shorter than    5246 bp
  55% of sequence in fragments shorter than    5807 bp
  60% of sequence in fragments shorter than    6418 bp
  65% of sequence in fragments shorter than    7100 bp
  70% of sequence in fragments shorter than    7873 bp
  75% of sequence in fragments shorter than    8777 bp
  80% of sequence in fragments shorter than    9891 bp
  85% of sequence in fragments shorter than   11364 bp
  90% of sequence in fragments shorter than   13473 bp
  95% of sequence in fragments shorter than   17495 bp

# BglII.BsrBI (AGATCT+CCGCTC) double digest, 1009 thousand fragments (774732+235523 sites), 2951 bp average fragment length
   5% of sequence in fragments shorter than    1032 bp
  10% of sequence in fragments shorter than    1565 bp
  15% of sequence in fragments shorter than    2021 bp
  20% of sequence in fragments shorter than    2464 bp
  25% of sequence in fragments shorter than    2897 bp
  30% of sequence in fragments shorter than    3330 bp
  35% of sequence in fragments shorter than    3763 bp
  40% of sequence in fragments shorter than    4216 bp
  45% of sequence in fragments shorter than    4686 bp
  50% of sequence in fragments shorter than    5180 bp
  55% of sequence in fragments shorter than    5708 bp
  60% of sequence in fragments shorter than    6287 bp
  65% of sequence in fragments shorter than    6927 bp
  70% of sequence in fragments shorter than    7657 bp
  75% of sequence in fragments shorter than    8498 bp
  80% of sequence in fragments shorter than    9534 bp
  85% of sequence in fragments shorter than   10860 bp
  90% of sequence in fragments shorter than   12768 bp
  95% of sequence in fragments shorter than   16425 bp

# BspHI.SalI (TCATGA+GTCGAC) double digest, 1009 thousand fragments (978290+31396 sites), 2952 bp average fragment length
   5% of sequence in fragments shorter than    1042 bp
  10% of sequence in fragments shorter than    1582 bp
  15% of sequence in fragments shorter than    2037 bp
  20% of sequence in fragments shorter than    2448 bp
  25% of sequence in fragments shorter than    2870 bp
  30% of sequence in fragments shorter than    3284 bp
  35% of sequence in fragments shorter than    3712 bp
  40% of sequence in fragments shorter than    4158 bp
  45% of sequence in fragments shorter than    4615 bp
  50% of sequence in fragments shorter than    5105 bp
  55% of sequence in fragments shorter than    5630 bp
  60% of sequence in fragments shorter than    6206 bp
  65% of sequence in fragments shorter than    6842 bp
  70% of sequence in fragments shorter than    7581 bp
  75% of sequence in fragments shorter than    8442 bp
  80% of sequence in fragments shorter than    9488 bp
  85% of sequence in fragments shorter than   10872 bp
  90% of sequence in fragments shorter than   12919 bp
  95% of sequence in fragments shorter than   17123 bp

# AflII.BamHI (CTTAAG+GGATCC) double digest, 1008 thousand fragments (643284+365999 sites), 2953 bp average fragment length
   5% of sequence in fragments shorter than    1027 bp
  10% of sequence in fragments shorter than    1566 bp
  15% of sequence in fragments shorter than    2027 bp
  20% of sequence in fragments shorter than    2446 bp
  25% of sequence in fragments shorter than    2863 bp
  30% of sequence in fragments shorter than    3279 bp
  35% of sequence in fragments shorter than    3698 bp
  40% of sequence in fragments shorter than    4130 bp
  45% of sequence in fragments shorter than    4576 bp
  50% of sequence in fragments shorter than    5044 bp
  55% of sequence in fragments shorter than    5555 bp
  60% of sequence in fragments shorter than    6108 bp
  65% of sequence in fragments shorter than    6720 bp
  70% of sequence in fragments shorter than    7421 bp
  75% of sequence in fragments shorter than    8230 bp
  80% of sequence in fragments shorter than    9221 bp
  85% of sequence in fragments shorter than   10510 bp
  90% of sequence in fragments shorter than   12387 bp
  95% of sequence in fragments shorter than   16087 bp

# Hpy99I.XbaI (CGWCG+TCTAGA) double digest, 1008 thousand fragments (157917+850998 sites), 2954 bp average fragment length
   5% of sequence in fragments shorter than    1023 bp
  10% of sequence in fragments shorter than    1549 bp
  15% of sequence in fragments shorter than    2033 bp
  20% of sequence in fragments shorter than    2477 bp
  25% of sequence in fragments shorter than    2930 bp
  30% of sequence in fragments shorter than    3360 bp
  35% of sequence in fragments shorter than    3807 bp
  40% of sequence in fragments shorter than    4270 bp
  45% of sequence in fragments shorter than    4756 bp
  50% of sequence in fragments shorter than    5260 bp
  55% of sequence in fragments shorter than    5806 bp
  60% of sequence in fragments shorter than    6392 bp
  65% of sequence in fragments shorter than    7047 bp
  70% of sequence in fragments shorter than    7773 bp
  75% of sequence in fragments shorter than    8621 bp
  80% of sequence in fragments shorter than    9648 bp
  85% of sequence in fragments shorter than   10945 bp
  90% of sequence in fragments shorter than   12773 bp
  95% of sequence in fragments shorter than   15931 bp

# HaeII.SapI (RGCGCY+GCTCTTC) double digest, 1006 thousand fragments (630101+377161 sites), 2959 bp average fragment length
   5% of sequence in fragments shorter than     902 bp
  10% of sequence in fragments shorter than    1559 bp
  15% of sequence in fragments shorter than    2157 bp
  20% of sequence in fragments shorter than    2747 bp
  25% of sequence in fragments shorter than    3328 bp
  30% of sequence in fragments shorter than    3912 bp
  35% of sequence in fragments shorter than    4510 bp
  40% of sequence in fragments shorter than    5137 bp
  45% of sequence in fragments shorter than    5798 bp
  50% of sequence in fragments shorter than    6506 bp
  55% of sequence in fragments shorter than    7272 bp
  60% of sequence in fragments shorter than    8113 bp
  65% of sequence in fragments shorter than    9052 bp
  70% of sequence in fragments shorter than   10135 bp
  75% of sequence in fragments shorter than   11405 bp
  80% of sequence in fragments shorter than   12936 bp
  85% of sequence in fragments shorter than   14893 bp
  90% of sequence in fragments shorter than   17682 bp
  95% of sequence in fragments shorter than   22509 bp

# HaeII.SmaI (RGCGCY+CCCGGG) double digest, 1006 thousand fragments (630101+376940 sites), 2960 bp average fragment length
   5% of sequence in fragments shorter than     893 bp
  10% of sequence in fragments shorter than    1624 bp
  15% of sequence in fragments shorter than    2333 bp
  20% of sequence in fragments shorter than    3081 bp
  25% of sequence in fragments shorter than    3850 bp
  30% of sequence in fragments shorter than    4667 bp
  35% of sequence in fragments shorter than    5553 bp
  40% of sequence in fragments shorter than    6508 bp
  45% of sequence in fragments shorter than    7531 bp
  50% of sequence in fragments shorter than    8660 bp
  55% of sequence in fragments shorter than    9917 bp
  60% of sequence in fragments shorter than   11317 bp
  65% of sequence in fragments shorter than   12961 bp
  70% of sequence in fragments shorter than   14859 bp
  75% of sequence in fragments shorter than   17121 bp
  80% of sequence in fragments shorter than   19909 bp
  85% of sequence in fragments shorter than   23482 bp
  90% of sequence in fragments shorter than   28675 bp
  95% of sequence in fragments shorter than   37624 bp

# EcoRI.PacI (GAATTC+TTAATTAA) double digest, 1006 thousand fragments (847401+159455 sites), 2961 bp average fragment length
   5% of sequence in fragments shorter than    1009 bp
  10% of sequence in fragments shorter than    1587 bp
  15% of sequence in fragments shorter than    2087 bp
  20% of sequence in fragments shorter than    2554 bp
  25% of sequence in fragments shorter than    2998 bp
  30% of sequence in fragments shorter than    3435 bp
  35% of sequence in fragments shorter than    3890 bp
  40% of sequence in fragments shorter than    4355 bp
  45% of sequence in fragments shorter than    4850 bp
  50% of sequence in fragments shorter than    5377 bp
  55% of sequence in fragments shorter than    5938 bp
  60% of sequence in fragments shorter than    6552 bp
  65% of sequence in fragments shorter than    7234 bp
  70% of sequence in fragments shorter than    8014 bp
  75% of sequence in fragments shorter than    8920 bp
  80% of sequence in fragments shorter than   10033 bp
  85% of sequence in fragments shorter than   11481 bp
  90% of sequence in fragments shorter than   13591 bp
  95% of sequence in fragments shorter than   17443 bp

# AvaI.XhoI (CYCGRG+CTCGAG) double digest, 1005 thousand fragments (1005714+121308 sites), 2964 bp average fragment length
   5% of sequence in fragments shorter than     948 bp
  10% of sequence in fragments shorter than    1574 bp
  15% of sequence in fragments shorter than    2174 bp
  20% of sequence in fragments shorter than    2785 bp
  25% of sequence in fragments shorter than    3429 bp
  30% of sequence in fragments shorter than    4114 bp
  35% of sequence in fragments shorter than    4845 bp
  40% of sequence in fragments shorter than    5628 bp
  45% of sequence in fragments shorter than    6492 bp
  50% of sequence in fragments shorter than    7450 bp
  55% of sequence in fragments shorter than    8513 bp
  60% of sequence in fragments shorter than    9729 bp
  65% of sequence in fragments shorter than   11102 bp
  70% of sequence in fragments shorter than   12719 bp
  75% of sequence in fragments shorter than   14684 bp
  80% of sequence in fragments shorter than   17113 bp
  85% of sequence in fragments shorter than   20359 bp
  90% of sequence in fragments shorter than   25331 bp
  95% of sequence in fragments shorter than   35719 bp

# AvaI.SmaI (CYCGRG+CCCGGG) double digest, 1005 thousand fragments (1005714+376940 sites), 2964 bp average fragment length
   5% of sequence in fragments shorter than     948 bp
  10% of sequence in fragments shorter than    1574 bp
  15% of sequence in fragments shorter than    2174 bp
  20% of sequence in fragments shorter than    2785 bp
  25% of sequence in fragments shorter than    3429 bp
  30% of sequence in fragments shorter than    4114 bp
  35% of sequence in fragments shorter than    4845 bp
  40% of sequence in fragments shorter than    5628 bp
  45% of sequence in fragments shorter than    6492 bp
  50% of sequence in fragments shorter than    7450 bp
  55% of sequence in fragments shorter than    8513 bp
  60% of sequence in fragments shorter than    9729 bp
  65% of sequence in fragments shorter than   11102 bp
  70% of sequence in fragments shorter than   12719 bp
  75% of sequence in fragments shorter than   14684 bp
  80% of sequence in fragments shorter than   17113 bp
  85% of sequence in fragments shorter than   20359 bp
  90% of sequence in fragments shorter than   25331 bp
  95% of sequence in fragments shorter than   35719 bp

# EcoRI.Hpy99I (GAATTC+CGWCG) double digest, 1004 thousand fragments (847401+157917 sites), 2965 bp average fragment length
   5% of sequence in fragments shorter than    1011 bp
  10% of sequence in fragments shorter than    1610 bp
  15% of sequence in fragments shorter than    2121 bp
  20% of sequence in fragments shorter than    2594 bp
  25% of sequence in fragments shorter than    3044 bp
  30% of sequence in fragments shorter than    3475 bp
  35% of sequence in fragments shorter than    3933 bp
  40% of sequence in fragments shorter than    4404 bp
  45% of sequence in fragments shorter than    4899 bp
  50% of sequence in fragments shorter than    5420 bp
  55% of sequence in fragments shorter than    5975 bp
  60% of sequence in fragments shorter than    6582 bp
  65% of sequence in fragments shorter than    7253 bp
  70% of sequence in fragments shorter than    8001 bp
  75% of sequence in fragments shorter than    8859 bp
  80% of sequence in fragments shorter than    9882 bp
  85% of sequence in fragments shorter than   11209 bp
  90% of sequence in fragments shorter than   13043 bp
  95% of sequence in fragments shorter than   16193 bp

# BglII.KasI (AGATCT+GGCGCC) double digest, 1004 thousand fragments (774732+230381 sites), 2965 bp average fragment length
   5% of sequence in fragments shorter than    1033 bp
  10% of sequence in fragments shorter than    1563 bp
  15% of sequence in fragments shorter than    2014 bp
  20% of sequence in fragments shorter than    2453 bp
  25% of sequence in fragments shorter than    2880 bp
  30% of sequence in fragments shorter than    3312 bp
  35% of sequence in fragments shorter than    3742 bp
  40% of sequence in fragments shorter than    4191 bp
  45% of sequence in fragments shorter than    4647 bp
  50% of sequence in fragments shorter than    5130 bp
  55% of sequence in fragments shorter than    5650 bp
  60% of sequence in fragments shorter than    6221 bp
  65% of sequence in fragments shorter than    6848 bp
  70% of sequence in fragments shorter than    7576 bp
  75% of sequence in fragments shorter than    8406 bp
  80% of sequence in fragments shorter than    9443 bp
  85% of sequence in fragments shorter than   10782 bp
  90% of sequence in fragments shorter than   12745 bp
  95% of sequence in fragments shorter than   16756 bp

# AatII.AvaIII (GACGTC+ATGCAT) double digest, 1004 thousand fragments (71089+933357 sites), 2968 bp average fragment length
   5% of sequence in fragments shorter than    1054 bp
  10% of sequence in fragments shorter than    1602 bp
  15% of sequence in fragments shorter than    2079 bp
  20% of sequence in fragments shorter than    2515 bp
  25% of sequence in fragments shorter than    2950 bp
  30% of sequence in fragments shorter than    3391 bp
  35% of sequence in fragments shorter than    3842 bp
  40% of sequence in fragments shorter than    4311 bp
  45% of sequence in fragments shorter than    4807 bp
  50% of sequence in fragments shorter than    5330 bp
  55% of sequence in fragments shorter than    5902 bp
  60% of sequence in fragments shorter than    6522 bp
  65% of sequence in fragments shorter than    7220 bp
  70% of sequence in fragments shorter than    8018 bp
  75% of sequence in fragments shorter than    8954 bp
  80% of sequence in fragments shorter than   10099 bp
  85% of sequence in fragments shorter than   11598 bp
  90% of sequence in fragments shorter than   13765 bp
  95% of sequence in fragments shorter than   17990 bp

# ApaI.ScaI (GGGCCC+AGTACT) double digest, 1003 thousand fragments (460339+543793 sites), 2968 bp average fragment length
   5% of sequence in fragments shorter than    1012 bp
  10% of sequence in fragments shorter than    1571 bp
  15% of sequence in fragments shorter than    2063 bp
  20% of sequence in fragments shorter than    2533 bp
  25% of sequence in fragments shorter than    2995 bp
  30% of sequence in fragments shorter than    3465 bp
  35% of sequence in fragments shorter than    3947 bp
  40% of sequence in fragments shorter than    4447 bp
  45% of sequence in fragments shorter than    4977 bp
  50% of sequence in fragments shorter than    5542 bp
  55% of sequence in fragments shorter than    6153 bp
  60% of sequence in fragments shorter than    6822 bp
  65% of sequence in fragments shorter than    7564 bp
  70% of sequence in fragments shorter than    8404 bp
  75% of sequence in fragments shorter than    9375 bp
  80% of sequence in fragments shorter than   10577 bp
  85% of sequence in fragments shorter than   12168 bp
  90% of sequence in fragments shorter than   14481 bp
  95% of sequence in fragments shorter than   19062 bp

# SacI.SpeI (GAGCTC+ACTAGT) double digest, 1003 thousand fragments (603323+400286 sites), 2970 bp average fragment length
   5% of sequence in fragments shorter than    1051 bp
  10% of sequence in fragments shorter than    1578 bp
  15% of sequence in fragments shorter than    2037 bp
  20% of sequence in fragments shorter than    2455 bp
  25% of sequence in fragments shorter than    2872 bp
  30% of sequence in fragments shorter than    3293 bp
  35% of sequence in fragments shorter than    3675 bp
  40% of sequence in fragments shorter than    4111 bp
  45% of sequence in fragments shorter than    4565 bp
  50% of sequence in fragments shorter than    5046 bp
  55% of sequence in fragments shorter than    5565 bp
  60% of sequence in fragments shorter than    6126 bp
  65% of sequence in fragments shorter than    6745 bp
  70% of sequence in fragments shorter than    7452 bp
  75% of sequence in fragments shorter than    8264 bp
  80% of sequence in fragments shorter than    9260 bp
  85% of sequence in fragments shorter than   10548 bp
  90% of sequence in fragments shorter than   12392 bp
  95% of sequence in fragments shorter than   15818 bp

# BceAI.SexAI (ACGGC+ACCWGGT) double digest, 1002 thousand fragments (705178+297900 sites), 2972 bp average fragment length
   5% of sequence in fragments shorter than     980 bp
  10% of sequence in fragments shorter than    1568 bp
  15% of sequence in fragments shorter than    2103 bp
  20% of sequence in fragments shorter than    2626 bp
  25% of sequence in fragments shorter than    3150 bp
  30% of sequence in fragments shorter than    3686 bp
  35% of sequence in fragments shorter than    4242 bp
  40% of sequence in fragments shorter than    4817 bp
  45% of sequence in fragments shorter than    5439 bp
  50% of sequence in fragments shorter than    6091 bp
  55% of sequence in fragments shorter than    6797 bp
  60% of sequence in fragments shorter than    7577 bp
  65% of sequence in fragments shorter than    8461 bp
  70% of sequence in fragments shorter than    9456 bp
  75% of sequence in fragments shorter than   10651 bp
  80% of sequence in fragments shorter than   12126 bp
  85% of sequence in fragments shorter than   14024 bp
  90% of sequence in fragments shorter than   16750 bp
  95% of sequence in fragments shorter than   21962 bp

# BsaAI.KpnI (YACGTR+GGTACC) double digest, 1001 thousand fragments (713801+287974 sites), 2976 bp average fragment length
   5% of sequence in fragments shorter than    1035 bp
  10% of sequence in fragments shorter than    1539 bp
  15% of sequence in fragments shorter than    2011 bp
  20% of sequence in fragments shorter than    2463 bp
  25% of sequence in fragments shorter than    2905 bp
  30% of sequence in fragments shorter than    3353 bp
  35% of sequence in fragments shorter than    3805 bp
  40% of sequence in fragments shorter than    4279 bp
  45% of sequence in fragments shorter than    4773 bp
  50% of sequence in fragments shorter than    5297 bp
  55% of sequence in fragments shorter than    5859 bp
  60% of sequence in fragments shorter than    6467 bp
  65% of sequence in fragments shorter than    7132 bp
  70% of sequence in fragments shorter than    7897 bp
  75% of sequence in fragments shorter than    8787 bp
  80% of sequence in fragments shorter than    9862 bp
  85% of sequence in fragments shorter than   11219 bp
  90% of sequence in fragments shorter than   13162 bp
  95% of sequence in fragments shorter than   16746 bp

# BglII.SwaI (AGATCT+ATTTAAAT) double digest, 1000 thousand fragments (774732+226602 sites), 2977 bp average fragment length
   5% of sequence in fragments shorter than    1033 bp
  10% of sequence in fragments shorter than    1554 bp
  15% of sequence in fragments shorter than    2002 bp
  20% of sequence in fragments shorter than    2435 bp
  25% of sequence in fragments shorter than    2859 bp
  30% of sequence in fragments shorter than    3290 bp
  35% of sequence in fragments shorter than    3722 bp
  40% of sequence in fragments shorter than    4173 bp
  45% of sequence in fragments shorter than    4638 bp
  50% of sequence in fragments shorter than    5131 bp
  55% of sequence in fragments shorter than    5668 bp
  60% of sequence in fragments shorter than    6257 bp
  65% of sequence in fragments shorter than    6916 bp
  70% of sequence in fragments shorter than    7684 bp
  75% of sequence in fragments shorter than    8577 bp
  80% of sequence in fragments shorter than    9680 bp
  85% of sequence in fragments shorter than   11140 bp
  90% of sequence in fragments shorter than   13421 bp
  95% of sequence in fragments shorter than   18252 bp

# BsaAI.NheI (YACGTR+GCTAGC) double digest, 1000 thousand fragments (713801+287384 sites), 2977 bp average fragment length
   5% of sequence in fragments shorter than    1032 bp
  10% of sequence in fragments shorter than    1584 bp
  15% of sequence in fragments shorter than    2009 bp
  20% of sequence in fragments shorter than    2468 bp
  25% of sequence in fragments shorter than    2909 bp
  30% of sequence in fragments shorter than    3352 bp
  35% of sequence in fragments shorter than    3809 bp
  40% of sequence in fragments shorter than    4281 bp
  45% of sequence in fragments shorter than    4779 bp
  50% of sequence in fragments shorter than    5302 bp
  55% of sequence in fragments shorter than    5866 bp
  60% of sequence in fragments shorter than    6473 bp
  65% of sequence in fragments shorter than    7146 bp
  70% of sequence in fragments shorter than    7906 bp
  75% of sequence in fragments shorter than    8780 bp
  80% of sequence in fragments shorter than    9859 bp
  85% of sequence in fragments shorter than   11229 bp
  90% of sequence in fragments shorter than   13172 bp
  95% of sequence in fragments shorter than   16665 bp

# AvaIII.SacII (ATGCAT+CCGCGG) double digest, 999 thousand fragments (933357+66901 sites), 2980 bp average fragment length
   5% of sequence in fragments shorter than    1063 bp
  10% of sequence in fragments shorter than    1627 bp
  15% of sequence in fragments shorter than    2107 bp
  20% of sequence in fragments shorter than    2568 bp
  25% of sequence in fragments shorter than    3014 bp
  30% of sequence in fragments shorter than    3464 bp
  35% of sequence in fragments shorter than    3929 bp
  40% of sequence in fragments shorter than    4412 bp
  45% of sequence in fragments shorter than    4913 bp
  50% of sequence in fragments shorter than    5450 bp
  55% of sequence in fragments shorter than    6032 bp
  60% of sequence in fragments shorter than    6668 bp
  65% of sequence in fragments shorter than    7380 bp
  70% of sequence in fragments shorter than    8183 bp
  75% of sequence in fragments shorter than    9120 bp
  80% of sequence in fragments shorter than   10280 bp
  85% of sequence in fragments shorter than   11803 bp
  90% of sequence in fragments shorter than   13976 bp
  95% of sequence in fragments shorter than   18274 bp

# BspHI.MluI (TCATGA+ACGCGT) double digest, 999 thousand fragments (978290+21415 sites), 2981 bp average fragment length
   5% of sequence in fragments shorter than    1053 bp
  10% of sequence in fragments shorter than    1601 bp
  15% of sequence in fragments shorter than    2059 bp
  20% of sequence in fragments shorter than    2475 bp
  25% of sequence in fragments shorter than    2901 bp
  30% of sequence in fragments shorter than    3319 bp
  35% of sequence in fragments shorter than    3754 bp
  40% of sequence in fragments shorter than    4200 bp
  45% of sequence in fragments shorter than    4664 bp
  50% of sequence in fragments shorter than    5159 bp
  55% of sequence in fragments shorter than    5687 bp
  60% of sequence in fragments shorter than    6267 bp
  65% of sequence in fragments shorter than    6905 bp
  70% of sequence in fragments shorter than    7646 bp
  75% of sequence in fragments shorter than    8512 bp
  80% of sequence in fragments shorter than    9556 bp
  85% of sequence in fragments shorter than   10945 bp
  90% of sequence in fragments shorter than   12988 bp
  95% of sequence in fragments shorter than   17110 bp

# EcoRV.SphI (GATATC+GCATGC) double digest, 996 thousand fragments (446473+550952 sites), 2989 bp average fragment length
   5% of sequence in fragments shorter than    1047 bp
  10% of sequence in fragments shorter than    1589 bp
  15% of sequence in fragments shorter than    2045 bp
  20% of sequence in fragments shorter than    2476 bp
  25% of sequence in fragments shorter than    2895 bp
  30% of sequence in fragments shorter than    3318 bp
  35% of sequence in fragments shorter than    3741 bp
  40% of sequence in fragments shorter than    4175 bp
  45% of sequence in fragments shorter than    4628 bp
  50% of sequence in fragments shorter than    5106 bp
  55% of sequence in fragments shorter than    5617 bp
  60% of sequence in fragments shorter than    6179 bp
  65% of sequence in fragments shorter than    6796 bp
  70% of sequence in fragments shorter than    7490 bp
  75% of sequence in fragments shorter than    8288 bp
  80% of sequence in fragments shorter than    9257 bp
  85% of sequence in fragments shorter than   10514 bp
  90% of sequence in fragments shorter than   12309 bp
  95% of sequence in fragments shorter than   15558 bp

# AatII.StuI (GACGTC+AGGCCT) double digest, 996 thousand fragments (71089+925729 sites), 2990 bp average fragment length
   5% of sequence in fragments shorter than     893 bp
  10% of sequence in fragments shorter than    1514 bp
  15% of sequence in fragments shorter than    2078 bp
  20% of sequence in fragments shorter than    2655 bp
  25% of sequence in fragments shorter than    3217 bp
  30% of sequence in fragments shorter than    3775 bp
  35% of sequence in fragments shorter than    4358 bp
  40% of sequence in fragments shorter than    4961 bp
  45% of sequence in fragments shorter than    5592 bp
  50% of sequence in fragments shorter than    6267 bp
  55% of sequence in fragments shorter than    7003 bp
  60% of sequence in fragments shorter than    7801 bp
  65% of sequence in fragments shorter than    8671 bp
  70% of sequence in fragments shorter than    9682 bp
  75% of sequence in fragments shorter than   10852 bp
  80% of sequence in fragments shorter than   12242 bp
  85% of sequence in fragments shorter than   14028 bp
  90% of sequence in fragments shorter than   16499 bp
  95% of sequence in fragments shorter than   20771 bp

# BamHI.HaeII (GGATCC+RGCGCY) double digest, 995 thousand fragments (365999+630101 sites), 2993 bp average fragment length
   5% of sequence in fragments shorter than     924 bp
  10% of sequence in fragments shorter than    1589 bp
  15% of sequence in fragments shorter than    2193 bp
  20% of sequence in fragments shorter than    2791 bp
  25% of sequence in fragments shorter than    3381 bp
  30% of sequence in fragments shorter than    3966 bp
  35% of sequence in fragments shorter than    4585 bp
  40% of sequence in fragments shorter than    5237 bp
  45% of sequence in fragments shorter than    5935 bp
  50% of sequence in fragments shorter than    6686 bp
  55% of sequence in fragments shorter than    7505 bp
  60% of sequence in fragments shorter than    8398 bp
  65% of sequence in fragments shorter than    9420 bp
  70% of sequence in fragments shorter than   10582 bp
  75% of sequence in fragments shorter than   11916 bp
  80% of sequence in fragments shorter than   13552 bp
  85% of sequence in fragments shorter than   15675 bp
  90% of sequence in fragments shorter than   18706 bp
  95% of sequence in fragments shorter than   23948 bp

# AvrII.SpeI (CCTAGG+ACTAGT) double digest, 994 thousand fragments (594956+400286 sites), 2995 bp average fragment length
   5% of sequence in fragments shorter than    1028 bp
  10% of sequence in fragments shorter than    1579 bp
  15% of sequence in fragments shorter than    2055 bp
  20% of sequence in fragments shorter than    2489 bp
  25% of sequence in fragments shorter than    2921 bp
  30% of sequence in fragments shorter than    3350 bp
  35% of sequence in fragments shorter than    3785 bp
  40% of sequence in fragments shorter than    4231 bp
  45% of sequence in fragments shorter than    4692 bp
  50% of sequence in fragments shorter than    5177 bp
  55% of sequence in fragments shorter than    5691 bp
  60% of sequence in fragments shorter than    6254 bp
  65% of sequence in fragments shorter than    6883 bp
  70% of sequence in fragments shorter than    7602 bp
  75% of sequence in fragments shorter than    8432 bp
  80% of sequence in fragments shorter than    9446 bp
  85% of sequence in fragments shorter than   10758 bp
  90% of sequence in fragments shorter than   12636 bp
  95% of sequence in fragments shorter than   16341 bp

# BsrBI.NcoI (CCGCTC+CCATGG) double digest, 994 thousand fragments (235523+759594 sites), 2995 bp average fragment length
   5% of sequence in fragments shorter than    1022 bp
  10% of sequence in fragments shorter than    1589 bp
  15% of sequence in fragments shorter than    2101 bp
  20% of sequence in fragments shorter than    2596 bp
  25% of sequence in fragments shorter than    3064 bp
  30% of sequence in fragments shorter than    3554 bp
  35% of sequence in fragments shorter than    4061 bp
  40% of sequence in fragments shorter than    4571 bp
  45% of sequence in fragments shorter than    5122 bp
  50% of sequence in fragments shorter than    5699 bp
  55% of sequence in fragments shorter than    6321 bp
  60% of sequence in fragments shorter than    7020 bp
  65% of sequence in fragments shorter than    7799 bp
  70% of sequence in fragments shorter than    8696 bp
  75% of sequence in fragments shorter than    9750 bp
  80% of sequence in fragments shorter than   11024 bp
  85% of sequence in fragments shorter than   12705 bp
  90% of sequence in fragments shorter than   15105 bp
  95% of sequence in fragments shorter than   19814 bp

# BciVI.SexAI (GTATCC+ACCWGGT) double digest, 993 thousand fragments (696093+297900 sites), 2999 bp average fragment length
   5% of sequence in fragments shorter than    1067 bp
  10% of sequence in fragments shorter than    1609 bp
  15% of sequence in fragments shorter than    2054 bp
  20% of sequence in fragments shorter than    2475 bp
  25% of sequence in fragments shorter than    2901 bp
  30% of sequence in fragments shorter than    3321 bp
  35% of sequence in fragments shorter than    3748 bp
  40% of sequence in fragments shorter than    4182 bp
  45% of sequence in fragments shorter than    4636 bp
  50% of sequence in fragments shorter than    5121 bp
  55% of sequence in fragments shorter than    5645 bp
  60% of sequence in fragments shorter than    6211 bp
  65% of sequence in fragments shorter than    6836 bp
  70% of sequence in fragments shorter than    7545 bp
  75% of sequence in fragments shorter than    8371 bp
  80% of sequence in fragments shorter than    9382 bp
  85% of sequence in fragments shorter than   10679 bp
  90% of sequence in fragments shorter than   12524 bp
  95% of sequence in fragments shorter than   15996 bp

# BspHI.SgrAI (TCATGA+CRCCGGYG) double digest, 993 thousand fragments (978290+15270 sites), 3000 bp average fragment length
   5% of sequence in fragments shorter than    1060 bp
  10% of sequence in fragments shorter than    1611 bp
  15% of sequence in fragments shorter than    2070 bp
  20% of sequence in fragments shorter than    2490 bp
  25% of sequence in fragments shorter than    2919 bp
  30% of sequence in fragments shorter than    3339 bp
  35% of sequence in fragments shorter than    3776 bp
  40% of sequence in fragments shorter than    4225 bp
  45% of sequence in fragments shorter than    4691 bp
  50% of sequence in fragments shorter than    5188 bp
  55% of sequence in fragments shorter than    5717 bp
  60% of sequence in fragments shorter than    6300 bp
  65% of sequence in fragments shorter than    6945 bp
  70% of sequence in fragments shorter than    7688 bp
  75% of sequence in fragments shorter than    8554 bp
  80% of sequence in fragments shorter than    9613 bp
  85% of sequence in fragments shorter than   11010 bp
  90% of sequence in fragments shorter than   13069 bp
  95% of sequence in fragments shorter than   17211 bp

# BspHI.NruI (TCATGA+TCGCGA) double digest, 992 thousand fragments (978290+14872 sites), 3001 bp average fragment length
   5% of sequence in fragments shorter than    1059 bp
  10% of sequence in fragments shorter than    1611 bp
  15% of sequence in fragments shorter than    2070 bp
  20% of sequence in fragments shorter than    2490 bp
  25% of sequence in fragments shorter than    2918 bp
  30% of sequence in fragments shorter than    3340 bp
  35% of sequence in fragments shorter than    3777 bp
  40% of sequence in fragments shorter than    4227 bp
  45% of sequence in fragments shorter than    4695 bp
  50% of sequence in fragments shorter than    5193 bp
  55% of sequence in fragments shorter than    5725 bp
  60% of sequence in fragments shorter than    6312 bp
  65% of sequence in fragments shorter than    6956 bp
  70% of sequence in fragments shorter than    7702 bp
  75% of sequence in fragments shorter than    8571 bp
  80% of sequence in fragments shorter than    9639 bp
  85% of sequence in fragments shorter than   11051 bp
  90% of sequence in fragments shorter than   13147 bp
  95% of sequence in fragments shorter than   17393 bp

# BceAI.KpnI (ACGGC+GGTACC) double digest, 992 thousand fragments (705178+287974 sites), 3001 bp average fragment length
   5% of sequence in fragments shorter than    1018 bp
  10% of sequence in fragments shorter than    1576 bp
  15% of sequence in fragments shorter than    2094 bp
  20% of sequence in fragments shorter than    2625 bp
  25% of sequence in fragments shorter than    3134 bp
  30% of sequence in fragments shorter than    3660 bp
  35% of sequence in fragments shorter than    4203 bp
  40% of sequence in fragments shorter than    4781 bp
  45% of sequence in fragments shorter than    5389 bp
  50% of sequence in fragments shorter than    6040 bp
  55% of sequence in fragments shorter than    6737 bp
  60% of sequence in fragments shorter than    7515 bp
  65% of sequence in fragments shorter than    8381 bp
  70% of sequence in fragments shorter than    9361 bp
  75% of sequence in fragments shorter than   10525 bp
  80% of sequence in fragments shorter than   11965 bp
  85% of sequence in fragments shorter than   13795 bp
  90% of sequence in fragments shorter than   16452 bp
  95% of sequence in fragments shorter than   21403 bp

# HpaI.SacI (GTTAAC+GAGCTC) double digest, 992 thousand fragments (389418+603323 sites), 3002 bp average fragment length
   5% of sequence in fragments shorter than    1046 bp
  10% of sequence in fragments shorter than    1585 bp
  15% of sequence in fragments shorter than    2055 bp
  20% of sequence in fragments shorter than    2488 bp
  25% of sequence in fragments shorter than    2921 bp
  30% of sequence in fragments shorter than    3354 bp
  35% of sequence in fragments shorter than    3796 bp
  40% of sequence in fragments shorter than    4242 bp
  45% of sequence in fragments shorter than    4701 bp
  50% of sequence in fragments shorter than    5195 bp
  55% of sequence in fragments shorter than    5722 bp
  60% of sequence in fragments shorter than    6303 bp
  65% of sequence in fragments shorter than    6949 bp
  70% of sequence in fragments shorter than    7683 bp
  75% of sequence in fragments shorter than    8519 bp
  80% of sequence in fragments shorter than    9562 bp
  85% of sequence in fragments shorter than   10900 bp
  90% of sequence in fragments shorter than   12825 bp
  95% of sequence in fragments shorter than   16527 bp

# HindIII.SnaBI (AAGCTT+TACGTA) double digest, 992 thousand fragments (860365+132299 sites), 3003 bp average fragment length
   5% of sequence in fragments shorter than    1060 bp
  10% of sequence in fragments shorter than    1593 bp
  15% of sequence in fragments shorter than    2027 bp
  20% of sequence in fragments shorter than    2474 bp
  25% of sequence in fragments shorter than    2902 bp
  30% of sequence in fragments shorter than    3330 bp
  35% of sequence in fragments shorter than    3774 bp
  40% of sequence in fragments shorter than    4227 bp
  45% of sequence in fragments shorter than    4703 bp
  50% of sequence in fragments shorter than    5209 bp
  55% of sequence in fragments shorter than    5753 bp
  60% of sequence in fragments shorter than    6352 bp
  65% of sequence in fragments shorter than    7017 bp
  70% of sequence in fragments shorter than    7779 bp
  75% of sequence in fragments shorter than    8667 bp
  80% of sequence in fragments shorter than    9764 bp
  85% of sequence in fragments shorter than   11202 bp
  90% of sequence in fragments shorter than   13308 bp
  95% of sequence in fragments shorter than   17359 bp

# SacII.StuI (CCGCGG+AGGCCT) double digest, 992 thousand fragments (66901+925729 sites), 3003 bp average fragment length
   5% of sequence in fragments shorter than     886 bp
  10% of sequence in fragments shorter than    1537 bp
  15% of sequence in fragments shorter than    2129 bp
  20% of sequence in fragments shorter than    2740 bp
  25% of sequence in fragments shorter than    3330 bp
  30% of sequence in fragments shorter than    3924 bp
  35% of sequence in fragments shorter than    4533 bp
  40% of sequence in fragments shorter than    5172 bp
  45% of sequence in fragments shorter than    5842 bp
  50% of sequence in fragments shorter than    6554 bp
  55% of sequence in fragments shorter than    7330 bp
  60% of sequence in fragments shorter than    8161 bp
  65% of sequence in fragments shorter than    9088 bp
  70% of sequence in fragments shorter than   10147 bp
  75% of sequence in fragments shorter than   11382 bp
  80% of sequence in fragments shorter than   12834 bp
  85% of sequence in fragments shorter than   14735 bp
  90% of sequence in fragments shorter than   17340 bp
  95% of sequence in fragments shorter than   21862 bp

# AvaIII.PspXI (ATGCAT+VCTCGAGB) double digest, 992 thousand fragments (933357+59193 sites), 3003 bp average fragment length
   5% of sequence in fragments shorter than    1067 bp
  10% of sequence in fragments shorter than    1621 bp
  15% of sequence in fragments shorter than    2091 bp
  20% of sequence in fragments shorter than    2543 bp
  25% of sequence in fragments shorter than    2981 bp
  30% of sequence in fragments shorter than    3427 bp
  35% of sequence in fragments shorter than    3883 bp
  40% of sequence in fragments shorter than    4359 bp
  45% of sequence in fragments shorter than    4857 bp
  50% of sequence in fragments shorter than    5387 bp
  55% of sequence in fragments shorter than    5963 bp
  60% of sequence in fragments shorter than    6593 bp
  65% of sequence in fragments shorter than    7300 bp
  70% of sequence in fragments shorter than    8091 bp
  75% of sequence in fragments shorter than    9025 bp
  80% of sequence in fragments shorter than   10180 bp
  85% of sequence in fragments shorter than   11712 bp
  90% of sequence in fragments shorter than   13920 bp
  95% of sequence in fragments shorter than   18298 bp

# BceAI.NheI (ACGGC+GCTAGC) double digest, 992 thousand fragments (705178+287384 sites), 3003 bp average fragment length
   5% of sequence in fragments shorter than    1032 bp
  10% of sequence in fragments shorter than    1641 bp
  15% of sequence in fragments shorter than    2113 bp
  20% of sequence in fragments shorter than    2624 bp
  25% of sequence in fragments shorter than    3119 bp
  30% of sequence in fragments shorter than    3650 bp
  35% of sequence in fragments shorter than    4192 bp
  40% of sequence in fragments shorter than    4765 bp
  45% of sequence in fragments shorter than    5367 bp
  50% of sequence in fragments shorter than    6001 bp
  55% of sequence in fragments shorter than    6685 bp
  60% of sequence in fragments shorter than    7444 bp
  65% of sequence in fragments shorter than    8290 bp
  70% of sequence in fragments shorter than    9245 bp
  75% of sequence in fragments shorter than   10387 bp
  80% of sequence in fragments shorter than   11771 bp
  85% of sequence in fragments shorter than   13559 bp
  90% of sequence in fragments shorter than   16110 bp
  95% of sequence in fragments shorter than   20654 bp

# BspHI.PvuI (TCATGA+CGATCG) double digest, 992 thousand fragments (978290+14186 sites), 3003 bp average fragment length
   5% of sequence in fragments shorter than    1060 bp
  10% of sequence in fragments shorter than    1613 bp
  15% of sequence in fragments shorter than    2068 bp
  20% of sequence in fragments shorter than    2489 bp
  25% of sequence in fragments shorter than    2919 bp
  30% of sequence in fragments shorter than    3341 bp
  35% of sequence in fragments shorter than    3779 bp
  40% of sequence in fragments shorter than    4230 bp
  45% of sequence in fragments shorter than    4699 bp
  50% of sequence in fragments shorter than    5198 bp
  55% of sequence in fragments shorter than    5733 bp
  60% of sequence in fragments shorter than    6322 bp
  65% of sequence in fragments shorter than    6970 bp
  70% of sequence in fragments shorter than    7720 bp
  75% of sequence in fragments shorter than    8591 bp
  80% of sequence in fragments shorter than    9660 bp
  85% of sequence in fragments shorter than   11071 bp
  90% of sequence in fragments shorter than   13153 bp
  95% of sequence in fragments shorter than   17374 bp

# BspHI.FseI (TCATGA+GGCCGGCC) double digest, 991 thousand fragments (978290+13662 sites), 3005 bp average fragment length
   5% of sequence in fragments shorter than    1062 bp
  10% of sequence in fragments shorter than    1615 bp
  15% of sequence in fragments shorter than    2074 bp
  20% of sequence in fragments shorter than    2494 bp
  25% of sequence in fragments shorter than    2923 bp
  30% of sequence in fragments shorter than    3344 bp
  35% of sequence in fragments shorter than    3782 bp
  40% of sequence in fragments shorter than    4230 bp
  45% of sequence in fragments shorter than    4699 bp
  50% of sequence in fragments shorter than    5196 bp
  55% of sequence in fragments shorter than    5727 bp
  60% of sequence in fragments shorter than    6311 bp
  65% of sequence in fragments shorter than    6954 bp
  70% of sequence in fragments shorter than    7697 bp
  75% of sequence in fragments shorter than    8562 bp
  80% of sequence in fragments shorter than    9619 bp
  85% of sequence in fragments shorter than   11023 bp
  90% of sequence in fragments shorter than   13076 bp
  95% of sequence in fragments shorter than   17209 bp

# EcoRV.ScaI (GATATC+AGTACT) double digest, 989 thousand fragments (446473+543793 sites), 3010 bp average fragment length
   5% of sequence in fragments shorter than    1034 bp
  10% of sequence in fragments shorter than    1575 bp
  15% of sequence in fragments shorter than    2041 bp
  20% of sequence in fragments shorter than    2485 bp
  25% of sequence in fragments shorter than    2923 bp
  30% of sequence in fragments shorter than    3362 bp
  35% of sequence in fragments shorter than    3804 bp
  40% of sequence in fragments shorter than    4270 bp
  45% of sequence in fragments shorter than    4750 bp
  50% of sequence in fragments shorter than    5271 bp
  55% of sequence in fragments shorter than    5841 bp
  60% of sequence in fragments shorter than    6462 bp
  65% of sequence in fragments shorter than    7167 bp
  70% of sequence in fragments shorter than    7982 bp
  75% of sequence in fragments shorter than    8947 bp
  80% of sequence in fragments shorter than   10146 bp
  85% of sequence in fragments shorter than   11795 bp
  90% of sequence in fragments shorter than   14234 bp
  95% of sequence in fragments shorter than   19354 bp

# KasI.NcoI (GGCGCC+CCATGG) double digest, 989 thousand fragments (230381+759594 sites), 3011 bp average fragment length
   5% of sequence in fragments shorter than    1034 bp
  10% of sequence in fragments shorter than    1592 bp
  15% of sequence in fragments shorter than    2095 bp
  20% of sequence in fragments shorter than    2572 bp
  25% of sequence in fragments shorter than    3040 bp
  30% of sequence in fragments shorter than    3511 bp
  35% of sequence in fragments shorter than    3997 bp
  40% of sequence in fragments shorter than    4497 bp
  45% of sequence in fragments shorter than    5001 bp
  50% of sequence in fragments shorter than    5516 bp
  55% of sequence in fragments shorter than    6096 bp
  60% of sequence in fragments shorter than    6752 bp
  65% of sequence in fragments shorter than    7502 bp
  70% of sequence in fragments shorter than    8355 bp
  75% of sequence in fragments shorter than    9352 bp
  80% of sequence in fragments shorter than   10589 bp
  85% of sequence in fragments shorter than   12198 bp
  90% of sequence in fragments shorter than   14556 bp
  95% of sequence in fragments shorter than   19340 bp

# ClaI.NdeI (ATCGAT+CATATG) double digest, 989 thousand fragments (86477+903360 sites), 3011 bp average fragment length
   5% of sequence in fragments shorter than    1013 bp
  10% of sequence in fragments shorter than    1548 bp
  15% of sequence in fragments shorter than    2055 bp
  20% of sequence in fragments shorter than    2538 bp
  25% of sequence in fragments shorter than    3007 bp
  30% of sequence in fragments shorter than    3482 bp
  35% of sequence in fragments shorter than    3972 bp
  40% of sequence in fragments shorter than    4478 bp
  45% of sequence in fragments shorter than    5000 bp
  50% of sequence in fragments shorter than    5557 bp
  55% of sequence in fragments shorter than    6164 bp
  60% of sequence in fragments shorter than    6836 bp
  65% of sequence in fragments shorter than    7607 bp
  70% of sequence in fragments shorter than    8503 bp
  75% of sequence in fragments shorter than    9566 bp
  80% of sequence in fragments shorter than   10908 bp
  85% of sequence in fragments shorter than   12737 bp
  90% of sequence in fragments shorter than   15647 bp
  95% of sequence in fragments shorter than   22268 bp

# BspHI.NotI (TCATGA+GCGGCCGC) double digest, 987 thousand fragments (978290+9795 sites), 3017 bp average fragment length
   5% of sequence in fragments shorter than    1065 bp
  10% of sequence in fragments shorter than    1622 bp
  15% of sequence in fragments shorter than    2083 bp
  20% of sequence in fragments shorter than    2505 bp
  25% of sequence in fragments shorter than    2937 bp
  30% of sequence in fragments shorter than    3360 bp
  35% of sequence in fragments shorter than    3800 bp
  40% of sequence in fragments shorter than    4251 bp
  45% of sequence in fragments shorter than    4721 bp
  50% of sequence in fragments shorter than    5221 bp
  55% of sequence in fragments shorter than    5757 bp
  60% of sequence in fragments shorter than    6346 bp
  65% of sequence in fragments shorter than    6991 bp
  70% of sequence in fragments shorter than    7740 bp
  75% of sequence in fragments shorter than    8613 bp
  80% of sequence in fragments shorter than    9683 bp
  85% of sequence in fragments shorter than   11093 bp
  90% of sequence in fragments shorter than   13175 bp
  95% of sequence in fragments shorter than   17391 bp

# BspHI.RsrII (TCATGA+CGGWCCG) double digest, 987 thousand fragments (978290+9764 sites), 3017 bp average fragment length
   5% of sequence in fragments shorter than    1065 bp
  10% of sequence in fragments shorter than    1621 bp
  15% of sequence in fragments shorter than    2081 bp
  20% of sequence in fragments shorter than    2503 bp
  25% of sequence in fragments shorter than    2935 bp
  30% of sequence in fragments shorter than    3357 bp
  35% of sequence in fragments shorter than    3798 bp
  40% of sequence in fragments shorter than    4248 bp
  45% of sequence in fragments shorter than    4719 bp
  50% of sequence in fragments shorter than    5218 bp
  55% of sequence in fragments shorter than    5755 bp
  60% of sequence in fragments shorter than    6345 bp
  65% of sequence in fragments shorter than    6991 bp
  70% of sequence in fragments shorter than    7740 bp
  75% of sequence in fragments shorter than    8615 bp
  80% of sequence in fragments shorter than    9690 bp
  85% of sequence in fragments shorter than   11106 bp
  90% of sequence in fragments shorter than   13209 bp
  95% of sequence in fragments shorter than   17455 bp

# AgeI.AvaIII (ACCGGT+ATGCAT) double digest, 986 thousand fragments (53386+933357 sites), 3021 bp average fragment length
   5% of sequence in fragments shorter than    1072 bp
  10% of sequence in fragments shorter than    1632 bp
  15% of sequence in fragments shorter than    2107 bp
  20% of sequence in fragments shorter than    2564 bp
  25% of sequence in fragments shorter than    3006 bp
  30% of sequence in fragments shorter than    3454 bp
  35% of sequence in fragments shorter than    3913 bp
  40% of sequence in fragments shorter than    4390 bp
  45% of sequence in fragments shorter than    4886 bp
  50% of sequence in fragments shorter than    5416 bp
  55% of sequence in fragments shorter than    5995 bp
  60% of sequence in fragments shorter than    6626 bp
  65% of sequence in fragments shorter than    7337 bp
  70% of sequence in fragments shorter than    8147 bp
  75% of sequence in fragments shorter than    9100 bp
  80% of sequence in fragments shorter than   10291 bp
  85% of sequence in fragments shorter than   11861 bp
  90% of sequence in fragments shorter than   14136 bp
  95% of sequence in fragments shorter than   18806 bp

# NcoI.SwaI (CCATGG+ATTTAAAT) double digest, 985 thousand fragments (759594+226602 sites), 3022 bp average fragment length
   5% of sequence in fragments shorter than    1060 bp
  10% of sequence in fragments shorter than    1603 bp
  15% of sequence in fragments shorter than    2081 bp
  20% of sequence in fragments shorter than    2529 bp
  25% of sequence in fragments shorter than    2963 bp
  30% of sequence in fragments shorter than    3405 bp
  35% of sequence in fragments shorter than    3850 bp
  40% of sequence in fragments shorter than    4310 bp
  45% of sequence in fragments shorter than    4767 bp
  50% of sequence in fragments shorter than    5277 bp
  55% of sequence in fragments shorter than    5804 bp
  60% of sequence in fragments shorter than    6381 bp
  65% of sequence in fragments shorter than    7032 bp
  70% of sequence in fragments shorter than    7775 bp
  75% of sequence in fragments shorter than    8644 bp
  80% of sequence in fragments shorter than    9708 bp
  85% of sequence in fragments shorter than   11090 bp
  90% of sequence in fragments shorter than   13096 bp
  95% of sequence in fragments shorter than   17026 bp

# HindIII.NaeI (AAGCTT+GCCGGC) double digest, 985 thousand fragments (860365+125692 sites), 3023 bp average fragment length
   5% of sequence in fragments shorter than    1067 bp
  10% of sequence in fragments shorter than    1625 bp
  15% of sequence in fragments shorter than    2070 bp
  20% of sequence in fragments shorter than    2530 bp
  25% of sequence in fragments shorter than    2968 bp
  30% of sequence in fragments shorter than    3409 bp
  35% of sequence in fragments shorter than    3853 bp
  40% of sequence in fragments shorter than    4313 bp
  45% of sequence in fragments shorter than    4788 bp
  50% of sequence in fragments shorter than    5289 bp
  55% of sequence in fragments shorter than    5829 bp
  60% of sequence in fragments shorter than    6424 bp
  65% of sequence in fragments shorter than    7077 bp
  70% of sequence in fragments shorter than    7824 bp
  75% of sequence in fragments shorter than    8686 bp
  80% of sequence in fragments shorter than    9726 bp
  85% of sequence in fragments shorter than   11064 bp
  90% of sequence in fragments shorter than   12965 bp
  95% of sequence in fragments shorter than   16611 bp

# PspXI.StuI (VCTCGAGB+AGGCCT) double digest, 984 thousand fragments (59193+925729 sites), 3027 bp average fragment length
   5% of sequence in fragments shorter than     904 bp
  10% of sequence in fragments shorter than    1537 bp
  15% of sequence in fragments shorter than    2107 bp
  20% of sequence in fragments shorter than    2696 bp
  25% of sequence in fragments shorter than    3265 bp
  30% of sequence in fragments shorter than    3837 bp
  35% of sequence in fragments shorter than    4423 bp
  40% of sequence in fragments shorter than    5038 bp
  45% of sequence in fragments shorter than    5685 bp
  50% of sequence in fragments shorter than    6371 bp
  55% of sequence in fragments shorter than    7120 bp
  60% of sequence in fragments shorter than    7922 bp
  65% of sequence in fragments shorter than    8812 bp
  70% of sequence in fragments shorter than    9839 bp
  75% of sequence in fragments shorter than   11030 bp
  80% of sequence in fragments shorter than   12451 bp
  85% of sequence in fragments shorter than   14249 bp
  90% of sequence in fragments shorter than   16751 bp
  95% of sequence in fragments shorter than   21152 bp

# AvrII.HpaI (CCTAGG+GTTAAC) double digest, 984 thousand fragments (594956+389418 sites), 3028 bp average fragment length
   5% of sequence in fragments shorter than    1060 bp
  10% of sequence in fragments shorter than    1613 bp
  15% of sequence in fragments shorter than    2064 bp
  20% of sequence in fragments shorter than    2500 bp
  25% of sequence in fragments shorter than    2925 bp
  30% of sequence in fragments shorter than    3349 bp
  35% of sequence in fragments shorter than    3779 bp
  40% of sequence in fragments shorter than    4220 bp
  45% of sequence in fragments shorter than    4678 bp
  50% of sequence in fragments shorter than    5163 bp
  55% of sequence in fragments shorter than    5684 bp
  60% of sequence in fragments shorter than    6254 bp
  65% of sequence in fragments shorter than    6883 bp
  70% of sequence in fragments shorter than    7610 bp
  75% of sequence in fragments shorter than    8444 bp
  80% of sequence in fragments shorter than    9472 bp
  85% of sequence in fragments shorter than   10790 bp
  90% of sequence in fragments shorter than   12740 bp
  95% of sequence in fragments shorter than   16647 bp

# BciVI.KpnI (GTATCC+GGTACC) double digest, 983 thousand fragments (696093+287974 sites), 3029 bp average fragment length
   5% of sequence in fragments shorter than    1036 bp
  10% of sequence in fragments shorter than    1594 bp
  15% of sequence in fragments shorter than    2067 bp
  20% of sequence in fragments shorter than    2506 bp
  25% of sequence in fragments shorter than    2951 bp
  30% of sequence in fragments shorter than    3394 bp
  35% of sequence in fragments shorter than    3843 bp
  40% of sequence in fragments shorter than    4303 bp
  45% of sequence in fragments shorter than    4786 bp
  50% of sequence in fragments shorter than    5301 bp
  55% of sequence in fragments shorter than    5854 bp
  60% of sequence in fragments shorter than    6458 bp
  65% of sequence in fragments shorter than    7120 bp
  70% of sequence in fragments shorter than    7871 bp
  75% of sequence in fragments shorter than    8755 bp
  80% of sequence in fragments shorter than    9831 bp
  85% of sequence in fragments shorter than   11219 bp
  90% of sequence in fragments shorter than   13200 bp
  95% of sequence in fragments shorter than   16995 bp

# BciVI.NheI (GTATCC+GCTAGC) double digest, 983 thousand fragments (696093+287384 sites), 3031 bp average fragment length
   5% of sequence in fragments shorter than    1061 bp
  10% of sequence in fragments shorter than    1591 bp
  15% of sequence in fragments shorter than    2069 bp
  20% of sequence in fragments shorter than    2508 bp
  25% of sequence in fragments shorter than    2945 bp
  30% of sequence in fragments shorter than    3390 bp
  35% of sequence in fragments shorter than    3841 bp
  40% of sequence in fragments shorter than    4306 bp
  45% of sequence in fragments shorter than    4795 bp
  50% of sequence in fragments shorter than    5309 bp
  55% of sequence in fragments shorter than    5868 bp
  60% of sequence in fragments shorter than    6478 bp
  65% of sequence in fragments shorter than    7151 bp
  70% of sequence in fragments shorter than    7918 bp
  75% of sequence in fragments shorter than    8808 bp
  80% of sequence in fragments shorter than    9902 bp
  85% of sequence in fragments shorter than   11296 bp
  90% of sequence in fragments shorter than   13319 bp
  95% of sequence in fragments shorter than   17101 bp

# SnaBI.XbaI (TACGTA+TCTAGA) double digest, 982 thousand fragments (132299+850998 sites), 3031 bp average fragment length
   5% of sequence in fragments shorter than    1030 bp
  10% of sequence in fragments shorter than    1569 bp
  15% of sequence in fragments shorter than    2056 bp
  20% of sequence in fragments shorter than    2505 bp
  25% of sequence in fragments shorter than    2963 bp
  30% of sequence in fragments shorter than    3418 bp
  35% of sequence in fragments shorter than    3882 bp
  40% of sequence in fragments shorter than    4359 bp
  45% of sequence in fragments shorter than    4856 bp
  50% of sequence in fragments shorter than    5384 bp
  55% of sequence in fragments shorter than    5953 bp
  60% of sequence in fragments shorter than    6569 bp
  65% of sequence in fragments shorter than    7265 bp
  70% of sequence in fragments shorter than    8047 bp
  75% of sequence in fragments shorter than    8969 bp
  80% of sequence in fragments shorter than   10105 bp
  85% of sequence in fragments shorter than   11580 bp
  90% of sequence in fragments shorter than   13692 bp
  95% of sequence in fragments shorter than   17620 bp

# AscI.BspHI (GGCGCGCC+TCATGA) double digest, 982 thousand fragments (4751+978290 sites), 3032 bp average fragment length
   5% of sequence in fragments shorter than    1069 bp
  10% of sequence in fragments shorter than    1627 bp
  15% of sequence in fragments shorter than    2086 bp
  20% of sequence in fragments shorter than    2516 bp
  25% of sequence in fragments shorter than    2950 bp
  30% of sequence in fragments shorter than    3378 bp
  35% of sequence in fragments shorter than    3821 bp
  40% of sequence in fragments shorter than    4274 bp
  45% of sequence in fragments shorter than    4749 bp
  50% of sequence in fragments shorter than    5253 bp
  55% of sequence in fragments shorter than    5794 bp
  60% of sequence in fragments shorter than    6390 bp
  65% of sequence in fragments shorter than    7042 bp
  70% of sequence in fragments shorter than    7803 bp
  75% of sequence in fragments shorter than    8686 bp
  80% of sequence in fragments shorter than    9773 bp
  85% of sequence in fragments shorter than   11213 bp
  90% of sequence in fragments shorter than   13334 bp
  95% of sequence in fragments shorter than   17713 bp

# HindIII.XhoI (AAGCTT+CTCGAG) double digest, 981 thousand fragments (860365+121308 sites), 3037 bp average fragment length
   5% of sequence in fragments shorter than    1068 bp
  10% of sequence in fragments shorter than    1624 bp
  15% of sequence in fragments shorter than    2063 bp
  20% of sequence in fragments shorter than    2514 bp
  25% of sequence in fragments shorter than    2945 bp
  30% of sequence in fragments shorter than    3383 bp
  35% of sequence in fragments shorter than    3824 bp
  40% of sequence in fragments shorter than    4279 bp
  45% of sequence in fragments shorter than    4750 bp
  50% of sequence in fragments shorter than    5249 bp
  55% of sequence in fragments shorter than    5787 bp
  60% of sequence in fragments shorter than    6379 bp
  65% of sequence in fragments shorter than    7025 bp
  70% of sequence in fragments shorter than    7763 bp
  75% of sequence in fragments shorter than    8615 bp
  80% of sequence in fragments shorter than    9649 bp
  85% of sequence in fragments shorter than   10991 bp
  90% of sequence in fragments shorter than   12862 bp
  95% of sequence in fragments shorter than   16435 bp

# SacI.SapI (GAGCTC+GCTCTTC) double digest, 980 thousand fragments (603323+377161 sites), 3040 bp average fragment length
   5% of sequence in fragments shorter than    1050 bp
  10% of sequence in fragments shorter than    1627 bp
  15% of sequence in fragments shorter than    2131 bp
  20% of sequence in fragments shorter than    2614 bp
  25% of sequence in fragments shorter than    3100 bp
  30% of sequence in fragments shorter than    3582 bp
  35% of sequence in fragments shorter than    4079 bp
  40% of sequence in fragments shorter than    4584 bp
  45% of sequence in fragments shorter than    5121 bp
  50% of sequence in fragments shorter than    5693 bp
  55% of sequence in fragments shorter than    6311 bp
  60% of sequence in fragments shorter than    6998 bp
  65% of sequence in fragments shorter than    7761 bp
  70% of sequence in fragments shorter than    8623 bp
  75% of sequence in fragments shorter than    9654 bp
  80% of sequence in fragments shorter than   10903 bp
  85% of sequence in fragments shorter than   12532 bp
  90% of sequence in fragments shorter than   14951 bp
  95% of sequence in fragments shorter than   19555 bp

# SacI.SmaI (GAGCTC+CCCGGG) double digest, 979 thousand fragments (603323+376940 sites), 3041 bp average fragment length
   5% of sequence in fragments shorter than     999 bp
  10% of sequence in fragments shorter than    1569 bp
  15% of sequence in fragments shorter than    2086 bp
  20% of sequence in fragments shorter than    2586 bp
  25% of sequence in fragments shorter than    3100 bp
  30% of sequence in fragments shorter than    3625 bp
  35% of sequence in fragments shorter than    4174 bp
  40% of sequence in fragments shorter than    4749 bp
  45% of sequence in fragments shorter than    5362 bp
  50% of sequence in fragments shorter than    6023 bp
  55% of sequence in fragments shorter than    6747 bp
  60% of sequence in fragments shorter than    7549 bp
  65% of sequence in fragments shorter than    8447 bp
  70% of sequence in fragments shorter than    9499 bp
  75% of sequence in fragments shorter than   10754 bp
  80% of sequence in fragments shorter than   12306 bp
  85% of sequence in fragments shorter than   14347 bp
  90% of sequence in fragments shorter than   17380 bp
  95% of sequence in fragments shorter than   23506 bp

# EcoRI.SnaBI (GAATTC+TACGTA) double digest, 979 thousand fragments (847401+132299 sites), 3043 bp average fragment length
   5% of sequence in fragments shorter than    1036 bp
  10% of sequence in fragments shorter than    1623 bp
  15% of sequence in fragments shorter than    2137 bp
  20% of sequence in fragments shorter than    2607 bp
  25% of sequence in fragments shorter than    3057 bp
  30% of sequence in fragments shorter than    3502 bp
  35% of sequence in fragments shorter than    3968 bp
  40% of sequence in fragments shorter than    4444 bp
  45% of sequence in fragments shorter than    4948 bp
  50% of sequence in fragments shorter than    5482 bp
  55% of sequence in fragments shorter than    6048 bp
  60% of sequence in fragments shorter than    6677 bp
  65% of sequence in fragments shorter than    7375 bp
  70% of sequence in fragments shorter than    8159 bp
  75% of sequence in fragments shorter than    9066 bp
  80% of sequence in fragments shorter than   10177 bp
  85% of sequence in fragments shorter than   11600 bp
  90% of sequence in fragments shorter than   13622 bp
  95% of sequence in fragments shorter than   17275 bp

# AflII.BstEII (CTTAAG+GGTNACC) double digest, 978 thousand fragments (643284+335865 sites), 3044 bp average fragment length
   5% of sequence in fragments shorter than    1077 bp
  10% of sequence in fragments shorter than    1629 bp
  15% of sequence in fragments shorter than    2100 bp
  20% of sequence in fragments shorter than    2528 bp
  25% of sequence in fragments shorter than    2908 bp
  30% of sequence in fragments shorter than    3333 bp
  35% of sequence in fragments shorter than    3760 bp
  40% of sequence in fragments shorter than    4205 bp
  45% of sequence in fragments shorter than    4670 bp
  50% of sequence in fragments shorter than    5156 bp
  55% of sequence in fragments shorter than    5681 bp
  60% of sequence in fragments shorter than    6252 bp
  65% of sequence in fragments shorter than    6894 bp
  70% of sequence in fragments shorter than    7619 bp
  75% of sequence in fragments shorter than    8459 bp
  80% of sequence in fragments shorter than    9494 bp
  85% of sequence in fragments shorter than   10857 bp
  90% of sequence in fragments shorter than   12845 bp
  95% of sequence in fragments shorter than   16953 bp

# AgeI.StuI (ACCGGT+AGGCCT) double digest, 978 thousand fragments (53386+925729 sites), 3044 bp average fragment length
   5% of sequence in fragments shorter than     911 bp
  10% of sequence in fragments shorter than    1552 bp
  15% of sequence in fragments shorter than    2128 bp
  20% of sequence in fragments shorter than    2721 bp
  25% of sequence in fragments shorter than    3292 bp
  30% of sequence in fragments shorter than    3864 bp
  35% of sequence in fragments shorter than    4454 bp
  40% of sequence in fragments shorter than    5068 bp
  45% of sequence in fragments shorter than    5713 bp
  50% of sequence in fragments shorter than    6396 bp
  55% of sequence in fragments shorter than    7142 bp
  60% of sequence in fragments shorter than    7945 bp
  65% of sequence in fragments shorter than    8838 bp
  70% of sequence in fragments shorter than    9857 bp
  75% of sequence in fragments shorter than   11046 bp
  80% of sequence in fragments shorter than   12446 bp
  85% of sequence in fragments shorter than   14238 bp
  90% of sequence in fragments shorter than   16766 bp
  95% of sequence in fragments shorter than   21115 bp

# NaeI.XbaI (GCCGGC+TCTAGA) double digest, 976 thousand fragments (125692+850998 sites), 3052 bp average fragment length
   5% of sequence in fragments shorter than    1038 bp
  10% of sequence in fragments shorter than    1597 bp
  15% of sequence in fragments shorter than    2090 bp
  20% of sequence in fragments shorter than    2545 bp
  25% of sequence in fragments shorter than    3003 bp
  30% of sequence in fragments shorter than    3462 bp
  35% of sequence in fragments shorter than    3920 bp
  40% of sequence in fragments shorter than    4394 bp
  45% of sequence in fragments shorter than    4886 bp
  50% of sequence in fragments shorter than    5405 bp
  55% of sequence in fragments shorter than    5965 bp
  60% of sequence in fragments shorter than    6566 bp
  65% of sequence in fragments shorter than    7231 bp
  70% of sequence in fragments shorter than    7971 bp
  75% of sequence in fragments shorter than    8830 bp
  80% of sequence in fragments shorter than    9885 bp
  85% of sequence in fragments shorter than   11245 bp
  90% of sequence in fragments shorter than   13091 bp
  95% of sequence in fragments shorter than   16382 bp

# AvaIII.PmeI (ATGCAT+GTTTAAAC) double digest, 975 thousand fragments (933357+42094 sites), 3056 bp average fragment length
   5% of sequence in fragments shorter than    1077 bp
  10% of sequence in fragments shorter than    1643 bp
  15% of sequence in fragments shorter than    2124 bp
  20% of sequence in fragments shorter than    2587 bp
  25% of sequence in fragments shorter than    3042 bp
  30% of sequence in fragments shorter than    3500 bp
  35% of sequence in fragments shorter than    3974 bp
  40% of sequence in fragments shorter than    4470 bp
  45% of sequence in fragments shorter than    4988 bp
  50% of sequence in fragments shorter than    5548 bp
  55% of sequence in fragments shorter than    6154 bp
  60% of sequence in fragments shorter than    6824 bp
  65% of sequence in fragments shorter than    7574 bp
  70% of sequence in fragments shorter than    8430 bp
  75% of sequence in fragments shorter than    9448 bp
  80% of sequence in fragments shorter than   10740 bp
  85% of sequence in fragments shorter than   12407 bp
  90% of sequence in fragments shorter than   14919 bp
  95% of sequence in fragments shorter than   20183 bp

# AatII.NdeI (GACGTC+CATATG) double digest, 974 thousand fragments (71089+903360 sites), 3059 bp average fragment length
   5% of sequence in fragments shorter than    1042 bp
  10% of sequence in fragments shorter than    1587 bp
  15% of sequence in fragments shorter than    2102 bp
  20% of sequence in fragments shorter than    2592 bp
  25% of sequence in fragments shorter than    3063 bp
  30% of sequence in fragments shorter than    3536 bp
  35% of sequence in fragments shorter than    4023 bp
  40% of sequence in fragments shorter than    4522 bp
  45% of sequence in fragments shorter than    5039 bp
  50% of sequence in fragments shorter than    5585 bp
  55% of sequence in fragments shorter than    6174 bp
  60% of sequence in fragments shorter than    6826 bp
  65% of sequence in fragments shorter than    7560 bp
  70% of sequence in fragments shorter than    8405 bp
  75% of sequence in fragments shorter than    9413 bp
  80% of sequence in fragments shorter than   10655 bp
  85% of sequence in fragments shorter than   12321 bp
  90% of sequence in fragments shorter than   14831 bp
  95% of sequence in fragments shorter than   20096 bp

# BclI.BsrBI (TGATCA+CCGCTC) double digest, 973 thousand fragments (738785+235523 sites), 3059 bp average fragment length
   5% of sequence in fragments shorter than    1068 bp
  10% of sequence in fragments shorter than    1632 bp
  15% of sequence in fragments shorter than    2107 bp
  20% of sequence in fragments shorter than    2560 bp
  25% of sequence in fragments shorter than    2999 bp
  30% of sequence in fragments shorter than    3436 bp
  35% of sequence in fragments shorter than    3886 bp
  40% of sequence in fragments shorter than    4338 bp
  45% of sequence in fragments shorter than    4817 bp
  50% of sequence in fragments shorter than    5319 bp
  55% of sequence in fragments shorter than    5864 bp
  60% of sequence in fragments shorter than    6459 bp
  65% of sequence in fragments shorter than    7114 bp
  70% of sequence in fragments shorter than    7862 bp
  75% of sequence in fragments shorter than    8718 bp
  80% of sequence in fragments shorter than    9768 bp
  85% of sequence in fragments shorter than   11127 bp
  90% of sequence in fragments shorter than   13066 bp
  95% of sequence in fragments shorter than   16737 bp

# EcoRI.NaeI (GAATTC+GCCGGC) double digest, 972 thousand fragments (847401+125692 sites), 3063 bp average fragment length
   5% of sequence in fragments shorter than    1042 bp
  10% of sequence in fragments shorter than    1656 bp
  15% of sequence in fragments shorter than    2187 bp
  20% of sequence in fragments shorter than    2671 bp
  25% of sequence in fragments shorter than    3136 bp
  30% of sequence in fragments shorter than    3589 bp
  35% of sequence in fragments shorter than    4062 bp
  40% of sequence in fragments shorter than    4541 bp
  45% of sequence in fragments shorter than    5051 bp
  50% of sequence in fragments shorter than    5583 bp
  55% of sequence in fragments shorter than    6149 bp
  60% of sequence in fragments shorter than    6766 bp
  65% of sequence in fragments shorter than    7454 bp
  70% of sequence in fragments shorter than    8217 bp
  75% of sequence in fragments shorter than    9093 bp
  80% of sequence in fragments shorter than   10152 bp
  85% of sequence in fragments shorter than   11513 bp
  90% of sequence in fragments shorter than   13386 bp
  95% of sequence in fragments shorter than   16610 bp

# XbaI.XhoI (TCTAGA+CTCGAG) double digest, 971 thousand fragments (850998+121308 sites), 3066 bp average fragment length
   5% of sequence in fragments shorter than    1043 bp
  10% of sequence in fragments shorter than    1593 bp
  15% of sequence in fragments shorter than    2056 bp
  20% of sequence in fragments shorter than    2500 bp
  25% of sequence in fragments shorter than    2953 bp
  30% of sequence in fragments shorter than    3405 bp
  35% of sequence in fragments shorter than    3864 bp
  40% of sequence in fragments shorter than    4335 bp
  45% of sequence in fragments shorter than    4828 bp
  50% of sequence in fragments shorter than    5344 bp
  55% of sequence in fragments shorter than    5896 bp
  60% of sequence in fragments shorter than    6496 bp
  65% of sequence in fragments shorter than    7162 bp
  70% of sequence in fragments shorter than    7911 bp
  75% of sequence in fragments shorter than    8773 bp
  80% of sequence in fragments shorter than    9822 bp
  85% of sequence in fragments shorter than   11192 bp
  90% of sequence in fragments shorter than   13073 bp
  95% of sequence in fragments shorter than   16392 bp

# HgaI.SpeI (GACGC+ACTAGT) double digest, 971 thousand fragments (571889+400286 sites), 3066 bp average fragment length
   5% of sequence in fragments shorter than    1074 bp
  10% of sequence in fragments shorter than    1680 bp
  15% of sequence in fragments shorter than    2197 bp
  20% of sequence in fragments shorter than    2676 bp
  25% of sequence in fragments shorter than    3162 bp
  30% of sequence in fragments shorter than    3656 bp
  35% of sequence in fragments shorter than    4157 bp
  40% of sequence in fragments shorter than    4682 bp
  45% of sequence in fragments shorter than    5227 bp
  50% of sequence in fragments shorter than    5790 bp
  55% of sequence in fragments shorter than    6409 bp
  60% of sequence in fragments shorter than    7078 bp
  65% of sequence in fragments shorter than    7830 bp
  70% of sequence in fragments shorter than    8677 bp
  75% of sequence in fragments shorter than    9676 bp
  80% of sequence in fragments shorter than   10897 bp
  85% of sequence in fragments shorter than   12428 bp
  90% of sequence in fragments shorter than   14675 bp
  95% of sequence in fragments shorter than   18754 bp

# AvrII.SapI (CCTAGG+GCTCTTC) double digest, 971 thousand fragments (594956+377161 sites), 3066 bp average fragment length
   5% of sequence in fragments shorter than    1051 bp
  10% of sequence in fragments shorter than    1616 bp
  15% of sequence in fragments shorter than    2072 bp
  20% of sequence in fragments shorter than    2530 bp
  25% of sequence in fragments shorter than    2960 bp
  30% of sequence in fragments shorter than    3411 bp
  35% of sequence in fragments shorter than    3864 bp
  40% of sequence in fragments shorter than    4335 bp
  45% of sequence in fragments shorter than    4831 bp
  50% of sequence in fragments shorter than    5354 bp
  55% of sequence in fragments shorter than    5920 bp
  60% of sequence in fragments shorter than    6543 bp
  65% of sequence in fragments shorter than    7244 bp
  70% of sequence in fragments shorter than    8028 bp
  75% of sequence in fragments shorter than    8952 bp
  80% of sequence in fragments shorter than   10080 bp
  85% of sequence in fragments shorter than   11570 bp
  90% of sequence in fragments shorter than   13785 bp
  95% of sequence in fragments shorter than   18262 bp

# AvrII.SmaI (CCTAGG+CCCGGG) double digest, 971 thousand fragments (594956+376940 sites), 3067 bp average fragment length
   5% of sequence in fragments shorter than    1008 bp
  10% of sequence in fragments shorter than    1596 bp
  15% of sequence in fragments shorter than    2116 bp
  20% of sequence in fragments shorter than    2614 bp
  25% of sequence in fragments shorter than    3110 bp
  30% of sequence in fragments shorter than    3611 bp
  35% of sequence in fragments shorter than    4121 bp
  40% of sequence in fragments shorter than    4648 bp
  45% of sequence in fragments shorter than    5211 bp
  50% of sequence in fragments shorter than    5820 bp
  55% of sequence in fragments shorter than    6482 bp
  60% of sequence in fragments shorter than    7208 bp
  65% of sequence in fragments shorter than    8035 bp
  70% of sequence in fragments shorter than    8975 bp
  75% of sequence in fragments shorter than   10094 bp
  80% of sequence in fragments shorter than   11490 bp
  85% of sequence in fragments shorter than   13352 bp
  90% of sequence in fragments shorter than   16116 bp
  95% of sequence in fragments shorter than   21942 bp

# EciI.SpeI (GGCGGA+ACTAGT) double digest, 971 thousand fragments (571273+400286 sites), 3068 bp average fragment length
   5% of sequence in fragments shorter than    1052 bp
  10% of sequence in fragments shorter than    1635 bp
  15% of sequence in fragments shorter than    2142 bp
  20% of sequence in fragments shorter than    2630 bp
  25% of sequence in fragments shorter than    3113 bp
  30% of sequence in fragments shorter than    3606 bp
  35% of sequence in fragments shorter than    4108 bp
  40% of sequence in fragments shorter than    4640 bp
  45% of sequence in fragments shorter than    5191 bp
  50% of sequence in fragments shorter than    5782 bp
  55% of sequence in fragments shorter than    6421 bp
  60% of sequence in fragments shorter than    7112 bp
  65% of sequence in fragments shorter than    7889 bp
  70% of sequence in fragments shorter than    8773 bp
  75% of sequence in fragments shorter than    9819 bp
  80% of sequence in fragments shorter than   11114 bp
  85% of sequence in fragments shorter than   12756 bp
  90% of sequence in fragments shorter than   15198 bp
  95% of sequence in fragments shorter than   19926 bp

# NdeI.SacII (CATATG+CCGCGG) double digest, 969 thousand fragments (903360+66901 sites), 3072 bp average fragment length
   5% of sequence in fragments shorter than    1049 bp
  10% of sequence in fragments shorter than    1612 bp
  15% of sequence in fragments shorter than    2145 bp
  20% of sequence in fragments shorter than    2648 bp
  25% of sequence in fragments shorter than    3132 bp
  30% of sequence in fragments shorter than    3623 bp
  35% of sequence in fragments shorter than    4125 bp
  40% of sequence in fragments shorter than    4636 bp
  45% of sequence in fragments shorter than    5160 bp
  50% of sequence in fragments shorter than    5718 bp
  55% of sequence in fragments shorter than    6322 bp
  60% of sequence in fragments shorter than    6986 bp
  65% of sequence in fragments shorter than    7738 bp
  70% of sequence in fragments shorter than    8599 bp
  75% of sequence in fragments shorter than    9615 bp
  80% of sequence in fragments shorter than   10869 bp
  85% of sequence in fragments shorter than   12546 bp
  90% of sequence in fragments shorter than   15054 bp
  95% of sequence in fragments shorter than   20286 bp

# BsrBI.FnuDII (CCGCTC+CGCG) double digest, 969 thousand fragments (235523+733940 sites), 3075 bp average fragment length
   5% of sequence in fragments shorter than    1275 bp
  10% of sequence in fragments shorter than    2271 bp
  15% of sequence in fragments shorter than    3201 bp
  20% of sequence in fragments shorter than    4167 bp
  25% of sequence in fragments shorter than    5187 bp
  30% of sequence in fragments shorter than    6252 bp
  35% of sequence in fragments shorter than    7397 bp
  40% of sequence in fragments shorter than    8625 bp
  45% of sequence in fragments shorter than    9969 bp
  50% of sequence in fragments shorter than   11447 bp
  55% of sequence in fragments shorter than   13089 bp
  60% of sequence in fragments shorter than   14959 bp
  65% of sequence in fragments shorter than   17079 bp
  70% of sequence in fragments shorter than   19534 bp
  75% of sequence in fragments shorter than   22457 bp
  80% of sequence in fragments shorter than   26141 bp
  85% of sequence in fragments shorter than   30863 bp
  90% of sequence in fragments shorter than   37780 bp
  95% of sequence in fragments shorter than   50748 bp

# BamHI.SacI (GGATCC+GAGCTC) double digest, 968 thousand fragments (365999+603323 sites), 3075 bp average fragment length
   5% of sequence in fragments shorter than    1056 bp
  10% of sequence in fragments shorter than    1612 bp
  15% of sequence in fragments shorter than    2111 bp
  20% of sequence in fragments shorter than    2585 bp
  25% of sequence in fragments shorter than    3025 bp
  30% of sequence in fragments shorter than    3475 bp
  35% of sequence in fragments shorter than    3967 bp
  40% of sequence in fragments shorter than    4478 bp
  45% of sequence in fragments shorter than    5013 bp
  50% of sequence in fragments shorter than    5586 bp
  55% of sequence in fragments shorter than    6209 bp
  60% of sequence in fragments shorter than    6893 bp
  65% of sequence in fragments shorter than    7668 bp
  70% of sequence in fragments shorter than    8539 bp
  75% of sequence in fragments shorter than    9571 bp
  80% of sequence in fragments shorter than   10831 bp
  85% of sequence in fragments shorter than   12495 bp
  90% of sequence in fragments shorter than   14896 bp
  95% of sequence in fragments shorter than   19508 bp

# BclI.KasI (TGATCA+GGCGCC) double digest, 968 thousand fragments (738785+230381 sites), 3075 bp average fragment length
   5% of sequence in fragments shorter than    1072 bp
  10% of sequence in fragments shorter than    1632 bp
  15% of sequence in fragments shorter than    2111 bp
  20% of sequence in fragments shorter than    2558 bp
  25% of sequence in fragments shorter than    2989 bp
  30% of sequence in fragments shorter than    3407 bp
  35% of sequence in fragments shorter than    3853 bp
  40% of sequence in fragments shorter than    4305 bp
  45% of sequence in fragments shorter than    4782 bp
  50% of sequence in fragments shorter than    5284 bp
  55% of sequence in fragments shorter than    5827 bp
  60% of sequence in fragments shorter than    6422 bp
  65% of sequence in fragments shorter than    7075 bp
  70% of sequence in fragments shorter than    7818 bp
  75% of sequence in fragments shorter than    8686 bp
  80% of sequence in fragments shorter than    9741 bp
  85% of sequence in fragments shorter than   11142 bp
  90% of sequence in fragments shorter than   13194 bp
  95% of sequence in fragments shorter than   17227 bp

# EcoRI.XhoI (GAATTC+CTCGAG) double digest, 968 thousand fragments (847401+121308 sites), 3077 bp average fragment length
   5% of sequence in fragments shorter than    1049 bp
  10% of sequence in fragments shorter than    1648 bp
  15% of sequence in fragments shorter than    2145 bp
  20% of sequence in fragments shorter than    2621 bp
  25% of sequence in fragments shorter than    3075 bp
  30% of sequence in fragments shorter than    3530 bp
  35% of sequence in fragments shorter than    3997 bp
  40% of sequence in fragments shorter than    4475 bp
  45% of sequence in fragments shorter than    4976 bp
  50% of sequence in fragments shorter than    5502 bp
  55% of sequence in fragments shorter than    6053 bp
  60% of sequence in fragments shorter than    6664 bp
  65% of sequence in fragments shorter than    7345 bp
  70% of sequence in fragments shorter than    8093 bp
  75% of sequence in fragments shorter than    8970 bp
  80% of sequence in fragments shorter than   10024 bp
  85% of sequence in fragments shorter than   11373 bp
  90% of sequence in fragments shorter than   13284 bp
  95% of sequence in fragments shorter than   16539 bp

# PmeI.StuI (GTTTAAAC+AGGCCT) double digest, 967 thousand fragments (42094+925729 sites), 3080 bp average fragment length
   5% of sequence in fragments shorter than     923 bp
  10% of sequence in fragments shorter than    1574 bp
  15% of sequence in fragments shorter than    2154 bp
  20% of sequence in fragments shorter than    2746 bp
  25% of sequence in fragments shorter than    3313 bp
  30% of sequence in fragments shorter than    3883 bp
  35% of sequence in fragments shorter than    4464 bp
  40% of sequence in fragments shorter than    5071 bp
  45% of sequence in fragments shorter than    5710 bp
  50% of sequence in fragments shorter than    6381 bp
  55% of sequence in fragments shorter than    7120 bp
  60% of sequence in fragments shorter than    7910 bp
  65% of sequence in fragments shorter than    8786 bp
  70% of sequence in fragments shorter than    9789 bp
  75% of sequence in fragments shorter than   10960 bp
  80% of sequence in fragments shorter than   12343 bp
  85% of sequence in fragments shorter than   14107 bp
  90% of sequence in fragments shorter than   16538 bp
  95% of sequence in fragments shorter than   20797 bp

# BstEII.HaeII (GGTNACC+RGCGCY) double digest, 965 thousand fragments (335865+630101 sites), 3086 bp average fragment length
   5% of sequence in fragments shorter than     940 bp
  10% of sequence in fragments shorter than    1629 bp
  15% of sequence in fragments shorter than    2258 bp
  20% of sequence in fragments shorter than    2893 bp
  25% of sequence in fragments shorter than    3513 bp
  30% of sequence in fragments shorter than    4155 bp
  35% of sequence in fragments shorter than    4817 bp
  40% of sequence in fragments shorter than    5505 bp
  45% of sequence in fragments shorter than    6230 bp
  50% of sequence in fragments shorter than    7005 bp
  55% of sequence in fragments shorter than    7864 bp
  60% of sequence in fragments shorter than    8799 bp
  65% of sequence in fragments shorter than    9846 bp
  70% of sequence in fragments shorter than   11048 bp
  75% of sequence in fragments shorter than   12460 bp
  80% of sequence in fragments shorter than   14170 bp
  85% of sequence in fragments shorter than   16384 bp
  90% of sequence in fragments shorter than   19497 bp
  95% of sequence in fragments shorter than   24843 bp

# BclI.SwaI (TGATCA+ATTTAAAT) double digest, 965 thousand fragments (738785+226602 sites), 3087 bp average fragment length
   5% of sequence in fragments shorter than    1077 bp
  10% of sequence in fragments shorter than    1627 bp
  15% of sequence in fragments shorter than    2101 bp
  20% of sequence in fragments shorter than    2546 bp
  25% of sequence in fragments shorter than    2978 bp
  30% of sequence in fragments shorter than    3415 bp
  35% of sequence in fragments shorter than    3865 bp
  40% of sequence in fragments shorter than    4323 bp
  45% of sequence in fragments shorter than    4810 bp
  50% of sequence in fragments shorter than    5323 bp
  55% of sequence in fragments shorter than    5879 bp
  60% of sequence in fragments shorter than    6496 bp
  65% of sequence in fragments shorter than    7183 bp
  70% of sequence in fragments shorter than    7970 bp
  75% of sequence in fragments shorter than    8901 bp
  80% of sequence in fragments shorter than   10053 bp
  85% of sequence in fragments shorter than   11599 bp
  90% of sequence in fragments shorter than   13919 bp
  95% of sequence in fragments shorter than   19093 bp

# AvaIII.SalI (ATGCAT+GTCGAC) double digest, 964 thousand fragments (933357+31396 sites), 3090 bp average fragment length
   5% of sequence in fragments shorter than    1094 bp
  10% of sequence in fragments shorter than    1665 bp
  15% of sequence in fragments shorter than    2153 bp
  20% of sequence in fragments shorter than    2617 bp
  25% of sequence in fragments shorter than    3075 bp
  30% of sequence in fragments shorter than    3536 bp
  35% of sequence in fragments shorter than    4013 bp
  40% of sequence in fragments shorter than    4511 bp
  45% of sequence in fragments shorter than    5029 bp
  50% of sequence in fragments shorter than    5589 bp
  55% of sequence in fragments shorter than    6194 bp
  60% of sequence in fragments shorter than    6859 bp
  65% of sequence in fragments shorter than    7607 bp
  70% of sequence in fragments shorter than    8456 bp
  75% of sequence in fragments shorter than    9461 bp
  80% of sequence in fragments shorter than   10722 bp
  85% of sequence in fragments shorter than   12360 bp
  90% of sequence in fragments shorter than   14810 bp
  95% of sequence in fragments shorter than   19845 bp

# MfeI.SpeI (CAATTG+ACTAGT) double digest, 964 thousand fragments (564303+400286 sites), 3090 bp average fragment length
   5% of sequence in fragments shorter than    1059 bp
  10% of sequence in fragments shorter than    1581 bp
  15% of sequence in fragments shorter than    2065 bp
  20% of sequence in fragments shorter than    2515 bp
  25% of sequence in fragments shorter than    2965 bp
  30% of sequence in fragments shorter than    3418 bp
  35% of sequence in fragments shorter than    3884 bp
  40% of sequence in fragments shorter than    4365 bp
  45% of sequence in fragments shorter than    4863 bp
  50% of sequence in fragments shorter than    5398 bp
  55% of sequence in fragments shorter than    5982 bp
  60% of sequence in fragments shorter than    6627 bp
  65% of sequence in fragments shorter than    7357 bp
  70% of sequence in fragments shorter than    8211 bp
  75% of sequence in fragments shorter than    9225 bp
  80% of sequence in fragments shorter than   10522 bp
  85% of sequence in fragments shorter than   12251 bp
  90% of sequence in fragments shorter than   14943 bp
  95% of sequence in fragments shorter than   20686 bp

# FnuDII.KasI (CGCG+GGCGCC) double digest, 963 thousand fragments (733940+230381 sites), 3091 bp average fragment length
   5% of sequence in fragments shorter than    1303 bp
  10% of sequence in fragments shorter than    2374 bp
  15% of sequence in fragments shorter than    3392 bp
  20% of sequence in fragments shorter than    4446 bp
  25% of sequence in fragments shorter than    5553 bp
  30% of sequence in fragments shorter than    6724 bp
  35% of sequence in fragments shorter than    7999 bp
  40% of sequence in fragments shorter than    9367 bp
  45% of sequence in fragments shorter than   10869 bp
  50% of sequence in fragments shorter than   12546 bp
  55% of sequence in fragments shorter than   14430 bp
  60% of sequence in fragments shorter than   16593 bp
  65% of sequence in fragments shorter than   18970 bp
  70% of sequence in fragments shorter than   21759 bp
  75% of sequence in fragments shorter than   25209 bp
  80% of sequence in fragments shorter than   29418 bp
  85% of sequence in fragments shorter than   35092 bp
  90% of sequence in fragments shorter than   43341 bp
  95% of sequence in fragments shorter than   59562 bp

# NdeI.PspXI (CATATG+VCTCGAGB) double digest, 962 thousand fragments (903360+59193 sites), 3097 bp average fragment length
   5% of sequence in fragments shorter than    1054 bp
  10% of sequence in fragments shorter than    1605 bp
  15% of sequence in fragments shorter than    2128 bp
  20% of sequence in fragments shorter than    2622 bp
  25% of sequence in fragments shorter than    3100 bp
  30% of sequence in fragments shorter than    3579 bp
  35% of sequence in fragments shorter than    4072 bp
  40% of sequence in fragments shorter than    4576 bp
  45% of sequence in fragments shorter than    5095 bp
  50% of sequence in fragments shorter than    5650 bp
  55% of sequence in fragments shorter than    6248 bp
  60% of sequence in fragments shorter than    6907 bp
  65% of sequence in fragments shorter than    7653 bp
  70% of sequence in fragments shorter than    8513 bp
  75% of sequence in fragments shorter than    9531 bp
  80% of sequence in fragments shorter than   10805 bp
  85% of sequence in fragments shorter than   12460 bp
  90% of sequence in fragments shorter than   15004 bp
  95% of sequence in fragments shorter than   20372 bp

# HgaI.HpaI (GACGC+GTTAAC) double digest, 960 thousand fragments (571889+389418 sites), 3101 bp average fragment length
   5% of sequence in fragments shorter than    1071 bp
  10% of sequence in fragments shorter than    1691 bp
  15% of sequence in fragments shorter than    2233 bp
  20% of sequence in fragments shorter than    2747 bp
  25% of sequence in fragments shorter than    3245 bp
  30% of sequence in fragments shorter than    3753 bp
  35% of sequence in fragments shorter than    4266 bp
  40% of sequence in fragments shorter than    4807 bp
  45% of sequence in fragments shorter than    5380 bp
  50% of sequence in fragments shorter than    5980 bp
  55% of sequence in fragments shorter than    6622 bp
  60% of sequence in fragments shorter than    7312 bp
  65% of sequence in fragments shorter than    8098 bp
  70% of sequence in fragments shorter than    8984 bp
  75% of sequence in fragments shorter than   10025 bp
  80% of sequence in fragments shorter than   11324 bp
  85% of sequence in fragments shorter than   12980 bp
  90% of sequence in fragments shorter than   15387 bp
  95% of sequence in fragments shorter than   19846 bp

# AvrII.BamHI (CCTAGG+GGATCC) double digest, 960 thousand fragments (594956+365999 sites), 3102 bp average fragment length
   5% of sequence in fragments shorter than    1081 bp
  10% of sequence in fragments shorter than    1671 bp
  15% of sequence in fragments shorter than    2174 bp
  20% of sequence in fragments shorter than    2657 bp
  25% of sequence in fragments shorter than    3125 bp
  30% of sequence in fragments shorter than    3594 bp
  35% of sequence in fragments shorter than    4072 bp
  40% of sequence in fragments shorter than    4563 bp
  45% of sequence in fragments shorter than    5076 bp
  50% of sequence in fragments shorter than    5617 bp
  55% of sequence in fragments shorter than    6203 bp
  60% of sequence in fragments shorter than    6853 bp
  65% of sequence in fragments shorter than    7578 bp
  70% of sequence in fragments shorter than    8398 bp
  75% of sequence in fragments shorter than    9386 bp
  80% of sequence in fragments shorter than   10570 bp
  85% of sequence in fragments shorter than   12139 bp
  90% of sequence in fragments shorter than   14445 bp
  95% of sequence in fragments shorter than   19050 bp

# EciI.HpaI (GGCGGA+GTTAAC) double digest, 960 thousand fragments (571273+389418 sites), 3103 bp average fragment length
   5% of sequence in fragments shorter than    1052 bp
  10% of sequence in fragments shorter than    1650 bp
  15% of sequence in fragments shorter than    2179 bp
  20% of sequence in fragments shorter than    2683 bp
  25% of sequence in fragments shorter than    3182 bp
  30% of sequence in fragments shorter than    3683 bp
  35% of sequence in fragments shorter than    4205 bp
  40% of sequence in fragments shorter than    4758 bp
  45% of sequence in fragments shorter than    5340 bp
  50% of sequence in fragments shorter than    5963 bp
  55% of sequence in fragments shorter than    6645 bp
  60% of sequence in fragments shorter than    7378 bp
  65% of sequence in fragments shorter than    8226 bp
  70% of sequence in fragments shorter than    9198 bp
  75% of sequence in fragments shorter than   10338 bp
  80% of sequence in fragments shorter than   11752 bp
  85% of sequence in fragments shorter than   13613 bp
  90% of sequence in fragments shorter than   16353 bp
  95% of sequence in fragments shorter than   21851 bp

# FnuDII.SwaI (CGCG+ATTTAAAT) double digest, 960 thousand fragments (733940+226602 sites), 3103 bp average fragment length
   5% of sequence in fragments shorter than    1314 bp
  10% of sequence in fragments shorter than    2208 bp
  15% of sequence in fragments shorter than    2997 bp
  20% of sequence in fragments shorter than    3745 bp
  25% of sequence in fragments shorter than    4501 bp
  30% of sequence in fragments shorter than    5261 bp
  35% of sequence in fragments shorter than    6053 bp
  40% of sequence in fragments shorter than    6880 bp
  45% of sequence in fragments shorter than    7763 bp
  50% of sequence in fragments shorter than    8707 bp
  55% of sequence in fragments shorter than    9711 bp
  60% of sequence in fragments shorter than   10829 bp
  65% of sequence in fragments shorter than   12100 bp
  70% of sequence in fragments shorter than   13550 bp
  75% of sequence in fragments shorter than   15264 bp
  80% of sequence in fragments shorter than   17408 bp
  85% of sequence in fragments shorter than   20194 bp
  90% of sequence in fragments shorter than   24316 bp
  95% of sequence in fragments shorter than   32675 bp

# SalI.StuI (GTCGAC+AGGCCT) double digest, 956 thousand fragments (31396+925729 sites), 3114 bp average fragment length
   5% of sequence in fragments shorter than     930 bp
  10% of sequence in fragments shorter than    1587 bp
  15% of sequence in fragments shorter than    2179 bp
  20% of sequence in fragments shorter than    2780 bp
  25% of sequence in fragments shorter than    3368 bp
  30% of sequence in fragments shorter than    3953 bp
  35% of sequence in fragments shorter than    4553 bp
  40% of sequence in fragments shorter than    5183 bp
  45% of sequence in fragments shorter than    5844 bp
  50% of sequence in fragments shorter than    6545 bp
  55% of sequence in fragments shorter than    7309 bp
  60% of sequence in fragments shorter than    8124 bp
  65% of sequence in fragments shorter than    9035 bp
  70% of sequence in fragments shorter than   10074 bp
  75% of sequence in fragments shorter than   11288 bp
  80% of sequence in fragments shorter than   12723 bp
  85% of sequence in fragments shorter than   14573 bp
  90% of sequence in fragments shorter than   17123 bp
  95% of sequence in fragments shorter than   21567 bp

# AgeI.NdeI (ACCGGT+CATATG) double digest, 956 thousand fragments (53386+903360 sites), 3116 bp average fragment length
   5% of sequence in fragments shorter than    1058 bp
  10% of sequence in fragments shorter than    1616 bp
  15% of sequence in fragments shorter than    2139 bp
  20% of sequence in fragments shorter than    2637 bp
  25% of sequence in fragments shorter than    3096 bp
  30% of sequence in fragments shorter than    3583 bp
  35% of sequence in fragments shorter than    4084 bp
  40% of sequence in fragments shorter than    4598 bp
  45% of sequence in fragments shorter than    5130 bp
  50% of sequence in fragments shorter than    5700 bp
  55% of sequence in fragments shorter than    6316 bp
  60% of sequence in fragments shorter than    6996 bp
  65% of sequence in fragments shorter than    7771 bp
  70% of sequence in fragments shorter than    8667 bp
  75% of sequence in fragments shorter than    9739 bp
  80% of sequence in fragments shorter than   11075 bp
  85% of sequence in fragments shorter than   12863 bp
  90% of sequence in fragments shorter than   15623 bp
  95% of sequence in fragments shorter than   21516 bp

# ApaI.ApaLI (GGGCCC+GTGCAC) double digest, 956 thousand fragments (460339+496255 sites), 3116 bp average fragment length
   5% of sequence in fragments shorter than    1020 bp
  10% of sequence in fragments shorter than    1649 bp
  15% of sequence in fragments shorter than    2225 bp
  20% of sequence in fragments shorter than    2783 bp
  25% of sequence in fragments shorter than    3347 bp
  30% of sequence in fragments shorter than    3923 bp
  35% of sequence in fragments shorter than    4520 bp
  40% of sequence in fragments shorter than    5146 bp
  45% of sequence in fragments shorter than    5803 bp
  50% of sequence in fragments shorter than    6485 bp
  55% of sequence in fragments shorter than    7252 bp
  60% of sequence in fragments shorter than    8105 bp
  65% of sequence in fragments shorter than    9051 bp
  70% of sequence in fragments shorter than   10119 bp
  75% of sequence in fragments shorter than   11392 bp
  80% of sequence in fragments shorter than   12965 bp
  85% of sequence in fragments shorter than   15052 bp
  90% of sequence in fragments shorter than   18088 bp
  95% of sequence in fragments shorter than   24143 bp

# AvaIII.MluI (ATGCAT+ACGCGT) double digest, 954 thousand fragments (933357+21415 sites), 3122 bp average fragment length
   5% of sequence in fragments shorter than    1107 bp
  10% of sequence in fragments shorter than    1685 bp
  15% of sequence in fragments shorter than    2179 bp
  20% of sequence in fragments shorter than    2649 bp
  25% of sequence in fragments shorter than    3113 bp
  30% of sequence in fragments shorter than    3579 bp
  35% of sequence in fragments shorter than    4062 bp
  40% of sequence in fragments shorter than    4565 bp
  45% of sequence in fragments shorter than    5092 bp
  50% of sequence in fragments shorter than    5659 bp
  55% of sequence in fragments shorter than    6267 bp
  60% of sequence in fragments shorter than    6942 bp
  65% of sequence in fragments shorter than    7696 bp
  70% of sequence in fragments shorter than    8548 bp
  75% of sequence in fragments shorter than    9558 bp
  80% of sequence in fragments shorter than   10831 bp
  85% of sequence in fragments shorter than   12473 bp
  90% of sequence in fragments shorter than   14900 bp
  95% of sequence in fragments shorter than   19859 bp

# HpaI.MfeI (GTTAAC+CAATTG) double digest, 953 thousand fragments (389418+564303 sites), 3126 bp average fragment length
   5% of sequence in fragments shorter than    1078 bp
  10% of sequence in fragments shorter than    1650 bp
  15% of sequence in fragments shorter than    2105 bp
  20% of sequence in fragments shorter than    2564 bp
  25% of sequence in fragments shorter than    3008 bp
  30% of sequence in fragments shorter than    3452 bp
  35% of sequence in fragments shorter than    3912 bp
  40% of sequence in fragments shorter than    4387 bp
  45% of sequence in fragments shorter than    4890 bp
  50% of sequence in fragments shorter than    5432 bp
  55% of sequence in fragments shorter than    6012 bp
  60% of sequence in fragments shorter than    6655 bp
  65% of sequence in fragments shorter than    7374 bp
  70% of sequence in fragments shorter than    8213 bp
  75% of sequence in fragments shorter than    9204 bp
  80% of sequence in fragments shorter than   10451 bp
  85% of sequence in fragments shorter than   12135 bp
  90% of sequence in fragments shorter than   14710 bp
  95% of sequence in fragments shorter than   20098 bp

# SpeI.SphI (ACTAGT+GCATGC) double digest, 950 thousand fragments (400286+550952 sites), 3134 bp average fragment length
   5% of sequence in fragments shorter than    1110 bp
  10% of sequence in fragments shorter than    1664 bp
  15% of sequence in fragments shorter than    2133 bp
  20% of sequence in fragments shorter than    2585 bp
  25% of sequence in fragments shorter than    3022 bp
  30% of sequence in fragments shorter than    3460 bp
  35% of sequence in fragments shorter than    3897 bp
  40% of sequence in fragments shorter than    4352 bp
  45% of sequence in fragments shorter than    4827 bp
  50% of sequence in fragments shorter than    5322 bp
  55% of sequence in fragments shorter than    5855 bp
  60% of sequence in fragments shorter than    6436 bp
  65% of sequence in fragments shorter than    7077 bp
  70% of sequence in fragments shorter than    7807 bp
  75% of sequence in fragments shorter than    8651 bp
  80% of sequence in fragments shorter than    9693 bp
  85% of sequence in fragments shorter than   11024 bp
  90% of sequence in fragments shorter than   12927 bp
  95% of sequence in fragments shorter than   16534 bp

# BsaAI.BsrBI (YACGTR+CCGCTC) double digest, 948 thousand fragments (713801+235523 sites), 3140 bp average fragment length
   5% of sequence in fragments shorter than    1073 bp
  10% of sequence in fragments shorter than    1680 bp
  15% of sequence in fragments shorter than    2212 bp
  20% of sequence in fragments shorter than    2737 bp
  25% of sequence in fragments shorter than    3224 bp
  30% of sequence in fragments shorter than    3724 bp
  35% of sequence in fragments shorter than    4254 bp
  40% of sequence in fragments shorter than    4795 bp
  45% of sequence in fragments shorter than    5377 bp
  50% of sequence in fragments shorter than    5984 bp
  55% of sequence in fragments shorter than    6639 bp
  60% of sequence in fragments shorter than    7355 bp
  65% of sequence in fragments shorter than    8164 bp
  70% of sequence in fragments shorter than    9075 bp
  75% of sequence in fragments shorter than   10138 bp
  80% of sequence in fragments shorter than   11433 bp
  85% of sequence in fragments shorter than   13115 bp
  90% of sequence in fragments shorter than   15489 bp
  95% of sequence in fragments shorter than   19798 bp

# HgaI.SapI (GACGC+GCTCTTC) double digest, 948 thousand fragments (571889+377161 sites), 3141 bp average fragment length
   5% of sequence in fragments shorter than    1040 bp
  10% of sequence in fragments shorter than    1671 bp
  15% of sequence in fragments shorter than    2264 bp
  20% of sequence in fragments shorter than    2831 bp
  25% of sequence in fragments shorter than    3397 bp
  30% of sequence in fragments shorter than    3966 bp
  35% of sequence in fragments shorter than    4556 bp
  40% of sequence in fragments shorter than    5168 bp
  45% of sequence in fragments shorter than    5818 bp
  50% of sequence in fragments shorter than    6505 bp
  55% of sequence in fragments shorter than    7262 bp
  60% of sequence in fragments shorter than    8096 bp
  65% of sequence in fragments shorter than    9028 bp
  70% of sequence in fragments shorter than   10092 bp
  75% of sequence in fragments shorter than   11360 bp
  80% of sequence in fragments shorter than   12909 bp
  85% of sequence in fragments shorter than   14853 bp
  90% of sequence in fragments shorter than   17713 bp
  95% of sequence in fragments shorter than   23221 bp

# HgaI.SmaI (GACGC+CCCGGG) double digest, 948 thousand fragments (571889+376940 sites), 3142 bp average fragment length
   5% of sequence in fragments shorter than    1018 bp
  10% of sequence in fragments shorter than    1722 bp
  15% of sequence in fragments shorter than    2382 bp
  20% of sequence in fragments shorter than    3032 bp
  25% of sequence in fragments shorter than    3708 bp
  30% of sequence in fragments shorter than    4425 bp
  35% of sequence in fragments shorter than    5184 bp
  40% of sequence in fragments shorter than    5996 bp
  45% of sequence in fragments shorter than    6872 bp
  50% of sequence in fragments shorter than    7824 bp
  55% of sequence in fragments shorter than    8869 bp
  60% of sequence in fragments shorter than   10044 bp
  65% of sequence in fragments shorter than   11409 bp
  70% of sequence in fragments shorter than   12997 bp
  75% of sequence in fragments shorter than   14852 bp
  80% of sequence in fragments shorter than   17180 bp
  85% of sequence in fragments shorter than   20204 bp
  90% of sequence in fragments shorter than   24662 bp
  95% of sequence in fragments shorter than   33344 bp

# AvaIII.SgrAI (ATGCAT+CRCCGGYG) double digest, 948 thousand fragments (933357+15270 sites), 3142 bp average fragment length
   5% of sequence in fragments shorter than    1114 bp
  10% of sequence in fragments shorter than    1696 bp
  15% of sequence in fragments shorter than    2192 bp
  20% of sequence in fragments shorter than    2664 bp
  25% of sequence in fragments shorter than    3131 bp
  30% of sequence in fragments shorter than    3598 bp
  35% of sequence in fragments shorter than    4085 bp
  40% of sequence in fragments shorter than    4591 bp
  45% of sequence in fragments shorter than    5119 bp
  50% of sequence in fragments shorter than    5687 bp
  55% of sequence in fragments shorter than    6300 bp
  60% of sequence in fragments shorter than    6982 bp
  65% of sequence in fragments shorter than    7744 bp
  70% of sequence in fragments shorter than    8601 bp
  75% of sequence in fragments shorter than    9617 bp
  80% of sequence in fragments shorter than   10904 bp
  85% of sequence in fragments shorter than   12557 bp
  90% of sequence in fragments shorter than   15005 bp
  95% of sequence in fragments shorter than   19982 bp

# EciI.SapI (GGCGGA+GCTCTTC) double digest, 948 thousand fragments (571273+377161 sites), 3143 bp average fragment length
   5% of sequence in fragments shorter than    1040 bp
  10% of sequence in fragments shorter than    1645 bp
  15% of sequence in fragments shorter than    2193 bp
  20% of sequence in fragments shorter than    2719 bp
  25% of sequence in fragments shorter than    3241 bp
  30% of sequence in fragments shorter than    3788 bp
  35% of sequence in fragments shorter than    4345 bp
  40% of sequence in fragments shorter than    4931 bp
  45% of sequence in fragments shorter than    5553 bp
  50% of sequence in fragments shorter than    6225 bp
  55% of sequence in fragments shorter than    6971 bp
  60% of sequence in fragments shorter than    7789 bp
  65% of sequence in fragments shorter than    8703 bp
  70% of sequence in fragments shorter than    9763 bp
  75% of sequence in fragments shorter than   11038 bp
  80% of sequence in fragments shorter than   12630 bp
  85% of sequence in fragments shorter than   14755 bp
  90% of sequence in fragments shorter than   17831 bp
  95% of sequence in fragments shorter than   24217 bp

# EciI.SmaI (GGCGGA+CCCGGG) double digest, 947 thousand fragments (571273+376940 sites), 3143 bp average fragment length
   5% of sequence in fragments shorter than    1045 bp
  10% of sequence in fragments shorter than    1772 bp
  15% of sequence in fragments shorter than    2467 bp
  20% of sequence in fragments shorter than    3181 bp
  25% of sequence in fragments shorter than    3939 bp
  30% of sequence in fragments shorter than    4764 bp
  35% of sequence in fragments shorter than    5669 bp
  40% of sequence in fragments shorter than    6663 bp
  45% of sequence in fragments shorter than    7741 bp
  50% of sequence in fragments shorter than    8957 bp
  55% of sequence in fragments shorter than   10318 bp
  60% of sequence in fragments shorter than   11858 bp
  65% of sequence in fragments shorter than   13651 bp
  70% of sequence in fragments shorter than   15743 bp
  75% of sequence in fragments shorter than   18269 bp
  80% of sequence in fragments shorter than   21413 bp
  85% of sequence in fragments shorter than   25812 bp
  90% of sequence in fragments shorter than   32182 bp
  95% of sequence in fragments shorter than   45373 bp

# AvaIII.NruI (ATGCAT+TCGCGA) double digest, 947 thousand fragments (933357+14872 sites), 3144 bp average fragment length
   5% of sequence in fragments shorter than    1113 bp
  10% of sequence in fragments shorter than    1695 bp
  15% of sequence in fragments shorter than    2191 bp
  20% of sequence in fragments shorter than    2664 bp
  25% of sequence in fragments shorter than    3131 bp
  30% of sequence in fragments shorter than    3600 bp
  35% of sequence in fragments shorter than    4088 bp
  40% of sequence in fragments shorter than    4593 bp
  45% of sequence in fragments shorter than    5123 bp
  50% of sequence in fragments shorter than    5693 bp
  55% of sequence in fragments shorter than    6306 bp
  60% of sequence in fragments shorter than    6989 bp
  65% of sequence in fragments shorter than    7753 bp
  70% of sequence in fragments shorter than    8612 bp
  75% of sequence in fragments shorter than    9636 bp
  80% of sequence in fragments shorter than   10930 bp
  85% of sequence in fragments shorter than   12593 bp
  90% of sequence in fragments shorter than   15066 bp
  95% of sequence in fragments shorter than   20146 bp

# AvaIII.PvuI (ATGCAT+CGATCG) double digest, 947 thousand fragments (933357+14186 sites), 3146 bp average fragment length
   5% of sequence in fragments shorter than    1115 bp
  10% of sequence in fragments shorter than    1698 bp
  15% of sequence in fragments shorter than    2189 bp
  20% of sequence in fragments shorter than    2664 bp
  25% of sequence in fragments shorter than    3134 bp
  30% of sequence in fragments shorter than    3604 bp
  35% of sequence in fragments shorter than    4090 bp
  40% of sequence in fragments shorter than    4597 bp
  45% of sequence in fragments shorter than    5128 bp
  50% of sequence in fragments shorter than    5698 bp
  55% of sequence in fragments shorter than    6314 bp
  60% of sequence in fragments shorter than    7000 bp
  65% of sequence in fragments shorter than    7764 bp
  70% of sequence in fragments shorter than    8629 bp
  75% of sequence in fragments shorter than    9657 bp
  80% of sequence in fragments shorter than   10948 bp
  85% of sequence in fragments shorter than   12622 bp
  90% of sequence in fragments shorter than   15098 bp
  95% of sequence in fragments shorter than   20141 bp

# AclI.BglII (AACGTT+AGATCT) double digest, 947 thousand fragments (172706+774732 sites), 3146 bp average fragment length
   5% of sequence in fragments shorter than    1087 bp
  10% of sequence in fragments shorter than    1645 bp
  15% of sequence in fragments shorter than    2113 bp
  20% of sequence in fragments shorter than    2573 bp
  25% of sequence in fragments shorter than    3006 bp
  30% of sequence in fragments shorter than    3450 bp
  35% of sequence in fragments shorter than    3901 bp
  40% of sequence in fragments shorter than    4362 bp
  45% of sequence in fragments shorter than    4848 bp
  50% of sequence in fragments shorter than    5358 bp
  55% of sequence in fragments shorter than    5914 bp
  60% of sequence in fragments shorter than    6518 bp
  65% of sequence in fragments shorter than    7196 bp
  70% of sequence in fragments shorter than    7961 bp
  75% of sequence in fragments shorter than    8843 bp
  80% of sequence in fragments shorter than    9940 bp
  85% of sequence in fragments shorter than   11337 bp
  90% of sequence in fragments shorter than   13409 bp
  95% of sequence in fragments shorter than   17196 bp

# MluI.StuI (ACGCGT+AGGCCT) double digest, 946 thousand fragments (21415+925729 sites), 3147 bp average fragment length
   5% of sequence in fragments shorter than     938 bp
  10% of sequence in fragments shorter than    1607 bp
  15% of sequence in fragments shorter than    2209 bp
  20% of sequence in fragments shorter than    2819 bp
  25% of sequence in fragments shorter than    3416 bp
  30% of sequence in fragments shorter than    4011 bp
  35% of sequence in fragments shorter than    4622 bp
  40% of sequence in fragments shorter than    5265 bp
  45% of sequence in fragments shorter than    5934 bp
  50% of sequence in fragments shorter than    6655 bp
  55% of sequence in fragments shorter than    7432 bp
  60% of sequence in fragments shorter than    8266 bp
  65% of sequence in fragments shorter than    9197 bp
  70% of sequence in fragments shorter than   10258 bp
  75% of sequence in fragments shorter than   11484 bp
  80% of sequence in fragments shorter than   12955 bp
  85% of sequence in fragments shorter than   14857 bp
  90% of sequence in fragments shorter than   17463 bp
  95% of sequence in fragments shorter than   22010 bp

# AvaIII.FseI (ATGCAT+GGCCGGCC) double digest, 946 thousand fragments (933357+13662 sites), 3148 bp average fragment length
   5% of sequence in fragments shorter than    1116 bp
  10% of sequence in fragments shorter than    1700 bp
  15% of sequence in fragments shorter than    2197 bp
  20% of sequence in fragments shorter than    2671 bp
  25% of sequence in fragments shorter than    3139 bp
  30% of sequence in fragments shorter than    3608 bp
  35% of sequence in fragments shorter than    4095 bp
  40% of sequence in fragments shorter than    4600 bp
  45% of sequence in fragments shorter than    5129 bp
  50% of sequence in fragments shorter than    5698 bp
  55% of sequence in fragments shorter than    6311 bp
  60% of sequence in fragments shorter than    6992 bp
  65% of sequence in fragments shorter than    7751 bp
  70% of sequence in fragments shorter than    8604 bp
  75% of sequence in fragments shorter than    9616 bp
  80% of sequence in fragments shorter than   10897 bp
  85% of sequence in fragments shorter than   12539 bp
  90% of sequence in fragments shorter than   14959 bp
  95% of sequence in fragments shorter than   19860 bp

# ClaI.HindIII (ATCGAT+AAGCTT) double digest, 946 thousand fragments (86477+860365 sites), 3148 bp average fragment length
   5% of sequence in fragments shorter than    1106 bp
  10% of sequence in fragments shorter than    1681 bp
  15% of sequence in fragments shorter than    2137 bp
  20% of sequence in fragments shorter than    2605 bp
  25% of sequence in fragments shorter than    3052 bp
  30% of sequence in fragments shorter than    3509 bp
  35% of sequence in fragments shorter than    3972 bp
  40% of sequence in fragments shorter than    4446 bp
  45% of sequence in fragments shorter than    4941 bp
  50% of sequence in fragments shorter than    5471 bp
  55% of sequence in fragments shorter than    6045 bp
  60% of sequence in fragments shorter than    6670 bp
  65% of sequence in fragments shorter than    7369 bp
  70% of sequence in fragments shorter than    8167 bp
  75% of sequence in fragments shorter than    9095 bp
  80% of sequence in fragments shorter than   10232 bp
  85% of sequence in fragments shorter than   11725 bp
  90% of sequence in fragments shorter than   13893 bp
  95% of sequence in fragments shorter than   18111 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# NdeI.PmeI (CATATG+GTTTAAAC) double digest, 945 thousand fragments (903360+42094 sites), 3153 bp average fragment length
   5% of sequence in fragments shorter than    1059 bp
  10% of sequence in fragments shorter than    1622 bp
  15% of sequence in fragments shorter than    2156 bp
  20% of sequence in fragments shorter than    2664 bp
  25% of sequence in fragments shorter than    3156 bp
  30% of sequence in fragments shorter than    3658 bp
  35% of sequence in fragments shorter than    4171 bp
  40% of sequence in fragments shorter than    4701 bp
  45% of sequence in fragments shorter than    5243 bp
  50% of sequence in fragments shorter than    5833 bp
  55% of sequence in fragments shorter than    6472 bp
  60% of sequence in fragments shorter than    7187 bp
  65% of sequence in fragments shorter than    8000 bp
  70% of sequence in fragments shorter than    8950 bp
  75% of sequence in fragments shorter than   10095 bp
  80% of sequence in fragments shorter than   11534 bp
  85% of sequence in fragments shorter than   13559 bp
  90% of sequence in fragments shorter than   16759 bp
  95% of sequence in fragments shorter than   24176 bp

# BsaAI.KasI (YACGTR+GGCGCC) double digest, 943 thousand fragments (713801+230381 sites), 3157 bp average fragment length
   5% of sequence in fragments shorter than    1077 bp
  10% of sequence in fragments shorter than    1688 bp
  15% of sequence in fragments shorter than    2203 bp
  20% of sequence in fragments shorter than    2711 bp
  25% of sequence in fragments shorter than    3196 bp
  30% of sequence in fragments shorter than    3670 bp
  35% of sequence in fragments shorter than    4181 bp
  40% of sequence in fragments shorter than    4696 bp
  45% of sequence in fragments shorter than    5256 bp
  50% of sequence in fragments shorter than    5849 bp
  55% of sequence in fragments shorter than    6490 bp
  60% of sequence in fragments shorter than    7185 bp
  65% of sequence in fragments shorter than    7977 bp
  70% of sequence in fragments shorter than    8877 bp
  75% of sequence in fragments shorter than    9921 bp
  80% of sequence in fragments shorter than   11205 bp
  85% of sequence in fragments shorter than   12863 bp
  90% of sequence in fragments shorter than   15222 bp
  95% of sequence in fragments shorter than   19573 bp

# ScaI.SpeI (AGTACT+ACTAGT) double digest, 943 thousand fragments (543793+400286 sites), 3157 bp average fragment length
   5% of sequence in fragments shorter than    1104 bp
  10% of sequence in fragments shorter than    1664 bp
  15% of sequence in fragments shorter than    2146 bp
  20% of sequence in fragments shorter than    2607 bp
  25% of sequence in fragments shorter than    3065 bp
  30% of sequence in fragments shorter than    3529 bp
  35% of sequence in fragments shorter than    4003 bp
  40% of sequence in fragments shorter than    4500 bp
  45% of sequence in fragments shorter than    5022 bp
  50% of sequence in fragments shorter than    5589 bp
  55% of sequence in fragments shorter than    6188 bp
  60% of sequence in fragments shorter than    6847 bp
  65% of sequence in fragments shorter than    7598 bp
  70% of sequence in fragments shorter than    8460 bp
  75% of sequence in fragments shorter than    9501 bp
  80% of sequence in fragments shorter than   10804 bp
  85% of sequence in fragments shorter than   12558 bp
  90% of sequence in fragments shorter than   15287 bp
  95% of sequence in fragments shorter than   21320 bp

# AvaIII.NotI (ATGCAT+GCGGCCGC) double digest, 942 thousand fragments (933357+9795 sites), 3160 bp average fragment length
   5% of sequence in fragments shorter than    1120 bp
  10% of sequence in fragments shorter than    1707 bp
  15% of sequence in fragments shorter than    2206 bp
  20% of sequence in fragments shorter than    2680 bp
  25% of sequence in fragments shorter than    3151 bp
  30% of sequence in fragments shorter than    3624 bp
  35% of sequence in fragments shorter than    4115 bp
  40% of sequence in fragments shorter than    4623 bp
  45% of sequence in fragments shorter than    5158 bp
  50% of sequence in fragments shorter than    5732 bp
  55% of sequence in fragments shorter than    6352 bp
  60% of sequence in fragments shorter than    7040 bp
  65% of sequence in fragments shorter than    7806 bp
  70% of sequence in fragments shorter than    8671 bp
  75% of sequence in fragments shorter than    9702 bp
  80% of sequence in fragments shorter than   10999 bp
  85% of sequence in fragments shorter than   12680 bp
  90% of sequence in fragments shorter than   15169 bp
  95% of sequence in fragments shorter than   20236 bp

# AvaIII.RsrII (ATGCAT+CGGWCCG) double digest, 942 thousand fragments (933357+9764 sites), 3161 bp average fragment length
   5% of sequence in fragments shorter than    1119 bp
  10% of sequence in fragments shorter than    1706 bp
  15% of sequence in fragments shorter than    2204 bp
  20% of sequence in fragments shorter than    2678 bp
  25% of sequence in fragments shorter than    3150 bp
  30% of sequence in fragments shorter than    3622 bp
  35% of sequence in fragments shorter than    4113 bp
  40% of sequence in fragments shorter than    4621 bp
  45% of sequence in fragments shorter than    5155 bp
  50% of sequence in fragments shorter than    5727 bp
  55% of sequence in fragments shorter than    6347 bp
  60% of sequence in fragments shorter than    7037 bp
  65% of sequence in fragments shorter than    7802 bp
  70% of sequence in fragments shorter than    8672 bp
  75% of sequence in fragments shorter than    9700 bp
  80% of sequence in fragments shorter than   11001 bp
  85% of sequence in fragments shorter than   12672 bp
  90% of sequence in fragments shorter than   15171 bp
  95% of sequence in fragments shorter than   20294 bp

# ApaLI.EcoRV (GTGCAC+GATATC) double digest, 942 thousand fragments (496255+446473 sites), 3162 bp average fragment length
   5% of sequence in fragments shorter than    1114 bp
  10% of sequence in fragments shorter than    1699 bp
  15% of sequence in fragments shorter than    2182 bp
  20% of sequence in fragments shorter than    2642 bp
  25% of sequence in fragments shorter than    3049 bp
  30% of sequence in fragments shorter than    3491 bp
  35% of sequence in fragments shorter than    3950 bp
  40% of sequence in fragments shorter than    4416 bp
  45% of sequence in fragments shorter than    4906 bp
  50% of sequence in fragments shorter than    5424 bp
  55% of sequence in fragments shorter than    5978 bp
  60% of sequence in fragments shorter than    6575 bp
  65% of sequence in fragments shorter than    7238 bp
  70% of sequence in fragments shorter than    7994 bp
  75% of sequence in fragments shorter than    8861 bp
  80% of sequence in fragments shorter than    9898 bp
  85% of sequence in fragments shorter than   11263 bp
  90% of sequence in fragments shorter than   13161 bp
  95% of sequence in fragments shorter than   16678 bp

# MfeI.SapI (CAATTG+GCTCTTC) double digest, 941 thousand fragments (564303+377161 sites), 3166 bp average fragment length
   5% of sequence in fragments shorter than    1096 bp
  10% of sequence in fragments shorter than    1676 bp
  15% of sequence in fragments shorter than    2124 bp
  20% of sequence in fragments shorter than    2580 bp
  25% of sequence in fragments shorter than    3018 bp
  30% of sequence in fragments shorter than    3464 bp
  35% of sequence in fragments shorter than    3924 bp
  40% of sequence in fragments shorter than    4391 bp
  45% of sequence in fragments shorter than    4880 bp
  50% of sequence in fragments shorter than    5403 bp
  55% of sequence in fragments shorter than    5966 bp
  60% of sequence in fragments shorter than    6579 bp
  65% of sequence in fragments shorter than    7254 bp
  70% of sequence in fragments shorter than    8032 bp
  75% of sequence in fragments shorter than    8945 bp
  80% of sequence in fragments shorter than   10057 bp
  85% of sequence in fragments shorter than   11498 bp
  90% of sequence in fragments shorter than   13566 bp
  95% of sequence in fragments shorter than   17570 bp

# AflII.SexAI (CTTAAG+ACCWGGT) double digest, 940 thousand fragments (643284+297900 sites), 3167 bp average fragment length
   5% of sequence in fragments shorter than    1117 bp
  10% of sequence in fragments shorter than    1687 bp
  15% of sequence in fragments shorter than    2169 bp
  20% of sequence in fragments shorter than    2621 bp
  25% of sequence in fragments shorter than    3061 bp
  30% of sequence in fragments shorter than    3508 bp
  35% of sequence in fragments shorter than    3946 bp
  40% of sequence in fragments shorter than    4389 bp
  45% of sequence in fragments shorter than    4866 bp
  50% of sequence in fragments shorter than    5374 bp
  55% of sequence in fragments shorter than    5916 bp
  60% of sequence in fragments shorter than    6515 bp
  65% of sequence in fragments shorter than    7168 bp
  70% of sequence in fragments shorter than    7913 bp
  75% of sequence in fragments shorter than    8788 bp
  80% of sequence in fragments shorter than    9846 bp
  85% of sequence in fragments shorter than   11233 bp
  90% of sequence in fragments shorter than   13270 bp
  95% of sequence in fragments shorter than   17428 bp

# MfeI.SmaI (CAATTG+CCCGGG) double digest, 940 thousand fragments (564303+376940 sites), 3167 bp average fragment length
   5% of sequence in fragments shorter than    1087 bp
  10% of sequence in fragments shorter than    1681 bp
  15% of sequence in fragments shorter than    2175 bp
  20% of sequence in fragments shorter than    2652 bp
  25% of sequence in fragments shorter than    3114 bp
  30% of sequence in fragments shorter than    3581 bp
  35% of sequence in fragments shorter than    4055 bp
  40% of sequence in fragments shorter than    4531 bp
  45% of sequence in fragments shorter than    5035 bp
  50% of sequence in fragments shorter than    5567 bp
  55% of sequence in fragments shorter than    6150 bp
  60% of sequence in fragments shorter than    6772 bp
  65% of sequence in fragments shorter than    7467 bp
  70% of sequence in fragments shorter than    8271 bp
  75% of sequence in fragments shorter than    9186 bp
  80% of sequence in fragments shorter than   10321 bp
  85% of sequence in fragments shorter than   11741 bp
  90% of sequence in fragments shorter than   13805 bp
  95% of sequence in fragments shorter than   17686 bp

# SgrAI.StuI (CRCCGGYG+AGGCCT) double digest, 940 thousand fragments (15270+925729 sites), 3168 bp average fragment length
   5% of sequence in fragments shorter than     945 bp
  10% of sequence in fragments shorter than    1619 bp
  15% of sequence in fragments shorter than    2227 bp
  20% of sequence in fragments shorter than    2845 bp
  25% of sequence in fragments shorter than    3444 bp
  30% of sequence in fragments shorter than    4048 bp
  35% of sequence in fragments shorter than    4666 bp
  40% of sequence in fragments shorter than    5316 bp
  45% of sequence in fragments shorter than    5989 bp
  50% of sequence in fragments shorter than    6720 bp
  55% of sequence in fragments shorter than    7502 bp
  60% of sequence in fragments shorter than    8341 bp
  65% of sequence in fragments shorter than    9287 bp
  70% of sequence in fragments shorter than   10356 bp
  75% of sequence in fragments shorter than   11604 bp
  80% of sequence in fragments shorter than   13086 bp
  85% of sequence in fragments shorter than   14997 bp
  90% of sequence in fragments shorter than   17636 bp
  95% of sequence in fragments shorter than   22271 bp

# BceAI.BsrBI (ACGGC+CCGCTC) double digest, 940 thousand fragments (705178+235523 sites), 3169 bp average fragment length
   5% of sequence in fragments shorter than    1034 bp
  10% of sequence in fragments shorter than    1737 bp
  15% of sequence in fragments shorter than    2384 bp
  20% of sequence in fragments shorter than    3033 bp
  25% of sequence in fragments shorter than    3695 bp
  30% of sequence in fragments shorter than    4376 bp
  35% of sequence in fragments shorter than    5088 bp
  40% of sequence in fragments shorter than    5841 bp
  45% of sequence in fragments shorter than    6640 bp
  50% of sequence in fragments shorter than    7509 bp
  55% of sequence in fragments shorter than    8455 bp
  60% of sequence in fragments shorter than    9506 bp
  65% of sequence in fragments shorter than   10694 bp
  70% of sequence in fragments shorter than   12069 bp
  75% of sequence in fragments shorter than   13679 bp
  80% of sequence in fragments shorter than   15681 bp
  85% of sequence in fragments shorter than   18223 bp
  90% of sequence in fragments shorter than   21948 bp
  95% of sequence in fragments shorter than   28780 bp

# NruI.StuI (TCGCGA+AGGCCT) double digest, 940 thousand fragments (14872+925729 sites), 3169 bp average fragment length
   5% of sequence in fragments shorter than     947 bp
  10% of sequence in fragments shorter than    1621 bp
  15% of sequence in fragments shorter than    2228 bp
  20% of sequence in fragments shorter than    2843 bp
  25% of sequence in fragments shorter than    3439 bp
  30% of sequence in fragments shorter than    4038 bp
  35% of sequence in fragments shorter than    4652 bp
  40% of sequence in fragments shorter than    5298 bp
  45% of sequence in fragments shorter than    5968 bp
  50% of sequence in fragments shorter than    6695 bp
  55% of sequence in fragments shorter than    7473 bp
  60% of sequence in fragments shorter than    8308 bp
  65% of sequence in fragments shorter than    9245 bp
  70% of sequence in fragments shorter than   10312 bp
  75% of sequence in fragments shorter than   11549 bp
  80% of sequence in fragments shorter than   13028 bp
  85% of sequence in fragments shorter than   14924 bp
  90% of sequence in fragments shorter than   17552 bp
  95% of sequence in fragments shorter than   22086 bp

# HpaI.SphI (GTTAAC+GCATGC) double digest, 939 thousand fragments (389418+550952 sites), 3170 bp average fragment length
   5% of sequence in fragments shorter than    1116 bp
  10% of sequence in fragments shorter than    1678 bp
  15% of sequence in fragments shorter than    2161 bp
  20% of sequence in fragments shorter than    2622 bp
  25% of sequence in fragments shorter than    3072 bp
  30% of sequence in fragments shorter than    3519 bp
  35% of sequence in fragments shorter than    3971 bp
  40% of sequence in fragments shorter than    4436 bp
  45% of sequence in fragments shorter than    4928 bp
  50% of sequence in fragments shorter than    5449 bp
  55% of sequence in fragments shorter than    6001 bp
  60% of sequence in fragments shorter than    6606 bp
  65% of sequence in fragments shorter than    7284 bp
  70% of sequence in fragments shorter than    8051 bp
  75% of sequence in fragments shorter than    8939 bp
  80% of sequence in fragments shorter than   10008 bp
  85% of sequence in fragments shorter than   11414 bp
  90% of sequence in fragments shorter than   13479 bp
  95% of sequence in fragments shorter than   17564 bp

# BsaAI.SwaI (YACGTR+ATTTAAAT) double digest, 939 thousand fragments (713801+226602 sites), 3170 bp average fragment length
   5% of sequence in fragments shorter than    1121 bp
  10% of sequence in fragments shorter than    1707 bp
  15% of sequence in fragments shorter than    2203 bp
  20% of sequence in fragments shorter than    2682 bp
  25% of sequence in fragments shorter than    3136 bp
  30% of sequence in fragments shorter than    3578 bp
  35% of sequence in fragments shorter than    4051 bp
  40% of sequence in fragments shorter than    4526 bp
  45% of sequence in fragments shorter than    5036 bp
  50% of sequence in fragments shorter than    5575 bp
  55% of sequence in fragments shorter than    6152 bp
  60% of sequence in fragments shorter than    6787 bp
  65% of sequence in fragments shorter than    7478 bp
  70% of sequence in fragments shorter than    8258 bp
  75% of sequence in fragments shorter than    9167 bp
  80% of sequence in fragments shorter than   10254 bp
  85% of sequence in fragments shorter than   11667 bp
  90% of sequence in fragments shorter than   13703 bp
  95% of sequence in fragments shorter than   17446 bp

# PvuI.StuI (CGATCG+AGGCCT) double digest, 939 thousand fragments (14186+925729 sites), 3172 bp average fragment length
   5% of sequence in fragments shorter than     950 bp
  10% of sequence in fragments shorter than    1623 bp
  15% of sequence in fragments shorter than    2224 bp
  20% of sequence in fragments shorter than    2839 bp
  25% of sequence in fragments shorter than    3438 bp
  30% of sequence in fragments shorter than    4039 bp
  35% of sequence in fragments shorter than    4656 bp
  40% of sequence in fragments shorter than    5305 bp
  45% of sequence in fragments shorter than    5977 bp
  50% of sequence in fragments shorter than    6701 bp
  55% of sequence in fragments shorter than    7483 bp
  60% of sequence in fragments shorter than    8317 bp
  65% of sequence in fragments shorter than    9260 bp
  70% of sequence in fragments shorter than   10324 bp
  75% of sequence in fragments shorter than   11560 bp
  80% of sequence in fragments shorter than   13037 bp
  85% of sequence in fragments shorter than   14947 bp
  90% of sequence in fragments shorter than   17554 bp
  95% of sequence in fragments shorter than   22112 bp

# FseI.StuI (GGCCGGCC+AGGCCT) double digest, 938 thousand fragments (13662+925729 sites), 3173 bp average fragment length
   5% of sequence in fragments shorter than     947 bp
  10% of sequence in fragments shorter than    1622 bp
  15% of sequence in fragments shorter than    2232 bp
  20% of sequence in fragments shorter than    2852 bp
  25% of sequence in fragments shorter than    3451 bp
  30% of sequence in fragments shorter than    4058 bp
  35% of sequence in fragments shorter than    4678 bp
  40% of sequence in fragments shorter than    5329 bp
  45% of sequence in fragments shorter than    6003 bp
  50% of sequence in fragments shorter than    6736 bp
  55% of sequence in fragments shorter than    7525 bp
  60% of sequence in fragments shorter than    8366 bp
  65% of sequence in fragments shorter than    9312 bp
  70% of sequence in fragments shorter than   10393 bp
  75% of sequence in fragments shorter than   11642 bp
  80% of sequence in fragments shorter than   13129 bp
  85% of sequence in fragments shorter than   15048 bp
  90% of sequence in fragments shorter than   17693 bp
  95% of sequence in fragments shorter than   22364 bp

# BstEII.SacI (GGTNACC+GAGCTC) double digest, 938 thousand fragments (335865+603323 sites), 3174 bp average fragment length
   5% of sequence in fragments shorter than    1070 bp
  10% of sequence in fragments shorter than    1665 bp
  15% of sequence in fragments shorter than    2183 bp
  20% of sequence in fragments shorter than    2682 bp
  25% of sequence in fragments shorter than    3181 bp
  30% of sequence in fragments shorter than    3681 bp
  35% of sequence in fragments shorter than    4200 bp
  40% of sequence in fragments shorter than    4733 bp
  45% of sequence in fragments shorter than    5285 bp
  50% of sequence in fragments shorter than    5885 bp
  55% of sequence in fragments shorter than    6538 bp
  60% of sequence in fragments shorter than    7244 bp
  65% of sequence in fragments shorter than    8041 bp
  70% of sequence in fragments shorter than    8953 bp
  75% of sequence in fragments shorter than   10041 bp
  80% of sequence in fragments shorter than   11371 bp
  85% of sequence in fragments shorter than   13102 bp
  90% of sequence in fragments shorter than   15618 bp
  95% of sequence in fragments shorter than   20630 bp

# AscI.AvaIII (GGCGCGCC+ATGCAT) double digest, 937 thousand fragments (4751+933357 sites), 3177 bp average fragment length
   5% of sequence in fragments shorter than    1124 bp
  10% of sequence in fragments shorter than    1714 bp
  15% of sequence in fragments shorter than    2216 bp
  20% of sequence in fragments shorter than    2693 bp
  25% of sequence in fragments shorter than    3167 bp
  30% of sequence in fragments shorter than    3642 bp
  35% of sequence in fragments shorter than    4136 bp
  40% of sequence in fragments shorter than    4649 bp
  45% of sequence in fragments shorter than    5190 bp
  50% of sequence in fragments shorter than    5768 bp
  55% of sequence in fragments shorter than    6392 bp
  60% of sequence in fragments shorter than    7089 bp
  65% of sequence in fragments shorter than    7863 bp
  70% of sequence in fragments shorter than    8750 bp
  75% of sequence in fragments shorter than    9797 bp
  80% of sequence in fragments shorter than   11118 bp
  85% of sequence in fragments shorter than   12842 bp
  90% of sequence in fragments shorter than   15397 bp
  95% of sequence in fragments shorter than   20768 bp

# BamHI.HgaI (GGATCC+GACGC) double digest, 937 thousand fragments (365999+571889 sites), 3178 bp average fragment length
   5% of sequence in fragments shorter than    1066 bp
  10% of sequence in fragments shorter than    1712 bp
  15% of sequence in fragments shorter than    2289 bp
  20% of sequence in fragments shorter than    2860 bp
  25% of sequence in fragments shorter than    3430 bp
  30% of sequence in fragments shorter than    4016 bp
  35% of sequence in fragments shorter than    4622 bp
  40% of sequence in fragments shorter than    5253 bp
  45% of sequence in fragments shorter than    5930 bp
  50% of sequence in fragments shorter than    6651 bp
  55% of sequence in fragments shorter than    7446 bp
  60% of sequence in fragments shorter than    8324 bp
  65% of sequence in fragments shorter than    9308 bp
  70% of sequence in fragments shorter than   10432 bp
  75% of sequence in fragments shorter than   11745 bp
  80% of sequence in fragments shorter than   13376 bp
  85% of sequence in fragments shorter than   15511 bp
  90% of sequence in fragments shorter than   18518 bp
  95% of sequence in fragments shorter than   24337 bp

# ClaI.XbaI (ATCGAT+TCTAGA) double digest, 937 thousand fragments (86477+850998 sites), 3180 bp average fragment length
   5% of sequence in fragments shorter than    1072 bp
  10% of sequence in fragments shorter than    1642 bp
  15% of sequence in fragments shorter than    2141 bp
  20% of sequence in fragments shorter than    2617 bp
  25% of sequence in fragments shorter than    3097 bp
  30% of sequence in fragments shorter than    3572 bp
  35% of sequence in fragments shorter than    4055 bp
  40% of sequence in fragments shorter than    4552 bp
  45% of sequence in fragments shorter than    5073 bp
  50% of sequence in fragments shorter than    5620 bp
  55% of sequence in fragments shorter than    6212 bp
  60% of sequence in fragments shorter than    6861 bp
  65% of sequence in fragments shorter than    7584 bp
  70% of sequence in fragments shorter than    8402 bp
  75% of sequence in fragments shorter than    9355 bp
  80% of sequence in fragments shorter than   10517 bp
  85% of sequence in fragments shorter than   12040 bp
  90% of sequence in fragments shorter than   14204 bp
  95% of sequence in fragments shorter than   18122 bp

# BamHI.EciI (GGATCC+GGCGGA) double digest, 936 thousand fragments (365999+571273 sites), 3180 bp average fragment length
   5% of sequence in fragments shorter than    1059 bp
  10% of sequence in fragments shorter than    1675 bp
  15% of sequence in fragments shorter than    2222 bp
  20% of sequence in fragments shorter than    2768 bp
  25% of sequence in fragments shorter than    3309 bp
  30% of sequence in fragments shorter than    3877 bp
  35% of sequence in fragments shorter than    4460 bp
  40% of sequence in fragments shorter than    5077 bp
  45% of sequence in fragments shorter than    5737 bp
  50% of sequence in fragments shorter than    6450 bp
  55% of sequence in fragments shorter than    7225 bp
  60% of sequence in fragments shorter than    8102 bp
  65% of sequence in fragments shorter than    9092 bp
  70% of sequence in fragments shorter than   10257 bp
  75% of sequence in fragments shorter than   11621 bp
  80% of sequence in fragments shorter than   13297 bp
  85% of sequence in fragments shorter than   15552 bp
  90% of sequence in fragments shorter than   18901 bp
  95% of sequence in fragments shorter than   25630 bp

# BceAI.KasI (ACGGC+GGCGCC) double digest, 935 thousand fragments (705178+230381 sites), 3186 bp average fragment length
   5% of sequence in fragments shorter than    1049 bp
  10% of sequence in fragments shorter than    1744 bp
  15% of sequence in fragments shorter than    2389 bp
  20% of sequence in fragments shorter than    3021 bp
  25% of sequence in fragments shorter than    3663 bp
  30% of sequence in fragments shorter than    4327 bp
  35% of sequence in fragments shorter than    5024 bp
  40% of sequence in fragments shorter than    5762 bp
  45% of sequence in fragments shorter than    6539 bp
  50% of sequence in fragments shorter than    7383 bp
  55% of sequence in fragments shorter than    8325 bp
  60% of sequence in fragments shorter than    9355 bp
  65% of sequence in fragments shorter than   10527 bp
  70% of sequence in fragments shorter than   11889 bp
  75% of sequence in fragments shorter than   13491 bp
  80% of sequence in fragments shorter than   15470 bp
  85% of sequence in fragments shorter than   18065 bp
  90% of sequence in fragments shorter than   21864 bp
  95% of sequence in fragments shorter than   29058 bp

# NotI.StuI (GCGGCCGC+AGGCCT) double digest, 935 thousand fragments (9795+925729 sites), 3186 bp average fragment length
   5% of sequence in fragments shorter than     951 bp
  10% of sequence in fragments shorter than    1631 bp
  15% of sequence in fragments shorter than    2243 bp
  20% of sequence in fragments shorter than    2866 bp
  25% of sequence in fragments shorter than    3469 bp
  30% of sequence in fragments shorter than    4077 bp
  35% of sequence in fragments shorter than    4700 bp
  40% of sequence in fragments shorter than    5356 bp
  45% of sequence in fragments shorter than    6033 bp
  50% of sequence in fragments shorter than    6767 bp
  55% of sequence in fragments shorter than    7556 bp
  60% of sequence in fragments shorter than    8399 bp
  65% of sequence in fragments shorter than    9349 bp
  70% of sequence in fragments shorter than   10426 bp
  75% of sequence in fragments shorter than   11675 bp
  80% of sequence in fragments shorter than   13171 bp
  85% of sequence in fragments shorter than   15087 bp
  90% of sequence in fragments shorter than   17734 bp
  95% of sequence in fragments shorter than   22383 bp

# RsrII.StuI (CGGWCCG+AGGCCT) double digest, 935 thousand fragments (9764+925729 sites), 3187 bp average fragment length
   5% of sequence in fragments shorter than     952 bp
  10% of sequence in fragments shorter than    1631 bp
  15% of sequence in fragments shorter than    2242 bp
  20% of sequence in fragments shorter than    2863 bp
  25% of sequence in fragments shorter than    3464 bp
  30% of sequence in fragments shorter than    4072 bp
  35% of sequence in fragments shorter than    4694 bp
  40% of sequence in fragments shorter than    5347 bp
  45% of sequence in fragments shorter than    6021 bp
  50% of sequence in fragments shorter than    6755 bp
  55% of sequence in fragments shorter than    7543 bp
  60% of sequence in fragments shorter than    8386 bp
  65% of sequence in fragments shorter than    9334 bp
  70% of sequence in fragments shorter than   10410 bp
  75% of sequence in fragments shorter than   11664 bp
  80% of sequence in fragments shorter than   13163 bp
  85% of sequence in fragments shorter than   15080 bp
  90% of sequence in fragments shorter than   17720 bp
  95% of sequence in fragments shorter than   22401 bp

# NdeI.SalI (CATATG+GTCGAC) double digest, 934 thousand fragments (903360+31396 sites), 3189 bp average fragment length
   5% of sequence in fragments shorter than    1075 bp
  10% of sequence in fragments shorter than    1647 bp
  15% of sequence in fragments shorter than    2188 bp
  20% of sequence in fragments shorter than    2703 bp
  25% of sequence in fragments shorter than    3197 bp
  30% of sequence in fragments shorter than    3703 bp
  35% of sequence in fragments shorter than    4216 bp
  40% of sequence in fragments shorter than    4742 bp
  45% of sequence in fragments shorter than    5287 bp
  50% of sequence in fragments shorter than    5876 bp
  55% of sequence in fragments shorter than    6516 bp
  60% of sequence in fragments shorter than    7224 bp
  65% of sequence in fragments shorter than    8029 bp
  70% of sequence in fragments shorter than    8968 bp
  75% of sequence in fragments shorter than   10088 bp
  80% of sequence in fragments shorter than   11480 bp
  85% of sequence in fragments shorter than   13398 bp
  90% of sequence in fragments shorter than   16365 bp
  95% of sequence in fragments shorter than   23039 bp

# BglII.PacI (AGATCT+TTAATTAA) double digest, 933 thousand fragments (774732+159455 sites), 3191 bp average fragment length
   5% of sequence in fragments shorter than    1124 bp
  10% of sequence in fragments shorter than    1674 bp
  15% of sequence in fragments shorter than    2172 bp
  20% of sequence in fragments shorter than    2639 bp
  25% of sequence in fragments shorter than    3101 bp
  30% of sequence in fragments shorter than    3557 bp
  35% of sequence in fragments shorter than    4022 bp
  40% of sequence in fragments shorter than    4495 bp
  45% of sequence in fragments shorter than    4991 bp
  50% of sequence in fragments shorter than    5517 bp
  55% of sequence in fragments shorter than    6092 bp
  60% of sequence in fragments shorter than    6725 bp
  65% of sequence in fragments shorter than    7427 bp
  70% of sequence in fragments shorter than    8226 bp
  75% of sequence in fragments shorter than    9163 bp
  80% of sequence in fragments shorter than   10337 bp
  85% of sequence in fragments shorter than   11879 bp
  90% of sequence in fragments shorter than   14210 bp
  95% of sequence in fragments shorter than   19071 bp

# ClaI.EcoRI (ATCGAT+GAATTC) double digest, 933 thousand fragments (86477+847401 sites), 3192 bp average fragment length
   5% of sequence in fragments shorter than    1083 bp
  10% of sequence in fragments shorter than    1712 bp
  15% of sequence in fragments shorter than    2249 bp
  20% of sequence in fragments shorter than    2745 bp
  25% of sequence in fragments shorter than    3224 bp
  30% of sequence in fragments shorter than    3693 bp
  35% of sequence in fragments shorter than    4180 bp
  40% of sequence in fragments shorter than    4681 bp
  45% of sequence in fragments shorter than    5206 bp
  50% of sequence in fragments shorter than    5758 bp
  55% of sequence in fragments shorter than    6351 bp
  60% of sequence in fragments shorter than    7006 bp
  65% of sequence in fragments shorter than    7723 bp
  70% of sequence in fragments shorter than    8538 bp
  75% of sequence in fragments shorter than    9495 bp
  80% of sequence in fragments shorter than   10661 bp
  85% of sequence in fragments shorter than   12149 bp
  90% of sequence in fragments shorter than   14285 bp
  95% of sequence in fragments shorter than   18109 bp

# HpaI.ScaI (GTTAAC+AGTACT) double digest, 932 thousand fragments (389418+543793 sites), 3194 bp average fragment length
   5% of sequence in fragments shorter than    1105 bp
  10% of sequence in fragments shorter than    1670 bp
  15% of sequence in fragments shorter than    2171 bp
  20% of sequence in fragments shorter than    2641 bp
  25% of sequence in fragments shorter than    3101 bp
  30% of sequence in fragments shorter than    3565 bp
  35% of sequence in fragments shorter than    4034 bp
  40% of sequence in fragments shorter than    4523 bp
  45% of sequence in fragments shorter than    5042 bp
  50% of sequence in fragments shorter than    5601 bp
  55% of sequence in fragments shorter than    6207 bp
  60% of sequence in fragments shorter than    6878 bp
  65% of sequence in fragments shorter than    7630 bp
  70% of sequence in fragments shorter than    8487 bp
  75% of sequence in fragments shorter than    9522 bp
  80% of sequence in fragments shorter than   10787 bp
  85% of sequence in fragments shorter than   12492 bp
  90% of sequence in fragments shorter than   15164 bp
  95% of sequence in fragments shorter than   20993 bp

# BglII.Hpy99I (AGATCT+CGWCG) double digest, 932 thousand fragments (774732+157917 sites), 3196 bp average fragment length
   5% of sequence in fragments shorter than    1122 bp
  10% of sequence in fragments shorter than    1693 bp
  15% of sequence in fragments shorter than    2202 bp
  20% of sequence in fragments shorter than    2680 bp
  25% of sequence in fragments shorter than    3153 bp
  30% of sequence in fragments shorter than    3619 bp
  35% of sequence in fragments shorter than    4092 bp
  40% of sequence in fragments shorter than    4572 bp
  45% of sequence in fragments shorter than    5075 bp
  50% of sequence in fragments shorter than    5606 bp
  55% of sequence in fragments shorter than    6178 bp
  60% of sequence in fragments shorter than    6802 bp
  65% of sequence in fragments shorter than    7499 bp
  70% of sequence in fragments shorter than    8275 bp
  75% of sequence in fragments shorter than    9195 bp
  80% of sequence in fragments shorter than   10318 bp
  85% of sequence in fragments shorter than   11727 bp
  90% of sequence in fragments shorter than   13849 bp
  95% of sequence in fragments shorter than   17838 bp

# AclI.NcoI (AACGTT+CCATGG) double digest, 931 thousand fragments (172706+759594 sites), 3197 bp average fragment length
   5% of sequence in fragments shorter than    1094 bp
  10% of sequence in fragments shorter than    1680 bp
  15% of sequence in fragments shorter than    2193 bp
  20% of sequence in fragments shorter than    2682 bp
  25% of sequence in fragments shorter than    3155 bp
  30% of sequence in fragments shorter than    3619 bp
  35% of sequence in fragments shorter than    4100 bp
  40% of sequence in fragments shorter than    4580 bp
  45% of sequence in fragments shorter than    5099 bp
  50% of sequence in fragments shorter than    5643 bp
  55% of sequence in fragments shorter than    6222 bp
  60% of sequence in fragments shorter than    6866 bp
  65% of sequence in fragments shorter than    7589 bp
  70% of sequence in fragments shorter than    8415 bp
  75% of sequence in fragments shorter than    9377 bp
  80% of sequence in fragments shorter than   10556 bp
  85% of sequence in fragments shorter than   12090 bp
  90% of sequence in fragments shorter than   14298 bp
  95% of sequence in fragments shorter than   18378 bp

# BceAI.SwaI (ACGGC+ATTTAAAT) double digest, 931 thousand fragments (705178+226602 sites), 3199 bp average fragment length
   5% of sequence in fragments shorter than    1087 bp
  10% of sequence in fragments shorter than    1719 bp
  15% of sequence in fragments shorter than    2286 bp
  20% of sequence in fragments shorter than    2829 bp
  25% of sequence in fragments shorter than    3363 bp
  30% of sequence in fragments shorter than    3898 bp
  35% of sequence in fragments shorter than    4451 bp
  40% of sequence in fragments shorter than    5029 bp
  45% of sequence in fragments shorter than    5632 bp
  50% of sequence in fragments shorter than    6262 bp
  55% of sequence in fragments shorter than    6955 bp
  60% of sequence in fragments shorter than    7709 bp
  65% of sequence in fragments shorter than    8548 bp
  70% of sequence in fragments shorter than    9501 bp
  75% of sequence in fragments shorter than   10611 bp
  80% of sequence in fragments shorter than   11967 bp
  85% of sequence in fragments shorter than   13704 bp
  90% of sequence in fragments shorter than   16202 bp
  95% of sequence in fragments shorter than   20928 bp

# BciVI.BsrBI (GTATCC+CCGCTC) double digest, 931 thousand fragments (696093+235523 sites), 3200 bp average fragment length
   5% of sequence in fragments shorter than    1139 bp
  10% of sequence in fragments shorter than    1721 bp
  15% of sequence in fragments shorter than    2213 bp
  20% of sequence in fragments shorter than    2702 bp
  25% of sequence in fragments shorter than    3181 bp
  30% of sequence in fragments shorter than    3655 bp
  35% of sequence in fragments shorter than    4137 bp
  40% of sequence in fragments shorter than    4626 bp
  45% of sequence in fragments shorter than    5143 bp
  50% of sequence in fragments shorter than    5692 bp
  55% of sequence in fragments shorter than    6273 bp
  60% of sequence in fragments shorter than    6903 bp
  65% of sequence in fragments shorter than    7607 bp
  70% of sequence in fragments shorter than    8404 bp
  75% of sequence in fragments shorter than    9326 bp
  80% of sequence in fragments shorter than   10435 bp
  85% of sequence in fragments shorter than   11861 bp
  90% of sequence in fragments shorter than   13872 bp
  95% of sequence in fragments shorter than   17523 bp

# AatII.HindIII (GACGTC+AAGCTT) double digest, 931 thousand fragments (71089+860365 sites), 3200 bp average fragment length
   5% of sequence in fragments shorter than    1125 bp
  10% of sequence in fragments shorter than    1712 bp
  15% of sequence in fragments shorter than    2172 bp
  20% of sequence in fragments shorter than    2643 bp
  25% of sequence in fragments shorter than    3094 bp
  30% of sequence in fragments shorter than    3555 bp
  35% of sequence in fragments shorter than    4020 bp
  40% of sequence in fragments shorter than    4497 bp
  45% of sequence in fragments shorter than    4993 bp
  50% of sequence in fragments shorter than    5519 bp
  55% of sequence in fragments shorter than    6088 bp
  60% of sequence in fragments shorter than    6711 bp
  65% of sequence in fragments shorter than    7405 bp
  70% of sequence in fragments shorter than    8192 bp
  75% of sequence in fragments shorter than    9098 bp
  80% of sequence in fragments shorter than   10210 bp
  85% of sequence in fragments shorter than   11627 bp
  90% of sequence in fragments shorter than   13693 bp
  95% of sequence in fragments shorter than   17572 bp

# AflII.KpnI (CTTAAG+GGTACC) double digest, 930 thousand fragments (643284+287974 sites), 3201 bp average fragment length
   5% of sequence in fragments shorter than    1107 bp
  10% of sequence in fragments shorter than    1669 bp
  15% of sequence in fragments shorter than    2163 bp
  20% of sequence in fragments shorter than    2630 bp
  25% of sequence in fragments shorter than    3087 bp
  30% of sequence in fragments shorter than    3541 bp
  35% of sequence in fragments shorter than    4005 bp
  40% of sequence in fragments shorter than    4481 bp
  45% of sequence in fragments shorter than    4978 bp
  50% of sequence in fragments shorter than    5514 bp
  55% of sequence in fragments shorter than    6086 bp
  60% of sequence in fragments shorter than    6708 bp
  65% of sequence in fragments shorter than    7401 bp
  70% of sequence in fragments shorter than    8198 bp
  75% of sequence in fragments shorter than    9120 bp
  80% of sequence in fragments shorter than   10252 bp
  85% of sequence in fragments shorter than   11738 bp
  90% of sequence in fragments shorter than   13987 bp
  95% of sequence in fragments shorter than   18622 bp

# AvrII.BstEII (CCTAGG+GGTNACC) double digest, 930 thousand fragments (594956+335865 sites), 3202 bp average fragment length
   5% of sequence in fragments shorter than    1111 bp
  10% of sequence in fragments shorter than    1708 bp
  15% of sequence in fragments shorter than    2219 bp
  20% of sequence in fragments shorter than    2690 bp
  25% of sequence in fragments shorter than    3117 bp
  30% of sequence in fragments shorter than    3575 bp
  35% of sequence in fragments shorter than    4054 bp
  40% of sequence in fragments shorter than    4550 bp
  45% of sequence in fragments shorter than    5072 bp
  50% of sequence in fragments shorter than    5629 bp
  55% of sequence in fragments shorter than    6233 bp
  60% of sequence in fragments shorter than    6892 bp
  65% of sequence in fragments shorter than    7631 bp
  70% of sequence in fragments shorter than    8458 bp
  75% of sequence in fragments shorter than    9441 bp
  80% of sequence in fragments shorter than   10645 bp
  85% of sequence in fragments shorter than   12233 bp
  90% of sequence in fragments shorter than   14626 bp
  95% of sequence in fragments shorter than   19515 bp

# AflII.NheI (CTTAAG+GCTAGC) double digest, 930 thousand fragments (643284+287384 sites), 3203 bp average fragment length
   5% of sequence in fragments shorter than    1096 bp
  10% of sequence in fragments shorter than    1682 bp
  15% of sequence in fragments shorter than    2171 bp
  20% of sequence in fragments shorter than    2637 bp
  25% of sequence in fragments shorter than    3074 bp
  30% of sequence in fragments shorter than    3541 bp
  35% of sequence in fragments shorter than    4015 bp
  40% of sequence in fragments shorter than    4508 bp
  45% of sequence in fragments shorter than    5016 bp
  50% of sequence in fragments shorter than    5558 bp
  55% of sequence in fragments shorter than    6147 bp
  60% of sequence in fragments shorter than    6789 bp
  65% of sequence in fragments shorter than    7504 bp
  70% of sequence in fragments shorter than    8324 bp
  75% of sequence in fragments shorter than    9289 bp
  80% of sequence in fragments shorter than   10476 bp
  85% of sequence in fragments shorter than   12028 bp
  90% of sequence in fragments shorter than   14326 bp
  95% of sequence in fragments shorter than   19080 bp

# AscI.StuI (GGCGCGCC+AGGCCT) double digest, 930 thousand fragments (4751+925729 sites), 3204 bp average fragment length
   5% of sequence in fragments shorter than     960 bp
  10% of sequence in fragments shorter than    1641 bp
  15% of sequence in fragments shorter than    2257 bp
  20% of sequence in fragments shorter than    2880 bp
  25% of sequence in fragments shorter than    3483 bp
  30% of sequence in fragments shorter than    4092 bp
  35% of sequence in fragments shorter than    4717 bp
  40% of sequence in fragments shorter than    5374 bp
  45% of sequence in fragments shorter than    6052 bp
  50% of sequence in fragments shorter than    6787 bp
  55% of sequence in fragments shorter than    7578 bp
  60% of sequence in fragments shorter than    8421 bp
  65% of sequence in fragments shorter than    9375 bp
  70% of sequence in fragments shorter than   10457 bp
  75% of sequence in fragments shorter than   11716 bp
  80% of sequence in fragments shorter than   13214 bp
  85% of sequence in fragments shorter than   15137 bp
  90% of sequence in fragments shorter than   17781 bp
  95% of sequence in fragments shorter than   22477 bp

# BamHI.MfeI (GGATCC+CAATTG) double digest, 929 thousand fragments (365999+564303 sites), 3204 bp average fragment length
   5% of sequence in fragments shorter than    1108 bp
  10% of sequence in fragments shorter than    1701 bp
  15% of sequence in fragments shorter than    2182 bp
  20% of sequence in fragments shorter than    2655 bp
  25% of sequence in fragments shorter than    3107 bp
  30% of sequence in fragments shorter than    3565 bp
  35% of sequence in fragments shorter than    4028 bp
  40% of sequence in fragments shorter than    4502 bp
  45% of sequence in fragments shorter than    4989 bp
  50% of sequence in fragments shorter than    5505 bp
  55% of sequence in fragments shorter than    6062 bp
  60% of sequence in fragments shorter than    6668 bp
  65% of sequence in fragments shorter than    7341 bp
  70% of sequence in fragments shorter than    8101 bp
  75% of sequence in fragments shorter than    8992 bp
  80% of sequence in fragments shorter than   10073 bp
  85% of sequence in fragments shorter than   11468 bp
  90% of sequence in fragments shorter than   13482 bp
  95% of sequence in fragments shorter than   17213 bp

# SapI.SphI (GCTCTTC+GCATGC) double digest, 927 thousand fragments (377161+550952 sites), 3212 bp average fragment length
   5% of sequence in fragments shorter than    1110 bp
  10% of sequence in fragments shorter than    1686 bp
  15% of sequence in fragments shorter than    2192 bp
  20% of sequence in fragments shorter than    2667 bp
  25% of sequence in fragments shorter than    3131 bp
  30% of sequence in fragments shorter than    3598 bp
  35% of sequence in fragments shorter than    4067 bp
  40% of sequence in fragments shorter than    4556 bp
  45% of sequence in fragments shorter than    5068 bp
  50% of sequence in fragments shorter than    5612 bp
  55% of sequence in fragments shorter than    6193 bp
  60% of sequence in fragments shorter than    6826 bp
  65% of sequence in fragments shorter than    7530 bp
  70% of sequence in fragments shorter than    8339 bp
  75% of sequence in fragments shorter than    9281 bp
  80% of sequence in fragments shorter than   10456 bp
  85% of sequence in fragments shorter than   11990 bp
  90% of sequence in fragments shorter than   14207 bp
  95% of sequence in fragments shorter than   18586 bp

# HaeII.SexAI (RGCGCY+ACCWGGT) double digest, 927 thousand fragments (630101+297900 sites), 3212 bp average fragment length
   5% of sequence in fragments shorter than     995 bp
  10% of sequence in fragments shorter than    1708 bp
  15% of sequence in fragments shorter than    2357 bp
  20% of sequence in fragments shorter than    3012 bp
  25% of sequence in fragments shorter than    3659 bp
  30% of sequence in fragments shorter than    4319 bp
  35% of sequence in fragments shorter than    5007 bp
  40% of sequence in fragments shorter than    5726 bp
  45% of sequence in fragments shorter than    6475 bp
  50% of sequence in fragments shorter than    7297 bp
  55% of sequence in fragments shorter than    8188 bp
  60% of sequence in fragments shorter than    9152 bp
  65% of sequence in fragments shorter than   10242 bp
  70% of sequence in fragments shorter than   11495 bp
  75% of sequence in fragments shorter than   12962 bp
  80% of sequence in fragments shorter than   14772 bp
  85% of sequence in fragments shorter than   17103 bp
  90% of sequence in fragments shorter than   20375 bp
  95% of sequence in fragments shorter than   26011 bp

# SmaI.SphI (CCCGGG+GCATGC) double digest, 927 thousand fragments (376940+550952 sites), 3212 bp average fragment length
   5% of sequence in fragments shorter than    1079 bp
  10% of sequence in fragments shorter than    1680 bp
  15% of sequence in fragments shorter than    2215 bp
  20% of sequence in fragments shorter than    2736 bp
  25% of sequence in fragments shorter than    3266 bp
  30% of sequence in fragments shorter than    3804 bp
  35% of sequence in fragments shorter than    4367 bp
  40% of sequence in fragments shorter than    4964 bp
  45% of sequence in fragments shorter than    5594 bp
  50% of sequence in fragments shorter than    6275 bp
  55% of sequence in fragments shorter than    7019 bp
  60% of sequence in fragments shorter than    7846 bp
  65% of sequence in fragments shorter than    8782 bp
  70% of sequence in fragments shorter than    9870 bp
  75% of sequence in fragments shorter than   11184 bp
  80% of sequence in fragments shorter than   12769 bp
  85% of sequence in fragments shorter than   14857 bp
  90% of sequence in fragments shorter than   17934 bp
  95% of sequence in fragments shorter than   24142 bp

# HindIII.SacII (AAGCTT+CCGCGG) double digest, 926 thousand fragments (860365+66901 sites), 3215 bp average fragment length
   5% of sequence in fragments shorter than    1133 bp
  10% of sequence in fragments shorter than    1739 bp
  15% of sequence in fragments shorter than    2216 bp
  20% of sequence in fragments shorter than    2703 bp
  25% of sequence in fragments shorter than    3171 bp
  30% of sequence in fragments shorter than    3646 bp
  35% of sequence in fragments shorter than    4124 bp
  40% of sequence in fragments shorter than    4614 bp
  45% of sequence in fragments shorter than    5124 bp
  50% of sequence in fragments shorter than    5670 bp
  55% of sequence in fragments shorter than    6258 bp
  60% of sequence in fragments shorter than    6893 bp
  65% of sequence in fragments shorter than    7601 bp
  70% of sequence in fragments shorter than    8408 bp
  75% of sequence in fragments shorter than    9335 bp
  80% of sequence in fragments shorter than   10469 bp
  85% of sequence in fragments shorter than   11937 bp
  90% of sequence in fragments shorter than   14044 bp
  95% of sequence in fragments shorter than   18007 bp

# BciVI.KasI (GTATCC+GGCGCC) double digest, 926 thousand fragments (696093+230381 sites), 3217 bp average fragment length
   5% of sequence in fragments shorter than    1140 bp
  10% of sequence in fragments shorter than    1723 bp
  15% of sequence in fragments shorter than    2194 bp
  20% of sequence in fragments shorter than    2672 bp
  25% of sequence in fragments shorter than    3134 bp
  30% of sequence in fragments shorter than    3599 bp
  35% of sequence in fragments shorter than    4060 bp
  40% of sequence in fragments shorter than    4541 bp
  45% of sequence in fragments shorter than    5050 bp
  50% of sequence in fragments shorter than    5588 bp
  55% of sequence in fragments shorter than    6159 bp
  60% of sequence in fragments shorter than    6779 bp
  65% of sequence in fragments shorter than    7472 bp
  70% of sequence in fragments shorter than    8254 bp
  75% of sequence in fragments shorter than    9157 bp
  80% of sequence in fragments shorter than   10256 bp
  85% of sequence in fragments shorter than   11652 bp
  90% of sequence in fragments shorter than   13676 bp
  95% of sequence in fragments shorter than   17466 bp

# MluI.NdeI (ACGCGT+CATATG) double digest, 924 thousand fragments (21415+903360 sites), 3223 bp average fragment length
   5% of sequence in fragments shorter than    1087 bp
  10% of sequence in fragments shorter than    1669 bp
  15% of sequence in fragments shorter than    2217 bp
  20% of sequence in fragments shorter than    2738 bp
  25% of sequence in fragments shorter than    3239 bp
  30% of sequence in fragments shorter than    3752 bp
  35% of sequence in fragments shorter than    4274 bp
  40% of sequence in fragments shorter than    4809 bp
  45% of sequence in fragments shorter than    5358 bp
  50% of sequence in fragments shorter than    5953 bp
  55% of sequence in fragments shorter than    6595 bp
  60% of sequence in fragments shorter than    7310 bp
  65% of sequence in fragments shorter than    8118 bp
  70% of sequence in fragments shorter than    9062 bp
  75% of sequence in fragments shorter than   10179 bp
  80% of sequence in fragments shorter than   11580 bp
  85% of sequence in fragments shorter than   13494 bp
  90% of sequence in fragments shorter than   16433 bp
  95% of sequence in fragments shorter than   23076 bp

# BciVI.SwaI (GTATCC+ATTTAAAT) double digest, 922 thousand fragments (696093+226602 sites), 3231 bp average fragment length
   5% of sequence in fragments shorter than    1140 bp
  10% of sequence in fragments shorter than    1721 bp
  15% of sequence in fragments shorter than    2193 bp
  20% of sequence in fragments shorter than    2664 bp
  25% of sequence in fragments shorter than    3124 bp
  30% of sequence in fragments shorter than    3586 bp
  35% of sequence in fragments shorter than    4055 bp
  40% of sequence in fragments shorter than    4537 bp
  45% of sequence in fragments shorter than    5040 bp
  50% of sequence in fragments shorter than    5580 bp
  55% of sequence in fragments shorter than    6164 bp
  60% of sequence in fragments shorter than    6801 bp
  65% of sequence in fragments shorter than    7517 bp
  70% of sequence in fragments shorter than    8343 bp
  75% of sequence in fragments shorter than    9312 bp
  80% of sequence in fragments shorter than   10503 bp
  85% of sequence in fragments shorter than   12058 bp
  90% of sequence in fragments shorter than   14430 bp
  95% of sequence in fragments shorter than   19141 bp

# AatII.XbaI (GACGTC+TCTAGA) double digest, 921 thousand fragments (71089+850998 sites), 3233 bp average fragment length
   5% of sequence in fragments shorter than    1100 bp
  10% of sequence in fragments shorter than    1679 bp
  15% of sequence in fragments shorter than    2196 bp
  20% of sequence in fragments shorter than    2668 bp
  25% of sequence in fragments shorter than    3148 bp
  30% of sequence in fragments shorter than    3629 bp
  35% of sequence in fragments shorter than    4111 bp
  40% of sequence in fragments shorter than    4609 bp
  45% of sequence in fragments shorter than    5129 bp
  50% of sequence in fragments shorter than    5675 bp
  55% of sequence in fragments shorter than    6268 bp
  60% of sequence in fragments shorter than    6907 bp
  65% of sequence in fragments shorter than    7616 bp
  70% of sequence in fragments shorter than    8415 bp
  75% of sequence in fragments shorter than    9345 bp
  80% of sequence in fragments shorter than   10482 bp
  85% of sequence in fragments shorter than   11949 bp
  90% of sequence in fragments shorter than   13980 bp
  95% of sequence in fragments shorter than   17573 bp

# SapI.ScaI (GCTCTTC+AGTACT) double digest, 920 thousand fragments (377161+543793 sites), 3237 bp average fragment length
   5% of sequence in fragments shorter than    1129 bp
  10% of sequence in fragments shorter than    1706 bp
  15% of sequence in fragments shorter than    2209 bp
  20% of sequence in fragments shorter than    2677 bp
  25% of sequence in fragments shorter than    3133 bp
  30% of sequence in fragments shorter than    3593 bp
  35% of sequence in fragments shorter than    4055 bp
  40% of sequence in fragments shorter than    4529 bp
  45% of sequence in fragments shorter than    5016 bp
  50% of sequence in fragments shorter than    5541 bp
  55% of sequence in fragments shorter than    6106 bp
  60% of sequence in fragments shorter than    6724 bp
  65% of sequence in fragments shorter than    7413 bp
  70% of sequence in fragments shorter than    8174 bp
  75% of sequence in fragments shorter than    9082 bp
  80% of sequence in fragments shorter than   10193 bp
  85% of sequence in fragments shorter than   11664 bp
  90% of sequence in fragments shorter than   13778 bp
  95% of sequence in fragments shorter than   17994 bp

# ScaI.SmaI (AGTACT+CCCGGG) double digest, 920 thousand fragments (543793+376940 sites), 3237 bp average fragment length
   5% of sequence in fragments shorter than    1109 bp
  10% of sequence in fragments shorter than    1698 bp
  15% of sequence in fragments shorter than    2215 bp
  20% of sequence in fragments shorter than    2704 bp
  25% of sequence in fragments shorter than    3189 bp
  30% of sequence in fragments shorter than    3681 bp
  35% of sequence in fragments shorter than    4185 bp
  40% of sequence in fragments shorter than    4705 bp
  45% of sequence in fragments shorter than    5255 bp
  50% of sequence in fragments shorter than    5846 bp
  55% of sequence in fragments shorter than    6486 bp
  60% of sequence in fragments shorter than    7186 bp
  65% of sequence in fragments shorter than    7967 bp
  70% of sequence in fragments shorter than    8859 bp
  75% of sequence in fragments shorter than    9899 bp
  80% of sequence in fragments shorter than   11197 bp
  85% of sequence in fragments shorter than   12871 bp
  90% of sequence in fragments shorter than   15363 bp
  95% of sequence in fragments shorter than   20549 bp

# HindIII.PspXI (AAGCTT+VCTCGAGB) double digest, 919 thousand fragments (860365+59193 sites), 3242 bp average fragment length
   5% of sequence in fragments shorter than    1136 bp
  10% of sequence in fragments shorter than    1731 bp
  15% of sequence in fragments shorter than    2196 bp
  20% of sequence in fragments shorter than    2674 bp
  25% of sequence in fragments shorter than    3134 bp
  30% of sequence in fragments shorter than    3602 bp
  35% of sequence in fragments shorter than    4068 bp
  40% of sequence in fragments shorter than    4551 bp
  45% of sequence in fragments shorter than    5053 bp
  50% of sequence in fragments shorter than    5589 bp
  55% of sequence in fragments shorter than    6171 bp
  60% of sequence in fragments shorter than    6799 bp
  65% of sequence in fragments shorter than    7492 bp
  70% of sequence in fragments shorter than    8288 bp
  75% of sequence in fragments shorter than    9207 bp
  80% of sequence in fragments shorter than   10327 bp
  85% of sequence in fragments shorter than   11783 bp
  90% of sequence in fragments shorter than   13854 bp
  95% of sequence in fragments shorter than   17805 bp

# NcoI.PacI (CCATGG+TTAATTAA) double digest, 918 thousand fragments (759594+159455 sites), 3243 bp average fragment length
   5% of sequence in fragments shorter than    1145 bp
  10% of sequence in fragments shorter than    1735 bp
  15% of sequence in fragments shorter than    2258 bp
  20% of sequence in fragments shorter than    2748 bp
  25% of sequence in fragments shorter than    3227 bp
  30% of sequence in fragments shorter than    3708 bp
  35% of sequence in fragments shorter than    4196 bp
  40% of sequence in fragments shorter than    4678 bp
  45% of sequence in fragments shorter than    5205 bp
  50% of sequence in fragments shorter than    5747 bp
  55% of sequence in fragments shorter than    6328 bp
  60% of sequence in fragments shorter than    6973 bp
  65% of sequence in fragments shorter than    7698 bp
  70% of sequence in fragments shorter than    8521 bp
  75% of sequence in fragments shorter than    9483 bp
  80% of sequence in fragments shorter than   10667 bp
  85% of sequence in fragments shorter than   12214 bp
  90% of sequence in fragments shorter than   14432 bp
  95% of sequence in fragments shorter than   18845 bp

