# Nothobranchius furzeri genome, 1242.499 Mbp of sequence in 5896 scaffolds # # ENZYME SPECIFICITY SITES_COUNT SITES_PER_MEGABASE AVE_FRAG_LENGTH (bp) # AatII GACGTC 202131 162.68 6146 Acc65I GGTACC 187241 150.70 6635 AccI GTMKAC 693072 557.81 1792 AciI CCGC 4641981 3736.01 267 AclI AACGTT 383776 308.87 3237 AcuI CTGAAG 1640810 1320.57 757 AfeI AGCGCT 262956 211.63 4725 AflII CTTAAG 283511 228.18 4382 AflIII ACRYGT 1534278 1234.83 809 AgeI ACCGGT 215768 173.66 5758 AluI AGCT 8986254 7232.41 138 AlwI GGATC 2176899 1752.03 570 ApaI GGGCCC 154927 124.69 8019 ApaLI GTGCAC 348058 280.13 3569 ApeKI GCWGC 5189118 4176.36 239 ApoI RAATTY 2541279 2045.30 488 AscI GGCGCGCC 2371 1.91 524039 AseI ATTAAT 859466 691.72 1445 AsiSI GCGATCGC 1272 1.02 976807 AvaI CYCGRG 628288 505.66 1977 AvaII GGWCC 2200505 1771.03 564 AvrII CCTAGG 113715 91.52 10926 BaeGI GKGCMC 890684 716.85 1394 BamHI GGATCC 243543 196.01 5101 BanI GGYRCC 760700 612.23 1633 BanII GRGCYC 1145776 922.15 1084 BbvI GCAGC 5189118 4176.36 239 BccI CCATC 3815972 3071.21 325 BceAI ACGGC 1240250 998.19 1001 BciVI GTATCC 323513 260.37 3840 BclI TGATCA 621124 499.90 2000 BcoDI GTCTC 3208315 2582.15 387 BfuAI ACCTGC 1045587 841.52 1188 BfuCI GATC 4886232 3932.59 254 BglII AGATCT 497702 400.57 2496 BlpI GCTNAGC 426459 343.23 2913 BmrI ACTGGG 713814 574.50 1740 BmtI GCTAGC 249479 200.79 4980 BpmI CTGGAG 1546942 1245.03 803 BpuEI CTTGAG 736970 593.14 1685 BsaAI YACGTR 741134 596.49 1676 BsaHI GRCGYC 662562 533.25 1875 BsaI GGTCTC 663546 534.04 1872 BsaWI WCCGGW 783443 630.54 1585 BseRI GAGGAG 1840781 1481.52 674 BsgI GTGCAG 1108777 892.38 1120 BsiEI CGRYCG 372437 299.75 3336 BsiHKAI GWGCWC 1545497 1243.86 803 BsiWI CGTACG 54836 44.13 22658 BsmAI GTCTC 3208315 2582.15 387 BsmBI CGTCTC 586455 472.00 2118 BsmFI GGGAC 2126563 1711.52 584 BsmI GAATGC 697227 561.15 1782 BsoBI CYCGRG 628288 505.66 1977 Bsp1286I GDGCHC 2610907 2101.34 475 BspCNI CTCAG 4746101 3819.80 261 BspDI ATCGAT 190001 152.92 6539 BspEI TCCGGA 169053 136.06 7349 BspHI TCATGA 583421 469.55 2129 BspMI ACCTGC 1045587 841.52 1188 BspQI GCTCTTC 247191 198.95 5026 BsrBI CCGCTC 456779 367.63 2720 BsrFI RCCGGY 689341 554.80 1802 BsrGI TGTACA 465243 374.44 2670 BsrI ACTGG 3537515 2847.10 351 BssHII GCGCGC 116188 93.51 10693 BssSI CACGAG 435156 350.23 2855 BstBI TTCGAA 98036 78.90 12673 BstEII GGTNACC 249867 201.10 4972 BstNI CCWGG 3551031 2857.98 349 BstUI CGCG 1198539 964.62 1036 BstYI RGATCY 1441603 1160.25 861 BstZ17I GTATAC 112211 90.31 11072 Bsu36I CCTNAGG 343088 276.13 3621 BtgI CCRYGG 779010 626.97 1594 BtgZI GCGATG 288084 231.86 4312 BtsCI GGATG 3789889 3050.22 327 ClaI ATCGAT 190001 152.92 6539 CviAII CATG 7834126 6305.14 158 CviKI-1 RGCY 23298846 18751.61 53 CviQI GTAC 3137351 2525.03 396 DdeI CTNAG 6536640 5260.88 190 DpnII GATC 4886232 3932.59 254 DraI TTTAAA 2236825 1800.26 555 EaeI YGGCCR 918031 738.86 1353 EagI CGGCCG 136222 109.64 9121 EarI CTCTTC 1176177 946.62 1056 EciI GGCGGA 357209 287.49 3478 Eco53kI GAGCTC 468065 376.71 2654 EcoO109I RGGNCCY 956305 769.66 1299 EcoRI GAATTC 294120 236.72 4224 EcoRV GATATC 211629 170.33 5871 FatI CATG 7834126 6305.14 158 FauI CCCGC 1105542 889.77 1123 Fnu4HI