Ref: Johnson,T.J. and Isaacson,R.E. unpublished

REBASE ref # 12619

Sequence length:GenBank #:
Chromosome: 4,517,924 bp FBSE00000000 (NZ_FBSE00000000)
chromosome I: 5,144,250 bp LT601384
chromosome RL465_chromosome: 4,894,900 bp LT594504
plasmid pPWD4_103: 103,297 bp HQ114284

REBASE acronym: EcoPWD4

Org_num: 13158

 

All begin JF733_, NCTC86EC_, PWD4IncI1_

Type I
ORF Gene Most similar Specificity Name
 
Chromosome
 
4211 endoribonuclease SymE
4212 S S.SenHNK130ORF17125P (100% identity) AACNNNNNNGTGC S.EcoJF733ORF4213P
4213 M M.SenHNK130ORF17125P (100% identity) AACNNNNNNGTGC M.EcoJF733ORF4213P
??? R Msa17ORFC2P (100% identity) AACNNNNNNGTGC EcoJF733ORF4213P
4215 EcoKMrr
 
chromosome I
 
2109 81 aa hypothetical protein
2110 M M.EcosB2CORFEP (100% identity) M.Eco86ECORF2110P
2111 type I restriction-modification enzyme, S
2112 S S.EcosB2CORFEP (86% identity) S.Eco86ECORF2110P
2113 218 aa hypothetical protein
2114 RNase III inhibitor
2115 R PmeBin330ORFCP (100% identity) Eco86ECORF2110P
2116 SprT-like family protein
 
2138 transposase subunit
??? S S2.EcoSTEC416ORF10780P (62% identity) S.Eco86ECORFAP
2140 217 aa hypothetical protein
 
2143 83 aa hypothetical protein
??? R PmeBin330ORFCP (94% identity) Eco86ECORF2144P
??? S S2.EcoMEC0001ORF18360P (95% identity) S.Eco86ECORF2144P
2152 IS66 family orf1
 
chromosome RL465_chromosome
 
??? M M.SenS688ORF13495P (100% identity) M.EcoRL465ORFCP
??? S S.Sen12509ORF5865P (100% identity) S.EcoRL465ORFCP
??? R Sen12509ORF5865P (100% identity) EcoRL465ORFCP
Type II
ORF Gene Most similar Specificity Name
 
Chromosome
 
3257 Ribulose-phosphate 3-epimerase
3258 M M.UbaC1152DamP (100% identity) GATC M.EcoJF733DamP
3259 cell division protein DamX
 
3135 Hin recombinational enhancer-binding protein
3136 M M.SflSTLE4ORF2240P (100% identity) ATGCAT M.EcoJF733ORF3136P
3137 59 aa hypothetical protein
 
1870 Very short patch repair protein
1871 M M.SflLIN6DcmP (100% identity) CCWGG M.EcoJF733DcmP
1872 hydrolase
 
chromosome I
 
888 metal dependent phosphohydrolase
889 M M.SflN116DcmP (100% identity) CCWGG M.Eco86ECDcmP
??? V V.EcoO146DcmP (100% identity) V.Eco86ECDcmP
891 carboxylate/amino acid/amine transporter
 
1609 pseudogene
1612 M M.Spn7978ORF3515P (100% identity) A M.Eco86ECORF1612P
1613 lipoprotein
 
2924 568 aa hypothetical protein
2925 M M.EcosEC31ORF18555P (100% identity) GGTACC M.Eco86ECORF2925P
2926 site-specific recombinase, phage integrase
 
3759 DNA-binding protein Fis
3760 M M.SflSTLE4ORF2240P (100% identity) ATGCAT M.Eco86ECORF3760P
3761 membrane protein
 
3889 ribulose-phosphate 3-epimerase
3890 M M.SsoST152DamP (100% identity) GATC M.Eco86ECDamP
3891 sporulation domain-containing protein
 
4934 histidine kinase-like protein
4935 M M.Sen5773ORF4471P (100% identity) RCCGGY M.Eco86ECORF4935P
4936 transposase
 
chromosome RL465_chromosome
 
??? M M.EcosE2ORF21575P (100% identity) GGCC M.EcoRL465ORFHP
 
??? M M.Sso90ORF15820P (99% identity) GATC M.EcoRL465ORFFP
??? DNA adenine methylase
 
??? phage N-6-adenine-methyltransferase
??? M M.Sen38ORF10355P (100% identity) M.EcoRL465ORFGP
 
??? V V.Sdy49550DcmP (100% identity) V.EcoRL465DcmP
??? M M.SflSTLE1DcmP (100% identity) CCWGG M.EcoRL465DcmP
 
??? M M.Ssp28760ORF20045P (100% identity) ATGCAT M.EcoRL465ORFBP
 
plasmid pPWD4_103
 
31 101 aa hypothetical protein
32 M M.Sfl712783ORFFP (100% identity) M.EcoPWD4ORF32P
33 144 aa hypothetical protein
Type III
ORF Gene Most similar Specificity Name
 
chromosome I
 
5012 type III restriction enzyme, res subunit
5013 M M.Ecos19ORF20250P (100% identity) GAGCC M.Eco86ECORF5013P
5014 R Ecos19ORF20250P (100% identity) GAGCC Eco86ECORF5013P
5015 100 aa hypothetical protein
Type IV
ORF Gene Most similar Specificity Name
 
Chromosome
 
4208 906 aa hypothetical protein
4209 R SenHNK130McrCP (100% identity) EcoJF733McrCP
4210 R Msa17McrB2P (100% identity) EcoJF733McrBP
4211 endoribonuclease SymE
 
??? type I restriction-modification system deoxyribonuclease
4215 R SenHNK130MrrP (100% identity) EcoJF733MrrP
4216 GTP-binding protein YjiA
 
??? Mrr restriction system protein
4215 R SenHNK130MrrP (100% identity) EcoJF733Mrr2P
4216 GTP-binding protein YjiA
 
chromosome RL465_chromosome
 
??? R Ecos2020McrBP (100% identity) EcoRL465McrB2P
??? R EmaE2C08McrCP (100% identity) EcoRL465McrC2P
 
??? R EcoteDMcrBP (92% identity) EcoRL465McrBP
??? R EmaE2C08McrCP (100% identity) EcoRL465McrCP
Orphan M
ORF Gene Most similar Specificity Name
 
chromosome RL465_chromosome
 
??? M M.Sen663ORF16605P (100% identity) GATC M.EcoRL465ORFEP
 
??? M M.UbaC1152DamP (100% identity) GATC M.EcoRL465ORFAP
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