# NdeI.SgrAI (CATATG+CRCCGGYG) double digest, 918 thousand fragments (903360+15270 sites), 3245 bp average fragment length
   5% of sequence in fragments shorter than    1094 bp
  10% of sequence in fragments shorter than    1680 bp
  15% of sequence in fragments shorter than    2230 bp
  20% of sequence in fragments shorter than    2754 bp
  25% of sequence in fragments shorter than    3259 bp
  30% of sequence in fragments shorter than    3775 bp
  35% of sequence in fragments shorter than    4303 bp
  40% of sequence in fragments shorter than    4838 bp
  45% of sequence in fragments shorter than    5393 bp
  50% of sequence in fragments shorter than    5991 bp
  55% of sequence in fragments shorter than    6639 bp
  60% of sequence in fragments shorter than    7361 bp
  65% of sequence in fragments shorter than    8185 bp
  70% of sequence in fragments shorter than    9141 bp
  75% of sequence in fragments shorter than   10270 bp
  80% of sequence in fragments shorter than   11695 bp
  85% of sequence in fragments shorter than   13647 bp
  90% of sequence in fragments shorter than   16655 bp
  95% of sequence in fragments shorter than   23342 bp

# AatII.EcoRI (GACGTC+GAATTC) double digest, 918 thousand fragments (71089+847401 sites), 3246 bp average fragment length
   5% of sequence in fragments shorter than    1107 bp
  10% of sequence in fragments shorter than    1747 bp
  15% of sequence in fragments shorter than    2293 bp
  20% of sequence in fragments shorter than    2791 bp
  25% of sequence in fragments shorter than    3279 bp
  30% of sequence in fragments shorter than    3754 bp
  35% of sequence in fragments shorter than    4246 bp
  40% of sequence in fragments shorter than    4750 bp
  45% of sequence in fragments shorter than    5281 bp
  50% of sequence in fragments shorter than    5840 bp
  55% of sequence in fragments shorter than    6439 bp
  60% of sequence in fragments shorter than    7093 bp
  65% of sequence in fragments shorter than    7818 bp
  70% of sequence in fragments shorter than    8626 bp
  75% of sequence in fragments shorter than    9563 bp
  80% of sequence in fragments shorter than   10693 bp
  85% of sequence in fragments shorter than   12144 bp
  90% of sequence in fragments shorter than   14193 bp
  95% of sequence in fragments shorter than   17784 bp

# NdeI.NruI (CATATG+TCGCGA) double digest, 917 thousand fragments (903360+14872 sites), 3246 bp average fragment length
   5% of sequence in fragments shorter than    1092 bp
  10% of sequence in fragments shorter than    1678 bp
  15% of sequence in fragments shorter than    2228 bp
  20% of sequence in fragments shorter than    2753 bp
  25% of sequence in fragments shorter than    3261 bp
  30% of sequence in fragments shorter than    3778 bp
  35% of sequence in fragments shorter than    4306 bp
  40% of sequence in fragments shorter than    4842 bp
  45% of sequence in fragments shorter than    5398 bp
  50% of sequence in fragments shorter than    6000 bp
  55% of sequence in fragments shorter than    6652 bp
  60% of sequence in fragments shorter than    7378 bp
  65% of sequence in fragments shorter than    8208 bp
  70% of sequence in fragments shorter than    9167 bp
  75% of sequence in fragments shorter than   10312 bp
  80% of sequence in fragments shorter than   11749 bp
  85% of sequence in fragments shorter than   13736 bp
  90% of sequence in fragments shorter than   16818 bp
  95% of sequence in fragments shorter than   23790 bp

# HaeII.KpnI (RGCGCY+GGTACC) double digest, 917 thousand fragments (630101+287974 sites), 3247 bp average fragment length
   5% of sequence in fragments shorter than    1032 bp
  10% of sequence in fragments shorter than    1692 bp
  15% of sequence in fragments shorter than    2357 bp
  20% of sequence in fragments shorter than    3007 bp
  25% of sequence in fragments shorter than    3649 bp
  30% of sequence in fragments shorter than    4304 bp
  35% of sequence in fragments shorter than    4986 bp
  40% of sequence in fragments shorter than    5694 bp
  45% of sequence in fragments shorter than    6435 bp
  50% of sequence in fragments shorter than    7223 bp
  55% of sequence in fragments shorter than    8090 bp
  60% of sequence in fragments shorter than    9038 bp
  65% of sequence in fragments shorter than   10100 bp
  70% of sequence in fragments shorter than   11310 bp
  75% of sequence in fragments shorter than   12726 bp
  80% of sequence in fragments shorter than   14453 bp
  85% of sequence in fragments shorter than   16670 bp
  90% of sequence in fragments shorter than   19792 bp
  95% of sequence in fragments shorter than   25165 bp

# SacII.XbaI (CCGCGG+TCTAGA) double digest, 917 thousand fragments (66901+850998 sites), 3248 bp average fragment length
   5% of sequence in fragments shorter than    1109 bp
  10% of sequence in fragments shorter than    1708 bp
  15% of sequence in fragments shorter than    2234 bp
  20% of sequence in fragments shorter than    2731 bp
  25% of sequence in fragments shorter than    3225 bp
  30% of sequence in fragments shorter than    3717 bp
  35% of sequence in fragments shorter than    4217 bp
  40% of sequence in fragments shorter than    4729 bp
  45% of sequence in fragments shorter than    5258 bp
  50% of sequence in fragments shorter than    5820 bp
  55% of sequence in fragments shorter than    6423 bp
  60% of sequence in fragments shorter than    7082 bp
  65% of sequence in fragments shorter than    7802 bp
  70% of sequence in fragments shorter than    8612 bp
  75% of sequence in fragments shorter than    9572 bp
  80% of sequence in fragments shorter than   10742 bp
  85% of sequence in fragments shorter than   12228 bp
  90% of sequence in fragments shorter than   14290 bp
  95% of sequence in fragments shorter than   17920 bp

# NdeI.PvuI (CATATG+CGATCG) double digest, 917 thousand fragments (903360+14186 sites), 3249 bp average fragment length
   5% of sequence in fragments shorter than    1094 bp
  10% of sequence in fragments shorter than    1681 bp
  15% of sequence in fragments shorter than    2226 bp
  20% of sequence in fragments shorter than    2752 bp
  25% of sequence in fragments shorter than    3260 bp
  30% of sequence in fragments shorter than    3777 bp
  35% of sequence in fragments shorter than    4307 bp
  40% of sequence in fragments shorter than    4844 bp
  45% of sequence in fragments shorter than    5403 bp
  50% of sequence in fragments shorter than    6006 bp
  55% of sequence in fragments shorter than    6661 bp
  60% of sequence in fragments shorter than    7389 bp
  65% of sequence in fragments shorter than    8225 bp
  70% of sequence in fragments shorter than    9191 bp
  75% of sequence in fragments shorter than   10335 bp
  80% of sequence in fragments shorter than   11776 bp
  85% of sequence in fragments shorter than   13762 bp
  90% of sequence in fragments shorter than   16856 bp
  95% of sequence in fragments shorter than   23790 bp

# Hpy99I.NcoI (CGWCG+CCATGG) double digest, 917 thousand fragments (157917+759594 sites), 3249 bp average fragment length
   5% of sequence in fragments shorter than    1122 bp
  10% of sequence in fragments shorter than    1743 bp
  15% of sequence in fragments shorter than    2298 bp
  20% of sequence in fragments shorter than    2822 bp
  25% of sequence in fragments shorter than    3344 bp
  30% of sequence in fragments shorter than    3868 bp
  35% of sequence in fragments shorter than    4400 bp
  40% of sequence in fragments shorter than    4929 bp
  45% of sequence in fragments shorter than    5498 bp
  50% of sequence in fragments shorter than    6074 bp
  55% of sequence in fragments shorter than    6741 bp
  60% of sequence in fragments shorter than    7478 bp
  65% of sequence in fragments shorter than    8307 bp
  70% of sequence in fragments shorter than    9253 bp
  75% of sequence in fragments shorter than   10371 bp
  80% of sequence in fragments shorter than   11736 bp
  85% of sequence in fragments shorter than   13507 bp
  90% of sequence in fragments shorter than   16107 bp
  95% of sequence in fragments shorter than   21150 bp

# HaeII.NheI (RGCGCY+GCTAGC) double digest, 917 thousand fragments (630101+287384 sites), 3249 bp average fragment length
   5% of sequence in fragments shorter than    1035 bp
  10% of sequence in fragments shorter than    1761 bp
  15% of sequence in fragments shorter than    2398 bp
  20% of sequence in fragments shorter than    3033 bp
  25% of sequence in fragments shorter than    3685 bp
  30% of sequence in fragments shorter than    4345 bp
  35% of sequence in fragments shorter than    5028 bp
  40% of sequence in fragments shorter than    5734 bp
  45% of sequence in fragments shorter than    6484 bp
  50% of sequence in fragments shorter than    7276 bp
  55% of sequence in fragments shorter than    8146 bp
  60% of sequence in fragments shorter than    9093 bp
  65% of sequence in fragments shorter than   10143 bp
  70% of sequence in fragments shorter than   11343 bp
  75% of sequence in fragments shorter than   12748 bp
  80% of sequence in fragments shorter than   14422 bp
  85% of sequence in fragments shorter than   16583 bp
  90% of sequence in fragments shorter than   19627 bp
  95% of sequence in fragments shorter than   24840 bp

# FseI.NdeI (GGCCGGCC+CATATG) double digest, 916 thousand fragments (13662+903360 sites), 3251 bp average fragment length
   5% of sequence in fragments shorter than    1096 bp
  10% of sequence in fragments shorter than    1683 bp
  15% of sequence in fragments shorter than    2234 bp
  20% of sequence in fragments shorter than    2760 bp
  25% of sequence in fragments shorter than    3267 bp
  30% of sequence in fragments shorter than    3785 bp
  35% of sequence in fragments shorter than    4313 bp
  40% of sequence in fragments shorter than    4849 bp
  45% of sequence in fragments shorter than    5404 bp
  50% of sequence in fragments shorter than    6005 bp
  55% of sequence in fragments shorter than    6653 bp
  60% of sequence in fragments shorter than    7377 bp
  65% of sequence in fragments shorter than    8198 bp
  70% of sequence in fragments shorter than    9151 bp
  75% of sequence in fragments shorter than   10278 bp
  80% of sequence in fragments shorter than   11695 bp
  85% of sequence in fragments shorter than   13619 bp
  90% of sequence in fragments shorter than   16613 bp
  95% of sequence in fragments shorter than   23113 bp

# BamHI.SphI (GGATCC+GCATGC) double digest, 916 thousand fragments (365999+550952 sites), 3251 bp average fragment length
   5% of sequence in fragments shorter than    1140 bp
  10% of sequence in fragments shorter than    1722 bp
  15% of sequence in fragments shorter than    2218 bp
  20% of sequence in fragments shorter than    2694 bp
  25% of sequence in fragments shorter than    3164 bp
  30% of sequence in fragments shorter than    3640 bp
  35% of sequence in fragments shorter than    4124 bp
  40% of sequence in fragments shorter than    4615 bp
  45% of sequence in fragments shorter than    5137 bp
  50% of sequence in fragments shorter than    5682 bp
  55% of sequence in fragments shorter than    6278 bp
  60% of sequence in fragments shorter than    6928 bp
  65% of sequence in fragments shorter than    7657 bp
  70% of sequence in fragments shorter than    8490 bp
  75% of sequence in fragments shorter than    9478 bp
  80% of sequence in fragments shorter than   10676 bp
  85% of sequence in fragments shorter than   12264 bp
  90% of sequence in fragments shorter than   14540 bp
  95% of sequence in fragments shorter than   18920 bp

# EcoRI.SacII (GAATTC+CCGCGG) double digest, 913 thousand fragments (847401+66901 sites), 3260 bp average fragment length
   5% of sequence in fragments shorter than    1114 bp
  10% of sequence in fragments shorter than    1779 bp
  15% of sequence in fragments shorter than    2347 bp
  20% of sequence in fragments shorter than    2862 bp
  25% of sequence in fragments shorter than    3363 bp
  30% of sequence in fragments shorter than    3857 bp
  35% of sequence in fragments shorter than    4362 bp
  40% of sequence in fragments shorter than    4886 bp
  45% of sequence in fragments shorter than    5431 bp
  50% of sequence in fragments shorter than    5998 bp
  55% of sequence in fragments shorter than    6617 bp
  60% of sequence in fragments shorter than    7288 bp
  65% of sequence in fragments shorter than    8025 bp
  70% of sequence in fragments shorter than    8845 bp
  75% of sequence in fragments shorter than    9802 bp
  80% of sequence in fragments shorter than   10958 bp
  85% of sequence in fragments shorter than   12453 bp
  90% of sequence in fragments shorter than   14531 bp
  95% of sequence in fragments shorter than   18164 bp

# AgeI.HindIII (ACCGGT+AAGCTT) double digest, 913 thousand fragments (53386+860365 sites), 3262 bp average fragment length
   5% of sequence in fragments shorter than    1145 bp
  10% of sequence in fragments shorter than    1743 bp
  15% of sequence in fragments shorter than    2215 bp
  20% of sequence in fragments shorter than    2698 bp
  25% of sequence in fragments shorter than    3162 bp
  30% of sequence in fragments shorter than    3634 bp
  35% of sequence in fragments shorter than    4108 bp
  40% of sequence in fragments shorter than    4596 bp
  45% of sequence in fragments shorter than    5108 bp
  50% of sequence in fragments shorter than    5655 bp
  55% of sequence in fragments shorter than    6246 bp
  60% of sequence in fragments shorter than    6887 bp
  65% of sequence in fragments shorter than    7598 bp
  70% of sequence in fragments shorter than    8413 bp
  75% of sequence in fragments shorter than    9352 bp
  80% of sequence in fragments shorter than   10502 bp
  85% of sequence in fragments shorter than   12000 bp
  90% of sequence in fragments shorter than   14153 bp
  95% of sequence in fragments shorter than   18366 bp

# NdeI.NotI (CATATG+GCGGCCGC) double digest, 912 thousand fragments (903360+9795 sites), 3264 bp average fragment length
   5% of sequence in fragments shorter than    1099 bp
  10% of sequence in fragments shorter than    1689 bp
  15% of sequence in fragments shorter than    2244 bp
  20% of sequence in fragments shorter than    2773 bp
  25% of sequence in fragments shorter than    3283 bp
  30% of sequence in fragments shorter than    3805 bp
  35% of sequence in fragments shorter than    4335 bp
  40% of sequence in fragments shorter than    4874 bp
  45% of sequence in fragments shorter than    5435 bp
  50% of sequence in fragments shorter than    6041 bp
  55% of sequence in fragments shorter than    6698 bp
  60% of sequence in fragments shorter than    7429 bp
  65% of sequence in fragments shorter than    8259 bp
  70% of sequence in fragments shorter than    9229 bp
  75% of sequence in fragments shorter than   10375 bp
  80% of sequence in fragments shorter than   11818 bp
  85% of sequence in fragments shorter than   13817 bp
  90% of sequence in fragments shorter than   16915 bp
  95% of sequence in fragments shorter than   23797 bp

# NdeI.RsrII (CATATG+CGGWCCG) double digest, 912 thousand fragments (903360+9764 sites), 3264 bp average fragment length
   5% of sequence in fragments shorter than    1098 bp
  10% of sequence in fragments shorter than    1688 bp
  15% of sequence in fragments shorter than    2242 bp
  20% of sequence in fragments shorter than    2771 bp
  25% of sequence in fragments shorter than    3281 bp
  30% of sequence in fragments shorter than    3801 bp
  35% of sequence in fragments shorter than    4331 bp
  40% of sequence in fragments shorter than    4869 bp
  45% of sequence in fragments shorter than    5430 bp
  50% of sequence in fragments shorter than    6036 bp
  55% of sequence in fragments shorter than    6693 bp
  60% of sequence in fragments shorter than    7424 bp
  65% of sequence in fragments shorter than    8257 bp
  70% of sequence in fragments shorter than    9228 bp
  75% of sequence in fragments shorter than   10379 bp
  80% of sequence in fragments shorter than   11829 bp
  85% of sequence in fragments shorter than   13827 bp
  90% of sequence in fragments shorter than   16940 bp
  95% of sequence in fragments shorter than   23899 bp

# AclI.BclI (AACGTT+TGATCA) double digest, 911 thousand fragments (172706+738785 sites), 3270 bp average fragment length
   5% of sequence in fragments shorter than    1144 bp
  10% of sequence in fragments shorter than    1717 bp
  15% of sequence in fragments shorter than    2213 bp
  20% of sequence in fragments shorter than    2672 bp
  25% of sequence in fragments shorter than    3129 bp
  30% of sequence in fragments shorter than    3592 bp
  35% of sequence in fragments shorter than    4059 bp
  40% of sequence in fragments shorter than    4541 bp
  45% of sequence in fragments shorter than    5039 bp
  50% of sequence in fragments shorter than    5566 bp
  55% of sequence in fragments shorter than    6139 bp
  60% of sequence in fragments shorter than    6768 bp
  65% of sequence in fragments shorter than    7458 bp
  70% of sequence in fragments shorter than    8248 bp
  75% of sequence in fragments shorter than    9162 bp
  80% of sequence in fragments shorter than   10310 bp
  85% of sequence in fragments shorter than   11775 bp
  90% of sequence in fragments shorter than   13886 bp
  95% of sequence in fragments shorter than   18063 bp

# PspXI.XbaI (VCTCGAGB+TCTAGA) double digest, 909 thousand fragments (59193+850998 sites), 3275 bp average fragment length
   5% of sequence in fragments shorter than    1115 bp
  10% of sequence in fragments shorter than    1699 bp
  15% of sequence in fragments shorter than    2220 bp
  20% of sequence in fragments shorter than    2701 bp
  25% of sequence in fragments shorter than    3186 bp
  30% of sequence in fragments shorter than    3671 bp
  35% of sequence in fragments shorter than    4161 bp
  40% of sequence in fragments shorter than    4666 bp
  45% of sequence in fragments shorter than    5191 bp
  50% of sequence in fragments shorter than    5747 bp
  55% of sequence in fragments shorter than    6341 bp
  60% of sequence in fragments shorter than    6987 bp
  65% of sequence in fragments shorter than    7706 bp
  70% of sequence in fragments shorter than    8514 bp
  75% of sequence in fragments shorter than    9463 bp
  80% of sequence in fragments shorter than   10617 bp
  85% of sequence in fragments shorter than   12099 bp
  90% of sequence in fragments shorter than   14174 bp
  95% of sequence in fragments shorter than   17819 bp

# BamHI.ScaI (GGATCC+AGTACT) double digest, 909 thousand fragments (365999+543793 sites), 3276 bp average fragment length
   5% of sequence in fragments shorter than    1165 bp
  10% of sequence in fragments shorter than    1739 bp
  15% of sequence in fragments shorter than    2231 bp
  20% of sequence in fragments shorter than    2698 bp
  25% of sequence in fragments shorter than    3153 bp
  30% of sequence in fragments shorter than    3613 bp
  35% of sequence in fragments shorter than    4081 bp
  40% of sequence in fragments shorter than    4555 bp
  45% of sequence in fragments shorter than    5053 bp
  50% of sequence in fragments shorter than    5582 bp
  55% of sequence in fragments shorter than    6142 bp
  60% of sequence in fragments shorter than    6759 bp
  65% of sequence in fragments shorter than    7439 bp
  70% of sequence in fragments shorter than    8198 bp
  75% of sequence in fragments shorter than    9088 bp
  80% of sequence in fragments shorter than   10171 bp
  85% of sequence in fragments shorter than   11582 bp
  90% of sequence in fragments shorter than   13616 bp
  95% of sequence in fragments shorter than   17468 bp

# AscI.NdeI (GGCGCGCC+CATATG) double digest, 907 thousand fragments (4751+903360 sites), 3282 bp average fragment length
   5% of sequence in fragments shorter than    1102 bp
  10% of sequence in fragments shorter than    1696 bp
  15% of sequence in fragments shorter than    2253 bp
  20% of sequence in fragments shorter than    2786 bp
  25% of sequence in fragments shorter than    3299 bp
  30% of sequence in fragments shorter than    3826 bp
  35% of sequence in fragments shorter than    4361 bp
  40% of sequence in fragments shorter than    4904 bp
  45% of sequence in fragments shorter than    5471 bp
  50% of sequence in fragments shorter than    6083 bp
  55% of sequence in fragments shorter than    6749 bp
  60% of sequence in fragments shorter than    7496 bp
  65% of sequence in fragments shorter than    8340 bp
  70% of sequence in fragments shorter than    9330 bp
  75% of sequence in fragments shorter than   10507 bp
  80% of sequence in fragments shorter than   11999 bp
  85% of sequence in fragments shorter than   14059 bp
  90% of sequence in fragments shorter than   17280 bp
  95% of sequence in fragments shorter than   24673 bp

# BstEII.HgaI (GGTNACC+GACGC) double digest, 907 thousand fragments (335865+571889 sites), 3284 bp average fragment length
   5% of sequence in fragments shorter than    1095 bp
  10% of sequence in fragments shorter than    1752 bp
  15% of sequence in fragments shorter than    2382 bp
  20% of sequence in fragments shorter than    2988 bp
  25% of sequence in fragments shorter than    3595 bp
  30% of sequence in fragments shorter than    4212 bp
  35% of sequence in fragments shorter than    4848 bp
  40% of sequence in fragments shorter than    5509 bp
  45% of sequence in fragments shorter than    6215 bp
  50% of sequence in fragments shorter than    6970 bp
  55% of sequence in fragments shorter than    7787 bp
  60% of sequence in fragments shorter than    8703 bp
  65% of sequence in fragments shorter than    9725 bp
  70% of sequence in fragments shorter than   10905 bp
  75% of sequence in fragments shorter than   12282 bp
  80% of sequence in fragments shorter than   13974 bp
  85% of sequence in fragments shorter than   16188 bp
  90% of sequence in fragments shorter than   19403 bp
  95% of sequence in fragments shorter than   25572 bp

# BstEII.EciI (GGTNACC+GGCGGA) double digest, 906 thousand fragments (335865+571273 sites), 3286 bp average fragment length
   5% of sequence in fragments shorter than    1079 bp
  10% of sequence in fragments shorter than    1719 bp
  15% of sequence in fragments shorter than    2300 bp
  20% of sequence in fragments shorter than    2869 bp
  25% of sequence in fragments shorter than    3434 bp
  30% of sequence in fragments shorter than    4028 bp
  35% of sequence in fragments shorter than    4648 bp
  40% of sequence in fragments shorter than    5301 bp
  45% of sequence in fragments shorter than    5995 bp
  50% of sequence in fragments shorter than    6745 bp
  55% of sequence in fragments shorter than    7574 bp
  60% of sequence in fragments shorter than    8491 bp
  65% of sequence in fragments shorter than    9551 bp
  70% of sequence in fragments shorter than   10771 bp
  75% of sequence in fragments shorter than   12219 bp
  80% of sequence in fragments shorter than   13994 bp
  85% of sequence in fragments shorter than   16407 bp
  90% of sequence in fragments shorter than   19976 bp
  95% of sequence in fragments shorter than   27376 bp

# BglII.SnaBI (AGATCT+TACGTA) double digest, 906 thousand fragments (774732+132299 sites), 3286 bp average fragment length
   5% of sequence in fragments shorter than    1155 bp
  10% of sequence in fragments shorter than    1711 bp
  15% of sequence in fragments shorter than    2219 bp
  20% of sequence in fragments shorter than    2699 bp
  25% of sequence in fragments shorter than    3165 bp
  30% of sequence in fragments shorter than    3633 bp
  35% of sequence in fragments shorter than    4107 bp
  40% of sequence in fragments shorter than    4589 bp
  45% of sequence in fragments shorter than    5098 bp
  50% of sequence in fragments shorter than    5635 bp
  55% of sequence in fragments shorter than    6221 bp
  60% of sequence in fragments shorter than    6857 bp
  65% of sequence in fragments shorter than    7570 bp
  70% of sequence in fragments shorter than    8376 bp
  75% of sequence in fragments shorter than    9317 bp
  80% of sequence in fragments shorter than   10481 bp
  85% of sequence in fragments shorter than   12000 bp
  90% of sequence in fragments shorter than   14265 bp
  95% of sequence in fragments shorter than   18715 bp

# ApaI.EcoRV (GGGCCC+GATATC) double digest, 906 thousand fragments (460339+446473 sites), 3287 bp average fragment length
   5% of sequence in fragments shorter than    1108 bp
  10% of sequence in fragments shorter than    1746 bp
  15% of sequence in fragments shorter than    2313 bp
  20% of sequence in fragments shorter than    2853 bp
  25% of sequence in fragments shorter than    3386 bp
  30% of sequence in fragments shorter than    3918 bp
  35% of sequence in fragments shorter than    4459 bp
  40% of sequence in fragments shorter than    5017 bp
  45% of sequence in fragments shorter than    5601 bp
  50% of sequence in fragments shorter than    6232 bp
  55% of sequence in fragments shorter than    6904 bp
  60% of sequence in fragments shorter than    7642 bp
  65% of sequence in fragments shorter than    8458 bp
  70% of sequence in fragments shorter than    9374 bp
  75% of sequence in fragments shorter than   10454 bp
  80% of sequence in fragments shorter than   11791 bp
  85% of sequence in fragments shorter than   13487 bp
  90% of sequence in fragments shorter than   15939 bp
  95% of sequence in fragments shorter than   20575 bp

# AclI.FnuDII (AACGTT+CGCG) double digest, 906 thousand fragments (172706+733940 sites), 3288 bp average fragment length
   5% of sequence in fragments shorter than    1370 bp
  10% of sequence in fragments shorter than    2361 bp
  15% of sequence in fragments shorter than    3248 bp
  20% of sequence in fragments shorter than    4105 bp
  25% of sequence in fragments shorter than    4980 bp
  30% of sequence in fragments shorter than    5889 bp
  35% of sequence in fragments shorter than    6845 bp
  40% of sequence in fragments shorter than    7853 bp
  45% of sequence in fragments shorter than    8928 bp
  50% of sequence in fragments shorter than   10085 bp
  55% of sequence in fragments shorter than   11332 bp
  60% of sequence in fragments shorter than   12723 bp
  65% of sequence in fragments shorter than   14300 bp
  70% of sequence in fragments shorter than   16128 bp
  75% of sequence in fragments shorter than   18275 bp
  80% of sequence in fragments shorter than   20997 bp
  85% of sequence in fragments shorter than   24437 bp
  90% of sequence in fragments shorter than   29367 bp
  95% of sequence in fragments shorter than   38824 bp

# EcoRI.PspXI (GAATTC+VCTCGAGB) double digest, 906 thousand fragments (847401+59193 sites), 3288 bp average fragment length
   5% of sequence in fragments shorter than    1121 bp
  10% of sequence in fragments shorter than    1771 bp
  15% of sequence in fragments shorter than    2324 bp
  20% of sequence in fragments shorter than    2827 bp
  25% of sequence in fragments shorter than    3320 bp
  30% of sequence in fragments shorter than    3802 bp
  35% of sequence in fragments shorter than    4300 bp
  40% of sequence in fragments shorter than    4814 bp
  45% of sequence in fragments shorter than    5350 bp
  50% of sequence in fragments shorter than    5910 bp
  55% of sequence in fragments shorter than    6515 bp
  60% of sequence in fragments shorter than    7181 bp
  65% of sequence in fragments shorter than    7902 bp
  70% of sequence in fragments shorter than    8712 bp
  75% of sequence in fragments shorter than    9658 bp
  80% of sequence in fragments shorter than   10813 bp
  85% of sequence in fragments shorter than   12297 bp
  90% of sequence in fragments shorter than   14365 bp
  95% of sequence in fragments shorter than   17913 bp

# AgeI.XbaI (ACCGGT+TCTAGA) double digest, 903 thousand fragments (53386+850998 sites), 3296 bp average fragment length
   5% of sequence in fragments shorter than    1123 bp
  10% of sequence in fragments shorter than    1694 bp
  15% of sequence in fragments shorter than    2222 bp
  20% of sequence in fragments shorter than    2709 bp
  25% of sequence in fragments shorter than    3201 bp
  30% of sequence in fragments shorter than    3690 bp
  35% of sequence in fragments shorter than    4189 bp
  40% of sequence in fragments shorter than    4700 bp
  45% of sequence in fragments shorter than    5234 bp
  50% of sequence in fragments shorter than    5803 bp
  55% of sequence in fragments shorter than    6411 bp
  60% of sequence in fragments shorter than    7079 bp
  65% of sequence in fragments shorter than    7811 bp
  70% of sequence in fragments shorter than    8634 bp
  75% of sequence in fragments shorter than    9610 bp
  80% of sequence in fragments shorter than   10795 bp
  85% of sequence in fragments shorter than   12324 bp
  90% of sequence in fragments shorter than   14483 bp
  95% of sequence in fragments shorter than   18300 bp

# HindIII.PmeI (AAGCTT+GTTTAAAC) double digest, 902 thousand fragments (860365+42094 sites), 3303 bp average fragment length
   5% of sequence in fragments shorter than    1154 bp
  10% of sequence in fragments shorter than    1758 bp
  15% of sequence in fragments shorter than    2234 bp
  20% of sequence in fragments shorter than    2722 bp
  25% of sequence in fragments shorter than    3191 bp
  30% of sequence in fragments shorter than    3669 bp
  35% of sequence in fragments shorter than    4151 bp
  40% of sequence in fragments shorter than    4647 bp
  45% of sequence in fragments shorter than    5168 bp
  50% of sequence in fragments shorter than    5723 bp
  55% of sequence in fragments shorter than    6327 bp
  60% of sequence in fragments shorter than    6985 bp
  65% of sequence in fragments shorter than    7726 bp
  70% of sequence in fragments shorter than    8562 bp
  75% of sequence in fragments shorter than    9542 bp
  80% of sequence in fragments shorter than   10751 bp
  85% of sequence in fragments shorter than   12340 bp
  90% of sequence in fragments shorter than   14684 bp
  95% of sequence in fragments shorter than   19335 bp

# SacI.SexAI (GAGCTC+ACCWGGT) double digest, 900 thousand fragments (603323+297900 sites), 3308 bp average fragment length
   5% of sequence in fragments shorter than    1132 bp
  10% of sequence in fragments shorter than    1729 bp
  15% of sequence in fragments shorter than    2249 bp
  20% of sequence in fragments shorter than    2756 bp
  25% of sequence in fragments shorter than    3265 bp
  30% of sequence in fragments shorter than    3776 bp
  35% of sequence in fragments shorter than    4301 bp
  40% of sequence in fragments shorter than    4842 bp
  45% of sequence in fragments shorter than    5404 bp
  50% of sequence in fragments shorter than    6011 bp
  55% of sequence in fragments shorter than    6665 bp
  60% of sequence in fragments shorter than    7378 bp
  65% of sequence in fragments shorter than    8185 bp
  70% of sequence in fragments shorter than    9095 bp
  75% of sequence in fragments shorter than   10176 bp
  80% of sequence in fragments shorter than   11508 bp
  85% of sequence in fragments shorter than   13228 bp
  90% of sequence in fragments shorter than   15771 bp
  95% of sequence in fragments shorter than   20681 bp

# AgeI.EcoRI (ACCGGT+GAATTC) double digest, 900 thousand fragments (53386+847401 sites), 3309 bp average fragment length
   5% of sequence in fragments shorter than    1132 bp
  10% of sequence in fragments shorter than    1771 bp
  15% of sequence in fragments shorter than    2327 bp
  20% of sequence in fragments shorter than    2836 bp
  25% of sequence in fragments shorter than    3336 bp
  30% of sequence in fragments shorter than    3826 bp
  35% of sequence in fragments shorter than    4330 bp
  40% of sequence in fragments shorter than    4851 bp
  45% of sequence in fragments shorter than    5398 bp
  50% of sequence in fragments shorter than    5966 bp
  55% of sequence in fragments shorter than    6579 bp
  60% of sequence in fragments shorter than    7255 bp
  65% of sequence in fragments shorter than    7992 bp
  70% of sequence in fragments shorter than    8825 bp
  75% of sequence in fragments shorter than    9799 bp
  80% of sequence in fragments shorter than   10979 bp
  85% of sequence in fragments shorter than   12476 bp
  90% of sequence in fragments shorter than   14609 bp
  95% of sequence in fragments shorter than   18354 bp

# BglII.NaeI (AGATCT+GCCGGC) double digest, 900 thousand fragments (774732+125692 sites), 3310 bp average fragment length
   5% of sequence in fragments shorter than    1160 bp
  10% of sequence in fragments shorter than    1742 bp
  15% of sequence in fragments shorter than    2264 bp
  20% of sequence in fragments shorter than    2756 bp
  25% of sequence in fragments shorter than    3241 bp
  30% of sequence in fragments shorter than    3716 bp
  35% of sequence in fragments shorter than    4202 bp
  40% of sequence in fragments shorter than    4695 bp
  45% of sequence in fragments shorter than    5206 bp
  50% of sequence in fragments shorter than    5754 bp
  55% of sequence in fragments shorter than    6340 bp
  60% of sequence in fragments shorter than    6982 bp
  65% of sequence in fragments shorter than    7700 bp
  70% of sequence in fragments shorter than    8502 bp
  75% of sequence in fragments shorter than    9456 bp
  80% of sequence in fragments shorter than   10607 bp
  85% of sequence in fragments shorter than   12096 bp
  90% of sequence in fragments shorter than   14289 bp
  95% of sequence in fragments shorter than   18667 bp

# BstEII.MfeI (GGTNACC+CAATTG) double digest, 899 thousand fragments (335865+564303 sites), 3312 bp average fragment length
   5% of sequence in fragments shorter than    1166 bp
  10% of sequence in fragments shorter than    1773 bp
  15% of sequence in fragments shorter than    2269 bp
  20% of sequence in fragments shorter than    2702 bp
  25% of sequence in fragments shorter than    3162 bp
  30% of sequence in fragments shorter than    3624 bp
  35% of sequence in fragments shorter than    4092 bp
  40% of sequence in fragments shorter than    4571 bp
  45% of sequence in fragments shorter than    5074 bp
  50% of sequence in fragments shorter than    5613 bp
  55% of sequence in fragments shorter than    6196 bp
  60% of sequence in fragments shorter than    6825 bp
  65% of sequence in fragments shorter than    7522 bp
  70% of sequence in fragments shorter than    8329 bp
  75% of sequence in fragments shorter than    9259 bp
  80% of sequence in fragments shorter than   10398 bp
  85% of sequence in fragments shorter than   11843 bp
  90% of sequence in fragments shorter than   13946 bp
  95% of sequence in fragments shorter than   17950 bp

# BclI.PacI (TGATCA+TTAATTAA) double digest, 897 thousand fragments (738785+159455 sites), 3318 bp average fragment length
   5% of sequence in fragments shorter than    1175 bp
  10% of sequence in fragments shorter than    1765 bp
  15% of sequence in fragments shorter than    2286 bp
  20% of sequence in fragments shorter than    2758 bp
  25% of sequence in fragments shorter than    3231 bp
  30% of sequence in fragments shorter than    3711 bp
  35% of sequence in fragments shorter than    4188 bp
  40% of sequence in fragments shorter than    4683 bp
  45% of sequence in fragments shorter than    5198 bp
  50% of sequence in fragments shorter than    5746 bp
  55% of sequence in fragments shorter than    6339 bp
  60% of sequence in fragments shorter than    6992 bp
  65% of sequence in fragments shorter than    7714 bp
  70% of sequence in fragments shorter than    8547 bp
  75% of sequence in fragments shorter than    9536 bp
  80% of sequence in fragments shorter than   10750 bp
  85% of sequence in fragments shorter than   12342 bp
  90% of sequence in fragments shorter than   14738 bp
  95% of sequence in fragments shorter than   20060 bp

# BclI.Hpy99I (TGATCA+CGWCG) double digest, 896 thousand fragments (738785+157917 sites), 3324 bp average fragment length
   5% of sequence in fragments shorter than    1181 bp
  10% of sequence in fragments shorter than    1789 bp
  15% of sequence in fragments shorter than    2319 bp
  20% of sequence in fragments shorter than    2793 bp
  25% of sequence in fragments shorter than    3271 bp
  30% of sequence in fragments shorter than    3737 bp
  35% of sequence in fragments shorter than    4216 bp
  40% of sequence in fragments shorter than    4711 bp
  45% of sequence in fragments shorter than    5228 bp
  50% of sequence in fragments shorter than    5777 bp
  55% of sequence in fragments shorter than    6369 bp
  60% of sequence in fragments shorter than    7005 bp
  65% of sequence in fragments shorter than    7709 bp
  70% of sequence in fragments shorter than    8514 bp
  75% of sequence in fragments shorter than    9440 bp
  80% of sequence in fragments shorter than   10587 bp
  85% of sequence in fragments shorter than   12052 bp
  90% of sequence in fragments shorter than   14180 bp
  95% of sequence in fragments shorter than   18397 bp

# ApaLI.SpeI (GTGCAC+ACTAGT) double digest, 896 thousand fragments (496255+400286 sites), 3325 bp average fragment length
   5% of sequence in fragments shorter than    1165 bp
  10% of sequence in fragments shorter than    1781 bp
  15% of sequence in fragments shorter than    2303 bp
  20% of sequence in fragments shorter than    2792 bp
  25% of sequence in fragments shorter than    3273 bp
  30% of sequence in fragments shorter than    3751 bp
  35% of sequence in fragments shorter than    4239 bp
  40% of sequence in fragments shorter than    4736 bp
  45% of sequence in fragments shorter than    5252 bp
  50% of sequence in fragments shorter than    5780 bp
  55% of sequence in fragments shorter than    6360 bp
  60% of sequence in fragments shorter than    6993 bp
  65% of sequence in fragments shorter than    7689 bp
  70% of sequence in fragments shorter than    8484 bp
  75% of sequence in fragments shorter than    9395 bp
  80% of sequence in fragments shorter than   10503 bp
  85% of sequence in fragments shorter than   11922 bp
  90% of sequence in fragments shorter than   13995 bp
  95% of sequence in fragments shorter than   17910 bp

# BglII.XhoI (AGATCT+CTCGAG) double digest, 895 thousand fragments (774732+121308 sites), 3327 bp average fragment length
   5% of sequence in fragments shorter than    1147 bp
  10% of sequence in fragments shorter than    1744 bp
  15% of sequence in fragments shorter than    2261 bp
  20% of sequence in fragments shorter than    2751 bp
  25% of sequence in fragments shorter than    3232 bp
  30% of sequence in fragments shorter than    3703 bp
  35% of sequence in fragments shorter than    4183 bp
  40% of sequence in fragments shorter than    4669 bp
  45% of sequence in fragments shorter than    5175 bp
  50% of sequence in fragments shorter than    5710 bp
  55% of sequence in fragments shorter than    6291 bp
  60% of sequence in fragments shorter than    6922 bp
  65% of sequence in fragments shorter than    7632 bp
  70% of sequence in fragments shorter than    8426 bp
  75% of sequence in fragments shorter than    9343 bp
  80% of sequence in fragments shorter than   10488 bp
  85% of sequence in fragments shorter than   11941 bp
  90% of sequence in fragments shorter than   14094 bp
  95% of sequence in fragments shorter than   18360 bp

# FnuDII.PacI (CGCG+TTAATTAA) double digest, 893 thousand fragments (733940+159455 sites), 3337 bp average fragment length
   5% of sequence in fragments shorter than    1462 bp
  10% of sequence in fragments shorter than    2487 bp
  15% of sequence in fragments shorter than    3393 bp
  20% of sequence in fragments shorter than    4280 bp
  25% of sequence in fragments shorter than    5176 bp
  30% of sequence in fragments shorter than    6101 bp
  35% of sequence in fragments shorter than    7047 bp
  40% of sequence in fragments shorter than    8056 bp
  45% of sequence in fragments shorter than    9122 bp
  50% of sequence in fragments shorter than   10275 bp
  55% of sequence in fragments shorter than   11516 bp
  60% of sequence in fragments shorter than   12910 bp
  65% of sequence in fragments shorter than   14497 bp
  70% of sequence in fragments shorter than   16329 bp
  75% of sequence in fragments shorter than   18461 bp
  80% of sequence in fragments shorter than   21131 bp
  85% of sequence in fragments shorter than   24584 bp
  90% of sequence in fragments shorter than   29664 bp
  95% of sequence in fragments shorter than   39956 bp

# PmeI.XbaI (GTTTAAAC+TCTAGA) double digest, 892 thousand fragments (42094+850998 sites), 3338 bp average fragment length
   5% of sequence in fragments shorter than    1129 bp
  10% of sequence in fragments shorter than    1718 bp
  15% of sequence in fragments shorter than    2242 bp
  20% of sequence in fragments shorter than    2751 bp
  25% of sequence in fragments shorter than    3249 bp
  30% of sequence in fragments shorter than    3747 bp
  35% of sequence in fragments shorter than    4257 bp
  40% of sequence in fragments shorter than    4779 bp
  45% of sequence in fragments shorter than    5320 bp
  50% of sequence in fragments shorter than    5897 bp
  55% of sequence in fragments shorter than    6522 bp
  60% of sequence in fragments shorter than    7207 bp
  65% of sequence in fragments shorter than    7958 bp
  70% of sequence in fragments shorter than    8823 bp
  75% of sequence in fragments shorter than    9833 bp
  80% of sequence in fragments shorter than   11089 bp
  85% of sequence in fragments shorter than   12697 bp
  90% of sequence in fragments shorter than   15021 bp
  95% of sequence in fragments shorter than   19300 bp

# AvrII.SexAI (CCTAGG+ACCWGGT) double digest, 892 thousand fragments (594956+297900 sites), 3338 bp average fragment length
   5% of sequence in fragments shorter than    1147 bp
  10% of sequence in fragments shorter than    1761 bp
  15% of sequence in fragments shorter than    2295 bp
  20% of sequence in fragments shorter than    2795 bp
  25% of sequence in fragments shorter than    3286 bp
  30% of sequence in fragments shorter than    3772 bp
  35% of sequence in fragments shorter than    4265 bp
  40% of sequence in fragments shorter than    4758 bp
  45% of sequence in fragments shorter than    5287 bp
  50% of sequence in fragments shorter than    5845 bp
  55% of sequence in fragments shorter than    6456 bp
  60% of sequence in fragments shorter than    7130 bp
  65% of sequence in fragments shorter than    7878 bp
  70% of sequence in fragments shorter than    8725 bp
  75% of sequence in fragments shorter than    9728 bp
  80% of sequence in fragments shorter than   10949 bp
  85% of sequence in fragments shorter than   12543 bp
  90% of sequence in fragments shorter than   14913 bp
  95% of sequence in fragments shorter than   19681 bp

# NcoI.SnaBI (CCATGG+TACGTA) double digest, 891 thousand fragments (759594+132299 sites), 3342 bp average fragment length
   5% of sequence in fragments shorter than    1164 bp
  10% of sequence in fragments shorter than    1788 bp
  15% of sequence in fragments shorter than    2328 bp
  20% of sequence in fragments shorter than    2833 bp
  25% of sequence in fragments shorter than    3329 bp
  30% of sequence in fragments shorter than    3827 bp
  35% of sequence in fragments shorter than    4333 bp
  40% of sequence in fragments shorter than    4832 bp
  45% of sequence in fragments shorter than    5380 bp
  50% of sequence in fragments shorter than    5939 bp
  55% of sequence in fragments shorter than    6547 bp
  60% of sequence in fragments shorter than    7231 bp
  65% of sequence in fragments shorter than    7997 bp
  70% of sequence in fragments shorter than    8870 bp
  75% of sequence in fragments shorter than    9861 bp
  80% of sequence in fragments shorter than   11079 bp
  85% of sequence in fragments shorter than   12686 bp
  90% of sequence in fragments shorter than   14973 bp
  95% of sequence in fragments shorter than   19524 bp

# FnuDII.Hpy99I (CGCG+CGWCG) double digest, 891 thousand fragments (733940+157917 sites), 3342 bp average fragment length
   5% of sequence in fragments shorter than    1463 bp
  10% of sequence in fragments shorter than    2623 bp
  15% of sequence in fragments shorter than    3718 bp
  20% of sequence in fragments shorter than    4846 bp
  25% of sequence in fragments shorter than    6013 bp
  30% of sequence in fragments shorter than    7233 bp
  35% of sequence in fragments shorter than    8573 bp
  40% of sequence in fragments shorter than    9997 bp
  45% of sequence in fragments shorter than   11533 bp
  50% of sequence in fragments shorter than   13252 bp
  55% of sequence in fragments shorter than   15177 bp
  60% of sequence in fragments shorter than   17345 bp
  65% of sequence in fragments shorter than   19805 bp
  70% of sequence in fragments shorter than   22721 bp
  75% of sequence in fragments shorter than   26106 bp
  80% of sequence in fragments shorter than   30427 bp
  85% of sequence in fragments shorter than   36014 bp
  90% of sequence in fragments shorter than   44407 bp
  95% of sequence in fragments shorter than   60473 bp

# HindIII.SalI (AAGCTT+GTCGAC) double digest, 891 thousand fragments (860365+31396 sites), 3343 bp average fragment length
   5% of sequence in fragments shorter than    1170 bp
  10% of sequence in fragments shorter than    1783 bp
  15% of sequence in fragments shorter than    2266 bp
  20% of sequence in fragments shorter than    2759 bp
  25% of sequence in fragments shorter than    3234 bp
  30% of sequence in fragments shorter than    3719 bp
  35% of sequence in fragments shorter than    4205 bp
  40% of sequence in fragments shorter than    4705 bp
  45% of sequence in fragments shorter than    5231 bp
  50% of sequence in fragments shorter than    5790 bp
  55% of sequence in fragments shorter than    6397 bp
  60% of sequence in fragments shorter than    7054 bp
  65% of sequence in fragments shorter than    7798 bp
  70% of sequence in fragments shorter than    8632 bp
  75% of sequence in fragments shorter than    9611 bp
  80% of sequence in fragments shorter than   10806 bp
  85% of sequence in fragments shorter than   12361 bp
  90% of sequence in fragments shorter than   14649 bp
  95% of sequence in fragments shorter than   19083 bp

# KpnI.SacI (GGTACC+GAGCTC) double digest, 890 thousand fragments (287974+603323 sites), 3344 bp average fragment length
   5% of sequence in fragments shorter than    1177 bp
  10% of sequence in fragments shorter than    1709 bp
  15% of sequence in fragments shorter than    2233 bp
  20% of sequence in fragments shorter than    2755 bp
  25% of sequence in fragments shorter than    3259 bp
  30% of sequence in fragments shorter than    3775 bp
  35% of sequence in fragments shorter than    4299 bp
  40% of sequence in fragments shorter than    4845 bp
  45% of sequence in fragments shorter than    5418 bp
  50% of sequence in fragments shorter than    6031 bp
  55% of sequence in fragments shorter than    6688 bp
  60% of sequence in fragments shorter than    7412 bp
  65% of sequence in fragments shorter than    8205 bp
  70% of sequence in fragments shorter than    9119 bp
  75% of sequence in fragments shorter than   10215 bp
  80% of sequence in fragments shorter than   11522 bp
  85% of sequence in fragments shorter than   13256 bp
  90% of sequence in fragments shorter than   15771 bp
  95% of sequence in fragments shorter than   20844 bp

# NheI.SacI (GCTAGC+GAGCTC) double digest, 890 thousand fragments (287384+603323 sites), 3347 bp average fragment length
   5% of sequence in fragments shorter than    1158 bp
  10% of sequence in fragments shorter than    1718 bp
  15% of sequence in fragments shorter than    2231 bp
  20% of sequence in fragments shorter than    2750 bp
  25% of sequence in fragments shorter than    3258 bp
  30% of sequence in fragments shorter than    3773 bp
  35% of sequence in fragments shorter than    4298 bp
  40% of sequence in fragments shorter than    4839 bp
  45% of sequence in fragments shorter than    5405 bp
  50% of sequence in fragments shorter than    6010 bp
  55% of sequence in fragments shorter than    6658 bp
  60% of sequence in fragments shorter than    7365 bp
  65% of sequence in fragments shorter than    8163 bp
  70% of sequence in fragments shorter than    9068 bp
  75% of sequence in fragments shorter than   10129 bp
  80% of sequence in fragments shorter than   11412 bp
  85% of sequence in fragments shorter than   13085 bp
  90% of sequence in fragments shorter than   15490 bp
  95% of sequence in fragments shorter than   20188 bp

# EcoRI.PmeI (GAATTC+GTTTAAAC) double digest, 889 thousand fragments (847401+42094 sites), 3351 bp average fragment length
   5% of sequence in fragments shorter than    1138 bp
  10% of sequence in fragments shorter than    1803 bp
  15% of sequence in fragments shorter than    2370 bp
  20% of sequence in fragments shorter than    2886 bp
  25% of sequence in fragments shorter than    3385 bp
  30% of sequence in fragments shorter than    3889 bp
  35% of sequence in fragments shorter than    4399 bp
  40% of sequence in fragments shorter than    4933 bp
  45% of sequence in fragments shorter than    5486 bp
  50% of sequence in fragments shorter than    6067 bp
  55% of sequence in fragments shorter than    6701 bp
  60% of sequence in fragments shorter than    7394 bp
  65% of sequence in fragments shorter than    8155 bp
  70% of sequence in fragments shorter than    9009 bp
  75% of sequence in fragments shorter than   10021 bp
  80% of sequence in fragments shorter than   11258 bp
  85% of sequence in fragments shorter than   12839 bp
  90% of sequence in fragments shorter than   15141 bp
  95% of sequence in fragments shorter than   19308 bp

# BstEII.SphI (GGTNACC+GCATGC) double digest, 886 thousand fragments (335865+550952 sites), 3361 bp average fragment length
   5% of sequence in fragments shorter than    1164 bp
  10% of sequence in fragments shorter than    1769 bp
  15% of sequence in fragments shorter than    2293 bp
  20% of sequence in fragments shorter than    2795 bp
  25% of sequence in fragments shorter than    3290 bp
  30% of sequence in fragments shorter than    3782 bp
  35% of sequence in fragments shorter than    4287 bp
  40% of sequence in fragments shorter than    4812 bp
  45% of sequence in fragments shorter than    5354 bp
  50% of sequence in fragments shorter than    5927 bp
  55% of sequence in fragments shorter than    6541 bp
  60% of sequence in fragments shorter than    7226 bp
  65% of sequence in fragments shorter than    7983 bp
  70% of sequence in fragments shorter than    8848 bp
  75% of sequence in fragments shorter than    9881 bp
  80% of sequence in fragments shorter than   11132 bp
  85% of sequence in fragments shorter than   12781 bp
  90% of sequence in fragments shorter than   15162 bp
  95% of sequence in fragments shorter than   19956 bp

# AclI.BsaAI (AACGTT+YACGTR) double digest, 886 thousand fragments (172706+713801 sites), 3363 bp average fragment length
   5% of sequence in fragments shorter than    1179 bp
  10% of sequence in fragments shorter than    1810 bp
  15% of sequence in fragments shorter than    2351 bp
  20% of sequence in fragments shorter than    2854 bp
  25% of sequence in fragments shorter than    3358 bp
  30% of sequence in fragments shorter than    3841 bp
  35% of sequence in fragments shorter than    4352 bp
  40% of sequence in fragments shorter than    4876 bp
  45% of sequence in fragments shorter than    5429 bp
  50% of sequence in fragments shorter than    6018 bp
  55% of sequence in fragments shorter than    6651 bp
  60% of sequence in fragments shorter than    7333 bp
  65% of sequence in fragments shorter than    8098 bp
  70% of sequence in fragments shorter than    8958 bp
  75% of sequence in fragments shorter than    9963 bp
  80% of sequence in fragments shorter than   11161 bp
  85% of sequence in fragments shorter than   12707 bp
  90% of sequence in fragments shorter than   14940 bp
  95% of sequence in fragments shorter than   18799 bp

# ApaLI.HpaI (GTGCAC+GTTAAC) double digest, 885 thousand fragments (496255+389418 sites), 3366 bp average fragment length
   5% of sequence in fragments shorter than    1202 bp
  10% of sequence in fragments shorter than    1806 bp
  15% of sequence in fragments shorter than    2329 bp
  20% of sequence in fragments shorter than    2811 bp
  25% of sequence in fragments shorter than    3284 bp
  30% of sequence in fragments shorter than    3719 bp
  35% of sequence in fragments shorter than    4202 bp
  40% of sequence in fragments shorter than    4697 bp
  45% of sequence in fragments shorter than    5215 bp
  50% of sequence in fragments shorter than    5756 bp
  55% of sequence in fragments shorter than    6333 bp
  60% of sequence in fragments shorter than    6971 bp
  65% of sequence in fragments shorter than    7678 bp
  70% of sequence in fragments shorter than    8476 bp
  75% of sequence in fragments shorter than    9406 bp
  80% of sequence in fragments shorter than   10529 bp
  85% of sequence in fragments shorter than   11994 bp
  90% of sequence in fragments shorter than   14127 bp
  95% of sequence in fragments shorter than   18197 bp

# NaeI.NcoI (GCCGGC+CCATGG) double digest, 884 thousand fragments (125692+759594 sites), 3367 bp average fragment length
   5% of sequence in fragments shorter than    1157 bp
  10% of sequence in fragments shorter than    1799 bp
  15% of sequence in fragments shorter than    2373 bp
  20% of sequence in fragments shorter than    2924 bp
  25% of sequence in fragments shorter than    3467 bp
  30% of sequence in fragments shorter than    4017 bp
  35% of sequence in fragments shorter than    4556 bp
  40% of sequence in fragments shorter than    5131 bp
  45% of sequence in fragments shorter than    5720 bp
  50% of sequence in fragments shorter than    6356 bp
  55% of sequence in fragments shorter than    7055 bp
  60% of sequence in fragments shorter than    7822 bp
  65% of sequence in fragments shorter than    8688 bp
  70% of sequence in fragments shorter than    9672 bp
  75% of sequence in fragments shorter than   10831 bp
  80% of sequence in fragments shorter than   12259 bp
  85% of sequence in fragments shorter than   14122 bp
  90% of sequence in fragments shorter than   16849 bp
  95% of sequence in fragments shorter than   22366 bp

# AvrII.KpnI (CCTAGG+GGTACC) double digest, 882 thousand fragments (594956+287974 sites), 3376 bp average fragment length
   5% of sequence in fragments shorter than    1163 bp
  10% of sequence in fragments shorter than    1782 bp
  15% of sequence in fragments shorter than    2327 bp
  20% of sequence in fragments shorter than    2845 bp
  25% of sequence in fragments shorter than    3354 bp
  30% of sequence in fragments shorter than    3852 bp
  35% of sequence in fragments shorter than    4365 bp
  40% of sequence in fragments shorter than    4903 bp
  45% of sequence in fragments shorter than    5474 bp
  50% of sequence in fragments shorter than    6076 bp
  55% of sequence in fragments shorter than    6724 bp
  60% of sequence in fragments shorter than    7433 bp
  65% of sequence in fragments shorter than    8216 bp
  70% of sequence in fragments shorter than    9113 bp
  75% of sequence in fragments shorter than   10162 bp
  80% of sequence in fragments shorter than   11446 bp
  85% of sequence in fragments shorter than   13143 bp
  90% of sequence in fragments shorter than   15660 bp
  95% of sequence in fragments shorter than   20844 bp

# AvrII.NheI (CCTAGG+GCTAGC) double digest, 881 thousand fragments (594956+287384 sites), 3378 bp average fragment length
   5% of sequence in fragments shorter than    1192 bp
  10% of sequence in fragments shorter than    1808 bp
  15% of sequence in fragments shorter than    2357 bp
  20% of sequence in fragments shorter than    2846 bp
  25% of sequence in fragments shorter than    3359 bp
  30% of sequence in fragments shorter than    3874 bp
  35% of sequence in fragments shorter than    4392 bp
  40% of sequence in fragments shorter than    4929 bp
  45% of sequence in fragments shorter than    5494 bp
  50% of sequence in fragments shorter than    6087 bp
  55% of sequence in fragments shorter than    6731 bp
  60% of sequence in fragments shorter than    7436 bp
  65% of sequence in fragments shorter than    8209 bp
  70% of sequence in fragments shorter than    9090 bp
  75% of sequence in fragments shorter than   10132 bp
  80% of sequence in fragments shorter than   11380 bp
  85% of sequence in fragments shorter than   13030 bp
  90% of sequence in fragments shorter than   15429 bp
  95% of sequence in fragments shorter than   20147 bp

# SalI.XbaI (GTCGAC+TCTAGA) double digest, 881 thousand fragments (31396+850998 sites), 3378 bp average fragment length
   5% of sequence in fragments shorter than    1148 bp
  10% of sequence in fragments shorter than    1738 bp
  15% of sequence in fragments shorter than    2266 bp
  20% of sequence in fragments shorter than    2790 bp
  25% of sequence in fragments shorter than    3295 bp
  30% of sequence in fragments shorter than    3796 bp
  35% of sequence in fragments shorter than    4311 bp
  40% of sequence in fragments shorter than    4837 bp
  45% of sequence in fragments shorter than    5384 bp
  50% of sequence in fragments shorter than    5966 bp
  55% of sequence in fragments shorter than    6595 bp
  60% of sequence in fragments shorter than    7278 bp
  65% of sequence in fragments shorter than    8031 bp
  70% of sequence in fragments shorter than    8892 bp
  75% of sequence in fragments shorter than    9901 bp
  80% of sequence in fragments shorter than   11144 bp
  85% of sequence in fragments shorter than   12720 bp
  90% of sequence in fragments shorter than   14983 bp
  95% of sequence in fragments shorter than   19060 bp

# HindIII.MluI (AAGCTT+ACGCGT) double digest, 881 thousand fragments (860365+21415 sites), 3381 bp average fragment length
   5% of sequence in fragments shorter than    1187 bp
  10% of sequence in fragments shorter than    1805 bp
  15% of sequence in fragments shorter than    2291 bp
  20% of sequence in fragments shorter than    2788 bp
  25% of sequence in fragments shorter than    3271 bp
  30% of sequence in fragments shorter than    3759 bp
  35% of sequence in fragments shorter than    4251 bp
  40% of sequence in fragments shorter than    4758 bp
  45% of sequence in fragments shorter than    5284 bp
  50% of sequence in fragments shorter than    5848 bp
  55% of sequence in fragments shorter than    6459 bp
  60% of sequence in fragments shorter than    7118 bp
  65% of sequence in fragments shorter than    7869 bp
  70% of sequence in fragments shorter than    8712 bp
  75% of sequence in fragments shorter than    9689 bp
  80% of sequence in fragments shorter than   10890 bp
  85% of sequence in fragments shorter than   12440 bp
  90% of sequence in fragments shorter than   14719 bp
  95% of sequence in fragments shorter than   19125 bp

# NcoI.XhoI (CCATGG+CTCGAG) double digest, 880 thousand fragments (759594+121308 sites), 3384 bp average fragment length
   5% of sequence in fragments shorter than    1169 bp
  10% of sequence in fragments shorter than    1788 bp
  15% of sequence in fragments shorter than    2340 bp
  20% of sequence in fragments shorter than    2868 bp
  25% of sequence in fragments shorter than    3383 bp
  30% of sequence in fragments shorter than    3903 bp
  35% of sequence in fragments shorter than    4376 bp
  40% of sequence in fragments shorter than    4899 bp
  45% of sequence in fragments shorter than    5468 bp
  50% of sequence in fragments shorter than    6064 bp
  55% of sequence in fragments shorter than    6721 bp
  60% of sequence in fragments shorter than    7449 bp
  65% of sequence in fragments shorter than    8270 bp
  70% of sequence in fragments shorter than    9214 bp
  75% of sequence in fragments shorter than   10313 bp
  80% of sequence in fragments shorter than   11667 bp
  85% of sequence in fragments shorter than   13413 bp
  90% of sequence in fragments shorter than   15973 bp
  95% of sequence in fragments shorter than   21165 bp

# BstEII.ScaI (GGTNACC+AGTACT) double digest, 879 thousand fragments (335865+543793 sites), 3389 bp average fragment length
   5% of sequence in fragments shorter than    1195 bp
  10% of sequence in fragments shorter than    1800 bp
  15% of sequence in fragments shorter than    2320 bp
  20% of sequence in fragments shorter than    2805 bp
  25% of sequence in fragments shorter than    3283 bp
  30% of sequence in fragments shorter than    3764 bp
  35% of sequence in fragments shorter than    4251 bp
  40% of sequence in fragments shorter than    4747 bp
  45% of sequence in fragments shorter than    5268 bp
  50% of sequence in fragments shorter than    5816 bp
  55% of sequence in fragments shorter than    6404 bp
  60% of sequence in fragments shorter than    7036 bp
  65% of sequence in fragments shorter than    7745 bp
  70% of sequence in fragments shorter than    8542 bp
  75% of sequence in fragments shorter than    9473 bp
  80% of sequence in fragments shorter than   10625 bp
  85% of sequence in fragments shorter than   12137 bp
  90% of sequence in fragments shorter than   14340 bp
  95% of sequence in fragments shorter than   18655 bp

# AflII.BsrBI (CTTAAG+CCGCTC) double digest, 878 thousand fragments (643284+235523 sites), 3392 bp average fragment length
   5% of sequence in fragments shorter than    1201 bp
  10% of sequence in fragments shorter than    1816 bp
  15% of sequence in fragments shorter than    2352 bp
  20% of sequence in fragments shorter than    2858 bp
  25% of sequence in fragments shorter than    3355 bp
  30% of sequence in fragments shorter than    3845 bp
  35% of sequence in fragments shorter than    4345 bp
  40% of sequence in fragments shorter than    4858 bp
  45% of sequence in fragments shorter than    5389 bp
  50% of sequence in fragments shorter than    5952 bp
  55% of sequence in fragments shorter than    6561 bp
  60% of sequence in fragments shorter than    7223 bp
  65% of sequence in fragments shorter than    7960 bp
  70% of sequence in fragments shorter than    8788 bp
  75% of sequence in fragments shorter than    9745 bp
  80% of sequence in fragments shorter than   10910 bp
  85% of sequence in fragments shorter than   12413 bp
  90% of sequence in fragments shorter than   14601 bp
  95% of sequence in fragments shorter than   18674 bp

# EcoRI.SalI (GAATTC+GTCGAC) double digest, 878 thousand fragments (847401+31396 sites), 3392 bp average fragment length
   5% of sequence in fragments shorter than    1157 bp
  10% of sequence in fragments shorter than    1830 bp
  15% of sequence in fragments shorter than    2402 bp
  20% of sequence in fragments shorter than    2922 bp
  25% of sequence in fragments shorter than    3423 bp
  30% of sequence in fragments shorter than    3934 bp
  35% of sequence in fragments shorter than    4449 bp
  40% of sequence in fragments shorter than    4988 bp
  45% of sequence in fragments shorter than    5547 bp
  50% of sequence in fragments shorter than    6131 bp
  55% of sequence in fragments shorter than    6765 bp
  60% of sequence in fragments shorter than    7459 bp
  65% of sequence in fragments shorter than    8222 bp
  70% of sequence in fragments shorter than    9080 bp
  75% of sequence in fragments shorter than   10093 bp
  80% of sequence in fragments shorter than   11309 bp
  85% of sequence in fragments shorter than   12869 bp
  90% of sequence in fragments shorter than   15083 bp
  95% of sequence in fragments shorter than   19100 bp

# AclI.BceAI (AACGTT+ACGGC) double digest, 877 thousand fragments (172706+705178 sites), 3396 bp average fragment length
   5% of sequence in fragments shorter than    1115 bp
  10% of sequence in fragments shorter than    1805 bp
  15% of sequence in fragments shorter than    2425 bp
  20% of sequence in fragments shorter than    3033 bp
  25% of sequence in fragments shorter than    3623 bp
  30% of sequence in fragments shorter than    4230 bp
  35% of sequence in fragments shorter than    4859 bp
  40% of sequence in fragments shorter than    5519 bp
  45% of sequence in fragments shorter than    6214 bp
  50% of sequence in fragments shorter than    6955 bp
  55% of sequence in fragments shorter than    7773 bp
  60% of sequence in fragments shorter than    8666 bp
  65% of sequence in fragments shorter than    9658 bp
  70% of sequence in fragments shorter than   10799 bp
  75% of sequence in fragments shorter than   12126 bp
  80% of sequence in fragments shorter than   13749 bp
  85% of sequence in fragments shorter than   15820 bp
  90% of sequence in fragments shorter than   18825 bp
  95% of sequence in fragments shorter than   24306 bp

# HindIII.SgrAI (AAGCTT+CRCCGGYG) double digest, 875 thousand fragments (860365+15270 sites), 3404 bp average fragment length
   5% of sequence in fragments shorter than    1193 bp
  10% of sequence in fragments shorter than    1815 bp
  15% of sequence in fragments shorter than    2306 bp
  20% of sequence in fragments shorter than    2807 bp
  25% of sequence in fragments shorter than    3294 bp
  30% of sequence in fragments shorter than    3786 bp
  35% of sequence in fragments shorter than    4283 bp
  40% of sequence in fragments shorter than    4792 bp
  45% of sequence in fragments shorter than    5326 bp
  50% of sequence in fragments shorter than    5894 bp
  55% of sequence in fragments shorter than    6511 bp
  60% of sequence in fragments shorter than    7180 bp
  65% of sequence in fragments shorter than    7935 bp
  70% of sequence in fragments shorter than    8780 bp
  75% of sequence in fragments shorter than    9779 bp
  80% of sequence in fragments shorter than   10983 bp
  85% of sequence in fragments shorter than   12552 bp
  90% of sequence in fragments shorter than   14852 bp
  95% of sequence in fragments shorter than   19340 bp

# HindIII.NruI (AAGCTT+TCGCGA) double digest, 874 thousand fragments (860365+14872 sites), 3406 bp average fragment length
   5% of sequence in fragments shorter than    1193 bp
  10% of sequence in fragments shorter than    1815 bp
  15% of sequence in fragments shorter than    2305 bp
  20% of sequence in fragments shorter than    2807 bp
  25% of sequence in fragments shorter than    3294 bp
  30% of sequence in fragments shorter than    3786 bp
  35% of sequence in fragments shorter than    4281 bp
  40% of sequence in fragments shorter than    4791 bp
  45% of sequence in fragments shorter than    5323 bp
  50% of sequence in fragments shorter than    5891 bp
  55% of sequence in fragments shorter than    6511 bp
  60% of sequence in fragments shorter than    7184 bp
  65% of sequence in fragments shorter than    7942 bp
  70% of sequence in fragments shorter than    8788 bp
  75% of sequence in fragments shorter than    9793 bp
  80% of sequence in fragments shorter than   11004 bp
  85% of sequence in fragments shorter than   12589 bp
  90% of sequence in fragments shorter than   14909 bp
  95% of sequence in fragments shorter than   19483 bp

# HindIII.PvuI (AAGCTT+CGATCG) double digest, 874 thousand fragments (860365+14186 sites), 3409 bp average fragment length
   5% of sequence in fragments shorter than    1196 bp
  10% of sequence in fragments shorter than    1817 bp
  15% of sequence in fragments shorter than    2304 bp
  20% of sequence in fragments shorter than    2806 bp
  25% of sequence in fragments shorter than    3295 bp
  30% of sequence in fragments shorter than    3788 bp
  35% of sequence in fragments shorter than    4287 bp
  40% of sequence in fragments shorter than    4798 bp
  45% of sequence in fragments shorter than    5333 bp
  50% of sequence in fragments shorter than    5904 bp
  55% of sequence in fragments shorter than    6522 bp
  60% of sequence in fragments shorter than    7195 bp
  65% of sequence in fragments shorter than    7957 bp
  70% of sequence in fragments shorter than    8808 bp
  75% of sequence in fragments shorter than    9813 bp
  80% of sequence in fragments shorter than   11029 bp
  85% of sequence in fragments shorter than   12621 bp
  90% of sequence in fragments shorter than   14931 bp
  95% of sequence in fragments shorter than   19425 bp

# FseI.HindIII (GGCCGGCC+AAGCTT) double digest, 873 thousand fragments (13662+860365 sites), 3411 bp average fragment length
   5% of sequence in fragments shorter than    1196 bp
  10% of sequence in fragments shorter than    1820 bp
  15% of sequence in fragments shorter than    2311 bp
  20% of sequence in fragments shorter than    2813 bp
  25% of sequence in fragments shorter than    3301 bp
  30% of sequence in fragments shorter than    3793 bp
  35% of sequence in fragments shorter than    4289 bp
  40% of sequence in fragments shorter than    4798 bp
  45% of sequence in fragments shorter than    5333 bp
  50% of sequence in fragments shorter than    5899 bp
  55% of sequence in fragments shorter than    6516 bp
  60% of sequence in fragments shorter than    7186 bp
  65% of sequence in fragments shorter than    7942 bp
  70% of sequence in fragments shorter than    8786 bp
  75% of sequence in fragments shorter than    9783 bp
  80% of sequence in fragments shorter than   10988 bp
  85% of sequence in fragments shorter than   12558 bp
  90% of sequence in fragments shorter than   14848 bp
  95% of sequence in fragments shorter than   19268 bp

# AflII.KasI (CTTAAG+GGCGCC) double digest, 873 thousand fragments (643284+230381 sites), 3412 bp average fragment length
   5% of sequence in fragments shorter than    1194 bp
  10% of sequence in fragments shorter than    1823 bp
  15% of sequence in fragments shorter than    2358 bp
  20% of sequence in fragments shorter than    2857 bp
  25% of sequence in fragments shorter than    3353 bp
  30% of sequence in fragments shorter than    3821 bp
  35% of sequence in fragments shorter than    4306 bp
  40% of sequence in fragments shorter than    4815 bp
  45% of sequence in fragments shorter than    5347 bp
  50% of sequence in fragments shorter than    5913 bp
  55% of sequence in fragments shorter than    6525 bp
  60% of sequence in fragments shorter than    7189 bp
  65% of sequence in fragments shorter than    7929 bp
  70% of sequence in fragments shorter than    8761 bp
  75% of sequence in fragments shorter than    9743 bp
  80% of sequence in fragments shorter than   10934 bp
  85% of sequence in fragments shorter than   12504 bp
  90% of sequence in fragments shorter than   14821 bp
  95% of sequence in fragments shorter than   19568 bp

# ApaLI.SapI (GTGCAC+GCTCTTC) double digest, 873 thousand fragments (496255+377161 sites), 3413 bp average fragment length
   5% of sequence in fragments shorter than    1191 bp
  10% of sequence in fragments shorter than    1803 bp
  15% of sequence in fragments shorter than    2344 bp
  20% of sequence in fragments shorter than    2849 bp
  25% of sequence in fragments shorter than    3350 bp
  30% of sequence in fragments shorter than    3802 bp
  35% of sequence in fragments shorter than    4313 bp
  40% of sequence in fragments shorter than    4818 bp
  45% of sequence in fragments shorter than    5374 bp
  50% of sequence in fragments shorter than    5960 bp
  55% of sequence in fragments shorter than    6597 bp
  60% of sequence in fragments shorter than    7294 bp
  65% of sequence in fragments shorter than    8063 bp
  70% of sequence in fragments shorter than    8956 bp
  75% of sequence in fragments shorter than    9985 bp
  80% of sequence in fragments shorter than   11258 bp
  85% of sequence in fragments shorter than   12937 bp
  90% of sequence in fragments shorter than   15365 bp
  95% of sequence in fragments shorter than   20123 bp

# BsaAI.PacI (YACGTR+TTAATTAA) double digest, 872 thousand fragments (713801+159455 sites), 3414 bp average fragment length
   5% of sequence in fragments shorter than    1221 bp
  10% of sequence in fragments shorter than    1854 bp
  15% of sequence in fragments shorter than    2400 bp
  20% of sequence in fragments shorter than    2918 bp
  25% of sequence in fragments shorter than    3419 bp
  30% of sequence in fragments shorter than    3901 bp
  35% of sequence in fragments shorter than    4414 bp
  40% of sequence in fragments shorter than    4934 bp
  45% of sequence in fragments shorter than    5490 bp
  50% of sequence in fragments shorter than    6082 bp
  55% of sequence in fragments shorter than    6715 bp
  60% of sequence in fragments shorter than    7398 bp
  65% of sequence in fragments shorter than    8163 bp
  70% of sequence in fragments shorter than    9013 bp
  75% of sequence in fragments shorter than   10014 bp
  80% of sequence in fragments shorter than   11221 bp
  85% of sequence in fragments shorter than   12769 bp
  90% of sequence in fragments shorter than   14998 bp
  95% of sequence in fragments shorter than   19082 bp

# ApaLI.SmaI (GTGCAC+CCCGGG) double digest, 872 thousand fragments (496255+376940 sites), 3414 bp average fragment length
   5% of sequence in fragments shorter than    1141 bp
  10% of sequence in fragments shorter than    1798 bp
  15% of sequence in fragments shorter than    2385 bp
  20% of sequence in fragments shorter than    2954 bp
  25% of sequence in fragments shorter than    3525 bp
  30% of sequence in fragments shorter than    4112 bp
  35% of sequence in fragments shorter than    4722 bp
  40% of sequence in fragments shorter than    5365 bp
  45% of sequence in fragments shorter than    6023 bp
  50% of sequence in fragments shorter than    6753 bp
  55% of sequence in fragments shorter than    7556 bp
  60% of sequence in fragments shorter than    8440 bp
  65% of sequence in fragments shorter than    9432 bp
  70% of sequence in fragments shorter than   10572 bp
  75% of sequence in fragments shorter than   11905 bp
  80% of sequence in fragments shorter than   13612 bp
  85% of sequence in fragments shorter than   15836 bp
  90% of sequence in fragments shorter than   19153 bp
  95% of sequence in fragments shorter than   25661 bp

# MluI.XbaI (ACGCGT+TCTAGA) double digest, 871 thousand fragments (21415+850998 sites), 3417 bp average fragment length
   5% of sequence in fragments shorter than    1164 bp
  10% of sequence in fragments shorter than    1762 bp
  15% of sequence in fragments shorter than    2295 bp
  20% of sequence in fragments shorter than    2827 bp
  25% of sequence in fragments shorter than    3336 bp
  30% of sequence in fragments shorter than    3845 bp
  35% of sequence in fragments shorter than    4363 bp
  40% of sequence in fragments shorter than    4894 bp
  45% of sequence in fragments shorter than    5448 bp
  50% of sequence in fragments shorter than    6035 bp
  55% of sequence in fragments shorter than    6666 bp
  60% of sequence in fragments shorter than    7356 bp
  65% of sequence in fragments shorter than    8113 bp
  70% of sequence in fragments shorter than    8973 bp
  75% of sequence in fragments shorter than    9988 bp
  80% of sequence in fragments shorter than   11242 bp
  85% of sequence in fragments shorter than   12820 bp
  90% of sequence in fragments shorter than   15071 bp
  95% of sequence in fragments shorter than   19104 bp

# BsaAI.Hpy99I (YACGTR+CGWCG) double digest, 871 thousand fragments (713801+157917 sites), 3420 bp average fragment length
   5% of sequence in fragments shorter than    1183 bp
  10% of sequence in fragments shorter than    1852 bp
  15% of sequence in fragments shorter than    2436 bp
  20% of sequence in fragments shorter than    2997 bp
  25% of sequence in fragments shorter than    3523 bp
  30% of sequence in fragments shorter than    4082 bp
  35% of sequence in fragments shorter than    4637 bp
  40% of sequence in fragments shorter than    5225 bp
  45% of sequence in fragments shorter than    5837 bp
  50% of sequence in fragments shorter than    6492 bp
  55% of sequence in fragments shorter than    7190 bp
  60% of sequence in fragments shorter than    7971 bp
  65% of sequence in fragments shorter than    8830 bp
  70% of sequence in fragments shorter than    9800 bp
  75% of sequence in fragments shorter than   10939 bp
  80% of sequence in fragments shorter than   12322 bp
  85% of sequence in fragments shorter than   14127 bp
  90% of sequence in fragments shorter than   16653 bp
  95% of sequence in fragments shorter than   21218 bp

# BclI.SnaBI (TGATCA+TACGTA) double digest, 870 thousand fragments (738785+132299 sites), 3422 bp average fragment length
   5% of sequence in fragments shorter than    1211 bp
  10% of sequence in fragments shorter than    1809 bp
  15% of sequence in fragments shorter than    2341 bp
  20% of sequence in fragments shorter than    2814 bp
  25% of sequence in fragments shorter than    3297 bp
  30% of sequence in fragments shorter than    3785 bp
  35% of sequence in fragments shorter than    4268 bp
  40% of sequence in fragments shorter than    4773 bp
  45% of sequence in fragments shorter than    5297 bp
  50% of sequence in fragments shorter than    5851 bp
  55% of sequence in fragments shorter than    6454 bp
  60% of sequence in fragments shorter than    7105 bp
  65% of sequence in fragments shorter than    7834 bp
  70% of sequence in fragments shorter than    8670 bp
  75% of sequence in fragments shorter than    9657 bp
  80% of sequence in fragments shorter than   10870 bp
  85% of sequence in fragments shorter than   12438 bp
  90% of sequence in fragments shorter than   14758 bp
  95% of sequence in fragments shorter than   19578 bp

# HindIII.NotI (AAGCTT+GCGGCCGC) double digest, 869 thousand fragments (860365+9795 sites), 3426 bp average fragment length
   5% of sequence in fragments shorter than    1202 bp
  10% of sequence in fragments shorter than    1828 bp
  15% of sequence in fragments shorter than    2321 bp
  20% of sequence in fragments shorter than    2826 bp
  25% of sequence in fragments shorter than    3315 bp
  30% of sequence in fragments shorter than    3810 bp
  35% of sequence in fragments shorter than    4310 bp
  40% of sequence in fragments shorter than    4821 bp
  45% of sequence in fragments shorter than    5359 bp
  50% of sequence in fragments shorter than    5931 bp
  55% of sequence in fragments shorter than    6555 bp
  60% of sequence in fragments shorter than    7234 bp
  65% of sequence in fragments shorter than    7995 bp
  70% of sequence in fragments shorter than    8846 bp
  75% of sequence in fragments shorter than    9854 bp
  80% of sequence in fragments shorter than   11077 bp
  85% of sequence in fragments shorter than   12669 bp
  90% of sequence in fragments shorter than   15007 bp
  95% of sequence in fragments shorter than   19547 bp

# HindIII.RsrII (AAGCTT+CGGWCCG) double digest, 869 thousand fragments (860365+9764 sites), 3426 bp average fragment length
   5% of sequence in fragments shorter than    1201 bp
  10% of sequence in fragments shorter than    1826 bp
  15% of sequence in fragments shorter than    2320 bp
  20% of sequence in fragments shorter than    2824 bp
  25% of sequence in fragments shorter than    3314 bp
  30% of sequence in fragments shorter than    3808 bp
  35% of sequence in fragments shorter than    4308 bp
  40% of sequence in fragments shorter than    4819 bp
  45% of sequence in fragments shorter than    5357 bp
  50% of sequence in fragments shorter than    5929 bp
  55% of sequence in fragments shorter than    6554 bp
  60% of sequence in fragments shorter than    7231 bp
  65% of sequence in fragments shorter than    7994 bp
  70% of sequence in fragments shorter than    8845 bp
  75% of sequence in fragments shorter than    9855 bp
  80% of sequence in fragments shorter than   11082 bp
  85% of sequence in fragments shorter than   12674 bp
  90% of sequence in fragments shorter than   15019 bp
  95% of sequence in fragments shorter than   19572 bp

# AflII.SwaI (CTTAAG+ATTTAAAT) double digest, 869 thousand fragments (643284+226602 sites), 3427 bp average fragment length
   5% of sequence in fragments shorter than    1190 bp
  10% of sequence in fragments shorter than    1808 bp
  15% of sequence in fragments shorter than    2336 bp
  20% of sequence in fragments shorter than    2833 bp
  25% of sequence in fragments shorter than    3326 bp
  30% of sequence in fragments shorter than    3812 bp
  35% of sequence in fragments shorter than    4312 bp
  40% of sequence in fragments shorter than    4830 bp
  45% of sequence in fragments shorter than    5377 bp
  50% of sequence in fragments shorter than    5958 bp
  55% of sequence in fragments shorter than    6598 bp
  60% of sequence in fragments shorter than    7313 bp
  65% of sequence in fragments shorter than    8106 bp
  70% of sequence in fragments shorter than    9032 bp
  75% of sequence in fragments shorter than   10135 bp
  80% of sequence in fragments shorter than   11529 bp
  85% of sequence in fragments shorter than   13418 bp
  90% of sequence in fragments shorter than   16393 bp
  95% of sequence in fragments shorter than   23579 bp

# HgaI.SexAI (GACGC+ACCWGGT) double digest, 869 thousand fragments (571889+297900 sites), 3427 bp average fragment length
   5% of sequence in fragments shorter than    1149 bp
  10% of sequence in fragments shorter than    1856 bp
  15% of sequence in fragments shorter than    2497 bp
  20% of sequence in fragments shorter than    3105 bp
  25% of sequence in fragments shorter than    3717 bp
  30% of sequence in fragments shorter than    4347 bp
  35% of sequence in fragments shorter than    5007 bp
  40% of sequence in fragments shorter than    5696 bp
  45% of sequence in fragments shorter than    6422 bp
  50% of sequence in fragments shorter than    7201 bp
  55% of sequence in fragments shorter than    8049 bp
  60% of sequence in fragments shorter than    8974 bp
  65% of sequence in fragments shorter than   10033 bp
  70% of sequence in fragments shorter than   11241 bp
  75% of sequence in fragments shorter than   12669 bp
  80% of sequence in fragments shorter than   14392 bp
  85% of sequence in fragments shorter than   16639 bp
  90% of sequence in fragments shorter than   19949 bp
  95% of sequence in fragments shorter than   26190 bp

# EciI.SexAI (GGCGGA+ACCWGGT) double digest, 868 thousand fragments (571273+297900 sites), 3429 bp average fragment length
   5% of sequence in fragments shorter than    1135 bp
  10% of sequence in fragments shorter than    1803 bp
  15% of sequence in fragments shorter than    2405 bp
  20% of sequence in fragments shorter than    2991 bp
  25% of sequence in fragments shorter than    3593 bp
  30% of sequence in fragments shorter than    4214 bp
  35% of sequence in fragments shorter than    4866 bp
  40% of sequence in fragments shorter than    5562 bp
  45% of sequence in fragments shorter than    6311 bp
  50% of sequence in fragments shorter than    7118 bp
  55% of sequence in fragments shorter than    8003 bp
  60% of sequence in fragments shorter than    8987 bp
  65% of sequence in fragments shorter than   10116 bp
  70% of sequence in fragments shorter than   11429 bp
  75% of sequence in fragments shorter than   12989 bp
  80% of sequence in fragments shorter than   14940 bp
  85% of sequence in fragments shorter than   17536 bp
  90% of sequence in fragments shorter than   21466 bp
  95% of sequence in fragments shorter than   29385 bp

# AclI.BciVI (AACGTT+GTATCC) double digest, 868 thousand fragments (172706+696093 sites), 3431 bp average fragment length
   5% of sequence in fragments shorter than    1197 bp
  10% of sequence in fragments shorter than    1807 bp
  15% of sequence in fragments shorter than    2320 bp
  20% of sequence in fragments shorter than    2822 bp
  25% of sequence in fragments shorter than    3317 bp
  30% of sequence in fragments shorter than    3808 bp
  35% of sequence in fragments shorter than    4300 bp
  40% of sequence in fragments shorter than    4814 bp
  45% of sequence in fragments shorter than    5354 bp
  50% of sequence in fragments shorter than    5920 bp
  55% of sequence in fragments shorter than    6529 bp
  60% of sequence in fragments shorter than    7190 bp
  65% of sequence in fragments shorter than    7930 bp
  70% of sequence in fragments shorter than    8755 bp
  75% of sequence in fragments shorter than    9719 bp
  80% of sequence in fragments shorter than   10899 bp
  85% of sequence in fragments shorter than   12421 bp
  90% of sequence in fragments shorter than   14600 bp
  95% of sequence in fragments shorter than   18614 bp

# EcoRI.MluI (GAATTC+ACGCGT) double digest, 868 thousand fragments (847401+21415 sites), 3431 bp average fragment length
   5% of sequence in fragments shorter than    1173 bp
  10% of sequence in fragments shorter than    1851 bp
  15% of sequence in fragments shorter than    2428 bp
  20% of sequence in fragments shorter than    2959 bp
  25% of sequence in fragments shorter than    3469 bp
  30% of sequence in fragments shorter than    3987 bp
  35% of sequence in fragments shorter than    4508 bp
  40% of sequence in fragments shorter than    5049 bp
  45% of sequence in fragments shorter than    5612 bp
  50% of sequence in fragments shorter than    6206 bp
  55% of sequence in fragments shorter than    6847 bp
  60% of sequence in fragments shorter than    7548 bp
  65% of sequence in fragments shorter than    8318 bp
  70% of sequence in fragments shorter than    9185 bp
  75% of sequence in fragments shorter than   10206 bp
  80% of sequence in fragments shorter than   11436 bp
  85% of sequence in fragments shorter than   13007 bp
  90% of sequence in fragments shorter than   15248 bp
  95% of sequence in fragments shorter than   19221 bp

# FnuDII.SnaBI (CGCG+TACGTA) double digest, 865 thousand fragments (733940+132299 sites), 3441 bp average fragment length
   5% of sequence in fragments shorter than    1480 bp
  10% of sequence in fragments shorter than    2553 bp
  15% of sequence in fragments shorter than    3500 bp
  20% of sequence in fragments shorter than    4456 bp
  25% of sequence in fragments shorter than    5416 bp
  30% of sequence in fragments shorter than    6400 bp
  35% of sequence in fragments shorter than    7435 bp
  40% of sequence in fragments shorter than    8525 bp
  45% of sequence in fragments shorter than    9704 bp
  50% of sequence in fragments shorter than   10972 bp
  55% of sequence in fragments shorter than   12378 bp
  60% of sequence in fragments shorter than   13936 bp
  65% of sequence in fragments shorter than   15701 bp
  70% of sequence in fragments shorter than   17706 bp
  75% of sequence in fragments shorter than   20111 bp
  80% of sequence in fragments shorter than   23077 bp
  85% of sequence in fragments shorter than   26924 bp
  90% of sequence in fragments shorter than   32591 bp
  95% of sequence in fragments shorter than   43440 bp

# SgrAI.XbaI (CRCCGGYG+TCTAGA) double digest, 865 thousand fragments (15270+850998 sites), 3441 bp average fragment length
   5% of sequence in fragments shorter than    1173 bp
  10% of sequence in fragments shorter than    1773 bp
  15% of sequence in fragments shorter than    2311 bp
  20% of sequence in fragments shorter than    2848 bp
  25% of sequence in fragments shorter than    3360 bp
  30% of sequence in fragments shorter than    3872 bp
  35% of sequence in fragments shorter than    4394 bp
  40% of sequence in fragments shorter than    4929 bp
  45% of sequence in fragments shorter than    5486 bp
  50% of sequence in fragments shorter than    6079 bp
  55% of sequence in fragments shorter than    6715 bp
  60% of sequence in fragments shorter than    7412 bp
  65% of sequence in fragments shorter than    8180 bp
  70% of sequence in fragments shorter than    9049 bp
  75% of sequence in fragments shorter than   10082 bp
  80% of sequence in fragments shorter than   11347 bp
  85% of sequence in fragments shorter than   12928 bp
  90% of sequence in fragments shorter than   15222 bp
  95% of sequence in fragments shorter than   19352 bp

# NruI.XbaI (TCGCGA+TCTAGA) double digest, 865 thousand fragments (14872+850998 sites), 3443 bp average fragment length
   5% of sequence in fragments shorter than    1172 bp
  10% of sequence in fragments shorter than    1773 bp
  15% of sequence in fragments shorter than    2311 bp
  20% of sequence in fragments shorter than    2847 bp
  25% of sequence in fragments shorter than    3361 bp
  30% of sequence in fragments shorter than    3874 bp
  35% of sequence in fragments shorter than    4398 bp
  40% of sequence in fragments shorter than    4934 bp
  45% of sequence in fragments shorter than    5493 bp
  50% of sequence in fragments shorter than    6085 bp
  55% of sequence in fragments shorter than    6725 bp
  60% of sequence in fragments shorter than    7423 bp
  65% of sequence in fragments shorter than    8193 bp
  70% of sequence in fragments shorter than    9062 bp
  75% of sequence in fragments shorter than   10101 bp
  80% of sequence in fragments shorter than   11369 bp
  85% of sequence in fragments shorter than   12977 bp
  90% of sequence in fragments shorter than   15294 bp
  95% of sequence in fragments shorter than   19486 bp

# BsrBI.HaeII (CCGCTC+RGCGCY) double digest, 865 thousand fragments (235523+630101 sites), 3444 bp average fragment length
   5% of sequence in fragments shorter than    1033 bp
  10% of sequence in fragments shorter than    1921 bp
  15% of sequence in fragments shorter than    2769 bp
  20% of sequence in fragments shorter than    3647 bp
  25% of sequence in fragments shorter than    4540 bp
  30% of sequence in fragments shorter than    5476 bp
  35% of sequence in fragments shorter than    6459 bp
  40% of sequence in fragments shorter than    7504 bp
  45% of sequence in fragments shorter than    8654 bp
  50% of sequence in fragments shorter than    9886 bp
  55% of sequence in fragments shorter than   11251 bp
  60% of sequence in fragments shorter than   12758 bp
  65% of sequence in fragments shorter than   14496 bp
  70% of sequence in fragments shorter than   16462 bp
  75% of sequence in fragments shorter than   18852 bp
  80% of sequence in fragments shorter than   21800 bp
  85% of sequence in fragments shorter than   25571 bp
  90% of sequence in fragments shorter than   30968 bp
  95% of sequence in fragments shorter than   40169 bp

# PvuI.XbaI (CGATCG+TCTAGA) double digest, 864 thousand fragments (14186+850998 sites), 3446 bp average fragment length
   5% of sequence in fragments shorter than    1171 bp
  10% of sequence in fragments shorter than    1777 bp
  15% of sequence in fragments shorter than    2315 bp
  20% of sequence in fragments shorter than    2856 bp
  25% of sequence in fragments shorter than    3371 bp
  30% of sequence in fragments shorter than    3884 bp
  35% of sequence in fragments shorter than    4411 bp
  40% of sequence in fragments shorter than    4948 bp
  45% of sequence in fragments shorter than    5509 bp
  50% of sequence in fragments shorter than    6105 bp
  55% of sequence in fragments shorter than    6748 bp
  60% of sequence in fragments shorter than    7447 bp
  65% of sequence in fragments shorter than    8227 bp
  70% of sequence in fragments shorter than    9097 bp
  75% of sequence in fragments shorter than   10142 bp
  80% of sequence in fragments shorter than   11416 bp
  85% of sequence in fragments shorter than   13023 bp
  90% of sequence in fragments shorter than   15359 bp
  95% of sequence in fragments shorter than   19587 bp

# AscI.HindIII (GGCGCGCC+AAGCTT) double digest, 864 thousand fragments (4751+860365 sites), 3446 bp average fragment length
   5% of sequence in fragments shorter than    1208 bp
  10% of sequence in fragments shorter than    1837 bp
  15% of sequence in fragments shorter than    2334 bp
  20% of sequence in fragments shorter than    2840 bp
  25% of sequence in fragments shorter than    3333 bp
  30% of sequence in fragments shorter than    3830 bp
  35% of sequence in fragments shorter than    4333 bp
  40% of sequence in fragments shorter than    4848 bp
  45% of sequence in fragments shorter than    5390 bp
  50% of sequence in fragments shorter than    5968 bp
  55% of sequence in fragments shorter than    6596 bp
  60% of sequence in fragments shorter than    7281 bp
  65% of sequence in fragments shorter than    8048 bp
  70% of sequence in fragments shorter than    8910 bp
  75% of sequence in fragments shorter than    9930 bp
  80% of sequence in fragments shorter than   11174 bp
  85% of sequence in fragments shorter than   12790 bp
  90% of sequence in fragments shorter than   15180 bp
  95% of sequence in fragments shorter than   19906 bp

# FseI.XbaI (GGCCGGCC+TCTAGA) double digest, 864 thousand fragments (13662+850998 sites), 3448 bp average fragment length
   5% of sequence in fragments shorter than    1176 bp
  10% of sequence in fragments shorter than    1778 bp
  15% of sequence in fragments shorter than    2315 bp
  20% of sequence in fragments shorter than    2854 bp
  25% of sequence in fragments shorter than    3368 bp
  30% of sequence in fragments shorter than    3878 bp
  35% of sequence in fragments shorter than    4402 bp
  40% of sequence in fragments shorter than    4938 bp
  45% of sequence in fragments shorter than    5497 bp
  50% of sequence in fragments shorter than    6090 bp
  55% of sequence in fragments shorter than    6727 bp
  60% of sequence in fragments shorter than    7424 bp
  65% of sequence in fragments shorter than    8193 bp
  70% of sequence in fragments shorter than    9062 bp
  75% of sequence in fragments shorter than   10093 bp
  80% of sequence in fragments shorter than   11357 bp
  85% of sequence in fragments shorter than   12929 bp
  90% of sequence in fragments shorter than   15205 bp
  95% of sequence in fragments shorter than   19328 bp

# BceAI.PacI (ACGGC+TTAATTAA) double digest, 864 thousand fragments (705178+159455 sites), 3448 bp average fragment length
   5% of sequence in fragments shorter than    1172 bp
  10% of sequence in fragments shorter than    1875 bp
  15% of sequence in fragments shorter than    2508 bp
  20% of sequence in fragments shorter than    3109 bp
  25% of sequence in fragments shorter than    3717 bp
  30% of sequence in fragments shorter than    4327 bp
  35% of sequence in fragments shorter than    4960 bp
  40% of sequence in fragments shorter than    5614 bp
  45% of sequence in fragments shorter than    6299 bp
  50% of sequence in fragments shorter than    7042 bp
  55% of sequence in fragments shorter than    7832 bp
  60% of sequence in fragments shorter than    8703 bp
  65% of sequence in fragments shorter than    9669 bp
  70% of sequence in fragments shorter than   10786 bp
  75% of sequence in fragments shorter than   12117 bp
  80% of sequence in fragments shorter than   13717 bp
  85% of sequence in fragments shorter than   15788 bp
  90% of sequence in fragments shorter than   18735 bp
  95% of sequence in fragments shorter than   24408 bp

# BclI.NaeI (TGATCA+GCCGGC) double digest, 864 thousand fragments (738785+125692 sites), 3448 bp average fragment length
   5% of sequence in fragments shorter than    1223 bp
  10% of sequence in fragments shorter than    1845 bp
  15% of sequence in fragments shorter than    2390 bp
  20% of sequence in fragments shorter than    2878 bp
  25% of sequence in fragments shorter than    3368 bp
  30% of sequence in fragments shorter than    3862 bp
  35% of sequence in fragments shorter than    4350 bp
  40% of sequence in fragments shorter than    4856 bp
  45% of sequence in fragments shorter than    5379 bp
  50% of sequence in fragments shorter than    5940 bp
  55% of sequence in fragments shorter than    6540 bp
  60% of sequence in fragments shorter than    7196 bp
  65% of sequence in fragments shorter than    7917 bp
  70% of sequence in fragments shorter than    8740 bp
  75% of sequence in fragments shorter than    9695 bp
  80% of sequence in fragments shorter than   10874 bp
  85% of sequence in fragments shorter than   12385 bp
  90% of sequence in fragments shorter than   14608 bp
  95% of sequence in fragments shorter than   19055 bp

# BceAI.Hpy99I (ACGGC+CGWCG) double digest, 862 thousand fragments (705178+157917 sites), 3454 bp average fragment length
   5% of sequence in fragments shorter than    1157 bp
  10% of sequence in fragments shorter than    1947 bp
  15% of sequence in fragments shorter than    2679 bp
  20% of sequence in fragments shorter than    3393 bp
  25% of sequence in fragments shorter than    4119 bp
  30% of sequence in fragments shorter than    4859 bp
  35% of sequence in fragments shorter than    5635 bp
  40% of sequence in fragments shorter than    6442 bp
  45% of sequence in fragments shorter than    7320 bp
  50% of sequence in fragments shorter than    8261 bp
  55% of sequence in fragments shorter than    9279 bp
  60% of sequence in fragments shorter than   10410 bp
  65% of sequence in fragments shorter than   11711 bp
  70% of sequence in fragments shorter than   13161 bp
  75% of sequence in fragments shorter than   14902 bp
  80% of sequence in fragments shorter than   17039 bp
  85% of sequence in fragments shorter than   19822 bp
  90% of sequence in fragments shorter than   23827 bp
  95% of sequence in fragments shorter than   31124 bp

# EcoRI.SgrAI (GAATTC+CRCCGGYG) double digest, 862 thousand fragments (847401+15270 sites), 3456 bp average fragment length
   5% of sequence in fragments shorter than    1181 bp
  10% of sequence in fragments shorter than    1864 bp
  15% of sequence in fragments shorter than    2446 bp
  20% of sequence in fragments shorter than    2981 bp
  25% of sequence in fragments shorter than    3493 bp
  30% of sequence in fragments shorter than    4017 bp
  35% of sequence in fragments shorter than    4542 bp
  40% of sequence in fragments shorter than    5092 bp
  45% of sequence in fragments shorter than    5661 bp
  50% of sequence in fragments shorter than    6258 bp
  55% of sequence in fragments shorter than    6909 bp
  60% of sequence in fragments shorter than    7615 bp
  65% of sequence in fragments shorter than    8389 bp
  70% of sequence in fragments shorter than    9268 bp
  75% of sequence in fragments shorter than   10298 bp
  80% of sequence in fragments shorter than   11536 bp
  85% of sequence in fragments shorter than   13118 bp
  90% of sequence in fragments shorter than   15372 bp
  95% of sequence in fragments shorter than   19405 bp

# ApaLI.BamHI (GTGCAC+GGATCC) double digest, 861 thousand fragments (496255+365999 sites), 3457 bp average fragment length
   5% of sequence in fragments shorter than    1171 bp
  10% of sequence in fragments shorter than    1801 bp
  15% of sequence in fragments shorter than    2350 bp
  20% of sequence in fragments shorter than    2884 bp
  25% of sequence in fragments shorter than    3406 bp
  30% of sequence in fragments shorter than    3934 bp
  35% of sequence in fragments shorter than    4470 bp
  40% of sequence in fragments shorter than    5002 bp
  45% of sequence in fragments shorter than    5578 bp
  50% of sequence in fragments shorter than    6189 bp
  55% of sequence in fragments shorter than    6864 bp
  60% of sequence in fragments shorter than    7600 bp
  65% of sequence in fragments shorter than    8415 bp
  70% of sequence in fragments shorter than    9356 bp
  75% of sequence in fragments shorter than   10461 bp
  80% of sequence in fragments shorter than   11797 bp
  85% of sequence in fragments shorter than   13581 bp
  90% of sequence in fragments shorter than   16155 bp
  95% of sequence in fragments shorter than   21073 bp

# EcoRI.NruI (GAATTC+TCGCGA) double digest, 861 thousand fragments (847401+14872 sites), 3457 bp average fragment length
   5% of sequence in fragments shorter than    1180 bp
  10% of sequence in fragments shorter than    1864 bp
  15% of sequence in fragments shorter than    2446 bp
  20% of sequence in fragments shorter than    2981 bp
  25% of sequence in fragments shorter than    3493 bp
  30% of sequence in fragments shorter than    4016 bp
  35% of sequence in fragments shorter than    4540 bp
  40% of sequence in fragments shorter than    5091 bp
  45% of sequence in fragments shorter than    5660 bp
  50% of sequence in fragments shorter than    6259 bp
  55% of sequence in fragments shorter than    6911 bp
  60% of sequence in fragments shorter than    7617 bp
  65% of sequence in fragments shorter than    8392 bp
  70% of sequence in fragments shorter than    9273 bp
  75% of sequence in fragments shorter than   10307 bp
  80% of sequence in fragments shorter than   11547 bp
  85% of sequence in fragments shorter than   13145 bp
  90% of sequence in fragments shorter than   15427 bp
  95% of sequence in fragments shorter than   19545 bp

# MfeI.SexAI (CAATTG+ACCWGGT) double digest, 861 thousand fragments (564303+297900 sites), 3458 bp average fragment length
   5% of sequence in fragments shorter than    1206 bp
  10% of sequence in fragments shorter than    1836 bp
  15% of sequence in fragments shorter than    2352 bp
  20% of sequence in fragments shorter than    2850 bp
  25% of sequence in fragments shorter than    3334 bp
  30% of sequence in fragments shorter than    3826 bp
  35% of sequence in fragments shorter than    4312 bp
  40% of sequence in fragments shorter than    4806 bp
  45% of sequence in fragments shorter than    5330 bp
  50% of sequence in fragments shorter than    5888 bp
  55% of sequence in fragments shorter than    6488 bp
  60% of sequence in fragments shorter than    7147 bp
  65% of sequence in fragments shorter than    7871 bp
  70% of sequence in fragments shorter than    8703 bp
  75% of sequence in fragments shorter than    9667 bp
  80% of sequence in fragments shorter than   10861 bp
  85% of sequence in fragments shorter than   12396 bp
  90% of sequence in fragments shorter than   14559 bp
  95% of sequence in fragments shorter than   18615 bp

# EcoRI.PvuI (GAATTC+CGATCG) double digest, 861 thousand fragments (847401+14186 sites), 3460 bp average fragment length
   5% of sequence in fragments shorter than    1182 bp
  10% of sequence in fragments shorter than    1867 bp
  15% of sequence in fragments shorter than    2451 bp
  20% of sequence in fragments shorter than    2988 bp
  25% of sequence in fragments shorter than    3503 bp
  30% of sequence in fragments shorter than    4027 bp
  35% of sequence in fragments shorter than    4553 bp
  40% of sequence in fragments shorter than    5106 bp
  45% of sequence in fragments shorter than    5679 bp
  50% of sequence in fragments shorter than    6281 bp
  55% of sequence in fragments shorter than    6937 bp
  60% of sequence in fragments shorter than    7644 bp
  65% of sequence in fragments shorter than    8427 bp
  70% of sequence in fragments shorter than    9310 bp
  75% of sequence in fragments shorter than   10349 bp
  80% of sequence in fragments shorter than   11604 bp
  85% of sequence in fragments shorter than   13211 bp
  90% of sequence in fragments shorter than   15503 bp
  95% of sequence in fragments shorter than   19634 bp

# BglII.ClaI (AGATCT+ATCGAT) double digest, 860 thousand fragments (774732+86477 sites), 3461 bp average fragment length
   5% of sequence in fragments shorter than    1203 bp
  10% of sequence in fragments shorter than    1797 bp
  15% of sequence in fragments shorter than    2334 bp
  20% of sequence in fragments shorter than    2840 bp
  25% of sequence in fragments shorter than    3340 bp
  30% of sequence in fragments shorter than    3829 bp
  35% of sequence in fragments shorter than    4328 bp
  40% of sequence in fragments shorter than    4841 bp
  45% of sequence in fragments shorter than    5372 bp
  50% of sequence in fragments shorter than    5940 bp
  55% of sequence in fragments shorter than    6556 bp
  60% of sequence in fragments shorter than    7227 bp
  65% of sequence in fragments shorter than    7976 bp
  70% of sequence in fragments shorter than    8821 bp
  75% of sequence in fragments shorter than    9829 bp
  80% of sequence in fragments shorter than   11043 bp
  85% of sequence in fragments shorter than   12643 bp
  90% of sequence in fragments shorter than   15006 bp
  95% of sequence in fragments shorter than   19836 bp

# EcoRI.FseI (GAATTC+GGCCGGCC) double digest, 860 thousand fragments (847401+13662 sites), 3462 bp average fragment length
   5% of sequence in fragments shorter than    1185 bp
  10% of sequence in fragments shorter than    1868 bp
  15% of sequence in fragments shorter than    2452 bp
  20% of sequence in fragments shorter than    2988 bp
  25% of sequence in fragments shorter than    3501 bp
  30% of sequence in fragments shorter than    4025 bp
  35% of sequence in fragments shorter than    4550 bp
  40% of sequence in fragments shorter than    5099 bp
  45% of sequence in fragments shorter than    5669 bp
  50% of sequence in fragments shorter than    6267 bp
  55% of sequence in fragments shorter than    6918 bp
  60% of sequence in fragments shorter than    7621 bp
  65% of sequence in fragments shorter than    8394 bp
  70% of sequence in fragments shorter than    9268 bp
  75% of sequence in fragments shorter than   10297 bp
  80% of sequence in fragments shorter than   11526 bp
  85% of sequence in fragments shorter than   13107 bp
  90% of sequence in fragments shorter than   15351 bp
  95% of sequence in fragments shorter than   19322 bp

# NotI.XbaI (GCGGCCGC+TCTAGA) double digest, 860 thousand fragments (9795+850998 sites), 3463 bp average fragment length
   5% of sequence in fragments shorter than    1179 bp
  10% of sequence in fragments shorter than    1785 bp
  15% of sequence in fragments shorter than    2326 bp
  20% of sequence in fragments shorter than    2868 bp
  25% of sequence in fragments shorter than    3386 bp
  30% of sequence in fragments shorter than    3900 bp
  35% of sequence in fragments shorter than    4428 bp
  40% of sequence in fragments shorter than    4969 bp
  45% of sequence in fragments shorter than    5532 bp
  50% of sequence in fragments shorter than    6130 bp
  55% of sequence in fragments shorter than    6772 bp
  60% of sequence in fragments shorter than    7475 bp
  65% of sequence in fragments shorter than    8257 bp
  70% of sequence in fragments shorter than    9131 bp
  75% of sequence in fragments shorter than   10186 bp
  80% of sequence in fragments shorter than   11464 bp
  85% of sequence in fragments shorter than   13079 bp
  90% of sequence in fragments shorter than   15407 bp
  95% of sequence in fragments shorter than   19606 bp

# RsrII.XbaI (CGGWCCG+TCTAGA) double digest, 860 thousand fragments (9764+850998 sites), 3463 bp average fragment length
   5% of sequence in fragments shorter than    1179 bp
  10% of sequence in fragments shorter than    1784 bp
  15% of sequence in fragments shorter than    2325 bp
  20% of sequence in fragments shorter than    2866 bp
  25% of sequence in fragments shorter than    3383 bp
  30% of sequence in fragments shorter than    3897 bp
  35% of sequence in fragments shorter than    4423 bp
  40% of sequence in fragments shorter than    4964 bp
  45% of sequence in fragments shorter than    5529 bp
  50% of sequence in fragments shorter than    6127 bp
  55% of sequence in fragments shorter than    6770 bp
  60% of sequence in fragments shorter than    7474 bp
  65% of sequence in fragments shorter than    8256 bp
  70% of sequence in fragments shorter than    9129 bp
  75% of sequence in fragments shorter than   10182 bp
  80% of sequence in fragments shorter than   11459 bp
  85% of sequence in fragments shorter than   13082 bp
  90% of sequence in fragments shorter than   15415 bp
  95% of sequence in fragments shorter than   19624 bp

# ApaI.SpeI (GGGCCC+ACTAGT) double digest, 860 thousand fragments (460339+400286 sites), 3464 bp average fragment length
   5% of sequence in fragments shorter than    1180 bp
  10% of sequence in fragments shorter than    1850 bp
  15% of sequence in fragments shorter than    2435 bp
  20% of sequence in fragments shorter than    2997 bp
  25% of sequence in fragments shorter than    3558 bp
  30% of sequence in fragments shorter than    4120 bp
  35% of sequence in fragments shorter than    4699 bp
  40% of sequence in fragments shorter than    5290 bp
  45% of sequence in fragments shorter than    5914 bp
  50% of sequence in fragments shorter than    6582 bp
  55% of sequence in fragments shorter than    7290 bp
  60% of sequence in fragments shorter than    8059 bp
  65% of sequence in fragments shorter than    8915 bp
  70% of sequence in fragments shorter than    9893 bp
  75% of sequence in fragments shorter than   11053 bp
  80% of sequence in fragments shorter than   12439 bp
  85% of sequence in fragments shorter than   14264 bp
  90% of sequence in fragments shorter than   16916 bp
  95% of sequence in fragments shorter than   22024 bp

# BclI.XhoI (TGATCA+CTCGAG) double digest, 859 thousand fragments (738785+121308 sites), 3466 bp average fragment length
   5% of sequence in fragments shorter than    1228 bp
  10% of sequence in fragments shorter than    1845 bp
  15% of sequence in fragments shorter than    2357 bp
  20% of sequence in fragments shorter than    2847 bp
  25% of sequence in fragments shorter than    3333 bp
  30% of sequence in fragments shorter than    3818 bp
  35% of sequence in fragments shorter than    4298 bp
  40% of sequence in fragments shorter than    4804 bp
  45% of sequence in fragments shorter than    5326 bp
  50% of sequence in fragments shorter than    5882 bp
  55% of sequence in fragments shorter than    6482 bp
  60% of sequence in fragments shorter than    7127 bp
  65% of sequence in fragments shorter than    7845 bp
  70% of sequence in fragments shorter than    8658 bp
  75% of sequence in fragments shorter than    9612 bp
  80% of sequence in fragments shorter than   10775 bp
  85% of sequence in fragments shorter than   12264 bp
  90% of sequence in fragments shorter than   14445 bp
  95% of sequence in fragments shorter than   18907 bp

# HgaI.KpnI (GACGC+GGTACC) double digest, 859 thousand fragments (571889+287974 sites), 3467 bp average fragment length
   5% of sequence in fragments shorter than    1189 bp
  10% of sequence in fragments shorter than    1844 bp
  15% of sequence in fragments shorter than    2499 bp
  20% of sequence in fragments shorter than    3122 bp
  25% of sequence in fragments shorter than    3743 bp
  30% of sequence in fragments shorter than    4382 bp
  35% of sequence in fragments shorter than    5040 bp
  40% of sequence in fragments shorter than    5731 bp
  45% of sequence in fragments shorter than    6453 bp
  50% of sequence in fragments shorter than    7223 bp
  55% of sequence in fragments shorter than    8069 bp
  60% of sequence in fragments shorter than    8991 bp
  65% of sequence in fragments shorter than   10022 bp
  70% of sequence in fragments shorter than   11217 bp
  75% of sequence in fragments shorter than   12598 bp
  80% of sequence in fragments shorter than   14300 bp
  85% of sequence in fragments shorter than   16516 bp
  90% of sequence in fragments shorter than   19726 bp
  95% of sequence in fragments shorter than   25815 bp

# FnuDII.NaeI (CGCG+GCCGGC) double digest, 859 thousand fragments (733940+125692 sites), 3468 bp average fragment length
   5% of sequence in fragments shorter than    1502 bp
  10% of sequence in fragments shorter than    2720 bp
  15% of sequence in fragments shorter than    3883 bp
  20% of sequence in fragments shorter than    5088 bp
  25% of sequence in fragments shorter than    6348 bp
  30% of sequence in fragments shorter than    7695 bp
  35% of sequence in fragments shorter than    9140 bp
  40% of sequence in fragments shorter than   10717 bp
  45% of sequence in fragments shorter than   12443 bp
  50% of sequence in fragments shorter than   14370 bp
  55% of sequence in fragments shorter than   16519 bp
  60% of sequence in fragments shorter than   18941 bp
  65% of sequence in fragments shorter than   21675 bp
  70% of sequence in fragments shorter than   24873 bp
  75% of sequence in fragments shorter than   28727 bp
  80% of sequence in fragments shorter than   33525 bp
  85% of sequence in fragments shorter than   40222 bp
  90% of sequence in fragments shorter than   49699 bp
  95% of sequence in fragments shorter than   68427 bp

# EciI.KpnI (GGCGGA+GGTACC) double digest, 858 thousand fragments (571273+287974 sites), 3469 bp average fragment length
   5% of sequence in fragments shorter than    1186 bp
  10% of sequence in fragments shorter than    1799 bp
  15% of sequence in fragments shorter than    2404 bp
  20% of sequence in fragments shorter than    2983 bp
  25% of sequence in fragments shorter than    3567 bp
  30% of sequence in fragments shorter than    4179 bp
  35% of sequence in fragments shorter than    4817 bp
  40% of sequence in fragments shorter than    5494 bp
  45% of sequence in fragments shorter than    6200 bp
  50% of sequence in fragments shorter than    6975 bp
  55% of sequence in fragments shorter than    7827 bp
  60% of sequence in fragments shorter than    8779 bp
  65% of sequence in fragments shorter than    9845 bp
  70% of sequence in fragments shorter than   11065 bp
  75% of sequence in fragments shorter than   12529 bp
  80% of sequence in fragments shorter than   14328 bp
  85% of sequence in fragments shorter than   16757 bp
  90% of sequence in fragments shorter than   20308 bp
  95% of sequence in fragments shorter than   27520 bp

# HgaI.NheI (GACGC+GCTAGC) double digest, 858 thousand fragments (571889+287384 sites), 3469 bp average fragment length
   5% of sequence in fragments shorter than    1210 bp
  10% of sequence in fragments shorter than    1874 bp
  15% of sequence in fragments shorter than    2510 bp
  20% of sequence in fragments shorter than    3102 bp
  25% of sequence in fragments shorter than    3737 bp
  30% of sequence in fragments shorter than    4383 bp
  35% of sequence in fragments shorter than    5047 bp
  40% of sequence in fragments shorter than    5726 bp
  45% of sequence in fragments shorter than    6448 bp
  50% of sequence in fragments shorter than    7203 bp
  55% of sequence in fragments shorter than    8039 bp
  60% of sequence in fragments shorter than    8958 bp
  65% of sequence in fragments shorter than    9958 bp
  70% of sequence in fragments shorter than   11127 bp
  75% of sequence in fragments shorter than   12475 bp
  80% of sequence in fragments shorter than   14135 bp
  85% of sequence in fragments shorter than   16327 bp
  90% of sequence in fragments shorter than   19361 bp
  95% of sequence in fragments shorter than   25033 bp

# EciI.NheI (GGCGGA+GCTAGC) double digest, 858 thousand fragments (571273+287384 sites), 3472 bp average fragment length
   5% of sequence in fragments shorter than    1196 bp
  10% of sequence in fragments shorter than    1838 bp
  15% of sequence in fragments shorter than    2426 bp
  20% of sequence in fragments shorter than    2973 bp
  25% of sequence in fragments shorter than    3570 bp
  30% of sequence in fragments shorter than    4181 bp
  35% of sequence in fragments shorter than    4814 bp
  40% of sequence in fragments shorter than    5480 bp
  45% of sequence in fragments shorter than    6187 bp
  50% of sequence in fragments shorter than    6954 bp
  55% of sequence in fragments shorter than    7793 bp
  60% of sequence in fragments shorter than    8705 bp
  65% of sequence in fragments shorter than    9744 bp
  70% of sequence in fragments shorter than   10941 bp
  75% of sequence in fragments shorter than   12353 bp
  80% of sequence in fragments shorter than   14108 bp
  85% of sequence in fragments shorter than   16412 bp
  90% of sequence in fragments shorter than   19750 bp
  95% of sequence in fragments shorter than   26401 bp

# EcoRI.NotI (GAATTC+GCGGCCGC) double digest, 856 thousand fragments (847401+9795 sites), 3478 bp average fragment length
   5% of sequence in fragments shorter than    1189 bp
  10% of sequence in fragments shorter than    1876 bp
  15% of sequence in fragments shorter than    2464 bp
  20% of sequence in fragments shorter than    3004 bp
  25% of sequence in fragments shorter than    3519 bp
  30% of sequence in fragments shorter than    4047 bp
  35% of sequence in fragments shorter than    4576 bp
  40% of sequence in fragments shorter than    5128 bp
  45% of sequence in fragments shorter than    5700 bp
  50% of sequence in fragments shorter than    6306 bp
  55% of sequence in fragments shorter than    6964 bp
  60% of sequence in fragments shorter than    7672 bp
  65% of sequence in fragments shorter than    8453 bp
  70% of sequence in fragments shorter than    9339 bp
  75% of sequence in fragments shorter than   10381 bp
  80% of sequence in fragments shorter than   11639 bp
  85% of sequence in fragments shorter than   13249 bp
  90% of sequence in fragments shorter than   15533 bp
  95% of sequence in fragments shorter than   19651 bp

# EcoRI.RsrII (GAATTC+CGGWCCG) double digest, 856 thousand fragments (847401+9764 sites), 3478 bp average fragment length
   5% of sequence in fragments shorter than    1189 bp
  10% of sequence in fragments shorter than    1876 bp
  15% of sequence in fragments shorter than    2462 bp
  20% of sequence in fragments shorter than    3001 bp
  25% of sequence in fragments shorter than    3515 bp
  30% of sequence in fragments shorter than    4043 bp
  35% of sequence in fragments shorter than    4571 bp
  40% of sequence in fragments shorter than    5123 bp
  45% of sequence in fragments shorter than    5696 bp
  50% of sequence in fragments shorter than    6302 bp
  55% of sequence in fragments shorter than    6958 bp
  60% of sequence in fragments shorter than    7667 bp
  65% of sequence in fragments shorter than    8450 bp
  70% of sequence in fragments shorter than    9336 bp
  75% of sequence in fragments shorter than   10377 bp
  80% of sequence in fragments shorter than   11638 bp
  85% of sequence in fragments shorter than   13246 bp
  90% of sequence in fragments shorter than   15520 bp
  95% of sequence in fragments shorter than   19629 bp

# HaeII.SwaI (RGCGCY+ATTTAAAT) double digest, 856 thousand fragments (630101+226602 sites), 3480 bp average fragment length
   5% of sequence in fragments shorter than    1088 bp
  10% of sequence in fragments shorter than    1908 bp
  15% of sequence in fragments shorter than    2622 bp
  20% of sequence in fragments shorter than    3313 bp
  25% of sequence in fragments shorter than    3987 bp
  30% of sequence in fragments shorter than    4669 bp
  35% of sequence in fragments shorter than    5362 bp
  40% of sequence in fragments shorter than    6074 bp
  45% of sequence in fragments shorter than    6830 bp
  50% of sequence in fragments shorter than    7640 bp
  55% of sequence in fragments shorter than    8521 bp
  60% of sequence in fragments shorter than    9474 bp
  65% of sequence in fragments shorter than   10534 bp
  70% of sequence in fragments shorter than   11756 bp
  75% of sequence in fragments shorter than   13170 bp
  80% of sequence in fragments shorter than   14872 bp
  85% of sequence in fragments shorter than   17097 bp
  90% of sequence in fragments shorter than   20145 bp
  95% of sequence in fragments shorter than   25455 bp

# AscI.XbaI (GGCGCGCC+TCTAGA) double digest, 855 thousand fragments (4751+850998 sites), 3484 bp average fragment length
   5% of sequence in fragments shorter than    1187 bp
  10% of sequence in fragments shorter than    1794 bp
  15% of sequence in fragments shorter than    2337 bp
  20% of sequence in fragments shorter than    2884 bp
  25% of sequence in fragments shorter than    3403 bp
  30% of sequence in fragments shorter than    3923 bp
  35% of sequence in fragments shorter than    4453 bp
  40% of sequence in fragments shorter than    4998 bp
  45% of sequence in fragments shorter than    5565 bp
  50% of sequence in fragments shorter than    6169 bp
  55% of sequence in fragments shorter than    6817 bp
  60% of sequence in fragments shorter than    7527 bp
  65% of sequence in fragments shorter than    8315 bp
  70% of sequence in fragments shorter than    9209 bp
  75% of sequence in fragments shorter than   10270 bp
  80% of sequence in fragments shorter than   11565 bp
  85% of sequence in fragments shorter than   13222 bp
  90% of sequence in fragments shorter than   15589 bp
  95% of sequence in fragments shorter than   19928 bp

# BciVI.PacI (GTATCC+TTAATTAA) double digest, 855 thousand fragments (696093+159455 sites), 3484 bp average fragment length
   5% of sequence in fragments shorter than    1244 bp
  10% of sequence in fragments shorter than    1859 bp
  15% of sequence in fragments shorter than    2389 bp
  20% of sequence in fragments shorter than    2902 bp
  25% of sequence in fragments shorter than    3404 bp
  30% of sequence in fragments shorter than    3903 bp
  35% of sequence in fragments shorter than    4404 bp
  40% of sequence in fragments shorter than    4924 bp
  45% of sequence in fragments shorter than    5472 bp
  50% of sequence in fragments shorter than    6058 bp
  55% of sequence in fragments shorter than    6687 bp
  60% of sequence in fragments shorter than    7369 bp
  65% of sequence in fragments shorter than    8142 bp
  70% of sequence in fragments shorter than    9021 bp
  75% of sequence in fragments shorter than   10044 bp
  80% of sequence in fragments shorter than   11282 bp
  85% of sequence in fragments shorter than   12939 bp
  90% of sequence in fragments shorter than   15381 bp
  95% of sequence in fragments shorter than   20116 bp

# FnuDII.XhoI (CGCG+CTCGAG) double digest, 854 thousand fragments (733940+121308 sites), 3485 bp average fragment length
   5% of sequence in fragments shorter than    1457 bp
  10% of sequence in fragments shorter than    2575 bp
  15% of sequence in fragments shorter than    3625 bp
  20% of sequence in fragments shorter than    4689 bp
  25% of sequence in fragments shorter than    5774 bp
  30% of sequence in fragments shorter than    6898 bp
  35% of sequence in fragments shorter than    8114 bp
  40% of sequence in fragments shorter than    9423 bp
  45% of sequence in fragments shorter than   10831 bp
  50% of sequence in fragments shorter than   12398 bp
  55% of sequence in fragments shorter than   14148 bp
  60% of sequence in fragments shorter than   16110 bp
  65% of sequence in fragments shorter than   18301 bp
  70% of sequence in fragments shorter than   20905 bp
  75% of sequence in fragments shorter than   23995 bp
  80% of sequence in fragments shorter than   27895 bp
  85% of sequence in fragments shorter than   33070 bp
  90% of sequence in fragments shorter than   40742 bp
  95% of sequence in fragments shorter than   55437 bp

# BciVI.Hpy99I (GTATCC+CGWCG) double digest, 853 thousand fragments (696093+157917 sites), 3491 bp average fragment length
   5% of sequence in fragments shorter than    1249 bp
  10% of sequence in fragments shorter than    1885 bp
  15% of sequence in fragments shorter than    2430 bp
  20% of sequence in fragments shorter than    2953 bp
  25% of sequence in fragments shorter than    3469 bp
  30% of sequence in fragments shorter than    3980 bp
  35% of sequence in fragments shorter than    4490 bp
  40% of sequence in fragments shorter than    5029 bp
  45% of sequence in fragments shorter than    5591 bp
  50% of sequence in fragments shorter than    6180 bp
  55% of sequence in fragments shorter than    6812 bp
  60% of sequence in fragments shorter than    7500 bp
  65% of sequence in fragments shorter than    8274 bp
  70% of sequence in fragments shorter than    9129 bp
  75% of sequence in fragments shorter than   10132 bp
  80% of sequence in fragments shorter than   11324 bp
  85% of sequence in fragments shorter than   12880 bp
  90% of sequence in fragments shorter than   15098 bp
  95% of sequence in fragments shorter than   19155 bp

# KpnI.MfeI (GGTACC+CAATTG) double digest, 851 thousand fragments (287974+564303 sites), 3498 bp average fragment length
   5% of sequence in fragments shorter than    1198 bp
  10% of sequence in fragments shorter than    1817 bp
  15% of sequence in fragments shorter than    2351 bp
  20% of sequence in fragments shorter than    2869 bp
  25% of sequence in fragments shorter than    3380 bp
  30% of sequence in fragments shorter than    3880 bp
  35% of sequence in fragments shorter than    4397 bp
  40% of sequence in fragments shorter than    4932 bp
  45% of sequence in fragments shorter than    5497 bp
  50% of sequence in fragments shorter than    6098 bp
  55% of sequence in fragments shorter than    6742 bp
  60% of sequence in fragments shorter than    7439 bp
  65% of sequence in fragments shorter than    8238 bp
  70% of sequence in fragments shorter than    9131 bp
  75% of sequence in fragments shorter than   10211 bp
  80% of sequence in fragments shorter than   11485 bp
  85% of sequence in fragments shorter than   13141 bp
  90% of sequence in fragments shorter than   15548 bp
  95% of sequence in fragments shorter than   20321 bp

# AscI.EcoRI (GGCGCGCC+GAATTC) double digest, 851 thousand fragments (4751+847401 sites), 3498 bp average fragment length
   5% of sequence in fragments shorter than    1195 bp
  10% of sequence in fragments shorter than    1887 bp
  15% of sequence in fragments shorter than    2477 bp
  20% of sequence in fragments shorter than    3019 bp
  25% of sequence in fragments shorter than    3537 bp
  30% of sequence in fragments shorter than    4069 bp
  35% of sequence in fragments shorter than    4603 bp
  40% of sequence in fragments shorter than    5159 bp
  45% of sequence in fragments shorter than    5737 bp
  50% of sequence in fragments shorter than    6347 bp
  55% of sequence in fragments shorter than    7009 bp
  60% of sequence in fragments shorter than    7724 bp
  65% of sequence in fragments shorter than    8516 bp
  70% of sequence in fragments shorter than    9409 bp
  75% of sequence in fragments shorter than   10468 bp
  80% of sequence in fragments shorter than   11747 bp
  85% of sequence in fragments shorter than   13391 bp
  90% of sequence in fragments shorter than   15715 bp
  95% of sequence in fragments shorter than   19972 bp

# MfeI.NheI (CAATTG+GCTAGC) double digest, 851 thousand fragments (564303+287384 sites), 3500 bp average fragment length
   5% of sequence in fragments shorter than    1187 bp
  10% of sequence in fragments shorter than    1830 bp
  15% of sequence in fragments shorter than    2373 bp
  20% of sequence in fragments shorter than    2868 bp
  25% of sequence in fragments shorter than    3386 bp
  30% of sequence in fragments shorter than    3911 bp
  35% of sequence in fragments shorter than    4440 bp
  40% of sequence in fragments shorter than    4991 bp
  45% of sequence in fragments shorter than    5568 bp
  50% of sequence in fragments shorter than    6188 bp
  55% of sequence in fragments shorter than    6855 bp
  60% of sequence in fragments shorter than    7591 bp
  65% of sequence in fragments shorter than    8420 bp
  70% of sequence in fragments shorter than    9364 bp
  75% of sequence in fragments shorter than   10478 bp
  80% of sequence in fragments shorter than   11827 bp
  85% of sequence in fragments shorter than   13588 bp
  90% of sequence in fragments shorter than   16193 bp
  95% of sequence in fragments shorter than   21366 bp

# ApaI.HpaI (GGGCCC+GTTAAC) double digest, 849 thousand fragments (460339+389418 sites), 3508 bp average fragment length
   5% of sequence in fragments shorter than    1181 bp
  10% of sequence in fragments shorter than    1860 bp
  15% of sequence in fragments shorter than    2471 bp
  20% of sequence in fragments shorter than    3053 bp
  25% of sequence in fragments shorter than    3627 bp
  30% of sequence in fragments shorter than    4207 bp
  35% of sequence in fragments shorter than    4805 bp
  40% of sequence in fragments shorter than    5428 bp
  45% of sequence in fragments shorter than    6081 bp
  50% of sequence in fragments shorter than    6774 bp
  55% of sequence in fragments shorter than    7522 bp
  60% of sequence in fragments shorter than    8341 bp
  65% of sequence in fragments shorter than    9253 bp
  70% of sequence in fragments shorter than   10298 bp
  75% of sequence in fragments shorter than   11526 bp
  80% of sequence in fragments shorter than   13033 bp
  85% of sequence in fragments shorter than   14991 bp
  90% of sequence in fragments shorter than   17900 bp
  95% of sequence in fragments shorter than   23722 bp

# SexAI.SphI (ACCWGGT+GCATGC) double digest, 848 thousand fragments (297900+550952 sites), 3512 bp average fragment length
   5% of sequence in fragments shorter than    1220 bp
  10% of sequence in fragments shorter than    1846 bp
  15% of sequence in fragments shorter than    2389 bp
  20% of sequence in fragments shorter than    2908 bp
  25% of sequence in fragments shorter than    3420 bp
  30% of sequence in fragments shorter than    3936 bp
  35% of sequence in fragments shorter than    4462 bp
  40% of sequence in fragments shorter than    5012 bp
  45% of sequence in fragments shorter than    5589 bp
  50% of sequence in fragments shorter than    6201 bp
  55% of sequence in fragments shorter than    6855 bp
  60% of sequence in fragments shorter than    7565 bp
  65% of sequence in fragments shorter than    8368 bp
  70% of sequence in fragments shorter than    9286 bp
  75% of sequence in fragments shorter than   10358 bp
  80% of sequence in fragments shorter than   11671 bp
  85% of sequence in fragments shorter than   13390 bp
  90% of sequence in fragments shorter than   15873 bp
  95% of sequence in fragments shorter than   20953 bp

# EcoRV.SpeI (GATATC+ACTAGT) double digest, 846 thousand fragments (446473+400286 sites), 3521 bp average fragment length
   5% of sequence in fragments shorter than    1212 bp
  10% of sequence in fragments shorter than    1840 bp
  15% of sequence in fragments shorter than    2340 bp
  20% of sequence in fragments shorter than    2863 bp
  25% of sequence in fragments shorter than    3379 bp
  30% of sequence in fragments shorter than    3897 bp
  35% of sequence in fragments shorter than    4422 bp
  40% of sequence in fragments shorter than    4969 bp
  45% of sequence in fragments shorter than    5554 bp
  50% of sequence in fragments shorter than    6176 bp
  55% of sequence in fragments shorter than    6856 bp
  60% of sequence in fragments shorter than    7603 bp
  65% of sequence in fragments shorter than    8457 bp
  70% of sequence in fragments shorter than    9432 bp
  75% of sequence in fragments shorter than   10619 bp
  80% of sequence in fragments shorter than   12102 bp
  85% of sequence in fragments shorter than   14126 bp
  90% of sequence in fragments shorter than   17271 bp
  95% of sequence in fragments shorter than   23878 bp

# ClaI.NcoI (ATCGAT+CCATGG) double digest, 845 thousand fragments (86477+759594 sites), 3523 bp average fragment length
   5% of sequence in fragments shorter than    1229 bp
  10% of sequence in fragments shorter than    1866 bp
  15% of sequence in fragments shorter than    2417 bp
  20% of sequence in fragments shorter than    2948 bp
  25% of sequence in fragments shorter than    3473 bp
  30% of sequence in fragments shorter than    3996 bp
  35% of sequence in fragments shorter than    4530 bp
  40% of sequence in fragments shorter than    5082 bp
  45% of sequence in fragments shorter than    5655 bp
  50% of sequence in fragments shorter than    6269 bp
  55% of sequence in fragments shorter than    6942 bp
  60% of sequence in fragments shorter than    7676 bp
  65% of sequence in fragments shorter than    8500 bp
  70% of sequence in fragments shorter than    9431 bp
  75% of sequence in fragments shorter than   10541 bp
  80% of sequence in fragments shorter than   11877 bp
  85% of sequence in fragments shorter than   13649 bp
  90% of sequence in fragments shorter than   16190 bp
  95% of sequence in fragments shorter than   21383 bp

# AatII.BglII (GACGTC+AGATCT) double digest, 845 thousand fragments (71089+774732 sites), 3524 bp average fragment length
   5% of sequence in fragments shorter than    1235 bp
  10% of sequence in fragments shorter than    1837 bp
  15% of sequence in fragments shorter than    2382 bp
  20% of sequence in fragments shorter than    2895 bp
  25% of sequence in fragments shorter than    3402 bp
  30% of sequence in fragments shorter than    3899 bp
  35% of sequence in fragments shorter than    4404 bp
  40% of sequence in fragments shorter than    4919 bp
  45% of sequence in fragments shorter than    5457 bp
  50% of sequence in fragments shorter than    6022 bp
  55% of sequence in fragments shorter than    6641 bp
  60% of sequence in fragments shorter than    7316 bp
  65% of sequence in fragments shorter than    8060 bp
  70% of sequence in fragments shorter than    8904 bp
  75% of sequence in fragments shorter than    9903 bp
  80% of sequence in fragments shorter than   11101 bp
  85% of sequence in fragments shorter than   12679 bp
  90% of sequence in fragments shorter than   14991 bp
  95% of sequence in fragments shorter than   19547 bp

# ScaI.SexAI (AGTACT+ACCWGGT) double digest, 841 thousand fragments (543793+297900 sites), 3542 bp average fragment length
   5% of sequence in fragments shorter than    1240 bp
  10% of sequence in fragments shorter than    1868 bp
  15% of sequence in fragments shorter than    2413 bp
  20% of sequence in fragments shorter than    2921 bp
  25% of sequence in fragments shorter than    3423 bp
  30% of sequence in fragments shorter than    3925 bp
  35% of sequence in fragments shorter than    4443 bp
  40% of sequence in fragments shorter than    4972 bp
  45% of sequence in fragments shorter than    5527 bp
  50% of sequence in fragments shorter than    6118 bp
  55% of sequence in fragments shorter than    6760 bp
  60% of sequence in fragments shorter than    7450 bp
  65% of sequence in fragments shorter than    8209 bp
  70% of sequence in fragments shorter than    9073 bp
  75% of sequence in fragments shorter than   10092 bp
  80% of sequence in fragments shorter than   11334 bp
  85% of sequence in fragments shorter than   12953 bp
  90% of sequence in fragments shorter than   15318 bp
  95% of sequence in fragments shorter than   19995 bp

# BglII.SacII (AGATCT+CCGCGG) double digest, 841 thousand fragments (774732+66901 sites), 3542 bp average fragment length
   5% of sequence in fragments shorter than    1247 bp
  10% of sequence in fragments shorter than    1873 bp
  15% of sequence in fragments shorter than    2436 bp
  20% of sequence in fragments shorter than    2969 bp
  25% of sequence in fragments shorter than    3491 bp
  30% of sequence in fragments shorter than    4006 bp
  35% of sequence in fragments shorter than    4521 bp
  40% of sequence in fragments shorter than    5049 bp
  45% of sequence in fragments shorter than    5602 bp
  50% of sequence in fragments shorter than    6195 bp
  55% of sequence in fragments shorter than    6828 bp
  60% of sequence in fragments shorter than    7530 bp
  65% of sequence in fragments shorter than    8288 bp
  70% of sequence in fragments shorter than    9163 bp
  75% of sequence in fragments shorter than   10194 bp
  80% of sequence in fragments shorter than   11416 bp
  85% of sequence in fragments shorter than   13042 bp
  90% of sequence in fragments shorter than   15403 bp
  95% of sequence in fragments shorter than   20149 bp

# BsaAI.NaeI (YACGTR+GCCGGC) double digest, 839 thousand fragments (713801+125692 sites), 3551 bp average fragment length
   5% of sequence in fragments shorter than    1236 bp
  10% of sequence in fragments shorter than    1923 bp
  15% of sequence in fragments shorter than    2525 bp
  20% of sequence in fragments shorter than    3096 bp
  25% of sequence in fragments shorter than    3632 bp
  30% of sequence in fragments shorter than    4206 bp
  35% of sequence in fragments shorter than    4771 bp
  40% of sequence in fragments shorter than    5379 bp
  45% of sequence in fragments shorter than    6016 bp
  50% of sequence in fragments shorter than    6683 bp
  55% of sequence in fragments shorter than    7400 bp
  60% of sequence in fragments shorter than    8201 bp
  65% of sequence in fragments shorter than    9078 bp
  70% of sequence in fragments shorter than   10089 bp
  75% of sequence in fragments shorter than   11252 bp
  80% of sequence in fragments shorter than   12678 bp
  85% of sequence in fragments shorter than   14499 bp
  90% of sequence in fragments shorter than   17167 bp
  95% of sequence in fragments shorter than   21965 bp

# KpnI.SphI (GGTACC+GCATGC) double digest, 838 thousand fragments (287974+550952 sites), 3553 bp average fragment length
   5% of sequence in fragments shorter than    1229 bp
  10% of sequence in fragments shorter than    1836 bp
  15% of sequence in fragments shorter than    2382 bp
  20% of sequence in fragments shorter than    2896 bp
  25% of sequence in fragments shorter than    3402 bp
  30% of sequence in fragments shorter than    3905 bp
  35% of sequence in fragments shorter than    4422 bp
  40% of sequence in fragments shorter than    4963 bp
  45% of sequence in fragments shorter than    5528 bp
  50% of sequence in fragments shorter than    6120 bp
  55% of sequence in fragments shorter than    6758 bp
  60% of sequence in fragments shorter than    7448 bp
  65% of sequence in fragments shorter than    8226 bp
  70% of sequence in fragments shorter than    9118 bp
  75% of sequence in fragments shorter than   10155 bp
  80% of sequence in fragments shorter than   11427 bp
  85% of sequence in fragments shorter than   13081 bp
  90% of sequence in fragments shorter than   15525 bp
  95% of sequence in fragments shorter than   20283 bp

# BsrBI.SacI (CCGCTC+GAGCTC) double digest, 838 thousand fragments (235523+603323 sites), 3554 bp average fragment length
   5% of sequence in fragments shorter than    1185 bp
  10% of sequence in fragments shorter than    1877 bp
  15% of sequence in fragments shorter than    2486 bp
  20% of sequence in fragments shorter than    3100 bp
  25% of sequence in fragments shorter than    3704 bp
  30% of sequence in fragments shorter than    4313 bp
  35% of sequence in fragments shorter than    4942 bp
  40% of sequence in fragments shorter than    5602 bp
  45% of sequence in fragments shorter than    6305 bp
  50% of sequence in fragments shorter than    7056 bp
  55% of sequence in fragments shorter than    7871 bp
  60% of sequence in fragments shorter than    8773 bp
  65% of sequence in fragments shorter than    9798 bp
  70% of sequence in fragments shorter than   10949 bp
  75% of sequence in fragments shorter than   12318 bp
  80% of sequence in fragments shorter than   14003 bp
  85% of sequence in fragments shorter than   16191 bp
  90% of sequence in fragments shorter than   19339 bp
  95% of sequence in fragments shorter than   25518 bp

# NheI.SphI (GCTAGC+GCATGC) double digest, 837 thousand fragments (287384+550952 sites), 3556 bp average fragment length
   5% of sequence in fragments shorter than    1278 bp
  10% of sequence in fragments shorter than    1855 bp
  15% of sequence in fragments shorter than    2410 bp
  20% of sequence in fragments shorter than    2900 bp
  25% of sequence in fragments shorter than    3418 bp
  30% of sequence in fragments shorter than    3934 bp
  35% of sequence in fragments shorter than    4461 bp
  40% of sequence in fragments shorter than    5001 bp
  45% of sequence in fragments shorter than    5566 bp
  50% of sequence in fragments shorter than    6167 bp
  55% of sequence in fragments shorter than    6808 bp
  60% of sequence in fragments shorter than    7496 bp
  65% of sequence in fragments shorter than    8262 bp
  70% of sequence in fragments shorter than    9148 bp
  75% of sequence in fragments shorter than   10172 bp
  80% of sequence in fragments shorter than   11434 bp
  85% of sequence in fragments shorter than   13046 bp
  90% of sequence in fragments shorter than   15423 bp
  95% of sequence in fragments shorter than   19973 bp

# ApaI.SapI (GGGCCC+GCTCTTC) double digest, 837 thousand fragments (460339+377161 sites), 3559 bp average fragment length
   5% of sequence in fragments shorter than    1141 bp
  10% of sequence in fragments shorter than    1868 bp
  15% of sequence in fragments shorter than    2546 bp
  20% of sequence in fragments shorter than    3201 bp
  25% of sequence in fragments shorter than    3866 bp
  30% of sequence in fragments shorter than    4536 bp
  35% of sequence in fragments shorter than    5224 bp
  40% of sequence in fragments shorter than    5953 bp
  45% of sequence in fragments shorter than    6736 bp
  50% of sequence in fragments shorter than    7563 bp
  55% of sequence in fragments shorter than    8471 bp
  60% of sequence in fragments shorter than    9468 bp
  65% of sequence in fragments shorter than   10599 bp
  70% of sequence in fragments shorter than   11883 bp
  75% of sequence in fragments shorter than   13383 bp
  80% of sequence in fragments shorter than   15295 bp
  85% of sequence in fragments shorter than   17788 bp
  90% of sequence in fragments shorter than   21596 bp
  95% of sequence in fragments shorter than   29431 bp

# BceAI.SnaBI (ACGGC+TACGTA) double digest, 837 thousand fragments (705178+132299 sites), 3560 bp average fragment length
   5% of sequence in fragments shorter than    1194 bp
  10% of sequence in fragments shorter than    1910 bp
  15% of sequence in fragments shorter than    2571 bp
  20% of sequence in fragments shorter than    3197 bp
  25% of sequence in fragments shorter than    3828 bp
  30% of sequence in fragments shorter than    4475 bp
  35% of sequence in fragments shorter than    5144 bp
  40% of sequence in fragments shorter than    5835 bp
  45% of sequence in fragments shorter than    6560 bp
  50% of sequence in fragments shorter than    7344 bp
  55% of sequence in fragments shorter than    8204 bp
  60% of sequence in fragments shorter than    9131 bp
  65% of sequence in fragments shorter than   10185 bp
  70% of sequence in fragments shorter than   11374 bp
  75% of sequence in fragments shorter than   12774 bp
  80% of sequence in fragments shorter than   14514 bp
  85% of sequence in fragments shorter than   16725 bp
  90% of sequence in fragments shorter than   19891 bp
  95% of sequence in fragments shorter than   25722 bp

# ApaI.SmaI (GGGCCC+CCCGGG) double digest, 836 thousand fragments (460339+376940 sites), 3560 bp average fragment length
   5% of sequence in fragments shorter than    1116 bp
  10% of sequence in fragments shorter than    1904 bp
  15% of sequence in fragments shorter than    2665 bp
  20% of sequence in fragments shorter than    3446 bp
  25% of sequence in fragments shorter than    4258 bp
  30% of sequence in fragments shorter than    5108 bp
  35% of sequence in fragments shorter than    6024 bp
  40% of sequence in fragments shorter than    7010 bp
  45% of sequence in fragments shorter than    8072 bp
  50% of sequence in fragments shorter than    9238 bp
  55% of sequence in fragments shorter than   10526 bp
  60% of sequence in fragments shorter than   11974 bp
  65% of sequence in fragments shorter than   13647 bp
  70% of sequence in fragments shorter than   15631 bp
  75% of sequence in fragments shorter than   17965 bp
  80% of sequence in fragments shorter than   21002 bp
  85% of sequence in fragments shorter than   24965 bp
  90% of sequence in fragments shorter than   31117 bp
  95% of sequence in fragments shorter than   44034 bp

# EcoRV.HpaI (GATATC+GTTAAC) double digest, 835 thousand fragments (446473+389418 sites), 3566 bp average fragment length
   5% of sequence in fragments shorter than    1227 bp
  10% of sequence in fragments shorter than    1880 bp
  15% of sequence in fragments shorter than    2440 bp
  20% of sequence in fragments shorter than    2973 bp
  25% of sequence in fragments shorter than    3488 bp
  30% of sequence in fragments shorter than    4010 bp
  35% of sequence in fragments shorter than    4536 bp
  40% of sequence in fragments shorter than    5087 bp
  45% of sequence in fragments shorter than    5662 bp
  50% of sequence in fragments shorter than    6283 bp
  55% of sequence in fragments shorter than    6956 bp
  60% of sequence in fragments shorter than    7700 bp
  65% of sequence in fragments shorter than    8528 bp
  70% of sequence in fragments shorter than    9481 bp
  75% of sequence in fragments shorter than   10637 bp
  80% of sequence in fragments shorter than   12071 bp
  85% of sequence in fragments shorter than   13963 bp
  90% of sequence in fragments shorter than   16912 bp
  95% of sequence in fragments shorter than   22942 bp

# BsaAI.XhoI (YACGTR+CTCGAG) double digest, 834 thousand fragments (713801+121308 sites), 3570 bp average fragment length
   5% of sequence in fragments shorter than    1253 bp
  10% of sequence in fragments shorter than    1920 bp
  15% of sequence in fragments shorter than    2499 bp
  20% of sequence in fragments shorter than    3045 bp
  25% of sequence in fragments shorter than    3558 bp
  30% of sequence in fragments shorter than    4092 bp
  35% of sequence in fragments shorter than    4632 bp
  40% of sequence in fragments shorter than    5205 bp
  45% of sequence in fragments shorter than    5804 bp
  50% of sequence in fragments shorter than    6444 bp
  55% of sequence in fragments shorter than    7124 bp
  60% of sequence in fragments shorter than    7877 bp
  65% of sequence in fragments shorter than    8705 bp
  70% of sequence in fragments shorter than    9654 bp
  75% of sequence in fragments shorter than   10755 bp
  80% of sequence in fragments shorter than   12081 bp
  85% of sequence in fragments shorter than   13795 bp
  90% of sequence in fragments shorter than   16271 bp
  95% of sequence in fragments shorter than   20636 bp

# BglII.PspXI (AGATCT+VCTCGAGB) double digest, 833 thousand fragments (774732+59193 sites), 3575 bp average fragment length
   5% of sequence in fragments shorter than    1251 bp
  10% of sequence in fragments shorter than    1862 bp
  15% of sequence in fragments shorter than    2413 bp
  20% of sequence in fragments shorter than    2933 bp
  25% of sequence in fragments shorter than    3445 bp
  30% of sequence in fragments shorter than    3949 bp
  35% of sequence in fragments shorter than    4456 bp
  40% of sequence in fragments shorter than    4971 bp
  45% of sequence in fragments shorter than    5513 bp
  50% of sequence in fragments shorter than    6093 bp
  55% of sequence in fragments shorter than    6721 bp
  60% of sequence in fragments shorter than    7405 bp
  65% of sequence in fragments shorter than    8153 bp
  70% of sequence in fragments shorter than    9005 bp
  75% of sequence in fragments shorter than   10007 bp
  80% of sequence in fragments shorter than   11226 bp
  85% of sequence in fragments shorter than   12811 bp
  90% of sequence in fragments shorter than   15140 bp
  95% of sequence in fragments shorter than   19821 bp

# KasI.SacI (GGCGCC+GAGCTC) double digest, 833 thousand fragments (230381+603323 sites), 3576 bp average fragment length
   5% of sequence in fragments shorter than    1205 bp
  10% of sequence in fragments shorter than    1857 bp
  15% of sequence in fragments shorter than    2456 bp
  20% of sequence in fragments shorter than    3041 bp
  25% of sequence in fragments shorter than    3623 bp
  30% of sequence in fragments shorter than    4212 bp
  35% of sequence in fragments shorter than    4815 bp
  40% of sequence in fragments shorter than    5447 bp
  45% of sequence in fragments shorter than    6120 bp
  50% of sequence in fragments shorter than    6840 bp
  55% of sequence in fragments shorter than    7628 bp
  60% of sequence in fragments shorter than    8496 bp
  65% of sequence in fragments shorter than    9473 bp
  70% of sequence in fragments shorter than   10601 bp
  75% of sequence in fragments shorter than   11950 bp
  80% of sequence in fragments shorter than   13598 bp
  85% of sequence in fragments shorter than   15736 bp
  90% of sequence in fragments shorter than   19001 bp
  95% of sequence in fragments shorter than   25437 bp

# ApaLI.BstEII (GTGCAC+GGTNACC) double digest, 831 thousand fragments (496255+335865 sites), 3582 bp average fragment length
   5% of sequence in fragments shorter than    1251 bp
  10% of sequence in fragments shorter than    1897 bp
  15% of sequence in fragments shorter than    2476 bp
  20% of sequence in fragments shorter than    3019 bp
  25% of sequence in fragments shorter than    3541 bp
  30% of sequence in fragments shorter than    4076 bp
  35% of sequence in fragments shorter than    4548 bp
  40% of sequence in fragments shorter than    5105 bp
  45% of sequence in fragments shorter than    5698 bp
  50% of sequence in fragments shorter than    6332 bp
  55% of sequence in fragments shorter than    7010 bp
  60% of sequence in fragments shorter than    7767 bp
  65% of sequence in fragments shorter than    8597 bp
  70% of sequence in fragments shorter than    9546 bp
  75% of sequence in fragments shorter than   10659 bp
  80% of sequence in fragments shorter than   12011 bp
  85% of sequence in fragments shorter than   13828 bp
  90% of sequence in fragments shorter than   16429 bp
  95% of sequence in fragments shorter than   21748 bp

# KpnI.ScaI (GGTACC+AGTACT) double digest, 831 thousand fragments (287974+543793 sites), 3584 bp average fragment length
   5% of sequence in fragments shorter than    1237 bp
  10% of sequence in fragments shorter than    1848 bp
  15% of sequence in fragments shorter than    2399 bp
  20% of sequence in fragments shorter than    2909 bp
  25% of sequence in fragments shorter than    3415 bp
  30% of sequence in fragments shorter than    3916 bp
  35% of sequence in fragments shorter than    4434 bp
  40% of sequence in fragments shorter than    4964 bp
  45% of sequence in fragments shorter than    5528 bp
  50% of sequence in fragments shorter than    6118 bp
  55% of sequence in fragments shorter than    6760 bp
  60% of sequence in fragments shorter than    7466 bp
  65% of sequence in fragments shorter than    8245 bp
  70% of sequence in fragments shorter than    9138 bp
  75% of sequence in fragments shorter than   10177 bp
  80% of sequence in fragments shorter than   11463 bp
  85% of sequence in fragments shorter than   13168 bp
  90% of sequence in fragments shorter than   15646 bp
  95% of sequence in fragments shorter than   20849 bp

# NheI.ScaI (GCTAGC+AGTACT) double digest, 830 thousand fragments (287384+543793 sites), 3586 bp average fragment length
   5% of sequence in fragments shorter than    1283 bp
  10% of sequence in fragments shorter than    1858 bp
  15% of sequence in fragments shorter than    2408 bp
  20% of sequence in fragments shorter than    2893 bp
  25% of sequence in fragments shorter than    3404 bp
  30% of sequence in fragments shorter than    3916 bp
  35% of sequence in fragments shorter than    4445 bp
  40% of sequence in fragments shorter than    4986 bp
  45% of sequence in fragments shorter than    5556 bp
  50% of sequence in fragments shorter than    6167 bp
  55% of sequence in fragments shorter than    6823 bp
  60% of sequence in fragments shorter than    7540 bp
  65% of sequence in fragments shorter than    8337 bp
  70% of sequence in fragments shorter than    9246 bp
  75% of sequence in fragments shorter than   10327 bp
  80% of sequence in fragments shorter than   11667 bp
  85% of sequence in fragments shorter than   13440 bp
  90% of sequence in fragments shorter than   16047 bp
  95% of sequence in fragments shorter than   21636 bp

# BceAI.NaeI (ACGGC+GCCGGC) double digest, 830 thousand fragments (705178+125692 sites), 3588 bp average fragment length
   5% of sequence in fragments shorter than    1180 bp
  10% of sequence in fragments shorter than    1990 bp
  15% of sequence in fragments shorter than    2746 bp
  20% of sequence in fragments shorter than    3486 bp
  25% of sequence in fragments shorter than    4240 bp
  30% of sequence in fragments shorter than    5020 bp
  35% of sequence in fragments shorter than    5829 bp
  40% of sequence in fragments shorter than    6678 bp
  45% of sequence in fragments shorter than    7592 bp
  50% of sequence in fragments shorter than    8581 bp
  55% of sequence in fragments shorter than    9651 bp
  60% of sequence in fragments shorter than   10859 bp
  65% of sequence in fragments shorter than   12202 bp
  70% of sequence in fragments shorter than   13752 bp
  75% of sequence in fragments shorter than   15578 bp
  80% of sequence in fragments shorter than   17820 bp
  85% of sequence in fragments shorter than   20733 bp
  90% of sequence in fragments shorter than   25054 bp
  95% of sequence in fragments shorter than   33173 bp

# AatII.NcoI (GACGTC+CCATGG) double digest, 830 thousand fragments (71089+759594 sites), 3589 bp average fragment length
   5% of sequence in fragments shorter than    1241 bp
  10% of sequence in fragments shorter than    1899 bp
  15% of sequence in fragments shorter than    2485 bp
  20% of sequence in fragments shorter than    3042 bp
  25% of sequence in fragments shorter than    3589 bp
  30% of sequence in fragments shorter than    4138 bp
  35% of sequence in fragments shorter than    4676 bp
  40% of sequence in fragments shorter than    5252 bp
  45% of sequence in fragments shorter than    5847 bp
  50% of sequence in fragments shorter than    6478 bp
  55% of sequence in fragments shorter than    7180 bp
  60% of sequence in fragments shorter than    7958 bp
  65% of sequence in fragments shorter than    8826 bp
  70% of sequence in fragments shorter than    9800 bp
  75% of sequence in fragments shorter than   10951 bp
  80% of sequence in fragments shorter than   12369 bp
  85% of sequence in fragments shorter than   14186 bp
  90% of sequence in fragments shorter than   16913 bp
  95% of sequence in fragments shorter than   22362 bp

# AvrII.BsrBI (CCTAGG+CCGCTC) double digest, 830 thousand fragments (594956+235523 sites), 3590 bp average fragment length
   5% of sequence in fragments shorter than    1220 bp
  10% of sequence in fragments shorter than    1898 bp
  15% of sequence in fragments shorter than    2514 bp
  20% of sequence in fragments shorter than    3099 bp
  25% of sequence in fragments shorter than    3665 bp
  30% of sequence in fragments shorter than    4237 bp
  35% of sequence in fragments shorter than    4816 bp
  40% of sequence in fragments shorter than    5422 bp
  45% of sequence in fragments shorter than    6063 bp
  50% of sequence in fragments shorter than    6744 bp
  55% of sequence in fragments shorter than    7486 bp
  60% of sequence in fragments shorter than    8290 bp
  65% of sequence in fragments shorter than    9200 bp
  70% of sequence in fragments shorter than   10241 bp
  75% of sequence in fragments shorter than   11471 bp
  80% of sequence in fragments shorter than   12985 bp
  85% of sequence in fragments shorter than   14922 bp
  90% of sequence in fragments shorter than   17769 bp
  95% of sequence in fragments shorter than   23439 bp

# SacI.SwaI (GAGCTC+ATTTAAAT) double digest, 829 thousand fragments (603323+226602 sites), 3592 bp average fragment length
   5% of sequence in fragments shorter than    1260 bp
  10% of sequence in fragments shorter than    1905 bp
  15% of sequence in fragments shorter than    2462 bp
  20% of sequence in fragments shorter than    2992 bp
  25% of sequence in fragments shorter than    3506 bp
  30% of sequence in fragments shorter than    4027 bp
  35% of sequence in fragments shorter than    4548 bp
  40% of sequence in fragments shorter than    5078 bp
  45% of sequence in fragments shorter than    5636 bp
  50% of sequence in fragments shorter than    6234 bp
  55% of sequence in fragments shorter than    6876 bp
  60% of sequence in fragments shorter than    7574 bp
  65% of sequence in fragments shorter than    8336 bp
  70% of sequence in fragments shorter than    9201 bp
  75% of sequence in fragments shorter than   10227 bp
  80% of sequence in fragments shorter than   11478 bp
  85% of sequence in fragments shorter than   13074 bp
  90% of sequence in fragments shorter than   15412 bp
  95% of sequence in fragments shorter than   19923 bp

# BciVI.SnaBI (GTATCC+TACGTA) double digest, 827 thousand fragments (696093+132299 sites), 3599 bp average fragment length
   5% of sequence in fragments shorter than    1290 bp
  10% of sequence in fragments shorter than    1907 bp
  15% of sequence in fragments shorter than    2464 bp
  20% of sequence in fragments shorter than    2994 bp
  25% of sequence in fragments shorter than    3509 bp
  30% of sequence in fragments shorter than    4025 bp
  35% of sequence in fragments shorter than    4545 bp
  40% of sequence in fragments shorter than    5083 bp
  45% of sequence in fragments shorter than    5648 bp
  50% of sequence in fragments shorter than    6250 bp
  55% of sequence in fragments shorter than    6892 bp
  60% of sequence in fragments shorter than    7597 bp
  65% of sequence in fragments shorter than    8387 bp
  70% of sequence in fragments shorter than    9282 bp
  75% of sequence in fragments shorter than   10334 bp
  80% of sequence in fragments shorter than   11602 bp
  85% of sequence in fragments shorter than   13260 bp
  90% of sequence in fragments shorter than   15729 bp
  95% of sequence in fragments shorter than   20355 bp

# AgeI.BglII (ACCGGT+AGATCT) double digest, 827 thousand fragments (53386+774732 sites), 3600 bp average fragment length
   5% of sequence in fragments shorter than    1250 bp
  10% of sequence in fragments shorter than    1879 bp
  15% of sequence in fragments shorter than    2436 bp
  20% of sequence in fragments shorter than    2963 bp
  25% of sequence in fragments shorter than    3481 bp
  30% of sequence in fragments shorter than    3993 bp
  35% of sequence in fragments shorter than    4508 bp
  40% of sequence in fragments shorter than    5035 bp
  45% of sequence in fragments shorter than    5587 bp
  50% of sequence in fragments shorter than    6177 bp
  55% of sequence in fragments shorter than    6815 bp
  60% of sequence in fragments shorter than    7514 bp
  65% of sequence in fragments shorter than    8279 bp
  70% of sequence in fragments shorter than    9154 bp
  75% of sequence in fragments shorter than   10190 bp
  80% of sequence in fragments shorter than   11426 bp
  85% of sequence in fragments shorter than   13091 bp
  90% of sequence in fragments shorter than   15487 bp
  95% of sequence in fragments shorter than   20349 bp

# NcoI.SacII (CCATGG+CCGCGG) double digest, 826 thousand fragments (759594+66901 sites), 3607 bp average fragment length
   5% of sequence in fragments shorter than    1252 bp
  10% of sequence in fragments shorter than    1941 bp
  15% of sequence in fragments shorter than    2556 bp
  20% of sequence in fragments shorter than    3135 bp
  25% of sequence in fragments shorter than    3710 bp
  30% of sequence in fragments shorter than    4282 bp
  35% of sequence in fragments shorter than    4844 bp
  40% of sequence in fragments shorter than    5448 bp
  45% of sequence in fragments shorter than    6058 bp
  50% of sequence in fragments shorter than    6726 bp
  55% of sequence in fragments shorter than    7462 bp
  60% of sequence in fragments shorter than    8269 bp
  65% of sequence in fragments shorter than    9169 bp
  70% of sequence in fragments shorter than   10201 bp
  75% of sequence in fragments shorter than   11414 bp
  80% of sequence in fragments shorter than   12898 bp
  85% of sequence in fragments shorter than   14843 bp
  90% of sequence in fragments shorter than   17735 bp
  95% of sequence in fragments shorter than   23490 bp

# BceAI.XhoI (ACGGC+CTCGAG) double digest, 826 thousand fragments (705178+121308 sites), 3607 bp average fragment length
   5% of sequence in fragments shorter than    1167 bp
  10% of sequence in fragments shorter than    1917 bp
  15% of sequence in fragments shorter than    2619 bp
  20% of sequence in fragments shorter than    3303 bp
  25% of sequence in fragments shorter than    3995 bp
  30% of sequence in fragments shorter than    4705 bp
  35% of sequence in fragments shorter than    5444 bp
  40% of sequence in fragments shorter than    6218 bp
  45% of sequence in fragments shorter than    7049 bp
  50% of sequence in fragments shorter than    7941 bp
  55% of sequence in fragments shorter than    8909 bp
  60% of sequence in fragments shorter than    9988 bp
  65% of sequence in fragments shorter than   11211 bp
  70% of sequence in fragments shorter than   12602 bp
  75% of sequence in fragments shorter than   14276 bp
  80% of sequence in fragments shorter than   16306 bp
  85% of sequence in fragments shorter than   18919 bp
  90% of sequence in fragments shorter than   22826 bp
  95% of sequence in fragments shorter than   30115 bp

# ApaI.BamHI (GGGCCC+GGATCC) double digest, 826 thousand fragments (460339+365999 sites), 3607 bp average fragment length
   5% of sequence in fragments shorter than    1168 bp
  10% of sequence in fragments shorter than    1913 bp
  15% of sequence in fragments shorter than    2594 bp
  20% of sequence in fragments shorter than    3276 bp
  25% of sequence in fragments shorter than    3974 bp
  30% of sequence in fragments shorter than    4676 bp
  35% of sequence in fragments shorter than    5402 bp
  40% of sequence in fragments shorter than    6177 bp
  45% of sequence in fragments shorter than    6997 bp
  50% of sequence in fragments shorter than    7878 bp
  55% of sequence in fragments shorter than    8823 bp
  60% of sequence in fragments shorter than    9881 bp
  65% of sequence in fragments shorter than   11062 bp
  70% of sequence in fragments shorter than   12440 bp
  75% of sequence in fragments shorter than   14088 bp
  80% of sequence in fragments shorter than   16117 bp
  85% of sequence in fragments shorter than   18761 bp
  90% of sequence in fragments shorter than   22675 bp
  95% of sequence in fragments shorter than   30754 bp

# AvrII.KasI (CCTAGG+GGCGCC) double digest, 824 thousand fragments (594956+230381 sites), 3612 bp average fragment length
   5% of sequence in fragments shorter than    1225 bp
  10% of sequence in fragments shorter than    1917 bp
  15% of sequence in fragments shorter than    2523 bp
  20% of sequence in fragments shorter than    3095 bp
  25% of sequence in fragments shorter than    3659 bp
  30% of sequence in fragments shorter than    4187 bp
  35% of sequence in fragments shorter than    4753 bp
  40% of sequence in fragments shorter than    5352 bp
  45% of sequence in fragments shorter than    5982 bp
  50% of sequence in fragments shorter than    6650 bp
  55% of sequence in fragments shorter than    7376 bp
  60% of sequence in fragments shorter than    8175 bp
  65% of sequence in fragments shorter than    9066 bp
  70% of sequence in fragments shorter than   10106 bp
  75% of sequence in fragments shorter than   11304 bp
  80% of sequence in fragments shorter than   12804 bp
  85% of sequence in fragments shorter than   14784 bp
  90% of sequence in fragments shorter than   17701 bp
  95% of sequence in fragments shorter than   23795 bp

# BclI.ClaI (TGATCA+ATCGAT) double digest, 824 thousand fragments (738785+86477 sites), 3612 bp average fragment length
   5% of sequence in fragments shorter than    1275 bp
  10% of sequence in fragments shorter than    1906 bp
  15% of sequence in fragments shorter than    2464 bp
  20% of sequence in fragments shorter than    2978 bp
  25% of sequence in fragments shorter than    3487 bp
  30% of sequence in fragments shorter than    3995 bp
  35% of sequence in fragments shorter than    4506 bp
  40% of sequence in fragments shorter than    5032 bp
  45% of sequence in fragments shorter than    5583 bp
  50% of sequence in fragments shorter than    6174 bp
  55% of sequence in fragments shorter than    6804 bp
  60% of sequence in fragments shorter than    7491 bp
  65% of sequence in fragments shorter than    8261 bp
  70% of sequence in fragments shorter than    9128 bp
  75% of sequence in fragments shorter than   10176 bp
  80% of sequence in fragments shorter than   11451 bp
  85% of sequence in fragments shorter than   13103 bp
  90% of sequence in fragments shorter than   15573 bp
  95% of sequence in fragments shorter than   20706 bp

# EcoRV.SapI (GATATC+GCTCTTC) double digest, 823 thousand fragments (446473+377161 sites), 3620 bp average fragment length
   5% of sequence in fragments shorter than    1236 bp
  10% of sequence in fragments shorter than    1903 bp
  15% of sequence in fragments shorter than    2478 bp
  20% of sequence in fragments shorter than    3021 bp
  25% of sequence in fragments shorter than    3552 bp
  30% of sequence in fragments shorter than    4083 bp
  35% of sequence in fragments shorter than    4617 bp
  40% of sequence in fragments shorter than    5165 bp
  45% of sequence in fragments shorter than    5736 bp
  50% of sequence in fragments shorter than    6337 bp
  55% of sequence in fragments shorter than    6995 bp
  60% of sequence in fragments shorter than    7708 bp
  65% of sequence in fragments shorter than    8501 bp
  70% of sequence in fragments shorter than    9397 bp
  75% of sequence in fragments shorter than   10443 bp
  80% of sequence in fragments shorter than   11704 bp
  85% of sequence in fragments shorter than   13364 bp
  90% of sequence in fragments shorter than   15727 bp
  95% of sequence in fragments shorter than   20103 bp

# EcoRV.SmaI (GATATC+CCCGGG) double digest, 822 thousand fragments (446473+376940 sites), 3620 bp average fragment length
   5% of sequence in fragments shorter than    1236 bp
  10% of sequence in fragments shorter than    1924 bp
  15% of sequence in fragments shorter than    2510 bp
  20% of sequence in fragments shorter than    3074 bp
  25% of sequence in fragments shorter than    3624 bp
  30% of sequence in fragments shorter than    4170 bp
  35% of sequence in fragments shorter than    4731 bp
  40% of sequence in fragments shorter than    5312 bp
  45% of sequence in fragments shorter than    5923 bp
  50% of sequence in fragments shorter than    6570 bp
  55% of sequence in fragments shorter than    7262 bp
  60% of sequence in fragments shorter than    8025 bp
  65% of sequence in fragments shorter than    8863 bp
  70% of sequence in fragments shorter than    9823 bp
  75% of sequence in fragments shorter than   10961 bp
  80% of sequence in fragments shorter than   12314 bp
  85% of sequence in fragments shorter than   14039 bp
  90% of sequence in fragments shorter than   16571 bp
  95% of sequence in fragments shorter than   21186 bp

# BciVI.NaeI (GTATCC+GCCGGC) double digest, 821 thousand fragments (696093+125692 sites), 3628 bp average fragment length
   5% of sequence in fragments shorter than    1295 bp
  10% of sequence in fragments shorter than    1940 bp
  15% of sequence in fragments shorter than    2508 bp
  20% of sequence in fragments shorter than    3058 bp
  25% of sequence in fragments shorter than    3587 bp
  30% of sequence in fragments shorter than    4110 bp
  35% of sequence in fragments shorter than    4644 bp
  40% of sequence in fragments shorter than    5193 bp
  45% of sequence in fragments shorter than    5762 bp
  50% of sequence in fragments shorter than    6367 bp
  55% of sequence in fragments shorter than    7015 bp
  60% of sequence in fragments shorter than    7727 bp
  65% of sequence in fragments shorter than    8511 bp
  70% of sequence in fragments shorter than    9393 bp
  75% of sequence in fragments shorter than   10427 bp
  80% of sequence in fragments shorter than   11676 bp
  85% of sequence in fragments shorter than   13311 bp
  90% of sequence in fragments shorter than   15614 bp
  95% of sequence in fragments shorter than   19843 bp

# AvrII.SwaI (CCTAGG+ATTTAAAT) double digest, 821 thousand fragments (594956+226602 sites), 3628 bp average fragment length
   5% of sequence in fragments shorter than    1274 bp
  10% of sequence in fragments shorter than    1939 bp
  15% of sequence in fragments shorter than    2506 bp
  20% of sequence in fragments shorter than    3038 bp
  25% of sequence in fragments shorter than    3556 bp
  30% of sequence in fragments shorter than    4067 bp
  35% of sequence in fragments shorter than    4584 bp
  40% of sequence in fragments shorter than    5109 bp
  45% of sequence in fragments shorter than    5660 bp
  50% of sequence in fragments shorter than    6243 bp
  55% of sequence in fragments shorter than    6874 bp
  60% of sequence in fragments shorter than    7553 bp
  65% of sequence in fragments shorter than    8308 bp
  70% of sequence in fragments shorter than    9162 bp
  75% of sequence in fragments shorter than   10143 bp
  80% of sequence in fragments shorter than   11369 bp
  85% of sequence in fragments shorter than   12951 bp
  90% of sequence in fragments shorter than   15304 bp
  95% of sequence in fragments shorter than   20076 bp

# ClaI.FnuDII (ATCGAT+CGCG) double digest, 820 thousand fragments (86477+733940 sites), 3634 bp average fragment length
   5% of sequence in fragments shorter than    1596 bp
  10% of sequence in fragments shorter than    2759 bp
  15% of sequence in fragments shorter than    3819 bp
  20% of sequence in fragments shorter than    4887 bp
  25% of sequence in fragments shorter than    5963 bp
  30% of sequence in fragments shorter than    7087 bp
  35% of sequence in fragments shorter than    8271 bp
  40% of sequence in fragments shorter than    9522 bp
  45% of sequence in fragments shorter than   10873 bp
  50% of sequence in fragments shorter than   12361 bp
  55% of sequence in fragments shorter than   14010 bp
  60% of sequence in fragments shorter than   15832 bp
  65% of sequence in fragments shorter than   17900 bp
  70% of sequence in fragments shorter than   20311 bp
  75% of sequence in fragments shorter than   23115 bp
  80% of sequence in fragments shorter than   26584 bp
  85% of sequence in fragments shorter than   31182 bp
  90% of sequence in fragments shorter than   38083 bp
  95% of sequence in fragments shorter than   50809 bp

# NcoI.PspXI (CCATGG+VCTCGAGB) double digest, 818 thousand fragments (759594+59193 sites), 3641 bp average fragment length
   5% of sequence in fragments shorter than    1258 bp
  10% of sequence in fragments shorter than    1926 bp
  15% of sequence in fragments shorter than    2522 bp
  20% of sequence in fragments shorter than    3086 bp
  25% of sequence in fragments shorter than    3643 bp
  30% of sequence in fragments shorter than    4197 bp
  35% of sequence in fragments shorter than    4743 bp
  40% of sequence in fragments shorter than    5331 bp
  45% of sequence in fragments shorter than    5923 bp
  50% of sequence in fragments shorter than    6568 bp
  55% of sequence in fragments shorter than    7283 bp
  60% of sequence in fragments shorter than    8073 bp
  65% of sequence in fragments shorter than    8949 bp
  70% of sequence in fragments shorter than    9948 bp
  75% of sequence in fragments shorter than   11123 bp
  80% of sequence in fragments shorter than   12564 bp
  85% of sequence in fragments shorter than   14451 bp
  90% of sequence in fragments shorter than   17234 bp
  95% of sequence in fragments shorter than   22806 bp

# BciVI.XhoI (GTATCC+CTCGAG) double digest, 816 thousand fragments (696093+121308 sites), 3647 bp average fragment length
   5% of sequence in fragments shorter than    1292 bp
  10% of sequence in fragments shorter than    1922 bp
  15% of sequence in fragments shorter than    2481 bp
  20% of sequence in fragments shorter than    3004 bp
  25% of sequence in fragments shorter than    3519 bp
  30% of sequence in fragments shorter than    4034 bp
  35% of sequence in fragments shorter than    4554 bp
  40% of sequence in fragments shorter than    5094 bp
  45% of sequence in fragments shorter than    5659 bp
  50% of sequence in fragments shorter than    6258 bp
  55% of sequence in fragments shorter than    6892 bp
  60% of sequence in fragments shorter than    7586 bp
  65% of sequence in fragments shorter than    8355 bp
  70% of sequence in fragments shorter than    9226 bp
  75% of sequence in fragments shorter than   10245 bp
  80% of sequence in fragments shorter than   11457 bp
  85% of sequence in fragments shorter than   13038 bp
  90% of sequence in fragments shorter than   15260 bp
  95% of sequence in fragments shorter than   19412 bp

# BglII.PmeI (AGATCT+GTTTAAAC) double digest, 816 thousand fragments (774732+42094 sites), 3650 bp average fragment length
   5% of sequence in fragments shorter than    1273 bp
  10% of sequence in fragments shorter than    1896 bp
  15% of sequence in fragments shorter than    2458 bp
  20% of sequence in fragments shorter than    2993 bp
  25% of sequence in fragments shorter than    3516 bp
  30% of sequence in fragments shorter than    4033 bp
  35% of sequence in fragments shorter than    4553 bp
  40% of sequence in fragments shorter than    5088 bp
  45% of sequence in fragments shorter than    5652 bp
  50% of sequence in fragments shorter than    6252 bp
  55% of sequence in fragments shorter than    6897 bp
  60% of sequence in fragments shorter than    7613 bp
  65% of sequence in fragments shorter than    8401 bp
  70% of sequence in fragments shorter than    9302 bp
  75% of sequence in fragments shorter than   10361 bp
  80% of sequence in fragments shorter than   11653 bp
  85% of sequence in fragments shorter than   13375 bp
  90% of sequence in fragments shorter than   15890 bp
  95% of sequence in fragments shorter than   21059 bp

# AclI.AflII (AACGTT+CTTAAG) double digest, 815 thousand fragments (172706+643284 sites), 3653 bp average fragment length
   5% of sequence in fragments shorter than    1265 bp
  10% of sequence in fragments shorter than    1926 bp
  15% of sequence in fragments shorter than    2482 bp
  20% of sequence in fragments shorter than    3000 bp
  25% of sequence in fragments shorter than    3516 bp
  30% of sequence in fragments shorter than    4032 bp
  35% of sequence in fragments shorter than    4558 bp
  40% of sequence in fragments shorter than    5097 bp
  45% of sequence in fragments shorter than    5662 bp
  50% of sequence in fragments shorter than    6268 bp
  55% of sequence in fragments shorter than    6917 bp
  60% of sequence in fragments shorter than    7630 bp
  65% of sequence in fragments shorter than    8419 bp
  70% of sequence in fragments shorter than    9325 bp
  75% of sequence in fragments shorter than   10404 bp
  80% of sequence in fragments shorter than   11707 bp
  85% of sequence in fragments shorter than   13430 bp
  90% of sequence in fragments shorter than   15983 bp
  95% of sequence in fragments shorter than   21111 bp