GCNGC 6252701 5032.36 198 FokI GGATG 3789889 3050.22 327 FseI GGCCGGCC 4172 3.36 297818 FspI TGCGCA 162575 130.85 7642 HaeII RGCGCY 807667 650.03 1538 HaeIII GGCC 3651798 2939.08 340 HgaI GACGC 1281979 1031.78 969 HhaI GCGC 2178223 1753.10 570 HincII GTYRAC 1113222 895.95 1116 HindIII AAGCTT 532841 428.85 2331 HinfI GANTC 5289926 4257.49 234 HinP1I GCGC 2178223 1753.10 570 HpaI GTTAAC 323978 260.75 3835 HpaII CCGG 2699585 2172.71 460 HphI GGTGA 3720793 2994.61 333 Hpy188I TCNGA 7404053 5959.00 167 Hpy99I CGWCG 639007 514.29 1944 HpyAV CCTTC 3395742 2732.99 365 HpyCH4III ACNGT 5664590 4559.03 219 HpyCH4IV ACGT 3583470 2884.08 346 HpyCH4V TGCA 9347813 7523.40 132 KasI GGCGCC 135169 108.79 9192 KpnI GGTACC 187241 150.70 6635 MboI GATC 4886232 3932.59 254 MboII GAAGA 4676675 3763.93 265 MfeI CAATTG 251130 202.12 4947 MluCI AATT 9424256 7584.92 131 MluI ACGCGT 84298 67.85 14739 MlyI GAGTC 2787909 2243.79 445 MmeI TCCRAC 1325125 1066.50 937 MnlI CCTC 14218601 11443.56 87 MscI TGGCCA 432586 348.16 2872 MseI TTAA 12011770 9667.43 103 MspA1I CMGCKG 1846680 1486.26 672 MspI CCGG 2699585 2172.71 460 NaeI GCCGGC 174758 140.65 7109 NarI GGCGCC 135169 108.79 9192 NciI CCSGG 1336087 1075.32 929 NcoI CCATGG 349895 281.61 3551 NdeI CATATG 296142 238.34 4195 NgoMIV GCCGGC 174758 140.65 7109 NheI GCTAGC 249479 200.79 4980 NlaIII CATG 7834126 6305.14 158 NmeAIII GCCGAG 345414 278.00 3597 NotI GCGGCCGC 6686 5.38 185835 NruI TCGCGA 45300 36.46 27428 NsiI ATGCAT 620896 499.72 2001 NspI RCATGY 2220900 1787.45 559 PacI TTAATTAA 55095 44.34 22551 PaeR7I CTCGAG 141845 114.16 8759 PciI ACATGT 763717 614.66 1626 PleI GAGTC 2787909 2243.79 445 PluTI GGCGCC 135169 108.79 9192 PmeI GTTTAAAC 28385 22.85 43773 PmlI CACGTG 306766 246.89 4050 PpuMI RGGWCCY 579651 466.52 2143 PsiI TTATAA 753872 606.74 1648 PspGI CCWGG 3551031 2857.98 349 PspOMI GGGCCC 154927 124.69 8019 PspXI VCTCGAGB 42935 34.56 28939 PstI CTGCAG 1249705 1005.80 994 PvuI CGATCG 63595 51.18 19537 PvuII CAGCTG 1207473 971.81 1029 RsaI GTAC 3137351 2525.03 396 RsrII CGGWCCG 52125 41.95 23836 SacI GAGCTC 468065 376.71 2654 SacII CCGCGG 86367 69.51 14386 SalI GTCGAC 149587 120.39 8306 SapI GCTCTTC 247191 198.95 5026 Sau3AI GATC 4886232 3932.59 254 Sau96I GGNCC 3842718 3092.73 323 SbfI CCTGCAGG 31824 25.61 39042 ScaI AGTACT 239149 192.47 5195 ScrFI CCNGG 4887118 3933.30 254 SexAI ACCWGGT 225143 181.20 5518 SfaNI GCATC 2966565 2387.58 418 SfcI CTRYAG 2434744 1959.55 510 SfoI GGCGCC 135169 108.79 9192 SgrAI CRCCGGYG 16375 13.18 75877 SmaI CCCGGG 161487 129.97 7694 SmlI CTYRAG 1162326 935.47 1068 SnaBI TACGTA 118357 95.26 10497 SpeI ACTAGT 271154 218.23 4582 SphI GCATGC 405063 326.01 3067 SrfI GCCCGGGC 10166 8.18 122220 SspI AATATT 1091971 878.85 1137 StuI AGGCCT 298537 240.27 4161 StyD4I CCNGG 4887118 3933.30 254 StyI CCWWGG 1160931 934.35 1070 SwaI ATTTAAAT 84220 67.78 14753 TaqI TCGA 2018785 1624.78 615 TfiI GAWTC 2502017 2013.70 496 TseI GCWGC 5189118 4176.36 239 Tsp45I GTSAC 3224323 2595.03 385 TspMI CCCGGG 161487 129.97 7694 TspRI CASTG 4624941 3722.29 268 XbaI TCTAGA 339768 273.46 3656 XhoI CTCGAG 141845 114.16 8759 XmaI CCCGGG 161487 129.97 7694 ZraI GACGTC 202131 162.68 6146