# ENZYME SPECIFICITY SITE_COUNT AVE_FRAG_LENGTH SITES_PER_MEGABP AatII GACGTC 71089 41323 24.2 AccI GTMKAC 1101063 2668 374.8 AciI CCGC 4206130 698 1431.8 AclI AACGTT 172706 17009 58.8 AflII CTTAAG 643284 4566 219.0 AflIII ACRYGT 1485394 1977 505.6 AgeI ACCGGT 53386 55026 18.2 AluI AGCT 13027778 225 4434.8 ApaI GGGCCC 460339 6381 156.7 ApaLI GTGCAC 496255 5919 168.9 ApoI RAATTY 6382433 460 2172.6 AscI GGCGCGCC 4751 618320 1.6 AvaI CYCGRG 1005714 2920 342.4 AvaII GGWCC 2747690 1069 935.3 AvaIII ATGCAT 933357 3147 317.7 AvrII CCTAGG 594956 4937 202.5 BamHI GGATCC 365999 8026 124.6 BbvI GCAGC 5290051 555 1800.8 BccI CCATC 6170927 476 2100.6 BceAI ACGGC 705178 4165 240.0 BciVI GTATCC 696093 4220 237.0 BclI TGATCA 738785 3976 251.5 BfiI ACTGGG 1706594 1721 580.9 BglII AGATCT 774732 3791 263.7 BsaAI YACGTR 713801 4115 243.0 BscAI GCATC 3652926 804 1243.5 BscGI CCCGT 1231070 2386 419.1 BsmAI GTCTC 6631585 442 2257.5 BsmI GAATGC 1549350 1896 527.4 BspHI TCATGA 978290 3002 333.0 BspKT6I GATC 7199434 408 2450.8 BspMI ACCTGC 1438129 2042 489.6 BspNCI CCAGA 7282436 403 2479.0 BsrBI CCGCTC 235523 12472 80.2 BsrI ACTGG 5741310 511 1954.4 BstEII GGTNACC 335865 8746 114.3 BstNI CCWGG 9855646 298 3355.0 BthCI GCNGC 6209929 473 2113.9 ClaI ATCGAT 86477 33970 29.4 CviAII CATG 13815697 212 4703.0 CviQI GTAC 5115078 574 1741.2 DdeI CTNAG 14312045 205 4872.0 EciI GGCGGA 571273 5142 194.5 EcoRI GAATTC 847401 3466 288.5 EcoRV GATATC 446473 6579 152.0 EsaBC3I TCGA 1603346 1832 545.8 FauI CCCGC 1439772 2040 490.1 FnuDII CGCG 733940 4002 249.8 FokI GGATG 5201116 564 1770.5 FseI GGCCGGCC 13662 215022 4.7 HaeII RGCGCY 630101 4662 214.5 HaeIII GGCC 8562230 343 2914.7 HgaI GACGC 571889 5136 194.7 HgiCI GGYRCC 1550877 1894 527.9 HhaI GCGC 1756502 1672 597.9 Hin4II CCTTC 7059915 416 2403.3 HindIII AAGCTT 860365 3414 292.9 HinfI GANTC 8916054 329 3035.1 HpaI GTTAAC 389418 7543 132.6 HpaII CCGG 2317722 1267 789.0 HphI GGTGA 6007336 489 2045.0 Hpy188I TCNGA 8942153 328 3044.0 Hpy99I CGWCG 157917 18602 53.8 KasI GGCGCC 230381 12751 78.4 KpnI GGTACC 287974 10201 98.0 MboII GAAGA 9199496 319 3131.6 MfeI CAATTG 564303 5205 192.1 MluI ACGCGT 21415 137176 7.3 MlyI GAGTC 3931692 747 1338.4 MnlI CCTC 27739501 105 9442.8 MseI TTAA 19214674 152 6540.9 NaeI GCCGGC 125692 23371 42.8 NcoI CCATGG 759594 3867 258.6 NdeI CATATG 903360 3251 307.5 NheI GCTAGC 287384 10221 97.8 NotI GCGGCCGC 9795 299912 3.3 NruI TCGCGA 14872 197528 5.1 NspI RCATGY 3104359 946 1056.8 PacI TTAATTAA 159455 18422 54.3 PmeI GTTTAAAC 42094 69787 14.3 PpuMI RGGWCCY 1085138 2707 369.4 PsiI TTATAA 1647912 1782 561.0 PspXI VCTCGAGB 59193 49628 20.1 PstI CTGCAG 1321469 2223 449.8 PvuI CGATCG 14186 207080 4.8 PvuII CAGCTG 1084260 2709 369.1 RsrII CGGWCCG 9764 300864 3.3 SacI GAGCTC 603323 4869 205.4 SacII CCGCGG 66901 43910 22.8 SalI GTCGAC 31396 93567 10.7 SapI GCTCTTC 377161 7788 128.4 ScaI AGTACT 543793 5402 185.1 ScrFI CCNGG 11805099 248 4018.6 SexAI ACCWGGT 297900 9861 101.4 SfeI CTRYAG 3531009 831 1202.0 SgrAI CRCCGGYG 15270 192379 5.2 SmaI CCCGGG 376940 7793 128.3 SmlI CTYRAG 2727867 1076 928.6 SnaBI TACGTA 132299 22204 45.0 SpeI ACTAGT 400286 7338 136.3 SphI GCATGC 550952 5331 187.5 SspI AATATT 2377267 1235 809.2 StuI AGGCCT 925729 3173 315.1 StyI CCWWGG 3114107 943 1060.1 SwaI ATTTAAAT 226602 12963 77.1 TfiI GAWTC 4984362 589 1696.7 TseI GCWGC 5290051 555 1800.8 Tsp45I GTSAC 4021761 730 1369.0 TspRI CASTG 8765240 335 2983.8 XbaI TCTAGA 850998 3451 289.7 XhoI CTCGAG 121308 24216 41.3