# AgeI.NcoI (ACCGGT+CCATGG) double digest, 812 thousand fragments (53386+759594 sites), 3667 bp average fragment length
   5% of sequence in fragments shorter than    1272 bp
  10% of sequence in fragments shorter than    1944 bp
  15% of sequence in fragments shorter than    2542 bp
  20% of sequence in fragments shorter than    3106 bp
  25% of sequence in fragments shorter than    3663 bp
  30% of sequence in fragments shorter than    4182 bp
  35% of sequence in fragments shorter than    4735 bp
  40% of sequence in fragments shorter than    5323 bp
  45% of sequence in fragments shorter than    5922 bp
  50% of sequence in fragments shorter than    6571 bp
  55% of sequence in fragments shorter than    7292 bp
  60% of sequence in fragments shorter than    8084 bp
  65% of sequence in fragments shorter than    8968 bp
  70% of sequence in fragments shorter than    9976 bp
  75% of sequence in fragments shorter than   11158 bp
  80% of sequence in fragments shorter than   12594 bp
  85% of sequence in fragments shorter than   14496 bp
  90% of sequence in fragments shorter than   17267 bp
  95% of sequence in fragments shorter than   22824 bp

# BamHI.EcoRV (GGATCC+GATATC) double digest, 812 thousand fragments (365999+446473 sites), 3669 bp average fragment length
   5% of sequence in fragments shorter than    1294 bp
  10% of sequence in fragments shorter than    1916 bp
  15% of sequence in fragments shorter than    2480 bp
  20% of sequence in fragments shorter than    3026 bp
  25% of sequence in fragments shorter than    3554 bp
  30% of sequence in fragments shorter than    4081 bp
  35% of sequence in fragments shorter than    4614 bp
  40% of sequence in fragments shorter than    5163 bp
  45% of sequence in fragments shorter than    5733 bp
  50% of sequence in fragments shorter than    6342 bp
  55% of sequence in fragments shorter than    6992 bp
  60% of sequence in fragments shorter than    7711 bp
  65% of sequence in fragments shorter than    8483 bp
  70% of sequence in fragments shorter than    9363 bp
  75% of sequence in fragments shorter than   10388 bp
  80% of sequence in fragments shorter than   11639 bp
  85% of sequence in fragments shorter than   13230 bp
  90% of sequence in fragments shorter than   15497 bp
  95% of sequence in fragments shorter than   19556 bp

# AatII.BclI (GACGTC+TGATCA) double digest, 809 thousand fragments (71089+738785 sites), 3681 bp average fragment length
   5% of sequence in fragments shorter than    1304 bp
  10% of sequence in fragments shorter than    1950 bp
  15% of sequence in fragments shorter than    2510 bp
  20% of sequence in fragments shorter than    3028 bp
  25% of sequence in fragments shorter than    3545 bp
  30% of sequence in fragments shorter than    4056 bp
  35% of sequence in fragments shorter than    4573 bp
  40% of sequence in fragments shorter than    5099 bp
  45% of sequence in fragments shorter than    5655 bp
  50% of sequence in fragments shorter than    6243 bp
  55% of sequence in fragments shorter than    6878 bp
  60% of sequence in fragments shorter than    7560 bp
  65% of sequence in fragments shorter than    8328 bp
  70% of sequence in fragments shorter than    9187 bp
  75% of sequence in fragments shorter than   10215 bp
  80% of sequence in fragments shorter than   11461 bp
  85% of sequence in fragments shorter than   13069 bp
  90% of sequence in fragments shorter than   15406 bp
  95% of sequence in fragments shorter than   20047 bp

# BsrBI.HgaI (CCGCTC+GACGC) double digest, 806 thousand fragments (235523+571889 sites), 3692 bp average fragment length
   5% of sequence in fragments shorter than    1228 bp
  10% of sequence in fragments shorter than    2100 bp
  15% of sequence in fragments shorter than    2917 bp
  20% of sequence in fragments shorter than    3729 bp
  25% of sequence in fragments shorter than    4573 bp
  30% of sequence in fragments shorter than    5442 bp
  35% of sequence in fragments shorter than    6348 bp
  40% of sequence in fragments shorter than    7299 bp
  45% of sequence in fragments shorter than    8328 bp
  50% of sequence in fragments shorter than    9432 bp
  55% of sequence in fragments shorter than   10654 bp
  60% of sequence in fragments shorter than   12002 bp
  65% of sequence in fragments shorter than   13553 bp
  70% of sequence in fragments shorter than   15308 bp
  75% of sequence in fragments shorter than   17371 bp
  80% of sequence in fragments shorter than   19949 bp
  85% of sequence in fragments shorter than   23231 bp
  90% of sequence in fragments shorter than   28058 bp
  95% of sequence in fragments shorter than   36909 bp

# BsrBI.EciI (CCGCTC+GGCGGA) double digest, 806 thousand fragments (235523+571273 sites), 3695 bp average fragment length
   5% of sequence in fragments shorter than    1190 bp
  10% of sequence in fragments shorter than    1989 bp
  15% of sequence in fragments shorter than    2741 bp
  20% of sequence in fragments shorter than    3499 bp
  25% of sequence in fragments shorter than    4292 bp
  30% of sequence in fragments shorter than    5138 bp
  35% of sequence in fragments shorter than    6040 bp
  40% of sequence in fragments shorter than    7017 bp
  45% of sequence in fragments shorter than    8089 bp
  50% of sequence in fragments shorter than    9271 bp
  55% of sequence in fragments shorter than   10616 bp
  60% of sequence in fragments shorter than   12122 bp
  65% of sequence in fragments shorter than   13840 bp
  70% of sequence in fragments shorter than   15878 bp
  75% of sequence in fragments shorter than   18254 bp
  80% of sequence in fragments shorter than   21305 bp
  85% of sequence in fragments shorter than   25278 bp
  90% of sequence in fragments shorter than   31093 bp
  95% of sequence in fragments shorter than   42102 bp

# BglII.SalI (AGATCT+GTCGAC) double digest, 805 thousand fragments (774732+31396 sites), 3698 bp average fragment length
   5% of sequence in fragments shorter than    1289 bp
  10% of sequence in fragments shorter than    1923 bp
  15% of sequence in fragments shorter than    2493 bp
  20% of sequence in fragments shorter than    3035 bp
  25% of sequence in fragments shorter than    3565 bp
  30% of sequence in fragments shorter than    4089 bp
  35% of sequence in fragments shorter than    4617 bp
  40% of sequence in fragments shorter than    5159 bp
  45% of sequence in fragments shorter than    5727 bp
  50% of sequence in fragments shorter than    6331 bp
  55% of sequence in fragments shorter than    6984 bp
  60% of sequence in fragments shorter than    7703 bp
  65% of sequence in fragments shorter than    8496 bp
  70% of sequence in fragments shorter than    9405 bp
  75% of sequence in fragments shorter than   10468 bp
  80% of sequence in fragments shorter than   11751 bp
  85% of sequence in fragments shorter than   13468 bp
  90% of sequence in fragments shorter than   15923 bp
  95% of sequence in fragments shorter than   21025 bp

# BclI.SacII (TGATCA+CCGCGG) double digest, 805 thousand fragments (738785+66901 sites), 3700 bp average fragment length
   5% of sequence in fragments shorter than    1319 bp
  10% of sequence in fragments shorter than    1991 bp
  15% of sequence in fragments shorter than    2570 bp
  20% of sequence in fragments shorter than    3107 bp
  25% of sequence in fragments shorter than    3642 bp
  30% of sequence in fragments shorter than    4165 bp
  35% of sequence in fragments shorter than    4695 bp
  40% of sequence in fragments shorter than    5241 bp
  45% of sequence in fragments shorter than    5811 bp
  50% of sequence in fragments shorter than    6420 bp
  55% of sequence in fragments shorter than    7066 bp
  60% of sequence in fragments shorter than    7772 bp
  65% of sequence in fragments shorter than    8554 bp
  70% of sequence in fragments shorter than    9440 bp
  75% of sequence in fragments shorter than   10502 bp
  80% of sequence in fragments shorter than   11772 bp
  85% of sequence in fragments shorter than   13421 bp
  90% of sequence in fragments shorter than   15846 bp
  95% of sequence in fragments shorter than   20792 bp

# AatII.FnuDII (GACGTC+CGCG) double digest, 804 thousand fragments (71089+733940 sites), 3703 bp average fragment length
   5% of sequence in fragments shorter than    1606 bp
  10% of sequence in fragments shorter than    2827 bp
  15% of sequence in fragments shorter than    3984 bp
  20% of sequence in fragments shorter than    5162 bp
  25% of sequence in fragments shorter than    6373 bp
  30% of sequence in fragments shorter than    7653 bp
  35% of sequence in fragments shorter than    9031 bp
  40% of sequence in fragments shorter than   10503 bp
  45% of sequence in fragments shorter than   12123 bp
  50% of sequence in fragments shorter than   13915 bp
  55% of sequence in fragments shorter than   15896 bp
  60% of sequence in fragments shorter than   18140 bp
  65% of sequence in fragments shorter than   20713 bp
  70% of sequence in fragments shorter than   23670 bp
  75% of sequence in fragments shorter than   27170 bp
  80% of sequence in fragments shorter than   31511 bp
  85% of sequence in fragments shorter than   37297 bp
  90% of sequence in fragments shorter than   46047 bp
  95% of sequence in fragments shorter than   62684 bp

# AflII.PacI (CTTAAG+TTAATTAA) double digest, 802 thousand fragments (643284+159455 sites), 3713 bp average fragment length
   5% of sequence in fragments shorter than    1312 bp
  10% of sequence in fragments shorter than    1988 bp
  15% of sequence in fragments shorter than    2558 bp
  20% of sequence in fragments shorter than    3095 bp
  25% of sequence in fragments shorter than    3624 bp
  30% of sequence in fragments shorter than    4156 bp
  35% of sequence in fragments shorter than    4693 bp
  40% of sequence in fragments shorter than    5248 bp
  45% of sequence in fragments shorter than    5834 bp
  50% of sequence in fragments shorter than    6462 bp
  55% of sequence in fragments shorter than    7150 bp
  60% of sequence in fragments shorter than    7902 bp
  65% of sequence in fragments shorter than    8738 bp
  70% of sequence in fragments shorter than    9715 bp
  75% of sequence in fragments shorter than   10888 bp
  80% of sequence in fragments shorter than   12333 bp
  85% of sequence in fragments shorter than   14274 bp
  90% of sequence in fragments shorter than   17312 bp
  95% of sequence in fragments shorter than   24403 bp

# AclI.HaeII (AACGTT+RGCGCY) double digest, 802 thousand fragments (172706+630101 sites), 3714 bp average fragment length
   5% of sequence in fragments shorter than    1139 bp
  10% of sequence in fragments shorter than    2059 bp
  15% of sequence in fragments shorter than    2869 bp
  20% of sequence in fragments shorter than    3641 bp
  25% of sequence in fragments shorter than    4432 bp
  30% of sequence in fragments shorter than    5227 bp
  35% of sequence in fragments shorter than    6056 bp
  40% of sequence in fragments shorter than    6926 bp
  45% of sequence in fragments shorter than    7854 bp
  50% of sequence in fragments shorter than    8840 bp
  55% of sequence in fragments shorter than    9925 bp
  60% of sequence in fragments shorter than   11099 bp
  65% of sequence in fragments shorter than   12427 bp
  70% of sequence in fragments shorter than   13939 bp
  75% of sequence in fragments shorter than   15729 bp
  80% of sequence in fragments shorter than   17898 bp
  85% of sequence in fragments shorter than   20702 bp
  90% of sequence in fragments shorter than   24624 bp
  95% of sequence in fragments shorter than   31290 bp

# HgaI.KasI (GACGC+GGCGCC) double digest, 801 thousand fragments (571889+230381 sites), 3716 bp average fragment length
   5% of sequence in fragments shorter than    1238 bp
  10% of sequence in fragments shorter than    2086 bp
  15% of sequence in fragments shorter than    2869 bp
  20% of sequence in fragments shorter than    3647 bp
  25% of sequence in fragments shorter than    4453 bp
  30% of sequence in fragments shorter than    5286 bp
  35% of sequence in fragments shorter than    6158 bp
  40% of sequence in fragments shorter than    7077 bp
  45% of sequence in fragments shorter than    8068 bp
  50% of sequence in fragments shorter than    9163 bp
  55% of sequence in fragments shorter than   10354 bp
  60% of sequence in fragments shorter than   11692 bp
  65% of sequence in fragments shorter than   13214 bp
  70% of sequence in fragments shorter than   14957 bp
  75% of sequence in fragments shorter than   17044 bp
  80% of sequence in fragments shorter than   19616 bp
  85% of sequence in fragments shorter than   22985 bp
  90% of sequence in fragments shorter than   27952 bp
  95% of sequence in fragments shorter than   37539 bp

# EciI.KasI (GGCGGA+GGCGCC) double digest, 801 thousand fragments (571273+230381 sites), 3718 bp average fragment length
   5% of sequence in fragments shorter than    1266 bp
  10% of sequence in fragments shorter than    2144 bp
  15% of sequence in fragments shorter than    2966 bp
  20% of sequence in fragments shorter than    3819 bp
  25% of sequence in fragments shorter than    4721 bp
  30% of sequence in fragments shorter than    5682 bp
  35% of sequence in fragments shorter than    6712 bp
  40% of sequence in fragments shorter than    7830 bp
  45% of sequence in fragments shorter than    9048 bp
  50% of sequence in fragments shorter than   10419 bp
  55% of sequence in fragments shorter than   11939 bp
  60% of sequence in fragments shorter than   13684 bp
  65% of sequence in fragments shorter than   15690 bp
  70% of sequence in fragments shorter than   18020 bp
  75% of sequence in fragments shorter than   20787 bp
  80% of sequence in fragments shorter than   24331 bp
  85% of sequence in fragments shorter than   28914 bp
  90% of sequence in fragments shorter than   35967 bp
  95% of sequence in fragments shorter than   50287 bp

# NcoI.PmeI (CCATGG+GTTTAAAC) double digest, 801 thousand fragments (759594+42094 sites), 3718 bp average fragment length
   5% of sequence in fragments shorter than    1293 bp
  10% of sequence in fragments shorter than    1971 bp
  15% of sequence in fragments shorter than    2574 bp
  20% of sequence in fragments shorter than    3142 bp
  25% of sequence in fragments shorter than    3703 bp
  30% of sequence in fragments shorter than    4259 bp
  35% of sequence in fragments shorter than    4804 bp
  40% of sequence in fragments shorter than    5391 bp
  45% of sequence in fragments shorter than    5981 bp
  50% of sequence in fragments shorter than    6627 bp
  55% of sequence in fragments shorter than    7340 bp
  60% of sequence in fragments shorter than    8117 bp
  65% of sequence in fragments shorter than    8980 bp
  70% of sequence in fragments shorter than    9967 bp
  75% of sequence in fragments shorter than   11125 bp
  80% of sequence in fragments shorter than   12544 bp
  85% of sequence in fragments shorter than   14378 bp
  90% of sequence in fragments shorter than   17075 bp
  95% of sequence in fragments shorter than   22496 bp

# AflII.Hpy99I (CTTAAG+CGWCG) double digest, 800 thousand fragments (643284+157917 sites), 3721 bp average fragment length
   5% of sequence in fragments shorter than    1325 bp
  10% of sequence in fragments shorter than    2019 bp
  15% of sequence in fragments shorter than    2609 bp
  20% of sequence in fragments shorter than    3158 bp
  25% of sequence in fragments shorter than    3697 bp
  30% of sequence in fragments shorter than    4217 bp
  35% of sequence in fragments shorter than    4753 bp
  40% of sequence in fragments shorter than    5314 bp
  45% of sequence in fragments shorter than    5898 bp
  50% of sequence in fragments shorter than    6521 bp
  55% of sequence in fragments shorter than    7186 bp
  60% of sequence in fragments shorter than    7916 bp
  65% of sequence in fragments shorter than    8713 bp
  70% of sequence in fragments shorter than    9629 bp
  75% of sequence in fragments shorter than   10700 bp
  80% of sequence in fragments shorter than   11973 bp
  85% of sequence in fragments shorter than   13647 bp
  90% of sequence in fragments shorter than   16107 bp
  95% of sequence in fragments shorter than   21003 bp

# FnuDII.SacII (CGCG+CCGCGG) double digest, 800 thousand fragments (733940+66901 sites), 3722 bp average fragment length
   5% of sequence in fragments shorter than    1828 bp
  10% of sequence in fragments shorter than    3245 bp
  15% of sequence in fragments shorter than    4614 bp
  20% of sequence in fragments shorter than    6000 bp
  25% of sequence in fragments shorter than    7450 bp
  30% of sequence in fragments shorter than    8999 bp
  35% of sequence in fragments shorter than   10672 bp
  40% of sequence in fragments shorter than   12472 bp
  45% of sequence in fragments shorter than   14502 bp
  50% of sequence in fragments shorter than   16747 bp
  55% of sequence in fragments shorter than   19236 bp
  60% of sequence in fragments shorter than   22038 bp
  65% of sequence in fragments shorter than   25222 bp
  70% of sequence in fragments shorter than   28949 bp
  75% of sequence in fragments shorter than   33421 bp
  80% of sequence in fragments shorter than   39112 bp
  85% of sequence in fragments shorter than   46701 bp
  90% of sequence in fragments shorter than   57740 bp
  95% of sequence in fragments shorter than   79981 bp

# BsaAI.ClaI (YACGTR+ATCGAT) double digest, 799 thousand fragments (713801+86477 sites), 3725 bp average fragment length
   5% of sequence in fragments shorter than    1325 bp
  10% of sequence in fragments shorter than    1990 bp
  15% of sequence in fragments shorter than    2585 bp
  20% of sequence in fragments shorter than    3145 bp
  25% of sequence in fragments shorter than    3673 bp
  30% of sequence in fragments shorter than    4231 bp
  35% of sequence in fragments shorter than    4785 bp
  40% of sequence in fragments shorter than    5372 bp
  45% of sequence in fragments shorter than    5983 bp
  50% of sequence in fragments shorter than    6633 bp
  55% of sequence in fragments shorter than    7323 bp
  60% of sequence in fragments shorter than    8084 bp
  65% of sequence in fragments shorter than    8917 bp
  70% of sequence in fragments shorter than    9876 bp
  75% of sequence in fragments shorter than   10980 bp
  80% of sequence in fragments shorter than   12309 bp
  85% of sequence in fragments shorter than   14045 bp
  90% of sequence in fragments shorter than   16480 bp
  95% of sequence in fragments shorter than   20933 bp

# BsrBI.MfeI (CCGCTC+CAATTG) double digest, 799 thousand fragments (235523+564303 sites), 3728 bp average fragment length
   5% of sequence in fragments shorter than    1311 bp
  10% of sequence in fragments shorter than    1991 bp
  15% of sequence in fragments shorter than    2587 bp
  20% of sequence in fragments shorter than    3147 bp
  25% of sequence in fragments shorter than    3699 bp
  30% of sequence in fragments shorter than    4246 bp
  35% of sequence in fragments shorter than    4797 bp
  40% of sequence in fragments shorter than    5368 bp
  45% of sequence in fragments shorter than    5961 bp
  50% of sequence in fragments shorter than    6585 bp
  55% of sequence in fragments shorter than    7258 bp
  60% of sequence in fragments shorter than    7995 bp
  65% of sequence in fragments shorter than    8820 bp
  70% of sequence in fragments shorter than    9742 bp
  75% of sequence in fragments shorter than   10824 bp
  80% of sequence in fragments shorter than   12109 bp
  85% of sequence in fragments shorter than   13767 bp
  90% of sequence in fragments shorter than   16130 bp
  95% of sequence in fragments shorter than   20520 bp

# HgaI.SwaI (GACGC+ATTTAAAT) double digest, 798 thousand fragments (571889+226602 sites), 3733 bp average fragment length
   5% of sequence in fragments shorter than    1297 bp
  10% of sequence in fragments shorter than    2069 bp
  15% of sequence in fragments shorter than    2756 bp
  20% of sequence in fragments shorter than    3389 bp
  25% of sequence in fragments shorter than    4027 bp
  30% of sequence in fragments shorter than    4673 bp
  35% of sequence in fragments shorter than    5332 bp
  40% of sequence in fragments shorter than    6013 bp
  45% of sequence in fragments shorter than    6731 bp
  50% of sequence in fragments shorter than    7499 bp
  55% of sequence in fragments shorter than    8324 bp
  60% of sequence in fragments shorter than    9230 bp
  65% of sequence in fragments shorter than   10224 bp
  70% of sequence in fragments shorter than   11373 bp
  75% of sequence in fragments shorter than   12725 bp
  80% of sequence in fragments shorter than   14351 bp
  85% of sequence in fragments shorter than   16489 bp
  90% of sequence in fragments shorter than   19521 bp
  95% of sequence in fragments shorter than   25396 bp

# BclI.PspXI (TGATCA+VCTCGAGB) double digest, 797 thousand fragments (738785+59193 sites), 3736 bp average fragment length
   5% of sequence in fragments shorter than    1323 bp
  10% of sequence in fragments shorter than    1980 bp
  15% of sequence in fragments shorter than    2549 bp
  20% of sequence in fragments shorter than    3076 bp
  25% of sequence in fragments shorter than    3599 bp
  30% of sequence in fragments shorter than    4115 bp
  35% of sequence in fragments shorter than    4639 bp
  40% of sequence in fragments shorter than    5171 bp
  45% of sequence in fragments shorter than    5735 bp
  50% of sequence in fragments shorter than    6333 bp
  55% of sequence in fragments shorter than    6974 bp
  60% of sequence in fragments shorter than    7667 bp
  65% of sequence in fragments shorter than    8443 bp
  70% of sequence in fragments shorter than    9312 bp
  75% of sequence in fragments shorter than   10354 bp
  80% of sequence in fragments shorter than   11606 bp
  85% of sequence in fragments shorter than   13240 bp
  90% of sequence in fragments shorter than   15628 bp
  95% of sequence in fragments shorter than   20531 bp

# EciI.SwaI (GGCGGA+ATTTAAAT) double digest, 797 thousand fragments (571273+226602 sites), 3736 bp average fragment length
   5% of sequence in fragments shorter than    1259 bp
  10% of sequence in fragments shorter than    2003 bp
  15% of sequence in fragments shorter than    2666 bp
  20% of sequence in fragments shorter than    3298 bp
  25% of sequence in fragments shorter than    3936 bp
  30% of sequence in fragments shorter than    4594 bp
  35% of sequence in fragments shorter than    5278 bp
  40% of sequence in fragments shorter than    5999 bp
  45% of sequence in fragments shorter than    6769 bp
  50% of sequence in fragments shorter than    7594 bp
  55% of sequence in fragments shorter than    8489 bp
  60% of sequence in fragments shorter than    9477 bp
  65% of sequence in fragments shorter than   10610 bp
  70% of sequence in fragments shorter than   11917 bp
  75% of sequence in fragments shorter than   13458 bp
  80% of sequence in fragments shorter than   15388 bp
  85% of sequence in fragments shorter than   17959 bp
  90% of sequence in fragments shorter than   21736 bp
  95% of sequence in fragments shorter than   29845 bp

# ApaI.BstEII (GGGCCC+GGTNACC) double digest, 795 thousand fragments (460339+335865 sites), 3744 bp average fragment length
   5% of sequence in fragments shorter than    1193 bp
  10% of sequence in fragments shorter than    1981 bp
  15% of sequence in fragments shorter than    2713 bp
  20% of sequence in fragments shorter than    3437 bp
  25% of sequence in fragments shorter than    4166 bp
  30% of sequence in fragments shorter than    4913 bp
  35% of sequence in fragments shorter than    5677 bp
  40% of sequence in fragments shorter than    6488 bp
  45% of sequence in fragments shorter than    7351 bp
  50% of sequence in fragments shorter than    8268 bp
  55% of sequence in fragments shorter than    9259 bp
  60% of sequence in fragments shorter than   10365 bp
  65% of sequence in fragments shorter than   11642 bp
  70% of sequence in fragments shorter than   13106 bp
  75% of sequence in fragments shorter than   14818 bp
  80% of sequence in fragments shorter than   16928 bp
  85% of sequence in fragments shorter than   19805 bp
  90% of sequence in fragments shorter than   23950 bp
  95% of sequence in fragments shorter than   33210 bp

# BglII.MluI (AGATCT+ACGCGT) double digest, 795 thousand fragments (774732+21415 sites), 3744 bp average fragment length
   5% of sequence in fragments shorter than    1307 bp
  10% of sequence in fragments shorter than    1948 bp
  15% of sequence in fragments shorter than    2528 bp
  20% of sequence in fragments shorter than    3078 bp
  25% of sequence in fragments shorter than    3612 bp
  30% of sequence in fragments shorter than    4140 bp
  35% of sequence in fragments shorter than    4673 bp
  40% of sequence in fragments shorter than    5219 bp
  45% of sequence in fragments shorter than    5795 bp
  50% of sequence in fragments shorter than    6408 bp
  55% of sequence in fragments shorter than    7067 bp
  60% of sequence in fragments shorter than    7792 bp
  65% of sequence in fragments shorter than    8594 bp
  70% of sequence in fragments shorter than    9508 bp
  75% of sequence in fragments shorter than   10570 bp
  80% of sequence in fragments shorter than   11865 bp
  85% of sequence in fragments shorter than   13604 bp
  90% of sequence in fragments shorter than   16077 bp
  95% of sequence in fragments shorter than   21211 bp

# KasI.MfeI (GGCGCC+CAATTG) double digest, 794 thousand fragments (230381+564303 sites), 3751 bp average fragment length
   5% of sequence in fragments shorter than    1315 bp
  10% of sequence in fragments shorter than    2009 bp
  15% of sequence in fragments shorter than    2592 bp
  20% of sequence in fragments shorter than    3146 bp
  25% of sequence in fragments shorter than    3691 bp
  30% of sequence in fragments shorter than    4197 bp
  35% of sequence in fragments shorter than    4740 bp
  40% of sequence in fragments shorter than    5303 bp
  45% of sequence in fragments shorter than    5887 bp
  50% of sequence in fragments shorter than    6508 bp
  55% of sequence in fragments shorter than    7167 bp
  60% of sequence in fragments shorter than    7902 bp
  65% of sequence in fragments shorter than    8713 bp
  70% of sequence in fragments shorter than    9631 bp
  75% of sequence in fragments shorter than   10706 bp
  80% of sequence in fragments shorter than   11981 bp
  85% of sequence in fragments shorter than   13636 bp
  90% of sequence in fragments shorter than   16010 bp
  95% of sequence in fragments shorter than   20510 bp

# ApaLI.SexAI (GTGCAC+ACCWGGT) double digest, 793 thousand fragments (496255+297900 sites), 3754 bp average fragment length
   5% of sequence in fragments shorter than    1313 bp
  10% of sequence in fragments shorter than    1999 bp
  15% of sequence in fragments shorter than    2596 bp
  20% of sequence in fragments shorter than    3161 bp
  25% of sequence in fragments shorter than    3710 bp
  30% of sequence in fragments shorter than    4267 bp
  35% of sequence in fragments shorter than    4828 bp
  40% of sequence in fragments shorter than    5421 bp
  45% of sequence in fragments shorter than    6017 bp
  50% of sequence in fragments shorter than    6649 bp
  55% of sequence in fragments shorter than    7344 bp
  60% of sequence in fragments shorter than    8115 bp
  65% of sequence in fragments shorter than    8973 bp
  70% of sequence in fragments shorter than    9939 bp
  75% of sequence in fragments shorter than   11098 bp
  80% of sequence in fragments shorter than   12530 bp
  85% of sequence in fragments shorter than   14373 bp
  90% of sequence in fragments shorter than   17061 bp
  95% of sequence in fragments shorter than   22297 bp

# FnuDII.PspXI (CGCG+VCTCGAGB) double digest, 792 thousand fragments (733940+59193 sites), 3759 bp average fragment length
   5% of sequence in fragments shorter than    1631 bp
  10% of sequence in fragments shorter than    2880 bp
  15% of sequence in fragments shorter than    4068 bp
  20% of sequence in fragments shorter than    5285 bp
  25% of sequence in fragments shorter than    6538 bp
  30% of sequence in fragments shorter than    7871 bp
  35% of sequence in fragments shorter than    9308 bp
  40% of sequence in fragments shorter than   10847 bp
  45% of sequence in fragments shorter than   12538 bp
  50% of sequence in fragments shorter than   14420 bp
  55% of sequence in fragments shorter than   16522 bp
  60% of sequence in fragments shorter than   18904 bp
  65% of sequence in fragments shorter than   21610 bp
  70% of sequence in fragments shorter than   24677 bp
  75% of sequence in fragments shorter than   28418 bp
  80% of sequence in fragments shorter than   33176 bp
  85% of sequence in fragments shorter than   39526 bp
  90% of sequence in fragments shorter than   49012 bp
  95% of sequence in fragments shorter than   66608 bp

# AgeI.BclI (ACCGGT+TGATCA) double digest, 791 thousand fragments (53386+738785 sites), 3763 bp average fragment length
   5% of sequence in fragments shorter than    1330 bp
  10% of sequence in fragments shorter than    1975 bp
  15% of sequence in fragments shorter than    2552 bp
  20% of sequence in fragments shorter than    3090 bp
  25% of sequence in fragments shorter than    3618 bp
  30% of sequence in fragments shorter than    4142 bp
  35% of sequence in fragments shorter than    4673 bp
  40% of sequence in fragments shorter than    5216 bp
  45% of sequence in fragments shorter than    5786 bp
  50% of sequence in fragments shorter than    6396 bp
  55% of sequence in fragments shorter than    7043 bp
  60% of sequence in fragments shorter than    7752 bp
  65% of sequence in fragments shorter than    8540 bp
  70% of sequence in fragments shorter than    9433 bp
  75% of sequence in fragments shorter than   10493 bp
  80% of sequence in fragments shorter than   11775 bp
  85% of sequence in fragments shorter than   13430 bp
  90% of sequence in fragments shorter than   15923 bp
  95% of sequence in fragments shorter than   21062 bp

# BceAI.ClaI (ACGGC+ATCGAT) double digest, 791 thousand fragments (705178+86477 sites), 3766 bp average fragment length
   5% of sequence in fragments shorter than    1247 bp
  10% of sequence in fragments shorter than    2015 bp
  15% of sequence in fragments shorter than    2725 bp
  20% of sequence in fragments shorter than    3406 bp
  25% of sequence in fragments shorter than    4089 bp
  30% of sequence in fragments shorter than    4791 bp
  35% of sequence in fragments shorter than    5519 bp
  40% of sequence in fragments shorter than    6275 bp
  45% of sequence in fragments shorter than    7084 bp
  50% of sequence in fragments shorter than    7949 bp
  55% of sequence in fragments shorter than    8885 bp
  60% of sequence in fragments shorter than    9908 bp
  65% of sequence in fragments shorter than   11059 bp
  70% of sequence in fragments shorter than   12398 bp
  75% of sequence in fragments shorter than   13971 bp
  80% of sequence in fragments shorter than   15877 bp
  85% of sequence in fragments shorter than   18382 bp
  90% of sequence in fragments shorter than   22017 bp
  95% of sequence in fragments shorter than   28743 bp

# NcoI.SalI (CCATGG+GTCGAC) double digest, 790 thousand fragments (759594+31396 sites), 3769 bp average fragment length
   5% of sequence in fragments shorter than    1306 bp
  10% of sequence in fragments shorter than    1998 bp
  15% of sequence in fragments shorter than    2610 bp
  20% of sequence in fragments shorter than    3192 bp
  25% of sequence in fragments shorter than    3766 bp
  30% of sequence in fragments shorter than    4337 bp
  35% of sequence in fragments shorter than    4894 bp
  40% of sequence in fragments shorter than    5490 bp
  45% of sequence in fragments shorter than    6104 bp
  50% of sequence in fragments shorter than    6778 bp
  55% of sequence in fragments shorter than    7512 bp
  60% of sequence in fragments shorter than    8314 bp
  65% of sequence in fragments shorter than    9217 bp
  70% of sequence in fragments shorter than   10243 bp
  75% of sequence in fragments shorter than   11454 bp
  80% of sequence in fragments shorter than   12910 bp
  85% of sequence in fragments shorter than   14838 bp
  90% of sequence in fragments shorter than   17683 bp
  95% of sequence in fragments shorter than   23348 bp

# MfeI.SwaI (CAATTG+ATTTAAAT) double digest, 790 thousand fragments (564303+226602 sites), 3769 bp average fragment length
   5% of sequence in fragments shorter than    1285 bp
  10% of sequence in fragments shorter than    1969 bp
  15% of sequence in fragments shorter than    2549 bp
  20% of sequence in fragments shorter than    3106 bp
  25% of sequence in fragments shorter than    3662 bp
  30% of sequence in fragments shorter than    4222 bp
  35% of sequence in fragments shorter than    4788 bp
  40% of sequence in fragments shorter than    5380 bp
  45% of sequence in fragments shorter than    6007 bp
  50% of sequence in fragments shorter than    6686 bp
  55% of sequence in fragments shorter than    7427 bp
  60% of sequence in fragments shorter than    8259 bp
  65% of sequence in fragments shorter than    9211 bp
  70% of sequence in fragments shorter than   10324 bp
  75% of sequence in fragments shorter than   11649 bp
  80% of sequence in fragments shorter than   13322 bp
  85% of sequence in fragments shorter than   15673 bp
  90% of sequence in fragments shorter than   19419 bp
  95% of sequence in fragments shorter than   27829 bp

# BglII.SgrAI (AGATCT+CRCCGGYG) double digest, 789 thousand fragments (774732+15270 sites), 3773 bp average fragment length
   5% of sequence in fragments shorter than    1316 bp
  10% of sequence in fragments shorter than    1961 bp
  15% of sequence in fragments shorter than    2545 bp
  20% of sequence in fragments shorter than    3101 bp
  25% of sequence in fragments shorter than    3639 bp
  30% of sequence in fragments shorter than    4175 bp
  35% of sequence in fragments shorter than    4713 bp
  40% of sequence in fragments shorter than    5262 bp
  45% of sequence in fragments shorter than    5844 bp
  50% of sequence in fragments shorter than    6463 bp
  55% of sequence in fragments shorter than    7129 bp
  60% of sequence in fragments shorter than    7861 bp
  65% of sequence in fragments shorter than    8665 bp
  70% of sequence in fragments shorter than    9595 bp
  75% of sequence in fragments shorter than   10677 bp
  80% of sequence in fragments shorter than   11982 bp
  85% of sequence in fragments shorter than   13746 bp
  90% of sequence in fragments shorter than   16239 bp
  95% of sequence in fragments shorter than   21471 bp

# HpaI.SpeI (GTTAAC+ACTAGT) double digest, 789 thousand fragments (389418+400286 sites), 3775 bp average fragment length
   5% of sequence in fragments shorter than    1319 bp
  10% of sequence in fragments shorter than    1986 bp
  15% of sequence in fragments shorter than    2565 bp
  20% of sequence in fragments shorter than    3085 bp
  25% of sequence in fragments shorter than    3626 bp
  30% of sequence in fragments shorter than    4170 bp
  35% of sequence in fragments shorter than    4734 bp
  40% of sequence in fragments shorter than    5320 bp
  45% of sequence in fragments shorter than    5927 bp
  50% of sequence in fragments shorter than    6588 bp
  55% of sequence in fragments shorter than    7290 bp
  60% of sequence in fragments shorter than    8087 bp
  65% of sequence in fragments shorter than    8974 bp
  70% of sequence in fragments shorter than   10004 bp
  75% of sequence in fragments shorter than   11232 bp
  80% of sequence in fragments shorter than   12737 bp
  85% of sequence in fragments shorter than   14811 bp
  90% of sequence in fragments shorter than   18036 bp
  95% of sequence in fragments shorter than   25208 bp

# BglII.NruI (AGATCT+TCGCGA) double digest, 789 thousand fragments (774732+14872 sites), 3775 bp average fragment length
   5% of sequence in fragments shorter than    1317 bp
  10% of sequence in fragments shorter than    1961 bp
  15% of sequence in fragments shorter than    2545 bp
  20% of sequence in fragments shorter than    3101 bp
  25% of sequence in fragments shorter than    3639 bp
  30% of sequence in fragments shorter than    4174 bp
  35% of sequence in fragments shorter than    4711 bp
  40% of sequence in fragments shorter than    5263 bp
  45% of sequence in fragments shorter than    5845 bp
  50% of sequence in fragments shorter than    6464 bp
  55% of sequence in fragments shorter than    7129 bp
  60% of sequence in fragments shorter than    7862 bp
  65% of sequence in fragments shorter than    8668 bp
  70% of sequence in fragments shorter than    9602 bp
  75% of sequence in fragments shorter than   10682 bp
  80% of sequence in fragments shorter than   11999 bp
  85% of sequence in fragments shorter than   13764 bp
  90% of sequence in fragments shorter than   16271 bp
  95% of sequence in fragments shorter than   21500 bp

# HaeII.PacI (RGCGCY+TTAATTAA) double digest, 789 thousand fragments (630101+159455 sites), 3776 bp average fragment length
   5% of sequence in fragments shorter than    1195 bp
  10% of sequence in fragments shorter than    2091 bp
  15% of sequence in fragments shorter than    2936 bp
  20% of sequence in fragments shorter than    3735 bp
  25% of sequence in fragments shorter than    4525 bp
  30% of sequence in fragments shorter than    5329 bp
  35% of sequence in fragments shorter than    6145 bp
  40% of sequence in fragments shorter than    7006 bp
  45% of sequence in fragments shorter than    7918 bp
  50% of sequence in fragments shorter than    8884 bp
  55% of sequence in fragments shorter than    9936 bp
  60% of sequence in fragments shorter than   11096 bp
  65% of sequence in fragments shorter than   12367 bp
  70% of sequence in fragments shorter than   13871 bp
  75% of sequence in fragments shorter than   15600 bp
  80% of sequence in fragments shorter than   17673 bp
  85% of sequence in fragments shorter than   20357 bp
  90% of sequence in fragments shorter than   24013 bp
  95% of sequence in fragments shorter than   30465 bp

# BglII.PvuI (AGATCT+CGATCG) double digest, 788 thousand fragments (774732+14186 sites), 3779 bp average fragment length
   5% of sequence in fragments shorter than    1319 bp
  10% of sequence in fragments shorter than    1958 bp
  15% of sequence in fragments shorter than    2545 bp
  20% of sequence in fragments shorter than    3104 bp
  25% of sequence in fragments shorter than    3643 bp
  30% of sequence in fragments shorter than    4180 bp
  35% of sequence in fragments shorter than    4718 bp
  40% of sequence in fragments shorter than    5271 bp
  45% of sequence in fragments shorter than    5856 bp
  50% of sequence in fragments shorter than    6477 bp
  55% of sequence in fragments shorter than    7144 bp
  60% of sequence in fragments shorter than    7882 bp
  65% of sequence in fragments shorter than    8689 bp
  70% of sequence in fragments shorter than    9625 bp
  75% of sequence in fragments shorter than   10709 bp
  80% of sequence in fragments shorter than   12020 bp
  85% of sequence in fragments shorter than   13796 bp
  90% of sequence in fragments shorter than   16297 bp
  95% of sequence in fragments shorter than   21447 bp

# BglII.FseI (AGATCT+GGCCGGCC) double digest, 788 thousand fragments (774732+13662 sites), 3781 bp average fragment length
   5% of sequence in fragments shorter than    1320 bp
  10% of sequence in fragments shorter than    1965 bp
  15% of sequence in fragments shorter than    2551 bp
  20% of sequence in fragments shorter than    3108 bp
  25% of sequence in fragments shorter than    3646 bp
  30% of sequence in fragments shorter than    4182 bp
  35% of sequence in fragments shorter than    4720 bp
  40% of sequence in fragments shorter than    5272 bp
  45% of sequence in fragments shorter than    5854 bp
  50% of sequence in fragments shorter than    6472 bp
  55% of sequence in fragments shorter than    7139 bp
  60% of sequence in fragments shorter than    7870 bp
  65% of sequence in fragments shorter than    8672 bp
  70% of sequence in fragments shorter than    9603 bp
  75% of sequence in fragments shorter than   10679 bp
  80% of sequence in fragments shorter than   11984 bp
  85% of sequence in fragments shorter than   13740 bp
  90% of sequence in fragments shorter than   16231 bp
  95% of sequence in fragments shorter than   21417 bp

# HaeII.Hpy99I (RGCGCY+CGWCG) double digest, 787 thousand fragments (630101+157917 sites), 3783 bp average fragment length
   5% of sequence in fragments shorter than    1139 bp
  10% of sequence in fragments shorter than    2143 bp
  15% of sequence in fragments shorter than    3139 bp
  20% of sequence in fragments shorter than    4110 bp
  25% of sequence in fragments shorter than    5113 bp
  30% of sequence in fragments shorter than    6143 bp
  35% of sequence in fragments shorter than    7237 bp
  40% of sequence in fragments shorter than    8417 bp
  45% of sequence in fragments shorter than    9677 bp
  50% of sequence in fragments shorter than   11049 bp
  55% of sequence in fragments shorter than   12568 bp
  60% of sequence in fragments shorter than   14273 bp
  65% of sequence in fragments shorter than   16236 bp
  70% of sequence in fragments shorter than   18460 bp
  75% of sequence in fragments shorter than   21125 bp
  80% of sequence in fragments shorter than   24398 bp
  85% of sequence in fragments shorter than   28538 bp
  90% of sequence in fragments shorter than   34532 bp
  95% of sequence in fragments shorter than   44827 bp

# AgeI.FnuDII (ACCGGT+CGCG) double digest, 786 thousand fragments (53386+733940 sites), 3786 bp average fragment length
   5% of sequence in fragments shorter than    1664 bp
  10% of sequence in fragments shorter than    2916 bp
  15% of sequence in fragments shorter than    4113 bp
  20% of sequence in fragments shorter than    5319 bp
  25% of sequence in fragments shorter than    6554 bp
  30% of sequence in fragments shorter than    7871 bp
  35% of sequence in fragments shorter than    9277 bp
  40% of sequence in fragments shorter than   10788 bp
  45% of sequence in fragments shorter than   12427 bp
  50% of sequence in fragments shorter than   14238 bp
  55% of sequence in fragments shorter than   16249 bp
  60% of sequence in fragments shorter than   18517 bp
  65% of sequence in fragments shorter than   21118 bp
  70% of sequence in fragments shorter than   24146 bp
  75% of sequence in fragments shorter than   27690 bp
  80% of sequence in fragments shorter than   32115 bp
  85% of sequence in fragments shorter than   38173 bp
  90% of sequence in fragments shorter than   46734 bp
  95% of sequence in fragments shorter than   63757 bp

# BsrBI.SphI (CCGCTC+GCATGC) double digest, 786 thousand fragments (235523+550952 sites), 3791 bp average fragment length
   5% of sequence in fragments shorter than    1301 bp
  10% of sequence in fragments shorter than    2007 bp
  15% of sequence in fragments shorter than    2639 bp
  20% of sequence in fragments shorter than    3238 bp
  25% of sequence in fragments shorter than    3835 bp
  30% of sequence in fragments shorter than    4440 bp
  35% of sequence in fragments shorter than    5066 bp
  40% of sequence in fragments shorter than    5716 bp
  45% of sequence in fragments shorter than    6406 bp
  50% of sequence in fragments shorter than    7136 bp
  55% of sequence in fragments shorter than    7927 bp
  60% of sequence in fragments shorter than    8799 bp
  65% of sequence in fragments shorter than    9788 bp
  70% of sequence in fragments shorter than   10931 bp
  75% of sequence in fragments shorter than   12289 bp
  80% of sequence in fragments shorter than   13940 bp
  85% of sequence in fragments shorter than   16100 bp
  90% of sequence in fragments shorter than   19160 bp
  95% of sequence in fragments shorter than   24976 bp

# AatII.BsaAI (GACGTC+YACGTR) double digest, 784 thousand fragments (71089+713801 sites), 3798 bp average fragment length
   5% of sequence in fragments shorter than    1341 bp
  10% of sequence in fragments shorter than    2030 bp
  15% of sequence in fragments shorter than    2653 bp
  20% of sequence in fragments shorter than    3233 bp
  25% of sequence in fragments shorter than    3782 bp
  30% of sequence in fragments shorter than    4357 bp
  35% of sequence in fragments shorter than    4932 bp
  40% of sequence in fragments shorter than    5537 bp
  45% of sequence in fragments shorter than    6175 bp
  50% of sequence in fragments shorter than    6849 bp
  55% of sequence in fragments shorter than    7573 bp
  60% of sequence in fragments shorter than    8373 bp
  65% of sequence in fragments shorter than    9253 bp
  70% of sequence in fragments shorter than   10261 bp
  75% of sequence in fragments shorter than   11435 bp
  80% of sequence in fragments shorter than   12844 bp
  85% of sequence in fragments shorter than   14668 bp
  90% of sequence in fragments shorter than   17272 bp
  95% of sequence in fragments shorter than   22078 bp

# BglII.NotI (AGATCT+GCGGCCGC) double digest, 784 thousand fragments (774732+9795 sites), 3800 bp average fragment length
   5% of sequence in fragments shorter than    1325 bp
  10% of sequence in fragments shorter than    1974 bp
  15% of sequence in fragments shorter than    2565 bp
  20% of sequence in fragments shorter than    3125 bp
  25% of sequence in fragments shorter than    3666 bp
  30% of sequence in fragments shorter than    4206 bp
  35% of sequence in fragments shorter than    4747 bp
  40% of sequence in fragments shorter than    5303 bp
  45% of sequence in fragments shorter than    5892 bp
  50% of sequence in fragments shorter than    6513 bp
  55% of sequence in fragments shorter than    7186 bp
  60% of sequence in fragments shorter than    7925 bp
  65% of sequence in fragments shorter than    8734 bp
  70% of sequence in fragments shorter than    9673 bp
  75% of sequence in fragments shorter than   10760 bp
  80% of sequence in fragments shorter than   12082 bp
  85% of sequence in fragments shorter than   13860 bp
  90% of sequence in fragments shorter than   16402 bp
  95% of sequence in fragments shorter than   21721 bp

# BglII.RsrII (AGATCT+CGGWCCG) double digest, 784 thousand fragments (774732+9764 sites), 3800 bp average fragment length
   5% of sequence in fragments shorter than    1325 bp
  10% of sequence in fragments shorter than    1973 bp
  15% of sequence in fragments shorter than    2562 bp
  20% of sequence in fragments shorter than    3123 bp
  25% of sequence in fragments shorter than    3664 bp
  30% of sequence in fragments shorter than    4204 bp
  35% of sequence in fragments shorter than    4746 bp
  40% of sequence in fragments shorter than    5301 bp
  45% of sequence in fragments shorter than    5890 bp
  50% of sequence in fragments shorter than    6513 bp
  55% of sequence in fragments shorter than    7186 bp
  60% of sequence in fragments shorter than    7926 bp
  65% of sequence in fragments shorter than    8737 bp
  70% of sequence in fragments shorter than    9677 bp
  75% of sequence in fragments shorter than   10757 bp
  80% of sequence in fragments shorter than   12091 bp
  85% of sequence in fragments shorter than   13872 bp
  90% of sequence in fragments shorter than   16419 bp
  95% of sequence in fragments shorter than   21720 bp

# ApaLI.KpnI (GTGCAC+GGTACC) double digest, 783 thousand fragments (496255+287974 sites), 3801 bp average fragment length
   5% of sequence in fragments shorter than    1310 bp
  10% of sequence in fragments shorter than    1929 bp
  15% of sequence in fragments shorter than    2550 bp
  20% of sequence in fragments shorter than    3118 bp
  25% of sequence in fragments shorter than    3678 bp
  30% of sequence in fragments shorter than    4250 bp
  35% of sequence in fragments shorter than    4827 bp
  40% of sequence in fragments shorter than    5428 bp
  45% of sequence in fragments shorter than    6063 bp
  50% of sequence in fragments shorter than    6735 bp
  55% of sequence in fragments shorter than    7454 bp
  60% of sequence in fragments shorter than    8241 bp
  65% of sequence in fragments shorter than    9129 bp
  70% of sequence in fragments shorter than   10123 bp
  75% of sequence in fragments shorter than   11296 bp
  80% of sequence in fragments shorter than   12731 bp
  85% of sequence in fragments shorter than   14578 bp
  90% of sequence in fragments shorter than   17281 bp
  95% of sequence in fragments shorter than   22811 bp

# ApaLI.NheI (GTGCAC+GCTAGC) double digest, 783 thousand fragments (496255+287384 sites), 3804 bp average fragment length
   5% of sequence in fragments shorter than    1319 bp
  10% of sequence in fragments shorter than    1969 bp
  15% of sequence in fragments shorter than    2584 bp
  20% of sequence in fragments shorter than    3110 bp
  25% of sequence in fragments shorter than    3680 bp
  30% of sequence in fragments shorter than    4248 bp
  35% of sequence in fragments shorter than    4825 bp
  40% of sequence in fragments shorter than    5427 bp
  45% of sequence in fragments shorter than    6052 bp
  50% of sequence in fragments shorter than    6723 bp
  55% of sequence in fragments shorter than    7434 bp
  60% of sequence in fragments shorter than    8213 bp
  65% of sequence in fragments shorter than    9077 bp
  70% of sequence in fragments shorter than   10061 bp
  75% of sequence in fragments shorter than   11209 bp
  80% of sequence in fragments shorter than   12616 bp
  85% of sequence in fragments shorter than   14451 bp
  90% of sequence in fragments shorter than   17057 bp
  95% of sequence in fragments shorter than   22054 bp

# BciVI.ClaI (GTATCC+ATCGAT) double digest, 782 thousand fragments (696093+86477 sites), 3810 bp average fragment length
   5% of sequence in fragments shorter than    1349 bp
  10% of sequence in fragments shorter than    2012 bp
  15% of sequence in fragments shorter than    2594 bp
  20% of sequence in fragments shorter than    3153 bp
  25% of sequence in fragments shorter than    3702 bp
  30% of sequence in fragments shorter than    4238 bp
  35% of sequence in fragments shorter than    4792 bp
  40% of sequence in fragments shorter than    5359 bp
  45% of sequence in fragments shorter than    5954 bp
  50% of sequence in fragments shorter than    6584 bp
  55% of sequence in fragments shorter than    7258 bp
  60% of sequence in fragments shorter than    8000 bp
  65% of sequence in fragments shorter than    8821 bp
  70% of sequence in fragments shorter than    9748 bp
  75% of sequence in fragments shorter than   10852 bp
  80% of sequence in fragments shorter than   12162 bp
  85% of sequence in fragments shorter than   13892 bp
  90% of sequence in fragments shorter than   16463 bp
  95% of sequence in fragments shorter than   21219 bp

# BstEII.EcoRV (GGTNACC+GATATC) double digest, 781 thousand fragments (335865+446473 sites), 3811 bp average fragment length
   5% of sequence in fragments shorter than    1327 bp
  10% of sequence in fragments shorter than    2005 bp
  15% of sequence in fragments shorter than    2616 bp
  20% of sequence in fragments shorter than    3189 bp
  25% of sequence in fragments shorter than    3741 bp
  30% of sequence in fragments shorter than    4293 bp
  35% of sequence in fragments shorter than    4849 bp
  40% of sequence in fragments shorter than    5421 bp
  45% of sequence in fragments shorter than    6023 bp
  50% of sequence in fragments shorter than    6657 bp
  55% of sequence in fragments shorter than    7340 bp
  60% of sequence in fragments shorter than    8071 bp
  65% of sequence in fragments shorter than    8890 bp
  70% of sequence in fragments shorter than    9818 bp
  75% of sequence in fragments shorter than   10922 bp
  80% of sequence in fragments shorter than   12244 bp
  85% of sequence in fragments shorter than   13943 bp
  90% of sequence in fragments shorter than   16377 bp
  95% of sequence in fragments shorter than   20913 bp

# KasI.SphI (GGCGCC+GCATGC) double digest, 780 thousand fragments (230381+550952 sites), 3815 bp average fragment length
   5% of sequence in fragments shorter than    1298 bp
  10% of sequence in fragments shorter than    1996 bp
  15% of sequence in fragments shorter than    2622 bp
  20% of sequence in fragments shorter than    3218 bp
  25% of sequence in fragments shorter than    3812 bp
  30% of sequence in fragments shorter than    4413 bp
  35% of sequence in fragments shorter than    5039 bp
  40% of sequence in fragments shorter than    5692 bp
  45% of sequence in fragments shorter than    6376 bp
  50% of sequence in fragments shorter than    7103 bp
  55% of sequence in fragments shorter than    7892 bp
  60% of sequence in fragments shorter than    8767 bp
  65% of sequence in fragments shorter than    9761 bp
  70% of sequence in fragments shorter than   10917 bp
  75% of sequence in fragments shorter than   12279 bp
  80% of sequence in fragments shorter than   13937 bp
  85% of sequence in fragments shorter than   16144 bp
  90% of sequence in fragments shorter than   19304 bp
  95% of sequence in fragments shorter than   25742 bp

# MluI.NcoI (ACGCGT+CCATGG) double digest, 780 thousand fragments (21415+759594 sites), 3817 bp average fragment length
   5% of sequence in fragments shorter than    1323 bp
  10% of sequence in fragments shorter than    2024 bp
  15% of sequence in fragments shorter than    2648 bp
  20% of sequence in fragments shorter than    3240 bp
  25% of sequence in fragments shorter than    3821 bp
  30% of sequence in fragments shorter than    4401 bp
  35% of sequence in fragments shorter than    4968 bp
  40% of sequence in fragments shorter than    5571 bp
  45% of sequence in fragments shorter than    6193 bp
  50% of sequence in fragments shorter than    6876 bp
  55% of sequence in fragments shorter than    7619 bp
  60% of sequence in fragments shorter than    8442 bp
  65% of sequence in fragments shorter than    9352 bp
  70% of sequence in fragments shorter than   10401 bp
  75% of sequence in fragments shorter than   11640 bp
  80% of sequence in fragments shorter than   13132 bp
  85% of sequence in fragments shorter than   15099 bp
  90% of sequence in fragments shorter than   18004 bp
  95% of sequence in fragments shorter than   23787 bp

# BclI.PmeI (TGATCA+GTTTAAAC) double digest, 780 thousand fragments (738785+42094 sites), 3818 bp average fragment length
   5% of sequence in fragments shorter than    1345 bp
  10% of sequence in fragments shorter than    2014 bp
  15% of sequence in fragments shorter than    2598 bp
  20% of sequence in fragments shorter than    3137 bp
  25% of sequence in fragments shorter than    3678 bp
  30% of sequence in fragments shorter than    4207 bp
  35% of sequence in fragments shorter than    4746 bp
  40% of sequence in fragments shorter than    5296 bp
  45% of sequence in fragments shorter than    5878 bp
  50% of sequence in fragments shorter than    6498 bp
  55% of sequence in fragments shorter than    7163 bp
  60% of sequence in fragments shorter than    7890 bp
  65% of sequence in fragments shorter than    8700 bp
  70% of sequence in fragments shorter than    9632 bp
  75% of sequence in fragments shorter than   10736 bp
  80% of sequence in fragments shorter than   12077 bp
  85% of sequence in fragments shorter than   13810 bp
  90% of sequence in fragments shorter than   16461 bp
  95% of sequence in fragments shorter than   22127 bp

# BsaAI.SacII (YACGTR+CCGCGG) double digest, 780 thousand fragments (713801+66901 sites), 3819 bp average fragment length
   5% of sequence in fragments shorter than    1354 bp
  10% of sequence in fragments shorter than    2082 bp
  15% of sequence in fragments shorter than    2746 bp
  20% of sequence in fragments shorter than    3351 bp
  25% of sequence in fragments shorter than    3928 bp
  30% of sequence in fragments shorter than    4522 bp
  35% of sequence in fragments shorter than    5140 bp
  40% of sequence in fragments shorter than    5777 bp
  45% of sequence in fragments shorter than    6445 bp
  50% of sequence in fragments shorter than    7151 bp
  55% of sequence in fragments shorter than    7922 bp
  60% of sequence in fragments shorter than    8755 bp
  65% of sequence in fragments shorter than    9691 bp
  70% of sequence in fragments shorter than   10753 bp
  75% of sequence in fragments shorter than   11979 bp
  80% of sequence in fragments shorter than   13468 bp
  85% of sequence in fragments shorter than   15407 bp
  90% of sequence in fragments shorter than   18144 bp
  95% of sequence in fragments shorter than   23096 bp

# AscI.BglII (GGCGCGCC+AGATCT) double digest, 779 thousand fragments (4751+774732 sites), 3824 bp average fragment length
   5% of sequence in fragments shorter than    1333 bp
  10% of sequence in fragments shorter than    1984 bp
  15% of sequence in fragments shorter than    2578 bp
  20% of sequence in fragments shorter than    3144 bp
  25% of sequence in fragments shorter than    3686 bp
  30% of sequence in fragments shorter than    4231 bp
  35% of sequence in fragments shorter than    4775 bp
  40% of sequence in fragments shorter than    5334 bp
  45% of sequence in fragments shorter than    5927 bp
  50% of sequence in fragments shorter than    6559 bp
  55% of sequence in fragments shorter than    7236 bp
  60% of sequence in fragments shorter than    7979 bp
  65% of sequence in fragments shorter than    8800 bp
  70% of sequence in fragments shorter than    9753 bp
  75% of sequence in fragments shorter than   10847 bp
  80% of sequence in fragments shorter than   12198 bp
  85% of sequence in fragments shorter than   13988 bp
  90% of sequence in fragments shorter than   16579 bp
  95% of sequence in fragments shorter than   22005 bp

# BsrBI.ScaI (CCGCTC+AGTACT) double digest, 778 thousand fragments (235523+543793 sites), 3825 bp average fragment length
   5% of sequence in fragments shorter than    1350 bp
  10% of sequence in fragments shorter than    2046 bp
  15% of sequence in fragments shorter than    2665 bp
  20% of sequence in fragments shorter than    3241 bp
  25% of sequence in fragments shorter than    3805 bp
  30% of sequence in fragments shorter than    4368 bp
  35% of sequence in fragments shorter than    4944 bp
  40% of sequence in fragments shorter than    5534 bp
  45% of sequence in fragments shorter than    6156 bp
  50% of sequence in fragments shorter than    6814 bp
  55% of sequence in fragments shorter than    7517 bp
  60% of sequence in fragments shorter than    8271 bp
  65% of sequence in fragments shorter than    9117 bp
  70% of sequence in fragments shorter than   10079 bp
  75% of sequence in fragments shorter than   11215 bp
  80% of sequence in fragments shorter than   12583 bp
  85% of sequence in fragments shorter than   14348 bp
  90% of sequence in fragments shorter than   16841 bp
  95% of sequence in fragments shorter than   21695 bp

# SphI.SwaI (GCATGC+ATTTAAAT) double digest, 777 thousand fragments (550952+226602 sites), 3834 bp average fragment length
   5% of sequence in fragments shorter than    1350 bp
  10% of sequence in fragments shorter than    2033 bp
  15% of sequence in fragments shorter than    2631 bp
  20% of sequence in fragments shorter than    3189 bp
  25% of sequence in fragments shorter than    3738 bp
  30% of sequence in fragments shorter than    4285 bp
  35% of sequence in fragments shorter than    4849 bp
  40% of sequence in fragments shorter than    5424 bp
  45% of sequence in fragments shorter than    6028 bp
  50% of sequence in fragments shorter than    6662 bp
  55% of sequence in fragments shorter than    7344 bp
  60% of sequence in fragments shorter than    8091 bp
  65% of sequence in fragments shorter than    8909 bp
  70% of sequence in fragments shorter than    9850 bp
  75% of sequence in fragments shorter than   10963 bp
  80% of sequence in fragments shorter than   12321 bp
  85% of sequence in fragments shorter than   14070 bp
  90% of sequence in fragments shorter than   16592 bp
  95% of sequence in fragments shorter than   21569 bp

# SapI.SpeI (GCTCTTC+ACTAGT) double digest, 777 thousand fragments (377161+400286 sites), 3834 bp average fragment length
   5% of sequence in fragments shorter than    1349 bp
  10% of sequence in fragments shorter than    2029 bp
  15% of sequence in fragments shorter than    2607 bp
  20% of sequence in fragments shorter than    3155 bp
  25% of sequence in fragments shorter than    3659 bp
  30% of sequence in fragments shorter than    4183 bp
  35% of sequence in fragments shorter than    4736 bp
  40% of sequence in fragments shorter than    5296 bp
  45% of sequence in fragments shorter than    5885 bp
  50% of sequence in fragments shorter than    6509 bp
  55% of sequence in fragments shorter than    7184 bp
  60% of sequence in fragments shorter than    7924 bp
  65% of sequence in fragments shorter than    8743 bp
  70% of sequence in fragments shorter than    9674 bp
  75% of sequence in fragments shorter than   10772 bp
  80% of sequence in fragments shorter than   12085 bp
  85% of sequence in fragments shorter than   13800 bp
  90% of sequence in fragments shorter than   16333 bp
  95% of sequence in fragments shorter than   21360 bp

# SmaI.SpeI (CCCGGG+ACTAGT) double digest, 776 thousand fragments (376940+400286 sites), 3836 bp average fragment length
   5% of sequence in fragments shorter than    1322 bp
  10% of sequence in fragments shorter than    2028 bp
  15% of sequence in fragments shorter than    2640 bp
  20% of sequence in fragments shorter than    3231 bp
  25% of sequence in fragments shorter than    3811 bp
  30% of sequence in fragments shorter than    4394 bp
  35% of sequence in fragments shorter than    4994 bp
  40% of sequence in fragments shorter than    5602 bp
  45% of sequence in fragments shorter than    6254 bp
  50% of sequence in fragments shorter than    6936 bp
  55% of sequence in fragments shorter than    7671 bp
  60% of sequence in fragments shorter than    8472 bp
  65% of sequence in fragments shorter than    9371 bp
  70% of sequence in fragments shorter than   10419 bp
  75% of sequence in fragments shorter than   11640 bp
  80% of sequence in fragments shorter than   13104 bp
  85% of sequence in fragments shorter than   15055 bp
  90% of sequence in fragments shorter than   17873 bp
  95% of sequence in fragments shorter than   23477 bp

# AatII.BceAI (GACGTC+ACGGC) double digest, 775 thousand fragments (71089+705178 sites), 3841 bp average fragment length
   5% of sequence in fragments shorter than    1252 bp
  10% of sequence in fragments shorter than    2063 bp
  15% of sequence in fragments shorter than    2811 bp
  20% of sequence in fragments shorter than    3546 bp
  25% of sequence in fragments shorter than    4283 bp
  30% of sequence in fragments shorter than    5044 bp
  35% of sequence in fragments shorter than    5834 bp
  40% of sequence in fragments shorter than    6656 bp
  45% of sequence in fragments shorter than    7548 bp
  50% of sequence in fragments shorter than    8510 bp
  55% of sequence in fragments shorter than    9548 bp
  60% of sequence in fragments shorter than   10708 bp
  65% of sequence in fragments shorter than   12002 bp
  70% of sequence in fragments shorter than   13486 bp
  75% of sequence in fragments shorter than   15258 bp
  80% of sequence in fragments shorter than   17409 bp
  85% of sequence in fragments shorter than   20202 bp
  90% of sequence in fragments shorter than   24247 bp
  95% of sequence in fragments shorter than   31678 bp

# FnuDII.PmeI (CGCG+GTTTAAAC) double digest, 775 thousand fragments (733940+42094 sites), 3841 bp average fragment length
   5% of sequence in fragments shorter than    1709 bp
  10% of sequence in fragments shorter than    2986 bp
  15% of sequence in fragments shorter than    4185 bp
  20% of sequence in fragments shorter than    5388 bp
  25% of sequence in fragments shorter than    6625 bp
  30% of sequence in fragments shorter than    7929 bp
  35% of sequence in fragments shorter than    9309 bp
  40% of sequence in fragments shorter than   10776 bp
  45% of sequence in fragments shorter than   12366 bp
  50% of sequence in fragments shorter than   14132 bp
  55% of sequence in fragments shorter than   16093 bp
  60% of sequence in fragments shorter than   18250 bp
  65% of sequence in fragments shorter than   20759 bp
  70% of sequence in fragments shorter than   23642 bp
  75% of sequence in fragments shorter than   27046 bp
  80% of sequence in fragments shorter than   31279 bp
  85% of sequence in fragments shorter than   36983 bp
  90% of sequence in fragments shorter than   45725 bp
  95% of sequence in fragments shorter than   61565 bp

# AclI.SacI (AACGTT+GAGCTC) double digest, 775 thousand fragments (172706+603323 sites), 3842 bp average fragment length
   5% of sequence in fragments shorter than    1318 bp
  10% of sequence in fragments shorter than    2017 bp
  15% of sequence in fragments shorter than    2612 bp
  20% of sequence in fragments shorter than    3172 bp
  25% of sequence in fragments shorter than    3746 bp
  30% of sequence in fragments shorter than    4319 bp
  35% of sequence in fragments shorter than    4897 bp
  40% of sequence in fragments shorter than    5500 bp
  45% of sequence in fragments shorter than    6138 bp
  50% of sequence in fragments shorter than    6815 bp
  55% of sequence in fragments shorter than    7543 bp
  60% of sequence in fragments shorter than    8319 bp
  65% of sequence in fragments shorter than    9200 bp
  70% of sequence in fragments shorter than   10205 bp
  75% of sequence in fragments shorter than   11383 bp
  80% of sequence in fragments shorter than   12803 bp
  85% of sequence in fragments shorter than   14625 bp
  90% of sequence in fragments shorter than   17153 bp
  95% of sequence in fragments shorter than   22137 bp

# AflII.SnaBI (CTTAAG+TACGTA) double digest, 775 thousand fragments (643284+132299 sites), 3844 bp average fragment length
   5% of sequence in fragments shorter than    1358 bp
  10% of sequence in fragments shorter than    2030 bp
  15% of sequence in fragments shorter than    2622 bp
  20% of sequence in fragments shorter than    3172 bp
  25% of sequence in fragments shorter than    3711 bp
  30% of sequence in fragments shorter than    4256 bp
  35% of sequence in fragments shorter than    4809 bp
  40% of sequence in fragments shorter than    5375 bp
  45% of sequence in fragments shorter than    5974 bp
  50% of sequence in fragments shorter than    6611 bp
  55% of sequence in fragments shorter than    7305 bp
  60% of sequence in fragments shorter than    8068 bp
  65% of sequence in fragments shorter than    8925 bp
  70% of sequence in fragments shorter than    9901 bp
  75% of sequence in fragments shorter than   11059 bp
  80% of sequence in fragments shorter than   12489 bp
  85% of sequence in fragments shorter than   14399 bp
  90% of sequence in fragments shorter than   17277 bp
  95% of sequence in fragments shorter than   23546 bp

# NcoI.SgrAI (CCATGG+CRCCGGYG) double digest, 774 thousand fragments (759594+15270 sites), 3847 bp average fragment length
   5% of sequence in fragments shorter than    1333 bp
  10% of sequence in fragments shorter than    2041 bp
  15% of sequence in fragments shorter than    2672 bp
  20% of sequence in fragments shorter than    3269 bp
  25% of sequence in fragments shorter than    3855 bp
  30% of sequence in fragments shorter than    4438 bp
  35% of sequence in fragments shorter than    5013 bp
  40% of sequence in fragments shorter than    5621 bp
  45% of sequence in fragments shorter than    6249 bp
  50% of sequence in fragments shorter than    6942 bp
  55% of sequence in fragments shorter than    7693 bp
  60% of sequence in fragments shorter than    8522 bp
  65% of sequence in fragments shorter than    9441 bp
  70% of sequence in fragments shorter than   10498 bp
  75% of sequence in fragments shorter than   11742 bp
  80% of sequence in fragments shorter than   13250 bp
  85% of sequence in fragments shorter than   15242 bp
  90% of sequence in fragments shorter than   18191 bp
  95% of sequence in fragments shorter than   24030 bp

# NcoI.NruI (CCATGG+TCGCGA) double digest, 774 thousand fragments (759594+14872 sites), 3849 bp average fragment length
   5% of sequence in fragments shorter than    1335 bp
  10% of sequence in fragments shorter than    2041 bp
  15% of sequence in fragments shorter than    2668 bp
  20% of sequence in fragments shorter than    3262 bp
  25% of sequence in fragments shorter than    3846 bp
  30% of sequence in fragments shorter than    4427 bp
  35% of sequence in fragments shorter than    4998 bp
  40% of sequence in fragments shorter than    5600 bp
  45% of sequence in fragments shorter than    6226 bp
  50% of sequence in fragments shorter than    6915 bp
  55% of sequence in fragments shorter than    7659 bp
  60% of sequence in fragments shorter than    8483 bp
  65% of sequence in fragments shorter than    9396 bp
  70% of sequence in fragments shorter than   10449 bp
  75% of sequence in fragments shorter than   11685 bp
  80% of sequence in fragments shorter than   13186 bp
  85% of sequence in fragments shorter than   15136 bp
  90% of sequence in fragments shorter than   18064 bp
  95% of sequence in fragments shorter than   23798 bp

# KasI.ScaI (GGCGCC+AGTACT) double digest, 773 thousand fragments (230381+543793 sites), 3851 bp average fragment length
   5% of sequence in fragments shorter than    1344 bp
  10% of sequence in fragments shorter than    2038 bp
  15% of sequence in fragments shorter than    2649 bp
  20% of sequence in fragments shorter than    3228 bp
  25% of sequence in fragments shorter than    3797 bp
  30% of sequence in fragments shorter than    4361 bp
  35% of sequence in fragments shorter than    4944 bp
  40% of sequence in fragments shorter than    5544 bp
  45% of sequence in fragments shorter than    6175 bp
  50% of sequence in fragments shorter than    6841 bp
  55% of sequence in fragments shorter than    7559 bp
  60% of sequence in fragments shorter than    8337 bp
  65% of sequence in fragments shorter than    9206 bp
  70% of sequence in fragments shorter than   10184 bp
  75% of sequence in fragments shorter than   11363 bp
  80% of sequence in fragments shorter than   12815 bp
  85% of sequence in fragments shorter than   14738 bp
  90% of sequence in fragments shorter than   17481 bp
  95% of sequence in fragments shorter than   23085 bp

# NcoI.PvuI (CCATGG+CGATCG) double digest, 773 thousand fragments (759594+14186 sites), 3853 bp average fragment length
   5% of sequence in fragments shorter than    1338 bp
  10% of sequence in fragments shorter than    2036 bp
  15% of sequence in fragments shorter than    2667 bp
  20% of sequence in fragments shorter than    3262 bp
  25% of sequence in fragments shorter than    3848 bp
  30% of sequence in fragments shorter than    4430 bp
  35% of sequence in fragments shorter than    5001 bp
  40% of sequence in fragments shorter than    5608 bp
  45% of sequence in fragments shorter than    6235 bp
  50% of sequence in fragments shorter than    6926 bp
  55% of sequence in fragments shorter than    7674 bp
  60% of sequence in fragments shorter than    8497 bp
  65% of sequence in fragments shorter than    9408 bp
  70% of sequence in fragments shorter than   10461 bp
  75% of sequence in fragments shorter than   11698 bp
  80% of sequence in fragments shorter than   13200 bp
  85% of sequence in fragments shorter than   15161 bp
  90% of sequence in fragments shorter than   18069 bp
  95% of sequence in fragments shorter than   23793 bp

# FseI.NcoI (GGCCGGCC+CCATGG) double digest, 772 thousand fragments (13662+759594 sites), 3855 bp average fragment length
   5% of sequence in fragments shorter than    1336 bp
  10% of sequence in fragments shorter than    2044 bp
  15% of sequence in fragments shorter than    2677 bp
  20% of sequence in fragments shorter than    3275 bp
  25% of sequence in fragments shorter than    3864 bp
  30% of sequence in fragments shorter than    4448 bp
  35% of sequence in fragments shorter than    5026 bp
  40% of sequence in fragments shorter than    5633 bp
  45% of sequence in fragments shorter than    6265 bp
  50% of sequence in fragments shorter than    6959 bp
  55% of sequence in fragments shorter than    7712 bp
  60% of sequence in fragments shorter than    8544 bp
  65% of sequence in fragments shorter than    9465 bp
  70% of sequence in fragments shorter than   10524 bp
  75% of sequence in fragments shorter than   11779 bp
  80% of sequence in fragments shorter than   13295 bp
  85% of sequence in fragments shorter than   15293 bp
  90% of sequence in fragments shorter than   18256 bp
  95% of sequence in fragments shorter than   24139 bp

# BsaAI.PspXI (YACGTR+VCTCGAGB) double digest, 772 thousand fragments (713801+59193 sites), 3857 bp average fragment length
   5% of sequence in fragments shorter than    1360 bp
  10% of sequence in fragments shorter than    2062 bp
  15% of sequence in fragments shorter than    2695 bp
  20% of sequence in fragments shorter than    3280 bp
  25% of sequence in fragments shorter than    3833 bp
  30% of sequence in fragments shorter than    4416 bp
  35% of sequence in fragments shorter than    4998 bp
  40% of sequence in fragments shorter than    5612 bp
  45% of sequence in fragments shorter than    6257 bp
  50% of sequence in fragments shorter than    6940 bp
  55% of sequence in fragments shorter than    7671 bp
  60% of sequence in fragments shorter than    8475 bp
  65% of sequence in fragments shorter than    9367 bp
  70% of sequence in fragments shorter than   10381 bp
  75% of sequence in fragments shorter than   11561 bp
  80% of sequence in fragments shorter than   12987 bp
  85% of sequence in fragments shorter than   14822 bp
  90% of sequence in fragments shorter than   17452 bp
  95% of sequence in fragments shorter than   22159 bp

# BceAI.SacII (ACGGC+CCGCGG) double digest, 771 thousand fragments (705178+66901 sites), 3861 bp average fragment length
   5% of sequence in fragments shorter than    1287 bp
  10% of sequence in fragments shorter than    2167 bp
  15% of sequence in fragments shorter than    2977 bp
  20% of sequence in fragments shorter than    3775 bp
  25% of sequence in fragments shorter than    4578 bp
  30% of sequence in fragments shorter than    5401 bp
  35% of sequence in fragments shorter than    6250 bp
  40% of sequence in fragments shorter than    7157 bp
  45% of sequence in fragments shorter than    8124 bp
  50% of sequence in fragments shorter than    9158 bp
  55% of sequence in fragments shorter than   10295 bp
  60% of sequence in fragments shorter than   11561 bp
  65% of sequence in fragments shorter than   12967 bp
  70% of sequence in fragments shorter than   14616 bp
  75% of sequence in fragments shorter than   16530 bp
  80% of sequence in fragments shorter than   18893 bp
  85% of sequence in fragments shorter than   22008 bp
  90% of sequence in fragments shorter than   26388 bp
  95% of sequence in fragments shorter than   34843 bp

# ScaI.SwaI (AGTACT+ATTTAAAT) double digest, 770 thousand fragments (543793+226602 sites), 3869 bp average fragment length
   5% of sequence in fragments shorter than    1326 bp
  10% of sequence in fragments shorter than    2010 bp
  15% of sequence in fragments shorter than    2617 bp
  20% of sequence in fragments shorter than    3199 bp
  25% of sequence in fragments shorter than    3771 bp
  30% of sequence in fragments shorter than    4350 bp
  35% of sequence in fragments shorter than    4949 bp
  40% of sequence in fragments shorter than    5581 bp
  45% of sequence in fragments shorter than    6252 bp
  50% of sequence in fragments shorter than    6976 bp
  55% of sequence in fragments shorter than    7767 bp
  60% of sequence in fragments shorter than    8623 bp
  65% of sequence in fragments shorter than    9594 bp
  70% of sequence in fragments shorter than   10732 bp
  75% of sequence in fragments shorter than   12082 bp
  80% of sequence in fragments shorter than   13810 bp
  85% of sequence in fragments shorter than   16164 bp
  90% of sequence in fragments shorter than   19831 bp
  95% of sequence in fragments shorter than   28516 bp

# BclI.SalI (TGATCA+GTCGAC) double digest, 769 thousand fragments (738785+31396 sites), 3871 bp average fragment length
   5% of sequence in fragments shorter than    1367 bp
  10% of sequence in fragments shorter than    2047 bp
  15% of sequence in fragments shorter than    2636 bp
  20% of sequence in fragments shorter than    3182 bp
  25% of sequence in fragments shorter than    3728 bp
  30% of sequence in fragments shorter than    4260 bp
  35% of sequence in fragments shorter than    4807 bp
  40% of sequence in fragments shorter than    5365 bp
  45% of sequence in fragments shorter than    5952 bp
  50% of sequence in fragments shorter than    6579 bp
  55% of sequence in fragments shorter than    7245 bp
  60% of sequence in fragments shorter than    7974 bp
  65% of sequence in fragments shorter than    8791 bp
  70% of sequence in fragments shorter than    9720 bp
  75% of sequence in fragments shorter than   10825 bp
  80% of sequence in fragments shorter than   12154 bp
  85% of sequence in fragments shorter than   13891 bp
  90% of sequence in fragments shorter than   16495 bp
  95% of sequence in fragments shorter than   21891 bp

# NcoI.NotI (CCATGG+GCGGCCGC) double digest, 769 thousand fragments (759594+9795 sites), 3875 bp average fragment length
   5% of sequence in fragments shorter than    1344 bp
  10% of sequence in fragments shorter than    2057 bp
  15% of sequence in fragments shorter than    2690 bp
  20% of sequence in fragments shorter than    3291 bp
  25% of sequence in fragments shorter than    3881 bp
  30% of sequence in fragments shorter than    4467 bp
  35% of sequence in fragments shorter than    5046 bp
  40% of sequence in fragments shorter than    5653 bp
  45% of sequence in fragments shorter than    6287 bp
  50% of sequence in fragments shorter than    6984 bp
  55% of sequence in fragments shorter than    7739 bp
  60% of sequence in fragments shorter than    8572 bp
  65% of sequence in fragments shorter than    9491 bp
  70% of sequence in fragments shorter than   10550 bp
  75% of sequence in fragments shorter than   11811 bp
  80% of sequence in fragments shorter than   13326 bp
  85% of sequence in fragments shorter than   15320 bp
  90% of sequence in fragments shorter than   18287 bp
  95% of sequence in fragments shorter than   24232 bp

# NcoI.RsrII (CCATGG+CGGWCCG) double digest, 768 thousand fragments (759594+9764 sites), 3875 bp average fragment length
   5% of sequence in fragments shorter than    1344 bp
  10% of sequence in fragments shorter than    2055 bp
  15% of sequence in fragments shorter than    2688 bp
  20% of sequence in fragments shorter than    3287 bp
  25% of sequence in fragments shorter than    3876 bp
  30% of sequence in fragments shorter than    4461 bp
  35% of sequence in fragments shorter than    5041 bp
  40% of sequence in fragments shorter than    5646 bp
  45% of sequence in fragments shorter than    6278 bp
  50% of sequence in fragments shorter than    6973 bp
  55% of sequence in fragments shorter than    7728 bp
  60% of sequence in fragments shorter than    8558 bp
  65% of sequence in fragments shorter than    9478 bp
  70% of sequence in fragments shorter than   10540 bp
  75% of sequence in fragments shorter than   11802 bp
  80% of sequence in fragments shorter than   13317 bp
  85% of sequence in fragments shorter than   15300 bp
  90% of sequence in fragments shorter than   18265 bp
  95% of sequence in fragments shorter than   24164 bp

# AflII.NaeI (CTTAAG+GCCGGC) double digest, 768 thousand fragments (643284+125692 sites), 3876 bp average fragment length
   5% of sequence in fragments shorter than    1370 bp
  10% of sequence in fragments shorter than    2089 bp
  15% of sequence in fragments shorter than    2697 bp
  20% of sequence in fragments shorter than    3271 bp
  25% of sequence in fragments shorter than    3819 bp
  30% of sequence in fragments shorter than    4376 bp
  35% of sequence in fragments shorter than    4932 bp
  40% of sequence in fragments shorter than    5507 bp
  45% of sequence in fragments shorter than    6109 bp
  50% of sequence in fragments shorter than    6748 bp
  55% of sequence in fragments shorter than    7440 bp
  60% of sequence in fragments shorter than    8181 bp
  65% of sequence in fragments shorter than    9015 bp
  70% of sequence in fragments shorter than    9962 bp
  75% of sequence in fragments shorter than   11058 bp
  80% of sequence in fragments shorter than   12386 bp
  85% of sequence in fragments shorter than   14142 bp
  90% of sequence in fragments shorter than   16703 bp
  95% of sequence in fragments shorter than   22030 bp

# AclI.AvrII (AACGTT+CCTAGG) double digest, 767 thousand fragments (172706+594956 sites), 3883 bp average fragment length
   5% of sequence in fragments shorter than    1355 bp
  10% of sequence in fragments shorter than    2073 bp
  15% of sequence in fragments shorter than    2695 bp
  20% of sequence in fragments shorter than    3256 bp
  25% of sequence in fragments shorter than    3818 bp
  30% of sequence in fragments shorter than    4369 bp
  35% of sequence in fragments shorter than    4933 bp
  40% of sequence in fragments shorter than    5510 bp
  45% of sequence in fragments shorter than    6116 bp
  50% of sequence in fragments shorter than    6757 bp
  55% of sequence in fragments shorter than    7445 bp
  60% of sequence in fragments shorter than    8200 bp
  65% of sequence in fragments shorter than    9021 bp
  70% of sequence in fragments shorter than    9963 bp
  75% of sequence in fragments shorter than   11061 bp
  80% of sequence in fragments shorter than   12392 bp
  85% of sequence in fragments shorter than   14164 bp
  90% of sequence in fragments shorter than   16600 bp
  95% of sequence in fragments shorter than   21308 bp

# AgeI.BsaAI (ACCGGT+YACGTR) double digest, 766 thousand fragments (53386+713801 sites), 3886 bp average fragment length
   5% of sequence in fragments shorter than    1376 bp
  10% of sequence in fragments shorter than    2082 bp
  15% of sequence in fragments shorter than    2726 bp
  20% of sequence in fragments shorter than    3315 bp
  25% of sequence in fragments shorter than    3866 bp
  30% of sequence in fragments shorter than    4446 bp
  35% of sequence in fragments shorter than    5044 bp
  40% of sequence in fragments shorter than    5665 bp
  45% of sequence in fragments shorter than    6313 bp
  50% of sequence in fragments shorter than    7003 bp
  55% of sequence in fragments shorter than    7745 bp
  60% of sequence in fragments shorter than    8552 bp
  65% of sequence in fragments shorter than    9445 bp
  70% of sequence in fragments shorter than   10467 bp
  75% of sequence in fragments shorter than   11651 bp
  80% of sequence in fragments shorter than   13079 bp
  85% of sequence in fragments shorter than   14948 bp
  90% of sequence in fragments shorter than   17598 bp
  95% of sequence in fragments shorter than   22325 bp

# AatII.BciVI (GACGTC+GTATCC) double digest, 766 thousand fragments (71089+696093 sites), 3886 bp average fragment length
   5% of sequence in fragments shorter than    1383 bp
  10% of sequence in fragments shorter than    2057 bp
  15% of sequence in fragments shorter than    2650 bp
  20% of sequence in fragments shorter than    3222 bp
  25% of sequence in fragments shorter than    3773 bp
  30% of sequence in fragments shorter than    4317 bp
  35% of sequence in fragments shorter than    4875 bp
  40% of sequence in fragments shorter than    5449 bp
  45% of sequence in fragments shorter than    6041 bp
  50% of sequence in fragments shorter than    6675 bp
  55% of sequence in fragments shorter than    7347 bp
  60% of sequence in fragments shorter than    8092 bp
  65% of sequence in fragments shorter than    8908 bp
  70% of sequence in fragments shorter than    9840 bp
  75% of sequence in fragments shorter than   10924 bp
  80% of sequence in fragments shorter than   12212 bp
  85% of sequence in fragments shorter than   13908 bp
  90% of sequence in fragments shorter than   16369 bp
  95% of sequence in fragments shorter than   20746 bp

# HpaI.SapI (GTTAAC+GCTCTTC) double digest, 766 thousand fragments (389418+377161 sites), 3889 bp average fragment length
   5% of sequence in fragments shorter than    1358 bp
  10% of sequence in fragments shorter than    2062 bp
  15% of sequence in fragments shorter than    2673 bp
  20% of sequence in fragments shorter than    3244 bp
  25% of sequence in fragments shorter than    3794 bp
  30% of sequence in fragments shorter than    4341 bp
  35% of sequence in fragments shorter than    4902 bp
  40% of sequence in fragments shorter than    5473 bp
  45% of sequence in fragments shorter than    6074 bp
  50% of sequence in fragments shorter than    6712 bp
  55% of sequence in fragments shorter than    7398 bp
  60% of sequence in fragments shorter than    8142 bp
  65% of sequence in fragments shorter than    8968 bp
  70% of sequence in fragments shorter than    9904 bp
  75% of sequence in fragments shorter than   11015 bp
  80% of sequence in fragments shorter than   12373 bp
  85% of sequence in fragments shorter than   14107 bp
  90% of sequence in fragments shorter than   16718 bp
  95% of sequence in fragments shorter than   22115 bp

# HpaI.SmaI (GTTAAC+CCCGGG) double digest, 766 thousand fragments (389418+376940 sites), 3890 bp average fragment length
   5% of sequence in fragments shorter than    1323 bp
  10% of sequence in fragments shorter than    2051 bp
  15% of sequence in fragments shorter than    2690 bp
  20% of sequence in fragments shorter than    3302 bp
  25% of sequence in fragments shorter than    3895 bp
  30% of sequence in fragments shorter than    4494 bp
  35% of sequence in fragments shorter than    5114 bp
  40% of sequence in fragments shorter than    5762 bp
  45% of sequence in fragments shorter than    6455 bp
  50% of sequence in fragments shorter than    7181 bp
  55% of sequence in fragments shorter than    7976 bp
  60% of sequence in fragments shorter than    8848 bp
  65% of sequence in fragments shorter than    9813 bp
  70% of sequence in fragments shorter than   10925 bp
  75% of sequence in fragments shorter than   12243 bp
  80% of sequence in fragments shorter than   13877 bp
  85% of sequence in fragments shorter than   16020 bp
  90% of sequence in fragments shorter than   19186 bp
  95% of sequence in fragments shorter than   25645 bp

# BamHI.SpeI (GGATCC+ACTAGT) double digest, 765 thousand fragments (365999+400286 sites), 3890 bp average fragment length
   5% of sequence in fragments shorter than    1359 bp
  10% of sequence in fragments shorter than    2064 bp
  15% of sequence in fragments shorter than    2659 bp
  20% of sequence in fragments shorter than    3232 bp
  25% of sequence in fragments shorter than    3796 bp
  30% of sequence in fragments shorter than    4352 bp
  35% of sequence in fragments shorter than    4919 bp
  40% of sequence in fragments shorter than    5492 bp
  45% of sequence in fragments shorter than    6083 bp
  50% of sequence in fragments shorter than    6708 bp
  55% of sequence in fragments shorter than    7387 bp
  60% of sequence in fragments shorter than    8117 bp
  65% of sequence in fragments shorter than    8937 bp
  70% of sequence in fragments shorter than    9862 bp
  75% of sequence in fragments shorter than   10932 bp
  80% of sequence in fragments shorter than   12228 bp
  85% of sequence in fragments shorter than   13884 bp
  90% of sequence in fragments shorter than   16315 bp
  95% of sequence in fragments shorter than   20874 bp

# FnuDII.SalI (CGCG+GTCGAC) double digest, 764 thousand fragments (733940+31396 sites), 3895 bp average fragment length
   5% of sequence in fragments shorter than    1729 bp
  10% of sequence in fragments shorter than    3054 bp
  15% of sequence in fragments shorter than    4314 bp
  20% of sequence in fragments shorter than    5582 bp
  25% of sequence in fragments shorter than    6890 bp
  30% of sequence in fragments shorter than    8302 bp
  35% of sequence in fragments shorter than    9797 bp
  40% of sequence in fragments shorter than   11403 bp
  45% of sequence in fragments shorter than   13194 bp
  50% of sequence in fragments shorter than   15177 bp
  55% of sequence in fragments shorter than   17353 bp
  60% of sequence in fragments shorter than   19797 bp
  65% of sequence in fragments shorter than   22637 bp
  70% of sequence in fragments shorter than   25844 bp
  75% of sequence in fragments shorter than   29703 bp
  80% of sequence in fragments shorter than   34498 bp
  85% of sequence in fragments shorter than   41139 bp
  90% of sequence in fragments shorter than   50563 bp
  95% of sequence in fragments shorter than   69271 bp

# AflII.XhoI (CTTAAG+CTCGAG) double digest, 764 thousand fragments (643284+121308 sites), 3899 bp average fragment length
   5% of sequence in fragments shorter than    1382 bp
  10% of sequence in fragments shorter than    2080 bp
  15% of sequence in fragments shorter than    2648 bp
  20% of sequence in fragments shorter than    3184 bp
  25% of sequence in fragments shorter than    3727 bp
  30% of sequence in fragments shorter than    4269 bp
  35% of sequence in fragments shorter than    4820 bp
  40% of sequence in fragments shorter than    5384 bp
  45% of sequence in fragments shorter than    5974 bp
  50% of sequence in fragments shorter than    6605 bp
  55% of sequence in fragments shorter than    7280 bp
  60% of sequence in fragments shorter than    8014 bp
  65% of sequence in fragments shorter than    8832 bp
  70% of sequence in fragments shorter than    9756 bp
  75% of sequence in fragments shorter than   10846 bp
  80% of sequence in fragments shorter than   12175 bp
  85% of sequence in fragments shorter than   13904 bp
  90% of sequence in fragments shorter than   16471 bp
  95% of sequence in fragments shorter than   21825 bp

# AscI.NcoI (GGCGCGCC+CCATGG) double digest, 763 thousand fragments (4751+759594 sites), 3900 bp average fragment length
   5% of sequence in fragments shorter than    1354 bp
  10% of sequence in fragments shorter than    2069 bp
  15% of sequence in fragments shorter than    2705 bp
  20% of sequence in fragments shorter than    3309 bp
  25% of sequence in fragments shorter than    3903 bp
  30% of sequence in fragments shorter than    4491 bp
  35% of sequence in fragments shorter than    5072 bp
  40% of sequence in fragments shorter than    5678 bp
  45% of sequence in fragments shorter than    6317 bp
  50% of sequence in fragments shorter than    7017 bp
  55% of sequence in fragments shorter than    7774 bp
  60% of sequence in fragments shorter than    8610 bp
  65% of sequence in fragments shorter than    9533 bp
  70% of sequence in fragments shorter than   10597 bp
  75% of sequence in fragments shorter than   11865 bp
  80% of sequence in fragments shorter than   13385 bp
  85% of sequence in fragments shorter than   15384 bp
  90% of sequence in fragments shorter than   18376 bp
  95% of sequence in fragments shorter than   24352 bp

# BceAI.PspXI (ACGGC+VCTCGAGB) double digest, 763 thousand fragments (705178+59193 sites), 3900 bp average fragment length
   5% of sequence in fragments shorter than    1270 bp
  10% of sequence in fragments shorter than    2086 bp
  15% of sequence in fragments shorter than    2852 bp
  20% of sequence in fragments shorter than    3598 bp
  25% of sequence in fragments shorter than    4355 bp
  30% of sequence in fragments shorter than    5130 bp
  35% of sequence in fragments shorter than    5935 bp
  40% of sequence in fragments shorter than    6786 bp
  45% of sequence in fragments shorter than    7697 bp
  50% of sequence in fragments shorter than    8671 bp
  55% of sequence in fragments shorter than    9732 bp
  60% of sequence in fragments shorter than   10930 bp
  65% of sequence in fragments shorter than   12269 bp
  70% of sequence in fragments shorter than   13809 bp
  75% of sequence in fragments shorter than   15606 bp
  80% of sequence in fragments shorter than   17824 bp
  85% of sequence in fragments shorter than   20712 bp
  90% of sequence in fragments shorter than   24985 bp
  95% of sequence in fragments shorter than   32891 bp

# BciVI.SacII (GTATCC+CCGCGG) double digest, 762 thousand fragments (696093+66901 sites), 3907 bp average fragment length
   5% of sequence in fragments shorter than    1400 bp
  10% of sequence in fragments shorter than    2092 bp
  15% of sequence in fragments shorter than    2727 bp
  20% of sequence in fragments shorter than    3320 bp
  25% of sequence in fragments shorter than    3896 bp
  30% of sequence in fragments shorter than    4460 bp
  35% of sequence in fragments shorter than    5038 bp
  40% of sequence in fragments shorter than    5632 bp
  45% of sequence in fragments shorter than    6253 bp
  50% of sequence in fragments shorter than    6909 bp
  55% of sequence in fragments shorter than    7608 bp
  60% of sequence in fragments shorter than    8384 bp
  65% of sequence in fragments shorter than    9237 bp
  70% of sequence in fragments shorter than   10205 bp
  75% of sequence in fragments shorter than   11323 bp
  80% of sequence in fragments shorter than   12688 bp
  85% of sequence in fragments shorter than   14450 bp
  90% of sequence in fragments shorter than   16980 bp
  95% of sequence in fragments shorter than   21628 bp

# PacI.SacI (TTAATTAA+GAGCTC) double digest, 762 thousand fragments (159455+603323 sites), 3908 bp average fragment length
   5% of sequence in fragments shorter than    1373 bp
  10% of sequence in fragments shorter than    2086 bp
  15% of sequence in fragments shorter than    2702 bp
  20% of sequence in fragments shorter than    3288 bp
  25% of sequence in fragments shorter than    3870 bp
  30% of sequence in fragments shorter than    4444 bp
  35% of sequence in fragments shorter than    5027 bp
  40% of sequence in fragments shorter than    5632 bp
  45% of sequence in fragments shorter than    6264 bp
  50% of sequence in fragments shorter than    6936 bp
  55% of sequence in fragments shorter than    7666 bp
  60% of sequence in fragments shorter than    8445 bp
  65% of sequence in fragments shorter than    9320 bp
  70% of sequence in fragments shorter than   10326 bp
  75% of sequence in fragments shorter than   11496 bp
  80% of sequence in fragments shorter than   12895 bp
  85% of sequence in fragments shorter than   14712 bp
  90% of sequence in fragments shorter than   17343 bp
  95% of sequence in fragments shorter than   22699 bp

# HaeII.SnaBI (RGCGCY+TACGTA) double digest, 761 thousand fragments (630101+132299 sites), 3911 bp average fragment length
   5% of sequence in fragments shorter than    1211 bp
  10% of sequence in fragments shorter than    2162 bp
  15% of sequence in fragments shorter than    3048 bp
  20% of sequence in fragments shorter than    3899 bp
  25% of sequence in fragments shorter than    4736 bp
  30% of sequence in fragments shorter than    5594 bp
  35% of sequence in fragments shorter than    6482 bp
  40% of sequence in fragments shorter than    7411 bp
  45% of sequence in fragments shorter than    8401 bp
  50% of sequence in fragments shorter than    9473 bp
  55% of sequence in fragments shorter than   10632 bp
  60% of sequence in fragments shorter than   11908 bp
  65% of sequence in fragments shorter than   13340 bp
  70% of sequence in fragments shorter than   14994 bp
  75% of sequence in fragments shorter than   16939 bp
  80% of sequence in fragments shorter than   19295 bp
  85% of sequence in fragments shorter than   22365 bp
  90% of sequence in fragments shorter than   26569 bp
  95% of sequence in fragments shorter than   33949 bp

# Hpy99I.SacI (CGWCG+GAGCTC) double digest, 760 thousand fragments (157917+603323 sites), 3916 bp average fragment length
   5% of sequence in fragments shorter than    1333 bp
  10% of sequence in fragments shorter than    2075 bp
  15% of sequence in fragments shorter than    2751 bp
  20% of sequence in fragments shorter than    3408 bp
  25% of sequence in fragments shorter than    4065 bp
  30% of sequence in fragments shorter than    4718 bp
  35% of sequence in fragments shorter than    5389 bp
  40% of sequence in fragments shorter than    6101 bp
  45% of sequence in fragments shorter than    6852 bp
  50% of sequence in fragments shorter than    7652 bp
  55% of sequence in fragments shorter than    8511 bp
  60% of sequence in fragments shorter than    9475 bp
  65% of sequence in fragments shorter than   10568 bp
  70% of sequence in fragments shorter than   11797 bp
  75% of sequence in fragments shorter than   13261 bp
  80% of sequence in fragments shorter than   15005 bp
  85% of sequence in fragments shorter than   17324 bp
  90% of sequence in fragments shorter than   20770 bp
  95% of sequence in fragments shorter than   27553 bp

# BclI.MluI (TGATCA+ACGCGT) double digest, 759 thousand fragments (738785+21415 sites), 3921 bp average fragment length
   5% of sequence in fragments shorter than    1387 bp
  10% of sequence in fragments shorter than    2075 bp
  15% of sequence in fragments shorter than    2663 bp
  20% of sequence in fragments shorter than    3226 bp
  25% of sequence in fragments shorter than    3780 bp
  30% of sequence in fragments shorter than    4319 bp
  35% of sequence in fragments shorter than    4873 bp
  40% of sequence in fragments shorter than    5440 bp
  45% of sequence in fragments shorter than    6035 bp
  50% of sequence in fragments shorter than    6670 bp
  55% of sequence in fragments shorter than    7349 bp
  60% of sequence in fragments shorter than    8088 bp
  65% of sequence in fragments shorter than    8907 bp
  70% of sequence in fragments shorter than    9852 bp
  75% of sequence in fragments shorter than   10960 bp
  80% of sequence in fragments shorter than   12302 bp
  85% of sequence in fragments shorter than   14048 bp
  90% of sequence in fragments shorter than   16656 bp
  95% of sequence in fragments shorter than   22074 bp

# AgeI.BceAI (ACCGGT+ACGGC) double digest, 758 thousand fragments (53386+705178 sites), 3930 bp average fragment length
   5% of sequence in fragments shorter than    1277 bp
  10% of sequence in fragments shorter than    2095 bp
  15% of sequence in fragments shorter than    2867 bp
  20% of sequence in fragments shorter than    3610 bp
  25% of sequence in fragments shorter than    4362 bp
  30% of sequence in fragments shorter than    5132 bp
  35% of sequence in fragments shorter than    5930 bp
  40% of sequence in fragments shorter than    6767 bp
  45% of sequence in fragments shorter than    7668 bp
  50% of sequence in fragments shorter than    8624 bp
  55% of sequence in fragments shorter than    9677 bp
  60% of sequence in fragments shorter than   10858 bp
  65% of sequence in fragments shorter than   12177 bp
  70% of sequence in fragments shorter than   13681 bp
  75% of sequence in fragments shorter than   15480 bp
  80% of sequence in fragments shorter than   17650 bp
  85% of sequence in fragments shorter than   20486 bp
  90% of sequence in fragments shorter than   24582 bp
  95% of sequence in fragments shorter than   32307 bp

# ApaI.SexAI (GGGCCC+ACCWGGT) double digest, 757 thousand fragments (460339+297900 sites), 3931 bp average fragment length
   5% of sequence in fragments shorter than    1249 bp
  10% of sequence in fragments shorter than    2061 bp
  15% of sequence in fragments shorter than    2821 bp
  20% of sequence in fragments shorter than    3578 bp
  25% of sequence in fragments shorter than    4343 bp
  30% of sequence in fragments shorter than    5127 bp
  35% of sequence in fragments shorter than    5953 bp
  40% of sequence in fragments shorter than    6815 bp
  45% of sequence in fragments shorter than    7739 bp
  50% of sequence in fragments shorter than    8720 bp
  55% of sequence in fragments shorter than    9787 bp
  60% of sequence in fragments shorter than   10977 bp
  65% of sequence in fragments shorter than   12315 bp
  70% of sequence in fragments shorter than   13822 bp
  75% of sequence in fragments shorter than   15619 bp
  80% of sequence in fragments shorter than   17871 bp
  85% of sequence in fragments shorter than   20842 bp
  90% of sequence in fragments shorter than   25281 bp
  95% of sequence in fragments shorter than   35023 bp

# BsaAI.PmeI (YACGTR+GTTTAAAC) double digest, 755 thousand fragments (713801+42094 sites), 3944 bp average fragment length
   5% of sequence in fragments shorter than    1385 bp
  10% of sequence in fragments shorter than    2107 bp
  15% of sequence in fragments shorter than    2759 bp
  20% of sequence in fragments shorter than    3350 bp
  25% of sequence in fragments shorter than    3910 bp
  30% of sequence in fragments shorter than    4488 bp
  35% of sequence in fragments shorter than    5089 bp
  40% of sequence in fragments shorter than    5708 bp
  45% of sequence in fragments shorter than    6357 bp
  50% of sequence in fragments shorter than    7041 bp
  55% of sequence in fragments shorter than    7776 bp
  60% of sequence in fragments shorter than    8585 bp
  65% of sequence in fragments shorter than    9480 bp
  70% of sequence in fragments shorter than   10488 bp
  75% of sequence in fragments shorter than   11668 bp
  80% of sequence in fragments shorter than   13077 bp
  85% of sequence in fragments shorter than   14919 bp
  90% of sequence in fragments shorter than   17518 bp
  95% of sequence in fragments shorter than   22179 bp

# HaeII.NaeI (RGCGCY+GCCGGC) double digest, 755 thousand fragments (630101+125692 sites), 3945 bp average fragment length
   5% of sequence in fragments shorter than    1202 bp
  10% of sequence in fragments shorter than    2245 bp
  15% of sequence in fragments shorter than    3297 bp
  20% of sequence in fragments shorter than    4333 bp
  25% of sequence in fragments shorter than    5412 bp
  30% of sequence in fragments shorter than    6517 bp
  35% of sequence in fragments shorter than    7703 bp
  40% of sequence in fragments shorter than    8977 bp
  45% of sequence in fragments shorter than   10331 bp
  50% of sequence in fragments shorter than   11821 bp
  55% of sequence in fragments shorter than   13501 bp
  60% of sequence in fragments shorter than   15355 bp
  65% of sequence in fragments shorter than   17438 bp
  70% of sequence in fragments shorter than   19918 bp
  75% of sequence in fragments shorter than   22821 bp
  80% of sequence in fragments shorter than   26359 bp
  85% of sequence in fragments shorter than   31014 bp
  90% of sequence in fragments shorter than   37350 bp
  95% of sequence in fragments shorter than   49039 bp

# BamHI.HpaI (GGATCC+GTTAAC) double digest, 755 thousand fragments (365999+389418 sites), 3946 bp average fragment length
   5% of sequence in fragments shorter than    1405 bp
  10% of sequence in fragments shorter than    2092 bp
  15% of sequence in fragments shorter than    2673 bp
  20% of sequence in fragments shorter than    3238 bp
  25% of sequence in fragments shorter than    3793 bp
  30% of sequence in fragments shorter than    4341 bp
  35% of sequence in fragments shorter than    4903 bp
  40% of sequence in fragments shorter than    5485 bp
  45% of sequence in fragments shorter than    6085 bp
  50% of sequence in fragments shorter than    6711 bp
  55% of sequence in fragments shorter than    7395 bp
  60% of sequence in fragments shorter than    8144 bp
  65% of sequence in fragments shorter than    8953 bp
  70% of sequence in fragments shorter than    9885 bp
  75% of sequence in fragments shorter than   10963 bp
  80% of sequence in fragments shorter than   12287 bp
  85% of sequence in fragments shorter than   13972 bp
  90% of sequence in fragments shorter than   16441 bp
  95% of sequence in fragments shorter than   21178 bp

# FnuDII.MluI (CGCG+ACGCGT) double digest, 754 thousand fragments (733940+21415 sites), 3947 bp average fragment length
   5% of sequence in fragments shorter than    1828 bp
  10% of sequence in fragments shorter than    3245 bp
  15% of sequence in fragments shorter than    4614 bp
  20% of sequence in fragments shorter than    6000 bp
  25% of sequence in fragments shorter than    7451 bp
  30% of sequence in fragments shorter than    8999 bp
  35% of sequence in fragments shorter than   10671 bp
  40% of sequence in fragments shorter than   12471 bp
  45% of sequence in fragments shorter than   14502 bp
  50% of sequence in fragments shorter than   16747 bp
  55% of sequence in fragments shorter than   19236 bp
  60% of sequence in fragments shorter than   22038 bp
  65% of sequence in fragments shorter than   25222 bp
  70% of sequence in fragments shorter than   28949 bp
  75% of sequence in fragments shorter than   33422 bp
  80% of sequence in fragments shorter than   39112 bp
  85% of sequence in fragments shorter than   46701 bp
  90% of sequence in fragments shorter than   57740 bp
  95% of sequence in fragments shorter than   79981 bp

# BciVI.PspXI (GTATCC+VCTCGAGB) double digest, 754 thousand fragments (696093+59193 sites), 3947 bp average fragment length
   5% of sequence in fragments shorter than    1404 bp
  10% of sequence in fragments shorter than    2084 bp
  15% of sequence in fragments shorter than    2692 bp
  20% of sequence in fragments shorter than    3269 bp
  25% of sequence in fragments shorter than    3825 bp
  30% of sequence in fragments shorter than    4375 bp
  35% of sequence in fragments shorter than    4937 bp
  40% of sequence in fragments shorter than    5524 bp
  45% of sequence in fragments shorter than    6132 bp
  50% of sequence in fragments shorter than    6772 bp
  55% of sequence in fragments shorter than    7459 bp
  60% of sequence in fragments shorter than    8219 bp
  65% of sequence in fragments shorter than    9045 bp
  70% of sequence in fragments shorter than   10000 bp
  75% of sequence in fragments shorter than   11096 bp
  80% of sequence in fragments shorter than   12428 bp
  85% of sequence in fragments shorter than   14140 bp
  90% of sequence in fragments shorter than   16634 bp
  95% of sequence in fragments shorter than   21207 bp

# AvrII.PacI (CCTAGG+TTAATTAA) double digest, 754 thousand fragments (594956+159455 sites), 3951 bp average fragment length
   5% of sequence in fragments shorter than    1401 bp
  10% of sequence in fragments shorter than    2138 bp
  15% of sequence in fragments shorter than    2766 bp
  20% of sequence in fragments shorter than    3353 bp
  25% of sequence in fragments shorter than    3921 bp
  30% of sequence in fragments shorter than    4486 bp
  35% of sequence in fragments shorter than    5055 bp
  40% of sequence in fragments shorter than    5636 bp
  45% of sequence in fragments shorter than    6248 bp
  50% of sequence in fragments shorter than    6894 bp
  55% of sequence in fragments shorter than    7594 bp
  60% of sequence in fragments shorter than    8347 bp
  65% of sequence in fragments shorter than    9199 bp
  70% of sequence in fragments shorter than   10152 bp
  75% of sequence in fragments shorter than   11267 bp
  80% of sequence in fragments shorter than   12636 bp
  85% of sequence in fragments shorter than   14404 bp
  90% of sequence in fragments shorter than   17022 bp
  95% of sequence in fragments shorter than   22408 bp

# SapI.SmaI (GCTCTTC+CCCGGG) double digest, 753 thousand fragments (377161+376940 sites), 3953 bp average fragment length
   5% of sequence in fragments shorter than    1302 bp
  10% of sequence in fragments shorter than    2053 bp
  15% of sequence in fragments shorter than    2733 bp
  20% of sequence in fragments shorter than    3389 bp
  25% of sequence in fragments shorter than    4046 bp
  30% of sequence in fragments shorter than    4710 bp
  35% of sequence in fragments shorter than    5392 bp
  40% of sequence in fragments shorter than    6114 bp
  45% of sequence in fragments shorter than    6892 bp
  50% of sequence in fragments shorter than    7719 bp
  55% of sequence in fragments shorter than    8624 bp
  60% of sequence in fragments shorter than    9620 bp
  65% of sequence in fragments shorter than   10759 bp
  70% of sequence in fragments shorter than   12051 bp
  75% of sequence in fragments shorter than   13593 bp
  80% of sequence in fragments shorter than   15519 bp
  85% of sequence in fragments shorter than   18024 bp
  90% of sequence in fragments shorter than   21834 bp
  95% of sequence in fragments shorter than   29937 bp

# BclI.SgrAI (TGATCA+CRCCGGYG) double digest, 753 thousand fragments (738785+15270 sites), 3953 bp average fragment length
   5% of sequence in fragments shorter than    1398 bp
  10% of sequence in fragments shorter than    2092 bp
  15% of sequence in fragments shorter than    2684 bp
  20% of sequence in fragments shorter than    3251 bp
  25% of sequence in fragments shorter than    3810 bp
  30% of sequence in fragments shorter than    4352 bp
  35% of sequence in fragments shorter than    4909 bp
  40% of sequence in fragments shorter than    5476 bp
  45% of sequence in fragments shorter than    6078 bp
  50% of sequence in fragments shorter than    6717 bp
  55% of sequence in fragments shorter than    7398 bp
  60% of sequence in fragments shorter than    8143 bp
  65% of sequence in fragments shorter than    8969 bp
  70% of sequence in fragments shorter than    9924 bp
  75% of sequence in fragments shorter than   11047 bp
  80% of sequence in fragments shorter than   12395 bp
  85% of sequence in fragments shorter than   14172 bp
  90% of sequence in fragments shorter than   16805 bp
  95% of sequence in fragments shorter than   22299 bp

# BclI.NruI (TGATCA+TCGCGA) double digest, 753 thousand fragments (738785+14872 sites), 3955 bp average fragment length
   5% of sequence in fragments shorter than    1398 bp
  10% of sequence in fragments shorter than    2090 bp
  15% of sequence in fragments shorter than    2684 bp
  20% of sequence in fragments shorter than    3251 bp
  25% of sequence in fragments shorter than    3811 bp
  30% of sequence in fragments shorter than    4351 bp
  35% of sequence in fragments shorter than    4909 bp
  40% of sequence in fragments shorter than    5479 bp
  45% of sequence in fragments shorter than    6085 bp
  50% of sequence in fragments shorter than    6724 bp
  55% of sequence in fragments shorter than    7407 bp
  60% of sequence in fragments shorter than    8157 bp
  65% of sequence in fragments shorter than    8987 bp
  70% of sequence in fragments shorter than    9952 bp
  75% of sequence in fragments shorter than   11075 bp
  80% of sequence in fragments shorter than   12430 bp
  85% of sequence in fragments shorter than   14215 bp
  90% of sequence in fragments shorter than   16915 bp
  95% of sequence in fragments shorter than   22483 bp

# BclI.PvuI (TGATCA+CGATCG) double digest, 752 thousand fragments (738785+14186 sites), 3959 bp average fragment length
   5% of sequence in fragments shorter than    1400 bp
  10% of sequence in fragments shorter than    2087 bp
  15% of sequence in fragments shorter than    2683 bp
  20% of sequence in fragments shorter than    3253 bp
  25% of sequence in fragments shorter than    3814 bp
  30% of sequence in fragments shorter than    4357 bp
  35% of sequence in fragments shorter than    4919 bp
  40% of sequence in fragments shorter than    5491 bp
  45% of sequence in fragments shorter than    6096 bp
  50% of sequence in fragments shorter than    6735 bp
  55% of sequence in fragments shorter than    7420 bp
  60% of sequence in fragments shorter than    8168 bp
  65% of sequence in fragments shorter than    9002 bp
  70% of sequence in fragments shorter than    9969 bp
  75% of sequence in fragments shorter than   11097 bp
  80% of sequence in fragments shorter than   12464 bp
  85% of sequence in fragments shorter than   14258 bp
  90% of sequence in fragments shorter than   16940 bp
  95% of sequence in fragments shorter than   22462 bp

# AvrII.Hpy99I (CCTAGG+CGWCG) double digest, 752 thousand fragments (594956+157917 sites), 3960 bp average fragment length
   5% of sequence in fragments shorter than    1367 bp
  10% of sequence in fragments shorter than    2143 bp
  15% of sequence in fragments shorter than    2809 bp
  20% of sequence in fragments shorter than    3442 bp
  25% of sequence in fragments shorter than    4051 bp
  30% of sequence in fragments shorter than    4634 bp
  35% of sequence in fragments shorter than    5262 bp
  40% of sequence in fragments shorter than    5908 bp
  45% of sequence in fragments shorter than    6596 bp
  50% of sequence in fragments shorter than    7325 bp
  55% of sequence in fragments shorter than    8113 bp
  60% of sequence in fragments shorter than    8973 bp
  65% of sequence in fragments shorter than    9941 bp
  70% of sequence in fragments shorter than   11046 bp
  75% of sequence in fragments shorter than   12333 bp
  80% of sequence in fragments shorter than   13959 bp
  85% of sequence in fragments shorter than   16044 bp
  90% of sequence in fragments shorter than   19085 bp
  95% of sequence in fragments shorter than   25386 bp

# BclI.FseI (TGATCA+GGCCGGCC) double digest, 752 thousand fragments (738785+13662 sites), 3962 bp average fragment length
   5% of sequence in fragments shorter than    1402 bp
  10% of sequence in fragments shorter than    2095 bp
  15% of sequence in fragments shorter than    2690 bp
  20% of sequence in fragments shorter than    3259 bp
  25% of sequence in fragments shorter than    3819 bp
  30% of sequence in fragments shorter than    4361 bp
  35% of sequence in fragments shorter than    4918 bp
  40% of sequence in fragments shorter than    5490 bp
  45% of sequence in fragments shorter than    6091 bp
  50% of sequence in fragments shorter than    6730 bp
  55% of sequence in fragments shorter than    7416 bp
  60% of sequence in fragments shorter than    8162 bp
  65% of sequence in fragments shorter than    8989 bp
  70% of sequence in fragments shorter than    9944 bp
  75% of sequence in fragments shorter than   11062 bp
  80% of sequence in fragments shorter than   12403 bp
  85% of sequence in fragments shorter than   14174 bp
  90% of sequence in fragments shorter than   16811 bp
  95% of sequence in fragments shorter than   22269 bp

# HaeII.XhoI (RGCGCY+CTCGAG) double digest, 750 thousand fragments (630101+121308 sites), 3968 bp average fragment length
   5% of sequence in fragments shorter than    1185 bp
  10% of sequence in fragments shorter than    2153 bp
  15% of sequence in fragments shorter than    3104 bp
  20% of sequence in fragments shorter than    4026 bp
  25% of sequence in fragments shorter than    4971 bp
  30% of sequence in fragments shorter than    5946 bp
  35% of sequence in fragments shorter than    6968 bp
  40% of sequence in fragments shorter than    8057 bp
  45% of sequence in fragments shorter than    9235 bp
  50% of sequence in fragments shorter than   10504 bp
  55% of sequence in fragments shorter than   11915 bp
  60% of sequence in fragments shorter than   13501 bp
  65% of sequence in fragments shorter than   15307 bp
  70% of sequence in fragments shorter than   17368 bp
  75% of sequence in fragments shorter than   19839 bp
  80% of sequence in fragments shorter than   22857 bp
  85% of sequence in fragments shorter than   26669 bp
  90% of sequence in fragments shorter than   32189 bp
  95% of sequence in fragments shorter than   41818 bp

# AgeI.BciVI (ACCGGT+GTATCC) double digest, 749 thousand fragments (53386+696093 sites), 3978 bp average fragment length
   5% of sequence in fragments shorter than    1412 bp
  10% of sequence in fragments shorter than    2091 bp
  15% of sequence in fragments shorter than    2703 bp
  20% of sequence in fragments shorter than    3285 bp
  25% of sequence in fragments shorter than    3856 bp
  30% of sequence in fragments shorter than    4415 bp
  35% of sequence in fragments shorter than    4990 bp
  40% of sequence in fragments shorter than    5587 bp
  45% of sequence in fragments shorter than    6207 bp
  50% of sequence in fragments shorter than    6853 bp
  55% of sequence in fragments shorter than    7556 bp
  60% of sequence in fragments shorter than    8327 bp
  65% of sequence in fragments shorter than    9171 bp
  70% of sequence in fragments shorter than   10139 bp
  75% of sequence in fragments shorter than   11257 bp
  80% of sequence in fragments shorter than   12614 bp
  85% of sequence in fragments shorter than   14389 bp
  90% of sequence in fragments shorter than   16949 bp
  95% of sequence in fragments shorter than   21802 bp

# FnuDII.SgrAI (CGCG+CRCCGGYG) double digest, 748 thousand fragments (733940+15270 sites), 3979 bp average fragment length
   5% of sequence in fragments shorter than    1792 bp
  10% of sequence in fragments shorter than    3188 bp
  15% of sequence in fragments shorter than    4533 bp
  20% of sequence in fragments shorter than    5888 bp
  25% of sequence in fragments shorter than    7317 bp
  30% of sequence in fragments shorter than    8843 bp
  35% of sequence in fragments shorter than   10483 bp
  40% of sequence in fragments shorter than   12257 bp
  45% of sequence in fragments shorter than   14236 bp
  50% of sequence in fragments shorter than   16434 bp
  55% of sequence in fragments shorter than   18900 bp
  60% of sequence in fragments shorter than   21627 bp
  65% of sequence in fragments shorter than   24770 bp
  70% of sequence in fragments shorter than   28390 bp
  75% of sequence in fragments shorter than   32750 bp
  80% of sequence in fragments shorter than   38320 bp
  85% of sequence in fragments shorter than   45819 bp
  90% of sequence in fragments shorter than   56626 bp
  95% of sequence in fragments shorter than   78124 bp

# FnuDII.NruI (CGCG+TCGCGA) double digest, 748 thousand fragments (733940+14872 sites), 3981 bp average fragment length
   5% of sequence in fragments shorter than    1828 bp
  10% of sequence in fragments shorter than    3245 bp
  15% of sequence in fragments shorter than    4614 bp
  20% of sequence in fragments shorter than    6000 bp
  25% of sequence in fragments shorter than    7451 bp
  30% of sequence in fragments shorter than    8999 bp
  35% of sequence in fragments shorter than   10672 bp
  40% of sequence in fragments shorter than   12471 bp
  45% of sequence in fragments shorter than   14502 bp
  50% of sequence in fragments shorter than   16747 bp
  55% of sequence in fragments shorter than   19236 bp
  60% of sequence in fragments shorter than   22038 bp
  65% of sequence in fragments shorter than   25222 bp
  70% of sequence in fragments shorter than   28949 bp
  75% of sequence in fragments shorter than   33422 bp
  80% of sequence in fragments shorter than   39112 bp
  85% of sequence in fragments shorter than   46701 bp
  90% of sequence in fragments shorter than   57740 bp
  95% of sequence in fragments shorter than   79981 bp

# BclI.NotI (TGATCA+GCGGCCGC) double digest, 748 thousand fragments (738785+9795 sites), 3982 bp average fragment length
   5% of sequence in fragments shorter than    1408 bp
  10% of sequence in fragments shorter than    2107 bp
  15% of sequence in fragments shorter than    2704 bp
  20% of sequence in fragments shorter than    3277 bp
  25% of sequence in fragments shorter than    3840 bp
  30% of sequence in fragments shorter than    4386 bp
  35% of sequence in fragments shorter than    4949 bp
  40% of sequence in fragments shorter than    5523 bp
  45% of sequence in fragments shorter than    6132 bp
  50% of sequence in fragments shorter than    6772 bp
  55% of sequence in fragments shorter than    7461 bp
  60% of sequence in fragments shorter than    8214 bp
  65% of sequence in fragments shorter than    9048 bp
  70% of sequence in fragments shorter than   10017 bp
  75% of sequence in fragments shorter than   11150 bp
  80% of sequence in fragments shorter than   12507 bp
  85% of sequence in fragments shorter than   14309 bp
  90% of sequence in fragments shorter than   17008 bp
  95% of sequence in fragments shorter than   22613 bp

# BclI.RsrII (TGATCA+CGGWCCG) double digest, 748 thousand fragments (738785+9764 sites), 3982 bp average fragment length
   5% of sequence in fragments shorter than    1408 bp
  10% of sequence in fragments shorter than    2105 bp
  15% of sequence in fragments shorter than    2702 bp
  20% of sequence in fragments shorter than    3274 bp
  25% of sequence in fragments shorter than    3837 bp
  30% of sequence in fragments shorter than    4383 bp
  35% of sequence in fragments shorter than    4945 bp
  40% of sequence in fragments shorter than    5519 bp
  45% of sequence in fragments shorter than    6129 bp
  50% of sequence in fragments shorter than    6771 bp
  55% of sequence in fragments shorter than    7464 bp
  60% of sequence in fragments shorter than    8215 bp
  65% of sequence in fragments shorter than    9050 bp
  70% of sequence in fragments shorter than   10025 bp
  75% of sequence in fragments shorter than   11152 bp
  80% of sequence in fragments shorter than   12516 bp
  85% of sequence in fragments shorter than   14318 bp
  90% of sequence in fragments shorter than   17004 bp
  95% of sequence in fragments shorter than   22590 bp

# ApaI.KpnI (GGGCCC+GGTACC) double digest, 748 thousand fragments (460339+287974 sites), 3983 bp average fragment length
   5% of sequence in fragments shorter than    1261 bp
  10% of sequence in fragments shorter than    2042 bp
  15% of sequence in fragments shorter than    2812 bp
  20% of sequence in fragments shorter than    3550 bp
  25% of sequence in fragments shorter than    4304 bp
  30% of sequence in fragments shorter than    5073 bp
  35% of sequence in fragments shorter than    5880 bp
  40% of sequence in fragments shorter than    6721 bp
  45% of sequence in fragments shorter than    7610 bp
  50% of sequence in fragments shorter than    8565 bp
  55% of sequence in fragments shorter than    9593 bp
  60% of sequence in fragments shorter than   10715 bp
  65% of sequence in fragments shorter than   11996 bp
  70% of sequence in fragments shorter than   13471 bp
  75% of sequence in fragments shorter than   15200 bp
  80% of sequence in fragments shorter than   17335 bp
  85% of sequence in fragments shorter than   20196 bp
  90% of sequence in fragments shorter than   24400 bp
  95% of sequence in fragments shorter than   33224 bp

# FnuDII.PvuI (CGCG+CGATCG) double digest, 747 thousand fragments (733940+14186 sites), 3985 bp average fragment length
   5% of sequence in fragments shorter than    1779 bp
  10% of sequence in fragments shorter than    3155 bp
  15% of sequence in fragments shorter than    4484 bp
  20% of sequence in fragments shorter than    5832 bp
  25% of sequence in fragments shorter than    7240 bp
  30% of sequence in fragments shorter than    8744 bp
  35% of sequence in fragments shorter than   10368 bp
  40% of sequence in fragments shorter than   12106 bp
  45% of sequence in fragments shorter than   14065 bp
  50% of sequence in fragments shorter than   16233 bp
  55% of sequence in fragments shorter than   18643 bp
  60% of sequence in fragments shorter than   21365 bp
  65% of sequence in fragments shorter than   24482 bp
  70% of sequence in fragments shorter than   28038 bp
  75% of sequence in fragments shorter than   32309 bp
  80% of sequence in fragments shorter than   37741 bp
  85% of sequence in fragments shorter than   45061 bp
  90% of sequence in fragments shorter than   55506 bp
  95% of sequence in fragments shorter than   76496 bp

# ApaI.NheI (GGGCCC+GCTAGC) double digest, 747 thousand fragments (460339+287384 sites), 3987 bp average fragment length
   5% of sequence in fragments shorter than    1346 bp
  10% of sequence in fragments shorter than    2073 bp
  15% of sequence in fragments shorter than    2781 bp
  20% of sequence in fragments shorter than    3504 bp
  25% of sequence in fragments shorter than    4250 bp
  30% of sequence in fragments shorter than    5003 bp
  35% of sequence in fragments shorter than    5785 bp
  40% of sequence in fragments shorter than    6610 bp
  45% of sequence in fragments shorter than    7479 bp
  50% of sequence in fragments shorter than    8410 bp
  55% of sequence in fragments shorter than    9423 bp
  60% of sequence in fragments shorter than   10529 bp
  65% of sequence in fragments shorter than   11779 bp
  70% of sequence in fragments shorter than   13205 bp
  75% of sequence in fragments shorter than   14900 bp
  80% of sequence in fragments shorter than   16973 bp
  85% of sequence in fragments shorter than   19606 bp
  90% of sequence in fragments shorter than   23545 bp
  95% of sequence in fragments shorter than   31653 bp

# FnuDII.FseI (CGCG+GGCCGGCC) double digest, 747 thousand fragments (733940+13662 sites), 3988 bp average fragment length
   5% of sequence in fragments shorter than    1785 bp
  10% of sequence in fragments shorter than    3182 bp
  15% of sequence in fragments shorter than    4531 bp
  20% of sequence in fragments shorter than    5898 bp
  25% of sequence in fragments shorter than    7329 bp
  30% of sequence in fragments shorter than    8868 bp
  35% of sequence in fragments shorter than   10520 bp
  40% of sequence in fragments shorter than   12307 bp
  45% of sequence in fragments shorter than   14305 bp
  50% of sequence in fragments shorter than   16533 bp
  55% of sequence in fragments shorter than   18996 bp
  60% of sequence in fragments shorter than   21759 bp
  65% of sequence in fragments shorter than   24942 bp
  70% of sequence in fragments shorter than   28647 bp
  75% of sequence in fragments shorter than   33036 bp
  80% of sequence in fragments shorter than   38633 bp
  85% of sequence in fragments shorter than   46205 bp
  90% of sequence in fragments shorter than   57094 bp
  95% of sequence in fragments shorter than   78869 bp

# BceAI.PmeI (ACGGC+GTTTAAAC) double digest, 746 thousand fragments (705178+42094 sites), 3990 bp average fragment length
   5% of sequence in fragments shorter than    1312 bp
  10% of sequence in fragments shorter than    2141 bp
  15% of sequence in fragments shorter than    2905 bp
  20% of sequence in fragments shorter than    3645 bp
  25% of sequence in fragments shorter than    4392 bp
  30% of sequence in fragments shorter than    5152 bp
  35% of sequence in fragments shorter than    5936 bp
  40% of sequence in fragments shorter than    6760 bp
  45% of sequence in fragments shorter than    7641 bp
  50% of sequence in fragments shorter than    8590 bp
  55% of sequence in fragments shorter than    9612 bp
  60% of sequence in fragments shorter than   10758 bp
  65% of sequence in fragments shorter than   12047 bp
  70% of sequence in fragments shorter than   13497 bp
  75% of sequence in fragments shorter than   15228 bp
  80% of sequence in fragments shorter than   17322 bp
  85% of sequence in fragments shorter than   20075 bp
  90% of sequence in fragments shorter than   24075 bp
  95% of sequence in fragments shorter than   31456 bp

# BsaAI.SalI (YACGTR+GTCGAC) double digest, 744 thousand fragments (713801+31396 sites), 4001 bp average fragment length
   5% of sequence in fragments shorter than    1400 bp
  10% of sequence in fragments shorter than    2139 bp
  15% of sequence in fragments shorter than    2795 bp
  20% of sequence in fragments shorter than    3404 bp
  25% of sequence in fragments shorter than    3976 bp
  30% of sequence in fragments shorter than    4567 bp
  35% of sequence in fragments shorter than    5181 bp
  40% of sequence in fragments shorter than    5815 bp
  45% of sequence in fragments shorter than    6478 bp
  50% of sequence in fragments shorter than    7176 bp
  55% of sequence in fragments shorter than    7937 bp
  60% of sequence in fragments shorter than    8762 bp
  65% of sequence in fragments shorter than    9678 bp
  70% of sequence in fragments shorter than   10720 bp
  75% of sequence in fragments shorter than   11928 bp
  80% of sequence in fragments shorter than   13401 bp
  85% of sequence in fragments shorter than   15320 bp
  90% of sequence in fragments shorter than   18027 bp
  95% of sequence in fragments shorter than   22865 bp

# AclI.HgaI (AACGTT+GACGC) double digest, 744 thousand fragments (172706+571889 sites), 4004 bp average fragment length
   5% of sequence in fragments shorter than    1335 bp
  10% of sequence in fragments shorter than    2161 bp
  15% of sequence in fragments shorter than    2950 bp
  20% of sequence in fragments shorter than    3684 bp
  25% of sequence in fragments shorter than    4430 bp
  30% of sequence in fragments shorter than    5193 bp
  35% of sequence in fragments shorter than    5972 bp
  40% of sequence in fragments shorter than    6780 bp
  45% of sequence in fragments shorter than    7638 bp
  50% of sequence in fragments shorter than    8564 bp
  55% of sequence in fragments shorter than    9552 bp
  60% of sequence in fragments shorter than   10656 bp
  65% of sequence in fragments shorter than   11870 bp
  70% of sequence in fragments shorter than   13294 bp
  75% of sequence in fragments shorter than   14931 bp
  80% of sequence in fragments shorter than   16904 bp
  85% of sequence in fragments shorter than   19439 bp
  90% of sequence in fragments shorter than   23127 bp
  95% of sequence in fragments shorter than   29747 bp

# EcoRV.SexAI (GATATC+ACCWGGT) double digest, 743 thousand fragments (446473+297900 sites), 4005 bp average fragment length
   5% of sequence in fragments shorter than    1394 bp
  10% of sequence in fragments shorter than    2123 bp
  15% of sequence in fragments shorter than    2746 bp
  20% of sequence in fragments shorter than    3325 bp
  25% of sequence in fragments shorter than    3895 bp
  30% of sequence in fragments shorter than    4462 bp
  35% of sequence in fragments shorter than    5035 bp
  40% of sequence in fragments shorter than    5630 bp
  45% of sequence in fragments shorter than    6251 bp
  50% of sequence in fragments shorter than    6913 bp
  55% of sequence in fragments shorter than    7617 bp
  60% of sequence in fragments shorter than    8379 bp
  65% of sequence in fragments shorter than    9234 bp
  70% of sequence in fragments shorter than   10203 bp
  75% of sequence in fragments shorter than   11341 bp
  80% of sequence in fragments shorter than   12710 bp
  85% of sequence in fragments shorter than   14458 bp
  90% of sequence in fragments shorter than   16942 bp
  95% of sequence in fragments shorter than   21634 bp

# AclI.EciI (AACGTT+GGCGGA) double digest, 743 thousand fragments (172706+571273 sites), 4007 bp average fragment length
   5% of sequence in fragments shorter than    1315 bp
  10% of sequence in fragments shorter than    2118 bp
  15% of sequence in fragments shorter than    2853 bp
  20% of sequence in fragments shorter than    3549 bp
  25% of sequence in fragments shorter than    4274 bp
  30% of sequence in fragments shorter than    5032 bp
  35% of sequence in fragments shorter than    5815 bp
  40% of sequence in fragments shorter than    6651 bp
  45% of sequence in fragments shorter than    7544 bp
  50% of sequence in fragments shorter than    8507 bp
  55% of sequence in fragments shorter than    9573 bp
  60% of sequence in fragments shorter than   10776 bp
  65% of sequence in fragments shorter than   12121 bp
  70% of sequence in fragments shorter than   13672 bp
  75% of sequence in fragments shorter than   15540 bp
  80% of sequence in fragments shorter than   17848 bp
  85% of sequence in fragments shorter than   20800 bp
  90% of sequence in fragments shorter than   25054 bp
  95% of sequence in fragments shorter than   33038 bp

# FnuDII.NotI (CGCG+GCGGCCGC) double digest, 743 thousand fragments (733940+9795 sites), 4008 bp average fragment length
   5% of sequence in fragments shorter than    1818 bp
  10% of sequence in fragments shorter than    3232 bp
  15% of sequence in fragments shorter than    4598 bp
  20% of sequence in fragments shorter than    5981 bp
  25% of sequence in fragments shorter than    7429 bp
  30% of sequence in fragments shorter than    8974 bp
  35% of sequence in fragments shorter than   10642 bp
  40% of sequence in fragments shorter than   12444 bp
  45% of sequence in fragments shorter than   14471 bp
  50% of sequence in fragments shorter than   16715 bp
  55% of sequence in fragments shorter than   19193 bp
  60% of sequence in fragments shorter than   21980 bp
  65% of sequence in fragments shorter than   25167 bp
  70% of sequence in fragments shorter than   28887 bp
  75% of sequence in fragments shorter than   33362 bp
  80% of sequence in fragments shorter than   39036 bp
  85% of sequence in fragments shorter than   46638 bp
  90% of sequence in fragments shorter than   57655 bp
  95% of sequence in fragments shorter than   79736 bp

# FnuDII.RsrII (CGCG+CGGWCCG) double digest, 743 thousand fragments (733940+9764 sites), 4008 bp average fragment length
   5% of sequence in fragments shorter than    1806 bp
  10% of sequence in fragments shorter than    3212 bp
  15% of sequence in fragments shorter than    4567 bp
  20% of sequence in fragments shorter than    5940 bp
  25% of sequence in fragments shorter than    7376 bp
  30% of sequence in fragments shorter than    8915 bp
  35% of sequence in fragments shorter than   10569 bp
  40% of sequence in fragments shorter than   12363 bp
  45% of sequence in fragments shorter than   14370 bp
  50% of sequence in fragments shorter than   16587 bp
  55% of sequence in fragments shorter than   19061 bp
  60% of sequence in fragments shorter than   21821 bp
  65% of sequence in fragments shorter than   25005 bp
  70% of sequence in fragments shorter than   28694 bp
  75% of sequence in fragments shorter than   33087 bp
  80% of sequence in fragments shorter than   38738 bp
  85% of sequence in fragments shorter than   46282 bp
  90% of sequence in fragments shorter than   57177 bp
  95% of sequence in fragments shorter than   79107 bp

# AscI.BclI (GGCGCGCC+TGATCA) double digest, 743 thousand fragments (4751+738785 sites), 4009 bp average fragment length
   5% of sequence in fragments shorter than    1416 bp
  10% of sequence in fragments shorter than    2118 bp
  15% of sequence in fragments shorter than    2719 bp
  20% of sequence in fragments shorter than    3297 bp
  25% of sequence in fragments shorter than    3863 bp
  30% of sequence in fragments shorter than    4414 bp
  35% of sequence in fragments shorter than    4980 bp
  40% of sequence in fragments shorter than    5559 bp
  45% of sequence in fragments shorter than    6174 bp
  50% of sequence in fragments shorter than    6822 bp
  55% of sequence in fragments shorter than    7519 bp
  60% of sequence in fragments shorter than    8282 bp
  65% of sequence in fragments shorter than    9123 bp
  70% of sequence in fragments shorter than   10105 bp
  75% of sequence in fragments shorter than   11255 bp
  80% of sequence in fragments shorter than   12637 bp
  85% of sequence in fragments shorter than   14466 bp
  90% of sequence in fragments shorter than   17207 bp
  95% of sequence in fragments shorter than   22984 bp

# BamHI.SapI (GGATCC+GCTCTTC) double digest, 742 thousand fragments (365999+377161 sites), 4011 bp average fragment length
   5% of sequence in fragments shorter than    1383 bp
  10% of sequence in fragments shorter than    2106 bp
  15% of sequence in fragments shorter than    2701 bp
  20% of sequence in fragments shorter than    3305 bp
  25% of sequence in fragments shorter than    3892 bp
  30% of sequence in fragments shorter than    4494 bp
  35% of sequence in fragments shorter than    5106 bp
  40% of sequence in fragments shorter than    5744 bp
  45% of sequence in fragments shorter than    6412 bp
  50% of sequence in fragments shorter than    7139 bp
  55% of sequence in fragments shorter than    7910 bp
  60% of sequence in fragments shorter than    8752 bp
  65% of sequence in fragments shorter than    9702 bp
  70% of sequence in fragments shorter than   10773 bp
  75% of sequence in fragments shorter than   12063 bp
  80% of sequence in fragments shorter than   13619 bp
  85% of sequence in fragments shorter than   15616 bp
  90% of sequence in fragments shorter than   18585 bp
  95% of sequence in fragments shorter than   24554 bp

# BamHI.SmaI (GGATCC+CCCGGG) double digest, 742 thousand fragments (365999+376940 sites), 4012 bp average fragment length
   5% of sequence in fragments shorter than    1328 bp
  10% of sequence in fragments shorter than    2089 bp
  15% of sequence in fragments shorter than    2794 bp
  20% of sequence in fragments shorter than    3476 bp
  25% of sequence in fragments shorter than    4160 bp
  30% of sequence in fragments shorter than    4849 bp
  35% of sequence in fragments shorter than    5571 bp
  40% of sequence in fragments shorter than    6339 bp
  45% of sequence in fragments shorter than    7165 bp
  50% of sequence in fragments shorter than    8054 bp
  55% of sequence in fragments shorter than    9036 bp
  60% of sequence in fragments shorter than   10144 bp
  65% of sequence in fragments shorter than   11361 bp
  70% of sequence in fragments shorter than   12783 bp
  75% of sequence in fragments shorter than   14489 bp
  80% of sequence in fragments shorter than   16561 bp
  85% of sequence in fragments shorter than   19333 bp
  90% of sequence in fragments shorter than   23432 bp
  95% of sequence in fragments shorter than   31885 bp

# AscI.FnuDII (GGCGCGCC+CGCG) double digest, 738 thousand fragments (4751+733940 sites), 4036 bp average fragment length
   5% of sequence in fragments shorter than    1828 bp
  10% of sequence in fragments shorter than    3245 bp
  15% of sequence in fragments shorter than    4614 bp
  20% of sequence in fragments shorter than    6000 bp
  25% of sequence in fragments shorter than    7451 bp
  30% of sequence in fragments shorter than    8999 bp
  35% of sequence in fragments shorter than   10672 bp
  40% of sequence in fragments shorter than   12472 bp
  45% of sequence in fragments shorter than   14502 bp
  50% of sequence in fragments shorter than   16747 bp
  55% of sequence in fragments shorter than   19236 bp
  60% of sequence in fragments shorter than   22038 bp
  65% of sequence in fragments shorter than   25222 bp
  70% of sequence in fragments shorter than   28949 bp
  75% of sequence in fragments shorter than   33422 bp
  80% of sequence in fragments shorter than   39112 bp
  85% of sequence in fragments shorter than   46701 bp
  90% of sequence in fragments shorter than   57740 bp
  95% of sequence in fragments shorter than   79981 bp

# BciVI.PmeI (GTATCC+GTTTAAAC) double digest, 737 thousand fragments (696093+42094 sites), 4039 bp average fragment length
   5% of sequence in fragments shorter than    1431 bp
  10% of sequence in fragments shorter than    2117 bp
  15% of sequence in fragments shorter than    2749 bp
  20% of sequence in fragments shorter than    3339 bp
  25% of sequence in fragments shorter than    3913 bp
  30% of sequence in fragments shorter than    4479 bp
  35% of sequence in fragments shorter than    5060 bp
  40% of sequence in fragments shorter than    5658 bp
  45% of sequence in fragments shorter than    6285 bp
  50% of sequence in fragments shorter than    6944 bp
  55% of sequence in fragments shorter than    7657 bp
  60% of sequence in fragments shorter than    8445 bp
  65% of sequence in fragments shorter than    9321 bp
  70% of sequence in fragments shorter than   10314 bp
  75% of sequence in fragments shorter than   11460 bp
  80% of sequence in fragments shorter than   12877 bp
  85% of sequence in fragments shorter than   14707 bp
  90% of sequence in fragments shorter than   17423 bp
  95% of sequence in fragments shorter than   22545 bp

# AclI.MfeI (AACGTT+CAATTG) double digest, 736 thousand fragments (172706+564303 sites), 4045 bp average fragment length
   5% of sequence in fragments shorter than    1383 bp
  10% of sequence in fragments shorter than    2106 bp
  15% of sequence in fragments shorter than    2745 bp
  20% of sequence in fragments shorter than    3328 bp
  25% of sequence in fragments shorter than    3916 bp
  30% of sequence in fragments shorter than    4508 bp
  35% of sequence in fragments shorter than    5107 bp
  40% of sequence in fragments shorter than    5727 bp
  45% of sequence in fragments shorter than    6381 bp
  50% of sequence in fragments shorter than    7082 bp
  55% of sequence in fragments shorter than    7837 bp
  60% of sequence in fragments shorter than    8675 bp
  65% of sequence in fragments shorter than    9614 bp
  70% of sequence in fragments shorter than   10704 bp
  75% of sequence in fragments shorter than   11956 bp
  80% of sequence in fragments shorter than   13539 bp
  85% of sequence in fragments shorter than   15679 bp
  90% of sequence in fragments shorter than   18828 bp
  95% of sequence in fragments shorter than   25208 bp

# BceAI.SalI (ACGGC+GTCGAC) double digest, 736 thousand fragments (705178+31396 sites), 4048 bp average fragment length
   5% of sequence in fragments shorter than    1322 bp
  10% of sequence in fragments shorter than    2175 bp
  15% of sequence in fragments shorter than    2961 bp
  20% of sequence in fragments shorter than    3733 bp
  25% of sequence in fragments shorter than    4504 bp
  30% of sequence in fragments shorter than    5305 bp
  35% of sequence in fragments shorter than    6121 bp
  40% of sequence in fragments shorter than    6983 bp
  45% of sequence in fragments shorter than    7914 bp
  50% of sequence in fragments shorter than    8911 bp
  55% of sequence in fragments shorter than   10007 bp
  60% of sequence in fragments shorter than   11225 bp
  65% of sequence in fragments shorter than   12576 bp
  70% of sequence in fragments shorter than   14150 bp
  75% of sequence in fragments shorter than   15983 bp
  80% of sequence in fragments shorter than   18260 bp
  85% of sequence in fragments shorter than   21203 bp
  90% of sequence in fragments shorter than   25442 bp
  95% of sequence in fragments shorter than   33347 bp

# BstEII.SpeI (GGTNACC+ACTAGT) double digest, 735 thousand fragments (335865+400286 sites), 4050 bp average fragment length
   5% of sequence in fragments shorter than    1435 bp
  10% of sequence in fragments shorter than    2151 bp
  15% of sequence in fragments shorter than    2770 bp
  20% of sequence in fragments shorter than    3359 bp
  25% of sequence in fragments shorter than    3865 bp
  30% of sequence in fragments shorter than    4419 bp
  35% of sequence in fragments shorter than    4994 bp
  40% of sequence in fragments shorter than    5577 bp
  45% of sequence in fragments shorter than    6193 bp
  50% of sequence in fragments shorter than    6842 bp
  55% of sequence in fragments shorter than    7535 bp
  60% of sequence in fragments shorter than    8303 bp
  65% of sequence in fragments shorter than    9165 bp
  70% of sequence in fragments shorter than   10117 bp
  75% of sequence in fragments shorter than   11244 bp
  80% of sequence in fragments shorter than   12608 bp
  85% of sequence in fragments shorter than   14396 bp
  90% of sequence in fragments shorter than   17021 bp
  95% of sequence in fragments shorter than   22064 bp

# SacI.SnaBI (GAGCTC+TACGTA) double digest, 735 thousand fragments (603323+132299 sites), 4053 bp average fragment length
   5% of sequence in fragments shorter than    1393 bp
  10% of sequence in fragments shorter than    2127 bp
  15% of sequence in fragments shorter than    2769 bp
  20% of sequence in fragments shorter than    3385 bp
  25% of sequence in fragments shorter than    3994 bp
  30% of sequence in fragments shorter than    4586 bp
  35% of sequence in fragments shorter than    5194 bp
  40% of sequence in fragments shorter than    5827 bp
  45% of sequence in fragments shorter than    6493 bp
  50% of sequence in fragments shorter than    7195 bp
  55% of sequence in fragments shorter than    7949 bp
  60% of sequence in fragments shorter than    8774 bp
  65% of sequence in fragments shorter than    9696 bp
  70% of sequence in fragments shorter than   10751 bp
  75% of sequence in fragments shorter than   11970 bp
  80% of sequence in fragments shorter than   13473 bp
  85% of sequence in fragments shorter than   15396 bp
  90% of sequence in fragments shorter than   18207 bp
  95% of sequence in fragments shorter than   23562 bp

# BsaAI.MluI (YACGTR+ACGCGT) double digest, 734 thousand fragments (713801+21415 sites), 4055 bp average fragment length
   5% of sequence in fragments shorter than    1419 bp
  10% of sequence in fragments shorter than    2174 bp
  15% of sequence in fragments shorter than    2843 bp
  20% of sequence in fragments shorter than    3460 bp
  25% of sequence in fragments shorter than    4047 bp
  30% of sequence in fragments shorter than    4649 bp
  35% of sequence in fragments shorter than    5275 bp
  40% of sequence in fragments shorter than    5926 bp
  45% of sequence in fragments shorter than    6605 bp
  50% of sequence in fragments shorter than    7317 bp
  55% of sequence in fragments shorter than    8099 bp
  60% of sequence in fragments shorter than    8940 bp
  65% of sequence in fragments shorter than    9881 bp
  70% of sequence in fragments shorter than   10941 bp
  75% of sequence in fragments shorter than   12178 bp
  80% of sequence in fragments shorter than   13701 bp
  85% of sequence in fragments shorter than   15648 bp
  90% of sequence in fragments shorter than   18417 bp
  95% of sequence in fragments shorter than   23388 bp

# EcoRV.KpnI (GATATC+GGTACC) double digest, 734 thousand fragments (446473+287974 sites), 4059 bp average fragment length
   5% of sequence in fragments shorter than    1344 bp
  10% of sequence in fragments shorter than    2085 bp
  15% of sequence in fragments shorter than    2755 bp
  20% of sequence in fragments shorter than    3379 bp
  25% of sequence in fragments shorter than    3996 bp
  30% of sequence in fragments shorter than    4610 bp
  35% of sequence in fragments shorter than    5228 bp
  40% of sequence in fragments shorter than    5866 bp
  45% of sequence in fragments shorter than    6538 bp
  50% of sequence in fragments shorter than    7250 bp
  55% of sequence in fragments shorter than    8018 bp
  60% of sequence in fragments shorter than    8854 bp
  65% of sequence in fragments shorter than    9785 bp
  70% of sequence in fragments shorter than   10844 bp
  75% of sequence in fragments shorter than   12074 bp
  80% of sequence in fragments shorter than   13581 bp
  85% of sequence in fragments shorter than   15542 bp
  90% of sequence in fragments shorter than   18377 bp
  95% of sequence in fragments shorter than   23770 bp

# EcoRV.NheI (GATATC+GCTAGC) double digest, 733 thousand fragments (446473+287384 sites), 4063 bp average fragment length
   5% of sequence in fragments shorter than    1425 bp
  10% of sequence in fragments shorter than    2112 bp
  15% of sequence in fragments shorter than    2755 bp
  20% of sequence in fragments shorter than    3387 bp
  25% of sequence in fragments shorter than    4011 bp
  30% of sequence in fragments shorter than    4639 bp
  35% of sequence in fragments shorter than    5274 bp
  40% of sequence in fragments shorter than    5934 bp
  45% of sequence in fragments shorter than    6624 bp
  50% of sequence in fragments shorter than    7351 bp
  55% of sequence in fragments shorter than    8137 bp
  60% of sequence in fragments shorter than    9000 bp
  65% of sequence in fragments shorter than    9957 bp
  70% of sequence in fragments shorter than   11056 bp
  75% of sequence in fragments shorter than   12310 bp
  80% of sequence in fragments shorter than   13883 bp
  85% of sequence in fragments shorter than   15966 bp
  90% of sequence in fragments shorter than   19028 bp
  95% of sequence in fragments shorter than   24905 bp

# ApaLI.BsrBI (GTGCAC+CCGCTC) double digest, 731 thousand fragments (496255+235523 sites), 4074 bp average fragment length
   5% of sequence in fragments shorter than    1403 bp
  10% of sequence in fragments shorter than    2189 bp
  15% of sequence in fragments shorter than    2894 bp
  20% of sequence in fragments shorter than    3531 bp
  25% of sequence in fragments shorter than    4211 bp
  30% of sequence in fragments shorter than    4894 bp
  35% of sequence in fragments shorter than    5601 bp
  40% of sequence in fragments shorter than    6323 bp
  45% of sequence in fragments shorter than    7098 bp
  50% of sequence in fragments shorter than    7936 bp
  55% of sequence in fragments shorter than    8829 bp
  60% of sequence in fragments shorter than    9808 bp
  65% of sequence in fragments shorter than   10925 bp
  70% of sequence in fragments shorter than   12176 bp
  75% of sequence in fragments shorter than   13678 bp
  80% of sequence in fragments shorter than   15512 bp
  85% of sequence in fragments shorter than   17898 bp
  90% of sequence in fragments shorter than   21319 bp
  95% of sequence in fragments shorter than   27677 bp

# HgaI.PacI (GACGC+TTAATTAA) double digest, 730 thousand fragments (571889+159455 sites), 4076 bp average fragment length
   5% of sequence in fragments shorter than    1413 bp
  10% of sequence in fragments shorter than    2293 bp
  15% of sequence in fragments shorter than    3065 bp
  20% of sequence in fragments shorter than    3810 bp
  25% of sequence in fragments shorter than    4557 bp
  30% of sequence in fragments shorter than    5314 bp
  35% of sequence in fragments shorter than    6082 bp
  40% of sequence in fragments shorter than    6883 bp
  45% of sequence in fragments shorter than    7732 bp
  50% of sequence in fragments shorter than    8640 bp
  55% of sequence in fragments shorter than    9621 bp
  60% of sequence in fragments shorter than   10685 bp
  65% of sequence in fragments shorter than   11885 bp
  70% of sequence in fragments shorter than   13257 bp
  75% of sequence in fragments shorter than   14870 bp
  80% of sequence in fragments shorter than   16826 bp
  85% of sequence in fragments shorter than   19360 bp
  90% of sequence in fragments shorter than   22903 bp
  95% of sequence in fragments shorter than   29772 bp

# EciI.PacI (GGCGGA+TTAATTAA) double digest, 730 thousand fragments (571273+159455 sites), 4079 bp average fragment length
   5% of sequence in fragments shorter than    1381 bp
  10% of sequence in fragments shorter than    2216 bp
  15% of sequence in fragments shorter than    2959 bp
  20% of sequence in fragments shorter than    3693 bp
  25% of sequence in fragments shorter than    4441 bp
  30% of sequence in fragments shorter than    5211 bp
  35% of sequence in fragments shorter than    6017 bp
  40% of sequence in fragments shorter than    6873 bp
  45% of sequence in fragments shorter than    7787 bp
  50% of sequence in fragments shorter than    8771 bp
  55% of sequence in fragments shorter than    9862 bp
  60% of sequence in fragments shorter than   11060 bp
  65% of sequence in fragments shorter than   12433 bp
  70% of sequence in fragments shorter than   14036 bp
  75% of sequence in fragments shorter than   15923 bp
  80% of sequence in fragments shorter than   18252 bp
  85% of sequence in fragments shorter than   21336 bp
  90% of sequence in fragments shorter than   26040 bp
  95% of sequence in fragments shorter than   35688 bp

# AflII.ClaI (CTTAAG+ATCGAT) double digest, 729 thousand fragments (643284+86477 sites), 4085 bp average fragment length
   5% of sequence in fragments shorter than    1436 bp
  10% of sequence in fragments shorter than    2161 bp
  15% of sequence in fragments shorter than    2782 bp
  20% of sequence in fragments shorter than    3372 bp
  25% of sequence in fragments shorter than    3937 bp
  30% of sequence in fragments shorter than    4511 bp
  35% of sequence in fragments shorter than    5086 bp
  40% of sequence in fragments shorter than    5686 bp
  45% of sequence in fragments shorter than    6319 bp
  50% of sequence in fragments shorter than    6994 bp
  55% of sequence in fragments shorter than    7717 bp
  60% of sequence in fragments shorter than    8510 bp
  65% of sequence in fragments shorter than    9401 bp
  70% of sequence in fragments shorter than   10423 bp
  75% of sequence in fragments shorter than   11619 bp
  80% of sequence in fragments shorter than   13102 bp
  85% of sequence in fragments shorter than   15084 bp
  90% of sequence in fragments shorter than   18036 bp
  95% of sequence in fragments shorter than   24717 bp

# HgaI.Hpy99I (GACGC+CGWCG) double digest, 729 thousand fragments (571889+157917 sites), 4085 bp average fragment length
   5% of sequence in fragments shorter than    1461 bp
  10% of sequence in fragments shorter than    2496 bp
  15% of sequence in fragments shorter than    3425 bp
  20% of sequence in fragments shorter than    4372 bp
  25% of sequence in fragments shorter than    5339 bp
  30% of sequence in fragments shorter than    6319 bp
  35% of sequence in fragments shorter than    7336 bp
  40% of sequence in fragments shorter than    8428 bp
  45% of sequence in fragments shorter than    9597 bp
  50% of sequence in fragments shorter than   10868 bp
  55% of sequence in fragments shorter than   12264 bp
  60% of sequence in fragments shorter than   13834 bp
  65% of sequence in fragments shorter than   15595 bp
  70% of sequence in fragments shorter than   17567 bp
  75% of sequence in fragments shorter than   19957 bp
  80% of sequence in fragments shorter than   22806 bp
  85% of sequence in fragments shorter than   26675 bp
  90% of sequence in fragments shorter than   32373 bp
  95% of sequence in fragments shorter than   42990 bp

# EciI.Hpy99I (GGCGGA+CGWCG) double digest, 728 thousand fragments (571273+157917 sites), 4088 bp average fragment length
   5% of sequence in fragments shorter than    1332 bp
  10% of sequence in fragments shorter than    2235 bp
  15% of sequence in fragments shorter than    3067 bp
  20% of sequence in fragments shorter than    3924 bp
  25% of sequence in fragments shorter than    4822 bp
  30% of sequence in fragments shorter than    5780 bp
  35% of sequence in fragments shorter than    6795 bp
  40% of sequence in fragments shorter than    7890 bp
  45% of sequence in fragments shorter than    9092 bp
  50% of sequence in fragments shorter than   10468 bp
  55% of sequence in fragments shorter than   11955 bp
  60% of sequence in fragments shorter than   13667 bp
  65% of sequence in fragments shorter than   15648 bp
  70% of sequence in fragments shorter than   17971 bp
  75% of sequence in fragments shorter than   20728 bp
  80% of sequence in fragments shorter than   24138 bp
  85% of sequence in fragments shorter than   28668 bp
  90% of sequence in fragments shorter than   35492 bp
  95% of sequence in fragments shorter than   48910 bp

# BsaAI.SgrAI (YACGTR+CRCCGGYG) double digest, 728 thousand fragments (713801+15270 sites), 4089 bp average fragment length
   5% of sequence in fragments shorter than    1431 bp
  10% of sequence in fragments shorter than    2191 bp
  15% of sequence in fragments shorter than    2864 bp
  20% of sequence in fragments shorter than    3482 bp
  25% of sequence in fragments shorter than    4081 bp
  30% of sequence in fragments shorter than    4687 bp
  35% of sequence in fragments shorter than    5318 bp
  40% of sequence in fragments shorter than    5972 bp
  45% of sequence in fragments shorter than    6656 bp
  50% of sequence in fragments shorter than    7374 bp
  55% of sequence in fragments shorter than    8164 bp
  60% of sequence in fragments shorter than    9004 bp
  65% of sequence in fragments shorter than    9952 bp
  70% of sequence in fragments shorter than   11013 bp
  75% of sequence in fragments shorter than   12267 bp
  80% of sequence in fragments shorter than   13790 bp
  85% of sequence in fragments shorter than   15749 bp
  90% of sequence in fragments shorter than   18539 bp
  95% of sequence in fragments shorter than   23547 bp

# NaeI.SacI (GCCGGC+GAGCTC) double digest, 728 thousand fragments (125692+603323 sites), 4089 bp average fragment length
   5% of sequence in fragments shorter than    1373 bp
  10% of sequence in fragments shorter than    2158 bp
  15% of sequence in fragments shorter than    2870 bp
  20% of sequence in fragments shorter than    3557 bp
  25% of sequence in fragments shorter than    4241 bp
  30% of sequence in fragments shorter than    4921 bp
  35% of sequence in fragments shorter than    5633 bp
  40% of sequence in fragments shorter than    6381 bp
  45% of sequence in fragments shorter than    7164 bp
  50% of sequence in fragments shorter than    8006 bp
  55% of sequence in fragments shorter than    8924 bp
  60% of sequence in fragments shorter than    9954 bp
  65% of sequence in fragments shorter than   11093 bp
  70% of sequence in fragments shorter than   12381 bp
  75% of sequence in fragments shorter than   13921 bp
  80% of sequence in fragments shorter than   15805 bp
  85% of sequence in fragments shorter than   18292 bp
  90% of sequence in fragments shorter than   22048 bp
  95% of sequence in fragments shorter than   29404 bp

# BsaAI.NruI (YACGTR+TCGCGA) double digest, 728 thousand fragments (713801+14872 sites), 4092 bp average fragment length
   5% of sequence in fragments shorter than    1433 bp
  10% of sequence in fragments shorter than    2191 bp
  15% of sequence in fragments shorter than    2863 bp
  20% of sequence in fragments shorter than    3478 bp
  25% of sequence in fragments shorter than    4073 bp
  30% of sequence in fragments shorter than    4677 bp
  35% of sequence in fragments shorter than    5308 bp
  40% of sequence in fragments shorter than    5959 bp
  45% of sequence in fragments shorter than    6642 bp
  50% of sequence in fragments shorter than    7358 bp
  55% of sequence in fragments shorter than    8144 bp
  60% of sequence in fragments shorter than    8982 bp
  65% of sequence in fragments shorter than    9927 bp
  70% of sequence in fragments shorter than   10987 bp
  75% of sequence in fragments shorter than   12228 bp
  80% of sequence in fragments shorter than   13747 bp
  85% of sequence in fragments shorter than   15704 bp
  90% of sequence in fragments shorter than   18458 bp
  95% of sequence in fragments shorter than   23413 bp

# BsaAI.PvuI (YACGTR+CGATCG) double digest, 727 thousand fragments (713801+14186 sites), 4095 bp average fragment length
   5% of sequence in fragments shorter than    1436 bp
  10% of sequence in fragments shorter than    2187 bp
  15% of sequence in fragments shorter than    2861 bp
  20% of sequence in fragments shorter than    3478 bp
  25% of sequence in fragments shorter than    4074 bp
  30% of sequence in fragments shorter than    4680 bp
  35% of sequence in fragments shorter than    5310 bp
  40% of sequence in fragments shorter than    5962 bp
  45% of sequence in fragments shorter than    6645 bp
  50% of sequence in fragments shorter than    7365 bp
  55% of sequence in fragments shorter than    8154 bp
  60% of sequence in fragments shorter than    8994 bp
  65% of sequence in fragments shorter than    9941 bp
  70% of sequence in fragments shorter than   11001 bp
  75% of sequence in fragments shorter than   12247 bp
  80% of sequence in fragments shorter than   13762 bp
  85% of sequence in fragments shorter than   15711 bp
  90% of sequence in fragments shorter than   18456 bp
  95% of sequence in fragments shorter than   23373 bp

# BciVI.SalI (GTATCC+GTCGAC) double digest, 727 thousand fragments (696093+31396 sites), 4098 bp average fragment length
   5% of sequence in fragments shorter than    1454 bp
  10% of sequence in fragments shorter than    2151 bp
  15% of sequence in fragments shorter than    2793 bp
  20% of sequence in fragments shorter than    3395 bp
  25% of sequence in fragments shorter than    3972 bp
  30% of sequence in fragments shorter than    4548 bp
  35% of sequence in fragments shorter than    5140 bp
  40% of sequence in fragments shorter than    5750 bp
  45% of sequence in fragments shorter than    6384 bp
  50% of sequence in fragments shorter than    7055 bp
  55% of sequence in fragments shorter than    7781 bp
  60% of sequence in fragments shorter than    8573 bp
  65% of sequence in fragments shorter than    9452 bp
  70% of sequence in fragments shorter than   10454 bp
  75% of sequence in fragments shorter than   11619 bp
  80% of sequence in fragments shorter than   13034 bp
  85% of sequence in fragments shorter than   14874 bp
  90% of sequence in fragments shorter than   17556 bp
  95% of sequence in fragments shorter than   22573 bp

# BsaAI.FseI (YACGTR+GGCCGGCC) double digest, 727 thousand fragments (713801+13662 sites), 4098 bp average fragment length
   5% of sequence in fragments shorter than    1434 bp
  10% of sequence in fragments shorter than    2194 bp
  15% of sequence in fragments shorter than    2868 bp
  20% of sequence in fragments shorter than    3484 bp
  25% of sequence in fragments shorter than    4085 bp
  30% of sequence in fragments shorter than    4692 bp
  35% of sequence in fragments shorter than    5327 bp
  40% of sequence in fragments shorter than    5980 bp
  45% of sequence in fragments shorter than    6666 bp
  50% of sequence in fragments shorter than    7387 bp
  55% of sequence in fragments shorter than    8179 bp
  60% of sequence in fragments shorter than    9022 bp
  65% of sequence in fragments shorter than    9972 bp
  70% of sequence in fragments shorter than   11040 bp
  75% of sequence in fragments shorter than   12293 bp
  80% of sequence in fragments shorter than   13823 bp
  85% of sequence in fragments shorter than   15794 bp
  90% of sequence in fragments shorter than   18589 bp
  95% of sequence in fragments shorter than   23623 bp

# AvrII.SnaBI (CCTAGG+TACGTA) double digest, 726 thousand fragments (594956+132299 sites), 4099 bp average fragment length
   5% of sequence in fragments shorter than    1428 bp
  10% of sequence in fragments shorter than    2195 bp
  15% of sequence in fragments shorter than    2842 bp
  20% of sequence in fragments shorter than    3452 bp
  25% of sequence in fragments shorter than    4041 bp
  30% of sequence in fragments shorter than    4626 bp
  35% of sequence in fragments shorter than    5217 bp
  40% of sequence in fragments shorter than    5825 bp
  45% of sequence in fragments shorter than    6466 bp
  50% of sequence in fragments shorter than    7135 bp
  55% of sequence in fragments shorter than    7858 bp
  60% of sequence in fragments shorter than    8630 bp
  65% of sequence in fragments shorter than    9517 bp
  70% of sequence in fragments shorter than   10504 bp
  75% of sequence in fragments shorter than   11658 bp
  80% of sequence in fragments shorter than   13080 bp
  85% of sequence in fragments shorter than   14876 bp
  90% of sequence in fragments shorter than   17508 bp
  95% of sequence in fragments shorter than   22764 bp

# ApaLI.KasI (GTGCAC+GGCGCC) double digest, 726 thousand fragments (496255+230381 sites), 4103 bp average fragment length
   5% of sequence in fragments shorter than    1406 bp
  10% of sequence in fragments shorter than    2181 bp
  15% of sequence in fragments shorter than    2873 bp
  20% of sequence in fragments shorter than    3529 bp
  25% of sequence in fragments shorter than    4187 bp
  30% of sequence in fragments shorter than    4849 bp
  35% of sequence in fragments shorter than    5531 bp
  40% of sequence in fragments shorter than    6169 bp
  45% of sequence in fragments shorter than    6924 bp
  50% of sequence in fragments shorter than    7742 bp
  55% of sequence in fragments shorter than    8619 bp
  60% of sequence in fragments shorter than    9584 bp
  65% of sequence in fragments shorter than   10663 bp
  70% of sequence in fragments shorter than   11905 bp
  75% of sequence in fragments shorter than   13416 bp
  80% of sequence in fragments shorter than   15242 bp
  85% of sequence in fragments shorter than   17642 bp
  90% of sequence in fragments shorter than   21188 bp
  95% of sequence in fragments shorter than   28131 bp

# BceAI.MluI (ACGGC+ACGCGT) double digest, 726 thousand fragments (705178+21415 sites), 4103 bp average fragment length
   5% of sequence in fragments shorter than    1337 bp
  10% of sequence in fragments shorter than    2207 bp
  15% of sequence in fragments shorter than    3014 bp
  20% of sequence in fragments shorter than    3806 bp
  25% of sequence in fragments shorter than    4604 bp
  30% of sequence in fragments shorter than    5424 bp
  35% of sequence in fragments shorter than    6264 bp
  40% of sequence in fragments shorter than    7166 bp
  45% of sequence in fragments shorter than    8127 bp
  50% of sequence in fragments shorter than    9154 bp
  55% of sequence in fragments shorter than   10282 bp
  60% of sequence in fragments shorter than   11542 bp
  65% of sequence in fragments shorter than   12939 bp
  70% of sequence in fragments shorter than   14585 bp
  75% of sequence in fragments shorter than   16488 bp
  80% of sequence in fragments shorter than   18834 bp
  85% of sequence in fragments shorter than   21941 bp
  90% of sequence in fragments shorter than   26283 bp
  95% of sequence in fragments shorter than   34622 bp

# BstEII.HpaI (GGTNACC+GTTAAC) double digest, 724 thousand fragments (335865+389418 sites), 4110 bp average fragment length
   5% of sequence in fragments shorter than    1441 bp
  10% of sequence in fragments shorter than    2184 bp
  15% of sequence in fragments shorter than    2831 bp
  20% of sequence in fragments shorter than    3430 bp
  25% of sequence in fragments shorter than    4008 bp
  30% of sequence in fragments shorter than    4583 bp
  35% of sequence in fragments shorter than    5164 bp
  40% of sequence in fragments shorter than    5765 bp
  45% of sequence in fragments shorter than    6396 bp
  50% of sequence in fragments shorter than    7052 bp
  55% of sequence in fragments shorter than    7777 bp
  60% of sequence in fragments shorter than    8551 bp
  65% of sequence in fragments shorter than    9415 bp
  70% of sequence in fragments shorter than   10405 bp
  75% of sequence in fragments shorter than   11554 bp
  80% of sequence in fragments shorter than   12968 bp
  85% of sequence in fragments shorter than   14781 bp
  90% of sequence in fragments shorter than   17499 bp
  95% of sequence in fragments shorter than   23007 bp

# SacI.XhoI (GAGCTC+CTCGAG) double digest, 724 thousand fragments (603323+121308 sites), 4114 bp average fragment length
   5% of sequence in fragments shorter than    1402 bp
  10% of sequence in fragments shorter than    2168 bp
  15% of sequence in fragments shorter than    2856 bp
  20% of sequence in fragments shorter than    3516 bp
  25% of sequence in fragments shorter than    4173 bp
  30% of sequence in fragments shorter than    4820 bp
  35% of sequence in fragments shorter than    5493 bp
  40% of sequence in fragments shorter than    6198 bp
  45% of sequence in fragments shorter than    6935 bp
  50% of sequence in fragments shorter than    7723 bp
  55% of sequence in fragments shorter than    8585 bp
  60% of sequence in fragments shorter than    9536 bp
  65% of sequence in fragments shorter than   10612 bp
  70% of sequence in fragments shorter than   11831 bp
  75% of sequence in fragments shorter than   13270 bp
  80% of sequence in fragments shorter than   15048 bp
  85% of sequence in fragments shorter than   17372 bp
  90% of sequence in fragments shorter than   20844 bp
  95% of sequence in fragments shorter than   27606 bp

# MfeI.PacI (CAATTG+TTAATTAA) double digest, 723 thousand fragments (564303+159455 sites), 4119 bp average fragment length
   5% of sequence in fragments shorter than    1432 bp
  10% of sequence in fragments shorter than    2173 bp
  15% of sequence in fragments shorter than    2820 bp
  20% of sequence in fragments shorter than    3435 bp
  25% of sequence in fragments shorter than    4045 bp
  30% of sequence in fragments shorter than    4646 bp
  35% of sequence in fragments shorter than    5260 bp
  40% of sequence in fragments shorter than    5902 bp
  45% of sequence in fragments shorter than    6583 bp
  50% of sequence in fragments shorter than    7319 bp
  55% of sequence in fragments shorter than    8111 bp
  60% of sequence in fragments shorter than    9004 bp
  65% of sequence in fragments shorter than   10015 bp
  70% of sequence in fragments shorter than   11191 bp
  75% of sequence in fragments shorter than   12578 bp
  80% of sequence in fragments shorter than   14369 bp
  85% of sequence in fragments shorter than   16803 bp
  90% of sequence in fragments shorter than   20679 bp
  95% of sequence in fragments shorter than   29205 bp

# AclI.SphI (AACGTT+GCATGC) double digest, 723 thousand fragments (172706+550952 sites), 4120 bp average fragment length
   5% of sequence in fragments shorter than    1434 bp
  10% of sequence in fragments shorter than    2165 bp
  15% of sequence in fragments shorter than    2811 bp
  20% of sequence in fragments shorter than    3413 bp
  25% of sequence in fragments shorter than    3990 bp
  30% of sequence in fragments shorter than    4583 bp
  35% of sequence in fragments shorter than    5184 bp
  40% of sequence in fragments shorter than    5799 bp
  45% of sequence in fragments shorter than    6445 bp
  50% of sequence in fragments shorter than    7118 bp
  55% of sequence in fragments shorter than    7853 bp
  60% of sequence in fragments shorter than    8651 bp
  65% of sequence in fragments shorter than    9542 bp
  70% of sequence in fragments shorter than   10557 bp
  75% of sequence in fragments shorter than   11748 bp
  80% of sequence in fragments shorter than   13186 bp
  85% of sequence in fragments shorter than   15026 bp
  90% of sequence in fragments shorter than   17665 bp
  95% of sequence in fragments shorter than   22496 bp

# BsaAI.NotI (YACGTR+GCGGCCGC) double digest, 723 thousand fragments (713801+9795 sites), 4120 bp average fragment length
   5% of sequence in fragments shorter than    1443 bp
  10% of sequence in fragments shorter than    2210 bp
  15% of sequence in fragments shorter than    2889 bp
  20% of sequence in fragments shorter than    3493 bp
  25% of sequence in fragments shorter than    4112 bp
  30% of sequence in fragments shorter than    4722 bp
  35% of sequence in fragments shorter than    5361 bp
  40% of sequence in fragments shorter than    6018 bp
  45% of sequence in fragments shorter than    6705 bp
  50% of sequence in fragments shorter than    7429 bp
  55% of sequence in fragments shorter than    8220 bp
  60% of sequence in fragments shorter than    9074 bp
  65% of sequence in fragments shorter than   10026 bp
  70% of sequence in fragments shorter than   11099 bp
  75% of sequence in fragments shorter than   12355 bp
  80% of sequence in fragments shorter than   13900 bp
  85% of sequence in fragments shorter than   15871 bp
  90% of sequence in fragments shorter than   18671 bp
  95% of sequence in fragments shorter than   23715 bp

# BsaAI.RsrII (YACGTR+CGGWCCG) double digest, 723 thousand fragments (713801+9764 sites), 4120 bp average fragment length
   5% of sequence in fragments shorter than    1443 bp
  10% of sequence in fragments shorter than    2207 bp
  15% of sequence in fragments shorter than    2885 bp
  20% of sequence in fragments shorter than    3492 bp
  25% of sequence in fragments shorter than    4107 bp
  30% of sequence in fragments shorter than    4715 bp
  35% of sequence in fragments shorter than    5352 bp
  40% of sequence in fragments shorter than    6009 bp
  45% of sequence in fragments shorter than    6696 bp
  50% of sequence in fragments shorter than    7420 bp
  55% of sequence in fragments shorter than    8214 bp
  60% of sequence in fragments shorter than    9065 bp
  65% of sequence in fragments shorter than   10017 bp
  70% of sequence in fragments shorter than   11086 bp
  75% of sequence in fragments shorter than   12337 bp
  80% of sequence in fragments shorter than   13875 bp
  85% of sequence in fragments shorter than   15841 bp
  90% of sequence in fragments shorter than   18638 bp
  95% of sequence in fragments shorter than   23651 bp

# ApaLI.SwaI (GTGCAC+ATTTAAAT) double digest, 722 thousand fragments (496255+226602 sites), 4124 bp average fragment length
   5% of sequence in fragments shorter than    1472 bp
  10% of sequence in fragments shorter than    2218 bp
  15% of sequence in fragments shorter than    2865 bp
  20% of sequence in fragments shorter than    3466 bp
  25% of sequence in fragments shorter than    4057 bp
  30% of sequence in fragments shorter than    4647 bp
  35% of sequence in fragments shorter than    5243 bp
  40% of sequence in fragments shorter than    5860 bp
  45% of sequence in fragments shorter than    6476 bp
  50% of sequence in fragments shorter than    7140 bp
  55% of sequence in fragments shorter than    7864 bp
  60% of sequence in fragments shorter than    8647 bp
  65% of sequence in fragments shorter than    9505 bp
  70% of sequence in fragments shorter than   10485 bp
  75% of sequence in fragments shorter than   11639 bp
  80% of sequence in fragments shorter than   13028 bp
  85% of sequence in fragments shorter than   14828 bp
  90% of sequence in fragments shorter than   17502 bp
  95% of sequence in fragments shorter than   22712 bp

# Hpy99I.MfeI (CGWCG+CAATTG) double digest, 721 thousand fragments (157917+564303 sites), 4128 bp average fragment length
   5% of sequence in fragments shorter than    1459 bp
  10% of sequence in fragments shorter than    2229 bp
  15% of sequence in fragments shorter than    2902 bp
  20% of sequence in fragments shorter than    3525 bp
  25% of sequence in fragments shorter than    4130 bp
  30% of sequence in fragments shorter than    4705 bp
  35% of sequence in fragments shorter than    5323 bp
  40% of sequence in fragments shorter than    5957 bp
  45% of sequence in fragments shorter than    6615 bp
  50% of sequence in fragments shorter than    7316 bp
  55% of sequence in fragments shorter than    8074 bp
  60% of sequence in fragments shorter than    8898 bp
  65% of sequence in fragments shorter than    9818 bp
  70% of sequence in fragments shorter than   10843 bp
  75% of sequence in fragments shorter than   12040 bp
  80% of sequence in fragments shorter than   13485 bp
  85% of sequence in fragments shorter than   15347 bp
  90% of sequence in fragments shorter than   18079 bp
  95% of sequence in fragments shorter than   23169 bp

# AvrII.NaeI (CCTAGG+GCCGGC) double digest, 720 thousand fragments (594956+125692 sites), 4137 bp average fragment length
   5% of sequence in fragments shorter than    1420 bp
  10% of sequence in fragments shorter than    2228 bp
  15% of sequence in fragments shorter than    2928 bp
  20% of sequence in fragments shorter than    3585 bp
  25% of sequence in fragments shorter than    4223 bp
  30% of sequence in fragments shorter than    4858 bp
  35% of sequence in fragments shorter than    5510 bp
  40% of sequence in fragments shorter than    6191 bp
  45% of sequence in fragments shorter than    6903 bp
  50% of sequence in fragments shorter than    7664 bp
  55% of sequence in fragments shorter than    8471 bp
  60% of sequence in fragments shorter than    9383 bp
  65% of sequence in fragments shorter than   10386 bp
  70% of sequence in fragments shorter than   11546 bp
  75% of sequence in fragments shorter than   12938 bp
  80% of sequence in fragments shorter than   14625 bp
  85% of sequence in fragments shorter than   16819 bp
  90% of sequence in fragments shorter than   20182 bp
  95% of sequence in fragments shorter than   27209 bp

# BceAI.SgrAI (ACGGC+CRCCGGYG) double digest, 720 thousand fragments (705178+15270 sites), 4138 bp average fragment length
   5% of sequence in fragments shorter than    1354 bp
  10% of sequence in fragments shorter than    2239 bp
  15% of sequence in fragments shorter than    3055 bp
  20% of sequence in fragments shorter than    3854 bp
  25% of sequence in fragments shorter than    4663 bp
  30% of sequence in fragments shorter than    5495 bp
  35% of sequence in fragments shorter than    6348 bp
  40% of sequence in fragments shorter than    7263 bp
  45% of sequence in fragments shorter than    8243 bp
  50% of sequence in fragments shorter than    9279 bp
  55% of sequence in fragments shorter than   10424 bp
  60% of sequence in fragments shorter than   11704 bp
  65% of sequence in fragments shorter than   13126 bp
  70% of sequence in fragments shorter than   14779 bp
  75% of sequence in fragments shorter than   16712 bp
  80% of sequence in fragments shorter than   19091 bp
  85% of sequence in fragments shorter than   22221 bp
  90% of sequence in fragments shorter than   26663 bp
  95% of sequence in fragments shorter than   35126 bp

# BceAI.NruI (ACGGC+TCGCGA) double digest, 719 thousand fragments (705178+14872 sites), 4141 bp average fragment length
   5% of sequence in fragments shorter than    1352 bp
  10% of sequence in fragments shorter than    2229 bp
  15% of sequence in fragments shorter than    3041 bp
  20% of sequence in fragments shorter than    3834 bp
  25% of sequence in fragments shorter than    4637 bp
  30% of sequence in fragments shorter than    5462 bp
  35% of sequence in fragments shorter than    6309 bp
  40% of sequence in fragments shorter than    7214 bp
  45% of sequence in fragments shorter than    8178 bp
  50% of sequence in fragments shorter than    9210 bp
  55% of sequence in fragments shorter than   10342 bp
  60% of sequence in fragments shorter than   11617 bp
  65% of sequence in fragments shorter than   13023 bp
  70% of sequence in fragments shorter than   14671 bp
  75% of sequence in fragments shorter than   16573 bp
  80% of sequence in fragments shorter than   18925 bp
  85% of sequence in fragments shorter than   22033 bp
  90% of sequence in fragments shorter than   26463 bp
  95% of sequence in fragments shorter than   34970 bp

# BceAI.PvuI (ACGGC+CGATCG) double digest, 718 thousand fragments (705178+14186 sites), 4145 bp average fragment length
   5% of sequence in fragments shorter than    1354 bp
  10% of sequence in fragments shorter than    2220 bp
  15% of sequence in fragments shorter than    3033 bp
  20% of sequence in fragments shorter than    3828 bp
  25% of sequence in fragments shorter than    4631 bp
  30% of sequence in fragments shorter than    5457 bp
  35% of sequence in fragments shorter than    6304 bp
  40% of sequence in fragments shorter than    7210 bp
  45% of sequence in fragments shorter than    8174 bp
  50% of sequence in fragments shorter than    9206 bp
  55% of sequence in fragments shorter than   10338 bp
  60% of sequence in fragments shorter than   11613 bp
  65% of sequence in fragments shorter than   13024 bp
  70% of sequence in fragments shorter than   14669 bp
  75% of sequence in fragments shorter than   16583 bp
  80% of sequence in fragments shorter than   18934 bp
  85% of sequence in fragments shorter than   22036 bp
  90% of sequence in fragments shorter than   26415 bp
  95% of sequence in fragments shorter than   34709 bp

# BceAI.FseI (ACGGC+GGCCGGCC) double digest, 718 thousand fragments (705178+13662 sites), 4148 bp average fragment length
   5% of sequence in fragments shorter than    1355 bp
  10% of sequence in fragments shorter than    2239 bp
  15% of sequence in fragments shorter than    3058 bp
  20% of sequence in fragments shorter than    3860 bp
  25% of sequence in fragments shorter than    4672 bp
  30% of sequence in fragments shorter than    5506 bp
  35% of sequence in fragments shorter than    6361 bp
  40% of sequence in fragments shorter than    7280 bp
  45% of sequence in fragments shorter than    8258 bp
  50% of sequence in fragments shorter than    9303 bp
  55% of sequence in fragments shorter than   10450 bp
  60% of sequence in fragments shorter than   11733 bp
  65% of sequence in fragments shorter than   13154 bp
  70% of sequence in fragments shorter than   14812 bp
  75% of sequence in fragments shorter than   16755 bp
  80% of sequence in fragments shorter than   19151 bp
  85% of sequence in fragments shorter than   22287 bp
  90% of sequence in fragments shorter than   26752 bp
  95% of sequence in fragments shorter than   35238 bp

# AscI.BsaAI (GGCGCGCC+YACGTR) double digest, 718 thousand fragments (4751+713801 sites), 4149 bp average fragment length
   5% of sequence in fragments shorter than    1454 bp
  10% of sequence in fragments shorter than    2224 bp
  15% of sequence in fragments shorter than    2907 bp
  20% of sequence in fragments shorter than    3509 bp
  25% of sequence in fragments shorter than    4134 bp
  30% of sequence in fragments shorter than    4746 bp
  35% of sequence in fragments shorter than    5386 bp
  40% of sequence in fragments shorter than    6047 bp
  45% of sequence in fragments shorter than    6738 bp
  50% of sequence in fragments shorter than    7464 bp
  55% of sequence in fragments shorter than    8259 bp
  60% of sequence in fragments shorter than    9111 bp
  65% of sequence in fragments shorter than   10066 bp
  70% of sequence in fragments shorter than   11138 bp
  75% of sequence in fragments shorter than   12398 bp
  80% of sequence in fragments shorter than   13939 bp
  85% of sequence in fragments shorter than   15921 bp
  90% of sequence in fragments shorter than   18719 bp
  95% of sequence in fragments shorter than   23781 bp

Average fragment lengths:   1-150 | 151-250 | 251-450 | 451-750 | 751-1150 | 1151-1650 | 1651-2250 | 2251-3150 | 3151-4150 | 4151+

# BciVI.MluI (GTATCC+ACGCGT) double digest, 717 thousand fragments (696093+21415 sites), 4155 bp average fragment length
   5% of sequence in fragments shorter than    1477 bp
  10% of sequence in fragments shorter than    2183 bp
  15% of sequence in fragments shorter than    2835 bp
  20% of sequence in fragments shorter than    3446 bp
  25% of sequence in fragments shorter than    4032 bp
  30% of sequence in fragments shorter than    4618 bp
  35% of sequence in fragments shorter than    5213 bp
  40% of sequence in fragments shorter than    5831 bp
  45% of sequence in fragments shorter than    6478 bp
  50% of sequence in fragments shorter than    7155 bp
  55% of sequence in fragments shorter than    7892 bp
  60% of sequence in fragments shorter than    8692 bp
  65% of sequence in fragments shorter than    9582 bp
  70% of sequence in fragments shorter than   10593 bp
  75% of sequence in fragments shorter than   11763 bp
  80% of sequence in fragments shorter than   13219 bp
  85% of sequence in fragments shorter than   15066 bp
  90% of sequence in fragments shorter than   17769 bp
  95% of sequence in fragments shorter than   22824 bp

# ClaI.HaeII (ATCGAT+RGCGCY) double digest, 716 thousand fragments (86477+630101 sites), 4161 bp average fragment length
   5% of sequence in fragments shorter than    1278 bp
  10% of sequence in fragments shorter than    2295 bp
  15% of sequence in fragments shorter than    3267 bp
  20% of sequence in fragments shorter than    4191 bp
  25% of sequence in fragments shorter than    5133 bp
  30% of sequence in fragments shorter than    6083 bp
  35% of sequence in fragments shorter than    7077 bp
  40% of sequence in fragments shorter than    8129 bp
  45% of sequence in fragments shorter than    9260 bp
  50% of sequence in fragments shorter than   10475 bp
  55% of sequence in fragments shorter than   11790 bp
  60% of sequence in fragments shorter than   13260 bp
  65% of sequence in fragments shorter than   14931 bp
  70% of sequence in fragments shorter than   16848 bp
  75% of sequence in fragments shorter than   19073 bp
  80% of sequence in fragments shorter than   21793 bp
  85% of sequence in fragments shorter than   25225 bp
  90% of sequence in fragments shorter than   30163 bp
  95% of sequence in fragments shorter than   38585 bp

# AclI.ScaI (AACGTT+AGTACT) double digest, 716 thousand fragments (172706+543793 sites), 4161 bp average fragment length
   5% of sequence in fragments shorter than    1436 bp
  10% of sequence in fragments shorter than    2165 bp
  15% of sequence in fragments shorter than    2813 bp
  20% of sequence in fragments shorter than    3421 bp
  25% of sequence in fragments shorter than    4009 bp
  30% of sequence in fragments shorter than    4616 bp
  35% of sequence in fragments shorter than    5234 bp
  40% of sequence in fragments shorter than    5872 bp
  45% of sequence in fragments shorter than    6542 bp
  50% of sequence in fragments shorter than    7253 bp
  55% of sequence in fragments shorter than    8021 bp
  60% of sequence in fragments shorter than    8868 bp
  65% of sequence in fragments shorter than    9813 bp
  70% of sequence in fragments shorter than   10896 bp
  75% of sequence in fragments shorter than   12179 bp
  80% of sequence in fragments shorter than   13769 bp
  85% of sequence in fragments shorter than   15806 bp
  90% of sequence in fragments shorter than   18837 bp
  95% of sequence in fragments shorter than   24843 bp

# AvrII.XhoI (CCTAGG+CTCGAG) double digest, 715 thousand fragments (594956+121308 sites), 4162 bp average fragment length
   5% of sequence in fragments shorter than    1443 bp
  10% of sequence in fragments shorter than    2223 bp
  15% of sequence in fragments shorter than    2843 bp
  20% of sequence in fragments shorter than    3459 bp
  25% of sequence in fragments shorter than    4068 bp
  30% of sequence in fragments shorter than    4677 bp
  35% of sequence in fragments shorter than    5301 bp
  40% of sequence in fragments shorter than    5947 bp
  45% of sequence in fragments shorter than    6628 bp
  50% of sequence in fragments shorter than    7353 bp
  55% of sequence in fragments shorter than    8126 bp
  60% of sequence in fragments shorter than    8976 bp
  65% of sequence in fragments shorter than    9937 bp
  70% of sequence in fragments shorter than   11021 bp
  75% of sequence in fragments shorter than   12307 bp
  80% of sequence in fragments shorter than   13927 bp
  85% of sequence in fragments shorter than   16031 bp
  90% of sequence in fragments shorter than   19099 bp
  95% of sequence in fragments shorter than   25466 bp

# BceAI.NotI (ACGGC+GCGGCCGC) double digest, 714 thousand fragments (705178+9795 sites), 4170 bp average fragment length
   5% of sequence in fragments shorter than    1367 bp
  10% of sequence in fragments shorter than    2257 bp
  15% of sequence in fragments shorter than    3083 bp
  20% of sequence in fragments shorter than    3889 bp
  25% of sequence in fragments shorter than    4705 bp
  30% of sequence in fragments shorter than    5542 bp
  35% of sequence in fragments shorter than    6403 bp
  40% of sequence in fragments shorter than    7326 bp
  45% of sequence in fragments shorter than    8306 bp
  50% of sequence in fragments shorter than    9352 bp
  55% of sequence in fragments shorter than   10503 bp
  60% of sequence in fragments shorter than   11790 bp
  65% of sequence in fragments shorter than   13221 bp
  70% of sequence in fragments shorter than   14884 bp
  75% of sequence in fragments shorter than   16833 bp
  80% of sequence in fragments shorter than   19238 bp
  85% of sequence in fragments shorter than   22384 bp
  90% of sequence in fragments shorter than   26851 bp
  95% of sequence in fragments shorter than   35347 bp

# BceAI.RsrII (ACGGC+CGGWCCG) double digest, 714 thousand fragments (705178+9764 sites), 4170 bp average fragment length
   5% of sequence in fragments shorter than    1363 bp
  10% of sequence in fragments shorter than    2251 bp
  15% of sequence in fragments shorter than    3073 bp
  20% of sequence in fragments shorter than    3877 bp
  25% of sequence in fragments shorter than    4691 bp
  30% of sequence in fragments shorter than    5525 bp
  35% of sequence in fragments shorter than    6383 bp
  40% of sequence in fragments shorter than    7301 bp
  45% of sequence in fragments shorter than    8280 bp
  50% of sequence in fragments shorter than    9324 bp
  55% of sequence in fragments shorter than   10472 bp
  60% of sequence in fragments shorter than   11754 bp
  65% of sequence in fragments shorter than   13178 bp
  70% of sequence in fragments shorter than   14839 bp
  75% of sequence in fragments shorter than   16780 bp
  80% of sequence in fragments shorter than   19191 bp
  85% of sequence in fragments shorter than   22319 bp
  90% of sequence in fragments shorter than   26765 bp
  95% of sequence in fragments shorter than   35238 bp

# AatII.AflII (GACGTC+CTTAAG) double digest, 714 thousand fragments (71089+643284 sites), 4173 bp average fragment length
   5% of sequence in fragments shorter than    1478 bp
  10% of sequence in fragments shorter than    2217 bp
  15% of sequence in fragments shorter than    2852 bp
  20% of sequence in fragments shorter than    3455 bp
  25% of sequence in fragments shorter than    4025 bp
  30% of sequence in fragments shorter than    4602 bp
  35% of sequence in fragments shorter than    5179 bp
  40% of sequence in fragments shorter than    5786 bp
  45% of sequence in fragments shorter than    6418 bp
  50% of sequence in fragments shorter than    7091 bp
  55% of sequence in fragments shorter than    7824 bp
  60% of sequence in fragments shorter than    8606 bp
  65% of sequence in fragments shorter than    9483 bp
  70% of sequence in fragments shorter than   10489 bp
  75% of sequence in fragments shorter than   11649 bp
  80% of sequence in fragments shorter than   13078 bp
  85% of sequence in fragments shorter than   14963 bp
  90% of sequence in fragments shorter than   17720 bp
  95% of sequence in fragments shorter than   23490 bp

# BsaAI.SnaBI (YACGTR+TACGTA) double digest, 713 thousand fragments (713801+132299 sites), 4177 bp average fragment length
   5% of sequence in fragments shorter than    1466 bp
  10% of sequence in fragments shorter than    2238 bp
  15% of sequence in fragments shorter than    2925 bp
  20% of sequence in fragments shorter than    3528 bp
  25% of sequence in fragments shorter than    4155 bp
  30% of sequence in fragments shorter than    4771 bp
  35% of sequence in fragments shorter than    5414 bp
  40% of sequence in fragments shorter than    6077 bp
  45% of sequence in fragments shorter than    6772 bp
  50% of sequence in fragments shorter than    7500 bp
  55% of sequence in fragments shorter than    8296 bp
  60% of sequence in fragments shorter than    9153 bp
  65% of sequence in fragments shorter than   10115 bp
  70% of sequence in fragments shorter than   11192 bp
  75% of sequence in fragments shorter than   12456 bp
  80% of sequence in fragments shorter than   13996 bp
  85% of sequence in fragments shorter than   15985 bp
  90% of sequence in fragments shorter than   18796 bp
  95% of sequence in fragments shorter than   23864 bp

# BstEII.SapI (GGTNACC+GCTCTTC) double digest, 712 thousand fragments (335865+377161 sites), 4181 bp average fragment length
   5% of sequence in fragments shorter than    1432 bp
  10% of sequence in fragments shorter than    2214 bp
  15% of sequence in fragments shorter than    2889 bp
  20% of sequence in fragments shorter than    3538 bp
  25% of sequence in fragments shorter than    4174 bp
  30% of sequence in fragments shorter than    4813 bp
  35% of sequence in fragments shorter than    5451 bp
  40% of sequence in fragments shorter than    6119 bp
  45% of sequence in fragments shorter than    6828 bp
  50% of sequence in fragments shorter than    7585 bp
  55% of sequence in fragments shorter than    8392 bp
  60% of sequence in fragments shorter than    9285 bp
  65% of sequence in fragments shorter than   10298 bp
  70% of sequence in fragments shorter than   11449 bp
  75% of sequence in fragments shorter than   12793 bp
  80% of sequence in fragments shorter than   14456 bp
  85% of sequence in fragments shorter than   16598 bp
  90% of sequence in fragments shorter than   19848 bp
  95% of sequence in fragments shorter than   26473 bp

# BstEII.SmaI (GGTNACC+CCCGGG) double digest, 712 thousand fragments (335865+376940 sites), 4182 bp average fragment length
   5% of sequence in fragments shorter than    1369 bp
  10% of sequence in fragments shorter than    2183 bp
  15% of sequence in fragments shorter than    2927 bp
  20% of sequence in fragments shorter than    3648 bp
  25% of sequence in fragments shorter than    4379 bp
  30% of sequence in fragments shorter than    5116 bp
  35% of sequence in fragments shorter than    5890 bp
  40% of sequence in fragments shorter than    6696 bp
  45% of sequence in fragments shorter than    7562 bp
  50% of sequence in fragments shorter than    8494 bp
  55% of sequence in fragments shorter than    9509 bp
  60% of sequence in fragments shorter than   10660 bp
  65% of sequence in fragments shorter than   11938 bp
  70% of sequence in fragments shorter than   13436 bp
  75% of sequence in fragments shorter than   15192 bp
  80% of sequence in fragments shorter than   17418 bp
  85% of sequence in fragments shorter than   20351 bp
  90% of sequence in fragments shorter than   24807 bp
  95% of sequence in fragments shorter than   34641 bp

# BciVI.SgrAI (GTATCC+CRCCGGYG) double digest, 710 thousand fragments (696093+15270 sites), 4191 bp average fragment length
   5% of sequence in fragments shorter than    1489 bp
  10% of sequence in fragments shorter than    2201 bp
  15% of sequence in fragments shorter than    2860 bp
  20% of sequence in fragments shorter than    3475 bp
  25% of sequence in fragments shorter than    4067 bp
  30% of sequence in fragments shorter than    4660 bp
  35% of sequence in fragments shorter than    5262 bp
  40% of sequence in fragments shorter than    5884 bp
  45% of sequence in fragments shorter than    6534 bp
  50% of sequence in fragments shorter than    7218 bp
  55% of sequence in fragments shorter than    7962 bp
  60% of sequence in fragments shorter than    8777 bp
  65% of sequence in fragments shorter than    9673 bp
  70% of sequence in fragments shorter than   10690 bp
  75% of sequence in fragments shorter than   11877 bp
  80% of sequence in fragments shorter than   13342 bp
  85% of sequence in fragments shorter than   15221 bp
  90% of sequence in fragments shorter than   17961 bp
  95% of sequence in fragments shorter than   23128 bp

# BciVI.NruI (GTATCC+TCGCGA) double digest, 710 thousand fragments (696093+14872 sites), 4193 bp average fragment length
   5% of sequence in fragments shorter than    1489 bp
  10% of sequence in fragments shorter than    2202 bp
  15% of sequence in fragments shorter than    2859 bp
  20% of sequence in fragments shorter than    3474 bp
  25% of sequence in fragments shorter than    4066 bp
  30% of sequence in fragments shorter than    4659 bp
  35% of sequence in fragments shorter than    5258 bp
  40% of sequence in fragments shorter than    5882 bp
  45% of sequence in fragments shorter than    6532 bp
  50% of sequence in fragments shorter than    7218 bp
  55% of sequence in fragments shorter than    7963 bp
  60% of sequence in fragments shorter than    8777 bp
  65% of sequence in fragments shorter than    9677 bp
  70% of sequence in fragments shorter than   10701 bp
  75% of sequence in fragments shorter than   11881 bp
  80% of sequence in fragments shorter than   13344 bp
  85% of sequence in fragments shorter than   15244 bp
  90% of sequence in fragments shorter than   17997 bp
  95% of sequence in fragments shorter than   23152 bp

# PacI.SphI (TTAATTAA+GCATGC) double digest, 710 thousand fragments (159455+550952 sites), 4196 bp average fragment length
   5% of sequence in fragments shorter than    1495 bp
  10% of sequence in fragments shorter than    2250 bp
  15% of sequence in fragments shorter than    2911 bp
  20% of sequence in fragments shorter than    3535 bp
  25% of sequence in fragments shorter than    4145 bp
  30% of sequence in fragments shorter than    4758 bp
  35% of sequence in fragments shorter than    5378 bp
  40% of sequence in fragments shorter than    6016 bp
  45% of sequence in fragments shorter than    6683 bp
  50% of sequence in fragments shorter than    7390 bp
  55% of sequence in fragments shorter than    8151 bp
  60% of sequence in fragments shorter than    8985 bp
  65% of sequence in fragments shorter than    9913 bp
  70% of sequence in fragments shorter than   10974 bp
  75% of sequence in fragments shorter than   12210 bp
  80% of sequence in fragments shorter than   13714 bp
  85% of sequence in fragments shorter than   15640 bp
  90% of sequence in fragments shorter than   18479 bp
  95% of sequence in fragments shorter than   24068 bp

# BciVI.PvuI (GTATCC+CGATCG) double digest, 709 thousand fragments (696093+14186 sites), 4198 bp average fragment length
   5% of sequence in fragments shorter than    1491 bp
  10% of sequence in fragments shorter than    2197 bp
  15% of sequence in fragments shorter than    2858 bp
  20% of sequence in fragments shorter than    3475 bp
  25% of sequence in fragments shorter than    4068 bp
  30% of sequence in fragments shorter than    4663 bp
  35% of sequence in fragments shorter than    5266 bp
  40% of sequence in fragments shorter than    5893 bp
  45% of sequence in fragments shorter than    6546 bp
  50% of sequence in fragments shorter than    7236 bp
  55% of sequence in fragments shorter than    7982 bp
  60% of sequence in fragments shorter than    8797 bp
  65% of sequence in fragments shorter than    9704 bp
  70% of sequence in fragments shorter than   10733 bp
  75% of sequence in fragments shorter than   11914 bp
  80% of sequence in fragments shorter than   13387 bp
  85% of sequence in fragments shorter than   15281 bp
  90% of sequence in fragments shorter than   18027 bp
  95% of sequence in fragments shorter than   23127 bp

# AflII.SacII (CTTAAG+CCGCGG) double digest, 709 thousand fragments (643284+66901 sites), 4198 bp average fragment length
   5% of sequence in fragments shorter than    1497 bp
  10% of sequence in fragments shorter than    2274 bp
  15% of sequence in fragments shorter than    2935 bp
  20% of sequence in fragments shorter than    3569 bp
  25% of sequence in fragments shorter than    4169 bp
  30% of sequence in fragments shorter than    4767 bp
  35% of sequence in fragments shorter than    5374 bp
  40% of sequence in fragments shorter than    6002 bp
  45% of sequence in fragments shorter than    6662 bp
  50% of sequence in fragments shorter than    7368 bp
  55% of sequence in fragments shorter than    8111 bp
  60% of sequence in fragments shorter than    8938 bp
  65% of sequence in fragments shorter than    9851 bp
  70% of sequence in fragments shorter than   10900 bp
  75% of sequence in fragments shorter than   12129 bp
  80% of sequence in fragments shorter than   13615 bp
  85% of sequence in fragments shorter than   15568 bp
  90% of sequence in fragments shorter than   18408 bp
  95% of sequence in fragments shorter than   24654 bp

# AscI.BceAI (GGCGCGCC+ACGGC) double digest, 709 thousand fragments (4751+705178 sites), 4200 bp average fragment length
   5% of sequence in fragments shorter than    1374 bp
  10% of sequence in fragments shorter than    2265 bp
  15% of sequence in fragments shorter than    3090 bp
  20% of sequence in fragments shorter than    3896 bp
  25% of sequence in fragments shorter than    4710 bp
  30% of sequence in fragments shorter than    5548 bp
  35% of sequence in fragments shorter than    6409 bp
  40% of sequence in fragments shorter than    7332 bp
  45% of sequence in fragments shorter than    8312 bp
  50% of sequence in fragments shorter than    9359 bp
  55% of sequence in fragments shorter than   10514 bp
  60% of sequence in fragments shorter than   11799 bp
  65% of sequence in fragments shorter than   13231 bp
  70% of sequence in fragments shorter than   14898 bp
  75% of sequence in fragments shorter than   16842 bp
  80% of sequence in fragments shorter than   19249 bp
  85% of sequence in fragments shorter than   22391 bp
  90% of sequence in fragments shorter than   26881 bp
  95% of sequence in fragments shorter than   35374 bp

# BciVI.FseI (GTATCC+GGCCGGCC) double digest, 709 thousand fragments (696093+13662 sites), 4201 bp average fragment length
   5% of sequence in fragments shorter than    1493 bp
  10% of sequence in fragments shorter than    2206 bp
  15% of sequence in fragments shorter than    2865 bp
  20% of sequence in fragments shorter than    3481 bp
  25% of sequence in fragments shorter than    4074 bp
  30% of sequence in fragments shorter than    4668 bp
  35% of sequence in fragments shorter than    5271 bp
  40% of sequence in fragments shorter than    5895 bp
  45% of sequence in fragments shorter than    6546 bp
  50% of sequence in fragments shorter than    7231 bp
  55% of sequence in fragments shorter than    7976 bp
  60% of sequence in fragments shorter than    8786 bp
  65% of sequence in fragments shorter than    9684 bp
  70% of sequence in fragments shorter than   10707 bp
  75% of sequence in fragments shorter than   11884 bp
  80% of sequence in fragments shorter than   13349 bp
  85% of sequence in fragments shorter than   15230 bp
  90% of sequence in fragments shorter than   17958 bp
  95% of sequence in fragments shorter than   23065 bp

# Hpy99I.SphI (CGWCG+GCATGC) double digest, 708 thousand fragments (157917+550952 sites), 4206 bp average fragment length
   5% of sequence in fragments shorter than    1467 bp
  10% of sequence in fragments shorter than    2255 bp
  15% of sequence in fragments shorter than    2950 bp
  20% of sequence in fragments shorter than    3610 bp
  25% of sequence in fragments shorter than    4264 bp
  30% of sequence in fragments shorter than    4931 bp
  35% of sequence in fragments shorter than    5612 bp
  40% of sequence in fragments shorter than    6319 bp
  45% of sequence in fragments shorter than    7060 bp
  50% of sequence in fragments shorter than    7836 bp
  55% of sequence in fragments shorter than    8697 bp
  60% of sequence in fragments shorter than    9644 bp
  65% of sequence in fragments shorter than   10718 bp
  70% of sequence in fragments shorter than   11940 bp
  75% of sequence in fragments shorter than   13365 bp
  80% of sequence in fragments shorter than   15147 bp
  85% of sequence in fragments shorter than   17444 bp
  90% of sequence in fragments shorter than   20811 bp
  95% of sequence in fragments shorter than   27277 bp

# BciVI.NotI (GTATCC+GCGGCCGC) double digest, 705 thousand fragments (696093+9795 sites), 4224 bp average fragment length
   5% of sequence in fragments shorter than    1501 bp
  10% of sequence in fragments shorter than    2219 bp
  15% of sequence in fragments shorter than    2884 bp
  20% of sequence in fragments shorter than    3504 bp
  25% of sequence in fragments shorter than    4101 bp
  30% of sequence in fragments shorter than    4698 bp
  35% of sequence in fragments shorter than    5303 bp
  40% of sequence in fragments shorter than    5932 bp
  45% of sequence in fragments shorter than    6588 bp
  50% of sequence in fragments shorter than    7280 bp
  55% of sequence in fragments shorter than    8032 bp
  60% of sequence in fragments shorter than    8851 bp
  65% of sequence in fragments shorter than    9763 bp
  70% of sequence in fragments shorter than   10795 bp
  75% of sequence in fragments shorter than   11988 bp
  80% of sequence in fragments shorter than   13473 bp
  85% of sequence in fragments shorter than   15376 bp
  90% of sequence in fragments shorter than   18166 bp
  95% of sequence in fragments shorter than   23429 bp

# BciVI.RsrII (GTATCC+CGGWCCG) double digest, 705 thousand fragments (696093+9764 sites), 4224 bp average fragment length
   5% of sequence in fragments shorter than    1500 bp
  10% of sequence in fragments shorter than    2216 bp
  15% of sequence in fragments shorter than    2881 bp
  20% of sequence in fragments shorter than    3500 bp
  25% of sequence in fragments shorter than    4099 bp
  30% of sequence in fragments shorter than    4697 bp
  35% of sequence in fragments shorter than    5301 bp
  40% of sequence in fragments shorter than    5931 bp
  45% of sequence in fragments shorter than    6587 bp
  50% of sequence in fragments shorter than    7280 bp
  55% of sequence in fragments shorter than    8031 bp
  60% of sequence in fragments shorter than    8850 bp
  65% of sequence in fragments shorter than    9759 bp
  70% of sequence in fragments shorter than   10790 bp
  75% of sequence in fragments shorter than   11979 bp
  80% of sequence in fragments shorter than   13466 bp
  85% of sequence in fragments shorter than   15375 bp
  90% of sequence in fragments shorter than   18166 bp
  95% of sequence in fragments shorter than   23432 bp

# HgaI.SnaBI (GACGC+TACGTA) double digest, 703 thousand fragments (571889+132299 sites), 4234 bp average fragment length
   5% of sequence in fragments shorter than    1438 bp
  10% of sequence in fragments shorter than    2360 bp
  15% of sequence in fragments shorter than    3159 bp
  20% of sequence in fragments shorter than    3955 bp
  25% of sequence in fragments shorter than    4756 bp
  30% of sequence in fragments shorter than    5563 bp
  35% of sequence in fragments shorter than    6397 bp
  40% of sequence in fragments shorter than    7247 bp
  45% of sequence in fragments shorter than    8165 bp
  50% of sequence in fragments shorter than    9142 bp
  55% of sequence in fragments shorter than   10215 bp
  60% of sequence in fragments shorter than   11400 bp
  65% of sequence in fragments shorter than   12707 bp
  70% of sequence in fragments shorter than   14231 bp
  75% of sequence in fragments shorter than   15992 bp
  80% of sequence in fragments shorter than   18135 bp
  85% of sequence in fragments shorter than   20950 bp
  90% of sequence in fragments shorter than   24963 bp
  95% of sequence in fragments shorter than   32628 bp

# EciI.SnaBI (GGCGGA+TACGTA) double digest, 703 thousand fragments (571273+132299 sites), 4237 bp average fragment length
   5% of sequence in fragments shorter than    1395 bp
  10% of sequence in fragments shorter than    2262 bp
  15% of sequence in fragments shorter than    3033 bp
  20% of sequence in fragments shorter than    3799 bp
  25% of sequence in fragments shorter than    4590 bp
  30% of sequence in fragments shorter than    5412 bp
  35% of sequence in fragments shorter than    6277 bp
  40% of sequence in fragments shorter than    7189 bp
  45% of sequence in fragments shorter than    8180 bp
  50% of sequence in fragments shorter than    9246 bp
  55% of sequence in fragments shorter than   10450 bp
  60% of sequence in fragments shorter than   11781 bp
  65% of sequence in fragments shorter than   13286 bp
  70% of sequence in fragments shorter than   15025 bp
  75% of sequence in fragments shorter than   17086 bp
  80% of sequence in fragments shorter than   19617 bp
  85% of sequence in fragments shorter than   22972 bp
  90% of sequence in fragments shorter than   27897 bp
  95% of sequence in fragments shorter than   38038 bp

# PacI.ScaI (TTAATTAA+AGTACT) double digest, 702 thousand fragments (159455+543793 sites), 4239 bp average fragment length
   5% of sequence in fragments shorter than    1474 bp
  10% of sequence in fragments shorter than    2239 bp
  15% of sequence in fragments shorter than    2904 bp
  20% of sequence in fragments shorter than    3543 bp
  25% of sequence in fragments shorter than    4172 bp
  30% of sequence in fragments shorter than    4803 bp
  35% of sequence in fragments shorter than    5453 bp
  40% of sequence in fragments shorter than    6137 bp
  45% of sequence in fragments shorter than    6856 bp
  50% of sequence in fragments shorter than    7626 bp
  55% of sequence in fragments shorter than    8457 bp
  60% of sequence in fragments shorter than    9384 bp
  65% of sequence in fragments shorter than   10411 bp
  70% of sequence in fragments shorter than   11616 bp
  75% of sequence in fragments shorter than   13051 bp
  80% of sequence in fragments shorter than   14858 bp
  85% of sequence in fragments shorter than   17288 bp
  90% of sequence in fragments shorter than   21122 bp
  95% of sequence in fragments shorter than   29741 bp

# AflII.PspXI (CTTAAG+VCTCGAGB) double digest, 702 thousand fragments (643284+59193 sites), 4244 bp average fragment length
   5% of sequence in fragments shorter than    1499 bp
  10% of sequence in fragments shorter than    2252 bp
  15% of sequence in fragments shorter than    2895 bp
  20% of sequence in fragments shorter than    3508 bp
  25% of sequence in fragments shorter than    4084 bp
  30% of sequence in fragments shorter than    4672 bp
  35% of sequence in fragments shorter than    5259 bp
  40% of sequence in fragments shorter than    5873 bp
  45% of sequence in fragments shorter than    6519 bp
  50% of sequence in fragments shorter than    7204 bp
  55% of sequence in fragments shorter than    7943 bp
  60% of sequence in fragments shorter than    8741 bp
  65% of sequence in fragments shorter than    9635 bp
  70% of sequence in fragments shorter than   10663 bp
  75% of sequence in fragments shorter than   11848 bp
  80% of sequence in fragments shorter than   13308 bp
  85% of sequence in fragments shorter than   15245 bp
  90% of sequence in fragments shorter than   18065 bp
  95% of sequence in fragments shorter than   24102 bp

# BamHI.BstEII (GGATCC+GGTNACC) double digest, 701 thousand fragments (365999+335865 sites), 4247 bp average fragment length
   5% of sequence in fragments shorter than    1473 bp
  10% of sequence in fragments shorter than    2241 bp
  15% of sequence in fragments shorter than    2927 bp
  20% of sequence in fragments shorter than    3490 bp
  25% of sequence in fragments shorter than    4122 bp
  30% of sequence in fragments shorter than    4769 bp
  35% of sequence in fragments shorter than    5424 bp
  40% of sequence in fragments shorter than    6106 bp
  45% of sequence in fragments shorter than    6820 bp
  50% of sequence in fragments shorter than    7594 bp
  55% of sequence in fragments shorter than    8437 bp
  60% of sequence in fragments shorter than    9359 bp
  65% of sequence in fragments shorter than   10392 bp
  70% of sequence in fragments shorter than   11565 bp
  75% of sequence in fragments shorter than   12943 bp
  80% of sequence in fragments shorter than   14679 bp
  85% of sequence in fragments shorter than   16888 bp
  90% of sequence in fragments shorter than   20208 bp
  95% of sequence in fragments shorter than   26765 bp

# Hpy99I.ScaI (CGWCG+AGTACT) double digest, 701 thousand fragments (157917+543793 sites), 4249 bp average fragment length
   5% of sequence in fragments shorter than    1506 bp
  10% of sequence in fragments shorter than    2293 bp
  15% of sequence in fragments shorter than    2971 bp
  20% of sequence in fragments shorter than    3621 bp
  25% of sequence in fragments shorter than    4256 bp
  30% of sequence in fragments shorter than    4891 bp
  35% of sequence in fragments shorter than    5540 bp
  40% of sequence in fragments shorter than    6207 bp
  45% of sequence in fragments shorter than    6901 bp
  50% of sequence in fragments shorter than    7634 bp
  55% of sequence in fragments shorter than    8413 bp
  60% of sequence in fragments shorter than    9264 bp
  65% of sequence in fragments shorter than   10210 bp
  70% of sequence in fragments shorter than   11299 bp
  75% of sequence in fragments shorter than   12559 bp
  80% of sequence in fragments shorter than   14117 bp
  85% of sequence in fragments shorter than   16121 bp
  90% of sequence in fragments shorter than   19006 bp
  95% of sequence in fragments shorter than   24673 bp

# AatII.HaeII (GACGTC+RGCGCY) double digest, 700 thousand fragments (71089+630101 sites), 4252 bp average fragment length
   5% of sequence in fragments shorter than    1278 bp
  10% of sequence in fragments shorter than    2344 bp
  15% of sequence in fragments shorter than    3385 bp
  20% of sequence in fragments shorter than    4394 bp
  25% of sequence in fragments shorter than    5425 bp
  30% of sequence in fragments shorter than    6497 bp
  35% of sequence in fragments shorter than    7622 bp
  40% of sequence in fragments shorter than    8838 bp
  45% of sequence in fragments shorter than   10130 bp
  50% of sequence in fragments shorter than   11546 bp
  55% of sequence in fragments shorter than   13085 bp
  60% of sequence in fragments shorter than   14853 bp
  65% of sequence in fragments shorter than   16811 bp
  70% of sequence in fragments shorter than   19090 bp
  75% of sequence in fragments shorter than   21855 bp
  80% of sequence in fragments shorter than   25112 bp
  85% of sequence in fragments shorter than   29435 bp
  90% of sequence in fragments shorter than   35405 bp
  95% of sequence in fragments shorter than   46013 bp

# AscI.BciVI (GGCGCGCC+GTATCC) double digest, 700 thousand fragments (4751+696093 sites), 4254 bp average fragment length
   5% of sequence in fragments shorter than    1510 bp
  10% of sequence in fragments shorter than    2231 bp
  15% of sequence in fragments shorter than    2902 bp
  20% of sequence in fragments shorter than    3523 bp
  25% of sequence in fragments shorter than    4128 bp
  30% of sequence in fragments shorter than    4728 bp
  35% of sequence in fragments shorter than    5339 bp
  40% of sequence in fragments shorter than    5971 bp
  45% of sequence in fragments shorter than    6634 bp
  50% of sequence in fragments shorter than    7332 bp
  55% of sequence in fragments shorter than    8092 bp
  60% of sequence in fragments shorter than    8919 bp
  65% of sequence in fragments shorter than    9839 bp
  70% of sequence in fragments shorter than   10881 bp
  75% of sequence in fragments shorter than   12088 bp
  80% of sequence in fragments shorter than   13586 bp
  85% of sequence in fragments shorter than   15537 bp
  90% of sequence in fragments shorter than   18371 bp
  95% of sequence in fragments shorter than   23730 bp

# SexAI.SpeI (ACCWGGT+ACTAGT) double digest, 697 thousand fragments (297900+400286 sites), 4270 bp average fragment length
   5% of sequence in fragments shorter than    1502 bp
  10% of sequence in fragments shorter than    2250 bp
  15% of sequence in fragments shorter than    2904 bp
  20% of sequence in fragments shorter than    3522 bp
  25% of sequence in fragments shorter than    4116 bp
  30% of sequence in fragments shorter than    4716 bp
  35% of sequence in fragments shorter than    5321 bp
  40% of sequence in fragments shorter than    5923 bp
  45% of sequence in fragments shorter than    6568 bp
  50% of sequence in fragments shorter than    7247 bp
  55% of sequence in fragments shorter than    7981 bp
  60% of sequence in fragments shorter than    8781 bp
  65% of sequence in fragments shorter than    9663 bp
  70% of sequence in fragments shorter than   10671 bp
  75% of sequence in fragments shorter than   11851 bp
  80% of sequence in fragments shorter than   13276 bp
  85% of sequence in fragments shorter than   15139 bp
  90% of sequence in fragments shorter than   17799 bp
  95% of sequence in fragments shorter than   23023 bp

# HgaI.NaeI (GACGC+GCCGGC) double digest, 697 thousand fragments (571889+125692 sites), 4274 bp average fragment length
   5% of sequence in fragments shorter than    1436 bp
  10% of sequence in fragments shorter than    2479 bp
  15% of sequence in fragments shorter than    3435 bp
  20% of sequence in fragments shorter than    4403 bp
  25% of sequence in fragments shorter than    5392 bp
  30% of sequence in fragments shorter than    6397 bp
  35% of sequence in fragments shorter than    7443 bp
  40% of sequence in fragments shorter than    8581 bp
  45% of sequence in fragments shorter than    9796 bp
  50% of sequence in fragments shorter than   11117 bp
  55% of sequence in fragments shorter than   12554 bp
  60% of sequence in fragments shorter than   14187 bp
  65% of sequence in fragments shorter than   15996 bp
  70% of sequence in fragments shorter than   18060 bp
  75% of sequence in fragments shorter than   20555 bp
  80% of sequence in fragments shorter than   23583 bp
  85% of sequence in fragments shorter than   27495 bp
  90% of sequence in fragments shorter than   33536 bp
  95% of sequence in fragments shorter than   44880 bp

# EciI.NaeI (GGCGGA+GCCGGC) double digest, 696 thousand fragments (571273+125692 sites), 4277 bp average fragment length
   5% of sequence in fragments shorter than    1383 bp
  10% of sequence in fragments shorter than    2316 bp
  15% of sequence in fragments shorter than    3193 bp
  20% of sequence in fragments shorter than    4103 bp
  25% of sequence in fragments shorter than    5064 bp
  30% of sequence in fragments shorter than    6099 bp
  35% of sequence in fragments shorter than    7204 bp
  40% of sequence in fragments shorter than    8411 bp
  45% of sequence in fragments shorter than    9756 bp
  50% of sequence in fragments shorter than   11241 bp
  55% of sequence in fragments shorter than   12904 bp
  60% of sequence in fragments shorter than   14840 bp
  65% of sequence in fragments shorter than   17046 bp
  70% of sequence in fragments shorter than   19617 bp
  75% of sequence in fragments shorter than   22690 bp
  80% of sequence in fragments shorter than   26561 bp
  85% of sequence in fragments shorter than   31675 bp
  90% of sequence in fragments shorter than   39646 bp
  95% of sequence in fragments shorter than   55828 bp

# HaeII.SacII (RGCGCY+CCGCGG) double digest, 696 thousand fragments (630101+66901 sites), 4278 bp average fragment length
   5% of sequence in fragments shorter than    1339 bp
  10% of sequence in fragments shorter than    2517 bp
  15% of sequence in fragments shorter than    3675 bp
  20% of sequence in fragments shorter than    4820 bp
  25% of sequence in fragments shorter than    5985 bp
  30% of sequence in fragments shorter than    7200 bp
  35% of sequence in fragments shorter than    8503 bp
  40% of sequence in fragments shorter than    9893 bp
  45% of sequence in fragments shorter than   11378 bp
  50% of sequence in fragments shorter than   13010 bp
  55% of sequence in fragments shorter than   14846 bp
  60% of sequence in fragments shorter than   16857 bp
  65% of sequence in fragments shorter than   19195 bp
  70% of sequence in fragments shorter than   21888 bp
  75% of sequence in fragments shorter than   25002 bp
  80% of sequence in fragments shorter than   28875 bp
  85% of sequence in fragments shorter than   33842 bp
  90% of sequence in fragments shorter than   40929 bp
  95% of sequence in fragments shorter than   53477 bp

# AflII.AgeI (CTTAAG+ACCGGT) double digest, 696 thousand fragments (643284+53386 sites), 4279 bp average fragment length
   5% of sequence in fragments shorter than    1510 bp
  10% of sequence in fragments shorter than    2270 bp
  15% of sequence in fragments shorter than    2892 bp
  20% of sequence in fragments shorter than    3514 bp
  25% of sequence in fragments shorter than    4112 bp
  30% of sequence in fragments shorter than    4707 bp
  35% of sequence in fragments shorter than    5309 bp
  40% of sequence in fragments shorter than    5927 bp
  45% of sequence in fragments shorter than    6591 bp
  50% of sequence in fragments shorter than    7294 bp
  55% of sequence in fragments shorter than    8041 bp
  60% of sequence in fragments shorter than    8867 bp
  65% of sequence in fragments shorter than    9780 bp
  70% of sequence in fragments shorter than   10833 bp
  75% of sequence in fragments shorter than   12062 bp
  80% of sequence in fragments shorter than   13566 bp
  85% of sequence in fragments shorter than   15551 bp
  90% of sequence in fragments shorter than   18494 bp
  95% of sequence in fragments shorter than   24879 bp

# MfeI.SnaBI (CAATTG+TACGTA) double digest, 696 thousand fragments (564303+132299 sites), 4280 bp average fragment length
   5% of sequence in fragments shorter than    1472 bp
  10% of sequence in fragments shorter than    2230 bp
  15% of sequence in fragments shorter than    2899 bp
  20% of sequence in fragments shorter than    3529 bp
  25% of sequence in fragments shorter than    4157 bp
  30% of sequence in fragments shorter than    4776 bp
  35% of sequence in fragments shorter than    5408 bp
  40% of sequence in fragments shorter than    6073 bp
  45% of sequence in fragments shorter than    6762 bp
  50% of sequence in fragments shorter than    7504 bp
  55% of sequence in fragments shorter than    8324 bp
  60% of sequence in fragments shorter than    9226 bp
  65% of sequence in fragments shorter than   10256 bp
  70% of sequence in fragments shorter than   11429 bp
  75% of sequence in fragments shorter than   12808 bp
  80% of sequence in fragments shorter than   14531 bp
  85% of sequence in fragments shorter than   16845 bp
  90% of sequence in fragments shorter than   20474 bp
  95% of sequence in fragments shorter than   28060 bp

# ApaI.BsrBI (GGGCCC+CCGCTC) double digest, 695 thousand fragments (460339+235523 sites), 4285 bp average fragment length
   5% of sequence in fragments shorter than    1348 bp
  10% of sequence in fragments shorter than    2376 bp
  15% of sequence in fragments shorter than    3380 bp
  20% of sequence in fragments shorter than    4393 bp
  25% of sequence in fragments shorter than    5456 bp
  30% of sequence in fragments shorter than    6558 bp
  35% of sequence in fragments shorter than    7723 bp
  40% of sequence in fragments shorter than    8938 bp
  45% of sequence in fragments shorter than   10231 bp
  50% of sequence in fragments shorter than   11646 bp
  55% of sequence in fragments shorter than   13202 bp
  60% of sequence in fragments shorter than   14973 bp
  65% of sequence in fragments shorter than   16956 bp
  70% of sequence in fragments shorter than   19243 bp
  75% of sequence in fragments shorter than   21977 bp
  80% of sequence in fragments shorter than   25301 bp
  85% of sequence in fragments shorter than   29758 bp
  90% of sequence in fragments shorter than   36290 bp
  95% of sequence in fragments shorter than   48740 bp

# HgaI.XhoI (GACGC+CTCGAG) double digest, 692 thousand fragments (571889+121308 sites), 4301 bp average fragment length
   5% of sequence in fragments shorter than    1398 bp
  10% of sequence in fragments shorter than    2358 bp
  15% of sequence in fragments shorter than    3245 bp
  20% of sequence in fragments shorter than    4114 bp
  25% of sequence in fragments shorter than    5006 bp
  30% of sequence in fragments shorter than    5901 bp
  35% of sequence in fragments shorter than    6841 bp
  40% of sequence in fragments shorter than    7832 bp
  45% of sequence in fragments shorter than    8911 bp
  50% of sequence in fragments shorter than   10079 bp
  55% of sequence in fragments shorter than   11346 bp
  60% of sequence in fragments shorter than   12767 bp
  65% of sequence in fragments shorter than   14356 bp
  70% of sequence in fragments shorter than   16213 bp
  75% of sequence in fragments shorter than   18383 bp
  80% of sequence in fragments shorter than   21038 bp
  85% of sequence in fragments shorter than   24495 bp
  90% of sequence in fragments shorter than   29654 bp
  95% of sequence in fragments shorter than   39558 bp

# EciI.XhoI (GGCGGA+CTCGAG) double digest, 692 thousand fragments (571273+121308 sites), 4304 bp average fragment length
   5% of sequence in fragments shorter than    1392 bp
  10% of sequence in fragments shorter than    2283 bp
  15% of sequence in fragments shorter than    3110 bp
  20% of sequence in fragments shorter than    3942 bp
  25% of sequence in fragments shorter than    4802 bp
  30% of sequence in fragments shorter than    5705 bp
  35% of sequence in fragments shorter than    6663 bp
  40% of sequence in fragments shorter than    7697 bp
  45% of sequence in fragments shorter than    8814 bp
  50% of sequence in fragments shorter than   10072 bp
  55% of sequence in fragments shorter than   11476 bp
  60% of sequence in fragments shorter than   13034 bp
  65% of sequence in fragments shorter than   14867 bp
  70% of sequence in fragments shorter than   16960 bp
  75% of sequence in fragments shorter than   19474 bp
  80% of sequence in fragments shorter than   22628 bp
  85% of sequence in fragments shorter than   26904 bp
  90% of sequence in fragments shorter than   33218 bp
  95% of sequence in fragments shorter than   46072 bp

# ApaI.KasI (GGGCCC+GGCGCC) double digest, 690 thousand fragments (460339+230381 sites), 4316 bp average fragment length
   5% of sequence in fragments shorter than    1361 bp
  10% of sequence in fragments shorter than    2337 bp
  15% of sequence in fragments shorter than    3267 bp
  20% of sequence in fragments shorter than    4209 bp
  25% of sequence in fragments shorter than    5183 bp
  30% of sequence in fragments shorter than    6204 bp
  35% of sequence in fragments shorter than    7282 bp
  40% of sequence in fragments shorter than    8424 bp
  45% of sequence in fragments shorter than    9647 bp
  50% of sequence in fragments shorter than   10981 bp
  55% of sequence in fragments shorter than   12474 bp
  60% of sequence in fragments shorter than   14115 bp
  65% of sequence in fragments shorter than   15984 bp
  70% of sequence in fragments shorter than   18174 bp
  75% of sequence in fragments shorter than   20816 bp
  80% of sequence in fragments shorter than   24136 bp
  85% of sequence in fragments shorter than   28555 bp
  90% of sequence in fragments shorter than   35515 bp
  95% of sequence in fragments shorter than   49565 bp

# MfeI.NaeI (CAATTG+GCCGGC) double digest, 689 thousand fragments (564303+125692 sites), 4321 bp average fragment length
   5% of sequence in fragments shorter than    1526 bp
  10% of sequence in fragments shorter than    2318 bp
  15% of sequence in fragments shorter than    3011 bp
  20% of sequence in fragments shorter than    3659 bp
  25% of sequence in fragments shorter than    4288 bp
  30% of sequence in fragments shorter than    4906 bp
  35% of sequence in fragments shorter than    5536 bp
  40% of sequence in fragments shorter than    6192 bp
  45% of sequence in fragments shorter than    6873 bp
  50% of sequence in fragments shorter than    7593 bp
  55% of sequence in fragments shorter than    8376 bp
  60% of sequence in fragments shorter than    9222 bp
  65% of sequence in fragments shorter than   10179 bp
  70% of sequence in fragments shorter than   11243 bp
  75% of sequence in fragments shorter than   12503 bp
  80% of sequence in fragments shorter than   14029 bp
  85% of sequence in fragments shorter than   15996 bp
  90% of sequence in fragments shorter than   18824 bp
  95% of sequence in fragments shorter than   24219 bp

# ClaI.SacI (ATCGAT+GAGCTC) double digest, 689 thousand fragments (86477+603323 sites), 4322 bp average fragment length
   5% of sequence in fragments shorter than    1484 bp
  10% of sequence in fragments shorter than    2253 bp
  15% of sequence in fragments shorter than    2949 bp
  20% of sequence in fragments shorter than    3608 bp
  25% of sequence in fragments shorter than    4262 bp
  30% of sequence in fragments shorter than    4905 bp
  35% of sequence in fragments shorter than    5570 bp
  40% of sequence in fragments shorter than    6266 bp
  45% of sequence in fragments shorter than    6993 bp
  50% of sequence in fragments shorter than    7764 bp
  55% of sequence in fragments shorter than    8598 bp
  60% of sequence in fragments shorter than    9523 bp
  65% of sequence in fragments shorter than   10560 bp
  70% of sequence in fragments shorter than   11725 bp
  75% of sequence in fragments shorter than   13093 bp
  80% of sequence in fragments shorter than   14742 bp
  85% of sequence in fragments shorter than   16929 bp
  90% of sequence in fragments shorter than   20195 bp
  95% of sequence in fragments shorter than   26597 bp

# HaeII.PspXI (RGCGCY+VCTCGAGB) double digest, 688 thousand fragments (630101+59193 sites), 4326 bp average fragment length
   5% of sequence in fragments shorter than    1301 bp
  10% of sequence in fragments shorter than    2389 bp
  15% of sequence in fragments shorter than    3449 bp
  20% of sequence in fragments shorter than    4483 bp
  25% of sequence in fragments shorter than    5552 bp
  30% of sequence in fragments shorter than    6659 bp
  35% of sequence in fragments shorter than    7825 bp
  40% of sequence in fragments shorter than    9075 bp
  45% of sequence in fragments shorter than   10416 bp
  50% of sequence in fragments shorter than   11885 bp
  55% of sequence in fragments shorter than   13514 bp
  60% of sequence in fragments shorter than   15351 bp
  65% of sequence in fragments shorter than   17407 bp
  70% of sequence in fragments shorter than   19837 bp
  75% of sequence in fragments shorter than   22679 bp
  80% of sequence in fragments shorter than   26057 bp
  85% of sequence in fragments shorter than   30606 bp
  90% of sequence in fragments shorter than   36838 bp
  95% of sequence in fragments shorter than   48000 bp

# KpnI.SpeI (GGTACC+ACTAGT) double digest, 687 thousand fragments (287974+400286 sites), 4332 bp average fragment length
   5% of sequence in fragments shorter than    1456 bp
  10% of sequence in fragments shorter than    2189 bp
  15% of sequence in fragments shorter than    2875 bp
  20% of sequence in fragments shorter than    3522 bp
  25% of sequence in fragments shorter than    4145 bp
  30% of sequence in fragments shorter than    4782 bp
  35% of sequence in fragments shorter than    5428 bp
  40% of sequence in fragments shorter than    6093 bp
  45% of sequence in fragments shorter than    6787 bp
  50% of sequence in fragments shorter than    7520 bp
  55% of sequence in fragments shorter than    8323 bp
  60% of sequence in fragments shorter than    9187 bp
  65% of sequence in fragments shorter than   10152 bp
  70% of sequence in fragments shorter than   11270 bp
  75% of sequence in fragments shorter than   12541 bp
  80% of sequence in fragments shorter than   14133 bp
  85% of sequence in fragments shorter than   16176 bp
  90% of sequence in fragments shorter than   19336 bp
  95% of sequence in fragments shorter than   25500 bp

# NheI.SpeI (GCTAGC+ACTAGT) double digest, 687 thousand fragments (287384+400286 sites), 4335 bp average fragment length
   5% of sequence in fragments shorter than    1502 bp
  10% of sequence in fragments shorter than    2228 bp
  15% of sequence in fragments shorter than    2874 bp
  20% of sequence in fragments shorter than    3539 bp
  25% of sequence in fragments shorter than    4188 bp
  30% of sequence in fragments shorter than    4844 bp
  35% of sequence in fragments shorter than    5504 bp
  40% of sequence in fragments shorter than    6190 bp
  45% of sequence in fragments shorter than    6912 bp
  50% of sequence in fragments shorter than    7679 bp
  55% of sequence in fragments shorter than    8517 bp
  60% of sequence in fragments shorter than    9422 bp
  65% of sequence in fragments shorter than   10435 bp
  70% of sequence in fragments shorter than   11603 bp
  75% of sequence in fragments shorter than   12949 bp
  80% of sequence in fragments shorter than   14661 bp
  85% of sequence in fragments shorter than   16929 bp
  90% of sequence in fragments shorter than   20368 bp
  95% of sequence in fragments shorter than   27315 bp

# HpaI.SexAI (GTTAAC+ACCWGGT) double digest, 686 thousand fragments (389418+297900 sites), 4337 bp average fragment length
   5% of sequence in fragments shorter than    1525 bp
  10% of sequence in fragments shorter than    2300 bp
  15% of sequence in fragments shorter than    2964 bp
  20% of sequence in fragments shorter than    3596 bp
  25% of sequence in fragments shorter than    4206 bp
  30% of sequence in fragments shorter than    4823 bp
  35% of sequence in fragments shorter than    5442 bp
  40% of sequence in fragments shorter than    6078 bp
  45% of sequence in fragments shorter than    6750 bp
  50% of sequence in fragments shorter than    7461 bp
  55% of sequence in fragments shorter than    8218 bp
  60% of sequence in fragments shorter than    9045 bp
  65% of sequence in fragments shorter than    9968 bp
  70% of sequence in fragments shorter than   11009 bp
  75% of sequence in fragments shorter than   12246 bp
  80% of sequence in fragments shorter than   13699 bp
  85% of sequence in fragments shorter than   15617 bp
  90% of sequence in fragments shorter than   18469 bp
  95% of sequence in fragments shorter than   24145 bp

# ApaI.SwaI (GGGCCC+ATTTAAAT) double digest, 686 thousand fragments (460339+226602 sites), 4339 bp average fragment length
   5% of sequence in fragments shorter than    1452 bp
  10% of sequence in fragments shorter than    2339 bp
  15% of sequence in fragments shorter than    3131 bp
  20% of sequence in fragments shorter than    3901 bp
  25% of sequence in fragments shorter than    4666 bp
  30% of sequence in fragments shorter than    5434 bp
  35% of sequence in fragments shorter than    6236 bp
  40% of sequence in fragments shorter than    7065 bp
  45% of sequence in fragments shorter than    7936 bp
  50% of sequence in fragments shorter than    8865 bp
  55% of sequence in fragments shorter than    9844 bp
  60% of sequence in fragments shorter than   10942 bp
  65% of sequence in fragments shorter than   12142 bp
  70% of sequence in fragments shorter than   13523 bp
  75% of sequence in fragments shorter than   15139 bp
  80% of sequence in fragments shorter than   17126 bp
  85% of sequence in fragments shorter than   19791 bp
  90% of sequence in fragments shorter than   23668 bp
  95% of sequence in fragments shorter than   31848 bp

# MfeI.XhoI (CAATTG+CTCGAG) double digest, 685 thousand fragments (564303+121308 sites), 4349 bp average fragment length
   5% of sequence in fragments shorter than    1530 bp
  10% of sequence in fragments shorter than    2301 bp
  15% of sequence in fragments shorter than    2930 bp
  20% of sequence in fragments shorter than    3554 bp
  25% of sequence in fragments shorter than    4170 bp
  30% of sequence in fragments shorter than    4782 bp
  35% of sequence in fragments shorter than    5403 bp
  40% of sequence in fragments shorter than    6051 bp
  45% of sequence in fragments shorter than    6728 bp
  50% of sequence in fragments shorter than    7440 bp
  55% of sequence in fragments shorter than    8202 bp
  60% of sequence in fragments shorter than    9043 bp
  65% of sequence in fragments shorter than    9987 bp
  70% of sequence in fragments shorter than   11067 bp
  75% of sequence in fragments shorter than   12319 bp
  80% of sequence in fragments shorter than   13823 bp
  85% of sequence in fragments shorter than   15771 bp
  90% of sequence in fragments shorter than   18631 bp
  95% of sequence in fragments shorter than   23977 bp

# AflII.PmeI (CTTAAG+GTTTAAAC) double digest, 685 thousand fragments (643284+42094 sites), 4350 bp average fragment length
   5% of sequence in fragments shorter than    1526 bp
  10% of sequence in fragments shorter than    2296 bp
  15% of sequence in fragments shorter than    2955 bp
  20% of sequence in fragments shorter than    3587 bp
  25% of sequence in fragments shorter than    4189 bp
  30% of sequence in fragments shorter than    4794 bp
  35% of sequence in fragments shorter than    5412 bp
  40% of sequence in fragments shorter than    6050 bp
  45% of sequence in fragments shorter than    6722 bp
  50% of sequence in fragments shorter than    7447 bp
  55% of sequence in fragments shorter than    8218 bp
  60% of sequence in fragments shorter than    9080 bp
  65% of sequence in fragments shorter than   10040 bp
  70% of sequence in fragments shorter than   11140 bp
  75% of sequence in fragments shorter than   12442 bp
  80% of sequence in fragments shorter than   14070 bp
  85% of sequence in fragments shorter than   16227 bp
  90% of sequence in fragments shorter than   19574 bp
  95% of sequence in fragments shorter than   27011 bp

# AgeI.HaeII (ACCGGT+RGCGCY) double digest, 683 thousand fragments (53386+630101 sites), 4362 bp average fragment length
   5% of sequence in fragments shorter than    1321 bp
  10% of sequence in fragments shorter than    2414 bp
  15% of sequence in fragments shorter than    3482 bp
  20% of sequence in fragments shorter than    4527 bp
  25% of sequence in fragments shorter than    5589 bp
  30% of sequence in fragments shorter than    6689 bp
  35% of sequence in fragments shorter than    7841 bp
  40% of sequence in fragments shorter than    9079 bp
  45% of sequence in fragments shorter than   10396 bp
  50% of sequence in fragments shorter than   11832 bp
  55% of sequence in fragments shorter than   13438 bp
  60% of sequence in fragments shorter than   15198 bp
  65% of sequence in fragments shorter than   17186 bp
  70% of sequence in fragments shorter than   19542 bp
  75% of sequence in fragments shorter than   22276 bp
  80% of sequence in fragments shorter than   25563 bp
  85% of sequence in fragments shorter than   29930 bp
  90% of sequence in fragments shorter than   35865 bp
  95% of sequence in fragments shorter than   46688 bp

# SnaBI.SphI (TACGTA+GCATGC) double digest, 682 thousand fragments (132299+550952 sites), 4364 bp average fragment length
   5% of sequence in fragments shorter than    1530 bp
  10% of sequence in fragments shorter than    2318 bp
  15% of sequence in fragments shorter than    2996 bp
  20% of sequence in fragments shorter than    3636 bp
  25% of sequence in fragments shorter than    4269 bp
  30% of sequence in fragments shorter than    4908 bp
  35% of sequence in fragments shorter than    5552 bp
  40% of sequence in fragments shorter than    6218 bp
  45% of sequence in fragments shorter than    6909 bp
  50% of sequence in fragments shorter than    7645 bp
  55% of sequence in fragments shorter than    8429 bp
  60% of sequence in fragments shorter than    9287 bp
  65% of sequence in fragments shorter than   10244 bp
  70% of sequence in fragments shorter than   11341 bp
  75% of sequence in fragments shorter than   12634 bp
  80% of sequence in fragments shorter than   14182 bp
  85% of sequence in fragments shorter than   16182 bp
  90% of sequence in fragments shorter than   19069 bp
  95% of sequence in fragments shorter than   24669 bp

# BsrBI.EcoRV (CCGCTC+GATATC) double digest, 681 thousand fragments (235523+446473 sites), 4372 bp average fragment length
   5% of sequence in fragments shorter than    1530 bp
  10% of sequence in fragments shorter than    2374 bp
  15% of sequence in fragments shorter than    3108 bp
  20% of sequence in fragments shorter than    3797 bp
  25% of sequence in fragments shorter than    4465 bp
  30% of sequence in fragments shorter than    5135 bp
  35% of sequence in fragments shorter than    5807 bp
  40% of sequence in fragments shorter than    6503 bp
  45% of sequence in fragments shorter than    7226 bp
  50% of sequence in fragments shorter than    7986 bp
  55% of sequence in fragments shorter than    8799 bp
  60% of sequence in fragments shorter than    9699 bp
  65% of sequence in fragments shorter than   10702 bp
  70% of sequence in fragments shorter than   11817 bp
  75% of sequence in fragments shorter than   13109 bp
  80% of sequence in fragments shorter than   14680 bp
  85% of sequence in fragments shorter than   16650 bp
  90% of sequence in fragments shorter than   19446 bp
  95% of sequence in fragments shorter than   24412 bp

# AvrII.ClaI (CCTAGG+ATCGAT) double digest, 681 thousand fragments (594956+86477 sites), 4375 bp average fragment length
   5% of sequence in fragments shorter than    1518 bp
  10% of sequence in fragments shorter than    2335 bp
  15% of sequence in fragments shorter than    3032 bp
  20% of sequence in fragments shorter than    3684 bp
  25% of sequence in fragments shorter than    4309 bp
  30% of sequence in fragments shorter than    4935 bp
  35% of sequence in fragments shorter than    5571 bp
  40% of sequence in fragments shorter than    6230 bp
  45% of sequence in fragments shorter than    6915 bp
  50% of sequence in fragments shorter than    7654 bp
  55% of sequence in fragments shorter than    8436 bp
  60% of sequence in fragments shorter than    9301 bp
  65% of sequence in fragments shorter than   10247 bp
  70% of sequence in fragments shorter than   11324 bp
  75% of sequence in fragments shorter than   12608 bp
  80% of sequence in fragments shorter than   14165 bp
  85% of sequence in fragments shorter than   16230 bp
  90% of sequence in fragments shorter than   19185 bp
  95% of sequence in fragments shorter than   25365 bp

# HpaI.KpnI (GTTAAC+GGTACC) double digest, 677 thousand fragments (389418+287974 sites), 4401 bp average fragment length
   5% of sequence in fragments shorter than    1502 bp
  10% of sequence in fragments shorter than    2259 bp
  15% of sequence in fragments shorter than    2946 bp
  20% of sequence in fragments shorter than    3586 bp
  25% of sequence in fragments shorter than    4214 bp
  30% of sequence in fragments shorter than    4851 bp
  35% of sequence in fragments shorter than    5496 bp
  40% of sequence in fragments shorter than    6157 bp
  45% of sequence in fragments shorter than    6849 bp
  50% of sequence in fragments shorter than    7583 bp
  55% of sequence in fragments shorter than    8366 bp
  60% of sequence in fragments shorter than    9211 bp
  65% of sequence in fragments shorter than   10165 bp
  70% of sequence in fragments shorter than   11263 bp
  75% of sequence in fragments shorter than   12542 bp
  80% of sequence in fragments shorter than   14088 bp
  85% of sequence in fragments shorter than   16155 bp
  90% of sequence in fragments shorter than   19116 bp
  95% of sequence in fragments shorter than   25328 bp

# HpaI.NheI (GTTAAC+GCTAGC) double digest, 676 thousand fragments (389418+287384 sites), 4405 bp average fragment length
   5% of sequence in fragments shorter than    1580 bp
  10% of sequence in fragments shorter than    2256 bp
  15% of sequence in fragments shorter than    2930 bp
  20% of sequence in fragments shorter than    3588 bp
  25% of sequence in fragments shorter than    4228 bp
  30% of sequence in fragments shorter than    4875 bp
  35% of sequence in fragments shorter than    5528 bp
  40% of sequence in fragments shorter than    6208 bp
  45% of sequence in fragments shorter than    6920 bp
  50% of sequence in fragments shorter than    7678 bp
  55% of sequence in fragments shorter than    8497 bp
  60% of sequence in fragments shorter than    9378 bp
  65% of sequence in fragments shorter than   10367 bp
  70% of sequence in fragments shorter than   11499 bp
  75% of sequence in fragments shorter than   12802 bp
  80% of sequence in fragments shorter than   14412 bp
  85% of sequence in fragments shorter than   16550 bp
  90% of sequence in fragments shorter than   19701 bp
  95% of sequence in fragments shorter than   26171 bp

# EcoRV.KasI (GATATC+GGCGCC) double digest, 676 thousand fragments (446473+230381 sites), 4405 bp average fragment length
   5% of sequence in fragments shorter than    1544 bp
  10% of sequence in fragments shorter than    2364 bp
  15% of sequence in fragments shorter than    3063 bp
  20% of sequence in fragments shorter than    3743 bp
  25% of sequence in fragments shorter than    4400 bp
  30% of sequence in fragments shorter than    5052 bp
  35% of sequence in fragments shorter than    5712 bp
  40% of sequence in fragments shorter than    6397 bp
  45% of sequence in fragments shorter than    7114 bp
  50% of sequence in fragments shorter than    7884 bp
  55% of sequence in fragments shorter than    8697 bp
  60% of sequence in fragments shorter than    9579 bp
  65% of sequence in fragments shorter than   10563 bp
  70% of sequence in fragments shorter than   11683 bp
  75% of sequence in fragments shorter than   12981 bp
  80% of sequence in fragments shorter than   14549 bp
  85% of sequence in fragments shorter than   16599 bp
  90% of sequence in fragments shorter than   19457 bp
  95% of sequence in fragments shorter than   24745 bp

# NaeI.SphI (GCCGGC+GCATGC) double digest, 676 thousand fragments (125692+550952 sites), 4406 bp average fragment length
   5% of sequence in fragments shorter than    1526 bp
  10% of sequence in fragments shorter than    2343 bp
  15% of sequence in fragments shorter than    3070 bp
  20% of sequence in fragments shorter than    3763 bp
  25% of sequence in fragments shorter than    4453 bp
  30% of sequence in fragments shorter than    5152 bp
  35% of sequence in fragments shorter than    5866 bp
  40% of sequence in fragments shorter than    6614 bp
  45% of sequence in fragments shorter than    7393 bp
  50% of sequence in fragments shorter than    8222 bp
  55% of sequence in fragments shorter than    9136 bp
  60% of sequence in fragments shorter than   10155 bp
  65% of sequence in fragments shorter than   11313 bp
  70% of sequence in fragments shorter than   12624 bp
  75% of sequence in fragments shorter than   14164 bp
  80% of sequence in fragments shorter than   16076 bp
  85% of sequence in fragments shorter than   18549 bp
  90% of sequence in fragments shorter than   22214 bp
  95% of sequence in fragments shorter than   29364 bp

# ScaI.SnaBI (AGTACT+TACGTA) double digest, 675 thousand fragments (543793+132299 sites), 4410 bp average fragment length
   5% of sequence in fragments shorter than    1522 bp
  10% of sequence in fragments shorter than    2311 bp
  15% of sequence in fragments shorter than    2988 bp
  20% of sequence in fragments shorter than    3646 bp
  25% of sequence in fragments shorter than    4292 bp
  30% of sequence in fragments shorter than    4945 bp
  35% of sequence in fragments shorter than    5619 bp
  40% of sequence in fragments shorter than    6316 bp
  45% of sequence in fragments shorter than    7051 bp
  50% of sequence in fragments shorter than    7837 bp
  55% of sequence in fragments shorter than    8677 bp
  60% of sequence in fragments shorter than    9604 bp
  65% of sequence in fragments shorter than   10650 bp
  70% of sequence in fragments shorter than   11864 bp
  75% of sequence in fragments shorter than   13308 bp
  80% of sequence in fragments shorter than   15084 bp
  85% of sequence in fragments shorter than   17416 bp
  90% of sequence in fragments shorter than   21007 bp
  95% of sequence in fragments shorter than   28672 bp

# SapI.SexAI (GCTCTTC+ACCWGGT) double digest, 674 thousand fragments (377161+297900 sites), 4416 bp average fragment length
   5% of sequence in fragments shorter than    1511 bp
  10% of sequence in fragments shorter than    2321 bp
  15% of sequence in fragments shorter than    3026 bp
  20% of sequence in fragments shorter than    3699 bp
  25% of sequence in fragments shorter than    4352 bp
  30% of sequence in fragments shorter than    4994 bp
  35% of sequence in fragments shorter than    5661 bp
  40% of sequence in fragments shorter than    6344 bp
  45% of sequence in fragments shorter than    7064 bp
  50% of sequence in fragments shorter than    7828 bp
  55% of sequence in fragments shorter than    8645 bp
  60% of sequence in fragments shorter than    9553 bp
  65% of sequence in fragments shorter than   10577 bp
  70% of sequence in fragments shorter than   11745 bp
  75% of sequence in fragments shorter than   13095 bp
  80% of sequence in fragments shorter than   14760 bp
  85% of sequence in fragments shorter than   16932 bp
  90% of sequence in fragments shorter than   20223 bp
  95% of sequence in fragments shorter than   27071 bp

# SexAI.SmaI (ACCWGGT+CCCGGG) double digest, 674 thousand fragments (297900+376940 sites), 4417 bp average fragment length
   5% of sequence in fragments shorter than    1442 bp
  10% of sequence in fragments shorter than    2286 bp
  15% of sequence in fragments shorter than    3061 bp
  20% of sequence in fragments shorter than    3811 bp
  25% of sequence in fragments shorter than    4572 bp
  30% of sequence in fragments shorter than    5354 bp
  35% of sequence in fragments shorter than    6189 bp
  40% of sequence in fragments shorter than    7056 bp
  45% of sequence in fragments shorter than    7991 bp
  50% of sequence in fragments shorter than    8982 bp
  55% of sequence in fragments shorter than   10078 bp
  60% of sequence in fragments shorter than   11304 bp
  65% of sequence in fragments shorter than   12701 bp
  70% of sequence in fragments shorter than   14312 bp
  75% of sequence in fragments shorter than   16241 bp
  80% of sequence in fragments shorter than   18663 bp
  85% of sequence in fragments shorter than   21849 bp
  90% of sequence in fragments shorter than   26643 bp
  95% of sequence in fragments shorter than   37112 bp

# AflII.SalI (CTTAAG+GTCGAC) double digest, 674 thousand fragments (643284+31396 sites), 4419 bp average fragment length
   5% of sequence in fragments shorter than    1557 bp
  10% of sequence in fragments shorter than    2338 bp
  15% of sequence in fragments shorter than    3008 bp
  20% of sequence in fragments shorter than    3640 bp
  25% of sequence in fragments shorter than    4252 bp
  30% of sequence in fragments shorter than    4861 bp
  35% of sequence in fragments shorter than    5483 bp
  40% of sequence in fragments shorter than    6128 bp
  45% of sequence in fragments shorter than    6807 bp
  50% of sequence in fragments shorter than    7536 bp
  55% of sequence in fragments shorter than    8306 bp
  60% of sequence in fragments shorter than    9168 bp
  65% of sequence in fragments shorter than   10126 bp
  70% of sequence in fragments shorter than   11221 bp
  75% of sequence in fragments shorter than   12510 bp
  80% of sequence in fragments shorter than   14101 bp
  85% of sequence in fragments shorter than   16224 bp
  90% of sequence in fragments shorter than   19397 bp
  95% of sequence in fragments shorter than   26432 bp

# AatII.SacI (GACGTC+GAGCTC) double digest, 674 thousand fragments (71089+603323 sites), 4421 bp average fragment length
   5% of sequence in fragments shorter than    1497 bp
  10% of sequence in fragments shorter than    2303 bp
  15% of sequence in fragments shorter than    3026 bp
  20% of sequence in fragments shorter than    3719 bp
  25% of sequence in fragments shorter than    4400 bp
  30% of sequence in fragments shorter than    5075 bp
  35% of sequence in fragments shorter than    5784 bp
  40% of sequence in fragments shorter than    6517 bp
  45% of sequence in fragments shorter than    7290 bp
  50% of sequence in fragments shorter than    8120 bp
  55% of sequence in fragments shorter than    9013 bp
  60% of sequence in fragments shorter than   10025 bp
  65% of sequence in fragments shorter than   11147 bp
  70% of sequence in fragments shorter than   12396 bp
  75% of sequence in fragments shorter than   13902 bp
  80% of sequence in fragments shorter than   15731 bp
  85% of sequence in fragments shorter than   18159 bp
  90% of sequence in fragments shorter than   21727 bp
  95% of sequence in fragments shorter than   28804 bp

# EcoRV.SwaI (GATATC+ATTTAAAT) double digest, 672 thousand fragments (446473+226602 sites), 4430 bp average fragment length
   5% of sequence in fragments shorter than    1509 bp
  10% of sequence in fragments shorter than    2308 bp
  15% of sequence in fragments shorter than    3016 bp
  20% of sequence in fragments shorter than    3681 bp
  25% of sequence in fragments shorter than    4343 bp
  30% of sequence in fragments shorter than    5006 bp
  35% of sequence in fragments shorter than    5684 bp
  40% of sequence in fragments shorter than    6397 bp
  45% of sequence in fragments shorter than    7153 bp
  50% of sequence in fragments shorter than    7973 bp
  55% of sequence in fragments shorter than    8870 bp
  60% of sequence in fragments shorter than    9868 bp
  65% of sequence in fragments shorter than   11015 bp
  70% of sequence in fragments shorter than   12341 bp
  75% of sequence in fragments shorter than   13951 bp
  80% of sequence in fragments shorter than   16017 bp
  85% of sequence in fragments shorter than   18908 bp
  90% of sequence in fragments shorter than   23493 bp
  95% of sequence in fragments shorter than   33605 bp

# SphI.XhoI (GCATGC+CTCGAG) double digest, 671 thousand fragments (550952+121308 sites), 4435 bp average fragment length
   5% of sequence in fragments shorter than    1543 bp
  10% of sequence in fragments shorter than    2340 bp
  15% of sequence in fragments shorter than    3041 bp
  20% of sequence in fragments shorter than    3705 bp
  25% of sequence in fragments shorter than    4356 bp
  30% of sequence in fragments shorter than    5013 bp
  35% of sequence in fragments shorter than    5677 bp
  40% of sequence in fragments shorter than    6365 bp
  45% of sequence in fragments shorter than    7084 bp
  50% of sequence in fragments shorter than    7847 bp
  55% of sequence in fragments shorter than    8686 bp
  60% of sequence in fragments shorter than    9595 bp
  65% of sequence in fragments shorter than   10622 bp
  70% of sequence in fragments shorter than   11806 bp
  75% of sequence in fragments shorter than   13191 bp
  80% of sequence in fragments shorter than   14899 bp
  85% of sequence in fragments shorter than   17099 bp
  90% of sequence in fragments shorter than   20374 bp
  95% of sequence in fragments shorter than   26829 bp

# HaeII.PmeI (RGCGCY+GTTTAAAC) double digest, 671 thousand fragments (630101+42094 sites), 4436 bp average fragment length
   5% of sequence in fragments shorter than    1366 bp
  10% of sequence in fragments shorter than    2472 bp
  15% of sequence in fragments shorter than    3538 bp
  20% of sequence in fragments shorter than    4582 bp
  25% of sequence in fragments shorter than    5627 bp
  30% of sequence in fragments shorter than    6710 bp
  35% of sequence in fragments shorter than    7835 bp
  40% of sequence in fragments shorter than    9046 bp
  45% of sequence in fragments shorter than   10329 bp
  50% of sequence in fragments shorter than   11725 bp
  55% of sequence in fragments shorter than   13247 bp
  60% of sequence in fragments shorter than   14990 bp
  65% of sequence in fragments shorter than   16906 bp
  70% of sequence in fragments shorter than   19138 bp
  75% of sequence in fragments shorter than   21764 bp
  80% of sequence in fragments shorter than   24984 bp
  85% of sequence in fragments shorter than   29167 bp
  90% of sequence in fragments shorter than   34938 bp
  95% of sequence in fragments shorter than   45356 bp

# SacI.SacII (GAGCTC+CCGCGG) double digest, 669 thousand fragments (603323+66901 sites), 4448 bp average fragment length
   5% of sequence in fragments shorter than    1520 bp
  10% of sequence in fragments shorter than    2380 bp
  15% of sequence in fragments shorter than    3152 bp
  20% of sequence in fragments shorter than    3894 bp
  25% of sequence in fragments shorter than    4612 bp
  30% of sequence in fragments shorter than    5335 bp
  35% of sequence in fragments shorter than    6087 bp
  40% of sequence in fragments shorter than    6874 bp
  45% of sequence in fragments shorter than    7702 bp
  50% of sequence in fragments shorter than    8586 bp
  55% of sequence in fragments shorter than    9558 bp
  60% of sequence in fragments shorter than   10623 bp
  65% of sequence in fragments shorter than   11818 bp
  70% of sequence in fragments shorter than   13180 bp
  75% of sequence in fragments shorter than   14772 bp
  80% of sequence in fragments shorter than   16730 bp
  85% of sequence in fragments shorter than   19375 bp
  90% of sequence in fragments shorter than   23363 bp
  95% of sequence in fragments shorter than   31105 bp

# NaeI.ScaI (GCCGGC+AGTACT) double digest, 669 thousand fragments (125692+543793 sites), 4453 bp average fragment length
   5% of sequence in fragments shorter than    1568 bp
  10% of sequence in fragments shorter than    2386 bp
  15% of sequence in fragments shorter than    3089 bp
  20% of sequence in fragments shorter than    3764 bp
  25% of sequence in fragments shorter than    4420 bp
  30% of sequence in fragments shorter than    5074 bp
  35% of sequence in fragments shorter than    5751 bp
  40% of sequence in fragments shorter than    6445 bp
  45% of sequence in fragments shorter than    7175 bp
  50% of sequence in fragments shorter than    7938 bp
  55% of sequence in fragments shorter than    8770 bp
  60% of sequence in fragments shorter than    9664 bp
  65% of sequence in fragments shorter than   10672 bp
  70% of sequence in fragments shorter than   11820 bp
  75% of sequence in fragments shorter than   13145 bp
  80% of sequence in fragments shorter than   14796 bp
  85% of sequence in fragments shorter than   16885 bp
  90% of sequence in fragments shorter than   19996 bp
  95% of sequence in fragments shorter than   26189 bp

# AclI.ApaLI (AACGTT+GTGCAC) double digest, 668 thousand fragments (172706+496255 sites), 4457 bp average fragment length
   5% of sequence in fragments shorter than    1542 bp
  10% of sequence in fragments shorter than    2362 bp
  15% of sequence in fragments shorter than    3076 bp
  20% of sequence in fragments shorter than    3730 bp
  25% of sequence in fragments shorter than    4387 bp
  30% of sequence in fragments shorter than    5023 bp
  35% of sequence in fragments shorter than    5689 bp
  40% of sequence in fragments shorter than    6362 bp
  45% of sequence in fragments shorter than    7078 bp
  50% of sequence in fragments shorter than    7835 bp
  55% of sequence in fragments shorter than    8641 bp
  60% of sequence in fragments shorter than    9510 bp
  65% of sequence in fragments shorter than   10486 bp
  70% of sequence in fragments shorter than   11589 bp
  75% of sequence in fragments shorter than   12908 bp
  80% of sequence in fragments shorter than   14492 bp
  85% of sequence in fragments shorter than   16526 bp
  90% of sequence in fragments shorter than   19481 bp
  95% of sequence in fragments shorter than   24916 bp

# AatII.AvrII (GACGTC+CCTAGG) double digest, 665 thousand fragments (71089+594956 sites), 4476 bp average fragment length
   5% of sequence in fragments shorter than    1554 bp
  10% of sequence in fragments shorter than    2387 bp
  15% of sequence in fragments shorter than    3110 bp
  20% of sequence in fragments shorter than    3777 bp
  25% of sequence in fragments shorter than    4427 bp
  30% of sequence in fragments shorter than    5075 bp
  35% of sequence in fragments shorter than    5729 bp
  40% of sequence in fragments shorter than    6415 bp
  45% of sequence in fragments shorter than    7132 bp
  50% of sequence in fragments shorter than    7896 bp
  55% of sequence in fragments shorter than    8712 bp
  60% of sequence in fragments shorter than    9609 bp
  65% of sequence in fragments shorter than   10603 bp
  70% of sequence in fragments shorter than   11745 bp
  75% of sequence in fragments shorter than   13118 bp
  80% of sequence in fragments shorter than   14784 bp
  85% of sequence in fragments shorter than   16958 bp
  90% of sequence in fragments shorter than   20167 bp
  95% of sequence in fragments shorter than   26767 bp

# KpnI.SapI (GGTACC+GCTCTTC) double digest, 664 thousand fragments (287974+377161 sites), 4482 bp average fragment length
   5% of sequence in fragments shorter than    1460 bp
  10% of sequence in fragments shorter than    2304 bp
  15% of sequence in fragments shorter than    3037 bp
  20% of sequence in fragments shorter than    3734 bp
  25% of sequence in fragments shorter than    4419 bp
  30% of sequence in fragments shorter than    5110 bp
  35% of sequence in fragments shorter than    5805 bp
  40% of sequence in fragments shorter than    6523 bp
  45% of sequence in fragments shorter than    7275 bp
  50% of sequence in fragments shorter than    8065 bp
  55% of sequence in fragments shorter than    8930 bp
  60% of sequence in fragments shorter than    9869 bp
  65% of sequence in fragments shorter than   10924 bp
  70% of sequence in fragments shorter than   12148 bp
  75% of sequence in fragments shorter than   13551 bp
  80% of sequence in fragments shorter than   15271 bp
  85% of sequence in fragments shorter than   17544 bp
  90% of sequence in fragments shorter than   20959 bp
  95% of sequence in fragments shorter than   27906 bp

# ScaI.XhoI (AGTACT+CTCGAG) double digest, 664 thousand fragments (543793+121308 sites), 4483 bp average fragment length
   5% of sequence in fragments shorter than    1574 bp
  10% of sequence in fragments shorter than    2373 bp
  15% of sequence in fragments shorter than    3061 bp
  20% of sequence in fragments shorter than    3715 bp
  25% of sequence in fragments shorter than    4350 bp
  30% of sequence in fragments shorter than    4982 bp
  35% of sequence in fragments shorter than    5621 bp
  40% of sequence in fragments shorter than    6283 bp
  45% of sequence in fragments shorter than    6978 bp
  50% of sequence in fragments shorter than    7715 bp
  55% of sequence in fragments shorter than    8498 bp
  60% of sequence in fragments shorter than    9361 bp
  65% of sequence in fragments shorter than   10309 bp
  70% of sequence in fragments shorter than   11420 bp
  75% of sequence in fragments shorter than   12706 bp
  80% of sequence in fragments shorter than   14277 bp
  85% of sequence in fragments shorter than   16271 bp
  90% of sequence in fragments shorter than   19241 bp
  95% of sequence in fragments shorter than   25131 bp

# KpnI.SmaI (GGTACC+CCCGGG) double digest, 664 thousand fragments (287974+376940 sites), 4483 bp average fragment length
   5% of sequence in fragments shorter than    1468 bp
  10% of sequence in fragments shorter than    2291 bp
  15% of sequence in fragments shorter than    3075 bp
  20% of sequence in fragments shorter than    3821 bp
  25% of sequence in fragments shorter than    4579 bp
  30% of sequence in fragments shorter than    5350 bp
  35% of sequence in fragments shorter than    6159 bp
  40% of sequence in fragments shorter than    7009 bp
  45% of sequence in fragments shorter than    7912 bp
  50% of sequence in fragments shorter than    8884 bp
  55% of sequence in fragments shorter than    9920 bp
  60% of sequence in fragments shorter than   11063 bp
  65% of sequence in fragments shorter than   12376 bp
  70% of sequence in fragments shorter than   13903 bp
  75% of sequence in fragments shorter than   15696 bp
  80% of sequence in fragments shorter than   17922 bp
  85% of sequence in fragments shorter than   20844 bp
  90% of sequence in fragments shorter than   25307 bp
  95% of sequence in fragments shorter than   34761 bp

# AflII.MluI (CTTAAG+ACGCGT) double digest, 664 thousand fragments (643284+21415 sites), 4485 bp average fragment length
   5% of sequence in fragments shorter than    1582 bp
  10% of sequence in fragments shorter than    2374 bp
  15% of sequence in fragments shorter than    3054 bp
  20% of sequence in fragments shorter than    3697 bp
  25% of sequence in fragments shorter than    4324 bp
  30% of sequence in fragments shorter than    4942 bp
  35% of sequence in fragments shorter than    5573 bp
  40% of sequence in fragments shorter than    6227 bp
  45% of sequence in fragments shorter than    6915 bp
  50% of sequence in fragments shorter than    7649 bp
  55% of sequence in fragments shorter than    8434 bp
  60% of sequence in fragments shorter than    9295 bp
  65% of sequence in fragments shorter than   10263 bp
  70% of sequence in fragments shorter than   11374 bp
  75% of sequence in fragments shorter than   12674 bp
  80% of sequence in fragments shorter than   14264 bp
  85% of sequence in fragments shorter than   16370 bp
  90% of sequence in fragments shorter than   19532 bp
  95% of sequence in fragments shorter than   26480 bp

# NheI.SapI (GCTAGC+GCTCTTC) double digest, 664 thousand fragments (287384+377161 sites), 4486 bp average fragment length
   5% of sequence in fragments shorter than    1522 bp
  10% of sequence in fragments shorter than    2294 bp
  15% of sequence in fragments shorter than    3050 bp
  20% of sequence in fragments shorter than    3760 bp
  25% of sequence in fragments shorter than    4457 bp
  30% of sequence in fragments shorter than    5146 bp
  35% of sequence in fragments shorter than    5843 bp
  40% of sequence in fragments shorter than    6567 bp
  45% of sequence in fragments shorter than    7326 bp
  50% of sequence in fragments shorter than    8125 bp
  55% of sequence in fragments shorter than    8984 bp
  60% of sequence in fragments shorter than    9940 bp
  65% of sequence in fragments shorter than   10986 bp
  70% of sequence in fragments shorter than   12177 bp
  75% of sequence in fragments shorter than   13560 bp
  80% of sequence in fragments shorter than   15244 bp
  85% of sequence in fragments shorter than   17488 bp
  90% of sequence in fragments shorter than   20787 bp
  95% of sequence in fragments shorter than   27287 bp

# NheI.SmaI (GCTAGC+CCCGGG) double digest, 663 thousand fragments (287384+376940 sites), 4488 bp average fragment length
   5% of sequence in fragments shorter than    1547 bp
  10% of sequence in fragments shorter than    2320 bp
  15% of sequence in fragments shorter than    3049 bp
  20% of sequence in fragments shorter than    3811 bp
  25% of sequence in fragments shorter than    4575 bp
  30% of sequence in fragments shorter than    5341 bp
  35% of sequence in fragments shorter than    6144 bp
  40% of sequence in fragments shorter than    6990 bp
  45% of sequence in fragments shorter than    7875 bp
  50% of sequence in fragments shorter than    8828 bp
  55% of sequence in fragments shorter than    9864 bp
  60% of sequence in fragments shorter than   10996 bp
  65% of sequence in fragments shorter than   12273 bp
  70% of sequence in fragments shorter than   13762 bp
  75% of sequence in fragments shorter than   15494 bp
  80% of sequence in fragments shorter than   17638 bp
  85% of sequence in fragments shorter than   20458 bp
  90% of sequence in fragments shorter than   24617 bp
  95% of sequence in fragments shorter than   32917 bp

# BamHI.SexAI (GGATCC+ACCWGGT) double digest, 663 thousand fragments (365999+297900 sites), 4490 bp average fragment length
   5% of sequence in fragments shorter than    1556 bp
  10% of sequence in fragments shorter than    2350 bp
  15% of sequence in fragments shorter than    3066 bp
  20% of sequence in fragments shorter than    3740 bp
  25% of sequence in fragments shorter than    4403 bp
  30% of sequence in fragments shorter than    5052 bp
  35% of sequence in fragments shorter than    5726 bp
  40% of sequence in fragments shorter than    6434 bp
  45% of sequence in fragments shorter than    7173 bp
  50% of sequence in fragments shorter than    7959 bp
  55% of sequence in fragments shorter than    8813 bp
  60% of sequence in fragments shorter than    9752 bp
  65% of sequence in fragments shorter than   10809 bp
  70% of sequence in fragments shorter than   12002 bp
  75% of sequence in fragments shorter than   13392 bp
  80% of sequence in fragments shorter than   15117 bp
  85% of sequence in fragments shorter than   17363 bp
  90% of sequence in fragments shorter than   20625 bp
  95% of sequence in fragments shorter than   27176 bp

# PspXI.SacI (VCTCGAGB+GAGCTC) double digest, 662 thousand fragments (59193+603323 sites), 4500 bp average fragment length
   5% of sequence in fragments shorter than    1527 bp
  10% of sequence in fragments shorter than    2350 bp
  15% of sequence in fragments shorter than    3093 bp
  20% of sequence in fragments shorter than    3807 bp
  25% of sequence in fragments shorter than    4501 bp
  30% of sequence in fragments shorter than    5197 bp
  35% of sequence in fragments shorter than    5921 bp
  40% of sequence in fragments shorter than    6679 bp
  45% of sequence in fragments shorter than    7478 bp
  50% of sequence in fragments shorter than    8328 bp
  55% of sequence in fragments shorter than    9262 bp
  60% of sequence in fragments shorter than   10299 bp
  65% of sequence in fragments shorter than   11451 bp
  70% of sequence in fragments shorter than   12753 bp
  75% of sequence in fragments shorter than   14313 bp
  80% of sequence in fragments shorter than   16195 bp
  85% of sequence in fragments shorter than   18675 bp
  90% of sequence in fragments shorter than   22416 bp
  95% of sequence in fragments shorter than   29739 bp

# AvrII.SacII (CCTAGG+CCGCGG) double digest, 661 thousand fragments (594956+66901 sites), 4504 bp average fragment length
   5% of sequence in fragments shorter than    1574 bp
  10% of sequence in fragments shorter than    2460 bp
  15% of sequence in fragments shorter than    3223 bp
  20% of sequence in fragments shorter than    3933 bp
  25% of sequence in fragments shorter than    4617 bp
  30% of sequence in fragments shorter than    5302 bp
  35% of sequence in fragments shorter than    5996 bp
  40% of sequence in fragments shorter than    6718 bp
  45% of sequence in fragments shorter than    7486 bp
  50% of sequence in fragments shorter than    8287 bp
  55% of sequence in fragments shorter than    9157 bp
  60% of sequence in fragments shorter than   10108 bp
  65% of sequence in fragments shorter than   11185 bp
  70% of sequence in fragments shorter than   12401 bp
  75% of sequence in fragments shorter than   13869 bp
  80% of sequence in fragments shorter than   15642 bp
  85% of sequence in fragments shorter than   17932 bp
  90% of sequence in fragments shorter than   21437 bp
  95% of sequence in fragments shorter than   28683 bp

# HaeII.SalI (RGCGCY+GTCGAC) double digest, 661 thousand fragments (630101+31396 sites), 4508 bp average fragment length
   5% of sequence in fragments shorter than    1373 bp
  10% of sequence in fragments shorter than    2514 bp
  15% of sequence in fragments shorter than    3625 bp
  20% of sequence in fragments shorter than    4720 bp
  25% of sequence in fragments shorter than    5828 bp
  30% of sequence in fragments shorter than    6976 bp
  35% of sequence in fragments shorter than    8183 bp
  40% of sequence in fragments shorter than    9483 bp
  45% of sequence in fragments shorter than   10874 bp
  50% of sequence in fragments shorter than   12390 bp
  55% of sequence in fragments shorter than   14060 bp
  60% of sequence in fragments shorter than   15959 bp
  65% of sequence in fragments shorter than   18080 bp
  70% of sequence in fragments shorter than   20560 bp
  75% of sequence in fragments shorter than   23482 bp
  80% of sequence in fragments shorter than   26969 bp
  85% of sequence in fragments shorter than   31590 bp
  90% of sequence in fragments shorter than   37908 bp
  95% of sequence in fragments shorter than   49403 bp

# AflII.SgrAI (CTTAAG+CRCCGGYG) double digest, 658 thousand fragments (643284+15270 sites), 4527 bp average fragment length
   5% of sequence in fragments shorter than    1593 bp
  10% of sequence in fragments shorter than    2394 bp
  15% of sequence in fragments shorter than    3083 bp
  20% of sequence in fragments shorter than    3730 bp
  25% of sequence in fragments shorter than    4364 bp
  30% of sequence in fragments shorter than    4988 bp
  35% of sequence in fragments shorter than    5628 bp
  40% of sequence in fragments shorter than    6292 bp
  45% of sequence in fragments shorter than    6988 bp
  50% of sequence in fragments shorter than    7729 bp
  55% of sequence in fragments shorter than    8525 bp
  60% of sequence in fragments shorter than    9401 bp
  65% of sequence in fragments shorter than   10395 bp
  70% of sequence in fragments shorter than   11502 bp
  75% of sequence in fragments shorter than   12823 bp
  80% of sequence in fragments shorter than   14447 bp
  85% of sequence in fragments shorter than   16597 bp
  90% of sequence in fragments shorter than   19850 bp
  95% of sequence in fragments shorter than   26937 bp

# ClaI.HgaI (ATCGAT+GACGC) double digest, 657 thousand fragments (86477+571889 sites), 4529 bp average fragment length
   5% of sequence in fragments shorter than    1525 bp
  10% of sequence in fragments shorter than    2519 bp
  15% of sequence in fragments shorter than    3399 bp
  20% of sequence in fragments shorter than    4273 bp
  25% of sequence in fragments shorter than    5163 bp
  30% of sequence in fragments shorter than    6055 bp
  35% of sequence in fragments shorter than    6984 bp
  40% of sequence in fragments shorter than    7961 bp
  45% of sequence in fragments shorter than    9001 bp
  50% of sequence in fragments shorter than   10140 bp
  55% of sequence in fragments shorter than   11367 bp
  60% of sequence in fragments shorter than   12720 bp
  65% of sequence in fragments shorter than   14245 bp
  70% of sequence in fragments shorter than   15949 bp
  75% of sequence in fragments shorter than   17962 bp
  80% of sequence in fragments shorter than   20435 bp
  85% of sequence in fragments shorter than   23605 bp
  90% of sequence in fragments shorter than   28329 bp
  95% of sequence in fragments shorter than   37254 bp

# AflII.NruI (CTTAAG+TCGCGA) double digest, 657 thousand fragments (643284+14872 sites), 4530 bp average fragment length
   5% of sequence in fragments shorter than    1595 bp
  10% of sequence in fragments shorter than    2392 bp
  15% of sequence in fragments shorter than    3083 bp
  20% of sequence in fragments shorter than    3727 bp
  25% of sequence in fragments shorter than    4362 bp
  30% of sequence in fragments shorter than    4984 bp
  35% of sequence in fragments shorter than    5624 bp
  40% of sequence in fragments shorter than    6286 bp
  45% of sequence in fragments shorter than    6985 bp
  50% of sequence in fragments shorter than    7729 bp
  55% of sequence in fragments shorter than    8529 bp
  60% of sequence in fragments shorter than    9410 bp
  65% of sequence in fragments shorter than   10403 bp
  70% of sequence in fragments shorter than   11520 bp
  75% of sequence in fragments shorter than   12849 bp
  80% of sequence in fragments shorter than   14497 bp
  85% of sequence in fragments shorter than   16682 bp
  90% of sequence in fragments shorter than   19992 bp
  95% of sequence in fragments shorter than   27301 bp

# ClaI.EciI (ATCGAT+GGCGGA) double digest, 657 thousand fragments (86477+571273 sites), 4533 bp average fragment length
   5% of sequence in fragments shorter than    1493 bp
  10% of sequence in fragments shorter than    2415 bp
  15% of sequence in fragments shorter than    3263 bp
  20% of sequence in fragments shorter than    4103 bp
  25% of sequence in fragments shorter than    4978 bp
  30% of sequence in fragments shorter than    5878 bp
  35% of sequence in fragments shorter than    6840 bp
  40% of sequence in fragments shorter than    7861 bp
  45% of sequence in fragments shorter than    8964 bp
  50% of sequence in fragments shorter than   10188 bp
  55% of sequence in fragments shorter than   11535 bp
  60% of sequence in fragments shorter than   13051 bp
  65% of sequence in fragments shorter than   14778 bp
  70% of sequence in fragments shorter than   16765 bp
  75% of sequence in fragments shorter than   19174 bp
  80% of sequence in fragments shorter than   22107 bp
  85% of sequence in fragments shorter than   26021 bp
  90% of sequence in fragments shorter than   31886 bp
  95% of sequence in fragments shorter than   43807 bp

# AflII.PvuI (CTTAAG+CGATCG) double digest, 657 thousand fragments (643284+14186 sites), 4534 bp average fragment length
   5% of sequence in fragments shorter than    1600 bp
  10% of sequence in fragments shorter than    2391 bp
  15% of sequence in fragments shorter than    3079 bp
  20% of sequence in fragments shorter than    3726 bp
  25% of sequence in fragments shorter than    4361 bp
  30% of sequence in fragments shorter than    4985 bp
  35% of sequence in fragments shorter than    5627 bp
  40% of sequence in fragments shorter than    6293 bp
  45% of sequence in fragments shorter than    6993 bp
  50% of sequence in fragments shorter than    7738 bp
  55% of sequence in fragments shorter than    8539 bp
  60% of sequence in fragments shorter than    9416 bp
  65% of sequence in fragments shorter than   10411 bp
  70% of sequence in fragments shorter than   11532 bp
  75% of sequence in fragments shorter than   12864 bp
  80% of sequence in fragments shorter than   14521 bp
  85% of sequence in fragments shorter than   16700 bp
  90% of sequence in fragments shorter than   19989 bp
  95% of sequence in fragments shorter than   27118 bp

# AflII.FseI (CTTAAG+GGCCGGCC) double digest, 656 thousand fragments (643284+13662 sites), 4538 bp average fragment length
   5% of sequence in fragments shorter than    1598 bp
  10% of sequence in fragments shorter than    2400 bp
  15% of sequence in fragments shorter than    3091 bp
  20% of sequence in fragments shorter than    3738 bp
  25% of sequence in fragments shorter than    4375 bp
  30% of sequence in fragments shorter than    4998 bp
  35% of sequence in fragments shorter than    5639 bp
  40% of sequence in fragments shorter than    6302 bp
  45% of sequence in fragments shorter than    7000 bp
  50% of sequence in fragments shorter than    7740 bp
  55% of sequence in fragments shorter than    8537 bp
  60% of sequence in fragments shorter than    9415 bp
  65% of sequence in fragments shorter than   10404 bp
  70% of sequence in fragments shorter than   11510 bp
  75% of sequence in fragments shorter than   12827 bp
  80% of sequence in fragments shorter than   14447 bp
  85% of sequence in fragments shorter than   16569 bp
  90% of sequence in fragments shorter than   19771 bp
  95% of sequence in fragments shorter than   26772 bp

# AgeI.SacI (ACCGGT+GAGCTC) double digest, 656 thousand fragments (53386+603323 sites), 4540 bp average fragment length
   5% of sequence in fragments shorter than    1547 bp
  10% of sequence in fragments shorter than    2378 bp
  15% of sequence in fragments shorter than    3124 bp
  20% of sequence in fragments shorter than    3836 bp
  25% of sequence in fragments shorter than    4531 bp
  30% of sequence in fragments shorter than    5227 bp
  35% of sequence in fragments shorter than    5946 bp
  40% of sequence in fragments shorter than    6695 bp
  45% of sequence in fragments shorter than    7490 bp
  50% of sequence in fragments shorter than    8328 bp
  55% of sequence in fragments shorter than    9245 bp
  60% of sequence in fragments shorter than   10271 bp
  65% of sequence in fragments shorter than   11403 bp
  70% of sequence in fragments shorter than   12703 bp
  75% of sequence in fragments shorter than   14239 bp
  80% of sequence in fragments shorter than   16095 bp
  85% of sequence in fragments shorter than   18517 bp
  90% of sequence in fragments shorter than   22260 bp
  95% of sequence in fragments shorter than   29573 bp

# ApaLI.PacI (GTGCAC+TTAATTAA) double digest, 655 thousand fragments (496255+159455 sites), 4547 bp average fragment length
   5% of sequence in fragments shorter than    1645 bp
  10% of sequence in fragments shorter than    2482 bp
  15% of sequence in fragments shorter than    3206 bp
  20% of sequence in fragments shorter than    3882 bp
  25% of sequence in fragments shorter than    4541 bp
  30% of sequence in fragments shorter than    5198 bp
  35% of sequence in fragments shorter than    5870 bp
  40% of sequence in fragments shorter than    6527 bp
  45% of sequence in fragments shorter than    7231 bp
  50% of sequence in fragments shorter than    7986 bp
  55% of sequence in fragments shorter than    8793 bp
  60% of sequence in fragments shorter than    9672 bp
  65% of sequence in fragments shorter than   10657 bp
  70% of sequence in fragments shorter than   11787 bp
  75% of sequence in fragments shorter than   13111 bp
  80% of sequence in fragments shorter than   14730 bp
  85% of sequence in fragments shorter than   16796 bp
  90% of sequence in fragments shorter than   19829 bp
  95% of sequence in fragments shorter than   25790 bp

# AvrII.PspXI (CCTAGG+VCTCGAGB) double digest, 653 thousand fragments (594956+59193 sites), 4558 bp average fragment length
   5% of sequence in fragments shorter than    1582 bp
  10% of sequence in fragments shorter than    2432 bp
  15% of sequence in fragments shorter than    3165 bp
  20% of sequence in fragments shorter than    3849 bp
  25% of sequence in fragments shorter than    4506 bp
  30% of sequence in fragments shorter than    5163 bp
  35% of sequence in fragments shorter than    5830 bp
  40% of sequence in fragments shorter than    6528 bp
  45% of sequence in fragments shorter than    7261 bp
  50% of sequence in fragments shorter than    8030 bp
  55% of sequence in fragments shorter than    8865 bp
  60% of sequence in fragments shorter than    9793 bp
  65% of sequence in fragments shorter than   10818 bp
  70% of sequence in fragments shorter than   11990 bp
  75% of sequence in fragments shorter than   13417 bp
  80% of sequence in fragments shorter than   15121 bp
  85% of sequence in fragments shorter than   17341 bp
  90% of sequence in fragments shorter than   20686 bp
  95% of sequence in fragments shorter than   27445 bp

# ApaLI.Hpy99I (GTGCAC+CGWCG) double digest, 653 thousand fragments (496255+157917 sites), 4558 bp average fragment length
   5% of sequence in fragments shorter than    1605 bp
  10% of sequence in fragments shorter than    2497 bp
  15% of sequence in fragments shorter than    3277 bp
  20% of sequence in fragments shorter than    4025 bp
  25% of sequence in fragments shorter than    4762 bp
  30% of sequence in fragments shorter than    5505 bp
  35% of sequence in fragments shorter than    6202 bp
  40% of sequence in fragments shorter than    6993 bp
  45% of sequence in fragments shorter than    7839 bp
  50% of sequence in fragments shorter than    8731 bp
  55% of sequence in fragments shorter than    9685 bp
  60% of sequence in fragments shorter than   10750 bp
  65% of sequence in fragments shorter than   11942 bp
  70% of sequence in fragments shorter than   13325 bp
  75% of sequence in fragments shorter than   14934 bp
  80% of sequence in fragments shorter than   16909 bp
  85% of sequence in fragments shorter than   19482 bp
  90% of sequence in fragments shorter than   23205 bp
  95% of sequence in fragments shorter than   30242 bp

# BamHI.KpnI (GGATCC+GGTACC) double digest, 653 thousand fragments (365999+287974 sites), 4559 bp average fragment length
   5% of sequence in fragments shorter than    1557 bp
  10% of sequence in fragments shorter than    2353 bp
  15% of sequence in fragments shorter than    3102 bp
  20% of sequence in fragments shorter than    3804 bp
  25% of sequence in fragments shorter than    4505 bp
  30% of sequence in fragments shorter than    5206 bp
  35% of sequence in fragments shorter than    5929 bp
  40% of sequence in fragments shorter than    6664 bp
  45% of sequence in fragments shorter than    7448 bp
  50% of sequence in fragments shorter than    8273 bp
  55% of sequence in fragments shorter than    9171 bp
  60% of sequence in fragments shorter than   10140 bp
  65% of sequence in fragments shorter than   11235 bp
  70% of sequence in fragments shorter than   12479 bp
  75% of sequence in fragments shorter than   13937 bp
  80% of sequence in fragments shorter than   15702 bp
  85% of sequence in fragments shorter than   18027 bp
  90% of sequence in fragments shorter than   21508 bp
  95% of sequence in fragments shorter than   28292 bp

# BamHI.NheI (GGATCC+GCTAGC) double digest, 653 thousand fragments (365999+287384 sites), 4563 bp average fragment length
   5% of sequence in fragments shorter than    1678 bp
  10% of sequence in fragments shorter than    2409 bp
  15% of sequence in fragments shorter than    3103 bp
  20% of sequence in fragments shorter than    3823 bp
  25% of sequence in fragments shorter than    4533 bp
  30% of sequence in fragments shorter than    5233 bp
  35% of sequence in fragments shorter than    5952 bp
  40% of sequence in fragments shorter than    6687 bp
  45% of sequence in fragments shorter than    7457 bp
  50% of sequence in fragments shorter than    8282 bp
  55% of sequence in fragments shorter than    9168 bp
  60% of sequence in fragments shorter than   10128 bp
  65% of sequence in fragments shorter than   11195 bp
  70% of sequence in fragments shorter than   12419 bp
  75% of sequence in fragments shorter than   13846 bp
  80% of sequence in fragments shorter than   15575 bp
  85% of sequence in fragments shorter than   17824 bp
  90% of sequence in fragments shorter than   21089 bp
  95% of sequence in fragments shorter than   27453 bp

# AflII.NotI (CTTAAG+GCGGCCGC) double digest, 652 thousand fragments (643284+9795 sites), 4565 bp average fragment length
   5% of sequence in fragments shorter than    1608 bp
  10% of sequence in fragments shorter than    2413 bp
  15% of sequence in fragments shorter than    3108 bp
  20% of sequence in fragments shorter than    3759 bp
  25% of sequence in fragments shorter than    4401 bp
  30% of sequence in fragments shorter than    5027 bp
  35% of sequence in fragments shorter than    5674 bp
  40% of sequence in fragments shorter than    6345 bp
  45% of sequence in fragments shorter than    7049 bp
  50% of sequence in fragments shorter than    7800 bp
  55% of sequence in fragments shorter than    8605 bp
  60% of sequence in fragments shorter than    9488 bp
  65% of sequence in fragments shorter than   10487 bp
  70% of sequence in fragments shorter than   11620 bp
  75% of sequence in fragments shorter than   12948 bp
  80% of sequence in fragments shorter than   14607 bp
  85% of sequence in fragments shorter than   16812 bp
  90% of sequence in fragments shorter than   20148 bp
  95% of sequence in fragments shorter than   27528 bp

# AflII.RsrII (CTTAAG+CGGWCCG) double digest, 652 thousand fragments (643284+9764 sites), 4565 bp average fragment length
   5% of sequence in fragments shorter than    1608 bp
  10% of sequence in fragments shorter than    2412 bp
  15% of sequence in fragments shorter than    3107 bp
  20% of sequence in fragments shorter than    3757 bp
  25% of sequence in fragments shorter than    4395 bp
  30% of sequence in fragments shorter than    5021 bp
  35% of sequence in fragments shorter than    5665 bp
  40% of sequence in fragments shorter than    6336 bp
  45% of sequence in fragments shorter than    7038 bp
  50% of sequence in fragments shorter than    7790 bp
  55% of sequence in fragments shorter than    8596 bp
  60% of sequence in fragments shorter than    9478 bp
  65% of sequence in fragments shorter than   10478 bp
  70% of sequence in fragments shorter than   11602 bp
  75% of sequence in fragments shorter than   12935 bp
  80% of sequence in fragments shorter than   14600 bp
  85% of sequence in fragments shorter than   16811 bp
  90% of sequence in fragments shorter than   20126 bp
  95% of sequence in fragments shorter than   27422 bp

# HaeII.MluI (RGCGCY+ACGCGT) double digest, 651 thousand fragments (630101+21415 sites), 4577 bp average fragment length
   5% of sequence in fragments shorter than    1379 bp
  10% of sequence in fragments shorter than    2562 bp
  15% of sequence in fragments shorter than    3707 bp
  20% of sequence in fragments shorter than    4838 bp
  25% of sequence in fragments shorter than    5995 bp
  30% of sequence in fragments shorter than    7197 bp
  35% of sequence in fragments shorter than    8485 bp
  40% of sequence in fragments shorter than    9859 bp
  45% of sequence in fragments shorter than   11322 bp
  50% of sequence in fragments shorter than   12950 bp
  55% of sequence in fragments shorter than   14757 bp
  60% of sequence in fragments shorter than   16753 bp
  65% of sequence in fragments shorter than   19051 bp
  70% of sequence in fragments shorter than   21681 bp
  75% of sequence in fragments shorter than   24779 bp
  80% of sequence in fragments shorter than   28532 bp
  85% of sequence in fragments shorter than   33440 bp
  90% of sequence in fragments shorter than   40371 bp
  95% of sequence in fragments shorter than   52692 bp

# ClaI.MfeI (ATCGAT+CAATTG) double digest, 650 thousand fragments (86477+564303 sites), 4582 bp average fragment length
   5% of sequence in fragments shorter than    1581 bp
  10% of sequence in fragments shorter than    2384 bp
  15% of sequence in fragments shorter than    3101 bp
  20% of sequence in fragments shorter than    3782 bp
  25% of sequence in fragments shorter than    4441 bp
  30% of sequence in fragments shorter than    5099 bp
  35% of sequence in fragments shorter than    5775 bp
  40% of sequence in fragments shorter than    6471 bp
  45% of sequence in fragments shorter than    7215 bp
  50% of sequence in fragments shorter than    8015 bp
  55% of sequence in fragments shorter than    8884 bp
  60% of sequence in fragments shorter than    9851 bp
  65% of sequence in fragments shorter than   10930 bp
  70% of sequence in fragments shorter than   12175 bp
  75% of sequence in fragments shorter than   13661 bp
  80% of sequence in fragments shorter than   15518 bp
  85% of sequence in fragments shorter than   18018 bp
  90% of sequence in fragments shorter than   21849 bp
  95% of sequence in fragments shorter than   29681 bp

# AgeI.AvrII (ACCGGT+CCTAGG) double digest, 647 thousand fragments (53386+594956 sites), 4598 bp average fragment length
   5% of sequence in fragments shorter than    1605 bp
  10% of sequence in fragments shorter than    2438 bp
  15% of sequence in fragments shorter than    3163 bp
  20% of sequence in fragments shorter than    3855 bp
  25% of sequence in fragments shorter than    4519 bp
  30% of sequence in fragments shorter than    5189 bp
  35% of sequence in fragments shorter than    5865 bp
  40% of sequence in fragments shorter than    6574 bp
  45% of sequence in fragments shorter than    7311 bp
  50% of sequence in fragments shorter than    8085 bp
  55% of sequence in fragments shorter than    8919 bp
  60% of sequence in fragments shorter than    9837 bp
  65% of sequence in fragments shorter than   10863 bp
  70% of sequence in fragments shorter than   12028 bp
  75% of sequence in fragments shorter than   13442 bp
  80% of sequence in fragments shorter than   15159 bp
  85% of sequence in fragments shorter than   17394 bp
  90% of sequence in fragments shorter than   20695 bp
  95% of sequence in fragments shorter than   27573 bp

# AflII.AscI (CTTAAG+GGCGCGCC) double digest, 647 thousand fragments (643284+4751 sites), 4600 bp average fragment length
   5% of sequence in fragments shorter than    1620 bp
  10% of sequence in fragments shorter than    2428 bp
  15% of sequence in fragments shorter than    3128 bp
  20% of sequence in fragments shorter than    3784 bp
  25% of sequence in fragments shorter than    4427 bp
  30% of sequence in fragments shorter than    5060 bp
  35% of sequence in fragments shorter than    5709 bp
  40% of sequence in fragments shorter than    6386 bp
  45% of sequence in fragments shorter than    7098 bp
  50% of sequence in fragments shorter than    7859 bp
  55% of sequence in fragments shorter than    8672 bp
  60% of sequence in fragments shorter than    9571 bp
  65% of sequence in fragments shorter than   10581 bp
  70% of sequence in fragments shorter than   11735 bp
  75% of sequence in fragments shorter than   13088 bp
  80% of sequence in fragments shorter than   14784 bp
  85% of sequence in fragments shorter than   17035 bp
  90% of sequence in fragments shorter than   20479 bp
  95% of sequence in fragments shorter than   28078 bp

# PmeI.SacI (GTTTAAAC+GAGCTC) double digest, 645 thousand fragments (42094+603323 sites), 4619 bp average fragment length
   5% of sequence in fragments shorter than    1579 bp
  10% of sequence in fragments shorter than    2414 bp
  15% of sequence in fragments shorter than    3162 bp
  20% of sequence in fragments shorter than    3872 bp
  25% of sequence in fragments shorter than    4560 bp
  30% of sequence in fragments shorter than    5253 bp
  35% of sequence in fragments shorter than    5964 bp
  40% of sequence in fragments shorter than    6707 bp
  45% of sequence in fragments shorter than    7488 bp
  50% of sequence in fragments shorter than    8311 bp
  55% of sequence in fragments shorter than    9209 bp
  60% of sequence in fragments shorter than   10209 bp
  65% of sequence in fragments shorter than   11330 bp
  70% of sequence in fragments shorter than   12570 bp
  75% of sequence in fragments shorter than   14057 bp
  80% of sequence in fragments shorter than   15871 bp
  85% of sequence in fragments shorter than   18243 bp
  90% of sequence in fragments shorter than   21765 bp
  95% of sequence in fragments shorter than   28660 bp

# HaeII.SgrAI (RGCGCY+CRCCGGYG) double digest, 644 thousand fragments (630101+15270 sites), 4620 bp average fragment length
   5% of sequence in fragments shorter than    1393 bp
  10% of sequence in fragments shorter than    2618 bp
  15% of sequence in fragments shorter than    3793 bp
  20% of sequence in fragments shorter than    4947 bp
  25% of sequence in fragments shorter than    6134 bp
  30% of sequence in fragments shorter than    7360 bp
  35% of sequence in fragments shorter than    8669 bp
  40% of sequence in fragments shorter than   10069 bp
  45% of sequence in fragments shorter than   11571 bp
  50% of sequence in fragments shorter than   13222 bp
  55% of sequence in fragments shorter than   15073 bp
  60% of sequence in fragments shorter than   17112 bp
  65% of sequence in fragments shorter than   19466 bp
  70% of sequence in fragments shorter than   22188 bp
  75% of sequence in fragments shorter than   25272 bp
  80% of sequence in fragments shorter than   29170 bp
  85% of sequence in fragments shorter than   34152 bp
  90% of sequence in fragments shorter than   41335 bp
  95% of sequence in fragments shorter than   53948 bp

# HaeII.NruI (RGCGCY+TCGCGA) double digest, 644 thousand fragments (630101+14872 sites), 4623 bp average fragment length
   5% of sequence in fragments shorter than    1387 bp
  10% of sequence in fragments shorter than    2599 bp
  15% of sequence in fragments shorter than    3762 bp
  20% of sequence in fragments shorter than    4910 bp
  25% of sequence in fragments shorter than    6075 bp
  30% of sequence in fragments shorter than    7294 bp
  35% of sequence in fragments shorter than    8588 bp
  40% of sequence in fragments shorter than    9970 bp
  45% of sequence in fragments shorter than   11468 bp
  50% of sequence in fragments shorter than   13084 bp
  55% of sequence in fragments shorter than   14922 bp
  60% of sequence in fragments shorter than   16930 bp
  65% of sequence in fragments shorter than   19260 bp
  70% of sequence in fragments shorter than   21952 bp
  75% of sequence in fragments shorter than   25036 bp
  80% of sequence in fragments shorter than   28882 bp
  85% of sequence in fragments shorter than   33802 bp
  90% of sequence in fragments shorter than   40896 bp
  95% of sequence in fragments shorter than   53396 bp

# HaeII.PvuI (RGCGCY+CGATCG) double digest, 643 thousand fragments (630101+14186 sites), 4628 bp average fragment length
   5% of sequence in fragments shorter than    1387 bp
  10% of sequence in fragments shorter than    2600 bp
  15% of sequence in fragments shorter than    3764 bp
  20% of sequence in fragments shorter than    4911 bp
  25% of sequence in fragments shorter than    6081 bp
  30% of sequence in fragments shorter than    7298 bp
  35% of sequence in fragments shorter than    8596 bp
  40% of sequence in fragments shorter than    9977 bp
  45% of sequence in fragments shorter than   11464 bp
  50% of sequence in fragments shorter than   13084 bp
  55% of sequence in fragments shorter than   14922 bp
  60% of sequence in fragments shorter than   16944 bp
  65% of sequence in fragments shorter than   19255 bp
  70% of sequence in fragments shorter than   21967 bp
  75% of sequence in fragments shorter than   25061 bp
  80% of sequence in fragments shorter than   28918 bp
  85% of sequence in fragments shorter than   33848 bp
  90% of sequence in fragments shorter than   40856 bp
  95% of sequence in fragments shorter than   53372 bp

# FseI.HaeII (GGCCGGCC+RGCGCY) double digest, 643 thousand fragments (13662+630101 sites), 4632 bp average fragment length
   5% of sequence in fragments shorter than    1387 bp
  10% of sequence in fragments shorter than    2607 bp
  15% of sequence in fragments shorter than    3785 bp
  20% of sequence in fragments shorter than    4946 bp
  25% of sequence in fragments shorter than    6135 bp
  30% of sequence in fragments shorter than    7369 bp
  35% of sequence in fragments shorter than    8683 bp
  40% of sequence in fragments shorter than   10088 bp
  45% of sequence in fragments shorter than   11600 bp
  50% of sequence in fragments shorter than   13264 bp
  55% of sequence in fragments shorter than   15114 bp
  60% of sequence in fragments shorter than   17171 bp
  65% of sequence in fragments shorter than   19531 bp
  70% of sequence in fragments shorter than   22270 bp
  75% of sequence in fragments shorter than   25401 bp
  80% of sequence in fragments shorter than   29343 bp
  85% of sequence in fragments shorter than   34392 bp
  90% of sequence in fragments shorter than   41599 bp
  95% of sequence in fragments shorter than   54433 bp

# AatII.HgaI (GACGTC+GACGC) double digest, 642 thousand fragments (71089+571889 sites), 4637 bp average fragment length
   5% of sequence in fragments shorter than    1542 bp
  10% of sequence in fragments shorter than    2593 bp
  15% of sequence in fragments shorter than    3529 bp
  20% of sequence in fragments shorter than    4488 bp
  25% of sequence in fragments shorter than    5456 bp
  30% of sequence in fragments shorter than    6448 bp
  35% of sequence in fragments shorter than    7471 bp
  40% of sequence in fragments shorter than    8563 bp
  45% of sequence in fragments shorter than    9739 bp
  50% of sequence in fragments shorter than   11005 bp
  55% of sequence in fragments shorter than   12400 bp
  60% of sequence in fragments shorter than   13948 bp
  65% of sequence in fragments shorter than   15673 bp
  70% of sequence in fragments shorter than   17650 bp
  75% of sequence in fragments shorter than   20015 bp
  80% of sequence in fragments shorter than   22879 bp
  85% of sequence in fragments shorter than   26693 bp
  90% of sequence in fragments shorter than   32311 bp
  95% of sequence in fragments shorter than   42990 bp

# AatII.EciI (GACGTC+GGCGGA) double digest, 642 thousand fragments (71089+571273 sites), 4641 bp average fragment length
   5% of sequence in fragments shorter than    1496 bp
  10% of sequence in fragments shorter than    2456 bp
  15% of sequence in fragments shorter than    3349 bp
  20% of sequence in fragments shorter than    4258 bp
  25% of sequence in fragments shorter than    5205 bp
  30% of sequence in fragments shorter than    6204 bp
  35% of sequence in fragments shorter than    7272 bp
  40% of sequence in fragments shorter than    8428 bp
  45% of sequence in fragments shorter than    9718 bp
  50% of sequence in fragments shorter than   11125 bp
  55% of sequence in fragments shorter than   12720 bp
  60% of sequence in fragments shorter than   14518 bp
  65% of sequence in fragments shorter than   16583 bp
  70% of sequence in fragments shorter than   19015 bp
  75% of sequence in fragments shorter than   21872 bp
  80% of sequence in fragments shorter than   25515 bp
  85% of sequence in fragments shorter than   30249 bp
  90% of sequence in fragments shorter than   37523 bp
  95% of sequence in fragments shorter than   51687 bp

# HaeII.NotI (RGCGCY+GCGGCCGC) double digest, 639 thousand fragments (630101+9795 sites), 4660 bp average fragment length
   5% of sequence in fragments shorter than    1406 bp
  10% of sequence in fragments shorter than    2643 bp
  15% of sequence in fragments shorter than    3830 bp
  20% of sequence in fragments shorter than    4998 bp
  25% of sequence in fragments shorter than    6192 bp
  30% of sequence in fragments shorter than    7434 bp
  35% of sequence in fragments shorter than    8756 bp
  40% of sequence in fragments shorter than   10176 bp
  45% of sequence in fragments shorter than   11702 bp
  50% of sequence in fragments shorter than   13368 bp
  55% of sequence in fragments shorter than   15239 bp
  60% of sequence in fragments shorter than   17302 bp
  65% of sequence in fragments shorter than   19677 bp
  70% of sequence in fragments shorter than   22430 bp
  75% of sequence in fragments shorter than   25576 bp
  80% of sequence in fragments shorter than   29527 bp
  85% of sequence in fragments shorter than   34574 bp
  90% of sequence in fragments shorter than   41834 bp
  95% of sequence in fragments shorter than   54701 bp

# HaeII.RsrII (RGCGCY+CGGWCCG) double digest, 639 thousand fragments (630101+9764 sites), 4660 bp average fragment length
   5% of sequence in fragments shorter than    1398 bp
  10% of sequence in fragments shorter than    2628 bp
  15% of sequence in fragments shorter than    3810 bp
  20% of sequence in fragments shorter than    4973 bp
  25% of sequence in fragments shorter than    6161 bp
  30% of sequence in fragments shorter than    7396 bp
  35% of sequence in fragments shorter than    8711 bp
  40% of sequence in fragments shorter than   10119 bp
  45% of sequence in fragments shorter than   11630 bp
  50% of sequence in fragments shorter than   13299 bp
  55% of sequence in fragments shorter than   15154 bp
  60% of sequence in fragments shorter than   17204 bp
  65% of sequence in fragments shorter than   19569 bp
  70% of sequence in fragments shorter than   22320 bp
  75% of sequence in fragments shorter than   25424 bp
  80% of sequence in fragments shorter than   29358 bp
  85% of sequence in fragments shorter than   34415 bp
  90% of sequence in fragments shorter than   41610 bp
  95% of sequence in fragments shorter than   54364 bp

# HgaI.SacII (GACGC+CCGCGG) double digest, 638 thousand fragments (571889+66901 sites), 4667 bp average fragment length
   5% of sequence in fragments shorter than    1626 bp
  10% of sequence in fragments shorter than    2770 bp
  15% of sequence in fragments shorter than    3829 bp
  20% of sequence in fragments shorter than    4893 bp
  25% of sequence in fragments shorter than    5964 bp
  30% of sequence in fragments shorter than    7058 bp
  35% of sequence in fragments shorter than    8211 bp
  40% of sequence in fragments shorter than    9436 bp
  45% of sequence in fragments shorter than   10755 bp
  50% of sequence in fragments shorter than   12182 bp
  55% of sequence in fragments shorter than   13768 bp
  60% of sequence in fragments shorter than   15509 bp
  65% of sequence in fragments shorter than   17432 bp
  70% of sequence in fragments shorter than   19700 bp
  75% of sequence in fragments shorter than   22352 bp
  80% of sequence in fragments shorter than   25609 bp
  85% of sequence in fragments shorter than   29890 bp
  90% of sequence in fragments shorter than   36155 bp
  95% of sequence in fragments shorter than   48336 bp

# EciI.SacII (GGCGGA+CCGCGG) double digest, 637 thousand fragments (571273+66901 sites), 4671 bp average fragment length
   5% of sequence in fragments shorter than    1537 bp
  10% of sequence in fragments shorter than    2578 bp
  15% of sequence in fragments shorter than    3558 bp
  20% of sequence in fragments shorter than    4565 bp
  25% of sequence in fragments shorter than    5628 bp
  30% of sequence in fragments shorter than    6760 bp
  35% of sequence in fragments shorter than    7984 bp
  40% of sequence in fragments shorter than    9317 bp
  45% of sequence in fragments shorter than   10805 bp
  50% of sequence in fragments shorter than   12454 bp
  55% of sequence in fragments shorter than   14295 bp
  60% of sequence in fragments shorter than   16406 bp
  65% of sequence in fragments shorter than   18818 bp
  70% of sequence in fragments shorter than   21607 bp
  75% of sequence in fragments shorter than   25023 bp
  80% of sequence in fragments shorter than   29218 bp
  85% of sequence in fragments shorter than   34936 bp
  90% of sequence in fragments shorter than   43501 bp
  95% of sequence in fragments shorter than   61533 bp

# ClaI.SphI (ATCGAT+GCATGC) double digest, 637 thousand fragments (86477+550952 sites), 4677 bp average fragment length
   5% of sequence in fragments shorter than    1645 bp
  10% of sequence in fragments shorter than    2469 bp
  15% of sequence in fragments shorter than    3197 bp
  20% of sequence in fragments shorter than    3884 bp
  25% of sequence in fragments shorter than    4558 bp
  30% of sequence in fragments shorter than    5229 bp
  35% of sequence in fragments shorter than    5909 bp
  40% of sequence in fragments shorter than    6615 bp
  45% of sequence in fragments shorter than    7351 bp
  50% of sequence in fragments shorter than    8133 bp
  55% of sequence in fragments shorter than    8969 bp
  60% of sequence in fragments shorter than    9896 bp
  65% of sequence in fragments shorter than   10946 bp
  70% of sequence in fragments shorter than   12119 bp
  75% of sequence in fragments shorter than   13505 bp
  80% of sequence in fragments shorter than   15206 bp
  85% of sequence in fragments shorter than   17386 bp
  90% of sequence in fragments shorter than   20588 bp
  95% of sequence in fragments shorter than   26832 bp

# AvrII.PmeI (CCTAGG+GTTTAAAC) double digest, 636 thousand fragments (594956+42094 sites), 4680 bp average fragment length
   5% of sequence in fragments shorter than    1638 bp
  10% of sequence in fragments shorter than    2505 bp
  15% of sequence in fragments shorter than    3251 bp
  20% of sequence in fragments shorter than    3948 bp
  25% of sequence in fragments shorter than    4614 bp
  30% of sequence in fragments shorter than    5279 bp
  35% of sequence in fragments shorter than    5953 bp
  40% of sequence in fragments shorter than    6653 bp
  45% of sequence in fragments shorter than    7391 bp
  50% of sequence in fragments shorter than    8163 bp
  55% of sequence in fragments shorter than    8993 bp
  60% of sequence in fragments shorter than    9905 bp
  65% of sequence in fragments shorter than   10917 bp
  70% of sequence in fragments shorter than   12071 bp
  75% of sequence in fragments shorter than   13463 bp
  80% of sequence in fragments shorter than   15138 bp
  85% of sequence in fragments shorter than   17317 bp
  90% of sequence in fragments shorter than   20555 bp
  95% of sequence in fragments shorter than   27159 bp

# BsrBI.SpeI (CCGCTC+ACTAGT) double digest, 635 thousand fragments (235523+400286 sites), 4690 bp average fragment length
   5% of sequence in fragments shorter than    1658 bp
  10% of sequence in fragments shorter than    2530 bp
  15% of sequence in fragments shorter than    3264 bp
  20% of sequence in fragments shorter than    3989 bp
  25% of sequence in fragments shorter than    4699 bp
  30% of sequence in fragments shorter than    5398 bp
  35% of sequence in fragments shorter than    6116 bp
  40% of sequence in fragments shorter than    6853 bp
  45% of sequence in fragments shorter than    7615 bp
  50% of sequence in fragments shorter than    8433 bp
  55% of sequence in fragments shorter than    9305 bp
  60% of sequence in fragments shorter than   10264 bp
  65% of sequence in fragments shorter than   11317 bp
  70% of sequence in fragments shorter than   12483 bp
  75% of sequence in fragments shorter than   13844 bp
  80% of sequence in fragments shorter than   15523 bp
  85% of sequence in fragments shorter than   17656 bp
  90% of sequence in fragments shorter than   20748 bp
  95% of sequence in fragments shorter than   26367 bp

# AatII.MfeI (GACGTC+CAATTG) double digest, 634 thousand fragments (71089+564303 sites), 4693 bp average fragment length
   5% of sequence in fragments shorter than    1644 bp
  10% of sequence in fragments shorter than    2467 bp
  15% of sequence in fragments shorter than    3194 bp
  20% of sequence in fragments shorter than    3883 bp
  25% of sequence in fragments shorter than    4545 bp
  30% of sequence in fragments shorter than    5206 bp
  35% of sequence in fragments shorter than    5877 bp
  40% of sequence in fragments shorter than    6577 bp
  45% of sequence in fragments shorter than    7315 bp
  50% of sequence in fragments shorter than    8101 bp
  55% of sequence in fragments shorter than    8947 bp
  60% of sequence in fragments shorter than    9894 bp
  65% of sequence in fragments shorter than   10935 bp
  70% of sequence in fragments shorter than   12086 bp
  75% of sequence in fragments shorter than   13471 bp
  80% of sequence in fragments shorter than   15155 bp
  85% of sequence in fragments shorter than   17367 bp
  90% of sequence in fragments shorter than   20668 bp
  95% of sequence in fragments shorter than   27131 bp

# AscI.HaeII (GGCGCGCC+RGCGCY) double digest, 634 thousand fragments (4751+630101 sites), 4697 bp average fragment length
   5% of sequence in fragments shorter than    1413 bp
  10% of sequence in fragments shorter than    2652 bp
  15% of sequence in fragments shorter than    3839 bp
  20% of sequence in fragments shorter than    5008 bp
  25% of sequence in fragments shorter than    6203 bp
  30% of sequence in fragments shorter than    7444 bp
  35% of sequence in fragments shorter than    8765 bp
  40% of sequence in fragments shorter than   10187 bp
  45% of sequence in fragments shorter than   11714 bp
  50% of sequence in fragments shorter than   13383 bp
  55% of sequence in fragments shorter than   15255 bp
  60% of sequence in fragments shorter than   17320 bp
  65% of sequence in fragments shorter than   19698 bp
  70% of sequence in fragments shorter than   22450 bp
  75% of sequence in fragments shorter than   25598 bp
  80% of sequence in fragments shorter than   29567 bp
  85% of sequence in fragments shorter than   34606 bp
  90% of sequence in fragments shorter than   41843 bp
  95% of sequence in fragments shorter than   54721 bp

# SacI.SalI (GAGCTC+GTCGAC) double digest, 634 thousand fragments (603323+31396 sites), 4697 bp average fragment length
   5% of sequence in fragments shorter than    1595 bp
  10% of sequence in fragments shorter than    2447 bp
  15% of sequence in fragments shorter than    3219 bp
  20% of sequence in fragments shorter than    3948 bp
  25% of sequence in fragments shorter than    4661 bp
  30% of sequence in fragments shorter than    5380 bp
  35% of sequence in fragments shorter than    6121 bp
  40% of sequence in fragments shorter than    6899 bp
  45% of sequence in fragments shorter than    7714 bp
  50% of sequence in fragments shorter than    8581 bp
  55% of sequence in fragments shorter than    9532 bp
  60% of sequence in fragments shorter than   10580 bp
  65% of sequence in fragments shorter than   11741 bp
  70% of sequence in fragments shorter than   13068 bp
  75% of sequence in fragments shorter than   14633 bp
  80% of sequence in fragments shorter than   16536 bp
  85% of sequence in fragments shorter than   19037 bp
  90% of sequence in fragments shorter than   22868 bp
  95% of sequence in fragments shorter than   30292 bp

# BstEII.SexAI (GGTNACC+ACCWGGT) double digest, 633 thousand fragments (335865+297900 sites), 4704 bp average fragment length
   5% of sequence in fragments shorter than    1639 bp
  10% of sequence in fragments shorter than    2502 bp
  15% of sequence in fragments shorter than    3271 bp
  20% of sequence in fragments shorter than    4002 bp
  25% of sequence in fragments shorter than    4716 bp
  30% of sequence in fragments shorter than    5425 bp
  35% of sequence in fragments shorter than    6152 bp
  40% of sequence in fragments shorter than    6906 bp
  45% of sequence in fragments shorter than    7693 bp
  50% of sequence in fragments shorter than    8535 bp
  55% of sequence in fragments shorter than    9461 bp
  60% of sequence in fragments shorter than   10462 bp
  65% of sequence in fragments shorter than   11587 bp
  70% of sequence in fragments shorter than   12864 bp
  75% of sequence in fragments shorter than   14365 bp
  80% of sequence in fragments shorter than   16197 bp
  85% of sequence in fragments shorter than   18617 bp
  90% of sequence in fragments shorter than   22255 bp
  95% of sequence in fragments shorter than   29875 bp

# AclI.ApaI (AACGTT+GGGCCC) double digest, 632 thousand fragments (172706+460339 sites), 4710 bp average fragment length
   5% of sequence in fragments shorter than    1512 bp
  10% of sequence in fragments shorter than    2498 bp
  15% of sequence in fragments shorter than    3419 bp
  20% of sequence in fragments shorter than    4306 bp
  25% of sequence in fragments shorter than    5214 bp
  30% of sequence in fragments shorter than    6139 bp
  35% of sequence in fragments shorter than    7099 bp
  40% of sequence in fragments shorter than    8116 bp
  45% of sequence in fragments shorter than    9166 bp
  50% of sequence in fragments shorter than   10294 bp
  55% of sequence in fragments shorter than   11509 bp
  60% of sequence in fragments shorter than   12863 bp
  65% of sequence in fragments shorter than   14375 bp
  70% of sequence in fragments shorter than   16101 bp
  75% of sequence in fragments shorter than   18121 bp
  80% of sequence in fragments shorter than   20627 bp
  85% of sequence in fragments shorter than   23822 bp
  90% of sequence in fragments shorter than   28509 bp
  95% of sequence in fragments shorter than   37013 bp

# MfeI.SacII (CAATTG+CCGCGG) double digest, 630 thousand fragments (564303+66901 sites), 4724 bp average fragment length
   5% of sequence in fragments shorter than    1674 bp
  10% of sequence in fragments shorter than    2543 bp
  15% of sequence in fragments shorter than    3309 bp
  20% of sequence in fragments shorter than    4034 bp
  25% of sequence in fragments shorter than    4722 bp
  30% of sequence in fragments shorter than    5416 bp
  35% of sequence in fragments shorter than    6121 bp
  40% of sequence in fragments shorter than    6849 bp
  45% of sequence in fragments shorter than    7610 bp
  50% of sequence in fragments shorter than    8438 bp
  55% of sequence in fragments shorter than    9328 bp
  60% of sequence in fragments shorter than   10314 bp
  65% of sequence in fragments shorter than   11387 bp
  70% of sequence in fragments shorter than   12612 bp
  75% of sequence in fragments shorter than   14051 bp
  80% of sequence in fragments shorter than   15819 bp
  85% of sequence in fragments shorter than   18128 bp
  90% of sequence in fragments shorter than   21563 bp
  95% of sequence in fragments shorter than   28016 bp

# HgaI.PspXI (GACGC+VCTCGAGB) double digest, 630 thousand fragments (571889+59193 sites), 4725 bp average fragment length
   5% of sequence in fragments shorter than    1567 bp
  10% of sequence in fragments shorter than    2634 bp
  15% of sequence in fragments shorter than    3598 bp
  20% of sequence in fragments shorter than    4579 bp
  25% of sequence in fragments shorter than    5566 bp
  30% of sequence in fragments shorter than    6584 bp
  35% of sequence in fragments shorter than    7636 bp
  40% of sequence in fragments shorter than    8764 bp
  45% of sequence in fragments shorter than    9989 bp
  50% of sequence in fragments shorter than   11300 bp
  55% of sequence in fragments shorter than   12734 bp
  60% of sequence in fragments shorter than   14352 bp
  65% of sequence in fragments shorter than   16149 bp
  70% of sequence in fragments shorter than   18227 bp
  75% of sequence in fragments shorter than   20658 bp
  80% of sequence in fragments shorter than   23628 bp
  85% of sequence in fragments shorter than   27498 bp
  90% of sequence in fragments shorter than   33334 bp
  95% of sequence in fragments shorter than   44696 bp

# KasI.SpeI (GGCGCC+ACTAGT) double digest, 630 thousand fragments (230381+400286 sites), 4728 bp average fragment length
   5% of sequence in fragments shorter than    1665 bp
  10% of sequence in fragments shorter than    2523 bp
  15% of sequence in fragments shorter than    3283 bp
  20% of sequence in fragments shorter than    3996 bp
  25% of sequence in fragments shorter than    4694 bp
  30% of sequence in fragments shorter than    5328 bp
  35% of sequence in fragments shorter than    6033 bp
  40% of sequence in fragments shorter than    6758 bp
  45% of sequence in fragments shorter than    7518 bp
  50% of sequence in fragments shorter than    8328 bp
  55% of sequence in fragments shorter than    9198 bp
  60% of sequence in fragments shorter than   10164 bp
  65% of sequence in fragments shorter than   11234 bp
  70% of sequence in fragments shorter than   12417 bp
  75% of sequence in fragments shorter than   13813 bp
  80% of sequence in fragments shorter than   15549 bp
  85% of sequence in fragments shorter than   17774 bp
  90% of sequence in fragments shorter than   21025 bp
  95% of sequence in fragments shorter than   27194 bp

# EciI.PspXI (GGCGGA+VCTCGAGB) double digest, 630 thousand fragments (571273+59193 sites), 4729 bp average fragment length
   5% of sequence in fragments shorter than    1519 bp
  10% of sequence in fragments shorter than    2497 bp
  15% of sequence in fragments shorter than    3411 bp
  20% of sequence in fragments shorter than    4339 bp
  25% of sequence in fragments shorter than    5315 bp
  30% of sequence in fragments shorter than    6346 bp
  35% of sequence in fragments shorter than    7453 bp
  40% of sequence in fragments shorter than    8648 bp
  45% of sequence in fragments shorter than    9990 bp
  50% of sequence in fragments shorter than   11470 bp
  55% of sequence in fragments shorter than   13096 bp
  60% of sequence in fragments shorter than   14992 bp
  65% of sequence in fragments shorter than   17167 bp
  70% of sequence in fragments shorter than   19650 bp
  75% of sequence in fragments shorter than   22656 bp
  80% of sequence in fragments shorter than   26454 bp
  85% of sequence in fragments shorter than   31486 bp
  90% of sequence in fragments shorter than   39234 bp
  95% of sequence in fragments shorter than   54616 bp

# ClaI.ScaI (ATCGAT+AGTACT) double digest, 629 thousand fragments (86477+543793 sites), 4730 bp average fragment length
   5% of sequence in fragments shorter than    1646 bp
  10% of sequence in fragments shorter than    2481 bp
  15% of sequence in fragments shorter than    3207 bp
  20% of sequence in fragments shorter than    3895 bp
  25% of sequence in fragments shorter than    4571 bp
  30% of sequence in fragments shorter than    5245 bp
  35% of sequence in fragments shorter than    5944 bp
  40% of sequence in fragments shorter than    6667 bp
  45% of sequence in fragments shorter than    7437 bp
  50% of sequence in fragments shorter than    8241 bp
  55% of sequence in fragments shorter than    9121 bp
  60% of sequence in fragments shorter than   10073 bp
  65% of sequence in fragments shorter than   11153 bp
  70% of sequence in fragments shorter than   12412 bp
  75% of sequence in fragments shorter than   13886 bp
  80% of sequence in fragments shorter than   15715 bp
  85% of sequence in fragments shorter than   18134 bp
  90% of sequence in fragments shorter than   21861 bp
  95% of sequence in fragments shorter than   29657 bp

# HaeII.KasI (RGCGCY+GGCGCC) double digest, 629 thousand fragments (630101+230381 sites), 4732 bp average fragment length
   5% of sequence in fragments shorter than    1421 bp
  10% of sequence in fragments shorter than    2660 bp
  15% of sequence in fragments shorter than    3851 bp
  20% of sequence in fragments shorter than    5023 bp
  25% of sequence in fragments shorter than    6216 bp
  30% of sequence in fragments shorter than    7462 bp
  35% of sequence in fragments shorter than    8785 bp
  40% of sequence in fragments shorter than   10209 bp
  45% of sequence in fragments shorter than   11736 bp
  50% of sequence in fragments shorter than   13407 bp
  55% of sequence in fragments shorter than   15281 bp
  60% of sequence in fragments shorter than   17344 bp
  65% of sequence in fragments shorter than   19728 bp
  70% of sequence in fragments shorter than   22484 bp
  75% of sequence in fragments shorter than   25633 bp
  80% of sequence in fragments shorter than   29595 bp
  85% of sequence in fragments shorter than   34636 bp
  90% of sequence in fragments shorter than   41889 bp
  95% of sequence in fragments shorter than   54762 bp

# ApaLI.SnaBI (GTGCAC+TACGTA) double digest, 628 thousand fragments (496255+132299 sites), 4744 bp average fragment length
   5% of sequence in fragments shorter than    1696 bp
  10% of sequence in fragments shorter than    2567 bp
  15% of sequence in fragments shorter than    3308 bp
  20% of sequence in fragments shorter than    4020 bp
  25% of sequence in fragments shorter than    4707 bp
  30% of sequence in fragments shorter than    5406 bp
  35% of sequence in fragments shorter than    6076 bp
  40% of sequence in fragments shorter than    6797 bp
  45% of sequence in fragments shorter than    7552 bp
  50% of sequence in fragments shorter than    8344 bp
  55% of sequence in fragments shorter than    9195 bp
  60% of sequence in fragments shorter than   10113 bp
  65% of sequence in fragments shorter than   11158 bp
  70% of sequence in fragments shorter than   12327 bp
  75% of sequence in fragments shorter than   13725 bp
  80% of sequence in fragments shorter than   15434 bp
  85% of sequence in fragments shorter than   17615 bp
  90% of sequence in fragments shorter than   20792 bp
  95% of sequence in fragments shorter than   26746 bp

# SpeI.SwaI (ACTAGT+ATTTAAAT) double digest, 626 thousand fragments (400286+226602 sites), 4756 bp average fragment length
   5% of sequence in fragments shorter than    1626 bp
  10% of sequence in fragments shorter than    2459 bp
  15% of sequence in fragments shorter than    3201 bp
  20% of sequence in fragments shorter than    3914 bp
  25% of sequence in fragments shorter than    4619 bp
  30% of sequence in fragments shorter than    5334 bp
  35% of sequence in fragments shorter than    6065 bp
  40% of sequence in fragments shorter than    6811 bp
  45% of sequence in fragments shorter than    7619 bp
  50% of sequence in fragments shorter than    8493 bp
  55% of sequence in fragments shorter than    9448 bp
  60% of sequence in fragments shorter than   10519 bp
  65% of sequence in fragments shorter than   11737 bp
  70% of sequence in fragments shorter than   13157 bp
  75% of sequence in fragments shorter than   14896 bp
  80% of sequence in fragments shorter than   17166 bp
  85% of sequence in fragments shorter than   20291 bp
  90% of sequence in fragments shorter than   25361 bp
  95% of sequence in fragments shorter than   38200 bp

# AvrII.SalI (CCTAGG+GTCGAC) double digest, 625 thousand fragments (594956+31396 sites), 4760 bp average fragment length
   5% of sequence in fragments shorter than    1658 bp
  10% of sequence in fragments shorter than    2548 bp
  15% of sequence in fragments shorter than    3312 bp
  20% of sequence in fragments shorter than    4021 bp
  25% of sequence in fragments shorter than    4699 bp
  30% of sequence in fragments shorter than    5384 bp
  35% of sequence in fragments shorter than    6079 bp
  40% of sequence in fragments shorter than    6801 bp
  45% of sequence in fragments shorter than    7561 bp
  50% of sequence in fragments shorter than    8353 bp
  55% of sequence in fragments shorter than    9222 bp
  60% of sequence in fragments shorter than   10165 bp
  65% of sequence in fragments shorter than   11215 bp
  70% of sequence in fragments shorter than   12424 bp
  75% of sequence in fragments shorter than   13876 bp
  80% of sequence in fragments shorter than   15612 bp
  85% of sequence in fragments shorter than   17883 bp
  90% of sequence in fragments shorter than   21298 bp
  95% of sequence in fragments shorter than   28256 bp

# AgeI.HgaI (ACCGGT+GACGC) double digest, 624 thousand fragments (53386+571889 sites), 4768 bp average fragment length
   5% of sequence in fragments shorter than    1581 bp
  10% of sequence in fragments shorter than    2645 bp
  15% of sequence in fragments shorter than    3631 bp
  20% of sequence in fragments shorter than    4612 bp
  25% of sequence in fragments shorter than    5593 bp
  30% of sequence in fragments shorter than    6600 bp
  35% of sequence in fragments shorter than    7642 bp
  40% of sequence in fragments shorter than    8760 bp
  45% of sequence in fragments shorter than    9958 bp
  50% of sequence in fragments shorter than   11262 bp
  55% of sequence in fragments shorter than   12670 bp
  60% of sequence in fragments shorter than   14259 bp
  65% of sequence in fragments shorter than   16013 bp
  70% of sequence in fragments shorter than   18023 bp
  75% of sequence in fragments shorter than   20431 bp
  80% of sequence in fragments shorter than   23331 bp
  85% of sequence in fragments shorter than   27217 bp
  90% of sequence in fragments shorter than   32941 bp
  95% of sequence in fragments shorter than   43778 bp

# BsrBI.HpaI (CCGCTC+GTTAAC) double digest, 624 thousand fragments (235523+389418 sites), 4771 bp average fragment length
   5% of sequence in fragments shorter than    1685 bp
  10% of sequence in fragments shorter than    2596 bp
  15% of sequence in fragments shorter than    3382 bp
  20% of sequence in fragments shorter than    4115 bp
  25% of sequence in fragments shorter than    4838 bp
  30% of sequence in fragments shorter than    5559 bp
  35% of sequence in fragments shorter than    6297 bp
  40% of sequence in fragments shorter than    7050 bp
  45% of sequence in fragments shorter than    7854 bp
  50% of sequence in fragments shorter than    8689 bp
  55% of sequence in fragments shorter than    9591 bp
  60% of sequence in fragments shorter than   10565 bp
  65% of sequence in fragments shorter than   11668 bp
  70% of sequence in fragments shorter than   12906 bp
  75% of sequence in fragments shorter than   14338 bp
  80% of sequence in fragments shorter than   16068 bp
  85% of sequence in fragments shorter than   18307 bp
  90% of sequence in fragments shorter than   21535 bp
  95% of sequence in fragments shorter than   27788 bp

# MluI.SacI (ACGCGT+GAGCTC) double digest, 624 thousand fragments (21415+603323 sites), 4772 bp average fragment length
   5% of sequence in fragments shorter than    1616 bp
  10% of sequence in fragments shorter than    2483 bp
  15% of sequence in fragments shorter than    3266 bp
  20% of sequence in fragments shorter than    4016 bp
  25% of sequence in fragments shorter than    4740 bp
  30% of sequence in fragments shorter than    5472 bp
  35% of sequence in fragments shorter than    6236 bp
  40% of sequence in fragments shorter than    7023 bp
  45% of sequence in fragments shorter than    7850 bp
  50% of sequence in fragments shorter than    8743 bp
  55% of sequence in fragments shorter than    9714 bp
  60% of sequence in fragments shorter than   10789 bp
  65% of sequence in fragments shorter than   11980 bp
  70% of sequence in fragments shorter than   13354 bp
  75% of sequence in fragments shorter than   14956 bp
  80% of sequence in fragments shorter than   16929 bp
  85% of sequence in fragments shorter than   19580 bp
  90% of sequence in fragments shorter than   23548 bp
  95% of sequence in fragments shorter than   31198 bp

# AgeI.EciI (ACCGGT+GGCGGA) double digest, 624 thousand fragments (53386+571273 sites), 4773 bp average fragment length
   5% of sequence in fragments shorter than    1544 bp
  10% of sequence in fragments shorter than    2534 bp
  15% of sequence in fragments shorter than    3452 bp
  20% of sequence in fragments shorter than    4386 bp
  25% of sequence in fragments shorter than    5354 bp
  30% of sequence in fragments shorter than    6367 bp
  35% of sequence in fragments shorter than    7458 bp
  40% of sequence in fragments shorter than    8618 bp
  45% of sequence in fragments shorter than    9913 bp
  50% of sequence in fragments shorter than   11341 bp
  55% of sequence in fragments shorter than   12935 bp
  60% of sequence in fragments shorter than   14732 bp
  65% of sequence in fragments shorter than   16780 bp
  70% of sequence in fragments shorter than   19173 bp
  75% of sequence in fragments shorter than   22059 bp
  80% of sequence in fragments shorter than   25696 bp
  85% of sequence in fragments shorter than   30449 bp
  90% of sequence in fragments shorter than   37564 bp
  95% of sequence in fragments shorter than   51860 bp

# BstEII.KpnI (GGTNACC+GGTACC) double digest, 623 thousand fragments (335865+287974 sites), 4779 bp average fragment length
   5% of sequence in fragments shorter than    1583 bp
  10% of sequence in fragments shorter than    2440 bp
  15% of sequence in fragments shorter than    3218 bp
  20% of sequence in fragments shorter than    3968 bp
  25% of sequence in fragments shorter than    4701 bp
  30% of sequence in fragments shorter than    5427 bp
  35% of sequence in fragments shorter than    6173 bp
  40% of sequence in fragments shorter than    6944 bp
  45% of sequence in fragments shorter than    7757 bp
  50% of sequence in fragments shorter than    8616 bp
  55% of sequence in fragments shorter than    9539 bp
  60% of sequence in fragments shorter than   10573 bp
  65% of sequence in fragments shorter than   11696 bp
  70% of sequence in fragments shorter than   12998 bp
  75% of sequence in fragments shorter than   14509 bp
  80% of sequence in fragments shorter than   16368 bp
  85% of sequence in fragments shorter than   18793 bp
  90% of sequence in fragments shorter than   22530 bp
  95% of sequence in fragments shorter than   30101 bp

# MfeI.PspXI (CAATTG+VCTCGAGB) double digest, 623 thousand fragments (564303+59193 sites), 4782 bp average fragment length
   5% of sequence in fragments shorter than    1672 bp
  10% of sequence in fragments shorter than    2512 bp
  15% of sequence in fragments shorter than    3250 bp
  20% of sequence in fragments shorter than    3949 bp
  25% of sequence in fragments shorter than    4621 bp
  30% of sequence in fragments shorter than    5292 bp
  35% of sequence in fragments shorter than    5980 bp
  40% of sequence in fragments shorter than    6692 bp
  45% of sequence in fragments shorter than    7437 bp
  50% of sequence in fragments shorter than    8236 bp
  55% of sequence in fragments shorter than    9092 bp
  60% of sequence in fragments shorter than   10051 bp
  65% of sequence in fragments shorter than   11117 bp
  70% of sequence in fragments shorter than   12325 bp
  75% of sequence in fragments shorter than   13734 bp
  80% of sequence in fragments shorter than   15457 bp
  85% of sequence in fragments shorter than   17717 bp
  90% of sequence in fragments shorter than   21130 bp
  95% of sequence in fragments shorter than   27642 bp

# BstEII.NheI (GGTNACC+GCTAGC) double digest, 622 thousand fragments (335865+287384 sites), 4784 bp average fragment length
   5% of sequence in fragments shorter than    1622 bp
  10% of sequence in fragments shorter than    2428 bp
  15% of sequence in fragments shorter than    3220 bp
  20% of sequence in fragments shorter than    3966 bp
  25% of sequence in fragments shorter than    4702 bp
  30% of sequence in fragments shorter than    5425 bp
  35% of sequence in fragments shorter than    6172 bp
  40% of sequence in fragments shorter than    6935 bp
  45% of sequence in fragments shorter than    7735 bp
  50% of sequence in fragments shorter than    8596 bp
  55% of sequence in fragments shorter than    9505 bp
  60% of sequence in fragments shorter than   10509 bp
  65% of sequence in fragments shorter than   11613 bp
  70% of sequence in fragments shorter than   12894 bp
  75% of sequence in fragments shorter than   14390 bp
  80% of sequence in fragments shorter than   16209 bp
  85% of sequence in fragments shorter than   18575 bp
  90% of sequence in fragments shorter than   21989 bp
  95% of sequence in fragments shorter than   28918 bp

# AatII.SphI (GACGTC+GCATGC) double digest, 621 thousand fragments (71089+550952 sites), 4793 bp average fragment length
   5% of sequence in fragments shorter than    1668 bp
  10% of sequence in fragments shorter than    2524 bp
  15% of sequence in fragments shorter than    3277 bp
  20% of sequence in fragments shorter than    3987 bp
  25% of sequence in fragments shorter than    4688 bp
  30% of sequence in fragments shorter than    5394 bp
  35% of sequence in fragments shorter than    6111 bp
  40% of sequence in fragments shorter than    6859 bp
  45% of sequence in fragments shorter than    7639 bp
  50% of sequence in fragments shorter than    8465 bp
  55% of sequence in fragments shorter than    9367 bp
  60% of sequence in fragments shorter than   10364 bp
  65% of sequence in fragments shorter than   11500 bp
  70% of sequence in fragments shorter than   12770 bp
  75% of sequence in fragments shorter than   14269 bp
  80% of sequence in fragments shorter than   16114 bp
  85% of sequence in fragments shorter than   18494 bp
  90% of sequence in fragments shorter than   22016 bp
  95% of sequence in fragments shorter than   28891 bp

# ApaLI.NaeI (GTGCAC+GCCGGC) double digest, 621 thousand fragments (496255+125692 sites), 4794 bp average fragment length
   5% of sequence in fragments shorter than    1669 bp
  10% of sequence in fragments shorter than    2597 bp
  15% of sequence in fragments shorter than    3422 bp
  20% of sequence in fragments shorter than    4205 bp
  25% of sequence in fragments shorter than    4983 bp
  30% of sequence in fragments shorter than    5759 bp
  35% of sequence in fragments shorter than    6534 bp
  40% of sequence in fragments shorter than    7369 bp
  45% of sequence in fragments shorter than    8252 bp
  50% of sequence in fragments shorter than    9196 bp
  55% of sequence in fragments shorter than   10209 bp
  60% of sequence in fragments shorter than   11331 bp
  65% of sequence in fragments shorter than   12590 bp
  70% of sequence in fragments shorter than   14055 bp
  75% of sequence in fragments shorter than   15770 bp
  80% of sequence in fragments shorter than   17878 bp
  85% of sequence in fragments shorter than   20664 bp
  90% of sequence in fragments shorter than   24659 bp
  95% of sequence in fragments shorter than   32625 bp

# ApaI.PacI (GGGCCC+TTAATTAA) double digest, 619 thousand fragments (460339+159455 sites), 4810 bp average fragment length
   5% of sequence in fragments shorter than    1606 bp
  10% of sequence in fragments shorter than    2634 bp
  15% of sequence in fragments shorter than    3572 bp
  20% of sequence in fragments shorter than    4491 bp
  25% of sequence in fragments shorter than    5409 bp
  30% of sequence in fragments shorter than    6345 bp
  35% of sequence in fragments shorter than    7299 bp
  40% of sequence in fragments shorter than    8309 bp
  45% of sequence in fragments shorter than    9367 bp
  50% of sequence in fragments shorter than   10491 bp
  55% of sequence in fragments shorter than   11729 bp
  60% of sequence in fragments shorter than   13062 bp
  65% of sequence in fragments shorter than   14546 bp
  70% of sequence in fragments shorter than   16260 bp
  75% of sequence in fragments shorter than   18276 bp
  80% of sequence in fragments shorter than   20799 bp
  85% of sequence in fragments shorter than   24032 bp
  90% of sequence in fragments shorter than   28976 bp
  95% of sequence in fragments shorter than   38995 bp

# HpaI.KasI (GTTAAC+GGCGCC) double digest, 619 thousand fragments (389418+230381 sites), 4810 bp average fragment length
   5% of sequence in fragments shorter than    1687 bp
  10% of sequence in fragments shorter than    2577 bp
  15% of sequence in fragments shorter than    3361 bp
  20% of sequence in fragments shorter than    4088 bp
  25% of sequence in fragments shorter than    4808 bp
  30% of sequence in fragments shorter than    5524 bp
  35% of sequence in fragments shorter than    6256 bp
  40% of sequence in fragments shorter than    7004 bp
  45% of sequence in fragments shorter than    7794 bp
  50% of sequence in fragments shorter than    8638 bp
  55% of sequence in fragments shorter than    9546 bp
  60% of sequence in fragments shorter than   10527 bp
  65% of sequence in fragments shorter than   11653 bp
  70% of sequence in fragments shorter than   12936 bp
  75% of sequence in fragments shorter than   14435 bp
  80% of sequence in fragments shorter than   16269 bp
  85% of sequence in fragments shorter than   18615 bp
  90% of sequence in fragments shorter than   22118 bp
  95% of sequence in fragments shorter than   29137 bp

# AclI.EcoRV (AACGTT+GATATC) double digest, 618 thousand fragments (172706+446473 sites), 4816 bp average fragment length
   5% of sequence in fragments shorter than    1650 bp
  10% of sequence in fragments shorter than    2497 bp
  15% of sequence in fragments shorter than    3279 bp
  20% of sequence in fragments shorter than    4012 bp
  25% of sequence in fragments shorter than    4730 bp
  30% of sequence in fragments shorter than    5442 bp
  35% of sequence in fragments shorter than    6170 bp
  40% of sequence in fragments shorter than    6922 bp
  45% of sequence in fragments shorter than    7722 bp
  50% of sequence in fragments shorter than    8575 bp
  55% of sequence in fragments shorter than    9493 bp
  60% of sequence in fragments shorter than   10490 bp
  65% of sequence in fragments shorter than   11615 bp
  70% of sequence in fragments shorter than   12906 bp
  75% of sequence in fragments shorter than   14423 bp
  80% of sequence in fragments shorter than   16292 bp
  85% of sequence in fragments shorter than   18768 bp
  90% of sequence in fragments shorter than   22415 bp
  95% of sequence in fragments shorter than   29446 bp

# SacI.SgrAI (GAGCTC+CRCCGGYG) double digest, 618 thousand fragments (603323+15270 sites), 4820 bp average fragment length
   5% of sequence in fragments shorter than    1632 bp
  10% of sequence in fragments shorter than    2512 bp
  15% of sequence in fragments shorter than    3303 bp
  20% of sequence in fragments shorter than    4062 bp
  25% of sequence in fragments shorter than    4797 bp
  30% of sequence in fragments shorter than    5538 bp
  35% of sequence in fragments shorter than    6307 bp
  40% of sequence in fragments shorter than    7105 bp
  45% of sequence in fragments shorter than    7944 bp
  50% of sequence in fragments shorter than    8845 bp
  55% of sequence in fragments shorter than    9828 bp
  60% of sequence in fragments shorter than   10916 bp
  65% of sequence in fragments shorter than   12123 bp
  70% of sequence in fragments shorter than   13518 bp
  75% of sequence in fragments shorter than   15132 bp
  80% of sequence in fragments shorter than   17095 bp
  85% of sequence in fragments shorter than   19777 bp
  90% of sequence in fragments shorter than   23830 bp
  95% of sequence in fragments shorter than   31557 bp

# ApaI.Hpy99I (GGGCCC+CGWCG) double digest, 617 thousand fragments (460339+157917 sites), 4822 bp average fragment length
   5% of sequence in fragments shorter than    1535 bp
  10% of sequence in fragments shorter than    2711 bp
  15% of sequence in fragments shorter than    3858 bp
  20% of sequence in fragments shorter than    5017 bp
  25% of sequence in fragments shorter than    6225 bp
  30% of sequence in fragments shorter than    7470 bp
  35% of sequence in fragments shorter than    8773 bp
  40% of sequence in fragments shorter than   10157 bp
  45% of sequence in fragments shorter than   11648 bp
  50% of sequence in fragments shorter than   13261 bp
  55% of sequence in fragments shorter than   15033 bp
  60% of sequence in fragments shorter than   16984 bp
  65% of sequence in fragments shorter than   19216 bp
  70% of sequence in fragments shorter than   21858 bp
  75% of sequence in fragments shorter than   24932 bp
  80% of sequence in fragments shorter than   28870 bp
  85% of sequence in fragments shorter than   34007 bp
  90% of sequence in fragments shorter than   41710 bp
  95% of sequence in fragments shorter than   56873 bp

# NruI.SacI (TCGCGA+GAGCTC) double digest, 617 thousand fragments (14872+603323 sites), 4823 bp average fragment length
   5% of sequence in fragments shorter than    1636 bp
  10% of sequence in fragments shorter than    2512 bp
  15% of sequence in fragments shorter than    3300 bp
  20% of sequence in fragments shorter than    4054 bp
  25% of sequence in fragments shorter than    4785 bp
  30% of sequence in fragments shorter than    5524 bp
  35% of sequence in fragments shorter than    6292 bp
  40% of sequence in fragments shorter than    7085 bp
  45% of sequence in fragments shorter than    7919 bp
  50% of sequence in fragments shorter than    8816 bp
  55% of sequence in fragments shorter than    9795 bp
  60% of sequence in fragments shorter than   10876 bp
  65% of sequence in fragments shorter than   12068 bp
  70% of sequence in fragments shorter than   13451 bp
  75% of sequence in fragments shorter than   15054 bp
  80% of sequence in fragments shorter than   17015 bp
  85% of sequence in fragments shorter than   19693 bp
  90% of sequence in fragments shorter than   23703 bp
  95% of sequence in fragments shorter than   31388 bp

# SacII.SphI (CCGCGG+GCATGC) double digest, 617 thousand fragments (66901+550952 sites), 4826 bp average fragment length
   5% of sequence in fragments shorter than    1698 bp
  10% of sequence in fragments shorter than    2599 bp
  15% of sequence in fragments shorter than    3395 bp
  20% of sequence in fragments shorter than    4148 bp
  25% of sequence in fragments shorter than    4892 bp
  30% of sequence in fragments shorter than    5637 bp
  35% of sequence in fragments shorter than    6400 bp
  40% of sequence in fragments shorter than    7187 bp
  45% of sequence in fragments shorter than    8012 bp
  50% of sequence in fragments shorter than    8893 bp
  55% of sequence in fragments shorter than    9861 bp
  60% of sequence in fragments shorter than   10943 bp
  65% of sequence in fragments shorter than   12142 bp
  70% of sequence in fragments shorter than   13514 bp
  75% of sequence in fragments shorter than   15125 bp
  80% of sequence in fragments shorter than   17101 bp
  85% of sequence in fragments shorter than   19691 bp
  90% of sequence in fragments shorter than   23501 bp
  95% of sequence in fragments shorter than   31100 bp

# AgeI.MfeI (ACCGGT+CAATTG) double digest, 617 thousand fragments (53386+564303 sites), 4827 bp average fragment length
   5% of sequence in fragments shorter than    1686 bp
  10% of sequence in fragments shorter than    2496 bp
  15% of sequence in fragments shorter than    3246 bp
  20% of sequence in fragments shorter than    3958 bp
  25% of sequence in fragments shorter than    4643 bp
  30% of sequence in fragments shorter than    5332 bp
  35% of sequence in fragments shorter than    6034 bp
  40% of sequence in fragments shorter than    6764 bp
  45% of sequence in fragments shorter than    7534 bp
  50% of sequence in fragments shorter than    8366 bp
  55% of sequence in fragments shorter than    9267 bp
  60% of sequence in fragments shorter than   10266 bp
  65% of sequence in fragments shorter than   11366 bp
  70% of sequence in fragments shorter than   12631 bp
  75% of sequence in fragments shorter than   14116 bp
  80% of sequence in fragments shorter than   15980 bp
  85% of sequence in fragments shorter than   18473 bp
  90% of sequence in fragments shorter than   22136 bp
  95% of sequence in fragments shorter than   29480 bp

# ApaLI.XhoI (GTGCAC+CTCGAG) double digest, 617 thousand fragments (496255+121308 sites), 4828 bp average fragment length
   5% of sequence in fragments shorter than    1690 bp
  10% of sequence in fragments shorter than    2583 bp
  15% of sequence in fragments shorter than    3367 bp
  20% of sequence in fragments shorter than    4099 bp
  25% of sequence in fragments shorter than    4748 bp
  30% of sequence in fragments shorter than    5464 bp
  35% of sequence in fragments shorter than    6199 bp
  40% of sequence in fragments shorter than    6974 bp
  45% of sequence in fragments shorter than    7801 bp
  50% of sequence in fragments shorter than    8670 bp
  55% of sequence in fragments shorter than    9607 bp
  60% of sequence in fragments shorter than   10649 bp
  65% of sequence in fragments shorter than   11806 bp
  70% of sequence in fragments shorter than   13148 bp
  75% of sequence in fragments shorter than   14710 bp
  80% of sequence in fragments shorter than   16639 bp
  85% of sequence in fragments shorter than   19138 bp
  90% of sequence in fragments shorter than   22826 bp
  95% of sequence in fragments shorter than   30113 bp

# PvuI.SacI (CGATCG+GAGCTC) double digest, 617 thousand fragments (14186+603323 sites), 4828 bp average fragment length
   5% of sequence in fragments shorter than    1639 bp
  10% of sequence in fragments shorter than    2506 bp
  15% of sequence in fragments shorter than    3298 bp
  20% of sequence in fragments shorter than    4056 bp
  25% of sequence in fragments shorter than    4789 bp
  30% of sequence in fragments shorter than    5531 bp
  35% of sequence in fragments shorter than    6299 bp
  40% of sequence in fragments shorter than    7091 bp
  45% of sequence in fragments shorter than    7927 bp
  50% of sequence in fragments shorter than    8821 bp
  55% of sequence in fragments shorter than    9802 bp
  60% of sequence in fragments shorter than   10885 bp
  65% of sequence in fragments shorter than   12077 bp
  70% of sequence in fragments shorter than   13455 bp
  75% of sequence in fragments shorter than   15064 bp
  80% of sequence in fragments shorter than   17024 bp
  85% of sequence in fragments shorter than   19675 bp
  90% of sequence in fragments shorter than   23650 bp
  95% of sequence in fragments shorter than   31145 bp

# FseI.SacI (GGCCGGCC+GAGCTC) double digest, 616 thousand fragments (13662+603323 sites), 4832 bp average fragment length
   5% of sequence in fragments shorter than    1635 bp
  10% of sequence in fragments shorter than    2515 bp
  15% of sequence in fragments shorter than    3307 bp
  20% of sequence in fragments shorter than    4069 bp
  25% of sequence in fragments shorter than    4806 bp
  30% of sequence in fragments shorter than    5552 bp
  35% of sequence in fragments shorter than    6326 bp
  40% of sequence in fragments shorter than    7125 bp
  45% of sequence in fragments shorter than    7969 bp
  50% of sequence in fragments shorter than    8871 bp
  55% of sequence in fragments shorter than    9867 bp
  60% of sequence in fragments shorter than   10955 bp
  65% of sequence in fragments shorter than   12167 bp
  70% of sequence in fragments shorter than   13564 bp
  75% of sequence in fragments shorter than   15190 bp
  80% of sequence in fragments shorter than   17180 bp
  85% of sequence in fragments shorter than   19876 bp
  90% of sequence in fragments shorter than   23946 bp
  95% of sequence in fragments shorter than   31704 bp

# AvrII.MluI (CCTAGG+ACGCGT) double digest, 616 thousand fragments (594956+21415 sites), 4837 bp average fragment length
   5% of sequence in fragments shorter than    1683 bp
  10% of sequence in fragments shorter than    2589 bp
  15% of sequence in fragments shorter than    3369 bp
  20% of sequence in fragments shorter than    4091 bp
  25% of sequence in fragments shorter than    4784 bp
  30% of sequence in fragments shorter than    5484 bp
  35% of sequence in fragments shorter than    6194 bp
  40% of sequence in fragments shorter than    6929 bp
  45% of sequence in fragments shorter than    7709 bp
  50% of sequence in fragments shorter than    8519 bp
  55% of sequence in fragments shorter than    9408 bp
  60% of sequence in fragments shorter than   10369 bp
  65% of sequence in fragments shorter than   11448 bp
  70% of sequence in fragments shorter than   12689 bp
  75% of sequence in fragments shorter than   14162 bp
  80% of sequence in fragments shorter than   15948 bp
  85% of sequence in fragments shorter than   18282 bp
  90% of sequence in fragments shorter than   21815 bp
  95% of sequence in fragments shorter than   29051 bp

# HpaI.SwaI (GTTAAC+ATTTAAAT) double digest, 615 thousand fragments (389418+226602 sites), 4840 bp average fragment length
   5% of sequence in fragments shorter than    1668 bp
  10% of sequence in fragments shorter than    2549 bp
  15% of sequence in fragments shorter than    3307 bp
  20% of sequence in fragments shorter than    4023 bp
  25% of sequence in fragments shorter than    4729 bp
  30% of sequence in fragments shorter than    5447 bp
  35% of sequence in fragments shorter than    6190 bp
  40% of sequence in fragments shorter than    6967 bp
  45% of sequence in fragments shorter than    7790 bp
  50% of sequence in fragments shorter than    8671 bp
  55% of sequence in fragments shorter than    9633 bp
  60% of sequence in fragments shorter than   10689 bp
  65% of sequence in fragments shorter than   11901 bp
  70% of sequence in fragments shorter than   13285 bp
  75% of sequence in fragments shorter than   14999 bp
  80% of sequence in fragments shorter than   17175 bp
  85% of sequence in fragments shorter than   20163 bp
  90% of sequence in fragments shorter than   24854 bp
  95% of sequence in fragments shorter than   36235 bp

# AatII.ScaI (GACGTC+AGTACT) double digest, 614 thousand fragments (71089+543793 sites), 4849 bp average fragment length
   5% of sequence in fragments shorter than    1691 bp
  10% of sequence in fragments shorter than    2551 bp
  15% of sequence in fragments shorter than    3300 bp
  20% of sequence in fragments shorter than    4009 bp
  25% of sequence in fragments shorter than    4704 bp
  30% of sequence in fragments shorter than    5403 bp
  35% of sequence in fragments shorter than    6114 bp
  40% of sequence in fragments shorter than    6854 bp
  45% of sequence in fragments shorter than    7626 bp
  50% of sequence in fragments shorter than    8440 bp
  55% of sequence in fragments shorter than    9318 bp
  60% of sequence in fragments shorter than   10270 bp
  65% of sequence in fragments shorter than   11347 bp
  70% of sequence in fragments shorter than   12582 bp
  75% of sequence in fragments shorter than   14034 bp
  80% of sequence in fragments shorter than   15772 bp
  85% of sequence in fragments shorter than   18047 bp
  90% of sequence in fragments shorter than   21415 bp
  95% of sequence in fragments shorter than   28165 bp

# HgaI.PmeI (GACGC+GTTTAAAC) double digest, 613 thousand fragments (571889+42094 sites), 4856 bp average fragment length
   5% of sequence in fragments shorter than    1639 bp
  10% of sequence in fragments shorter than    2716 bp
  15% of sequence in fragments shorter than    3680 bp
  20% of sequence in fragments shorter than    4654 bp
  25% of sequence in fragments shorter than    5626 bp
  30% of sequence in fragments shorter than    6612 bp
  35% of sequence in fragments shorter than    7623 bp
  40% of sequence in fragments shorter than    8712 bp
  45% of sequence in fragments shorter than    9867 bp
  50% of sequence in fragments shorter than   11118 bp
  55% of sequence in fragments shorter than   12483 bp
  60% of sequence in fragments shorter than   14014 bp
  65% of sequence in fragments shorter than   15688 bp
  70% of sequence in fragments shorter than   17598 bp
  75% of sequence in fragments shorter than   19886 bp
  80% of sequence in fragments shorter than   22626 bp
  85% of sequence in fragments shorter than   26282 bp
  90% of sequence in fragments shorter than   31597 bp
  95% of sequence in fragments shorter than   41660 bp

# EciI.PmeI (GGCGGA+GTTTAAAC) double digest, 613 thousand fragments (571273+42094 sites), 4861 bp average fragment length
   5% of sequence in fragments shorter than    1582 bp
  10% of sequence in fragments shorter than    2583 bp
  15% of sequence in fragments shorter than    3511 bp
  20% of sequence in fragments shorter than    4450 bp
  25% of sequence in fragments shorter than    5424 bp
  30% of sequence in fragments shorter than    6449 bp
  35% of sequence in fragments shorter than    7533 bp
  40% of sequence in fragments shorter than    8703 bp
  45% of sequence in fragments shorter than   10003 bp
  50% of sequence in fragments shorter than   11410 bp
  55% of sequence in fragments shorter than   12984 bp
  60% of sequence in fragments shorter than   14771 bp
  65% of sequence in fragments shorter than   16793 bp
  70% of sequence in fragments shorter than   19185 bp
  75% of sequence in fragments shorter than   21985 bp
  80% of sequence in fragments shorter than   25527 bp
  85% of sequence in fragments shorter than   30168 bp
  90% of sequence in fragments shorter than   37362 bp
  95% of sequence in fragments shorter than   51412 bp

# NotI.SacI (GCGGCCGC+GAGCTC) double digest, 612 thousand fragments (9795+603323 sites), 4863 bp average fragment length
   5% of sequence in fragments shorter than    1649 bp
  10% of sequence in fragments shorter than    2536 bp
  15% of sequence in fragments shorter than    3335 bp
  20% of sequence in fragments shorter than    4102 bp
  25% of sequence in fragments shorter than    4840 bp
  30% of sequence in fragments shorter than    5590 bp
  35% of sequence in fragments shorter than    6364 bp
  40% of sequence in fragments shorter than    7167 bp
  45% of sequence in fragments shorter than    8020 bp
  50% of sequence in fragments shorter than    8925 bp
  55% of sequence in fragments shorter than    9928 bp
  60% of sequence in fragments shorter than   11018 bp
  65% of sequence in fragments shorter than   12231 bp
  70% of sequence in fragments shorter than   13632 bp
  75% of sequence in fragments shorter than   15260 bp
  80% of sequence in fragments shorter than   17266 bp
  85% of sequence in fragments shorter than   19962 bp
  90% of sequence in fragments shorter than   24061 bp
  95% of sequence in fragments shorter than   31819 bp

# RsrII.SacI (CGGWCCG+GAGCTC) double digest, 612 thousand fragments (9764+603323 sites), 4863 bp average fragment length
   5% of sequence in fragments shorter than    1648 bp
  10% of sequence in fragments shorter than    2532 bp
  15% of sequence in fragments shorter than    3329 bp
  20% of sequence in fragments shorter than    4093 bp
  25% of sequence in fragments shorter than    4830 bp
  30% of sequence in fragments shorter than    5577 bp
  35% of sequence in fragments shorter than    6351 bp
  40% of sequence in fragments shorter than    7153 bp
  45% of sequence in fragments shorter than    8000 bp
  50% of sequence in fragments shorter than    8907 bp
  55% of sequence in fragments shorter than    9899 bp
  60% of sequence in fragments shorter than   10994 bp
  65% of sequence in fragments shorter than   12199 bp
  70% of sequence in fragments shorter than   13598 bp
  75% of sequence in fragments shorter than   15228 bp
  80% of sequence in fragments shorter than   17227 bp
  85% of sequence in fragments shorter than   19922 bp
  90% of sequence in fragments shorter than   23992 bp
  95% of sequence in fragments shorter than   31791 bp

# BsrBI.SapI (CCGCTC+GCTCTTC) double digest, 612 thousand fragments (235523+377161 sites), 4867 bp average fragment length
   5% of sequence in fragments shorter than    1649 bp
  10% of sequence in fragments shorter than    2638 bp
  15% of sequence in fragments shorter than    3516 bp
  20% of sequence in fragments shorter than    4350 bp
  25% of sequence in fragments shorter than    5169 bp
  30% of sequence in fragments shorter than    5987 bp
  35% of sequence in fragments shorter than    6836 bp
  40% of sequence in fragments shorter than    7725 bp
  45% of sequence in fragments shorter than    8655 bp
  50% of sequence in fragments shorter than    9670 bp
  55% of sequence in fragments shorter than   10745 bp
  60% of sequence in fragments shorter than   11950 bp
  65% of sequence in fragments shorter than   13268 bp
  70% of sequence in fragments shorter than   14784 bp
  75% of sequence in fragments shorter than   16537 bp
  80% of sequence in fragments shorter than   18735 bp
  85% of sequence in fragments shorter than   21609 bp
  90% of sequence in fragments shorter than   25768 bp
  95% of sequence in fragments shorter than   33812 bp

# BsrBI.SmaI (CCGCTC+CCCGGG) double digest, 612 thousand fragments (235523+376940 sites), 4868 bp average fragment length
   5% of sequence in fragments shorter than    1571 bp
  10% of sequence in fragments shorter than    2650 bp
  15% of sequence in fragments shorter than    3686 bp
  20% of sequence in fragments shorter than    4744 bp
  25% of sequence in fragments shorter than    5856 bp
  30% of sequence in fragments shorter than    7036 bp
  35% of sequence in fragments shorter than    8315 bp
  40% of sequence in fragments shorter than    9681 bp
  45% of sequence in fragments shorter than   11167 bp
  50% of sequence in fragments shorter than   12826 bp
  55% of sequence in fragments shorter than   14696 bp
  60% of sequence in fragments shorter than   16741 bp
  65% of sequence in fragments shorter than   19139 bp
  70% of sequence in fragments shorter than   21880 bp
  75% of sequence in fragments shorter than   25235 bp
  80% of sequence in fragments shorter than   29477 bp
  85% of sequence in fragments shorter than   35049 bp
  90% of sequence in fragments shorter than   43127 bp
  95% of sequence in fragments shorter than   59062 bp

# SacII.ScaI (CCGCGG+AGTACT) double digest, 610 thousand fragments (66901+543793 sites), 4882 bp average fragment length
   5% of sequence in fragments shorter than    1718 bp
  10% of sequence in fragments shorter than    2622 bp
  15% of sequence in fragments shorter than    3417 bp
  20% of sequence in fragments shorter than    4167 bp
  25% of sequence in fragments shorter than    4893 bp
  30% of sequence in fragments shorter than    5630 bp
  35% of sequence in fragments shorter than    6381 bp
  40% of sequence in fragments shorter than    7163 bp
  45% of sequence in fragments shorter than    7971 bp
  50% of sequence in fragments shorter than    8829 bp
  55% of sequence in fragments shorter than    9744 bp
  60% of sequence in fragments shorter than   10762 bp
  65% of sequence in fragments shorter than   11898 bp
  70% of sequence in fragments shorter than   13191 bp
  75% of sequence in fragments shorter than   14717 bp
  80% of sequence in fragments shorter than   16548 bp
  85% of sequence in fragments shorter than   18957 bp
  90% of sequence in fragments shorter than   22550 bp
  95% of sequence in fragments shorter than   29793 bp

# AvrII.SgrAI (CCTAGG+CRCCGGYG) double digest, 609 thousand fragments (594956+15270 sites), 4886 bp average fragment length
   5% of sequence in fragments shorter than    1700 bp
  10% of sequence in fragments shorter than    2617 bp
  15% of sequence in fragments shorter than    3406 bp
  20% of sequence in fragments shorter than    4138 bp
  25% of sequence in fragments shorter than    4841 bp
  30% of sequence in fragments shorter than    5546 bp
  35% of sequence in fragments shorter than    6264 bp
  40% of sequence in fragments shorter than    7008 bp
  45% of sequence in fragments shorter than    7796 bp
  50% of sequence in fragments shorter than    8614 bp
  55% of sequence in fragments shorter than    9513 bp
  60% of sequence in fragments shorter than   10483 bp
  65% of sequence in fragments shorter than   11571 bp
  70% of sequence in fragments shorter than   12842 bp
  75% of sequence in fragments shorter than   14317 bp
  80% of sequence in fragments shorter than   16127 bp
  85% of sequence in fragments shorter than   18478 bp
  90% of sequence in fragments shorter than   22078 bp
  95% of sequence in fragments shorter than   29428 bp

# PspXI.SphI (VCTCGAGB+GCATGC) double digest, 609 thousand fragments (59193+550952 sites), 4887 bp average fragment length
   5% of sequence in fragments shorter than    1696 bp
  10% of sequence in fragments shorter than    2571 bp
  15% of sequence in fragments shorter than    3339 bp
  20% of sequence in fragments shorter than    4064 bp
  25% of sequence in fragments shorter than    4786 bp
  30% of sequence in fragments shorter than    5506 bp
  35% of sequence in fragments shorter than    6240 bp
  40% of sequence in fragments shorter than    6998 bp
  45% of sequence in fragments shorter than    7793 bp
  50% of sequence in fragments shorter than    8644 bp
  55% of sequence in fragments shorter than    9572 bp
  60% of sequence in fragments shorter than   10598 bp
  65% of sequence in fragments shorter than   11759 bp
  70% of sequence in fragments shorter than   13060 bp
  75% of sequence in fragments shorter than   14607 bp
  80% of sequence in fragments shorter than   16497 bp
  85% of sequence in fragments shorter than   18973 bp
  90% of sequence in fragments shorter than   22647 bp
  95% of sequence in fragments shorter than   29875 bp

# AvrII.NruI (CCTAGG+TCGCGA) double digest, 609 thousand fragments (594956+14872 sites), 4889 bp average fragment length
   5% of sequence in fragments shorter than    1702 bp
  10% of sequence in fragments shorter than    2619 bp
  15% of sequence in fragments shorter than    3404 bp
  20% of sequence in fragments shorter than    4133 bp
  25% of sequence in fragments shorter than    4833 bp
  30% of sequence in fragments shorter than    5537 bp
  35% of sequence in fragments shorter than    6257 bp
  40% of sequence in fragments shorter than    6998 bp
  45% of sequence in fragments shorter than    7782 bp
  50% of sequence in fragments shorter than    8599 bp
  55% of sequence in fragments shorter than    9495 bp
  60% of sequence in fragments shorter than   10460 bp
  65% of sequence in fragments shorter than   11548 bp
  70% of sequence in fragments shorter than   12814 bp
  75% of sequence in fragments shorter than   14284 bp
  80% of sequence in fragments shorter than   16090 bp
  85% of sequence in fragments shorter than   18428 bp
  90% of sequence in fragments shorter than   21976 bp
  95% of sequence in fragments shorter than   29213 bp

# AvrII.PvuI (CCTAGG+CGATCG) double digest, 608 thousand fragments (594956+14186 sites), 4894 bp average fragment length
   5% of sequence in fragments shorter than    1706 bp
  10% of sequence in fragments shorter than    2614 bp
  15% of sequence in fragments shorter than    3403 bp
  20% of sequence in fragments shorter than    4134 bp
  25% of sequence in fragments shorter than    4839 bp
  30% of sequence in fragments shorter than    5547 bp
  35% of sequence in fragments shorter than    6270 bp
  40% of sequence in fragments shorter than    7011 bp
  45% of sequence in fragments shorter than    7800 bp
  50% of sequence in fragments shorter than    8616 bp
  55% of sequence in fragments shorter than    9517 bp
  60% of sequence in fragments shorter than   10494 bp
  65% of sequence in fragments shorter than   11581 bp
  70% of sequence in fragments shorter than   12848 bp
  75% of sequence in fragments shorter than   14321 bp
  80% of sequence in fragments shorter than   16114 bp
  85% of sequence in fragments shorter than   18452 bp
  90% of sequence in fragments shorter than   21982 bp
  95% of sequence in fragments shorter than   29088 bp

# AvrII.FseI (CCTAGG+GGCCGGCC) double digest, 608 thousand fragments (594956+13662 sites), 4899 bp average fragment length
   5% of sequence in fragments shorter than    1705 bp
  10% of sequence in fragments shorter than    2623 bp
  15% of sequence in fragments shorter than    3414 bp
  20% of sequence in fragments shorter than    4146 bp
  25% of sequence in fragments shorter than    4849 bp
  30% of sequence in fragments shorter than    5558 bp
  35% of sequence in fragments shorter than    6281 bp
  40% of sequence in fragments shorter than    7025 bp
  45% of sequence in fragments shorter than    7815 bp
  50% of sequence in fragments shorter than    8635 bp
  55% of sequence in fragments shorter than    9538 bp
  60% of sequence in fragments shorter than   10513 bp
  65% of sequence in fragments shorter than   11602 bp
  70% of sequence in fragments shorter than   12885 bp
  75% of sequence in fragments shorter than   14362 bp
  80% of sequence in fragments shorter than   16191 bp
  85% of sequence in fragments shorter than   18548 bp
  90% of sequence in fragments shorter than   22152 bp
  95% of sequence in fragments shorter than   29579 bp

# AscI.SacI (GGCGCGCC+GAGCTC) double digest, 607 thousand fragments (4751+603323 sites), 4903 bp average fragment length
   5% of sequence in fragments shorter than    1664 bp
  10% of sequence in fragments shorter than    2554 bp
  15% of sequence in fragments shorter than    3357 bp
  20% of sequence in fragments shorter than    4127 bp
  25% of sequence in fragments shorter than    4867 bp
  30% of sequence in fragments shorter than    5621 bp
  35% of sequence in fragments shorter than    6397 bp
  40% of sequence in fragments shorter than    7202 bp
  45% of sequence in fragments shorter than    8052 bp
  50% of sequence in fragments shorter than    8958 bp
  55% of sequence in fragments shorter than    9964 bp
  60% of sequence in fragments shorter than   11059 bp
  65% of sequence in fragments shorter than   12271 bp
  70% of sequence in fragments shorter than   13670 bp
  75% of sequence in fragments shorter than   15302 bp
  80% of sequence in fragments shorter than   17311 bp
  85% of sequence in fragments shorter than   20014 bp
  90% of sequence in fragments shorter than   24095 bp
  95% of sequence in fragments shorter than   31886 bp

# KasI.SapI (GGCGCC+GCTCTTC) double digest, 607 thousand fragments (230381+377161 sites), 4907 bp average fragment length
   5% of sequence in fragments shorter than    1646 bp
  10% of sequence in fragments shorter than    2603 bp
  15% of sequence in fragments shorter than    3444 bp
  20% of sequence in fragments shorter than    4238 bp
  25% of sequence in fragments shorter than    5009 bp
  30% of sequence in fragments shorter than    5790 bp
  35% of sequence in fragments shorter than    6589 bp
  40% of sequence in fragments shorter than    7432 bp
  45% of sequence in fragments shorter than    8309 bp
  50% of sequence in fragments shorter than    9237 bp
  55% of sequence in fragments shorter than   10249 bp
  60% of sequence in fragments shorter than   11377 bp
  65% of sequence in fragments shorter than   12649 bp
  70% of sequence in fragments shorter than   14092 bp
  75% of sequence in fragments shorter than   15850 bp
  80% of sequence in fragments shorter than   17920 bp
  85% of sequence in fragments shorter than   20748 bp
  90% of sequence in fragments shorter than   24909 bp
  95% of sequence in fragments shorter than   33485 bp

# KasI.SmaI (GGCGCC+CCCGGG) double digest, 607 thousand fragments (230381+376940 sites), 4909 bp average fragment length
   5% of sequence in fragments shorter than    1648 bp
  10% of sequence in fragments shorter than    2802 bp
  15% of sequence in fragments shorter than    3919 bp
  20% of sequence in fragments shorter than    5080 bp
  25% of sequence in fragments shorter than    6303 bp
  30% of sequence in fragments shorter than    7599 bp
  35% of sequence in fragments shorter than    9014 bp
  40% of sequence in fragments shorter than   10548 bp
  45% of sequence in fragments shorter than   12243 bp
  50% of sequence in fragments shorter than   14122 bp
  55% of sequence in fragments shorter than   16260 bp
  60% of sequence in fragments shorter than   18681 bp
  65% of sequence in fragments shorter than   21437 bp
  70% of sequence in fragments shorter than   24707 bp
  75% of sequence in fragments shorter than   28716 bp
  80% of sequence in fragments shorter than   33793 bp
  85% of sequence in fragments shorter than   40394 bp
  90% of sequence in fragments shorter than   50381 bp
  95% of sequence in fragments shorter than   71893 bp

# MfeI.PmeI (CAATTG+GTTTAAAC) double digest, 605 thousand fragments (564303+42094 sites), 4917 bp average fragment length
   5% of sequence in fragments shorter than    1698 bp
  10% of sequence in fragments shorter than    2553 bp
  15% of sequence in fragments shorter than    3319 bp
  20% of sequence in fragments shorter than    4049 bp
  25% of sequence in fragments shorter than    4744 bp
  30% of sequence in fragments shorter than    5452 bp
  35% of sequence in fragments shorter than    6176 bp
  40% of sequence in fragments shorter than    6926 bp
  45% of sequence in fragments shorter than    7725 bp
  50% of sequence in fragments shorter than    8589 bp
  55% of sequence in fragments shorter than    9535 bp
  60% of sequence in fragments shorter than   10592 bp
  65% of sequence in fragments shorter than   11760 bp
  70% of sequence in fragments shorter than   13113 bp
  75% of sequence in fragments shorter than   14756 bp
  80% of sequence in fragments shorter than   16782 bp
  85% of sequence in fragments shorter than   19600 bp
  90% of sequence in fragments shorter than   23998 bp
  95% of sequence in fragments shorter than   33393 bp

# EcoRV.PacI (GATATC+TTAATTAA) double digest, 605 thousand fragments (446473+159455 sites), 4921 bp average fragment length
   5% of sequence in fragments shorter than    1717 bp
  10% of sequence in fragments shorter than    2611 bp
  15% of sequence in fragments shorter than    3401 bp
  20% of sequence in fragments shorter than    4150 bp
  25% of sequence in fragments shorter than    4881 bp
  30% of sequence in fragments shorter than    5618 bp
  35% of sequence in fragments shorter than    6377 bp
  40% of sequence in fragments shorter than    7165 bp
  45% of sequence in fragments shorter than    8009 bp
  50% of sequence in fragments shorter than    8909 bp
  55% of sequence in fragments shorter than    9888 bp
  60% of sequence in fragments shorter than   10993 bp
  65% of sequence in fragments shorter than   12235 bp
  70% of sequence in fragments shorter than   13683 bp
  75% of sequence in fragments shorter than   15446 bp
  80% of sequence in fragments shorter than   17616 bp
  85% of sequence in fragments shorter than   20552 bp
  90% of sequence in fragments shorter than   25231 bp
  95% of sequence in fragments shorter than   34985 bp

# AvrII.NotI (CCTAGG+GCGGCCGC) double digest, 604 thousand fragments (594956+9795 sites), 4930 bp average fragment length
   5% of sequence in fragments shorter than    1716 bp
  10% of sequence in fragments shorter than    2642 bp
  15% of sequence in fragments shorter than    3439 bp
  20% of sequence in fragments shorter than    4178 bp
  25% of sequence in fragments shorter than    4885 bp
  30% of sequence in fragments shorter than    5597 bp
  35% of sequence in fragments shorter than    6325 bp
  40% of sequence in fragments shorter than    7075 bp
  45% of sequence in fragments shorter than    7868 bp
  50% of sequence in fragments shorter than    8696 bp
  55% of sequence in fragments shorter than    9607 bp
  60% of sequence in fragments shorter than   10590 bp
  65% of sequence in fragments shorter than   11688 bp
  70% of sequence in fragments shorter than   12979 bp
  75% of sequence in fragments shorter than   14459 bp
  80% of sequence in fragments shorter than   16286 bp
  85% of sequence in fragments shorter than   18672 bp
  90% of sequence in fragments shorter than   22301 bp
  95% of sequence in fragments shorter than   29721 bp

# AvrII.RsrII (CCTAGG+CGGWCCG) double digest, 604 thousand fragments (594956+9764 sites), 4930 bp average fragment length
   5% of sequence in fragments shorter than    1717 bp
  10% of sequence in fragments shorter than    2641 bp
  15% of sequence in fragments shorter than    3437 bp
  20% of sequence in fragments shorter than    4172 bp
  25% of sequence in fragments shorter than    4880 bp
  30% of sequence in fragments shorter than    5590 bp
  35% of sequence in fragments shorter than    6315 bp
  40% of sequence in fragments shorter than    7066 bp
  45% of sequence in fragments shorter than    7857 bp
  50% of sequence in fragments shorter than    8686 bp
  55% of sequence in fragments shorter than    9595 bp
  60% of sequence in fragments shorter than   10573 bp
  65% of sequence in fragments shorter than   11666 bp
  70% of sequence in fragments shorter than   12959 bp
  75% of sequence in fragments shorter than   14439 bp
  80% of sequence in fragments shorter than   16266 bp
  85% of sequence in fragments shorter than   18635 bp
  90% of sequence in fragments shorter than   22251 bp
  95% of sequence in fragments shorter than   29617 bp

# AgeI.SphI (ACCGGT+GCATGC) double digest, 603 thousand fragments (53386+550952 sites), 4934 bp average fragment length
   5% of sequence in fragments shorter than    1725 bp
  10% of sequence in fragments shorter than    2607 bp
  15% of sequence in fragments shorter than    3384 bp
  20% of sequence in fragments shorter than    4116 bp
  25% of sequence in fragments shorter than    4828 bp
  30% of sequence in fragments shorter than    5541 bp
  35% of sequence in fragments shorter than    6261 bp
  40% of sequence in fragments shorter than    7014 bp
  45% of sequence in fragments shorter than    7799 bp
  50% of sequence in fragments shorter than    8639 bp
  55% of sequence in fragments shorter than    9544 bp
  60% of sequence in fragments shorter than   10552 bp
  65% of sequence in fragments shorter than   11687 bp
  70% of sequence in fragments shorter than   12957 bp
  75% of sequence in fragments shorter than   14458 bp
  80% of sequence in fragments shorter than   16284 bp
  85% of sequence in fragments shorter than   18690 bp
  90% of sequence in fragments shorter than   22273 bp
  95% of sequence in fragments shorter than   29315 bp

# EcoRV.Hpy99I (GATATC+CGWCG) double digest, 603 thousand fragments (446473+157917 sites), 4934 bp average fragment length
   5% of sequence in fragments shorter than    1734 bp
  10% of sequence in fragments shorter than    2694 bp
  15% of sequence in fragments shorter than    3507 bp
  20% of sequence in fragments shorter than    4292 bp
  25% of sequence in fragments shorter than    5042 bp
  30% of sequence in fragments shorter than    5793 bp
  35% of sequence in fragments shorter than    6559 bp
  40% of sequence in fragments shorter than    7350 bp
  45% of sequence in fragments shorter than    8179 bp
  50% of sequence in fragments shorter than    9044 bp
  55% of sequence in fragments shorter than    9972 bp
  60% of sequence in fragments shorter than   10992 bp
  65% of sequence in fragments shorter than   12107 bp
  70% of sequence in fragments shorter than   13375 bp
  75% of sequence in fragments shorter than   14846 bp
  80% of sequence in fragments shorter than   16639 bp
  85% of sequence in fragments shorter than   18956 bp
  90% of sequence in fragments shorter than   22294 bp
  95% of sequence in fragments shorter than   28335 bp

# SapI.SwaI (GCTCTTC+ATTTAAAT) double digest, 603 thousand fragments (377161+226602 sites), 4938 bp average fragment length
   5% of sequence in fragments shorter than    1725 bp
  10% of sequence in fragments shorter than    2623 bp
  15% of sequence in fragments shorter than    3401 bp
  20% of sequence in fragments shorter than    4124 bp
  25% of sequence in fragments shorter than    4818 bp
  30% of sequence in fragments shorter than    5512 bp
  35% of sequence in fragments shorter than    6219 bp
  40% of sequence in fragments shorter than    6962 bp
  45% of sequence in fragments shorter than    7735 bp
  50% of sequence in fragments shorter than    8539 bp
  55% of sequence in fragments shorter than    9404 bp
  60% of sequence in fragments shorter than   10365 bp
  65% of sequence in fragments shorter than   11433 bp
  70% of sequence in fragments shorter than   12667 bp
  75% of sequence in fragments shorter than   14095 bp
  80% of sequence in fragments shorter than   15841 bp
  85% of sequence in fragments shorter than   18092 bp
  90% of sequence in fragments shorter than   21519 bp
  95% of sequence in fragments shorter than   28699 bp

# SmaI.SwaI (CCCGGG+ATTTAAAT) double digest, 603 thousand fragments (376940+226602 sites), 4939 bp average fragment length
   5% of sequence in fragments shorter than    1668 bp
  10% of sequence in fragments shorter than    2623 bp
  15% of sequence in fragments shorter than    3467 bp
  20% of sequence in fragments shorter than    4281 bp
  25% of sequence in fragments shorter than    5097 bp
  30% of sequence in fragments shorter than    5925 bp
  35% of sequence in fragments shorter than    6790 bp
  40% of sequence in fragments shorter than    7689 bp
  45% of sequence in fragments shorter than    8638 bp
  50% of sequence in fragments shorter than    9647 bp
  55% of sequence in fragments shorter than   10756 bp
  60% of sequence in fragments shorter than   11968 bp
  65% of sequence in fragments shorter than   13341 bp
  70% of sequence in fragments shorter than   14902 bp
  75% of sequence in fragments shorter than   16789 bp
  80% of sequence in fragments shorter than   19093 bp
  85% of sequence in fragments shorter than   22152 bp
  90% of sequence in fragments shorter than   26722 bp
  95% of sequence in fragments shorter than   36663 bp

# HgaI.SalI (GACGC+GTCGAC) double digest, 602 thousand fragments (571889+31396 sites), 4942 bp average fragment length
   5% of sequence in fragments shorter than    1659 bp
  10% of sequence in fragments shorter than    2769 bp
  15% of sequence in fragments shorter than    3784 bp
  20% of sequence in fragments shorter than    4802 bp
  25% of sequence in fragments shorter than    5830 bp
  30% of sequence in fragments shorter than    6871 bp
  35% of sequence in fragments shorter than    7959 bp
  40% of sequence in fragments shorter than    9127 bp
  45% of sequence in fragments shorter than   10369 bp
  50% of sequence in fragments shorter than   11722 bp
  55% of sequence in fragments shorter than   13225 bp
  60% of sequence in fragments shorter than   14877 bp
  65% of sequence in fragments shorter than   16695 bp
  70% of sequence in fragments shorter than   18794 bp
  75% of sequence in fragments shorter than   21302 bp
  80% of sequence in fragments shorter than   24355 bp
  85% of sequence in fragments shorter than   28387 bp
  90% of sequence in fragments shorter than   34410 bp
  95% of sequence in fragments shorter than   45720 bp

# PspXI.ScaI (VCTCGAGB+AGTACT) double digest, 602 thousand fragments (59193+543793 sites), 4945 bp average fragment length
   5% of sequence in fragments shorter than    1718 bp
  10% of sequence in fragments shorter than    2595 bp
  15% of sequence in fragments shorter than    3362 bp
  20% of sequence in fragments shorter than    4085 bp
  25% of sequence in fragments shorter than    4793 bp
  30% of sequence in fragments shorter than    5504 bp
  35% of sequence in fragments shorter than    6232 bp
  40% of sequence in fragments shorter than    6990 bp
  45% of sequence in fragments shorter than    7785 bp
  50% of sequence in fragments shorter than    8616 bp
  55% of sequence in fragments shorter than    9502 bp
  60% of sequence in fragments shorter than   10479 bp
  65% of sequence in fragments shorter than   11585 bp
  70% of sequence in fragments shorter than   12839 bp
  75% of sequence in fragments shorter than   14303 bp
  80% of sequence in fragments shorter than   16079 bp
  85% of sequence in fragments shorter than   18396 bp
  90% of sequence in fragments shorter than   21848 bp
  95% of sequence in fragments shorter than   28823 bp

# EciI.SalI (GGCGGA+GTCGAC) double digest, 602 thousand fragments (571273+31396 sites), 4947 bp average fragment length
   5% of sequence in fragments shorter than    1595 bp
  10% of sequence in fragments shorter than    2622 bp
  15% of sequence in fragments shorter than    3584 bp
  20% of sequence in fragments shorter than    4561 bp
  25% of sequence in fragments shorter than    5584 bp
  30% of sequence in fragments shorter than    6669 bp
  35% of sequence in fragments shorter than    7824 bp
  40% of sequence in fragments shorter than    9064 bp
  45% of sequence in fragments shorter than   10460 bp
  50% of sequence in fragments shorter than   11994 bp
  55% of sequence in fragments shorter than   13716 bp
  60% of sequence in fragments shorter than   15689 bp
  65% of sequence in fragments shorter than   17888 bp
  70% of sequence in fragments shorter than   20494 bp
  75% of sequence in fragments shorter than   23583 bp
  80% of sequence in fragments shorter than   27448 bp
  85% of sequence in fragments shorter than   32777 bp
  90% of sequence in fragments shorter than   40602 bp
  95% of sequence in fragments shorter than   56479 bp

# BamHI.BsrBI (GGATCC+CCGCTC) double digest, 601 thousand fragments (365999+235523 sites), 4957 bp average fragment length
   5% of sequence in fragments shorter than    1686 bp
  10% of sequence in fragments shorter than    2625 bp
  15% of sequence in fragments shorter than    3526 bp
  20% of sequence in fragments shorter than    4391 bp
  25% of sequence in fragments shorter than    5244 bp
  30% of sequence in fragments shorter than    6122 bp
  35% of sequence in fragments shorter than    7025 bp
  40% of sequence in fragments shorter than    7969 bp
  45% of sequence in fragments shorter than    8957 bp
  50% of sequence in fragments shorter than   10034 bp
  55% of sequence in fragments shorter than   11176 bp
  60% of sequence in fragments shorter than   12450 bp
  65% of sequence in fragments shorter than   13889 bp
  70% of sequence in fragments shorter than   15530 bp
  75% of sequence in fragments shorter than   17480 bp
  80% of sequence in fragments shorter than   19891 bp
  85% of sequence in fragments shorter than   23034 bp
  90% of sequence in fragments shorter than   27512 bp
  95% of sequence in fragments shorter than   36126 bp

# AscI.AvrII (GGCGCGCC+CCTAGG) double digest, 599 thousand fragments (4751+594956 sites), 4971 bp average fragment length
   5% of sequence in fragments shorter than    1732 bp
  10% of sequence in fragments shorter than    2664 bp
  15% of sequence in fragments shorter than    3466 bp
  20% of sequence in fragments shorter than    4205 bp
  25% of sequence in fragments shorter than    4919 bp
  30% of sequence in fragments shorter than    5635 bp
  35% of sequence in fragments shorter than    6366 bp
  40% of sequence in fragments shorter than    7120 bp
  45% of sequence in fragments shorter than    7915 bp
  50% of sequence in fragments shorter than    8747 bp
  55% of sequence in fragments shorter than    9657 bp
  60% of sequence in fragments shorter than   10646 bp
  65% of sequence in fragments shorter than   11750 bp
  70% of sequence in fragments shorter than   13045 bp
  75% of sequence in fragments shorter than   14537 bp
  80% of sequence in fragments shorter than   16372 bp
  85% of sequence in fragments shorter than   18754 bp
  90% of sequence in fragments shorter than   22398 bp
  95% of sequence in fragments shorter than   29817 bp

# AgeI.ScaI (ACCGGT+AGTACT) double digest, 596 thousand fragments (53386+543793 sites), 4993 bp average fragment length
   5% of sequence in fragments shorter than    1735 bp
  10% of sequence in fragments shorter than    2620 bp
  15% of sequence in fragments shorter than    3390 bp
  20% of sequence in fragments shorter than    4123 bp
  25% of sequence in fragments shorter than    4830 bp
  30% of sequence in fragments shorter than    5544 bp
  35% of sequence in fragments shorter than    6274 bp
  40% of sequence in fragments shorter than    7029 bp
  45% of sequence in fragments shorter than    7824 bp
  50% of sequence in fragments shorter than    8663 bp
  55% of sequence in fragments shorter than    9568 bp
  60% of sequence in fragments shorter than   10565 bp
  65% of sequence in fragments shorter than   11692 bp
  70% of sequence in fragments shorter than   12982 bp
  75% of sequence in fragments shorter than   14493 bp
  80% of sequence in fragments shorter than   16353 bp
  85% of sequence in fragments shorter than   18785 bp
  90% of sequence in fragments shorter than   22533 bp
  95% of sequence in fragments shorter than   30064 bp

# BamHI.KasI (GGATCC+GGCGCC) double digest, 596 thousand fragments (365999+230381 sites), 4999 bp average fragment length
   5% of sequence in fragments shorter than    1714 bp
  10% of sequence in fragments shorter than    2659 bp
  15% of sequence in fragments shorter than    3522 bp
  20% of sequence in fragments shorter than    4337 bp
  25% of sequence in fragments shorter than    5090 bp
  30% of sequence in fragments shorter than    5913 bp
  35% of sequence in fragments shorter than    6755 bp
  40% of sequence in fragments shorter than    7654 bp
  45% of sequence in fragments shorter than    8588 bp
  50% of sequence in fragments shorter than    9618 bp
  55% of sequence in fragments shorter than   10725 bp
  60% of sequence in fragments shorter than   11957 bp
  65% of sequence in fragments shorter than   13327 bp
  70% of sequence in fragments shorter than   14917 bp
  75% of sequence in fragments shorter than   16796 bp
  80% of sequence in fragments shorter than   19094 bp
  85% of sequence in fragments shorter than   22140 bp
  90% of sequence in fragments shorter than   26653 bp
  95% of sequence in fragments shorter than   35617 bp

# MfeI.SalI (CAATTG+GTCGAC) double digest, 595 thousand fragments (564303+31396 sites), 5006 bp average fragment length
   5% of sequence in fragments shorter than    1737 bp
  10% of sequence in fragments shorter than    2612 bp
  15% of sequence in fragments shorter than    3391 bp
  20% of sequence in fragments shorter than    4126 bp
  25% of sequence in fragments shorter than    4831 bp
  30% of sequence in fragments shorter than    5546 bp
  35% of sequence in fragments shorter than    6277 bp
  40% of sequence in fragments shorter than    7040 bp
  45% of sequence in fragments shorter than    7840 bp
  50% of sequence in fragments shorter than    8713 bp
  55% of sequence in fragments shorter than    9658 bp
  60% of sequence in fragments shorter than   10706 bp
  65% of sequence in fragments shorter than   11853 bp
  70% of sequence in fragments shorter than   13194 bp
  75% of sequence in fragments shorter than   14793 bp
  80% of sequence in fragments shorter than   16765 bp
  85% of sequence in fragments shorter than   19467 bp
  90% of sequence in fragments shorter than   23575 bp
  95% of sequence in fragments shorter than   31865 bp

# HgaI.MluI (GACGC+ACGCGT) double digest, 592 thousand fragments (571889+21415 sites), 5026 bp average fragment length
   5% of sequence in fragments shorter than    1705 bp
  10% of sequence in fragments shorter than    2847 bp
  15% of sequence in fragments shorter than    3915 bp
  20% of sequence in fragments shorter than    4978 bp
  25% of sequence in fragments shorter than    6043 bp
  30% of sequence in fragments shorter than    7143 bp
  35% of sequence in fragments shorter than    8298 bp
  40% of sequence in fragments shorter than    9520 bp
  45% of sequence in fragments shorter than   10837 bp
  50% of sequence in fragments shorter than   12272 bp
  55% of sequence in fragments shorter than   13865 bp
  60% of sequence in fragments shorter than   15610 bp
  65% of sequence in fragments shorter than   17521 bp
  70% of sequence in fragments shorter than   19782 bp
  75% of sequence in fragments shorter than   22416 bp
  80% of sequence in fragments shorter than   25703 bp
  85% of sequence in fragments shorter than   29939 bp
  90% of sequence in fragments shorter than   36222 bp
  95% of sequence in fragments shorter than   48261 bp

# PmeI.SphI (GTTTAAAC+GCATGC) double digest, 592 thousand fragments (42094+550952 sites), 5028 bp average fragment length
   5% of sequence in fragments shorter than    1756 bp
  10% of sequence in fragments shorter than    2653 bp
  15% of sequence in fragments shorter than    3441 bp
  20% of sequence in fragments shorter than    4185 bp
  25% of sequence in fragments shorter than    4912 bp
  30% of sequence in fragments shorter than    5645 bp
  35% of sequence in fragments shorter than    6386 bp
  40% of sequence in fragments shorter than    7149 bp
  45% of sequence in fragments shorter than    7948 bp
  50% of sequence in fragments shorter than    8797 bp
  55% of sequence in fragments shorter than    9723 bp
  60% of sequence in fragments shorter than   10747 bp
  65% of sequence in fragments shorter than   11876 bp
  70% of sequence in fragments shorter than   13174 bp
  75% of sequence in fragments shorter than   14682 bp
  80% of sequence in fragments shorter than   16545 bp
  85% of sequence in fragments shorter than   18938 bp
  90% of sequence in fragments shorter than   22448 bp
  95% of sequence in fragments shorter than   29360 bp

# EciI.MluI (GGCGGA+ACGCGT) double digest, 592 thousand fragments (571273+21415 sites), 5030 bp average fragment length
   5% of sequence in fragments shorter than    1616 bp
  10% of sequence in fragments shorter than    2667 bp
  15% of sequence in fragments shorter than    3654 bp
  20% of sequence in fragments shorter than    4668 bp
  25% of sequence in fragments shorter than    5734 bp
  30% of sequence in fragments shorter than    6870 bp
  35% of sequence in fragments shorter than    8083 bp
  40% of sequence in fragments shorter than    9405 bp
  45% of sequence in fragments shorter than   10882 bp
  50% of sequence in fragments shorter than   12508 bp
  55% of sequence in fragments shorter than   14344 bp
  60% of sequence in fragments shorter than   16458 bp
  65% of sequence in fragments shorter than   18838 bp
  70% of sequence in fragments shorter than   21630 bp
  75% of sequence in fragments shorter than   25015 bp
  80% of sequence in fragments shorter than   29204 bp
  85% of sequence in fragments shorter than   34871 bp
  90% of sequence in fragments shorter than   43282 bp
  95% of sequence in fragments shorter than   61117 bp

# ApaI.SnaBI (GGGCCC+TACGTA) double digest, 592 thousand fragments (460339+132299 sites), 5031 bp average fragment length
   5% of sequence in fragments shorter than    1631 bp
  10% of sequence in fragments shorter than    2710 bp
  15% of sequence in fragments shorter than    3699 bp
  20% of sequence in fragments shorter than    4686 bp
  25% of sequence in fragments shorter than    5689 bp
  30% of sequence in fragments shorter than    6704 bp
  35% of sequence in fragments shorter than    7750 bp
  40% of sequence in fragments shorter than    8850 bp
  45% of sequence in fragments shorter than   10011 bp
  50% of sequence in fragments shorter than   11239 bp
  55% of sequence in fragments shorter than   12604 bp
  60% of sequence in fragments shorter than   14098 bp
  65% of sequence in fragments shorter than   15781 bp
  70% of sequence in fragments shorter than   17649 bp
  75% of sequence in fragments shorter than   19833 bp
  80% of sequence in fragments shorter than   22654 bp
  85% of sequence in fragments shorter than   26260 bp
  90% of sequence in fragments shorter than   31624 bp
  95% of sequence in fragments shorter than   42357 bp

# BamHI.SwaI (GGATCC+ATTTAAAT) double digest, 592 thousand fragments (365999+226602 sites), 5031 bp average fragment length
   5% of sequence in fragments shorter than    1791 bp
  10% of sequence in fragments shorter than    2674 bp
  15% of sequence in fragments shorter than    3449 bp
  20% of sequence in fragments shorter than    4183 bp
  25% of sequence in fragments shorter than    4892 bp
  30% of sequence in fragments shorter than    5585 bp
  35% of sequence in fragments shorter than    6295 bp
  40% of sequence in fragments shorter than    7031 bp
  45% of sequence in fragments shorter than    7806 bp
  50% of sequence in fragments shorter than    8606 bp
  55% of sequence in fragments shorter than    9476 bp
  60% of sequence in fragments shorter than   10428 bp
  65% of sequence in fragments shorter than   11485 bp
  70% of sequence in fragments shorter than   12682 bp
  75% of sequence in fragments shorter than   14073 bp
  80% of sequence in fragments shorter than   15763 bp
  85% of sequence in fragments shorter than   17932 bp
  90% of sequence in fragments shorter than   21153 bp
  95% of sequence in fragments shorter than   27571 bp

# HgaI.SgrAI (GACGC+CRCCGGYG) double digest, 586 thousand fragments (571889+15270 sites), 5078 bp average fragment length
   5% of sequence in fragments shorter than    1709 bp
  10% of sequence in fragments shorter than    2861 bp
  15% of sequence in fragments shorter than    3939 bp
  20% of sequence in fragments shorter than    5014 bp
  25% of sequence in fragments shorter than    6088 bp
  30% of sequence in fragments shorter than    7198 bp
  35% of sequence in fragments shorter than    8365 bp
  40% of sequence in fragments shorter than    9596 bp
  45% of sequence in fragments shorter than   10927 bp
  50% of sequence in fragments shorter than   12374 bp
  55% of sequence in fragments shorter than   13978 bp
  60% of sequence in fragments shorter than   15733 bp
  65% of sequence in fragments shorter than   17658 bp
  70% of sequence in fragments shorter than   19957 bp
  75% of sequence in fragments shorter than   22610 bp
  80% of sequence in fragments shorter than   25921 bp
  85% of sequence in fragments shorter than   30249 bp
  90% of sequence in fragments shorter than   36632 bp
  95% of sequence in fragments shorter than   48963 bp

# HgaI.NruI (GACGC+TCGCGA) double digest, 586 thousand fragments (571889+14872 sites), 5082 bp average fragment length
   5% of sequence in fragments shorter than    1706 bp
  10% of sequence in fragments shorter than    2845 bp
  15% of sequence in fragments shorter than    3911 bp
  20% of sequence in fragments shorter than    4975 bp
  25% of sequence in fragments shorter than    6037 bp
  30% of sequence in fragments shorter than    7137 bp
  35% of sequence in fragments shorter than    8289 bp
  40% of sequence in fragments shorter than    9513 bp
  45% of sequence in fragments shorter than   10830 bp
  50% of sequence in fragments shorter than   12259 bp
  55% of sequence in fragments shorter than   13848 bp
  60% of sequence in fragments shorter than   15578 bp
  65% of sequence in fragments shorter than   17499 bp
  70% of sequence in fragments shorter than   19759 bp
  75% of sequence in fragments shorter than   22374 bp
  80% of sequence in fragments shorter than   25618 bp
  85% of sequence in fragments shorter than   29913 bp
  90% of sequence in fragments shorter than   36221 bp
  95% of sequence in fragments shorter than   48350 bp

# EciI.SgrAI (GGCGGA+CRCCGGYG) double digest, 586 thousand fragments (571273+15270 sites), 5083 bp average fragment length
   5% of sequence in fragments shorter than    1638 bp
  10% of sequence in fragments shorter than    2706 bp
  15% of sequence in fragments shorter than    3712 bp
  20% of sequence in fragments shorter than    4751 bp
  25% of sequence in fragments shorter than    5839 bp
  30% of sequence in fragments shorter than    6995 bp
  35% of sequence in fragments shorter than    8232 bp
  40% of sequence in fragments shorter than    9581 bp
  45% of sequence in fragments shorter than   11088 bp
  50% of sequence in fragments shorter than   12740 bp
  55% of sequence in fragments shorter than   14627 bp
  60% of sequence in fragments shorter than   16756 bp
  65% of sequence in fragments shorter than   19174 bp
  70% of sequence in fragments shorter than   22013 bp
  75% of sequence in fragments shorter than   25473 bp
  80% of sequence in fragments shorter than   29681 bp
  85% of sequence in fragments shorter than   35408 bp
  90% of sequence in fragments shorter than   44110 bp
  95% of sequence in fragments shorter than   62507 bp

# EciI.NruI (GGCGGA+TCGCGA) double digest, 585 thousand fragments (571273+14872 sites), 5086 bp average fragment length
   5% of sequence in fragments shorter than    1639 bp
  10% of sequence in fragments shorter than    2706 bp
  15% of sequence in fragments shorter than    3713 bp
  20% of sequence in fragments shorter than    4746 bp
  25% of sequence in fragments shorter than    5829 bp
  30% of sequence in fragments shorter than    6984 bp
  35% of sequence in fragments shorter than    8219 bp
  40% of sequence in fragments shorter than    9565 bp
  45% of sequence in fragments shorter than   11068 bp
  50% of sequence in fragments shorter than   12722 bp
  55% of sequence in fragments shorter than   14596 bp
  60% of sequence in fragments shorter than   16710 bp
  65% of sequence in fragments shorter than   19135 bp
  70% of sequence in fragments shorter than   21945 bp
  75% of sequence in fragments shorter than   25385 bp
  80% of sequence in fragments shorter than   29585 bp
  85% of sequence in fragments shorter than   35262 bp
  90% of sequence in fragments shorter than   43864 bp
  95% of sequence in fragments shorter than   61667 bp

# ApaI.NaeI (GGGCCC+GCCGGC) double digest, 585 thousand fragments (460339+125692 sites), 5087 bp average fragment length
   5% of sequence in fragments shorter than    1606 bp
  10% of sequence in fragments shorter than    2867 bp
  15% of sequence in fragments shorter than    4114 bp
  20% of sequence in fragments shorter than    5388 bp
  25% of sequence in fragments shorter than    6729 bp
  30% of sequence in fragments shorter than    8121 bp
  35% of sequence in fragments shorter than    9591 bp
  40% of sequence in fragments shorter than   11139 bp
  45% of sequence in fragments shorter than   12802 bp
  50% of sequence in fragments shorter than   14658 bp
  55% of sequence in fragments shorter than   16662 bp
  60% of sequence in fragments shorter than   18937 bp
  65% of sequence in fragments shorter than   21552 bp
  70% of sequence in fragments shorter than   24520 bp
  75% of sequence in fragments shorter than   28122 bp
  80% of sequence in fragments shorter than   32660 bp
  85% of sequence in fragments shorter than   38813 bp
  90% of sequence in fragments shorter than   48151 bp
  95% of sequence in fragments shorter than   67561 bp

# HgaI.PvuI (GACGC+CGATCG) double digest, 585 thousand fragments (571889+14186 sites), 5088 bp average fragment length
   5% of sequence in fragments shorter than    1709 bp
  10% of sequence in fragments shorter than    2849 bp
  15% of sequence in fragments shorter than    3921 bp
  20% of sequence in fragments shorter than    4987 bp
  25% of sequence in fragments shorter than    6054 bp
  30% of sequence in fragments shorter than    7153 bp
  35% of sequence in fragments shorter than    8305 bp
  40% of sequence in fragments shorter than    9524 bp
  45% of sequence in fragments shorter than   10842 bp
  50% of sequence in fragments shorter than   12273 bp
  55% of sequence in fragments shorter than   13864 bp
  60% of sequence in fragments shorter than   15601 bp
  65% of sequence in fragments shorter than   17517 bp
  70% of sequence in fragments shorter than   19774 bp
  75% of sequence in fragments shorter than   22403 bp
  80% of sequence in fragments shorter than   25668 bp
  85% of sequence in fragments shorter than   29890 bp
  90% of sequence in fragments shorter than   36181 bp
  95% of sequence in fragments shorter than   48077 bp

# KpnI.SexAI (GGTACC+ACCWGGT) double digest, 585 thousand fragments (287974+297900 sites), 5089 bp average fragment length
   5% of sequence in fragments shorter than    1726 bp
  10% of sequence in fragments shorter than    2670 bp
  15% of sequence in fragments shorter than    3482 bp
  20% of sequence in fragments shorter than    4253 bp
  25% of sequence in fragments shorter than    5014 bp
  30% of sequence in fragments shorter than    5781 bp
  35% of sequence in fragments shorter than    6555 bp
  40% of sequence in fragments shorter than    7360 bp
  45% of sequence in fragments shorter than    8200 bp
  50% of sequence in fragments shorter than    9089 bp
  55% of sequence in fragments shorter than   10044 bp
  60% of sequence in fragments shorter than   11089 bp
  65% of sequence in fragments shorter than   12247 bp
  70% of sequence in fragments shorter than   13564 bp
  75% of sequence in fragments shorter than   15105 bp
  80% of sequence in fragments shorter than   17024 bp
  85% of sequence in fragments shorter than   19512 bp
  90% of sequence in fragments shorter than   23219 bp
  95% of sequence in fragments shorter than   30640 bp

# PmeI.ScaI (GTTTAAAC+AGTACT) double digest, 585 thousand fragments (42094+543793 sites), 5089 bp average fragment length
   5% of sequence in fragments shorter than    1747 bp
  10% of sequence in fragments shorter than    2646 bp
  15% of sequence in fragments shorter than    3443 bp
  20% of sequence in fragments shorter than    4199 bp
  25% of sequence in fragments shorter than    4935 bp
  30% of sequence in fragments shorter than    5686 bp
  35% of sequence in fragments shorter than    6451 bp
  40% of sequence in fragments shorter than    7251 bp
  45% of sequence in fragments shorter than    8083 bp
  50% of sequence in fragments shorter than    8972 bp
  55% of sequence in fragments shorter than    9929 bp
  60% of sequence in fragments shorter than   11005 bp
  65% of sequence in fragments shorter than   12217 bp
  70% of sequence in fragments shorter than   13615 bp
  75% of sequence in fragments shorter than   15244 bp
  80% of sequence in fragments shorter than   17289 bp
  85% of sequence in fragments shorter than   20068 bp
  90% of sequence in fragments shorter than   24351 bp
  95% of sequence in fragments shorter than   33735 bp

# MfeI.MluI (CAATTG+ACGCGT) double digest, 585 thousand fragments (564303+21415 sites), 5091 bp average fragment length
   5% of sequence in fragments shorter than    1771 bp
  10% of sequence in fragments shorter than    2661 bp
  15% of sequence in fragments shorter than    3452 bp
  20% of sequence in fragments shorter than    4207 bp
  25% of sequence in fragments shorter than    4926 bp
  30% of sequence in fragments shorter than    5650 bp
  35% of sequence in fragments shorter than    6391 bp
  40% of sequence in fragments shorter than    7160 bp
  45% of sequence in fragments shorter than    7979 bp
  50% of sequence in fragments shorter than    8856 bp
  55% of sequence in fragments shorter than    9821 bp
  60% of sequence in fragments shorter than   10874 bp
  65% of sequence in fragments shorter than   12045 bp
  70% of sequence in fragments shorter than   13377 bp
  75% of sequence in fragments shorter than   14993 bp
  80% of sequence in fragments shorter than   16984 bp
  85% of sequence in fragments shorter than   19657 bp
  90% of sequence in fragments shorter than   23693 bp
  95% of sequence in fragments shorter than   31667 bp

# FseI.HgaI (GGCCGGCC+GACGC) double digest, 585 thousand fragments (13662+571889 sites), 5092 bp average fragment length
   5% of sequence in fragments shorter than    1705 bp
  10% of sequence in fragments shorter than    2857 bp
  15% of sequence in fragments shorter than    3936 bp
  20% of sequence in fragments shorter than    5014 bp
  25% of sequence in fragments shorter than    6096 bp
  30% of sequence in fragments shorter than    7208 bp
  35% of sequence in fragments shorter than    8378 bp
  40% of sequence in fragments shorter than    9614 bp
  45% of sequence in fragments shorter than   10956 bp
  50% of sequence in fragments shorter than   12401 bp
  55% of sequence in fragments shorter than   14018 bp
  60% of sequence in fragments shorter than   15777 bp
  65% of sequence in fragments shorter than   17710 bp
  70% of sequence in fragments shorter than   20004 bp
  75% of sequence in fragments shorter than   22674 bp
  80% of sequence in fragments shorter than   26003 bp
  85% of sequence in fragments shorter than   30347 bp
  90% of sequence in fragments shorter than   36717 bp
  95% of sequence in fragments shorter than   49184 bp

# EciI.PvuI (GGCGGA+CGATCG) double digest, 585 thousand fragments (571273+14186 sites), 5092 bp average fragment length
   5% of sequence in fragments shorter than    1641 bp
  10% of sequence in fragments shorter than    2693 bp
  15% of sequence in fragments shorter than    3693 bp
  20% of sequence in fragments shorter than    4723 bp
  25% of sequence in fragments shorter than    5804 bp
  30% of sequence in fragments shorter than    6950 bp
  35% of sequence in fragments shorter than    8179 bp
  40% of sequence in fragments shorter than    9515 bp
  45% of sequence in fragments shorter than   11006 bp
  50% of sequence in fragments shorter than   12653 bp
  55% of sequence in fragments shorter than   14507 bp
  60% of sequence in fragments shorter than   16610 bp
  65% of sequence in fragments shorter than   19010 bp
  70% of sequence in fragments shorter than   21797 bp
  75% of sequence in fragments shorter than   25212 bp
  80% of sequence in fragments shorter than   29375 bp
  85% of sequence in fragments shorter than   34982 bp
  90% of sequence in fragments shorter than   43264 bp
  95% of sequence in fragments shorter than   60298 bp

# NheI.SexAI (GCTAGC+ACCWGGT) double digest, 584 thousand fragments (287384+297900 sites), 5094 bp average fragment length
   5% of sequence in fragments shorter than    1762 bp
  10% of sequence in fragments shorter than    2638 bp
  15% of sequence in fragments shorter than    3407 bp
  20% of sequence in fragments shorter than    4184 bp
  25% of sequence in fragments shorter than    4943 bp
  30% of sequence in fragments shorter than    5702 bp
  35% of sequence in fragments shorter than    6474 bp
  40% of sequence in fragments shorter than    7264 bp
  45% of sequence in fragments shorter than    8083 bp
  50% of sequence in fragments shorter than    8953 bp
  55% of sequence in fragments shorter than    9891 bp
  60% of sequence in fragments shorter than   10914 bp
  65% of sequence in fragments shorter than   12064 bp
  70% of sequence in fragments shorter than   13345 bp
  75% of sequence in fragments shorter than   14857 bp
  80% of sequence in fragments shorter than   16684 bp
  85% of sequence in fragments shorter than   19100 bp
  90% of sequence in fragments shorter than   22552 bp
  95% of sequence in fragments shorter than   29553 bp

# EciI.FseI (GGCGGA+GGCCGGCC) double digest, 584 thousand fragments (571273+13662 sites), 5097 bp average fragment length
   5% of sequence in fragments shorter than    1637 bp
  10% of sequence in fragments shorter than    2704 bp
  15% of sequence in fragments shorter than    3712 bp
  20% of sequence in fragments shorter than    4752 bp
  25% of sequence in fragments shorter than    5841 bp
  30% of sequence in fragments shorter than    7002 bp
  35% of sequence in fragments shorter than    8247 bp
  40% of sequence in fragments shorter than    9617 bp
  45% of sequence in fragments shorter than   11136 bp
  50% of sequence in fragments shorter than   12811 bp
  55% of sequence in fragments shorter than   14725 bp
  60% of sequence in fragments shorter than   16880 bp
  65% of sequence in fragments shorter than   19315 bp
  70% of sequence in fragments shorter than   22176 bp
  75% of sequence in fragments shorter than   25641 bp
  80% of sequence in fragments shorter than   29935 bp
  85% of sequence in fragments shorter than   35696 bp
  90% of sequence in fragments shorter than   44537 bp
  95% of sequence in fragments shorter than   63083 bp

# ApaLI.ClaI (GTGCAC+ATCGAT) double digest, 582 thousand fragments (496255+86477 sites), 5117 bp average fragment length
   5% of sequence in fragments shorter than    1819 bp
  10% of sequence in fragments shorter than    2721 bp
  15% of sequence in fragments shorter than    3522 bp
  20% of sequence in fragments shorter than    4280 bp
  25% of sequence in fragments shorter than    5020 bp
  30% of sequence in fragments shorter than    5764 bp
  35% of sequence in fragments shorter than    6497 bp
  40% of sequence in fragments shorter than    7274 bp
  45% of sequence in fragments shorter than    8100 bp
  50% of sequence in fragments shorter than    8967 bp
  55% of sequence in fragments shorter than    9895 bp
  60% of sequence in fragments shorter than   10937 bp
  65% of sequence in fragments shorter than   12068 bp
  70% of sequence in fragments shorter than   13388 bp
  75% of sequence in fragments shorter than   14921 bp
  80% of sequence in fragments shorter than   16761 bp
  85% of sequence in fragments shorter than   19186 bp
  90% of sequence in fragments shorter than   22799 bp
  95% of sequence in fragments shorter than   29555 bp

# SalI.SphI (GTCGAC+GCATGC) double digest, 581 thousand fragments (31396+550952 sites), 5120 bp average fragment length
   5% of sequence in fragments shorter than    1787 bp
  10% of sequence in fragments shorter than    2700 bp
  15% of sequence in fragments shorter than    3504 bp
  20% of sequence in fragments shorter than    4264 bp
  25% of sequence in fragments shorter than    5015 bp
  30% of sequence in fragments shorter than    5763 bp
  35% of sequence in fragments shorter than    6529 bp
  40% of sequence in fragments shorter than    7310 bp
  45% of sequence in fragments shorter than    8133 bp
  50% of sequence in fragments shorter than    9005 bp
  55% of sequence in fragments shorter than    9958 bp
  60% of sequence in fragments shorter than   11024 bp
  65% of sequence in fragments shorter than   12192 bp
  70% of sequence in fragments shorter than   13524 bp
  75% of sequence in fragments shorter than   15099 bp
  80% of sequence in fragments shorter than   17023 bp
  85% of sequence in fragments shorter than   19546 bp
  90% of sequence in fragments shorter than   23258 bp
  95% of sequence in fragments shorter than   30500 bp

# ApaI.XhoI (GGGCCC+CTCGAG) double digest, 581 thousand fragments (460339+121308 sites), 5126 bp average fragment length
   5% of sequence in fragments shorter than    1604 bp
  10% of sequence in fragments shorter than    2751 bp
  15% of sequence in fragments shorter than    3855 bp
  20% of sequence in fragments shorter than    4963 bp
  25% of sequence in fragments shorter than    6084 bp
  30% of sequence in fragments shorter than    7248 bp
  35% of sequence in fragments shorter than    8444 bp
  40% of sequence in fragments shorter than    9735 bp
  45% of sequence in fragments shorter than   11112 bp
  50% of sequence in fragments shorter than   12611 bp
  55% of sequence in fragments shorter than   14272 bp
  60% of sequence in fragments shorter than   16098 bp
  65% of sequence in fragments shorter than   18164 bp
  70% of sequence in fragments shorter than   20626 bp
  75% of sequence in fragments shorter than   23483 bp
  80% of sequence in fragments shorter than   27108 bp
  85% of sequence in fragments shorter than   31864 bp
  90% of sequence in fragments shorter than   39330 bp
  95% of sequence in fragments shorter than   55436 bp

# HgaI.NotI (GACGC+GCGGCCGC) double digest, 581 thousand fragments (571889+9795 sites), 5126 bp average fragment length
   5% of sequence in fragments shorter than    1727 bp
  10% of sequence in fragments shorter than    2896 bp
  15% of sequence in fragments shorter than    3983 bp
  20% of sequence in fragments shorter than    5072 bp
  25% of sequence in fragments shorter than    6161 bp
  30% of sequence in fragments shorter than    7277 bp
  35% of sequence in fragments shorter than    8455 bp
  40% of sequence in fragments shorter than    9702 bp
  45% of sequence in fragments shorter than   11051 bp
  50% of sequence in fragments shorter than   12514 bp
  55% of sequence in fragments shorter than   14144 bp
  60% of sequence in fragments shorter than   15902 bp
  65% of sequence in fragments shorter than   17847 bp
  70% of sequence in fragments shorter than   20163 bp
  75% of sequence in fragments shorter than   22821 bp
  80% of sequence in fragments shorter than   26175 bp
  85% of sequence in fragments shorter than   30570 bp
  90% of sequence in fragments shorter than   36959 bp
  95% of sequence in fragments shorter than   49610 bp

# HgaI.RsrII (GACGC+CGGWCCG) double digest, 581 thousand fragments (571889+9764 sites), 5126 bp average fragment length
   5% of sequence in fragments shorter than    1722 bp
  10% of sequence in fragments shorter than    2883 bp
  15% of sequence in fragments shorter than    3967 bp
  20% of sequence in fragments shorter than    5048 bp
  25% of sequence in fragments shorter than    6131 bp
  30% of sequence in fragments shorter than    7243 bp
  35% of sequence in fragments shorter than    8414 bp
  40% of sequence in fragments shorter than    9658 bp
  45% of sequence in fragments shorter than   11004 bp
  50% of sequence in fragments shorter than   12454 bp
  55% of sequence in fragments shorter than   14081 bp
  60% of sequence in fragments shorter than   15844 bp
  65% of sequence in fragments shorter than   17777 bp
  70% of sequence in fragments shorter than   20078 bp
  75% of sequence in fragments shorter than   22747 bp
  80% of sequence in fragments shorter than   26083 bp
  85% of sequence in fragments shorter than   30452 bp
  90% of sequence in fragments shorter than   36831 bp
  95% of sequence in fragments shorter than   49220 bp

# EciI.NotI (GGCGGA+GCGGCCGC) double digest, 580 thousand fragments (571273+9795 sites), 5131 bp average fragment length
   5% of sequence in fragments shorter than    1655 bp
  10% of sequence in fragments shorter than    2738 bp
  15% of sequence in fragments shorter than    3758 bp
  20% of sequence in fragments shorter than    4812 bp
  25% of sequence in fragments shorter than    5913 bp
  30% of sequence in fragments shorter than    7081 bp
  35% of sequence in fragments shorter than    8339 bp
  40% of sequence in fragments shorter than    9726 bp
  45% of sequence in fragments shorter than   11255 bp
  50% of sequence in fragments shorter than   12950 bp
  55% of sequence in fragments shorter than   14873 bp
  60% of sequence in fragments shorter than   17045 bp
  65% of sequence in fragments shorter than   19503 bp
  70% of sequence in fragments shorter than   22398 bp
  75% of sequence in fragments shorter than   25890 bp
  80% of sequence in fragments shorter than   30209 bp
  85% of sequence in fragments shorter than   36093 bp
  90% of sequence in fragments shorter than   44891 bp
  95% of sequence in fragments shorter than   63453 bp

# EciI.RsrII (GGCGGA+CGGWCCG) double digest, 580 thousand fragments (571273+9764 sites), 5131 bp average fragment length
   5% of sequence in fragments shorter than    1653 bp
  10% of sequence in fragments shorter than    2731 bp
  15% of sequence in fragments shorter than    3745 bp
  20% of sequence in fragments shorter than    4793 bp
  25% of sequence in fragments shorter than    5887 bp
  30% of sequence in fragments shorter than    7052 bp
  35% of sequence in fragments shorter than    8301 bp
  40% of sequence in fragments shorter than    9673 bp
  45% of sequence in fragments shorter than   11192 bp
  50% of sequence in fragments shorter than   12877 bp
  55% of sequence in fragments shorter than   14784 bp
  60% of sequence in fragments shorter than   16936 bp
  65% of sequence in fragments shorter than   19386 bp
  70% of sequence in fragments shorter than   22255 bp
  75% of sequence in fragments shorter than   25744 bp
  80% of sequence in fragments shorter than   29991 bp
  85% of sequence in fragments shorter than   35823 bp
  90% of sequence in fragments shorter than   44521 bp
  95% of sequence in fragments shorter than   62847 bp

# MfeI.SgrAI (CAATTG+CRCCGGYG) double digest, 579 thousand fragments (564303+15270 sites), 5145 bp average fragment length
   5% of sequence in fragments shorter than    1788 bp
  10% of sequence in fragments shorter than    2686 bp
  15% of sequence in fragments shorter than    3487 bp
  20% of sequence in fragments shorter than    4250 bp
  25% of sequence in fragments shorter than    4978 bp
  30% of sequence in fragments shorter than    5711 bp
  35% of sequence in fragments shorter than    6460 bp
  40% of sequence in fragments shorter than    7242 bp
  45% of sequence in fragments shorter than    8076 bp
  50% of sequence in fragments shorter than    8966 bp
  55% of sequence in fragments shorter than    9953 bp
  60% of sequence in fragments shorter than   11025 bp
  65% of sequence in fragments shorter than   12211 bp
  70% of sequence in fragments shorter than   13587 bp
  75% of sequence in fragments shorter than   15231 bp
  80% of sequence in fragments shorter than   17249 bp
  85% of sequence in fragments shorter than   20007 bp
  90% of sequence in fragments shorter than   24136 bp
  95% of sequence in fragments shorter than   32449 bp

# MfeI.NruI (CAATTG+TCGCGA) double digest, 578 thousand fragments (564303+14872 sites), 5149 bp average fragment length
   5% of sequence in fragments shorter than    1786 bp
  10% of sequence in fragments shorter than    2685 bp
  15% of sequence in fragments shorter than    3486 bp
  20% of sequence in fragments shorter than    4247 bp
  25% of sequence in fragments shorter than    4974 bp
  30% of sequence in fragments shorter than    5706 bp
  35% of sequence in fragments shorter than    6458 bp
  40% of sequence in fragments shorter than    7246 bp
  45% of sequence in fragments shorter than    8080 bp
  50% of sequence in fragments shorter than    8966 bp
  55% of sequence in fragments shorter than    9958 bp
  60% of sequence in fragments shorter than   11030 bp
  65% of sequence in fragments shorter than   12224 bp
  70% of sequence in fragments shorter than   13601 bp
  75% of sequence in fragments shorter than   15250 bp
  80% of sequence in fragments shorter than   17318 bp
  85% of sequence in fragments shorter than   20127 bp
  90% of sequence in fragments shorter than   24414 bp
  95% of sequence in fragments shorter than   32939 bp

# EcoRV.SnaBI (GATATC+TACGTA) double digest, 578 thousand fragments (446473+132299 sites), 5152 bp average fragment length
   5% of sequence in fragments shorter than    1761 bp
  10% of sequence in fragments shorter than    2720 bp
  15% of sequence in fragments shorter than    3549 bp
  20% of sequence in fragments shorter than    4335 bp
  25% of sequence in fragments shorter than    5102 bp
  30% of sequence in fragments shorter than    5876 bp
  35% of sequence in fragments shorter than    6667 bp
  40% of sequence in fragments shorter than    7491 bp
  45% of sequence in fragments shorter than    8365 bp
  50% of sequence in fragments shorter than    9295 bp
  55% of sequence in fragments shorter than   10313 bp
  60% of sequence in fragments shorter than   11423 bp
  65% of sequence in fragments shorter than   12688 bp
  70% of sequence in fragments shorter than   14148 bp
  75% of sequence in fragments shorter than   15873 bp
  80% of sequence in fragments shorter than   18009 bp
  85% of sequence in fragments shorter than   20934 bp
  90% of sequence in fragments shorter than   25395 bp
  95% of sequence in fragments shorter than   34454 bp

# MfeI.PvuI (CAATTG+CGATCG) double digest, 578 thousand fragments (564303+14186 sites), 5155 bp average fragment length
   5% of sequence in fragments shorter than    1787 bp
  10% of sequence in fragments shorter than    2679 bp
  15% of sequence in fragments shorter than    3481 bp
  20% of sequence in fragments shorter than    4244 bp
  25% of sequence in fragments shorter than    4975 bp
  30% of sequence in fragments shorter than    5710 bp
  35% of sequence in fragments shorter than    6464 bp
  40% of sequence in fragments shorter than    7252 bp
  45% of sequence in fragments shorter than    8088 bp
  50% of sequence in fragments shorter than    8984 bp
  55% of sequence in fragments shorter than    9976 bp
  60% of sequence in fragments shorter than   11061 bp
  65% of sequence in fragments shorter than   12267 bp
  70% of sequence in fragments shorter than   13657 bp
  75% of sequence in fragments shorter than   15328 bp
  80% of sequence in fragments shorter than   17396 bp
  85% of sequence in fragments shorter than   20214 bp
  90% of sequence in fragments shorter than   24544 bp
  95% of sequence in fragments shorter than   33201 bp

# FseI.MfeI (GGCCGGCC+CAATTG) double digest, 577 thousand fragments (13662+564303 sites), 5159 bp average fragment length
   5% of sequence in fragments shorter than    1795 bp
  10% of sequence in fragments shorter than    2697 bp
  15% of sequence in fragments shorter than    3499 bp
  20% of sequence in fragments shorter than    4262 bp
  25% of sequence in fragments shorter than    4991 bp
  30% of sequence in fragments shorter than    5725 bp
  35% of sequence in fragments shorter than    6478 bp
  40% of sequence in fragments shorter than    7262 bp
  45% of sequence in fragments shorter than    8095 bp
  50% of sequence in fragments shorter than    8980 bp
  55% of sequence in fragments shorter than    9964 bp
  60% of sequence in fragments shorter than   11031 bp
  65% of sequence in fragments shorter than   12214 bp
  70% of sequence in fragments shorter than   13586 bp
  75% of sequence in fragments shorter than   15231 bp
  80% of sequence in fragments shorter than   17237 bp
  85% of sequence in fragments shorter than   19938 bp
  90% of sequence in fragments shorter than   24067 bp
  95% of sequence in fragments shorter than   32260 bp

# AscI.HgaI (GGCGCGCC+GACGC) double digest, 576 thousand fragments (4751+571889 sites), 5171 bp average fragment length
   5% of sequence in fragments shorter than    1736 bp
  10% of sequence in fragments shorter than    2906 bp
  15% of sequence in fragments shorter than    3992 bp
  20% of sequence in fragments shorter than    5082 bp
  25% of sequence in fragments shorter than    6171 bp
  30% of sequence in fragments shorter than    7285 bp
  35% of sequence in fragments shorter than    8464 bp
  40% of sequence in fragments shorter than    9712 bp
  45% of sequence in fragments shorter than   11057 bp
  50% of sequence in fragments shorter than   12525 bp
  55% of sequence in fragments shorter than   14153 bp
  60% of sequence in fragments shorter than   15907 bp
  65% of sequence in fragments shorter than   17861 bp
  70% of sequence in fragments shorter than   20176 bp
  75% of sequence in fragments shorter than   22829 bp
  80% of sequence in fragments shorter than   26187 bp
  85% of sequence in fragments shorter than   30581 bp
  90% of sequence in fragments shorter than   36977 bp
  95% of sequence in fragments shorter than   49619 bp

# AscI.EciI (GGCGCGCC+GGCGGA) double digest, 575 thousand fragments (4751+571273 sites), 5176 bp average fragment length
   5% of sequence in fragments shorter than    1665 bp
  10% of sequence in fragments shorter than    2753 bp
  15% of sequence in fragments shorter than    3776 bp
  20% of sequence in fragments shorter than    4832 bp
  25% of sequence in fragments shorter than    5936 bp
  30% of sequence in fragments shorter than    7109 bp
  35% of sequence in fragments shorter than    8366 bp
  40% of sequence in fragments shorter than    9755 bp
  45% of sequence in fragments shorter than   11282 bp
  50% of sequence in fragments shorter than   12974 bp
  55% of sequence in fragments shorter than   14901 bp
  60% of sequence in fragments shorter than   17076 bp
  65% of sequence in fragments shorter than   19533 bp
  70% of sequence in fragments shorter than   22432 bp
  75% of sequence in fragments shorter than   25924 bp
  80% of sequence in fragments shorter than   30247 bp
  85% of sequence in fragments shorter than   36142 bp
  90% of sequence in fragments shorter than   44937 bp
  95% of sequence in fragments shorter than   63453 bp

# KpnI.NheI (GGTACC+GCTAGC) double digest, 575 thousand fragments (287974+287384 sites), 5182 bp average fragment length
   5% of sequence in fragments shorter than    1718 bp
  10% of sequence in fragments shorter than    2759 bp
  15% of sequence in fragments shorter than    3668 bp
  20% of sequence in fragments shorter than    4547 bp
  25% of sequence in fragments shorter than    5393 bp
  30% of sequence in fragments shorter than    6233 bp
  35% of sequence in fragments shorter than    7081 bp
  40% of sequence in fragments shorter than    7941 bp
  45% of sequence in fragments shorter than    8855 bp
  50% of sequence in fragments shorter than    9821 bp
  55% of sequence in fragments shorter than   10851 bp
  60% of sequence in fragments shorter than   11981 bp
  65% of sequence in fragments shorter than   13242 bp
  70% of sequence in fragments shorter than   14670 bp
  75% of sequence in fragments shorter than   16322 bp
  80% of sequence in fragments shorter than   18341 bp
  85% of sequence in fragments shorter than   20981 bp
  90% of sequence in fragments shorter than   24830 bp
  95% of sequence in fragments shorter than   32472 bp

# SalI.ScaI (GTCGAC+AGTACT) double digest, 574 thousand fragments (31396+543793 sites), 5184 bp average fragment length
   5% of sequence in fragments shorter than    1790 bp
  10% of sequence in fragments shorter than    2707 bp
  15% of sequence in fragments shorter than    3511 bp
  20% of sequence in fragments shorter than    4275 bp
  25% of sequence in fragments shorter than    5025 bp
  30% of sequence in fragments shorter than    5785 bp
  35% of sequence in fragments shorter than    6559 bp
  40% of sequence in fragments shorter than    7365 bp
  45% of sequence in fragments shorter than    8198 bp
  50% of sequence in fragments shorter than    9088 bp
  55% of sequence in fragments shorter than   10037 bp
  60% of sequence in fragments shorter than   11092 bp
  65% of sequence in fragments shorter than   12304 bp
  70% of sequence in fragments shorter than   13694 bp
  75% of sequence in fragments shorter than   15292 bp
  80% of sequence in fragments shorter than   17310 bp
  85% of sequence in fragments shorter than   20012 bp
  90% of sequence in fragments shorter than   24002 bp
  95% of sequence in fragments shorter than   32568 bp

# MfeI.NotI (CAATTG+GCGGCCGC) double digest, 573 thousand fragments (564303+9795 sites), 5194 bp average fragment length
   5% of sequence in fragments shorter than    1803 bp
  10% of sequence in fragments shorter than    2712 bp
  15% of sequence in fragments shorter than    3523 bp
  20% of sequence in fragments shorter than    4291 bp
  25% of sequence in fragments shorter than    5025 bp
  30% of sequence in fragments shorter than    5769 bp
  35% of sequence in fragments shorter than    6526 bp
  40% of sequence in fragments shorter than    7323 bp
  45% of sequence in fragments shorter than    8165 bp
  50% of sequence in fragments shorter than    9065 bp
  55% of sequence in fragments shorter than   10075 bp
  60% of sequence in fragments shorter than   11158 bp
  65% of sequence in fragments shorter than   12372 bp
  70% of sequence in fragments shorter than   13767 bp
  75% of sequence in fragments shorter than   15450 bp
  80% of sequence in fragments shorter than   17528 bp
  85% of sequence in fragments shorter than   20370 bp
  90% of sequence in fragments shorter than   24626 bp
  95% of sequence in fragments shorter than   33282 bp

# MfeI.RsrII (CAATTG+CGGWCCG) double digest, 573 thousand fragments (564303+9764 sites), 5194 bp average fragment length
   5% of sequence in fragments shorter than    1804 bp
  10% of sequence in fragments shorter than    2709 bp
  15% of sequence in fragments shorter than    3518 bp
  20% of sequence in fragments shorter than    4283 bp
  25% of sequence in fragments shorter than    5016 bp
  30% of sequence in fragments shorter than    5757 bp
  35% of sequence in fragments shorter than    6517 bp
  40% of sequence in fragments shorter than    7311 bp
  45% of sequence in fragments shorter than    8153 bp
  50% of sequence in fragments shorter than    9051 bp
  55% of sequence in fragments shorter than   10055 bp
  60% of sequence in fragments shorter than   11139 bp
  65% of sequence in fragments shorter than   12350 bp
  70% of sequence in fragments shorter than   13749 bp
  75% of sequence in fragments shorter than   15436 bp
  80% of sequence in fragments shorter than   17508 bp
  85% of sequence in fragments shorter than   20372 bp
  90% of sequence in fragments shorter than   24702 bp
  95% of sequence in fragments shorter than   33341 bp

# AclI.SpeI (AACGTT+ACTAGT) double digest, 572 thousand fragments (172706+400286 sites), 5204 bp average fragment length
   5% of sequence in fragments shorter than    1787 bp
  10% of sequence in fragments shorter than    2706 bp
  15% of sequence in fragments shorter than    3508 bp
  20% of sequence in fragments shorter than    4276 bp
  25% of sequence in fragments shorter than    5033 bp
  30% of sequence in fragments shorter than    5786 bp
  35% of sequence in fragments shorter than    6562 bp
  40% of sequence in fragments shorter than    7351 bp
  45% of sequence in fragments shorter than    8202 bp
  50% of sequence in fragments shorter than    9090 bp
  55% of sequence in fragments shorter than   10057 bp
  60% of sequence in fragments shorter than   11130 bp
  65% of sequence in fragments shorter than   12331 bp
  70% of sequence in fragments shorter than   13704 bp
  75% of sequence in fragments shorter than   15345 bp
  80% of sequence in fragments shorter than   17336 bp
  85% of sequence in fragments shorter than   20007 bp
  90% of sequence in fragments shorter than   24018 bp
  95% of sequence in fragments shorter than   31952 bp

# MluI.SphI (ACGCGT+GCATGC) double digest, 571 thousand fragments (21415+550952 sites), 5209 bp average fragment length
   5% of sequence in fragments shorter than    1817 bp
  10% of sequence in fragments shorter than    2748 bp
  15% of sequence in fragments shorter than    3568 bp
  20% of sequence in fragments shorter than    4344 bp
  25% of sequence in fragments shorter than    5115 bp
  30% of sequence in fragments shorter than    5877 bp
  35% of sequence in fragments shorter than    6660 bp
  40% of sequence in fragments shorter than    7463 bp
  45% of sequence in fragments shorter than    8301 bp
  50% of sequence in fragments shorter than    9203 bp
  55% of sequence in fragments shorter than   10191 bp
  60% of sequence in fragments shorter than   11288 bp
  65% of sequence in fragments shorter than   12506 bp
  70% of sequence in fragments shorter than   13883 bp
  75% of sequence in fragments shorter than   15508 bp
  80% of sequence in fragments shorter than   17472 bp
  85% of sequence in fragments shorter than   20075 bp
  90% of sequence in fragments shorter than   23887 bp
  95% of sequence in fragments shorter than   31368 bp

# EcoRV.NaeI (GATATC+GCCGGC) double digest, 571 thousand fragments (446473+125692 sites), 5212 bp average fragment length
   5% of sequence in fragments shorter than    1837 bp
  10% of sequence in fragments shorter than    2835 bp
  15% of sequence in fragments shorter than    3706 bp
  20% of sequence in fragments shorter than    4511 bp
  25% of sequence in fragments shorter than    5288 bp
  30% of sequence in fragments shorter than    6064 bp
  35% of sequence in fragments shorter than    6857 bp
  40% of sequence in fragments shorter than    7671 bp
  45% of sequence in fragments shorter than    8521 bp
  50% of sequence in fragments shorter than    9423 bp
  55% of sequence in fragments shorter than   10388 bp
  60% of sequence in fragments shorter than   11458 bp
  65% of sequence in fragments shorter than   12615 bp
  70% of sequence in fragments shorter than   13928 bp
  75% of sequence in fragments shorter than   15475 bp
  80% of sequence in fragments shorter than   17320 bp
  85% of sequence in fragments shorter than   19716 bp
  90% of sequence in fragments shorter than   23125 bp
  95% of sequence in fragments shorter than   29446 bp

# BsrBI.BstEII (CCGCTC+GGTNACC) double digest, 570 thousand fragments (235523+335865 sites), 5219 bp average fragment length
   5% of sequence in fragments shorter than    1748 bp
  10% of sequence in fragments shorter than    2815 bp
  15% of sequence in fragments shorter than    3768 bp
  20% of sequence in fragments shorter than    4687 bp
  25% of sequence in fragments shorter than    5592 bp
  30% of sequence in fragments shorter than    6511 bp
  35% of sequence in fragments shorter than    7462 bp
  40% of sequence in fragments shorter than    8445 bp
  45% of sequence in fragments shorter than    9497 bp
  50% of sequence in fragments shorter than   10622 bp
  55% of sequence in fragments shorter than   11831 bp
  60% of sequence in fragments shorter than   13200 bp
  65% of sequence in fragments shorter than   14722 bp
  70% of sequence in fragments shorter than   16447 bp
  75% of sequence in fragments shorter than   18490 bp
  80% of sequence in fragments shorter than   20976 bp
  85% of sequence in fragments shorter than   24345 bp
  90% of sequence in fragments shorter than   29215 bp
  95% of sequence in fragments shorter than   38414 bp

# AscI.MfeI (GGCGCGCC+CAATTG) double digest, 568 thousand fragments (4751+564303 sites), 5240 bp average fragment length
   5% of sequence in fragments shorter than    1815 bp
  10% of sequence in fragments shorter than    2728 bp
  15% of sequence in fragments shorter than    3545 bp
  20% of sequence in fragments shorter than    4318 bp
  25% of sequence in fragments shorter than    5060 bp
  30% of sequence in fragments shorter than    5809 bp
  35% of sequence in fragments shorter than    6578 bp
  40% of sequence in fragments shorter than    7384 bp
  45% of sequence in fragments shorter than    8237 bp
  50% of sequence in fragments shorter than    9150 bp
  55% of sequence in fragments shorter than   10174 bp
  60% of sequence in fragments shorter than   11271 bp
  65% of sequence in fragments shorter than   12504 bp
  70% of sequence in fragments shorter than   13942 bp
  75% of sequence in fragments shorter than   15668 bp
  80% of sequence in fragments shorter than   17815 bp
  85% of sequence in fragments shorter than   20803 bp
  90% of sequence in fragments shorter than   25329 bp
  95% of sequence in fragments shorter than   34595 bp

# EcoRV.XhoI (GATATC+CTCGAG) double digest, 567 thousand fragments (446473+121308 sites), 5252 bp average fragment length
   5% of sequence in fragments shorter than    1794 bp
  10% of sequence in fragments shorter than    2770 bp
  15% of sequence in fragments shorter than    3626 bp
  20% of sequence in fragments shorter than    4420 bp
  25% of sequence in fragments shorter than    5186 bp
  30% of sequence in fragments shorter than    5948 bp
  35% of sequence in fragments shorter than    6727 bp
  40% of sequence in fragments shorter than    7534 bp
  45% of sequence in fragments shorter than    8372 bp
  50% of sequence in fragments shorter than    9252 bp
  55% of sequence in fragments shorter than   10201 bp
  60% of sequence in fragments shorter than   11239 bp
  65% of sequence in fragments shorter than   12382 bp
  70% of sequence in fragments shorter than   13659 bp
  75% of sequence in fragments shorter than   15162 bp
  80% of sequence in fragments shorter than   16977 bp
  85% of sequence in fragments shorter than   19343 bp
  90% of sequence in fragments shorter than   22697 bp
  95% of sequence in fragments shorter than   28958 bp

# AatII.ApaLI (GACGTC+GTGCAC) double digest, 566 thousand fragments (71089+496255 sites), 5256 bp average fragment length
   5% of sequence in fragments shorter than    1845 bp
  10% of sequence in fragments shorter than    2804 bp
  15% of sequence in fragments shorter than    3646 bp
  20% of sequence in fragments shorter than    4446 bp
  25% of sequence in fragments shorter than    5224 bp
  30% of sequence in fragments shorter than    5992 bp
  35% of sequence in fragments shorter than    6782 bp
  40% of sequence in fragments shorter than    7616 bp
  45% of sequence in fragments shorter than    8487 bp
  50% of sequence in fragments shorter than    9415 bp
  55% of sequence in fragments shorter than   10427 bp
  60% of sequence in fragments shorter than   11540 bp
  65% of sequence in fragments shorter than   12783 bp
  70% of sequence in fragments shorter than   14199 bp
  75% of sequence in fragments shorter than   15852 bp
  80% of sequence in fragments shorter than   17895 bp
  85% of sequence in fragments shorter than   20575 bp
  90% of sequence in fragments shorter than   24444 bp
  95% of sequence in fragments shorter than   31851 bp

# BstEII.KasI (GGTNACC+GGCGCC) double digest, 565 thousand fragments (335865+230381 sites), 5265 bp average fragment length
   5% of sequence in fragments shorter than    1763 bp
  10% of sequence in fragments shorter than    2801 bp
  15% of sequence in fragments shorter than    3717 bp
  20% of sequence in fragments shorter than    4596 bp
  25% of sequence in fragments shorter than    5460 bp
  30% of sequence in fragments shorter than    6328 bp
  35% of sequence in fragments shorter than    7224 bp
  40% of sequence in fragments shorter than    8172 bp
  45% of sequence in fragments shorter than    9170 bp
  50% of sequence in fragments shorter than   10244 bp
  55% of sequence in fragments shorter than   11410 bp
  60% of sequence in fragments shorter than   12699 bp
  65% of sequence in fragments shorter than   14170 bp
  70% of sequence in fragments shorter than   15861 bp
  75% of sequence in fragments shorter than   17848 bp
  80% of sequence in fragments shorter than   20294 bp
  85% of sequence in fragments shorter than   23579 bp
  90% of sequence in fragments shorter than   28609 bp
  95% of sequence in fragments shorter than   38957 bp

# SgrAI.SphI (CRCCGGYG+GCATGC) double digest, 565 thousand fragments (15270+550952 sites), 5266 bp average fragment length
   5% of sequence in fragments shorter than    1838 bp
  10% of sequence in fragments shorter than    2777 bp
  15% of sequence in fragments shorter than    3605 bp
  20% of sequence in fragments shorter than    4392 bp
  25% of sequence in fragments shorter than    5166 bp
  30% of sequence in fragments shorter than    5937 bp
  35% of sequence in fragments shorter than    6725 bp
  40% of sequence in fragments shorter than    7535 bp
  45% of sequence in fragments shorter than    8385 bp
  50% of sequence in fragments shorter than    9299 bp
  55% of sequence in fragments shorter than   10291 bp
  60% of sequence in fragments shorter than   11396 bp
  65% of sequence in fragments shorter than   12623 bp
  70% of sequence in fragments shorter than   14012 bp
  75% of sequence in fragments shorter than   15647 bp
  80% of sequence in fragments shorter than   17647 bp
  85% of sequence in fragments shorter than   20280 bp
  90% of sequence in fragments shorter than   24163 bp
  95% of sequence in fragments shorter than   31797 bp

# NruI.SphI (TCGCGA+GCATGC) double digest, 565 thousand fragments (14872+550952 sites), 5270 bp average fragment length
   5% of sequence in fragments shorter than    1839 bp
  10% of sequence in fragments shorter than    2778 bp
  15% of sequence in fragments shorter than    3608 bp
  20% of sequence in fragments shorter than    4392 bp
  25% of sequence in fragments shorter than    5168 bp
  30% of sequence in fragments shorter than    5937 bp
  35% of sequence in fragments shorter than    6723 bp
  40% of sequence in fragments shorter than    7532 bp
  45% of sequence in fragments shorter than    8377 bp
  50% of sequence in fragments shorter than    9285 bp
  55% of sequence in fragments shorter than   10272 bp
  60% of sequence in fragments shorter than   11372 bp
  65% of sequence in fragments shorter than   12589 bp
  70% of sequence in fragments shorter than   13977 bp
  75% of sequence in fragments shorter than   15613 bp
  80% of sequence in fragments shorter than   17591 bp
  85% of sequence in fragments shorter than   20200 bp
  90% of sequence in fragments shorter than   24050 bp
  95% of sequence in fragments shorter than   31643 bp

# MluI.ScaI (ACGCGT+AGTACT) double digest, 564 thousand fragments (21415+543793 sites), 5275 bp average fragment length
   5% of sequence in fragments shorter than    1826 bp
  10% of sequence in fragments shorter than    2759 bp
  15% of sequence in fragments shorter than    3577 bp
  20% of sequence in fragments shorter than    4356 bp
  25% of sequence in fragments shorter than    5117 bp
  30% of sequence in fragments shorter than    5891 bp
  35% of sequence in fragments shorter than    6681 bp
  40% of sequence in fragments shorter than    7504 bp
  45% of sequence in fragments shorter than    8360 bp
  50% of sequence in fragments shorter than    9270 bp
  55% of sequence in fragments shorter than   10238 bp
  60% of sequence in fragments shorter than   11320 bp
  65% of sequence in fragments shorter than   12542 bp
  70% of sequence in fragments shorter than   13945 bp
  75% of sequence in fragments shorter than   15553 bp
  80% of sequence in fragments shorter than   17555 bp
  85% of sequence in fragments shorter than   20250 bp
  90% of sequence in fragments shorter than   24240 bp
  95% of sequence in fragments shorter than   32668 bp

# PvuI.SphI (CGATCG+GCATGC) double digest, 564 thousand fragments (14186+550952 sites), 5276 bp average fragment length
   5% of sequence in fragments shorter than    1843 bp
  10% of sequence in fragments shorter than    2776 bp
  15% of sequence in fragments shorter than    3610 bp
  20% of sequence in fragments shorter than    4397 bp
  25% of sequence in fragments shorter than    5173 bp
  30% of sequence in fragments shorter than    5945 bp
  35% of sequence in fragments shorter than    6733 bp
  40% of sequence in fragments shorter than    7539 bp
  45% of sequence in fragments shorter than    8386 bp
  50% of sequence in fragments shorter than    9297 bp
  55% of sequence in fragments shorter than   10284 bp
  60% of sequence in fragments shorter than   11382 bp
  65% of sequence in fragments shorter than   12600 bp
  70% of sequence in fragments shorter than   13984 bp
  75% of sequence in fragments shorter than   15610 bp
  80% of sequence in fragments shorter than   17587 bp
  85% of sequence in fragments shorter than   20191 bp
  90% of sequence in fragments shorter than   24006 bp
  95% of sequence in fragments shorter than   31368 bp

# FseI.SphI (GGCCGGCC+GCATGC) double digest, 564 thousand fragments (13662+550952 sites), 5281 bp average fragment length
   5% of sequence in fragments shorter than    1841 bp
  10% of sequence in fragments shorter than    2781 bp
  15% of sequence in fragments shorter than    3614 bp
  20% of sequence in fragments shorter than    4402 bp
  25% of sequence in fragments shorter than    5183 bp
  30% of sequence in fragments shorter than    5956 bp
  35% of sequence in fragments shorter than    6748 bp
  40% of sequence in fragments shorter than    7561 bp
  45% of sequence in fragments shorter than    8408 bp
  50% of sequence in fragments shorter than    9321 bp
  55% of sequence in fragments shorter than   10321 bp
  60% of sequence in fragments shorter than   11437 bp
  65% of sequence in fragments shorter than   12658 bp
  70% of sequence in fragments shorter than   14066 bp
  75% of sequence in fragments shorter than   15723 bp
  80% of sequence in fragments shorter than   17716 bp
  85% of sequence in fragments shorter than   20338 bp
  90% of sequence in fragments shorter than   24243 bp
  95% of sequence in fragments shorter than   31952 bp

# ApaLI.SacII (GTGCAC+CCGCGG) double digest, 562 thousand fragments (496255+66901 sites), 5295 bp average fragment length
   5% of sequence in fragments shorter than    1891 bp
  10% of sequence in fragments shorter than    2916 bp
  15% of sequence in fragments shorter than    3818 bp
  20% of sequence in fragments shorter than    4675 bp
  25% of sequence in fragments shorter than    5512 bp
  30% of sequence in fragments shorter than    6322 bp
  35% of sequence in fragments shorter than    7182 bp
  40% of sequence in fragments shorter than    8080 bp
  45% of sequence in fragments shorter than    9021 bp
  50% of sequence in fragments shorter than    9993 bp
  55% of sequence in fragments shorter than   11089 bp
  60% of sequence in fragments shorter than   12297 bp
  65% of sequence in fragments shorter than   13654 bp
  70% of sequence in fragments shorter than   15191 bp
  75% of sequence in fragments shorter than   17006 bp
  80% of sequence in fragments shorter than   19241 bp
  85% of sequence in fragments shorter than   22189 bp
  90% of sequence in fragments shorter than   26418 bp
  95% of sequence in fragments shorter than   34732 bp

# BstEII.SwaI (GGTNACC+ATTTAAAT) double digest, 562 thousand fragments (335865+226602 sites), 5300 bp average fragment length
   5% of sequence in fragments shorter than    1882 bp
  10% of sequence in fragments shorter than    2842 bp
  15% of sequence in fragments shorter than    3661 bp
  20% of sequence in fragments shorter than    4435 bp
  25% of sequence in fragments shorter than    5171 bp
  30% of sequence in fragments shorter than    5914 bp
  35% of sequence in fragments shorter than    6669 bp
  40% of sequence in fragments shorter than    7444 bp
  45% of sequence in fragments shorter than    8247 bp
  50% of sequence in fragments shorter than    9105 bp
  55% of sequence in fragments shorter than   10028 bp
  60% of sequence in fragments shorter than   11028 bp
  65% of sequence in fragments shorter than   12152 bp
  70% of sequence in fragments shorter than   13440 bp
  75% of sequence in fragments shorter than   14932 bp
  80% of sequence in fragments shorter than   16763 bp
  85% of sequence in fragments shorter than   19124 bp
  90% of sequence in fragments shorter than   22588 bp
  95% of sequence in fragments shorter than   29972 bp

# AclI.HpaI (AACGTT+GTTAAC) double digest, 561 thousand fragments (172706+389418 sites), 5305 bp average fragment length
   5% of sequence in fragments shorter than    1855 bp
  10% of sequence in fragments shorter than    2808 bp
  15% of sequence in fragments shorter than    3618 bp
  20% of sequence in fragments shorter than    4403 bp
  25% of sequence in fragments shorter than    5169 bp
  30% of sequence in fragments shorter than    5936 bp
  35% of sequence in fragments shorter than    6724 bp
  40% of sequence in fragments shorter than    7538 bp
  45% of sequence in fragments shorter than    8393 bp
  50% of sequence in fragments shorter than    9303 bp
  55% of sequence in fragments shorter than   10286 bp
  60% of sequence in fragments shorter than   11358 bp
  65% of sequence in fragments shorter than   12550 bp
  70% of sequence in fragments shorter than   13921 bp
  75% of sequence in fragments shorter than   15516 bp
  80% of sequence in fragments shorter than   17497 bp
  85% of sequence in fragments shorter than   20056 bp
  90% of sequence in fragments shorter than   23852 bp
  95% of sequence in fragments shorter than   31312 bp

# NotI.SphI (GCGGCCGC+GCATGC) double digest, 560 thousand fragments (9795+550952 sites), 5317 bp average fragment length
   5% of sequence in fragments shorter than    1858 bp
  10% of sequence in fragments shorter than    2807 bp
  15% of sequence in fragments shorter than    3644 bp
  20% of sequence in fragments shorter than    4437 bp
  25% of sequence in fragments shorter than    5219 bp
  30% of sequence in fragments shorter than    5997 bp
  35% of sequence in fragments shorter than    6793 bp
  40% of sequence in fragments shorter than    7609 bp
  45% of sequence in fragments shorter than    8463 bp
  50% of sequence in fragments shorter than    9383 bp
  55% of sequence in fragments shorter than   10382 bp
  60% of sequence in fragments shorter than   11499 bp
  65% of sequence in fragments shorter than   12726 bp
  70% of sequence in fragments shorter than   14136 bp
  75% of sequence in fragments shorter than   15793 bp
  80% of sequence in fragments shorter than   17793 bp
  85% of sequence in fragments shorter than   20449 bp
  90% of sequence in fragments shorter than   24362 bp
  95% of sequence in fragments shorter than   32019 bp

# RsrII.SphI (CGGWCCG+GCATGC) double digest, 560 thousand fragments (9764+550952 sites), 5318 bp average fragment length
   5% of sequence in fragments shorter than    1857 bp
  10% of sequence in fragments shorter than    2805 bp
  15% of sequence in fragments shorter than    3641 bp
  20% of sequence in fragments shorter than    4434 bp
  25% of sequence in fragments shorter than    5216 bp
  30% of sequence in fragments shorter than    5993 bp
  35% of sequence in fragments shorter than    6787 bp
  40% of sequence in fragments shorter than    7600 bp
  45% of sequence in fragments shorter than    8452 bp
  50% of sequence in fragments shorter than    9369 bp
  55% of sequence in fragments shorter than   10367 bp
  60% of sequence in fragments shorter than   11480 bp
  65% of sequence in fragments shorter than   12706 bp
  70% of sequence in fragments shorter than   14110 bp
  75% of sequence in fragments shorter than   15759 bp
  80% of sequence in fragments shorter than   17751 bp
  85% of sequence in fragments shorter than   20387 bp
  90% of sequence in fragments shorter than   24287 bp
  95% of sequence in fragments shorter than   31947 bp

# PacI.SpeI (TTAATTAA+ACTAGT) double digest, 559 thousand fragments (159455+400286 sites), 5327 bp average fragment length
   5% of sequence in fragments shorter than    1863 bp
  10% of sequence in fragments shorter than    2807 bp
  15% of sequence in fragments shorter than    3658 bp
  20% of sequence in fragments shorter than    4458 bp
  25% of sequence in fragments shorter than    5248 bp
  30% of sequence in fragments shorter than    6041 bp
  35% of sequence in fragments shorter than    6837 bp
  40% of sequence in fragments shorter than    7664 bp
  45% of sequence in fragments shorter than    8547 bp
  50% of sequence in fragments shorter than    9509 bp
  55% of sequence in fragments shorter than   10551 bp
  60% of sequence in fragments shorter than   11727 bp
  65% of sequence in fragments shorter than   13049 bp
  70% of sequence in fragments shorter than   14586 bp
  75% of sequence in fragments shorter than   16478 bp
  80% of sequence in fragments shorter than   18812 bp
  85% of sequence in fragments shorter than   22039 bp
  90% of sequence in fragments shorter than   27257 bp
  95% of sequence in fragments shorter than   39831 bp

# ScaI.SgrAI (AGTACT+CRCCGGYG) double digest, 558 thousand fragments (543793+15270 sites), 5333 bp average fragment length
   5% of sequence in fragments shorter than    1842 bp
  10% of sequence in fragments shorter than    2784 bp
  15% of sequence in fragments shorter than    3616 bp
  20% of sequence in fragments shorter than    4404 bp
  25% of sequence in fragments shorter than    5175 bp
  30% of sequence in fragments shorter than    5959 bp
  35% of sequence in fragments shorter than    6760 bp
  40% of sequence in fragments shorter than    7588 bp
  45% of sequence in fragments shorter than    8452 bp
  50% of sequence in fragments shorter than    9372 bp
  55% of sequence in fragments shorter than   10361 bp
  60% of sequence in fragments shorter than   11472 bp
  65% of sequence in fragments shorter than   12711 bp
  70% of sequence in fragments shorter than   14139 bp
  75% of sequence in fragments shorter than   15797 bp
  80% of sequence in fragments shorter than   17824 bp
  85% of sequence in fragments shorter than   20614 bp
  90% of sequence in fragments shorter than   24746 bp
  95% of sequence in fragments shorter than   33481 bp

# NruI.ScaI (TCGCGA+AGTACT) double digest, 558 thousand fragments (14872+543793 sites), 5337 bp average fragment length
   5% of sequence in fragments shorter than    1841 bp
  10% of sequence in fragments shorter than    2783 bp
  15% of sequence in fragments shorter than    3616 bp
  20% of sequence in fragments shorter than    4403 bp
  25% of sequence in fragments shorter than    5173 bp
  30% of sequence in fragments shorter than    5955 bp
  35% of sequence in fragments shorter than    6756 bp
  40% of sequence in fragments shorter than    7589 bp
  45% of sequence in fragments shorter than    8454 bp
  50% of sequence in fragments shorter than    9381 bp
  55% of sequence in fragments shorter than   10369 bp
  60% of sequence in fragments shorter than   11489 bp
  65% of sequence in fragments shorter than   12737 bp
  70% of sequence in fragments shorter than   14182 bp
  75% of sequence in fragments shorter than   15851 bp
  80% of sequence in fragments shorter than   17937 bp
  85% of sequence in fragments shorter than   20777 bp
  90% of sequence in fragments shorter than   25024 bp
  95% of sequence in fragments shorter than   34117 bp

# Hpy99I.SpeI (CGWCG+ACTAGT) double digest, 557 thousand fragments (157917+400286 sites), 5342 bp average fragment length
   5% of sequence in fragments shorter than    1913 bp
  10% of sequence in fragments shorter than    2905 bp
  15% of sequence in fragments shorter than    3791 bp
  20% of sequence in fragments shorter than    4608 bp
  25% of sequence in fragments shorter than    5391 bp
  30% of sequence in fragments shorter than    6146 bp
  35% of sequence in fragments shorter than    6955 bp
  40% of sequence in fragments shorter than    7783 bp
  45% of sequence in fragments shorter than    8660 bp
  50% of sequence in fragments shorter than    9589 bp
  55% of sequence in fragments shorter than   10585 bp
  60% of sequence in fragments shorter than   11656 bp
  65% of sequence in fragments shorter than   12841 bp
  70% of sequence in fragments shorter than   14197 bp
  75% of sequence in fragments shorter than   15795 bp
  80% of sequence in fragments shorter than   17677 bp
  85% of sequence in fragments shorter than   20195 bp
  90% of sequence in fragments shorter than   23757 bp
  95% of sequence in fragments shorter than   30595 bp

# PvuI.ScaI (CGATCG+AGTACT) double digest, 557 thousand fragments (14186+543793 sites), 5344 bp average fragment length
   5% of sequence in fragments shorter than    1843 bp
  10% of sequence in fragments shorter than    2781 bp
  15% of sequence in fragments shorter than    3616 bp
  20% of sequence in fragments shorter than    4405 bp
  25% of sequence in fragments shorter than    5177 bp
  30% of sequence in fragments shorter than    5963 bp
  35% of sequence in fragments shorter than    6765 bp
  40% of sequence in fragments shorter than    7596 bp
  45% of sequence in fragments shorter than    8467 bp
  50% of sequence in fragments shorter than    9393 bp
  55% of sequence in fragments shorter than   10379 bp
  60% of sequence in fragments shorter than   11501 bp
  65% of sequence in fragments shorter than   12744 bp
  70% of sequence in fragments shorter than   14183 bp
  75% of sequence in fragments shorter than   15862 bp
  80% of sequence in fragments shorter than   17943 bp
  85% of sequence in fragments shorter than   20771 bp
  90% of sequence in fragments shorter than   24975 bp
  95% of sequence in fragments shorter than   34062 bp

# FseI.ScaI (GGCCGGCC+AGTACT) double digest, 557 thousand fragments (13662+543793 sites), 5349 bp average fragment length
   5% of sequence in fragments shorter than    1849 bp
  10% of sequence in fragments shorter than    2793 bp
  15% of sequence in fragments shorter than    3626 bp
  20% of sequence in fragments shorter than    4415 bp
  25% of sequence in fragments shorter than    5187 bp
  30% of sequence in fragments shorter than    5971 bp
  35% of sequence in fragments shorter than    6776 bp
  40% of sequence in fragments shorter than    7607 bp
  45% of sequence in fragments shorter than    8472 bp
  50% of sequence in fragments shorter than    9392 bp
  55% of sequence in fragments shorter than   10378 bp
  60% of sequence in fragments shorter than   11489 bp
  65% of sequence in fragments shorter than   12725 bp
  70% of sequence in fragments shorter than   14141 bp
  75% of sequence in fragments shorter than   15789 bp
  80% of sequence in fragments shorter than   17814 bp
  85% of sequence in fragments shorter than   20541 bp
  90% of sequence in fragments shorter than   24597 bp
  95% of sequence in fragments shorter than   33277 bp

# AscI.SphI (GGCGCGCC+GCATGC) double digest, 555 thousand fragments (4751+550952 sites), 5366 bp average fragment length
   5% of sequence in fragments shorter than    1874 bp
  10% of sequence in fragments shorter than    2831 bp
  15% of sequence in fragments shorter than    3675 bp
  20% of sequence in fragments shorter than    4475 bp
  25% of sequence in fragments shorter than    5263 bp
  30% of sequence in fragments shorter than    6045 bp
  35% of sequence in fragments shorter than    6848 bp
  40% of sequence in fragments shorter than    7667 bp
  45% of sequence in fragments shorter than    8529 bp
  50% of sequence in fragments shorter than    9451 bp
  55% of sequence in fragments shorter than   10457 bp
  60% of sequence in fragments shorter than   11578 bp
  65% of sequence in fragments shorter than   12808 bp
  70% of sequence in fragments shorter than   14225 bp
  75% of sequence in fragments shorter than   15888 bp
  80% of sequence in fragments shorter than   17891 bp
  85% of sequence in fragments shorter than   20554 bp
  90% of sequence in fragments shorter than   24452 bp
  95% of sequence in fragments shorter than   32114 bp

# ApaLI.PspXI (GTGCAC+VCTCGAGB) double digest, 555 thousand fragments (496255+59193 sites), 5368 bp average fragment length
   5% of sequence in fragments shorter than    1889 bp
  10% of sequence in fragments shorter than    2872 bp
  15% of sequence in fragments shorter than    3731 bp
  20% of sequence in fragments shorter than    4555 bp
  25% of sequence in fragments shorter than    5350 bp
  30% of sequence in fragments shorter than    6109 bp
  35% of sequence in fragments shorter than    6927 bp
  40% of sequence in fragments shorter than    7779 bp
  45% of sequence in fragments shorter than    8669 bp
  50% of sequence in fragments shorter than    9610 bp
  55% of sequence in fragments shorter than   10646 bp
  60% of sequence in fragments shorter than   11781 bp
  65% of sequence in fragments shorter than   13072 bp
  70% of sequence in fragments shorter than   14534 bp
  75% of sequence in fragments shorter than   16244 bp
  80% of sequence in fragments shorter than   18335 bp
  85% of sequence in fragments shorter than   21090 bp
  90% of sequence in fragments shorter than   25094 bp
  95% of sequence in fragments shorter than   33067 bp

# NotI.ScaI (GCGGCCGC+AGTACT) double digest, 553 thousand fragments (9795+543793 sites), 5386 bp average fragment length
   5% of sequence in fragments shorter than    1858 bp
  10% of sequence in fragments shorter than    2812 bp
  15% of sequence in fragments shorter than    3652 bp
  20% of sequence in fragments shorter than    4449 bp
  25% of sequence in fragments shorter than    5230 bp
  30% of sequence in fragments shorter than    6022 bp
  35% of sequence in fragments shorter than    6835 bp
  40% of sequence in fragments shorter than    7680 bp
  45% of sequence in fragments shorter than    8556 bp
  50% of sequence in fragments shorter than    9482 bp
  55% of sequence in fragments shorter than   10489 bp
  60% of sequence in fragments shorter than   11614 bp
  65% of sequence in fragments shorter than   12880 bp
  70% of sequence in fragments shorter than   14326 bp
  75% of sequence in fragments shorter than   16015 bp
  80% of sequence in fragments shorter than   18118 bp
  85% of sequence in fragments shorter than   20955 bp
  90% of sequence in fragments shorter than   25233 bp
  95% of sequence in fragments shorter than   34481 bp

# RsrII.ScaI (CGGWCCG+AGTACT) double digest, 553 thousand fragments (9764+543793 sites), 5386 bp average fragment length
   5% of sequence in fragments shorter than    1858 bp
  10% of sequence in fragments shorter than    2809 bp
  15% of sequence in fragments shorter than    3650 bp
  20% of sequence in fragments shorter than    4445 bp
  25% of sequence in fragments shorter than    5224 bp
  30% of sequence in fragments shorter than    6014 bp
  35% of sequence in fragments shorter than    6826 bp
  40% of sequence in fragments shorter than    7667 bp
  45% of sequence in fragments shorter than    8545 bp
  50% of sequence in fragments shorter than    9468 bp
  55% of sequence in fragments shorter than   10471 bp
  60% of sequence in fragments shorter than   11597 bp
  65% of sequence in fragments shorter than   12856 bp
  70% of sequence in fragments shorter than   14306 bp
  75% of sequence in fragments shorter than   15989 bp
  80% of sequence in fragments shorter than   18087 bp
  85% of sequence in fragments shorter than   20921 bp
  90% of sequence in fragments shorter than   25178 bp
  95% of sequence in fragments shorter than   34278 bp

# AclI.SapI (AACGTT+GCTCTTC) double digest, 549 thousand fragments (172706+377161 sites), 5423 bp average fragment length
   5% of sequence in fragments shorter than    1880 bp
  10% of sequence in fragments shorter than    2846 bp
  15% of sequence in fragments shorter than    3705 bp
  20% of sequence in fragments shorter than    4519 bp
  25% of sequence in fragments shorter than    5300 bp
  30% of sequence in fragments shorter than    6080 bp
  35% of sequence in fragments shorter than    6884 bp
  40% of sequence in fragments shorter than    7714 bp
  45% of sequence in fragments shorter than    8571 bp
  50% of sequence in fragments shorter than    9477 bp
  55% of sequence in fragments shorter than   10454 bp
  60% of sequence in fragments shorter than   11530 bp
  65% of sequence in fragments shorter than   12718 bp
  70% of sequence in fragments shorter than   14075 bp
  75% of sequence in fragments shorter than   15621 bp
  80% of sequence in fragments shorter than   17537 bp
  85% of sequence in fragments shorter than   19966 bp
  90% of sequence in fragments shorter than   23454 bp
  95% of sequence in fragments shorter than   30020 bp

# AgeI.ApaLI (ACCGGT+GTGCAC) double digest, 549 thousand fragments (53386+496255 sites), 5425 bp average fragment length
   5% of sequence in fragments shorter than    1911 bp
  10% of sequence in fragments shorter than    2906 bp
  15% of sequence in fragments shorter than    3773 bp
  20% of sequence in fragments shorter than    4537 bp
  25% of sequence in fragments shorter than    5340 bp
  30% of sequence in fragments shorter than    6129 bp
  35% of sequence in fragments shorter than    6955 bp
  40% of sequence in fragments shorter than    7818 bp
  45% of sequence in fragments shorter than    8710 bp
  50% of sequence in fragments shorter than    9657 bp
  55% of sequence in fragments shorter than   10683 bp
  60% of sequence in fragments shorter than   11815 bp
  65% of sequence in fragments shorter than   13114 bp
  70% of sequence in fragments shorter than   14562 bp
  75% of sequence in fragments shorter than   16261 bp
  80% of sequence in fragments shorter than   18347 bp
  85% of sequence in fragments shorter than   21096 bp
  90% of sequence in fragments shorter than   25029 bp
  95% of sequence in fragments shorter than   32828 bp

# AclI.SmaI (AACGTT+CCCGGG) double digest, 549 thousand fragments (172706+376940 sites), 5425 bp average fragment length
   5% of sequence in fragments shorter than    1767 bp
  10% of sequence in fragments shorter than    2828 bp
  15% of sequence in fragments shorter than    3778 bp
  20% of sequence in fragments shorter than    4735 bp
  25% of sequence in fragments shorter than    5688 bp
  30% of sequence in fragments shorter than    6691 bp
  35% of sequence in fragments shorter than    7713 bp
  40% of sequence in fragments shorter than    8800 bp
  45% of sequence in fragments shorter than    9947 bp
  50% of sequence in fragments shorter than   11206 bp
  55% of sequence in fragments shorter than   12557 bp
  60% of sequence in fragments shorter than   14076 bp
  65% of sequence in fragments shorter than   15778 bp
  70% of sequence in fragments shorter than   17763 bp
  75% of sequence in fragments shorter than   20138 bp
  80% of sequence in fragments shorter than   23004 bp
  85% of sequence in fragments shorter than   26793 bp
  90% of sequence in fragments shorter than   32433 bp
  95% of sequence in fragments shorter than   42863 bp

# HpaI.PacI (GTTAAC+TTAATTAA) double digest, 548 thousand fragments (389418+159455 sites), 5432 bp average fragment length
   5% of sequence in fragments shorter than    1922 bp
  10% of sequence in fragments shorter than    2918 bp
  15% of sequence in fragments shorter than    3774 bp
  20% of sequence in fragments shorter than    4578 bp
  25% of sequence in fragments shorter than    5373 bp
  30% of sequence in fragments shorter than    6170 bp
  35% of sequence in fragments shorter than    6990 bp
  40% of sequence in fragments shorter than    7858 bp
  45% of sequence in fragments shorter than    8768 bp
  50% of sequence in fragments shorter than    9719 bp
  55% of sequence in fragments shorter than   10772 bp
  60% of sequence in fragments shorter than   11931 bp
  65% of sequence in fragments shorter than   13239 bp
  70% of sequence in fragments shorter than   14749 bp
  75% of sequence in fragments shorter than   16543 bp
  80% of sequence in fragments shorter than   18820 bp
  85% of sequence in fragments shorter than   21878 bp
  90% of sequence in fragments shorter than   26647 bp
  95% of sequence in fragments shorter than   37532 bp

# AscI.ScaI (GGCGCGCC+AGTACT) double digest, 548 thousand fragments (4751+543793 sites), 5436 bp average fragment length
   5% of sequence in fragments shorter than    1872 bp
  10% of sequence in fragments shorter than    2832 bp
  15% of sequence in fragments shorter than    3679 bp
  20% of sequence in fragments shorter than    4482 bp
  25% of sequence in fragments shorter than    5269 bp
  30% of sequence in fragments shorter than    6070 bp
  35% of sequence in fragments shorter than    6890 bp
  40% of sequence in fragments shorter than    7743 bp
  45% of sequence in fragments shorter than    8627 bp
  50% of sequence in fragments shorter than    9571 bp
  55% of sequence in fragments shorter than   10593 bp
  60% of sequence in fragments shorter than   11734 bp
  65% of sequence in fragments shorter than   13021 bp
  70% of sequence in fragments shorter than   14494 bp
  75% of sequence in fragments shorter than   16222 bp
  80% of sequence in fragments shorter than   18388 bp
  85% of sequence in fragments shorter than   21321 bp
  90% of sequence in fragments shorter than   25745 bp
  95% of sequence in fragments shorter than   35500 bp

# HpaI.Hpy99I (GTTAAC+CGWCG) double digest, 546 thousand fragments (389418+157917 sites), 5448 bp average fragment length
   5% of sequence in fragments shorter than    1951 bp
  10% of sequence in fragments shorter than    2981 bp
  15% of sequence in fragments shorter than    3881 bp
  20% of sequence in fragments shorter than    4724 bp
  25% of sequence in fragments shorter than    5552 bp
  30% of sequence in fragments shorter than    6373 bp
  35% of sequence in fragments shorter than    7201 bp
  40% of sequence in fragments shorter than    8071 bp
  45% of sequence in fragments shorter than    8973 bp
  50% of sequence in fragments shorter than    9923 bp
  55% of sequence in fragments shorter than   10951 bp
  60% of sequence in fragments shorter than   12064 bp
  65% of sequence in fragments shorter than   13310 bp
  70% of sequence in fragments shorter than   14716 bp
  75% of sequence in fragments shorter than   16360 bp
  80% of sequence in fragments shorter than   18357 bp
  85% of sequence in fragments shorter than   20915 bp
  90% of sequence in fragments shorter than   24644 bp
  95% of sequence in fragments shorter than   32022 bp

# ApaI.ClaI (GGGCCC+ATCGAT) double digest, 546 thousand fragments (460339+86477 sites), 5452 bp average fragment length
   5% of sequence in fragments shorter than    1738 bp
  10% of sequence in fragments shorter than    2932 bp
  15% of sequence in fragments shorter than    4056 bp
  20% of sequence in fragments shorter than    5168 bp
  25% of sequence in fragments shorter than    6308 bp
  30% of sequence in fragments shorter than    7480 bp
  35% of sequence in fragments shorter than    8690 bp
  40% of sequence in fragments shorter than    9980 bp
  45% of sequence in fragments shorter than   11314 bp
  50% of sequence in fragments shorter than   12776 bp
  55% of sequence in fragments shorter than   14371 bp
  60% of sequence in fragments shorter than   16160 bp
  65% of sequence in fragments shorter than   18157 bp
  70% of sequence in fragments shorter than   20479 bp
  75% of sequence in fragments shorter than   23171 bp
  80% of sequence in fragments shorter than   26572 bp
  85% of sequence in fragments shorter than   31022 bp
  90% of sequence in fragments shorter than   37736 bp
  95% of sequence in fragments shorter than   51654 bp

# AclI.BamHI (AACGTT+GGATCC) double digest, 538 thousand fragments (172706+365999 sites), 5535 bp average fragment length
   5% of sequence in fragments shorter than    1939 bp
  10% of sequence in fragments shorter than    2935 bp
  15% of sequence in fragments shorter than    3790 bp
  20% of sequence in fragments shorter than    4622 bp
  25% of sequence in fragments shorter than    5426 bp
  30% of sequence in fragments shorter than    6242 bp
  35% of sequence in fragments shorter than    7057 bp
  40% of sequence in fragments shorter than    7898 bp
  45% of sequence in fragments shorter than    8788 bp
  50% of sequence in fragments shorter than    9732 bp
  55% of sequence in fragments shorter than   10733 bp
  60% of sequence in fragments shorter than   11833 bp
  65% of sequence in fragments shorter than   13058 bp
  70% of sequence in fragments shorter than   14431 bp
  75% of sequence in fragments shorter than   16049 bp
  80% of sequence in fragments shorter than   17989 bp
  85% of sequence in fragments shorter than   20495 bp
  90% of sequence in fragments shorter than   24096 bp
  95% of sequence in fragments shorter than   30860 bp

# ApaLI.PmeI (GTGCAC+GTTTAAAC) double digest, 537 thousand fragments (496255+42094 sites), 5539 bp average fragment length
   5% of sequence in fragments shorter than    1962 bp
  10% of sequence in fragments shorter than    2973 bp
  15% of sequence in fragments shorter than    3847 bp
  20% of sequence in fragments shorter than    4674 bp
  25% of sequence in fragments shorter than    5484 bp
  30% of sequence in fragments shorter than    6252 bp
  35% of sequence in fragments shorter than    7065 bp
  40% of sequence in fragments shorter than    7918 bp
  45% of sequence in fragments shorter than    8804 bp
  50% of sequence in fragments shorter than    9734 bp
  55% of sequence in fragments shorter than   10746 bp
  60% of sequence in fragments shorter than   11848 bp
  65% of sequence in fragments shorter than   13094 bp
  70% of sequence in fragments shorter than   14506 bp
  75% of sequence in fragments shorter than   16168 bp
  80% of sequence in fragments shorter than   18195 bp
  85% of sequence in fragments shorter than   20881 bp
  90% of sequence in fragments shorter than   24715 bp
  95% of sequence in fragments shorter than   32142 bp

# PacI.SapI (TTAATTAA+GCTCTTC) double digest, 536 thousand fragments (159455+377161 sites), 5556 bp average fragment length
   5% of sequence in fragments shorter than    1980 bp
  10% of sequence in fragments shorter than    3002 bp
  15% of sequence in fragments shorter than    3892 bp
  20% of sequence in fragments shorter than    4708 bp
  25% of sequence in fragments shorter than    5497 bp
  30% of sequence in fragments shorter than    6286 bp
  35% of sequence in fragments shorter than    7093 bp
  40% of sequence in fragments shorter than    7928 bp
  45% of sequence in fragments shorter than    8790 bp
  50% of sequence in fragments shorter than    9716 bp
  55% of sequence in fragments shorter than   10714 bp
  60% of sequence in fragments shorter than   11813 bp
  65% of sequence in fragments shorter than   13031 bp
  70% of sequence in fragments shorter than   14382 bp
  75% of sequence in fragments shorter than   16000 bp
  80% of sequence in fragments shorter than   17946 bp
  85% of sequence in fragments shorter than   20515 bp
  90% of sequence in fragments shorter than   24291 bp
  95% of sequence in fragments shorter than   32027 bp

# PacI.SmaI (TTAATTAA+CCCGGG) double digest, 536 thousand fragments (159455+376940 sites), 5558 bp average fragment length
   5% of sequence in fragments shorter than    1879 bp
  10% of sequence in fragments shorter than    2990 bp
  15% of sequence in fragments shorter than    3993 bp
  20% of sequence in fragments shorter than    4975 bp
  25% of sequence in fragments shorter than    5957 bp
  30% of sequence in fragments shorter than    6964 bp
  35% of sequence in fragments shorter than    8025 bp
  40% of sequence in fragments shorter than    9126 bp
  45% of sequence in fragments shorter than   10295 bp
  50% of sequence in fragments shorter than   11571 bp
  55% of sequence in fragments shorter than   12962 bp
  60% of sequence in fragments shorter than   14496 bp
  65% of sequence in fragments shorter than   16230 bp
  70% of sequence in fragments shorter than   18252 bp
  75% of sequence in fragments shorter than   20688 bp
  80% of sequence in fragments shorter than   23686 bp
  85% of sequence in fragments shorter than   27608 bp
  90% of sequence in fragments shorter than   33493 bp
  95% of sequence in fragments shorter than   46577 bp

# Hpy99I.SapI (CGWCG+GCTCTTC) double digest, 534 thousand fragments (157917+377161 sites), 5573 bp average fragment length
   5% of sequence in fragments shorter than    1912 bp
  10% of sequence in fragments shorter than    3033 bp
  15% of sequence in fragments shorter than    4029 bp
  20% of sequence in fragments shorter than    4954 bp
  25% of sequence in fragments shorter than    5863 bp
  30% of sequence in fragments shorter than    6789 bp
  35% of sequence in fragments shorter than    7733 bp
  40% of sequence in fragments shorter than    8707 bp
  45% of sequence in fragments shorter than    9742 bp
  50% of sequence in fragments shorter than   10843 bp
  55% of sequence in fragments shorter than   12048 bp
  60% of sequence in fragments shorter than   13360 bp
  65% of sequence in fragments shorter than   14808 bp
  70% of sequence in fragments shorter than   16481 bp
  75% of sequence in fragments shorter than   18441 bp
  80% of sequence in fragments shorter than   20898 bp
  85% of sequence in fragments shorter than   24075 bp
  90% of sequence in fragments shorter than   28663 bp
  95% of sequence in fragments shorter than   37916 bp

# Hpy99I.SmaI (CGWCG+CCCGGG) double digest, 534 thousand fragments (157917+376940 sites), 5575 bp average fragment length
   5% of sequence in fragments shorter than    1819 bp
  10% of sequence in fragments shorter than    3077 bp
  15% of sequence in fragments shorter than    4291 bp
  20% of sequence in fragments shorter than    5536 bp
  25% of sequence in fragments shorter than    6836 bp
  30% of sequence in fragments shorter than    8202 bp
  35% of sequence in fragments shorter than    9691 bp
  40% of sequence in fragments shorter than   11299 bp
  45% of sequence in fragments shorter than   13058 bp
  50% of sequence in fragments shorter than   15004 bp
  55% of sequence in fragments shorter than   17203 bp
  60% of sequence in fragments shorter than   19688 bp
  65% of sequence in fragments shorter than   22565 bp
  70% of sequence in fragments shorter than   25864 bp
  75% of sequence in fragments shorter than   29933 bp
  80% of sequence in fragments shorter than   35080 bp
  85% of sequence in fragments shorter than   41735 bp
  90% of sequence in fragments shorter than   51756 bp
  95% of sequence in fragments shorter than   72058 bp

# BsrBI.SexAI (CCGCTC+ACCWGGT) double digest, 532 thousand fragments (235523+297900 sites), 5591 bp average fragment length
   5% of sequence in fragments shorter than    1893 bp
  10% of sequence in fragments shorter than    3000 bp
  15% of sequence in fragments shorter than    3993 bp
  20% of sequence in fragments shorter than    4954 bp
  25% of sequence in fragments shorter than    5913 bp
  30% of sequence in fragments shorter than    6880 bp
  35% of sequence in fragments shorter than    7871 bp
  40% of sequence in fragments shorter than    8904 bp
  45% of sequence in fragments shorter than   10013 bp
  50% of sequence in fragments shorter than   11213 bp
  55% of sequence in fragments shorter than   12501 bp
  60% of sequence in fragments shorter than   13906 bp
  65% of sequence in fragments shorter than   15502 bp
  70% of sequence in fragments shorter than   17316 bp
  75% of sequence in fragments shorter than   19503 bp
  80% of sequence in fragments shorter than   22115 bp
  85% of sequence in fragments shorter than   25520 bp
  90% of sequence in fragments shorter than   30529 bp
  95% of sequence in fragments shorter than   40014 bp

# ClaI.EcoRV (ATCGAT+GATATC) double digest, 532 thousand fragments (86477+446473 sites), 5596 bp average fragment length
   5% of sequence in fragments shorter than    1931 bp
  10% of sequence in fragments shorter than    2966 bp
  15% of sequence in fragments shorter than    3880 bp
  20% of sequence in fragments shorter than    4737 bp
  25% of sequence in fragments shorter than    5565 bp
  30% of sequence in fragments shorter than    6407 bp
  35% of sequence in fragments shorter than    7274 bp
  40% of sequence in fragments shorter than    8177 bp
  45% of sequence in fragments shorter than    9121 bp
  50% of sequence in fragments shorter than   10117 bp
  55% of sequence in fragments shorter than   11213 bp
  60% of sequence in fragments shorter than   12431 bp
  65% of sequence in fragments shorter than   13766 bp
  70% of sequence in fragments shorter than   15308 bp
  75% of sequence in fragments shorter than   17155 bp
  80% of sequence in fragments shorter than   19480 bp
  85% of sequence in fragments shorter than   22580 bp
  90% of sequence in fragments shorter than   27174 bp
  95% of sequence in fragments shorter than   36450 bp

# SnaBI.SpeI (TACGTA+ACTAGT) double digest, 532 thousand fragments (132299+400286 sites), 5599 bp average fragment length
   5% of sequence in fragments shorter than    1934 bp
  10% of sequence in fragments shorter than    2915 bp
  15% of sequence in fragments shorter than    3802 bp
  20% of sequence in fragments shorter than    4627 bp
  25% of sequence in fragments shorter than    5444 bp
  30% of sequence in fragments shorter than    6255 bp
  35% of sequence in fragments shorter than    7076 bp
  40% of sequence in fragments shorter than    7936 bp
  45% of sequence in fragments shorter than    8855 bp
  50% of sequence in fragments shorter than    9831 bp
  55% of sequence in fragments shorter than   10902 bp
  60% of sequence in fragments shorter than   12082 bp
  65% of sequence in fragments shorter than   13428 bp
  70% of sequence in fragments shorter than   14996 bp
  75% of sequence in fragments shorter than   16852 bp
  80% of sequence in fragments shorter than   19158 bp
  85% of sequence in fragments shorter than   22309 bp
  90% of sequence in fragments shorter than   27229 bp
  95% of sequence in fragments shorter than   38195 bp

# AatII.ApaI (GACGTC+GGGCCC) double digest, 531 thousand fragments (71089+460339 sites), 5610 bp average fragment length
   5% of sequence in fragments shorter than    1750 bp
  10% of sequence in fragments shorter than    3019 bp
  15% of sequence in fragments shorter than    4257 bp
  20% of sequence in fragments shorter than    5515 bp
  25% of sequence in fragments shorter than    6809 bp
  30% of sequence in fragments shorter than    8162 bp
  35% of sequence in fragments shorter than    9565 bp
  40% of sequence in fragments shorter than   11060 bp
  45% of sequence in fragments shorter than   12653 bp
  50% of sequence in fragments shorter than   14407 bp
  55% of sequence in fragments shorter than   16352 bp
  60% of sequence in fragments shorter than   18525 bp
  65% of sequence in fragments shorter than   20995 bp
  70% of sequence in fragments shorter than   23856 bp
  75% of sequence in fragments shorter than   27247 bp
  80% of sequence in fragments shorter than   31525 bp
  85% of sequence in fragments shorter than   37148 bp
  90% of sequence in fragments shorter than   45408 bp
  95% of sequence in fragments shorter than   62259 bp

# KasI.SexAI (GGCGCC+ACCWGGT) double digest, 527 thousand fragments (230381+297900 sites), 5644 bp average fragment length
   5% of sequence in fragments shorter than    1912 bp
  10% of sequence in fragments shorter than    2998 bp
  15% of sequence in fragments shorter than    3964 bp
  20% of sequence in fragments shorter than    4890 bp
  25% of sequence in fragments shorter than    5815 bp
  30% of sequence in fragments shorter than    6742 bp
  35% of sequence in fragments shorter than    7702 bp
  40% of sequence in fragments shorter than    8696 bp
  45% of sequence in fragments shorter than    9756 bp
  50% of sequence in fragments shorter than   10896 bp
  55% of sequence in fragments shorter than   12142 bp
  60% of sequence in fragments shorter than   13522 bp
  65% of sequence in fragments shorter than   15086 bp
  70% of sequence in fragments shorter than   16876 bp
  75% of sequence in fragments shorter than   18996 bp
  80% of sequence in fragments shorter than   21572 bp
  85% of sequence in fragments shorter than   25010 bp
  90% of sequence in fragments shorter than   30177 bp
  95% of sequence in fragments shorter than   41106 bp

# ApaLI.SalI (GTGCAC+GTCGAC) double digest, 527 thousand fragments (496255+31396 sites), 5651 bp average fragment length
   5% of sequence in fragments shorter than    1994 bp
  10% of sequence in fragments shorter than    3031 bp
  15% of sequence in fragments shorter than    3930 bp
  20% of sequence in fragments shorter than    4778 bp
  25% of sequence in fragments shorter than    5614 bp
  30% of sequence in fragments shorter than    6407 bp
  35% of sequence in fragments shorter than    7248 bp
  40% of sequence in fragments shorter than    8138 bp
  45% of sequence in fragments shorter than    9063 bp
  50% of sequence in fragments shorter than   10028 bp
  55% of sequence in fragments shorter than   11097 bp
  60% of sequence in fragments shorter than   12276 bp
  65% of sequence in fragments shorter than   13591 bp
  70% of sequence in fragments shorter than   15082 bp
  75% of sequence in fragments shorter than   16860 bp
  80% of sequence in fragments shorter than   18987 bp
  85% of sequence in fragments shorter than   21840 bp
  90% of sequence in fragments shorter than   25922 bp
  95% of sequence in fragments shorter than   33867 bp

# ApaI.SacII (GGGCCC+CCGCGG) double digest, 526 thousand fragments (460339+66901 sites), 5654 bp average fragment length
   5% of sequence in fragments shorter than    1816 bp
  10% of sequence in fragments shorter than    3244 bp
  15% of sequence in fragments shorter than    4656 bp
  20% of sequence in fragments shorter than    6100 bp
  25% of sequence in fragments shorter than    7598 bp
  30% of sequence in fragments shorter than    9156 bp
  35% of sequence in fragments shorter than   10777 bp
  40% of sequence in fragments shorter than   12522 bp
  45% of sequence in fragments shorter than   14390 bp
  50% of sequence in fragments shorter than   16446 bp
  55% of sequence in fragments shorter than   18714 bp
  60% of sequence in fragments shorter than   21278 bp
  65% of sequence in fragments shorter than   24157 bp
  70% of sequence in fragments shorter than   27471 bp
  75% of sequence in fragments shorter than   31493 bp
  80% of sequence in fragments shorter than   36591 bp
  85% of sequence in fragments shorter than   43360 bp
  90% of sequence in fragments shorter than   53870 bp
  95% of sequence in fragments shorter than   75222 bp

# NaeI.SpeI (GCCGGC+ACTAGT) double digest, 525 thousand fragments (125692+400286 sites), 5669 bp average fragment length
   5% of sequence in fragments shorter than    2019 bp
  10% of sequence in fragments shorter than    3065 bp
  15% of sequence in fragments shorter than    3988 bp
  20% of sequence in fragments shorter than    4844 bp
  25% of sequence in fragments shorter than    5680 bp
  30% of sequence in fragments shorter than    6513 bp
  35% of sequence in fragments shorter than    7342 bp
  40% of sequence in fragments shorter than    8210 bp
  45% of sequence in fragments shorter than    9115 bp
  50% of sequence in fragments shorter than   10080 bp
  55% of sequence in fragments shorter than   11117 bp
  60% of sequence in fragments shorter than   12223 bp
  65% of sequence in fragments shorter than   13457 bp
  70% of sequence in fragments shorter than   14868 bp
  75% of sequence in fragments shorter than   16519 bp
  80% of sequence in fragments shorter than   18548 bp
  85% of sequence in fragments shorter than   21166 bp
  90% of sequence in fragments shorter than   24977 bp
  95% of sequence in fragments shorter than   32528 bp

# BamHI.PacI (GGATCC+TTAATTAA) double digest, 525 thousand fragments (365999+159455 sites), 5674 bp average fragment length
   5% of sequence in fragments shorter than    2054 bp
  10% of sequence in fragments shorter than    3076 bp
  15% of sequence in fragments shorter than    3970 bp
  20% of sequence in fragments shorter than    4808 bp
  25% of sequence in fragments shorter than    5611 bp
  30% of sequence in fragments shorter than    6418 bp
  35% of sequence in fragments shorter than    7229 bp
  40% of sequence in fragments shorter than    8066 bp
  45% of sequence in fragments shorter than    8951 bp
  50% of sequence in fragments shorter than    9887 bp
  55% of sequence in fragments shorter than   10894 bp
  60% of sequence in fragments shorter than   11983 bp
  65% of sequence in fragments shorter than   13201 bp
  70% of sequence in fragments shorter than   14598 bp
  75% of sequence in fragments shorter than   16222 bp
  80% of sequence in fragments shorter than   18192 bp
  85% of sequence in fragments shorter than   20764 bp
  90% of sequence in fragments shorter than   24503 bp
  95% of sequence in fragments shorter than   31753 bp

# SexAI.SwaI (ACCWGGT+ATTTAAAT) double digest, 524 thousand fragments (297900+226602 sites), 5684 bp average fragment length
   5% of sequence in fragments shorter than    1993 bp
  10% of sequence in fragments shorter than    3014 bp
  15% of sequence in fragments shorter than    3899 bp
  20% of sequence in fragments shorter than    4731 bp
  25% of sequence in fragments shorter than    5541 bp
  30% of sequence in fragments shorter than    6362 bp
  35% of sequence in fragments shorter than    7188 bp
  40% of sequence in fragments shorter than    8037 bp
  45% of sequence in fragments shorter than    8917 bp
  50% of sequence in fragments shorter than    9861 bp
  55% of sequence in fragments shorter than   10876 bp
  60% of sequence in fragments shorter than   11989 bp
  65% of sequence in fragments shorter than   13189 bp
  70% of sequence in fragments shorter than   14563 bp
  75% of sequence in fragments shorter than   16177 bp
  80% of sequence in fragments shorter than   18180 bp
  85% of sequence in fragments shorter than   20752 bp
  90% of sequence in fragments shorter than   24571 bp
  95% of sequence in fragments shorter than   32615 bp

# BamHI.Hpy99I (GGATCC+CGWCG) double digest, 523 thousand fragments (365999+157917 sites), 5692 bp average fragment length
   5% of sequence in fragments shorter than    1994 bp
  10% of sequence in fragments shorter than    3102 bp
  15% of sequence in fragments shorter than    4109 bp
  20% of sequence in fragments shorter than    5020 bp
  25% of sequence in fragments shorter than    5969 bp
  30% of sequence in fragments shorter than    6927 bp
  35% of sequence in fragments shorter than    7927 bp
  40% of sequence in fragments shorter than    8973 bp
  45% of sequence in fragments shorter than   10069 bp
  50% of sequence in fragments shorter than   11248 bp
  55% of sequence in fragments shorter than   12541 bp
  60% of sequence in fragments shorter than   13948 bp
  65% of sequence in fragments shorter than   15545 bp
  70% of sequence in fragments shorter than   17342 bp
  75% of sequence in fragments shorter than   19431 bp
  80% of sequence in fragments shorter than   22081 bp
  85% of sequence in fragments shorter than   25520 bp
  90% of sequence in fragments shorter than   30432 bp
  95% of sequence in fragments shorter than   39776 bp

# BsrBI.KpnI (CCGCTC+GGTACC) double digest, 523 thousand fragments (235523+287974 sites), 5697 bp average fragment length
   5% of sequence in fragments shorter than    1858 bp
  10% of sequence in fragments shorter than    3010 bp
  15% of sequence in fragments shorter than    4051 bp
  20% of sequence in fragments shorter than    5065 bp
  25% of sequence in fragments shorter than    6060 bp
  30% of sequence in fragments shorter than    7052 bp
  35% of sequence in fragments shorter than    8068 bp
  40% of sequence in fragments shorter than    9123 bp
  45% of sequence in fragments shorter than   10240 bp
  50% of sequence in fragments shorter than   11440 bp
  55% of sequence in fragments shorter than   12711 bp
  60% of sequence in fragments shorter than   14127 bp
  65% of sequence in fragments shorter than   15670 bp
  70% of sequence in fragments shorter than   17440 bp
  75% of sequence in fragments shorter than   19568 bp
  80% of sequence in fragments shorter than   22061 bp
  85% of sequence in fragments shorter than   25376 bp
  90% of sequence in fragments shorter than   30267 bp
  95% of sequence in fragments shorter than   39575 bp

# BsrBI.NheI (CCGCTC+GCTAGC) double digest, 522 thousand fragments (235523+287384 sites), 5703 bp average fragment length
   5% of sequence in fragments shorter than    1895 bp
  10% of sequence in fragments shorter than    2992 bp
  15% of sequence in fragments shorter than    4056 bp
  20% of sequence in fragments shorter than    5080 bp
  25% of sequence in fragments shorter than    6070 bp
  30% of sequence in fragments shorter than    7078 bp
  35% of sequence in fragments shorter than    8092 bp
  40% of sequence in fragments shorter than    9152 bp
  45% of sequence in fragments shorter than   10263 bp
  50% of sequence in fragments shorter than   11439 bp
  55% of sequence in fragments shorter than   12713 bp
  60% of sequence in fragments shorter than   14098 bp
  65% of sequence in fragments shorter than   15632 bp
  70% of sequence in fragments shorter than   17392 bp
  75% of sequence in fragments shorter than   19426 bp
  80% of sequence in fragments shorter than   21864 bp
  85% of sequence in fragments shorter than   25102 bp
  90% of sequence in fragments shorter than   29743 bp
  95% of sequence in fragments shorter than   38306 bp

# HpaI.SnaBI (GTTAAC+TACGTA) double digest, 521 thousand fragments (389418+132299 sites), 5715 bp average fragment length
   5% of sequence in fragments shorter than    1989 bp
  10% of sequence in fragments shorter than    3025 bp
  15% of sequence in fragments shorter than    3907 bp
  20% of sequence in fragments shorter than    4754 bp
  25% of sequence in fragments shorter than    5592 bp
  30% of sequence in fragments shorter than    6424 bp
  35% of sequence in fragments shorter than    7268 bp
  40% of sequence in fragments shorter than    8157 bp
  45% of sequence in fragments shorter than    9091 bp
  50% of sequence in fragments shorter than   10090 bp
  55% of sequence in fragments shorter than   11170 bp
  60% of sequence in fragments shorter than   12354 bp
  65% of sequence in fragments shorter than   13693 bp
  70% of sequence in fragments shorter than   15211 bp
  75% of sequence in fragments shorter than   17033 bp
  80% of sequence in fragments shorter than   19257 bp
  85% of sequence in fragments shorter than   22237 bp
  90% of sequence in fragments shorter than   26793 bp
  95% of sequence in fragments shorter than   36687 bp

# SpeI.XhoI (ACTAGT+CTCGAG) double digest, 521 thousand fragments (400286+121308 sites), 5717 bp average fragment length
   5% of sequence in fragments shorter than    2021 bp
  10% of sequence in fragments shorter than    3033 bp
  15% of sequence in fragments shorter than    3915 bp
  20% of sequence in fragments shorter than    4654 bp
  25% of sequence in fragments shorter than    5457 bp
  30% of sequence in fragments shorter than    6261 bp
  35% of sequence in fragments shorter than    7068 bp
  40% of sequence in fragments shorter than    7902 bp
  45% of sequence in fragments shorter than    8785 bp
  50% of sequence in fragments shorter than    9706 bp
  55% of sequence in fragments shorter than   10710 bp
  60% of sequence in fragments shorter than   11789 bp
  65% of sequence in fragments shorter than   12981 bp
  70% of sequence in fragments shorter than   14357 bp
  75% of sequence in fragments shorter than   15985 bp
  80% of sequence in fragments shorter than   17917 bp
  85% of sequence in fragments shorter than   20442 bp
  90% of sequence in fragments shorter than   24166 bp
  95% of sequence in fragments shorter than   31499 bp

# ApaI.PspXI (GGGCCC+VCTCGAGB) double digest, 519 thousand fragments (460339+59193 sites), 5739 bp average fragment length
   5% of sequence in fragments shorter than    1780 bp
  10% of sequence in fragments shorter than    3091 bp
  15% of sequence in fragments shorter than    4383 bp
  20% of sequence in fragments shorter than    5684 bp
  25% of sequence in fragments shorter than    7034 bp
  30% of sequence in fragments shorter than    8429 bp
  35% of sequence in fragments shorter than    9902 bp
  40% of sequence in fragments shorter than   11471 bp
  45% of sequence in fragments shorter than   13141 bp
  50% of sequence in fragments shorter than   14998 bp
  55% of sequence in fragments shorter than   16970 bp
  60% of sequence in fragments shorter than   19250 bp
  65% of sequence in fragments shorter than   21902 bp
  70% of sequence in fragments shorter than   24891 bp
  75% of sequence in fragments shorter than   28422 bp
  80% of sequence in fragments shorter than   32953 bp
  85% of sequence in fragments shorter than   39127 bp
  90% of sequence in fragments shorter than   48141 bp
  95% of sequence in fragments shorter than   67244 bp

# KasI.KpnI (GGCGCC+GGTACC) double digest, 518 thousand fragments (230381+287974 sites), 5752 bp average fragment length
   5% of sequence in fragments shorter than    1876 bp
  10% of sequence in fragments shorter than    3005 bp
  15% of sequence in fragments shorter than    3993 bp
  20% of sequence in fragments shorter than    4953 bp
  25% of sequence in fragments shorter than    5889 bp
  30% of sequence in fragments shorter than    6832 bp
  35% of sequence in fragments shorter than    7803 bp
  40% of sequence in fragments shorter than    8807 bp
  45% of sequence in fragments shorter than    9871 bp
  50% of sequence in fragments shorter than   11006 bp
  55% of sequence in fragments shorter than   12243 bp
  60% of sequence in fragments shorter than   13609 bp
  65% of sequence in fragments shorter than   15132 bp
  70% of sequence in fragments shorter than   16875 bp
  75% of sequence in fragments shorter than   18936 bp
  80% of sequence in fragments shorter than   21450 bp
  85% of sequence in fragments shorter than   24762 bp
  90% of sequence in fragments shorter than   29770 bp
  95% of sequence in fragments shorter than   40350 bp

# KasI.NheI (GGCGCC+GCTAGC) double digest, 517 thousand fragments (230381+287384 sites), 5759 bp average fragment length
   5% of sequence in fragments shorter than    1935 bp
  10% of sequence in fragments shorter than    2996 bp
  15% of sequence in fragments shorter than    4018 bp
  20% of sequence in fragments shorter than    4990 bp
  25% of sequence in fragments shorter than    5937 bp
  30% of sequence in fragments shorter than    6893 bp
  35% of sequence in fragments shorter than    7875 bp
  40% of sequence in fragments shorter than    8878 bp
  45% of sequence in fragments shorter than    9944 bp
  50% of sequence in fragments shorter than   11064 bp
  55% of sequence in fragments shorter than   12281 bp
  60% of sequence in fragments shorter than   13648 bp
  65% of sequence in fragments shorter than   15126 bp
  70% of sequence in fragments shorter than   16841 bp
  75% of sequence in fragments shorter than   18837 bp
  80% of sequence in fragments shorter than   21272 bp
  85% of sequence in fragments shorter than   24507 bp
  90% of sequence in fragments shorter than   29280 bp
  95% of sequence in fragments shorter than   38650 bp

# ApaLI.MluI (GTGCAC+ACGCGT) double digest, 517 thousand fragments (496255+21415 sites), 5760 bp average fragment length
   5% of sequence in fragments shorter than    2034 bp
  10% of sequence in fragments shorter than    3096 bp
  15% of sequence in fragments shorter than    4020 bp
  20% of sequence in fragments shorter than    4887 bp
  25% of sequence in fragments shorter than    5744 bp
  30% of sequence in fragments shorter than    6566 bp
  35% of sequence in fragments shorter than    7438 bp
  40% of sequence in fragments shorter than    8351 bp
  45% of sequence in fragments shorter than    9294 bp
  50% of sequence in fragments shorter than   10303 bp
  55% of sequence in fragments shorter than   11396 bp
  60% of sequence in fragments shorter than   12615 bp
  65% of sequence in fragments shorter than   13991 bp
  70% of sequence in fragments shorter than   15547 bp
  75% of sequence in fragments shorter than   17371 bp
  80% of sequence in fragments shorter than   19634 bp
  85% of sequence in fragments shorter than   22549 bp
  90% of sequence in fragments shorter than   26794 bp
  95% of sequence in fragments shorter than   35038 bp

# AatII.EcoRV (GACGTC+GATATC) double digest, 517 thousand fragments (71089+446473 sites), 5762 bp average fragment length
   5% of sequence in fragments shorter than    1998 bp
  10% of sequence in fragments shorter than    3065 bp
  15% of sequence in fragments shorter than    3982 bp
  20% of sequence in fragments shorter than    4841 bp
  25% of sequence in fragments shorter than    5679 bp
  30% of sequence in fragments shorter than    6526 bp
  35% of sequence in fragments shorter than    7384 bp
  40% of sequence in fragments shorter than    8275 bp
  45% of sequence in fragments shorter than    9198 bp
  50% of sequence in fragments shorter than   10180 bp
  55% of sequence in fragments shorter than   11245 bp
  60% of sequence in fragments shorter than   12409 bp
  65% of sequence in fragments shorter than   13697 bp
  70% of sequence in fragments shorter than   15155 bp
  75% of sequence in fragments shorter than   16871 bp
  80% of sequence in fragments shorter than   18930 bp
  85% of sequence in fragments shorter than   21639 bp
  90% of sequence in fragments shorter than   25573 bp
  95% of sequence in fragments shorter than   33005 bp

# HpaI.NaeI (GTTAAC+GCCGGC) double digest, 514 thousand fragments (389418+125692 sites), 5788 bp average fragment length
   5% of sequence in fragments shorter than    2061 bp
  10% of sequence in fragments shorter than    3151 bp
  15% of sequence in fragments shorter than    4088 bp
  20% of sequence in fragments shorter than    4970 bp
  25% of sequence in fragments shorter than    5834 bp
  30% of sequence in fragments shorter than    6698 bp
  35% of sequence in fragments shorter than    7568 bp
  40% of sequence in fragments shorter than    8478 bp
  45% of sequence in fragments shorter than    9423 bp
  50% of sequence in fragments shorter than   10408 bp
  55% of sequence in fragments shorter than   11508 bp
  60% of sequence in fragments shorter than   12676 bp
  65% of sequence in fragments shorter than   13970 bp
  70% of sequence in fragments shorter than   15442 bp
  75% of sequence in fragments shorter than   17184 bp
  80% of sequence in fragments shorter than   19277 bp
  85% of sequence in fragments shorter than   22062 bp
  90% of sequence in fragments shorter than   26131 bp
  95% of sequence in fragments shorter than   34506 bp

# KpnI.SwaI (GGTACC+ATTTAAAT) double digest, 514 thousand fragments (287974+226602 sites), 5794 bp average fragment length
   5% of sequence in fragments shorter than    1932 bp
  10% of sequence in fragments shorter than    2989 bp
  15% of sequence in fragments shorter than    3889 bp
  20% of sequence in fragments shorter than    4761 bp
  25% of sequence in fragments shorter than    5597 bp
  30% of sequence in fragments shorter than    6438 bp
  35% of sequence in fragments shorter than    7301 bp
  40% of sequence in fragments shorter than    8196 bp
  45% of sequence in fragments shorter than    9129 bp
  50% of sequence in fragments shorter than   10120 bp
  55% of sequence in fragments shorter than   11183 bp
  60% of sequence in fragments shorter than   12358 bp
  65% of sequence in fragments shorter than   13666 bp
  70% of sequence in fragments shorter than   15149 bp
  75% of sequence in fragments shorter than   16906 bp
  80% of sequence in fragments shorter than   19072 bp
  85% of sequence in fragments shorter than   21907 bp
  90% of sequence in fragments shorter than   26170 bp
  95% of sequence in fragments shorter than   35312 bp

# NheI.SwaI (GCTAGC+ATTTAAAT) double digest, 513 thousand fragments (287384+226602 sites), 5801 bp average fragment length
   5% of sequence in fragments shorter than    1952 bp
  10% of sequence in fragments shorter than    2947 bp
  15% of sequence in fragments shorter than    3894 bp
  20% of sequence in fragments shorter than    4786 bp
  25% of sequence in fragments shorter than    5661 bp
  30% of sequence in fragments shorter than    6535 bp
  35% of sequence in fragments shorter than    7433 bp
  40% of sequence in fragments shorter than    8351 bp
  45% of sequence in fragments shorter than    9320 bp
  50% of sequence in fragments shorter than   10342 bp
  55% of sequence in fragments shorter than   11474 bp
  60% of sequence in fragments shorter than   12676 bp
  65% of sequence in fragments shorter than   14057 bp
  70% of sequence in fragments shorter than   15650 bp
  75% of sequence in fragments shorter than   17544 bp
  80% of sequence in fragments shorter than   19868 bp
  85% of sequence in fragments shorter than   23020 bp
  90% of sequence in fragments shorter than   27800 bp
  95% of sequence in fragments shorter than   37982 bp

# AgeI.ApaI (ACCGGT+GGGCCC) double digest, 513 thousand fragments (53386+460339 sites), 5804 bp average fragment length
   5% of sequence in fragments shorter than    1820 bp
  10% of sequence in fragments shorter than    3132 bp
  15% of sequence in fragments shorter than    4422 bp
  20% of sequence in fragments shorter than    5701 bp
  25% of sequence in fragments shorter than    7019 bp
  30% of sequence in fragments shorter than    8383 bp
  35% of sequence in fragments shorter than    9802 bp
  40% of sequence in fragments shorter than   11308 bp
  45% of sequence in fragments shorter than   12931 bp
  50% of sequence in fragments shorter than   14693 bp
  55% of sequence in fragments shorter than   16600 bp
  60% of sequence in fragments shorter than   18760 bp
  65% of sequence in fragments shorter than   21195 bp
  70% of sequence in fragments shorter than   24015 bp
  75% of sequence in fragments shorter than   27396 bp
  80% of sequence in fragments shorter than   31631 bp
  85% of sequence in fragments shorter than   37293 bp
  90% of sequence in fragments shorter than   45958 bp
  95% of sequence in fragments shorter than   63747 bp

# EcoRV.SacII (GATATC+CCGCGG) double digest, 512 thousand fragments (446473+66901 sites), 5809 bp average fragment length
   5% of sequence in fragments shorter than    2058 bp
  10% of sequence in fragments shorter than    3196 bp
  15% of sequence in fragments shorter than    4179 bp
  20% of sequence in fragments shorter than    5091 bp
  25% of sequence in fragments shorter than    5975 bp
  30% of sequence in fragments shorter than    6866 bp
  35% of sequence in fragments shorter than    7769 bp
  40% of sequence in fragments shorter than    8701 bp
  45% of sequence in fragments shorter than    9677 bp
  50% of sequence in fragments shorter than   10728 bp
  55% of sequence in fragments shorter than   11848 bp
  60% of sequence in fragments shorter than   13075 bp
  65% of sequence in fragments shorter than   14440 bp
  70% of sequence in fragments shorter than   15986 bp
  75% of sequence in fragments shorter than   17782 bp
  80% of sequence in fragments shorter than   19950 bp
  85% of sequence in fragments shorter than   22810 bp
  90% of sequence in fragments shorter than   26984 bp
  95% of sequence in fragments shorter than   34795 bp

# ApaLI.SgrAI (GTGCAC+CRCCGGYG) double digest, 511 thousand fragments (496255+15270 sites), 5830 bp average fragment length
   5% of sequence in fragments shorter than    2057 bp
  10% of sequence in fragments shorter than    3131 bp
  15% of sequence in fragments shorter than    4067 bp
  20% of sequence in fragments shorter than    4945 bp
  25% of sequence in fragments shorter than    5812 bp
  30% of sequence in fragments shorter than    6645 bp
  35% of sequence in fragments shorter than    7533 bp
  40% of sequence in fragments shorter than    8450 bp
  45% of sequence in fragments shorter than    9401 bp
  50% of sequence in fragments shorter than   10427 bp
  55% of sequence in fragments shorter than   11530 bp
  60% of sequence in fragments shorter than   12768 bp
  65% of sequence in fragments shorter than   14143 bp
  70% of sequence in fragments shorter than   15706 bp
  75% of sequence in fragments shorter than   17563 bp
  80% of sequence in fragments shorter than   19856 bp
  85% of sequence in fragments shorter than   22790 bp
  90% of sequence in fragments shorter than   27113 bp
  95% of sequence in fragments shorter than   35503 bp

# ApaLI.NruI (GTGCAC+TCGCGA) double digest, 510 thousand fragments (496255+14872 sites), 5834 bp average fragment length
   5% of sequence in fragments shorter than    2057 bp
  10% of sequence in fragments shorter than    3125 bp
  15% of sequence in fragments shorter than    4057 bp
  20% of sequence in fragments shorter than    4932 bp
  25% of sequence in fragments shorter than    5792 bp
  30% of sequence in fragments shorter than    6623 bp
  35% of sequence in fragments shorter than    7505 bp
  40% of sequence in fragments shorter than    8420 bp
  45% of sequence in fragments shorter than    9364 bp
  50% of sequence in fragments shorter than   10383 bp
  55% of sequence in fragments shorter than   11480 bp
  60% of sequence in fragments shorter than   12699 bp
  65% of sequence in fragments shorter than   14071 bp
  70% of sequence in fragments shorter than   15630 bp
  75% of sequence in fragments shorter than   17448 bp
  80% of sequence in fragments shorter than   19725 bp
  85% of sequence in fragments shorter than   22658 bp
  90% of sequence in fragments shorter than   26900 bp
  95% of sequence in fragments shorter than   35212 bp

# HpaI.XhoI (GTTAAC+CTCGAG) double digest, 510 thousand fragments (389418+121308 sites), 5838 bp average fragment length
   5% of sequence in fragments shorter than    2049 bp
  10% of sequence in fragments shorter than    3113 bp
  15% of sequence in fragments shorter than    4013 bp
  20% of sequence in fragments shorter than    4860 bp
  25% of sequence in fragments shorter than    5672 bp
  30% of sequence in fragments shorter than    6490 bp
  35% of sequence in fragments shorter than    7315 bp
  40% of sequence in fragments shorter than    8180 bp
  45% of sequence in fragments shorter than    9070 bp
  50% of sequence in fragments shorter than   10021 bp
  55% of sequence in fragments shorter than   11029 bp
  60% of sequence in fragments shorter than   12135 bp
  65% of sequence in fragments shorter than   13355 bp
  70% of sequence in fragments shorter than   14772 bp
  75% of sequence in fragments shorter than   16396 bp
  80% of sequence in fragments shorter than   18388 bp
  85% of sequence in fragments shorter than   20936 bp
  90% of sequence in fragments shorter than   24738 bp
  95% of sequence in fragments shorter than   32411 bp

# ApaLI.PvuI (GTGCAC+CGATCG) double digest, 510 thousand fragments (496255+14186 sites), 5842 bp average fragment length
   5% of sequence in fragments shorter than    2049 bp
  10% of sequence in fragments shorter than    3126 bp
  15% of sequence in fragments shorter than    4061 bp
  20% of sequence in fragments shorter than    4939 bp
  25% of sequence in fragments shorter than    5799 bp
  30% of sequence in fragments shorter than    6631 bp
  35% of sequence in fragments shorter than    7514 bp
  40% of sequence in fragments shorter than    8431 bp
  45% of sequence in fragments shorter than    9376 bp
  50% of sequence in fragments shorter than   10393 bp
  55% of sequence in fragments shorter than   11492 bp
  60% of sequence in fragments shorter than   12709 bp
  65% of sequence in fragments shorter than   14086 bp
  70% of sequence in fragments shorter than   15652 bp
  75% of sequence in fragments shorter than   17472 bp
  80% of sequence in fragments shorter than   19736 bp
  85% of sequence in fragments shorter than   22647 bp
  90% of sequence in fragments shorter than   26872 bp
  95% of sequence in fragments shorter than   35060 bp

# ApaLI.FseI (GTGCAC+GGCCGGCC) double digest, 509 thousand fragments (496255+13662 sites), 5848 bp average fragment length
   5% of sequence in fragments shorter than    2058 bp
  10% of sequence in fragments shorter than    3136 bp
  15% of sequence in fragments shorter than    4071 bp
  20% of sequence in fragments shorter than    4953 bp
  25% of sequence in fragments shorter than    5817 bp
  30% of sequence in fragments shorter than    6656 bp
  35% of sequence in fragments shorter than    7545 bp
  40% of sequence in fragments shorter than    8464 bp
  45% of sequence in fragments shorter than    9422 bp
  50% of sequence in fragments shorter than   10455 bp
  55% of sequence in fragments shorter than   11560 bp
  60% of sequence in fragments shorter than   12795 bp
  65% of sequence in fragments shorter than   14186 bp
  70% of sequence in fragments shorter than   15761 bp
  75% of sequence in fragments shorter than   17621 bp
  80% of sequence in fragments shorter than   19950 bp
  85% of sequence in fragments shorter than   22910 bp
  90% of sequence in fragments shorter than   27239 bp
  95% of sequence in fragments shorter than   35706 bp

# SapI.SnaBI (GCTCTTC+TACGTA) double digest, 509 thousand fragments (377161+132299 sites), 5853 bp average fragment length
   5% of sequence in fragments shorter than    2043 bp
  10% of sequence in fragments shorter than    3112 bp
  15% of sequence in fragments shorter than    4037 bp
  20% of sequence in fragments shorter than    4898 bp
  25% of sequence in fragments shorter than    5742 bp
  30% of sequence in fragments shorter than    6580 bp
  35% of sequence in fragments shorter than    7439 bp
  40% of sequence in fragments shorter than    8320 bp
  45% of sequence in fragments shorter than    9240 bp
  50% of sequence in fragments shorter than   10213 bp
  55% of sequence in fragments shorter than   11268 bp
  60% of sequence in fragments shorter than   12422 bp
  65% of sequence in fragments shorter than   13702 bp
  70% of sequence in fragments shorter than   15141 bp
  75% of sequence in fragments shorter than   16818 bp
  80% of sequence in fragments shorter than   18867 bp
  85% of sequence in fragments shorter than   21585 bp
  90% of sequence in fragments shorter than   25431 bp
  95% of sequence in fragments shorter than   33150 bp

# SmaI.SnaBI (CCCGGG+TACGTA) double digest, 508 thousand fragments (376940+132299 sites), 5855 bp average fragment length
   5% of sequence in fragments shorter than    1918 bp
  10% of sequence in fragments shorter than    3071 bp
  15% of sequence in fragments shorter than    4136 bp
  20% of sequence in fragments shorter than    5191 bp
  25% of sequence in fragments shorter than    6263 bp
  30% of sequence in fragments shorter than    7352 bp
  35% of sequence in fragments shorter than    8507 bp
  40% of sequence in fragments shorter than    9717 bp
  45% of sequence in fragments shorter than   11019 bp
  50% of sequence in fragments shorter than   12429 bp
  55% of sequence in fragments shorter than   13995 bp
  60% of sequence in fragments shorter than   15718 bp
  65% of sequence in fragments shorter than   17708 bp
  70% of sequence in fragments shorter than   19946 bp
  75% of sequence in fragments shorter than   22677 bp
  80% of sequence in fragments shorter than   26031 bp
  85% of sequence in fragments shorter than   30449 bp
  90% of sequence in fragments shorter than   37062 bp
  95% of sequence in fragments shorter than   50341 bp

# AclI.BstEII (AACGTT+GGTNACC) double digest, 508 thousand fragments (172706+335865 sites), 5864 bp average fragment length
   5% of sequence in fragments shorter than    2052 bp
  10% of sequence in fragments shorter than    3120 bp
  15% of sequence in fragments shorter than    4019 bp
  20% of sequence in fragments shorter than    4897 bp
  25% of sequence in fragments shorter than    5736 bp
  30% of sequence in fragments shorter than    6592 bp
  35% of sequence in fragments shorter than    7451 bp
  40% of sequence in fragments shorter than    8338 bp
  45% of sequence in fragments shorter than    9276 bp
  50% of sequence in fragments shorter than   10259 bp
  55% of sequence in fragments shorter than   11324 bp
  60% of sequence in fragments shorter than   12462 bp
  65% of sequence in fragments shorter than   13732 bp
  70% of sequence in fragments shorter than   15172 bp
  75% of sequence in fragments shorter than   16893 bp
  80% of sequence in fragments shorter than   18965 bp
  85% of sequence in fragments shorter than   21562 bp
  90% of sequence in fragments shorter than   25428 bp
  95% of sequence in fragments shorter than   32712 bp

# ApaLI.NotI (GTGCAC+GCGGCCGC) double digest, 505 thousand fragments (496255+9795 sites), 5893 bp average fragment length
   5% of sequence in fragments shorter than    2079 bp
  10% of sequence in fragments shorter than    3167 bp
  15% of sequence in fragments shorter than    4109 bp
  20% of sequence in fragments shorter than    4997 bp
  25% of sequence in fragments shorter than    5863 bp
  30% of sequence in fragments shorter than    6708 bp
  35% of sequence in fragments shorter than    7599 bp
  40% of sequence in fragments shorter than    8521 bp
  45% of sequence in fragments shorter than    9481 bp
  50% of sequence in fragments shorter than   10524 bp
  55% of sequence in fragments shorter than   11638 bp
  60% of sequence in fragments shorter than   12880 bp
  65% of sequence in fragments shorter than   14267 bp
  70% of sequence in fragments shorter than   15853 bp
  75% of sequence in fragments shorter than   17723 bp
  80% of sequence in fragments shorter than   20045 bp
  85% of sequence in fragments shorter than   22987 bp
  90% of sequence in fragments shorter than   27332 bp
  95% of sequence in fragments shorter than   35846 bp

# ApaLI.RsrII (GTGCAC+CGGWCCG) double digest, 505 thousand fragments (496255+9764 sites), 5893 bp average fragment length
   5% of sequence in fragments shorter than    2078 bp
  10% of sequence in fragments shorter than    3162 bp
  15% of sequence in fragments shorter than    4102 bp
  20% of sequence in fragments shorter than    4987 bp
  25% of sequence in fragments shorter than    5850 bp
  30% of sequence in fragments shorter than    6693 bp
  35% of sequence in fragments shorter than    7584 bp
  40% of sequence in fragments shorter than    8502 bp
  45% of sequence in fragments shorter than    9460 bp
  50% of sequence in fragments shorter than   10494 bp
  55% of sequence in fragments shorter than   11606 bp
  60% of sequence in fragments shorter than   12843 bp
  65% of sequence in fragments shorter than   14234 bp
  70% of sequence in fragments shorter than   15808 bp
  75% of sequence in fragments shorter than   17673 bp
  80% of sequence in fragments shorter than   19984 bp
  85% of sequence in fragments shorter than   22926 bp
  90% of sequence in fragments shorter than   27234 bp
  95% of sequence in fragments shorter than   35652 bp

# EcoRV.PspXI (GATATC+VCTCGAGB) double digest, 505 thousand fragments (446473+59193 sites), 5898 bp average fragment length
   5% of sequence in fragments shorter than    2043 bp
  10% of sequence in fragments shorter than    3132 bp
  15% of sequence in fragments shorter than    4082 bp
  20% of sequence in fragments shorter than    4959 bp
  25% of sequence in fragments shorter than    5812 bp
  30% of sequence in fragments shorter than    6673 bp
  35% of sequence in fragments shorter than    7551 bp
  40% of sequence in fragments shorter than    8461 bp
  45% of sequence in fragments shorter than    9397 bp
  50% of sequence in fragments shorter than   10413 bp
  55% of sequence in fragments shorter than   11503 bp
  60% of sequence in fragments shorter than   12684 bp
  65% of sequence in fragments shorter than   13993 bp
  70% of sequence in fragments shorter than   15479 bp
  75% of sequence in fragments shorter than   17217 bp
  80% of sequence in fragments shorter than   19354 bp
  85% of sequence in fragments shorter than   22133 bp
  90% of sequence in fragments shorter than   26158 bp
  95% of sequence in fragments shorter than   33706 bp

# NaeI.SapI (GCCGGC+GCTCTTC) double digest, 502 thousand fragments (125692+377161 sites), 5929 bp average fragment length
   5% of sequence in fragments shorter than    2037 bp
  10% of sequence in fragments shorter than    3222 bp
  15% of sequence in fragments shorter than    4259 bp
  20% of sequence in fragments shorter than    5237 bp
  25% of sequence in fragments shorter than    6195 bp
  30% of sequence in fragments shorter than    7176 bp
  35% of sequence in fragments shorter than    8174 bp
  40% of sequence in fragments shorter than    9204 bp
  45% of sequence in fragments shorter than   10289 bp
  50% of sequence in fragments shorter than   11476 bp
  55% of sequence in fragments shorter than   12737 bp
  60% of sequence in fragments shorter than   14126 bp
  65% of sequence in fragments shorter than   15675 bp
  70% of sequence in fragments shorter than   17451 bp
  75% of sequence in fragments shorter than   19546 bp
  80% of sequence in fragments shorter than   22136 bp
  85% of sequence in fragments shorter than   25583 bp
  90% of sequence in fragments shorter than   30742 bp
  95% of sequence in fragments shorter than   41399 bp

# NaeI.SmaI (GCCGGC+CCCGGG) double digest, 502 thousand fragments (125692+376940 sites), 5931 bp average fragment length
   5% of sequence in fragments shorter than    1911 bp
  10% of sequence in fragments shorter than    3252 bp
  15% of sequence in fragments shorter than    4564 bp
  20% of sequence in fragments shorter than    5930 bp
  25% of sequence in fragments shorter than    7368 bp
  30% of sequence in fragments shorter than    8915 bp
  35% of sequence in fragments shorter than   10584 bp
  40% of sequence in fragments shorter than   12387 bp
  45% of sequence in fragments shorter than   14431 bp
  50% of sequence in fragments shorter than   16703 bp
  55% of sequence in fragments shorter than   19277 bp
  60% of sequence in fragments shorter than   22211 bp
  65% of sequence in fragments shorter than   25593 bp
  70% of sequence in fragments shorter than   29648 bp
  75% of sequence in fragments shorter than   34488 bp
  80% of sequence in fragments shorter than   40584 bp
  85% of sequence in fragments shorter than   48663 bp
  90% of sequence in fragments shorter than   61674 bp
  95% of sequence in fragments shorter than   90168 bp

# ApaI.PmeI (GGGCCC+GTTTAAAC) double digest, 502 thousand fragments (460339+42094 sites), 5934 bp average fragment length
   5% of sequence in fragments shorter than    1875 bp
  10% of sequence in fragments shorter than    3204 bp
  15% of sequence in fragments shorter than    4486 bp
  20% of sequence in fragments shorter than    5777 bp
  25% of sequence in fragments shorter than    7103 bp
  30% of sequence in fragments shorter than    8450 bp
  35% of sequence in fragments shorter than    9862 bp
  40% of sequence in fragments shorter than   11341 bp
  45% of sequence in fragments shorter than   12945 bp
  50% of sequence in fragments shorter than   14669 bp
  55% of sequence in fragments shorter than   16533 bp
  60% of sequence in fragments shorter than   18596 bp
  65% of sequence in fragments shorter than   20953 bp
  70% of sequence in fragments shorter than   23681 bp
  75% of sequence in fragments shorter than   26899 bp
  80% of sequence in fragments shorter than   30975 bp
  85% of sequence in fragments shorter than   36287 bp
  90% of sequence in fragments shorter than   43982 bp
  95% of sequence in fragments shorter than   60079 bp

# ApaLI.AscI (GTGCAC+GGCGCGCC) double digest, 500 thousand fragments (496255+4751 sites), 5952 bp average fragment length
   5% of sequence in fragments shorter than    2100 bp
  10% of sequence in fragments shorter than    3194 bp
  15% of sequence in fragments shorter than    4144 bp
  20% of sequence in fragments shorter than    5038 bp
  25% of sequence in fragments shorter than    5905 bp
  30% of sequence in fragments shorter than    6756 bp
  35% of sequence in fragments shorter than    7656 bp
  40% of sequence in fragments shorter than    8581 bp
  45% of sequence in fragments shorter than    9547 bp
  50% of sequence in fragments shorter than   10591 bp
  55% of sequence in fragments shorter than   11706 bp
  60% of sequence in fragments shorter than   12954 bp
  65% of sequence in fragments shorter than   14342 bp
  70% of sequence in fragments shorter than   15931 bp
  75% of sequence in fragments shorter than   17788 bp
  80% of sequence in fragments shorter than   20124 bp
  85% of sequence in fragments shorter than   23082 bp
  90% of sequence in fragments shorter than   27461 bp
  95% of sequence in fragments shorter than   35916 bp

# AgeI.EcoRV (ACCGGT+GATATC) double digest, 499 thousand fragments (53386+446473 sites), 5966 bp average fragment length
   5% of sequence in fragments shorter than    2042 bp
  10% of sequence in fragments shorter than    3149 bp
  15% of sequence in fragments shorter than    4120 bp
  20% of sequence in fragments shorter than    5020 bp
  25% of sequence in fragments shorter than    5901 bp
  30% of sequence in fragments shorter than    6789 bp
  35% of sequence in fragments shorter than    7690 bp
  40% of sequence in fragments shorter than    8626 bp
  45% of sequence in fragments shorter than    9612 bp
  50% of sequence in fragments shorter than   10670 bp
  55% of sequence in fragments shorter than   11791 bp
  60% of sequence in fragments shorter than   13035 bp
  65% of sequence in fragments shorter than   14405 bp
  70% of sequence in fragments shorter than   15985 bp
  75% of sequence in fragments shorter than   17843 bp
  80% of sequence in fragments shorter than   20128 bp
  85% of sequence in fragments shorter than   23123 bp
  90% of sequence in fragments shorter than   27619 bp
  95% of sequence in fragments shorter than   36170 bp

# SapI.XhoI (GCTCTTC+CTCGAG) double digest, 498 thousand fragments (377161+121308 sites), 5982 bp average fragment length
   5% of sequence in fragments shorter than    2069 bp
  10% of sequence in fragments shorter than    3200 bp
  15% of sequence in fragments shorter than    4181 bp
  20% of sequence in fragments shorter than    5090 bp
  25% of sequence in fragments shorter than    5981 bp
  30% of sequence in fragments shorter than    6888 bp
  35% of sequence in fragments shorter than    7805 bp
  40% of sequence in fragments shorter than    8758 bp
  45% of sequence in fragments shorter than    9756 bp
  50% of sequence in fragments shorter than   10821 bp
  55% of sequence in fragments shorter than   11975 bp
  60% of sequence in fragments shorter than   13245 bp
  65% of sequence in fragments shorter than   14661 bp
  70% of sequence in fragments shorter than   16281 bp
  75% of sequence in fragments shorter than   18170 bp
  80% of sequence in fragments shorter than   20563 bp
  85% of sequence in fragments shorter than   23613 bp
  90% of sequence in fragments shorter than   28205 bp
  95% of sequence in fragments shorter than   37911 bp

# BamHI.SnaBI (GGATCC+TACGTA) double digest, 497 thousand fragments (365999+132299 sites), 5984 bp average fragment length
   5% of sequence in fragments shorter than    2088 bp
  10% of sequence in fragments shorter than    3167 bp
  15% of sequence in fragments shorter than    4122 bp
  20% of sequence in fragments shorter than    5009 bp
  25% of sequence in fragments shorter than    5861 bp
  30% of sequence in fragments shorter than    6704 bp
  35% of sequence in fragments shorter than    7582 bp
  40% of sequence in fragments shorter than    8477 bp
  45% of sequence in fragments shorter than    9424 bp
  50% of sequence in fragments shorter than   10417 bp
  55% of sequence in fragments shorter than   11491 bp
  60% of sequence in fragments shorter than   12679 bp
  65% of sequence in fragments shorter than   13969 bp
  70% of sequence in fragments shorter than   15439 bp
  75% of sequence in fragments shorter than   17140 bp
  80% of sequence in fragments shorter than   19245 bp
  85% of sequence in fragments shorter than   21983 bp
  90% of sequence in fragments shorter than   25905 bp
  95% of sequence in fragments shorter than   33212 bp

# SmaI.XhoI (CCCGGG+CTCGAG) double digest, 497 thousand fragments (376940+121308 sites), 5984 bp average fragment length
   5% of sequence in fragments shorter than    1898 bp
  10% of sequence in fragments shorter than    3117 bp
  15% of sequence in fragments shorter than    4284 bp
  20% of sequence in fragments shorter than    5443 bp
  25% of sequence in fragments shorter than    6658 bp
  30% of sequence in fragments shorter than    7938 bp
  35% of sequence in fragments shorter than    9292 bp
  40% of sequence in fragments shorter than   10753 bp
  45% of sequence in fragments shorter than   12364 bp
  50% of sequence in fragments shorter than   14106 bp
  55% of sequence in fragments shorter than   16059 bp
  60% of sequence in fragments shorter than   18310 bp
  65% of sequence in fragments shorter than   20892 bp
  70% of sequence in fragments shorter than   23855 bp
  75% of sequence in fragments shorter than   27597 bp
  80% of sequence in fragments shorter than   32144 bp
  85% of sequence in fragments shorter than   38290 bp
  90% of sequence in fragments shorter than   47462 bp
  95% of sequence in fragments shorter than   66938 bp

# BstEII.PacI (GGTNACC+TTAATTAA) double digest, 495 thousand fragments (335865+159455 sites), 6019 bp average fragment length
   5% of sequence in fragments shorter than    2172 bp
  10% of sequence in fragments shorter than    3282 bp
  15% of sequence in fragments shorter than    4229 bp
  20% of sequence in fragments shorter than    5111 bp
  25% of sequence in fragments shorter than    5968 bp
  30% of sequence in fragments shorter than    6821 bp
  35% of sequence in fragments shorter than    7686 bp
  40% of sequence in fragments shorter than    8576 bp
  45% of sequence in fragments shorter than    9513 bp
  50% of sequence in fragments shorter than   10524 bp
  55% of sequence in fragments shorter than   11607 bp
  60% of sequence in fragments shorter than   12775 bp
  65% of sequence in fragments shorter than   14089 bp
  70% of sequence in fragments shorter than   15563 bp
  75% of sequence in fragments shorter than   17305 bp
  80% of sequence in fragments shorter than   19431 bp
  85% of sequence in fragments shorter than   22217 bp
  90% of sequence in fragments shorter than   26358 bp
  95% of sequence in fragments shorter than   34915 bp

# BstEII.Hpy99I (GGTNACC+CGWCG) double digest, 493 thousand fragments (335865+157917 sites), 6039 bp average fragment length
   5% of sequence in fragments shorter than    2075 bp
  10% of sequence in fragments shorter than    3313 bp
  15% of sequence in fragments shorter than    4408 bp
  20% of sequence in fragments shorter than    5432 bp
  25% of sequence in fragments shorter than    6457 bp
  30% of sequence in fragments shorter than    7494 bp
  35% of sequence in fragments shorter than    8548 bp
  40% of sequence in fragments shorter than    9655 bp
  45% of sequence in fragments shorter than   10822 bp
  50% of sequence in fragments shorter than   12086 bp
  55% of sequence in fragments shorter than   13461 bp
  60% of sequence in fragments shorter than   14963 bp
  65% of sequence in fragments shorter than   16650 bp
  70% of sequence in fragments shorter than   18576 bp
  75% of sequence in fragments shorter than   20869 bp
  80% of sequence in fragments shorter than   23673 bp
  85% of sequence in fragments shorter than   27425 bp
  90% of sequence in fragments shorter than   32868 bp
  95% of sequence in fragments shorter than   43657 bp

# ApaI.SalI (GGGCCC+GTCGAC) double digest, 491 thousand fragments (460339+31396 sites), 6063 bp average fragment length
   5% of sequence in fragments shorter than    1888 bp
  10% of sequence in fragments shorter than    3278 bp
  15% of sequence in fragments shorter than    4634 bp
  20% of sequence in fragments shorter than    6011 bp
  25% of sequence in fragments shorter than    7433 bp
  30% of sequence in fragments shorter than    8902 bp
  35% of sequence in fragments shorter than   10442 bp
  40% of sequence in fragments shorter than   12071 bp
  45% of sequence in fragments shorter than   13801 bp
  50% of sequence in fragments shorter than   15743 bp
  55% of sequence in fragments shorter than   17828 bp
  60% of sequence in fragments shorter than   20205 bp
  65% of sequence in fragments shorter than   22921 bp
  70% of sequence in fragments shorter than   26008 bp
  75% of sequence in fragments shorter than   29788 bp
  80% of sequence in fragments shorter than   34447 bp
  85% of sequence in fragments shorter than   40860 bp
  90% of sequence in fragments shorter than   50229 bp
  95% of sequence in fragments shorter than   69056 bp

# BamHI.NaeI (GGATCC+GCCGGC) double digest, 491 thousand fragments (365999+125692 sites), 6064 bp average fragment length
   5% of sequence in fragments shorter than    2086 bp
  10% of sequence in fragments shorter than    3272 bp
  15% of sequence in fragments shorter than    4353 bp
  20% of sequence in fragments shorter than    5380 bp
  25% of sequence in fragments shorter than    6397 bp
  30% of sequence in fragments shorter than    7440 bp
  35% of sequence in fragments shorter than    8501 bp
  40% of sequence in fragments shorter than    9618 bp
  45% of sequence in fragments shorter than   10785 bp
  50% of sequence in fragments shorter than   12051 bp
  55% of sequence in fragments shorter than   13415 bp
  60% of sequence in fragments shorter than   14931 bp
  65% of sequence in fragments shorter than   16624 bp
  70% of sequence in fragments shorter than   18584 bp
  75% of sequence in fragments shorter than   20893 bp
  80% of sequence in fragments shorter than   23803 bp
  85% of sequence in fragments shorter than   27581 bp
  90% of sequence in fragments shorter than   33120 bp
  95% of sequence in fragments shorter than   43817 bp

# EcoRV.PmeI (GATATC+GTTTAAAC) double digest, 488 thousand fragments (446473+42094 sites), 6104 bp average fragment length
   5% of sequence in fragments shorter than    2083 bp
  10% of sequence in fragments shorter than    3207 bp
  15% of sequence in fragments shorter than    4183 bp
  20% of sequence in fragments shorter than    5098 bp
  25% of sequence in fragments shorter than    5999 bp
  30% of sequence in fragments shorter than    6912 bp
  35% of sequence in fragments shorter than    7843 bp
  40% of sequence in fragments shorter than    8807 bp
  45% of sequence in fragments shorter than    9831 bp
  50% of sequence in fragments shorter than   10953 bp
  55% of sequence in fragments shorter than   12132 bp
  60% of sequence in fragments shorter than   13464 bp
  65% of sequence in fragments shorter than   14963 bp
  70% of sequence in fragments shorter than   16684 bp
  75% of sequence in fragments shorter than   18746 bp
  80% of sequence in fragments shorter than   21417 bp
  85% of sequence in fragments shorter than   24943 bp
  90% of sequence in fragments shorter than   30244 bp
  95% of sequence in fragments shorter than   41472 bp

# BamHI.XhoI (GGATCC+CTCGAG) double digest, 486 thousand fragments (365999+121308 sites), 6119 bp average fragment length
   5% of sequence in fragments shorter than    2093 bp
  10% of sequence in fragments shorter than    3218 bp
  15% of sequence in fragments shorter than    4120 bp
  20% of sequence in fragments shorter than    5062 bp
  25% of sequence in fragments shorter than    5997 bp
  30% of sequence in fragments shorter than    6928 bp
  35% of sequence in fragments shorter than    7897 bp
  40% of sequence in fragments shorter than    8903 bp
  45% of sequence in fragments shorter than    9961 bp
  50% of sequence in fragments shorter than   11090 bp
  55% of sequence in fragments shorter than   12326 bp
  60% of sequence in fragments shorter than   13683 bp
  65% of sequence in fragments shorter than   15194 bp
  70% of sequence in fragments shorter than   16909 bp
  75% of sequence in fragments shorter than   18936 bp
  80% of sequence in fragments shorter than   21452 bp
  85% of sequence in fragments shorter than   24749 bp
  90% of sequence in fragments shorter than   29530 bp
  95% of sequence in fragments shorter than   39226 bp

# ClaI.SpeI (ATCGAT+ACTAGT) double digest, 486 thousand fragments (86477+400286 sites), 6126 bp average fragment length
   5% of sequence in fragments shorter than    2082 bp
  10% of sequence in fragments shorter than    3178 bp
  15% of sequence in fragments shorter than    4123 bp
  20% of sequence in fragments shorter than    5010 bp
  25% of sequence in fragments shorter than    5874 bp
  30% of sequence in fragments shorter than    6750 bp
  35% of sequence in fragments shorter than    7638 bp
  40% of sequence in fragments shorter than    8562 bp
  45% of sequence in fragments shorter than    9549 bp
  50% of sequence in fragments shorter than   10611 bp
  55% of sequence in fragments shorter than   11748 bp
  60% of sequence in fragments shorter than   13010 bp
  65% of sequence in fragments shorter than   14426 bp
  70% of sequence in fragments shorter than   16073 bp
  75% of sequence in fragments shorter than   18007 bp
  80% of sequence in fragments shorter than   20412 bp
  85% of sequence in fragments shorter than   23644 bp
  90% of sequence in fragments shorter than   28719 bp
  95% of sequence in fragments shorter than   39811 bp

# ApaI.MluI (GGGCCC+ACGCGT) double digest, 481 thousand fragments (460339+21415 sites), 6189 bp average fragment length
   5% of sequence in fragments shorter than    1918 bp
  10% of sequence in fragments shorter than    3351 bp
  15% of sequence in fragments shorter than    4762 bp
  20% of sequence in fragments shorter than    6209 bp
  25% of sequence in fragments shorter than    7699 bp
  30% of sequence in fragments shorter than    9240 bp
  35% of sequence in fragments shorter than   10848 bp
  40% of sequence in fragments shorter than   12585 bp
  45% of sequence in fragments shorter than   14441 bp
  50% of sequence in fragments shorter than   16468 bp
  55% of sequence in fragments shorter than   18700 bp
  60% of sequence in fragments shorter than   21228 bp
  65% of sequence in fragments shorter than   24100 bp
  70% of sequence in fragments shorter than   27424 bp
  75% of sequence in fragments shorter than   31447 bp
  80% of sequence in fragments shorter than   36450 bp
  85% of sequence in fragments shorter than   43189 bp
  90% of sequence in fragments shorter than   53462 bp
  95% of sequence in fragments shorter than   74010 bp

# EcoRV.SalI (GATATC+GTCGAC) double digest, 477 thousand fragments (446473+31396 sites), 6241 bp average fragment length
   5% of sequence in fragments shorter than    2149 bp
  10% of sequence in fragments shorter than    3296 bp
  15% of sequence in fragments shorter than    4300 bp
  20% of sequence in fragments shorter than    5237 bp
  25% of sequence in fragments shorter than    6162 bp
  30% of sequence in fragments shorter than    7084 bp
  35% of sequence in fragments shorter than    8041 bp
  40% of sequence in fragments shorter than    9019 bp
  45% of sequence in fragments shorter than   10052 bp
  50% of sequence in fragments shorter than   11172 bp
  55% of sequence in fragments shorter than   12370 bp
  60% of sequence in fragments shorter than   13686 bp
  65% of sequence in fragments shorter than   15182 bp
  70% of sequence in fragments shorter than   16883 bp
  75% of sequence in fragments shorter than   18898 bp
  80% of sequence in fragments shorter than   21400 bp
  85% of sequence in fragments shorter than   24777 bp
  90% of sequence in fragments shorter than   29701 bp
  95% of sequence in fragments shorter than   39599 bp

# ClaI.HpaI (ATCGAT+GTTAAC) double digest, 475 thousand fragments (86477+389418 sites), 6266 bp average fragment length
   5% of sequence in fragments shorter than    2185 bp
  10% of sequence in fragments shorter than    3312 bp
  15% of sequence in fragments shorter than    4274 bp
  20% of sequence in fragments shorter than    5181 bp
  25% of sequence in fragments shorter than    6071 bp
  30% of sequence in fragments shorter than    6957 bp
  35% of sequence in fragments shorter than    7877 bp
  40% of sequence in fragments shorter than    8821 bp
  45% of sequence in fragments shorter than    9812 bp
  50% of sequence in fragments shorter than   10877 bp
  55% of sequence in fragments shorter than   12031 bp
  60% of sequence in fragments shorter than   13291 bp
  65% of sequence in fragments shorter than   14692 bp
  70% of sequence in fragments shorter than   16313 bp
  75% of sequence in fragments shorter than   18230 bp
  80% of sequence in fragments shorter than   20570 bp
  85% of sequence in fragments shorter than   23704 bp
  90% of sequence in fragments shorter than   28366 bp
  95% of sequence in fragments shorter than   38309 bp

# ApaI.SgrAI (GGGCCC+CRCCGGYG) double digest, 475 thousand fragments (460339+15270 sites), 6269 bp average fragment length
   5% of sequence in fragments shorter than    1950 bp
  10% of sequence in fragments shorter than    3419 bp
  15% of sequence in fragments shorter than    4876 bp
  20% of sequence in fragments shorter than    6358 bp
  25% of sequence in fragments shorter than    7891 bp
  30% of sequence in fragments shorter than    9485 bp
  35% of sequence in fragments shorter than   11141 bp
  40% of sequence in fragments shorter than   12924 bp
  45% of sequence in fragments shorter than   14847 bp
  50% of sequence in fragments shorter than   16913 bp
  55% of sequence in fragments shorter than   19227 bp
  60% of sequence in fragments shorter than   21881 bp
  65% of sequence in fragments shorter than   24804 bp
  70% of sequence in fragments shorter than   28226 bp
  75% of sequence in fragments shorter than   32434 bp
  80% of sequence in fragments shorter than   37690 bp
  85% of sequence in fragments shorter than   44545 bp
  90% of sequence in fragments shorter than   55221 bp
  95% of sequence in fragments shorter than   76722 bp

# ApaI.NruI (GGGCCC+TCGCGA) double digest, 474 thousand fragments (460339+14872 sites), 6274 bp average fragment length
   5% of sequence in fragments shorter than    1948 bp
  10% of sequence in fragments shorter than    3399 bp
  15% of sequence in fragments shorter than    4832 bp
  20% of sequence in fragments shorter than    6285 bp
  25% of sequence in fragments shorter than    7790 bp
  30% of sequence in fragments shorter than    9352 bp
  35% of sequence in fragments shorter than   10982 bp
  40% of sequence in fragments shorter than   12730 bp
  45% of sequence in fragments shorter than   14622 bp
  50% of sequence in fragments shorter than   16645 bp
  55% of sequence in fragments shorter than   18900 bp
  60% of sequence in fragments shorter than   21482 bp
  65% of sequence in fragments shorter than   24355 bp
  70% of sequence in fragments shorter than   27688 bp
  75% of sequence in fragments shorter than   31729 bp
  80% of sequence in fragments shorter than   36804 bp
  85% of sequence in fragments shorter than   43613 bp
  90% of sequence in fragments shorter than   54173 bp
  95% of sequence in fragments shorter than   74496 bp

# ApaI.PvuI (GGGCCC+CGATCG) double digest, 474 thousand fragments (460339+14186 sites), 6283 bp average fragment length
   5% of sequence in fragments shorter than    1933 bp
  10% of sequence in fragments shorter than    3388 bp
  15% of sequence in fragments shorter than    4823 bp
  20% of sequence in fragments shorter than    6283 bp
  25% of sequence in fragments shorter than    7789 bp
  30% of sequence in fragments shorter than    9355 bp
  35% of sequence in fragments shorter than   10990 bp
  40% of sequence in fragments shorter than   12733 bp
  45% of sequence in fragments shorter than   14623 bp
  50% of sequence in fragments shorter than   16664 bp
  55% of sequence in fragments shorter than   18946 bp
  60% of sequence in fragments shorter than   21499 bp
  65% of sequence in fragments shorter than   24376 bp
  70% of sequence in fragments shorter than   27730 bp
  75% of sequence in fragments shorter than   31779 bp
  80% of sequence in fragments shorter than   36794 bp
  85% of sequence in fragments shorter than   43613 bp
  90% of sequence in fragments shorter than   53879 bp
  95% of sequence in fragments shorter than   73458 bp

# ApaI.FseI (GGGCCC+GGCCGGCC) double digest, 473 thousand fragments (460339+13662 sites), 6290 bp average fragment length
   5% of sequence in fragments shorter than    1955 bp
  10% of sequence in fragments shorter than    3429 bp
  15% of sequence in fragments shorter than    4890 bp
  20% of sequence in fragments shorter than    6377 bp
  25% of sequence in fragments shorter than    7916 bp
  30% of sequence in fragments shorter than    9517 bp
  35% of sequence in fragments shorter than   11177 bp
  40% of sequence in fragments shorter than   12968 bp
  45% of sequence in fragments shorter than   14909 bp
  50% of sequence in fragments shorter than   16991 bp
  55% of sequence in fragments shorter than   19315 bp
  60% of sequence in fragments shorter than   21974 bp
  65% of sequence in fragments shorter than   24920 bp
  70% of sequence in fragments shorter than   28358 bp
  75% of sequence in fragments shorter than   32575 bp
  80% of sequence in fragments shorter than   37835 bp
  85% of sequence in fragments shorter than   44818 bp
  90% of sequence in fragments shorter than   55585 bp
  95% of sequence in fragments shorter than   77433 bp

# AatII.SpeI (GACGTC+ACTAGT) double digest, 470 thousand fragments (71089+400286 sites), 6327 bp average fragment length
   5% of sequence in fragments shorter than    2202 bp
  10% of sequence in fragments shorter than    3331 bp
  15% of sequence in fragments shorter than    4311 bp
  20% of sequence in fragments shorter than    5228 bp
  25% of sequence in fragments shorter than    6118 bp
  30% of sequence in fragments shorter than    7002 bp
  35% of sequence in fragments shorter than    7894 bp
  40% of sequence in fragments shorter than    8831 bp
  45% of sequence in fragments shorter than    9805 bp
  50% of sequence in fragments shorter than   10857 bp
  55% of sequence in fragments shorter than   11964 bp
  60% of sequence in fragments shorter than   13191 bp
  65% of sequence in fragments shorter than   14578 bp
  70% of sequence in fragments shorter than   16149 bp
  75% of sequence in fragments shorter than   17965 bp
  80% of sequence in fragments shorter than   20197 bp
  85% of sequence in fragments shorter than   23070 bp
  90% of sequence in fragments shorter than   27292 bp
  95% of sequence in fragments shorter than   35713 bp

# AclI.SexAI (AACGTT+ACCWGGT) double digest, 470 thousand fragments (172706+297900 sites), 6337 bp average fragment length
   5% of sequence in fragments shorter than    2183 bp
  10% of sequence in fragments shorter than    3344 bp
  15% of sequence in fragments shorter than    4327 bp
  20% of sequence in fragments shorter than    5258 bp
  25% of sequence in fragments shorter than    6179 bp
  30% of sequence in fragments shorter than    7086 bp
  35% of sequence in fragments shorter than    8001 bp
  40% of sequence in fragments shorter than    8949 bp
  45% of sequence in fragments shorter than    9936 bp
  50% of sequence in fragments shorter than   10979 bp
  55% of sequence in fragments shorter than   12088 bp
  60% of sequence in fragments shorter than   13330 bp
  65% of sequence in fragments shorter than   14703 bp
  70% of sequence in fragments shorter than   16200 bp
  75% of sequence in fragments shorter than   17980 bp
  80% of sequence in fragments shorter than   20149 bp
  85% of sequence in fragments shorter than   22888 bp
  90% of sequence in fragments shorter than   26874 bp
  95% of sequence in fragments shorter than   34322 bp

# ApaI.NotI (GGGCCC+GCGGCCGC) double digest, 469 thousand fragments (460339+9795 sites), 6342 bp average fragment length
   5% of sequence in fragments shorter than    1977 bp
  10% of sequence in fragments shorter than    3469 bp
  15% of sequence in fragments shorter than    4947 bp
  20% of sequence in fragments shorter than    6444 bp
  25% of sequence in fragments shorter than    7995 bp
  30% of sequence in fragments shorter than    9615 bp
  35% of sequence in fragments shorter than   11295 bp
  40% of sequence in fragments shorter than   13096 bp
  45% of sequence in fragments shorter than   15075 bp
  50% of sequence in fragments shorter than   17157 bp
  55% of sequence in fragments shorter than   19513 bp
  60% of sequence in fragments shorter than   22211 bp
  65% of sequence in fragments shorter than   25160 bp
  70% of sequence in fragments shorter than   28636 bp
  75% of sequence in fragments shorter than   32919 bp
  80% of sequence in fragments shorter than   38192 bp
  85% of sequence in fragments shorter than   45207 bp
  90% of sequence in fragments shorter than   56035 bp
  95% of sequence in fragments shorter than   78246 bp

# ApaI.RsrII (GGGCCC+CGGWCCG) double digest, 469 thousand fragments (460339+9764 sites), 6342 bp average fragment length
   5% of sequence in fragments shorter than    1970 bp
  10% of sequence in fragments shorter than    3450 bp
  15% of sequence in fragments shorter than    4921 bp
  20% of sequence in fragments shorter than    6410 bp
  25% of sequence in fragments shorter than    7947 bp
  30% of sequence in fragments shorter than    9561 bp
  35% of sequence in fragments shorter than   11227 bp
  40% of sequence in fragments shorter than   13015 bp
  45% of sequence in fragments shorter than   14965 bp
  50% of sequence in fragments shorter than   17053 bp
  55% of sequence in fragments shorter than   19376 bp
  60% of sequence in fragments shorter than   22042 bp
  65% of sequence in fragments shorter than   25003 bp
  70% of sequence in fragments shorter than   28433 bp
  75% of sequence in fragments shorter than   32680 bp
  80% of sequence in fragments shorter than   37942 bp
  85% of sequence in fragments shorter than   44902 bp
  90% of sequence in fragments shorter than   55606 bp
  95% of sequence in fragments shorter than   77352 bp

# BstEII.SnaBI (GGTNACC+TACGTA) double digest, 467 thousand fragments (335865+132299 sites), 6369 bp average fragment length
   5% of sequence in fragments shorter than    2253 bp
  10% of sequence in fragments shorter than    3411 bp
  15% of sequence in fragments shorter than    4405 bp
  20% of sequence in fragments shorter than    5336 bp
  25% of sequence in fragments shorter than    6256 bp
  30% of sequence in fragments shorter than    7158 bp
  35% of sequence in fragments shorter than    8095 bp
  40% of sequence in fragments shorter than    9052 bp
  45% of sequence in fragments shorter than   10073 bp
  50% of sequence in fragments shorter than   11138 bp
  55% of sequence in fragments shorter than   12277 bp
  60% of sequence in fragments shorter than   13534 bp
  65% of sequence in fragments shorter than   14917 bp
  70% of sequence in fragments shorter than   16510 bp
  75% of sequence in fragments shorter than   18356 bp
  80% of sequence in fragments shorter than   20659 bp
  85% of sequence in fragments shorter than   23542 bp
  90% of sequence in fragments shorter than   27766 bp
  95% of sequence in fragments shorter than   36456 bp

# EcoRV.MluI (GATATC+ACGCGT) double digest, 467 thousand fragments (446473+21415 sites), 6374 bp average fragment length
   5% of sequence in fragments shorter than    2183 bp
  10% of sequence in fragments shorter than    3378 bp
  15% of sequence in fragments shorter than    4402 bp
  20% of sequence in fragments shorter than    5363 bp
  25% of sequence in fragments shorter than    6306 bp
  30% of sequence in fragments shorter than    7250 bp
  35% of sequence in fragments shorter than    8223 bp
  40% of sequence in fragments shorter than    9217 bp
  45% of sequence in fragments shorter than   10274 bp
  50% of sequence in fragments shorter than   11410 bp
  55% of sequence in fragments shorter than   12624 bp
  60% of sequence in fragments shorter than   13969 bp
  65% of sequence in fragments shorter than   15475 bp
  70% of sequence in fragments shorter than   17186 bp
  75% of sequence in fragments shorter than   19213 bp
  80% of sequence in fragments shorter than   21740 bp
  85% of sequence in fragments shorter than   25050 bp
  90% of sequence in fragments shorter than   30003 bp
  95% of sequence in fragments shorter than   39575 bp

# SacII.SpeI (CCGCGG+ACTAGT) double digest, 466 thousand fragments (66901+400286 sites), 6383 bp average fragment length
   5% of sequence in fragments shorter than    2274 bp
  10% of sequence in fragments shorter than    3488 bp
  15% of sequence in fragments shorter than    4535 bp
  20% of sequence in fragments shorter than    5512 bp
  25% of sequence in fragments shorter than    6461 bp
  30% of sequence in fragments shorter than    7393 bp
  35% of sequence in fragments shorter than    8354 bp
  40% of sequence in fragments shorter than    9352 bp
  45% of sequence in fragments shorter than   10396 bp
  50% of sequence in fragments shorter than   11513 bp
  55% of sequence in fragments shorter than   12692 bp
  60% of sequence in fragments shorter than   14013 bp
  65% of sequence in fragments shorter than   15507 bp
  70% of sequence in fragments shorter than   17173 bp
  75% of sequence in fragments shorter than   19135 bp
  80% of sequence in fragments shorter than   21530 bp
  85% of sequence in fragments shorter than   24684 bp
  90% of sequence in fragments shorter than   29405 bp
  95% of sequence in fragments shorter than   38921 bp

# BsrBI.KasI (CCGCTC+GGCGCC) double digest, 465 thousand fragments (235523+230381 sites), 6401 bp average fragment length
   5% of sequence in fragments shorter than    2164 bp
  10% of sequence in fragments shorter than    3628 bp
  15% of sequence in fragments shorter than    5047 bp
  20% of sequence in fragments shorter than    6465 bp
  25% of sequence in fragments shorter than    7912 bp
  30% of sequence in fragments shorter than    9445 bp
  35% of sequence in fragments shorter than   11072 bp
  40% of sequence in fragments shorter than   12830 bp
  45% of sequence in fragments shorter than   14729 bp
  50% of sequence in fragments shorter than   16825 bp
  55% of sequence in fragments shorter than   19127 bp
  60% of sequence in fragments shorter than   21728 bp
  65% of sequence in fragments shorter than   24655 bp
  70% of sequence in fragments shorter than   28171 bp
  75% of sequence in fragments shorter than   32270 bp
  80% of sequence in fragments shorter than   37453 bp
  85% of sequence in fragments shorter than   44149 bp
  90% of sequence in fragments shorter than   53861 bp
  95% of sequence in fragments shorter than   72221 bp

# ApaI.AscI (GGGCCC+GGCGCGCC) double digest, 464 thousand fragments (460339+4751 sites), 6411 bp average fragment length
   5% of sequence in fragments shorter than    1992 bp
  10% of sequence in fragments shorter than    3487 bp
  15% of sequence in fragments shorter than    4964 bp
  20% of sequence in fragments shorter than    6466 bp
  25% of sequence in fragments shorter than    8025 bp
  30% of sequence in fragments shorter than    9644 bp
  35% of sequence in fragments shorter than   11332 bp
  40% of sequence in fragments shorter than   13133 bp
  45% of sequence in fragments shorter than   15118 bp
  50% of sequence in fragments shorter than   17202 bp
  55% of sequence in fragments shorter than   19557 bp
  60% of sequence in fragments shorter than   22251 bp
  65% of sequence in fragments shorter than   25228 bp
  70% of sequence in fragments shorter than   28723 bp
  75% of sequence in fragments shorter than   33014 bp
  80% of sequence in fragments shorter than   38346 bp
  85% of sequence in fragments shorter than   45361 bp
  90% of sequence in fragments shorter than   56199 bp
  95% of sequence in fragments shorter than   78308 bp

# ClaI.SapI (ATCGAT+GCTCTTC) double digest, 463 thousand fragments (86477+377161 sites), 6432 bp average fragment length
   5% of sequence in fragments shorter than    2219 bp
  10% of sequence in fragments shorter than    3412 bp
  15% of sequence in fragments shorter than    4441 bp
  20% of sequence in fragments shorter than    5396 bp
  25% of sequence in fragments shorter than    6329 bp
  30% of sequence in fragments shorter than    7267 bp
  35% of sequence in fragments shorter than    8212 bp
  40% of sequence in fragments shorter than    9203 bp
  45% of sequence in fragments shorter than   10227 bp
  50% of sequence in fragments shorter than   11334 bp
  55% of sequence in fragments shorter than   12509 bp
  60% of sequence in fragments shorter than   13809 bp
  65% of sequence in fragments shorter than   15229 bp
  70% of sequence in fragments shorter than   16865 bp
  75% of sequence in fragments shorter than   18798 bp
  80% of sequence in fragments shorter than   21186 bp
  85% of sequence in fragments shorter than   24237 bp
  90% of sequence in fragments shorter than   28781 bp
  95% of sequence in fragments shorter than   38294 bp

# ClaI.SmaI (ATCGAT+CCCGGG) double digest, 463 thousand fragments (86477+376940 sites), 6434 bp average fragment length
   5% of sequence in fragments shorter than    2074 bp
  10% of sequence in fragments shorter than    3367 bp
  15% of sequence in fragments shorter than    4553 bp
  20% of sequence in fragments shorter than    5743 bp
  25% of sequence in fragments shorter than    6967 bp
  30% of sequence in fragments shorter than    8236 bp
  35% of sequence in fragments shorter than    9577 bp
  40% of sequence in fragments shorter than   11025 bp
  45% of sequence in fragments shorter than   12564 bp
  50% of sequence in fragments shorter than   14291 bp
  55% of sequence in fragments shorter than   16161 bp
  60% of sequence in fragments shorter than   18232 bp
  65% of sequence in fragments shorter than   20662 bp
  70% of sequence in fragments shorter than   23368 bp
  75% of sequence in fragments shorter than   26746 bp
  80% of sequence in fragments shorter than   30905 bp
  85% of sequence in fragments shorter than   36402 bp
  90% of sequence in fragments shorter than   44610 bp
  95% of sequence in fragments shorter than   62268 bp

# BsrBI.SwaI (CCGCTC+ATTTAAAT) double digest, 461 thousand fragments (235523+226602 sites), 6454 bp average fragment length
   5% of sequence in fragments shorter than    2289 bp
  10% of sequence in fragments shorter than    3567 bp
  15% of sequence in fragments shorter than    4683 bp
  20% of sequence in fragments shorter than    5732 bp
  25% of sequence in fragments shorter than    6772 bp
  30% of sequence in fragments shorter than    7806 bp
  35% of sequence in fragments shorter than    8861 bp
  40% of sequence in fragments shorter than    9956 bp
  45% of sequence in fragments shorter than   11105 bp
  50% of sequence in fragments shorter than   12294 bp
  55% of sequence in fragments shorter than   13594 bp
  60% of sequence in fragments shorter than   14999 bp
  65% of sequence in fragments shorter than   16586 bp
  70% of sequence in fragments shorter than   18368 bp
  75% of sequence in fragments shorter than   20441 bp
  80% of sequence in fragments shorter than   22912 bp
  85% of sequence in fragments shorter than   26299 bp
  90% of sequence in fragments shorter than   31090 bp
  95% of sequence in fragments shorter than   40223 bp

# EcoRV.SgrAI (GATATC+CRCCGGYG) double digest, 461 thousand fragments (446473+15270 sites), 6459 bp average fragment length
   5% of sequence in fragments shorter than    2212 bp
  10% of sequence in fragments shorter than    3420 bp
  15% of sequence in fragments shorter than    4460 bp
  20% of sequence in fragments shorter than    5430 bp
  25% of sequence in fragments shorter than    6387 bp
  30% of sequence in fragments shorter than    7350 bp
  35% of sequence in fragments shorter than    8337 bp
  40% of sequence in fragments shorter than    9352 bp
  45% of sequence in fragments shorter than   10433 bp
  50% of sequence in fragments shorter than   11591 bp
  55% of sequence in fragments shorter than   12828 bp
  60% of sequence in fragments shorter than   14198 bp
  65% of sequence in fragments shorter than   15737 bp
  70% of sequence in fragments shorter than   17515 bp
  75% of sequence in fragments shorter than   19588 bp
  80% of sequence in fragments shorter than   22189 bp
  85% of sequence in fragments shorter than   25591 bp
  90% of sequence in fragments shorter than   30731 bp
  95% of sequence in fragments shorter than   40855 bp

# BstEII.NaeI (GGTNACC+GCCGGC) double digest, 461 thousand fragments (335865+125692 sites), 6460 bp average fragment length
   5% of sequence in fragments shorter than    2224 bp
  10% of sequence in fragments shorter than    3525 bp
  15% of sequence in fragments shorter than    4691 bp
  20% of sequence in fragments shorter than    5787 bp
  25% of sequence in fragments shorter than    6878 bp
  30% of sequence in fragments shorter than    7975 bp
  35% of sequence in fragments shorter than    9122 bp
  40% of sequence in fragments shorter than   10309 bp
  45% of sequence in fragments shorter than   11567 bp
  50% of sequence in fragments shorter than   12908 bp
  55% of sequence in fragments shorter than   14388 bp
  60% of sequence in fragments shorter than   15982 bp
  65% of sequence in fragments shorter than   17799 bp
  70% of sequence in fragments shorter than   19874 bp
  75% of sequence in fragments shorter than   22357 bp
  80% of sequence in fragments shorter than   25398 bp
  85% of sequence in fragments shorter than   29558 bp
  90% of sequence in fragments shorter than   35613 bp
  95% of sequence in fragments shorter than   48533 bp

# EcoRV.NruI (GATATC+TCGCGA) double digest, 460 thousand fragments (446473+14872 sites), 6465 bp average fragment length
   5% of sequence in fragments shorter than    2209 bp
  10% of sequence in fragments shorter than    3417 bp
  15% of sequence in fragments shorter than    4453 bp
  20% of sequence in fragments shorter than    5426 bp
  25% of sequence in fragments shorter than    6384 bp
  30% of sequence in fragments shorter than    7343 bp
  35% of sequence in fragments shorter than    8334 bp
  40% of sequence in fragments shorter than    9349 bp
  45% of sequence in fragments shorter than   10435 bp
  50% of sequence in fragments shorter than   11600 bp
  55% of sequence in fragments shorter than   12855 bp
  60% of sequence in fragments shorter than   14234 bp
  65% of sequence in fragments shorter than   15791 bp
  70% of sequence in fragments shorter than   17590 bp
  75% of sequence in fragments shorter than   19699 bp
  80% of sequence in fragments shorter than   22321 bp
  85% of sequence in fragments shorter than   25844 bp
  90% of sequence in fragments shorter than   31060 bp
  95% of sequence in fragments shorter than   41337 bp

# AclI.KpnI (AACGTT+GGTACC) double digest, 460 thousand fragments (172706+287974 sites), 6474 bp average fragment length
   5% of sequence in fragments shorter than    2105 bp
  10% of sequence in fragments shorter than    3349 bp
  15% of sequence in fragments shorter than    4413 bp
  20% of sequence in fragments shorter than    5425 bp
  25% of sequence in fragments shorter than    6398 bp
  30% of sequence in fragments shorter than    7367 bp
  35% of sequence in fragments shorter than    8362 bp
  40% of sequence in fragments shorter than    9371 bp
  45% of sequence in fragments shorter than   10411 bp
  50% of sequence in fragments shorter than   11522 bp
  55% of sequence in fragments shorter than   12720 bp
  60% of sequence in fragments shorter than   14011 bp
  65% of sequence in fragments shorter than   15441 bp
  70% of sequence in fragments shorter than   17039 bp
  75% of sequence in fragments shorter than   18926 bp
  80% of sequence in fragments shorter than   21190 bp
  85% of sequence in fragments shorter than   24128 bp
  90% of sequence in fragments shorter than   28326 bp
  95% of sequence in fragments shorter than   36033 bp

# EcoRV.PvuI (GATATC+CGATCG) double digest, 460 thousand fragments (446473+14186 sites), 6474 bp average fragment length
   5% of sequence in fragments shorter than    2215 bp
  10% of sequence in fragments shorter than    3427 bp
  15% of sequence in fragments shorter than    4470 bp
  20% of sequence in fragments shorter than    5453 bp
  25% of sequence in fragments shorter than    6415 bp
  30% of sequence in fragments shorter than    7386 bp
  35% of sequence in fragments shorter than    8383 bp
  40% of sequence in fragments shorter than    9407 bp
  45% of sequence in fragments shorter than   10503 bp
  50% of sequence in fragments shorter than   11679 bp
  55% of sequence in fragments shorter than   12942 bp
  60% of sequence in fragments shorter than   14335 bp
  65% of sequence in fragments shorter than   15901 bp
  70% of sequence in fragments shorter than   17712 bp
  75% of sequence in fragments shorter than   19855 bp
  80% of sequence in fragments shorter than   22540 bp
  85% of sequence in fragments shorter than   26136 bp
  90% of sequence in fragments shorter than   31426 bp
  95% of sequence in fragments shorter than   42169 bp

# AatII.HpaI (GACGTC+GTTAAC) double digest, 460 thousand fragments (71089+389418 sites), 6475 bp average fragment length
   5% of sequence in fragments shorter than    2283 bp
  10% of sequence in fragments shorter than    3447 bp
  15% of sequence in fragments shorter than    4440 bp
  20% of sequence in fragments shorter than    5376 bp
  25% of sequence in fragments shorter than    6292 bp
  30% of sequence in fragments shorter than    7211 bp
  35% of sequence in fragments shorter than    8149 bp
  40% of sequence in fragments shorter than    9108 bp
  45% of sequence in fragments shorter than   10109 bp
  50% of sequence in fragments shorter than   11180 bp
  55% of sequence in fragments shorter than   12335 bp
  60% of sequence in fragments shorter than   13589 bp
  65% of sequence in fragments shorter than   14966 bp
  70% of sequence in fragments shorter than   16537 bp
  75% of sequence in fragments shorter than   18392 bp
  80% of sequence in fragments shorter than   20591 bp
  85% of sequence in fragments shorter than   23524 bp
  90% of sequence in fragments shorter than   27754 bp
  95% of sequence in fragments shorter than   36467 bp

# EcoRV.FseI (GATATC+GGCCGGCC) double digest, 459 thousand fragments (446473+13662 sites), 6482 bp average fragment length
   5% of sequence in fragments shorter than    2222 bp
  10% of sequence in fragments shorter than    3437 bp
  15% of sequence in fragments shorter than    4478 bp
  20% of sequence in fragments shorter than    5454 bp
  25% of sequence in fragments shorter than    6413 bp
  30% of sequence in fragments shorter than    7379 bp
  35% of sequence in fragments shorter than    8365 bp
  40% of sequence in fragments shorter than    9378 bp
  45% of sequence in fragments shorter than   10458 bp
  50% of sequence in fragments shorter than   11620 bp
  55% of sequence in fragments shorter than   12867 bp
  60% of sequence in fragments shorter than   14223 bp
  65% of sequence in fragments shorter than   15754 bp
  70% of sequence in fragments shorter than   17514 bp
  75% of sequence in fragments shorter than   19581 bp
  80% of sequence in fragments shorter than   22164 bp
  85% of sequence in fragments shorter than   25503 bp
  90% of sequence in fragments shorter than   30570 bp
  95% of sequence in fragments shorter than   40323 bp

# AclI.NheI (AACGTT+GCTAGC) double digest, 459 thousand fragments (172706+287384 sites), 6482 bp average fragment length
   5% of sequence in fragments shorter than    2101 bp
  10% of sequence in fragments shorter than    3332 bp
  15% of sequence in fragments shorter than    4457 bp
  20% of sequence in fragments shorter than    5484 bp
  25% of sequence in fragments shorter than    6492 bp
  30% of sequence in fragments shorter than    7489 bp
  35% of sequence in fragments shorter than    8496 bp
  40% of sequence in fragments shorter than    9521 bp
  45% of sequence in fragments shorter than   10586 bp
  50% of sequence in fragments shorter than   11713 bp
  55% of sequence in fragments shorter than   12914 bp
  60% of sequence in fragments shorter than   14238 bp
  65% of sequence in fragments shorter than   15686 bp
  70% of sequence in fragments shorter than   17344 bp
  75% of sequence in fragments shorter than   19273 bp
  80% of sequence in fragments shorter than   21610 bp
  85% of sequence in fragments shorter than   24652 bp
  90% of sequence in fragments shorter than   28899 bp
  95% of sequence in fragments shorter than   37029 bp

# PspXI.SpeI (VCTCGAGB+ACTAGT) double digest, 459 thousand fragments (59193+400286 sites), 6490 bp average fragment length
   5% of sequence in fragments shorter than    2255 bp
  10% of sequence in fragments shorter than    3419 bp
  15% of sequence in fragments shorter than    4422 bp
  20% of sequence in fragments shorter than    5356 bp
  25% of sequence in fragments shorter than    6260 bp
  30% of sequence in fragments shorter than    7152 bp
  35% of sequence in fragments shorter than    8071 bp
  40% of sequence in fragments shorter than    9019 bp
  45% of sequence in fragments shorter than   10025 bp
  50% of sequence in fragments shorter than   11099 bp
  55% of sequence in fragments shorter than   12234 bp
  60% of sequence in fragments shorter than   13491 bp
  65% of sequence in fragments shorter than   14914 bp
  70% of sequence in fragments shorter than   16522 bp
  75% of sequence in fragments shorter than   18388 bp
  80% of sequence in fragments shorter than   20677 bp
  85% of sequence in fragments shorter than   23737 bp
  90% of sequence in fragments shorter than   28203 bp
  95% of sequence in fragments shorter than   37129 bp

# PacI.SexAI (TTAATTAA+ACCWGGT) double digest, 457 thousand fragments (159455+297900 sites), 6520 bp average fragment length
   5% of sequence in fragments shorter than    2335 bp
  10% of sequence in fragments shorter than    3531 bp
  15% of sequence in fragments shorter than    4568 bp
  20% of sequence in fragments shorter than    5549 bp
  25% of sequence in fragments shorter than    6490 bp
  30% of sequence in fragments shorter than    7434 bp
  35% of sequence in fragments shorter than    8382 bp
  40% of sequence in fragments shorter than    9361 bp
  45% of sequence in fragments shorter than   10386 bp
  50% of sequence in fragments shorter than   11484 bp
  55% of sequence in fragments shorter than   12660 bp
  60% of sequence in fragments shorter than   13937 bp
  65% of sequence in fragments shorter than   15350 bp
  70% of sequence in fragments shorter than   16960 bp
  75% of sequence in fragments shorter than   18831 bp
  80% of sequence in fragments shorter than   21095 bp
  85% of sequence in fragments shorter than   24116 bp
  90% of sequence in fragments shorter than   28431 bp
  95% of sequence in fragments shorter than   37488 bp

# BstEII.XhoI (GGTNACC+CTCGAG) double digest, 456 thousand fragments (335865+121308 sites), 6522 bp average fragment length
   5% of sequence in fragments shorter than    2263 bp
  10% of sequence in fragments shorter than    3487 bp
  15% of sequence in fragments shorter than    4575 bp
  20% of sequence in fragments shorter than    5573 bp
  25% of sequence in fragments shorter than    6572 bp
  30% of sequence in fragments shorter than    7563 bp
  35% of sequence in fragments shorter than    8585 bp
  40% of sequence in fragments shorter than    9646 bp
  45% of sequence in fragments shorter than   10781 bp
  50% of sequence in fragments shorter than   11985 bp
  55% of sequence in fragments shorter than   13312 bp
  60% of sequence in fragments shorter than   14761 bp
  65% of sequence in fragments shorter than   16368 bp
  70% of sequence in fragments shorter than   18246 bp
  75% of sequence in fragments shorter than   20469 bp
  80% of sequence in fragments shorter than   23155 bp
  85% of sequence in fragments shorter than   26831 bp
  90% of sequence in fragments shorter than   32308 bp
  95% of sequence in fragments shorter than   43765 bp

# KasI.SwaI (GGCGCC+ATTTAAAT) double digest, 456 thousand fragments (230381+226602 sites), 6525 bp average fragment length
   5% of sequence in fragments shorter than    2289 bp
  10% of sequence in fragments shorter than    3558 bp
  15% of sequence in fragments shorter than    4662 bp
  20% of sequence in fragments shorter than    5702 bp
  25% of sequence in fragments shorter than    6715 bp
  30% of sequence in fragments shorter than    7746 bp
  35% of sequence in fragments shorter than    8815 bp
  40% of sequence in fragments shorter than    9901 bp
  45% of sequence in fragments shorter than   11050 bp
  50% of sequence in fragments shorter than   12286 bp
  55% of sequence in fragments shorter than   13638 bp
  60% of sequence in fragments shorter than   15107 bp
  65% of sequence in fragments shorter than   16765 bp
  70% of sequence in fragments shorter than   18700 bp
  75% of sequence in fragments shorter than   20950 bp
  80% of sequence in fragments shorter than   23740 bp
  85% of sequence in fragments shorter than   27417 bp
  90% of sequence in fragments shorter than   33048 bp
  95% of sequence in fragments shorter than   44869 bp

# HpaI.SacII (GTTAAC+CCGCGG) double digest, 456 thousand fragments (389418+66901 sites), 6534 bp average fragment length
   5% of sequence in fragments shorter than    2362 bp
  10% of sequence in fragments shorter than    3607 bp
  15% of sequence in fragments shorter than    4670 bp
  20% of sequence in fragments shorter than    5678 bp
  25% of sequence in fragments shorter than    6662 bp
  30% of sequence in fragments shorter than    7649 bp
  35% of sequence in fragments shorter than    8648 bp
  40% of sequence in fragments shorter than    9676 bp
  45% of sequence in fragments shorter than   10757 bp
  50% of sequence in fragments shorter than   11901 bp
  55% of sequence in fragments shorter than   13127 bp
  60% of sequence in fragments shorter than   14480 bp
  65% of sequence in fragments shorter than   15974 bp
  70% of sequence in fragments shorter than   17700 bp
  75% of sequence in fragments shorter than   19638 bp
  80% of sequence in fragments shorter than   22069 bp
  85% of sequence in fragments shorter than   25153 bp
  90% of sequence in fragments shorter than   29712 bp
  95% of sequence in fragments shorter than   39155 bp

# EcoRV.NotI (GATATC+GCGGCCGC) double digest, 455 thousand fragments (446473+9795 sites), 6537 bp average fragment length
   5% of sequence in fragments shorter than    2236 bp
  10% of sequence in fragments shorter than    3459 bp
  15% of sequence in fragments shorter than    4514 bp
  20% of sequence in fragments shorter than    5502 bp
  25% of sequence in fragments shorter than    6473 bp
  30% of sequence in fragments shorter than    7454 bp
  35% of sequence in fragments shorter than    8452 bp
  40% of sequence in fragments shorter than    9494 bp
  45% of sequence in fragments shorter than   10598 bp
  50% of sequence in fragments shorter than   11777 bp
  55% of sequence in fragments shorter than   13055 bp
  60% of sequence in fragments shorter than   14460 bp
  65% of sequence in fragments shorter than   16034 bp
  70% of sequence in fragments shorter than   17833 bp
  75% of sequence in fragments shorter than   19979 bp
  80% of sequence in fragments shorter than   22642 bp
  85% of sequence in fragments shorter than   26221 bp
  90% of sequence in fragments shorter than   31482 bp
  95% of sequence in fragments shorter than   42044 bp

# EcoRV.RsrII (GATATC+CGGWCCG) double digest, 455 thousand fragments (446473+9764 sites), 6537 bp average fragment length
   5% of sequence in fragments shorter than    2234 bp
  10% of sequence in fragments shorter than    3450 bp
  15% of sequence in fragments shorter than    4506 bp
  20% of sequence in fragments shorter than    5490 bp
  25% of sequence in fragments shorter than    6459 bp
  30% of sequence in fragments shorter than    7442 bp
  35% of sequence in fragments shorter than    8446 bp
  40% of sequence in fragments shorter than    9485 bp
  45% of sequence in fragments shorter than   10586 bp
  50% of sequence in fragments shorter than   11759 bp
  55% of sequence in fragments shorter than   13037 bp
  60% of sequence in fragments shorter than   14442 bp
  65% of sequence in fragments shorter than   16011 bp
  70% of sequence in fragments shorter than   17816 bp
  75% of sequence in fragments shorter than   19957 bp
  80% of sequence in fragments shorter than   22627 bp
  85% of sequence in fragments shorter than   26184 bp
  90% of sequence in fragments shorter than   31487 bp
  95% of sequence in fragments shorter than   42048 bp

# Hpy99I.SexAI (CGWCG+ACCWGGT) double digest, 455 thousand fragments (157917+297900 sites), 6543 bp average fragment length
   5% of sequence in fragments shorter than    2274 bp
  10% of sequence in fragments shorter than    3574 bp
  15% of sequence in fragments shorter than    4728 bp
  20% of sequence in fragments shorter than    5840 bp
  25% of sequence in fragments shorter than    6930 bp
  30% of sequence in fragments shorter than    8028 bp
  35% of sequence in fragments shorter than    9153 bp
  40% of sequence in fragments shorter than   10335 bp
  45% of sequence in fragments shorter than   11578 bp
  50% of sequence in fragments shorter than   12902 bp
  55% of sequence in fragments shorter than   14346 bp
  60% of sequence in fragments shorter than   15922 bp
  65% of sequence in fragments shorter than   17704 bp
  70% of sequence in fragments shorter than   19753 bp
  75% of sequence in fragments shorter than   22204 bp
  80% of sequence in fragments shorter than   25108 bp
  85% of sequence in fragments shorter than   29021 bp
  90% of sequence in fragments shorter than   34836 bp
  95% of sequence in fragments shorter than   46258 bp

# AgeI.SpeI (ACCGGT+ACTAGT) double digest, 453 thousand fragments (53386+400286 sites), 6573 bp average fragment length
   5% of sequence in fragments shorter than    2284 bp
  10% of sequence in fragments shorter than    3449 bp
  15% of sequence in fragments shorter than    4405 bp
  20% of sequence in fragments shorter than    5366 bp
  25% of sequence in fragments shorter than    6300 bp
  30% of sequence in fragments shorter than    7223 bp
  35% of sequence in fragments shorter than    8178 bp
  40% of sequence in fragments shorter than    9164 bp
  45% of sequence in fragments shorter than   10198 bp
  50% of sequence in fragments shorter than   11316 bp
  55% of sequence in fragments shorter than   12491 bp
  60% of sequence in fragments shorter than   13805 bp
  65% of sequence in fragments shorter than   15304 bp
  70% of sequence in fragments shorter than   16971 bp
  75% of sequence in fragments shorter than   18957 bp
  80% of sequence in fragments shorter than   21432 bp
  85% of sequence in fragments shorter than   24732 bp
  90% of sequence in fragments shorter than   29714 bp
  95% of sequence in fragments shorter than   39746 bp

# BamHI.ClaI (GGATCC+ATCGAT) double digest, 452 thousand fragments (365999+86477 sites), 6590 bp average fragment length
   5% of sequence in fragments shorter than    2261 bp
  10% of sequence in fragments shorter than    3461 bp
  15% of sequence in fragments shorter than    4504 bp
  20% of sequence in fragments shorter than    5483 bp
  25% of sequence in fragments shorter than    6434 bp
  30% of sequence in fragments shorter than    7394 bp
  35% of sequence in fragments shorter than    8363 bp
  40% of sequence in fragments shorter than    9380 bp
  45% of sequence in fragments shorter than   10442 bp
  50% of sequence in fragments shorter than   11571 bp
  55% of sequence in fragments shorter than   12783 bp
  60% of sequence in fragments shorter than   14124 bp
  65% of sequence in fragments shorter than   15617 bp
  70% of sequence in fragments shorter than   17311 bp
  75% of sequence in fragments shorter than   19302 bp
  80% of sequence in fragments shorter than   21787 bp
  85% of sequence in fragments shorter than   24998 bp
  90% of sequence in fragments shorter than   29701 bp
  95% of sequence in fragments shorter than   38748 bp

# AscI.EcoRV (GGCGCGCC+GATATC) double digest, 450 thousand fragments (4751+446473 sites), 6610 bp average fragment length
   5% of sequence in fragments shorter than    2249 bp
  10% of sequence in fragments shorter than    3486 bp
  15% of sequence in fragments shorter than    4552 bp
  20% of sequence in fragments shorter than    5544 bp
  25% of sequence in fragments shorter than    6534 bp
  30% of sequence in fragments shorter than    7527 bp
  35% of sequence in fragments shorter than    8539 bp
  40% of sequence in fragments shorter than    9594 bp
  45% of sequence in fragments shorter than   10726 bp
  50% of sequence in fragments shorter than   11914 bp
  55% of sequence in fragments shorter than   13220 bp
  60% of sequence in fragments shorter than   14661 bp
  65% of sequence in fragments shorter than   16260 bp
  70% of sequence in fragments shorter than   18096 bp
  75% of sequence in fragments shorter than   20329 bp
  80% of sequence in fragments shorter than   23135 bp
  85% of sequence in fragments shorter than   26858 bp
  90% of sequence in fragments shorter than   32516 bp
  95% of sequence in fragments shorter than   44060 bp

# HpaI.PspXI (GTTAAC+VCTCGAGB) double digest, 448 thousand fragments (389418+59193 sites), 6647 bp average fragment length
   5% of sequence in fragments shorter than    2338 bp
  10% of sequence in fragments shorter than    3527 bp
  15% of sequence in fragments shorter than    4541 bp
  20% of sequence in fragments shorter than    5501 bp
  25% of sequence in fragments shorter than    6438 bp
  30% of sequence in fragments shorter than    7365 bp
  35% of sequence in fragments shorter than    8325 bp
  40% of sequence in fragments shorter than    9306 bp
  45% of sequence in fragments shorter than   10313 bp
  50% of sequence in fragments shorter than   11412 bp
  55% of sequence in fragments shorter than   12585 bp
  60% of sequence in fragments shorter than   13864 bp
  65% of sequence in fragments shorter than   15296 bp
  70% of sequence in fragments shorter than   16915 bp
  75% of sequence in fragments shorter than   18807 bp
  80% of sequence in fragments shorter than   21107 bp
  85% of sequence in fragments shorter than   24097 bp
  90% of sequence in fragments shorter than   28464 bp
  95% of sequence in fragments shorter than   37660 bp

# AatII.SapI (GACGTC+GCTCTTC) double digest, 447 thousand fragments (71089+377161 sites), 6653 bp average fragment length
   5% of sequence in fragments shorter than    2295 bp
  10% of sequence in fragments shorter than    3534 bp
  15% of sequence in fragments shorter than    4607 bp
  20% of sequence in fragments shorter than    5603 bp
  25% of sequence in fragments shorter than    6580 bp
  30% of sequence in fragments shorter than    7573 bp
  35% of sequence in fragments shorter than    8575 bp
  40% of sequence in fragments shorter than    9618 bp
  45% of sequence in fragments shorter than   10716 bp
  50% of sequence in fragments shorter than   11916 bp
  55% of sequence in fragments shorter than   13184 bp
  60% of sequence in fragments shorter than   14554 bp
  65% of sequence in fragments shorter than   16108 bp
  70% of sequence in fragments shorter than   17869 bp
  75% of sequence in fragments shorter than   19946 bp
  80% of sequence in fragments shorter than   22507 bp
  85% of sequence in fragments shorter than   25871 bp
  90% of sequence in fragments shorter than   30881 bp
  95% of sequence in fragments shorter than   40844 bp

# AatII.SmaI (GACGTC+CCCGGG) double digest, 447 thousand fragments (71089+376940 sites), 6656 bp average fragment length
   5% of sequence in fragments shorter than    2092 bp
  10% of sequence in fragments shorter than    3462 bp
  15% of sequence in fragments shorter than    4774 bp
  20% of sequence in fragments shorter than    6108 bp
  25% of sequence in fragments shorter than    7513 bp
  30% of sequence in fragments shorter than    8985 bp
  35% of sequence in fragments shorter than   10570 bp
  40% of sequence in fragments shorter than   12268 bp
  45% of sequence in fragments shorter than   14154 bp
  50% of sequence in fragments shorter than   16272 bp
  55% of sequence in fragments shorter than   18604 bp
  60% of sequence in fragments shorter than   21300 bp
  65% of sequence in fragments shorter than   24336 bp
  70% of sequence in fragments shorter than   27973 bp
  75% of sequence in fragments shorter than   32363 bp
  80% of sequence in fragments shorter than   37935 bp
  85% of sequence in fragments shorter than   45317 bp
  90% of sequence in fragments shorter than   56254 bp
  95% of sequence in fragments shorter than   78026 bp

# KpnI.PacI (GGTACC+TTAATTAA) double digest, 447 thousand fragments (287974+159455 sites), 6664 bp average fragment length
   5% of sequence in fragments shorter than    2262 bp
  10% of sequence in fragments shorter than    3506 bp
  15% of sequence in fragments shorter than    4586 bp
  20% of sequence in fragments shorter than    5605 bp
  25% of sequence in fragments shorter than    6587 bp
  30% of sequence in fragments shorter than    7578 bp
  35% of sequence in fragments shorter than    8579 bp
  40% of sequence in fragments shorter than    9615 bp
  45% of sequence in fragments shorter than   10699 bp
  50% of sequence in fragments shorter than   11855 bp
  55% of sequence in fragments shorter than   13074 bp
  60% of sequence in fragments shorter than   14399 bp
  65% of sequence in fragments shorter than   15857 bp
  70% of sequence in fragments shorter than   17544 bp
  75% of sequence in fragments shorter than   19547 bp
  80% of sequence in fragments shorter than   21990 bp
  85% of sequence in fragments shorter than   25154 bp
  90% of sequence in fragments shorter than   29869 bp
  95% of sequence in fragments shorter than   39705 bp

# NheI.PacI (GCTAGC+TTAATTAA) double digest, 446 thousand fragments (287384+159455 sites), 6673 bp average fragment length
   5% of sequence in fragments shorter than    2282 bp
  10% of sequence in fragments shorter than    3466 bp
  15% of sequence in fragments shorter than    4606 bp
  20% of sequence in fragments shorter than    5656 bp
  25% of sequence in fragments shorter than    6684 bp
  30% of sequence in fragments shorter than    7703 bp
  35% of sequence in fragments shorter than    8745 bp
  40% of sequence in fragments shorter than    9819 bp
  45% of sequence in fragments shorter than   10936 bp
  50% of sequence in fragments shorter than   12105 bp
  55% of sequence in fragments shorter than   13405 bp
  60% of sequence in fragments shorter than   14789 bp
  65% of sequence in fragments shorter than   16361 bp
  70% of sequence in fragments shorter than   18174 bp
  75% of sequence in fragments shorter than   20291 bp
  80% of sequence in fragments shorter than   22869 bp
  85% of sequence in fragments shorter than   26231 bp
  90% of sequence in fragments shorter than   31331 bp
  95% of sequence in fragments shorter than   41729 bp

# Hpy99I.KpnI (CGWCG+GGTACC) double digest, 445 thousand fragments (157917+287974 sites), 6688 bp average fragment length
   5% of sequence in fragments shorter than    2233 bp
  10% of sequence in fragments shorter than    3610 bp
  15% of sequence in fragments shorter than    4844 bp
  20% of sequence in fragments shorter than    6009 bp
  25% of sequence in fragments shorter than    7145 bp
  30% of sequence in fragments shorter than    8286 bp
  35% of sequence in fragments shorter than    9459 bp
  40% of sequence in fragments shorter than   10670 bp
  45% of sequence in fragments shorter than   11943 bp
  50% of sequence in fragments shorter than   13294 bp
  55% of sequence in fragments shorter than   14762 bp
  60% of sequence in fragments shorter than   16341 bp
  65% of sequence in fragments shorter than   18113 bp
  70% of sequence in fragments shorter than   20112 bp
  75% of sequence in fragments shorter than   22459 bp
  80% of sequence in fragments shorter than   25366 bp
  85% of sequence in fragments shorter than   29073 bp
  90% of sequence in fragments shorter than   34734 bp
  95% of sequence in fragments shorter than   45660 bp

# Hpy99I.NheI (CGWCG+GCTAGC) double digest, 444 thousand fragments (157917+287384 sites), 6698 bp average fragment length
   5% of sequence in fragments shorter than    2275 bp
  10% of sequence in fragments shorter than    3617 bp
  15% of sequence in fragments shorter than    4891 bp
  20% of sequence in fragments shorter than    6068 bp
  25% of sequence in fragments shorter than    7224 bp
  30% of sequence in fragments shorter than    8383 bp
  35% of sequence in fragments shorter than    9555 bp
  40% of sequence in fragments shorter than   10756 bp
  45% of sequence in fragments shorter than   11990 bp
  50% of sequence in fragments shorter than   13345 bp
  55% of sequence in fragments shorter than   14754 bp
  60% of sequence in fragments shorter than   16332 bp
  65% of sequence in fragments shorter than   18075 bp
  70% of sequence in fragments shorter than   20042 bp
  75% of sequence in fragments shorter than   22399 bp
  80% of sequence in fragments shorter than   25204 bp
  85% of sequence in fragments shorter than   28783 bp
  90% of sequence in fragments shorter than   34019 bp
  95% of sequence in fragments shorter than   44255 bp

# SacII.SapI (CCGCGG+GCTCTTC) double digest, 443 thousand fragments (66901+377161 sites), 6715 bp average fragment length
   5% of sequence in fragments shorter than    2383 bp
  10% of sequence in fragments shorter than    3737 bp
  15% of sequence in fragments shorter than    4897 bp
  20% of sequence in fragments shorter than    5977 bp
  25% of sequence in fragments shorter than    7052 bp
  30% of sequence in fragments shorter than    8117 bp
  35% of sequence in fragments shorter than    9207 bp
  40% of sequence in fragments shorter than   10344 bp
  45% of sequence in fragments shorter than   11561 bp
  50% of sequence in fragments shorter than   12837 bp
  55% of sequence in fragments shorter than   14204 bp
  60% of sequence in fragments shorter than   15713 bp
  65% of sequence in fragments shorter than   17384 bp
  70% of sequence in fragments shorter than   19313 bp
  75% of sequence in fragments shorter than   21583 bp
  80% of sequence in fragments shorter than   24413 bp
  85% of sequence in fragments shorter than   28147 bp
  90% of sequence in fragments shorter than   33808 bp
  95% of sequence in fragments shorter than   45284 bp

# SacII.SmaI (CCGCGG+CCCGGG) double digest, 443 thousand fragments (66901+376940 sites), 6718 bp average fragment length
   5% of sequence in fragments shorter than    2218 bp
  10% of sequence in fragments shorter than    3767 bp
  15% of sequence in fragments shorter than    5275 bp
  20% of sequence in fragments shorter than    6839 bp
  25% of sequence in fragments shorter than    8494 bp
  30% of sequence in fragments shorter than   10250 bp
  35% of sequence in fragments shorter than   12190 bp
  40% of sequence in fragments shorter than   14309 bp
  45% of sequence in fragments shorter than   16649 bp
  50% of sequence in fragments shorter than   19297 bp
  55% of sequence in fragments shorter than   22232 bp
  60% of sequence in fragments shorter than   25616 bp
  65% of sequence in fragments shorter than   29569 bp
  70% of sequence in fragments shorter than   34224 bp
  75% of sequence in fragments shorter than   39752 bp
  80% of sequence in fragments shorter than   46747 bp
  85% of sequence in fragments shorter than   55853 bp
  90% of sequence in fragments shorter than   70806 bp
  95% of sequence in fragments shorter than  104056 bp

# AgeI.HpaI (ACCGGT+GTTAAC) double digest, 442 thousand fragments (53386+389418 sites), 6734 bp average fragment length
   5% of sequence in fragments shorter than    2374 bp
  10% of sequence in fragments shorter than    3579 bp
  15% of sequence in fragments shorter than    4608 bp
  20% of sequence in fragments shorter than    5578 bp
  25% of sequence in fragments shorter than    6535 bp
  30% of sequence in fragments shorter than    7488 bp
  35% of sequence in fragments shorter than    8463 bp
  40% of sequence in fragments shorter than    9460 bp
  45% of sequence in fragments shorter than   10496 bp
  50% of sequence in fragments shorter than   11630 bp
  55% of sequence in fragments shorter than   12825 bp
  60% of sequence in fragments shorter than   14132 bp
  65% of sequence in fragments shorter than   15588 bp
  70% of sequence in fragments shorter than   17273 bp
  75% of sequence in fragments shorter than   19230 bp
  80% of sequence in fragments shorter than   21648 bp
  85% of sequence in fragments shorter than   24862 bp
  90% of sequence in fragments shorter than   29530 bp
  95% of sequence in fragments shorter than   39516 bp

# PmeI.SpeI (GTTTAAAC+ACTAGT) double digest, 441 thousand fragments (42094+400286 sites), 6742 bp average fragment length
   5% of sequence in fragments shorter than    2306 bp
  10% of sequence in fragments shorter than    3505 bp
  15% of sequence in fragments shorter than    4551 bp
  20% of sequence in fragments shorter than    5540 bp
  25% of sequence in fragments shorter than    6495 bp
  30% of sequence in fragments shorter than    7452 bp
  35% of sequence in fragments shorter than    8446 bp
  40% of sequence in fragments shorter than    9478 bp
  45% of sequence in fragments shorter than   10575 bp
  50% of sequence in fragments shorter than   11744 bp
  55% of sequence in fragments shorter than   13006 bp
  60% of sequence in fragments shorter than   14440 bp
  65% of sequence in fragments shorter than   16071 bp
  70% of sequence in fragments shorter than   17923 bp
  75% of sequence in fragments shorter than   20188 bp
  80% of sequence in fragments shorter than   23016 bp
  85% of sequence in fragments shorter than   26972 bp
  90% of sequence in fragments shorter than   33128 bp
  95% of sequence in fragments shorter than   47568 bp

# AatII.BamHI (GACGTC+GGATCC) double digest, 436 thousand fragments (71089+365999 sites), 6823 bp average fragment length
   5% of sequence in fragments shorter than    2329 bp
  10% of sequence in fragments shorter than    3588 bp
  15% of sequence in fragments shorter than    4692 bp
  20% of sequence in fragments shorter than    5722 bp
  25% of sequence in fragments shorter than    6732 bp
  30% of sequence in fragments shorter than    7757 bp
  35% of sequence in fragments shorter than    8803 bp
  40% of sequence in fragments shorter than    9907 bp
  45% of sequence in fragments shorter than   11062 bp
  50% of sequence in fragments shorter than   12293 bp
  55% of sequence in fragments shorter than   13615 bp
  60% of sequence in fragments shorter than   15098 bp
  65% of sequence in fragments shorter than   16746 bp
  70% of sequence in fragments shorter than   18633 bp
  75% of sequence in fragments shorter than   20872 bp
  80% of sequence in fragments shorter than   23666 bp
  85% of sequence in fragments shorter than   27313 bp
  90% of sequence in fragments shorter than   32417 bp
  95% of sequence in fragments shorter than   42528 bp

# PspXI.SapI (VCTCGAGB+GCTCTTC) double digest, 436 thousand fragments (59193+377161 sites), 6834 bp average fragment length
   5% of sequence in fragments shorter than    2349 bp
  10% of sequence in fragments shorter than    3626 bp
  15% of sequence in fragments shorter than    4721 bp
  20% of sequence in fragments shorter than    5740 bp
  25% of sequence in fragments shorter than    6747 bp
  30% of sequence in fragments shorter than    7766 bp
  35% of sequence in fragments shorter than    8792 bp
  40% of sequence in fragments shorter than    9865 bp
  45% of sequence in fragments shorter than   11006 bp
  50% of sequence in fragments shorter than   12205 bp
  55% of sequence in fragments shorter than   13509 bp
  60% of sequence in fragments shorter than   14946 bp
  65% of sequence in fragments shorter than   16524 bp
  70% of sequence in fragments shorter than   18326 bp
  75% of sequence in fragments shorter than   20465 bp
  80% of sequence in fragments shorter than   23095 bp
  85% of sequence in fragments shorter than   26559 bp
  90% of sequence in fragments shorter than   31806 bp
  95% of sequence in fragments shorter than   42642 bp

# PspXI.SmaI (VCTCGAGB+CCCGGG) double digest, 435 thousand fragments (59193+376940 sites), 6837 bp average fragment length
   5% of sequence in fragments shorter than    2138 bp
  10% of sequence in fragments shorter than    3537 bp
  15% of sequence in fragments shorter than    4889 bp
  20% of sequence in fragments shorter than    6275 bp
  25% of sequence in fragments shorter than    7728 bp
  30% of sequence in fragments shorter than    9270 bp
  35% of sequence in fragments shorter than   10931 bp
  40% of sequence in fragments shorter than   12754 bp
  45% of sequence in fragments shorter than   14758 bp
  50% of sequence in fragments shorter than   16987 bp
  55% of sequence in fragments shorter than   19521 bp
  60% of sequence in fragments shorter than   22367 bp
  65% of sequence in fragments shorter than   25686 bp
  70% of sequence in fragments shorter than   29658 bp
  75% of sequence in fragments shorter than   34390 bp
  80% of sequence in fragments shorter than   40394 bp
  85% of sequence in fragments shorter than   48041 bp
  90% of sequence in fragments shorter than   60393 bp
  95% of sequence in fragments shorter than   86747 bp

# BamHI.SacII (GGATCC+CCGCGG) double digest, 432 thousand fragments (365999+66901 sites), 6888 bp average fragment length
   5% of sequence in fragments shorter than    2424 bp
  10% of sequence in fragments shorter than    3817 bp
  15% of sequence in fragments shorter than    5029 bp
  20% of sequence in fragments shorter than    6169 bp
  25% of sequence in fragments shorter than    7288 bp
  30% of sequence in fragments shorter than    8422 bp
  35% of sequence in fragments shorter than    9598 bp
  40% of sequence in fragments shorter than   10801 bp
  45% of sequence in fragments shorter than   12082 bp
  50% of sequence in fragments shorter than   13454 bp
  55% of sequence in fragments shorter than   14953 bp
  60% of sequence in fragments shorter than   16594 bp
  65% of sequence in fragments shorter than   18430 bp
  70% of sequence in fragments shorter than   20561 bp
  75% of sequence in fragments shorter than   23122 bp
  80% of sequence in fragments shorter than   26252 bp
  85% of sequence in fragments shorter than   30311 bp
  90% of sequence in fragments shorter than   36179 bp
  95% of sequence in fragments shorter than   47551 bp

# SalI.SpeI (GTCGAC+ACTAGT) double digest, 431 thousand fragments (31396+400286 sites), 6909 bp average fragment length
   5% of sequence in fragments shorter than    2380 bp
  10% of sequence in fragments shorter than    3613 bp
  15% of sequence in fragments shorter than    4687 bp
  20% of sequence in fragments shorter than    5686 bp
  25% of sequence in fragments shorter than    6654 bp
  30% of sequence in fragments shorter than    7622 bp
  35% of sequence in fragments shorter than    8623 bp
  40% of sequence in fragments shorter than    9667 bp
  45% of sequence in fragments shorter than   10775 bp
  50% of sequence in fragments shorter than   11936 bp
  55% of sequence in fragments shorter than   13201 bp
  60% of sequence in fragments shorter than   14605 bp
  65% of sequence in fragments shorter than   16195 bp
  70% of sequence in fragments shorter than   18023 bp
  75% of sequence in fragments shorter than   20216 bp
  80% of sequence in fragments shorter than   22903 bp
  85% of sequence in fragments shorter than   26516 bp
  90% of sequence in fragments shorter than   32112 bp
  95% of sequence in fragments shorter than   44037 bp

# HpaI.PmeI (GTTAAC+GTTTAAAC) double digest, 431 thousand fragments (389418+42094 sites), 6911 bp average fragment length
   5% of sequence in fragments shorter than    2412 bp
  10% of sequence in fragments shorter than    3641 bp
  15% of sequence in fragments shorter than    4699 bp
  20% of sequence in fragments shorter than    5704 bp
  25% of sequence in fragments shorter than    6697 bp
  30% of sequence in fragments shorter than    7690 bp
  35% of sequence in fragments shorter than    8711 bp
  40% of sequence in fragments shorter than    9757 bp
  45% of sequence in fragments shorter than   10867 bp
  50% of sequence in fragments shorter than   12055 bp
  55% of sequence in fragments shorter than   13334 bp
  60% of sequence in fragments shorter than   14757 bp
  65% of sequence in fragments shorter than   16345 bp
  70% of sequence in fragments shorter than   18202 bp
  75% of sequence in fragments shorter than   20310 bp
  80% of sequence in fragments shorter than   23017 bp
  85% of sequence in fragments shorter than   26621 bp
  90% of sequence in fragments shorter than   32180 bp
  95% of sequence in fragments shorter than   44297 bp

# AgeI.SapI (ACCGGT+GCTCTTC) double digest, 430 thousand fragments (53386+377161 sites), 6926 bp average fragment length
   5% of sequence in fragments shorter than    2409 bp
  10% of sequence in fragments shorter than    3707 bp
  15% of sequence in fragments shorter than    4821 bp
  20% of sequence in fragments shorter than    5852 bp
  25% of sequence in fragments shorter than    6878 bp
  30% of sequence in fragments shorter than    7896 bp
  35% of sequence in fragments shorter than    8944 bp
  40% of sequence in fragments shorter than   10027 bp
  45% of sequence in fragments shorter than   11168 bp
  50% of sequence in fragments shorter than   12372 bp
  55% of sequence in fragments shorter than   13673 bp
  60% of sequence in fragments shorter than   15112 bp
  65% of sequence in fragments shorter than   16701 bp
  70% of sequence in fragments shorter than   18492 bp
  75% of sequence in fragments shorter than   20666 bp
  80% of sequence in fragments shorter than   23274 bp
  85% of sequence in fragments shorter than   26778 bp
  90% of sequence in fragments shorter than   31882 bp
  95% of sequence in fragments shorter than   42662 bp

# AgeI.SmaI (ACCGGT+CCCGGG) double digest, 430 thousand fragments (53386+376940 sites), 6929 bp average fragment length
   5% of sequence in fragments shorter than    2189 bp
  10% of sequence in fragments shorter than    3610 bp
  15% of sequence in fragments shorter than    4966 bp
  20% of sequence in fragments shorter than    6346 bp
  25% of sequence in fragments shorter than    7766 bp
  30% of sequence in fragments shorter than    9265 bp
  35% of sequence in fragments shorter than   10881 bp
  40% of sequence in fragments shorter than   12626 bp
  45% of sequence in fragments shorter than   14523 bp
  50% of sequence in fragments shorter than   16613 bp
  55% of sequence in fragments shorter than   18998 bp
  60% of sequence in fragments shorter than   21690 bp
  65% of sequence in fragments shorter than   24726 bp
  70% of sequence in fragments shorter than   28331 bp
  75% of sequence in fragments shorter than   32656 bp
  80% of sequence in fragments shorter than   38194 bp
  85% of sequence in fragments shorter than   45551 bp
  90% of sequence in fragments shorter than   56644 bp
  95% of sequence in fragments shorter than   81491 bp

# SexAI.SnaBI (ACCWGGT+TACGTA) double digest, 429 thousand fragments (297900+132299 sites), 6932 bp average fragment length
   5% of sequence in fragments shorter than    2432 bp
  10% of sequence in fragments shorter than    3679 bp
  15% of sequence in fragments shorter than    4781 bp
  20% of sequence in fragments shorter than    5820 bp
  25% of sequence in fragments shorter than    6823 bp
  30% of sequence in fragments shorter than    7808 bp
  35% of sequence in fragments shorter than    8817 bp
  40% of sequence in fragments shorter than    9866 bp
  45% of sequence in fragments shorter than   10961 bp
  50% of sequence in fragments shorter than   12128 bp
  55% of sequence in fragments shorter than   13348 bp
  60% of sequence in fragments shorter than   14702 bp
  65% of sequence in fragments shorter than   16183 bp
  70% of sequence in fragments shorter than   17859 bp
  75% of sequence in fragments shorter than   19810 bp
  80% of sequence in fragments shorter than   22219 bp
  85% of sequence in fragments shorter than   25327 bp
  90% of sequence in fragments shorter than   29868 bp
  95% of sequence in fragments shorter than   39262 bp

# BamHI.PspXI (GGATCC+VCTCGAGB) double digest, 424 thousand fragments (365999+59193 sites), 7014 bp average fragment length
   5% of sequence in fragments shorter than    2395 bp
  10% of sequence in fragments shorter than    3682 bp
  15% of sequence in fragments shorter than    4813 bp
  20% of sequence in fragments shorter than    5869 bp
  25% of sequence in fragments shorter than    6911 bp
  30% of sequence in fragments shorter than    7974 bp
  35% of sequence in fragments shorter than    9065 bp
  40% of sequence in fragments shorter than   10206 bp
  45% of sequence in fragments shorter than   11399 bp
  50% of sequence in fragments shorter than   12676 bp
  55% of sequence in fragments shorter than   14066 bp
  60% of sequence in fragments shorter than   15580 bp
  65% of sequence in fragments shorter than   17297 bp
  70% of sequence in fragments shorter than   19240 bp
  75% of sequence in fragments shorter than   21569 bp
  80% of sequence in fragments shorter than   24426 bp
  85% of sequence in fragments shorter than   28181 bp
  90% of sequence in fragments shorter than   33715 bp
  95% of sequence in fragments shorter than   44527 bp

# NaeI.SexAI (GCCGGC+ACCWGGT) double digest, 423 thousand fragments (125692+297900 sites), 7039 bp average fragment length
   5% of sequence in fragments shorter than    2410 bp
  10% of sequence in fragments shorter than    3808 bp
  15% of sequence in fragments shorter than    5041 bp
  20% of sequence in fragments shorter than    6252 bp
  25% of sequence in fragments shorter than    7426 bp
  30% of sequence in fragments shorter than    8614 bp
  35% of sequence in fragments shorter than    9842 bp
  40% of sequence in fragments shorter than   11146 bp
  45% of sequence in fragments shorter than   12526 bp
  50% of sequence in fragments shorter than   13968 bp
  55% of sequence in fragments shorter than   15560 bp
  60% of sequence in fragments shorter than   17289 bp
  65% of sequence in fragments shorter than   19247 bp
  70% of sequence in fragments shorter than   21454 bp
  75% of sequence in fragments shorter than   24078 bp
  80% of sequence in fragments shorter than   27335 bp
  85% of sequence in fragments shorter than   31763 bp
  90% of sequence in fragments shorter than   38259 bp
  95% of sequence in fragments shorter than   52101 bp

# BstEII.ClaI (GGTNACC+ATCGAT) double digest, 422 thousand fragments (335865+86477 sites), 7061 bp average fragment length
   5% of sequence in fragments shorter than    2444 bp
  10% of sequence in fragments shorter than    3739 bp
  15% of sequence in fragments shorter than    4864 bp
  20% of sequence in fragments shorter than    5910 bp
  25% of sequence in fragments shorter than    6932 bp
  30% of sequence in fragments shorter than    7958 bp
  35% of sequence in fragments shorter than    8999 bp
  40% of sequence in fragments shorter than   10082 bp
  45% of sequence in fragments shorter than   11217 bp
  50% of sequence in fragments shorter than   12428 bp
  55% of sequence in fragments shorter than   13739 bp
  60% of sequence in fragments shorter than   15171 bp
  65% of sequence in fragments shorter than   16778 bp
  70% of sequence in fragments shorter than   18618 bp
  75% of sequence in fragments shorter than   20770 bp
  80% of sequence in fragments shorter than   23445 bp
  85% of sequence in fragments shorter than   26926 bp
  90% of sequence in fragments shorter than   32092 bp
  95% of sequence in fragments shorter than   42738 bp

# MluI.SpeI (ACGCGT+ACTAGT) double digest, 421 thousand fragments (21415+400286 sites), 7072 bp average fragment length
   5% of sequence in fragments shorter than    2437 bp
  10% of sequence in fragments shorter than    3703 bp
  15% of sequence in fragments shorter than    4800 bp
  20% of sequence in fragments shorter than    5823 bp
  25% of sequence in fragments shorter than    6821 bp
  30% of sequence in fragments shorter than    7814 bp
  35% of sequence in fragments shorter than    8846 bp
  40% of sequence in fragments shorter than    9917 bp
  45% of sequence in fragments shorter than   11043 bp
  50% of sequence in fragments shorter than   12229 bp
  55% of sequence in fragments shorter than   13527 bp
  60% of sequence in fragments shorter than   14985 bp
  65% of sequence in fragments shorter than   16594 bp
  70% of sequence in fragments shorter than   18448 bp
  75% of sequence in fragments shorter than   20623 bp
  80% of sequence in fragments shorter than   23349 bp
  85% of sequence in fragments shorter than   27005 bp
  90% of sequence in fragments shorter than   32637 bp
  95% of sequence in fragments shorter than   44306 bp

# HpaI.SalI (GTTAAC+GTCGAC) double digest, 420 thousand fragments (389418+31396 sites), 7087 bp average fragment length
   5% of sequence in fragments shorter than    2482 bp
  10% of sequence in fragments shorter than    3742 bp
  15% of sequence in fragments shorter than    4833 bp
  20% of sequence in fragments shorter than    5860 bp
  25% of sequence in fragments shorter than    6869 bp
  30% of sequence in fragments shorter than    7887 bp
  35% of sequence in fragments shorter than    8918 bp
  40% of sequence in fragments shorter than    9971 bp
  45% of sequence in fragments shorter than   11093 bp
  50% of sequence in fragments shorter than   12294 bp
  55% of sequence in fragments shorter than   13578 bp
  60% of sequence in fragments shorter than   14988 bp
  65% of sequence in fragments shorter than   16580 bp
  70% of sequence in fragments shorter than   18397 bp
  75% of sequence in fragments shorter than   20485 bp
  80% of sequence in fragments shorter than   23117 bp
  85% of sequence in fragments shorter than   26535 bp
  90% of sequence in fragments shorter than   31814 bp
  95% of sequence in fragments shorter than   42658 bp

# KpnI.SnaBI (GGTACC+TACGTA) double digest, 419 thousand fragments (287974+132299 sites), 7096 bp average fragment length
   5% of sequence in fragments shorter than    2362 bp
  10% of sequence in fragments shorter than    3662 bp
  15% of sequence in fragments shorter than    4834 bp
  20% of sequence in fragments shorter than    5940 bp
  25% of sequence in fragments shorter than    6993 bp
  30% of sequence in fragments shorter than    8054 bp
  35% of sequence in fragments shorter than    9139 bp
  40% of sequence in fragments shorter than   10231 bp
  45% of sequence in fragments shorter than   11374 bp
  50% of sequence in fragments shorter than   12577 bp
  55% of sequence in fragments shorter than   13874 bp
  60% of sequence in fragments shorter than   15282 bp
  65% of sequence in fragments shorter than   16834 bp
  70% of sequence in fragments shorter than   18639 bp
  75% of sequence in fragments shorter than   20743 bp
  80% of sequence in fragments shorter than   23251 bp
  85% of sequence in fragments shorter than   26560 bp
  90% of sequence in fragments shorter than   31311 bp
  95% of sequence in fragments shorter than   40765 bp

# NheI.SnaBI (GCTAGC+TACGTA) double digest, 419 thousand fragments (287384+132299 sites), 7106 bp average fragment length
   5% of sequence in fragments shorter than    2384 bp
  10% of sequence in fragments shorter than    3662 bp
  15% of sequence in fragments shorter than    4881 bp
  20% of sequence in fragments shorter than    6017 bp
  25% of sequence in fragments shorter than    7109 bp
  30% of sequence in fragments shorter than    8197 bp
  35% of sequence in fragments shorter than    9292 bp
  40% of sequence in fragments shorter than   10432 bp
  45% of sequence in fragments shorter than   11605 bp
  50% of sequence in fragments shorter than   12859 bp
  55% of sequence in fragments shorter than   14203 bp
  60% of sequence in fragments shorter than   15677 bp
  65% of sequence in fragments shorter than   17321 bp
  70% of sequence in fragments shorter than   19193 bp
  75% of sequence in fragments shorter than   21368 bp
  80% of sequence in fragments shorter than   24041 bp
  85% of sequence in fragments shorter than   27497 bp
  90% of sequence in fragments shorter than   32671 bp
  95% of sequence in fragments shorter than   42578 bp

# AgeI.BamHI (ACCGGT+GGATCC) double digest, 419 thousand fragments (53386+365999 sites), 7111 bp average fragment length
   5% of sequence in fragments shorter than    2446 bp
  10% of sequence in fragments shorter than    3683 bp
  15% of sequence in fragments shorter than    4834 bp
  20% of sequence in fragments shorter than    5905 bp
  25% of sequence in fragments shorter than    6966 bp
  30% of sequence in fragments shorter than    8032 bp
  35% of sequence in fragments shorter than    9122 bp
  40% of sequence in fragments shorter than   10259 bp
  45% of sequence in fragments shorter than   11452 bp
  50% of sequence in fragments shorter than   12730 bp
  55% of sequence in fragments shorter than   14099 bp
  60% of sequence in fragments shorter than   15615 bp
  65% of sequence in fragments shorter than   17298 bp
  70% of sequence in fragments shorter than   19255 bp
  75% of sequence in fragments shorter than   21597 bp
  80% of sequence in fragments shorter than   24460 bp
  85% of sequence in fragments shorter than   28165 bp
  90% of sequence in fragments shorter than   33567 bp
  95% of sequence in fragments shorter than   44312 bp

# PmeI.SapI (GTTTAAAC+GCTCTTC) double digest, 418 thousand fragments (42094+377161 sites), 7113 bp average fragment length
   5% of sequence in fragments shorter than    2470 bp
  10% of sequence in fragments shorter than    3791 bp
  15% of sequence in fragments shorter than    4906 bp
  20% of sequence in fragments shorter than    5951 bp
  25% of sequence in fragments shorter than    6975 bp
  30% of sequence in fragments shorter than    7990 bp
  35% of sequence in fragments shorter than    9034 bp
  40% of sequence in fragments shorter than   10107 bp
  45% of sequence in fragments shorter than   11263 bp
  50% of sequence in fragments shorter than   12465 bp
  55% of sequence in fragments shorter than   13766 bp
  60% of sequence in fragments shorter than   15189 bp
  65% of sequence in fragments shorter than   16733 bp
  70% of sequence in fragments shorter than   18479 bp
  75% of sequence in fragments shorter than   20636 bp
  80% of sequence in fragments shorter than   23228 bp
  85% of sequence in fragments shorter than   26587 bp
  90% of sequence in fragments shorter than   31700 bp
  95% of sequence in fragments shorter than   42048 bp

# SexAI.XhoI (ACCWGGT+CTCGAG) double digest, 418 thousand fragments (297900+121308 sites), 7114 bp average fragment length
   5% of sequence in fragments shorter than    2438 bp
  10% of sequence in fragments shorter than    3747 bp
  15% of sequence in fragments shorter than    4887 bp
  20% of sequence in fragments shorter than    5965 bp
  25% of sequence in fragments shorter than    7027 bp
  30% of sequence in fragments shorter than    8080 bp
  35% of sequence in fragments shorter than    9155 bp
  40% of sequence in fragments shorter than   10268 bp
  45% of sequence in fragments shorter than   11463 bp
  50% of sequence in fragments shorter than   12725 bp
  55% of sequence in fragments shorter than   14103 bp
  60% of sequence in fragments shorter than   15606 bp
  65% of sequence in fragments shorter than   17272 bp
  70% of sequence in fragments shorter than   19209 bp
  75% of sequence in fragments shorter than   21498 bp
  80% of sequence in fragments shorter than   24355 bp
  85% of sequence in fragments shorter than   28024 bp
  90% of sequence in fragments shorter than   33585 bp
  95% of sequence in fragments shorter than   45358 bp

# PmeI.SmaI (GTTTAAAC+CCCGGG) double digest, 418 thousand fragments (42094+376940 sites), 7116 bp average fragment length
   5% of sequence in fragments shorter than    2262 bp
  10% of sequence in fragments shorter than    3705 bp
  15% of sequence in fragments shorter than    5071 bp
  20% of sequence in fragments shorter than    6464 bp
  25% of sequence in fragments shorter than    7892 bp
  30% of sequence in fragments shorter than    9388 bp
  35% of sequence in fragments shorter than   10977 bp
  40% of sequence in fragments shorter than   12686 bp
  45% of sequence in fragments shorter than   14574 bp
  50% of sequence in fragments shorter than   16637 bp
  55% of sequence in fragments shorter than   18967 bp
  60% of sequence in fragments shorter than   21570 bp
  65% of sequence in fragments shorter than   24541 bp
  70% of sequence in fragments shorter than   28095 bp
  75% of sequence in fragments shorter than   32301 bp
  80% of sequence in fragments shorter than   37591 bp
  85% of sequence in fragments shorter than   44553 bp
  90% of sequence in fragments shorter than   55069 bp
  95% of sequence in fragments shorter than   76560 bp

# SgrAI.SpeI (CRCCGGYG+ACTAGT) double digest, 415 thousand fragments (15270+400286 sites), 7177 bp average fragment length
   5% of sequence in fragments shorter than    2467 bp
  10% of sequence in fragments shorter than    3750 bp
  15% of sequence in fragments shorter than    4862 bp
  20% of sequence in fragments shorter than    5905 bp
  25% of sequence in fragments shorter than    6921 bp
  30% of sequence in fragments shorter than    7938 bp
  35% of sequence in fragments shorter than    8983 bp
  40% of sequence in fragments shorter than   10069 bp
  45% of sequence in fragments shorter than   11223 bp
  50% of sequence in fragments shorter than   12434 bp
  55% of sequence in fragments shorter than   13761 bp
  60% of sequence in fragments shorter than   15258 bp
  65% of sequence in fragments shorter than   16904 bp
  70% of sequence in fragments shorter than   18806 bp
  75% of sequence in fragments shorter than   21070 bp
  80% of sequence in fragments shorter than   23883 bp
  85% of sequence in fragments shorter than   27691 bp
  90% of sequence in fragments shorter than   33580 bp
  95% of sequence in fragments shorter than   45773 bp

# NruI.SpeI (TCGCGA+ACTAGT) double digest, 414 thousand fragments (14872+400286 sites), 7184 bp average fragment length
   5% of sequence in fragments shorter than    2464 bp
  10% of sequence in fragments shorter than    3743 bp
  15% of sequence in fragments shorter than    4857 bp
  20% of sequence in fragments shorter than    5897 bp
  25% of sequence in fragments shorter than    6911 bp
  30% of sequence in fragments shorter than    7932 bp
  35% of sequence in fragments shorter than    8973 bp
  40% of sequence in fragments shorter than   10066 bp
  45% of sequence in fragments shorter than   11233 bp
  50% of sequence in fragments shorter than   12450 bp
  55% of sequence in fragments shorter than   13785 bp
  60% of sequence in fragments shorter than   15305 bp
  65% of sequence in fragments shorter than   16954 bp
  70% of sequence in fragments shorter than   18877 bp
  75% of sequence in fragments shorter than   21150 bp
  80% of sequence in fragments shorter than   24078 bp
  85% of sequence in fragments shorter than   27971 bp
  90% of sequence in fragments shorter than   34029 bp
  95% of sequence in fragments shorter than   46932 bp

# PvuI.SpeI (CGATCG+ACTAGT) double digest, 414 thousand fragments (14186+400286 sites), 7196 bp average fragment length
   5% of sequence in fragments shorter than    2456 bp
  10% of sequence in fragments shorter than    3744 bp
  15% of sequence in fragments shorter than    4858 bp
  20% of sequence in fragments shorter than    5902 bp
  25% of sequence in fragments shorter than    6924 bp
  30% of sequence in fragments shorter than    7944 bp
  35% of sequence in fragments shorter than    8994 bp
  40% of sequence in fragments shorter than   10088 bp
  45% of sequence in fragments shorter than   11252 bp
  50% of sequence in fragments shorter than   12472 bp
  55% of sequence in fragments shorter than   13818 bp
  60% of sequence in fragments shorter than   15348 bp
  65% of sequence in fragments shorter than   17023 bp
  70% of sequence in fragments shorter than   18961 bp
  75% of sequence in fragments shorter than   21274 bp
  80% of sequence in fragments shorter than   24204 bp
  85% of sequence in fragments shorter than   28100 bp
  90% of sequence in fragments shorter than   34244 bp
  95% of sequence in fragments shorter than   47291 bp

# FseI.SpeI (GGCCGGCC+ACTAGT) double digest, 413 thousand fragments (13662+400286 sites), 7205 bp average fragment length
   5% of sequence in fragments shorter than    2480 bp
  10% of sequence in fragments shorter than    3767 bp
  15% of sequence in fragments shorter than    4882 bp
  20% of sequence in fragments shorter than    5929 bp
  25% of sequence in fragments shorter than    6946 bp
  30% of sequence in fragments shorter than    7968 bp
  35% of sequence in fragments shorter than    9010 bp
  40% of sequence in fragments shorter than   10097 bp
  45% of sequence in fragments shorter than   11258 bp
  50% of sequence in fragments shorter than   12464 bp
  55% of sequence in fragments shorter than   13785 bp
  60% of sequence in fragments shorter than   15287 bp
  65% of sequence in fragments shorter than   16921 bp
  70% of sequence in fragments shorter than   18812 bp
  75% of sequence in fragments shorter than   21061 bp
  80% of sequence in fragments shorter than   23855 bp
  85% of sequence in fragments shorter than   27621 bp
  90% of sequence in fragments shorter than   33391 bp
  95% of sequence in fragments shorter than   45176 bp

# KpnI.NaeI (GGTACC+GCCGGC) double digest, 413 thousand fragments (287974+125692 sites), 7208 bp average fragment length
   5% of sequence in fragments shorter than    2376 bp
  10% of sequence in fragments shorter than    3839 bp
  15% of sequence in fragments shorter than    5148 bp
  20% of sequence in fragments shorter than    6393 bp
  25% of sequence in fragments shorter than    7615 bp
  30% of sequence in fragments shorter than    8843 bp
  35% of sequence in fragments shorter than   10082 bp
  40% of sequence in fragments shorter than   11383 bp
  45% of sequence in fragments shorter than   12766 bp
  50% of sequence in fragments shorter than   14207 bp
  55% of sequence in fragments shorter than   15772 bp
  60% of sequence in fragments shorter than   17453 bp
  65% of sequence in fragments shorter than   19347 bp
  70% of sequence in fragments shorter than   21520 bp
  75% of sequence in fragments shorter than   24104 bp
  80% of sequence in fragments shorter than   27290 bp
  85% of sequence in fragments shorter than   31435 bp
  90% of sequence in fragments shorter than   37509 bp
  95% of sequence in fragments shorter than   50932 bp

# NaeI.NheI (GCCGGC+GCTAGC) double digest, 412 thousand fragments (125692+287384 sites), 7219 bp average fragment length
   5% of sequence in fragments shorter than    2439 bp
  10% of sequence in fragments shorter than    3856 bp
  15% of sequence in fragments shorter than    5206 bp
  20% of sequence in fragments shorter than    6477 bp
  25% of sequence in fragments shorter than    7701 bp
  30% of sequence in fragments shorter than    8936 bp
  35% of sequence in fragments shorter than   10175 bp
  40% of sequence in fragments shorter than   11449 bp
  45% of sequence in fragments shorter than   12792 bp
  50% of sequence in fragments shorter than   14239 bp
  55% of sequence in fragments shorter than   15799 bp
  60% of sequence in fragments shorter than   17513 bp
  65% of sequence in fragments shorter than   19413 bp
  70% of sequence in fragments shorter than   21549 bp
  75% of sequence in fragments shorter than   24070 bp
  80% of sequence in fragments shorter than   27143 bp
  85% of sequence in fragments shorter than   31097 bp
  90% of sequence in fragments shorter than   37035 bp
  95% of sequence in fragments shorter than   48573 bp

# HpaI.MluI (GTTAAC+ACGCGT) double digest, 410 thousand fragments (389418+21415 sites), 7259 bp average fragment length
   5% of sequence in fragments shorter than    2554 bp
  10% of sequence in fragments shorter than    3836 bp
  15% of sequence in fragments shorter than    4957 bp
  20% of sequence in fragments shorter than    6009 bp
  25% of sequence in fragments shorter than    7037 bp
  30% of sequence in fragments shorter than    8087 bp
  35% of sequence in fragments shorter than    9135 bp
  40% of sequence in fragments shorter than   10218 bp
  45% of sequence in fragments shorter than   11383 bp
  50% of sequence in fragments shorter than   12602 bp
  55% of sequence in fragments shorter than   13915 bp
  60% of sequence in fragments shorter than   15359 bp
  65% of sequence in fragments shorter than   16976 bp
  70% of sequence in fragments shorter than   18805 bp
  75% of sequence in fragments shorter than   20947 bp
  80% of sequence in fragments shorter than   23604 bp
  85% of sequence in fragments shorter than   27064 bp
  90% of sequence in fragments shorter than   32366 bp
  95% of sequence in fragments shorter than   43147 bp

# NotI.SpeI (GCGGCCGC+ACTAGT) double digest, 409 thousand fragments (9795+400286 sites), 7273 bp average fragment length
   5% of sequence in fragments shorter than    2497 bp
  10% of sequence in fragments shorter than    3800 bp
  15% of sequence in fragments shorter than    4930 bp
  20% of sequence in fragments shorter than    5988 bp
  25% of sequence in fragments shorter than    7017 bp
  30% of sequence in fragments shorter than    8053 bp
  35% of sequence in fragments shorter than    9109 bp
  40% of sequence in fragments shorter than   10225 bp
  45% of sequence in fragments shorter than   11407 bp
  50% of sequence in fragments shorter than   12635 bp
  55% of sequence in fragments shorter than   13999 bp
  60% of sequence in fragments shorter than   15537 bp
  65% of sequence in fragments shorter than   17222 bp
  70% of sequence in fragments shorter than   19205 bp
  75% of sequence in fragments shorter than   21499 bp
  80% of sequence in fragments shorter than   24448 bp
  85% of sequence in fragments shorter than   28444 bp
  90% of sequence in fragments shorter than   34632 bp
  95% of sequence in fragments shorter than   47712 bp

# RsrII.SpeI (CGGWCCG+ACTAGT) double digest, 409 thousand fragments (9764+400286 sites), 7273 bp average fragment length
   5% of sequence in fragments shorter than    2496 bp
  10% of sequence in fragments shorter than    3794 bp
  15% of sequence in fragments shorter than    4921 bp
  20% of sequence in fragments shorter than    5971 bp
  25% of sequence in fragments shorter than    6996 bp
  30% of sequence in fragments shorter than    8030 bp
  35% of sequence in fragments shorter than    9089 bp
  40% of sequence in fragments shorter than   10196 bp
  45% of sequence in fragments shorter than   11372 bp
  50% of sequence in fragments shorter than   12601 bp
  55% of sequence in fragments shorter than   13960 bp
  60% of sequence in fragments shorter than   15503 bp
  65% of sequence in fragments shorter than   17167 bp
  70% of sequence in fragments shorter than   19126 bp
  75% of sequence in fragments shorter than   21444 bp
  80% of sequence in fragments shorter than   24389 bp
  85% of sequence in fragments shorter than   28390 bp
  90% of sequence in fragments shorter than   34518 bp
  95% of sequence in fragments shorter than   47632 bp

# KpnI.XhoI (GGTACC+CTCGAG) double digest, 408 thousand fragments (287974+121308 sites), 7286 bp average fragment length
   5% of sequence in fragments shorter than    2376 bp
  10% of sequence in fragments shorter than    3785 bp
  15% of sequence in fragments shorter than    5027 bp
  20% of sequence in fragments shorter than    6183 bp
  25% of sequence in fragments shorter than    7316 bp
  30% of sequence in fragments shorter than    8435 bp
  35% of sequence in fragments shorter than    9574 bp
  40% of sequence in fragments shorter than   10758 bp
  45% of sequence in fragments shorter than   11997 bp
  50% of sequence in fragments shorter than   13302 bp
  55% of sequence in fragments shorter than   14721 bp
  60% of sequence in fragments shorter than   16271 bp
  65% of sequence in fragments shorter than   17972 bp
  70% of sequence in fragments shorter than   19930 bp
  75% of sequence in fragments shorter than   22204 bp
  80% of sequence in fragments shorter than   25065 bp
  85% of sequence in fragments shorter than   28697 bp
  90% of sequence in fragments shorter than   34182 bp
  95% of sequence in fragments shorter than   45602 bp

# NheI.XhoI (GCTAGC+CTCGAG) double digest, 408 thousand fragments (287384+121308 sites), 7298 bp average fragment length
   5% of sequence in fragments shorter than    2345 bp
  10% of sequence in fragments shorter than    3802 bp
  15% of sequence in fragments shorter than    5088 bp
  20% of sequence in fragments shorter than    6267 bp
  25% of sequence in fragments shorter than    7399 bp
  30% of sequence in fragments shorter than    8530 bp
  35% of sequence in fragments shorter than    9689 bp
  40% of sequence in fragments shorter than   10871 bp
  45% of sequence in fragments shorter than   12105 bp
  50% of sequence in fragments shorter than   13413 bp
  55% of sequence in fragments shorter than   14813 bp
  60% of sequence in fragments shorter than   16362 bp
  65% of sequence in fragments shorter than   18091 bp
  70% of sequence in fragments shorter than   20024 bp
  75% of sequence in fragments shorter than   22299 bp
  80% of sequence in fragments shorter than   25111 bp
  85% of sequence in fragments shorter than   28681 bp
  90% of sequence in fragments shorter than   34017 bp
  95% of sequence in fragments shorter than   44439 bp

# SalI.SapI (GTCGAC+GCTCTTC) double digest, 408 thousand fragments (31396+377161 sites), 7300 bp average fragment length
   5% of sequence in fragments shorter than    2520 bp
  10% of sequence in fragments shorter than    3886 bp
  15% of sequence in fragments shorter than    5045 bp
  20% of sequence in fragments shorter than    6127 bp
  25% of sequence in fragments shorter than    7206 bp
  30% of sequence in fragments shorter than    8268 bp
  35% of sequence in fragments shorter than    9354 bp
  40% of sequence in fragments shorter than   10490 bp
  45% of sequence in fragments shorter than   11702 bp
  50% of sequence in fragments shorter than   12964 bp
  55% of sequence in fragments shorter than   14330 bp
  60% of sequence in fragments shorter than   15820 bp
  65% of sequence in fragments shorter than   17457 bp
  70% of sequence in fragments shorter than   19379 bp
  75% of sequence in fragments shorter than   21644 bp
  80% of sequence in fragments shorter than   24438 bp
  85% of sequence in fragments shorter than   28102 bp
  90% of sequence in fragments shorter than   33532 bp
  95% of sequence in fragments shorter than   44919 bp

# SalI.SmaI (GTCGAC+CCCGGG) double digest, 408 thousand fragments (31396+376940 sites), 7303 bp average fragment length
   5% of sequence in fragments shorter than    2291 bp
  10% of sequence in fragments shorter than    3792 bp
  15% of sequence in fragments shorter than    5232 bp
  20% of sequence in fragments shorter than    6710 bp
  25% of sequence in fragments shorter than    8252 bp
  30% of sequence in fragments shorter than    9894 bp
  35% of sequence in fragments shorter than   11660 bp
  40% of sequence in fragments shorter than   13594 bp
  45% of sequence in fragments shorter than   15677 bp
  50% of sequence in fragments shorter than   18034 bp
  55% of sequence in fragments shorter than   20700 bp
  60% of sequence in fragments shorter than   23676 bp
  65% of sequence in fragments shorter than   27166 bp
  70% of sequence in fragments shorter than   31242 bp
  75% of sequence in fragments shorter than   36282 bp
  80% of sequence in fragments shorter than   42391 bp
  85% of sequence in fragments shorter than   50516 bp
  90% of sequence in fragments shorter than   62952 bp
  95% of sequence in fragments shorter than   90159 bp

# AclI.BsrBI (AACGTT+CCGCTC) double digest, 407 thousand fragments (172706+235523 sites), 7307 bp average fragment length
   5% of sequence in fragments shorter than    2455 bp
  10% of sequence in fragments shorter than    4004 bp
  15% of sequence in fragments shorter than    5382 bp
  20% of sequence in fragments shorter than    6694 bp
  25% of sequence in fragments shorter than    7983 bp
  30% of sequence in fragments shorter than    9301 bp
  35% of sequence in fragments shorter than   10627 bp
  40% of sequence in fragments shorter than   12002 bp
  45% of sequence in fragments shorter than   13463 bp
  50% of sequence in fragments shorter than   15025 bp
  55% of sequence in fragments shorter than   16678 bp
  60% of sequence in fragments shorter than   18511 bp
  65% of sequence in fragments shorter than   20573 bp
  70% of sequence in fragments shorter than   22902 bp
  75% of sequence in fragments shorter than   25625 bp
  80% of sequence in fragments shorter than   28958 bp
  85% of sequence in fragments shorter than   33268 bp
  90% of sequence in fragments shorter than   39404 bp
  95% of sequence in fragments shorter than   50234 bp

# BamHI.PmeI (GGATCC+GTTTAAAC) double digest, 407 thousand fragments (365999+42094 sites), 7308 bp average fragment length
   5% of sequence in fragments shorter than    2526 bp
  10% of sequence in fragments shorter than    3855 bp
  15% of sequence in fragments shorter than    5002 bp
  20% of sequence in fragments shorter than    6074 bp
  25% of sequence in fragments shorter than    7120 bp
  30% of sequence in fragments shorter than    8179 bp
  35% of sequence in fragments shorter than    9260 bp
  40% of sequence in fragments shorter than   10380 bp
  45% of sequence in fragments shorter than   11556 bp
  50% of sequence in fragments shorter than   12798 bp
  55% of sequence in fragments shorter than   14121 bp
  60% of sequence in fragments shorter than   15603 bp
  65% of sequence in fragments shorter than   17274 bp
  70% of sequence in fragments shorter than   19131 bp
  75% of sequence in fragments shorter than   21342 bp
  80% of sequence in fragments shorter than   24113 bp
  85% of sequence in fragments shorter than   27653 bp
  90% of sequence in fragments shorter than   32805 bp
  95% of sequence in fragments shorter than   42481 bp

# AatII.BstEII (GACGTC+GGTNACC) double digest, 406 thousand fragments (71089+335865 sites), 7328 bp average fragment length
   5% of sequence in fragments shorter than    2530 bp
  10% of sequence in fragments shorter than    3888 bp
  15% of sequence in fragments shorter than    5064 bp
  20% of sequence in fragments shorter than    6184 bp
  25% of sequence in fragments shorter than    7274 bp
  30% of sequence in fragments shorter than    8378 bp
  35% of sequence in fragments shorter than    9511 bp
  40% of sequence in fragments shorter than   10704 bp
  45% of sequence in fragments shorter than   11940 bp
  50% of sequence in fragments shorter than   13277 bp
  55% of sequence in fragments shorter than   14735 bp
  60% of sequence in fragments shorter than   16316 bp
  65% of sequence in fragments shorter than   18111 bp
  70% of sequence in fragments shorter than   20147 bp
  75% of sequence in fragments shorter than   22535 bp
  80% of sequence in fragments shorter than   25546 bp
  85% of sequence in fragments shorter than   29616 bp
  90% of sequence in fragments shorter than   35526 bp
  95% of sequence in fragments shorter than   47411 bp

# AscI.SpeI (GGCGCGCC+ACTAGT) double digest, 404 thousand fragments (4751+400286 sites), 7363 bp average fragment length
   5% of sequence in fragments shorter than    2519 bp
  10% of sequence in fragments shorter than    3835 bp
  15% of sequence in fragments shorter than    4973 bp
  20% of sequence in fragments shorter than    6044 bp
  25% of sequence in fragments shorter than    7079 bp
  30% of sequence in fragments shorter than    8133 bp
  35% of sequence in fragments shorter than    9209 bp
  40% of sequence in fragments shorter than   10334 bp
  45% of sequence in fragments shorter than   11536 bp
  50% of sequence in fragments shorter than   12786 bp
  55% of sequence in fragments shorter than   14176 bp
  60% of sequence in fragments shorter than   15753 bp
  65% of sequence in fragments shorter than   17469 bp
  70% of sequence in fragments shorter than   19518 bp
  75% of sequence in fragments shorter than   21877 bp
  80% of sequence in fragments shorter than   24977 bp
  85% of sequence in fragments shorter than   29242 bp
  90% of sequence in fragments shorter than   35788 bp
  95% of sequence in fragments shorter than   50532 bp

# HpaI.SgrAI (GTTAAC+CRCCGGYG) double digest, 404 thousand fragments (389418+15270 sites), 7369 bp average fragment length
   5% of sequence in fragments shorter than    2591 bp
  10% of sequence in fragments shorter than    3894 bp
  15% of sequence in fragments shorter than    5032 bp
  20% of sequence in fragments shorter than    6103 bp
  25% of sequence in fragments shorter than    7148 bp
  30% of sequence in fragments shorter than    8219 bp
  35% of sequence in fragments shorter than    9286 bp
  40% of sequence in fragments shorter than   10378 bp
  45% of sequence in fragments shorter than   11563 bp
  50% of sequence in fragments shorter than   12805 bp
  55% of sequence in fragments shorter than   14156 bp
  60% of sequence in fragments shorter than   15611 bp
  65% of sequence in fragments shorter than   17273 bp
  70% of sequence in fragments shorter than   19132 bp
  75% of sequence in fragments shorter than   21321 bp
  80% of sequence in fragments shorter than   24072 bp
  85% of sequence in fragments shorter than   27642 bp
  90% of sequence in fragments shorter than   33125 bp
  95% of sequence in fragments shorter than   44210 bp

# HpaI.NruI (GTTAAC+TCGCGA) double digest, 403 thousand fragments (389418+14872 sites), 7376 bp average fragment length
   5% of sequence in fragments shorter than    2588 bp
  10% of sequence in fragments shorter than    3887 bp
  15% of sequence in fragments shorter than    5025 bp
  20% of sequence in fragments shorter than    6095 bp
  25% of sequence in fragments shorter than    7142 bp
  30% of sequence in fragments shorter than    8206 bp
  35% of sequence in fragments shorter than    9277 bp
  40% of sequence in fragments shorter than   10373 bp
  45% of sequence in fragments shorter than   11555 bp
  50% of sequence in fragments shorter than   12804 bp
  55% of sequence in fragments shorter than   14160 bp
  60% of sequence in fragments shorter than   15644 bp
  65% of sequence in fragments shorter than   17311 bp
  70% of sequence in fragments shorter than   19177 bp
  75% of sequence in fragments shorter than   21388 bp
  80% of sequence in fragments shorter than   24153 bp
  85% of sequence in fragments shorter than   27763 bp
  90% of sequence in fragments shorter than   33413 bp
  95% of sequence in fragments shorter than   44849 bp

# HpaI.PvuI (GTTAAC+CGATCG) double digest, 403 thousand fragments (389418+14186 sites), 7389 bp average fragment length
   5% of sequence in fragments shorter than    2579 bp
  10% of sequence in fragments shorter than    3886 bp
  15% of sequence in fragments shorter than    5034 bp
  20% of sequence in fragments shorter than    6110 bp
  25% of sequence in fragments shorter than    7157 bp
  30% of sequence in fragments shorter than    8230 bp
  35% of sequence in fragments shorter than    9310 bp
  40% of sequence in fragments shorter than   10409 bp
  45% of sequence in fragments shorter than   11596 bp
  50% of sequence in fragments shorter than   12847 bp
  55% of sequence in fragments shorter than   14202 bp
  60% of sequence in fragments shorter than   15686 bp
  65% of sequence in fragments shorter than   17352 bp
  70% of sequence in fragments shorter than   19236 bp
  75% of sequence in fragments shorter than   21473 bp
  80% of sequence in fragments shorter than   24264 bp
  85% of sequence in fragments shorter than   27932 bp
  90% of sequence in fragments shorter than   33507 bp
  95% of sequence in fragments shorter than   44745 bp

# FseI.HpaI (GGCCGGCC+GTTAAC) double digest, 402 thousand fragments (13662+389418 sites), 7398 bp average fragment length
   5% of sequence in fragments shorter than    2604 bp
  10% of sequence in fragments shorter than    3910 bp
  15% of sequence in fragments shorter than    5052 bp
  20% of sequence in fragments shorter than    6126 bp
  25% of sequence in fragments shorter than    7176 bp
  30% of sequence in fragments shorter than    8241 bp
  35% of sequence in fragments shorter than    9311 bp
  40% of sequence in fragments shorter than   10400 bp
  45% of sequence in fragments shorter than   11582 bp
  50% of sequence in fragments shorter than   12831 bp
  55% of sequence in fragments shorter than   14171 bp
  60% of sequence in fragments shorter than   15623 bp
  65% of sequence in fragments shorter than   17269 bp
  70% of sequence in fragments shorter than   19114 bp
  75% of sequence in fragments shorter than   21300 bp
  80% of sequence in fragments shorter than   24025 bp
  85% of sequence in fragments shorter than   27590 bp
  90% of sequence in fragments shorter than   33012 bp
  95% of sequence in fragments shorter than   43958 bp

# AclI.KasI (AACGTT+GGCGCC) double digest, 402 thousand fragments (172706+230381 sites), 7400 bp average fragment length
   5% of sequence in fragments shorter than    2510 bp
  10% of sequence in fragments shorter than    3971 bp
  15% of sequence in fragments shorter than    5269 bp
  20% of sequence in fragments shorter than    6538 bp
  25% of sequence in fragments shorter than    7787 bp
  30% of sequence in fragments shorter than    9053 bp
  35% of sequence in fragments shorter than   10358 bp
  40% of sequence in fragments shorter than   11710 bp
  45% of sequence in fragments shorter than   13158 bp
  50% of sequence in fragments shorter than   14693 bp
  55% of sequence in fragments shorter than   16387 bp
  60% of sequence in fragments shorter than   18206 bp
  65% of sequence in fragments shorter than   20271 bp
  70% of sequence in fragments shorter than   22632 bp
  75% of sequence in fragments shorter than   25332 bp
  80% of sequence in fragments shorter than   28655 bp
  85% of sequence in fragments shorter than   33104 bp
  90% of sequence in fragments shorter than   39503 bp
  95% of sequence in fragments shorter than   51212 bp

# BstEII.SacII (GGTNACC+CCGCGG) double digest, 402 thousand fragments (335865+66901 sites), 7403 bp average fragment length
   5% of sequence in fragments shorter than    2638 bp
  10% of sequence in fragments shorter than    4137 bp
  15% of sequence in fragments shorter than    5444 bp
  20% of sequence in fragments shorter than    6679 bp
  25% of sequence in fragments shorter than    7880 bp
  30% of sequence in fragments shorter than    9109 bp
  35% of sequence in fragments shorter than   10364 bp
  40% of sequence in fragments shorter than   11686 bp
  45% of sequence in fragments shorter than   13087 bp
  50% of sequence in fragments shorter than   14578 bp
  55% of sequence in fragments shorter than   16196 bp
  60% of sequence in fragments shorter than   17985 bp
  65% of sequence in fragments shorter than   19969 bp
  70% of sequence in fragments shorter than   22240 bp
  75% of sequence in fragments shorter than   24943 bp
  80% of sequence in fragments shorter than   28343 bp
  85% of sequence in fragments shorter than   32735 bp
  90% of sequence in fragments shorter than   39329 bp
  95% of sequence in fragments shorter than   52974 bp

# HpaI.NotI (GTTAAC+GCGGCCGC) double digest, 398 thousand fragments (389418+9795 sites), 7470 bp average fragment length
   5% of sequence in fragments shorter than    2625 bp
  10% of sequence in fragments shorter than    3946 bp
  15% of sequence in fragments shorter than    5099 bp
  20% of sequence in fragments shorter than    6191 bp
  25% of sequence in fragments shorter than    7252 bp
  30% of sequence in fragments shorter than    8336 bp
  35% of sequence in fragments shorter than    9432 bp
  40% of sequence in fragments shorter than   10538 bp
  45% of sequence in fragments shorter than   11741 bp
  50% of sequence in fragments shorter than   13006 bp
  55% of sequence in fragments shorter than   14369 bp
  60% of sequence in fragments shorter than   15878 bp
  65% of sequence in fragments shorter than   17580 bp
  70% of sequence in fragments shorter than   19477 bp
  75% of sequence in fragments shorter than   21727 bp
  80% of sequence in fragments shorter than   24521 bp
  85% of sequence in fragments shorter than   28218 bp
  90% of sequence in fragments shorter than   33854 bp
  95% of sequence in fragments shorter than   45501 bp

# AclI.SwaI (AACGTT+ATTTAAAT) double digest, 398 thousand fragments (172706+226602 sites), 7470 bp average fragment length
   5% of sequence in fragments shorter than    2546 bp
  10% of sequence in fragments shorter than    3892 bp
  15% of sequence in fragments shorter than    5106 bp
  20% of sequence in fragments shorter than    6254 bp
  25% of sequence in fragments shorter than    7385 bp
  30% of sequence in fragments shorter than    8517 bp
  35% of sequence in fragments shorter than    9674 bp
  40% of sequence in fragments shorter than   10871 bp
  45% of sequence in fragments shorter than   12151 bp
  50% of sequence in fragments shorter than   13515 bp
  55% of sequence in fragments shorter than   15026 bp
  60% of sequence in fragments shorter than   16738 bp
  65% of sequence in fragments shorter than   18641 bp
  70% of sequence in fragments shorter than   20844 bp
  75% of sequence in fragments shorter than   23403 bp
  80% of sequence in fragments shorter than   26655 bp
  85% of sequence in fragments shorter than   31076 bp
  90% of sequence in fragments shorter than   38047 bp
  95% of sequence in fragments shorter than   51645 bp

# HpaI.RsrII (GTTAAC+CGGWCCG) double digest, 398 thousand fragments (389418+9764 sites), 7471 bp average fragment length
   5% of sequence in fragments shorter than    2621 bp
  10% of sequence in fragments shorter than    3940 bp
  15% of sequence in fragments shorter than    5092 bp
  20% of sequence in fragments shorter than    6178 bp
  25% of sequence in fragments shorter than    7237 bp
  30% of sequence in fragments shorter than    8316 bp
  35% of sequence in fragments shorter than    9405 bp
  40% of sequence in fragments shorter than   10515 bp
  45% of sequence in fragments shorter than   11717 bp
  50% of sequence in fragments shorter than   12978 bp
  55% of sequence in fragments shorter than   14336 bp
  60% of sequence in fragments shorter than   15834 bp
  65% of sequence in fragments shorter than   17525 bp
  70% of sequence in fragments shorter than   19411 bp
  75% of sequence in fragments shorter than   21640 bp
  80% of sequence in fragments shorter than   24447 bp
  85% of sequence in fragments shorter than   28157 bp
  90% of sequence in fragments shorter than   33799 bp
  95% of sequence in fragments shorter than   45539 bp

# MluI.SapI (ACGCGT+GCTCTTC) double digest, 398 thousand fragments (21415+377161 sites), 7482 bp average fragment length
   5% of sequence in fragments shorter than    2585 bp
  10% of sequence in fragments shorter than    3985 bp
  15% of sequence in fragments shorter than    5174 bp
  20% of sequence in fragments shorter than    6287 bp
  25% of sequence in fragments shorter than    7391 bp
  30% of sequence in fragments shorter than    8483 bp
  35% of sequence in fragments shorter than    9596 bp
  40% of sequence in fragments shorter than   10766 bp
  45% of sequence in fragments shorter than   12009 bp
  50% of sequence in fragments shorter than   13332 bp
  55% of sequence in fragments shorter than   14725 bp
  60% of sequence in fragments shorter than   16269 bp
  65% of sequence in fragments shorter than   17962 bp
  70% of sequence in fragments shorter than   19930 bp
  75% of sequence in fragments shorter than   22248 bp
  80% of sequence in fragments shorter than   25161 bp
  85% of sequence in fragments shorter than   28879 bp
  90% of sequence in fragments shorter than   34490 bp
  95% of sequence in fragments shorter than   46436 bp

# MluI.SmaI (ACGCGT+CCCGGG) double digest, 398 thousand fragments (21415+376940 sites), 7486 bp average fragment length
   5% of sequence in fragments shorter than    2331 bp
  10% of sequence in fragments shorter than    3879 bp
  15% of sequence in fragments shorter than    5384 bp
  20% of sequence in fragments shorter than    6948 bp
  25% of sequence in fragments shorter than    8595 bp
  30% of sequence in fragments shorter than   10348 bp
  35% of sequence in fragments shorter than   12248 bp
  40% of sequence in fragments shorter than   14349 bp
  45% of sequence in fragments shorter than   16668 bp
  50% of sequence in fragments shorter than   19269 bp
  55% of sequence in fragments shorter than   22203 bp
  60% of sequence in fragments shorter than   25501 bp
  65% of sequence in fragments shorter than   29408 bp
  70% of sequence in fragments shorter than   34007 bp
  75% of sequence in fragments shorter than   39484 bp
  80% of sequence in fragments shorter than   46360 bp
  85% of sequence in fragments shorter than   55738 bp
  90% of sequence in fragments shorter than   70404 bp
  95% of sequence in fragments shorter than  101535 bp

# BamHI.SalI (GGATCC+GTCGAC) double digest, 397 thousand fragments (365999+31396 sites), 7505 bp average fragment length
   5% of sequence in fragments shorter than    2580 bp
  10% of sequence in fragments shorter than    3948 bp
  15% of sequence in fragments shorter than    5139 bp
  20% of sequence in fragments shorter than    6266 bp
  25% of sequence in fragments shorter than    7369 bp
  30% of sequence in fragments shorter than    8479 bp
  35% of sequence in fragments shorter than    9623 bp
  40% of sequence in fragments shorter than   10807 bp
  45% of sequence in fragments shorter than   12059 bp
  50% of sequence in fragments shorter than   13367 bp
  55% of sequence in fragments shorter than   14826 bp
  60% of sequence in fragments shorter than   16405 bp
  65% of sequence in fragments shorter than   18159 bp
  70% of sequence in fragments shorter than   20210 bp
  75% of sequence in fragments shorter than   22657 bp
  80% of sequence in fragments shorter than   25636 bp
  85% of sequence in fragments shorter than   29461 bp
  90% of sequence in fragments shorter than   35081 bp
  95% of sequence in fragments shorter than   45944 bp

# BstEII.PspXI (GGTNACC+VCTCGAGB) double digest, 394 thousand fragments (335865+59193 sites), 7548 bp average fragment length
   5% of sequence in fragments shorter than    2602 bp
  10% of sequence in fragments shorter than    4001 bp
  15% of sequence in fragments shorter than    5213 bp
  20% of sequence in fragments shorter than    6376 bp
  25% of sequence in fragments shorter than    7500 bp
  30% of sequence in fragments shorter than    8627 bp
  35% of sequence in fragments shorter than    9797 bp
  40% of sequence in fragments shorter than   11016 bp
  45% of sequence in fragments shorter than   12307 bp
  50% of sequence in fragments shorter than   13712 bp
  55% of sequence in fragments shorter than   15201 bp
  60% of sequence in fragments shorter than   16833 bp
  65% of sequence in fragments shorter than   18689 bp
  70% of sequence in fragments shorter than   20796 bp
  75% of sequence in fragments shorter than   23283 bp
  80% of sequence in fragments shorter than   26455 bp
  85% of sequence in fragments shorter than   30603 bp
  90% of sequence in fragments shorter than   36739 bp
  95% of sequence in fragments shorter than   49602 bp

# BsrBI.PacI (CCGCTC+TTAATTAA) double digest, 394 thousand fragments (235523+159455 sites), 7552 bp average fragment length
   5% of sequence in fragments shorter than    2733 bp
  10% of sequence in fragments shorter than    4299 bp
  15% of sequence in fragments shorter than    5683 bp
  20% of sequence in fragments shorter than    6996 bp
  25% of sequence in fragments shorter than    8281 bp
  30% of sequence in fragments shorter than    9584 bp
  35% of sequence in fragments shorter than   10920 bp
  40% of sequence in fragments shorter than   12305 bp
  45% of sequence in fragments shorter than   13766 bp
  50% of sequence in fragments shorter than   15296 bp
  55% of sequence in fragments shorter than   16926 bp
  60% of sequence in fragments shorter than   18750 bp
  65% of sequence in fragments shorter than   20781 bp
  70% of sequence in fragments shorter than   23095 bp
  75% of sequence in fragments shorter than   25829 bp
  80% of sequence in fragments shorter than   29054 bp
  85% of sequence in fragments shorter than   33208 bp
  90% of sequence in fragments shorter than   39191 bp
  95% of sequence in fragments shorter than   50674 bp

# AscI.HpaI (GGCGCGCC+GTTAAC) double digest, 393 thousand fragments (4751+389418 sites), 7566 bp average fragment length
   5% of sequence in fragments shorter than    2651 bp
  10% of sequence in fragments shorter than    3984 bp
  15% of sequence in fragments shorter than    5151 bp
  20% of sequence in fragments shorter than    6252 bp
  25% of sequence in fragments shorter than    7330 bp
  30% of sequence in fragments shorter than    8424 bp
  35% of sequence in fragments shorter than    9526 bp
  40% of sequence in fragments shorter than   10662 bp
  45% of sequence in fragments shorter than   11880 bp
  50% of sequence in fragments shorter than   13158 bp
  55% of sequence in fragments shorter than   14550 bp
  60% of sequence in fragments shorter than   16089 bp
  65% of sequence in fragments shorter than   17826 bp
  70% of sequence in fragments shorter than   19750 bp
  75% of sequence in fragments shorter than   22098 bp
  80% of sequence in fragments shorter than   24938 bp
  85% of sequence in fragments shorter than   28749 bp
  90% of sequence in fragments shorter than   34706 bp
  95% of sequence in fragments shorter than   47245 bp

# BsrBI.Hpy99I (CCGCTC+CGWCG) double digest, 393 thousand fragments (235523+157917 sites), 7582 bp average fragment length
   5% of sequence in fragments shorter than    2650 bp
  10% of sequence in fragments shorter than    4572 bp
  15% of sequence in fragments shorter than    6430 bp
  20% of sequence in fragments shorter than    8283 bp
  25% of sequence in fragments shorter than   10173 bp
  30% of sequence in fragments shorter than   12120 bp
  35% of sequence in fragments shorter than   14225 bp
  40% of sequence in fragments shorter than   16413 bp
  45% of sequence in fragments shorter than   18766 bp
  50% of sequence in fragments shorter than   21350 bp
  55% of sequence in fragments shorter than   24221 bp
  60% of sequence in fragments shorter than   27525 bp
  65% of sequence in fragments shorter than   31128 bp
  70% of sequence in fragments shorter than   35469 bp
  75% of sequence in fragments shorter than   40360 bp
  80% of sequence in fragments shorter than   46473 bp
  85% of sequence in fragments shorter than   54628 bp
  90% of sequence in fragments shorter than   66463 bp
  95% of sequence in fragments shorter than   88073 bp

# SapI.SgrAI (GCTCTTC+CRCCGGYG) double digest, 392 thousand fragments (377161+15270 sites), 7599 bp average fragment length
   5% of sequence in fragments shorter than    2627 bp
  10% of sequence in fragments shorter than    4058 bp
  15% of sequence in fragments shorter than    5269 bp
  20% of sequence in fragments shorter than    6396 bp
  25% of sequence in fragments shorter than    7523 bp
  30% of sequence in fragments shorter than    8636 bp
  35% of sequence in fragments shorter than    9775 bp
  40% of sequence in fragments shorter than   10972 bp
  45% of sequence in fragments shorter than   12224 bp
  50% of sequence in fragments shorter than   13555 bp
  55% of sequence in fragments shorter than   14976 bp
  60% of sequence in fragments shorter than   16533 bp
  65% of sequence in fragments shorter than   18267 bp
  70% of sequence in fragments shorter than   20273 bp
  75% of sequence in fragments shorter than   22654 bp
  80% of sequence in fragments shorter than   25582 bp
  85% of sequence in fragments shorter than   29464 bp
  90% of sequence in fragments shorter than   35214 bp
  95% of sequence in fragments shorter than   47519 bp

# SgrAI.SmaI (CRCCGGYG+CCCGGG) double digest, 391 thousand fragments (15270+376940 sites), 7602 bp average fragment length
   5% of sequence in fragments shorter than    2384 bp
  10% of sequence in fragments shorter than    3976 bp
  15% of sequence in fragments shorter than    5523 bp
  20% of sequence in fragments shorter than    7140 bp
  25% of sequence in fragments shorter than    8841 bp
  30% of sequence in fragments shorter than   10637 bp
  35% of sequence in fragments shorter than   12598 bp
  40% of sequence in fragments shorter than   14774 bp
  45% of sequence in fragments shorter than   17152 bp
  50% of sequence in fragments shorter than   19859 bp
  55% of sequence in fragments shorter than   22900 bp
  60% of sequence in fragments shorter than   26324 bp
  65% of sequence in fragments shorter than   30342 bp
  70% of sequence in fragments shorter than   35035 bp
  75% of sequence in fragments shorter than   40885 bp
  80% of sequence in fragments shorter than   47836 bp
  85% of sequence in fragments shorter than   57614 bp
  90% of sequence in fragments shorter than   72702 bp
  95% of sequence in fragments shorter than  106429 bp

# NruI.SapI (TCGCGA+GCTCTTC) double digest, 391 thousand fragments (14872+377161 sites), 7607 bp average fragment length
   5% of sequence in fragments shorter than    2631 bp
  10% of sequence in fragments shorter than    4054 bp
  15% of sequence in fragments shorter than    5258 bp
  20% of sequence in fragments shorter than    6380 bp
  25% of sequence in fragments shorter than    7497 bp
  30% of sequence in fragments shorter than    8610 bp
  35% of sequence in fragments shorter than    9750 bp
  40% of sequence in fragments shorter than   10936 bp
  45% of sequence in fragments shorter than   12181 bp
  50% of sequence in fragments shorter than   13501 bp
  55% of sequence in fragments shorter than   14916 bp
  60% of sequence in fragments shorter than   16454 bp
  65% of sequence in fragments shorter than   18151 bp
  70% of sequence in fragments shorter than   20120 bp
  75% of sequence in fragments shorter than   22468 bp
  80% of sequence in fragments shorter than   25352 bp
  85% of sequence in fragments shorter than   29162 bp
  90% of sequence in fragments shorter than   34856 bp
  95% of sequence in fragments shorter than   46917 bp

# NruI.SmaI (TCGCGA+CCCGGG) double digest, 391 thousand fragments (14872+376940 sites), 7610 bp average fragment length
   5% of sequence in fragments shorter than    2376 bp
  10% of sequence in fragments shorter than    3952 bp
  15% of sequence in fragments shorter than    5490 bp
  20% of sequence in fragments shorter than    7088 bp
  25% of sequence in fragments shorter than    8751 bp
  30% of sequence in fragments shorter than   10543 bp
  35% of sequence in fragments shorter than   12476 bp
  40% of sequence in fragments shorter than   14625 bp
  45% of sequence in fragments shorter than   16953 bp
  50% of sequence in fragments shorter than   19619 bp
  55% of sequence in fragments shorter than   22615 bp
  60% of sequence in fragments shorter than   25979 bp
  65% of sequence in fragments shorter than   29957 bp
  70% of sequence in fragments shorter than   34628 bp
  75% of sequence in fragments shorter than   40283 bp
  80% of sequence in fragments shorter than   47108 bp
  85% of sequence in fragments shorter than   56639 bp
  90% of sequence in fragments shorter than   71283 bp
  95% of sequence in fragments shorter than  104631 bp

# PvuI.SapI (CGATCG+GCTCTTC) double digest, 391 thousand fragments (14186+377161 sites), 7621 bp average fragment length
   5% of sequence in fragments shorter than    2620 bp
  10% of sequence in fragments shorter than    4052 bp
  15% of sequence in fragments shorter than    5266 bp
  20% of sequence in fragments shorter than    6395 bp
  25% of sequence in fragments shorter than    7522 bp
  30% of sequence in fragments shorter than    8630 bp
  35% of sequence in fragments shorter than    9772 bp
  40% of sequence in fragments shorter than   10968 bp
  45% of sequence in fragments shorter than   12216 bp
  50% of sequence in fragments shorter than   13545 bp
  55% of sequence in fragments shorter than   14964 bp
  60% of sequence in fragments shorter than   16512 bp
  65% of sequence in fragments shorter than   18225 bp
  70% of sequence in fragments shorter than   20207 bp
  75% of sequence in fragments shorter than   22558 bp
  80% of sequence in fragments shorter than   25457 bp
  85% of sequence in fragments shorter than   29240 bp
  90% of sequence in fragments shorter than   34886 bp
  95% of sequence in fragments shorter than   46767 bp

# PvuI.SmaI (CGATCG+CCCGGG) double digest, 390 thousand fragments (14186+376940 sites), 7624 bp average fragment length
   5% of sequence in fragments shorter than    2360 bp
  10% of sequence in fragments shorter than    3935 bp
  15% of sequence in fragments shorter than    5467 bp
  20% of sequence in fragments shorter than    7062 bp
  25% of sequence in fragments shorter than    8728 bp
  30% of sequence in fragments shorter than   10513 bp
  35% of sequence in fragments shorter than   12443 bp
  40% of sequence in fragments shorter than   14589 bp
  45% of sequence in fragments shorter than   16926 bp
  50% of sequence in fragments shorter than   19569 bp
  55% of sequence in fragments shorter than   22537 bp
  60% of sequence in fragments shorter than   25896 bp
  65% of sequence in fragments shorter than   29836 bp
  70% of sequence in fragments shorter than   34381 bp
  75% of sequence in fragments shorter than   39929 bp
  80% of sequence in fragments shorter than   46786 bp
  85% of sequence in fragments shorter than   55966 bp
  90% of sequence in fragments shorter than   70292 bp
  95% of sequence in fragments shorter than  100938 bp

# FseI.SapI (GGCCGGCC+GCTCTTC) double digest, 390 thousand fragments (13662+377161 sites), 7631 bp average fragment length
   5% of sequence in fragments shorter than    2635 bp
  10% of sequence in fragments shorter than    4067 bp
  15% of sequence in fragments shorter than    5280 bp
  20% of sequence in fragments shorter than    6414 bp
  25% of sequence in fragments shorter than    7546 bp
  30% of sequence in fragments shorter than    8667 bp
  35% of sequence in fragments shorter than    9821 bp
  40% of sequence in fragments shorter than   11022 bp
  45% of sequence in fragments shorter than   12281 bp
  50% of sequence in fragments shorter than   13621 bp
  55% of sequence in fragments shorter than   15066 bp
  60% of sequence in fragments shorter than   16627 bp
  65% of sequence in fragments shorter than   18352 bp
  70% of sequence in fragments shorter than   20385 bp
  75% of sequence in fragments shorter than   22760 bp
  80% of sequence in fragments shorter than   25710 bp
  85% of sequence in fragments shorter than   29581 bp
  90% of sequence in fragments shorter than   35367 bp
  95% of sequence in fragments shorter than   47605 bp

# FseI.SmaI (GGCCGGCC+CCCGGG) double digest, 390 thousand fragments (13662+376940 sites), 7634 bp average fragment length
   5% of sequence in fragments shorter than    2381 bp
  10% of sequence in fragments shorter than    3978 bp
  15% of sequence in fragments shorter than    5532 bp
  20% of sequence in fragments shorter than    7160 bp
  25% of sequence in fragments shorter than    8860 bp
  30% of sequence in fragments shorter than   10681 bp
  35% of sequence in fragments shorter than   12656 bp
  40% of sequence in fragments shorter than   14838 bp
  45% of sequence in fragments shorter than   17249 bp
  50% of sequence in fragments shorter than   19979 bp
  55% of sequence in fragments shorter than   23045 bp
  60% of sequence in fragments shorter than   26558 bp
  65% of sequence in fragments shorter than   30628 bp
  70% of sequence in fragments shorter than   35404 bp
  75% of sequence in fragments shorter than   41301 bp
  80% of sequence in fragments shorter than   48330 bp
  85% of sequence in fragments shorter than   58152 bp
  90% of sequence in fragments shorter than   73468 bp
  95% of sequence in fragments shorter than  107917 bp

# KasI.PacI (GGCGCC+TTAATTAA) double digest, 389 thousand fragments (230381+159455 sites), 7649 bp average fragment length
   5% of sequence in fragments shorter than    2740 bp
  10% of sequence in fragments shorter than    4278 bp
  15% of sequence in fragments shorter than    5640 bp
  20% of sequence in fragments shorter than    6930 bp
  25% of sequence in fragments shorter than    8202 bp
  30% of sequence in fragments shorter than    9483 bp
  35% of sequence in fragments shorter than   10815 bp
  40% of sequence in fragments shorter than   12194 bp
  45% of sequence in fragments shorter than   13688 bp
  50% of sequence in fragments shorter than   15285 bp
  55% of sequence in fragments shorter than   16984 bp
  60% of sequence in fragments shorter than   18867 bp
  65% of sequence in fragments shorter than   21010 bp
  70% of sequence in fragments shorter than   23433 bp
  75% of sequence in fragments shorter than   26275 bp
  80% of sequence in fragments shorter than   29754 bp
  85% of sequence in fragments shorter than   34425 bp
  90% of sequence in fragments shorter than   41251 bp
  95% of sequence in fragments shorter than   55560 bp

# AgeI.BstEII (ACCGGT+GGTNACC) double digest, 388 thousand fragments (53386+335865 sites), 7661 bp average fragment length
   5% of sequence in fragments shorter than    2681 bp
  10% of sequence in fragments shorter than    4108 bp
  15% of sequence in fragments shorter than    5331 bp
  20% of sequence in fragments shorter than    6489 bp
  25% of sequence in fragments shorter than    7625 bp
  30% of sequence in fragments shorter than    8761 bp
  35% of sequence in fragments shorter than    9955 bp
  40% of sequence in fragments shorter than   11175 bp
  45% of sequence in fragments shorter than   12466 bp
  50% of sequence in fragments shorter than   13869 bp
  55% of sequence in fragments shorter than   15338 bp
  60% of sequence in fragments shorter than   17006 bp
  65% of sequence in fragments shorter than   18852 bp
  70% of sequence in fragments shorter than   20954 bp
  75% of sequence in fragments shorter than   23426 bp
  80% of sequence in fragments shorter than   26615 bp
  85% of sequence in fragments shorter than   30688 bp
  90% of sequence in fragments shorter than   36714 bp
  95% of sequence in fragments shorter than   49304 bp

# Hpy99I.KasI (CGWCG+GGCGCC) double digest, 387 thousand fragments (157917+230381 sites), 7681 bp average fragment length
   5% of sequence in fragments shorter than    2697 bp
  10% of sequence in fragments shorter than    4531 bp
  15% of sequence in fragments shorter than    6274 bp
  20% of sequence in fragments shorter than    8013 bp
  25% of sequence in fragments shorter than    9816 bp
  30% of sequence in fragments shorter than   11705 bp
  35% of sequence in fragments shorter than   13735 bp
  40% of sequence in fragments shorter than   15926 bp
  45% of sequence in fragments shorter than   18274 bp
  50% of sequence in fragments shorter than   20837 bp
  55% of sequence in fragments shorter than   23691 bp
  60% of sequence in fragments shorter than   26902 bp
  65% of sequence in fragments shorter than   30596 bp
  70% of sequence in fragments shorter than   35002 bp
  75% of sequence in fragments shorter than   40159 bp
  80% of sequence in fragments shorter than   46688 bp
  85% of sequence in fragments shorter than   55102 bp
  90% of sequence in fragments shorter than   67712 bp
  95% of sequence in fragments shorter than   91526 bp

# BamHI.MluI (GGATCC+ACGCGT) double digest, 387 thousand fragments (365999+21415 sites), 7698 bp average fragment length
   5% of sequence in fragments shorter than    2641 bp
  10% of sequence in fragments shorter than    4055 bp
  15% of sequence in fragments shorter than    5284 bp
  20% of sequence in fragments shorter than    6439 bp
  25% of sequence in fragments shorter than    7577 bp
  30% of sequence in fragments shorter than    8721 bp
  35% of sequence in fragments shorter than    9915 bp
  40% of sequence in fragments shorter than   11120 bp
  45% of sequence in fragments shorter than   12420 bp
  50% of sequence in fragments shorter than   13793 bp
  55% of sequence in fragments shorter than   15298 bp
  60% of sequence in fragments shorter than   16943 bp
  65% of sequence in fragments shorter than   18780 bp
  70% of sequence in fragments shorter than   20907 bp
  75% of sequence in fragments shorter than   23432 bp
  80% of sequence in fragments shorter than   26529 bp
  85% of sequence in fragments shorter than   30570 bp
  90% of sequence in fragments shorter than   36419 bp
  95% of sequence in fragments shorter than   47811 bp

# NotI.SapI (GCGGCCGC+GCTCTTC) double digest, 386 thousand fragments (9795+377161 sites), 7707 bp average fragment length
   5% of sequence in fragments shorter than    2667 bp
  10% of sequence in fragments shorter than    4122 bp
  15% of sequence in fragments shorter than    5349 bp
  20% of sequence in fragments shorter than    6498 bp
  25% of sequence in fragments shorter than    7640 bp
  30% of sequence in fragments shorter than    8761 bp
  35% of sequence in fragments shorter than    9923 bp
  40% of sequence in fragments shorter than   11140 bp
  45% of sequence in fragments shorter than   12411 bp
  50% of sequence in fragments shorter than   13755 bp
  55% of sequence in fragments shorter than   15213 bp
  60% of sequence in fragments shorter than   16790 bp
  65% of sequence in fragments shorter than   18525 bp
  70% of sequence in fragments shorter than   20590 bp
  75% of sequence in fragments shorter than   22972 bp
  80% of sequence in fragments shorter than   25965 bp
  85% of sequence in fragments shorter than   29870 bp
  90% of sequence in fragments shorter than   35698 bp
  95% of sequence in fragments shorter than   48265 bp

# RsrII.SapI (CGGWCCG+GCTCTTC) double digest, 386 thousand fragments (9764+377161 sites), 7708 bp average fragment length
   5% of sequence in fragments shorter than    2662 bp
  10% of sequence in fragments shorter than    4111 bp
  15% of sequence in fragments shorter than    5335 bp
  20% of sequence in fragments shorter than    6477 bp
  25% of sequence in fragments shorter than    7619 bp
  30% of sequence in fragments shorter than    8742 bp
  35% of sequence in fragments shorter than    9903 bp
  40% of sequence in fragments shorter than   11118 bp
  45% of sequence in fragments shorter than   12383 bp
  50% of sequence in fragments shorter than   13720 bp
  55% of sequence in fragments shorter than   15185 bp
  60% of sequence in fragments shorter than   16753 bp
  65% of sequence in fragments shorter than   18491 bp
  70% of sequence in fragments shorter than   20535 bp
  75% of sequence in fragments shorter than   22915 bp
  80% of sequence in fragments shorter than   25891 bp
  85% of sequence in fragments shorter than   29781 bp
  90% of sequence in fragments shorter than   35545 bp
  95% of sequence in fragments shorter than   47793 bp

# NotI.SmaI (GCGGCCGC+CCCGGG) double digest, 386 thousand fragments (9795+376940 sites), 7710 bp average fragment length
   5% of sequence in fragments shorter than    2421 bp
  10% of sequence in fragments shorter than    4043 bp
  15% of sequence in fragments shorter than    5616 bp
  20% of sequence in fragments shorter than    7268 bp
  25% of sequence in fragments shorter than    8989 bp
  30% of sequence in fragments shorter than   10830 bp
  35% of sequence in fragments shorter than   12829 bp
  40% of sequence in fragments shorter than   15043 bp
  45% of sequence in fragments shorter than   17483 bp
  50% of sequence in fragments shorter than   20250 bp
  55% of sequence in fragments shorter than   23337 bp
  60% of sequence in fragments shorter than   26914 bp
  65% of sequence in fragments shorter than   30968 bp
  70% of sequence in fragments shorter than   35836 bp
  75% of sequence in fragments shorter than   41789 bp
  80% of sequence in fragments shorter than   48885 bp
  85% of sequence in fragments shorter than   58761 bp
  90% of sequence in fragments shorter than   74129 bp
  95% of sequence in fragments shorter than  109155 bp

# RsrII.SmaI (CGGWCCG+CCCGGG) double digest, 386 thousand fragments (9764+376940 sites), 7711 bp average fragment length
   5% of sequence in fragments shorter than    2404 bp
  10% of sequence in fragments shorter than    4017 bp
  15% of sequence in fragments shorter than    5576 bp
  20% of sequence in fragments shorter than    7210 bp
  25% of sequence in fragments shorter than    8927 bp
  30% of sequence in fragments shorter than   10742 bp
  35% of sequence in fragments shorter than   12724 bp
  40% of sequence in fragments shorter than   14906 bp
  45% of sequence in fragments shorter than   17300 bp
  50% of sequence in fragments shorter than   20033 bp
  55% of sequence in fragments shorter than   23105 bp
  60% of sequence in fragments shorter than   26610 bp
  65% of sequence in fragments shorter than   30656 bp
  70% of sequence in fragments shorter than   35458 bp
  75% of sequence in fragments shorter than   41369 bp
  80% of sequence in fragments shorter than   48441 bp
  85% of sequence in fragments shorter than   58207 bp
  90% of sequence in fragments shorter than   73215 bp
  95% of sequence in fragments shorter than  107212 bp

# PacI.SwaI (TTAATTAA+ATTTAAAT) double digest, 385 thousand fragments (159455+226602 sites), 7724 bp average fragment length
   5% of sequence in fragments shorter than    2693 bp
  10% of sequence in fragments shorter than    4142 bp
  15% of sequence in fragments shorter than    5428 bp
  20% of sequence in fragments shorter than    6678 bp
  25% of sequence in fragments shorter than    7936 bp
  30% of sequence in fragments shorter than    9174 bp
  35% of sequence in fragments shorter than   10459 bp
  40% of sequence in fragments shorter than   11817 bp
  45% of sequence in fragments shorter than   13281 bp
  50% of sequence in fragments shorter than   14878 bp
  55% of sequence in fragments shorter than   16681 bp
  60% of sequence in fragments shorter than   18678 bp
  65% of sequence in fragments shorter than   20988 bp
  70% of sequence in fragments shorter than   23726 bp
  75% of sequence in fragments shorter than   27117 bp
  80% of sequence in fragments shorter than   31561 bp
  85% of sequence in fragments shorter than   38355 bp
  90% of sequence in fragments shorter than   49033 bp
  95% of sequence in fragments shorter than   77258 bp

# Hpy99I.SwaI (CGWCG+ATTTAAAT) double digest, 384 thousand fragments (157917+226602 sites), 7757 bp average fragment length
   5% of sequence in fragments shorter than    2824 bp
  10% of sequence in fragments shorter than    4380 bp
  15% of sequence in fragments shorter than    5738 bp
  20% of sequence in fragments shorter than    7009 bp
  25% of sequence in fragments shorter than    8259 bp
  30% of sequence in fragments shorter than    9488 bp
  35% of sequence in fragments shorter than   10741 bp
  40% of sequence in fragments shorter than   12039 bp
  45% of sequence in fragments shorter than   13405 bp
  50% of sequence in fragments shorter than   14855 bp
  55% of sequence in fragments shorter than   16453 bp
  60% of sequence in fragments shorter than   18205 bp
  65% of sequence in fragments shorter than   20121 bp
  70% of sequence in fragments shorter than   22320 bp
  75% of sequence in fragments shorter than   24922 bp
  80% of sequence in fragments shorter than   28122 bp
  85% of sequence in fragments shorter than   32176 bp
  90% of sequence in fragments shorter than   38289 bp
  95% of sequence in fragments shorter than   50421 bp

# ClaI.SexAI (ATCGAT+ACCWGGT) double digest, 384 thousand fragments (86477+297900 sites), 7759 bp average fragment length
   5% of sequence in fragments shorter than    2708 bp
  10% of sequence in fragments shorter than    4093 bp
  15% of sequence in fragments shorter than    5308 bp
  20% of sequence in fragments shorter than    6456 bp
  25% of sequence in fragments shorter than    7558 bp
  30% of sequence in fragments shorter than    8662 bp
  35% of sequence in fragments shorter than    9785 bp
  40% of sequence in fragments shorter than   10948 bp
  45% of sequence in fragments shorter than   12183 bp
  50% of sequence in fragments shorter than   13481 bp
  55% of sequence in fragments shorter than   14893 bp
  60% of sequence in fragments shorter than   16380 bp
  65% of sequence in fragments shorter than   18053 bp
  70% of sequence in fragments shorter than   19963 bp
  75% of sequence in fragments shorter than   22239 bp
  80% of sequence in fragments shorter than   24998 bp
  85% of sequence in fragments shorter than   28631 bp
  90% of sequence in fragments shorter than   33901 bp
  95% of sequence in fragments shorter than   44853 bp

# AscI.SapI (GGCGCGCC+GCTCTTC) double digest, 381 thousand fragments (4751+377161 sites), 7809 bp average fragment length
   5% of sequence in fragments shorter than    2702 bp
  10% of sequence in fragments shorter than    4167 bp
  15% of sequence in fragments shorter than    5401 bp
  20% of sequence in fragments shorter than    6559 bp
  25% of sequence in fragments shorter than    7711 bp
  30% of sequence in fragments shorter than    8844 bp
  35% of sequence in fragments shorter than   10008 bp
  40% of sequence in fragments shorter than   11238 bp
  45% of sequence in fragments shorter than   12508 bp
  50% of sequence in fragments shorter than   13868 bp
  55% of sequence in fragments shorter than   15327 bp
  60% of sequence in fragments shorter than   16899 bp
  65% of sequence in fragments shorter than   18660 bp
  70% of sequence in fragments shorter than   20742 bp
  75% of sequence in fragments shorter than   23149 bp
  80% of sequence in fragments shorter than   26135 bp
  85% of sequence in fragments shorter than   30075 bp
  90% of sequence in fragments shorter than   35908 bp
  95% of sequence in fragments shorter than   48551 bp

# AscI.SmaI (GGCGCGCC+CCCGGG) double digest, 381 thousand fragments (4751+376940 sites), 7812 bp average fragment length
   5% of sequence in fragments shorter than    2438 bp
  10% of sequence in fragments shorter than    4063 bp
  15% of sequence in fragments shorter than    5639 bp
  20% of sequence in fragments shorter than    7293 bp
  25% of sequence in fragments shorter than    9017 bp
  30% of sequence in fragments shorter than   10866 bp
  35% of sequence in fragments shorter than   12862 bp
  40% of sequence in fragments shorter than   15078 bp
  45% of sequence in fragments shorter than   17519 bp
  50% of sequence in fragments shorter than   20299 bp
  55% of sequence in fragments shorter than   23393 bp
  60% of sequence in fragments shorter than   26965 bp
  65% of sequence in fragments shorter than   31029 bp
  70% of sequence in fragments shorter than   35905 bp
  75% of sequence in fragments shorter than   41869 bp
  80% of sequence in fragments shorter than   49000 bp
  85% of sequence in fragments shorter than   58852 bp
  90% of sequence in fragments shorter than   74324 bp
  95% of sequence in fragments shorter than  109708 bp

# BamHI.SgrAI (GGATCC+CRCCGGYG) double digest, 380 thousand fragments (365999+15270 sites), 7822 bp average fragment length
   5% of sequence in fragments shorter than    2687 bp
  10% of sequence in fragments shorter than    4131 bp
  15% of sequence in fragments shorter than    5389 bp
  20% of sequence in fragments shorter than    6570 bp
  25% of sequence in fragments shorter than    7742 bp
  30% of sequence in fragments shorter than    8905 bp
  35% of sequence in fragments shorter than   10114 bp
  40% of sequence in fragments shorter than   11359 bp
  45% of sequence in fragments shorter than   12681 bp
  50% of sequence in fragments shorter than   14077 bp
  55% of sequence in fragments shorter than   15619 bp
  60% of sequence in fragments shorter than   17291 bp
  65% of sequence in fragments shorter than   19188 bp
  70% of sequence in fragments shorter than   21391 bp
  75% of sequence in fragments shorter than   23994 bp
  80% of sequence in fragments shorter than   27160 bp
  85% of sequence in fragments shorter than   31306 bp
  90% of sequence in fragments shorter than   37257 bp
  95% of sequence in fragments shorter than   48969 bp

# BamHI.NruI (GGATCC+TCGCGA) double digest, 380 thousand fragments (365999+14872 sites), 7830 bp average fragment length
   5% of sequence in fragments shorter than    2690 bp
  10% of sequence in fragments shorter than    4122 bp
  15% of sequence in fragments shorter than    5369 bp
  20% of sequence in fragments shorter than    6542 bp
  25% of sequence in fragments shorter than    7701 bp
  30% of sequence in fragments shorter than    8860 bp
  35% of sequence in fragments shorter than   10063 bp
  40% of sequence in fragments shorter than   11291 bp
  45% of sequence in fragments shorter than   12600 bp
  50% of sequence in fragments shorter than   13989 bp
  55% of sequence in fragments shorter than   15510 bp
  60% of sequence in fragments shorter than   17153 bp
  65% of sequence in fragments shorter than   19024 bp
  70% of sequence in fragments shorter than   21164 bp
  75% of sequence in fragments shorter than   23716 bp
  80% of sequence in fragments shorter than   26833 bp
  85% of sequence in fragments shorter than   30911 bp
  90% of sequence in fragments shorter than   36762 bp
  95% of sequence in fragments shorter than   48385 bp

# BamHI.PvuI (GGATCC+CGATCG) double digest, 379 thousand fragments (365999+14186 sites), 7844 bp average fragment length
   5% of sequence in fragments shorter than    2679 bp
  10% of sequence in fragments shorter than    4121 bp
  15% of sequence in fragments shorter than    5372 bp
  20% of sequence in fragments shorter than    6547 bp
  25% of sequence in fragments shorter than    7710 bp
  30% of sequence in fragments shorter than    8873 bp
  35% of sequence in fragments shorter than   10082 bp
  40% of sequence in fragments shorter than   11316 bp
  45% of sequence in fragments shorter than   12628 bp
  50% of sequence in fragments shorter than   14010 bp
  55% of sequence in fragments shorter than   15535 bp
  60% of sequence in fragments shorter than   17187 bp
  65% of sequence in fragments shorter than   19062 bp
  70% of sequence in fragments shorter than   21200 bp
  75% of sequence in fragments shorter than   23755 bp
  80% of sequence in fragments shorter than   26885 bp
  85% of sequence in fragments shorter than   30969 bp
  90% of sequence in fragments shorter than   36804 bp
  95% of sequence in fragments shorter than   48171 bp

# BamHI.FseI (GGATCC+GGCCGGCC) double digest, 379 thousand fragments (365999+13662 sites), 7855 bp average fragment length
   5% of sequence in fragments shorter than    2693 bp
  10% of sequence in fragments shorter than    4142 bp
  15% of sequence in fragments shorter than    5403 bp
  20% of sequence in fragments shorter than    6587 bp
  25% of sequence in fragments shorter than    7761 bp
  30% of sequence in fragments shorter than    8933 bp
  35% of sequence in fragments shorter than   10154 bp
  40% of sequence in fragments shorter than   11407 bp
  45% of sequence in fragments shorter than   12737 bp
  50% of sequence in fragments shorter than   14144 bp
  55% of sequence in fragments shorter than   15692 bp
  60% of sequence in fragments shorter than   17366 bp
  65% of sequence in fragments shorter than   19273 bp
  70% of sequence in fragments shorter than   21482 bp
  75% of sequence in fragments shorter than   24094 bp
  80% of sequence in fragments shorter than   27298 bp
  85% of sequence in fragments shorter than   31477 bp
  90% of sequence in fragments shorter than   37503 bp
  95% of sequence in fragments shorter than   49192 bp

# BstEII.PmeI (GGTNACC+GTTTAAAC) double digest, 377 thousand fragments (335865+42094 sites), 7890 bp average fragment length
   5% of sequence in fragments shorter than    2764 bp
  10% of sequence in fragments shorter than    4202 bp
  15% of sequence in fragments shorter than    5431 bp
  20% of sequence in fragments shorter than    6601 bp
  25% of sequence in fragments shorter than    7730 bp
  30% of sequence in fragments shorter than    8871 bp
  35% of sequence in fragments shorter than   10033 bp
  40% of sequence in fragments shorter than   11243 bp
  45% of sequence in fragments shorter than   12494 bp
  50% of sequence in fragments shorter than   13868 bp
  55% of sequence in fragments shorter than   15307 bp
  60% of sequence in fragments shorter than   16916 bp
  65% of sequence in fragments shorter than   18731 bp
  70% of sequence in fragments shorter than   20758 bp
  75% of sequence in fragments shorter than   23153 bp
  80% of sequence in fragments shorter than   26114 bp
  85% of sequence in fragments shorter than   30008 bp
  90% of sequence in fragments shorter than   35807 bp
  95% of sequence in fragments shorter than   47688 bp

# BamHI.NotI (GGATCC+GCGGCCGC) double digest, 375 thousand fragments (365999+9795 sites), 7936 bp average fragment length
   5% of sequence in fragments shorter than    2730 bp
  10% of sequence in fragments shorter than    4194 bp
  15% of sequence in fragments shorter than    5469 bp
  20% of sequence in fragments shorter than    6658 bp
  25% of sequence in fragments shorter than    7845 bp
  30% of sequence in fragments shorter than    9031 bp
  35% of sequence in fragments shorter than   10256 bp
  40% of sequence in fragments shorter than   11521 bp
  45% of sequence in fragments shorter than   12860 bp
  50% of sequence in fragments shorter than   14281 bp
  55% of sequence in fragments shorter than   15827 bp
  60% of sequence in fragments shorter than   17508 bp
  65% of sequence in fragments shorter than   19423 bp
  70% of sequence in fragments shorter than   21657 bp
  75% of sequence in fragments shorter than   24279 bp
  80% of sequence in fragments shorter than   27460 bp
  85% of sequence in fragments shorter than   31643 bp
  90% of sequence in fragments shorter than   37742 bp
  95% of sequence in fragments shorter than   49479 bp

# BamHI.RsrII (GGATCC+CGGWCCG) double digest, 375 thousand fragments (365999+9764 sites), 7937 bp average fragment length
   5% of sequence in fragments shorter than    2726 bp
  10% of sequence in fragments shorter than    4187 bp
  15% of sequence in fragments shorter than    5451 bp
  20% of sequence in fragments shorter than    6639 bp
  25% of sequence in fragments shorter than    7822 bp
  30% of sequence in fragments shorter than    9004 bp
  35% of sequence in fragments shorter than   10222 bp
  40% of sequence in fragments shorter than   11485 bp
  45% of sequence in fragments shorter than   12812 bp
  50% of sequence in fragments shorter than   14222 bp
  55% of sequence in fragments shorter than   15767 bp
  60% of sequence in fragments shorter than   17443 bp
  65% of sequence in fragments shorter than   19352 bp
  70% of sequence in fragments shorter than   21573 bp
  75% of sequence in fragments shorter than   24186 bp
  80% of sequence in fragments shorter than   27369 bp
  85% of sequence in fragments shorter than   31551 bp
  90% of sequence in fragments shorter than   37586 bp
  95% of sequence in fragments shorter than   49260 bp

# ClaI.KpnI (ATCGAT+GGTACC) double digest, 374 thousand fragments (86477+287974 sites), 7965 bp average fragment length
   5% of sequence in fragments shorter than    2648 bp
  10% of sequence in fragments shorter than    4162 bp
  15% of sequence in fragments shorter than    5506 bp
  20% of sequence in fragments shorter than    6746 bp
  25% of sequence in fragments shorter than    7944 bp
  30% of sequence in fragments shorter than    9141 bp
  35% of sequence in fragments shorter than   10373 bp
  40% of sequence in fragments shorter than   11631 bp
  45% of sequence in fragments shorter than   12955 bp
  50% of sequence in fragments shorter than   14334 bp
  55% of sequence in fragments shorter than   15768 bp
  60% of sequence in fragments shorter than   17371 bp
  65% of sequence in fragments shorter than   19172 bp
  70% of sequence in fragments shorter than   21242 bp
  75% of sequence in fragments shorter than   23577 bp
  80% of sequence in fragments shorter than   26507 bp
  85% of sequence in fragments shorter than   30326 bp
  90% of sequence in fragments shorter than   35940 bp
  95% of sequence in fragments shorter than   47053 bp

# ClaI.NheI (ATCGAT+GCTAGC) double digest, 373 thousand fragments (86477+287384 sites), 7978 bp average fragment length
   5% of sequence in fragments shorter than    2638 bp
  10% of sequence in fragments shorter than    4179 bp
  15% of sequence in fragments shorter than    5570 bp
  20% of sequence in fragments shorter than    6866 bp
  25% of sequence in fragments shorter than    8098 bp
  30% of sequence in fragments shorter than    9340 bp
  35% of sequence in fragments shorter than   10599 bp
  40% of sequence in fragments shorter than   11856 bp
  45% of sequence in fragments shorter than   13174 bp
  50% of sequence in fragments shorter than   14584 bp
  55% of sequence in fragments shorter than   16123 bp
  60% of sequence in fragments shorter than   17778 bp
  65% of sequence in fragments shorter than   19610 bp
  70% of sequence in fragments shorter than   21721 bp
  75% of sequence in fragments shorter than   24199 bp
  80% of sequence in fragments shorter than   27193 bp
  85% of sequence in fragments shorter than   31093 bp
  90% of sequence in fragments shorter than   36875 bp
  95% of sequence in fragments shorter than   48106 bp

# AscI.BamHI (GGCGCGCC+GGATCC) double digest, 370 thousand fragments (4751+365999 sites), 8044 bp average fragment length
   5% of sequence in fragments shorter than    2766 bp
  10% of sequence in fragments shorter than    4239 bp
  15% of sequence in fragments shorter than    5522 bp
  20% of sequence in fragments shorter than    6720 bp
  25% of sequence in fragments shorter than    7914 bp
  30% of sequence in fragments shorter than    9105 bp
  35% of sequence in fragments shorter than   10328 bp
  40% of sequence in fragments shorter than   11604 bp
  45% of sequence in fragments shorter than   12948 bp
  50% of sequence in fragments shorter than   14379 bp
  55% of sequence in fragments shorter than   15929 bp
  60% of sequence in fragments shorter than   17611 bp
  65% of sequence in fragments shorter than   19535 bp
  70% of sequence in fragments shorter than   21768 bp
  75% of sequence in fragments shorter than   24395 bp
  80% of sequence in fragments shorter than   27586 bp
  85% of sequence in fragments shorter than   31805 bp
  90% of sequence in fragments shorter than   37859 bp
  95% of sequence in fragments shorter than   49688 bp

# AatII.SexAI (GACGTC+ACCWGGT) double digest, 368 thousand fragments (71089+297900 sites), 8083 bp average fragment length
   5% of sequence in fragments shorter than    2782 bp
  10% of sequence in fragments shorter than    4251 bp
  15% of sequence in fragments shorter than    5554 bp
  20% of sequence in fragments shorter than    6785 bp
  25% of sequence in fragments shorter than    7981 bp
  30% of sequence in fragments shorter than    9191 bp
  35% of sequence in fragments shorter than   10434 bp
  40% of sequence in fragments shorter than   11731 bp
  45% of sequence in fragments shorter than   13077 bp
  50% of sequence in fragments shorter than   14521 bp
  55% of sequence in fragments shorter than   16097 bp
  60% of sequence in fragments shorter than   17787 bp
  65% of sequence in fragments shorter than   19683 bp
  70% of sequence in fragments shorter than   21863 bp
  75% of sequence in fragments shorter than   24449 bp
  80% of sequence in fragments shorter than   27556 bp
  85% of sequence in fragments shorter than   31782 bp
  90% of sequence in fragments shorter than   37973 bp
  95% of sequence in fragments shorter than   50594 bp

# BsrBI.SnaBI (CCGCTC+TACGTA) double digest, 367 thousand fragments (235523+132299 sites), 8111 bp average fragment length
   5% of sequence in fragments shorter than    2830 bp
  10% of sequence in fragments shorter than    4536 bp
  15% of sequence in fragments shorter than    6062 bp
  20% of sequence in fragments shorter than    7508 bp
  25% of sequence in fragments shorter than    8921 bp
  30% of sequence in fragments shorter than   10380 bp
  35% of sequence in fragments shorter than   11864 bp
  40% of sequence in fragments shorter than   13401 bp
  45% of sequence in fragments shorter than   15045 bp
  50% of sequence in fragments shorter than   16775 bp
  55% of sequence in fragments shorter than   18635 bp
  60% of sequence in fragments shorter than   20743 bp
  65% of sequence in fragments shorter than   23070 bp
  70% of sequence in fragments shorter than   25736 bp
  75% of sequence in fragments shorter than   28834 bp
  80% of sequence in fragments shorter than   32505 bp
  85% of sequence in fragments shorter than   37370 bp
  90% of sequence in fragments shorter than   44349 bp
  95% of sequence in fragments shorter than   57255 bp

# BstEII.SalI (GGTNACC+GTCGAC) double digest, 366 thousand fragments (335865+31396 sites), 8121 bp average fragment length
   5% of sequence in fragments shorter than    2828 bp
  10% of sequence in fragments shorter than    4319 bp
  15% of sequence in fragments shorter than    5602 bp
  20% of sequence in fragments shorter than    6824 bp
  25% of sequence in fragments shorter than    8003 bp
  30% of sequence in fragments shorter than    9212 bp
  35% of sequence in fragments shorter than   10448 bp
  40% of sequence in fragments shorter than   11725 bp
  45% of sequence in fragments shorter than   13084 bp
  50% of sequence in fragments shorter than   14534 bp
  55% of sequence in fragments shorter than   16093 bp
  60% of sequence in fragments shorter than   17821 bp
  65% of sequence in fragments shorter than   19732 bp
  70% of sequence in fragments shorter than   21949 bp
  75% of sequence in fragments shorter than   24564 bp
  80% of sequence in fragments shorter than   27826 bp
  85% of sequence in fragments shorter than   32088 bp
  90% of sequence in fragments shorter than   38420 bp
  95% of sequence in fragments shorter than   51428 bp

# SacII.SexAI (CCGCGG+ACCWGGT) double digest, 364 thousand fragments (66901+297900 sites), 8175 bp average fragment length
   5% of sequence in fragments shorter than    2899 bp
  10% of sequence in fragments shorter than    4528 bp
  15% of sequence in fragments shorter than    5973 bp
  20% of sequence in fragments shorter than    7330 bp
  25% of sequence in fragments shorter than    8651 bp
  30% of sequence in fragments shorter than    9988 bp
  35% of sequence in fragments shorter than   11370 bp
  40% of sequence in fragments shorter than   12810 bp
  45% of sequence in fragments shorter than   14327 bp
  50% of sequence in fragments shorter than   15934 bp
  55% of sequence in fragments shorter than   17693 bp
  60% of sequence in fragments shorter than   19610 bp
  65% of sequence in fragments shorter than   21742 bp
  70% of sequence in fragments shorter than   24200 bp
  75% of sequence in fragments shorter than   27049 bp
  80% of sequence in fragments shorter than   30664 bp
  85% of sequence in fragments shorter than   35492 bp
  90% of sequence in fragments shorter than   42492 bp
  95% of sequence in fragments shorter than   57880 bp

# KasI.SnaBI (GGCGCC+TACGTA) double digest, 362 thousand fragments (230381+132299 sites), 8224 bp average fragment length
   5% of sequence in fragments shorter than    2834 bp
  10% of sequence in fragments shorter than    4500 bp
  15% of sequence in fragments shorter than    5962 bp
  20% of sequence in fragments shorter than    7358 bp
  25% of sequence in fragments shorter than    8778 bp
  30% of sequence in fragments shorter than   10201 bp
  35% of sequence in fragments shorter than   11663 bp
  40% of sequence in fragments shorter than   13206 bp
  45% of sequence in fragments shorter than   14875 bp
  50% of sequence in fragments shorter than   16657 bp
  55% of sequence in fragments shorter than   18534 bp
  60% of sequence in fragments shorter than   20716 bp
  65% of sequence in fragments shorter than   23119 bp
  70% of sequence in fragments shorter than   25868 bp
  75% of sequence in fragments shorter than   29148 bp
  80% of sequence in fragments shorter than   33209 bp
  85% of sequence in fragments shorter than   38598 bp
  90% of sequence in fragments shorter than   46425 bp
  95% of sequence in fragments shorter than   62259 bp

# BsrBI.NaeI (CCGCTC+GCCGGC) double digest, 360 thousand fragments (235523+125692 sites), 8259 bp average fragment length
   5% of sequence in fragments shorter than    2870 bp
  10% of sequence in fragments shorter than    5026 bp
  15% of sequence in fragments shorter than    7105 bp
  20% of sequence in fragments shorter than    9220 bp
  25% of sequence in fragments shorter than   11401 bp
  30% of sequence in fragments shorter than   13674 bp
  35% of sequence in fragments shorter than   16092 bp
  40% of sequence in fragments shorter than   18700 bp
  45% of sequence in fragments shorter than   21468 bp
  50% of sequence in fragments shorter than   24517 bp
  55% of sequence in fragments shorter than   27955 bp
  60% of sequence in fragments shorter than   31658 bp
  65% of sequence in fragments shorter than   35888 bp
  70% of sequence in fragments shorter than   40840 bp
  75% of sequence in fragments shorter than   46782 bp
  80% of sequence in fragments shorter than   54007 bp
  85% of sequence in fragments shorter than   63665 bp
  90% of sequence in fragments shorter than   77518 bp
  95% of sequence in fragments shorter than  105735 bp

# AatII.KpnI (GACGTC+GGTACC) double digest, 358 thousand fragments (71089+287974 sites), 8307 bp average fragment length
   5% of sequence in fragments shorter than    2755 bp
  10% of sequence in fragments shorter than    4313 bp
  15% of sequence in fragments shorter than    5705 bp
  20% of sequence in fragments shorter than    6990 bp
  25% of sequence in fragments shorter than    8245 bp
  30% of sequence in fragments shorter than    9477 bp
  35% of sequence in fragments shorter than   10762 bp
  40% of sequence in fragments shorter than   12084 bp
  45% of sequence in fragments shorter than   13458 bp
  50% of sequence in fragments shorter than   14906 bp
  55% of sequence in fragments shorter than   16452 bp
  60% of sequence in fragments shorter than   18187 bp
  65% of sequence in fragments shorter than   20118 bp
  70% of sequence in fragments shorter than   22290 bp
  75% of sequence in fragments shorter than   24893 bp
  80% of sequence in fragments shorter than   28040 bp
  85% of sequence in fragments shorter than   32122 bp
  90% of sequence in fragments shorter than   38172 bp
  95% of sequence in fragments shorter than   50341 bp

# SnaBI.SwaI (TACGTA+ATTTAAAT) double digest, 358 thousand fragments (132299+226602 sites), 8310 bp average fragment length
   5% of sequence in fragments shorter than    2830 bp
  10% of sequence in fragments shorter than    4367 bp
  15% of sequence in fragments shorter than    5738 bp
  20% of sequence in fragments shorter than    7054 bp
  25% of sequence in fragments shorter than    8351 bp
  30% of sequence in fragments shorter than    9649 bp
  35% of sequence in fragments shorter than   10967 bp
  40% of sequence in fragments shorter than   12358 bp
  45% of sequence in fragments shorter than   13862 bp
  50% of sequence in fragments shorter than   15502 bp
  55% of sequence in fragments shorter than   17296 bp
  60% of sequence in fragments shorter than   19306 bp
  65% of sequence in fragments shorter than   21647 bp
  70% of sequence in fragments shorter than   24380 bp
  75% of sequence in fragments shorter than   27648 bp
  80% of sequence in fragments shorter than   31836 bp
  85% of sequence in fragments shorter than   37864 bp
  90% of sequence in fragments shorter than   47485 bp
  95% of sequence in fragments shorter than   69887 bp

# AatII.NheI (GACGTC+GCTAGC) double digest, 358 thousand fragments (71089+287384 sites), 8321 bp average fragment length
   5% of sequence in fragments shorter than    2744 bp
  10% of sequence in fragments shorter than    4337 bp
  15% of sequence in fragments shorter than    5772 bp
  20% of sequence in fragments shorter than    7107 bp
  25% of sequence in fragments shorter than    8377 bp
  30% of sequence in fragments shorter than    9667 bp
  35% of sequence in fragments shorter than   10963 bp
  40% of sequence in fragments shorter than   12264 bp
  45% of sequence in fragments shorter than   13664 bp
  50% of sequence in fragments shorter than   15133 bp
  55% of sequence in fragments shorter than   16723 bp
  60% of sequence in fragments shorter than   18462 bp
  65% of sequence in fragments shorter than   20359 bp
  70% of sequence in fragments shorter than   22502 bp
  75% of sequence in fragments shorter than   25078 bp
  80% of sequence in fragments shorter than   28192 bp
  85% of sequence in fragments shorter than   32252 bp
  90% of sequence in fragments shorter than   37975 bp
  95% of sequence in fragments shorter than   48730 bp

# BstEII.MluI (GGTNACC+ACGCGT) double digest, 356 thousand fragments (335865+21415 sites), 8347 bp average fragment length
   5% of sequence in fragments shorter than    2898 bp
  10% of sequence in fragments shorter than    4435 bp
  15% of sequence in fragments shorter than    5753 bp
  20% of sequence in fragments shorter than    7020 bp
  25% of sequence in fragments shorter than    8254 bp
  30% of sequence in fragments shorter than    9497 bp
  35% of sequence in fragments shorter than   10777 bp
  40% of sequence in fragments shorter than   12126 bp
  45% of sequence in fragments shorter than   13548 bp
  50% of sequence in fragments shorter than   15050 bp
  55% of sequence in fragments shorter than   16680 bp
  60% of sequence in fragments shorter than   18483 bp
  65% of sequence in fragments shorter than   20491 bp
  70% of sequence in fragments shorter than   22742 bp
  75% of sequence in fragments shorter than   25543 bp
  80% of sequence in fragments shorter than   28959 bp
  85% of sequence in fragments shorter than   33385 bp
  90% of sequence in fragments shorter than   40076 bp
  95% of sequence in fragments shorter than   53978 bp

# PspXI.SexAI (VCTCGAGB+ACCWGGT) double digest, 356 thousand fragments (59193+297900 sites), 8352 bp average fragment length
   5% of sequence in fragments shorter than    2858 bp
  10% of sequence in fragments shorter than    4384 bp
  15% of sequence in fragments shorter than    5726 bp
  20% of sequence in fragments shorter than    6999 bp
  25% of sequence in fragments shorter than    8235 bp
  30% of sequence in fragments shorter than    9478 bp
  35% of sequence in fragments shorter than   10758 bp
  40% of sequence in fragments shorter than   12107 bp
  45% of sequence in fragments shorter than   13486 bp
  50% of sequence in fragments shorter than   15001 bp
  55% of sequence in fragments shorter than   16611 bp
  60% of sequence in fragments shorter than   18395 bp
  65% of sequence in fragments shorter than   20392 bp
  70% of sequence in fragments shorter than   22686 bp
  75% of sequence in fragments shorter than   25360 bp
  80% of sequence in fragments shorter than   28715 bp
  85% of sequence in fragments shorter than   33103 bp
  90% of sequence in fragments shorter than   39613 bp
  95% of sequence in fragments shorter than   53612 bp

# BsrBI.XhoI (CCGCTC+CTCGAG) double digest, 356 thousand fragments (235523+121308 sites), 8361 bp average fragment length
   5% of sequence in fragments shorter than    2778 bp
  10% of sequence in fragments shorter than    4643 bp
  15% of sequence in fragments shorter than    6360 bp
  20% of sequence in fragments shorter than    8031 bp
  25% of sequence in fragments shorter than    9756 bp
  30% of sequence in fragments shorter than   11522 bp
  35% of sequence in fragments shorter than   13360 bp
  40% of sequence in fragments shorter than   15318 bp
  45% of sequence in fragments shorter than   17366 bp
  50% of sequence in fragments shorter than   19685 bp
  55% of sequence in fragments shorter than   22171 bp
  60% of sequence in fragments shorter than   24987 bp
  65% of sequence in fragments shorter than   28214 bp
  70% of sequence in fragments shorter than   31889 bp
  75% of sequence in fragments shorter than   36276 bp
  80% of sequence in fragments shorter than   41868 bp
  85% of sequence in fragments shorter than   48822 bp
  90% of sequence in fragments shorter than   59149 bp
  95% of sequence in fragments shorter than   78850 bp

# KasI.NaeI (GGCGCC+GCCGGC) double digest, 355 thousand fragments (230381+125692 sites), 8375 bp average fragment length
   5% of sequence in fragments shorter than    2911 bp
  10% of sequence in fragments shorter than    4932 bp
  15% of sequence in fragments shorter than    6867 bp
  20% of sequence in fragments shorter than    8840 bp
  25% of sequence in fragments shorter than   10892 bp
  30% of sequence in fragments shorter than   13068 bp
  35% of sequence in fragments shorter than   15430 bp
  40% of sequence in fragments shorter than   17963 bp
  45% of sequence in fragments shorter than   20777 bp
  50% of sequence in fragments shorter than   23891 bp
  55% of sequence in fragments shorter than   27306 bp
  60% of sequence in fragments shorter than   31174 bp
  65% of sequence in fragments shorter than   35696 bp
  70% of sequence in fragments shorter than   40882 bp
  75% of sequence in fragments shorter than   47248 bp
  80% of sequence in fragments shorter than   55158 bp
  85% of sequence in fragments shorter than   65535 bp
  90% of sequence in fragments shorter than   81318 bp
  95% of sequence in fragments shorter than  115981 bp

# KpnI.SacII (GGTACC+CCGCGG) double digest, 354 thousand fragments (287974+66901 sites), 8404 bp average fragment length
   5% of sequence in fragments shorter than    2857 bp
  10% of sequence in fragments shorter than    4635 bp
  15% of sequence in fragments shorter than    6174 bp
  20% of sequence in fragments shorter than    7623 bp
  25% of sequence in fragments shorter than    9039 bp
  30% of sequence in fragments shorter than   10428 bp
  35% of sequence in fragments shorter than   11855 bp
  40% of sequence in fragments shorter than   13331 bp
  45% of sequence in fragments shorter than   14872 bp
  50% of sequence in fragments shorter than   16509 bp
  55% of sequence in fragments shorter than   18285 bp
  60% of sequence in fragments shorter than   20211 bp
  65% of sequence in fragments shorter than   22328 bp
  70% of sequence in fragments shorter than   24764 bp
  75% of sequence in fragments shorter than   27583 bp
  80% of sequence in fragments shorter than   31093 bp
  85% of sequence in fragments shorter than   35697 bp
  90% of sequence in fragments shorter than   42608 bp
  95% of sequence in fragments shorter than   56642 bp

# NheI.SacII (GCTAGC+CCGCGG) double digest, 353 thousand fragments (287384+66901 sites), 8419 bp average fragment length
   5% of sequence in fragments shorter than    2790 bp
  10% of sequence in fragments shorter than    4678 bp
  15% of sequence in fragments shorter than    6289 bp
  20% of sequence in fragments shorter than    7768 bp
  25% of sequence in fragments shorter than    9217 bp
  30% of sequence in fragments shorter than   10642 bp
  35% of sequence in fragments shorter than   12033 bp
  40% of sequence in fragments shorter than   13539 bp
  45% of sequence in fragments shorter than   15091 bp
  50% of sequence in fragments shorter than   16749 bp
  55% of sequence in fragments shorter than   18538 bp
  60% of sequence in fragments shorter than   20462 bp
  65% of sequence in fragments shorter than   22590 bp
  70% of sequence in fragments shorter than   25050 bp
  75% of sequence in fragments shorter than   27871 bp
  80% of sequence in fragments shorter than   31370 bp
  85% of sequence in fragments shorter than   35862 bp
  90% of sequence in fragments shorter than   42468 bp
  95% of sequence in fragments shorter than   55742 bp

# NaeI.SwaI (GCCGGC+ATTTAAAT) double digest, 352 thousand fragments (125692+226602 sites), 8465 bp average fragment length
   5% of sequence in fragments shorter than    3060 bp
  10% of sequence in fragments shorter than    4723 bp
  15% of sequence in fragments shorter than    6178 bp
  20% of sequence in fragments shorter than    7571 bp
  25% of sequence in fragments shorter than    8933 bp
  30% of sequence in fragments shorter than   10270 bp
  35% of sequence in fragments shorter than   11648 bp
  40% of sequence in fragments shorter than   13059 bp
  45% of sequence in fragments shorter than   14533 bp
  50% of sequence in fragments shorter than   16142 bp
  55% of sequence in fragments shorter than   17859 bp
  60% of sequence in fragments shorter than   19736 bp
  65% of sequence in fragments shorter than   21879 bp
  70% of sequence in fragments shorter than   24319 bp
  75% of sequence in fragments shorter than   27149 bp
  80% of sequence in fragments shorter than   30748 bp
  85% of sequence in fragments shorter than   35382 bp
  90% of sequence in fragments shorter than   42272 bp
  95% of sequence in fragments shorter than   57478 bp

# KasI.XhoI (GGCGCC+CTCGAG) double digest, 351 thousand fragments (230381+121308 sites), 8481 bp average fragment length
   5% of sequence in fragments shorter than    2826 bp
  10% of sequence in fragments shorter than    4601 bp
  15% of sequence in fragments shorter than    6220 bp
  20% of sequence in fragments shorter than    7831 bp
  25% of sequence in fragments shorter than    9470 bp
  30% of sequence in fragments shorter than   11163 bp
  35% of sequence in fragments shorter than   12994 bp
  40% of sequence in fragments shorter than   14953 bp
  45% of sequence in fragments shorter than   17069 bp
  50% of sequence in fragments shorter than   19350 bp
  55% of sequence in fragments shorter than   21887 bp
  60% of sequence in fragments shorter than   24765 bp
  65% of sequence in fragments shorter than   27990 bp
  70% of sequence in fragments shorter than   31845 bp
  75% of sequence in fragments shorter than   36414 bp
  80% of sequence in fragments shorter than   42089 bp
  85% of sequence in fragments shorter than   49841 bp
  90% of sequence in fragments shorter than   61011 bp
  95% of sequence in fragments shorter than   85009 bp

# AgeI.SexAI (ACCGGT+ACCWGGT) double digest, 350 thousand fragments (53386+297900 sites), 8490 bp average fragment length
   5% of sequence in fragments shorter than    2932 bp
  10% of sequence in fragments shorter than    4479 bp
  15% of sequence in fragments shorter than    5824 bp
  20% of sequence in fragments shorter than    7081 bp
  25% of sequence in fragments shorter than    8304 bp
  30% of sequence in fragments shorter than    9557 bp
  35% of sequence in fragments shorter than   10822 bp
  40% of sequence in fragments shorter than   12151 bp
  45% of sequence in fragments shorter than   13515 bp
  50% of sequence in fragments shorter than   14990 bp
  55% of sequence in fragments shorter than   16563 bp
  60% of sequence in fragments shorter than   18320 bp
  65% of sequence in fragments shorter than   20237 bp
  70% of sequence in fragments shorter than   22452 bp
  75% of sequence in fragments shorter than   25073 bp
  80% of sequence in fragments shorter than   28230 bp
  85% of sequence in fragments shorter than   32429 bp
  90% of sequence in fragments shorter than   38814 bp
  95% of sequence in fragments shorter than   52431 bp

# BstEII.SgrAI (GGTNACC+CRCCGGYG) double digest, 350 thousand fragments (335865+15270 sites), 8493 bp average fragment length
   5% of sequence in fragments shorter than    2956 bp
  10% of sequence in fragments shorter than    4530 bp
  15% of sequence in fragments shorter than    5884 bp
  20% of sequence in fragments shorter than    7157 bp
  25% of sequence in fragments shorter than    8420 bp
  30% of sequence in fragments shorter than    9682 bp
  35% of sequence in fragments shorter than   10990 bp
  40% of sequence in fragments shorter than   12355 bp
  45% of sequence in fragments shorter than   13812 bp
  50% of sequence in fragments shorter than   15324 bp
  55% of sequence in fragments shorter than   17008 bp
  60% of sequence in fragments shorter than   18844 bp
  65% of sequence in fragments shorter than   20885 bp
  70% of sequence in fragments shorter than   23217 bp
  75% of sequence in fragments shorter than   26079 bp
  80% of sequence in fragments shorter than   29516 bp
  85% of sequence in fragments shorter than   34060 bp
  90% of sequence in fragments shorter than   40895 bp
  95% of sequence in fragments shorter than   55349 bp

# BstEII.NruI (GGTNACC+TCGCGA) double digest, 350 thousand fragments (335865+14872 sites), 8503 bp average fragment length
   5% of sequence in fragments shorter than    2958 bp
  10% of sequence in fragments shorter than    4520 bp
  15% of sequence in fragments shorter than    5863 bp
  20% of sequence in fragments shorter than    7126 bp
  25% of sequence in fragments shorter than    8374 bp
  30% of sequence in fragments shorter than    9626 bp
  35% of sequence in fragments shorter than   10921 bp
  40% of sequence in fragments shorter than   12276 bp
  45% of sequence in fragments shorter than   13711 bp
  50% of sequence in fragments shorter than   15210 bp
  55% of sequence in fragments shorter than   16864 bp
  60% of sequence in fragments shorter than   18689 bp
  65% of sequence in fragments shorter than   20698 bp
  70% of sequence in fragments shorter than   22992 bp
  75% of sequence in fragments shorter than   25817 bp
  80% of sequence in fragments shorter than   29256 bp
  85% of sequence in fragments shorter than   33723 bp
  90% of sequence in fragments shorter than   40490 bp
  95% of sequence in fragments shorter than   54488 bp

# BstEII.PvuI (GGTNACC+CGATCG) double digest, 349 thousand fragments (335865+14186 sites), 8520 bp average fragment length
   5% of sequence in fragments shorter than    2952 bp
  10% of sequence in fragments shorter than    4524 bp
  15% of sequence in fragments shorter than    5874 bp
  20% of sequence in fragments shorter than    7143 bp
  25% of sequence in fragments shorter than    8403 bp
  30% of sequence in fragments shorter than    9661 bp
  35% of sequence in fragments shorter than   10958 bp
  40% of sequence in fragments shorter than   12314 bp
  45% of sequence in fragments shorter than   13753 bp
  50% of sequence in fragments shorter than   15274 bp
  55% of sequence in fragments shorter than   16935 bp
  60% of sequence in fragments shorter than   18754 bp
  65% of sequence in fragments shorter than   20765 bp
  70% of sequence in fragments shorter than   23058 bp
  75% of sequence in fragments shorter than   25880 bp
  80% of sequence in fragments shorter than   29283 bp
  85% of sequence in fragments shorter than   33763 bp
  90% of sequence in fragments shorter than   40296 bp
  95% of sequence in fragments shorter than   53738 bp

# BstEII.FseI (GGTNACC+GGCCGGCC) double digest, 349 thousand fragments (335865+13662 sites), 8532 bp average fragment length
   5% of sequence in fragments shorter than    2962 bp
  10% of sequence in fragments shorter than    4538 bp
  15% of sequence in fragments shorter than    5902 bp
  20% of sequence in fragments shorter than    7184 bp
  25% of sequence in fragments shorter than    8448 bp
  30% of sequence in fragments shorter than    9728 bp
  35% of sequence in fragments shorter than   11044 bp
  40% of sequence in fragments shorter than   12430 bp
  45% of sequence in fragments shorter than   13893 bp
  50% of sequence in fragments shorter than   15421 bp
  55% of sequence in fragments shorter than   17096 bp
  60% of sequence in fragments shorter than   18926 bp
  65% of sequence in fragments shorter than   20985 bp
  70% of sequence in fragments shorter than   23328 bp
  75% of sequence in fragments shorter than   26221 bp
  80% of sequence in fragments shorter than   29722 bp
  85% of sequence in fragments shorter than   34329 bp
  90% of sequence in fragments shorter than   41196 bp
  95% of sequence in fragments shorter than   55780 bp

# SwaI.XhoI (ATTTAAAT+CTCGAG) double digest, 347 thousand fragments (226602+121308 sites), 8572 bp average fragment length
   5% of sequence in fragments shorter than    3050 bp
  10% of sequence in fragments shorter than    4610 bp
  15% of sequence in fragments shorter than    5948 bp
  20% of sequence in fragments shorter than    7211 bp
  25% of sequence in fragments shorter than    8440 bp
  30% of sequence in fragments shorter than    9658 bp
  35% of sequence in fragments shorter than   10886 bp
  40% of sequence in fragments shorter than   12185 bp
  45% of sequence in fragments shorter than   13536 bp
  50% of sequence in fragments shorter than   14973 bp
  55% of sequence in fragments shorter than   16527 bp
  60% of sequence in fragments shorter than   18178 bp
  65% of sequence in fragments shorter than   20046 bp
  70% of sequence in fragments shorter than   22189 bp
  75% of sequence in fragments shorter than   24750 bp
  80% of sequence in fragments shorter than   27898 bp
  85% of sequence in fragments shorter than   32011 bp
  90% of sequence in fragments shorter than   38151 bp
  95% of sequence in fragments shorter than   50913 bp

# KpnI.PspXI (GGTACC+VCTCGAGB) double digest, 346 thousand fragments (287974+59193 sites), 8591 bp average fragment length
   5% of sequence in fragments shorter than    2818 bp
  10% of sequence in fragments shorter than    4444 bp
  15% of sequence in fragments shorter than    5894 bp
  20% of sequence in fragments shorter than    7238 bp
  25% of sequence in fragments shorter than    8537 bp
  30% of sequence in fragments shorter than    9836 bp
  35% of sequence in fragments shorter than   11179 bp
  40% of sequence in fragments shorter than   12550 bp
  45% of sequence in fragments shorter than   13986 bp
  50% of sequence in fragments shorter than   15494 bp
  55% of sequence in fragments shorter than   17128 bp
  60% of sequence in fragments shorter than   18915 bp
  65% of sequence in fragments shorter than   20892 bp
  70% of sequence in fragments shorter than   23127 bp
  75% of sequence in fragments shorter than   25772 bp
  80% of sequence in fragments shorter than   29057 bp
  85% of sequence in fragments shorter than   33355 bp
  90% of sequence in fragments shorter than   39829 bp
  95% of sequence in fragments shorter than   52990 bp

# NheI.PspXI (GCTAGC+VCTCGAGB) double digest, 346 thousand fragments (287384+59193 sites), 8607 bp average fragment length
   5% of sequence in fragments shorter than    2767 bp
  10% of sequence in fragments shorter than    4489 bp
  15% of sequence in fragments shorter than    5980 bp
  20% of sequence in fragments shorter than    7353 bp
  25% of sequence in fragments shorter than    8673 bp
  30% of sequence in fragments shorter than   10014 bp
  35% of sequence in fragments shorter than   11348 bp
  40% of sequence in fragments shorter than   12721 bp
  45% of sequence in fragments shorter than   14193 bp
  50% of sequence in fragments shorter than   15735 bp
  55% of sequence in fragments shorter than   17400 bp
  60% of sequence in fragments shorter than   19217 bp
  65% of sequence in fragments shorter than   21220 bp
  70% of sequence in fragments shorter than   23574 bp
  75% of sequence in fragments shorter than   26194 bp
  80% of sequence in fragments shorter than   29444 bp
  85% of sequence in fragments shorter than   33747 bp
  90% of sequence in fragments shorter than   39778 bp
  95% of sequence in fragments shorter than   52323 bp

# BstEII.NotI (GGTNACC+GCGGCCGC) double digest, 345 thousand fragments (335865+9795 sites), 8627 bp average fragment length
   5% of sequence in fragments shorter than    3007 bp
  10% of sequence in fragments shorter than    4602 bp
  15% of sequence in fragments shorter than    5980 bp
  20% of sequence in fragments shorter than    7275 bp
  25% of sequence in fragments shorter than    8548 bp
  30% of sequence in fragments shorter than    9844 bp
  35% of sequence in fragments shorter than   11174 bp
  40% of sequence in fragments shorter than   12558 bp
  45% of sequence in fragments shorter than   14033 bp
  50% of sequence in fragments shorter than   15565 bp
  55% of sequence in fragments shorter than   17274 bp
  60% of sequence in fragments shorter than   19137 bp
  65% of sequence in fragments shorter than   21198 bp
  70% of sequence in fragments shorter than   23567 bp
  75% of sequence in fragments shorter than   26471 bp
  80% of sequence in fragments shorter than   30006 bp
  85% of sequence in fragments shorter than   34577 bp
  90% of sequence in fragments shorter than   41486 bp
  95% of sequence in fragments shorter than   56212 bp

# BstEII.RsrII (GGTNACC+CGGWCCG) double digest, 345 thousand fragments (335865+9764 sites), 8628 bp average fragment length
   5% of sequence in fragments shorter than    3001 bp
  10% of sequence in fragments shorter than    4594 bp
  15% of sequence in fragments shorter than    5962 bp
  20% of sequence in fragments shorter than    7252 bp
  25% of sequence in fragments shorter than    8522 bp
  30% of sequence in fragments shorter than    9807 bp
  35% of sequence in fragments shorter than   11137 bp
  40% of sequence in fragments shorter than   12512 bp
  45% of sequence in fragments shorter than   13987 bp
  50% of sequence in fragments shorter than   15522 bp
  55% of sequence in fragments shorter than   17221 bp
  60% of sequence in fragments shorter than   19059 bp
  65% of sequence in fragments shorter than   21103 bp
  70% of sequence in fragments shorter than   23444 bp
  75% of sequence in fragments shorter than   26368 bp
  80% of sequence in fragments shorter than   29875 bp
  85% of sequence in fragments shorter than   34418 bp
  90% of sequence in fragments shorter than   41349 bp
  95% of sequence in fragments shorter than   55862 bp

# AgeI.KpnI (ACCGGT+GGTACC) double digest, 341 thousand fragments (53386+287974 sites), 8737 bp average fragment length
   5% of sequence in fragments shorter than    2907 bp
  10% of sequence in fragments shorter than    4620 bp
  15% of sequence in fragments shorter than    6095 bp
  20% of sequence in fragments shorter than    7472 bp
  25% of sequence in fragments shorter than    8804 bp
  30% of sequence in fragments shorter than   10126 bp
  35% of sequence in fragments shorter than   11487 bp
  40% of sequence in fragments shorter than   12911 bp
  45% of sequence in fragments shorter than   14373 bp
  50% of sequence in fragments shorter than   15897 bp
  55% of sequence in fragments shorter than   17551 bp
  60% of sequence in fragments shorter than   19392 bp
  65% of sequence in fragments shorter than   21401 bp
  70% of sequence in fragments shorter than   23709 bp
  75% of sequence in fragments shorter than   26422 bp
  80% of sequence in fragments shorter than   29778 bp
  85% of sequence in fragments shorter than   34119 bp
  90% of sequence in fragments shorter than   40619 bp
  95% of sequence in fragments shorter than   53615 bp

# AgeI.NheI (ACCGGT+GCTAGC) double digest, 340 thousand fragments (53386+287384 sites), 8753 bp average fragment length
   5% of sequence in fragments shorter than    2802 bp
  10% of sequence in fragments shorter than    4602 bp
  15% of sequence in fragments shorter than    6148 bp
  20% of sequence in fragments shorter than    7566 bp
  25% of sequence in fragments shorter than    8939 bp
  30% of sequence in fragments shorter than   10284 bp
  35% of sequence in fragments shorter than   11659 bp
  40% of sequence in fragments shorter than   13072 bp
  45% of sequence in fragments shorter than   14566 bp
  50% of sequence in fragments shorter than   16141 bp
  55% of sequence in fragments shorter than   17840 bp
  60% of sequence in fragments shorter than   19684 bp
  65% of sequence in fragments shorter than   21711 bp
  70% of sequence in fragments shorter than   24032 bp
  75% of sequence in fragments shorter than   26698 bp
  80% of sequence in fragments shorter than   30014 bp
  85% of sequence in fragments shorter than   34319 bp
  90% of sequence in fragments shorter than   40651 bp
  95% of sequence in fragments shorter than   52926 bp

# AscI.BstEII (GGCGCGCC+GGTNACC) double digest, 340 thousand fragments (4751+335865 sites), 8755 bp average fragment length
   5% of sequence in fragments shorter than    3047 bp
  10% of sequence in fragments shorter than    4658 bp
  15% of sequence in fragments shorter than    6047 bp
  20% of sequence in fragments shorter than    7350 bp
  25% of sequence in fragments shorter than    8629 bp
  30% of sequence in fragments shorter than    9940 bp
  35% of sequence in fragments shorter than   11269 bp
  40% of sequence in fragments shorter than   12660 bp
  45% of sequence in fragments shorter than   14147 bp
  50% of sequence in fragments shorter than   15690 bp
  55% of sequence in fragments shorter than   17392 bp
  60% of sequence in fragments shorter than   19266 bp
  65% of sequence in fragments shorter than   21330 bp
  70% of sequence in fragments shorter than   23717 bp
  75% of sequence in fragments shorter than   26638 bp
  80% of sequence in fragments shorter than   30176 bp
  85% of sequence in fragments shorter than   34775 bp
  90% of sequence in fragments shorter than   41630 bp
  95% of sequence in fragments shorter than   56369 bp

# PmeI.SexAI (GTTTAAAC+ACCWGGT) double digest, 339 thousand fragments (42094+297900 sites), 8773 bp average fragment length
   5% of sequence in fragments shorter than    3054 bp
  10% of sequence in fragments shorter than    4644 bp
  15% of sequence in fragments shorter than    6042 bp
  20% of sequence in fragments shorter than    7336 bp
  25% of sequence in fragments shorter than    8593 bp
  30% of sequence in fragments shorter than    9854 bp
  35% of sequence in fragments shorter than   11154 bp
  40% of sequence in fragments shorter than   12488 bp
  45% of sequence in fragments shorter than   13862 bp
  50% of sequence in fragments shorter than   15350 bp
  55% of sequence in fragments shorter than   16921 bp
  60% of sequence in fragments shorter than   18679 bp
  65% of sequence in fragments shorter than   20583 bp
  70% of sequence in fragments shorter than   22778 bp
  75% of sequence in fragments shorter than   25369 bp
  80% of sequence in fragments shorter than   28522 bp
  85% of sequence in fragments shorter than   32638 bp
  90% of sequence in fragments shorter than   38846 bp
  95% of sequence in fragments shorter than   51197 bp

# AclI.PacI (AACGTT+TTAATTAA) double digest, 331 thousand fragments (172706+159455 sites), 8982 bp average fragment length
   5% of sequence in fragments shorter than    3162 bp
  10% of sequence in fragments shorter than    4852 bp
  15% of sequence in fragments shorter than    6324 bp
  20% of sequence in fragments shorter than    7728 bp
  25% of sequence in fragments shorter than    9095 bp
  30% of sequence in fragments shorter than   10463 bp
  35% of sequence in fragments shorter than   11854 bp
  40% of sequence in fragments shorter than   13304 bp
  45% of sequence in fragments shorter than   14847 bp
  50% of sequence in fragments shorter than   16478 bp
  55% of sequence in fragments shorter than   18202 bp
  60% of sequence in fragments shorter than   20122 bp
  65% of sequence in fragments shorter than   22332 bp
  70% of sequence in fragments shorter than   24772 bp
  75% of sequence in fragments shorter than   27669 bp
  80% of sequence in fragments shorter than   31258 bp
  85% of sequence in fragments shorter than   35961 bp
  90% of sequence in fragments shorter than   42962 bp
  95% of sequence in fragments shorter than   57106 bp

# AclI.Hpy99I (AACGTT+CGWCG) double digest, 330 thousand fragments (172706+157917 sites), 9024 bp average fragment length
   5% of sequence in fragments shorter than    3191 bp
  10% of sequence in fragments shorter than    5069 bp
  15% of sequence in fragments shorter than    6779 bp
  20% of sequence in fragments shorter than    8414 bp
  25% of sequence in fragments shorter than    9968 bp
  30% of sequence in fragments shorter than   11545 bp
  35% of sequence in fragments shorter than   13161 bp
  40% of sequence in fragments shorter than   14839 bp
  45% of sequence in fragments shorter than   16595 bp
  50% of sequence in fragments shorter than   18482 bp
  55% of sequence in fragments shorter than   20515 bp
  60% of sequence in fragments shorter than   22686 bp
  65% of sequence in fragments shorter than   25078 bp
  70% of sequence in fragments shorter than   27856 bp
  75% of sequence in fragments shorter than   31099 bp
  80% of sequence in fragments shorter than   35023 bp
  85% of sequence in fragments shorter than   39961 bp
  90% of sequence in fragments shorter than   47045 bp
  95% of sequence in fragments shorter than   60020 bp

# KpnI.PmeI (GGTACC+GTTTAAAC) double digest, 329 thousand fragments (287974+42094 sites), 9037 bp average fragment length
   5% of sequence in fragments shorter than    2984 bp
  10% of sequence in fragments shorter than    4710 bp
  15% of sequence in fragments shorter than    6193 bp
  20% of sequence in fragments shorter than    7588 bp
  25% of sequence in fragments shorter than    8941 bp
  30% of sequence in fragments shorter than   10277 bp
  35% of sequence in fragments shorter than   11636 bp
  40% of sequence in fragments shorter than   13029 bp
  45% of sequence in fragments shorter than   14491 bp
  50% of sequence in fragments shorter than   16031 bp
  55% of sequence in fragments shorter than   17681 bp
  60% of sequence in fragments shorter than   19516 bp
  65% of sequence in fragments shorter than   21536 bp
  70% of sequence in fragments shorter than   23823 bp
  75% of sequence in fragments shorter than   26480 bp
  80% of sequence in fragments shorter than   29751 bp
  85% of sequence in fragments shorter than   34061 bp
  90% of sequence in fragments shorter than   40272 bp
  95% of sequence in fragments shorter than   53017 bp

# NheI.PmeI (GCTAGC+GTTTAAAC) double digest, 329 thousand fragments (287384+42094 sites), 9054 bp average fragment length
   5% of sequence in fragments shorter than    2883 bp
  10% of sequence in fragments shorter than    4720 bp
  15% of sequence in fragments shorter than    6292 bp
  20% of sequence in fragments shorter than    7734 bp
  25% of sequence in fragments shorter than    9120 bp
  30% of sequence in fragments shorter than   10501 bp
  35% of sequence in fragments shorter than   11891 bp
  40% of sequence in fragments shorter than   13329 bp
  45% of sequence in fragments shorter than   14824 bp
  50% of sequence in fragments shorter than   16456 bp
  55% of sequence in fragments shorter than   18177 bp
  60% of sequence in fragments shorter than   20058 bp
  65% of sequence in fragments shorter than   22129 bp
  70% of sequence in fragments shorter than   24571 bp
  75% of sequence in fragments shorter than   27350 bp
  80% of sequence in fragments shorter than   30765 bp
  85% of sequence in fragments shorter than   35138 bp
  90% of sequence in fragments shorter than   41769 bp
  95% of sequence in fragments shorter than   54524 bp

# SalI.SexAI (GTCGAC+ACCWGGT) double digest, 328 thousand fragments (31396+297900 sites), 9058 bp average fragment length
   5% of sequence in fragments shorter than    3128 bp
  10% of sequence in fragments shorter than    4787 bp
  15% of sequence in fragments shorter than    6237 bp
  20% of sequence in fragments shorter than    7600 bp
  25% of sequence in fragments shorter than    8904 bp
  30% of sequence in fragments shorter than   10242 bp
  35% of sequence in fragments shorter than   11607 bp
  40% of sequence in fragments shorter than   13011 bp
  45% of sequence in fragments shorter than   14504 bp
  50% of sequence in fragments shorter than   16063 bp
  55% of sequence in fragments shorter than   17765 bp
  60% of sequence in fragments shorter than   19607 bp
  65% of sequence in fragments shorter than   21643 bp
  70% of sequence in fragments shorter than   24010 bp
  75% of sequence in fragments shorter than   26758 bp
  80% of sequence in fragments shorter than   30218 bp
  85% of sequence in fragments shorter than   34822 bp
  90% of sequence in fragments shorter than   41527 bp
  95% of sequence in fragments shorter than   55458 bp

# BsrBI.ClaI (CCGCTC+ATCGAT) double digest, 321 thousand fragments (235523+86477 sites), 9266 bp average fragment length
   5% of sequence in fragments shorter than    3189 bp
  10% of sequence in fragments shorter than    5205 bp
  15% of sequence in fragments shorter than    6999 bp
  20% of sequence in fragments shorter than    8720 bp
  25% of sequence in fragments shorter than   10473 bp
  30% of sequence in fragments shorter than   12229 bp
  35% of sequence in fragments shorter than   14046 bp
  40% of sequence in fragments shorter than   15968 bp
  45% of sequence in fragments shorter than   17969 bp
  50% of sequence in fragments shorter than   20142 bp
  55% of sequence in fragments shorter than   22430 bp
  60% of sequence in fragments shorter than   25043 bp
  65% of sequence in fragments shorter than   27978 bp
  70% of sequence in fragments shorter than   31310 bp
  75% of sequence in fragments shorter than   35211 bp
  80% of sequence in fragments shorter than   40025 bp
  85% of sequence in fragments shorter than   46087 bp
  90% of sequence in fragments shorter than   54936 bp
  95% of sequence in fragments shorter than   72245 bp

# KpnI.SalI (GGTACC+GTCGAC) double digest, 319 thousand fragments (287974+31396 sites), 9340 bp average fragment length
   5% of sequence in fragments shorter than    3071 bp
  10% of sequence in fragments shorter than    4873 bp
  15% of sequence in fragments shorter than    6451 bp
  20% of sequence in fragments shorter than    7907 bp
  25% of sequence in fragments shorter than    9324 bp
  30% of sequence in fragments shorter than   10731 bp
  35% of sequence in fragments shorter than   12175 bp
  40% of sequence in fragments shorter than   13669 bp
  45% of sequence in fragments shorter than   15188 bp
  50% of sequence in fragments shorter than   16800 bp
  55% of sequence in fragments shorter than   18542 bp
  60% of sequence in fragments shorter than   20436 bp
  65% of sequence in fragments shorter than   22523 bp
  70% of sequence in fragments shorter than   24988 bp
  75% of sequence in fragments shorter than   27809 bp
  80% of sequence in fragments shorter than   31317 bp
  85% of sequence in fragments shorter than   35902 bp
  90% of sequence in fragments shorter than   42572 bp
  95% of sequence in fragments shorter than   55877 bp

# MluI.SexAI (ACGCGT+ACCWGGT) double digest, 319 thousand fragments (21415+297900 sites), 9341 bp average fragment length
   5% of sequence in fragments shorter than    3223 bp
  10% of sequence in fragments shorter than    4926 bp
  15% of sequence in fragments shorter than    6423 bp
  20% of sequence in fragments shorter than    7824 bp
  25% of sequence in fragments shorter than    9199 bp
  30% of sequence in fragments shorter than   10590 bp
  35% of sequence in fragments shorter than   12009 bp
  40% of sequence in fragments shorter than   13478 bp
  45% of sequence in fragments shorter than   15020 bp
  50% of sequence in fragments shorter than   16647 bp
  55% of sequence in fragments shorter than   18433 bp
  60% of sequence in fragments shorter than   20365 bp
  65% of sequence in fragments shorter than   22539 bp
  70% of sequence in fragments shorter than   25038 bp
  75% of sequence in fragments shorter than   27956 bp
  80% of sequence in fragments shorter than   31594 bp
  85% of sequence in fragments shorter than   36441 bp
  90% of sequence in fragments shorter than   43448 bp
  95% of sequence in fragments shorter than   59166 bp

# NheI.SalI (GCTAGC+GTCGAC) double digest, 318 thousand fragments (287384+31396 sites), 9358 bp average fragment length
   5% of sequence in fragments shorter than    2982 bp
  10% of sequence in fragments shorter than    4919 bp
  15% of sequence in fragments shorter than    6564 bp
  20% of sequence in fragments shorter than    8059 bp
  25% of sequence in fragments shorter than    9518 bp
  30% of sequence in fragments shorter than   10964 bp
  35% of sequence in fragments shorter than   12406 bp
  40% of sequence in fragments shorter than   13943 bp
  45% of sequence in fragments shorter than   15525 bp
  50% of sequence in fragments shorter than   17224 bp
  55% of sequence in fragments shorter than   19016 bp
  60% of sequence in fragments shorter than   20960 bp
  65% of sequence in fragments shorter than   23158 bp
  70% of sequence in fragments shorter than   25588 bp
  75% of sequence in fragments shorter than   28490 bp
  80% of sequence in fragments shorter than   32036 bp
  85% of sequence in fragments shorter than   36719 bp
  90% of sequence in fragments shorter than   43416 bp
  95% of sequence in fragments shorter than   56359 bp

# Hpy99I.PacI (CGWCG+TTAATTAA) double digest, 317 thousand fragments (157917+159455 sites), 9400 bp average fragment length
   5% of sequence in fragments shorter than    3563 bp
  10% of sequence in fragments shorter than    5564 bp
  15% of sequence in fragments shorter than    7291 bp
  20% of sequence in fragments shorter than    8918 bp
  25% of sequence in fragments shorter than   10497 bp
  30% of sequence in fragments shorter than   12084 bp
  35% of sequence in fragments shorter than   13710 bp
  40% of sequence in fragments shorter than   15389 bp
  45% of sequence in fragments shorter than   17176 bp
  50% of sequence in fragments shorter than   19087 bp
  55% of sequence in fragments shorter than   21111 bp
  60% of sequence in fragments shorter than   23369 bp
  65% of sequence in fragments shorter than   25847 bp
  70% of sequence in fragments shorter than   28628 bp
  75% of sequence in fragments shorter than   31840 bp
  80% of sequence in fragments shorter than   35842 bp
  85% of sequence in fragments shorter than   40947 bp
  90% of sequence in fragments shorter than   48623 bp
  95% of sequence in fragments shorter than   63861 bp

# ClaI.KasI (ATCGAT+GGCGCC) double digest, 316 thousand fragments (86477+230381 sites), 9414 bp average fragment length
   5% of sequence in fragments shorter than    3245 bp
  10% of sequence in fragments shorter than    5141 bp
  15% of sequence in fragments shorter than    6849 bp
  20% of sequence in fragments shorter than    8506 bp
  25% of sequence in fragments shorter than   10167 bp
  30% of sequence in fragments shorter than   11857 bp
  35% of sequence in fragments shorter than   13640 bp
  40% of sequence in fragments shorter than   15546 bp
  45% of sequence in fragments shorter than   17527 bp
  50% of sequence in fragments shorter than   19696 bp
  55% of sequence in fragments shorter than   22032 bp
  60% of sequence in fragments shorter than   24668 bp
  65% of sequence in fragments shorter than   27579 bp
  70% of sequence in fragments shorter than   31036 bp
  75% of sequence in fragments shorter than   35042 bp
  80% of sequence in fragments shorter than   39886 bp
  85% of sequence in fragments shorter than   46436 bp
  90% of sequence in fragments shorter than   56104 bp
  95% of sequence in fragments shorter than   76220 bp

# SexAI.SgrAI (ACCWGGT+CRCCGGYG) double digest, 312 thousand fragments (297900+15270 sites), 9524 bp average fragment length
   5% of sequence in fragments shorter than    3288 bp
  10% of sequence in fragments shorter than    5030 bp
  15% of sequence in fragments shorter than    6573 bp
  20% of sequence in fragments shorter than    7991 bp
  25% of sequence in fragments shorter than    9399 bp
  30% of sequence in fragments shorter than   10816 bp
  35% of sequence in fragments shorter than   12281 bp
  40% of sequence in fragments shorter than   13756 bp
  45% of sequence in fragments shorter than   15319 bp
  50% of sequence in fragments shorter than   16978 bp
  55% of sequence in fragments shorter than   18803 bp
  60% of sequence in fragments shorter than   20755 bp
  65% of sequence in fragments shorter than   22973 bp
  70% of sequence in fragments shorter than   25488 bp
  75% of sequence in fragments shorter than   28488 bp
  80% of sequence in fragments shorter than   32157 bp
  85% of sequence in fragments shorter than   37016 bp
  90% of sequence in fragments shorter than   44440 bp
  95% of sequence in fragments shorter than   60179 bp

# ClaI.SwaI (ATCGAT+ATTTAAAT) double digest, 312 thousand fragments (86477+226602 sites), 9527 bp average fragment length
   5% of sequence in fragments shorter than    3281 bp
  10% of sequence in fragments shorter than    4998 bp
  15% of sequence in fragments shorter than    6513 bp
  20% of sequence in fragments shorter than    7976 bp
  25% of sequence in fragments shorter than    9390 bp
  30% of sequence in fragments shorter than   10814 bp
  35% of sequence in fragments shorter than   12288 bp
  40% of sequence in fragments shorter than   13835 bp
  45% of sequence in fragments shorter than   15489 bp
  50% of sequence in fragments shorter than   17303 bp
  55% of sequence in fragments shorter than   19247 bp
  60% of sequence in fragments shorter than   21440 bp
  65% of sequence in fragments shorter than   23962 bp
  70% of sequence in fragments shorter than   26837 bp
  75% of sequence in fragments shorter than   30429 bp
  80% of sequence in fragments shorter than   34864 bp
  85% of sequence in fragments shorter than   41162 bp
  90% of sequence in fragments shorter than   50869 bp
  95% of sequence in fragments shorter than   73150 bp

# NruI.SexAI (TCGCGA+ACCWGGT) double digest, 312 thousand fragments (14872+297900 sites), 9536 bp average fragment length
   5% of sequence in fragments shorter than    3299 bp
  10% of sequence in fragments shorter than    5033 bp
  15% of sequence in fragments shorter than    6570 bp
  20% of sequence in fragments shorter than    7978 bp
  25% of sequence in fragments shorter than    9371 bp
  30% of sequence in fragments shorter than   10765 bp
  35% of sequence in fragments shorter than   12221 bp
  40% of sequence in fragments shorter than   13698 bp
  45% of sequence in fragments shorter than   15248 bp
  50% of sequence in fragments shorter than   16884 bp
  55% of sequence in fragments shorter than   18670 bp
  60% of sequence in fragments shorter than   20591 bp
  65% of sequence in fragments shorter than   22797 bp
  70% of sequence in fragments shorter than   25285 bp
  75% of sequence in fragments shorter than   28234 bp
  80% of sequence in fragments shorter than   31859 bp
  85% of sequence in fragments shorter than   36695 bp
  90% of sequence in fragments shorter than   43918 bp
  95% of sequence in fragments shorter than   59320 bp

# PvuI.SexAI (CGATCG+ACCWGGT) double digest, 311 thousand fragments (14186+297900 sites), 9558 bp average fragment length
   5% of sequence in fragments shorter than    3291 bp
  10% of sequence in fragments shorter than    5038 bp
  15% of sequence in fragments shorter than    6585 bp
  20% of sequence in fragments shorter than    8006 bp
  25% of sequence in fragments shorter than    9406 bp
  30% of sequence in fragments shorter than   10808 bp
  35% of sequence in fragments shorter than   12271 bp
  40% of sequence in fragments shorter than   13752 bp
  45% of sequence in fragments shorter than   15325 bp
  50% of sequence in fragments shorter than   16973 bp
  55% of sequence in fragments shorter than   18794 bp
  60% of sequence in fragments shorter than   20733 bp
  65% of sequence in fragments shorter than   22932 bp
  70% of sequence in fragments shorter than   25454 bp
  75% of sequence in fragments shorter than   28406 bp
  80% of sequence in fragments shorter than   32019 bp
  85% of sequence in fragments shorter than   36779 bp
  90% of sequence in fragments shorter than   43871 bp
  95% of sequence in fragments shorter than   58472 bp

# FseI.SexAI (GGCCGGCC+ACCWGGT) double digest, 311 thousand fragments (13662+297900 sites), 9573 bp average fragment length
   5% of sequence in fragments shorter than    3298 bp
  10% of sequence in fragments shorter than    5048 bp
  15% of sequence in fragments shorter than    6602 bp
  20% of sequence in fragments shorter than    8025 bp
  25% of sequence in fragments shorter than    9440 bp
  30% of sequence in fragments shorter than   10862 bp
  35% of sequence in fragments shorter than   12340 bp
  40% of sequence in fragments shorter than   13837 bp
  45% of sequence in fragments shorter than   15420 bp
  50% of sequence in fragments shorter than   17085 bp
  55% of sequence in fragments shorter than   18914 bp
  60% of sequence in fragments shorter than   20897 bp
  65% of sequence in fragments shorter than   23133 bp
  70% of sequence in fragments shorter than   25688 bp
  75% of sequence in fragments shorter than   28696 bp
  80% of sequence in fragments shorter than   32422 bp
  85% of sequence in fragments shorter than   37321 bp
  90% of sequence in fragments shorter than   44698 bp
  95% of sequence in fragments shorter than   60739 bp

# KpnI.MluI (GGTACC+ACGCGT) double digest, 309 thousand fragments (287974+21415 sites), 9641 bp average fragment length
   5% of sequence in fragments shorter than    3179 bp
  10% of sequence in fragments shorter than    5040 bp
  15% of sequence in fragments shorter than    6661 bp
  20% of sequence in fragments shorter than    8171 bp
  25% of sequence in fragments shorter than    9639 bp
  30% of sequence in fragments shorter than   11104 bp
  35% of sequence in fragments shorter than   12598 bp
  40% of sequence in fragments shorter than   14119 bp
  45% of sequence in fragments shorter than   15695 bp
  50% of sequence in fragments shorter than   17376 bp
  55% of sequence in fragments shorter than   19207 bp
  60% of sequence in fragments shorter than   21189 bp
  65% of sequence in fragments shorter than   23374 bp
  70% of sequence in fragments shorter than   25907 bp
  75% of sequence in fragments shorter than   28887 bp
  80% of sequence in fragments shorter than   32524 bp
  85% of sequence in fragments shorter than   37355 bp
  90% of sequence in fragments shorter than   44438 bp
  95% of sequence in fragments shorter than   58585 bp

# MluI.NheI (ACGCGT+GCTAGC) double digest, 308 thousand fragments (21415+287384 sites), 9661 bp average fragment length
   5% of sequence in fragments shorter than    3085 bp
  10% of sequence in fragments shorter than    5096 bp
  15% of sequence in fragments shorter than    6801 bp
  20% of sequence in fragments shorter than    8352 bp
  25% of sequence in fragments shorter than    9863 bp
  30% of sequence in fragments shorter than   11347 bp
  35% of sequence in fragments shorter than   12852 bp
  40% of sequence in fragments shorter than   14407 bp
  45% of sequence in fragments shorter than   16044 bp
  50% of sequence in fragments shorter than   17783 bp
  55% of sequence in fragments shorter than   19646 bp
  60% of sequence in fragments shorter than   21670 bp
  65% of sequence in fragments shorter than   23954 bp
  70% of sequence in fragments shorter than   26487 bp
  75% of sequence in fragments shorter than   29489 bp
  80% of sequence in fragments shorter than   33179 bp
  85% of sequence in fragments shorter than   37933 bp
  90% of sequence in fragments shorter than   44804 bp
  95% of sequence in fragments shorter than   58355 bp

# NotI.SexAI (GCGGCCGC+ACCWGGT) double digest, 307 thousand fragments (9795+297900 sites), 9694 bp average fragment length
   5% of sequence in fragments shorter than    3355 bp
  10% of sequence in fragments shorter than    5130 bp
  15% of sequence in fragments shorter than    6705 bp
  20% of sequence in fragments shorter than    8147 bp
  25% of sequence in fragments shorter than    9576 bp
  30% of sequence in fragments shorter than   11018 bp
  35% of sequence in fragments shorter than   12497 bp
  40% of sequence in fragments shorter than   14000 bp
  45% of sequence in fragments shorter than   15594 bp
  50% of sequence in fragments shorter than   17280 bp
  55% of sequence in fragments shorter than   19112 bp
  60% of sequence in fragments shorter than   21116 bp
  65% of sequence in fragments shorter than   23349 bp
  70% of sequence in fragments shorter than   25916 bp
  75% of sequence in fragments shorter than   28976 bp
  80% of sequence in fragments shorter than   32715 bp
  85% of sequence in fragments shorter than   37641 bp
  90% of sequence in fragments shorter than   45035 bp
  95% of sequence in fragments shorter than   61147 bp

# RsrII.SexAI (CGGWCCG+ACCWGGT) double digest, 307 thousand fragments (9764+297900 sites), 9695 bp average fragment length
   5% of sequence in fragments shorter than    3349 bp
  10% of sequence in fragments shorter than    5114 bp
  15% of sequence in fragments shorter than    6690 bp
  20% of sequence in fragments shorter than    8124 bp
  25% of sequence in fragments shorter than    9551 bp
  30% of sequence in fragments shorter than   10988 bp
  35% of sequence in fragments shorter than   12465 bp
  40% of sequence in fragments shorter than   13970 bp
  45% of sequence in fragments shorter than   15563 bp
  50% of sequence in fragments shorter than   17247 bp
  55% of sequence in fragments shorter than   19075 bp
  60% of sequence in fragments shorter than   21065 bp
  65% of sequence in fragments shorter than   23303 bp
  70% of sequence in fragments shorter than   25839 bp
  75% of sequence in fragments shorter than   28882 bp
  80% of sequence in fragments shorter than   32618 bp
  85% of sequence in fragments shorter than   37481 bp
  90% of sequence in fragments shorter than   44832 bp
  95% of sequence in fragments shorter than   60815 bp

# AatII.BsrBI (GACGTC+CCGCTC) double digest, 306 thousand fragments (71089+235523 sites), 9732 bp average fragment length
   5% of sequence in fragments shorter than    3255 bp
  10% of sequence in fragments shorter than    5474 bp
  15% of sequence in fragments shorter than    7541 bp
  20% of sequence in fragments shorter than    9584 bp
  25% of sequence in fragments shorter than   11675 bp
  30% of sequence in fragments shorter than   13828 bp
  35% of sequence in fragments shorter than   16095 bp
  40% of sequence in fragments shorter than   18452 bp
  45% of sequence in fragments shorter than   21044 bp
  50% of sequence in fragments shorter than   23804 bp
  55% of sequence in fragments shorter than   26941 bp
  60% of sequence in fragments shorter than   30385 bp
  65% of sequence in fragments shorter than   34141 bp
  70% of sequence in fragments shorter than   38575 bp
  75% of sequence in fragments shorter than   43799 bp
  80% of sequence in fragments shorter than   50135 bp
  85% of sequence in fragments shorter than   58913 bp
  90% of sequence in fragments shorter than   70887 bp
  95% of sequence in fragments shorter than   94746 bp

# AclI.SnaBI (AACGTT+TACGTA) double digest, 304 thousand fragments (172706+132299 sites), 9783 bp average fragment length
   5% of sequence in fragments shorter than    3367 bp
  10% of sequence in fragments shorter than    5199 bp
  15% of sequence in fragments shorter than    6813 bp
  20% of sequence in fragments shorter than    8324 bp
  25% of sequence in fragments shorter than    9785 bp
  30% of sequence in fragments shorter than   11245 bp
  35% of sequence in fragments shorter than   12734 bp
  40% of sequence in fragments shorter than   14298 bp
  45% of sequence in fragments shorter than   15934 bp
  50% of sequence in fragments shorter than   17637 bp
  55% of sequence in fragments shorter than   19550 bp
  60% of sequence in fragments shorter than   21635 bp
  65% of sequence in fragments shorter than   23924 bp
  70% of sequence in fragments shorter than   26568 bp
  75% of sequence in fragments shorter than   29562 bp
  80% of sequence in fragments shorter than   33202 bp
  85% of sequence in fragments shorter than   38113 bp
  90% of sequence in fragments shorter than   45383 bp
  95% of sequence in fragments shorter than   58405 bp

# KpnI.SgrAI (GGTACC+CRCCGGYG) double digest, 302 thousand fragments (287974+15270 sites), 9836 bp average fragment length
   5% of sequence in fragments shorter than    3246 bp
  10% of sequence in fragments shorter than    5156 bp
  15% of sequence in fragments shorter than    6820 bp
  20% of sequence in fragments shorter than    8379 bp
  25% of sequence in fragments shorter than    9870 bp
  30% of sequence in fragments shorter than   11376 bp
  35% of sequence in fragments shorter than   12907 bp
  40% of sequence in fragments shorter than   14470 bp
  45% of sequence in fragments shorter than   16085 bp
  50% of sequence in fragments shorter than   17809 bp
  55% of sequence in fragments shorter than   19685 bp
  60% of sequence in fragments shorter than   21720 bp
  65% of sequence in fragments shorter than   23987 bp
  70% of sequence in fragments shorter than   26551 bp
  75% of sequence in fragments shorter than   29567 bp
  80% of sequence in fragments shorter than   33315 bp
  85% of sequence in fragments shorter than   38201 bp
  90% of sequence in fragments shorter than   45459 bp
  95% of sequence in fragments shorter than   60160 bp

# KpnI.NruI (GGTACC+TCGCGA) double digest, 302 thousand fragments (287974+14872 sites), 9849 bp average fragment length
   5% of sequence in fragments shorter than    3253 bp
  10% of sequence in fragments shorter than    5151 bp
  15% of sequence in fragments shorter than    6810 bp
  20% of sequence in fragments shorter than    8354 bp
  25% of sequence in fragments shorter than    9842 bp
  30% of sequence in fragments shorter than   11329 bp
  35% of sequence in fragments shorter than   12860 bp
  40% of sequence in fragments shorter than   14413 bp
  45% of sequence in fragments shorter than   16012 bp
  50% of sequence in fragments shorter than   17719 bp
  55% of sequence in fragments shorter than   19570 bp
  60% of sequence in fragments shorter than   21583 bp
  65% of sequence in fragments shorter than   23845 bp
  70% of sequence in fragments shorter than   26378 bp
  75% of sequence in fragments shorter than   29407 bp
  80% of sequence in fragments shorter than   33095 bp
  85% of sequence in fragments shorter than   37978 bp
  90% of sequence in fragments shorter than   45139 bp
  95% of sequence in fragments shorter than   59594 bp

# AscI.SexAI (GGCGCGCC+ACCWGGT) double digest, 302 thousand fragments (4751+297900 sites), 9855 bp average fragment length
   5% of sequence in fragments shorter than    3407 bp
  10% of sequence in fragments shorter than    5206 bp
  15% of sequence in fragments shorter than    6795 bp
  20% of sequence in fragments shorter than    8252 bp
  25% of sequence in fragments shorter than    9692 bp
  30% of sequence in fragments shorter than   11153 bp
  35% of sequence in fragments shorter than   12638 bp
  40% of sequence in fragments shorter than   14169 bp
  45% of sequence in fragments shorter than   15771 bp
  50% of sequence in fragments shorter than   17476 bp
  55% of sequence in fragments shorter than   19337 bp
  60% of sequence in fragments shorter than   21335 bp
  65% of sequence in fragments shorter than   23589 bp
  70% of sequence in fragments shorter than   26169 bp
  75% of sequence in fragments shorter than   29254 bp
  80% of sequence in fragments shorter than   33041 bp
  85% of sequence in fragments shorter than   37957 bp
  90% of sequence in fragments shorter than   45437 bp
  95% of sequence in fragments shorter than   61584 bp

# NheI.SgrAI (GCTAGC+CRCCGGYG) double digest, 302 thousand fragments (287384+15270 sites), 9856 bp average fragment length
   5% of sequence in fragments shorter than    3134 bp
  10% of sequence in fragments shorter than    5210 bp
  15% of sequence in fragments shorter than    6965 bp
  20% of sequence in fragments shorter than    8563 bp
  25% of sequence in fragments shorter than   10112 bp
  30% of sequence in fragments shorter than   11640 bp
  35% of sequence in fragments shorter than   13192 bp
  40% of sequence in fragments shorter than   14785 bp
  45% of sequence in fragments shorter than   16473 bp
  50% of sequence in fragments shorter than   18270 bp
  55% of sequence in fragments shorter than   20171 bp
  60% of sequence in fragments shorter than   22281 bp
  65% of sequence in fragments shorter than   24605 bp
  70% of sequence in fragments shorter than   27251 bp
  75% of sequence in fragments shorter than   30320 bp
  80% of sequence in fragments shorter than   34058 bp
  85% of sequence in fragments shorter than   39035 bp
  90% of sequence in fragments shorter than   46236 bp
  95% of sequence in fragments shorter than   60347 bp

# BsrBI.SacII (CCGCTC+CCGCGG) double digest, 302 thousand fragments (235523+66901 sites), 9867 bp average fragment length
   5% of sequence in fragments shorter than    3643 bp
  10% of sequence in fragments shorter than    6376 bp
  15% of sequence in fragments shorter than    8954 bp
  20% of sequence in fragments shorter than   11560 bp
  25% of sequence in fragments shorter than   14231 bp
  30% of sequence in fragments shorter than   17022 bp
  35% of sequence in fragments shorter than   20006 bp
  40% of sequence in fragments shorter than   23108 bp
  45% of sequence in fragments shorter than   26581 bp
  50% of sequence in fragments shorter than   30339 bp
  55% of sequence in fragments shorter than   34434 bp
  60% of sequence in fragments shorter than   39010 bp
  65% of sequence in fragments shorter than   44221 bp
  70% of sequence in fragments shorter than   50140 bp
  75% of sequence in fragments shorter than   57263 bp
  80% of sequence in fragments shorter than   66031 bp
  85% of sequence in fragments shorter than   77629 bp
  90% of sequence in fragments shorter than   95361 bp
  95% of sequence in fragments shorter than  128912 bp

# NheI.NruI (GCTAGC+TCGCGA) double digest, 301 thousand fragments (287384+14872 sites), 9869 bp average fragment length
   5% of sequence in fragments shorter than    3140 bp
  10% of sequence in fragments shorter than    5210 bp
  15% of sequence in fragments shorter than    6954 bp
  20% of sequence in fragments shorter than    8545 bp
  25% of sequence in fragments shorter than   10089 bp
  30% of sequence in fragments shorter than   11607 bp
  35% of sequence in fragments shorter than   13154 bp
  40% of sequence in fragments shorter than   14747 bp
  45% of sequence in fragments shorter than   16431 bp
  50% of sequence in fragments shorter than   18218 bp
  55% of sequence in fragments shorter than   20117 bp
  60% of sequence in fragments shorter than   22186 bp
  65% of sequence in fragments shorter than   24527 bp
  70% of sequence in fragments shorter than   27131 bp
  75% of sequence in fragments shorter than   30200 bp
  80% of sequence in fragments shorter than   33926 bp
  85% of sequence in fragments shorter than   38735 bp
  90% of sequence in fragments shorter than   45648 bp
  95% of sequence in fragments shorter than   59670 bp

# KpnI.PvuI (GGTACC+CGATCG) double digest, 301 thousand fragments (287974+14186 sites), 9872 bp average fragment length
   5% of sequence in fragments shorter than    3238 bp
  10% of sequence in fragments shorter than    5163 bp
  15% of sequence in fragments shorter than    6836 bp
  20% of sequence in fragments shorter than    8390 bp
  25% of sequence in fragments shorter than    9882 bp
  30% of sequence in fragments shorter than   11390 bp
  35% of sequence in fragments shorter than   12915 bp
  40% of sequence in fragments shorter than   14475 bp
  45% of sequence in fragments shorter than   16089 bp
  50% of sequence in fragments shorter than   17804 bp
  55% of sequence in fragments shorter than   19678 bp
  60% of sequence in fragments shorter than   21714 bp
  65% of sequence in fragments shorter than   23960 bp
  70% of sequence in fragments shorter than   26538 bp
  75% of sequence in fragments shorter than   29558 bp
  80% of sequence in fragments shorter than   33245 bp
  85% of sequence in fragments shorter than   38091 bp
  90% of sequence in fragments shorter than   45200 bp
  95% of sequence in fragments shorter than   58970 bp

# FseI.KpnI (GGCCGGCC+GGTACC) double digest, 301 thousand fragments (13662+287974 sites), 9888 bp average fragment length
   5% of sequence in fragments shorter than    3262 bp
  10% of sequence in fragments shorter than    5175 bp
  15% of sequence in fragments shorter than    6851 bp
  20% of sequence in fragments shorter than    8411 bp
  25% of sequence in fragments shorter than    9903 bp
  30% of sequence in fragments shorter than   11414 bp
  35% of sequence in fragments shorter than   12959 bp
  40% of sequence in fragments shorter than   14537 bp
  45% of sequence in fragments shorter than   16165 bp
  50% of sequence in fragments shorter than   17920 bp
  55% of sequence in fragments shorter than   19803 bp
  60% of sequence in fragments shorter than   21839 bp
  65% of sequence in fragments shorter than   24129 bp
  70% of sequence in fragments shorter than   26743 bp
  75% of sequence in fragments shorter than   29810 bp
  80% of sequence in fragments shorter than   33579 bp
  85% of sequence in fragments shorter than   38542 bp
  90% of sequence in fragments shorter than   45752 bp
  95% of sequence in fragments shorter than   60431 bp

# NheI.PvuI (GCTAGC+CGATCG) double digest, 301 thousand fragments (287384+14186 sites), 9892 bp average fragment length
   5% of sequence in fragments shorter than    3126 bp
  10% of sequence in fragments shorter than    5215 bp
  15% of sequence in fragments shorter than    6982 bp
  20% of sequence in fragments shorter than    8585 bp
  25% of sequence in fragments shorter than   10142 bp
  30% of sequence in fragments shorter than   11663 bp
  35% of sequence in fragments shorter than   13207 bp
  40% of sequence in fragments shorter than   14807 bp
  45% of sequence in fragments shorter than   16514 bp
  50% of sequence in fragments shorter than   18315 bp
  55% of sequence in fragments shorter than   20221 bp
  60% of sequence in fragments shorter than   22321 bp
  65% of sequence in fragments shorter than   24647 bp
  70% of sequence in fragments shorter than   27259 bp
  75% of sequence in fragments shorter than   30306 bp
  80% of sequence in fragments shorter than   34057 bp
  85% of sequence in fragments shorter than   38922 bp
  90% of sequence in fragments shorter than   45734 bp
  95% of sequence in fragments shorter than   59335 bp

# AatII.KasI (GACGTC+GGCGCC) double digest, 301 thousand fragments (71089+230381 sites), 9895 bp average fragment length
   5% of sequence in fragments shorter than    3317 bp
  10% of sequence in fragments shorter than    5391 bp
  15% of sequence in fragments shorter than    7307 bp
  20% of sequence in fragments shorter than    9243 bp
  25% of sequence in fragments shorter than   11196 bp
  30% of sequence in fragments shorter than   13251 bp
  35% of sequence in fragments shorter than   15459 bp
  40% of sequence in fragments shorter than   17809 bp
  45% of sequence in fragments shorter than   20313 bp
  50% of sequence in fragments shorter than   23129 bp
  55% of sequence in fragments shorter than   26153 bp
  60% of sequence in fragments shorter than   29608 bp
  65% of sequence in fragments shorter than   33708 bp
  70% of sequence in fragments shorter than   38271 bp
  75% of sequence in fragments shorter than   43893 bp
  80% of sequence in fragments shorter than   50715 bp
  85% of sequence in fragments shorter than   59952 bp
  90% of sequence in fragments shorter than   72922 bp
  95% of sequence in fragments shorter than   98591 bp

# FseI.NheI (GGCCGGCC+GCTAGC) double digest, 300 thousand fragments (13662+287384 sites), 9909 bp average fragment length
   5% of sequence in fragments shorter than    3156 bp
  10% of sequence in fragments shorter than    5243 bp
  15% of sequence in fragments shorter than    7001 bp
  20% of sequence in fragments shorter than    8598 bp
  25% of sequence in fragments shorter than   10158 bp
  30% of sequence in fragments shorter than   11686 bp
  35% of sequence in fragments shorter than   13242 bp
  40% of sequence in fragments shorter than   14842 bp
  45% of sequence in fragments shorter than   16547 bp
  50% of sequence in fragments shorter than   18351 bp
  55% of sequence in fragments shorter than   20276 bp
  60% of sequence in fragments shorter than   22386 bp
  65% of sequence in fragments shorter than   24720 bp
  70% of sequence in fragments shorter than   27370 bp
  75% of sequence in fragments shorter than   30468 bp
  80% of sequence in fragments shorter than   34247 bp
  85% of sequence in fragments shorter than   39195 bp
  90% of sequence in fragments shorter than   46353 bp
  95% of sequence in fragments shorter than   60603 bp

# AclI.NaeI (AACGTT+GCCGGC) double digest, 298 thousand fragments (172706+125692 sites), 9999 bp average fragment length
   5% of sequence in fragments shorter than    3457 bp
  10% of sequence in fragments shorter than    5583 bp
  15% of sequence in fragments shorter than    7442 bp
  20% of sequence in fragments shorter than    9199 bp
  25% of sequence in fragments shorter than   10915 bp
  30% of sequence in fragments shorter than   12609 bp
  35% of sequence in fragments shorter than   14386 bp
  40% of sequence in fragments shorter than   16218 bp
  45% of sequence in fragments shorter than   18092 bp
  50% of sequence in fragments shorter than   20160 bp
  55% of sequence in fragments shorter than   22349 bp
  60% of sequence in fragments shorter than   24773 bp
  65% of sequence in fragments shorter than   27443 bp
  70% of sequence in fragments shorter than   30502 bp
  75% of sequence in fragments shorter than   34131 bp
  80% of sequence in fragments shorter than   38558 bp
  85% of sequence in fragments shorter than   44253 bp
  90% of sequence in fragments shorter than   51888 bp
  95% of sequence in fragments shorter than   66449 bp

# KpnI.NotI (GGTACC+GCGGCCGC) double digest, 297 thousand fragments (287974+9795 sites), 10017 bp average fragment length
   5% of sequence in fragments shorter than    3316 bp
  10% of sequence in fragments shorter than    5262 bp
  15% of sequence in fragments shorter than    6960 bp
  20% of sequence in fragments shorter than    8546 bp
  25% of sequence in fragments shorter than   10080 bp
  30% of sequence in fragments shorter than   11615 bp
  35% of sequence in fragments shorter than   13182 bp
  40% of sequence in fragments shorter than   14775 bp
  45% of sequence in fragments shorter than   16417 bp
  50% of sequence in fragments shorter than   18214 bp
  55% of sequence in fragments shorter than   20111 bp
  60% of sequence in fragments shorter than   22170 bp
  65% of sequence in fragments shorter than   24507 bp
  70% of sequence in fragments shorter than   27156 bp
  75% of sequence in fragments shorter than   30241 bp
  80% of sequence in fragments shorter than   34042 bp
  85% of sequence in fragments shorter than   39063 bp
  90% of sequence in fragments shorter than   46464 bp
  95% of sequence in fragments shorter than   61484 bp

# KpnI.RsrII (GGTACC+CGGWCCG) double digest, 297 thousand fragments (287974+9764 sites), 10018 bp average fragment length
   5% of sequence in fragments shorter than    3309 bp
  10% of sequence in fragments shorter than    5249 bp
  15% of sequence in fragments shorter than    6945 bp
  20% of sequence in fragments shorter than    8515 bp
  25% of sequence in fragments shorter than   10032 bp
  30% of sequence in fragments shorter than   11560 bp
  35% of sequence in fragments shorter than   13129 bp
  40% of sequence in fragments shorter than   14726 bp
  45% of sequence in fragments shorter than   16343 bp
  50% of sequence in fragments shorter than   18127 bp
  55% of sequence in fragments shorter than   20012 bp
  60% of sequence in fragments shorter than   22065 bp
  65% of sequence in fragments shorter than   24389 bp
  70% of sequence in fragments shorter than   27024 bp
  75% of sequence in fragments shorter than   30126 bp
  80% of sequence in fragments shorter than   33925 bp
  85% of sequence in fragments shorter than   38921 bp
  90% of sequence in fragments shorter than   46232 bp
  95% of sequence in fragments shorter than   60848 bp

# AatII.SwaI (GACGTC+ATTTAAAT) double digest, 297 thousand fragments (71089+226602 sites), 10021 bp average fragment length
   5% of sequence in fragments shorter than    3515 bp
  10% of sequence in fragments shorter than    5348 bp
  15% of sequence in fragments shorter than    6952 bp
  20% of sequence in fragments shorter than    8468 bp
  25% of sequence in fragments shorter than    9953 bp
  30% of sequence in fragments shorter than   11441 bp
  35% of sequence in fragments shorter than   12953 bp
  40% of sequence in fragments shorter than   14528 bp
  45% of sequence in fragments shorter than   16178 bp
  50% of sequence in fragments shorter than   17959 bp
  55% of sequence in fragments shorter than   19871 bp
  60% of sequence in fragments shorter than   21976 bp
  65% of sequence in fragments shorter than   24309 bp
  70% of sequence in fragments shorter than   27019 bp
  75% of sequence in fragments shorter than   30210 bp
  80% of sequence in fragments shorter than   34153 bp
  85% of sequence in fragments shorter than   39422 bp
  90% of sequence in fragments shorter than   47350 bp
  95% of sequence in fragments shorter than   63544 bp

# KasI.SacII (GGCGCC+CCGCGG) double digest, 297 thousand fragments (230381+66901 sites), 10033 bp average fragment length
   5% of sequence in fragments shorter than    3684 bp
  10% of sequence in fragments shorter than    6190 bp
  15% of sequence in fragments shorter than    8569 bp
  20% of sequence in fragments shorter than   11009 bp
  25% of sequence in fragments shorter than   13530 bp
  30% of sequence in fragments shorter than   16271 bp
  35% of sequence in fragments shorter than   19171 bp
  40% of sequence in fragments shorter than   22350 bp
  45% of sequence in fragments shorter than   25772 bp
  50% of sequence in fragments shorter than   29563 bp
  55% of sequence in fragments shorter than   33870 bp
  60% of sequence in fragments shorter than   38626 bp
  65% of sequence in fragments shorter than   44177 bp
  70% of sequence in fragments shorter than   50638 bp
  75% of sequence in fragments shorter than   58249 bp
  80% of sequence in fragments shorter than   67858 bp
  85% of sequence in fragments shorter than   80769 bp
  90% of sequence in fragments shorter than  100302 bp
  95% of sequence in fragments shorter than  141927 bp

# NheI.NotI (GCTAGC+GCGGCCGC) double digest, 296 thousand fragments (287384+9795 sites), 10038 bp average fragment length
   5% of sequence in fragments shorter than    3206 bp
  10% of sequence in fragments shorter than    5325 bp
  15% of sequence in fragments shorter than    7108 bp
  20% of sequence in fragments shorter than    8736 bp
  25% of sequence in fragments shorter than   10331 bp
  30% of sequence in fragments shorter than   11878 bp
  35% of sequence in fragments shorter than   13470 bp
  40% of sequence in fragments shorter than   15109 bp
  45% of sequence in fragments shorter than   16841 bp
  50% of sequence in fragments shorter than   18677 bp
  55% of sequence in fragments shorter than   20624 bp
  60% of sequence in fragments shorter than   22768 bp
  65% of sequence in fragments shorter than   25155 bp
  70% of sequence in fragments shorter than   27849 bp
  75% of sequence in fragments shorter than   31037 bp
  80% of sequence in fragments shorter than   34875 bp
  85% of sequence in fragments shorter than   39928 bp
  90% of sequence in fragments shorter than   47268 bp
  95% of sequence in fragments shorter than   61950 bp

# NheI.RsrII (GCTAGC+CGGWCCG) double digest, 296 thousand fragments (287384+9764 sites), 10039 bp average fragment length
   5% of sequence in fragments shorter than    3195 bp
  10% of sequence in fragments shorter than    5306 bp
  15% of sequence in fragments shorter than    7087 bp
  20% of sequence in fragments shorter than    8712 bp
  25% of sequence in fragments shorter than   10308 bp
  30% of sequence in fragments shorter than   11844 bp
  35% of sequence in fragments shorter than   13435 bp
  40% of sequence in fragments shorter than   15065 bp
  45% of sequence in fragments shorter than   16784 bp
  50% of sequence in fragments shorter than   18614 bp
  55% of sequence in fragments shorter than   20544 bp
  60% of sequence in fragments shorter than   22686 bp
  65% of sequence in fragments shorter than   25079 bp
  70% of sequence in fragments shorter than   27771 bp
  75% of sequence in fragments shorter than   30951 bp
  80% of sequence in fragments shorter than   34751 bp
  85% of sequence in fragments shorter than   39775 bp
  90% of sequence in fragments shorter than   47085 bp
  95% of sequence in fragments shorter than   61593 bp

# BsrBI.PspXI (CCGCTC+VCTCGAGB) double digest, 294 thousand fragments (235523+59193 sites), 10125 bp average fragment length
   5% of sequence in fragments shorter than    3368 bp
  10% of sequence in fragments shorter than    5692 bp
  15% of sequence in fragments shorter than    7857 bp
  20% of sequence in fragments shorter than   10036 bp
  25% of sequence in fragments shorter than   12268 bp
  30% of sequence in fragments shorter than   14543 bp
  35% of sequence in fragments shorter than   16916 bp
  40% of sequence in fragments shorter than   19502 bp
  45% of sequence in fragments shorter than   22258 bp
  50% of sequence in fragments shorter than   25292 bp
  55% of sequence in fragments shorter than   28703 bp
  60% of sequence in fragments shorter than   32464 bp
  65% of sequence in fragments shorter than   36651 bp
  70% of sequence in fragments shorter than   41552 bp
  75% of sequence in fragments shorter than   47230 bp
  80% of sequence in fragments shorter than   54420 bp
  85% of sequence in fragments shorter than   64054 bp
  90% of sequence in fragments shorter than   77869 bp
  95% of sequence in fragments shorter than  104261 bp

# AclI.XhoI (AACGTT+CTCGAG) double digest, 293 thousand fragments (172706+121308 sites), 10149 bp average fragment length
   5% of sequence in fragments shorter than    3466 bp
  10% of sequence in fragments shorter than    5395 bp
  15% of sequence in fragments shorter than    7067 bp
  20% of sequence in fragments shorter than    8634 bp
  25% of sequence in fragments shorter than   10114 bp
  30% of sequence in fragments shorter than   11612 bp
  35% of sequence in fragments shorter than   13118 bp
  40% of sequence in fragments shorter than   14704 bp
  45% of sequence in fragments shorter than   16341 bp
  50% of sequence in fragments shorter than   18071 bp
  55% of sequence in fragments shorter than   19937 bp
  60% of sequence in fragments shorter than   21991 bp
  65% of sequence in fragments shorter than   24282 bp
  70% of sequence in fragments shorter than   26849 bp
  75% of sequence in fragments shorter than   29900 bp
  80% of sequence in fragments shorter than   33636 bp
  85% of sequence in fragments shorter than   38319 bp
  90% of sequence in fragments shorter than   45093 bp
  95% of sequence in fragments shorter than   57059 bp

# SacII.SwaI (CCGCGG+ATTTAAAT) double digest, 293 thousand fragments (66901+226602 sites), 10162 bp average fragment length
   5% of sequence in fragments shorter than    3755 bp
  10% of sequence in fragments shorter than    5815 bp
  15% of sequence in fragments shorter than    7644 bp
  20% of sequence in fragments shorter than    9347 bp
  25% of sequence in fragments shorter than   10982 bp
  30% of sequence in fragments shorter than   12613 bp
  35% of sequence in fragments shorter than   14301 bp
  40% of sequence in fragments shorter than   16089 bp
  45% of sequence in fragments shorter than   17966 bp
  50% of sequence in fragments shorter than   19959 bp
  55% of sequence in fragments shorter than   22124 bp
  60% of sequence in fragments shorter than   24496 bp
  65% of sequence in fragments shorter than   27234 bp
  70% of sequence in fragments shorter than   30321 bp
  75% of sequence in fragments shorter than   34013 bp
  80% of sequence in fragments shorter than   38635 bp
  85% of sequence in fragments shorter than   44713 bp
  90% of sequence in fragments shorter than   53993 bp
  95% of sequence in fragments shorter than   73984 bp

# AscI.KpnI (GGCGCGCC+GGTACC) double digest, 292 thousand fragments (4751+287974 sites), 10190 bp average fragment length
   5% of sequence in fragments shorter than    3365 bp
  10% of sequence in fragments shorter than    5343 bp
  15% of sequence in fragments shorter than    7065 bp
  20% of sequence in fragments shorter than    8664 bp
  25% of sequence in fragments shorter than   10220 bp
  30% of sequence in fragments shorter than   11770 bp
  35% of sequence in fragments shorter than   13352 bp
  40% of sequence in fragments shorter than   14960 bp
  45% of sequence in fragments shorter than   16618 bp
  50% of sequence in fragments shorter than   18421 bp
  55% of sequence in fragments shorter than   20330 bp
  60% of sequence in fragments shorter than   22403 bp
  65% of sequence in fragments shorter than   24778 bp
  70% of sequence in fragments shorter than   27453 bp
  75% of sequence in fragments shorter than   30614 bp
  80% of sequence in fragments shorter than   34445 bp
  85% of sequence in fragments shorter than   39568 bp
  90% of sequence in fragments shorter than   47032 bp
  95% of sequence in fragments shorter than   62051 bp

# AscI.NheI (GGCGCGCC+GCTAGC) double digest, 291 thousand fragments (4751+287384 sites), 10212 bp average fragment length
   5% of sequence in fragments shorter than    3254 bp
  10% of sequence in fragments shorter than    5397 bp
  15% of sequence in fragments shorter than    7208 bp
  20% of sequence in fragments shorter than    8867 bp
  25% of sequence in fragments shorter than   10477 bp
  30% of sequence in fragments shorter than   12046 bp
  35% of sequence in fragments shorter than   13661 bp
  40% of sequence in fragments shorter than   15322 bp
  45% of sequence in fragments shorter than   17089 bp
  50% of sequence in fragments shorter than   18940 bp
  55% of sequence in fragments shorter than   20928 bp
  60% of sequence in fragments shorter than   23143 bp
  65% of sequence in fragments shorter than   25540 bp
  70% of sequence in fragments shorter than   28304 bp
  75% of sequence in fragments shorter than   31534 bp
  80% of sequence in fragments shorter than   35503 bp
  85% of sequence in fragments shorter than   40698 bp
  90% of sequence in fragments shorter than   48080 bp
  95% of sequence in fragments shorter than   63490 bp

# PacI.SnaBI (TTAATTAA+TACGTA) double digest, 291 thousand fragments (159455+132299 sites), 10225 bp average fragment length
   5% of sequence in fragments shorter than    3644 bp
  10% of sequence in fragments shorter than    5591 bp
  15% of sequence in fragments shorter than    7307 bp
  20% of sequence in fragments shorter than    8936 bp
  25% of sequence in fragments shorter than   10527 bp
  30% of sequence in fragments shorter than   12102 bp
  35% of sequence in fragments shorter than   13775 bp
  40% of sequence in fragments shorter than   15510 bp
  45% of sequence in fragments shorter than   17326 bp
  50% of sequence in fragments shorter than   19302 bp
  55% of sequence in fragments shorter than   21454 bp
  60% of sequence in fragments shorter than   23800 bp
  65% of sequence in fragments shorter than   26427 bp
  70% of sequence in fragments shorter than   29567 bp
  75% of sequence in fragments shorter than   33319 bp
  80% of sequence in fragments shorter than   38006 bp
  85% of sequence in fragments shorter than   44531 bp
  90% of sequence in fragments shorter than   54712 bp
  95% of sequence in fragments shorter than   77179 bp

# Hpy99I.SnaBI (CGWCG+TACGTA) double digest, 289 thousand fragments (157917+132299 sites), 10282 bp average fragment length
   5% of sequence in fragments shorter than    3773 bp
  10% of sequence in fragments shorter than    6018 bp
  15% of sequence in fragments shorter than    7951 bp
  20% of sequence in fragments shorter than    9784 bp
  25% of sequence in fragments shorter than   11592 bp
  30% of sequence in fragments shorter than   13400 bp
  35% of sequence in fragments shorter than   15244 bp
  40% of sequence in fragments shorter than   17197 bp
  45% of sequence in fragments shorter than   19223 bp
  50% of sequence in fragments shorter than   21439 bp
  55% of sequence in fragments shorter than   23786 bp
  60% of sequence in fragments shorter than   26351 bp
  65% of sequence in fragments shorter than   29221 bp
  70% of sequence in fragments shorter than   32528 bp
  75% of sequence in fragments shorter than   36307 bp
  80% of sequence in fragments shorter than   40944 bp
  85% of sequence in fragments shorter than   46936 bp
  90% of sequence in fragments shorter than   55486 bp
  95% of sequence in fragments shorter than   72680 bp

# KasI.PspXI (GGCGCC+VCTCGAGB) double digest, 289 thousand fragments (230381+59193 sites), 10301 bp average fragment length
   5% of sequence in fragments shorter than    3426 bp
  10% of sequence in fragments shorter than    5584 bp
  15% of sequence in fragments shorter than    7588 bp
  20% of sequence in fragments shorter than    9620 bp
  25% of sequence in fragments shorter than   11672 bp
  30% of sequence in fragments shorter than   13890 bp
  35% of sequence in fragments shorter than   16303 bp
  40% of sequence in fragments shorter than   18794 bp
  45% of sequence in fragments shorter than   21570 bp
  50% of sequence in fragments shorter than   24642 bp
  55% of sequence in fragments shorter than   27962 bp
  60% of sequence in fragments shorter than   31757 bp
  65% of sequence in fragments shorter than   36212 bp
  70% of sequence in fragments shorter than   41364 bp
  75% of sequence in fragments shorter than   47693 bp
  80% of sequence in fragments shorter than   55265 bp
  85% of sequence in fragments shorter than   65315 bp
  90% of sequence in fragments shorter than   80695 bp
  95% of sequence in fragments shorter than  110972 bp

# AgeI.BsrBI (ACCGGT+CCGCTC) double digest, 288 thousand fragments (53386+235523 sites), 10329 bp average fragment length
   5% of sequence in fragments shorter than    3482 bp
  10% of sequence in fragments shorter than    5833 bp
  15% of sequence in fragments shorter than    8006 bp
  20% of sequence in fragments shorter than   10177 bp
  25% of sequence in fragments shorter than   12364 bp
  30% of sequence in fragments shorter than   14618 bp
  35% of sequence in fragments shorter than   16967 bp
  40% of sequence in fragments shorter than   19467 bp
  45% of sequence in fragments shorter than   22087 bp
  50% of sequence in fragments shorter than   25027 bp
  55% of sequence in fragments shorter than   28329 bp
  60% of sequence in fragments shorter than   31919 bp
  65% of sequence in fragments shorter than   35954 bp
  70% of sequence in fragments shorter than   40523 bp
  75% of sequence in fragments shorter than   46069 bp
  80% of sequence in fragments shorter than   52685 bp
  85% of sequence in fragments shorter than   61647 bp
  90% of sequence in fragments shorter than   75087 bp
  95% of sequence in fragments shorter than   99423 bp

# PspXI.SwaI (VCTCGAGB+ATTTAAAT) double digest, 285 thousand fragments (59193+226602 sites), 10437 bp average fragment length
   5% of sequence in fragments shorter than    3653 bp
  10% of sequence in fragments shorter than    5562 bp
  15% of sequence in fragments shorter than    7240 bp
  20% of sequence in fragments shorter than    8803 bp
  25% of sequence in fragments shorter than   10326 bp
  30% of sequence in fragments shorter than   11874 bp
  35% of sequence in fragments shorter than   13435 bp
  40% of sequence in fragments shorter than   15049 bp
  45% of sequence in fragments shorter than   16758 bp
  50% of sequence in fragments shorter than   18584 bp
  55% of sequence in fragments shorter than   20575 bp
  60% of sequence in fragments shorter than   22751 bp
  65% of sequence in fragments shorter than   25206 bp
  70% of sequence in fragments shorter than   28014 bp
  75% of sequence in fragments shorter than   31403 bp
  80% of sequence in fragments shorter than   35567 bp
  85% of sequence in fragments shorter than   41119 bp
  90% of sequence in fragments shorter than   49478 bp
  95% of sequence in fragments shorter than   67258 bp

# NaeI.PacI (GCCGGC+TTAATTAA) double digest, 284 thousand fragments (125692+159455 sites), 10459 bp average fragment length
   5% of sequence in fragments shorter than    3934 bp
  10% of sequence in fragments shorter than    6127 bp
  15% of sequence in fragments shorter than    8027 bp
  20% of sequence in fragments shorter than    9829 bp
  25% of sequence in fragments shorter than   11609 bp
  30% of sequence in fragments shorter than   13388 bp
  35% of sequence in fragments shorter than   15222 bp
  40% of sequence in fragments shorter than   17075 bp
  45% of sequence in fragments shorter than   19093 bp
  50% of sequence in fragments shorter than   21216 bp
  55% of sequence in fragments shorter than   23496 bp
  60% of sequence in fragments shorter than   26055 bp
  65% of sequence in fragments shorter than   28828 bp
  70% of sequence in fragments shorter than   32014 bp
  75% of sequence in fragments shorter than   35671 bp
  80% of sequence in fragments shorter than   40151 bp
  85% of sequence in fragments shorter than   46239 bp
  90% of sequence in fragments shorter than   54979 bp
  95% of sequence in fragments shorter than   74465 bp

# AgeI.KasI (ACCGGT+GGCGCC) double digest, 283 thousand fragments (53386+230381 sites), 10512 bp average fragment length
   5% of sequence in fragments shorter than    3518 bp
  10% of sequence in fragments shorter than    5733 bp
  15% of sequence in fragments shorter than    7782 bp
  20% of sequence in fragments shorter than    9805 bp
  25% of sequence in fragments shorter than   11859 bp
  30% of sequence in fragments shorter than   14037 bp
  35% of sequence in fragments shorter than   16360 bp
  40% of sequence in fragments shorter than   18803 bp
  45% of sequence in fragments shorter than   21462 bp
  50% of sequence in fragments shorter than   24370 bp
  55% of sequence in fragments shorter than   27533 bp
  60% of sequence in fragments shorter than   31150 bp
  65% of sequence in fragments shorter than   35296 bp
  70% of sequence in fragments shorter than   40064 bp
  75% of sequence in fragments shorter than   45662 bp
  80% of sequence in fragments shorter than   52658 bp
  85% of sequence in fragments shorter than   62036 bp
  90% of sequence in fragments shorter than   75801 bp
  95% of sequence in fragments shorter than  104686 bp

# Hpy99I.NaeI (CGWCG+GCCGGC) double digest, 283 thousand fragments (157917+125692 sites), 10520 bp average fragment length
   5% of sequence in fragments shorter than    3900 bp
  10% of sequence in fragments shorter than    6839 bp
  15% of sequence in fragments shorter than    9641 bp
  20% of sequence in fragments shorter than   12476 bp
  25% of sequence in fragments shorter than   15473 bp
  30% of sequence in fragments shorter than   18553 bp
  35% of sequence in fragments shorter than   21866 bp
  40% of sequence in fragments shorter than   25420 bp
  45% of sequence in fragments shorter than   29231 bp
  50% of sequence in fragments shorter than   33383 bp
  55% of sequence in fragments shorter than   38153 bp
  60% of sequence in fragments shorter than   43442 bp
  65% of sequence in fragments shorter than   49451 bp
  70% of sequence in fragments shorter than   56276 bp
  75% of sequence in fragments shorter than   64512 bp
  80% of sequence in fragments shorter than   74621 bp
  85% of sequence in fragments shorter than   88917 bp
  90% of sequence in fragments shorter than  110169 bp
  95% of sequence in fragments shorter than  152083 bp

# PacI.XhoI (TTAATTAA+CTCGAG) double digest, 280 thousand fragments (159455+121308 sites), 10625 bp average fragment length
   5% of sequence in fragments shorter than    3913 bp
  10% of sequence in fragments shorter than    5892 bp
  15% of sequence in fragments shorter than    7588 bp
  20% of sequence in fragments shorter than    9177 bp
  25% of sequence in fragments shorter than   10734 bp
  30% of sequence in fragments shorter than   12268 bp
  35% of sequence in fragments shorter than   13827 bp
  40% of sequence in fragments shorter than   15452 bp
  45% of sequence in fragments shorter than   17107 bp
  50% of sequence in fragments shorter than   18919 bp
  55% of sequence in fragments shorter than   20876 bp
  60% of sequence in fragments shorter than   22988 bp
  65% of sequence in fragments shorter than   25331 bp
  70% of sequence in fragments shorter than   27971 bp
  75% of sequence in fragments shorter than   31119 bp
  80% of sequence in fragments shorter than   34982 bp
  85% of sequence in fragments shorter than   39887 bp
  90% of sequence in fragments shorter than   47224 bp
  95% of sequence in fragments shorter than   61778 bp

# AgeI.SwaI (ACCGGT+ATTTAAAT) double digest, 279 thousand fragments (53386+226602 sites), 10654 bp average fragment length
   5% of sequence in fragments shorter than    3715 bp
  10% of sequence in fragments shorter than    5629 bp
  15% of sequence in fragments shorter than    7344 bp
  20% of sequence in fragments shorter than    8941 bp
  25% of sequence in fragments shorter than   10503 bp
  30% of sequence in fragments shorter than   12071 bp
  35% of sequence in fragments shorter than   13678 bp
  40% of sequence in fragments shorter than   15370 bp
  45% of sequence in fragments shorter than   17184 bp
  50% of sequence in fragments shorter than   19113 bp
  55% of sequence in fragments shorter than   21197 bp
  60% of sequence in fragments shorter than   23466 bp
  65% of sequence in fragments shorter than   26070 bp
  70% of sequence in fragments shorter than   29119 bp
  75% of sequence in fragments shorter than   32695 bp
  80% of sequence in fragments shorter than   37274 bp
  85% of sequence in fragments shorter than   43246 bp
  90% of sequence in fragments shorter than   52415 bp
  95% of sequence in fragments shorter than   74040 bp

# Hpy99I.XhoI (CGWCG+CTCGAG) double digest, 278 thousand fragments (157917+121308 sites), 10686 bp average fragment length
   5% of sequence in fragments shorter than    3750 bp
  10% of sequence in fragments shorter than    6179 bp
  15% of sequence in fragments shorter than    8379 bp
  20% of sequence in fragments shorter than   10546 bp
  25% of sequence in fragments shorter than   12737 bp
  30% of sequence in fragments shorter than   15005 bp
  35% of sequence in fragments shorter than   17341 bp
  40% of sequence in fragments shorter than   19878 bp
  45% of sequence in fragments shorter than   22572 bp
  50% of sequence in fragments shorter than   25537 bp
  55% of sequence in fragments shorter than   28753 bp
  60% of sequence in fragments shorter than   32346 bp
  65% of sequence in fragments shorter than   36421 bp
  70% of sequence in fragments shorter than   41127 bp
  75% of sequence in fragments shorter than   46830 bp
  80% of sequence in fragments shorter than   53922 bp
  85% of sequence in fragments shorter than   63115 bp
  90% of sequence in fragments shorter than   76301 bp
  95% of sequence in fragments shorter than  101985 bp

# BsrBI.PmeI (CCGCTC+GTTTAAAC) double digest, 277 thousand fragments (235523+42094 sites), 10750 bp average fragment length
   5% of sequence in fragments shorter than    3702 bp
  10% of sequence in fragments shorter than    6104 bp
  15% of sequence in fragments shorter than    8269 bp
  20% of sequence in fragments shorter than   10381 bp
  25% of sequence in fragments shorter than   12504 bp
  30% of sequence in fragments shorter than   14655 bp
  35% of sequence in fragments shorter than   16918 bp
  40% of sequence in fragments shorter than   19331 bp
  45% of sequence in fragments shorter than   21844 bp
  50% of sequence in fragments shorter than   24545 bp
  55% of sequence in fragments shorter than   27589 bp
  60% of sequence in fragments shorter than   30866 bp
  65% of sequence in fragments shorter than   34591 bp
  70% of sequence in fragments shorter than   38833 bp
  75% of sequence in fragments shorter than   43853 bp
  80% of sequence in fragments shorter than   49778 bp
  85% of sequence in fragments shorter than   57896 bp
  90% of sequence in fragments shorter than   69765 bp
  95% of sequence in fragments shorter than   91608 bp

# KasI.PmeI (GGCGCC+GTTTAAAC) double digest, 272 thousand fragments (230381+42094 sites), 10949 bp average fragment length
   5% of sequence in fragments shorter than    3724 bp
  10% of sequence in fragments shorter than    6003 bp
  15% of sequence in fragments shorter than    8056 bp
  20% of sequence in fragments shorter than   10081 bp
  25% of sequence in fragments shorter than   12119 bp
  30% of sequence in fragments shorter than   14266 bp
  35% of sequence in fragments shorter than   16529 bp
  40% of sequence in fragments shorter than   18899 bp
  45% of sequence in fragments shorter than   21511 bp
  50% of sequence in fragments shorter than   24357 bp
  55% of sequence in fragments shorter than   27381 bp
  60% of sequence in fragments shorter than   30889 bp
  65% of sequence in fragments shorter than   34868 bp
  70% of sequence in fragments shorter than   39295 bp
  75% of sequence in fragments shorter than   44821 bp
  80% of sequence in fragments shorter than   51366 bp
  85% of sequence in fragments shorter than   60115 bp
  90% of sequence in fragments shorter than   73465 bp
  95% of sequence in fragments shorter than   98824 bp

# PmeI.SwaI (GTTTAAAC+ATTTAAAT) double digest, 268 thousand fragments (42094+226602 sites), 11102 bp average fragment length
   5% of sequence in fragments shorter than    3767 bp
  10% of sequence in fragments shorter than    5792 bp
  15% of sequence in fragments shorter than    7616 bp
  20% of sequence in fragments shorter than    9327 bp
  25% of sequence in fragments shorter than   11010 bp
  30% of sequence in fragments shorter than   12741 bp
  35% of sequence in fragments shorter than   14522 bp
  40% of sequence in fragments shorter than   16447 bp
  45% of sequence in fragments shorter than   18497 bp
  50% of sequence in fragments shorter than   20695 bp
  55% of sequence in fragments shorter than   23117 bp
  60% of sequence in fragments shorter than   25886 bp
  65% of sequence in fragments shorter than   29134 bp
  70% of sequence in fragments shorter than   33004 bp
  75% of sequence in fragments shorter than   37767 bp
  80% of sequence in fragments shorter than   44005 bp
  85% of sequence in fragments shorter than   53162 bp
  90% of sequence in fragments shorter than   68128 bp
  95% of sequence in fragments shorter than  105973 bp

# BsrBI.SalI (CCGCTC+GTCGAC) double digest, 266 thousand fragments (235523+31396 sites), 11181 bp average fragment length
   5% of sequence in fragments shorter than    3788 bp
  10% of sequence in fragments shorter than    6346 bp
  15% of sequence in fragments shorter than    8711 bp
  20% of sequence in fragments shorter than   11089 bp
  25% of sequence in fragments shorter than   13476 bp
  30% of sequence in fragments shorter than   15952 bp
  35% of sequence in fragments shorter than   18559 bp
  40% of sequence in fragments shorter than   21339 bp
  45% of sequence in fragments shorter than   24293 bp
  50% of sequence in fragments shorter than   27640 bp
  55% of sequence in fragments shorter than   31210 bp
  60% of sequence in fragments shorter than   35237 bp
  65% of sequence in fragments shorter than   39747 bp
  70% of sequence in fragments shorter than   44951 bp
  75% of sequence in fragments shorter than   50921 bp
  80% of sequence in fragments shorter than   58558 bp
  85% of sequence in fragments shorter than   68501 bp
  90% of sequence in fragments shorter than   83031 bp
  95% of sequence in fragments shorter than  110498 bp

# KasI.SalI (GGCGCC+GTCGAC) double digest, 261 thousand fragments (230381+31396 sites), 11396 bp average fragment length
   5% of sequence in fragments shorter than    3832 bp
  10% of sequence in fragments shorter than    6238 bp
  15% of sequence in fragments shorter than    8455 bp
  20% of sequence in fragments shorter than   10664 bp
  25% of sequence in fragments shorter than   12946 bp
  30% of sequence in fragments shorter than   15411 bp
  35% of sequence in fragments shorter than   17972 bp
  40% of sequence in fragments shorter than   20784 bp
  45% of sequence in fragments shorter than   23783 bp
  50% of sequence in fragments shorter than   27026 bp
  55% of sequence in fragments shorter than   30716 bp
  60% of sequence in fragments shorter than   34787 bp
  65% of sequence in fragments shorter than   39478 bp
  70% of sequence in fragments shorter than   44818 bp
  75% of sequence in fragments shorter than   51255 bp
  80% of sequence in fragments shorter than   59394 bp
  85% of sequence in fragments shorter than   69913 bp
  90% of sequence in fragments shorter than   86899 bp
  95% of sequence in fragments shorter than  118572 bp

# AclI.ClaI (AACGTT+ATCGAT) double digest, 258 thousand fragments (172706+86477 sites), 11515 bp average fragment length
   5% of sequence in fragments shorter than    3943 bp
  10% of sequence in fragments shorter than    6126 bp
  15% of sequence in fragments shorter than    7980 bp
  20% of sequence in fragments shorter than    9712 bp
  25% of sequence in fragments shorter than   11362 bp
  30% of sequence in fragments shorter than   13025 bp
  35% of sequence in fragments shorter than   14729 bp
  40% of sequence in fragments shorter than   16503 bp
  45% of sequence in fragments shorter than   18324 bp
  50% of sequence in fragments shorter than   20294 bp
  55% of sequence in fragments shorter than   22394 bp
  60% of sequence in fragments shorter than   24665 bp
  65% of sequence in fragments shorter than   27181 bp
  70% of sequence in fragments shorter than   30071 bp
  75% of sequence in fragments shorter than   33452 bp
  80% of sequence in fragments shorter than   37612 bp
  85% of sequence in fragments shorter than   43070 bp
  90% of sequence in fragments shorter than   50570 bp
  95% of sequence in fragments shorter than   64519 bp

# SalI.SwaI (GTCGAC+ATTTAAAT) double digest, 257 thousand fragments (31396+226602 sites), 11563 bp average fragment length
   5% of sequence in fragments shorter than    3985 bp
  10% of sequence in fragments shorter than    6107 bp
  15% of sequence in fragments shorter than    7991 bp
  20% of sequence in fragments shorter than    9766 bp
  25% of sequence in fragments shorter than   11493 bp
  30% of sequence in fragments shorter than   13220 bp
  35% of sequence in fragments shorter than   15014 bp
  40% of sequence in fragments shorter than   16957 bp
  45% of sequence in fragments shorter than   18981 bp
  50% of sequence in fragments shorter than   21185 bp
  55% of sequence in fragments shorter than   23539 bp
  60% of sequence in fragments shorter than   26172 bp
  65% of sequence in fragments shorter than   29253 bp
  70% of sequence in fragments shorter than   32864 bp
  75% of sequence in fragments shorter than   37282 bp
  80% of sequence in fragments shorter than   42845 bp
  85% of sequence in fragments shorter than   50430 bp
  90% of sequence in fragments shorter than   62224 bp
  95% of sequence in fragments shorter than   90524 bp

# NaeI.SnaBI (GCCGGC+TACGTA) double digest, 257 thousand fragments (125692+132299 sites), 11564 bp average fragment length
   5% of sequence in fragments shorter than    4172 bp
  10% of sequence in fragments shorter than    6650 bp
  15% of sequence in fragments shorter than    8827 bp
  20% of sequence in fragments shorter than   10899 bp
  25% of sequence in fragments shorter than   12917 bp
  30% of sequence in fragments shorter than   14969 bp
  35% of sequence in fragments shorter than   17061 bp
  40% of sequence in fragments shorter than   19260 bp
  45% of sequence in fragments shorter than   21585 bp
  50% of sequence in fragments shorter than   24050 bp
  55% of sequence in fragments shorter than   26681 bp
  60% of sequence in fragments shorter than   29618 bp
  65% of sequence in fragments shorter than   32806 bp
  70% of sequence in fragments shorter than   36462 bp
  75% of sequence in fragments shorter than   40831 bp
  80% of sequence in fragments shorter than   46132 bp
  85% of sequence in fragments shorter than   53156 bp
  90% of sequence in fragments shorter than   63830 bp
  95% of sequence in fragments shorter than   84690 bp

# BsrBI.MluI (CCGCTC+ACGCGT) double digest, 256 thousand fragments (235523+21415 sites), 11616 bp average fragment length
   5% of sequence in fragments shorter than    3912 bp
  10% of sequence in fragments shorter than    6651 bp
  15% of sequence in fragments shorter than    9220 bp
  20% of sequence in fragments shorter than   11797 bp
  25% of sequence in fragments shorter than   14454 bp
  30% of sequence in fragments shorter than   17183 bp
  35% of sequence in fragments shorter than   20119 bp
  40% of sequence in fragments shorter than   23170 bp
  45% of sequence in fragments shorter than   26579 bp
  50% of sequence in fragments shorter than   30305 bp
  55% of sequence in fragments shorter than   34344 bp
  60% of sequence in fragments shorter than   38802 bp
  65% of sequence in fragments shorter than   44001 bp
  70% of sequence in fragments shorter than   49787 bp
  75% of sequence in fragments shorter than   56718 bp
  80% of sequence in fragments shorter than   65517 bp
  85% of sequence in fragments shorter than   77068 bp
  90% of sequence in fragments shorter than   93990 bp
  95% of sequence in fragments shorter than  124930 bp

# SnaBI.XhoI (TACGTA+CTCGAG) double digest, 253 thousand fragments (132299+121308 sites), 11767 bp average fragment length
   5% of sequence in fragments shorter than    4173 bp
  10% of sequence in fragments shorter than    6362 bp
  15% of sequence in fragments shorter than    8252 bp
  20% of sequence in fragments shorter than   10022 bp
  25% of sequence in fragments shorter than   11735 bp
  30% of sequence in fragments shorter than   13434 bp
  35% of sequence in fragments shorter than   15184 bp
  40% of sequence in fragments shorter than   16999 bp
  45% of sequence in fragments shorter than   18904 bp
  50% of sequence in fragments shorter than   20910 bp
  55% of sequence in fragments shorter than   23090 bp
  60% of sequence in fragments shorter than   25440 bp
  65% of sequence in fragments shorter than   28073 bp
  70% of sequence in fragments shorter than   31090 bp
  75% of sequence in fragments shorter than   34539 bp
  80% of sequence in fragments shorter than   38773 bp
  85% of sequence in fragments shorter than   44417 bp
  90% of sequence in fragments shorter than   52123 bp
  95% of sequence in fragments shorter than   67948 bp

# KasI.MluI (GGCGCC+ACGCGT) double digest, 251 thousand fragments (230381+21415 sites), 11848 bp average fragment length
   5% of sequence in fragments shorter than    3940 bp
  10% of sequence in fragments shorter than    6481 bp
  15% of sequence in fragments shorter than    8888 bp
  20% of sequence in fragments shorter than   11294 bp
  25% of sequence in fragments shorter than   13861 bp
  30% of sequence in fragments shorter than   16627 bp
  35% of sequence in fragments shorter than   19471 bp
  40% of sequence in fragments shorter than   22630 bp
  45% of sequence in fragments shorter than   25961 bp
  50% of sequence in fragments shorter than   29694 bp
  55% of sequence in fragments shorter than   33916 bp
  60% of sequence in fragments shorter than   38548 bp
  65% of sequence in fragments shorter than   43999 bp
  70% of sequence in fragments shorter than   50201 bp
  75% of sequence in fragments shorter than   57656 bp
  80% of sequence in fragments shorter than   67286 bp
  85% of sequence in fragments shorter than   79827 bp
  90% of sequence in fragments shorter than   99164 bp
  95% of sequence in fragments shorter than  137663 bp

# BsrBI.SgrAI (CCGCTC+CRCCGGYG) double digest, 250 thousand fragments (235523+15270 sites), 11901 bp average fragment length
   5% of sequence in fragments shorter than    4062 bp
  10% of sequence in fragments shorter than    6931 bp
  15% of sequence in fragments shorter than    9625 bp
  20% of sequence in fragments shorter than   12342 bp
  25% of sequence in fragments shorter than   15096 bp
  30% of sequence in fragments shorter than   17980 bp
  35% of sequence in fragments shorter than   21047 bp
  40% of sequence in fragments shorter than   24271 bp
  45% of sequence in fragments shorter than   27889 bp
  50% of sequence in fragments shorter than   31779 bp
  55% of sequence in fragments shorter than   36048 bp
  60% of sequence in fragments shorter than   40761 bp
  65% of sequence in fragments shorter than   46023 bp
  70% of sequence in fragments shorter than   52162 bp
  75% of sequence in fragments shorter than   59770 bp
  80% of sequence in fragments shorter than   68730 bp
  85% of sequence in fragments shorter than   80627 bp
  90% of sequence in fragments shorter than   98324 bp
  95% of sequence in fragments shorter than  132858 bp

# BsrBI.NruI (CCGCTC+TCGCGA) double digest, 249 thousand fragments (235523+14872 sites), 11920 bp average fragment length
   5% of sequence in fragments shorter than    4036 bp
  10% of sequence in fragments shorter than    6845 bp
  15% of sequence in fragments shorter than    9461 bp
  20% of sequence in fragments shorter than   12100 bp
  25% of sequence in fragments shorter than   14803 bp
  30% of sequence in fragments shorter than   17608 bp
  35% of sequence in fragments shorter than   20603 bp
  40% of sequence in fragments shorter than   23734 bp
  45% of sequence in fragments shorter than   27232 bp
  50% of sequence in fragments shorter than   30999 bp
  55% of sequence in fragments shorter than   35137 bp
  60% of sequence in fragments shorter than   39879 bp
  65% of sequence in fragments shorter than   45011 bp
  70% of sequence in fragments shorter than   50887 bp
  75% of sequence in fragments shorter than   58101 bp
  80% of sequence in fragments shorter than   67055 bp
  85% of sequence in fragments shorter than   78622 bp
  90% of sequence in fragments shorter than   95784 bp
  95% of sequence in fragments shorter than  129387 bp

# BsrBI.PvuI (CCGCTC+CGATCG) double digest, 249 thousand fragments (235523+14186 sites), 11953 bp average fragment length
   5% of sequence in fragments shorter than    3999 bp
  10% of sequence in fragments shorter than    6826 bp
  15% of sequence in fragments shorter than    9452 bp
  20% of sequence in fragments shorter than   12114 bp
  25% of sequence in fragments shorter than   14813 bp
  30% of sequence in fragments shorter than   17600 bp
  35% of sequence in fragments shorter than   20583 bp
  40% of sequence in fragments shorter than   23723 bp
  45% of sequence in fragments shorter than   27256 bp
  50% of sequence in fragments shorter than   31002 bp
  55% of sequence in fragments shorter than   35178 bp
  60% of sequence in fragments shorter than   39850 bp
  65% of sequence in fragments shorter than   45012 bp
  70% of sequence in fragments shorter than   50962 bp
  75% of sequence in fragments shorter than   58251 bp
  80% of sequence in fragments shorter than   67360 bp
  85% of sequence in fragments shorter than   78942 bp
  90% of sequence in fragments shorter than   96121 bp
  95% of sequence in fragments shorter than  129070 bp

# BsrBI.FseI (CCGCTC+GGCCGGCC) double digest, 248 thousand fragments (235523+13662 sites), 11978 bp average fragment length
   5% of sequence in fragments shorter than    4060 bp
  10% of sequence in fragments shorter than    6943 bp
  15% of sequence in fragments shorter than    9652 bp
  20% of sequence in fragments shorter than   12380 bp
  25% of sequence in fragments shorter than   15162 bp
  30% of sequence in fragments shorter than   18085 bp
  35% of sequence in fragments shorter than   21194 bp
  40% of sequence in fragments shorter than   24481 bp
  45% of sequence in fragments shorter than   28158 bp
  50% of sequence in fragments shorter than   32068 bp
  55% of sequence in fragments shorter than   36442 bp
  60% of sequence in fragments shorter than   41153 bp
  65% of sequence in fragments shorter than   46525 bp
  70% of sequence in fragments shorter than   52793 bp
  75% of sequence in fragments shorter than   60404 bp
  80% of sequence in fragments shorter than   69456 bp
  85% of sequence in fragments shorter than   81805 bp
  90% of sequence in fragments shorter than   99710 bp
  95% of sequence in fragments shorter than  134312 bp

# MluI.SwaI (ACGCGT+ATTTAAAT) double digest, 247 thousand fragments (21415+226602 sites), 12029 bp average fragment length
   5% of sequence in fragments shorter than    4148 bp
  10% of sequence in fragments shorter than    6379 bp
  15% of sequence in fragments shorter than    8357 bp
  20% of sequence in fragments shorter than   10191 bp
  25% of sequence in fragments shorter than   12008 bp
  30% of sequence in fragments shorter than   13844 bp
  35% of sequence in fragments shorter than   15747 bp
  40% of sequence in fragments shorter than   17755 bp
  45% of sequence in fragments shorter than   19887 bp
  50% of sequence in fragments shorter than   22168 bp
  55% of sequence in fragments shorter than   24646 bp
  60% of sequence in fragments shorter than   27482 bp
  65% of sequence in fragments shorter than   30710 bp
  70% of sequence in fragments shorter than   34390 bp
  75% of sequence in fragments shorter than   38882 bp
  80% of sequence in fragments shorter than   44394 bp
  85% of sequence in fragments shorter than   52140 bp
  90% of sequence in fragments shorter than   64041 bp
  95% of sequence in fragments shorter than   91038 bp

# NaeI.XhoI (GCCGGC+CTCGAG) double digest, 246 thousand fragments (125692+121308 sites), 12079 bp average fragment length
   5% of sequence in fragments shorter than    4175 bp
  10% of sequence in fragments shorter than    6893 bp
  15% of sequence in fragments shorter than    9360 bp
  20% of sequence in fragments shorter than   11795 bp
  25% of sequence in fragments shorter than   14302 bp
  30% of sequence in fragments shorter than   16953 bp
  35% of sequence in fragments shorter than   19713 bp
  40% of sequence in fragments shorter than   22668 bp
  45% of sequence in fragments shorter than   25848 bp
  50% of sequence in fragments shorter than   29363 bp
  55% of sequence in fragments shorter than   33200 bp
  60% of sequence in fragments shorter than   37450 bp
  65% of sequence in fragments shorter than   42472 bp
  70% of sequence in fragments shorter than   48236 bp
  75% of sequence in fragments shorter than   55025 bp
  80% of sequence in fragments shorter than   63417 bp
  85% of sequence in fragments shorter than   74927 bp
  90% of sequence in fragments shorter than   92373 bp
  95% of sequence in fragments shorter than  128056 bp

# ClaI.PacI (ATCGAT+TTAATTAA) double digest, 245 thousand fragments (86477+159455 sites), 12131 bp average fragment length
   5% of sequence in fragments shorter than    4377 bp
  10% of sequence in fragments shorter than    6635 bp
  15% of sequence in fragments shorter than    8586 bp
  20% of sequence in fragments shorter than   10411 bp
  25% of sequence in fragments shorter than   12248 bp
  30% of sequence in fragments shorter than   14108 bp
  35% of sequence in fragments shorter than   16001 bp
  40% of sequence in fragments shorter than   17932 bp
  45% of sequence in fragments shorter than   20042 bp
  50% of sequence in fragments shorter than   22293 bp
  55% of sequence in fragments shorter than   24648 bp
  60% of sequence in fragments shorter than   27305 bp
  65% of sequence in fragments shorter than   30233 bp
  70% of sequence in fragments shorter than   33638 bp
  75% of sequence in fragments shorter than   37709 bp
  80% of sequence in fragments shorter than   42877 bp
  85% of sequence in fragments shorter than   49984 bp
  90% of sequence in fragments shorter than   60629 bp
  95% of sequence in fragments shorter than   84346 bp

# KasI.SgrAI (GGCGCC+CRCCGGYG) double digest, 245 thousand fragments (230381+15270 sites), 12144 bp average fragment length
   5% of sequence in fragments shorter than    4115 bp
  10% of sequence in fragments shorter than    6767 bp
  15% of sequence in fragments shorter than    9302 bp
  20% of sequence in fragments shorter than   11817 bp
  25% of sequence in fragments shorter than   14496 bp
  30% of sequence in fragments shorter than   17310 bp
  35% of sequence in fragments shorter than   20365 bp
  40% of sequence in fragments shorter than   23706 bp
  45% of sequence in fragments shorter than   27199 bp
  50% of sequence in fragments shorter than   31193 bp
  55% of sequence in fragments shorter than   35630 bp
  60% of sequence in fragments shorter than   40640 bp
  65% of sequence in fragments shorter than   46350 bp
  70% of sequence in fragments shorter than   53022 bp
  75% of sequence in fragments shorter than   60981 bp
  80% of sequence in fragments shorter than   70733 bp
  85% of sequence in fragments shorter than   84529 bp
  90% of sequence in fragments shorter than  104058 bp
  95% of sequence in fragments shorter than  147291 bp

# KasI.NruI (GGCGCC+TCGCGA) double digest, 244 thousand fragments (230381+14872 sites), 12164 bp average fragment length
   5% of sequence in fragments shorter than    4082 bp
  10% of sequence in fragments shorter than    6683 bp
  15% of sequence in fragments shorter than    9165 bp
  20% of sequence in fragments shorter than   11649 bp
  25% of sequence in fragments shorter than   14271 bp
  30% of sequence in fragments shorter than   17084 bp
  35% of sequence in fragments shorter than   20030 bp
  40% of sequence in fragments shorter than   23295 bp
  45% of sequence in fragments shorter than   26746 bp
  50% of sequence in fragments shorter than   30641 bp
  55% of sequence in fragments shorter than   34966 bp
  60% of sequence in fragments shorter than   39784 bp
  65% of sequence in fragments shorter than   45448 bp
  70% of sequence in fragments shorter than   51881 bp
  75% of sequence in fragments shorter than   59622 bp
  80% of sequence in fragments shorter than   69210 bp
  85% of sequence in fragments shorter than   82068 bp
  90% of sequence in fragments shorter than  101281 bp
  95% of sequence in fragments shorter than  141927 bp

# BsrBI.NotI (CCGCTC+GCGGCCGC) double digest, 244 thousand fragments (235523+9795 sites), 12167 bp average fragment length
   5% of sequence in fragments shorter than    4191 bp
  10% of sequence in fragments shorter than    7154 bp
  15% of sequence in fragments shorter than    9922 bp
  20% of sequence in fragments shorter than   12710 bp
  25% of sequence in fragments shorter than   15547 bp
  30% of sequence in fragments shorter than   18505 bp
  35% of sequence in fragments shorter than   21650 bp
  40% of sequence in fragments shorter than   25012 bp
  45% of sequence in fragments shorter than   28724 bp
  50% of sequence in fragments shorter than   32726 bp
  55% of sequence in fragments shorter than   37163 bp
  60% of sequence in fragments shorter than   41908 bp
  65% of sequence in fragments shorter than   47438 bp
  70% of sequence in fragments shorter than   53771 bp
  75% of sequence in fragments shorter than   61634 bp
  80% of sequence in fragments shorter than   71016 bp
  85% of sequence in fragments shorter than   83162 bp
  90% of sequence in fragments shorter than  101345 bp
  95% of sequence in fragments shorter than  136642 bp

# BsrBI.RsrII (CCGCTC+CGGWCCG) double digest, 244 thousand fragments (235523+9764 sites), 12169 bp average fragment length
   5% of sequence in fragments shorter than    4141 bp
  10% of sequence in fragments shorter than    7059 bp
  15% of sequence in fragments shorter than    9788 bp
  20% of sequence in fragments shorter than   12566 bp
  25% of sequence in fragments shorter than   15373 bp
  30% of sequence in fragments shorter than   18276 bp
  35% of sequence in fragments shorter than   21400 bp
  40% of sequence in fragments shorter than   24690 bp
  45% of sequence in fragments shorter than   28392 bp
  50% of sequence in fragments shorter than   32353 bp
  55% of sequence in fragments shorter than   36685 bp
  60% of sequence in fragments shorter than   41469 bp
  65% of sequence in fragments shorter than   46872 bp
  70% of sequence in fragments shorter than   53076 bp
  75% of sequence in fragments shorter than   60818 bp
  80% of sequence in fragments shorter than   69948 bp
  85% of sequence in fragments shorter than   82088 bp
  90% of sequence in fragments shorter than  100063 bp
  95% of sequence in fragments shorter than  134505 bp

# KasI.PvuI (GGCGCC+CGATCG) double digest, 244 thousand fragments (230381+14186 sites), 12198 bp average fragment length
   5% of sequence in fragments shorter than    4046 bp
  10% of sequence in fragments shorter than    6661 bp
  15% of sequence in fragments shorter than    9145 bp
  20% of sequence in fragments shorter than   11614 bp
  25% of sequence in fragments shorter than   14230 bp
  30% of sequence in fragments shorter than   17033 bp
  35% of sequence in fragments shorter than   19984 bp
  40% of sequence in fragments shorter than   23225 bp
  45% of sequence in fragments shorter than   26718 bp
  50% of sequence in fragments shorter than   30601 bp
  55% of sequence in fragments shorter than   34924 bp
  60% of sequence in fragments shorter than   39679 bp
  65% of sequence in fragments shorter than   45264 bp
  70% of sequence in fragments shorter than   51715 bp
  75% of sequence in fragments shorter than   59275 bp
  80% of sequence in fragments shorter than   68530 bp
  85% of sequence in fragments shorter than   81365 bp
  90% of sequence in fragments shorter than  100746 bp
  95% of sequence in fragments shorter than  138885 bp

# ClaI.Hpy99I (ATCGAT+CGWCG) double digest, 244 thousand fragments (86477+157917 sites), 12212 bp average fragment length
   5% of sequence in fragments shorter than    4460 bp
  10% of sequence in fragments shorter than    7142 bp
  15% of sequence in fragments shorter than    9547 bp
  20% of sequence in fragments shorter than   11807 bp
  25% of sequence in fragments shorter than   14095 bp
  30% of sequence in fragments shorter than   16369 bp
  35% of sequence in fragments shorter than   18724 bp
  40% of sequence in fragments shorter than   21188 bp
  45% of sequence in fragments shorter than   23801 bp
  50% of sequence in fragments shorter than   26630 bp
  55% of sequence in fragments shorter than   29647 bp
  60% of sequence in fragments shorter than   32980 bp
  65% of sequence in fragments shorter than   36643 bp
  70% of sequence in fragments shorter than   40942 bp
  75% of sequence in fragments shorter than   45872 bp
  80% of sequence in fragments shorter than   51831 bp
  85% of sequence in fragments shorter than   59861 bp
  90% of sequence in fragments shorter than   71206 bp
  95% of sequence in fragments shorter than   93858 bp

# FseI.KasI (GGCCGGCC+GGCGCC) double digest, 243 thousand fragments (13662+230381 sites), 12224 bp average fragment length
   5% of sequence in fragments shorter than    4091 bp
  10% of sequence in fragments shorter than    6744 bp
  15% of sequence in fragments shorter than    9275 bp
  20% of sequence in fragments shorter than   11811 bp
  25% of sequence in fragments shorter than   14517 bp
  30% of sequence in fragments shorter than   17357 bp
  35% of sequence in fragments shorter than   20444 bp
  40% of sequence in fragments shorter than   23835 bp
  45% of sequence in fragments shorter than   27379 bp
  50% of sequence in fragments shorter than   31463 bp
  55% of sequence in fragments shorter than   36052 bp
  60% of sequence in fragments shorter than   41067 bp
  65% of sequence in fragments shorter than   46984 bp
  70% of sequence in fragments shorter than   53686 bp
  75% of sequence in fragments shorter than   61755 bp
  80% of sequence in fragments shorter than   71561 bp
  85% of sequence in fragments shorter than   85324 bp
  90% of sequence in fragments shorter than  104999 bp
  95% of sequence in fragments shorter than  149046 bp

# AatII.AclI (GACGTC+AACGTT) double digest, 243 thousand fragments (71089+172706 sites), 12243 bp average fragment length
   5% of sequence in fragments shorter than    4202 bp
  10% of sequence in fragments shorter than    6533 bp
  15% of sequence in fragments shorter than    8547 bp
  20% of sequence in fragments shorter than   10413 bp
  25% of sequence in fragments shorter than   12224 bp
  30% of sequence in fragments shorter than   14058 bp
  35% of sequence in fragments shorter than   15918 bp
  40% of sequence in fragments shorter than   17806 bp
  45% of sequence in fragments shorter than   19803 bp
  50% of sequence in fragments shorter than   21946 bp
  55% of sequence in fragments shorter than   24170 bp
  60% of sequence in fragments shorter than   26625 bp
  65% of sequence in fragments shorter than   29364 bp
  70% of sequence in fragments shorter than   32530 bp
  75% of sequence in fragments shorter than   36134 bp
  80% of sequence in fragments shorter than   40519 bp
  85% of sequence in fragments shorter than   46226 bp
  90% of sequence in fragments shorter than   54015 bp
  95% of sequence in fragments shorter than   68235 bp

# SgrAI.SwaI (CRCCGGYG+ATTTAAAT) double digest, 241 thousand fragments (15270+226602 sites), 12333 bp average fragment length
   5% of sequence in fragments shorter than    4241 bp
  10% of sequence in fragments shorter than    6537 bp
  15% of sequence in fragments shorter than    8569 bp
  20% of sequence in fragments shorter than   10460 bp
  25% of sequence in fragments shorter than   12310 bp
  30% of sequence in fragments shorter than   14200 bp
  35% of sequence in fragments shorter than   16169 bp
  40% of sequence in fragments shorter than   18262 bp
  45% of sequence in fragments shorter than   20468 bp
  50% of sequence in fragments shorter than   22834 bp
  55% of sequence in fragments shorter than   25440 bp
  60% of sequence in fragments shorter than   28389 bp
  65% of sequence in fragments shorter than   31794 bp
  70% of sequence in fragments shorter than   35746 bp
  75% of sequence in fragments shorter than   40401 bp
  80% of sequence in fragments shorter than   46490 bp
  85% of sequence in fragments shorter than   54868 bp
  90% of sequence in fragments shorter than   67784 bp
  95% of sequence in fragments shorter than   96809 bp

# NruI.SwaI (TCGCGA+ATTTAAAT) double digest, 241 thousand fragments (14872+226602 sites), 12354 bp average fragment length
   5% of sequence in fragments shorter than    4227 bp
  10% of sequence in fragments shorter than    6508 bp
  15% of sequence in fragments shorter than    8533 bp
  20% of sequence in fragments shorter than   10421 bp
  25% of sequence in fragments shorter than   12274 bp
  30% of sequence in fragments shorter than   14168 bp
  35% of sequence in fragments shorter than   16155 bp
  40% of sequence in fragments shorter than   18261 bp
  45% of sequence in fragments shorter than   20466 bp
  50% of sequence in fragments shorter than   22852 bp
  55% of sequence in fragments shorter than   25508 bp
  60% of sequence in fragments shorter than   28484 bp
  65% of sequence in fragments shorter than   31899 bp
  70% of sequence in fragments shorter than   36049 bp
  75% of sequence in fragments shorter than   40923 bp
  80% of sequence in fragments shorter than   47154 bp
  85% of sequence in fragments shorter than   55997 bp
  90% of sequence in fragments shorter than   70295 bp
  95% of sequence in fragments shorter than  103267 bp

# PvuI.SwaI (CGATCG+ATTTAAAT) double digest, 240 thousand fragments (14186+226602 sites), 12389 bp average fragment length
   5% of sequence in fragments shorter than    4216 bp
  10% of sequence in fragments shorter than    6521 bp
  15% of sequence in fragments shorter than    8566 bp
  20% of sequence in fragments shorter than   10472 bp
  25% of sequence in fragments shorter than   12342 bp
  30% of sequence in fragments shorter than   14254 bp
  35% of sequence in fragments shorter than   16276 bp
  40% of sequence in fragments shorter than   18388 bp
  45% of sequence in fragments shorter than   20623 bp
  50% of sequence in fragments shorter than   22992 bp
  55% of sequence in fragments shorter than   25667 bp
  60% of sequence in fragments shorter than   28715 bp
  65% of sequence in fragments shorter than   32203 bp
  70% of sequence in fragments shorter than   36335 bp
  75% of sequence in fragments shorter than   41286 bp
  80% of sequence in fragments shorter than   47613 bp
  85% of sequence in fragments shorter than   56697 bp
  90% of sequence in fragments shorter than   70966 bp
  95% of sequence in fragments shorter than  102734 bp

# FseI.SwaI (GGCCGGCC+ATTTAAAT) double digest, 240 thousand fragments (13662+226602 sites), 12416 bp average fragment length
   5% of sequence in fragments shorter than    4273 bp
  10% of sequence in fragments shorter than    6578 bp
  15% of sequence in fragments shorter than    8626 bp
  20% of sequence in fragments shorter than   10521 bp
  25% of sequence in fragments shorter than   12372 bp
  30% of sequence in fragments shorter than   14270 bp
  35% of sequence in fragments shorter than   16267 bp
  40% of sequence in fragments shorter than   18361 bp
  45% of sequence in fragments shorter than   20563 bp
  50% of sequence in fragments shorter than   22926 bp
  55% of sequence in fragments shorter than   25530 bp
  60% of sequence in fragments shorter than   28462 bp
  65% of sequence in fragments shorter than   31810 bp
  70% of sequence in fragments shorter than   35741 bp
  75% of sequence in fragments shorter than   40481 bp
  80% of sequence in fragments shorter than   46455 bp
  85% of sequence in fragments shorter than   54824 bp
  90% of sequence in fragments shorter than   67319 bp
  95% of sequence in fragments shorter than   96626 bp

# KasI.NotI (GGCGCC+GCGGCCGC) double digest, 239 thousand fragments (230381+9795 sites), 12421 bp average fragment length
   5% of sequence in fragments shorter than    4207 bp
  10% of sequence in fragments shorter than    6925 bp
  15% of sequence in fragments shorter than    9520 bp
  20% of sequence in fragments shorter than   12118 bp
  25% of sequence in fragments shorter than   14889 bp
  30% of sequence in fragments shorter than   17812 bp
  35% of sequence in fragments shorter than   20981 bp
  40% of sequence in fragments shorter than   24427 bp
  45% of sequence in fragments shorter than   28002 bp
  50% of sequence in fragments shorter than   32144 bp
  55% of sequence in fragments shorter than   36739 bp
  60% of sequence in fragments shorter than   41873 bp
  65% of sequence in fragments shorter than   47854 bp
  70% of sequence in fragments shorter than   54587 bp
  75% of sequence in fragments shorter than   62900 bp
  80% of sequence in fragments shorter than   73047 bp
  85% of sequence in fragments shorter than   86921 bp
  90% of sequence in fragments shorter than  107007 bp
  95% of sequence in fragments shorter than  153016 bp

# KasI.RsrII (GGCGCC+CGGWCCG) double digest, 239 thousand fragments (230381+9764 sites), 12423 bp average fragment length
   5% of sequence in fragments shorter than    4167 bp
  10% of sequence in fragments shorter than    6849 bp
  15% of sequence in fragments shorter than    9418 bp
  20% of sequence in fragments shorter than   11976 bp
  25% of sequence in fragments shorter than   14718 bp
  30% of sequence in fragments shorter than   17583 bp
  35% of sequence in fragments shorter than   20696 bp
  40% of sequence in fragments shorter than   24103 bp
  45% of sequence in fragments shorter than   27607 bp
  50% of sequence in fragments shorter than   31720 bp
  55% of sequence in fragments shorter than   36302 bp
  60% of sequence in fragments shorter than   41334 bp
  65% of sequence in fragments shorter than   47206 bp
  70% of sequence in fragments shorter than   53912 bp
  75% of sequence in fragments shorter than   62046 bp
  80% of sequence in fragments shorter than   71899 bp
  85% of sequence in fragments shorter than   85734 bp
  90% of sequence in fragments shorter than  105247 bp
  95% of sequence in fragments shorter than  149113 bp

# AscI.BsrBI (GGCGCGCC+CCGCTC) double digest, 239 thousand fragments (4751+235523 sites), 12423 bp average fragment length
   5% of sequence in fragments shorter than    4227 bp
  10% of sequence in fragments shorter than    7192 bp
  15% of sequence in fragments shorter than    9974 bp
  20% of sequence in fragments shorter than   12753 bp
  25% of sequence in fragments shorter than   15595 bp
  30% of sequence in fragments shorter than   18570 bp
  35% of sequence in fragments shorter than   21715 bp
  40% of sequence in fragments shorter than   25088 bp
  45% of sequence in fragments shorter than   28807 bp
  50% of sequence in fragments shorter than   32832 bp
  55% of sequence in fragments shorter than   37268 bp
  60% of sequence in fragments shorter than   41999 bp
  65% of sequence in fragments shorter than   47518 bp
  70% of sequence in fragments shorter than   53817 bp
  75% of sequence in fragments shorter than   61716 bp
  80% of sequence in fragments shorter than   71065 bp
  85% of sequence in fragments shorter than   83187 bp
  90% of sequence in fragments shorter than  101385 bp
  95% of sequence in fragments shorter than  137100 bp

# AclI.SacII (AACGTT+CCGCGG) double digest, 239 thousand fragments (172706+66901 sites), 12457 bp average fragment length
   5% of sequence in fragments shorter than    4626 bp
  10% of sequence in fragments shorter than    7353 bp
  15% of sequence in fragments shorter than    9690 bp
  20% of sequence in fragments shorter than   11844 bp
  25% of sequence in fragments shorter than   13988 bp
  30% of sequence in fragments shorter than   16119 bp
  35% of sequence in fragments shorter than   18285 bp
  40% of sequence in fragments shorter than   20533 bp
  45% of sequence in fragments shorter than   22831 bp
  50% of sequence in fragments shorter than   25241 bp
  55% of sequence in fragments shorter than   27872 bp
  60% of sequence in fragments shorter than   30776 bp
  65% of sequence in fragments shorter than   34038 bp
  70% of sequence in fragments shorter than   37785 bp
  75% of sequence in fragments shorter than   42026 bp
  80% of sequence in fragments shorter than   47025 bp
  85% of sequence in fragments shorter than   53673 bp
  90% of sequence in fragments shorter than   63111 bp
  95% of sequence in fragments shorter than   79637 bp

# NotI.SwaI (GCGGCCGC+ATTTAAAT) double digest, 236 thousand fragments (9795+226602 sites), 12619 bp average fragment length
   5% of sequence in fragments shorter than    4334 bp
  10% of sequence in fragments shorter than    6683 bp
  15% of sequence in fragments shorter than    8766 bp
  20% of sequence in fragments shorter than   10707 bp
  25% of sequence in fragments shorter than   12627 bp
  30% of sequence in fragments shorter than   14572 bp
  35% of sequence in fragments shorter than   16628 bp
  40% of sequence in fragments shorter than   18778 bp
  45% of sequence in fragments shorter than   21077 bp
  50% of sequence in fragments shorter than   23531 bp
  55% of sequence in fragments shorter than   26272 bp
  60% of sequence in fragments shorter than   29380 bp
  65% of sequence in fragments shorter than   32950 bp
  70% of sequence in fragments shorter than   37135 bp
  75% of sequence in fragments shorter than   42160 bp
  80% of sequence in fragments shorter than   48793 bp
  85% of sequence in fragments shorter than   57980 bp
  90% of sequence in fragments shorter than   72981 bp
  95% of sequence in fragments shorter than  106837 bp

# RsrII.SwaI (CGGWCCG+ATTTAAAT) double digest, 236 thousand fragments (9764+226602 sites), 12621 bp average fragment length
   5% of sequence in fragments shorter than    4319 bp
  10% of sequence in fragments shorter than    6654 bp
  15% of sequence in fragments shorter than    8726 bp
  20% of sequence in fragments shorter than   10656 bp
  25% of sequence in fragments shorter than   12570 bp
  30% of sequence in fragments shorter than   14510 bp
  35% of sequence in fragments shorter than   16575 bp
  40% of sequence in fragments shorter than   18709 bp
  45% of sequence in fragments shorter than   21002 bp
  50% of sequence in fragments shorter than   23438 bp
  55% of sequence in fragments shorter than   26184 bp
  60% of sequence in fragments shorter than   29298 bp
  65% of sequence in fragments shorter than   32850 bp
  70% of sequence in fragments shorter than   37035 bp
  75% of sequence in fragments shorter than   42117 bp
  80% of sequence in fragments shorter than   48651 bp
  85% of sequence in fragments shorter than   57766 bp
  90% of sequence in fragments shorter than   72246 bp
  95% of sequence in fragments shorter than  105882 bp

# AscI.KasI (GGCGCGCC+GGCGCC) double digest, 234 thousand fragments (4751+230381 sites), 12687 bp average fragment length
   5% of sequence in fragments shorter than    4261 bp
  10% of sequence in fragments shorter than    6988 bp
  15% of sequence in fragments shorter than    9598 bp
  20% of sequence in fragments shorter than   12215 bp
  25% of sequence in fragments shorter than   14994 bp
  30% of sequence in fragments shorter than   17923 bp
  35% of sequence in fragments shorter than   21081 bp
  40% of sequence in fragments shorter than   24553 bp
  45% of sequence in fragments shorter than   28157 bp
  50% of sequence in fragments shorter than   32338 bp
  55% of sequence in fragments shorter than   36942 bp
  60% of sequence in fragments shorter than   42116 bp
  65% of sequence in fragments shorter than   48081 bp
  70% of sequence in fragments shorter than   54842 bp
  75% of sequence in fragments shorter than   63230 bp
  80% of sequence in fragments shorter than   73412 bp
  85% of sequence in fragments shorter than   87210 bp
  90% of sequence in fragments shorter than  107191 bp
  95% of sequence in fragments shorter than  153054 bp

# AclI.PspXI (AACGTT+VCTCGAGB) double digest, 231 thousand fragments (172706+59193 sites), 12872 bp average fragment length
   5% of sequence in fragments shorter than    4428 bp
  10% of sequence in fragments shorter than    6873 bp
  15% of sequence in fragments shorter than    8962 bp
  20% of sequence in fragments shorter than   10906 bp
  25% of sequence in fragments shorter than   12789 bp
  30% of sequence in fragments shorter than   14684 bp
  35% of sequence in fragments shorter than   16577 bp
  40% of sequence in fragments shorter than   18558 bp
  45% of sequence in fragments shorter than   20652 bp
  50% of sequence in fragments shorter than   22858 bp
  55% of sequence in fragments shorter than   25165 bp
  60% of sequence in fragments shorter than   27801 bp
  65% of sequence in fragments shorter than   30612 bp
  70% of sequence in fragments shorter than   33922 bp
  75% of sequence in fragments shorter than   37817 bp
  80% of sequence in fragments shorter than   42350 bp
  85% of sequence in fragments shorter than   48234 bp
  90% of sequence in fragments shorter than   56214 bp
  95% of sequence in fragments shorter than   71295 bp

# AscI.SwaI (GGCGCGCC+ATTTAAAT) double digest, 231 thousand fragments (4751+226602 sites), 12895 bp average fragment length
   5% of sequence in fragments shorter than    4394 bp
  10% of sequence in fragments shorter than    6779 bp
  15% of sequence in fragments shorter than    8894 bp
  20% of sequence in fragments shorter than   10866 bp
  25% of sequence in fragments shorter than   12837 bp
  30% of sequence in fragments shorter than   14824 bp
  35% of sequence in fragments shorter than   16949 bp
  40% of sequence in fragments shorter than   19182 bp
  45% of sequence in fragments shorter than   21579 bp
  50% of sequence in fragments shorter than   24112 bp
  55% of sequence in fragments shorter than   26961 bp
  60% of sequence in fragments shorter than   30238 bp
  65% of sequence in fragments shorter than   33980 bp
  70% of sequence in fragments shorter than   38517 bp
  75% of sequence in fragments shorter than   43926 bp
  80% of sequence in fragments shorter than   51163 bp
  85% of sequence in fragments shorter than   61379 bp
  90% of sequence in fragments shorter than   78737 bp
  95% of sequence in fragments shorter than  120990 bp

# AatII.PacI (GACGTC+TTAATTAA) double digest, 230 thousand fragments (71089+159455 sites), 12943 bp average fragment length
   5% of sequence in fragments shorter than    4769 bp
  10% of sequence in fragments shorter than    7231 bp
  15% of sequence in fragments shorter than    9355 bp
  20% of sequence in fragments shorter than   11325 bp
  25% of sequence in fragments shorter than   13255 bp
  30% of sequence in fragments shorter than   15223 bp
  35% of sequence in fragments shorter than   17199 bp
  40% of sequence in fragments shorter than   19239 bp
  45% of sequence in fragments shorter than   21428 bp
  50% of sequence in fragments shorter than   23713 bp
  55% of sequence in fragments shorter than   26159 bp
  60% of sequence in fragments shorter than   28824 bp
  65% of sequence in fragments shorter than   31729 bp
  70% of sequence in fragments shorter than   35008 bp
  75% of sequence in fragments shorter than   39016 bp
  80% of sequence in fragments shorter than   43705 bp
  85% of sequence in fragments shorter than   50119 bp
  90% of sequence in fragments shorter than   59320 bp
  95% of sequence in fragments shorter than   78903 bp

# AatII.Hpy99I (GACGTC+CGWCG) double digest, 228 thousand fragments (71089+157917 sites), 13033 bp average fragment length
   5% of sequence in fragments shorter than    4888 bp
  10% of sequence in fragments shorter than    8081 bp
  15% of sequence in fragments shorter than   11045 bp
  20% of sequence in fragments shorter than   13948 bp
  25% of sequence in fragments shorter than   16876 bp
  30% of sequence in fragments shorter than   19926 bp
  35% of sequence in fragments shorter than   23114 bp
  40% of sequence in fragments shorter than   26486 bp
  45% of sequence in fragments shorter than   30150 bp
  50% of sequence in fragments shorter than   34009 bp
  55% of sequence in fragments shorter than   38312 bp
  60% of sequence in fragments shorter than   43042 bp
  65% of sequence in fragments shorter than   48570 bp
  70% of sequence in fragments shorter than   54826 bp
  75% of sequence in fragments shorter than   62277 bp
  80% of sequence in fragments shorter than   71199 bp
  85% of sequence in fragments shorter than   83179 bp
  90% of sequence in fragments shorter than  100527 bp
  95% of sequence in fragments shorter than  133801 bp

# PacI.SacII (TTAATTAA+CCGCGG) double digest, 226 thousand fragments (159455+66901 sites), 13179 bp average fragment length
   5% of sequence in fragments shorter than    5291 bp
  10% of sequence in fragments shorter than    8145 bp
  15% of sequence in fragments shorter than   10588 bp
  20% of sequence in fragments shorter than   12903 bp
  25% of sequence in fragments shorter than   15169 bp
  30% of sequence in fragments shorter than   17438 bp
  35% of sequence in fragments shorter than   19745 bp
  40% of sequence in fragments shorter than   22206 bp
  45% of sequence in fragments shorter than   24710 bp
  50% of sequence in fragments shorter than   27387 bp
  55% of sequence in fragments shorter than   30226 bp
  60% of sequence in fragments shorter than   33365 bp
  65% of sequence in fragments shorter than   36989 bp
  70% of sequence in fragments shorter than   40998 bp
  75% of sequence in fragments shorter than   45661 bp
  80% of sequence in fragments shorter than   51615 bp
  85% of sequence in fragments shorter than   59407 bp
  90% of sequence in fragments shorter than   70701 bp
  95% of sequence in fragments shorter than   95229 bp

# AclI.AgeI (AACGTT+ACCGGT) double digest, 225 thousand fragments (172706+53386 sites), 13203 bp average fragment length
   5% of sequence in fragments shorter than    4553 bp
  10% of sequence in fragments shorter than    7078 bp
  15% of sequence in fragments shorter than    9249 bp
  20% of sequence in fragments shorter than   11253 bp
  25% of sequence in fragments shorter than   13186 bp
  30% of sequence in fragments shorter than   15148 bp
  35% of sequence in fragments shorter than   17125 bp
  40% of sequence in fragments shorter than   19146 bp
  45% of sequence in fragments shorter than   21312 bp
  50% of sequence in fragments shorter than   23573 bp
  55% of sequence in fragments shorter than   25954 bp
  60% of sequence in fragments shorter than   28568 bp
  65% of sequence in fragments shorter than   31490 bp
  70% of sequence in fragments shorter than   34748 bp
  75% of sequence in fragments shorter than   38586 bp
  80% of sequence in fragments shorter than   43182 bp
  85% of sequence in fragments shorter than   49075 bp
  90% of sequence in fragments shorter than   57557 bp
  95% of sequence in fragments shorter than   72296 bp

# Hpy99I.SacII (CGWCG+CCGCGG) double digest, 224 thousand fragments (157917+66901 sites), 13275 bp average fragment length
   5% of sequence in fragments shorter than    5523 bp
  10% of sequence in fragments shorter than    9610 bp
  15% of sequence in fragments shorter than   13491 bp
  20% of sequence in fragments shorter than   17392 bp
  25% of sequence in fragments shorter than   21411 bp
  30% of sequence in fragments shorter than   25666 bp
  35% of sequence in fragments shorter than   30167 bp
  40% of sequence in fragments shorter than   34925 bp
  45% of sequence in fragments shorter than   40032 bp
  50% of sequence in fragments shorter than   45649 bp
  55% of sequence in fragments shorter than   51810 bp
  60% of sequence in fragments shorter than   58841 bp
  65% of sequence in fragments shorter than   66961 bp
  70% of sequence in fragments shorter than   76117 bp
  75% of sequence in fragments shorter than   87318 bp
  80% of sequence in fragments shorter than  101516 bp
  85% of sequence in fragments shorter than  120670 bp
  90% of sequence in fragments shorter than  147426 bp
  95% of sequence in fragments shorter than  205686 bp

# ClaI.SnaBI (ATCGAT+TACGTA) double digest, 218 thousand fragments (86477+132299 sites), 13643 bp average fragment length
   5% of sequence in fragments shorter than    4774 bp
  10% of sequence in fragments shorter than    7369 bp
  15% of sequence in fragments shorter than    9572 bp
  20% of sequence in fragments shorter than   11626 bp
  25% of sequence in fragments shorter than   13640 bp
  30% of sequence in fragments shorter than   15678 bp
  35% of sequence in fragments shorter than   17697 bp
  40% of sequence in fragments shorter than   19864 bp
  45% of sequence in fragments shorter than   22105 bp
  50% of sequence in fragments shorter than   24516 bp
  55% of sequence in fragments shorter than   27101 bp
  60% of sequence in fragments shorter than   29970 bp
  65% of sequence in fragments shorter than   33139 bp
  70% of sequence in fragments shorter than   36686 bp
  75% of sequence in fragments shorter than   41035 bp
  80% of sequence in fragments shorter than   46299 bp
  85% of sequence in fragments shorter than   52779 bp
  90% of sequence in fragments shorter than   63019 bp
  95% of sequence in fragments shorter than   83199 bp

# PacI.PspXI (TTAATTAA+VCTCGAGB) double digest, 218 thousand fragments (159455+59193 sites), 13646 bp average fragment length
   5% of sequence in fragments shorter than    5033 bp
  10% of sequence in fragments shorter than    7603 bp
  15% of sequence in fragments shorter than    9828 bp
  20% of sequence in fragments shorter than   11925 bp
  25% of sequence in fragments shorter than   13967 bp
  30% of sequence in fragments shorter than   16009 bp
  35% of sequence in fragments shorter than   18006 bp
  40% of sequence in fragments shorter than   20149 bp
  45% of sequence in fragments shorter than   22409 bp
  50% of sequence in fragments shorter than   24766 bp
  55% of sequence in fragments shorter than   27322 bp
  60% of sequence in fragments shorter than   30087 bp
  65% of sequence in fragments shorter than   33260 bp
  70% of sequence in fragments shorter than   36775 bp
  75% of sequence in fragments shorter than   40816 bp
  80% of sequence in fragments shorter than   45870 bp
  85% of sequence in fragments shorter than   52522 bp
  90% of sequence in fragments shorter than   62112 bp
  95% of sequence in fragments shorter than   83395 bp

# Hpy99I.PspXI (CGWCG+VCTCGAGB) double digest, 216 thousand fragments (157917+59193 sites), 13748 bp average fragment length
   5% of sequence in fragments shorter than    4876 bp
  10% of sequence in fragments shorter than    8114 bp
  15% of sequence in fragments shorter than   11144 bp
  20% of sequence in fragments shorter than   14196 bp
  25% of sequence in fragments shorter than   17247 bp
  30% of sequence in fragments shorter than   20451 bp
  35% of sequence in fragments shorter than   23831 bp
  40% of sequence in fragments shorter than   27394 bp
  45% of sequence in fragments shorter than   31280 bp
  50% of sequence in fragments shorter than   35528 bp
  55% of sequence in fragments shorter than   40158 bp
  60% of sequence in fragments shorter than   45326 bp
  65% of sequence in fragments shorter than   51332 bp
  70% of sequence in fragments shorter than   58083 bp
  75% of sequence in fragments shorter than   66612 bp
  80% of sequence in fragments shorter than   76624 bp
  85% of sequence in fragments shorter than   89757 bp
  90% of sequence in fragments shorter than  109017 bp
  95% of sequence in fragments shorter than  147573 bp

# AclI.PmeI (AACGTT+GTTTAAAC) double digest, 214 thousand fragments (172706+42094 sites), 13899 bp average fragment length
   5% of sequence in fragments shorter than    4784 bp
  10% of sequence in fragments shorter than    7427 bp
  15% of sequence in fragments shorter than    9707 bp
  20% of sequence in fragments shorter than   11806 bp
  25% of sequence in fragments shorter than   13871 bp
  30% of sequence in fragments shorter than   15926 bp
  35% of sequence in fragments shorter than   18022 bp
  40% of sequence in fragments shorter than   20164 bp
  45% of sequence in fragments shorter than   22405 bp
  50% of sequence in fragments shorter than   24772 bp
  55% of sequence in fragments shorter than   27328 bp
  60% of sequence in fragments shorter than   30155 bp
  65% of sequence in fragments shorter than   33288 bp
  70% of sequence in fragments shorter than   36922 bp
  75% of sequence in fragments shorter than   41227 bp
  80% of sequence in fragments shorter than   46322 bp
  85% of sequence in fragments shorter than   52878 bp
  90% of sequence in fragments shorter than   62277 bp
  95% of sequence in fragments shorter than   79657 bp

# AgeI.PacI (ACCGGT+TTAATTAA) double digest, 212 thousand fragments (53386+159455 sites), 14018 bp average fragment length
   5% of sequence in fragments shorter than    5152 bp
  10% of sequence in fragments shorter than    7777 bp
  15% of sequence in fragments shorter than   10028 bp
  20% of sequence in fragments shorter than   12162 bp
  25% of sequence in fragments shorter than   14236 bp
  30% of sequence in fragments shorter than   16364 bp
  35% of sequence in fragments shorter than   18482 bp
  40% of sequence in fragments shorter than   20720 bp
  45% of sequence in fragments shorter than   23037 bp
  50% of sequence in fragments shorter than   25527 bp
  55% of sequence in fragments shorter than   28196 bp
  60% of sequence in fragments shorter than   31050 bp
  65% of sequence in fragments shorter than   34268 bp
  70% of sequence in fragments shorter than   38084 bp
  75% of sequence in fragments shorter than   42405 bp
  80% of sequence in fragments shorter than   47693 bp
  85% of sequence in fragments shorter than   54844 bp
  90% of sequence in fragments shorter than   65656 bp
  95% of sequence in fragments shorter than   88065 bp

# ClaI.NaeI (ATCGAT+GCCGGC) double digest, 211 thousand fragments (86477+125692 sites), 14064 bp average fragment length
   5% of sequence in fragments shorter than    5055 bp
  10% of sequence in fragments shorter than    8127 bp
  15% of sequence in fragments shorter than   10885 bp
  20% of sequence in fragments shorter than   13482 bp
  25% of sequence in fragments shorter than   16148 bp
  30% of sequence in fragments shorter than   18856 bp
  35% of sequence in fragments shorter than   21638 bp
  40% of sequence in fragments shorter than   24534 bp
  45% of sequence in fragments shorter than   27602 bp
  50% of sequence in fragments shorter than   30878 bp
  55% of sequence in fragments shorter than   34435 bp
  60% of sequence in fragments shorter than   38394 bp
  65% of sequence in fragments shorter than   42810 bp
  70% of sequence in fragments shorter than   47761 bp
  75% of sequence in fragments shorter than   53580 bp
  80% of sequence in fragments shorter than   60943 bp
  85% of sequence in fragments shorter than   70674 bp
  90% of sequence in fragments shorter than   85465 bp
  95% of sequence in fragments shorter than  115299 bp

# AgeI.Hpy99I (ACCGGT+CGWCG) double digest, 210 thousand fragments (53386+157917 sites), 14127 bp average fragment length
   5% of sequence in fragments shorter than    5109 bp
  10% of sequence in fragments shorter than    8466 bp
  15% of sequence in fragments shorter than   11519 bp
  20% of sequence in fragments shorter than   14545 bp
  25% of sequence in fragments shorter than   17532 bp
  30% of sequence in fragments shorter than   20615 bp
  35% of sequence in fragments shorter than   23910 bp
  40% of sequence in fragments shorter than   27319 bp
  45% of sequence in fragments shorter than   31014 bp
  50% of sequence in fragments shorter than   34995 bp
  55% of sequence in fragments shorter than   39482 bp
  60% of sequence in fragments shorter than   44438 bp
  65% of sequence in fragments shorter than   49825 bp
  70% of sequence in fragments shorter than   55904 bp
  75% of sequence in fragments shorter than   63486 bp
  80% of sequence in fragments shorter than   72659 bp
  85% of sequence in fragments shorter than   85368 bp
  90% of sequence in fragments shorter than  103750 bp
  95% of sequence in fragments shorter than  138730 bp

# ClaI.XhoI (ATCGAT+CTCGAG) double digest, 207 thousand fragments (86477+121308 sites), 14364 bp average fragment length
   5% of sequence in fragments shorter than    5020 bp
  10% of sequence in fragments shorter than    7665 bp
  15% of sequence in fragments shorter than    9938 bp
  20% of sequence in fragments shorter than   12080 bp
  25% of sequence in fragments shorter than   14222 bp
  30% of sequence in fragments shorter than   16324 bp
  35% of sequence in fragments shorter than   18502 bp
  40% of sequence in fragments shorter than   20792 bp
  45% of sequence in fragments shorter than   23192 bp
  50% of sequence in fragments shorter than   25743 bp
  55% of sequence in fragments shorter than   28417 bp
  60% of sequence in fragments shorter than   31460 bp
  65% of sequence in fragments shorter than   34908 bp
  70% of sequence in fragments shorter than   38720 bp
  75% of sequence in fragments shorter than   43154 bp
  80% of sequence in fragments shorter than   48608 bp
  85% of sequence in fragments shorter than   55823 bp
  90% of sequence in fragments shorter than   66089 bp
  95% of sequence in fragments shorter than   87539 bp

# AclI.SalI (AACGTT+GTCGAC) double digest, 203 thousand fragments (172706+31396 sites), 14628 bp average fragment length
   5% of sequence in fragments shorter than    5060 bp
  10% of sequence in fragments shorter than    7861 bp
  15% of sequence in fragments shorter than   10233 bp
  20% of sequence in fragments shorter than   12439 bp
  25% of sequence in fragments shorter than   14609 bp
  30% of sequence in fragments shorter than   16764 bp
  35% of sequence in fragments shorter than   18963 bp
  40% of sequence in fragments shorter than   21239 bp
  45% of sequence in fragments shorter than   23579 bp
  50% of sequence in fragments shorter than   26011 bp
  55% of sequence in fragments shorter than   28691 bp
  60% of sequence in fragments shorter than   31660 bp
  65% of sequence in fragments shorter than   34861 bp
  70% of sequence in fragments shorter than   38575 bp
  75% of sequence in fragments shorter than   42834 bp
  80% of sequence in fragments shorter than   48029 bp
  85% of sequence in fragments shorter than   54565 bp
  90% of sequence in fragments shorter than   63902 bp
  95% of sequence in fragments shorter than   80332 bp

# AatII.SnaBI (GACGTC+TACGTA) double digest, 203 thousand fragments (71089+132299 sites), 14676 bp average fragment length
   5% of sequence in fragments shorter than    5198 bp
  10% of sequence in fragments shorter than    7973 bp
  15% of sequence in fragments shorter than   10409 bp
  20% of sequence in fragments shorter than   12640 bp
  25% of sequence in fragments shorter than   14842 bp
  30% of sequence in fragments shorter than   17037 bp
  35% of sequence in fragments shorter than   19254 bp
  40% of sequence in fragments shorter than   21557 bp
  45% of sequence in fragments shorter than   23976 bp
  50% of sequence in fragments shorter than   26551 bp
  55% of sequence in fragments shorter than   29243 bp
  60% of sequence in fragments shorter than   32204 bp
  65% of sequence in fragments shorter than   35557 bp
  70% of sequence in fragments shorter than   39374 bp
  75% of sequence in fragments shorter than   43757 bp
  80% of sequence in fragments shorter than   48966 bp
  85% of sequence in fragments shorter than   55789 bp
  90% of sequence in fragments shorter than   65896 bp
  95% of sequence in fragments shorter than   85401 bp

# PacI.PmeI (TTAATTAA+GTTTAAAC) double digest, 201 thousand fragments (159455+42094 sites), 14806 bp average fragment length
   5% of sequence in fragments shorter than    5345 bp
  10% of sequence in fragments shorter than    8140 bp
  15% of sequence in fragments shorter than   10551 bp
  20% of sequence in fragments shorter than   12865 bp
  25% of sequence in fragments shorter than   15222 bp
  30% of sequence in fragments shorter than   17510 bp
  35% of sequence in fragments shorter than   19921 bp
  40% of sequence in fragments shorter than   22437 bp
  45% of sequence in fragments shorter than   25092 bp
  50% of sequence in fragments shorter than   27944 bp
  55% of sequence in fragments shorter than   31055 bp
  60% of sequence in fragments shorter than   34532 bp
  65% of sequence in fragments shorter than   38514 bp
  70% of sequence in fragments shorter than   43062 bp
  75% of sequence in fragments shorter than   48698 bp
  80% of sequence in fragments shorter than   56015 bp
  85% of sequence in fragments shorter than   66319 bp
  90% of sequence in fragments shorter than   81880 bp
  95% of sequence in fragments shorter than  116905 bp

# Hpy99I.PmeI (CGWCG+GTTTAAAC) double digest, 199 thousand fragments (157917+42094 sites), 14927 bp average fragment length
   5% of sequence in fragments shorter than    5535 bp
  10% of sequence in fragments shorter than    8965 bp
  15% of sequence in fragments shorter than   12015 bp
  20% of sequence in fragments shorter than   15025 bp
  25% of sequence in fragments shorter than   18020 bp
  30% of sequence in fragments shorter than   21023 bp
  35% of sequence in fragments shorter than   24170 bp
  40% of sequence in fragments shorter than   27456 bp
  45% of sequence in fragments shorter than   30929 bp
  50% of sequence in fragments shorter than   34677 bp
  55% of sequence in fragments shorter than   38725 bp
  60% of sequence in fragments shorter than   43135 bp
  65% of sequence in fragments shorter than   48119 bp
  70% of sequence in fragments shorter than   53721 bp
  75% of sequence in fragments shorter than   60459 bp
  80% of sequence in fragments shorter than   68631 bp
  85% of sequence in fragments shorter than   79623 bp
  90% of sequence in fragments shorter than   94880 bp
  95% of sequence in fragments shorter than  124199 bp

# SacII.SnaBI (CCGCGG+TACGTA) double digest, 198 thousand fragments (66901+132299 sites), 14983 bp average fragment length
   5% of sequence in fragments shorter than    5883 bp
  10% of sequence in fragments shorter than    9212 bp
  15% of sequence in fragments shorter than   12075 bp
  20% of sequence in fragments shorter than   14748 bp
  25% of sequence in fragments shorter than   17408 bp
  30% of sequence in fragments shorter than   20049 bp
  35% of sequence in fragments shorter than   22796 bp
  40% of sequence in fragments shorter than   25605 bp
  45% of sequence in fragments shorter than   28483 bp
  50% of sequence in fragments shorter than   31519 bp
  55% of sequence in fragments shorter than   34793 bp
  60% of sequence in fragments shorter than   38417 bp
  65% of sequence in fragments shorter than   42413 bp
  70% of sequence in fragments shorter than   46985 bp
  75% of sequence in fragments shorter than   52346 bp
  80% of sequence in fragments shorter than   58907 bp
  85% of sequence in fragments shorter than   67792 bp
  90% of sequence in fragments shorter than   80716 bp
  95% of sequence in fragments shorter than  106521 bp

# AatII.NaeI (GACGTC+GCCGGC) double digest, 196 thousand fragments (71089+125692 sites), 15167 bp average fragment length
   5% of sequence in fragments shorter than    5237 bp
  10% of sequence in fragments shorter than    8825 bp
  15% of sequence in fragments shorter than   12251 bp
  20% of sequence in fragments shorter than   15681 bp
  25% of sequence in fragments shorter than   19216 bp
  30% of sequence in fragments shorter than   22966 bp
  35% of sequence in fragments shorter than   26866 bp
  40% of sequence in fragments shorter than   30990 bp
  45% of sequence in fragments shorter than   35440 bp
  50% of sequence in fragments shorter than   40437 bp
  55% of sequence in fragments shorter than   45991 bp
  60% of sequence in fragments shorter than   52128 bp
  65% of sequence in fragments shorter than   59106 bp
  70% of sequence in fragments shorter than   67092 bp
  75% of sequence in fragments shorter than   76899 bp
  80% of sequence in fragments shorter than   88706 bp
  85% of sequence in fragments shorter than  104184 bp
  90% of sequence in fragments shorter than  126863 bp
  95% of sequence in fragments shorter than  175916 bp

# AclI.MluI (AACGTT+ACGCGT) double digest, 193 thousand fragments (172706+21415 sites), 15382 bp average fragment length
   5% of sequence in fragments shorter than    5330 bp
  10% of sequence in fragments shorter than    8293 bp
  15% of sequence in fragments shorter than   10815 bp
  20% of sequence in fragments shorter than   13160 bp
  25% of sequence in fragments shorter than   15473 bp
  30% of sequence in fragments shorter than   17770 bp
  35% of sequence in fragments shorter than   20097 bp
  40% of sequence in fragments shorter than   22502 bp
  45% of sequence in fragments shorter than   24948 bp
  50% of sequence in fragments shorter than   27581 bp
  55% of sequence in fragments shorter than   30450 bp
  60% of sequence in fragments shorter than   33557 bp
  65% of sequence in fragments shorter than   37041 bp
  70% of sequence in fragments shorter than   40984 bp
  75% of sequence in fragments shorter than   45491 bp
  80% of sequence in fragments shorter than   51039 bp
  85% of sequence in fragments shorter than   58039 bp
  90% of sequence in fragments shorter than   68322 bp
  95% of sequence in fragments shorter than   86121 bp

# NaeI.SacII (GCCGGC+CCGCGG) double digest, 192 thousand fragments (125692+66901 sites), 15491 bp average fragment length
   5% of sequence in fragments shorter than    6363 bp
  10% of sequence in fragments shorter than   11453 bp
  15% of sequence in fragments shorter than   16485 bp
  20% of sequence in fragments shorter than   21845 bp
  25% of sequence in fragments shorter than   27542 bp
  30% of sequence in fragments shorter than   33607 bp
  35% of sequence in fragments shorter than   40120 bp
  40% of sequence in fragments shorter than   47337 bp
  45% of sequence in fragments shorter than   55086 bp
  50% of sequence in fragments shorter than   63826 bp
  55% of sequence in fragments shorter than   73992 bp
  60% of sequence in fragments shorter than   85395 bp
  65% of sequence in fragments shorter than   98828 bp
  70% of sequence in fragments shorter than  113633 bp
  75% of sequence in fragments shorter than  132293 bp
  80% of sequence in fragments shorter than  157588 bp
  85% of sequence in fragments shorter than  189567 bp
  90% of sequence in fragments shorter than  243910 bp
  95% of sequence in fragments shorter than  373544 bp

# AatII.XhoI (GACGTC+CTCGAG) double digest, 192 thousand fragments (71089+121308 sites), 15516 bp average fragment length
   5% of sequence in fragments shorter than    5300 bp
  10% of sequence in fragments shorter than    8171 bp
  15% of sequence in fragments shorter than   10748 bp
  20% of sequence in fragments shorter than   13208 bp
  25% of sequence in fragments shorter than   15743 bp
  30% of sequence in fragments shorter than   18307 bp
  35% of sequence in fragments shorter than   20997 bp
  40% of sequence in fragments shorter than   23853 bp
  45% of sequence in fragments shorter than   26827 bp
  50% of sequence in fragments shorter than   29988 bp
  55% of sequence in fragments shorter than   33555 bp
  60% of sequence in fragments shorter than   37490 bp
  65% of sequence in fragments shorter than   42047 bp
  70% of sequence in fragments shorter than   47131 bp
  75% of sequence in fragments shorter than   53147 bp
  80% of sequence in fragments shorter than   60709 bp
  85% of sequence in fragments shorter than   70487 bp
  90% of sequence in fragments shorter than   85128 bp
  95% of sequence in fragments shorter than  113772 bp

# PspXI.SnaBI (VCTCGAGB+TACGTA) double digest, 191 thousand fragments (59193+132299 sites), 15589 bp average fragment length
   5% of sequence in fragments shorter than    5536 bp
  10% of sequence in fragments shorter than    8483 bp
  15% of sequence in fragments shorter than   11016 bp
  20% of sequence in fragments shorter than   13365 bp
  25% of sequence in fragments shorter than   15655 bp
  30% of sequence in fragments shorter than   17947 bp
  35% of sequence in fragments shorter than   20302 bp
  40% of sequence in fragments shorter than   22745 bp
  45% of sequence in fragments shorter than   25305 bp
  50% of sequence in fragments shorter than   27932 bp
  55% of sequence in fragments shorter than   30816 bp
  60% of sequence in fragments shorter than   33895 bp
  65% of sequence in fragments shorter than   37389 bp
  70% of sequence in fragments shorter than   41369 bp
  75% of sequence in fragments shorter than   46055 bp
  80% of sequence in fragments shorter than   51547 bp
  85% of sequence in fragments shorter than   58792 bp
  90% of sequence in fragments shorter than   69632 bp
  95% of sequence in fragments shorter than   91969 bp

# PacI.SalI (TTAATTAA+GTCGAC) double digest, 190 thousand fragments (159455+31396 sites), 15637 bp average fragment length
   5% of sequence in fragments shorter than    5747 bp
  10% of sequence in fragments shorter than    8716 bp
  15% of sequence in fragments shorter than   11289 bp
  20% of sequence in fragments shorter than   13724 bp
  25% of sequence in fragments shorter than   16098 bp
  30% of sequence in fragments shorter than   18488 bp
  35% of sequence in fragments shorter than   20926 bp
  40% of sequence in fragments shorter than   23526 bp
  45% of sequence in fragments shorter than   26195 bp
  50% of sequence in fragments shorter than   29007 bp
  55% of sequence in fragments shorter than   32145 bp
  60% of sequence in fragments shorter than   35590 bp
  65% of sequence in fragments shorter than   39413 bp
  70% of sequence in fragments shorter than   43647 bp
  75% of sequence in fragments shorter than   48884 bp
  80% of sequence in fragments shorter than   55249 bp
  85% of sequence in fragments shorter than   64119 bp
  90% of sequence in fragments shorter than   77518 bp
  95% of sequence in fragments shorter than  105923 bp

# Hpy99I.SalI (CGWCG+GTCGAC) double digest, 188 thousand fragments (157917+31396 sites), 15771 bp average fragment length
   5% of sequence in fragments shorter than    5932 bp
  10% of sequence in fragments shorter than    9797 bp
  15% of sequence in fragments shorter than   13376 bp
  20% of sequence in fragments shorter than   16887 bp
  25% of sequence in fragments shorter than   20452 bp
  30% of sequence in fragments shorter than   24212 bp
  35% of sequence in fragments shorter than   28130 bp
  40% of sequence in fragments shorter than   32166 bp
  45% of sequence in fragments shorter than   36603 bp
  50% of sequence in fragments shorter than   41523 bp
  55% of sequence in fragments shorter than   46770 bp
  60% of sequence in fragments shorter than   52550 bp
  65% of sequence in fragments shorter than   59192 bp
  70% of sequence in fragments shorter than   67231 bp
  75% of sequence in fragments shorter than   76022 bp
  80% of sequence in fragments shorter than   87732 bp
  85% of sequence in fragments shorter than  102485 bp
  90% of sequence in fragments shorter than  124538 bp
  95% of sequence in fragments shorter than  166841 bp

# SacII.XhoI (CCGCGG+CTCGAG) double digest, 187 thousand fragments (66901+121308 sites), 15858 bp average fragment length
   5% of sequence in fragments shorter than    5985 bp
  10% of sequence in fragments shorter than    9611 bp
  15% of sequence in fragments shorter than   12890 bp
  20% of sequence in fragments shorter than   16143 bp
  25% of sequence in fragments shorter than   19397 bp
  30% of sequence in fragments shorter than   22811 bp
  35% of sequence in fragments shorter than   26397 bp
  40% of sequence in fragments shorter than   30156 bp
  45% of sequence in fragments shorter than   34209 bp
  50% of sequence in fragments shorter than   38546 bp
  55% of sequence in fragments shorter than   43450 bp
  60% of sequence in fragments shorter than   48885 bp
  65% of sequence in fragments shorter than   54928 bp
  70% of sequence in fragments shorter than   62107 bp
  75% of sequence in fragments shorter than   70487 bp
  80% of sequence in fragments shorter than   80840 bp
  85% of sequence in fragments shorter than   95074 bp
  90% of sequence in fragments shorter than  117306 bp
  95% of sequence in fragments shorter than  163197 bp

# AclI.SgrAI (AACGTT+CRCCGGYG) double digest, 187 thousand fragments (172706+15270 sites), 15886 bp average fragment length
   5% of sequence in fragments shorter than    5528 bp
  10% of sequence in fragments shorter than    8636 bp
  15% of sequence in fragments shorter than   11227 bp
  20% of sequence in fragments shorter than   13649 bp
  25% of sequence in fragments shorter than   16023 bp
  30% of sequence in fragments shorter than   18394 bp
  35% of sequence in fragments shorter than   20823 bp
  40% of sequence in fragments shorter than   23283 bp
  45% of sequence in fragments shorter than   25816 bp
  50% of sequence in fragments shorter than   28556 bp
  55% of sequence in fragments shorter than   31516 bp
  60% of sequence in fragments shorter than   34663 bp
  65% of sequence in fragments shorter than   38297 bp
  70% of sequence in fragments shorter than   42337 bp
  75% of sequence in fragments shorter than   46967 bp
  80% of sequence in fragments shorter than   52541 bp
  85% of sequence in fragments shorter than   59952 bp
  90% of sequence in fragments shorter than   70041 bp
  95% of sequence in fragments shorter than   88679 bp

# AclI.NruI (AACGTT+TCGCGA) double digest, 187 thousand fragments (172706+14872 sites), 15919 bp average fragment length
   5% of sequence in fragments shorter than    5518 bp
  10% of sequence in fragments shorter than    8619 bp
  15% of sequence in fragments shorter than   11207 bp
  20% of sequence in fragments shorter than   13608 bp
  25% of sequence in fragments shorter than   15981 bp
  30% of sequence in fragments shorter than   18328 bp
  35% of sequence in fragments shorter than   20758 bp
  40% of sequence in fragments shorter than   23189 bp
  45% of sequence in fragments shorter than   25712 bp
  50% of sequence in fragments shorter than   28464 bp
  55% of sequence in fragments shorter than   31405 bp
  60% of sequence in fragments shorter than   34569 bp
  65% of sequence in fragments shorter than   38236 bp
  70% of sequence in fragments shorter than   42288 bp
  75% of sequence in fragments shorter than   46932 bp
  80% of sequence in fragments shorter than   52607 bp
  85% of sequence in fragments shorter than   59966 bp
  90% of sequence in fragments shorter than   70214 bp
  95% of sequence in fragments shorter than   89077 bp

# AclI.PvuI (AACGTT+CGATCG) double digest, 186 thousand fragments (172706+14186 sites), 15978 bp average fragment length
   5% of sequence in fragments shorter than    5523 bp
  10% of sequence in fragments shorter than    8648 bp
  15% of sequence in fragments shorter than   11265 bp
  20% of sequence in fragments shorter than   13702 bp
  25% of sequence in fragments shorter than   16123 bp
  30% of sequence in fragments shorter than   18486 bp
  35% of sequence in fragments shorter than   20918 bp
  40% of sequence in fragments shorter than   23392 bp
  45% of sequence in fragments shorter than   25958 bp
  50% of sequence in fragments shorter than   28676 bp
  55% of sequence in fragments shorter than   31677 bp
  60% of sequence in fragments shorter than   34939 bp
  65% of sequence in fragments shorter than   38614 bp
  70% of sequence in fragments shorter than   42717 bp
  75% of sequence in fragments shorter than   47474 bp
  80% of sequence in fragments shorter than   53228 bp
  85% of sequence in fragments shorter than   60753 bp
  90% of sequence in fragments shorter than   71266 bp
  95% of sequence in fragments shorter than   90055 bp

# AclI.FseI (AACGTT+GGCCGGCC) double digest, 185 thousand fragments (172706+13662 sites), 16023 bp average fragment length
   5% of sequence in fragments shorter than    5563 bp
  10% of sequence in fragments shorter than    8686 bp
  15% of sequence in fragments shorter than   11280 bp
  20% of sequence in fragments shorter than   13716 bp
  25% of sequence in fragments shorter than   16118 bp
  30% of sequence in fragments shorter than   18470 bp
  35% of sequence in fragments shorter than   20917 bp
  40% of sequence in fragments shorter than   23356 bp
  45% of sequence in fragments shorter than   25911 bp
  50% of sequence in fragments shorter than   28643 bp
  55% of sequence in fragments shorter than   31634 bp
  60% of sequence in fragments shorter than   34870 bp
  65% of sequence in fragments shorter than   38519 bp
  70% of sequence in fragments shorter than   42620 bp
  75% of sequence in fragments shorter than   47262 bp
  80% of sequence in fragments shorter than   52972 bp
  85% of sequence in fragments shorter than   60501 bp
  90% of sequence in fragments shorter than   70693 bp
  95% of sequence in fragments shorter than   89729 bp

# AgeI.SnaBI (ACCGGT+TACGTA) double digest, 185 thousand fragments (53386+132299 sites), 16077 bp average fragment length
   5% of sequence in fragments shorter than    5724 bp
  10% of sequence in fragments shorter than    8730 bp
  15% of sequence in fragments shorter than   11347 bp
  20% of sequence in fragments shorter than   13744 bp
  25% of sequence in fragments shorter than   16103 bp
  30% of sequence in fragments shorter than   18437 bp
  35% of sequence in fragments shorter than   20861 bp
  40% of sequence in fragments shorter than   23380 bp
  45% of sequence in fragments shorter than   26041 bp
  50% of sequence in fragments shorter than   28826 bp
  55% of sequence in fragments shorter than   31699 bp
  60% of sequence in fragments shorter than   34903 bp
  65% of sequence in fragments shorter than   38472 bp
  70% of sequence in fragments shorter than   42522 bp
  75% of sequence in fragments shorter than   47294 bp
  80% of sequence in fragments shorter than   53004 bp
  85% of sequence in fragments shorter than   60476 bp
  90% of sequence in fragments shorter than   71954 bp
  95% of sequence in fragments shorter than   94069 bp

# NaeI.PspXI (GCCGGC+VCTCGAGB) double digest, 184 thousand fragments (125692+59193 sites), 16141 bp average fragment length
   5% of sequence in fragments shorter than    5527 bp
  10% of sequence in fragments shorter than    9386 bp
  15% of sequence in fragments shorter than   13081 bp
  20% of sequence in fragments shorter than   16899 bp
  25% of sequence in fragments shorter than   20815 bp
  30% of sequence in fragments shorter than   24970 bp
  35% of sequence in fragments shorter than   29402 bp
  40% of sequence in fragments shorter than   34170 bp
  45% of sequence in fragments shorter than   39335 bp
  50% of sequence in fragments shorter than   45108 bp
  55% of sequence in fragments shorter than   51422 bp
  60% of sequence in fragments shorter than   58610 bp
  65% of sequence in fragments shorter than   66775 bp
  70% of sequence in fragments shorter than   76494 bp
  75% of sequence in fragments shorter than   88354 bp
  80% of sequence in fragments shorter than  102554 bp
  85% of sequence in fragments shorter than  122884 bp
  90% of sequence in fragments shorter than  155017 bp
  95% of sequence in fragments shorter than  221055 bp

# AclI.NotI (AACGTT+GCGGCCGC) double digest, 182 thousand fragments (172706+9795 sites), 16363 bp average fragment length
   5% of sequence in fragments shorter than    5716 bp
  10% of sequence in fragments shorter than    8913 bp
  15% of sequence in fragments shorter than   11600 bp
  20% of sequence in fragments shorter than   14121 bp
  25% of sequence in fragments shorter than   16585 bp
  30% of sequence in fragments shorter than   19044 bp
  35% of sequence in fragments shorter than   21534 bp
  40% of sequence in fragments shorter than   24068 bp
  45% of sequence in fragments shorter than   26690 bp
  50% of sequence in fragments shorter than   29466 bp
  55% of sequence in fragments shorter than   32562 bp
  60% of sequence in fragments shorter than   35905 bp
  65% of sequence in fragments shorter than   39670 bp
  70% of sequence in fragments shorter than   43800 bp
  75% of sequence in fragments shorter than   48662 bp
  80% of sequence in fragments shorter than   54464 bp
  85% of sequence in fragments shorter than   62150 bp
  90% of sequence in fragments shorter than   72661 bp
  95% of sequence in fragments shorter than   91745 bp

# AclI.RsrII (AACGTT+CGGWCCG) double digest, 182 thousand fragments (172706+9764 sites), 16366 bp average fragment length
   5% of sequence in fragments shorter than    5681 bp
  10% of sequence in fragments shorter than    8866 bp
  15% of sequence in fragments shorter than   11545 bp
  20% of sequence in fragments shorter than   14057 bp
  25% of sequence in fragments shorter than   16488 bp
  30% of sequence in fragments shorter than   18917 bp
  35% of sequence in fragments shorter than   21411 bp
  40% of sequence in fragments shorter than   23961 bp
  45% of sequence in fragments shorter than   26589 bp
  50% of sequence in fragments shorter than   29365 bp
  55% of sequence in fragments shorter than   32406 bp
  60% of sequence in fragments shorter than   35743 bp
  65% of sequence in fragments shorter than   39463 bp
  70% of sequence in fragments shorter than   43529 bp
  75% of sequence in fragments shorter than   48421 bp
  80% of sequence in fragments shorter than   54132 bp
  85% of sequence in fragments shorter than   61762 bp
  90% of sequence in fragments shorter than   72068 bp
  95% of sequence in fragments shorter than   91127 bp

# MluI.PacI (ACGCGT+TTAATTAA) double digest, 180 thousand fragments (21415+159455 sites), 16500 bp average fragment length
   5% of sequence in fragments shorter than    6104 bp
  10% of sequence in fragments shorter than    9261 bp
  15% of sequence in fragments shorter than   12002 bp
  20% of sequence in fragments shorter than   14574 bp
  25% of sequence in fragments shorter than   17136 bp
  30% of sequence in fragments shorter than   19687 bp
  35% of sequence in fragments shorter than   22348 bp
  40% of sequence in fragments shorter than   25035 bp
  45% of sequence in fragments shorter than   27889 bp
  50% of sequence in fragments shorter than   30891 bp
  55% of sequence in fragments shorter than   34194 bp
  60% of sequence in fragments shorter than   37823 bp
  65% of sequence in fragments shorter than   41912 bp
  70% of sequence in fragments shorter than   46566 bp
  75% of sequence in fragments shorter than   52130 bp
  80% of sequence in fragments shorter than   59084 bp
  85% of sequence in fragments shorter than   68571 bp
  90% of sequence in fragments shorter than   82271 bp
  95% of sequence in fragments shorter than  112002 bp

# PspXI.XhoI (VCTCGAGB+CTCGAG) double digest, 180 thousand fragments (59193+121308 sites), 16536 bp average fragment length
   5% of sequence in fragments shorter than    8388 bp
  10% of sequence in fragments shorter than   12847 bp
  15% of sequence in fragments shorter than   16882 bp
  20% of sequence in fragments shorter than   20792 bp
  25% of sequence in fragments shorter than   24704 bp
  30% of sequence in fragments shorter than   28584 bp
  35% of sequence in fragments shorter than   32862 bp
  40% of sequence in fragments shorter than   37291 bp
  45% of sequence in fragments shorter than   42004 bp
  50% of sequence in fragments shorter than   47068 bp
  55% of sequence in fragments shorter than   52589 bp
  60% of sequence in fragments shorter than   58831 bp
  65% of sequence in fragments shorter than   65742 bp
  70% of sequence in fragments shorter than   73860 bp
  75% of sequence in fragments shorter than   83108 bp
  80% of sequence in fragments shorter than   94838 bp
  85% of sequence in fragments shorter than  111037 bp
  90% of sequence in fragments shorter than  134724 bp
  95% of sequence in fragments shorter than  185020 bp

# Hpy99I.MluI (CGWCG+ACGCGT) double digest, 178 thousand fragments (157917+21415 sites), 16650 bp average fragment length
   5% of sequence in fragments shorter than    6096 bp
  10% of sequence in fragments shorter than   10310 bp
  15% of sequence in fragments shorter than   14264 bp
  20% of sequence in fragments shorter than   18182 bp
  25% of sequence in fragments shorter than   22219 bp
  30% of sequence in fragments shorter than   26416 bp
  35% of sequence in fragments shorter than   30789 bp
  40% of sequence in fragments shorter than   35495 bp
  45% of sequence in fragments shorter than   40687 bp
  50% of sequence in fragments shorter than   46265 bp
  55% of sequence in fragments shorter than   52206 bp
  60% of sequence in fragments shorter than   59127 bp
  65% of sequence in fragments shorter than   67287 bp
  70% of sequence in fragments shorter than   76136 bp
  75% of sequence in fragments shorter than   87463 bp
  80% of sequence in fragments shorter than  101765 bp
  85% of sequence in fragments shorter than  120051 bp
  90% of sequence in fragments shorter than  146732 bp
  95% of sequence in fragments shorter than  198525 bp

# AgeI.NaeI (ACCGGT+GCCGGC) double digest, 178 thousand fragments (53386+125692 sites), 16665 bp average fragment length
   5% of sequence in fragments shorter than    5886 bp
  10% of sequence in fragments shorter than    9812 bp
  15% of sequence in fragments shorter than   13478 bp
  20% of sequence in fragments shorter than   17133 bp
  25% of sequence in fragments shorter than   20832 bp
  30% of sequence in fragments shorter than   24731 bp
  35% of sequence in fragments shorter than   28920 bp
  40% of sequence in fragments shorter than   33295 bp
  45% of sequence in fragments shorter than   37907 bp
  50% of sequence in fragments shorter than   42897 bp
  55% of sequence in fragments shorter than   48401 bp
  60% of sequence in fragments shorter than   54495 bp
  65% of sequence in fragments shorter than   61839 bp
  70% of sequence in fragments shorter than   70053 bp
  75% of sequence in fragments shorter than   79835 bp
  80% of sequence in fragments shorter than   92235 bp
  85% of sequence in fragments shorter than  108447 bp
  90% of sequence in fragments shorter than  134154 bp
  95% of sequence in fragments shorter than  186888 bp

# AclI.AscI (AACGTT+GGCGCGCC) double digest, 177 thousand fragments (172706+4751 sites), 16829 bp average fragment length
   5% of sequence in fragments shorter than    5849 bp
  10% of sequence in fragments shorter than    9110 bp
  15% of sequence in fragments shorter than   11845 bp
  20% of sequence in fragments shorter than   14416 bp
  25% of sequence in fragments shorter than   16918 bp
  30% of sequence in fragments shorter than   19438 bp
  35% of sequence in fragments shorter than   21993 bp
  40% of sequence in fragments shorter than   24585 bp
  45% of sequence in fragments shorter than   27310 bp
  50% of sequence in fragments shorter than   30189 bp
  55% of sequence in fragments shorter than   33273 bp
  60% of sequence in fragments shorter than   36739 bp
  65% of sequence in fragments shorter than   40571 bp
  70% of sequence in fragments shorter than   44869 bp
  75% of sequence in fragments shorter than   49790 bp
  80% of sequence in fragments shorter than   55862 bp
  85% of sequence in fragments shorter than   63762 bp
  90% of sequence in fragments shorter than   74664 bp
  95% of sequence in fragments shorter than   95330 bp

# PacI.SgrAI (TTAATTAA+CRCCGGYG) double digest, 174 thousand fragments (159455+15270 sites), 17078 bp average fragment length
   5% of sequence in fragments shorter than    6322 bp
  10% of sequence in fragments shorter than    9599 bp
  15% of sequence in fragments shorter than   12450 bp
  20% of sequence in fragments shorter than   15162 bp
  25% of sequence in fragments shorter than   17799 bp
  30% of sequence in fragments shorter than   20458 bp
  35% of sequence in fragments shorter than   23224 bp
  40% of sequence in fragments shorter than   26096 bp
  45% of sequence in fragments shorter than   29014 bp
  50% of sequence in fragments shorter than   32184 bp
  55% of sequence in fragments shorter than   35687 bp
  60% of sequence in fragments shorter than   39473 bp
  65% of sequence in fragments shorter than   43671 bp
  70% of sequence in fragments shorter than   48683 bp
  75% of sequence in fragments shorter than   54490 bp
  80% of sequence in fragments shorter than   62111 bp
  85% of sequence in fragments shorter than   71818 bp
  90% of sequence in fragments shorter than   87127 bp
  95% of sequence in fragments shorter than  118021 bp

# AgeI.XhoI (ACCGGT+CTCGAG) double digest, 174 thousand fragments (53386+121308 sites), 17089 bp average fragment length
   5% of sequence in fragments shorter than    5894 bp
  10% of sequence in fragments shorter than    9097 bp
  15% of sequence in fragments shorter than   11926 bp
  20% of sequence in fragments shorter than   14670 bp
  25% of sequence in fragments shorter than   17328 bp
  30% of sequence in fragments shorter than   20065 bp
  35% of sequence in fragments shorter than   22951 bp
  40% of sequence in fragments shorter than   25994 bp
  45% of sequence in fragments shorter than   29162 bp
  50% of sequence in fragments shorter than   32669 bp
  55% of sequence in fragments shorter than   36496 bp
  60% of sequence in fragments shorter than   40611 bp
  65% of sequence in fragments shorter than   45351 bp
  70% of sequence in fragments shorter than   50853 bp
  75% of sequence in fragments shorter than   57314 bp
  80% of sequence in fragments shorter than   65240 bp
  85% of sequence in fragments shorter than   76029 bp
  90% of sequence in fragments shorter than   91968 bp
  95% of sequence in fragments shorter than  126018 bp

# NruI.PacI (TCGCGA+TTAATTAA) double digest, 174 thousand fragments (14872+159455 sites), 17119 bp average fragment length
   5% of sequence in fragments shorter than    6308 bp
  10% of sequence in fragments shorter than    9583 bp
  15% of sequence in fragments shorter than   12426 bp
  20% of sequence in fragments shorter than   15121 bp
  25% of sequence in fragments shorter than   17759 bp
  30% of sequence in fragments shorter than   20430 bp
  35% of sequence in fragments shorter than   23194 bp
  40% of sequence in fragments shorter than   26055 bp
  45% of sequence in fragments shorter than   29032 bp
  50% of sequence in fragments shorter than   32230 bp
  55% of sequence in fragments shorter than   35705 bp
  60% of sequence in fragments shorter than   39521 bp
  65% of sequence in fragments shorter than   43771 bp
  70% of sequence in fragments shorter than   48908 bp
  75% of sequence in fragments shorter than   54891 bp
  80% of sequence in fragments shorter than   62567 bp
  85% of sequence in fragments shorter than   72655 bp
  90% of sequence in fragments shorter than   88394 bp
  95% of sequence in fragments shorter than  122316 bp

# PmeI.SnaBI (GTTTAAAC+TACGTA) double digest, 174 thousand fragments (42094+132299 sites), 17122 bp average fragment length
   5% of sequence in fragments shorter than    6016 bp
  10% of sequence in fragments shorter than    9287 bp
  15% of sequence in fragments shorter than   12074 bp
  20% of sequence in fragments shorter than   14716 bp
  25% of sequence in fragments shorter than   17335 bp
  30% of sequence in fragments shorter than   19918 bp
  35% of sequence in fragments shorter than   22612 bp
  40% of sequence in fragments shorter than   25368 bp
  45% of sequence in fragments shorter than   28229 bp
  50% of sequence in fragments shorter than   31269 bp
  55% of sequence in fragments shorter than   34675 bp
  60% of sequence in fragments shorter than   38452 bp
  65% of sequence in fragments shorter than   42511 bp
  70% of sequence in fragments shorter than   47296 bp
  75% of sequence in fragments shorter than   52981 bp
  80% of sequence in fragments shorter than   60059 bp
  85% of sequence in fragments shorter than   69393 bp
  90% of sequence in fragments shorter than   83667 bp
  95% of sequence in fragments shorter than  112023 bp

# PacI.PvuI (TTAATTAA+CGATCG) double digest, 173 thousand fragments (159455+14186 sites), 17187 bp average fragment length
   5% of sequence in fragments shorter than    6315 bp
  10% of sequence in fragments shorter than    9631 bp
  15% of sequence in fragments shorter than   12525 bp
  20% of sequence in fragments shorter than   15261 bp
  25% of sequence in fragments shorter than   17937 bp
  30% of sequence in fragments shorter than   20672 bp
  35% of sequence in fragments shorter than   23493 bp
  40% of sequence in fragments shorter than   26392 bp
  45% of sequence in fragments shorter than   29380 bp
  50% of sequence in fragments shorter than   32608 bp
  55% of sequence in fragments shorter than   36207 bp
  60% of sequence in fragments shorter than   40186 bp
  65% of sequence in fragments shorter than   44570 bp
  70% of sequence in fragments shorter than   49782 bp
  75% of sequence in fragments shorter than   56012 bp
  80% of sequence in fragments shorter than   63680 bp
  85% of sequence in fragments shorter than   73931 bp
  90% of sequence in fragments shorter than   89590 bp
  95% of sequence in fragments shorter than  120569 bp

# FseI.PacI (GGCCGGCC+TTAATTAA) double digest, 172 thousand fragments (13662+159455 sites), 17237 bp average fragment length
   5% of sequence in fragments shorter than    6390 bp
  10% of sequence in fragments shorter than    9682 bp
  15% of sequence in fragments shorter than   12553 bp
  20% of sequence in fragments shorter than   15282 bp
  25% of sequence in fragments shorter than   17916 bp
  30% of sequence in fragments shorter than   20623 bp
  35% of sequence in fragments shorter than   23386 bp
  40% of sequence in fragments shorter than   26269 bp
  45% of sequence in fragments shorter than   29201 bp
  50% of sequence in fragments shorter than   32392 bp
  55% of sequence in fragments shorter than   35892 bp
  60% of sequence in fragments shorter than   39703 bp
  65% of sequence in fragments shorter than   43903 bp
  70% of sequence in fragments shorter than   48869 bp
  75% of sequence in fragments shorter than   54786 bp
  80% of sequence in fragments shorter than   62198 bp
  85% of sequence in fragments shorter than   71948 bp
  90% of sequence in fragments shorter than   86886 bp
  95% of sequence in fragments shorter than  118994 bp

# Hpy99I.SgrAI (CGWCG+CRCCGGYG) double digest, 172 thousand fragments (157917+15270 sites), 17241 bp average fragment length
   5% of sequence in fragments shorter than    6478 bp
  10% of sequence in fragments shorter than   10935 bp
  15% of sequence in fragments shorter than   15094 bp
  20% of sequence in fragments shorter than   19298 bp
  25% of sequence in fragments shorter than   23562 bp
  30% of sequence in fragments shorter than   28017 bp
  35% of sequence in fragments shorter than   32754 bp
  40% of sequence in fragments shorter than   37887 bp
  45% of sequence in fragments shorter than   43416 bp
  50% of sequence in fragments shorter than   49294 bp
  55% of sequence in fragments shorter than   55727 bp
  60% of sequence in fragments shorter than   63163 bp
  65% of sequence in fragments shorter than   71339 bp
  70% of sequence in fragments shorter than   81392 bp
  75% of sequence in fragments shorter than   93202 bp
  80% of sequence in fragments shorter than  108237 bp
  85% of sequence in fragments shorter than  127487 bp
  90% of sequence in fragments shorter than  155955 bp
  95% of sequence in fragments shorter than  215579 bp

# Hpy99I.NruI (CGWCG+TCGCGA) double digest, 172 thousand fragments (157917+14872 sites), 17281 bp average fragment length
   5% of sequence in fragments shorter than    6407 bp
  10% of sequence in fragments shorter than   10743 bp
  15% of sequence in fragments shorter than   14779 bp
  20% of sequence in fragments shorter than   18831 bp
  25% of sequence in fragments shorter than   23012 bp
  30% of sequence in fragments shorter than   27320 bp
  35% of sequence in fragments shorter than   31878 bp
  40% of sequence in fragments shorter than   36755 bp
  45% of sequence in fragments shorter than   42090 bp
  50% of sequence in fragments shorter than   47803 bp
  55% of sequence in fragments shorter than   53944 bp
  60% of sequence in fragments shorter than   61162 bp
  65% of sequence in fragments shorter than   69312 bp
  70% of sequence in fragments shorter than   78682 bp
  75% of sequence in fragments shorter than   90179 bp
  80% of sequence in fragments shorter than  104565 bp
  85% of sequence in fragments shorter than  124035 bp
  90% of sequence in fragments shorter than  150762 bp
  95% of sequence in fragments shorter than  206774 bp

# Hpy99I.PvuI (CGWCG+CGATCG) double digest, 171 thousand fragments (157917+14186 sites), 17351 bp average fragment length
   5% of sequence in fragments shorter than    6341 bp
  10% of sequence in fragments shorter than   10709 bp
  15% of sequence in fragments shorter than   14805 bp
  20% of sequence in fragments shorter than   18850 bp
  25% of sequence in fragments shorter than   23017 bp
  30% of sequence in fragments shorter than   27392 bp
  35% of sequence in fragments shorter than   31918 bp
  40% of sequence in fragments shorter than   36841 bp
  45% of sequence in fragments shorter than   42092 bp
  50% of sequence in fragments shorter than   47929 bp
  55% of sequence in fragments shorter than   54243 bp
  60% of sequence in fragments shorter than   61395 bp
  65% of sequence in fragments shorter than   69508 bp
  70% of sequence in fragments shorter than   79052 bp
  75% of sequence in fragments shorter than   90343 bp
  80% of sequence in fragments shorter than  105182 bp
  85% of sequence in fragments shorter than  124171 bp
  90% of sequence in fragments shorter than  152218 bp
  95% of sequence in fragments shorter than  207537 bp

# FseI.Hpy99I (GGCCGGCC+CGWCG) double digest, 171 thousand fragments (13662+157917 sites), 17403 bp average fragment length
   5% of sequence in fragments shorter than    6410 bp
  10% of sequence in fragments shorter than   10902 bp
  15% of sequence in fragments shorter than   15105 bp
  20% of sequence in fragments shorter than   19342 bp
  25% of sequence in fragments shorter than   23746 bp
  30% of sequence in fragments shorter than   28334 bp
  35% of sequence in fragments shorter than   33127 bp
  40% of sequence in fragments shorter than   38393 bp
  45% of sequence in fragments shorter than   43941 bp
  50% of sequence in fragments shorter than   50085 bp
  55% of sequence in fragments shorter than   56745 bp
  60% of sequence in fragments shorter than   64432 bp
  65% of sequence in fragments shorter than   72937 bp
  70% of sequence in fragments shorter than   83378 bp
  75% of sequence in fragments shorter than   95448 bp
  80% of sequence in fragments shorter than  110902 bp
  85% of sequence in fragments shorter than  130164 bp
  90% of sequence in fragments shorter than  160283 bp
  95% of sequence in fragments shorter than  218742 bp

# NotI.PacI (GCGGCCGC+TTAATTAA) double digest, 169 thousand fragments (9795+159455 sites), 17631 bp average fragment length
   5% of sequence in fragments shorter than    6530 bp
  10% of sequence in fragments shorter than    9928 bp
  15% of sequence in fragments shorter than   12903 bp
  20% of sequence in fragments shorter than   15696 bp
  25% of sequence in fragments shorter than   18434 bp
  30% of sequence in fragments shorter than   21243 bp
  35% of sequence in fragments shorter than   24113 bp
  40% of sequence in fragments shorter than   27122 bp
  45% of sequence in fragments shorter than   30237 bp
  50% of sequence in fragments shorter than   33577 bp
  55% of sequence in fragments shorter than   37257 bp
  60% of sequence in fragments shorter than   41273 bp
  65% of sequence in fragments shorter than   45726 bp
  70% of sequence in fragments shorter than   51047 bp
  75% of sequence in fragments shorter than   57387 bp
  80% of sequence in fragments shorter than   65641 bp
  85% of sequence in fragments shorter than   76471 bp
  90% of sequence in fragments shorter than   92431 bp
  95% of sequence in fragments shorter than  128363 bp

# PacI.RsrII (TTAATTAA+CGGWCCG) double digest, 168 thousand fragments (159455+9764 sites), 17636 bp average fragment length
   5% of sequence in fragments shorter than    6502 bp
  10% of sequence in fragments shorter than    9874 bp
  15% of sequence in fragments shorter than   12831 bp
  20% of sequence in fragments shorter than   15629 bp
  25% of sequence in fragments shorter than   18354 bp
  30% of sequence in fragments shorter than   21141 bp
  35% of sequence in fragments shorter than   24021 bp
  40% of sequence in fragments shorter than   26983 bp
  45% of sequence in fragments shorter than   30092 bp
  50% of sequence in fragments shorter than   33425 bp
  55% of sequence in fragments shorter than   37115 bp
  60% of sequence in fragments shorter than   41079 bp
  65% of sequence in fragments shorter than   45466 bp
  70% of sequence in fragments shorter than   50734 bp
  75% of sequence in fragments shorter than   56948 bp
  80% of sequence in fragments shorter than   64890 bp
  85% of sequence in fragments shorter than   75489 bp
  90% of sequence in fragments shorter than   91461 bp
  95% of sequence in fragments shorter than  125326 bp

# NaeI.PmeI (GCCGGC+GTTTAAAC) double digest, 167 thousand fragments (125692+42094 sites), 17791 bp average fragment length
   5% of sequence in fragments shorter than    6447 bp
  10% of sequence in fragments shorter than   10545 bp
  15% of sequence in fragments shorter than   14312 bp
  20% of sequence in fragments shorter than   18070 bp
  25% of sequence in fragments shorter than   21790 bp
  30% of sequence in fragments shorter than   25507 bp
  35% of sequence in fragments shorter than   29483 bp
  40% of sequence in fragments shorter than   33598 bp
  45% of sequence in fragments shorter than   38151 bp
  50% of sequence in fragments shorter than   42940 bp
  55% of sequence in fragments shorter than   48281 bp
  60% of sequence in fragments shorter than   54037 bp
  65% of sequence in fragments shorter than   60512 bp
  70% of sequence in fragments shorter than   68144 bp
  75% of sequence in fragments shorter than   77131 bp
  80% of sequence in fragments shorter than   88145 bp
  85% of sequence in fragments shorter than  102343 bp
  90% of sequence in fragments shorter than  123136 bp
  95% of sequence in fragments shorter than  168322 bp

# Hpy99I.NotI (CGWCG+GCGGCCGC) double digest, 167 thousand fragments (157917+9795 sites), 17805 bp average fragment length
   5% of sequence in fragments shorter than    6744 bp
  10% of sequence in fragments shorter than   11383 bp
  15% of sequence in fragments shorter than   15805 bp
  20% of sequence in fragments shorter than   20204 bp
  25% of sequence in fragments shorter than   24804 bp
  30% of sequence in fragments shorter than   29536 bp
  35% of sequence in fragments shorter than   34450 bp
  40% of sequence in fragments shorter than   39792 bp
  45% of sequence in fragments shorter than   45488 bp
  50% of sequence in fragments shorter than   51701 bp
  55% of sequence in fragments shorter than   58483 bp
  60% of sequence in fragments shorter than   66514 bp
  65% of sequence in fragments shorter than   75121 bp
  70% of sequence in fragments shorter than   85800 bp
  75% of sequence in fragments shorter than   98429 bp
  80% of sequence in fragments shorter than  114471 bp
  85% of sequence in fragments shorter than  134994 bp
  90% of sequence in fragments shorter than  165745 bp
  95% of sequence in fragments shorter than  228010 bp

# Hpy99I.RsrII (CGWCG+CGGWCCG) double digest, 167 thousand fragments (157917+9764 sites), 17808 bp average fragment length
   5% of sequence in fragments shorter than    6632 bp
  10% of sequence in fragments shorter than   11195 bp
  15% of sequence in fragments shorter than   15553 bp
  20% of sequence in fragments shorter than   19870 bp
  25% of sequence in fragments shorter than   24331 bp
  30% of sequence in fragments shorter than   28981 bp
  35% of sequence in fragments shorter than   33781 bp
  40% of sequence in fragments shorter than   39085 bp
  45% of sequence in fragments shorter than   44658 bp
  50% of sequence in fragments shorter than   50815 bp
  55% of sequence in fragments shorter than   57441 bp
  60% of sequence in fragments shorter than   65301 bp
  65% of sequence in fragments shorter than   73756 bp
  70% of sequence in fragments shorter than   84017 bp
  75% of sequence in fragments shorter than   96617 bp
  80% of sequence in fragments shorter than  112188 bp
  85% of sequence in fragments shorter than  131398 bp
  90% of sequence in fragments shorter than  161663 bp
  95% of sequence in fragments shorter than  222635 bp

# AscI.PacI (GGCGCGCC+TTAATTAA) double digest, 163 thousand fragments (4751+159455 sites), 18174 bp average fragment length
   5% of sequence in fragments shorter than    6685 bp
  10% of sequence in fragments shorter than   10147 bp
  15% of sequence in fragments shorter than   13191 bp
  20% of sequence in fragments shorter than   16084 bp
  25% of sequence in fragments shorter than   18944 bp
  30% of sequence in fragments shorter than   21887 bp
  35% of sequence in fragments shorter than   24828 bp
  40% of sequence in fragments shorter than   27916 bp
  45% of sequence in fragments shorter than   31179 bp
  50% of sequence in fragments shorter than   34684 bp
  55% of sequence in fragments shorter than   38640 bp
  60% of sequence in fragments shorter than   42777 bp
  65% of sequence in fragments shorter than   47683 bp
  70% of sequence in fragments shorter than   53267 bp
  75% of sequence in fragments shorter than   60205 bp
  80% of sequence in fragments shorter than   68829 bp
  85% of sequence in fragments shorter than   80462 bp
  90% of sequence in fragments shorter than   98389 bp
  95% of sequence in fragments shorter than  138885 bp

# SalI.SnaBI (GTCGAC+TACGTA) double digest, 163 thousand fragments (31396+132299 sites), 18242 bp average fragment length
   5% of sequence in fragments shorter than    6506 bp
  10% of sequence in fragments shorter than    9965 bp
  15% of sequence in fragments shorter than   12949 bp
  20% of sequence in fragments shorter than   15779 bp
  25% of sequence in fragments shorter than   18492 bp
  30% of sequence in fragments shorter than   21275 bp
  35% of sequence in fragments shorter than   24048 bp
  40% of sequence in fragments shorter than   26933 bp
  45% of sequence in fragments shorter than   29968 bp
  50% of sequence in fragments shorter than   33174 bp
  55% of sequence in fragments shorter than   36555 bp
  60% of sequence in fragments shorter than   40332 bp
  65% of sequence in fragments shorter than   44559 bp
  70% of sequence in fragments shorter than   49208 bp
  75% of sequence in fragments shorter than   54892 bp
  80% of sequence in fragments shorter than   61730 bp
  85% of sequence in fragments shorter than   70626 bp
  90% of sequence in fragments shorter than   83878 bp
  95% of sequence in fragments shorter than  110230 bp

# PmeI.XhoI (GTTTAAAC+CTCGAG) double digest, 163 thousand fragments (42094+121308 sites), 18274 bp average fragment length
   5% of sequence in fragments shorter than    6422 bp
  10% of sequence in fragments shorter than    9708 bp
  15% of sequence in fragments shorter than   12574 bp
  20% of sequence in fragments shorter than   15295 bp
  25% of sequence in fragments shorter than   17944 bp
  30% of sequence in fragments shorter than   20664 bp
  35% of sequence in fragments shorter than   23447 bp
  40% of sequence in fragments shorter than   26355 bp
  45% of sequence in fragments shorter than   29402 bp
  50% of sequence in fragments shorter than   32711 bp
  55% of sequence in fragments shorter than   36228 bp
  60% of sequence in fragments shorter than   40152 bp
  65% of sequence in fragments shorter than   44580 bp
  70% of sequence in fragments shorter than   49579 bp
  75% of sequence in fragments shorter than   55332 bp
  80% of sequence in fragments shorter than   62366 bp
  85% of sequence in fragments shorter than   71742 bp
  90% of sequence in fragments shorter than   85585 bp
  95% of sequence in fragments shorter than  113512 bp

# AscI.Hpy99I (GGCGCGCC+CGWCG) double digest, 162 thousand fragments (4751+157917 sites), 18358 bp average fragment length
   5% of sequence in fragments shorter than    6849 bp
  10% of sequence in fragments shorter than   11535 bp
  15% of sequence in fragments shorter than   15966 bp
  20% of sequence in fragments shorter than   20378 bp
  25% of sequence in fragments shorter than   25004 bp
  30% of sequence in fragments shorter than   29756 bp
  35% of sequence in fragments shorter than   34743 bp
  40% of sequence in fragments shorter than   40049 bp
  45% of sequence in fragments shorter than   45789 bp
  50% of sequence in fragments shorter than   52043 bp
  55% of sequence in fragments shorter than   58968 bp
  60% of sequence in fragments shorter than   67011 bp
  65% of sequence in fragments shorter than   75606 bp
  70% of sequence in fragments shorter than   86238 bp
  75% of sequence in fragments shorter than   98922 bp
  80% of sequence in fragments shorter than  115016 bp
  85% of sequence in fragments shorter than  135225 bp
  90% of sequence in fragments shorter than  165873 bp
  95% of sequence in fragments shorter than  228276 bp

# AatII.ClaI (GACGTC+ATCGAT) double digest, 157 thousand fragments (71089+86477 sites), 18952 bp average fragment length
   5% of sequence in fragments shorter than    6638 bp
  10% of sequence in fragments shorter than   10094 bp
  15% of sequence in fragments shorter than   13116 bp
  20% of sequence in fragments shorter than   15968 bp
  25% of sequence in fragments shorter than   18733 bp
  30% of sequence in fragments shorter than   21559 bp
  35% of sequence in fragments shorter than   24433 bp
  40% of sequence in fragments shorter than   27360 bp
  45% of sequence in fragments shorter than   30463 bp
  50% of sequence in fragments shorter than   33860 bp
  55% of sequence in fragments shorter than   37387 bp
  60% of sequence in fragments shorter than   41397 bp
  65% of sequence in fragments shorter than   45815 bp
  70% of sequence in fragments shorter than   50664 bp
  75% of sequence in fragments shorter than   56441 bp
  80% of sequence in fragments shorter than   63260 bp
  85% of sequence in fragments shorter than   72151 bp
  90% of sequence in fragments shorter than   85204 bp
  95% of sequence in fragments shorter than  110606 bp

# NaeI.SalI (GCCGGC+GTCGAC) double digest, 156 thousand fragments (125692+31396 sites), 19003 bp average fragment length
   5% of sequence in fragments shorter than    6687 bp
  10% of sequence in fragments shorter than   11339 bp
  15% of sequence in fragments shorter than   15745 bp
  20% of sequence in fragments shorter than   20249 bp
  25% of sequence in fragments shorter than   24952 bp
  30% of sequence in fragments shorter than   29829 bp
  35% of sequence in fragments shorter than   35048 bp
  40% of sequence in fragments shorter than   40611 bp
  45% of sequence in fragments shorter than   46566 bp
  50% of sequence in fragments shorter than   52988 bp
  55% of sequence in fragments shorter than   60064 bp
  60% of sequence in fragments shorter than   68314 bp
  65% of sequence in fragments shorter than   77569 bp
  70% of sequence in fragments shorter than   88450 bp
  75% of sequence in fragments shorter than  101698 bp
  80% of sequence in fragments shorter than  117386 bp
  85% of sequence in fragments shorter than  138153 bp
  90% of sequence in fragments shorter than  171602 bp
  95% of sequence in fragments shorter than  247894 bp

# MluI.SnaBI (ACGCGT+TACGTA) double digest, 153 thousand fragments (21415+132299 sites), 19428 bp average fragment length
   5% of sequence in fragments shorter than    6949 bp
  10% of sequence in fragments shorter than   10730 bp
  15% of sequence in fragments shorter than   13921 bp
  20% of sequence in fragments shorter than   16923 bp
  25% of sequence in fragments shorter than   19827 bp
  30% of sequence in fragments shorter than   22862 bp
  35% of sequence in fragments shorter than   25903 bp
  40% of sequence in fragments shorter than   29040 bp
  45% of sequence in fragments shorter than   32208 bp
  50% of sequence in fragments shorter than   35686 bp
  55% of sequence in fragments shorter than   39466 bp
  60% of sequence in fragments shorter than   43533 bp
  65% of sequence in fragments shorter than   48075 bp
  70% of sequence in fragments shorter than   53264 bp
  75% of sequence in fragments shorter than   59212 bp
  80% of sequence in fragments shorter than   66886 bp
  85% of sequence in fragments shorter than   76810 bp
  90% of sequence in fragments shorter than   90999 bp
  95% of sequence in fragments shorter than  119349 bp

# ClaI.SacII (ATCGAT+CCGCGG) double digest, 153 thousand fragments (86477+66901 sites), 19466 bp average fragment length
   5% of sequence in fragments shorter than    7793 bp
  10% of sequence in fragments shorter than   12205 bp
  15% of sequence in fragments shorter than   16124 bp
  20% of sequence in fragments shorter than   19804 bp
  25% of sequence in fragments shorter than   23393 bp
  30% of sequence in fragments shorter than   27006 bp
  35% of sequence in fragments shorter than   30711 bp
  40% of sequence in fragments shorter than   34565 bp
  45% of sequence in fragments shorter than   38583 bp
  50% of sequence in fragments shorter than   42837 bp
  55% of sequence in fragments shorter than   47361 bp
  60% of sequence in fragments shorter than   52337 bp
  65% of sequence in fragments shorter than   57996 bp
  70% of sequence in fragments shorter than   64574 bp
  75% of sequence in fragments shorter than   72195 bp
  80% of sequence in fragments shorter than   81586 bp
  85% of sequence in fragments shorter than   94007 bp
  90% of sequence in fragments shorter than  112617 bp
  95% of sequence in fragments shorter than  147690 bp

# SalI.XhoI (GTCGAC+CTCGAG) double digest, 152 thousand fragments (31396+121308 sites), 19556 bp average fragment length
   5% of sequence in fragments shorter than    6742 bp
  10% of sequence in fragments shorter than   10308 bp
  15% of sequence in fragments shorter than   13489 bp
  20% of sequence in fragments shorter than   16593 bp
  25% of sequence in fragments shorter than   19667 bp
  30% of sequence in fragments shorter than   22832 bp
  35% of sequence in fragments shorter than   26054 bp
  40% of sequence in fragments shorter than   29401 bp
  45% of sequence in fragments shorter than   33097 bp
  50% of sequence in fragments shorter than   37041 bp
  55% of sequence in fragments shorter than   41361 bp
  60% of sequence in fragments shorter than   46040 bp
  65% of sequence in fragments shorter than   51313 bp
  70% of sequence in fragments shorter than   57597 bp
  75% of sequence in fragments shorter than   65180 bp
  80% of sequence in fragments shorter than   74269 bp
  85% of sequence in fragments shorter than   86087 bp
  90% of sequence in fragments shorter than  103488 bp
  95% of sequence in fragments shorter than  142793 bp

# SgrAI.SnaBI (CRCCGGYG+TACGTA) double digest, 147 thousand fragments (15270+132299 sites), 20236 bp average fragment length
   5% of sequence in fragments shorter than    7292 bp
  10% of sequence in fragments shorter than   11241 bp
  15% of sequence in fragments shorter than   14553 bp
  20% of sequence in fragments shorter than   17663 bp
  25% of sequence in fragments shorter than   20798 bp
  30% of sequence in fragments shorter than   23894 bp
  35% of sequence in fragments shorter than   27026 bp
  40% of sequence in fragments shorter than   30276 bp
  45% of sequence in fragments shorter than   33594 bp
  50% of sequence in fragments shorter than   37145 bp
  55% of sequence in fragments shorter than   41063 bp
  60% of sequence in fragments shorter than   45311 bp
  65% of sequence in fragments shorter than   49820 bp
  70% of sequence in fragments shorter than   55108 bp
  75% of sequence in fragments shorter than   61412 bp
  80% of sequence in fragments shorter than   69426 bp
  85% of sequence in fragments shorter than   79653 bp
  90% of sequence in fragments shorter than   95283 bp
  95% of sequence in fragments shorter than  126306 bp

# NruI.SnaBI (TCGCGA+TACGTA) double digest, 146 thousand fragments (14872+132299 sites), 20291 bp average fragment length
   5% of sequence in fragments shorter than    7253 bp
  10% of sequence in fragments shorter than   11178 bp
  15% of sequence in fragments shorter than   14488 bp
  20% of sequence in fragments shorter than   17613 bp
  25% of sequence in fragments shorter than   20763 bp
  30% of sequence in fragments shorter than   23872 bp
  35% of sequence in fragments shorter than   27013 bp
  40% of sequence in fragments shorter than   30237 bp
  45% of sequence in fragments shorter than   33600 bp
  50% of sequence in fragments shorter than   37163 bp
  55% of sequence in fragments shorter than   41076 bp
  60% of sequence in fragments shorter than   45424 bp
  65% of sequence in fragments shorter than   49987 bp
  70% of sequence in fragments shorter than   55438 bp
  75% of sequence in fragments shorter than   61803 bp
  80% of sequence in fragments shorter than   69665 bp
  85% of sequence in fragments shorter than   79867 bp
  90% of sequence in fragments shorter than   95561 bp
  95% of sequence in fragments shorter than  126711 bp

# MluI.NaeI (ACGCGT+GCCGGC) double digest, 146 thousand fragments (21415+125692 sites), 20292 bp average fragment length
   5% of sequence in fragments shorter than    7085 bp
  10% of sequence in fragments shorter than   12277 bp
  15% of sequence in fragments shorter than   17483 bp
  20% of sequence in fragments shorter than   22851 bp
  25% of sequence in fragments shorter than   28454 bp
  30% of sequence in fragments shorter than   34393 bp
  35% of sequence in fragments shorter than   40600 bp
  40% of sequence in fragments shorter than   47530 bp
  45% of sequence in fragments shorter than   54887 bp
  50% of sequence in fragments shorter than   63163 bp
  55% of sequence in fragments shorter than   72744 bp
  60% of sequence in fragments shorter than   83538 bp
  65% of sequence in fragments shorter than   96321 bp
  70% of sequence in fragments shorter than  111012 bp
  75% of sequence in fragments shorter than  129253 bp
  80% of sequence in fragments shorter than  152434 bp
  85% of sequence in fragments shorter than  182739 bp
  90% of sequence in fragments shorter than  230244 bp
  95% of sequence in fragments shorter than  342598 bp

# PvuI.SnaBI (CGATCG+TACGTA) double digest, 146 thousand fragments (14186+132299 sites), 20388 bp average fragment length
   5% of sequence in fragments shorter than    7282 bp
  10% of sequence in fragments shorter than   11261 bp
  15% of sequence in fragments shorter than   14654 bp
  20% of sequence in fragments shorter than   17818 bp
  25% of sequence in fragments shorter than   20967 bp
  30% of sequence in fragments shorter than   24142 bp
  35% of sequence in fragments shorter than   27392 bp
  40% of sequence in fragments shorter than   30666 bp
  45% of sequence in fragments shorter than   34028 bp
  50% of sequence in fragments shorter than   37713 bp
  55% of sequence in fragments shorter than   41655 bp
  60% of sequence in fragments shorter than   45933 bp
  65% of sequence in fragments shorter than   50669 bp
  70% of sequence in fragments shorter than   56136 bp
  75% of sequence in fragments shorter than   62619 bp
  80% of sequence in fragments shorter than   70644 bp
  85% of sequence in fragments shorter than   81136 bp
  90% of sequence in fragments shorter than   96216 bp
  95% of sequence in fragments shorter than  125125 bp

# FseI.SnaBI (GGCCGGCC+TACGTA) double digest, 145 thousand fragments (13662+132299 sites), 20459 bp average fragment length
   5% of sequence in fragments shorter than    7360 bp
  10% of sequence in fragments shorter than   11295 bp
  15% of sequence in fragments shorter than   14655 bp
  20% of sequence in fragments shorter than   17790 bp
  25% of sequence in fragments shorter than   20945 bp
  30% of sequence in fragments shorter than   24069 bp
  35% of sequence in fragments shorter than   27287 bp
  40% of sequence in fragments shorter than   30549 bp
  45% of sequence in fragments shorter than   34003 bp
  50% of sequence in fragments shorter than   37651 bp
  55% of sequence in fragments shorter than   41489 bp
  60% of sequence in fragments shorter than   45778 bp
  65% of sequence in fragments shorter than   50510 bp
  70% of sequence in fragments shorter than   55937 bp
  75% of sequence in fragments shorter than   62332 bp
  80% of sequence in fragments shorter than   70201 bp
  85% of sequence in fragments shorter than   80465 bp
  90% of sequence in fragments shorter than   95592 bp
  95% of sequence in fragments shorter than  126063 bp

# ClaI.PspXI (ATCGAT+VCTCGAGB) double digest, 145 thousand fragments (86477+59193 sites), 20500 bp average fragment length
   5% of sequence in fragments shorter than    7128 bp
  10% of sequence in fragments shorter than   10822 bp
  15% of sequence in fragments shorter than   14159 bp
  20% of sequence in fragments shorter than   17152 bp
  25% of sequence in fragments shorter than   20219 bp
  30% of sequence in fragments shorter than   23202 bp
  35% of sequence in fragments shorter than   26234 bp
  40% of sequence in fragments shorter than   29350 bp
  45% of sequence in fragments shorter than   32758 bp
  50% of sequence in fragments shorter than   36376 bp
  55% of sequence in fragments shorter than   40213 bp
  60% of sequence in fragments shorter than   44471 bp
  65% of sequence in fragments shorter than   49168 bp
  70% of sequence in fragments shorter than   54510 bp
  75% of sequence in fragments shorter than   60586 bp
  80% of sequence in fragments shorter than   68179 bp
  85% of sequence in fragments shorter than   78321 bp
  90% of sequence in fragments shorter than   92902 bp
  95% of sequence in fragments shorter than  122649 bp

# MluI.XhoI (ACGCGT+CTCGAG) double digest, 142 thousand fragments (21415+121308 sites), 20925 bp average fragment length
   5% of sequence in fragments shorter than    7127 bp
  10% of sequence in fragments shorter than   11000 bp
  15% of sequence in fragments shorter than   14540 bp
  20% of sequence in fragments shorter than   17924 bp
  25% of sequence in fragments shorter than   21390 bp
  30% of sequence in fragments shorter than   24937 bp
  35% of sequence in fragments shorter than   28496 bp
  40% of sequence in fragments shorter than   32389 bp
  45% of sequence in fragments shorter than   36548 bp
  50% of sequence in fragments shorter than   40923 bp
  55% of sequence in fragments shorter than   45887 bp
  60% of sequence in fragments shorter than   51319 bp
  65% of sequence in fragments shorter than   57601 bp
  70% of sequence in fragments shorter than   64576 bp
  75% of sequence in fragments shorter than   73218 bp
  80% of sequence in fragments shorter than   83543 bp
  85% of sequence in fragments shorter than   97819 bp
  90% of sequence in fragments shorter than  118953 bp
  95% of sequence in fragments shorter than  161922 bp

# NotI.SnaBI (GCGGCCGC+TACGTA) double digest, 141 thousand fragments (9795+132299 sites), 21017 bp average fragment length
   5% of sequence in fragments shorter than    7573 bp
  10% of sequence in fragments shorter than   11667 bp
  15% of sequence in fragments shorter than   15175 bp
  20% of sequence in fragments shorter than   18455 bp
  25% of sequence in fragments shorter than   21759 bp
  30% of sequence in fragments shorter than   25026 bp
  35% of sequence in fragments shorter than   28334 bp
  40% of sequence in fragments shorter than   31672 bp
  45% of sequence in fragments shorter than   35224 bp
  50% of sequence in fragments shorter than   39090 bp
  55% of sequence in fragments shorter than   43162 bp
  60% of sequence in fragments shorter than   47564 bp
  65% of sequence in fragments shorter than   52481 bp
  70% of sequence in fragments shorter than   58030 bp
  75% of sequence in fragments shorter than   64762 bp
  80% of sequence in fragments shorter than   73348 bp
  85% of sequence in fragments shorter than   84266 bp
  90% of sequence in fragments shorter than  100163 bp
  95% of sequence in fragments shorter than  134779 bp

# RsrII.SnaBI (CGGWCCG+TACGTA) double digest, 141 thousand fragments (9764+132299 sites), 21022 bp average fragment length
   5% of sequence in fragments shorter than    7548 bp
  10% of sequence in fragments shorter than   11610 bp
  15% of sequence in fragments shorter than   15080 bp
  20% of sequence in fragments shorter than   18339 bp
  25% of sequence in fragments shorter than   21599 bp
  30% of sequence in fragments shorter than   24839 bp
  35% of sequence in fragments shorter than   28100 bp
  40% of sequence in fragments shorter than   31405 bp
  45% of sequence in fragments shorter than   34957 bp
  50% of sequence in fragments shorter than   38744 bp
  55% of sequence in fragments shorter than   42740 bp
  60% of sequence in fragments shorter than   47128 bp
  65% of sequence in fragments shorter than   52042 bp
  70% of sequence in fragments shorter than   57571 bp
  75% of sequence in fragments shorter than   64164 bp
  80% of sequence in fragments shorter than   72525 bp
  85% of sequence in fragments shorter than   83132 bp
  90% of sequence in fragments shorter than   98784 bp
  95% of sequence in fragments shorter than  131530 bp

# NaeI.SgrAI (GCCGGC+CRCCGGYG) double digest, 140 thousand fragments (125692+15270 sites), 21173 bp average fragment length
   5% of sequence in fragments shorter than    7708 bp
  10% of sequence in fragments shorter than   13443 bp
  15% of sequence in fragments shorter than   19185 bp
  20% of sequence in fragments shorter than   25102 bp
  25% of sequence in fragments shorter than   31343 bp
  30% of sequence in fragments shorter than   37959 bp
  35% of sequence in fragments shorter than   45111 bp
  40% of sequence in fragments shorter than   53000 bp
  45% of sequence in fragments shorter than   61496 bp
  50% of sequence in fragments shorter than   71075 bp
  55% of sequence in fragments shorter than   81610 bp
  60% of sequence in fragments shorter than   94127 bp
  65% of sequence in fragments shorter than  108201 bp
  70% of sequence in fragments shorter than  124938 bp
  75% of sequence in fragments shorter than  146087 bp
  80% of sequence in fragments shorter than  171666 bp
  85% of sequence in fragments shorter than  207747 bp
  90% of sequence in fragments shorter than  267407 bp
  95% of sequence in fragments shorter than  407806 bp

# NaeI.NruI (GCCGGC+TCGCGA) double digest, 140 thousand fragments (125692+14872 sites), 21236 bp average fragment length
   5% of sequence in fragments shorter than    7468 bp
  10% of sequence in fragments shorter than   12907 bp
  15% of sequence in fragments shorter than   18313 bp
  20% of sequence in fragments shorter than   23908 bp
  25% of sequence in fragments shorter than   29772 bp
  30% of sequence in fragments shorter than   36074 bp
  35% of sequence in fragments shorter than   42795 bp
  40% of sequence in fragments shorter than   49897 bp
  45% of sequence in fragments shorter than   57777 bp
  50% of sequence in fragments shorter than   66585 bp
  55% of sequence in fragments shorter than   76552 bp
  60% of sequence in fragments shorter than   87531 bp
  65% of sequence in fragments shorter than  100334 bp
  70% of sequence in fragments shorter than  115728 bp
  75% of sequence in fragments shorter than  135304 bp
  80% of sequence in fragments shorter than  158857 bp
  85% of sequence in fragments shorter than  190633 bp
  90% of sequence in fragments shorter than  242854 bp
  95% of sequence in fragments shorter than  368615 bp

# NaeI.PvuI (GCCGGC+CGATCG) double digest, 139 thousand fragments (125692+14186 sites), 21342 bp average fragment length
   5% of sequence in fragments shorter than    7432 bp
  10% of sequence in fragments shorter than   12944 bp
  15% of sequence in fragments shorter than   18436 bp
  20% of sequence in fragments shorter than   24014 bp
  25% of sequence in fragments shorter than   29915 bp
  30% of sequence in fragments shorter than   36199 bp
  35% of sequence in fragments shorter than   42917 bp
  40% of sequence in fragments shorter than   50090 bp
  45% of sequence in fragments shorter than   58017 bp
  50% of sequence in fragments shorter than   66756 bp
  55% of sequence in fragments shorter than   76699 bp
  60% of sequence in fragments shorter than   88271 bp
  65% of sequence in fragments shorter than  101609 bp
  70% of sequence in fragments shorter than  117038 bp
  75% of sequence in fragments shorter than  135966 bp
  80% of sequence in fragments shorter than  158956 bp
  85% of sequence in fragments shorter than  191594 bp
  90% of sequence in fragments shorter than  241563 bp
  95% of sequence in fragments shorter than  362451 bp

# AgeI.ClaI (ACCGGT+ATCGAT) double digest, 139 thousand fragments (53386+86477 sites), 21353 bp average fragment length
   5% of sequence in fragments shorter than    7564 bp
  10% of sequence in fragments shorter than   11407 bp
  15% of sequence in fragments shorter than   14759 bp
  20% of sequence in fragments shorter than   17887 bp
  25% of sequence in fragments shorter than   20962 bp
  30% of sequence in fragments shorter than   24031 bp
  35% of sequence in fragments shorter than   27166 bp
  40% of sequence in fragments shorter than   30314 bp
  45% of sequence in fragments shorter than   33750 bp
  50% of sequence in fragments shorter than   37293 bp
  55% of sequence in fragments shorter than   41043 bp
  60% of sequence in fragments shorter than   45310 bp
  65% of sequence in fragments shorter than   49886 bp
  70% of sequence in fragments shorter than   55013 bp
  75% of sequence in fragments shorter than   61112 bp
  80% of sequence in fragments shorter than   68549 bp
  85% of sequence in fragments shorter than   78279 bp
  90% of sequence in fragments shorter than   92758 bp
  95% of sequence in fragments shorter than  122373 bp

# FseI.NaeI (GGCCGGCC+GCCGGC) double digest, 139 thousand fragments (13662+125692 sites), 21418 bp average fragment length
   5% of sequence in fragments shorter than    8307 bp
  10% of sequence in fragments shorter than   14500 bp
  15% of sequence in fragments shorter than   20658 bp
  20% of sequence in fragments shorter than   27131 bp
  25% of sequence in fragments shorter than   33918 bp
  30% of sequence in fragments shorter than   41127 bp
  35% of sequence in fragments shorter than   48915 bp
  40% of sequence in fragments shorter than   57273 bp
  45% of sequence in fragments shorter than   66678 bp
  50% of sequence in fragments shorter than   77259 bp
  55% of sequence in fragments shorter than   88936 bp
  60% of sequence in fragments shorter than  103023 bp
  65% of sequence in fragments shorter than  117967 bp
  70% of sequence in fragments shorter than  137093 bp
  75% of sequence in fragments shorter than  159464 bp
  80% of sequence in fragments shorter than  188342 bp
  85% of sequence in fragments shorter than  229135 bp
  90% of sequence in fragments shorter than  299492 bp
  95% of sequence in fragments shorter than  458841 bp

# AatII.SacII (GACGTC+CCGCGG) double digest, 137 thousand fragments (71089+66901 sites), 21643 bp average fragment length
   5% of sequence in fragments shorter than    8452 bp
  10% of sequence in fragments shorter than   13987 bp
  15% of sequence in fragments shorter than   19203 bp
  20% of sequence in fragments shorter than   24327 bp
  25% of sequence in fragments shorter than   29597 bp
  30% of sequence in fragments shorter than   35037 bp
  35% of sequence in fragments shorter than   40723 bp
  40% of sequence in fragments shorter than   46681 bp
  45% of sequence in fragments shorter than   53127 bp
  50% of sequence in fragments shorter than   60198 bp
  55% of sequence in fragments shorter than   67678 bp
  60% of sequence in fragments shorter than   76157 bp
  65% of sequence in fragments shorter than   85847 bp
  70% of sequence in fragments shorter than   96788 bp
  75% of sequence in fragments shorter than  110044 bp
  80% of sequence in fragments shorter than  126888 bp
  85% of sequence in fragments shorter than  148625 bp
  90% of sequence in fragments shorter than  184261 bp
  95% of sequence in fragments shorter than  247294 bp

# AscI.SnaBI (GGCGCGCC+TACGTA) double digest, 136 thousand fragments (4751+132299 sites), 21793 bp average fragment length
   5% of sequence in fragments shorter than    7811 bp
  10% of sequence in fragments shorter than   12014 bp
  15% of sequence in fragments shorter than   15625 bp
  20% of sequence in fragments shorter than   18997 bp
  25% of sequence in fragments shorter than   22402 bp
  30% of sequence in fragments shorter than   25754 bp
  35% of sequence in fragments shorter than   29196 bp
  40% of sequence in fragments shorter than   32665 bp
  45% of sequence in fragments shorter than   36370 bp
  50% of sequence in fragments shorter than   40380 bp
  55% of sequence in fragments shorter than   44737 bp
  60% of sequence in fragments shorter than   49194 bp
  65% of sequence in fragments shorter than   54448 bp
  70% of sequence in fragments shorter than   60312 bp
  75% of sequence in fragments shorter than   67639 bp
  80% of sequence in fragments shorter than   76631 bp
  85% of sequence in fragments shorter than   88039 bp
  90% of sequence in fragments shorter than  105270 bp
  95% of sequence in fragments shorter than  142252 bp

# SgrAI.XhoI (CRCCGGYG+CTCGAG) double digest, 136 thousand fragments (15270+121308 sites), 21865 bp average fragment length
   5% of sequence in fragments shorter than    7487 bp
  10% of sequence in fragments shorter than   11583 bp
  15% of sequence in fragments shorter than   15306 bp
  20% of sequence in fragments shorter than   18886 bp
  25% of sequence in fragments shorter than   22482 bp
  30% of sequence in fragments shorter than   26188 bp
  35% of sequence in fragments shorter than   29999 bp
  40% of sequence in fragments shorter than   34147 bp
  45% of sequence in fragments shorter than   38476 bp
  50% of sequence in fragments shorter than   43239 bp
  55% of sequence in fragments shorter than   48379 bp
  60% of sequence in fragments shorter than   54128 bp
  65% of sequence in fragments shorter than   60654 bp
  70% of sequence in fragments shorter than   68163 bp
  75% of sequence in fragments shorter than   77217 bp
  80% of sequence in fragments shorter than   88033 bp
  85% of sequence in fragments shorter than  102818 bp
  90% of sequence in fragments shorter than  125698 bp
  95% of sequence in fragments shorter than  172354 bp

# NruI.XhoI (TCGCGA+CTCGAG) double digest, 135 thousand fragments (14872+121308 sites), 21932 bp average fragment length
   5% of sequence in fragments shorter than    7486 bp
  10% of sequence in fragments shorter than   11495 bp
  15% of sequence in fragments shorter than   15141 bp
  20% of sequence in fragments shorter than   18666 bp
  25% of sequence in fragments shorter than   22149 bp
  30% of sequence in fragments shorter than   25809 bp
  35% of sequence in fragments shorter than   29575 bp
  40% of sequence in fragments shorter than   33695 bp
  45% of sequence in fragments shorter than   37899 bp
  50% of sequence in fragments shorter than   42548 bp
  55% of sequence in fragments shorter than   47519 bp
  60% of sequence in fragments shorter than   53208 bp
  65% of sequence in fragments shorter than   59593 bp
  70% of sequence in fragments shorter than   66941 bp
  75% of sequence in fragments shorter than   75503 bp
  80% of sequence in fragments shorter than   86414 bp
  85% of sequence in fragments shorter than  100295 bp
  90% of sequence in fragments shorter than  122619 bp
  95% of sequence in fragments shorter than  168471 bp

# NaeI.NotI (GCCGGC+GCGGCCGC) double digest, 135 thousand fragments (125692+9795 sites), 22030 bp average fragment length
   5% of sequence in fragments shorter than    7960 bp
  10% of sequence in fragments shorter than   13968 bp
  15% of sequence in fragments shorter than   19997 bp
  20% of sequence in fragments shorter than   26288 bp
  25% of sequence in fragments shorter than   32882 bp
  30% of sequence in fragments shorter than   39900 bp
  35% of sequence in fragments shorter than   47528 bp
  40% of sequence in fragments shorter than   55733 bp
  45% of sequence in fragments shorter than   64789 bp
  50% of sequence in fragments shorter than   75217 bp
  55% of sequence in fragments shorter than   86798 bp
  60% of sequence in fragments shorter than  100224 bp
  65% of sequence in fragments shorter than  115102 bp
  70% of sequence in fragments shorter than  134065 bp
  75% of sequence in fragments shorter than  156212 bp
  80% of sequence in fragments shorter than  184815 bp
  85% of sequence in fragments shorter than  225421 bp
  90% of sequence in fragments shorter than  293522 bp
  95% of sequence in fragments shorter than  456525 bp

# NaeI.RsrII (GCCGGC+CGGWCCG) double digest, 135 thousand fragments (125692+9764 sites), 22038 bp average fragment length
   5% of sequence in fragments shorter than    7810 bp
  10% of sequence in fragments shorter than   13701 bp
  15% of sequence in fragments shorter than   19546 bp
  20% of sequence in fragments shorter than   25670 bp
  25% of sequence in fragments shorter than   32120 bp
  30% of sequence in fragments shorter than   38886 bp
  35% of sequence in fragments shorter than   46185 bp
  40% of sequence in fragments shorter than   54208 bp
  45% of sequence in fragments shorter than   62935 bp
  50% of sequence in fragments shorter than   72969 bp
  55% of sequence in fragments shorter than   83823 bp
  60% of sequence in fragments shorter than   96950 bp
  65% of sequence in fragments shorter than  111704 bp
  70% of sequence in fragments shorter than  129206 bp
  75% of sequence in fragments shorter than  151070 bp
  80% of sequence in fragments shorter than  178378 bp
  85% of sequence in fragments shorter than  215529 bp
  90% of sequence in fragments shorter than  278825 bp
  95% of sequence in fragments shorter than  425008 bp

# PvuI.XhoI (CGATCG+CTCGAG) double digest, 135 thousand fragments (14186+121308 sites), 22043 bp average fragment length
   5% of sequence in fragments shorter than    7506 bp
  10% of sequence in fragments shorter than   11605 bp
  15% of sequence in fragments shorter than   15318 bp
  20% of sequence in fragments shorter than   18867 bp
  25% of sequence in fragments shorter than   22426 bp
  30% of sequence in fragments shorter than   26069 bp
  35% of sequence in fragments shorter than   29863 bp
  40% of sequence in fragments shorter than   34019 bp
  45% of sequence in fragments shorter than   38242 bp
  50% of sequence in fragments shorter than   42930 bp
  55% of sequence in fragments shorter than   47973 bp
  60% of sequence in fragments shorter than   53454 bp
  65% of sequence in fragments shorter than   60030 bp
  70% of sequence in fragments shorter than   67295 bp
  75% of sequence in fragments shorter than   75801 bp
  80% of sequence in fragments shorter than   86441 bp
  85% of sequence in fragments shorter than  100599 bp
  90% of sequence in fragments shorter than  122529 bp
  95% of sequence in fragments shorter than  166563 bp

# FseI.XhoI (GGCCGGCC+CTCGAG) double digest, 134 thousand fragments (13662+121308 sites), 22126 bp average fragment length
   5% of sequence in fragments shorter than    7496 bp
  10% of sequence in fragments shorter than   11616 bp
  15% of sequence in fragments shorter than   15375 bp
  20% of sequence in fragments shorter than   19023 bp
  25% of sequence in fragments shorter than   22673 bp
  30% of sequence in fragments shorter than   26486 bp
  35% of sequence in fragments shorter than   30450 bp
  40% of sequence in fragments shorter than   34731 bp
  45% of sequence in fragments shorter than   39175 bp
  50% of sequence in fragments shorter than   44227 bp
  55% of sequence in fragments shorter than   49476 bp
  60% of sequence in fragments shorter than   55343 bp
  65% of sequence in fragments shorter than   62137 bp
  70% of sequence in fragments shorter than   69785 bp
  75% of sequence in fragments shorter than   78956 bp
  80% of sequence in fragments shorter than   90712 bp
  85% of sequence in fragments shorter than  106403 bp
  90% of sequence in fragments shorter than  129282 bp
  95% of sequence in fragments shorter than  176864 bp

# NotI.XhoI (GCGGCCGC+CTCGAG) double digest, 130 thousand fragments (9795+121308 sites), 22780 bp average fragment length
   5% of sequence in fragments shorter than    7829 bp
  10% of sequence in fragments shorter than   12113 bp
  15% of sequence in fragments shorter than   16015 bp
  20% of sequence in fragments shorter than   19775 bp
  25% of sequence in fragments shorter than   23534 bp
  30% of sequence in fragments shorter than   27352 bp
  35% of sequence in fragments shorter than   31454 bp
  40% of sequence in fragments shorter than   35784 bp
  45% of sequence in fragments shorter than   40336 bp
  50% of sequence in fragments shorter than   45373 bp
  55% of sequence in fragments shorter than   50752 bp
  60% of sequence in fragments shorter than   56760 bp
  65% of sequence in fragments shorter than   63543 bp
  70% of sequence in fragments shorter than   71440 bp
  75% of sequence in fragments shorter than   80828 bp
  80% of sequence in fragments shorter than   92514 bp
  85% of sequence in fragments shorter than  108377 bp
  90% of sequence in fragments shorter than  131929 bp
  95% of sequence in fragments shorter than  181330 bp

# RsrII.XhoI (CGGWCCG+CTCGAG) double digest, 130 thousand fragments (9764+121308 sites), 22788 bp average fragment length
   5% of sequence in fragments shorter than    7763 bp
  10% of sequence in fragments shorter than   11999 bp
  15% of sequence in fragments shorter than   15842 bp
  20% of sequence in fragments shorter than   19535 bp
  25% of sequence in fragments shorter than   23238 bp
  30% of sequence in fragments shorter than   27061 bp
  35% of sequence in fragments shorter than   31077 bp
  40% of sequence in fragments shorter than   35371 bp
  45% of sequence in fragments shorter than   39882 bp
  50% of sequence in fragments shorter than   44852 bp
  55% of sequence in fragments shorter than   50198 bp
  60% of sequence in fragments shorter than   56068 bp
  65% of sequence in fragments shorter than   62820 bp
  70% of sequence in fragments shorter than   70640 bp
  75% of sequence in fragments shorter than   79784 bp
  80% of sequence in fragments shorter than   91381 bp
  85% of sequence in fragments shorter than  106744 bp
  90% of sequence in fragments shorter than  129788 bp
  95% of sequence in fragments shorter than  178293 bp

# AscI.NaeI (GGCGCGCC+GCCGGC) double digest, 130 thousand fragments (4751+125692 sites), 22884 bp average fragment length
   5% of sequence in fragments shorter than    8135 bp
  10% of sequence in fragments shorter than   14223 bp
  15% of sequence in fragments shorter than   20266 bp
  20% of sequence in fragments shorter than   26605 bp
  25% of sequence in fragments shorter than   33294 bp
  30% of sequence in fragments shorter than   40394 bp
  35% of sequence in fragments shorter than   48136 bp
  40% of sequence in fragments shorter than   56340 bp
  45% of sequence in fragments shorter than   65610 bp
  50% of sequence in fragments shorter than   76022 bp
  55% of sequence in fragments shorter than   87505 bp
  60% of sequence in fragments shorter than  101264 bp
  65% of sequence in fragments shorter than  116340 bp
  70% of sequence in fragments shorter than  135265 bp
  75% of sequence in fragments shorter than  157626 bp
  80% of sequence in fragments shorter than  186397 bp
  85% of sequence in fragments shorter than  226495 bp
  90% of sequence in fragments shorter than  293371 bp
  95% of sequence in fragments shorter than  454228 bp

# AatII.PspXI (GACGTC+VCTCGAGB) double digest, 129 thousand fragments (71089+59193 sites), 22930 bp average fragment length
   5% of sequence in fragments shorter than    7585 bp
  10% of sequence in fragments shorter than   11916 bp
  15% of sequence in fragments shorter than   15894 bp
  20% of sequence in fragments shorter than   19789 bp
  25% of sequence in fragments shorter than   23722 bp
  30% of sequence in fragments shorter than   27756 bp
  35% of sequence in fragments shorter than   31886 bp
  40% of sequence in fragments shorter than   36414 bp
  45% of sequence in fragments shorter than   41134 bp
  50% of sequence in fragments shorter than   46344 bp
  55% of sequence in fragments shorter than   52051 bp
  60% of sequence in fragments shorter than   58342 bp
  65% of sequence in fragments shorter than   65223 bp
  70% of sequence in fragments shorter than   73201 bp
  75% of sequence in fragments shorter than   82879 bp
  80% of sequence in fragments shorter than   95329 bp
  85% of sequence in fragments shorter than  110787 bp
  90% of sequence in fragments shorter than  133814 bp
  95% of sequence in fragments shorter than  181858 bp

# ClaI.PmeI (ATCGAT+GTTTAAAC) double digest, 128 thousand fragments (86477+42094 sites), 23235 bp average fragment length
   5% of sequence in fragments shorter than    8187 bp
  10% of sequence in fragments shorter than   12350 bp
  15% of sequence in fragments shorter than   15983 bp
  20% of sequence in fragments shorter than   19411 bp
  25% of sequence in fragments shorter than   22704 bp
  30% of sequence in fragments shorter than   25946 bp
  35% of sequence in fragments shorter than   29300 bp
  40% of sequence in fragments shorter than   32704 bp
  45% of sequence in fragments shorter than   36384 bp
  50% of sequence in fragments shorter than   40157 bp
  55% of sequence in fragments shorter than   44288 bp
  60% of sequence in fragments shorter than   48952 bp
  65% of sequence in fragments shorter than   54095 bp
  70% of sequence in fragments shorter than   59707 bp
  75% of sequence in fragments shorter than   66337 bp
  80% of sequence in fragments shorter than   74473 bp
  85% of sequence in fragments shorter than   85085 bp
  90% of sequence in fragments shorter than  101391 bp
  95% of sequence in fragments shorter than  133115 bp

# PspXI.SacII (VCTCGAGB+CCGCGG) double digest, 125 thousand fragments (59193+66901 sites), 23683 bp average fragment length
   5% of sequence in fragments shorter than    9195 bp
  10% of sequence in fragments shorter than   15353 bp
  15% of sequence in fragments shorter than   21290 bp
  20% of sequence in fragments shorter than   27192 bp
  25% of sequence in fragments shorter than   33330 bp
  30% of sequence in fragments shorter than   39657 bp
  35% of sequence in fragments shorter than   46579 bp
  40% of sequence in fragments shorter than   54101 bp
  45% of sequence in fragments shorter than   61859 bp
  50% of sequence in fragments shorter than   70284 bp
  55% of sequence in fragments shorter than   79767 bp
  60% of sequence in fragments shorter than   90938 bp
  65% of sequence in fragments shorter than  102852 bp
  70% of sequence in fragments shorter than  118333 bp
  75% of sequence in fragments shorter than  135614 bp
  80% of sequence in fragments shorter than  158056 bp
  85% of sequence in fragments shorter than  189291 bp
  90% of sequence in fragments shorter than  236315 bp
  95% of sequence in fragments shorter than  337316 bp

# AscI.XhoI (GGCGCGCC+CTCGAG) double digest, 125 thousand fragments (4751+121308 sites), 23695 bp average fragment length
   5% of sequence in fragments shorter than    8098 bp
  10% of sequence in fragments shorter than   12463 bp
  15% of sequence in fragments shorter than   16406 bp
  20% of sequence in fragments shorter than   20215 bp
  25% of sequence in fragments shorter than   24032 bp
  30% of sequence in fragments shorter than   27876 bp
  35% of sequence in fragments shorter than   32047 bp
  40% of sequence in fragments shorter than   36428 bp
  45% of sequence in fragments shorter than   40980 bp
  50% of sequence in fragments shorter than   46064 bp
  55% of sequence in fragments shorter than   51474 bp
  60% of sequence in fragments shorter than   57568 bp
  65% of sequence in fragments shorter than   64429 bp
  70% of sequence in fragments shorter than   72442 bp
  75% of sequence in fragments shorter than   81767 bp
  80% of sequence in fragments shorter than   93720 bp
  85% of sequence in fragments shorter than  109354 bp
  90% of sequence in fragments shorter than  132791 bp
  95% of sequence in fragments shorter than  182103 bp

# AatII.AgeI (GACGTC+ACCGGT) double digest, 124 thousand fragments (71089+53386 sites), 24000 bp average fragment length
   5% of sequence in fragments shorter than    8182 bp
  10% of sequence in fragments shorter than   12672 bp
  15% of sequence in fragments shorter than   16623 bp
  20% of sequence in fragments shorter than   20395 bp
  25% of sequence in fragments shorter than   24210 bp
  30% of sequence in fragments shorter than   28005 bp
  35% of sequence in fragments shorter than   31964 bp
  40% of sequence in fragments shorter than   36135 bp
  45% of sequence in fragments shorter than   40574 bp
  50% of sequence in fragments shorter than   45375 bp
  55% of sequence in fragments shorter than   50687 bp
  60% of sequence in fragments shorter than   56276 bp
  65% of sequence in fragments shorter than   62716 bp
  70% of sequence in fragments shorter than   70107 bp
  75% of sequence in fragments shorter than   79018 bp
  80% of sequence in fragments shorter than   90354 bp
  85% of sequence in fragments shorter than  104444 bp
  90% of sequence in fragments shorter than  124210 bp
  95% of sequence in fragments shorter than  165266 bp

# AgeI.SacII (ACCGGT+CCGCGG) double digest, 120 thousand fragments (53386+66901 sites), 24828 bp average fragment length
   5% of sequence in fragments shorter than   10154 bp
  10% of sequence in fragments shorter than   16610 bp
  15% of sequence in fragments shorter than   22434 bp
  20% of sequence in fragments shorter than   28131 bp
  25% of sequence in fragments shorter than   33946 bp
  30% of sequence in fragments shorter than   39798 bp
  35% of sequence in fragments shorter than   45952 bp
  40% of sequence in fragments shorter than   52482 bp
  45% of sequence in fragments shorter than   59334 bp
  50% of sequence in fragments shorter than   66870 bp
  55% of sequence in fragments shorter than   74861 bp
  60% of sequence in fragments shorter than   84123 bp
  65% of sequence in fragments shorter than   94511 bp
  70% of sequence in fragments shorter than  105955 bp
  75% of sequence in fragments shorter than  119889 bp
  80% of sequence in fragments shorter than  136699 bp
  85% of sequence in fragments shorter than  159927 bp
  90% of sequence in fragments shorter than  195920 bp
  95% of sequence in fragments shorter than  276518 bp

# ClaI.SalI (ATCGAT+GTCGAC) double digest, 117 thousand fragments (86477+31396 sites), 25348 bp average fragment length
   5% of sequence in fragments shorter than    8977 bp
  10% of sequence in fragments shorter than   13586 bp
  15% of sequence in fragments shorter than   17542 bp
  20% of sequence in fragments shorter than   21245 bp
  25% of sequence in fragments shorter than   24886 bp
  30% of sequence in fragments shorter than   28495 bp
  35% of sequence in fragments shorter than   32223 bp
  40% of sequence in fragments shorter than   36087 bp
  45% of sequence in fragments shorter than   40215 bp
  50% of sequence in fragments shorter than   44453 bp
  55% of sequence in fragments shorter than   49035 bp
  60% of sequence in fragments shorter than   53794 bp
  65% of sequence in fragments shorter than   59290 bp
  70% of sequence in fragments shorter than   65661 bp
  75% of sequence in fragments shorter than   72780 bp
  80% of sequence in fragments shorter than   81679 bp
  85% of sequence in fragments shorter than   93950 bp
  90% of sequence in fragments shorter than  111344 bp
  95% of sequence in fragments shorter than  145892 bp

# AatII.PmeI (GACGTC+GTTTAAAC) double digest, 112 thousand fragments (71089+42094 sites), 26405 bp average fragment length
   5% of sequence in fragments shorter than    9217 bp
  10% of sequence in fragments shorter than   14070 bp
  15% of sequence in fragments shorter than   18240 bp
  20% of sequence in fragments shorter than   22272 bp
  25% of sequence in fragments shorter than   26190 bp
  30% of sequence in fragments shorter than   30102 bp
  35% of sequence in fragments shorter than   34136 bp
  40% of sequence in fragments shorter than   38269 bp
  45% of sequence in fragments shorter than   42700 bp
  50% of sequence in fragments shorter than   47429 bp
  55% of sequence in fragments shorter than   52423 bp
  60% of sequence in fragments shorter than   57831 bp
  65% of sequence in fragments shorter than   63805 bp
  70% of sequence in fragments shorter than   70780 bp
  75% of sequence in fragments shorter than   78773 bp
  80% of sequence in fragments shorter than   88689 bp
  85% of sequence in fragments shorter than  101193 bp
  90% of sequence in fragments shorter than  119897 bp
  95% of sequence in fragments shorter than  156829 bp

# AgeI.PspXI (ACCGGT+VCTCGAGB) double digest, 112 thousand fragments (53386+59193 sites), 26537 bp average fragment length
   5% of sequence in fragments shorter than    8894 bp
  10% of sequence in fragments shorter than   13849 bp
  15% of sequence in fragments shorter than   18224 bp
  20% of sequence in fragments shorter than   22389 bp
  25% of sequence in fragments shorter than   26695 bp
  30% of sequence in fragments shorter than   30991 bp
  35% of sequence in fragments shorter than   35547 bp
  40% of sequence in fragments shorter than   40116 bp
  45% of sequence in fragments shorter than   45145 bp
  50% of sequence in fragments shorter than   50630 bp
  55% of sequence in fragments shorter than   56490 bp
  60% of sequence in fragments shorter than   62769 bp
  65% of sequence in fragments shorter than   69889 bp
  70% of sequence in fragments shorter than   78214 bp
  75% of sequence in fragments shorter than   88293 bp
  80% of sequence in fragments shorter than  100249 bp
  85% of sequence in fragments shorter than  117628 bp
  90% of sequence in fragments shorter than  143417 bp
  95% of sequence in fragments shorter than  201233 bp

# PmeI.SacII (GTTTAAAC+CCGCGG) double digest, 108 thousand fragments (42094+66901 sites), 27412 bp average fragment length
   5% of sequence in fragments shorter than   11684 bp
  10% of sequence in fragments shorter than   18708 bp
  15% of sequence in fragments shorter than   24682 bp
  20% of sequence in fragments shorter than   30555 bp
  25% of sequence in fragments shorter than   36361 bp
  30% of sequence in fragments shorter than   41997 bp
  35% of sequence in fragments shorter than   48254 bp
  40% of sequence in fragments shorter than   54574 bp
  45% of sequence in fragments shorter than   61459 bp
  50% of sequence in fragments shorter than   68368 bp
  55% of sequence in fragments shorter than   76058 bp
  60% of sequence in fragments shorter than   84246 bp
  65% of sequence in fragments shorter than   93399 bp
  70% of sequence in fragments shorter than  104176 bp
  75% of sequence in fragments shorter than  116536 bp
  80% of sequence in fragments shorter than  132118 bp
  85% of sequence in fragments shorter than  152805 bp
  90% of sequence in fragments shorter than  181968 bp
  95% of sequence in fragments shorter than  246507 bp

# ClaI.MluI (ATCGAT+ACGCGT) double digest, 107 thousand fragments (86477+21415 sites), 27697 bp average fragment length
   5% of sequence in fragments shorter than    9829 bp
  10% of sequence in fragments shorter than   14887 bp
  15% of sequence in fragments shorter than   19301 bp
  20% of sequence in fragments shorter than   23352 bp
  25% of sequence in fragments shorter than   27345 bp
  30% of sequence in fragments shorter than   31372 bp
  35% of sequence in fragments shorter than   35577 bp
  40% of sequence in fragments shorter than   39822 bp
  45% of sequence in fragments shorter than   44257 bp
  50% of sequence in fragments shorter than   49043 bp
  55% of sequence in fragments shorter than   53974 bp
  60% of sequence in fragments shorter than   59501 bp
  65% of sequence in fragments shorter than   65901 bp
  70% of sequence in fragments shorter than   73069 bp
  75% of sequence in fragments shorter than   81306 bp
  80% of sequence in fragments shorter than   91382 bp
  85% of sequence in fragments shorter than  104945 bp
  90% of sequence in fragments shorter than  123722 bp
  95% of sequence in fragments shorter than  163545 bp

# AatII.SalI (GACGTC+GTCGAC) double digest, 102 thousand fragments (71089+31396 sites), 29169 bp average fragment length
   5% of sequence in fragments shorter than    9979 bp
  10% of sequence in fragments shorter than   15467 bp
  15% of sequence in fragments shorter than   20423 bp
  20% of sequence in fragments shorter than   25114 bp
  25% of sequence in fragments shorter than   29947 bp
  30% of sequence in fragments shorter than   34687 bp
  35% of sequence in fragments shorter than   39682 bp
  40% of sequence in fragments shorter than   44876 bp
  45% of sequence in fragments shorter than   50572 bp
  50% of sequence in fragments shorter than   56379 bp
  55% of sequence in fragments shorter than   62861 bp
  60% of sequence in fragments shorter than   69680 bp
  65% of sequence in fragments shorter than   77920 bp
  70% of sequence in fragments shorter than   87124 bp
  75% of sequence in fragments shorter than   97560 bp
  80% of sequence in fragments shorter than  109928 bp
  85% of sequence in fragments shorter than  127722 bp
  90% of sequence in fragments shorter than  155071 bp
  95% of sequence in fragments shorter than  210163 bp

# ClaI.SgrAI (ATCGAT+CRCCGGYG) double digest, 101 thousand fragments (86477+15270 sites), 29370 bp average fragment length
   5% of sequence in fragments shorter than   10463 bp
  10% of sequence in fragments shorter than   15913 bp
  15% of sequence in fragments shorter than   20596 bp
  20% of sequence in fragments shorter than   24922 bp
  25% of sequence in fragments shorter than   29213 bp
  30% of sequence in fragments shorter than   33539 bp
  35% of sequence in fragments shorter than   37923 bp
  40% of sequence in fragments shorter than   42401 bp
  45% of sequence in fragments shorter than   47125 bp
  50% of sequence in fragments shorter than   52067 bp
  55% of sequence in fragments shorter than   57418 bp
  60% of sequence in fragments shorter than   63156 bp
  65% of sequence in fragments shorter than   69618 bp
  70% of sequence in fragments shorter than   76987 bp
  75% of sequence in fragments shorter than   85571 bp
  80% of sequence in fragments shorter than   96163 bp
  85% of sequence in fragments shorter than  110815 bp
  90% of sequence in fragments shorter than  130733 bp
  95% of sequence in fragments shorter than  176119 bp

# ClaI.NruI (ATCGAT+TCGCGA) double digest, 101 thousand fragments (86477+14872 sites), 29490 bp average fragment length
   5% of sequence in fragments shorter than   10453 bp
  10% of sequence in fragments shorter than   15852 bp
  15% of sequence in fragments shorter than   20454 bp
  20% of sequence in fragments shorter than   24725 bp
  25% of sequence in fragments shorter than   29026 bp
  30% of sequence in fragments shorter than   33311 bp
  35% of sequence in fragments shorter than   37644 bp
  40% of sequence in fragments shorter than   42039 bp
  45% of sequence in fragments shorter than   46729 bp
  50% of sequence in fragments shorter than   51632 bp
  55% of sequence in fragments shorter than   57008 bp
  60% of sequence in fragments shorter than   62742 bp
  65% of sequence in fragments shorter than   69187 bp
  70% of sequence in fragments shorter than   76416 bp
  75% of sequence in fragments shorter than   84838 bp
  80% of sequence in fragments shorter than   95582 bp
  85% of sequence in fragments shorter than  110059 bp
  90% of sequence in fragments shorter than  129972 bp
  95% of sequence in fragments shorter than  170896 bp

# PmeI.PspXI (GTTTAAAC+VCTCGAGB) double digest, 100 thousand fragments (42094+59193 sites), 29508 bp average fragment length
   5% of sequence in fragments shorter than   10220 bp
  10% of sequence in fragments shorter than   15633 bp
  15% of sequence in fragments shorter than   20262 bp
  20% of sequence in fragments shorter than   24615 bp
  25% of sequence in fragments shorter than   28894 bp
  30% of sequence in fragments shorter than   33329 bp
  35% of sequence in fragments shorter than   37845 bp
  40% of sequence in fragments shorter than   42487 bp
  45% of sequence in fragments shorter than   47589 bp
  50% of sequence in fragments shorter than   52904 bp
  55% of sequence in fragments shorter than   58499 bp
  60% of sequence in fragments shorter than   64772 bp
  65% of sequence in fragments shorter than   71592 bp
  70% of sequence in fragments shorter than   79345 bp
  75% of sequence in fragments shorter than   88777 bp
  80% of sequence in fragments shorter than   99598 bp
  85% of sequence in fragments shorter than  114105 bp
  90% of sequence in fragments shorter than  135400 bp
  95% of sequence in fragments shorter than  179401 bp

# ClaI.PvuI (ATCGAT+CGATCG) double digest, 100 thousand fragments (86477+14186 sites), 29690 bp average fragment length
   5% of sequence in fragments shorter than   10650 bp
  10% of sequence in fragments shorter than   16295 bp
  15% of sequence in fragments shorter than   21078 bp
  20% of sequence in fragments shorter than   25580 bp
  25% of sequence in fragments shorter than   30039 bp
  30% of sequence in fragments shorter than   34476 bp
  35% of sequence in fragments shorter than   38965 bp
  40% of sequence in fragments shorter than   43556 bp
  45% of sequence in fragments shorter than   48418 bp
  50% of sequence in fragments shorter than   53290 bp
  55% of sequence in fragments shorter than   58770 bp
  60% of sequence in fragments shorter than   64756 bp
  65% of sequence in fragments shorter than   71117 bp
  70% of sequence in fragments shorter than   78524 bp
  75% of sequence in fragments shorter than   87132 bp
  80% of sequence in fragments shorter than   97638 bp
  85% of sequence in fragments shorter than  112592 bp
  90% of sequence in fragments shorter than  132119 bp
  95% of sequence in fragments shorter than  174041 bp

# ClaI.FseI (ATCGAT+GGCCGGCC) double digest, 99 thousand fragments (86477+13662 sites), 29843 bp average fragment length
   5% of sequence in fragments shorter than   10575 bp
  10% of sequence in fragments shorter than   16039 bp
  15% of sequence in fragments shorter than   20766 bp
  20% of sequence in fragments shorter than   25179 bp
  25% of sequence in fragments shorter than   29581 bp
  30% of sequence in fragments shorter than   33942 bp
  35% of sequence in fragments shorter than   38414 bp
  40% of sequence in fragments shorter than   42915 bp
  45% of sequence in fragments shorter than   47652 bp
  50% of sequence in fragments shorter than   52676 bp
  55% of sequence in fragments shorter than   58253 bp
  60% of sequence in fragments shorter than   64198 bp
  65% of sequence in fragments shorter than   70857 bp
  70% of sequence in fragments shorter than   78339 bp
  75% of sequence in fragments shorter than   87193 bp
  80% of sequence in fragments shorter than   98235 bp
  85% of sequence in fragments shorter than  112995 bp
  90% of sequence in fragments shorter than  132913 bp
  95% of sequence in fragments shorter than  178460 bp

# SacII.SalI (CCGCGG+GTCGAC) double digest, 98 billion bp of sequence, 98 thousand fragments (66901+31396 sites), 30402 bp average fragment length
   5% of sequence in fragments shorter than   12785 bp
  10% of sequence in fragments shorter than   21424 bp
  15% of sequence in fragments shorter than   29470 bp
  20% of sequence in fragments shorter than   37394 bp
  25% of sequence in fragments shorter than   45317 bp
  30% of sequence in fragments shorter than   53642 bp
  35% of sequence in fragments shorter than   62419 bp
  40% of sequence in fragments shorter than   71620 bp
  45% of sequence in fragments shorter than   81731 bp
  50% of sequence in fragments shorter than   92934 bp
  55% of sequence in fragments shorter than  104662 bp
  60% of sequence in fragments shorter than  118277 bp
  65% of sequence in fragments shorter than  132885 bp
  70% of sequence in fragments shorter than  150748 bp
  75% of sequence in fragments shorter than  171300 bp
  80% of sequence in fragments shorter than  200244 bp
  85% of sequence in fragments shorter than  239105 bp
  90% of sequence in fragments shorter than  294568 bp
  95% of sequence in fragments shorter than  410477 bp

# ClaI.NotI (ATCGAT+GCGGCCGC) double digest, 95 thousand fragments (86477+9795 sites), 31044 bp average fragment length
   5% of sequence in fragments shorter than   11127 bp
  10% of sequence in fragments shorter than   16910 bp
  15% of sequence in fragments shorter than   21861 bp
  20% of sequence in fragments shorter than   26485 bp
  25% of sequence in fragments shorter than   31005 bp
  30% of sequence in fragments shorter than   35596 bp
  35% of sequence in fragments shorter than   40215 bp
  40% of sequence in fragments shorter than   44973 bp
  45% of sequence in fragments shorter than   49887 bp
  50% of sequence in fragments shorter than   55082 bp
  55% of sequence in fragments shorter than   60757 bp
  60% of sequence in fragments shorter than   66824 bp
  65% of sequence in fragments shorter than   73830 bp
  70% of sequence in fragments shorter than   81492 bp
  75% of sequence in fragments shorter than   90505 bp
  80% of sequence in fragments shorter than  101761 bp
  85% of sequence in fragments shorter than  117146 bp
  90% of sequence in fragments shorter than  138151 bp
  95% of sequence in fragments shorter than  184659 bp

# ClaI.RsrII (ATCGAT+CGGWCCG) double digest, 95 thousand fragments (86477+9764 sites), 31059 bp average fragment length
   5% of sequence in fragments shorter than   11017 bp
  10% of sequence in fragments shorter than   16717 bp
  15% of sequence in fragments shorter than   21629 bp
  20% of sequence in fragments shorter than   26260 bp
  25% of sequence in fragments shorter than   30753 bp
  30% of sequence in fragments shorter than   35332 bp
  35% of sequence in fragments shorter than   39838 bp
  40% of sequence in fragments shorter than   44476 bp
  45% of sequence in fragments shorter than   49425 bp
  50% of sequence in fragments shorter than   54700 bp
  55% of sequence in fragments shorter than   60259 bp
  60% of sequence in fragments shorter than   66416 bp
  65% of sequence in fragments shorter than   73225 bp
  70% of sequence in fragments shorter than   80742 bp
  75% of sequence in fragments shorter than   89573 bp
  80% of sequence in fragments shorter than  100882 bp
  85% of sequence in fragments shorter than  116137 bp
  90% of sequence in fragments shorter than  137126 bp
  95% of sequence in fragments shorter than  183506 bp

# AgeI.PmeI (ACCGGT+GTTTAAAC) double digest, 95 thousand fragments (53386+42094 sites), 31307 bp average fragment length
   5% of sequence in fragments shorter than   11072 bp
  10% of sequence in fragments shorter than   16672 bp
  15% of sequence in fragments shorter than   21361 bp
  20% of sequence in fragments shorter than   25875 bp
  25% of sequence in fragments shorter than   30318 bp
  30% of sequence in fragments shorter than   34683 bp
  35% of sequence in fragments shorter than   39090 bp
  40% of sequence in fragments shorter than   43718 bp
  45% of sequence in fragments shorter than   48613 bp
  50% of sequence in fragments shorter than   53807 bp
  55% of sequence in fragments shorter than   59176 bp
  60% of sequence in fragments shorter than   65141 bp
  65% of sequence in fragments shorter than   71607 bp
  70% of sequence in fragments shorter than   79099 bp
  75% of sequence in fragments shorter than   87908 bp
  80% of sequence in fragments shorter than   98771 bp
  85% of sequence in fragments shorter than  112509 bp
  90% of sequence in fragments shorter than  133272 bp
  95% of sequence in fragments shorter than  174547 bp

# AatII.MluI (GACGTC+ACGCGT) double digest, 92 thousand fragments (71089+21415 sites), 32322 bp average fragment length
   5% of sequence in fragments shorter than   10832 bp
  10% of sequence in fragments shorter than   17156 bp
  15% of sequence in fragments shorter than   22941 bp
  20% of sequence in fragments shorter than   28570 bp
  25% of sequence in fragments shorter than   34120 bp
  30% of sequence in fragments shorter than   39884 bp
  35% of sequence in fragments shorter than   46050 bp
  40% of sequence in fragments shorter than   52585 bp
  45% of sequence in fragments shorter than   59244 bp
  50% of sequence in fragments shorter than   66362 bp
  55% of sequence in fragments shorter than   74460 bp
  60% of sequence in fragments shorter than   83411 bp
  65% of sequence in fragments shorter than   92982 bp
  70% of sequence in fragments shorter than  104388 bp
  75% of sequence in fragments shorter than  117692 bp
  80% of sequence in fragments shorter than  134280 bp
  85% of sequence in fragments shorter than  157713 bp
  90% of sequence in fragments shorter than  192440 bp
  95% of sequence in fragments shorter than  254794 bp

# AscI.ClaI (GGCGCGCC+ATCGAT) double digest, 90 thousand fragments (4751+86477 sites), 32768 bp average fragment length
   5% of sequence in fragments shorter than   11620 bp
  10% of sequence in fragments shorter than   17682 bp
  15% of sequence in fragments shorter than   22830 bp
  20% of sequence in fragments shorter than   27696 bp
  25% of sequence in fragments shorter than   32403 bp
  30% of sequence in fragments shorter than   37128 bp
  35% of sequence in fragments shorter than   41908 bp
  40% of sequence in fragments shorter than   46873 bp
  45% of sequence in fragments shorter than   51979 bp
  50% of sequence in fragments shorter than   57605 bp
  55% of sequence in fragments shorter than   63332 bp
  60% of sequence in fragments shorter than   69712 bp
  65% of sequence in fragments shorter than   77039 bp
  70% of sequence in fragments shorter than   84997 bp
  75% of sequence in fragments shorter than   94431 bp
  80% of sequence in fragments shorter than  107070 bp
  85% of sequence in fragments shorter than  121713 bp
  90% of sequence in fragments shorter than  143482 bp
  95% of sequence in fragments shorter than  190927 bp

# PspXI.SalI (VCTCGAGB+GTCGAC) double digest, 90 thousand fragments (59193+31396 sites), 33004 bp average fragment length
   5% of sequence in fragments shorter than   11029 bp
  10% of sequence in fragments shorter than   17272 bp
  15% of sequence in fragments shorter than   22867 bp
  20% of sequence in fragments shorter than   28163 bp
  25% of sequence in fragments shorter than   33693 bp
  30% of sequence in fragments shorter than   39084 bp
  35% of sequence in fragments shorter than   44921 bp
  40% of sequence in fragments shorter than   51156 bp
  45% of sequence in fragments shorter than   57582 bp
  50% of sequence in fragments shorter than   64434 bp
  55% of sequence in fragments shorter than   71733 bp
  60% of sequence in fragments shorter than   79886 bp
  65% of sequence in fragments shorter than   89091 bp
  70% of sequence in fragments shorter than   99774 bp
  75% of sequence in fragments shorter than  112634 bp
  80% of sequence in fragments shorter than  129573 bp
  85% of sequence in fragments shorter than  152402 bp
  90% of sequence in fragments shorter than  186292 bp
  95% of sequence in fragments shorter than  260499 bp

# MluI.SacII (ACGCGT+CCGCGG) double digest, 88 thousand fragments (21415+66901 sites), 33839 bp average fragment length
   5% of sequence in fragments shorter than   14415 bp
  10% of sequence in fragments shorter than   25358 bp
  15% of sequence in fragments shorter than   36063 bp
  20% of sequence in fragments shorter than   46685 bp
  25% of sequence in fragments shorter than   58641 bp
  30% of sequence in fragments shorter than   70264 bp
  35% of sequence in fragments shorter than   84073 bp
  40% of sequence in fragments shorter than   98591 bp
  45% of sequence in fragments shorter than  114079 bp
  50% of sequence in fragments shorter than  131742 bp
  55% of sequence in fragments shorter than  151372 bp
  60% of sequence in fragments shorter than  173358 bp
  65% of sequence in fragments shorter than  200522 bp
  70% of sequence in fragments shorter than  232314 bp
  75% of sequence in fragments shorter than  269340 bp
  80% of sequence in fragments shorter than  320361 bp
  85% of sequence in fragments shorter than  391017 bp
  90% of sequence in fragments shorter than  490809 bp
  95% of sequence in fragments shorter than  500000 bp

# AatII.SgrAI (GACGTC+CRCCGGYG) double digest, 86 thousand fragments (71089+15270 sites), 34625 bp average fragment length
   5% of sequence in fragments shorter than   11755 bp
  10% of sequence in fragments shorter than   18476 bp
  15% of sequence in fragments shorter than   24776 bp
  20% of sequence in fragments shorter than   30792 bp
  25% of sequence in fragments shorter than   36937 bp
  30% of sequence in fragments shorter than   43250 bp
  35% of sequence in fragments shorter than   49989 bp
  40% of sequence in fragments shorter than   56648 bp
  45% of sequence in fragments shorter than   63889 bp
  50% of sequence in fragments shorter than   71734 bp
  55% of sequence in fragments shorter than   80347 bp
  60% of sequence in fragments shorter than   90327 bp
  65% of sequence in fragments shorter than  101009 bp
  70% of sequence in fragments shorter than  112911 bp
  75% of sequence in fragments shorter than  128160 bp
  80% of sequence in fragments shorter than  147401 bp
  85% of sequence in fragments shorter than  171858 bp
  90% of sequence in fragments shorter than  208876 bp
  95% of sequence in fragments shorter than  275916 bp

# AatII.NruI (GACGTC+TCGCGA) double digest, 85 thousand fragments (71089+14872 sites), 34789 bp average fragment length
   5% of sequence in fragments shorter than   11724 bp
  10% of sequence in fragments shorter than   18395 bp
  15% of sequence in fragments shorter than   24424 bp
  20% of sequence in fragments shorter than   30430 bp
  25% of sequence in fragments shorter than   36101 bp
  30% of sequence in fragments shorter than   42126 bp
  35% of sequence in fragments shorter than   48494 bp
  40% of sequence in fragments shorter than   55096 bp
  45% of sequence in fragments shorter than   61920 bp
  50% of sequence in fragments shorter than   69363 bp
  55% of sequence in fragments shorter than   77630 bp
  60% of sequence in fragments shorter than   86667 bp
  65% of sequence in fragments shorter than   96839 bp
  70% of sequence in fragments shorter than  108286 bp
  75% of sequence in fragments shorter than  122885 bp
  80% of sequence in fragments shorter than  140298 bp
  85% of sequence in fragments shorter than  164525 bp
  90% of sequence in fragments shorter than  199742 bp
  95% of sequence in fragments shorter than  268316 bp

# AatII.PvuI (GACGTC+CGATCG) double digest, 84 thousand fragments (71089+14186 sites), 35070 bp average fragment length
   5% of sequence in fragments shorter than   11840 bp
  10% of sequence in fragments shorter than   18605 bp
  15% of sequence in fragments shorter than   24773 bp
  20% of sequence in fragments shorter than   30766 bp
  25% of sequence in fragments shorter than   36675 bp
  30% of sequence in fragments shorter than   43014 bp
  35% of sequence in fragments shorter than   49652 bp
  40% of sequence in fragments shorter than   56185 bp
  45% of sequence in fragments shorter than   63404 bp
  50% of sequence in fragments shorter than   70945 bp
  55% of sequence in fragments shorter than   79317 bp
  60% of sequence in fragments shorter than   88509 bp
  65% of sequence in fragments shorter than   99001 bp
  70% of sequence in fragments shorter than  111036 bp
  75% of sequence in fragments shorter than  125211 bp
  80% of sequence in fragments shorter than  142748 bp
  85% of sequence in fragments shorter than  166273 bp
  90% of sequence in fragments shorter than  201821 bp
  95% of sequence in fragments shorter than  264419 bp

# AgeI.SalI (ACCGGT+GTCGAC) double digest, 84 thousand fragments (53386+31396 sites), 35272 bp average fragment length
   5% of sequence in fragments shorter than   12207 bp
  10% of sequence in fragments shorter than   18676 bp
  15% of sequence in fragments shorter than   24304 bp
  20% of sequence in fragments shorter than   29729 bp
  25% of sequence in fragments shorter than   35077 bp
  30% of sequence in fragments shorter than   40275 bp
  35% of sequence in fragments shorter than   45751 bp
  40% of sequence in fragments shorter than   51315 bp
  45% of sequence in fragments shorter than   57362 bp
  50% of sequence in fragments shorter than   63719 bp
  55% of sequence in fragments shorter than   70744 bp
  60% of sequence in fragments shorter than   78425 bp
  65% of sequence in fragments shorter than   86878 bp
  70% of sequence in fragments shorter than   96459 bp
  75% of sequence in fragments shorter than  107907 bp
  80% of sequence in fragments shorter than  122746 bp
  85% of sequence in fragments shorter than  141760 bp
  90% of sequence in fragments shorter than  168162 bp
  95% of sequence in fragments shorter than  223491 bp

# AatII.FseI (GACGTC+GGCCGGCC) double digest, 84 thousand fragments (71089+13662 sites), 35285 bp average fragment length
   5% of sequence in fragments shorter than   11803 bp
  10% of sequence in fragments shorter than   18702 bp
  15% of sequence in fragments shorter than   25157 bp
  20% of sequence in fragments shorter than   31379 bp
  25% of sequence in fragments shorter than   37728 bp
  30% of sequence in fragments shorter than   44361 bp
  35% of sequence in fragments shorter than   51179 bp
  40% of sequence in fragments shorter than   58199 bp
  45% of sequence in fragments shorter than   65721 bp
  50% of sequence in fragments shorter than   73943 bp
  55% of sequence in fragments shorter than   83008 bp
  60% of sequence in fragments shorter than   92961 bp
  65% of sequence in fragments shorter than  103937 bp
  70% of sequence in fragments shorter than  116968 bp
  75% of sequence in fragments shorter than  132142 bp
  80% of sequence in fragments shorter than  151873 bp
  85% of sequence in fragments shorter than  177748 bp
  90% of sequence in fragments shorter than  216346 bp
  95% of sequence in fragments shorter than  290946 bp

# SacII.SgrAI (CCGCGG+CRCCGGYG) double digest, 81 thousand fragments (66901+15270 sites), 36362 bp average fragment length
   5% of sequence in fragments shorter than   16856 bp
  10% of sequence in fragments shorter than   29988 bp
  15% of sequence in fragments shorter than   43073 bp
  20% of sequence in fragments shorter than   56870 bp
  25% of sequence in fragments shorter than   70769 bp
  30% of sequence in fragments shorter than   86375 bp
  35% of sequence in fragments shorter than  103005 bp
  40% of sequence in fragments shorter than  121661 bp
  45% of sequence in fragments shorter than  141291 bp
  50% of sequence in fragments shorter than  164513 bp
  55% of sequence in fragments shorter than  191033 bp
  60% of sequence in fragments shorter than  224601 bp
  65% of sequence in fragments shorter than  259478 bp
  70% of sequence in fragments shorter than  302620 bp
  75% of sequence in fragments shorter than  357782 bp
  80% of sequence in fragments shorter than  427348 bp
  85% of sequence in fragments shorter than  500000 bp

# NruI.SacII (TCGCGA+CCGCGG) double digest, 81 thousand fragments (14872+66901 sites), 36546 bp average fragment length
   5% of sequence in fragments shorter than   16230 bp
  10% of sequence in fragments shorter than   28220 bp
  15% of sequence in fragments shorter than   39848 bp
  20% of sequence in fragments shorter than   52259 bp
  25% of sequence in fragments shorter than   65199 bp
  30% of sequence in fragments shorter than   78667 bp
  35% of sequence in fragments shorter than   93186 bp
  40% of sequence in fragments shorter than  108775 bp
  45% of sequence in fragments shorter than  125527 bp
  50% of sequence in fragments shorter than  145130 bp
  55% of sequence in fragments shorter than  168796 bp
  60% of sequence in fragments shorter than  194570 bp
  65% of sequence in fragments shorter than  226591 bp
  70% of sequence in fragments shorter than  262191 bp
  75% of sequence in fragments shorter than  307019 bp
  80% of sequence in fragments shorter than  365275 bp
  85% of sequence in fragments shorter than  445992 bp
  90% of sequence in fragments shorter than  500000 bp

# PvuI.SacII (CGATCG+CCGCGG) double digest, 80 thousand fragments (14186+66901 sites), 36861 bp average fragment length
   5% of sequence in fragments shorter than   16267 bp
  10% of sequence in fragments shorter than   28362 bp
  15% of sequence in fragments shorter than   40405 bp
  20% of sequence in fragments shorter than   52734 bp
  25% of sequence in fragments shorter than   65676 bp
  30% of sequence in fragments shorter than   79180 bp
  35% of sequence in fragments shorter than   94557 bp
  40% of sequence in fragments shorter than  110694 bp
  45% of sequence in fragments shorter than  128374 bp
  50% of sequence in fragments shorter than  148359 bp
  55% of sequence in fragments shorter than  171265 bp
  60% of sequence in fragments shorter than  198629 bp
  65% of sequence in fragments shorter than  231041 bp
  70% of sequence in fragments shorter than  266323 bp
  75% of sequence in fragments shorter than  312284 bp
  80% of sequence in fragments shorter than  373867 bp
  85% of sequence in fragments shorter than  447644 bp
  90% of sequence in fragments shorter than  500000 bp

# AatII.NotI (GACGTC+GCGGCCGC) double digest, 80 thousand fragments (71089+9795 sites), 36977 bp average fragment length
   5% of sequence in fragments shorter than   12640 bp
  10% of sequence in fragments shorter than   19951 bp
  15% of sequence in fragments shorter than   26705 bp
  20% of sequence in fragments shorter than   33215 bp
  25% of sequence in fragments shorter than   39727 bp
  30% of sequence in fragments shorter than   46626 bp
  35% of sequence in fragments shorter than   53675 bp
  40% of sequence in fragments shorter than   60880 bp
  45% of sequence in fragments shorter than   68669 bp
  50% of sequence in fragments shorter than   76918 bp
  55% of sequence in fragments shorter than   86434 bp
  60% of sequence in fragments shorter than   96563 bp
  65% of sequence in fragments shorter than  108163 bp
  70% of sequence in fragments shorter than  121377 bp
  75% of sequence in fragments shorter than  136855 bp
  80% of sequence in fragments shorter than  157236 bp
  85% of sequence in fragments shorter than  184221 bp
  90% of sequence in fragments shorter than  224507 bp
  95% of sequence in fragments shorter than  303047 bp

# AatII.RsrII (GACGTC+CGGWCCG) double digest, 80 thousand fragments (71089+9764 sites), 36993 bp average fragment length
   5% of sequence in fragments shorter than   12433 bp
  10% of sequence in fragments shorter than   19619 bp
  15% of sequence in fragments shorter than   26215 bp
  20% of sequence in fragments shorter than   32529 bp
  25% of sequence in fragments shorter than   39024 bp
  30% of sequence in fragments shorter than   45707 bp
  35% of sequence in fragments shorter than   52725 bp
  40% of sequence in fragments shorter than   59803 bp
  45% of sequence in fragments shorter than   67482 bp
  50% of sequence in fragments shorter than   75834 bp
  55% of sequence in fragments shorter than   84842 bp
  60% of sequence in fragments shorter than   94904 bp
  65% of sequence in fragments shorter than  105930 bp
  70% of sequence in fragments shorter than  118821 bp
  75% of sequence in fragments shorter than  134224 bp
  80% of sequence in fragments shorter than  154341 bp
  85% of sequence in fragments shorter than  181238 bp
  90% of sequence in fragments shorter than  219003 bp
  95% of sequence in fragments shorter than  296193 bp

# FseI.SacII (GGCCGGCC+CCGCGG) double digest, 80 thousand fragments (13662+66901 sites), 37090 bp average fragment length
   5% of sequence in fragments shorter than   16717 bp
  10% of sequence in fragments shorter than   29913 bp
  15% of sequence in fragments shorter than   43439 bp
  20% of sequence in fragments shorter than   57683 bp
  25% of sequence in fragments shorter than   72786 bp
  30% of sequence in fragments shorter than   89439 bp
  35% of sequence in fragments shorter than  107166 bp
  40% of sequence in fragments shorter than  126539 bp
  45% of sequence in fragments shorter than  148296 bp
  50% of sequence in fragments shorter than  173869 bp
  55% of sequence in fragments shorter than  204448 bp
  60% of sequence in fragments shorter than  240073 bp
  65% of sequence in fragments shorter than  281411 bp
  70% of sequence in fragments shorter than  335092 bp
  75% of sequence in fragments shorter than  398972 bp
  80% of sequence in fragments shorter than  463680 bp
  85% of sequence in fragments shorter than  500000 bp

# MluI.PspXI (ACGCGT+VCTCGAGB) double digest, 80 thousand fragments (21415+59193 sites), 37096 bp average fragment length
   5% of sequence in fragments shorter than   12081 bp
  10% of sequence in fragments shorter than   19329 bp
  15% of sequence in fragments shorter than   25960 bp
  20% of sequence in fragments shorter than   32431 bp
  25% of sequence in fragments shorter than   38996 bp
  30% of sequence in fragments shorter than   45927 bp
  35% of sequence in fragments shorter than   53308 bp
  40% of sequence in fragments shorter than   61164 bp
  45% of sequence in fragments shorter than   69324 bp
  50% of sequence in fragments shorter than   78326 bp
  55% of sequence in fragments shorter than   88629 bp
  60% of sequence in fragments shorter than   99514 bp
  65% of sequence in fragments shorter than  111780 bp
  70% of sequence in fragments shorter than  126202 bp
  75% of sequence in fragments shorter than  143157 bp
  80% of sequence in fragments shorter than  165482 bp
  85% of sequence in fragments shorter than  196710 bp
  90% of sequence in fragments shorter than  241926 bp
  95% of sequence in fragments shorter than  338643 bp

# NotI.SacII (GCGGCCGC+CCGCGG) double digest, 76 thousand fragments (9795+66901 sites), 38964 bp average fragment length
   5% of sequence in fragments shorter than   19176 bp
  10% of sequence in fragments shorter than   34347 bp
  15% of sequence in fragments shorter than   49491 bp
  20% of sequence in fragments shorter than   65620 bp
  25% of sequence in fragments shorter than   82463 bp
  30% of sequence in fragments shorter than  100503 bp
  35% of sequence in fragments shorter than  120750 bp
  40% of sequence in fragments shorter than  142202 bp
  45% of sequence in fragments shorter than  168115 bp
  50% of sequence in fragments shorter than  198124 bp
  55% of sequence in fragments shorter than  233089 bp
  60% of sequence in fragments shorter than  273945 bp
  65% of sequence in fragments shorter than  323001 bp
  70% of sequence in fragments shorter than  385959 bp
  75% of sequence in fragments shorter than  447894 bp
  80% of sequence in fragments shorter than  500000 bp

# RsrII.SacII (CGGWCCG+CCGCGG) double digest, 76 thousand fragments (9764+66901 sites), 38989 bp average fragment length
   5% of sequence in fragments shorter than   18172 bp
  10% of sequence in fragments shorter than   32295 bp
  15% of sequence in fragments shorter than   46382 bp
  20% of sequence in fragments shorter than   61513 bp
  25% of sequence in fragments shorter than   76826 bp
  30% of sequence in fragments shorter than   93564 bp
  35% of sequence in fragments shorter than  111861 bp
  40% of sequence in fragments shorter than  131574 bp
  45% of sequence in fragments shorter than  154957 bp
  50% of sequence in fragments shorter than  178895 bp
  55% of sequence in fragments shorter than  210072 bp
  60% of sequence in fragments shorter than  245435 bp
  65% of sequence in fragments shorter than  288714 bp
  70% of sequence in fragments shorter than  339834 bp
  75% of sequence in fragments shorter than  404367 bp
  80% of sequence in fragments shorter than  477045 bp
  85% of sequence in fragments shorter than  500000 bp

# AatII.AscI (GACGTC+GGCGCGCC) double digest, 75 thousand fragments (71089+4751 sites), 39445 bp average fragment length
   5% of sequence in fragments shorter than   13329 bp
  10% of sequence in fragments shorter than   20981 bp
  15% of sequence in fragments shorter than   27851 bp
  20% of sequence in fragments shorter than   34487 bp
  25% of sequence in fragments shorter than   41242 bp
  30% of sequence in fragments shorter than   48349 bp
  35% of sequence in fragments shorter than   55244 bp
  40% of sequence in fragments shorter than   62681 bp
  45% of sequence in fragments shorter than   70635 bp
  50% of sequence in fragments shorter than   79270 bp
  55% of sequence in fragments shorter than   88879 bp
  60% of sequence in fragments shorter than   99398 bp
  65% of sequence in fragments shorter than  110878 bp
  70% of sequence in fragments shorter than  124380 bp
  75% of sequence in fragments shorter than  140871 bp
  80% of sequence in fragments shorter than  162176 bp
  85% of sequence in fragments shorter than  188615 bp
  90% of sequence in fragments shorter than  228156 bp
  95% of sequence in fragments shorter than  309408 bp

# AgeI.MluI (ACCGGT+ACGCGT) double digest, 74 thousand fragments (53386+21415 sites), 39982 bp average fragment length
   5% of sequence in fragments shorter than   13713 bp
  10% of sequence in fragments shorter than   21075 bp
  15% of sequence in fragments shorter than   27836 bp
  20% of sequence in fragments shorter than   34320 bp
  25% of sequence in fragments shorter than   40509 bp
  30% of sequence in fragments shorter than   46859 bp
  35% of sequence in fragments shorter than   53570 bp
  40% of sequence in fragments shorter than   60358 bp
  45% of sequence in fragments shorter than   67714 bp
  50% of sequence in fragments shorter than   75637 bp
  55% of sequence in fragments shorter than   84377 bp
  60% of sequence in fragments shorter than   93564 bp
  65% of sequence in fragments shorter than  104248 bp
  70% of sequence in fragments shorter than  116390 bp
  75% of sequence in fragments shorter than  130956 bp
  80% of sequence in fragments shorter than  148516 bp
  85% of sequence in fragments shorter than  172924 bp
  90% of sequence in fragments shorter than  209404 bp
  95% of sequence in fragments shorter than  292094 bp

# PspXI.SgrAI (VCTCGAGB+CRCCGGYG) double digest, 74 thousand fragments (59193+15270 sites), 40155 bp average fragment length
   5% of sequence in fragments shorter than   13210 bp
  10% of sequence in fragments shorter than   21204 bp
  15% of sequence in fragments shorter than   28314 bp
  20% of sequence in fragments shorter than   35653 bp
  25% of sequence in fragments shorter than   42993 bp
  30% of sequence in fragments shorter than   50712 bp
  35% of sequence in fragments shorter than   58746 bp
  40% of sequence in fragments shorter than   67344 bp
  45% of sequence in fragments shorter than   76378 bp
  50% of sequence in fragments shorter than   86479 bp
  55% of sequence in fragments shorter than   97268 bp
  60% of sequence in fragments shorter than  109734 bp
  65% of sequence in fragments shorter than  124046 bp
  70% of sequence in fragments shorter than  140713 bp
  75% of sequence in fragments shorter than  160970 bp
  80% of sequence in fragments shorter than  187354 bp
  85% of sequence in fragments shorter than  220174 bp
  90% of sequence in fragments shorter than  269601 bp
  95% of sequence in fragments shorter than  386334 bp

# NruI.PspXI (TCGCGA+VCTCGAGB) double digest, 73 thousand fragments (14872+59193 sites), 40382 bp average fragment length
   5% of sequence in fragments shorter than   13200 bp
  10% of sequence in fragments shorter than   20833 bp
  15% of sequence in fragments shorter than   27784 bp
  20% of sequence in fragments shorter than   34813 bp
  25% of sequence in fragments shorter than   41724 bp
  30% of sequence in fragments shorter than   48974 bp
  35% of sequence in fragments shorter than   56933 bp
  40% of sequence in fragments shorter than   64971 bp
  45% of sequence in fragments shorter than   73526 bp
  50% of sequence in fragments shorter than   82803 bp
  55% of sequence in fragments shorter than   93327 bp
  60% of sequence in fragments shorter than  104834 bp
  65% of sequence in fragments shorter than  118317 bp
  70% of sequence in fragments shorter than  133956 bp
  75% of sequence in fragments shorter than  153361 bp
  80% of sequence in fragments shorter than  176537 bp
  85% of sequence in fragments shorter than  209286 bp
  90% of sequence in fragments shorter than  256497 bp
  95% of sequence in fragments shorter than  363703 bp

# PmeI.SalI (GTTTAAAC+GTCGAC) double digest, 73 thousand fragments (42094+31396 sites), 40723 bp average fragment length
   5% of sequence in fragments shorter than   14324 bp
  10% of sequence in fragments shorter than   21718 bp
  15% of sequence in fragments shorter than   27944 bp
  20% of sequence in fragments shorter than   33848 bp
  25% of sequence in fragments shorter than   39583 bp
  30% of sequence in fragments shorter than   45412 bp
  35% of sequence in fragments shorter than   51358 bp
  40% of sequence in fragments shorter than   57525 bp
  45% of sequence in fragments shorter than   64090 bp
  50% of sequence in fragments shorter than   70806 bp
  55% of sequence in fragments shorter than   78188 bp
  60% of sequence in fragments shorter than   86263 bp
  65% of sequence in fragments shorter than   95148 bp
  70% of sequence in fragments shorter than  105210 bp
  75% of sequence in fragments shorter than  116232 bp
  80% of sequence in fragments shorter than  130841 bp
  85% of sequence in fragments shorter than  148858 bp
  90% of sequence in fragments shorter than  174887 bp
  95% of sequence in fragments shorter than  229450 bp

# PspXI.PvuI (VCTCGAGB+CGATCG) double digest, 73 thousand fragments (59193+14186 sites), 40757 bp average fragment length
   5% of sequence in fragments shorter than   13459 bp
  10% of sequence in fragments shorter than   21354 bp
  15% of sequence in fragments shorter than   28392 bp
  20% of sequence in fragments shorter than   35587 bp
  25% of sequence in fragments shorter than   42734 bp
  30% of sequence in fragments shorter than   50190 bp
  35% of sequence in fragments shorter than   58112 bp
  40% of sequence in fragments shorter than   66316 bp
  45% of sequence in fragments shorter than   74854 bp
  50% of sequence in fragments shorter than   84834 bp
  55% of sequence in fragments shorter than   95391 bp
  60% of sequence in fragments shorter than  107118 bp
  65% of sequence in fragments shorter than  120745 bp
  70% of sequence in fragments shorter than  136954 bp
  75% of sequence in fragments shorter than  155773 bp
  80% of sequence in fragments shorter than  180173 bp
  85% of sequence in fragments shorter than  213723 bp
  90% of sequence in fragments shorter than  258859 bp
  95% of sequence in fragments shorter than  363534 bp

# FseI.PspXI (GGCCGGCC+VCTCGAGB) double digest, 72 thousand fragments (13662+59193 sites), 41042 bp average fragment length
   5% of sequence in fragments shorter than   13260 bp
  10% of sequence in fragments shorter than   21359 bp
  15% of sequence in fragments shorter than   28715 bp
  20% of sequence in fragments shorter than   36391 bp
  25% of sequence in fragments shorter than   44188 bp
  30% of sequence in fragments shorter than   52203 bp
  35% of sequence in fragments shorter than   60901 bp
  40% of sequence in fragments shorter than   69625 bp
  45% of sequence in fragments shorter than   78913 bp
  50% of sequence in fragments shorter than   89801 bp
  55% of sequence in fragments shorter than  101255 bp
  60% of sequence in fragments shorter than  114558 bp
  65% of sequence in fragments shorter than  129909 bp
  70% of sequence in fragments shorter than  148157 bp
  75% of sequence in fragments shorter than  168342 bp
  80% of sequence in fragments shorter than  197112 bp
  85% of sequence in fragments shorter than  233451 bp
  90% of sequence in fragments shorter than  284420 bp
  95% of sequence in fragments shorter than  407945 bp

# AscI.SacII (GGCGCGCC+CCGCGG) double digest, 71 thousand fragments (4751+66901 sites), 41718 bp average fragment length
   5% of sequence in fragments shorter than   19841 bp
  10% of sequence in fragments shorter than   35319 bp
  15% of sequence in fragments shorter than   50774 bp
  20% of sequence in fragments shorter than   67138 bp
  25% of sequence in fragments shorter than   84589 bp
  30% of sequence in fragments shorter than  103077 bp
  35% of sequence in fragments shorter than  122985 bp
  40% of sequence in fragments shorter than  145108 bp
  45% of sequence in fragments shorter than  171217 bp
  50% of sequence in fragments shorter than  201398 bp
  55% of sequence in fragments shorter than  236298 bp
  60% of sequence in fragments shorter than  276611 bp
  65% of sequence in fragments shorter than  325342 bp
  70% of sequence in fragments shorter than  389203 bp
  75% of sequence in fragments shorter than  453691 bp
  80% of sequence in fragments shorter than  500000 bp

# NotI.PspXI (GCGGCCGC+VCTCGAGB) double digest, 68 thousand fragments (9795+59193 sites), 43348 bp average fragment length
   5% of sequence in fragments shorter than   14420 bp
  10% of sequence in fragments shorter than   23010 bp
  15% of sequence in fragments shorter than   30965 bp
  20% of sequence in fragments shorter than   38913 bp
  25% of sequence in fragments shorter than   47095 bp
  30% of sequence in fragments shorter than   55672 bp
  35% of sequence in fragments shorter than   64308 bp
  40% of sequence in fragments shorter than   73420 bp
  45% of sequence in fragments shorter than   83525 bp
  50% of sequence in fragments shorter than   94384 bp
  55% of sequence in fragments shorter than  106613 bp
  60% of sequence in fragments shorter than  120291 bp
  65% of sequence in fragments shorter than  135636 bp
  70% of sequence in fragments shorter than  154153 bp
  75% of sequence in fragments shorter than  176069 bp
  80% of sequence in fragments shorter than  206672 bp
  85% of sequence in fragments shorter than  244422 bp
  90% of sequence in fragments shorter than  298107 bp
  95% of sequence in fragments shorter than  442652 bp

# PspXI.RsrII (VCTCGAGB+CGGWCCG) double digest, 68 thousand fragments (59193+9764 sites), 43380 bp average fragment length
   5% of sequence in fragments shorter than   14172 bp
  10% of sequence in fragments shorter than   22558 bp
  15% of sequence in fragments shorter than   30295 bp
  20% of sequence in fragments shorter than   38095 bp
  25% of sequence in fragments shorter than   45896 bp
  30% of sequence in fragments shorter than   54377 bp
  35% of sequence in fragments shorter than   62753 bp
  40% of sequence in fragments shorter than   71677 bp
  45% of sequence in fragments shorter than   81516 bp
  50% of sequence in fragments shorter than   92084 bp
  55% of sequence in fragments shorter than  103537 bp
  60% of sequence in fragments shorter than  116870 bp
  65% of sequence in fragments shorter than  131994 bp
  70% of sequence in fragments shorter than  150136 bp
  75% of sequence in fragments shorter than  170850 bp
  80% of sequence in fragments shorter than  200820 bp
  85% of sequence in fragments shorter than  236680 bp
  90% of sequence in fragments shorter than  291047 bp
  95% of sequence in fragments shorter than  420603 bp

# AgeI.SgrAI (ACCGGT+CRCCGGYG) double digest, 68 thousand fragments (53386+15270 sites), 43561 bp average fragment length
   5% of sequence in fragments shorter than   16604 bp
  10% of sequence in fragments shorter than   25500 bp
  15% of sequence in fragments shorter than   33517 bp
  20% of sequence in fragments shorter than   40939 bp
  25% of sequence in fragments shorter than   48377 bp
  30% of sequence in fragments shorter than   55838 bp
  35% of sequence in fragments shorter than   63451 bp
  40% of sequence in fragments shorter than   71614 bp
  45% of sequence in fragments shorter than   80334 bp
  50% of sequence in fragments shorter than   89624 bp
  55% of sequence in fragments shorter than   99404 bp
  60% of sequence in fragments shorter than  110280 bp
  65% of sequence in fragments shorter than  122589 bp
  70% of sequence in fragments shorter than  136209 bp
  75% of sequence in fragments shorter than  152858 bp
  80% of sequence in fragments shorter than  173385 bp
  85% of sequence in fragments shorter than  202652 bp
  90% of sequence in fragments shorter than  248077 bp
  95% of sequence in fragments shorter than  341401 bp

# AgeI.NruI (ACCGGT+TCGCGA) double digest, 67 thousand fragments (53386+14872 sites), 43827 bp average fragment length
   5% of sequence in fragments shorter than   15225 bp
  10% of sequence in fragments shorter than   23054 bp
  15% of sequence in fragments shorter than   30320 bp
  20% of sequence in fragments shorter than   37099 bp
  25% of sequence in fragments shorter than   43597 bp
  30% of sequence in fragments shorter than   50289 bp
  35% of sequence in fragments shorter than   57302 bp
  40% of sequence in fragments shorter than   64515 bp
  45% of sequence in fragments shorter than   72278 bp
  50% of sequence in fragments shorter than   80763 bp
  55% of sequence in fragments shorter than   89684 bp
  60% of sequence in fragments shorter than   99432 bp
  65% of sequence in fragments shorter than  110449 bp
  70% of sequence in fragments shorter than  123430 bp
  75% of sequence in fragments shorter than  138588 bp
  80% of sequence in fragments shorter than  157744 bp
  85% of sequence in fragments shorter than  181666 bp
  90% of sequence in fragments shorter than  219784 bp
  95% of sequence in fragments shorter than  298909 bp

# AgeI.PvuI (ACCGGT+CGATCG) double digest, 67 thousand fragments (53386+14186 sites), 44275 bp average fragment length
   5% of sequence in fragments shorter than   15427 bp
  10% of sequence in fragments shorter than   23597 bp
  15% of sequence in fragments shorter than   31196 bp
  20% of sequence in fragments shorter than   38171 bp
  25% of sequence in fragments shorter than   45085 bp
  30% of sequence in fragments shorter than   51868 bp
  35% of sequence in fragments shorter than   58968 bp
  40% of sequence in fragments shorter than   66373 bp
  45% of sequence in fragments shorter than   74313 bp
  50% of sequence in fragments shorter than   82866 bp
  55% of sequence in fragments shorter than   91958 bp
  60% of sequence in fragments shorter than  101734 bp
  65% of sequence in fragments shorter than  112881 bp
  70% of sequence in fragments shorter than  125654 bp
  75% of sequence in fragments shorter than  140539 bp
  80% of sequence in fragments shorter than  159465 bp
  85% of sequence in fragments shorter than  183007 bp
  90% of sequence in fragments shorter than  222069 bp
  95% of sequence in fragments shorter than  301168 bp

# AgeI.FseI (ACCGGT+GGCCGGCC) double digest, 66 thousand fragments (53386+13662 sites), 44610 bp average fragment length
   5% of sequence in fragments shorter than   15342 bp
  10% of sequence in fragments shorter than   23624 bp
  15% of sequence in fragments shorter than   31331 bp
  20% of sequence in fragments shorter than   38545 bp
  25% of sequence in fragments shorter than   45815 bp
  30% of sequence in fragments shorter than   53025 bp
  35% of sequence in fragments shorter than   60517 bp
  40% of sequence in fragments shorter than   68726 bp
  45% of sequence in fragments shorter than   77292 bp
  50% of sequence in fragments shorter than   86422 bp
  55% of sequence in fragments shorter than   95947 bp
  60% of sequence in fragments shorter than  106716 bp
  65% of sequence in fragments shorter than  118900 bp
  70% of sequence in fragments shorter than  133315 bp
  75% of sequence in fragments shorter than  149228 bp
  80% of sequence in fragments shorter than  169082 bp
  85% of sequence in fragments shorter than  196740 bp
  90% of sequence in fragments shorter than  237429 bp
  95% of sequence in fragments shorter than  324298 bp

# AscI.PspXI (GGCGCGCC+VCTCGAGB) double digest, 63 thousand fragments (4751+59193 sites), 46786 bp average fragment length
   5% of sequence in fragments shorter than   15336 bp
  10% of sequence in fragments shorter than   24225 bp
  15% of sequence in fragments shorter than   32566 bp
  20% of sequence in fragments shorter than   40701 bp
  25% of sequence in fragments shorter than   49230 bp
  30% of sequence in fragments shorter than   57915 bp
  35% of sequence in fragments shorter than   66859 bp
  40% of sequence in fragments shorter than   76207 bp
  45% of sequence in fragments shorter than   86780 bp
  50% of sequence in fragments shorter than   97601 bp
  55% of sequence in fragments shorter than  109920 bp
  60% of sequence in fragments shorter than  124366 bp
  65% of sequence in fragments shorter than  139835 bp
  70% of sequence in fragments shorter than  159133 bp
  75% of sequence in fragments shorter than  181957 bp
  80% of sequence in fragments shorter than  211704 bp
  85% of sequence in fragments shorter than  250124 bp
  90% of sequence in fragments shorter than  303437 bp
  95% of sequence in fragments shorter than  448392 bp

# MluI.PmeI (ACGCGT+GTTTAAAC) double digest, 63 thousand fragments (21415+42094 sites), 47138 bp average fragment length
   5% of sequence in fragments shorter than   16698 bp
  10% of sequence in fragments shorter than   25168 bp
  15% of sequence in fragments shorter than   32640 bp
  20% of sequence in fragments shorter than   39590 bp
  25% of sequence in fragments shorter than   46745 bp
  30% of sequence in fragments shorter than   53736 bp
  35% of sequence in fragments shorter than   60817 bp
  40% of sequence in fragments shorter than   68133 bp
  45% of sequence in fragments shorter than   75823 bp
  50% of sequence in fragments shorter than   83815 bp
  55% of sequence in fragments shorter than   92754 bp
  60% of sequence in fragments shorter than  102414 bp
  65% of sequence in fragments shorter than  112507 bp
  70% of sequence in fragments shorter than  124956 bp
  75% of sequence in fragments shorter than  138958 bp
  80% of sequence in fragments shorter than  154899 bp
  85% of sequence in fragments shorter than  178730 bp
  90% of sequence in fragments shorter than  213266 bp
  95% of sequence in fragments shorter than  287402 bp

# AgeI.NotI (ACCGGT+GCGGCCGC) double digest, 62 thousand fragments (53386+9795 sites), 47350 bp average fragment length
   5% of sequence in fragments shorter than   16688 bp
  10% of sequence in fragments shorter than   25726 bp
  15% of sequence in fragments shorter than   33837 bp
  20% of sequence in fragments shorter than   41377 bp
  25% of sequence in fragments shorter than   49012 bp
  30% of sequence in fragments shorter than   56672 bp
  35% of sequence in fragments shorter than   64493 bp
  40% of sequence in fragments shorter than   72678 bp
  45% of sequence in fragments shorter than   81533 bp
  50% of sequence in fragments shorter than   90861 bp
  55% of sequence in fragments shorter than  100808 bp
  60% of sequence in fragments shorter than  111991 bp
  65% of sequence in fragments shorter than  125132 bp
  70% of sequence in fragments shorter than  139414 bp
  75% of sequence in fragments shorter than  156395 bp
  80% of sequence in fragments shorter than  177485 bp
  85% of sequence in fragments shorter than  206552 bp
  90% of sequence in fragments shorter than  251887 bp
  95% of sequence in fragments shorter than  350131 bp

# AgeI.RsrII (ACCGGT+CGGWCCG) double digest, 62 thousand fragments (53386+9764 sites), 47387 bp average fragment length
   5% of sequence in fragments shorter than   16520 bp
  10% of sequence in fragments shorter than   25396 bp
  15% of sequence in fragments shorter than   33324 bp
  20% of sequence in fragments shorter than   40671 bp
  25% of sequence in fragments shorter than   48202 bp
  30% of sequence in fragments shorter than   55827 bp
  35% of sequence in fragments shorter than   63419 bp
  40% of sequence in fragments shorter than   71589 bp
  45% of sequence in fragments shorter than   80381 bp
  50% of sequence in fragments shorter than   89615 bp
  55% of sequence in fragments shorter than   99501 bp
  60% of sequence in fragments shorter than  110447 bp
  65% of sequence in fragments shorter than  122997 bp
  70% of sequence in fragments shorter than  137664 bp
  75% of sequence in fragments shorter than  154814 bp
  80% of sequence in fragments shorter than  176077 bp
  85% of sequence in fragments shorter than  203577 bp
  90% of sequence in fragments shorter than  248699 bp
  95% of sequence in fragments shorter than  343443 bp

# AgeI.AscI (ACCGGT+GGCGCGCC) double digest, 57 thousand fragments (53386+4751 sites), 51477 bp average fragment length
   5% of sequence in fragments shorter than   17960 bp
  10% of sequence in fragments shorter than   27449 bp
  15% of sequence in fragments shorter than   35883 bp
  20% of sequence in fragments shorter than   43721 bp
  25% of sequence in fragments shorter than   51476 bp
  30% of sequence in fragments shorter than   59366 bp
  35% of sequence in fragments shorter than   67439 bp
  40% of sequence in fragments shorter than   76001 bp
  45% of sequence in fragments shorter than   85181 bp
  50% of sequence in fragments shorter than   94690 bp
  55% of sequence in fragments shorter than  104646 bp
  60% of sequence in fragments shorter than  116170 bp
  65% of sequence in fragments shorter than  129287 bp
  70% of sequence in fragments shorter than  143977 bp
  75% of sequence in fragments shorter than  160977 bp
  80% of sequence in fragments shorter than  182307 bp
  85% of sequence in fragments shorter than  211250 bp
  90% of sequence in fragments shorter than  257880 bp
  95% of sequence in fragments shorter than  355127 bp

# PmeI.SgrAI (GTTTAAAC+CRCCGGYG) double digest, 57 thousand fragments (42094+15270 sites), 52195 bp average fragment length
   5% of sequence in fragments shorter than   18829 bp
  10% of sequence in fragments shorter than   28265 bp
  15% of sequence in fragments shorter than   36543 bp
  20% of sequence in fragments shorter than   44378 bp
  25% of sequence in fragments shorter than   52145 bp
  30% of sequence in fragments shorter than   59936 bp
  35% of sequence in fragments shorter than   67594 bp
  40% of sequence in fragments shorter than   75586 bp
  45% of sequence in fragments shorter than   83735 bp
  50% of sequence in fragments shorter than   92721 bp
  55% of sequence in fragments shorter than  102269 bp
  60% of sequence in fragments shorter than  112667 bp
  65% of sequence in fragments shorter than  124635 bp
  70% of sequence in fragments shorter than  138434 bp
  75% of sequence in fragments shorter than  153472 bp
  80% of sequence in fragments shorter than  173324 bp
  85% of sequence in fragments shorter than  198332 bp
  90% of sequence in fragments shorter than  236959 bp
  95% of sequence in fragments shorter than  310981 bp

# NruI.PmeI (TCGCGA+GTTTAAAC) double digest, 56 thousand fragments (14872+42094 sites), 52575 bp average fragment length
   5% of sequence in fragments shorter than   18623 bp
  10% of sequence in fragments shorter than   28016 bp
  15% of sequence in fragments shorter than   36456 bp
  20% of sequence in fragments shorter than   44220 bp
  25% of sequence in fragments shorter than   51859 bp
  30% of sequence in fragments shorter than   59359 bp
  35% of sequence in fragments shorter than   67063 bp
  40% of sequence in fragments shorter than   74919 bp
  45% of sequence in fragments shorter than   83212 bp
  50% of sequence in fragments shorter than   91839 bp
  55% of sequence in fragments shorter than  101350 bp
  60% of sequence in fragments shorter than  111653 bp
  65% of sequence in fragments shorter than  123482 bp
  70% of sequence in fragments shorter than  136635 bp
  75% of sequence in fragments shorter than  152021 bp
  80% of sequence in fragments shorter than  169874 bp
  85% of sequence in fragments shorter than  193312 bp
  90% of sequence in fragments shorter than  230324 bp
  95% of sequence in fragments shorter than  304083 bp

# PmeI.PvuI (GTTTAAAC+CGATCG) double digest, 55 thousand fragments (42094+14186 sites), 53220 bp average fragment length
   5% of sequence in fragments shorter than   19149 bp
  10% of sequence in fragments shorter than   28795 bp
  15% of sequence in fragments shorter than   37147 bp
  20% of sequence in fragments shorter than   45466 bp
  25% of sequence in fragments shorter than   53265 bp
  30% of sequence in fragments shorter than   61285 bp
  35% of sequence in fragments shorter than   68849 bp
  40% of sequence in fragments shorter than   77089 bp
  45% of sequence in fragments shorter than   85869 bp
  50% of sequence in fragments shorter than   94869 bp
  55% of sequence in fragments shorter than  104364 bp
  60% of sequence in fragments shorter than  114986 bp
  65% of sequence in fragments shorter than  126857 bp
  70% of sequence in fragments shorter than  140281 bp
  75% of sequence in fragments shorter than  155314 bp
  80% of sequence in fragments shorter than  175236 bp
  85% of sequence in fragments shorter than  198852 bp
  90% of sequence in fragments shorter than  236860 bp
  95% of sequence in fragments shorter than  317600 bp

# FseI.PmeI (GGCCGGCC+GTTTAAAC) double digest, 55 thousand fragments (13662+42094 sites), 53707 bp average fragment length
   5% of sequence in fragments shorter than   19132 bp
  10% of sequence in fragments shorter than   28810 bp
  15% of sequence in fragments shorter than   37489 bp
  20% of sequence in fragments shorter than   45616 bp
  25% of sequence in fragments shorter than   53507 bp
  30% of sequence in fragments shorter than   61753 bp
  35% of sequence in fragments shorter than   69738 bp
  40% of sequence in fragments shorter than   77712 bp
  45% of sequence in fragments shorter than   86869 bp
  50% of sequence in fragments shorter than   96013 bp
  55% of sequence in fragments shorter than  106055 bp
  60% of sequence in fragments shorter than  116290 bp
  65% of sequence in fragments shorter than  128709 bp
  70% of sequence in fragments shorter than  142510 bp
  75% of sequence in fragments shorter than  157758 bp
  80% of sequence in fragments shorter than  178680 bp
  85% of sequence in fragments shorter than  205135 bp
  90% of sequence in fragments shorter than  244970 bp
  95% of sequence in fragments shorter than  326243 bp

# MluI.SalI (ACGCGT+GTCGAC) double digest, 52 thousand fragments (21415+31396 sites), 56727 bp average fragment length
   5% of sequence in fragments shorter than   19416 bp
  10% of sequence in fragments shorter than   30357 bp
  15% of sequence in fragments shorter than   39907 bp
  20% of sequence in fragments shorter than   49208 bp
  25% of sequence in fragments shorter than   58607 bp
  30% of sequence in fragments shorter than   68336 bp
  35% of sequence in fragments shorter than   78236 bp
  40% of sequence in fragments shorter than   88741 bp
  45% of sequence in fragments shorter than   99234 bp
  50% of sequence in fragments shorter than  110915 bp
  55% of sequence in fragments shorter than  124114 bp
  60% of sequence in fragments shorter than  138428 bp
  65% of sequence in fragments shorter than  154578 bp
  70% of sequence in fragments shorter than  174117 bp
  75% of sequence in fragments shorter than  196020 bp
  80% of sequence in fragments shorter than  223902 bp
  85% of sequence in fragments shorter than  258742 bp
  90% of sequence in fragments shorter than  313042 bp
  95% of sequence in fragments shorter than  426024 bp

# NotI.PmeI (GCGGCCGC+GTTTAAAC) double digest, 51 thousand fragments (9795+42094 sites), 57722 bp average fragment length
   5% of sequence in fragments shorter than   20887 bp
  10% of sequence in fragments shorter than   31464 bp
  15% of sequence in fragments shorter than   40603 bp
  20% of sequence in fragments shorter than   49628 bp
  25% of sequence in fragments shorter than   58382 bp
  30% of sequence in fragments shorter than   66925 bp
  35% of sequence in fragments shorter than   75476 bp
  40% of sequence in fragments shorter than   84492 bp
  45% of sequence in fragments shorter than   94134 bp
  50% of sequence in fragments shorter than  104025 bp
  55% of sequence in fragments shorter than  114727 bp
  60% of sequence in fragments shorter than  126396 bp
  65% of sequence in fragments shorter than  139499 bp
  70% of sequence in fragments shorter than  154267 bp
  75% of sequence in fragments shorter than  172465 bp
  80% of sequence in fragments shorter than  193334 bp
  85% of sequence in fragments shorter than  222300 bp
  90% of sequence in fragments shorter than  264061 bp
  95% of sequence in fragments shorter than  354140 bp

# PmeI.RsrII (GTTTAAAC+CGGWCCG) double digest, 51 thousand fragments (42094+9764 sites), 57776 bp average fragment length
   5% of sequence in fragments shorter than   20551 bp
  10% of sequence in fragments shorter than   30904 bp
  15% of sequence in fragments shorter than   39911 bp
  20% of sequence in fragments shorter than   48715 bp
  25% of sequence in fragments shorter than   57316 bp
  30% of sequence in fragments shorter than   65696 bp
  35% of sequence in fragments shorter than   73905 bp
  40% of sequence in fragments shorter than   82772 bp
  45% of sequence in fragments shorter than   91813 bp
  50% of sequence in fragments shorter than  101549 bp
  55% of sequence in fragments shorter than  111492 bp
  60% of sequence in fragments shorter than  123234 bp
  65% of sequence in fragments shorter than  136146 bp
  70% of sequence in fragments shorter than  149568 bp
  75% of sequence in fragments shorter than  167003 bp
  80% of sequence in fragments shorter than  187886 bp
  85% of sequence in fragments shorter than  215850 bp
  90% of sequence in fragments shorter than  257781 bp
  95% of sequence in fragments shorter than  347910 bp

# AscI.PmeI (GGCGCGCC+GTTTAAAC) double digest, 46 thousand fragments (4751+42094 sites), 63982 bp average fragment length
   5% of sequence in fragments shorter than   22607 bp
  10% of sequence in fragments shorter than   34187 bp
  15% of sequence in fragments shorter than   44181 bp
  20% of sequence in fragments shorter than   53769 bp
  25% of sequence in fragments shorter than   63242 bp
  30% of sequence in fragments shorter than   72111 bp
  35% of sequence in fragments shorter than   81802 bp
  40% of sequence in fragments shorter than   91562 bp
  45% of sequence in fragments shorter than  101841 bp
  50% of sequence in fragments shorter than  112319 bp
  55% of sequence in fragments shorter than  124121 bp
  60% of sequence in fragments shorter than  137175 bp
  65% of sequence in fragments shorter than  150932 bp
  70% of sequence in fragments shorter than  167511 bp
  75% of sequence in fragments shorter than  186419 bp
  80% of sequence in fragments shorter than  210291 bp
  85% of sequence in fragments shorter than  242379 bp
  90% of sequence in fragments shorter than  290296 bp
  95% of sequence in fragments shorter than  394689 bp

# SalI.SgrAI (GTCGAC+CRCCGGYG) double digest, 46 thousand fragments (31396+15270 sites), 64221 bp average fragment length
   5% of sequence in fragments shorter than   22473 bp
  10% of sequence in fragments shorter than   34994 bp
  15% of sequence in fragments shorter than   46238 bp
  20% of sequence in fragments shorter than   56756 bp
  25% of sequence in fragments shorter than   67533 bp
  30% of sequence in fragments shorter than   78506 bp
  35% of sequence in fragments shorter than   90015 bp
  40% of sequence in fragments shorter than  101951 bp
  45% of sequence in fragments shorter than  114174 bp
  50% of sequence in fragments shorter than  128131 bp
  55% of sequence in fragments shorter than  142738 bp
  60% of sequence in fragments shorter than  158443 bp
  65% of sequence in fragments shorter than  178156 bp
  70% of sequence in fragments shorter than  199158 bp
  75% of sequence in fragments shorter than  225510 bp
  80% of sequence in fragments shorter than  253847 bp
  85% of sequence in fragments shorter than  292867 bp
  90% of sequence in fragments shorter than  361397 bp
  95% of sequence in fragments shorter than  498906 bp

# NruI.SalI (TCGCGA+GTCGAC) double digest, 45 thousand fragments (14872+31396 sites), 64792 bp average fragment length
   5% of sequence in fragments shorter than   22286 bp
  10% of sequence in fragments shorter than   34357 bp
  15% of sequence in fragments shorter than   45002 bp
  20% of sequence in fragments shorter than   55141 bp
  25% of sequence in fragments shorter than   65275 bp
  30% of sequence in fragments shorter than   75688 bp
  35% of sequence in fragments shorter than   86523 bp
  40% of sequence in fragments shorter than   97590 bp
  45% of sequence in fragments shorter than  109447 bp
  50% of sequence in fragments shorter than  122258 bp
  55% of sequence in fragments shorter than  136543 bp
  60% of sequence in fragments shorter than  152755 bp
  65% of sequence in fragments shorter than  169580 bp
  70% of sequence in fragments shorter than  190484 bp
  75% of sequence in fragments shorter than  213853 bp
  80% of sequence in fragments shorter than  243225 bp
  85% of sequence in fragments shorter than  280208 bp
  90% of sequence in fragments shorter than  334720 bp
  95% of sequence in fragments shorter than  473101 bp

# PvuI.SalI (CGATCG+GTCGAC) double digest, 45 thousand fragments (14186+31396 sites), 65766 bp average fragment length
   5% of sequence in fragments shorter than   22850 bp
  10% of sequence in fragments shorter than   35701 bp
  15% of sequence in fragments shorter than   46860 bp
  20% of sequence in fragments shorter than   57619 bp
  25% of sequence in fragments shorter than   68127 bp
  30% of sequence in fragments shorter than   78612 bp
  35% of sequence in fragments shorter than   89389 bp
  40% of sequence in fragments shorter than  100662 bp
  45% of sequence in fragments shorter than  112555 bp
  50% of sequence in fragments shorter than  125734 bp
  55% of sequence in fragments shorter than  140693 bp
  60% of sequence in fragments shorter than  156579 bp
  65% of sequence in fragments shorter than  174157 bp
  70% of sequence in fragments shorter than  195576 bp
  75% of sequence in fragments shorter than  219831 bp
  80% of sequence in fragments shorter than  248872 bp
  85% of sequence in fragments shorter than  288019 bp
  90% of sequence in fragments shorter than  350090 bp
  95% of sequence in fragments shorter than  493696 bp

# FseI.SalI (GGCCGGCC+GTCGAC) double digest, 44 thousand fragments (13662+31396 sites), 66520 bp average fragment length
   5% of sequence in fragments shorter than   22481 bp
  10% of sequence in fragments shorter than   35730 bp
  15% of sequence in fragments shorter than   47571 bp
  20% of sequence in fragments shorter than   58836 bp
  25% of sequence in fragments shorter than   70465 bp
  30% of sequence in fragments shorter than   82057 bp
  35% of sequence in fragments shorter than   93971 bp
  40% of sequence in fragments shorter than  106881 bp
  45% of sequence in fragments shorter than  119939 bp
  50% of sequence in fragments shorter than  134796 bp
  55% of sequence in fragments shorter than  151553 bp
  60% of sequence in fragments shorter than  168381 bp
  65% of sequence in fragments shorter than  189389 bp
  70% of sequence in fragments shorter than  212647 bp
  75% of sequence in fragments shorter than  240256 bp
  80% of sequence in fragments shorter than  271554 bp
  85% of sequence in fragments shorter than  316612 bp
  90% of sequence in fragments shorter than  389156 bp
  95% of sequence in fragments shorter than  500000 bp

# NotI.SalI (GCGGCCGC+GTCGAC) double digest, 40 thousand fragments (9795+31396 sites), 72800 bp average fragment length
   5% of sequence in fragments shorter than   25597 bp
  10% of sequence in fragments shorter than   40367 bp
  15% of sequence in fragments shorter than   53269 bp
  20% of sequence in fragments shorter than   65321 bp
  25% of sequence in fragments shorter than   77636 bp
  30% of sequence in fragments shorter than   89965 bp
  35% of sequence in fragments shorter than  102601 bp
  40% of sequence in fragments shorter than  115737 bp
  45% of sequence in fragments shorter than  130128 bp
  50% of sequence in fragments shorter than  145970 bp
  55% of sequence in fragments shorter than  162028 bp
  60% of sequence in fragments shorter than  181174 bp
  65% of sequence in fragments shorter than  203918 bp
  70% of sequence in fragments shorter than  228428 bp
  75% of sequence in fragments shorter than  255276 bp
  80% of sequence in fragments shorter than  290964 bp
  85% of sequence in fragments shorter than  341469 bp
  90% of sequence in fragments shorter than  415704 bp
  95% of sequence in fragments shorter than  500000 bp

# RsrII.SalI (CGGWCCG+GTCGAC) double digest, 40 thousand fragments (9764+31396 sites), 72882 bp average fragment length
   5% of sequence in fragments shorter than   25160 bp
  10% of sequence in fragments shorter than   39111 bp
  15% of sequence in fragments shorter than   51739 bp
  20% of sequence in fragments shorter than   63740 bp
  25% of sequence in fragments shorter than   75458 bp
  30% of sequence in fragments shorter than   87416 bp
  35% of sequence in fragments shorter than   99386 bp
  40% of sequence in fragments shorter than  112444 bp
  45% of sequence in fragments shorter than  126193 bp
  50% of sequence in fragments shorter than  141980 bp
  55% of sequence in fragments shorter than  157208 bp
  60% of sequence in fragments shorter than  175979 bp
  65% of sequence in fragments shorter than  196344 bp
  70% of sequence in fragments shorter than  221133 bp
  75% of sequence in fragments shorter than  248140 bp
  80% of sequence in fragments shorter than  280769 bp
  85% of sequence in fragments shorter than  327225 bp
  90% of sequence in fragments shorter than  404616 bp
  95% of sequence in fragments shorter than  500000 bp

# MluI.SgrAI (ACGCGT+CRCCGGYG) double digest, 36 thousand fragments (21415+15270 sites), 81744 bp average fragment length
   5% of sequence in fragments shorter than   27716 bp
  10% of sequence in fragments shorter than   45347 bp
  15% of sequence in fragments shorter than   61469 bp
  20% of sequence in fragments shorter than   78929 bp
  25% of sequence in fragments shorter than   96340 bp
  30% of sequence in fragments shorter than  114126 bp
  35% of sequence in fragments shorter than  133667 bp
  40% of sequence in fragments shorter than  154162 bp
  45% of sequence in fragments shorter than  177591 bp
  50% of sequence in fragments shorter than  202920 bp
  55% of sequence in fragments shorter than  230868 bp
  60% of sequence in fragments shorter than  260580 bp
  65% of sequence in fragments shorter than  295635 bp
  70% of sequence in fragments shorter than  335611 bp
  75% of sequence in fragments shorter than  387242 bp
  80% of sequence in fragments shorter than  454566 bp
  85% of sequence in fragments shorter than  500000 bp

# MluI.NruI (ACGCGT+TCGCGA) double digest, 36 thousand fragments (21415+14872 sites), 82681 bp average fragment length
   5% of sequence in fragments shorter than   27433 bp
  10% of sequence in fragments shorter than   44016 bp
  15% of sequence in fragments shorter than   59858 bp
  20% of sequence in fragments shorter than   75441 bp
  25% of sequence in fragments shorter than   91291 bp
  30% of sequence in fragments shorter than  107976 bp
  35% of sequence in fragments shorter than  125408 bp
  40% of sequence in fragments shorter than  143474 bp
  45% of sequence in fragments shorter than  163292 bp
  50% of sequence in fragments shorter than  186962 bp
  55% of sequence in fragments shorter than  211568 bp
  60% of sequence in fragments shorter than  238546 bp
  65% of sequence in fragments shorter than  270845 bp
  70% of sequence in fragments shorter than  308698 bp
  75% of sequence in fragments shorter than  349596 bp
  80% of sequence in fragments shorter than  410573 bp
  85% of sequence in fragments shorter than  481046 bp
  90% of sequence in fragments shorter than  500000 bp

# AscI.SalI (GGCGCGCC+GTCGAC) double digest, 35 thousand fragments (4751+31396 sites), 83029 bp average fragment length
   5% of sequence in fragments shorter than   29012 bp
  10% of sequence in fragments shorter than   44840 bp
  15% of sequence in fragments shorter than   58664 bp
  20% of sequence in fragments shorter than   71562 bp
  25% of sequence in fragments shorter than   84502 bp
  30% of sequence in fragments shorter than   97136 bp
  35% of sequence in fragments shorter than  110331 bp
  40% of sequence in fragments shorter than  124041 bp
  45% of sequence in fragments shorter than  140004 bp
  50% of sequence in fragments shorter than  155922 bp
  55% of sequence in fragments shorter than  174337 bp
  60% of sequence in fragments shorter than  194709 bp
  65% of sequence in fragments shorter than  217041 bp
  70% of sequence in fragments shorter than  242903 bp
  75% of sequence in fragments shorter than  270201 bp
  80% of sequence in fragments shorter than  305611 bp
  85% of sequence in fragments shorter than  360935 bp
  90% of sequence in fragments shorter than  433418 bp
  95% of sequence in fragments shorter than  500000 bp

# MluI.PvuI (ACGCGT+CGATCG) double digest, 35 thousand fragments (21415+14186 sites), 84290 bp average fragment length
   5% of sequence in fragments shorter than   28366 bp
  10% of sequence in fragments shorter than   45652 bp
  15% of sequence in fragments shorter than   62170 bp
  20% of sequence in fragments shorter than   79024 bp
  25% of sequence in fragments shorter than   96151 bp
  30% of sequence in fragments shorter than  113200 bp
  35% of sequence in fragments shorter than  131561 bp
  40% of sequence in fragments shorter than  150185 bp
  45% of sequence in fragments shorter than  171225 bp
  50% of sequence in fragments shorter than  196335 bp
  55% of sequence in fragments shorter than  223942 bp
  60% of sequence in fragments shorter than  250110 bp
  65% of sequence in fragments shorter than  283374 bp
  70% of sequence in fragments shorter than  319016 bp
  75% of sequence in fragments shorter than  365658 bp
  80% of sequence in fragments shorter than  425298 bp
  85% of sequence in fragments shorter than  500000 bp

# FseI.MluI (GGCCGGCC+ACGCGT) double digest, 34 thousand fragments (13662+21415 sites), 85484 bp average fragment length
   5% of sequence in fragments shorter than   27828 bp
  10% of sequence in fragments shorter than   46597 bp
  15% of sequence in fragments shorter than   64623 bp
  20% of sequence in fragments shorter than   84316 bp
  25% of sequence in fragments shorter than  103825 bp
  30% of sequence in fragments shorter than  123273 bp
  35% of sequence in fragments shorter than  144728 bp
  40% of sequence in fragments shorter than  168272 bp
  45% of sequence in fragments shorter than  193978 bp
  50% of sequence in fragments shorter than  221974 bp
  55% of sequence in fragments shorter than  256136 bp
  60% of sequence in fragments shorter than  290397 bp
  65% of sequence in fragments shorter than  330670 bp
  70% of sequence in fragments shorter than  384814 bp
  75% of sequence in fragments shorter than  438835 bp
  80% of sequence in fragments shorter than  500000 bp

# MluI.NotI (ACGCGT+GCGGCCGC) double digest, 30 thousand fragments (21415+9795 sites), 96169 bp average fragment length
   5% of sequence in fragments shorter than   33027 bp
  10% of sequence in fragments shorter than   54524 bp
  15% of sequence in fragments shorter than   75936 bp
  20% of sequence in fragments shorter than   97496 bp
  25% of sequence in fragments shorter than  119498 bp
  30% of sequence in fragments shorter than  142141 bp
  35% of sequence in fragments shorter than  166160 bp
  40% of sequence in fragments shorter than  192500 bp
  45% of sequence in fragments shorter than  220390 bp
  50% of sequence in fragments shorter than  253115 bp
  55% of sequence in fragments shorter than  289539 bp
  60% of sequence in fragments shorter than  330233 bp
  65% of sequence in fragments shorter than  380023 bp
  70% of sequence in fragments shorter than  440982 bp
  75% of sequence in fragments shorter than  500000 bp

# MluI.RsrII (ACGCGT+CGGWCCG) double digest, 30 thousand fragments (21415+9764 sites), 96302 bp average fragment length
   5% of sequence in fragments shorter than   31882 bp
  10% of sequence in fragments shorter than   51810 bp
  15% of sequence in fragments shorter than   71681 bp
  20% of sequence in fragments shorter than   92228 bp
  25% of sequence in fragments shorter than  112279 bp
  30% of sequence in fragments shorter than  132783 bp
  35% of sequence in fragments shorter than  157126 bp
  40% of sequence in fragments shorter than  183597 bp
  45% of sequence in fragments shorter than  210603 bp
  50% of sequence in fragments shorter than  240081 bp
  55% of sequence in fragments shorter than  275535 bp
  60% of sequence in fragments shorter than  309526 bp
  65% of sequence in fragments shorter than  351396 bp
  70% of sequence in fragments shorter than  407058 bp
  75% of sequence in fragments shorter than  467218 bp
  80% of sequence in fragments shorter than  500000 bp

# NruI.SgrAI (TCGCGA+CRCCGGYG) double digest, 29 thousand fragments (14872+15270 sites), 99575 bp average fragment length
   5% of sequence in fragments shorter than   34440 bp
  10% of sequence in fragments shorter than   54979 bp
  15% of sequence in fragments shorter than   74756 bp
  20% of sequence in fragments shorter than   94189 bp
  25% of sequence in fragments shorter than  113679 bp
  30% of sequence in fragments shorter than  134909 bp
  35% of sequence in fragments shorter than  157516 bp
  40% of sequence in fragments shorter than  181607 bp
  45% of sequence in fragments shorter than  208388 bp
  50% of sequence in fragments shorter than  239586 bp
  55% of sequence in fragments shorter than  271239 bp
  60% of sequence in fragments shorter than  307993 bp
  65% of sequence in fragments shorter than  353262 bp
  70% of sequence in fragments shorter than  404890 bp
  75% of sequence in fragments shorter than  462597 bp
  80% of sequence in fragments shorter than  500000 bp

# PvuI.SgrAI (CGATCG+CRCCGGYG) double digest, 29 thousand fragments (14186+15270 sites), 101925 bp average fragment length
   5% of sequence in fragments shorter than   35989 bp
  10% of sequence in fragments shorter than   57931 bp
  15% of sequence in fragments shorter than   78889 bp
  20% of sequence in fragments shorter than   99669 bp
  25% of sequence in fragments shorter than  120748 bp
  30% of sequence in fragments shorter than  144192 bp
  35% of sequence in fragments shorter than  167763 bp
  40% of sequence in fragments shorter than  195332 bp
  45% of sequence in fragments shorter than  223944 bp
  50% of sequence in fragments shorter than  252234 bp
  55% of sequence in fragments shorter than  283787 bp
  60% of sequence in fragments shorter than  324917 bp
  65% of sequence in fragments shorter than  370389 bp
  70% of sequence in fragments shorter than  419770 bp
  75% of sequence in fragments shorter than  486729 bp
  80% of sequence in fragments shorter than  500000 bp

# NruI.PvuI (TCGCGA+CGATCG) double digest, 28 thousand fragments (14872+14186 sites), 103400 bp average fragment length
   5% of sequence in fragments shorter than   36081 bp
  10% of sequence in fragments shorter than   57356 bp
  15% of sequence in fragments shorter than   76785 bp
  20% of sequence in fragments shorter than   96173 bp
  25% of sequence in fragments shorter than  114706 bp
  30% of sequence in fragments shorter than  134660 bp
  35% of sequence in fragments shorter than  156470 bp
  40% of sequence in fragments shorter than  179495 bp
  45% of sequence in fragments shorter than  206234 bp
  50% of sequence in fragments shorter than  233588 bp
  55% of sequence in fragments shorter than  261705 bp
  60% of sequence in fragments shorter than  298033 bp
  65% of sequence in fragments shorter than  336306 bp
  70% of sequence in fragments shorter than  380701 bp
  75% of sequence in fragments shorter than  435516 bp
  80% of sequence in fragments shorter than  500000 bp

# FseI.SgrAI (GGCCGGCC+CRCCGGYG) double digest, 28 thousand fragments (13662+15270 sites), 103670 bp average fragment length
   5% of sequence in fragments shorter than   35209 bp
  10% of sequence in fragments shorter than   60345 bp
  15% of sequence in fragments shorter than   85310 bp
  20% of sequence in fragments shorter than  110300 bp
  25% of sequence in fragments shorter than  137225 bp
  30% of sequence in fragments shorter than  164806 bp
  35% of sequence in fragments shorter than  195416 bp
  40% of sequence in fragments shorter than  229883 bp
  45% of sequence in fragments shorter than  267360 bp
  50% of sequence in fragments shorter than  312737 bp
  55% of sequence in fragments shorter than  361331 bp
  60% of sequence in fragments shorter than  417353 bp
  65% of sequence in fragments shorter than  482980 bp
  70% of sequence in fragments shorter than  500000 bp

# FseI.NruI (GGCCGGCC+TCGCGA) double digest, 28 thousand fragments (13662+14872 sites), 105214 bp average fragment length
   5% of sequence in fragments shorter than   34166 bp
  10% of sequence in fragments shorter than   57328 bp
  15% of sequence in fragments shorter than   78869 bp
  20% of sequence in fragments shorter than  100380 bp
  25% of sequence in fragments shorter than  122402 bp
  30% of sequence in fragments shorter than  146874 bp
  35% of sequence in fragments shorter than  172671 bp
  40% of sequence in fragments shorter than  202115 bp
  45% of sequence in fragments shorter than  234657 bp
  50% of sequence in fragments shorter than  268871 bp
  55% of sequence in fragments shorter than  305723 bp
  60% of sequence in fragments shorter than  350247 bp
  65% of sequence in fragments shorter than  396785 bp
  70% of sequence in fragments shorter than  458739 bp
  75% of sequence in fragments shorter than  500000 bp

# FseI.PvuI (GGCCGGCC+CGATCG) double digest, 27 thousand fragments (13662+14186 sites), 107822 bp average fragment length
   5% of sequence in fragments shorter than   35832 bp
  10% of sequence in fragments shorter than   59938 bp
  15% of sequence in fragments shorter than   83028 bp
  20% of sequence in fragments shorter than  107132 bp
  25% of sequence in fragments shorter than  132320 bp
  30% of sequence in fragments shorter than  159308 bp
  35% of sequence in fragments shorter than  187451 bp
  40% of sequence in fragments shorter than  216858 bp
  45% of sequence in fragments shorter than  249135 bp
  50% of sequence in fragments shorter than  288122 bp
  55% of sequence in fragments shorter than  329599 bp
  60% of sequence in fragments shorter than  375060 bp
  65% of sequence in fragments shorter than  433494 bp
  70% of sequence in fragments shorter than  497673 bp
  75% of sequence in fragments shorter than  500000 bp

# AscI.MluI (GGCGCGCC+ACGCGT) double digest, 25 thousand fragments (4751+21415 sites), 114849 bp average fragment length
   5% of sequence in fragments shorter than   37950 bp
  10% of sequence in fragments shorter than   62045 bp
  15% of sequence in fragments shorter than   86641 bp
  20% of sequence in fragments shorter than  109364 bp
  25% of sequence in fragments shorter than  133020 bp
  30% of sequence in fragments shorter than  157857 bp
  35% of sequence in fragments shorter than  183781 bp
  40% of sequence in fragments shorter than  213464 bp
  45% of sequence in fragments shorter than  244535 bp
  50% of sequence in fragments shorter than  280965 bp
  55% of sequence in fragments shorter than  317874 bp
  60% of sequence in fragments shorter than  360020 bp
  65% of sequence in fragments shorter than  416496 bp
  70% of sequence in fragments shorter than  475649 bp
  75% of sequence in fragments shorter than  500000 bp

# NotI.SgrAI (GCGGCCGC+CRCCGGYG) double digest, 24 thousand fragments (9795+15270 sites), 119765 bp average fragment length
   5% of sequence in fragments shorter than   44895 bp
  10% of sequence in fragments shorter than   75417 bp
  15% of sequence in fragments shorter than  104851 bp
  20% of sequence in fragments shorter than  136580 bp
  25% of sequence in fragments shorter than  167783 bp
  30% of sequence in fragments shorter than  203580 bp
  35% of sequence in fragments shorter than  240823 bp
  40% of sequence in fragments shorter than  281201 bp
  45% of sequence in fragments shorter than  328863 bp
  50% of sequence in fragments shorter than  382579 bp
  55% of sequence in fragments shorter than  443755 bp
  60% of sequence in fragments shorter than  500000 bp

# RsrII.SgrAI (CGGWCCG+CRCCGGYG) double digest, 24 thousand fragments (9764+15270 sites), 120011 bp average fragment length
   5% of sequence in fragments shorter than   42096 bp
  10% of sequence in fragments shorter than   69939 bp
  15% of sequence in fragments shorter than   97086 bp
  20% of sequence in fragments shorter than  124913 bp
  25% of sequence in fragments shorter than  152943 bp
  30% of sequence in fragments shorter than  184346 bp
  35% of sequence in fragments shorter than  218112 bp
  40% of sequence in fragments shorter than  252776 bp
  45% of sequence in fragments shorter than  292049 bp
  50% of sequence in fragments shorter than  338945 bp
  55% of sequence in fragments shorter than  389486 bp
  60% of sequence in fragments shorter than  445740 bp
  65% of sequence in fragments shorter than  500000 bp

# NotI.NruI (GCGGCCGC+TCGCGA) double digest, 24 thousand fragments (9795+14872 sites), 121846 bp average fragment length
   5% of sequence in fragments shorter than   42948 bp
  10% of sequence in fragments shorter than   70522 bp
  15% of sequence in fragments shorter than   95523 bp
  20% of sequence in fragments shorter than  120253 bp
  25% of sequence in fragments shorter than  148217 bp
  30% of sequence in fragments shorter than  177920 bp
  35% of sequence in fragments shorter than  208108 bp
  40% of sequence in fragments shorter than  243543 bp
  45% of sequence in fragments shorter than  280989 bp
  50% of sequence in fragments shorter than  323888 bp
  55% of sequence in fragments shorter than  368939 bp
  60% of sequence in fragments shorter than  422163 bp
  65% of sequence in fragments shorter than  482709 bp
  70% of sequence in fragments shorter than  500000 bp

# NruI.RsrII (TCGCGA+CGGWCCG) double digest, 24 thousand fragments (14872+9764 sites), 122046 bp average fragment length
   5% of sequence in fragments shorter than   41067 bp
  10% of sequence in fragments shorter than   66256 bp
  15% of sequence in fragments shorter than   89645 bp
  20% of sequence in fragments shorter than  113164 bp
  25% of sequence in fragments shorter than  138413 bp
  30% of sequence in fragments shorter than  164036 bp
  35% of sequence in fragments shorter than  192590 bp
  40% of sequence in fragments shorter than  222375 bp
  45% of sequence in fragments shorter than  256942 bp
  50% of sequence in fragments shorter than  291924 bp
  55% of sequence in fragments shorter than  333990 bp
  60% of sequence in fragments shorter than  380635 bp
  65% of sequence in fragments shorter than  434603 bp
  70% of sequence in fragments shorter than  498494 bp
  75% of sequence in fragments shorter than  500000 bp

# NotI.PvuI (GCGGCCGC+CGATCG) double digest, 23 thousand fragments (9795+14186 sites), 125368 bp average fragment length
   5% of sequence in fragments shorter than   45581 bp
  10% of sequence in fragments shorter than   75458 bp
  15% of sequence in fragments shorter than  102510 bp
  20% of sequence in fragments shorter than  129571 bp
  25% of sequence in fragments shorter than  159004 bp
  30% of sequence in fragments shorter than  191397 bp
  35% of sequence in fragments shorter than  224952 bp
  40% of sequence in fragments shorter than  258214 bp
  45% of sequence in fragments shorter than  296944 bp
  50% of sequence in fragments shorter than  342251 bp
  55% of sequence in fragments shorter than  391487 bp
  60% of sequence in fragments shorter than  446959 bp
  65% of sequence in fragments shorter than  500000 bp

# PvuI.RsrII (CGATCG+CGGWCCG) double digest, 23 thousand fragments (14186+9764 sites), 125621 bp average fragment length
   5% of sequence in fragments shorter than   43853 bp
  10% of sequence in fragments shorter than   70554 bp
  15% of sequence in fragments shorter than   95799 bp
  20% of sequence in fragments shorter than  122293 bp
  25% of sequence in fragments shorter than  148177 bp
  30% of sequence in fragments shorter than  176045 bp
  35% of sequence in fragments shorter than  207067 bp
  40% of sequence in fragments shorter than  237991 bp
  45% of sequence in fragments shorter than  273894 bp
  50% of sequence in fragments shorter than  312891 bp
  55% of sequence in fragments shorter than  356002 bp
  60% of sequence in fragments shorter than  403435 bp
  65% of sequence in fragments shorter than  461204 bp
  70% of sequence in fragments shorter than  500000 bp

# FseI.NotI (GGCCGGCC+GCGGCCGC) double digest, 23 thousand fragments (13662+9795 sites), 128001 bp average fragment length
   5% of sequence in fragments shorter than   43990 bp
  10% of sequence in fragments shorter than   78196 bp
  15% of sequence in fragments shorter than  111442 bp
  20% of sequence in fragments shorter than  147549 bp
  25% of sequence in fragments shorter than  184627 bp
  30% of sequence in fragments shorter than  228173 bp
  35% of sequence in fragments shorter than  275900 bp
  40% of sequence in fragments shorter than  333638 bp
  45% of sequence in fragments shorter than  397446 bp
  50% of sequence in fragments shorter than  473842 bp
  55% of sequence in fragments shorter than  500000 bp

# FseI.RsrII (GGCCGGCC+CGGWCCG) double digest, 23 thousand fragments (13662+9764 sites), 128271 bp average fragment length
   5% of sequence in fragments shorter than   41830 bp
  10% of sequence in fragments shorter than   72349 bp
  15% of sequence in fragments shorter than  103175 bp
  20% of sequence in fragments shorter than  136998 bp
  25% of sequence in fragments shorter than  172507 bp
  30% of sequence in fragments shorter than  207203 bp
  35% of sequence in fragments shorter than  248307 bp
  40% of sequence in fragments shorter than  295783 bp
  45% of sequence in fragments shorter than  345297 bp
  50% of sequence in fragments shorter than  399507 bp
  55% of sequence in fragments shorter than  472742 bp
  60% of sequence in fragments shorter than  500000 bp

# AscI.SgrAI (GGCGCGCC+CRCCGGYG) double digest, 19 thousand fragments (4751+15270 sites), 150201 bp average fragment length
   5% of sequence in fragments shorter than   53841 bp
  10% of sequence in fragments shorter than   88978 bp
  15% of sequence in fragments shorter than  123129 bp
  20% of sequence in fragments shorter than  156805 bp
  25% of sequence in fragments shorter than  194534 bp
  30% of sequence in fragments shorter than  232252 bp
  35% of sequence in fragments shorter than  273319 bp
  40% of sequence in fragments shorter than  319325 bp
  45% of sequence in fragments shorter than  373815 bp
  50% of sequence in fragments shorter than  430180 bp
  55% of sequence in fragments shorter than  498357 bp
  60% of sequence in fragments shorter than  500000 bp

# AscI.NruI (GGCGCGCC+TCGCGA) double digest, 19 thousand fragments (4751+14872 sites), 153481 bp average fragment length
   5% of sequence in fragments shorter than   52801 bp
  10% of sequence in fragments shorter than   84173 bp
  15% of sequence in fragments shorter than  112114 bp
  20% of sequence in fragments shorter than  140857 bp
  25% of sequence in fragments shorter than  171113 bp
  30% of sequence in fragments shorter than  201832 bp
  35% of sequence in fragments shorter than  237269 bp
  40% of sequence in fragments shorter than  275035 bp
  45% of sequence in fragments shorter than  315572 bp
  50% of sequence in fragments shorter than  361250 bp
  55% of sequence in fragments shorter than  410007 bp
  60% of sequence in fragments shorter than  468463 bp
  65% of sequence in fragments shorter than  500000 bp

# NotI.RsrII (GCGGCCGC+CGGWCCG) double digest, 19 thousand fragments (9795+9764 sites), 153869 bp average fragment length
   5% of sequence in fragments shorter than   56036 bp
  10% of sequence in fragments shorter than   95537 bp
  15% of sequence in fragments shorter than  134600 bp
  20% of sequence in fragments shorter than  174811 bp
  25% of sequence in fragments shorter than  215433 bp
  30% of sequence in fragments shorter than  263117 bp
  35% of sequence in fragments shorter than  315525 bp
  40% of sequence in fragments shorter than  377392 bp
  45% of sequence in fragments shorter than  447928 bp
  50% of sequence in fragments shorter than  500000 bp

# AscI.PvuI (GGCGCGCC+CGATCG) double digest, 18 thousand fragments (4751+14186 sites), 159145 bp average fragment length
   5% of sequence in fragments shorter than   56961 bp
  10% of sequence in fragments shorter than   90827 bp
  15% of sequence in fragments shorter than  122464 bp
  20% of sequence in fragments shorter than  154700 bp
  25% of sequence in fragments shorter than  188872 bp
  30% of sequence in fragments shorter than  225044 bp
  35% of sequence in fragments shorter than  259783 bp
  40% of sequence in fragments shorter than  301271 bp
  45% of sequence in fragments shorter than  345851 bp
  50% of sequence in fragments shorter than  395941 bp
  55% of sequence in fragments shorter than  449715 bp
  60% of sequence in fragments shorter than  500000 bp

# AscI.FseI (GGCGCGCC+GGCCGGCC) double digest, 18 thousand fragments (4751+13662 sites), 163377 bp average fragment length
   5% of sequence in fragments shorter than   53907 bp
  10% of sequence in fragments shorter than   93804 bp
  15% of sequence in fragments shorter than  132177 bp
  20% of sequence in fragments shorter than  174697 bp
  25% of sequence in fragments shorter than  215650 bp
  30% of sequence in fragments shorter than  269576 bp
  35% of sequence in fragments shorter than  327488 bp
  40% of sequence in fragments shorter than  391080 bp
  45% of sequence in fragments shorter than  466450 bp
  50% of sequence in fragments shorter than  500000 bp

# AscI.NotI (GGCGCGCC+GCGGCCGC) double digest, 14 thousand fragments (4751+9795 sites), 207333 bp average fragment length
   5% of sequence in fragments shorter than   78382 bp
  10% of sequence in fragments shorter than  134492 bp
  15% of sequence in fragments shorter than  188460 bp
  20% of sequence in fragments shorter than  246724 bp
  25% of sequence in fragments shorter than  313963 bp
  30% of sequence in fragments shorter than  394333 bp
  35% of sequence in fragments shorter than  480014 bp
  40% of sequence in fragments shorter than  500000 bp

# AscI.RsrII (GGCGCGCC+CGGWCCG) double digest, 14 thousand fragments (4751+9764 sites), 208027 bp average fragment length
   5% of sequence in fragments shorter than   70867 bp
  10% of sequence in fragments shorter than  120509 bp
  15% of sequence in fragments shorter than  168047 bp
  20% of sequence in fragments shorter than  213068 bp
  25% of sequence in fragments shorter than  266084 bp
  30% of sequence in fragments shorter than  323919 bp
  35% of sequence in fragments shorter than  391616 bp
  40% of sequence in fragments shorter than  461477 bp
  45% of sequence in fragments shorter than  500000 bp
 




Posfai@neb.com

September 23, 2015